LOCUS       DASUTA010000001         2155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1684281, whole genome shotgun sequence.
ACCESSION   DASUTA010000001 DASUTA010000000
VERSION     DASUTA010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684281"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFZ80_00005"
     CDS             complement(<1..222)
                     /locus_tag="VFZ80_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285841.1"
                     /translation="MTTTSDTTSEETRSPRAIWLWPDRFGRWSNPALFVSVVVAYALG
                     AQVALLLIEASGLQGVLFIPSGITVAFLLR"
     gene            421..600
                     /locus_tag="VFZ80_00010"
     CDS             421..600
                     /locus_tag="VFZ80_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285842.1"
                     /translation="MFRDRFWLSLTLTIPVIFYSETIQGWFGYTAPSFPGSEWISPVP
                     GHGDLHLRRGTSPMS"
     gene            complement(647..1288)
                     /locus_tag="VFZ80_00015"
     CDS             complement(647..1288)
                     /locus_tag="VFZ80_00015"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX6285843.1"
                     /translation="MSVTLTTVEKLQIREAAERLRRRFEGQLNAETIERFMNDSLDTL
                     MQRAHTSTWIPLLAERFARDRLRALVRLESEDLTLKPSVLFLCVHNAGRSQMAAGWMR
                     HLGGDHVDVFSGGSEPAEIVNTAAVAAMAEKGIDISGEIPQPWADEIVRAADVVVTMG
                     CGDACPVFPGKRYVDWELEDPSGKTLEHVRPIRDDLEQRVRGLMAELEIPLTV"
     gene            complement(1301..1972)
                     /locus_tag="VFZ80_00020"
     CDS             complement(1301..1972)
                     /locus_tag="VFZ80_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017882775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX6285844.1"
                     /translation="MRRKLVAEALGTALLLYVVVGSGIVAQQLVGDPGVGLLAHALAV
                     GLGLGVLVAMLQAVSGAHFNPSVTLAFWRLRAIGRSQAAGFAAAQVAGGIVGVALANW
                     SFGEAPIALSSSERDGAGLLVAEAIATFVLVLVIVALVRTGRSQSVPAAVGAWVAAAI
                     FATSSTGFANPAVTLARAFTDTFTGIAPGSVPAFIAAQLLAGLLAALAALAIYPNRAA
                     EPATV"
     gene            complement(1969..>2155)
                     /locus_tag="VFZ80_00025"
     CDS             complement(1969..>2155)
                     /locus_tag="VFZ80_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEX6285845.1"
                     /translation="PGELDVVVSVCDRAHEAGVPRAGKTLHWSVPDPVPMGTVAAFRS
                     SFDDIARRIERLSATST"
BASE COUNT          424 a          701 c          662 g          368 t
ORIGIN      
        1 acgcagcaga aaggcaactg tgatgccgga gggtatgaac aggacaccct gaagccccga
       61 ggcctcgatg agaagcagag ccacctgtgc accaagggca tacgcgacga ctacagagac
      121 gaagagtgcc ggattgctcc atcgaccgaa gcggtcgggc cagagccaaa tcgcgcgagg
      181 cgacctcgtt tcctcggacg ttgtgtcgct ggtagtggtc aaagcggtga gttctgcgat
      241 acccgaggct ataccgctgt ttccaacctc tcagccacat aacgttggca ttgccgcggc
      301 agaacagctc catgacaggc caccatcacg accacgccgc ccattgagac gaccacgatc
      361 cagcggtcgt cacgccccac cacggcgggc acgacgagca acgaggggca cagtgtcgac
      421 atgtttcgcg atcggttctg gctgtcgctc acgctgacca tcccggtcat cttctacagc
      481 gagaccattc agggctggtt cggttacacg gctccctcgt tcccggggtc ggagtggatc
      541 tcgccagtcc ctgggcacgg tgatcttcac ctacggcggg gcacgtctcc gatgtcttga
      601 ccgaaggtgg gtctcgctca gatgctccct ggagatacgg cgccggtcac acggtgagcg
      661 gtatctcgag ctcggccatg aggcctcgca cccgttgctc cagatcatcg cgtatcggtc
      721 tgacgtgctc cagggtcttg ccggaggggt cctccagttc ccaatcgaca taccgcttgc
      781 ccggaaacac cgggcaggca tcgccacagc ccatcgtcac caccacgtcg gctgccctga
      841 cgatctcatc cgcccagggt tgggggatct ccccacttat gtcgatgccc ttttctgcca
      901 tggcggccac cgctgccgtg ttgacgatct cagcaggctc ggagccacca gagaacacgt
      961 cgacgtggtc accgccgagg tgccgcatcc atccagccgc catctgtgat cgtcccgcat
     1021 tgtgcacgca taggaagagg acactcggtt tgagagtcag gtcctcggat tcgagtctga
     1081 ccagggcgcg aagacggtcc ctggcgaacc gctcggcgag gagagggatc catgtcgagg
     1141 tgtgcgcccg ctgcatcaat gtgtcgaggg aatcgttcat gaaacgctcg atcgtctcgg
     1201 cattgagctg accctcgaac ctccgtctca gtctctcggc agcctctctg atctgaagct
     1261 tctcaacggt tgtcagcgtg accgacatat ctctctcctt ttagacagtg gcgggttctg
     1321 ccgctcgatt cgggtagatg gcgagcgccg cgagcgcggc cagcaggccg gcgaggagct
     1381 gcgcagcaat gaacgcgggc accgatccag gtgctattcc ggtgaaagtg tcggtgaagg
     1441 cgcgggcgag ggtcacagcc gggttggcga agcccgtcga cgaggtggcg aagatcgcag
     1501 cggccaccca tgccccgacg gcggcgggaa cgctctgcga tcggccggtt cgcacgagag
     1561 cgacgatcac cagtacgagg acgaaggtgg cgatggcctc tgcgaccaga agccctgctc
     1621 catccctctc cgacgaggag agagcgatcg gcgcttcacc gaagctccag ttggccagag
     1681 cgaccccgac gattccgccc gcgacctgtg ccgcggcgaa acctgcggct tgtgaccggc
     1741 cgatagccct cagacgccag aaagcgaggg tgaccgaagg attgaagtgc gcgcctgaga
     1801 ccgcttgcag catggcgacg agcacgccga gcccgagccc cacggccagc gcatgcgcca
     1861 acaatccgac accaggatcc ccgaccagtt gttgggcgac gatgccactt cccaccacga
     1921 catataggag caacgcggtg ccgagggcct ccgccaccaa cttgcgtctc acgttgatgt
     1981 ggcgctcagg cgttcgatgc gtcgggcaat gtcatcgaat gaggaccgga aggccgcaac
     2041 agttcccatc ggcacagggt caggaaccga ccaatgaagc gtcttaccgg ctcgaggaac
     2101 cccggcctcg tgcgcccggt cgcagacact cacgaccacg tccaactcac ctggt
//
LOCUS       DASUTA010000002         3156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_129876, whole genome shotgun sequence.
ACCESSION   DASUTA010000002 DASUTA010000000
VERSION     DASUTA010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129876"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..463
                     /locus_tag="VFZ80_00030"
     CDS             122..463
                     /locus_tag="VFZ80_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX6285846.1"
                     /translation="MRARATSAHEIPAREPIRLEPGHQVEVGEPDSQWPAFVFVTGPT
                     GQGWVPSRHLSADAGTAVVVTPYDTTELSLEAGEVVDVLDRDDESGWWWCRRGDGAQG
                     WVPVSALEPEK"
     gene            complement(523..598)
                     /locus_tag="VFZ80_00035"
     tRNA            complement(523..598)
                     /locus_tag="VFZ80_00035"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(562..564),aa:Glu,seq:ttc)
     gene            700..1302
                     /locus_tag="VFZ80_00040"
     CDS             700..1302
                     /locus_tag="VFZ80_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019526757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEX6285847.1"
                     /translation="MFATDGQHSLFAAEEASFDPDYTQLRRIQLDDTAWLDYSPMWLA
                     GDETLFHLLTERAEWRQPVVRMYDRDVLTPRLVARLQPDIHPVVPEMIDSLSSRYGVR
                     LDQVSAGWYRDGNDSVAYHGDRVAREREEAIVATVSLGAARRFLIKPKDRGGETHSFS
                     LGHGDLVVMGGSCQRTWEHAVPKAAAAGPRLALMFRHFYI"
     gene            complement(1307..2710)
                     /gene="gltX"
                     /locus_tag="VFZ80_00045"
     CDS             complement(1307..2710)
                     /gene="gltX"
                     /locus_tag="VFZ80_00045"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX6285848.1"
                     /translation="MAPSPTGFLHVGTARAALYNWLYARHEVGTFIVRIDDTDLERST
                     KEYEEEILGSFRWLGLDWDEGVEVGGPHGSYRQSDRIEHYAELARGLVESGHAYYDDR
                     TTEELDTLRDRAQKEGKHPGHYVRRPERETSSGVIRLSIPPDRAVEFDDLIRGHLSFA
                     PNDIDDFVILRSNGTPTYHLASTVDDVEYEITHVARGEDLLPSTPKHILLTRALGGDE
                     PTYAHLPLLFGTDGKKLSKRHGATALAEYRDQGFLPSAMFNYLAILGWSPDGETTIFD
                     RQEAVEKFDLVDVSKNPAAFDPEKLAWMNGEYIRAMDTTAFAELVRPYVEAEVGRALD
                     SEEWAKFGDIAPLVQERTRLLPEAGSQVTFLFEDFDAYDDQSWEKVMTKEGVVEVLEE
                     GRSAIAGLDTWDTMSVESALRAIPEKLGIGAGKTFQPMRVAVTGSSVSPPLFESISAL
                     GRERTLERIDRARAKLG"
     gene            complement(2725..>3156)
                     /locus_tag="VFZ80_00050"
     CDS             complement(2725..>3156)
                     /locus_tag="VFZ80_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX6285849.1"
                     /translation="AVVISKPARNVKAEDASQYIFGYTAANDVTARDLQRRDGQWTRA
                     KGFDTFCPLGPAIETELDPLERLAVICKVNGEVRQAGFTSDMVFGVAEILEYISAFTT
                     LLPGDVILTGTPSGVGKVEPGDVMEVEVDGIGTLTNRVVGT"
BASE COUNT          563 a         1015 c          992 g          586 t
ORIGIN      
        1 gacggcgatg tcgcctatca ggtgatcggg accggacccc tcgaccttcg ttgcaacgct
       61 gggcagactc gtcggcttcg acggtgtcga ccaccttggc gggagccgat acggtgatcc
      121 gatgcgagca cgagccactt cggcccatga gattccggca cgagagccaa ttcgactcga
      181 acccggtcac caggtcgagg ttggcgagcc tgatagccaa tggccggcgt ttgtcttcgt
      241 caccggtccg acggggcagg gttgggtgcc gtcacgccat ctctctgcgg atgcaggaac
      301 cgccgtggtc gtcaccccat atgacacgac ggagctgagc ctcgaagccg gcgaggtggt
      361 cgacgtcctc gatcgcgacg atgagagcgg atggtggtgg tgccgccgcg gggatggggc
      421 ccagggatgg gttccggttt ccgcactcga accggagaag tgaccatcgg ctgcgggcga
      481 cccctgccag tcacacggcc tccgttgcgc ctggggaaga agtggcagtc ccgaccggat
      541 tcgaaccggc gtttccggct tgaaaggccg gcgtccgagg ccactggacg acgggaccgg
      601 gtgcagacac gggaaggtta cgccgaggcc aaactcagcc caactctctc cgggaatctg
      661 gcctgtgcaa agggctcggg atgcgataga atcgaacaca tgttcgccac cgatggccag
      721 cacagcctct tcgctgcgga ggaggcttcc ttcgacccgg actacacgca gctgcgccgt
      781 atccaactcg atgacaccgc atggctcgac tacagcccga tgtggctcgc cggtgacgag
      841 acgctgtttc atctgctgac cgagcgagcc gagtggagac agcccgtggt gaggatgtac
      901 gaccgcgacg tcctcacacc caggctcgtg gcccgactcc agcccgacat acatcccgtt
      961 gtcccggaga tgatcgacag cctttcgtct cgctacggcg tgagactcga tcaggtcagt
     1021 gctggttggt atcgcgacgg gaacgactcc gtcgcgtatc acggcgaccg ggtcgctcgg
     1081 gaacgagaag aggcgatagt ggccaccgtc tcactgggtg ctgcccggcg cttcctgatc
     1141 aagcccaagg acaggggcgg ggaaactcac tccttctccc ttggtcacgg cgacctggtc
     1201 gtgatgggag gcagctgtca gaggacgtgg gagcatgccg tcccgaaggc ggcggcggcg
     1261 gggccgcgcc tcgcgctcat gttccgccac ttctacatct gaggtctcaa cccagcttcg
     1321 ctcgcgctct gtcgatccgt tccagcgttc tctccctgcc caacgccgag atcgactcga
     1381 acagcggagg gctcaccgag gacccggtga ccgccacccg catcggctga aaggtcttgc
     1441 cggccccgat acccagcttc tctggaattg cgcgcagagc cgactcgacc gacatggtgt
     1501 cccaggtgtc gagcccggcg atggccgacc ggccttcctc cagaacctcg accaccccct
     1561 ccttggtcat gaccttttcc caggactggt cgtcgtaagc gtcgaagtcc tcgaacaaga
     1621 atgtcacctg gctgcccgcc tccggaagaa gacgcgttcg ctcctgtacc agcggtgcga
     1681 tgtcaccgaa tttcgcccac tcttcagagt cgagagctct ccccacctcg gcctccacat
     1741 agggcctcac caactcggcg aaagcagtgg tgtccatggc acgtatgtac tcgccattca
     1801 tccacgccag cttctccggg tcgaaggccg cggggttctt ggagacatcg accagatcga
     1861 acttctcgac tgcctcctga cgatcgaaga tcgtcgtctc gccgtcgggt gaccatccga
     1921 gaatggccaa gtagttgaac atcgccgagg ggagaaaccc ctggtcccgg tactcggcca
     1981 acgccgtggc cccatgtctc ttggagagct tcttcccatc ggtgccaaag agaaggggta
     2041 ggtgggcata ggtcggctcg tctcccccga gggccctggt gagcaatatg tgtttgggag
     2101 tagagggcag caagtcctca ccacgggcga cgtgagttat ctcgtactcg acatcatcca
     2161 ctgtcgaagc caggtggtag gtgggcgtcc cgttgcttcg caggatgacg aagtcgtcga
     2221 tgtcgtttgg agcaaaagac aggtggcccc tgatcaagtc atcgaactcg acggcccggt
     2281 ccgggggaat ggacagtctt atgacgccgg agctcgtctc gcgttcgggg cgacggacgt
     2341 agtgacccgg atgctttccc tccttctggg ctcggtcacg gagggtgtcg agctcctcgg
     2401 tcgtgcgatc gtcgtagtag gcgtgacccg actcgacgag gcctcgagca agctcggcgt
     2461 agtgctcgat gcggtcggat tgtcggtagc tcccgtgcgg accccccact tcgacgccct
     2521 cgtcccagtc gagaccgagc caacggaacg atcccaggat ctcctcctca tactccttcg
     2581 tcgatcgctc gaggtcggtg tcgtcgatgc ggacgatgaa ggtgccgacc tcgtgcctgg
     2641 cgtagagcca gttgtacagg gctgcgcgcg ccgtaccgac atgcaggaat cctgttggcg
     2701 atggcgccat cctcacccgg acgctcacgt gcccaccacc ctgttggtca gggtgccgat
     2761 cccgtcgacc tcgacttcca tgacatcgcc gggctcgacc ttgcccactc ctgagggggt
     2821 accggtgaga atcacatccc cgggaagcag tgtggtgaaa gccgagatgt actccaggat
     2881 ctcggcgaca ccgaagacca tgtcggaggt gaacccggcc tggcgcacct ctccattgac
     2941 cttgcagatc accgccagcc gctccagcgg gtcgagctcc gtctcgatcg ccggacccag
     3001 cggacagaag gtgtcgaacc ctttggcacg ggtccactgc ccgtcgcgac gctgcagatc
     3061 gcgggcggtc acgtcgttgg ctgccgtgta gccgaagatg tactgggagg catcctccgc
     3121 tttgacgttg cgggcgggct tgctgatgac cacagc
//
LOCUS       DASUTA010000003         2304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_259550, whole genome shotgun sequence.
ACCESSION   DASUTA010000003 DASUTA010000000
VERSION     DASUTA010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259550"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1030
                     /locus_tag="VFZ80_00055"
     CDS             200..1030
                     /locus_tag="VFZ80_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX6285850.1"
                     /translation="MLTYHTPETATELVTLRAEHGSASLLMGGGTIVMTLLNEGVPLP
                     RIVIGMKRAGLDTISEDDGSKLIGAATTLATLTSDPHPMLSEAAASCGGWAIRNMATI
                     GGNLFVPAPSGDLGVALLALDASLRIDSVDGHRLVPIDDFYRGGRSLDEREVLTGIEV
                     PTTEKETRFIKFGRKHGPTPSVVTVAISLRLENGTVSESRIALGALGPHPVRARRAES
                     LLDGNELDPALVEEVAAAAADDFEGLTDAVASAWYRKRMARLHVKRLLEEVMSETHSG
                     "
     gene            1011..1493
                     /locus_tag="VFZ80_00060"
     CDS             1011..1493
                     /locus_tag="VFZ80_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6285851.1"
                     /translation="MKHTAVSFRLGEREIEVLVEPLTTLQSVLRDQLGYMATKDGCRQ
                     GGCGSCTVLVDGEPYLSCILPVEDVIDRRIDTVETQTPVDGLNPIQQALLDHNGLQCG
                     YCTPGFVMITTALLERQPHPDRDEIIEAIGGNYCRCTGYRPILDAISAVAGPISEEAQ
                     "
     gene            1490..>2304
                     /locus_tag="VFZ80_00065"
     CDS             1490..>2304
                     /locus_tag="VFZ80_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6285852.1"
                     /translation="MRELATVGRSLERVDGVGHVTGRSVFATDRWFRDLLHLKMVRSP
                     VPHALIRGVDLSEAEKVPGFVRALTHRDVPHNVYTVLSLIGVEPEDEFVLAEKRVRYI
                     GEPIVAIVAETERAAHEAVAKVRLDLEELPAVFDIEEALKPDAPVVTDWGDNVFHFEN
                     GHPCRRVRLGDVERGFSEADHIVEATYDTKPIEHAPTETTACIAQPQPDGRVTVYTNT
                     QALYFSLDNTSIILDIAPNRLQFVGGTVGGGFGGKVDVIVEPIAALAAIKTNRP"
BASE COUNT          418 a          773 c          718 g          395 t
ORIGIN      
        1 ggcgggacga agaagacggg ccgagacgaa accttcgatc tcggacatgg gtgggacgta
       61 tctggtggtg aagaagtcct ggaaccgtgc caccccgtcg gtgggcacgg agaacttgat
      121 gtgacgctcg atcactcggc ctccaattgg tcttacctgt tgaccaccat accaccgccc
      181 cgtatagtcg gtccaacccg tgctgaccta tcacacccca gagactgcga cggagctggt
      241 cactcttcgg gcagagcacg ggtcggcgtc cctcctcatg ggtggcggca ccatcgtcat
      301 gacgttgctg aacgagggtg tgccgcttcc ccgcatcgtg atcggcatga agcgggccgg
      361 tctcgacacc atctccgagg acgacggatc caagctcatc ggcgccgcca ccaccctggc
      421 cacactcacc tccgaccctc accccatgct ctccgaggcc gccgcttctt gtggggggtg
      481 ggcgatccga aacatggcca ccatcggcgg caacctcttc gtccctgctc cgtcgggaga
      541 cctcggtgtg gcccttctcg ccctcgacgc cagtcttcgg atcgacagtg tcgacgggca
      601 tcgactcgtg cccatcgacg atttctaccg gggcgggcgg tcactcgatg agcgcgaggt
      661 cctgaccgga atcgaggttc ccaccacgga gaaggagacc cggttcatca agttcgggcg
      721 caaacacggt cccactccat cggtggtgac ggtggcgatc agtcttcgcc tggagaacgg
      781 cacagtctcc gaatcacgca tcgcgctcgg tgccctcggc cctcatccgg ttcgcgcccg
      841 gcgggcggag tcgttgctcg acggaaacga gctcgacccg gcgctcgtcg aggaggttgc
      901 ggcggccgcg gcagatgact tcgagggtct gaccgatgcc gtcgccagcg cctggtaccg
      961 aaagcggatg gcgaggctcc acgtcaaacg tctcctcgag gaggtcatga gtgaaacaca
     1021 cagcggttag cttccggctg ggcgagcgcg agatcgaagt gctggtcgag ccgctgacca
     1081 cgctgcaatc ggtattgcgc gatcagcttg gctacatggc cacgaaggac ggctgccggc
     1141 agggaggatg cggctcctgc acggtcctcg tagatggtga gccctatctg tcgtgcatcc
     1201 tccccgtcga ggacgtcatc gatcgtcgca tcgacacagt ggagacccaa accccggtgg
     1261 atgggctcaa cccgatccag caggcgctgc tcgaccacaa cggtctgcag tgcggatatt
     1321 gcacccctgg gttcgtgatg atcaccaccg cgctgctcga gcgacagccc caccccgaca
     1381 gggacgagat catcgaggcc atcggaggca actactgccg ttgcaccgga taccggccga
     1441 tcctcgacgc catctcggcg gtggcaggcc cgatttcgga ggaagcgcag tgagggagct
     1501 ggccactgtc ggtcgctccc tggaacgggt cgacggtgtc ggccacgtca ccggacgcag
     1561 cgtgttcgca accgaccgct ggtttcgaga ccttctccat ctcaagatgg tccgcagccc
     1621 ggttccccac gctctgattc gcggggtcga tctgagtgag gcagagaagg tgcccgggtt
     1681 cgtgcgggca ttgacccacc gcgacgtccc ccacaacgtg tacaccgtgc tctccctcat
     1741 cggtgttgag cccgaggacg agttcgtcct cgccgagaag cgggtccgct acatcggtga
     1801 gccgatcgtg gcgatcgtgg ccgagaccga gcgtgccgcc cacgaggcag tggccaaggt
     1861 gaggctcgac ctcgaggaat tgcccgcggt cttcgacatc gaagaggcac tgaagcccga
     1921 cgctccagtc gtcaccgatt ggggcgacaa cgtgttccat ttcgagaacg gccacccctg
     1981 ccggcgagtg cgtctcggcg acgtggagcg cggcttttcc gaggccgatc acatcgtgga
     2041 ggcgacctac gacaccaagc ccatcgagca cgccccgacc gagaccacgg cgtgcatcgc
     2101 acagccgcaa cccgacggtc gcgtcaccgt ctacaccaac acccaggccc tctacttcag
     2161 tctggacaac acctcgatca tcctcgacat cgcccccaac cgactccagt tcgtcggcgg
     2221 gaccgttggc ggtggctttg ggggcaaggt cgatgtcatc gtcgagccga tcgccgcgct
     2281 ggccgcgatc aagaccaacc gacc
//
LOCUS       DASUTA010000004         2738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_777779, whole genome shotgun sequence.
ACCESSION   DASUTA010000004 DASUTA010000000
VERSION     DASUTA010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777779"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFZ80_00070"
     CDS             <1..249
                     /locus_tag="VFZ80_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6285853.1"
                     /translation="GFSAMVVDYSMPRSNGVELVRSMRAADVRIPIVMVSGVATQEEK
                     DAAWAVGVDAYLDKFDLRQGVLTKTLRQLIGLEEADTA"
     gene            358..1269
                     /locus_tag="VFZ80_00075"
     CDS             358..1269
                     /locus_tag="VFZ80_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6285854.1"
                     /translation="MPDLPRLDQLLMLLPAVIGLATVIWAWRITAAGREREREVRRLS
                     EEAHTIKNDFVALVSHELRTPLTSIAGFSDTLLQSWSDLPAEEIDEFLTIINRQALFL
                     GDLVEDVLVIPRLEANRLRLDPELFDLGDLIEDVAQMVFPNGGRKSSLVSLPDGVRVF
                     ADRRRVQQVLRNVMENARKYGGDQILVEGFVLGDQYLIIVSDNGPGVPDEETQKVFED
                     FEQLSKGDARDSSGMGLGLPIARRLARAMGGDVWYERRFPTGARFCYSLPLNRRTVLI
                     DEDGPELSPEEMVRVELPDAVRAFKGA"
     gene            1317..>2738
                     /locus_tag="VFZ80_00080"
     CDS             1317..>2738
                     /locus_tag="VFZ80_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX6285855.1"
                     /translation="MQQQTQWLVDDVPTESPLFAELNPTQREAVTATEGPVLVVAGAG
                     SGKTRVLTFRIAHLIRDMQVSPDSILAITFTNKAANEMKERVGQLVGGVVRTMWVSTF
                     HSSCVRILRREASRLGYKSGFTIYDDADSLRLLRMVIKDLDLDSKRFPERAMKAVISN
                     AKNELVDFESFADQGDGFYHEQVADIYRLYQQRLVEASAMDFDDLLMVTVELFAAFPD
                     VLAQYQHRFRYVLVDEYQDTNRAQYMLVKQLTAAHRNLCVVGDSDQSIYKFRGADIRN
                     IRDFEQDYPDARVIVLDQNYRSTETILDAANSVIANNTKRTPKHLWSDRGTGTSIVRF
                     EAEDEHDEAGYLVDEIEQLQKDGFNLADIAVFYRTNAMSRVIEDVFVRRQVPYSVVGS
                     VKFYDRKEVKDAVAYLRVVVNPADEVSVKRVINEPKRGIGNTTVAHVDRFSQAHGITF
                     LEALTRVSEIPQLNARAQNAVLEF"
BASE COUNT          526 a          854 c          817 g          541 t
ORIGIN      
        1 gggttcagcg ccatggtcgt cgactactcg atgccccgct ccaacggcgt cgaactggtc
       61 cgctcgatgc gggcggccga tgtgcgcata cccatcgtga tggtctccgg tgttgccacc
      121 caagaggaga aggacgcggc atgggccgtg ggagttgacg cctatctgga caagttcgat
      181 cttcgtcagg gtgtcctcac caagaccctt cgccagttga taggtctcga ggaagccgat
      241 accgcctgag agaacagctt ccgtcttctc tggccgtgca acctgggttg cgcatcgatc
      301 ctcgtactta atgcttaata cccgctatcc cgggtgccga tacgtcctat gtagaaaatg
      361 cccgatctgc cgcgtctcga ccagttgctg atgcttctgc cggccgtcat cggcctggcc
      421 actgtcatat gggcgtggag gatcacggct gcgggccggg agcgggagcg cgaggtgcga
      481 cgtctctccg aggaggccca caccataaag aacgacttcg ttgctttggt cagccacgag
      541 ctgaggactc cgctgacttc gattgccggc ttctctgaca ctcttctcca gtcttggtcg
      601 gatcttccgg cggaggagat cgacgagttc ctcacgatca tcaataggca ggctctgttc
      661 ctcggcgact tggtcgagga tgtgctggtc atcccccgcc tcgaggcgaa ccggcttcgg
      721 ctcgatccgg agctgttcga ccttggcgac ctgatcgaag acgtagccca gatggtgttc
      781 ccgaacggcg gtcggaagtc gtcgctggtt tctcttcccg acggtgtgcg ggtgtttgcc
      841 gaccgccgcc gagtgcagca ggtgctccga aatgtgatgg agaacgcaag gaagtacggg
      901 ggcgatcaga tcctcgttga ggggttcgtg ctcggcgacc agtacttgat catcgtctcg
      961 gacaacggac cgggcgtgcc cgacgaggag acacagaagg tcttcgagga cttcgaacag
     1021 ctctccaagg gcgacgcccg tgattcgagc ggcatggggc tcggtctgcc gatcgctcgt
     1081 cggctggcgc gagctatggg aggcgacgtc tggtacgagc ggcggttccc gaccggggcc
     1141 aggttctgct actcgctccc gctcaatcga cgcactgtcc tcatcgacga agatggtccg
     1201 gagttgagcc cggaggagat ggtccgtgtc gagctacccg atgccgttcg cgctttcaag
     1261 ggagcctgat cacacacgtg tgattcgtcc gtgacggccg gtaccatcgg tctcagatgc
     1321 aacagcagac gcagtggctg gtcgacgatg tccccacgga gtcgcccctg ttcgctgagc
     1381 tcaacccgac tcagcgcgag gcagtgactg caaccgaggg accggtcctc gtggtggctg
     1441 gagccggatc cggcaagacc cgggtgctca cattccgcat cgcccacctg atccgagaca
     1501 tgcaggtctc acccgattcc atcttggcga tcacattcac caacaaagcg gccaatgaga
     1561 tgaaggagcg tgttggccag ctcgtaggtg gtgtggttcg aacgatgtgg gtgagcacgt
     1621 ttcactcgtc gtgtgtccgc atccttcgcc gagaggcctc ccgcctcggc tacaagtccg
     1681 gcttcaccat ctacgacgac gccgactctc ttcggcttct cagaatggtc atcaaggacc
     1741 tcgatctcga ctcgaagcga ttccccgaga gggcgatgaa ggccgtcatc tccaacgcca
     1801 agaacgagct ggtcgacttc gaatctttcg ccgatcaggg cgacgggttc tatcacgagc
     1861 aggtcgccga catctatcgg ctctaccaac agcgtctcgt cgaagcgtcg gcgatggatt
     1921 tcgacgacct cctcatggtc accgtcgagc tgttcgccgc gttccccgac gtcctcgccc
     1981 aatatcagca tcgtttccgc tatgtcctgg tcgatgagta ccaggacacc aaccgagccc
     2041 agtacatgct ggtcaagcag ctcacagccg ctcaccgcaa cctctgcgtc gtcggtgact
     2101 ccgaccaatc gatctacaag ttccgcggcg ccgacatcag gaacatccgt gacttcgagc
     2161 aggattaccc cgatgcccgg gtgatcgtgc tcgatcagaa ctaccgatcc accgagacca
     2221 tcctcgatgc cgccaactcg gtgatcgcca acaacacgaa acgcacccct aagcatctct
     2281 ggtcagaccg ggggaccggg acttcgatag tgcgtttcga ggccgaggac gaacacgatg
     2341 aggccggcta tctggtcgat gagatcgagc aactccaaaa ggacggcttc aacctggccg
     2401 acatcgccgt cttctaccgc accaacgcga tgtcacgcgt catcgaagac gtcttcgtgc
     2461 gacgacaggt cccctactcg gtggtcgggt cggtcaagtt ctacgaccgt aaggaagtca
     2521 aggacgccgt tgcctatctg agggtcgtgg tcaacccggc ggacgaggtc tcggtcaaga
     2581 gggtgatcaa cgagccgaaa cggggcatcg gcaacaccac cgtggcccat gtcgaccggt
     2641 tcagccaggc ccacggcatc acattcctgg aggccctgac ccgggtctcg gagatcccgc
     2701 aactgaatgc ccgagcccag aacgcagtcc tcgagttc
//
LOCUS       DASUTA010000005         2820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1684970, whole genome shotgun sequence.
ACCESSION   DASUTA010000005 DASUTA010000000
VERSION     DASUTA010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684970"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /gene="tilS"
                     /locus_tag="VFZ80_00085"
     CDS             <1..851
                     /gene="tilS"
                     /locus_tag="VFZ80_00085"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX6285856.1"
                     /translation="ARTRLPPGAMTVALSGGADSAALAFLAARSGAGPALIHVHHGLA
                     ASDMLAAAASAIAQRLGLQMHTVSVDVPAGPSLEAQARDVRYEALATVEGAVVTGHTR
                     DDVVETVLINLVRGSGAEGLSGIPYHRPPNVYRPMLDLTRSETREIAALAELPFRDDP
                     SNDSLEMTRNRVRHRVLPVLRDLNPRVDEALARAADSLRADSDFIGHLANGPPAGELE
                     VSVIATAPRPVADRLVMRWLATQGVPVTAELLARVWAVVGGDSDRQDLEGGRSIVRDR
                     AVVRVE"
     gene            936..1457
                     /gene="hpt"
                     /locus_tag="VFZ80_00090"
     CDS             936..1457
                     /gene="hpt"
                     /locus_tag="VFZ80_00090"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX6285857.1"
                     /translation="MDIKPLITAEEIEAKLAEVGKQISEDYAGKEVLLVGVLRGAFMV
                     MADLARHIDLACEFDFMAVSSYGASTQTSGVVRILKDLDEEIEDRHVLIVEDIIDSGL
                     TLNYLIKSLRVRKPASLEIATLLLKEGIQRVPIDVRYVAFSIGPEFVVGYGLDYAGKY
                     RNLPYVGVMGEEA"
     gene            1589..>2820
                     /gene="ftsH"
                     /locus_tag="VFZ80_00095"
     CDS             1589..>2820
                     /gene="ftsH"
                     /locus_tag="VFZ80_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX6285858.1"
                     /translation="MSIILVAILFSWWFDQVAGPEEIRISEFVENVEDGKYSEVVLLA
                     RSKRAQARLEVGGNAEEGAFDQVASYVEGFESELTAILEQSGTDWSTDEEPAGFWELL
                     IGFLPWLFLLGFMVFIFMQMQGGGNRVMQFGKSRAKVVGKEQPKVTFDDVAGLQEAKE
                     ELEEIKDFLASPDRYRQMGAKIPKGVLLFGPPGTGKTLLARAVAGEAGVPFMSISGSD
                     FVEMFVGVGAARVRDLFEQAKQTAPAIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQL
                     LVELDGFEGNTGVIVMAATNRPDILDPALLRPGRFDRQITVDAPDVEGREAILAVHSK
                     GKPLGEDIDLAVLARRTPGFTGADLANLINEAALLTARKRNKQITMVELEEAIDRVIA
                     GPERKTRILSEDERKVIA"
BASE COUNT          489 a          917 c          909 g          505 t
ORIGIN      
        1 tcgcccgaac acggctcccg cccggagcga tgacggtcgc cctcagcgga ggtgcggatt
       61 cggcagcact agcgttcctg gcggcccggt cgggggccgg ccccgccctg attcacgtgc
      121 atcacgggct ggcggcatcc gacatgctcg ccgctgccgc ttcggcaatc gcgcaacgtc
      181 tcgggctcca gatgcacacg gtctccgtcg atgtgccggc cgggccttcg ctcgaggcgc
      241 aggcccggga tgtccgatac gaggccctgg cgactgtcga gggggcggtg gtcaccggcc
      301 atacccgtga cgacgtcgtc gagacggtgt tgatcaatct ggttcgaggc agtggcgccg
      361 aagggctgag cgggatcccg tatcaccgtc cgcccaatgt ctaccggccc atgctcgacc
      421 tgacacgttc agagactcgc gagatcgccg cactcgccga gctcccgttt cgcgatgacc
      481 ccagcaacga ctccctcgag atgactcgca accgggtcag gcatcgcgtc ctacccgtgt
      541 tgcgcgacct caacccgcgg gtggacgagg cgttggcccg ggccgcagat tcgcttcgcg
      601 ccgacagcga tttcatcggg cacctcgcca acgggccccc ggcgggcgag ctcgaggtca
      661 gcgtcatcgc cacagcccct cgtccggtgg ccgatcgcct cgtgatgcgt tggctggcga
      721 cccagggtgt tccggtgacg gctgagcttc tggctcgagt gtgggcagtc gtcggtggag
      781 attcggaccg ccaagacctc gagggtgggc gaagcatcgt tcgcgatcgt gcggtggttc
      841 gggtcgaata gcgacctgcg cggcgaagtc gctagctttg ccgactcgac gttccggggc
      901 gtcggggacg tctgctcaca cttcgaagga acgggatgga catcaagccg ctgatcacag
      961 ctgaggagat cgaggccaag ctcgccgagg tcggcaagca gatctccgag gactatgcgg
     1021 gcaaggaagt gctgctggtt ggtgtccttc gaggggcctt catggtcatg gccgacctgg
     1081 ccaggcatat cgacctcgcc tgtgagttcg acttcatggc ggtctcctcc tatggcgctt
     1141 cgactcagac ttcaggggtg gtccgcatcc tcaaggacct cgacgaggag atcgaggatc
     1201 gtcacgtgct gatcgtcgag gacatcatcg actcgggcct gactctcaac tatctgatca
     1261 agagtctcag ggtgcgcaag ccggcgtccc tggagatcgc caccctcctc ctgaaggagg
     1321 ggatccagcg ggttccaatc gatgtcagat atgtcgcctt ctccatcggc cctgagttcg
     1381 tcgtcgggta cgggctcgac tacgccggca agtaccgcaa cctgccctac gtcggggtca
     1441 tgggcgaaga agcttgaggc ctgtcccggg aagaatccgt cgagtgacgg ggttcgacta
     1501 ctggcccaac cggcgtagca tctaggtcaa ccctccccag aaccgaggtc ttgtgaaccg
     1561 gaccctgcga accctcatcg tctacgggat gtcgatcatc ctcgtcgcca tcctcttctc
     1621 gtggtggttc gaccaggtcg ccggccccga agagattcgc atctccgaat tcgtcgagaa
     1681 cgtcgaggac ggcaagtatt ccgaggtcgt gctcctggcc cggtcgaagc gggcccaggc
     1741 ccgcctcgaa gtgggaggca atgccgaaga gggagccttc gaccaggtcg ccagctatgt
     1801 cgagggcttc gagtccgagc tgaccgcgat cctcgagcag tccggcaccg attggtcgac
     1861 cgacgaggag cccgccggct tctgggagtt gctcatcggc ttcctgccgt ggctcttcct
     1921 gctcgggttc atggtcttca tcttcatgca gatgcagggt ggcgggaacc gtgtcatgca
     1981 gttcggcaag tcacgggcca aggtggtcgg caaagagcaa ccgaaggtca cctttgacga
     2041 tgtcgccggt ctccaagagg cgaaagagga actcgaagag atcaaggact tccttgcgag
     2101 ccctgacaga taccgccaga tgggcgccaa gatccccaaa ggcgtcctcc tcttcggccc
     2161 acccggcacc ggaaagactc tcctcgcccg cgccgtggcc ggcgaggcag gggttccgtt
     2221 catgtcgatc tccggatcgg acttcgtcga gatgttcgtc ggcgtcggtg ccgcccgtgt
     2281 ccgcgacctc ttcgagcagg cgaagcagac cgcaccggcg atcatcttca tagacgagat
     2341 cgacgccgtc ggccgccatc gcggtgccgg tctcggcggt ggccacgacg agagggagca
     2401 gaccttgaat cagctcctcg tcgagctcga cggtttcgaa ggcaacaccg gggtgatcgt
     2461 gatggctgcg accaaccgtc ctgacatcct cgaccctgcg cttcttcgcc cgggccgctt
     2521 cgaccgccag atcaccgtcg atgcccccga cgtcgagggg cgggaggcga tcctggccgt
     2581 tcattccaag gggaagcctc tcggggagga catcgatctc gccgtcctgg cccgtcgcac
     2641 ccccggcttc accggggccg acctggcaaa cctcatcaac gaggccgctc tgctcacggc
     2701 ccgcaagcga aacaagcaga tcaccatggt cgagctcgag gaggccatcg accgggtcat
     2761 cgccgggccg gagcgcaaga cccgcatcct ctccgaggac gagagaaagg tgatcgcata
//
LOCUS       DASUTA010000006         6480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1555998, whole genome shotgun sequence.
ACCESSION   DASUTA010000006 DASUTA010000000
VERSION     DASUTA010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6480
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555998"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VFZ80_00100"
     CDS             <1..645
                     /locus_tag="VFZ80_00100"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6285859.1"
                     /translation="PVTFLIDRAGVTGPDGPSHHGVFDLSYLRMIPNMVIGAPADATE
                     LCAMIETAVDHQGPIAIRYPKGGAVSVPSLPVAPLEVGRAQLHAEGDDVLLLAVGRMV
                     EVAQKAAVDLGEKGVSAGLVDARWVKPLDPRILEWAAPTPVVVTLEDNVIAGGFGAAV
                     MESFAAAGLTKEVLNVGVPDRFLPFGSAGDVMESVGMDSDSVVERVLLALGWGA"
     gene            complement(642..1475)
                     /locus_tag="VFZ80_00105"
     CDS             complement(642..1475)
                     /locus_tag="VFZ80_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6285860.1"
                     /translation="MTQHYDSEDELAPRPGGVYQFDAREFPWWLVAMLAIIAALVLAV
                     VFSEEYREAFSNIFPVPLRLAEGILMTFYLTFGSFLVALVLGLLIGLARVSKNAWLSN
                     VASTYIEFVRGIPMIVFIFLIALVVVPDFADLIGMESRSINQAIRGAAALSLFYAAFI
                     AEVFRAGIQSVPKEQSEAGRSLGLPDRQIMRRIVLPQAVRNMLPALGNDLISLMKDTS
                     LVSILAVREITQMARLYSGSTFRFRESFFILVIIYVVLTLILSLALRWYERRIAIPGR
                     I"
     gene            complement(1538..2482)
                     /locus_tag="VFZ80_00110"
     CDS             complement(1538..2482)
                     /locus_tag="VFZ80_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX6285861.1"
                     /translation="MENRNVWIGVGVAAVAVILLLIWSPWSDDAADETTTTTASAVTT
                     TTAAEETTTSAAEETTTTAGATSTTEAEAALPDLGGRTVTVGVENAYLPFNFILAGET
                     EGQGWDYDAWRDICERLNCVPEFVEAAWPAVIDQVAQGEFDTAADGISITDERAEIVD
                     YSIPYMVVQQKFIKQLGDDRFNSADDVINSDAIVATQVGTTNFELAADLVGEDRIQAF
                     DQFGLAIQALIAGDVDVVIIDDAAGLGYIGENADQIETIDDPLQSDPLGFIYPQGSDL
                     VEPVDLAIQAMIDDGTMEELGRTWFGPDFTVTYEDIEG"
     gene            2645..3772
                     /locus_tag="VFZ80_00115"
     CDS             2645..3772
                     /locus_tag="VFZ80_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6285862.1"
                     /translation="MKRYRLLVALAAMTLVFAACTEAADTTTTASGDDGATTAPGDDG
                     GTDGGGAGDTLAAVLDRGTLNCGVNDAVPGFGFTDAEGNFSGFDIDYCRAVAAAVLGD
                     PDAVEYTALTAQQRFTALQSGEIDVLIRNTTWTSSRDGTEGATFATTTFYDGQGMMVR
                     TDSGITEIEGLEGAAICVLSGTTTELNLATRMAGIDFEPLTFEDNETLQEAFVAGQCD
                     GWTSDKSQLAGVRSAFPEGPEALTILEETFSKEPLGPVTADGDTQWAQMVDWVVLATI
                     QAEEFGITSDNIDTFEDTEDLGIRRLLGLEFEDDEGNLTTFDPQLGFDRATWVQDVIS
                     AVGNYGEIFERNVGPDTPLGLERGLNALWTEGGLMYAPPYR"
     gene            3821..4999
                     /locus_tag="VFZ80_00120"
     CDS             3821..4999
                     /locus_tag="VFZ80_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6285863.1"
                     /translation="MAIADTRPTRSRPPFWRDERVLRVLIQVVAVAVIVAIGYVLWFN
                     LINNLQRQGISTGFELLDNPIGVQIAGSDLSPNASISRALLVGIKNTFALVVFGLPLL
                     TIIGVLVGVGRLSSNWLVAKASSIYVETLRNLPPLLVIFVMFFAIILPLPPARAPATP
                     LGLFVVSNLRISVPWLETTIDIGAFAVVLAVGALVAIGLWWWRTRRSDRTGEPHHRVM
                     WATVALVVIAVAGWVLLDRPVRGTVPRLDGRQVVGGFSGLGAYFAVLIALVLYTASHV
                     AEIVRGSILAVPKGQTEAANALALSGFQRLRFVVLPQAMRIAIPPTINQYLNFTKNTS
                     LAIAVGYAEVTLITFQAIGNGNPAPQLVLLLMAIYLMFSLTISAVVNFLNSRLSLETR
                     "
     gene            4996..6252
                     /locus_tag="VFZ80_00125"
     CDS             4996..6252
                     /locus_tag="VFZ80_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6285864.1"
                     /translation="MTLDTRPDELLGPPDEDRIDEALSPSEWVRAHLISSPLDTALTL
                     IFGVVFVAGAIWVVRYLLESDFTILRVNLTNLMVGRFPRDQVWRPAVALVMAGGAIGF
                     AGGLLRANARDRAREAGLPLETTGVLGLLRRFWPIAGFILLVLLLAQTVTPWLVVGAT
                     LGAGLLANLLSRAIPASVRKWGWAIVAGLFVGSYLVMVGGGVGYDSWGGLYLNIFLTV
                     AGITLAFPLGMLLALGRRSSLPALKVSSITYIEFIRGVPLITLLLLGAFALGFFLPDA
                     LQPSRTTRILIAIAMFEAAYIAEVVRGGFAAVPKGQVEAAQAVGLSPWKQTRLVVLPQ
                     ALRATIPAMVGQFISLFKDTTLVVIVALVDILGASQNANAQPEFFGQGLHRVTLPFVA
                     MAFWVGSYTMSREARRLERRLGVGER"
     gene            6249..>6480
                     /locus_tag="VFZ80_00130"
     CDS             6249..>6480
                     /locus_tag="VFZ80_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6285865.1"
                     /translation="MTAIDYADATRAKGEAIISLDSVDKFFGDFQALKDINVRVGKQE
                     VVVVIGPSGSGKSTLIRCINRLEKHDRGTIVVD"
BASE COUNT         1142 a         2032 c         2019 g         1287 t
ORIGIN      
        1 ccggtgacct tcctcatcga ccgggccggc gtcaccggcc ccgatggccc ctcccaccat
       61 ggcgtcttcg acctctccta ccttcgcatg attcccaaca tggtcattgg ggctccggcc
      121 gacgcaaccg aactgtgcgc catgatcgag accgccgtcg accatcaagg gccaatagcg
      181 atccgctatc ccaaaggtgg agccgtctcg gtcccctcac tgccggtcgc accactggaa
      241 gtaggcaggg cgcaactcca cgccgagggc gatgacgtgt tgctcttggc ggtgggacgc
      301 atggtcgagg ttgcccagaa ggctgccgtc gacctggggg agaagggggt ctccgcaggt
      361 ttggtcgatg cccgctgggt caagccactc gatccccgga tcctggagtg ggcggcgccg
      421 acgccggtgg tggtcactct cgaggacaac gtgattgccg gtgggttcgg tgccgctgtg
      481 atggagtcct tcgccgcggc cggattgacc aaggaagtgc tcaacgtggg tgttcctgac
      541 cgtttcctcc cgttcggttc tgcgggcgat gtcatggagt cggtcgggat ggactccgac
      601 tcggtggtgg aacgggtctt gctggccctc ggttggggcg cctagatccg ccccgggatg
      661 gcgatccggc gctcgtacca cctcagggcg agactcagga tcagcgtcag caccacgtag
      721 atgatgacca ggatgaagaa cgactctctg aaacggaaag tgctgcccga gtagagacgg
      781 gccatctgag tgatttctcg cacagccagg atcgatacca gagaagtgtc tttcatcaat
      841 gagatcagat cgttgccgag tgccggaagc atgttgcgca ccgcctgggg aagcacgatg
      901 cgcctcatga tctgacgatc gggcagtccc agcgaccggc ccgcttccga ctgctctttg
      961 ggcaccgact ggatcccggc gcggaatacc tcggcgatga atgcggcgta gaagaggctg
     1021 agggctgctg cccccctgat tgcctgattg atgcttcggc tctccatgcc tatgaggtcc
     1081 gcgaaatcgg ggaccacgac cagggctatc aagaagatga agacgatcat cggtattccg
     1141 cgaacgaact cgatgtaggt cgaagccacg ttgctcaacc aggcgttctt ggaaacccgt
     1201 gccaacccga tcaggagtcc caggaccaaa gcaacgagga acgagccgaa cgtgaggtag
     1261 aaggtcatca ggatcccctc ggcgagccga agcgggaccg ggaagatgtt cgagaaggcc
     1321 tcccggtact cctcgctgaa caccacagcc agaaccaggg cggcgatgat ggccagcatc
     1381 gcgaccagcc accaagggaa ctctctggcg tcgaactggt agaccccacc cgggcgcggc
     1441 gctagttcgt cttcgctgtc gtagtgttga gtcatgaaaa gggtgcgccg ggtttgatcc
     1501 cggcgcaccc tattccgtat cggtagctcg agtcggctta gccttcgatg tcctcgtagg
     1561 tgaccgtgaa gtcgggcccg aaccaagtgc ggccaagctc ttccatggtc ccgtcgtcga
     1621 tcatcgcctg gatggcgagg tccacgggct cgaccaggtc cgacccttgc gggtagatga
     1681 aaccgagcgg gtccgattga agcgggtcgt cgatggtctc gatctggtcc gcgttctcac
     1741 cgatgtagcc gagaccggcg gcgtcgtcga tgatcacgac gtcgacgtca cctgcgatca
     1801 acgcctgtat ggccagaccg aactgatcga acgcctggat gcgatcctct ccgacgagat
     1861 cggcggcgag ctcgaaattg gtggtaccga cctgggtggc cacgatggcg tccgagttga
     1921 tcacatcatc ggcgctgttg aacctgtcgt cgccgagctg cttgatgaac ttctgctgga
     1981 ccaccatgta ggggatcgaa tagtccacga tctccgctcg ttcatcggtg atcgagatgc
     2041 catcggcagc agtgtcgaac tcgccctggg ccacctggtc gatcacggcc ggccaggccg
     2101 cctcgacgaa ctcggggaca caattgaggc gctcgcagat gtcacgccag gcgtcgtagt
     2161 cccagccctg tccttcggtt tcaccggcca ggatgaagtt gaagggcagg taggcgttct
     2221 ccacgccgac cgtgacggtg cgaccaccga gatcgggcag agccgcctcc gcctcagtgg
     2281 tcgaggtcgc tcccgcagtc gtggttgtct cttcggccgc gctggtcgtc gtttcctcag
     2341 ctgcggtcgt ggtcgtgacc gctgaggcag ttgtcgtagt cgtctcatcg gcggcgtcat
     2401 ctgaccatgg gctccagatg agcagcagga tcactgccac tgctgcaacg ccgactccga
     2461 tccatacgtt tctattttcc atgcttttgg ggtccctcct gtcagaaccg acccggaagc
     2521 gtatatgcct cttccctttg gccgccattc ggacggctgc gcacgagcag gcgggcgatg
     2581 gctagggtcg caagcctgta cgccgatccc aggtcggcgc acaaccttga tagatggagg
     2641 atatatgaag agatacagac tcctggtggc cctcgccgcc atgacgctcg tattcgcggc
     2701 ctgtacggaa gccgccgaca ccacaaccac cgcatccggt gatgacggcg ccaccacagc
     2761 acccggggac gatggaggca cggacggagg cggggccggt gacacactgg ctgcggtgtt
     2821 ggaccgtggg actctcaact gtggtgtcaa cgacgccgtg cccgggttcg gcttcaccga
     2881 cgccgaagga aacttcagcg gcttcgatat cgactactgc cgcgccgtcg ctgccgccgt
     2941 tctcggcgac cccgacgccg tcgagtacac ggccttgacc gcccagcagc ggttcaccgc
     3001 cttgcagtct ggtgagatcg atgtgctgat acgcaacaca acctggacct cgtcacggga
     3061 cgggacggaa ggcgcgacct tcgcgacgac caccttctac gacggccagg ggatgatggt
     3121 gcgcaccgac tccggcatca ccgagatcga gggcttggaa ggtgcggcta tctgcgtgct
     3181 ctcgggcacc acgaccgagc tgaacctggc gacccgcatg gcgggaatcg acttcgagcc
     3241 attgaccttt gaggacaacg aaacgctgca ggaggcgttc gtggccggtc agtgtgacgg
     3301 ctggacttcg gacaagagcc agttggccgg ggtcagatcg gcatttcccg aagggcccga
     3361 ggccttgacg atcctggagg agaccttctc gaaggagccg ctcggtcccg ttaccgcgga
     3421 cggcgacacc cagtgggctc agatggtcga ttgggtcgtg ctggcgacga tccaggccga
     3481 ggagttcggt atcacgtcgg acaacatcga cacgttcgag gacaccgagg atctggggat
     3541 ccgtcgcctg ctcggtctcg agttcgaaga tgacgaaggc aatctgacca cgttcgaccc
     3601 gcagctcgga ttcgatcgag ccacctgggt gcaggacgtg atctccgctg tcggaaacta
     3661 cggggagatc ttcgagcgca acgtcggacc agatacgccg ctgggcctgg agcgcggact
     3721 caatgcgctc tggaccgaag gtggtctgat gtacgcacct ccttaccgct agtacgcagc
     3781 tacgtagggg gcgtctcacc aggcgccccc taccaacaag atggcgatcg cagacacccg
     3841 tccaaccagg tcacgtccgc cgttctggcg ggatgagagg gttctgcgcg tcctgatcca
     3901 ggtggtcgca gtcgcagtca tcgttgccat tggctacgtc ctctggttca acctcatcaa
     3961 caacctgcaa cgtcagggaa tcagcacagg gttcgagtta ctggacaacc cgataggcgt
     4021 tcagatcgcg ggcagcgatc tgagccccaa tgcctcgatc tcgcgagcat tgctggtcgg
     4081 gatcaagaac acttttgccc tcgtggtgtt cggccttccc ctcttgacga tcatcggggt
     4141 gctggtcggt gtggggagac tgtcttcgaa ctggctcgtt gccaaggcgt cttcgatcta
     4201 cgtggagacc ctccggaacc tgccaccact ccttgtgatc ttcgtgatgt tcttcgccat
     4261 catcttgcct cttccgccgg cgagggctcc tgccactccg ttggggctat tcgtggtcag
     4321 caacttgcgc atcagcgttc cctggctgga gacaacgatc gacatcggtg cctttgccgt
     4381 cgtcctcgcc gtcggcgcac tggtggcgat cggtctgtgg tggtggcgaa ctcgtcgatc
     4441 ggatcgaacg ggagagcctc atcaccgcgt gatgtgggcc actgtggccc ttgtcgtcat
     4501 agctgtggca ggatgggtgc tgctcgaccg acccgttcga ggaacggttc cacggctgga
     4561 tgggagacag gtggtcggtg gcttcagcgg actcggcgcc tacttcgccg tcctcatcgc
     4621 cctggtcctc tataccgcca gccacgtggc cgagatcgtt cgtggctcga tcctcgccgt
     4681 gcccaagggc cagaccgagg cggcgaacgc cctcgcgctc tccgggtttc agagactgcg
     4741 attcgtggtc cttccccaag cgatgcgcat cgcgatccca ccgacgatca accagtacct
     4801 caacttcacc aagaacacgt cactggcgat cgcggtcggg tacgccgaag tgaccctcat
     4861 cacgtttcag gcgatcggaa acggtaatcc agcaccccag ttggtgctgc tcctcatggc
     4921 catctacctg atgttctcat tgaccatctc ggcagtcgtc aacttcctca actccaggct
     4981 gagtctggag acgagatgac tctcgatacc cgccccgacg agttgctcgg cccccccgac
     5041 gaggaccgga tcgacgaggc cctgagccct tcggagtggg tccgcgctca cctcatctcc
     5101 agtcccctcg acaccgctct cactctcata ttcggcgtcg tcttcgtcgc cggagccatc
     5161 tgggtcgtca ggtatctgct cgagtcggat ttcacgattc tccgggtcaa cctcacgaac
     5221 ctgatggtcg ggcgatttcc acgcgatcag gtctggcgac ccgccgttgc attggtcatg
     5281 gccggagggg cgatagggtt cgccggaggc ctgctgaggg caaacgcccg cgatcgcgct
     5341 cgggaggcgg gcctgcccct ggagaccacc ggtgtgctcg ggctactgag gcggttctgg
     5401 cccattgccg ggttcatcct gctggtgctg ctcctcgccc agactgtcac cccctggttg
     5461 gtggtcggcg ccacgttggg cgctggtctg ttagccaacc tcctgagccg tgcgataccg
     5521 gcatcagtgc ggaagtgggg gtgggccatc gtggccgggc tcttcgtcgg ctcctacctg
     5581 gtgatggtcg gtgggggagt cgggtacgac tcttggggcg gcctctactt gaacatcttc
     5641 ctaaccgtgg ccggcatcac tctcgcattc ccgctgggga tgctcctcgc tctgggccgc
     5701 cgatcctcac tgccggccct caaggtctcg tcgatcacct acatcgagtt cattcgaggg
     5761 gtgccgctca taactcttct cttgcttgga gcgttcgctc ttggcttctt cttaccggat
     5821 gccctccagc ccagtcgcac cacccgcatc ctcatcgcga tcgccatgtt cgaggctgcc
     5881 tacatcgccg aagttgtccg gggaggattc gccgccgttc ccaagggtca ggtggaagct
     5941 gcgcaggccg tcgggctatc gccctggaaa cagacccgcc tcgtcgtcct gccccaagcc
     6001 ctgcgtgcga cgatccccgc catggtggga cagttcatca gtctgttcaa ggacaccacc
     6061 ctcgtggtca tcgttgccct ggtggacatt ctcggggctt ctcagaatgc caacgcccag
     6121 cccgagttct tcggacaggg gttgcataga gtgacccttc cgttcgtcgc catggcgttc
     6181 tgggtcggtt catacacgat gtcgagagag gctcgccgac tggagcgacg tctcggagtg
     6241 ggagagcgat gaccgcaatc gattacgcag acgccacacg cgccaagggg gaggcgatca
     6301 tcagcctcga ctccgtcgac aagttcttcg gagacttcca ggcgctgaag gacatcaatg
     6361 tgcgggtggg gaaacaggag gtggtcgtgg tcatcggccc gtccggctcg ggcaagtcga
     6421 cgctcatccg gtgcatcaac cgtctcgaga aacacgatcg aggcaccatc gtcgtcgacg
//
LOCUS       DASUTA010000007         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1685772, whole genome shotgun sequence.
ACCESSION   DASUTA010000007 DASUTA010000000
VERSION     DASUTA010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685772"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFZ80_00135"
     CDS             <1..265
                     /locus_tag="VFZ80_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285866.1"
                     /translation="ELPREADSLLSLARLRVEAIRVGLDEIVKLRDEIRLGPVELKPS
                     QEVRLQRLAPGSVLRAGEGTVFIPAKEPLVDNLIDFVKRMWEA"
     gene            329..1261
                     /locus_tag="VFZ80_00140"
     CDS             329..1261
                     /locus_tag="VFZ80_00140"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX6285867.1"
                     /translation="MKKLLSLVVLGAVALAACGGGSGAVAATVDGHDVTVGQVEALIE
                     SEGATIPKDQFAQFLGFQIQWHVINDAADADYGITVTEEEVAAEADRIVEEAAAEGQS
                     RDEFLAERGVTEEFLTNIARQGLIDSQIREILREDAPEPTGEDLEAQLNAAKAPLTNA
                     CVSHILVATEEEANDVLDRLEAGEEFGEIAMELSTDTGSAENNGILPCGTLETYVEPF
                     RDAALIAPIGEVYETPVESQFGWHVVMVTDRQDAPEADLPSGDQLADMVRDAAIVSEL
                     QAWFIGAVEEAEVTVEETYGTWQANPPQVVPPVG"
     gene            1288..>2163
                     /locus_tag="VFZ80_00145"
     CDS             1288..>2163
                     /locus_tag="VFZ80_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX6285868.1"
                     /translation="MITIVGLGPGALDRVPTSVRALLLDPGFVIVARTRHHPAAEDLA
                     GQRPVVFCDDLYEDGDRFDDVYEAIAQRVIDHSRDGSVIYAVPGSPMIGEFAVRKLLD
                     SGVDIEMIPGESFVDAVLAAVGYDPLDRGLQILNGHELPDPLILDKPTVIGHLDTPEV
                     LADVLDGVSRVLVEDAAVTVLAGLGADDATVVTEVPHEIDMALAGFRTSLFIDAEPGG
                     LTGAIRVMRRLRAECPWDREQTHQSLVKNLIEEAYELIEAIGRLPRRDVDWVAYAAVE
                     DELGDVLLQVLFHEAI"
BASE COUNT          404 a          686 c          688 g          385 t
ORIGIN      
        1 ggagctgcca cgggaggccg actcactcct ctcgctcgcc cggctgaggg tggaggcgat
       61 ccgcgttggt ctcgacgaga tcgtcaagct ccgcgatgag atcaggctcg gtccggtcga
      121 gctcaagccg tcccaggaag tcaggctgca gcggctggct ccagggtcgg tgctgcgggc
      181 cggagaaggc acagttttca taccggcgaa agagccactc gtcgacaatc tgatcgactt
      241 tgtaaagaga atgtgggagg cgtgaatctc acctagagag accgtccccc taccattttg
      301 gcagtcttcc ctgttcaaag gacttcccgt gaagaaactc ctatccctgg ttgtgctcgg
      361 agccgtggcg ctcgctgcat gcggtggcgg ctccggcgcc gtcgccgcca ccgtcgacgg
      421 gcacgatgtc accgtgggtc aggtcgaggc attgatcgag tcggaagggg ccaccatccc
      481 caaggaccaa ttcgctcaat tcctcggctt ccagatccag tggcacgtga tcaacgacgc
      541 cgccgacgcc gactacggga tcacggtcac cgaggaggaa gtagctgcag aagccgatcg
      601 aatcgtcgag gaggccgccg cggaaggtca gagtcgagac gagttcctcg ccgagcgggg
      661 agtgaccgag gaatttctca ccaacatcgc tcgccagggg ctgatcgaca gccaaatccg
      721 tgagatcctc cgtgaagacg cccccgagcc caccggagaa gacctcgagg cccagctcaa
      781 cgcggccaag gcgccactta cgaatgcttg tgtctctcac atcctggtgg cgacagagga
      841 agaggccaac gacgttctcg atcgtctcga ggcaggtgag gagtttggcg agatcgccat
      901 ggagctctcc accgacaccg gaagcgccga gaacaacgga atcctcccgt gtgggactct
      961 cgagacgtac gtggagccat tccgcgacgc tgcgctcatc gccccgatcg gtgaggtcta
     1021 tgagactccg gtcgagtcgc agttcggctg gcacgtcgtg atggtcaccg atcgccagga
     1081 cgcccccgag gccgaccttc cctcaggcga ccagttggcg gacatggtca gggacgcggc
     1141 gattgtctcc gagctgcagg cgtggttcat cggagctgtc gaagaagccg aagtgacggt
     1201 cgaagagacc tatggcacct ggcaggccaa ccctccccag gtggtacccc cggtcggcta
     1261 ggtgctggga gaatcggcac aacacagatg atcaccatcg tcggtctcgg tcccggtgcc
     1321 ctcgaccggg tgccgacgtc ggtgagagct cttctgctcg atcccggttt cgtcatcgtc
     1381 gcccgcaccc ggcaccaccc ggcggccgag gatctggcag gacagcgtcc agtggtcttc
     1441 tgcgacgatc tctatgagga cggcgatcgt ttcgatgacg tatacgaggc gatcgcccaa
     1501 agagtgatcg accactcccg tgacggctca gtgatctatg ccgttcccgg cagcccgatg
     1561 atcggcgagt tcgccgtccg caagctcctg gacagcggtg tcgacatcga gatgattccc
     1621 ggtgagtcat tcgtcgatgc cgtgctcgca gcagtcggct acgaccctct ggatcgcggc
     1681 ctccagatcc tcaacggaca tgagctcccc gatccgctga ttctcgacaa gccgaccgtg
     1741 atcggccatc tggacacccc ggaggtgctg gccgacgtgc tcgatggcgt gtcccgtgtc
     1801 ctcgtcgaag acgcggcggt gaccgtgctg gccggcctgg gcgccgacga cgccacggtc
     1861 gtcaccgagg tgcctcatga gatcgacatg gcactggccg gctttcgcac ctccctcttc
     1921 atcgacgccg agcccggcgg cctcaccggg gcgatacggg tgatgaggcg tctgcgcgcc
     1981 gagtgcccgt gggaccggga gcagacccac cagtctttgg tgaagaacct catcgaagag
     2041 gcctacgaat tgatcgaggc aatcggcaga ctccctcgcc gcgatgtcga ctgggtcgcc
     2101 tatgccgccg tggaggacga gctgggagac gttctgttgc aagtcctctt ccatgaggcc
     2161 ata
//
LOCUS       DASUTA010000008         2717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1685875, whole genome shotgun sequence.
ACCESSION   DASUTA010000008 DASUTA010000000
VERSION     DASUTA010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685875"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VFZ80_00150"
     CDS             complement(<1..278)
                     /locus_tag="VFZ80_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285869.1"
                     /translation="MRRRVLIGADLEDRYLVPLDARKAVGVAFGESFSWFGWMTVLYT
                     SLGALAFVAGIWLGWDALTPAGRLVYALAIGGLTVASLLVGWWWFIRG"
     gene            complement(260..496)
                     /locus_tag="VFZ80_00155"
     CDS             complement(260..496)
                     /locus_tag="VFZ80_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285870.1"
                     /translation="MTRYYHDRFFADTRYTAFAVLTLSLIGFWGVAEAFLLIPVVALL
                     GANQTAFDASYLFMADVTPPPSKEKSTGRCVVGC"
     gene            complement(636..711)
                     /locus_tag="VFZ80_00160"
     tRNA            complement(636..711)
                     /locus_tag="VFZ80_00160"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(676..678),aa:Thr,seq:tgt)
     gene            complement(754..1476)
                     /locus_tag="VFZ80_00165"
     CDS             complement(754..1476)
                     /locus_tag="VFZ80_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX6285871.1"
                     /translation="MAPAGYGSRVEGVHAVAAALAAGRVDRLFVERGRRDRLRELLAG
                     VDGDVIRLVDDVRSLADTDAPQGVVAECRPIDPVTLEHLSEGHPAALVLDHVEDPHNV
                     GAAARSAHAAGITGMVVSARRAAPLSAAAFKAAAGALETLPVSIVNSIPEALSRLKDN
                     QVWVVGLDAAGDRSLFGLDLLTEPVAVVVGSEGSGLAELTRKRCDLLARIPMAAGTES
                     LNASVTAALASFEIMRVRERVQ"
     gene            complement(1451..>2717)
                     /gene="cysS"
                     /locus_tag="VFZ80_00170"
     CDS             complement(1451..>2717)
                     /gene="cysS"
                     /locus_tag="VFZ80_00170"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX6285872.1"
                     /translation="TVQSEPHVGHGRYAVAFDVIRRYLTWRGYAVTYVSNITDVEDKI
                     IAAAEEAGAPVEEHAILMTKRFTDSFRRLGVLAPDLEPKASEHIPEMLELIAKLIDRG
                     LAYERRGDVYFSVKTLESYGRLSGRKPDELRAGYRIEVDEEKEDPLDFALWKAAKPGE
                     PTWDSPWGPGRPGWHIECSAMAAKYLGDGFDIHGGGLDLVFPHHENEVAQSEGASGET
                     FARYWLHNGMVNLGGEKMAKSTGRIVDLESIIDSHGGRALRLLYVRAHYRSPIDFSEE
                     LLDDAAESLDRLDRFRQRVEPGVPHPDAIERFREVMDDDFGTPQALSQVFDLVREGNR
                     LMDEGGDASSLAGAVAEIVDVLGIYDAEPAPHQLSQLSDLEIAELVEARDEARREKRY
                     GDADAIRDRLDAAGVMIEDGPHGTRWIRK"
BASE COUNT          508 a          899 c          848 g          462 t
ORIGIN      
        1 tcgcccctga tgaaccacca ccagccgacg aggagggagg caacggtcag ccccccgatg
       61 gccagtgcgt aaacgagacg tcccgctggt gtgagcgcgt cccagcccag ccagatgccg
      121 gccacgaagg cgagagcgcc cagggacgtg tagagaacgg tcatccaccc aaaccaggag
      181 aagctctcgc cgaatgcgac gccgaccgcc tttctggcgt ccagcggcac gaggtagcgg
      241 tcttcgaggt cggcgcctat cagcacccga cgacgcatcg ccctgttgat ttctccttcg
      301 agggcggcgg cgtaacgtcg gccatgaaca ggtaggaggc gtcgaaggcg gtctggttcg
      361 ccccgagaag agcgacgacc gggatgagca ggaaagcctc ggcgactccc cagaacccga
      421 tcagagacaa ggtcagcacg gcgaaggccg tgtacctggt gtccgcgaag aaccggtcgt
      481 ggtaatagcg ggtcatgccc cgcatccgtc gcagttgttc gtgggcgagc tcgagttcgt
      541 ggatgacagc cctccttcac ccaccgttcg gagcaaccat cacccttgga tggtgtgggc
      601 tccaagtagt tggggctcgg tcaggaggtt gcagttggag ccgcgtgccg gagtcgaacc
      661 gacgacctgc tgattacaaa tcagctgctc taccaactga gctaacgcgg cctggagctc
      721 tgagcgtgtg cagtaaccat gaccacgtca tggttactgc acacgctcgc gcaccctcat
      781 tatctcaaac gaggccaggg cagcggtaac cgaggcgttg agactctccg ttcccgccgc
      841 catcgggatt ctggctagaa gatcgcagcg tttgcgggtc aattcggcca gacccgaccc
      901 ttcagacccg acgaccacgg caaccggctc ggtgaggagg tcgagaccga acaggctccg
      961 gtccccggca gcgtcgagcc caaccaccca cacctggttg tccttgagac gactcaatgc
     1021 ctcggggatc gagttcacga tggagaccgg caatgtctcc aaggctccgg cagcagcctt
     1081 gaaagcagca gccgaaagcg gggcggcgcg gcgggcagac accaccatgc cagtgatgcc
     1141 ggccgcatgt gcgctcctcg ccgccgcccc cacgttgtgc ggatcctcga cgtggtcgag
     1201 aacgagagcg gcgggatgac cctcggacag gtgttccaag gtcaccggat cgatagggcg
     1261 gcactcagcc accacgcctt gtggcgcgtc cgtgtcagcc aatgacctca cgtcgtcgac
     1321 cagccgtatg acgtcgccgt cgacacccgc caggagctca cggaggcgat ctcgtcggcc
     1381 ccgctcgacg aatagtctgt caacccgtcc cgccgccagc gcggcggcca cggcgtggac
     1441 gccttcaacc ctacttccgt atccagcggg tgccatgggg cccgtcctcg atcatcaccc
     1501 cggctgcgtc gagacggtcc cgtatcgcgt cggcatcgcc ataccgcttc tctcggcggg
     1561 cttcgtctct ggcctcgacc agctcagcga tctccaaatc gctcaactgg gaaagctggt
     1621 gaggggccgg ctccgcgtcg tagatgccga ggacatcgac gatctcggcg acggctccag
     1681 caaggctcga ggcgtcaccg ccttcatcca tcagtcggtt gccctcccgt accagatcaa
     1741 agacctgtga cagcgcctgg ggcgtcccga agtcatcgtc catgacctca cgaaagcgct
     1801 cgatcgcatc tgggtgaggc acaccgggct ccacccgctg acggaaacgg tcgaggcggt
     1861 cgagtgactc ggcggcatca tcgagaagct cctccgaaaa gtcgatcggt gatcggtaat
     1921 gcgccctcac gtagaggagt cgaagagccc gcccgccgtg ggagtcgatg atcgactcga
     1981 ggtccacgat gcggcccgtc gacttcgcca tcttctcacc cccgaggttg accatgccgt
     2041 tgtggagcca gtaccgggca aaagtctcac cggacgcgcc ctcgctctga gcgacctcgt
     2101 tctcatggtg cgggaagacg agatcgagac cgccaccatg gatgtcgaag ccatctccta
     2161 ggtacttggc ggccatcgcc gagcactcga tgtgccagcc tggcctgccc ggcccccaag
     2221 gcgaatccca ggtgggctca ccgggcttgg ccgctttcca gagggcgaag tcgagtggat
     2281 cctccttctc ctcgtcgacc tcgattcggt agccggcgcg aagctcatcg ggcttccgac
     2341 cggagagccg gccgtaggac tcgagggtct tcaccgagaa gtagacgtcg ccgcgtcgct
     2401 cgtaggcaag gccgcggtcg atcagcttgg cgatcagttc gagcatctcg ggtatgtgct
     2461 cgcttgcctt gggctcgagg tcaggtgcca ggactccgag acggcggaac gagtcggtga
     2521 agcgtttggt catcaggatg gcgtgctctt cgaccggcgc acccgcctcc tcggctgcgg
     2581 cgatgatctt gtcctcgaca tctgtgatat tcgacacgta ggtcaccgcg taaccacgcc
     2641 acgtcagata gcgcctgatc acgtcgaagg ccaccgcgta ccggccgtga cccacgtgtg
     2701 gctcggactg gaccgtc
//
LOCUS       DASUTA010000009         3768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1815336, whole genome shotgun sequence.
ACCESSION   DASUTA010000009 DASUTA010000000
VERSION     DASUTA010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815336"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VFZ80_00175"
     CDS             complement(<1..853)
                     /locus_tag="VFZ80_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX6285873.1"
                     /translation="MRYPVHIDRAVFVPMDDGARISLTLYRPAAPGDGPFPTIVESLP
                     YRKDDEFMAEDYSTYSYLAERGFAGVRIDIRGTGASTGIIVDEYVEREQRDTLAVFDW
                     LESQSWCDGNLGMWGVSWGGFSALQTAMLRPKQLRAIAPMHATHDRFACDVHYTGGSA
                     HAAEQLDWPGSMVASNGLPPDPDVFGEGWFEEWMRRLEGTPQWIFEWLKHQRRDAYWL
                     HGSPCSDYSAIQCPTLLVGGWLDGYVEGMLALARHLECETRTIIGPWGHYRPATGVPE
                     PTLDHLDL"
     gene            1183..1656
                     /locus_tag="VFZ80_00180"
     CDS             1183..1656
                     /locus_tag="VFZ80_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6285874.1"
                     /translation="MSGKLRYEIDLSKPVPTPRSDGFRIRAVVPTDLAGLARLMLDAY
                     VGTIDYHGEDLDDATAEVRSFLDDPDSLLDRSYIVEDEGEVVSAVLVSMSQHMPFIGY
                     VMTLPSHKKQGLAHLVVTHALRRLADDGHQRVVLFITDGNTPSEKLFRSVGAVHL"
     gene            complement(1719..2654)
                     /locus_tag="VFZ80_00185"
     CDS             complement(1719..2654)
                     /locus_tag="VFZ80_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6285875.1"
                     /translation="MTYRGDLMRIGLINQLNGRPDGERPAPSWASVSERARLAEAVGF
                     DMFVFEDALLYRGKEHTNGLWESVSIAGALAATTDKMRIGQSVVNSPYRSPAMMASIA
                     TTLDEISGGRYILGIGAGNTEDSDYEAFGFPTDRRYSRFAEAIQIIHSLLKTGTADFT
                     GEFYTVKESELVLRGPSPQGPAINIAAGGEKMMTLVARYGDQWNWWGWEETIPQIEER
                     FQPIFENLERACESEGRDPGSLVRTFDLYSVLAPGLDADHEMTQPVAGSPQEIAEYAL
                     SLGELGFEEVRCDVWPKTDEAVAAMEPVVELVHRG"
     gene            2720..3133
                     /locus_tag="VFZ80_00190"
     CDS             2720..3133
                     /locus_tag="VFZ80_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009359289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6285876.1"
                     /translation="MEFTIHPVLPAHDINRAAHWYRDKLGLEPVKHGGEPISPGAHPE
                     RYEEELMYDTGTARWNVYESAHAGHNRATAARLVTPDFDAVFGELKSRGVVFEEYDFG
                     DDFRTVDGVLVSPDGEKTCWFKDSEGNILALGSSD"
     gene            complement(3148..>3768)
                     /locus_tag="VFZ80_00195"
     CDS             complement(3148..>3768)
                     /locus_tag="VFZ80_00195"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6285877.1"
                     /translation="EVRMGVPVLGVSQDFDRIEVATDLDVLRADGVVVAVPMPVLARP
                     GFLVEAPSSLLEALTRLRMGTAAKVAVATADEPPMFRRQEADYPIWYWTGARPGGSTR
                     RAVTGFGGTARGAARLVASAEERIARAVPETSLTGDAIVVDWGADPWTGGCYSALGPG
                     QRPLLSQLQEPWGRVVFAGEHVNGSGTIDGAIRSGEAAALSLLERV"
BASE COUNT          685 a         1211 c         1156 g          716 t
ORIGIN      
        1 gaagatccaa gtggtcgagc gtcggctcgg ggacgccggt tgccggtcgg tagtggcccc
       61 agggcccgat gatcgtcctc gtctcacact cgaggtgcct ggcgagggcg agcattccct
      121 ccacatagcc atcgagccaa ccgcccacga gcagtgtcgg gcactggatc gccgagtagt
      181 cgctgcaagg tgagccatgg agccagtagg cgtcgcgtcg ctggtgcttc agccactcga
      241 agatccattg cggagtgccc tcgagccgcc gcatccactc ctcgaaccac ccctcgccga
      301 agacgtcggg gtcgggtggc agtccgttgc tggccaccat cgacccgggc cagtcgagct
      361 gctctgcggc atgagccgat ccacctgtgt aatggacgtc gcatgcgaag cgatcatgcg
      421 ttgcgtgcat cggggcgatc gctcgcagtt gcttcgggcg aagcattgcg gtctggagtg
      481 cgctgaaccc accccacgag accccccaca tgccgaggtt tccgtcacac caactctggg
      541 actcgagcca gtcgaataca gccagcgtgt ccctctgctc ccgctccacg tactcgtcga
      601 cgatgatccc cgtcgaggcg cccgttccgc ggatgtcgat ccggacgccg gcgaatcccc
      661 gctccgctag gtagctgtag gtgctgtagt cctccgccat gaactcgtcg tccttgcggt
      721 agggaagcga ctccacgatg gtggggaaag ggccatcgcc gggtgcagcg gggcggtaaa
      781 gggtcaggga gatgcgcgct ccgtcatcca tcggcacgaa gaccgctctg tcgatgtgaa
      841 ccggatatct cacaatgtct caactgccat catcccactt ctggccgctt cgatactccg
      901 ccggttatcg atgctcgcca gggttcagga ttcagcggca gtttcgctga ttgaagtgtc
      961 caaacggttt ggagattctt ctgccatgga tggcgtctca tttcacatgg tcgacgggga
     1021 gttcttctcg ctgcccggtc cctcgggctc tggacaggac tgcatcgcga ggaagagagt
     1081 gcggtatcca cgaccttctc cgccgaggtg aagcatggct gatggccaac ctggagtcgg
     1141 ggggtgaggt cgccgagcag gatcatcgga tacgctggct acttgtccgg gaaactgaga
     1201 tacgagatcg acctgtcgaa gccggtcccg accccgcgat cagatggctt tcgtatcagg
     1261 gccgttgtcc ccaccgatct tgcaggcctt gccaggctga tgctcgatgc ctacgtcggc
     1321 accatcgact accacggcga ggacctcgat gacgccacgg ctgaggtccg gtcgtttctg
     1381 gatgatcccg attcgttact cgaccggtca tacatagtgg aagacgaggg ggaggtcgtc
     1441 tccgcagttc tcgtttcgat gtcgcaacac atgccattca tcggctacgt gatgactctc
     1501 ccctcacaca agaagcaggg tctggcgcat ctcgtcgtca ctcatgcctt gagacgactt
     1561 gccgatgatg gccaccagag agtcgtcctc ttcatcaccg acggaaacac cccctcagag
     1621 aagctctttc gctccgtcgg tgccgtccac ttgtgagcgt gtgcagatac cgtgccgtgg
     1681 ccaccgtatc tgcacacgct cgcaacaggt cctgctcgtt atccccggtg taccagttcg
     1741 acgacaggtt ccattgcggc gacagcctca tcggtcttcg gccatacgtc acagcgaacc
     1801 tcttcgaagc cgagttcgcc tagagaaagg gcgtattcag ctatctcctg tgggctgccg
     1861 gccactggct gtgtcatctc atggtcggcg tcgagaccgg gagccaggac gctgtacagg
     1921 tcaaaggtgc gaaccagact gcccgggtcg cggccttcac tctcacaggc tcgctccaga
     1981 ttctcgaaga tgggctggaa acgctcctcg atctggggga tggtctcttc ccatccccac
     2041 caattccact ggtcgccgta gcgggcgacc agggtcatca tcttctcccc gccggcggcg
     2101 atgttgatcg ccggtccctg tgggctgggg cctcgaagca ccaactcgga ttccttcacg
     2161 gtgtagaact caccggtgaa gtcggctgtg ccggtcttga gcaggctgtg aatgatctgg
     2221 attgcctcgg cgaatctcga ataccgtcgg tcggtgggaa agccgaaggc ctcatagtcc
     2281 gagtcctcgg tgtttcccgc accgataccg aggatgtaac gacccccaga gatctcatcg
     2341 agggtggtgg cgatactggc catcatcgcc ggagaccgat agggcgagtt caccaccgac
     2401 tgcccgatcc gcatcttgtc ggttgtcgcc gccagggcgc cagcaatcga caccgactcc
     2461 cagagcccat tggtgtgctc cttgccgcga tagagaaggg cgtcttcgaa gacgaacatg
     2521 tcgaatccga ccgcctccgc cagtcgcgcc ctttcagaca ccgaagccca tgacggtgca
     2581 gggcgttccc catcggggcg gccgttcagt tgattgatga gccctatacg catcagatcg
     2641 ccacgatacg tcatgcggcc gcgatcgcat gtcgagaaac tctgtccgac cgggcgattc
     2701 tccgcgttac gttgcccgga tggaattcac gattcatccg gtgctgcccg ctcacgacat
     2761 caacagggcg gcccattggt accgggacaa gctcggtctg gagcctgtca agcatggcgg
     2821 tgagccgatc tcgccgggtg cgcaccccga gaggtacgaa gaggagctga tgtatgacac
     2881 cggtaccgca cgatggaatg tctacgagtc tgcgcatgcg ggccacaacc gggcgaccgc
     2941 ggcgcgtctg gtcactccgg acttcgatgc cgtcttcggg gagttgaagt cgaggggagt
     3001 tgtcttcgag gagtacgact tcggcgacga cttccgcacc gtcgacggtg tgctggtctc
     3061 accggacggg gagaagacat gttggttcaa ggactccgag ggcaacatcc tcgccctggg
     3121 ctcatccgat tgacgctccc gagacgatca caccctctcc agcaggctca gggcagccgc
     3181 ctccccgctg cggatggcgc cgtcgatggt cccactgccg ttgacgtgct cgccggcgaa
     3241 cacgacacga ccccatggct cctggagctg gctcaacaac ggtcgctgac cggggccgag
     3301 agccgaataa cagcctccag tccacgggtc ggccccccag tccacgacga tggcatcgcc
     3361 cgtcaacgag gtctcgggca ccgcccgagc gattcgttcc tcagccgatg ccacgagtcg
     3421 ggccgcaccc cgggcggtgc cgccaaaacc ggtgactgcc ctccgggtcg acccgccggg
     3481 tctcgctccg gtccagtacc agatcgggta gtcggcctcc tggcggcgaa acatgggcgg
     3541 ctcgtctgcg gtggccaccg ccaccttggc cgcggtgccc atccgcaggc gagtgagggc
     3601 ttcgagcaaa gatgaaggtg cttcgacgag aaaaccgggc cgggccagaa ccggcatggg
     3661 cacggcgacg acgacgccat cggcccgaag gacgtcgaga tcggtggcga cctcgatccg
     3721 atcgaagtct tgggagacac cgagcacagg gacgcccatt cgcacttc
//
LOCUS       DASUTA010000010         3392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1815710, whole genome shotgun sequence.
ACCESSION   DASUTA010000010 DASUTA010000000
VERSION     DASUTA010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815710"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFZ80_00200"
     CDS             complement(<1..329)
                     /locus_tag="VFZ80_00200"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HEX6285878.1"
                     /translation="MEKVYEGIGIKVHRVGPICQHAGFCANQTTDWFQMLPDTDDITV
                     KGRVIDMIEHCPSGALVCEIDDEIIEPDYPKAISPVEDGPYWVTGGVTIERSDGVTLE
                     VRNRVTL"
     gene            complement(1262..2923)
                     /locus_tag="VFZ80_00205"
     CDS             complement(1262..2923)
                     /locus_tag="VFZ80_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285879.1"
                     /translation="MATTGHASPNADTVAFTAQSEPTVLSYFEAFSSRPSWRELARWP
                     PDVFALTNLILDHTEAYRFTVAPPRGARWPPRGDWNERVQRAADEWRVTAANPQRSPP
                     SDVVKLWRVVLERRDTPLSVLRRGEDCPLLRALLTLHAIADEACRGLASSQPAPAASF
                     ERAAWDLLSARGSLAHIEPTRVRITPKTQLASRGITIRSLSRYLALNYESIDVHWRRI
                     DPLPDRPERTKRDYNILLVPWPLQVTDSAFRPVEGPLENMDPGTFGFFQYDPDESLDV
                     RLLRTLVEQAQQRVKRIDTVITPEASIRAEEIPALEMLMTELGVLSVIAGVRQSPRRG
                     KLGRNYVHLGVRTGEGWTTFEQEKHHRWCLDQTQIRQYHLSHALDPSRQWWEAIDLPA
                     RTVEIIDLGGGATVAPLVCEDLARMDEVSDLLRRIGPTLVVALLLDGPQLPQRWPCRY
                     AAVLADEPGSAVLTLSAFGMVARSRPSGKPRSRVVAMWSDPMSGPQHIELGRGASGIL
                     LTASAEAKTVWTADGRRHDTTPSLTLRNVEQMRVKRSPSQRRQVA"
BASE COUNT          603 a          997 c         1089 g          703 t
ORIGIN      
        1 cagagggtga cccggttgcg cacttccagg gttacgccat cgctgcgttc gatcgtcacg
       61 ccacccgtga cccagtaggg gccgtcttcc accggcgaga tcgccttcgg gtagtcgggt
      121 tcgatgatct catcgtcgat ctcacagacc agcgcacccg aaggacagtg ttcgatcatg
      181 tcgatgaccc gacccttcac ggtgatgtcg tctgtatcgg gcaacatctg gaaccagtcc
      241 gtggtctggt tggcgcagaa acccgcgtgc tgacagatag gcccaacccg atggaccttg
      301 atcccaatac cctcgtaaac cttctccacg gcagagaagg tctcggtcga ggcgacctcg
      361 gtgccgtcga aaccaacctt gaggtgagtt ctgtcacaga aaggcttgtg tgacgattgg
      421 ccgcatcggc atagccacgt gggagtgtcg tgggggagcg cctcatcagt cgcccaggtc
      481 agcggatcgc cttcaggaga tcggatgata cgtttcggcg tgatagggag atcagcgttg
      541 atctgatatg gaccgttggt ggtgatcttg atgtcaggct caggcacgaa ctcgcctcat
      601 ccgcatcgag gctactcgct gtcgcatttc catcgtcccg ccaatcggac ctgatcgcag
      661 gtcactgcgc ccatctgtgg agtctccgat ccaacgtcgc gcagcttgca gttctcagcg
      721 tcgagctaga gaccgttgga ggcaccaaca tcctctctaa tgcacctatt tcggggcgcg
      781 ttcgacgtgg tcccacaaaa gccctgctcg gcgggcggtt cacctggttc atgaagaggg
      841 tgtgttgtgg ggcgttgtga aagaaggcgc gatcatcagc ttgaaccctt ctaagactga
      901 caaacggtgg ccacgcccgc tcagcgcgtc tggatggatt ccctcgggcg ccgttatgaa
      961 tatgtcccag actgcgtcga cgtcccgatc gttgccatga cactggcttc agacatagtt
     1021 gaaacggtat cggttcgccg gtggctgcga tctcgttgcc tgctggctgc gggcggccgc
     1081 ccggcatcgt tcgacgccgg gagctgagtc aggccgtatc aatcgggagt atttgaaatc
     1141 ggcttggaac cctttgcgga cctgacctgg cggaccaaga acggtgcact tggtccggcc
     1201 accgtgggtg gtgtcgatgt cgataccagc cgacgaggca tcaggacgtg gccgcctcag
     1261 gtcaggccac ttgtctgcgc tgggatgggg accgcttgac tctcatctgc tctacattgc
     1321 gaagtgttag tgatggagtg gtgtcatggc gtctgccgtc ggctgtccac accgtcttgg
     1381 cctctgcaga ggctgtgagg agtatcccgc tcgccccccg tccgagctcg atgtgttggg
     1441 ggccgctcat ggggtcgctc cacatcgcta cgactctcga tctcggtttc ccgctgggtc
     1501 gggaccttgc caccatcccg aatgccgaga gcgtgaggac ggccgatccg ggttcgtcgg
     1561 cgaggacggc ggcgtagcgg cagggccaac gttgcgggag ttggggcccg tcgagaagga
     1621 gggccaccac cagagtcggc ccgatccggc ggagcaggtc tgatacctcg tccatccgtg
     1681 ccaggtcctc acacaccagt ggggctacgg ttgcgccacc tccgaggtcg atgatctcaa
     1741 cggttcgggc cggtaggtcg atggcttccc accattgtcg ggaggggtcg agggcgtggc
     1801 tcaggtggta ttgacggatc tgggtctggt cgaggcacca tctgtggtgt ttctcctgtt
     1861 caaaggtcgt ccagccctcg ccggtgcgaa caccgaggtg cacgtagttc cgtcctagct
     1921 tccctcgcct cggggactgc cggacaccgg cgatgacgga gagcacaccg agttcggtca
     1981 tgagcatctc gagcgccggg atctcctcgg cacgaatgga tgcctcgggc gtaatcacgg
     2041 tgtcgatccg ctttacccgt tgttgggctt gttcgacgag cgtccgcaac agcctgacat
     2101 cgaggctttc atccgggtcg tactggaaga agccgaaagt gccgggatcc atgttttcga
     2161 gaggtccttc caccgggcga aaggcgctgt ccgtgacctg gaggggccac ggcaccagga
     2221 ggatgttgta gtccctcttg gttcgctcgg ggcggtcggg gagggggtcg atgcggcgcc
     2281 agtgaacatc gatcgactcg tagttgaggg cgaggtatcg agagagggac ctgatggtga
     2341 taccccggct ggcgagctga gtctttggag tgatgcgcac tctcgtcggc tctatgtggg
     2401 ccagtgagcc acgggccgaa aggagatccc atgctgctct ctcgaagctt gctgcgggcg
     2461 ccggttggga cgaagctaac cctcgacagg cctcgtctgc gatggcgtgc agggtcagga
     2521 gggcacgcag gagtgggcag tcctcgcccc gtcgcagtac agacaacggc gtgtcgcgac
     2581 gttccagcac cactcgccag agcttgacga cgtcggacgg gggactccgc tgcggattag
     2641 cagcggtcac tctccactcg tcagcggctc gttggacccg ttcgttccag tcgcccctcg
     2701 gcggccaacg tgctccgcgt ggcggagcaa ccgtgaaacg atacgcctcg gtgtgatcga
     2761 ggatcagatt ggtgagggcg aagacgtcgg gtggccatcg tgccagctca cgccaagacg
     2821 gcctcgagga gaaggcctcg aagtaggaga ggactgtcgg ttccgattgg gccgtgaacg
     2881 cgacagtatc ggcgttcgga gacgcatgac cggtcgtagc cataccgccc tgctcctatt
     2941 agacatctcc gactatgcga cgaggcggat cggcccaata gggtcgttgt accctttcgg
     3001 cgggatcgaa gattctgccg cgattgtctc tccctgctcg aggacgatgg ccgctgagat
     3061 cgtcccagaa ttcccccgcg gctcatgcct agccacgaac tctcgagatg aacgccgagt
     3121 ctctcggggt gtttgatgcg acctcgccgc ctaatacgac aagctcgttt cgatgtagga
     3181 cgccgtatac caaatgacgc tatagacatc gccgggagtc attggttgag ctcgacttct
     3241 tcggtgcagg cgagtcgaac ggtcggctcg tatgcgacgc caccttgact gacgatcctg
     3301 gccgagatcg atcccactga tccatatgag ttgagtacac ctcgacgtcc tctgcctcga
     3361 ctccattgcg ctgcaggcag tcggcaaaga tc
//
LOCUS       DASUTA010000011         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_131604, whole genome shotgun sequence.
ACCESSION   DASUTA010000011 DASUTA010000000
VERSION     DASUTA010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131604"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VFZ80_00210"
     CDS             complement(<1..511)
                     /locus_tag="VFZ80_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016691868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HEX6285880.1"
                     /translation="MARLGDGGELLKCSFCGKSQKQVKKLIAGPTVYICDECIELCNE
                     IIEEEFSSSEEINFTELPKPAEIKEYLDDYVVGQEEAKRKLSVAVYNHYKRIRSGDIQ
                     RDIELQKSNIVLIGPTGSGKTLLAQTLARQLNVPFAIADATSLTEAGYVGEDVENILL
                     KLLQAADYDV"
     gene            complement(530..1135)
                     /locus_tag="VFZ80_00215"
     CDS             complement(530..1135)
                     /locus_tag="VFZ80_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX6285881.1"
                     /translation="MIPTVFEQTSRGERYFDIYSRLLKDRIIFLGTPIDDNIANLIMA
                     QMLHLESDDPDKDIFLYINSPGGSITSLFAIYDTMQYIKPAVNTVCMGMAASAAAVIL
                     AGGAEGKRFALPHARVMLHQPHGGAQGQATDIEIQARLIVQMREQLNEILAHHSGQDL
                     EKIERDTERDYWMMAAEAKEYGVIDGVLTRRELAAVASSTD"
     gene            complement(1147..>2272)
                     /gene="tig"
                     /locus_tag="VFZ80_00220"
     CDS             complement(1147..>2272)
                     /gene="tig"
                     /locus_tag="VFZ80_00220"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX6285882.1"
                     /translation="IQPAINPELESIDDVDGGVEVEVKVTLWPTIDLPTYKERKIEVT
                     NPAVTEEDIAEQTRRMLEQFGTVEEVERPAGEGDFVSLDLEASSDGERVEDATAEEIL
                     YEVGSGLLVEGIDEQLTGAAAGDVLEFRGPLPAGFGDRAGETVDFKVTVHEVKERILP
                     ELTDEWVDENTEFETVADLREALDEQLADAKLRSASREYSEKALSTLRDQVDIDIPEG
                     LLRAEMDRHLHDFLHRLEESELNLQDYFEASGISQDAFLNDLRNQADLSIRNRLVLDA
                     IIEAEEIEVTEEDMASVLTSLASRSEDPKAYLRAFKESGQELALASDILRNRALDAIL
                     SNADPVDEDGNPVDLSLKVNEVEAEIIDDEEVVAAEEEEE"
BASE COUNT          397 a          681 c          666 g          528 t
ORIGIN      
        1 tgacgtcgta gtcggcagcc tggagaagct tgagcaggat gttctcgacg tcctcgccga
       61 catagccggc ctcggtgaga ctggtggcat cggcaatggc gaaaggaacg ttgagctgtc
      121 gtgccagggt ttgagcgaga agcgtcttac cggagccggt cggtccgatc aggacgatgt
      181 tcgacttctg cagttcgata tcgcgctgga tatcgccaga gcggatgcgc ttgtagtggt
      241 tgtagacggc gacggagagc ttgcgtttcg cctcttcctg gccgaccacg taatcgtcga
      301 ggtattcctt gatctccgcc ggctttggga gctcggtgaa attgatctcc tcggaggagg
      361 agaactcctc ttcgatgatc tcattgcaga gctcgatgca ctcgtcgcag atgtagacgg
      421 tcggaccggc gatgagcttc ttcacctgct tctgcgactt gccgcagaag ctgcatttca
      481 aaagctcgcc accgtcaccg agtcttgcca tgtgagagag ggcctcctgt cagtctgttg
      541 acgaggctac cgcggcgagc tcccgacgtg ttagcactcc gtcgattact ccatattcct
      601 tcgcctcggc ggccatcatc caatagtcac gctcggtgtc acgctcgatc ttctcgaggt
      661 cctgacccga gtggtgagcg aggatctcgt tcaactgctc tctcatttga actatgagac
      721 gggcctggat ctcgatgtcg gtcgcctggc cctgcgcgcc accgtgaggc tggtgcagca
      781 tgacacgggc gtgggggagg gcaaatcgct tgccctccgc tcccccggcg aggatcacag
      841 ctgcagcgga agccgccatg cccatgcaga ccgtgttcac agcgggcttt atgtactgca
      901 tggtgtcgta gatcgcgaaa agcgacgtta tcgagccacc cggcgaattg atgtacaaga
      961 agatgtcctt gtccgggtcg tccgactcca ggtggagcat ctgggccatg atcaggttgg
     1021 cgatgttgtc gtcgatcggt gttcccagga agatgatgcg gtctttgagt agccggctgt
     1081 agatgtcgaa gtaccgctct cctctgctgg tctgctcgaa gacagtcggt atcacgtagc
     1141 tgttgttcat tcctcttcct cctcggccgc aacgacctct tcgtcgtcga tgatctcggc
     1201 ctcgacttcg ttgaccttca aactgagatc gaccgggttt ccatcctcat caaccgggtc
     1261 ggcattcgaa aggattgcgt ccagcgctct gtttctgaga atatcactgg ccagtgccaa
     1321 ttcctggccg ctctccttga aggctcggag atatgccttg gggtcctctg agcgagaggc
     1381 caggctggtc aatacgcttg ccatgtcctc ctcagtgacc tcgatctcct cggcctcgat
     1441 gatggcgtcg agcaccaacc ggtttcgaat agagaggtcc gcttgattac gcaggtcgtt
     1501 caggaaggca tcctggctga tgcccgacgc ctcgaagtag tcctggaggt tgagctcgga
     1561 ctcttcgaga cgatggagga agtcgtggag atgccggtcc atctcggcgc gaagcagacc
     1621 ctcaggtatg tcgatgtcga cttgatcgcg cagcgtgctc aacgcctttt cgctgtactc
     1681 ccgggacgcc gaacggagtt tggcgtctgc tagttgctcg tcgagcgcct ctctcaagtc
     1741 ggcaacggtc tcgaactcgg tgttctcatc gacccattcg tcggtcaact ccggcaggat
     1801 gcgctccttc acttcatgaa cggtgacctt gaaatccacg gtctcaccgg cgcggtcccc
     1861 gaatcctgca ggcagcggtc cgcggaactc gaggacatcg ccagcagccg caccggtcag
     1921 ttgctcatcg atgccttcga cgagaagtcc ggatccgacc tcgtagagga tctcctcggc
     1981 ggtggcgtcc tcgacccgct cgccatcgct cgaagcctcc agatccaggg agacgaagtc
     2041 gccttccccg gcggggcgct ccacttcttc gacggtcccg aactgttcca gcatccgcct
     2101 ggtctgctcc gcgatgtctt cttcggtgac cgccgggttg gtcacttcga tcttccgttc
     2161 cttgtaagtc ggcaggtcga tggtgggcca aagcgtgacc ttcacctcga cctccactcc
     2221 cccatccaca tcgtcgatgg actccagctc cgggttgatg gcaggttgga tc
//
LOCUS       DASUTA010000012         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1816432, whole genome shotgun sequence.
ACCESSION   DASUTA010000012 DASUTA010000000
VERSION     DASUTA010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816432"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..899
                     /gene="era"
                     /locus_tag="VFZ80_00225"
     CDS             18..899
                     /gene="era"
                     /locus_tag="VFZ80_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX6285883.1"
                     /translation="MKSGFVAVVGRPNVGKSTLVNHMVGSKVTITSSRPQTTRNAIRG
                     VVNGDGYQIVLVDTPGLHKPKTELGNRLNSLVYGTLAEADSVIFVVDATMPVGPGDRL
                     IAERLIESGADVVVAVNKVDAASREQTVGQLLEAAEWPFEHYFPLSARTGEGVAEMVS
                     ELVGRLPEGPQYYPDDMLTDQPESMVIAEIIREKFLDRLRDELPHALAVRVEDIEQRE
                     DGLIDISADLIVERNSQKGIVIGKGGSLLKTAGTEARVELEALLGDRVNLNLHVSVEK
                     DWQRTPQLLDRLGFEEL"
     gene            910..1698
                     /locus_tag="VFZ80_00230"
     CDS             910..1698
                     /locus_tag="VFZ80_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6285884.1"
                     /translation="MDLSRYEYLQLDREPDGVLVVTLGDPERLNAVTERGHTELVEVW
                     PEIDRDDDVRAVLLRSKGRAFSAGGSFDMIDHIVEDHATHRRVLKEARELVRNIIECS
                     KPIVSAINGPAVGAGLAAALLADIPVAGRAARIIDGHTNIGVAAGDHAVVIWPLLIGM
                     AKAKYYLMTNEPMDGEEAERLGLVAKVVDDDELDPYTRELTARLANRSSEALAWTKHT
                     LNHWLRTAYPMFDASLAYEFLGFGGPDAREGLAAVKEKRAPTFD"
     gene            complement(1708..1986)
                     /locus_tag="VFZ80_00235"
     CDS             complement(1708..1986)
                     /locus_tag="VFZ80_00235"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX6285885.1"
                     /translation="MSLVPESEVEAFVESHGDWSRADAQISRTYEFADFNEAMGFVTR
                     VALEAEKADHHPDIDIRWNQVTLTLSTHSEGGLTAKDLELAGAVDALV"
     gene            2028..>2198
                     /locus_tag="VFZ80_00240"
     CDS             2028..>2198
                     /locus_tag="VFZ80_00240"
                     /inference="COORDINATES: protein motif:HMM:NF023393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HEX6285886.1"
                     /translation="MAIRHDQGIVLRGYPFGEGDRVVVLLSPNNGKIRTVAKGVRKTK
                     SRFGGRLEPLSHV"
BASE COUNT          409 a          699 c          720 g          370 t
ORIGIN      
        1 gagcaggcag agtcggcgtg aagtcgggtt tcgtcgcggt ggtgggccgc cccaacgtcg
       61 gcaagtcgac cctggtcaac cacatggtcg gctcgaaggt caccatcacc tcctcacggc
      121 cccagacgac ccgcaacgcg atcaggggcg tggtcaacgg tgacggctac cagatcgtcc
      181 tggtggacac gcccgggctg cacaagccca agaccgagct cggcaacagg ctgaactccc
      241 tggtctacgg cacactggcc gaagccgact cggtgatctt cgtggtcgac gccacgatgc
      301 ccgtggggcc aggcgaccgg ctgatagcgg agcgtctcat cgagagtgga gccgatgtcg
      361 tcgtggcggt caacaaggtc gacgccgcca gccgggagca gacggtagga cagctattgg
      421 aggcggctga gtggcctttc gagcactatt tccctctttc ggcacgcacc ggggagggtg
      481 tggccgagat ggtgtccgag ctggtcgggc gtcttccgga gggaccgcag tactaccccg
      541 acgacatgct caccgaccag cccgagtcga tggtcatcgc cgagatcatc agggagaagt
      601 tcctcgatcg tctccgtgac gagctgccac acgccctcgc ggtccgtgtc gaggacatcg
      661 agcaacgcga ggacggcttg atcgacatct ctgccgatct gatcgtcgaa cgcaacagtc
      721 agaagggcat cgtcatcggg aaaggcggga gtctgctcaa gaccgcagga accgaggcac
      781 gggtagagct ggaggccctg ctcggcgacc gggtcaacct caacttacat gtgtcggtgg
      841 agaaggactg gcagcggact cctcagctgc tcgaccgtct cggcttcgaa gagctctagg
      901 ttggccgcaa tggacctcag tcgatacgaa tacctgcaac tcgaccggga gcccgacggg
      961 gtgctcgtgg tgacactcgg cgatccggag cgactcaacg cggtgaccga gcgcgggcac
     1021 accgagctgg tggaggtctg gcccgagatc gatcgggacg acgatgtcag agccgtcctc
     1081 ctccgctcca aaggccgtgc gttctcggct ggaggatcgt tcgacatgat cgaccacatc
     1141 gtcgaggacc acgccaccca ccgtcgtgtc ctcaaagagg cacgggaact ggtcaggaac
     1201 atcatcgagt gctccaagcc gatcgtgtcc gcgataaacg ggccggccgt gggcgccggt
     1261 ctggccgccg ctctcctcgc cgacatcccc gtcgccggtc gggcggcgcg catcatcgac
     1321 ggtcacacca acatcggtgt cgccgccggt gaccatgccg tggtgatctg gccactcctc
     1381 atcgggatgg cgaaggccaa gtactacctg atgaccaacg aaccgatgga cggcgaggag
     1441 gccgaacggc tcgggctggt agccaaggtg gtggatgacg acgagttgga cccctacacg
     1501 agagaactga ccgctcgact cgcgaaccgc tcctcggagg cgctggcgtg gaccaagcac
     1561 accctcaacc actggctccg gacggcgtac ccgatgttcg atgcctcgct ggcatatgaa
     1621 tttctcggat tcggtggtcc cgacgcccgc gagggtcttg ccgcggtcaa ggagaagcgg
     1681 gcccccacgt tcgactgaac cggcgcttca gacgagcgcg tcgaccgctc cggcgagctc
     1741 gaggtccttg gcagtaagac cgccttcgga gtgggtcgag agggtgaggg tcacctggtt
     1801 ccagcggatg tcgatgtcgg gatggtgatc ggccttctcc gcctcgagag ccaccctggt
     1861 cacgaatccc atcgcctcgt tgaagtcggc gaactcgtag gttcggctga tctgggcgtc
     1921 ggctctggac cagtccccat ggctctccac gaaggcttcg acttcgctct ctggcacgag
     1981 tgacatgcac cgagactagg cggtgacccc gataccctgt cgccctcatg gcgattcgac
     2041 acgaccaggg gatcgttctc aggggctatc ccttcgggga gggggaccgt gtcgtcgttc
     2101 tcctcagccc gaacaacggc aagatccgca ccgtcgccaa aggcgtccgg aagaccaaga
     2161 gccggttcgg cgggcgtctc gagccgctct cgcacgtc
//
LOCUS       DASUTA010000013         3722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1817107, whole genome shotgun sequence.
ACCESSION   DASUTA010000013 DASUTA010000000
VERSION     DASUTA010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1817107"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VFZ80_00245"
     CDS             complement(<1..507)
                     /locus_tag="VFZ80_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6285887.1"
                     /translation="MGPPSLIAFDVNETLLDLAPIKTNLEGVFGVDPPMGEWFARLLH
                     GSLVANTLGQFRSFGDIAAEALINIAVRRGLLLRAEDALEALAPMSRLSPHPDVVPGV
                     RRLAEAGKRMVALTNGSREVANSQIHNAGLAEYLERVISVDEVGVFKPDPAPYRHTAE
                     LMGVPIEEM"
     gene            complement(530..1768)
                     /locus_tag="VFZ80_00250"
     CDS             complement(530..1768)
                     /locus_tag="VFZ80_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HEX6285888.1"
                     /translation="MSSPTVLVVGGGPAGSAAAYWMARDGLDVTLVEKKEFPRDKTCG
                     DGLTPRAILQLQDMGFDFDVAEFHRITGLRSYAGDDLMIEMPWPEHSRFPNWGGVIRR
                     RDLDQQVARLAEKEGVRVREKTTATPIIEEGRLTSVALSNGGAEEVITPDVTIIADGS
                     MNRFGRELGTERRRDYPLGMAARGYYASPRSEDPFLESQLDLRDANGNTMPGYGWVFP
                     LGDGTVNVGVGLLSTFRHWKEVNTTNMMNDYVATAPQYWGLSEETRLSKPMGGKLTMS
                     YSKGPLVGHNWLTIGDAAGAINPWNGEGISYAYETGRIAAEYVSEALGADDPGLLRRY
                     PQRLADEYGLYYKMARIFVKLIGYPSVMRTLAHTGLRNRPLMEWTLKVMANLLDEDDK
                     HMGERTYDALASVVRTLPVK"
     gene            1793..2440
                     /locus_tag="VFZ80_00255"
     CDS             1793..2440
                     /locus_tag="VFZ80_00255"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX6285889.1"
                     /translation="MGNQEARRYRLSSMSVLSRATVSRLPRYLRLLDDLRESQTTVSS
                     GELADAAGVNPANVRRDLSDLGFQGTRGVGYSVGDLRTRIQRELGISGRRRVAIVGAG
                     NLGTALARYSGLSQRGFDVVAIYDVDQSRIGQPVGDLTVSKAASIGEDCRRGLFDMAI
                     LAVPAGAAQQVADQLVSSGVSSILNFAPARVTVPEEVPVRQVDLSTELQILSYYG"
     gene            complement(2470..2832)
                     /locus_tag="VFZ80_00260"
     CDS             complement(2470..2832)
                     /locus_tag="VFZ80_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020020192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX6285890.1"
                     /translation="MFFRELFRSLLSANVFVTGIILTLASVLLFYGSIYLFNYTNLGK
                     RLAFLVTGAAVFGWLSITSMLFVIYAPRGPKPDNIEGLNAFEIRIIPITFLLVSVVLF
                     LVFLVALHQYEETKEAGG"
     gene            complement(2835..>3722)
                     /locus_tag="VFZ80_00265"
     CDS             complement(2835..>3722)
                     /locus_tag="VFZ80_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6285891.1"
                     /translation="FEHFQVFQPIALDPAVADAELVDEGLAELESLSEADPIFQVSIA
                     GVEAALEADEITEDSPDTDGDGISDAAEATISAIFTEASAATVPDEITVVELDPTNPQ
                     TVPDVADLQTATTMVGGLETVNINLGVTVDNFSRIYEQQLEGLQYLQDAQQAAAWEID
                     IAGVAEAMSASEEEAARAVALFNGNCARCHTAGFSAGVPYTLEAGSGGFGPALWDGRP
                     IVQFGDMPAGEEQPDLLIDFITNGSEAQTPYGLNGFGSGRMPGFGEILDAADIQLLAR
                     YLRGGNLNGMNPPEPEPGS"
BASE COUNT          672 a         1174 c         1203 g          673 t
ORIGIN      
        1 catctcttcg atgggcaccc ccatcaactc ggcggtgtgt cgataagggg cggggtcggg
       61 cttgaagaca ccgacctcgt cgaccgagat gactcgctcg aggtattcgg cgagaccggc
      121 attgtgaatc tgggagttgg cgacttcccg gcttccgttg gtcaaagcca ccatccgctt
      181 cccggcctca gctagtcgcc gcaccccggg gaccacgtcg ggatgcggcg agagacggct
      241 catcggggcc agggcctcga gggcatcctc ggcacggagc aggagcccac gccggacagc
      301 gatgttgatc aaggcctcgg cggcgatgtc gccgaaggat cgaaactggc cgagtgtgtt
      361 ggcgacgagg gatccgtgga ggagccgggc gaaccattcc cccatgggag ggtcgacccc
      421 gaacacccct tccagattcg tcttgatcgg cgcgagatcg agcagcgtct cgtttacgtc
      481 gaatgcaatg aggctcggag ggcccatgcc ccctcatggt agatgggccc tacttgacgg
      541 gaagggtgcg aacgacgctg gcgagggcgt catatgtccg ctcgcccatg tgcttgtcgt
      601 cctcgtcgag caggttcgcc atcaccttga gggtccactc catgaggggc cggttgcgga
      661 ggccggtgtg cgccaaggtg cgcatcaccg acggatagcc gatgagcttc acgaagatcc
      721 gggccatctt gtagtaaagg ccgtactcgt ctgccagacg ctggggatac cgccgcagca
      781 gacccggatc gtcggcccca agcgcttcgc tcacatactc ggcggcgatc cggccagtct
      841 cgtaggcata ggagatgcct tcgccgttcc atgggttgat cgccccggca gcgtctccga
      901 tcgtcagcca attgtgcccc accagcgggc ccttggagta cgacatcgtc agtttgccgc
      961 ccatgggctt cgacagcctc gtctcctcgc tcaaccccca gtattgcggt gccgtcgcca
     1021 cgtagtcatt catcatgttg gtggtgttga cctctttcca atgccggaaa gtggacagca
     1081 gcccgacacc cacattgacc gtcccatcgc cgaggggaaa cacccatccg tacccaggca
     1141 tggtgtttcc gttcgcgtca cgaagatcga gctggctctc cagaaacggg tcctccgagc
     1201 gcggcgaggc gtagtagccg cgagcggcca tgcccagcgg gtagtcacga cgacgctccg
     1261 ttcccagctc acgcccaaaa cggttcatcg atccgtcggc gatgatcgtc acatcggggg
     1321 tgatgacctc ctcggcgccc ccgttggaca gtgcgaccga ggtcaggcgg ccttcttcga
     1381 tgatcggtgt cgccgttgtc ttctcacgaa cccggacccc ttccttctcg gcgagacggg
     1441 cgacctgttg gtcgagatcc ctgcgtcgga tgacaccgcc ccaattgggg aagcgactgt
     1501 gctctggcca gggcatctcg atcatgaggt cgtcgcctgc gtaggagcgc aaaccggtta
     1561 tacggtggaa ctcggcaacg tcgaagtcga accccatgtc ctggagctgc aggatcgccc
     1621 tcggagtcag gccgtcgccg caggtcttgt cccgcgggaa ctccttcttt tcgaccaggg
     1681 tcacgtcgag gccgtcgcgg gccatccaat acgcggccgc cgaaccggca ggaccgcctc
     1741 cgaccacgag aacggtgggt gaggacatgc ccaagagatc gtaaatggtc gaatggggaa
     1801 ccaggaagcc cgccggtatc gtctctcctc gatgagcgtg ctttcccgcg ccactgtctc
     1861 ccgattgccg aggtatctcc gactgctcga cgatctacgt gagtcgcaga cgacggtcag
     1921 ctccggcgag ctcgccgatg cggccggggt gaatccggcc aacgttcgtc gagacctgtc
     1981 cgatttgggg ttccagggca ccaggggcgt gggctactcg gtgggagacc tgcgcacccg
     2041 catccagcgc gagctgggga tcagcggacg acgcagggtc gccatcgtgg gagccggcaa
     2101 cctgggaact gctctcgccc gctacagcgg tctcagccag agaggttttg acgtcgtcgc
     2161 catctacgac gtcgaccaga gtcggatcgg ccagccggtt ggcgacctga cagtctcgaa
     2221 ggccgcatcg atcggggagg actgccgccg agggctgttc gacatggcga tcctcgccgt
     2281 gcctgccggg gccgcgcaac aggtcgccga ccaactggtg tcctccggag tctcctccat
     2341 cctcaatttc gccccggcgc gggttacggt ccccgaggaa gtccccgtcc gtcaggtcga
     2401 cctctcgacc gagctccaga tcctcagcta ctacggctag gggaggcttt agaacctcga
     2461 acccgggggc tagccgccgg cttccttggt ctcctcgtac tggtggaggg cgaccaggaa
     2521 gacgaggaag aggaccacgg acaccaacag gaaggtgatc gggatgatgc ggatctcgaa
     2581 cgcgttcaga ccctcgatgt tgtccggttt cggtccccgg ggggcataga tcacgaagag
     2641 catcgacgtg atggagagcc atccgaacac ggcggcaccg gtgaccagga aggcgagacg
     2701 cttcccgagg ttggtgtagt tgaagagata gatcgacccg tagaagagga ggacggaagc
     2761 gagggtgagg attatcccgg tgacgaagac gttcgccgag aggagtgatc tgaacagttc
     2821 tctgaagaac atggctaact ccccggctcc ggctccggcg ggttcatccc gttgagattg
     2881 cccccccgaa ggtagcgggc cagcagttgg atatcggcag cgtcgagtat ctcaccgaag
     2941 ccaggcatcc gacccgaccc gaacccgttc aggccgtagg gcgtctgcgc ctctgagccg
     3001 ttggtgatga aatcgatcag gagatccggt tgttcctcgc ctgccggcat gtcgccaaac
     3061 tggactatgg gccggccatc ccacagtgcg gggccgaagc caccggaacc ggcctcgagg
     3121 gtgtaaggga caccggcact gaaaccagcg gtgtggcaac gagcacagtt gccgttgaag
     3181 agggccactg cacgggccgc ctcttcctcc gaggcggaca tcgcctcggc gacgccggcg
     3241 atgtcgatct cccacgccgc ggcctgctgt gcgtcctgga ggtactgcag accctcgagc
     3301 tgttgttcgt atatgcgact gaagttgtcc accgtcaccc cgaggttgat gttcacagtc
     3361 tccaaccctc cgaccatcgt cgtggccgtc tgcaggtcgg ccacatcggg gacagtctgc
     3421 gggttggtcg ggtccagctc gacgacggtg atctcatcgg gaacggtggc ggcgctcgct
     3481 tcggtgaaaa tcgccgagat ggtggcctca gccgcatcgg agatgccgtc accgtcggtg
     3541 tccgggctgt cctcggtgat ctcgtcggcc tccagggcgg cctccacacc cgctatcgag
     3601 acctggaaga tggggtcggc ctcggaaaga ctctcgagct cggcgagacc ctcgtccacg
     3661 agttcggcat cagcgactgc agggtcgagc gcaatgggct ggaacacctg gaagtgctcg
     3721 aa
//
LOCUS       DASUTA010000014         2645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1169687, whole genome shotgun sequence.
ACCESSION   DASUTA010000014 DASUTA010000000
VERSION     DASUTA010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169687"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFZ80_00270"
     CDS             <1..528
                     /locus_tag="VFZ80_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6285892.1"
                     /translation="AFVVGHSYGAVCSLEAALLTDRIHKLALYEPPIPTGVQMYPPGV
                     PDRMQSLVDAGDWERALELFFQEVVRMPPHELSQYRQLPMWRRRVQLAPTIPREMTLD
                     RTYRFEPDKFAGLSIPTLLLLGGDSPPMFEKAVEAVHDAVPSSTVVILPGQQHIAMDT
                     NPELFLRELTRYLDE"
     gene            complement(556..843)
                     /locus_tag="VFZ80_00275"
     CDS             complement(556..843)
                     /locus_tag="VFZ80_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285893.1"
                     /translation="MTVDDSPPNPGTGANISGWAGVVLYLVLGFVYLTSGLVVPLPGL
                     VVLWLVWAAGWFLVVRLFRTRRIWTPVVALAGTVFWWVYLSVGEALFDWTA"
     gene            complement(1017..1859)
                     /locus_tag="VFZ80_00280"
     CDS             complement(1017..1859)
                     /locus_tag="VFZ80_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285894.1"
                     /translation="MQFKGHLLLPDETGPGLRVDLDVAAHHLAVESDGGGLGAWPLEV
                     VGVKRNGDVFDLTVAGELLRFVADDTIAFAYTGVPTIESVSRRPRKRPAFRSWVDRLW
                     NGPGDSRPAEGPEPTQAAGADRPFEEVEGPPSNFEGAPLAEPVVGLIDEGDLADLDPE
                     DRRFDDPLVVEESGIPTPDTAICPAIRSDGLRCESSILTESGYCYPHDPKRAFSDKYR
                     DAQAARKQLKRDATARLNRVYGRLDKAMRQVERGELDPEAAMAMAQLARTMCAILEVD
                     ESGT"
     gene            complement(2156..2362)
                     /locus_tag="VFZ80_00285"
     CDS             complement(2156..2362)
                     /locus_tag="VFZ80_00285"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HEX6285895.1"
                     /translation="MSRQHEEPKAEGWYDDPEGRKGIQRYWNGSSWSGAPRHKPPQLT
                     GSAIAAMIVGTVVFAALMWWLVFS"
BASE COUNT          520 a          863 c          798 g          464 t
ORIGIN      
        1 gccttcgtcg tcgggcactc ttacggcgcc gtgtgcagcc tcgaagcggc gttgctcacg
       61 gaccggatac acaagctggc cctgtatgag ccgccgatcc ccacaggtgt gcagatgtac
      121 ccgccagggg tgcccgatcg aatgcagtcg ctggtagatg caggcgattg ggagagggct
      181 ctggagctct tcttccaaga ggttgtgcga atgcctccgc acgagctctc tcagtatcgc
      241 cagttgccca tgtggaggcg aagggtgcag ctggcgccga ctattcccag agagatgacc
      301 ctcgatagga cgtatcgctt cgaaccggac aagtttgccg gtctgagcat ccccaccttg
      361 cttctcctcg gcggcgacag tccgcccatg ttcgaaaagg ccgtcgaagc ggtccacgac
      421 gctgtgccca gttccaccgt ggtgatactc cccggtcaac agcacatcgc gatggacacc
      481 aaccccgagt tgttcttgag agaactgact cgctatctcg acgagtgaac gcagattggg
      541 gaaggagcct ggctttcagg cggtccagtc gaacagagcc tcgccgacgc tcaggtagac
      601 ccaccagaac actgttccgg cgagggcaac gaccggagtc cagatccggc gggtgcggaa
      661 gagacgcacc acgaggaacc atccggcggc ccacaccagc cacaaaacca ccaaccccgg
      721 cagcgggacc acgagaccac tggtcaggta cacgaaaccc aacaccagat acaggacgac
      781 ccccgcccat ccgctgatat ttgccccggt gcccggattg ggcggagagt catcaacagt
      841 catcgtcgga ttatgaccga cctcgattac gacttgatgg cttcgctgca gatcagcggt
      901 cacaccccat tcagtgcacg gggaagacca ccgggttggc acaagccgcg tgcgttcatg
      961 gttggaggac gcaggggttc gacagtggcg ggtccggact ggcctttcag cgtttttcag
     1021 gtcccggact cgtcgacctc gaggatggcg cacatcgtcc tggccagctg tgccatcgcc
     1081 atcgccgcct ccgggtccag ctcgcctctc tccacctgcc gcattgcctt gtccagtcgt
     1141 ccgtagaccc ggttgagacg cgccgtggca tcccgcttca actgctttct ggccgcttgt
     1201 gcgtctctgt acttgtcgga gaaggcgcgc ttggggtcgt ggggatagca atagccagac
     1261 tcggtgagga tcgaagactc acaacggaga ccgtcactcc ggatggccgg acagatggcg
     1321 gtgtccggag tcggaatacc tgactcctcc accactagtg gatcatcaaa acgtcgatcc
     1381 tccgggtcca aatcggccag atccccctcg tcgatcagac cgacgaccgg ctcagccagg
     1441 ggcgcccctt cgaagttcga aggaggaccc tcgacctctt caaatggccg atcggcgccg
     1501 gcggcttggg tgggctctgg accctcggcc ggtctcgagt cccccgggcc gttccacagt
     1561 cgatccaccc aagaacgaaa cgccggacgc ttccgtggtc gccgggagac gctctcgatg
     1621 gtgggtactc cggtgtaggc gaaagcgatc gtgtcgtcgg cgacaaaccg gagtagctct
     1681 ccagccacgg ttaggtcgaa cacatcgccg ttgcgcttca ccccgaccac ctccaacggc
     1741 caggctccga ggccaccacc gtccgactcc actgcgagat gatgagccgc cacgtcgaga
     1801 tcgaccctca agccggggcc ggtctcgtca ggcagcaaca gatggccttt gaactgcaag
     1861 tccgaatcct cctcggatca gtgtcggcag aatgggtgtg actcttgaac cgtcacctgg
     1921 gcgaaacgac ccaatttccg gctttcgtcg aagaaccacg ccgacgttgc aatcgagccc
     1981 gaatgaccga taccggcatc tccgcaaccc gttgccgcct tcacatcatt gcctggtgtg
     2041 ggggaggtca gccacggcca tttcgaggag ctgaatccca tggcccatgg tccgtcgagg
     2101 gccgagggtg agcaccccgt tcggtcctgc ggaggcaccg ctcggccacg tggcatcagc
     2161 tgaagacgag ccaccacatg agggctgcga agacgacggt cccaacgatc atggcagcga
     2221 tagccgagcc ggtcagctgt ggcggcttgt gtcggggagc accgctccac gagctcccgt
     2281 tccaatagcg ttgaattccc ttgcggccct cgggatcgtc gtaccaaccc tccgccttcg
     2341 gctcttcgtg ttgcctggac atggtcgcgt gaagctagtt cctggcctga cgtgacacca
     2401 tctgatctcg tcctcatacg aatcggagcc cagtcggcgg cgtgagccga tccagtgtgg
     2461 ggacaaggcg gcccagacca gcaaacctag ggcgacggcg cagagataga tgagaccgac
     2521 gggcagccat gggatggcgg ggtcggttct tgccgaaacg gctaccgcca cgcccaaccc
     2581 gaggccgaag actgctccaa cggcagccga caccggcatt cgcacaagcg acgccagggc
     2641 gacga
//
LOCUS       DASUTA010000015         2579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1429197, whole genome shotgun sequence.
ACCESSION   DASUTA010000015 DASUTA010000000
VERSION     DASUTA010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429197"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1717)
                     /locus_tag="VFZ80_00290"
     CDS             complement(<1..1717)
                     /locus_tag="VFZ80_00290"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX6285896.1"
                     /translation="MANRLASATSPYLLQHKDNPVDWWEWSDDAFTEARRRDVPVLLS
                     VGYAACHWCHVMAHESFEDDATAKVMNERFVNIKVDREERPDVDSVYMEAVQAMTGQG
                     GWPMTVWIDHEGRPFFAGTYYPRQPRGGMPSFSQVMDAVWAAWTERRDDVSEQAGRLV
                     AAISREVPGGAIPAEAELADAYDKIESSFDPVHGGFGHAPKFPQQPVLEYLLRIHGEE
                     WAPNAATMLAKTLDEMAAGGIHDQIGGGFARYSVDAHWLVPHFEKMLYDNAQLIHLYL
                     WAGLEMEKPALVEVARRTIEYIERDLRHPSGGFFSSEDADSEGVEGKFYVWTPEEIRA
                     VLGDSSEDVIQYYGVTEKGNFEGSNILNVVRDAEPAGLDRARSALLEARSRRVRPGLD
                     DKVVTSWNGLVIRALSEAGAALGEGHYLDLARQAASFVLGELVVDGALKRSWREGRAS
                     VPGFLEDHAAMAVGLFTLYSATGEERWYEEAMSLVTALEQFARPEGGFFATSGQAENL
                     IKRPTDITDNPLPSGNALAAEALLLAHLYTGDQAWRDSAVAALESIALLADRYPSMVG
                     HHLAVS"
     gene            1787..>2579
                     /locus_tag="VFZ80_00295"
     CDS             1787..>2579
                     /locus_tag="VFZ80_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX6285897.1"
                     /translation="MTTETLQEVVNSPFDPWDESHRSDPKPFYKAMRESAPVYAGIGP
                     VTRRRFWFLTRYDDVVDALRNPELGREWKKLPEEVREQHHFEEQDEFELVNRHLLNLD
                     PPDHTRLRRLVSHAFTSRRIAELEPRIRDLTDRLLDDLADGDDLVEKLALPVPITVIA
                     ELLGVPIDDQAQFRDMVDRSLRGSTDEEMMAAGLELLAYVNEAIEYRRAKPGDDLLSA
                     LIHLEEDGDTLDHSELLSMVQLLLIAGHETTVNLIGNGMIELMRHP"
BASE COUNT          461 a          858 c          811 g          449 t
ORIGIN      
        1 gggagacggc gagatggtgg ccgaccatcg aggggtagcg atcggcgaga agcgcgatcg
       61 attccagggc agcgacggcc gagtcccgcc acgcctggtc tccggtgtac aggtgagcca
      121 ggagcaacgc ctccgcggcc agggcgttgc ccgacggaag gggattgtcg gtgatgtccg
      181 tcggccgctt gatcaggttc tccgcctgcc ctgaagtggc gaaaaagcct ccttccgggc
      241 gggcgaactg ctcgagagct gtcaccaggc tcattgcttc ctcgtaccac cgctcctctc
      301 ccgtggctga gtacagggtg aagagaccga cggccatggc ggcgtggtct tcgaggaaac
      361 cgggcaccga tgcccgcccc tcccgccagg accgcttgag ggccccatcg actaccagct
      421 cgcccagcac gaacgaggca gcttggcgcg ccaggtccag gtagtgccct tcgccgagtg
      481 cggcgccggc ctcggaaagg gcacgaatga cgagcccgtt ccaggacgtg accaccttgt
      541 cgtcgagacc cggacgaacc cgtcgagatc tggcttcgag caaggctgag cgagcccgat
      601 ccaggccagc gggctcggca tctctgacca cgttgaggat gttggaccct tcgaagttgc
      661 ccttttccgt gacgccgtag tactggatca cgtcctcgga ggagtctccc agcacggccc
      721 ggatctcctc cggagtccag acgtagaact tgccttcgac cccctcggag tcggcgtcct
      781 ccgacgagaa aaaaccgccc gatgggtgtc gcaagtcacg ttcgatgtac tcgatggtcc
      841 gccgggccac ctcgaccagg gccggcttct ccatctcgag cccggcccag aggtacaggt
      901 ggatcagctg ggcgttgtcg tagagcatct tctcgaaatg gggcacgagc caatgcgcat
      961 ctaccgagta ccgggcgaat ccacccccta tctggtcgtg gatcccgccg gctgccatct
     1021 catccagagt cttggccagc atcgtggccg cgttcggcgc ccactcctcg ccatgaatgc
     1081 gaagcaggta ctccagcacc ggttgttgtg ggaacttcgg tgcgtgcccg aagccaccgt
     1141 ggacggggtc gaagctcgac tcgatcttgt cataggcgtc ggcgagctcg gcctcggcag
     1201 ggatggcacc gcctggcacc tcccgcgaga tggctgcgac gagacgacct gcttgctcgg
     1261 agacgtcatc tcttcgctcg gtccaagccg cccagaccgc atccatgacc tgactgaagc
     1321 tcggcatgcc accgcgcggc tgtcggggat agtaagtgcc ggcgaagaac gggcgacctt
     1381 cgtggtcgat ccagaccgtc atcggccagc ccccctgtcc ggtcatcgcc tggactgctt
     1441 ccatgtatac ggagtcgacg tccggtctct cctcccggtc gaccttgatg ttgacgaatc
     1501 gttcgttcat gaccttcgcc gtggcgtcgt cctcgaacga ctcgtgtgcc atgacgtgac
     1561 accaatgaca ggcggcgtat ccgaccgaga gcagcaccgg gacgtcccgc ctcctcgcct
     1621 ccgtgaaggc gtcgtcggac cattcccacc agtcgacggg gttgtccttg tgctggagga
     1681 ggtagggaga tgtagcggag gcgagccgat tggccatcgc ctgcaacggt aggtggaccg
     1741 cgggagcaga tgatccaggt cgccctacac ggcctagcct tgcctcgtga ccaccgagac
     1801 cctgcaggaa gtcgtcaaca gccctttcga cccctgggat gagagtcacc ggagcgaccc
     1861 caagcccttc tacaaggcga tgagagaatc ggcgccggtc tatgcgggga taggtcccgt
     1921 cacccgtcga cggttctggt tcctcacccg atatgacgac gtggtcgacg ctctgcgcaa
     1981 ccccgagctc ggacgtgagt ggaagaagct cccggaggag gtgcgtgagc agcaccactt
     2041 cgaggagcag gacgagttcg aactggtcaa ccgccacctc ctcaacctcg accctccaga
     2101 ccacaccagg ctgcggcggc tcgtgtccca tgccttcacc tcccggcgca tcgccgagct
     2161 cgagccgagg atccgggacc tgacggaccg cttgctggat gatctggccg acggcgacga
     2221 cctggtcgag aagctggcgt tgccggtccc gatcaccgtc atcgccgagc tcctcggcgt
     2281 ccccatcgac gaccaggcgc agttccggga catggtcgat cgcagtctcc gtggctccac
     2341 cgacgaagag atgatggccg ccgggttgga gctgctcgcc tacgtgaacg aggcgatcga
     2401 gtaccggcgg gcgaaaccgg gagacgatct gctgtccgct ctcatccatc tcgaagagga
     2461 cggcgacacc ctcgaccact ccgagctctt gtcgatggtc cagctgctcc tgatcgcagg
     2521 tcacgagacc accgtcaacc tgatcggcaa cgggatgatc gagctgatgc ggcaccccg
//
LOCUS       DASUTA010000016         2271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1169960, whole genome shotgun sequence.
ACCESSION   DASUTA010000016 DASUTA010000000
VERSION     DASUTA010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169960"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VFZ80_00300"
     CDS             complement(<1..616)
                     /locus_tag="VFZ80_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014139867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6285898.1"
                     /translation="MSDRRITARAFHDSGGVEDWRVLYWGAYAHYRTSSFVEGCRFVT
                     AIAEVAEEVGHFPDVDLRPEGVTIRTFSRLDGALGEADVDLARRITVLAVERELKADP
                     SRLQAVGIAVAQDAEADVRPFWAAAFGYSELGEEDAVDPNRRNPHLWFHDLEPPRSGR
                     GRVHIDVSVPADQAEARVAAALAAGGRIADDSHAPEWWVLASPEN"
     gene            complement(773..997)
                     /locus_tag="VFZ80_00305"
     CDS             complement(773..997)
                     /locus_tag="VFZ80_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285899.1"
                     /translation="MAEIERGVEVSGEHGRDLKVGIQIANGPETYRLMLTRPLDKEGR
                     IVEIPLEAIEEIYEIEPGPRGAPPEIVDED"
     gene            1120..1359
                     /locus_tag="VFZ80_00310"
     CDS             1120..1359
                     /locus_tag="VFZ80_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285900.1"
                     /translation="MSPTRRTFDETDDERRPLQLGDRYEIVHGPASSPERTTDGVLVD
                     IDQTKKTYTFELEDGSRISVADDEIDGVRRLAGDR"
     gene            1619..2029
                     /locus_tag="VFZ80_00315"
     CDS             1619..2029
                     /locus_tag="VFZ80_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX6285901.1"
                     /translation="MEHEYARKLLADKRGGLESLQEQMRESSELDESQQESAGALSSH
                     DQHPADIATQTFQREQDRSIEERLEDEIAEVDHALEKISRGEYGKCEQCGKTIADERL
                     EILPATRYCLEHAPRQTGPAAEAANEVTQELDQV"
     gene            complement(2092..>2271)
                     /locus_tag="VFZ80_00320"
     CDS             complement(2092..>2271)
                     /locus_tag="VFZ80_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020139284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX6285902.1"
                     /translation="VVELSPDFASGTVVDAITDDDFDIPTTVAEFGNSLYAVNARFTT
                     APGPSVEYDVVRVGK"
BASE COUNT          459 a          723 c          693 g          396 t
ORIGIN      
        1 gattctccgg tgaagccagg acccaccact caggagcgtg cgagtcgtcg gcgattcgac
       61 caccggcagc cagggcggcg gcgaccctgg cctcggcctg atcggcgggg acactgacgt
      121 cgatgtggac ccggccacgg ccgctcctgg gtggctcgag gtcgtggaac cacaaatggg
      181 ggttccgccg gttcggatcg accgcgtcct cctcgccaag ctcgctgtag ccaaatgccg
      241 ctgcccagaa gggtcgaaca tcggcctcgg cgtcctgggc cacggcgatc ccgaccgcct
      301 ggagcctcga tgggtccgct ttcagttcac gctcgacggc caatacagtt atgcgacggg
      361 cgaggtcgac atcagcctcg ccaagggcgc catcgagccg gctgaatgtg cggattgtca
      421 ctccttccgg tcgcaggtcg acatccggaa agtggcccac ctcctctgcc acttcggcaa
      481 tcgccgtcac gaatcgacaa ccttcgacga atgaggacgt tcgatagtgc gcgtaggcac
      541 cccagtagag aacccgccag tcttcgacgc cgccggagtc gtggaaggcc cgcgccgtga
      601 ttcttctgtc gctcatggat gcacctttct ggacggcctg acacggctga agtattagcc
      661 ggtggcaggc ccggtgcagc tcgtctcgtt gtcgacagcg aggcccggct cggtccgtac
      721 ggtctgacgc ccaccgagaa gttggggaac aatcgaggag agcgtccgac actcagtctt
      781 cgtcgacgat ctccggaggt gcccccctcg gtcctggctc gatctcatag atctcctcga
      841 tcgcctccaa agggatttcg acgatgcggc cctccttgtc gagcgggcgg gtgagcatga
      901 gccggtaggt ctcagggcca ttggcgattt ggattcccac tttgaggtcg cgcccgtgct
      961 ctccgctcac ctcgacgccg cgctcaatct cggccatggt cgccatttac cgcacctgaa
     1021 gcgtggccaa acctcgatcc ggcaaggagt ggactacacg ctggtttgtc gccctttcca
     1081 atgggttata gctgtcgctc ccaaaggaac tgagtaccca tgagcccgac gcgacgaacc
     1141 ttcgacgaga cagacgatga acggcgacca ttgcagctgg gtgatcgcta cgagatagtg
     1201 cacggtccgg catcctcacc ggagcgaact actgatggcg tactcgtcga catcgaccaa
     1261 acgaagaaga cctacacctt cgaattggaa gatggcagtc ggatctcggt ggcagacgac
     1321 gagatcgacg gggtcagacg gctcgcaggt gatcgctgat cgccatcgtc accgaatgcc
     1381 atcgaccctg caacgggtag ccggccggca aacgaagggt tgacaatccc cgaagccgcg
     1441 tcatggatct gggtcttggg caacgcaccc gttgagacca ttgagctcgc tgtcacatcc
     1501 gagacgacgg gcaccggcgc cgccgtcctc gtacccatgt cgatatcggg gagttcgtcg
     1561 gcgagctcgg ctgaaaggag cctgcatggg attcgtccgt tgcgggtaac agcgaagtat
     1621 ggaacacgaa tacgccagga aactcctcgc cgacaagcga ggcggcctgg agtcccttca
     1681 ggagcagatg cgcgagtcga gcgagctgga cgaatcgcaa caggaatccg ctggagcgtt
     1741 gtcctcacac gaccagcacc cagccgacat cgccacacaa acgtttcaac gagaacagga
     1801 tcgttcgatc gaagaacggc tcgaggatga gattgcggag gtcgatcacg ccctcgaaaa
     1861 gatctcgcgc ggcgagtacg gcaagtgcga gcaatgcggc aagaccatcg ccgacgagcg
     1921 gctcgagatc cttccggcca ccaggtattg cctggagcac gctccacgcc aaaccggtcc
     1981 cgccgccgaa gcggccaacg aggtcaccca agagctagat caggtctgaa cgcatgcagg
     2041 tcgagacgag ccccccgctc gagcactccc cgagggcggg ctctctcgtt gtcacttccc
     2101 gactctcaca acgtcgtatt cgacgctcgg gccgggagcc gttgtgaacc gagcattgac
     2161 cgcatacaac gagttgccga actcggccac cgttgtcggt atgtcgaaat cgtcatcggt
     2221 gatcgcatcg accacggtgc ctgaggcgaa atctggcgac agctcgacga c
//
LOCUS       DASUTA010000017         2599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1690273, whole genome shotgun sequence.
ACCESSION   DASUTA010000017 DASUTA010000000
VERSION     DASUTA010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690273"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VFZ80_00325"
     CDS             complement(<1..428)
                     /locus_tag="VFZ80_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX6285903.1"
                     /translation="MATDQHVNPIAPRPQLITYPDSLGGDLKSLSELMQGPLAGLFRG
                     VHILPPFPSSGDRGFSPIRYDEIDPLFGTWADIEQIAANHDVLLDVMVNHISRQSPQF
                     QDFVQRGRKSAYSDLFITLDKVWPDGDPPHSDVARLFLRKP"
     gene            complement(578..1342)
                     /gene="kduD"
                     /locus_tag="VFZ80_00330"
     CDS             complement(578..1342)
                     /gene="kduD"
                     /locus_tag="VFZ80_00330"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HEX6285904.1"
                     /translation="MAHIIDLSGKTALVTGARRGLGKAIAVGLAEAGADVIGVSAAQE
                     ETGSEVEHLIERVGRSFIGRKCDLSDRRALYELLAWLTDERRRVDILVNNAGMISRHP
                     VAEHGDEEWDRVLEVNLSSPFILARELGRQMVERRSGKIVFTASLLSFQGGITVPSYT
                     ASKSAIAGLTKALANEWASHGVNVNAIAPGYMETDNTEAIRHDPVRSQAILDRIPAGR
                     WGLAEDIAGAAVFLASGSADYIHGTVLTVDGGWMGR"
     gene            complement(1352..2104)
                     /locus_tag="VFZ80_00335"
     CDS             complement(1352..2104)
                     /locus_tag="VFZ80_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX6285905.1"
                     /translation="MTSPGPGTPAPAVLRAAAILDVISKHADTYPRVSDIARETGIPH
                     SSVTNILAALVETGLVRRAGSGYSIGPTTVELASVFLRSDDTVQRFREFVPEMETLSA
                     ETVQLGTLVEGDVLFLARHDGAQPIALTSAIGKRLPASSTAIGKAMLARLDPSELAAT
                     LSDPLPQLTDRSHRDLAALSDDLAEIRDRGHATDNEEAAPNVVCLAVAVDTPEGDPRC
                     AISTTLFKDRLTPELRDRLVADLTAVASYLAK"
     gene            complement(2101..>2599)
                     /locus_tag="VFZ80_00340"
     CDS             complement(2101..>2599)
                     /locus_tag="VFZ80_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6285906.1"
                     /translation="GILIALVAGHAGADVLVSEVSPFRRNLATELGLRNIDPATEDLN
                     ATVSAWSDGKGADVAFEVSGSAAGVRALTDVLAVRGHGVVVAIHTEPTPVDLMAAFWK
                     ELDISGARVYQRDDFEAAVALLADGVIPAQSLITEVVPLSEADRAFDRIESGGEVVKV
                     MIDSR"
BASE COUNT          451 a          813 c          868 g          467 t
ORIGIN      
        1 ggcttgcgca gaaacagcct ggccacgtcg ctgtgtgggg gatccccgtc cggccacacc
       61 ttgtccagtg tgatgaacag gtcagagtac gccgacttgc ggccacgctg tacgaaatcc
      121 tggaactgcg ggctctgtcg ggatatgtgg ttgaccatga cgtcgagcag gacgtcgtgg
      181 ttggcggcga tctgttcgat gtccgcccag gtcccgaaga gcgggtcgat ctcgtcatat
      241 cggatggggg agaaccctcg atcacccgat gatgggaatg gcggcaggat gtggacgccc
      301 cgaaacaacc ctgccaatgg tccctgcatc agttccgaga ggctcttcaa atctccgccc
      361 agcgaatccg gataggtgat cagctgggga cggggggcga tcgggttgac gtgctggtcg
      421 gtggccaagt gtgtgatcag atattgagac aacgaccgag ggcgtctgtc aactcacggg
      481 cacgttggcc agcctttcca agagtcggag gtagtcgccg ttcaggatcc tcctggaaca
      541 gatcgcgatc ctaggagccc tctgctttcg ttggagatca gcgacccatc cacccgccgt
      601 cgaccgtgag cacggtgccg tggatgtagt cggccgatcc agacgccaag aagaccgcgg
      661 cgccggctat gtcttcggct agaccccagc gccccgccgg gattctgtcc agtatcgcct
      721 gcgacctgac cgggtcgtgg cggatagcct cggtgttgtc ggtctccatg tagccggggg
      781 cgatggcgtt gacattcact ccgtgacttg cccattcgtt ggccagagcc ttggtcaacc
      841 cggcgatcgc gctctttgac gccgtgtaac tcggaaccgt gattcctccc tggaagctga
      901 ggagagacgc ggtgaagacg atcttccccg accgccgctc gaccatctgt ctgccgagct
      961 cgcgggccag tatgaacggg gagctcaggt tgacctcgag cacgcggtcc cactcctcgt
     1021 cgccgtgctc ggcgacgggg tgtcgggaga tcatgcccgc gttgttcacc aggatgtcca
     1081 cccggcgccg ttcgtctgtc agccatgcca gcagctcgta gagcgcccga cgatccgaga
     1141 gatcgcactt tcgtccgatg aaggaccgcc ccacccgctc gatgagatgc tcgacctcgg
     1201 agcctgtctc ctcctgtgcg gccgagacgc cgatcacatc ggcgccggct tcggcgagcc
     1261 cgactgcgat cgccttgccg agcccccgtc tcgctcccgt caccagggcc gtctttccac
     1321 tcaggtcgat gatgtgggcc aagggtctcg gctacttggc cagatacgag gcgacggcgg
     1381 tcaggtcggc caccagccgg tcacgaagct cgggtgtcag gcgatccttg aacagtgtgg
     1441 tgctgatcgc gcatctggga tcgccctccg gcgtgtcgac ggcgaccgcg aggcaaacga
     1501 cgttgggcgc cgcttcctcg ttgtccgtgg cgtgaccgcg gtctcggatc tcggcgaggt
     1561 catcagagag tgcggccagg tcgcggtgcg agcgatcggt gagctggggg agaggatcgc
     1621 tcaacgtcgc cgccagctcg gatgggtcca gccgagccag catggctttc ccgatggccg
     1681 tggacgaagc agggagtcgc ttgccgatgg ccgaggtcaa ggcgatcggc tgggcgccgt
     1741 cgtgacgagc gagaaagagg acatcaccct cgacgagtgt tcccaattgg accgtctcgg
     1801 ccgagagggt ctccatctcc gggacgaact cccggaatcg ttgcaccgtg tcgtcgctgc
     1861 gcaggaacac cgaggcgagc tcgacggtgg tcggacctat cgagtatccc gaaccggcgc
     1921 gtcttacgag accggtttcc accagggcgg cgaggatgtt ggtcaccgac gagtggggta
     1981 tgcccgtctc ccgtgcgatg tccgacaccc gcggataggt gtctgcatgc ttcgagatca
     2041 catcgaggat ggcggcagcg cggaggactg cgggtgcggg cgtgccggga cctggggacg
     2101 tcatcgtgaa tcgatcatga ctttcacgac ctctccaccg gactcgattc ggtcgaaggc
     2161 gcgatcggcc tccgacaggg ggacgacctc ggtgatcaga gactgtgcag gaatgacccc
     2221 gtctgcgagg agcgcaacgg ccgcctcgaa gtcatcccgc tgatagaccc gggcaccact
     2281 gatgtcgagc tccttccaga acgcggccat caggtcgacc ggggtgggct cggtgtggat
     2341 ggcgaccacg acgccgtgtc cacgcacggc cagcacgtcg gtgagtgccc ggaccccagc
     2401 cgccgacccg gagacctcga aggcgacatc cgcgcctttc ccatccgacc aagcggacac
     2461 agttgcgttc agatcctcgg tggcggggtc gatattgcgc aggcccaatt cggtggcgag
     2521 gttacgtcgg aacggcgaga cctcggacac gaggacatcg gcgccggcgt ggcccgcaac
     2581 gagcgcgatc aagatcccg
//
LOCUS       DASUTA010000018         3341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1691434, whole genome shotgun sequence.
ACCESSION   DASUTA010000018 DASUTA010000000
VERSION     DASUTA010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691434"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VFZ80_00345"
     CDS             complement(<1..276)
                     /locus_tag="VFZ80_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285907.1"
                     /translation="MIAVPYFGVGLLVTLATFTVADAPDWGVWLLFTALYVLLELLTV
                     EVNDRMFQSSSIMVLMTAGVIFATQTSSDAVFALALMAGLGATVPDDF"
     gene            831..1451
                     /locus_tag="VFZ80_00350"
     CDS             831..1451
                     /locus_tag="VFZ80_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285908.1"
                     /translation="MDVADLQEKIARIDGVEAARVVAGNGHIDEIHVLARRTKPPKQL
                     VRDVQSLGQALFGLDIDRRIVSVVQLADSVLASGSRPALADVAESVDGNRIEVTVTLK
                     WKERLLVGQATGAAAVATRWRHIAEATLEAVRQAIHSDIGLGVSSMDITTLGSRRLAI
                     SQVVVVTESGERLLIGSAYVEDDESRAVVRSVLDALNRLLPDLKTP"
     gene            1448..2998
                     /locus_tag="VFZ80_00355"
     CDS             1448..2998
                     /locus_tag="VFZ80_00355"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX6285909.1"
                     /translation="MTANAVVTPHHLSAAAGRDILEAGGNAVDAAVAAIAAQGVVAPE
                     TCGIGGDLFALIHAPGWEKPRALNSSGRAGSGVAAAELRSSGAGSIPADHPAAVTIPG
                     CVDGMETLIAELGRLTLKQVLAPAIGLARDGFAVSTEQERAFSRQADVYSANPAVSSF
                     YPNGEPVRRGDIVVRGDLAGTLADIAENGRSAFYEGQPATDIVESLGGSITFEDMRSS
                     QATWIEPIGTRVAASTAWTIPPNSQGYLGPATLAVFEMLDPPGDPDDPLRWHLLIEAY
                     RCLAWERDDLVADSDHAALPADLLLDKDRLERAAASVERERTGVWPRIGPSASTAYLC
                     AADADGLAVSMIQSNYRGTGSPFGAARSGFLLQDRGMGFTLMPGHPNELAPGKRPLHT
                     LSPTLWTEHDRPRWILGTRGGSVQPQLIAQMASSAILSGEDLNRAQARPRWTVSEFGP
                     GTGSGPTLEPGFHAAGVADLEARGHDLTVLAGPQPGWGPISIIELDGEIRRAVADPRV
                     DTTSALIF"
     gene            complement(3062..>3341)
                     /locus_tag="VFZ80_00360"
     CDS             complement(3062..>3341)
                     /locus_tag="VFZ80_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285910.1"
                     /translation="TPSYRSVLEASGWDYGEKLTAMSKRGEWDKMPAAVPDSALEGAG
                     VIAPIDGLAKAIKDRYGDRVQRIGFYSLGSVLMDDHDALKQVIADLQR"
BASE COUNT          638 a         1066 c         1049 g          588 t
ORIGIN      
        1 gaagtcatcc gggactgtcg ctccgagacc agccatgagg gctagtgcaa aaactgcgtc
       61 ggagctcgtc tgagtcgcaa agatgacacc ggctgtcatc aacaccatga tcgaggaaga
      121 ttggaacatg cgatcgttga cctcgacggt caacaactcc aataagacgt agagcgccgt
      181 gaataggagc cagacacccc agtcgggagc atctgcgacg gtgaaggtgg caagggtcac
      241 cagaagaccc acgccaaaat agggcacagc gatcatcagt tttcgctgca ccgctggctg
      301 gagacgccag tagcgactcc gcaagctatc caatccaagc aacttcaaag gggaccccgg
      361 ttgtgcctcc gactcaggtg agatggcgcc tctattcagc cgatcttcgt cgttgccgag
      421 ccagctgcaa tcaaagcggc aaggctggcg gcggcagcga gtattcggac caaacgggtt
      481 ctcataccta ttgcacgtcc acctgcagtt tctttatttg caccagcgtt gtgtgccggc
      541 acagaaagtt ccgctccgct cgtgtcgatg ggcgtgtact tacaagtgcg actgaactga
      601 ggacgccgtt ctcaccgtgc tcccgaatat cgggaaccct ttacatgtca aaaggcccct
      661 gacttcgccc gccaaaggga tcagcaagcg gtcatgagac cgttgcccca cgtgcccatt
      721 ggattttcct tccagccgtg cgcaatctaa acggatgcga gagatgccac aagagaaaag
      781 gcgaaatggc tctatcagcg cgtcggggcg cggtatacgc tgagaacagg atggatgtag
      841 ccgacctcca agagaagatc gccaggatcg atggggtgga ggccgcgaga gtggtggcgg
      901 ggaacggcca catcgacgag atccatgtcc ttgcccgtcg caccaaaccg ccgaaacagc
      961 tggtcagaga tgttcagtct ctgggtcagg cactgttcgg gctcgacatc gatcgacgaa
     1021 tcgtctcggt ggtccagttg gccgactcgg ttctggccag cgggagccgg cccgccctgg
     1081 ccgacgtagc cgagtcggta gacggaaacc gcatcgaggt cacggtcacg ctcaaatgga
     1141 aagagcggct gctcgtaggg caggcgacag gagctgccgc ggtggcgacg aggtggcggc
     1201 atatcgctga ggcgacactc gaggcggtcc gccaggcgat tcacagcgac atcggtctcg
     1261 gcgtcagctc gatggatatc acgactctgg ggagccgacg tctggcaatc tcccaggtgg
     1321 tcgtggtgac cgagtccgga gagcgactcc tcatcggatc ggcctatgtc gaagacgacg
     1381 agtcgagagc ggtcgttcgc tcggtgctcg acgcgttgaa tcgtctgctt cccgacctga
     1441 agaccccttg actgcgaacg cagtcgtcac accgcaccac ctgtccgctg cggcgggccg
     1501 ggacatcctc gaggccgggg gcaacgcagt ggatgcggcc gtggccgcaa tcgccgccca
     1561 gggtgtcgta gctccagaga cctgcgggat cggtggtgat ctcttcgctc tgattcatgc
     1621 tcccggctgg gagaagcctc gagcattgaa ctcatcgggg cgggccggtt ccggagtggc
     1681 cgccgccgag ctccgttcct cgggtgccgg gtcgatcccc gccgaccatc cggcagcagt
     1741 caccatcccc ggctgtgtcg acggcatgga gaccctcatc gccgagctcg gacggttgac
     1801 cctgaaacaa gtgctggctc ctgcgatcgg cttggcacgt gacggctttg cggtgtcgac
     1861 ggaacaggag cgggccttct cccgacaggc cgacgtctac tcggccaacc ccgccgtttc
     1921 atcgttctat ccaaacggtg aaccggtgag gagaggcgac atcgtcgtca gaggggacct
     1981 ggctgggacg ctggccgaca tcgccgagaa cggtcgttcc gccttctacg aaggtcagcc
     2041 ggcgacagac atcgtcgaat cgctcggggg ctcgatcacc ttcgaagaca tgagaagcag
     2101 ccaggcaacc tggatcgagc caattgggac aagagtcgca gcatcgacag cgtggacgat
     2161 cccgcccaac tctcaggggt acctcggccc cgcgacccta gccgtgttcg agatgctcga
     2221 ccctccgggt gatcctgacg acccgttgcg gtggcacctc ctgatcgagg cttaccgctg
     2281 tctcgcctgg gagcgagacg acctggtcgc cgactccgat cacgccgctc tccccgccga
     2341 tctcctgctc gacaaggacc gactggagcg tgccgcggcg agtgtcgagc gagaacggac
     2401 cggggtgtgg ccacgtattg gcccttcagc gagcacggca tatctctgtg ccgccgatgc
     2461 cgatgggctg gccgtgtcga tgatccagtc gaactatcgg gggacggggt cgcccttcgg
     2521 cgccgctcgc tctggattcc tcctccagga tcgcgggatg gggttcacgc tcatgccggg
     2581 ccatccgaac gagctcgcgc ccgggaagcg acctctccac acactctcac cgactctctg
     2641 gactgagcat gatcgaccga gatggattct ggggaccaga ggtgggtcgg tccagcccca
     2701 actcatcgca cagatggcgt cgtcggccat cctgtcgggg gaggatctca atcgggctca
     2761 ggcaagaccg cgctggaccg tctccgaatt cgggcccggg accggatcgg ggccaacact
     2821 cgagccggga tttcacgccg cgggagtggc cgacctcgag gccagagggc acgacctcac
     2881 ggttctggcc ggcccccagc ccggatgggg cccgatctcg atcatcgagc tcgacggcga
     2941 aatccgccga gcggtggccg acccccgtgt cgacacgacc agtgccctga tcttctgacc
     3001 acctgcaacc ccgaacccgg ggttgcgcgg aatccccgct ccaccccgag ttgagcgggg
     3061 attagcgctg gaggtcggcg atgacctgtt tcagcgcgtc gtggtcgtcc atgaggaccg
     3121 agcccaggct gtagaaaccg atccgctgca cccggtcgcc gtatctgtcc ttgatcgctt
     3181 tggccagccc atcgatggga gcgatgacac cggcgccctc caacgccgag tcggggaccg
     3241 ccgcaggcat cttgtcccac tcccctctct tcgacatcgc ggtgagcttc tccccgtagt
     3301 cccacccgct cgcctccaac accgatcgat aggacggtgt c
//
LOCUS       DASUTA010000019         3261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1821454, whole genome shotgun sequence.
ACCESSION   DASUTA010000019 DASUTA010000000
VERSION     DASUTA010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1821454"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VFZ80_00365"
     CDS             complement(<1..861)
                     /locus_tag="VFZ80_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006062269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6285911.1"
                     /translation="MTDAAILTEGLTKHYGDVKALVDLDLEVKMGEVFGFLGPNGAGK
                     TTMIRSCLDLIHPTAGRATLVGLDSRTDSVEIRKHIGYLPSDLSMYGDLTGRDMITYF
                     ANLRGGVDRSYVDELSDRLQADLGKKVGDLSSGNRQKVGLILAFMSRPQLIIMDEPST
                     GLDPLVQREFQAMMREVATDGRCVFLSSHTLSEVQRVADRVGIIRTGHLVDVESVDDL
                     RSKGIRTVELHFDSPVDASVFESVNGVRDVMVADRHASMSFDGQMDALLRVATAHYTL
                     LDVNTQEADLE"
     gene            complement(858..1325)
                     /locus_tag="VFZ80_00370"
     CDS             complement(858..1325)
                     /locus_tag="VFZ80_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX6285912.1"
                     /translation="MKPSPDQHEFVEHMGRWWETGTGSRAAGRILGWLMICDPTHRSA
                     GELVDELGLSSGSVSTQLRLLESLQFVERITFPGDRITYYQLKPDVWMTVMASEMDHL
                     DRMRRISEVGAGVVPTERPERVTDVGVIAEFFLDKWPSFMDELRAHIEKERQT"
     gene            complement(1420..2064)
                     /locus_tag="VFZ80_00375"
     CDS             complement(1420..2064)
                     /locus_tag="VFZ80_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX6285913.1"
                     /translation="MAECCDPAAYGRIFDSKEAERRLRRYRRKGLDRMAARLLGYLQG
                     RDLVGRTILEVGGGIGDFQVELLKSGAAGATNVELSGAYERTARELSDEENLTDRVER
                     RLGDFVEEQQSVEPADLVVLNRVICCYPWMDRLMEASVAKTNWLLAIAVPRDRLVSRI
                     MVKVGNAILRLRGQDFDAYVHSVAGMEAIAARAGLTRVFIRQGLVWEGLVFERL"
     gene            complement(2143..>3261)
                     /locus_tag="VFZ80_00380"
     CDS             complement(2143..>3261)
                     /locus_tag="VFZ80_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEX6285914.1"
                     /translation="VPIAMAAGLGINAAVAFGLVLTDGLTPEGAMGVIVLEGLIVTAL
                     VLVGMREAIMHAVPLALKRAIGVGIGLFILFIGFVSGGLIAGTGAPDAPVAFVFPNSN
                     AAWVTIVGLVITVILYARKVPGSLIISILATTVVALLWGVAEVPDSLSVTPSFDTLGA
                     FDLGNVFDQLGLVAALLTIFSFMLTDFFDTMGTATAITEQAGLTDEDGRIEDIGKLLF
                     VDSIGAAAGGAAGVSSNTSYIESSAGVAEGGRTGLTSIVVGILFLIAIFLSPLATIVP
                     PQATAPVLILVGFLMAGLIKGIDFDDLDEGFPALLAIILMPLTFSITVGIGAGFVMYT
                     LIKVVKGKAGEVHPLMWVVTLAFLVYFGQEVLGSVISA"
BASE COUNT          669 a         1040 c          982 g          570 t
ORIGIN      
        1 ctcgagatca gcctcttggg tgttgacgtc gagcagcgtg taatgcgccg ttgccacccg
       61 gagcaaggca tccatctgcc cgtcgaaaga catcgaggcg tgacggtctg cgaccatcac
      121 atcgcggaca ccgttgactg actcgaagac agaggcgtcg accggtgagt cgaaatggag
      181 ctcgacggtg cggatcccct tcgacctcag gtcgtccacc gactccacat cgaccagatg
      241 tccggtgcgg atgattccga ctcggtcggc cactcgctgc acctcggaga gggtgtggct
      301 ggagagaaac acacatcggc catccgtggc gacttcgcgc atcatggcct gaaactcacg
      361 ctgcaccagg ggatcgaggc cggttgacgg ctcgtccatg atgatcagct gcggccggct
      421 catgaaggcc aggatgaggc ccaccttctg cctgttgcct gacgagaggt caccgacctt
      481 cttgcccagg tccgcctgga gtcgatccga tagctcatcg acataggagc gatcgacccc
      541 gccccgcagg ttggcgaagt aggtgatcat gtcgcggccg gtcaggtctc cgtacatcga
      601 cagatccgag ggcagatatc cgatgtgctt tctgatctca acagagtcgg tccgcgaatc
      661 gagaccgacc aaggtcgccc ggcccgcggt cgggtggatc agatcgagac atgatctgat
      721 catggtggtc ttgccggcgc cgttgggccc gaggaaaccg aaaacctccc ccatcttcac
      781 ctcgaggtcg agatcgacca gcgccttaac gtctccgtag tgcttggtga gaccttccgt
      841 caggatcgcc gcatcggtca tgtctgtctc tctttctcga tatgagccct cagctcatcc
      901 atgaaggatg gccatttgtc caagaagaac tcggcgatga cgccaacatc ggtgacccgc
      961 tccgggcgct ctgtgggcac cacgcccgcc ccgacctcgg aaatcctcct catccggtcg
     1021 aggtggtcca tctccgaagc catgaccgtc atccacacat cgggtttcag ctggtagtag
     1081 gtgattcggt caccgggaaa cgtgattctc tccacgaact gaaggctctc cagcaggcgt
     1141 agctgggtcg agacggaccc cgagctcaga ccgagctcgt cgacgagctc accagccgac
     1201 cgatgcgtgg ggtcgcagat catcagccag ccgagaattc tccccgcggc gcgactgccg
     1261 gtgccagtct cccaccagcg tcccatgtgc tcgacgaact catgttgatc gggcgacggt
     1321 ttcattcgtt tcaacgttag ctgaaaccac tgaagcttgc cagggtgttg gaataagcat
     1381 caggcggaaa ccactcatct tgctatagca acgccgagat cagagacgtt cgaagacgag
     1441 cccttcccag acgagcccct gccggatgaa cactcgggtc aggccggcgc gggcggcgat
     1501 ggcctccatg ccggccacgg agtggacgta ggcgtcgaaa tcctgacctc gcaggcgaag
     1561 gatcgcattg cccactttca ccatgatgcg ggacacgaga cgatcacgag ggacggcgat
     1621 cgccagcagc cagttggtct tggcaactga ggcctccatc aagcgatcca tccagggata
     1681 gcagcagatg actcggttca gcactacgag atcggcgggc tcgactgatt gctgttcctc
     1741 gacgaaatct ccaagacgac gctcgacacg atcggtgagg ttctcttcgt cgctgagctc
     1801 gcgggcagtt cgctcatacg cgccagacag ttcgacgttg gtcgctcccg ccgcacccga
     1861 cttgagcaac tcgacctgaa agtccccgat ccccccgccg acttcgagga tcgtcctgcc
     1921 caccagatcg cgcccctgga gatagccgag gagccgtgcc gccatccgat cgaggccttt
     1981 gcggcgataa cgcctgagcc ggcgttccgc ttctttgctg tcgaatatgc gtccataggc
     2041 agcgggatca cagcattcgg ccatgccgga gagactaagc ccgtgaaagg agggagggac
     2101 cggcgccggt ccctccctcg atggcccctg tagccaactc tgtcaggcgg agatcacgct
     2161 tcccaacacc tcctgaccga aatagaccag gaatgccagc gtgaccaccc acatcaacgg
     2221 gtggacctcg cccgccttgc ccttgaccac cttgatcagc gtgtacatca cgaaaccggc
     2281 accgatcccg accgtgatgc tgaaagtgag aggcatcagg atgatggcga gaagggcggg
     2341 gaagccctca tcgaggtcat cgaagtcgat gcccttgatc aaaccggcca tgagaaaccc
     2401 gaccagtatc agcaccggtg ccgtggcctg gggcgggacg atggtggcca gcggggagag
     2461 gaagatcgcg atcaggaaca gaatcccgac cacgatcgac gtcagcccgg tccgaccacc
     2521 ttcggcgaca cccgccgagc tctcgatgta gctggtgttg gagctgactc cggcggcacc
     2581 tccggccgcc gcgccgatgg aatcgacgaa cagcagtttg ccgatgtcct cgatgcgacc
     2641 gtcttcgtcg gtgagaccgg cctgttcggt gatggccgtc gccgtgccca tggtgtcgaa
     2701 gaagtcggtc agcatgaagc tgaagatcgt gagcagagcc gccaccagac ccaactggtc
     2761 gaacacgttt ccgagatcga atgcgcccag agtgtcgaag ctgggcgtca ccgacaagct
     2821 gtctggcacc tcggccacgc cccagagcaa agcgaccacg gtcgtcgcca ggatgctgat
     2881 gatcagtgag cccggcacct tcctcgcgta gagaatcacc gtgatcacca gcccgacgat
     2941 ggtgacccag gcggcgttgg agttggggaa cacaaacgcg accggagcat cgggggctcc
     3001 cgtgccggcg atgagaccgc cgctgacgaa gccgatgaac aggatgaaca gcccgatgcc
     3061 gacaccgatg gctctcttca aagccaaggg cacggcgtgc atgatcgcct ctcgcatgcc
     3121 caccagcacg agggcggtga cgatgaggcc ttccagcacg atgacaccca ttgctccttc
     3181 gggggtcagc ccgtcggtga ggacgagccc aaaggcgacg gccgcgttga tgccgagccc
     3241 ggccgccatg gcgatcggga c
//
LOCUS       DASUTA010000020         3849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1433303, whole genome shotgun sequence.
ACCESSION   DASUTA010000020 DASUTA010000000
VERSION     DASUTA010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433303"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1658)
                     /locus_tag="VFZ80_00385"
     CDS             complement(<1..1658)
                     /locus_tag="VFZ80_00385"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX6285915.1"
                     /translation="MTCATCAVRVERVLGRQEGVERASVNLAGASASVRVSDDADVDA
                     LASAVDKIGYGLTIHEGDQGRDTVTMYSEEERQQLRRFWVALAFTAPAMLLHLLGPHA
                     MWNAVIQGILVTPVVFWAGAQYHRRALKQARVGAANMDTLISLGSLAAYFYSLAVLFV
                     GGAVFFETAGMIITLITLGKVFEARAKGRASSAVHRLLELGASEATVITESGERRVPV
                     EQVIPGDLMVVRPGERIPTDGVVETGSSTVDESMLTGEPLPVTKREGDEVVGATVNQA
                     GRLVVRATRVGSDTTLASIIRMVEEAQGSKAPIQRLADVYSARFVAGVLLITLAVFGF
                     WLLAGDSVGTAVRIAVSVVIIACPCALGLATPTAVMVGSGRGAELGILYKQAEVFERA
                     REIGTVLFDKTGTLTTGVMTPQLIDTQEDRRTFLRLVGSIEVASGHPIGIAVGLAADE
                     EDVELTEPTDLETIPGLGVSGTVDGHLVFAGTGDLVEERGLSGMDRWAETLNRAAAAG
                     QTAFYAGWDGLVRGVISVSDSIRPSAAAAVARLEALGIESELVTG"
     gene            complement(1705..1917)
                     /locus_tag="VFZ80_00390"
     CDS             complement(1705..1917)
                     /locus_tag="VFZ80_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004432155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HEX6285916.1"
                     /translation="MTTQKTLQVPEVHCDHCKTSLEGAVGALNGVSTVEVDVPNATIE
                     VDYDDDAVSLDTIKTTIEEQGYAVFG"
     gene            complement(1914..2318)
                     /locus_tag="VFZ80_00395"
     CDS             complement(1914..2318)
                     /locus_tag="VFZ80_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HEX6285917.1"
                     /translation="MKIGQLAERTGVSTKAIRYYENIGVLPPARREANGYRVYDKAAA
                     ERIGFIQDAQSAGMSLLEIQMILGLRDDGVSTCGHVIATLETHRAEIGRQMEDLERTR
                     RRLDEIIESARALDPADCDDPNRCQTIPKGTM"
     gene            complement(2439..2633)
                     /locus_tag="VFZ80_00400"
     CDS             complement(2439..2633)
                     /locus_tag="VFZ80_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285918.1"
                     /translation="MTDTVRGAAIKGMTRPSKRFATGRVCAKKGCETKLSQYNKAEYC
                     HAHAPIKFPRVRGKIVDGEE"
     gene            complement(2800..>3849)
                     /locus_tag="VFZ80_00405"
     CDS             complement(2800..>3849)
                     /locus_tag="VFZ80_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX6285919.1"
                     /translation="RVEQWDWSYYDTVQRKTDYGVDNFEVAKYFPLPQVLEGMFELTS
                     EMFGITFTEIDAFDSWHPDVQLFAIQDTDTGEEISRFYLDLFPREGKFGHAAEFPLIR
                     SRLLEDGSYQNPLCAMVANLTKPTKDSPSLLQHSEVETLFHEFGHVLHQNLGRTELAT
                     FSGTQTERDFVEAPSQIMEHWIWRSDVLRRFARHYETGEPIPTELVDQLVAARRLNKA
                     MWQLRQMQYGWWDQELHAGPDRDIDSIHAAGSEISMLPPHEGTFALASFGHIMGGYDA
                     SYYGYMWAEVFGDDMFSRFENEGVTNPEVGRAYRQAVLSRGGALDADDMLVDFLGREP
                     NNEAFLRKLGISGTG"
BASE COUNT          703 a         1196 c         1211 g          739 t
ORIGIN      
        1 ccggttacga gctcagactc gatgccgaga gcctccaagc gcgccactgc tgccgcggcc
       61 gaaggtcgga tggagtcgga gacggagatg actccccgca ccaaaccgtc ccatccggcg
      121 tagaaggctg tctggccggc agcggcggcc cgattcagag tctccgccca tctgtccatc
      181 ccgctgagcc cgcgctcctc gacgagatca ccggtacctg cgaagacgag atgcccgtcg
      241 acggtcccgg agaccccgag gcccgggatc gtctccagat cagttggctc cgtgagttcg
      301 acgtcctcct cgtcggcggc caacccaaca gcgatgccga tgggatggcc tgaggcgacc
      361 tcgatggagc cgaccagtct caggaaagtc cgacgatcct cttgggtgtc gatcagttga
      421 ggagtcatga cgcccgtggt gagcgtcccg gtcttgtcga agaggacggt cccgatctcc
      481 cgggcacgct cgaagacttc tgcctgcttg tagaggatgc cgagctcggc gccgcgaccg
      541 gagccgacca tcactgcggt cggggtggcc agcccgagag cacagggaca ggcgatgatc
      601 accactgata ccgcgatgcg gacggcggtt cccaccgaat cacctgcgag gagccagaac
      661 ccgaacacgg cgagggtgat cagcaggacg ccggctacga atctcgccga gtagacgtcg
      721 gcgagccgtt ggatcggcgc cttagagcct tgcgcctcct cgaccatgcg gatgatcgag
      781 gcgagcgtgg tgtccgaacc cacgcgtgtc gctcggacga cgagacgccc tgcttggttg
      841 actgtggcgc cgaccacctc gtcgccttct ctcttggtaa cgggaagagg ctcaccggtg
      901 agcatcgact cgtcgacagt gctggaaccg gtctcgacga cgccatcggt tgggatgcgc
      961 tcaccgggtc tcaccaccat caggtcaccg gggatcacct gttcgacggg gacacgtctc
     1021 tcgcctgatt cggtgatcac ggtggcctca ctcgctccta gctcgaggag ccgatgaacc
     1081 gccgacgaag cacgtccttt tgccctggcc tcgaacacct tgcccagcgt gatgagtgtg
     1141 atgatcatcc ccgccgtctc gaagaagacc gcgccaccca cgaagaggac ggcgagcgag
     1201 tagaagtagg cggccaggga accaagcgag atcaaggtat ccatgttggc ggcaccgacc
     1261 cgggcctgtt tcagggcacg ccggtgatat tgagcgccgg cccagaacac gaccggtgtc
     1321 accaggatcc cctggatcac cgcgttccac atggcgtggg ggcccaacag gtgcaggagc
     1381 atcgccggag ccgtgaaggc gagagcaacc cagaagcggc ggagctgttg cctctcttcc
     1441 tctgaataca tggtcaccgt gtcgcggccc tggtctcctt cgtggatggt gaggccgtac
     1501 ccaatcttgt cgacggccga ggcaagggcg tcgacatcgg catcgtcgga gacccgaacc
     1561 gatgccgaag cgccggccag attcacagaa gccctctcca ccccctcctg gcggcccaga
     1621 actcgctcga ctcgaactgc acaagtcgca caggtcatcc cctcgacatc gaagaggagc
     1681 gtcctggtcg actccccgtg cttctcagcc gaagactgcg tagccctgct cctcgatcgt
     1741 ggtcttgatg gtgtccaggc tcaccgcgtc gtcgtcataa tcgacttcga tcgtggcgtt
     1801 ggggacatcc acctcgacgg tggacactcc gttgagagcg ccgacggcgc cctcgaggct
     1861 ggtcttgcag tggtcgcagt gcacctcggg cacctggagg gtcttctgag tggtcacatc
     1921 gttcctttcg ggatggtctg gcatcgattg gggtcgtcac agtcggccgg gtcgagggcg
     1981 cgggcactct caatgatctc gtcgaggcgg cgacgagtgc gctccaggtc ctccatctgc
     2041 cgtccgatct cggccctatg cgtctccagc gtggcaatca catgtccgca ggtcgacaca
     2101 ccgtcgtcgc gcaagccgag gatcatctgg atctcgagca gagacatccc cgcagattga
     2161 gcatcctgga tgaacccgat ccgctccgcg gccgccttgt catacacgcg atatccgttg
     2221 gcctccctac gggcgggagg gagaacgccg atgttctcgt agtaacggat cgccttggtg
     2281 gagaccccgg tccgctcggc cagctgtccg atcttcatcc gcccagtata acagcaaaac
     2341 cttccagttc actagaaggt ctgtccgaac ggttggaaac tgagaaggcc cccgacctgg
     2401 gcggtagggg gccttcgaat ggggttatcg gggtgcgatc actcttcgcc gtccacgatc
     2461 ttgccacgga cgcgtgggaa cttgatcggg gcgtgggcgt ggcaatactc ggccttgttg
     2521 tactggctga gcttggtctc acagcccttc ttggcgcaca cccgcccggt ggcaaatcgc
     2581 ttcgatgggc gtgtcatgcc cttgatcgct gcacctcgta ctgtgtctgt catgttttgg
     2641 ccctcctggt aattgggccg gcactgttga aggttcgggt tttcaggctt ctcgcctgac
     2701 ccggccaaga cctccttggt cttgatgttg cactcaaatt gtgcgtgccg gaagctaacg
     2761 ggggttaaag ccttgttgga tatagacgta ggaaccgcct cacccggttc ccgagatacc
     2821 cagctttctg aggaacgcct cgttgttggg ctctcttccc agaaagtcta caagcatgtc
     2881 gtctgcgtcc aaagccccac cacgggaaag taccgcctgg cggtacgccc gaccgacctc
     2941 gggattggtc actccctcgt tctcgaagcg ggagaacatg tcgtccccga agacctcggc
     3001 ccacatatac ccgtagtagc tggcgtcgta tccacccatg atgtgaccga aggaggccag
     3061 agcgaaggtg ccctcgtgag gtggcagcat ggagatctcc gagccggcgg cgtggatgga
     3121 gtcgatgtcg cggtcgggcc cggcatggag ctcctggtcc caccacccat actgcatctg
     3181 tcggagctgc cacatcgcct tgttgagacg acgggcggca acgagctggt cgacgagctc
     3241 ggtggggatg ggctcacccg tctcgtagtg gcgggcgaag cgtctcagaa catccgatcg
     3301 ccagatccag tgctccatga tctggctggg ggcctcgacg aagtcgcgct cggtctgggt
     3361 gcccgagaac gtggccagct cggtacgacc caggttctgg tgtaggacgt ggccgaactc
     3421 gtggaagagg gtctcgacct ctgagtgttg cagtagcgaa ggggagtcct tggtcggctt
     3481 ggtgaggttg gccaccattg cacacaaagg attctgatag ctgccgtctt ccagcagccg
     3541 gctgcgtatc aaggggaact cagcagcatg gccgaacttt ccctcacgtg ggaagagatc
     3601 gaggtagaaa cgggagatct cctcgccggt gtcggtgtct tggatggcga agagttggac
     3661 gtcagggtgc catgagtcga acgcgtcgat ctcggtgaat gtgatgccga acatctcgga
     3721 ggtgagctcg aacatgccct ccagcacctg ggggaggggg aagtacttgg ccacctcgaa
     3781 gttgtccacg ccgtagtcgg tctttcgctg aaccgtgtcg taataggacc aatcccactg
     3841 ttccacccg
//
LOCUS       DASUTA010000021         2801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_655989, whole genome shotgun sequence.
ACCESSION   DASUTA010000021 DASUTA010000000
VERSION     DASUTA010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655989"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1887
                     /gene="infB"
                     /locus_tag="VFZ80_00410"
     CDS             <1..1887
                     /gene="infB"
                     /locus_tag="VFZ80_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX6285920.1"
                     /translation="PEPEVVEPAAEVETEEKPPAEKPERTDSADVEADTTEVESATEE
                     VEIASITAGATVAEFAEAIGQSPGEVVKALLNRGIPAGAQQTMPEDLIDDIAESFGYI
                     VDVTEPEAPPAVEEQPEFDDADEDLTPRPPVVTVMGHVDHGKTTLLDTIRKANVVEGE
                     EGGITQHIGAYQVEVNDRKITFVDTPGHEAFTALRARGANITDIVVLVVAADDGVMPQ
                     TVEAISHAKAAGVKMIVAVNKMDLPGADPLRVRTELTNHGVVVEELGGDVPSVEVSAV
                     NGTGVDDLLEVIDLIAQLEDFRANAKPGATGVVVEAQLEVGRGPVATVIVQRGTLRQG
                     DAFVAGPIAGRARALMNDQGDRIDQATPSDPVQIMGWDDVPAAGDFFEVQKNDREARK
                     IADERQEELRQEAHVAPSARDRLQGLLDQLRSEDATLNVIVKADAQGSLEALRESIAK
                     IEREGGKIQIVHTSVGGISETDVTLAEVTEAVIVGFNVRPEPKARRAAEEAGVEIRTY
                     GVIYELLDELEQLLVGQLAPDQQEVVLGTAEVRAIFKVPRAGTIAGCYVLEGMVQRGA
                     KARLLRDGVVIHDGVISSLKRFKDDVREVQSGFECGMGFEDYNDIKEGDNIEVYTVRE
                     VART"
     gene            1912..2253
                     /gene="rbfA"
                     /locus_tag="VFZ80_00415"
     CDS             1912..2253
                     /gene="rbfA"
                     /locus_tag="VFZ80_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX6285921.1"
                     /translation="MSDRMMRVNSTLREVLAEEIERMSDSRLELVSVTAVDTAPNLRS
                     AVVYIDVLSDDAREPALTALRGAGKRLQAAIGAEVRMKYTPTLEFKIDPGVTGGERIE
                     QILRELRQEEE"
     gene            2250..>2801
                     /locus_tag="VFZ80_00420"
     CDS             2250..>2801
                     /locus_tag="VFZ80_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX6285922.1"
                     /translation="MTLDTGALARAAEVIAGADRIALACHIGPDGDALGSMLGLAVAA
                     ENAGKEVVASFGSPYLIPPSLGFLPIHLLVPPDEFPAAPEVMVVLDAGSSDRLAELGS
                     NASDAETLVVIDHHVTNEGFGDVAVVDPKSAATGELVYAILQRLGWEITPSVAQCLQT
                     ALVTDTGRFSYSNTTPRTLEIASE"
BASE COUNT          553 a          879 c          928 g          441 t
ORIGIN      
        1 cccgagcccg aggtcgtcga gcccgccgcc gaggtggaga ccgaggagaa gcctcccgcc
       61 gagaagcctg agcgaacgga ctccgccgat gtcgaagcgg acacgaccga ggtcgaatct
      121 gcgaccgagg aagtcgagat cgcctcgatc accgcgggag ccacggtggc cgagttcgcc
      181 gaggcgatcg gccaatcacc cggagaagtg gtcaaggcac tgctcaacag gggtatccct
      241 gccggcgctc aacagacgat gccggaggat ctcatcgacg acatcgctga gtctttcggc
      301 tacatcgtcg atgtcaccga gccggaggca ccgcccgccg tcgaagagca gcccgagttc
      361 gacgacgccg acgaggatct gacgccgcgg ccacccgtcg tcacggtcat gggtcatgtc
      421 gatcacggca agacgaccct tctcgacacg attcgcaagg ccaacgtggt cgaaggcgaa
      481 gagggcggca tcacccagca catcggggcc tatcaggtcg aggtcaacga ccgcaagatc
      541 acattcgtcg acacccctgg ccacgaggcc tttaccgctc ttcgagcccg cggggccaac
      601 atcaccgaca tcgtcgtcct ggtcgtggcc gctgacgacg gtgtcatgcc acagacggtc
      661 gaagccatct cccatgccaa ggcggccggc gtcaagatga tcgtcgccgt caacaagatg
      721 gacctgcccg gggcggaccc gctccgggtg cgcaccgagc tgaccaacca cggcgtcgtc
      781 gtagaagagc taggtggcga cgtcccatcg gtggaagtct ccgcggtcaa cgggacaggc
      841 gtggacgacc tcctcgaagt catcgacctc atcgcgcagc tcgaggactt cagggcgaac
      901 gccaagccag gtgcaaccgg agtggtggtc gaggctcagc tcgaggtcgg tagaggccct
      961 gtcgccactg tcatcgtcca gcgggggacg cttagacaag gcgacgcctt cgtggccggg
     1021 ccgatcgcgg gcagggcaag ggccctgatg aacgatcagg gcgaccgcat cgaccaggcg
     1081 acaccgtccg atcccgtcca gatcatgggt tgggacgacg ttccggcggc cggcgacttc
     1141 ttcgaggtgc agaagaacga ccgtgaggcc cgcaagatcg cagacgagcg ccaggaggag
     1201 cttcgccagg aggctcatgt cgcaccatcg gcccgtgacc gtctccaggg cctactcgac
     1261 caactccgct ccgaggacgc caccctgaac gtgatcgtca aagccgacgc ccagggatcg
     1321 ctcgaagccc tgcgcgagtc gatagccaag atcgagcgcg agggcggcaa gatccagatc
     1381 gtccacacct cagtcggtgg gatcagcgag accgatgtga ccctggccga ggtgaccgag
     1441 gcggtgatcg tcggcttcaa cgtccgcccc gagccgaagg cgagacgagc tgccgaggaa
     1501 gccggtgtcg agattcgcac ctacggcgtc atctacgagc ttctggacga gctggagcag
     1561 ctgctcgtcg gccagctcgc acccgaccag caagaagtgg ttctgggaac agcagaggtc
     1621 agggccatct tcaaggtccc ccgcgccggc accatcgccg gctgctatgt gctcgagggc
     1681 atggttcagc ggggcgccaa ggcccgtctc ctccgcgacg gcgtggtgat ccacgatggt
     1741 gtcatctcat ccctgaagcg attcaaagac gacgtccgag aggtccagtc cggcttcgag
     1801 tgcggtatgg gcttcgagga ctacaacgac atcaaagagg gcgacaacat cgaggtgtac
     1861 acggtccgcg aggtggcccg cacgtagtct gacctgggga gcgtgccggc gatgagcgac
     1921 cgtatgatga gggtcaattc gactctgcgt gaggtgctcg ccgaggagat cgagaggatg
     1981 agcgactccc ggctcgaact ggtgtcggtg accgcggtgg acacggcgcc caacctgagg
     2041 agtgcggtcg tctacatcga tgtgctgagc gatgacgcca gggaaccggc gctgaccgcc
     2101 cttaggggcg cgggcaaacg acttcaagcg gcgattggag cagaggttcg aatgaagtac
     2161 accccgaccc tggaattcaa gatcgatcct ggggtgaccg ggggagagcg aatcgagcag
     2221 atcctgcgag agttgcgcca ggaggaagag tgacgctcga cactggcgcc ctggctcgtg
     2281 ccgccgaagt cattgctgga gccgaccgta tcgccctcgc ctgtcatatc gggcccgacg
     2341 gtgacgccct gggctcgatg ttgggattgg cggttgcagc ggagaacgcc ggcaaggagg
     2401 tggtggcgag cttcggatca ccgtatctga taccgccgag cctcggattt ctgccgatcc
     2461 atctgctggt gcctccagat gagttccctg cggcccccga agtgatggtg gtactcgacg
     2521 caggatcgtc ggaccgtctc gccgagctgg gcagcaatgc gtcggacgcg gagaccctcg
     2581 ttgtgatcga tcaccacgtc accaacgaag gattcgggga tgtcgcagtc gttgatccca
     2641 agtcggcggc caccggggag ctggtgtatg cgattctcca gcggctgggt tgggagatca
     2701 ccccgtctgt cgcccaatgc ctccagactg cactggtgac cgacaccggt cgtttcagct
     2761 attccaacac cacgcctcgc accctggaga tcgcctccga g
//
LOCUS       DASUTA010000022         2841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1045556, whole genome shotgun sequence.
ACCESSION   DASUTA010000022 DASUTA010000000
VERSION     DASUTA010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045556"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFZ80_00425"
     CDS             complement(<1..261)
                     /locus_tag="VFZ80_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285923.1"
                     /translation="MSTKRTTQEKAAIAIAAVASAWVIGAALFYSSGDINPAGILPTV
                     ASLIALGAAIDSNKTWMWVGAALALVFSVAFVFSVGLVVAPAA"
     gene            complement(293..1093)
                     /locus_tag="VFZ80_00430"
     CDS             complement(293..1093)
                     /locus_tag="VFZ80_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6285924.1"
                     /translation="MSESDRGQVAGSAAEVYEEFFVPALFGVWPRIVLEEAQVGAGHR
                     VLDVACGTGVLAREALVRVGETGEVVGLDPNAGMLAVARDREPAIDWEQGVAEDLPFE
                     SATFDRVVSQFGMMFFTDPEKAIEEMARVLSPNGRIAVAVWASLEETPGYAAMVDLLE
                     DLFGHRAADALRAPYVLGDPVAFEKLVGAVFPEVRVTCHEDVARFESIDAWVYTDVRG
                     WTLSEMIDDGQYAVLLDEAKRRLSRFRNEIGRVEFPAPALIATARAVT"
     gene            1163..1846
                     /locus_tag="VFZ80_00435"
     CDS             1163..1846
                     /locus_tag="VFZ80_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6285925.1"
                     /translation="MGTYRQYCPISRASEILAERWNPLIVRNLMFGADTFSKLARGVP
                     AMSRSMLIKRLQELERDGIIEREPKPDGHGHLYRLTDAGEDLGDVIDSLGVWGERWVE
                     VTTEHADPGFALWAWCQVQMNTAALPRDRTVVAFEFPQEPPGSRYYWLLVESGDAELC
                     YSDPGDEPDLYVTAESLAFVDWHRGRLDWPDALRSGRINVMGNRNMARGLPSWNTHEP
                     LVREETSSA"
     gene            complement(1852..>2841)
                     /locus_tag="VFZ80_00440"
     CDS             complement(1852..>2841)
                     /locus_tag="VFZ80_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX6285926.1"
                     /translation="DMLTISDRVIHAYSLGKERNHDAIEMIKIARQIDDSTLEREPSL
                     ITVVNASSPLRYDTPMLQGMIEFSSRNQVVVCTPFTLAGAMAPVTVAGALAQHNAEAL
                     AGIAFTQVVRPGAPVIYGGFTSNVDMQSGAPAFGTPEFMQTAMITGQLTRRYGIPYRS
                     SGVNAANALDAQAAYESVFSLWGAVMGGAHMILHAVGWMEGGLRASFEKMILDADLCA
                     MVARFMDPIDFSEGGLALDAVRDVGPGGHFFGTQHTLERYRDAFYAPLLSDWRNYEAW
                     EEAGRPEAKEKMIELVDTFMASYQKPPMDEAVREELEEYVERRKAEGGVPTDY"
BASE COUNT          521 a          911 c          891 g          518 t
ORIGIN      
        1 cgcagccggg gccactacca gcccaacgct gaaaacgaag gcgaccgaga agacgagggc
       61 gagagccgcc ccgacccaca tccacgtctt gttcgagtcg atcgccgccc cgagtgcgat
      121 gagggaggcg accgtcggca gtatccccgc cgggttgatg tcccctgacg aatagaacag
      181 ggcggcgcca atcacccatg ccgatgcaac tgcggcgatc gctatcgcag ccttctcctg
      241 agttgtgcgc tttgtcgaca agtgaacatc acccgggtca aagctagcga gctcaggtga
      301 cggccctcgc ggtggcgatc agagctggtg cggggaattc gaccctcccg atttcgtttc
      361 tgaatcgaga aagtcgtcgt tttgcctcgt cgagcaggac cgcatattga ccgtcgtcga
      421 tcatttcgga aagggtccag ccgcggacgt cggtgtagac ccacgcatcg atcgactcga
      481 atcgggcaac atcctcgtga cacgtcaccc tcacttcggg aaacaccgct ccgacgagtt
      541 tctcgaatgc cacggggtcg cccaacacat atggggccct cagcgcgtcg gcggccctat
      601 gaccgaacag gtcttcgagg aggtcgacca ttgcggcata tccgggcgtt tcctcgagag
      661 atgcccagac cgcaactgca atccgcccat tcggtgacag gacccgggcc atctcctcga
      721 tcgccttctc tgggtccgtg aagaacatca tcccgaattg gctcaccacc cgatcgaaag
      781 tggcggactc gaatgggaga tcttcggcca cgccttgctc ccagtcgatc gcaggttccc
      841 tatcccgggc caccgccagc atccccgcgt tggggtcgag gcccacgacc tcacccgtct
      901 caccgacccg gacgagcgcc tcccgagcga ggacacctgt gccgcaggcc acgtccagca
      961 cccggtggcc ggcaccgacc tgcgcttctt cgaggacgat cctgggccag acgccgaaca
     1021 gggcggggac gaagaactcc tcatagacct cggcggcgga gccagccact tgcccgcgat
     1081 cgctttcact catcctccaa tcgtcggtga cgcttctggt tccagctagc gcgaaatgag
     1141 aactggctag cctcgtgtgt ctatggggac gtatcgccaa tactgtccga tctcccgggc
     1201 atcggagatc ctcgccgagc ggtggaaccc gctcatagtg aggaatctca tgttcggtgc
     1261 cgatacgttc tcgaaactgg cgcgaggtgt cccggcgatg tccaggtcga tgttgatcaa
     1321 gcggctccag gagttggagc gtgacggcat catcgaacgg gagccgaagc ccgatgggca
     1381 cggccatcta taccgactca ccgacgcagg tgaggatctt ggagacgtga tcgacagcct
     1441 cggtgtctgg ggtgagcgat gggtcgaggt gacgaccgag cacgccgacc ccggattcgc
     1501 cctgtgggcg tggtgccagg tccaaatgaa tactgctgcg ttgccccggg accgaacggt
     1561 cgtcgcattc gagtttccgc aagagccgcc cggcagcagg tattactggc tcctggtgga
     1621 gagtggcgat gccgagctct gttactcgga tcccggagac gagcctgatc tctacgtcac
     1681 tgccgagtcg ctcgccttcg tcgattggca cagggggcgc ctcgattggc cggatgccct
     1741 ccggtcgggg cgaatcaatg tcatgggcaa tcggaatatg gctcggggcc tgccgtcctg
     1801 gaacacgcat gagccgctcg tgcgggaaga aacgagctcc gcctgaactg atcagtagtc
     1861 ggtcgggacc ccgccttccg cctttcgccg ctccacgtac tcctccaact cctctctcac
     1921 cgcttcgtcc atcggtggtt tctgatagga ggccatgaac gtgtcgacga gctcgatcat
     1981 cttctccttg gcctcgggtc gaccggcctc ctcccacgcc tcgtaattgc gccagtccga
     2041 gagcagaggg gcgtagaagg catcccggta gcgctccagg gtgtgctgtg tgccgaagaa
     2101 gtggccgccg gggccgacat cgcgcaccgc gtccagagcc aggcccccct cactgaagtc
     2161 gatcgggtcc atgaaccggg cgaccattgc acagaggtcg gcgtcgagga tcatcttctc
     2221 gaacgaggcg cggaggccgc cctccatcca tccgacggcg tggagaatca tgtgtgcccc
     2281 gcccatgacc gcgccccaca gcgagaagac cgactcatat gcggcctggg catcgagggc
     2341 gttggccgcg ttgacgccag acgacctgta tgggatgccg tagcgtcggg tcagctggcc
     2401 ggtgatcatc gccgtctgca tgaactcggg cgtcccgaag gcgggcgcac cgctctgcat
     2461 gtcgacgttc gaggtgaagc ctccatagat gacgggggcg ccgggacgaa ccacctgcgt
     2521 gaaagcgata ccggccagtg cctcggcgtt gtgctgcgcc agggcgccgg cgacggtcac
     2581 cggggccatg gcgccggcga gagtgaacgg agtgcagacg acgacctggt tcctcgagct
     2641 gaactcgatc atcccctgca gcatgggggt gtcgtatcgc aagggtgagc tggcgttgac
     2701 cacggtgatc aacgacggct cgcgctccag cgtggagtca tcgatttgac gcgcgatctt
     2761 gatcatctcg atggcgtcgt ggttgcgctc cttgcccagg ctgtatgcat ggatcacccg
     2821 gtcggagatc gtgagcatgt c
//
LOCUS       DASUTA010000023         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1305042, whole genome shotgun sequence.
ACCESSION   DASUTA010000023 DASUTA010000000
VERSION     DASUTA010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305042"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFZ80_00445"
     CDS             <1..207
                     /locus_tag="VFZ80_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285927.1"
                     /translation="SRTAHYLTEQTLDDGTSAAPTFEYYEFSGTSPLEEIRALADAAA
                     SHEPELTSVRDAIEGLSLILDRDR"
     gene            231..554
                     /locus_tag="VFZ80_00450"
     CDS             231..554
                     /locus_tag="VFZ80_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX6285928.1"
                     /translation="MTPDEVKGLLTRRRDTLAAELERLTEPPPATASVSFGKRVGDGT
                     TEAVERLTTTATARSVAASIAEIDRALQRLETGEYGTCESCGVAIPEARLEVRPAAST
                     CVNCA"
     gene            complement(575..1150)
                     /locus_tag="VFZ80_00455"
     CDS             complement(575..1150)
                     /locus_tag="VFZ80_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6285929.1"
                     /translation="MSHEHEIERWSPLKVWFYTLFHRHPKSNIAIVDYAQVSEGDRFL
                     DVGCGPGTALRQALRTGATAAGVDPSPSMVGRASKRVPEADVRVGSAEEIPFADDSFT
                     VVVNVASFHHWADRDGGLREIRRVLAPGGRLHVMEGKIGEGQDGHGLGPTEARLLTER
                     LVELGYSDARANTIKTGWRHEYYVVSATNPV"
     gene            1263..1739
                     /locus_tag="VFZ80_00460"
     CDS             1263..1739
                     /locus_tag="VFZ80_00460"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX6285930.1"
                     /translation="MTDTSTAEVRLPEDVPPKWANALMKWAITTPVLQSTIGQGVALL
                     TFTGHKTGETYKIPVSYQRDGDTVTIITKRARNWWRNFQTPAEVELRLAGRDYHGKAE
                     VFLDDEANLDFMTDYLAKRPIDAKAYGLAKSELARDKVARIIPHIVVIKIEIAADD"
     gene            1779..>2033
                     /locus_tag="VFZ80_00465"
     CDS             1779..>2033
                     /locus_tag="VFZ80_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285931.1"
                     /translation="MSELPVRDREAGSLRRWPRFLRRIVDFAVLPGDRPDLVRTKRLF
                     TAAMWVSIGTSLLSVYQLYVADVPWAAAAVSAAIVAAVISL"
BASE COUNT          373 a          654 c          626 g          380 t
ORIGIN      
        1 tcccgcaccg cccactacct caccgaacag acattggatg acgggacctc tgcggccccg
       61 accttcgagt actacgagtt ctcagggacc tcaccactgg aagagatccg tgccctggcc
      121 gacgccgctg cttcgcacga gcccgagttg acatcggtga gagatgcgat cgagggcctc
      181 agcctgatcc ttgatcgaga ccgatagctc ggtcgcccta ccctgaccga atgactcccg
      241 atgaggttaa gggcctcctt actcggcggc gtgacacatt ggcggcggaa ctggagcggc
      301 tcaccgaacc gccgccggcc acggcatcgg tctcgttcgg caaacgcgtg ggcgacggca
      361 ccaccgaggc ggtagagcgg ctgaccacca cggccactgc ccgttccgtt gccgcctcca
      421 tcgctgagat cgaccgcgct ctgcaacgtc tcgagaccgg cgagtacggg acctgcgagt
      481 cctgcggtgt ggcgattccg gaggctcgtc tcgaggtgag accggcagcc agcacctgcg
      541 tcaactgcgc ctgaacgtca gaccggtttg agcgtcagac aggattggtc gccgagacca
      601 cgtagtactc gtggcgccat cccgtcttga tggtgttggc gcgggcgtcg gaatagccca
      661 gttcgaccag cctttccgtc agcagccttg cctcggtggg gccgagcccg tgaccgtctt
      721 gaccttcgcc gatcttgcct tccatgacgt ggagacgtcc tccaggagcc aacactcggc
      781 ggatttctcg gaggcctccg tcgcggtcgg cccaatggtg gaaggacgca acgttgacta
      841 ccaccgtgaa cgagtcgtct gcgaaaggta tctcttcggc agaaccgacc ctgacatcgg
      901 cttccggtac acgcttggag gctcgcccca ccatcgaagg actgggatcg actccggcgg
      961 ccgtcgcccc cgttctcagc gcctgtctca gggcagttcc cggcccgcat cccacgtcga
     1021 ggaaccggtc accctcgctc acctgtgcgt agtcgacgat ggcgatgttg ctcttggggt
     1081 ggcgatgaaa gagtgtgtag aaccacacct tcagcggtga ccagcgctcg atctcgtgct
     1141 cgtgactcat cccggggcag gctagatgtc ggctgaacga tcgtgacttt cgactctggc
     1201 cgccttgctg ggcaggccgg atcatcgagg tgtcggatac gagacgagat tggaggctcg
     1261 agatgaccga cacatcgact gccgaggttc gcctccccga ggatgttcca cccaagtggg
     1321 cgaatgccct catgaagtgg gcgatcacca cgccggtgct gcaaagcacc atcggtcagg
     1381 gtgtcgcctt actcaccttc accgggcaca agaccggtga gacctacaag atcccggtga
     1441 gctatcaacg tgatggagac actgtcacca tcatcaccaa gcgggcacgc aactggtggc
     1501 gcaacttcca gacacccgcg gaggtggagc tgaggctggc gggacgtgac taccacggga
     1561 aggccgaggt attcctcgac gacgaggcca acctcgattt catgactgac tacctggcga
     1621 agcggcccat cgacgccaag gcatatgggc tggccaagtc ggagctggca cgggacaaag
     1681 tggcgaggat catcccgcac atcgtcgtga tcaagatcga gatcgctgcc gacgactgat
     1741 cgccctgttt ccgggctctg actaacttca ccgctcggat gagcgaactg ccggtcagag
     1801 acagggaggc cggttctctg cgtcgctggc cacggttttt gcggcgcata gtcgacttcg
     1861 cagtccttcc cggcgaccga ccggacctgg tcaggacgaa acgtctcttc actgcagcga
     1921 tgtgggtctc gatcggaacg tctcttctgt cggtctatca gctctatgtt gcggacgtcc
     1981 cttgggccgc ggccgcagtg agcgccgcga tcgtcgccgc ggtcatctcg ttg
//
LOCUS       DASUTA010000024         3243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1175404, whole genome shotgun sequence.
ACCESSION   DASUTA010000024 DASUTA010000000
VERSION     DASUTA010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175404"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="VFZ80_00470"
     CDS             <1..1031
                     /locus_tag="VFZ80_00470"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX6285932.1"
                     /translation="PVTVLATTTPGDQDLAVFDTSLQLGASEPLGTGVKRVTMEQLEF
                     AAAQFFDVELGLGGAVHESRKSIKRVRALLRLVRSQIPERIYTFENHSLRDTGRLISD
                     VRAAQGVVNAANSIHQLYSDLLAEGTFEELLANLQRRRDVAELAAAEDPNLVGRVVRG
                     LERAYHRYGSWPTDPEAQEVYGMGIRDSYEAVSPGLSKTYDRGRQGMVTAYRRGGPGD
                     FHEWRKRVKDMRHQMEFLAPLWPEVVVGTAMTLQRLGDLLGEDHDLDELGRLLRERPD
                     LCPNPRERSLFRALAGQRRSELQLASEILGRRIYAEKPKALAGRFGEYWQARRMAADG
                     SLDTLVVY"
     gene            1158..1901
                     /locus_tag="VFZ80_00475"
     CDS             1158..1901
                     /locus_tag="VFZ80_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX6285933.1"
                     /translation="MTDRGRVRIEPSQRRVRVMLAGEIVADTTSPLLVWEVPYYPTYY
                     FPEADVRTDLLVETGEVRKSPSRGEATQYAVKVNGSEGAAYSFREPKIDELGGHVAFV
                     WKTMDHWFEEDEEVFVHARDPYTRIDILPSSRRVRVEIDGVTVADTTNASFLFETGLP
                     TRYYLPKTDVRMDLLTPTDTSTSCPYKGTARYWSATVNGETHEDVVWGYDSPLPESQK
                     ITGLVSFYNEKVDVFVDEEHEERPKTKFS"
     gene            complement(1947..>3243)
                     /locus_tag="VFZ80_00480"
     CDS             complement(1947..>3243)
                     /locus_tag="VFZ80_00480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6285934.1"
                     /translation="HPGGGRREGEDTPGAVTTTADVVVVGAGIAGVSAAYHLAVGQGI
                     SSVVLVDPRPPLSLTSDKSTECYRNWWPDRAMVGLMNRSIDIYEQLAGETEFGFNHNG
                     YLFVTGEREKVTEMQRQARAIHPMGASADVLDRTALLDTYPFIAEEAAGALFVPRAGW
                     FRAQDLGLWMLDRARDAGATMIKASVDALVPGRVTLDDGSVISTGSTVIAAGPMSSEV
                     AELAGLDLPLFSELHLKVAFRDHLGIIPRDAPMTIWSDTQTIDWSEDEKAGLLDLGRD
                     DLIGEMPIFCHFRPEGGAESPYIVALWEYHDLVIEPTWPLPDDPLYPEVVLRGLTTMV
                     PGLAAYHDRLPESFVDGGYYTKTPENRPLIGPSGLDRVHLLTGMSGFGVMVSAGAADL
                     LAAHIAGKPLPDYADAFLLSRYDDPDYSANLANIESGQL"
BASE COUNT          621 a          992 c         1039 g          591 t
ORIGIN      
        1 gccccgtgac cgtcttggcc accacgacgc cgggcgatca ggacctggcg gttttcgaca
       61 cgagcctgca actcggtgcg tcagagcctc tgggtacggg ggtgaagcgg gtgaccatgg
      121 agcagctcga gttcgccgcc gcccagttct tcgatgtcga gctcggtctg gggggtgcgg
      181 tccacgaatc gcgcaagtcg atcaagaggg tgcgcgctct cttgcgtctg gtgcgaagtc
      241 agataccgga gaggatctac accttcgaga accattcgct acgcgatacc ggccgactca
      301 tatcagacgt tcgagctgca caaggagtcg tcaacgcagc caactccatt caccagctct
      361 acagcgacct cctcgccgag gggactttcg aggagctgct ggccaatctg caaagaagaa
      421 gagatgttgc cgagctcgct gcggcggaag accccaatct cgtagggcgt gtggtgcgtg
      481 ggttggagcg ggcctatcac cggtacgggt cgtggccgac cgacccggaa gctcaagagg
      541 tctacgggat gggcatcaga gactcttacg aggcggtgtc acccggactc tccaagacat
      601 acgaccgggg acgtcagggc atggtgaccg cgtatcgccg tggcggacca ggcgacttcc
      661 atgagtggcg caaacgtgtc aaggacatgc ggcatcagat ggagttcttg gcgccgttgt
      721 ggccggaagt cgttgttggc acggcgatga ccctgcagcg attgggagac cttctcggcg
      781 aggaccacga cctcgacgag ttggggcgac tgcttcgtga aagacccgat ctctgcccca
      841 atcctcggga gcgctctctg tttcgggccc tcgccggtca gcgccgatcg gaactgcagc
      901 tggccagcga gatcctcggt cgacgaatct acgccgagaa acccaaggcg ctggcaggtc
      961 gtttcggcga gtattggcag gcgcggcgta tggcggccga cggctctctc gacacactcg
     1021 tcgtctactg atcgaacacc cgcactgacg tgtaacgaac acgcctatac cgtgttcgtt
     1081 acacgtcagt caggtccttc ggaatcaacg actcctctgg cacgttcacc tcgttgcagc
     1141 cacatcagga gactcccatg acagatcgcg gccgagtgcg aatcgaaccg agccaacgac
     1201 gtgttcgggt gatgctcgca ggggagatcg tcgccgacac gacgagcccc ctcctcgtat
     1261 gggaagtccc ttactacccg acgtattact ttcccgaagc cgacgtcagg accgacctgc
     1321 tggtggagac gggtgaggtg cgaaagagcc cgagccgtgg cgaggcgacc cagtacgcgg
     1381 tcaaggtcaa cgggtcggag ggggcggctt actccttcag agagccgaag atcgacgagc
     1441 tcgggggaca cgttgccttc gtctggaaga cgatggacca ttggttcgaa gaggacgaag
     1501 aggtgttcgt ccatgcccgc gatccgtata cgcgcatcga catcctcccc tccagccgca
     1561 gggtgagagt ggagatcgac ggtgtgactg tggcggacac cacaaatgcc tcgtttctgt
     1621 tcgagaccgg cttgcccacc cgctactacc tgcccaaaac cgatgttcgc atggaccttc
     1681 tgacgcccac cgacacatct acgtcgtgtc cttacaaggg cactgctcgt tactggtcgg
     1741 cgacggtgaa cggtgagacc catgaggacg tcgtctgggg ttacgactct ccccttccgg
     1801 agtctcagaa gatcaccggg ctggtgagct tctacaacga gaaagtcgac gtcttcgtcg
     1861 acgaggagca cgaggagcgt ccgaagacga agttctcctg atgttcgatc gccccgggtt
     1921 cgaccggaat cctcgatcgg ctccgatcag agttggcccg attcgatgtt cgccagattc
     1981 gccgagtagt cggggtcgtc gtaacggctg agaaggaagg cgtcggcata gtccggcagc
     2041 ggtttgcctg cgatgtgggc ggccaggagg tcagcggccc cggcggacac catcaccccg
     2101 aaacccgaca tcccggtgag caggtgcact ctgtccaaac cagaaggtcc gatcaagggc
     2161 cggttctcgg gggtcttcgt gtaataccca ccatccacga acgactccgg cagccggtcg
     2221 tggtacgcgg ccagcccggg gaccatcgtg gtcaatcctc tcagaacgac ctccgggtac
     2281 agcgggtcgt cgggtagggg ccaggtcggt tcgatgacca ggtcgtggta ttcccacagg
     2341 gccacgatgt agggagattc agctccacct tcgggacgga agtggcagaa gatcggcatc
     2401 tctccgatca agtcgtccct gcccagatcc agcagaccgg ccttctcatc ctctgaccag
     2461 tcgatggtct gagtgtcgct ccagatggtc atcggagcgt cccgtgggat gatcccgaga
     2521 tggtctcgaa acgccacctt gagatgcagc tccgagaaga gcgggaggtc gaggccggcg
     2581 agctcggcga cctctgagct catcgggccg gcggcgatga cagtggatcc ggtcgagatg
     2641 accgacccat cgtcgagagt cacccgtcct ggtacgaggg cgtcgacgga ggctttgatc
     2701 atggtcgccc cggcatcacg agctcggtcc agcatccaca gacccaggtc ctgcgcccgg
     2761 aaccagcctg cacgtggcac gaagagggcg ccggccgcct cttctgcgat gaatggatag
     2821 gtgtcgagga gggcggtgcg gtcgagcacg tcggcgctcg ctcccattgg gtggatcgcc
     2881 cgggcctggc gctgcatctc agtcaccttc tcccgctcac cggtgacgaa caggtagccg
     2941 ttgtggttga agccgaactc ggtctcgccg gcaagctgct catagatgtc gatcgatcgg
     3001 ttcatcagcc cgaccatggc tcggtccggc caccagttcc gatagcactc cgtggatttg
     3061 tccgaggtga gggacaatgg gggacgggga tccaccagga cgacactcga gatgccctga
     3121 cccacagcca ggtgataggc cgcggagacc ccggcgatgc ctgccccgac cacgacgacg
     3181 tcggctgtgg tggtcaccgc cccaggtgtg tcttcaccct ctcggcgacc gcctccgggg
     3241 tga
//
LOCUS       DASUTA010000025         3213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_398388, whole genome shotgun sequence.
ACCESSION   DASUTA010000025 DASUTA010000000
VERSION     DASUTA010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398388"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VFZ80_00485"
     CDS             complement(<1..808)
                     /locus_tag="VFZ80_00485"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX6285935.1"
                     /translation="MPRGTGMGVSAVFACHHDMVAASDERGQSPDASRRGAGLLAHIT
                     SLPGRFGIGDLGAEAHAFVSSIAAAGQRYWQLLPVGPTGFGNSPYSSLSTFAGNPLLI
                     SPEMLCDAGLIDADELASVEMPENGEVDYGAVYLKKLGLIRMAARRFPSVAGREQQDR
                     LAVFVEERGPEWLEDYCLYDALKEAHDGRAWSEWALEVARREPGAMARARGDLQEAIA
                     INRVIQFLFFEQWYSLHRSAVEHGVSVVGDLPLYVAHDSADVWANPDLFLL"
     gene            886..1356
                     /locus_tag="VFZ80_00490"
     CDS             886..1356
                     /locus_tag="VFZ80_00490"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX6285936.1"
                     /translation="MSRIFRRGELKQAIVHVLASVGEAHGYAIMGELEARIGGGWKPS
                     PGAIYPALLGLVEAGHLEISERDGVQVYALTDSGRHLAESSEWARRWSSLNARAEESE
                     NRLAVGSLLDRFAASSAVRRRLAGPSQQARIESILARTQMEIEESLEEGEKEDG"
     gene            1349..1624
                     /locus_tag="VFZ80_00495"
     CDS             1349..1624
                     /locus_tag="VFZ80_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HEX6285937.1"
                     /translation="MDEFAELAAAEREANRIARRQWFWLHFTVFMTVQIFLFVVYQLG
                     DTGYPWFIFPLFGWGILVAAHAVYAFVVKTPEEILIERARRSNGDHR"
     gene            1621..2436
                     /locus_tag="VFZ80_00500"
     CDS             1621..2436
                     /locus_tag="VFZ80_00500"
                     /inference="COORDINATES: protein motif:HMM:NF025168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEX6285938.1"
                     /translation="MSWLLDAYTDRRTYGTLLYLLLGLPLGIVGFVVMVTGFALGIGL
                     LITLLGIPVLLATLVVVRGLADTERRLAWSLLGAPIPRIDFSILHASGSLWKRLRTLV
                     GSARTWREVAFVALRLPLGIAGFTVAMVVVGLMAGGLAQPILTAAGVETQIGDWMIDT
                     FAESLIFLPVSIVFILVGPRILLGYGTVSARMVTAVLGHIESSELKIAVARTLAHTGE
                     ADAIDITRELELMLGRGPFLTLTRVEATLIALESNGRLTATHGGNGTRYALAE"
     gene            complement(2482..>3213)
                     /locus_tag="VFZ80_00505"
     CDS             complement(2482..>3213)
                     /locus_tag="VFZ80_00505"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX6285939.1"
                     /translation="VTTRGSGTPDTGIYSAMPRSTLGERIALQILNLIRTEELRPGDQ
                     LPPERDLAKLLGVSRPVVREALRALSIMRVVEIRQGAGTFVTSLEPQQLISHLDFVFP
                     RDRVALLKLLEARRIIEVGNVRLAAERATETQLIRLEDLVARLETSIHDEQDFRALDI
                     EFHHAICEAADNFLMTQFMKIADTLGEISREETGSSPDVRKELFLSHRSVLDAVRSGD
                     PDRAESAMAEHLDRVEERLREIPAG"
BASE COUNT          552 a         1042 c         1042 g          577 t
ORIGIN      
        1 cgagcaggaa caggtcgggg ttggcccaga catcggcgga gtcgtgggct acgtagagag
       61 gcaggtcacc gacaacggac acgccgtgct ccacggcgga tcgatgcagg gaataccact
      121 gttcgaagaa gagaaattgg atgacgcgat tgatcgcgat ggcttcctgg agatcgcctc
      181 gtgcccgggc catggccccc ggctcgcgac gggcgacctc aagcgcccac tcgctccagg
      241 cccgaccgtc gtgtgcctct ttcagtgcgt cgtagaggca atagtcctcg agccattccg
      301 gcccgcgttc ctccacgaac acggcaagac gatcctgctg ctcccggccc gccacggaag
      361 ggaaacgccg cgccgccatt cggatcaaac cgagcttctt caggtagacc gccccatagt
      421 cgacctcccc gttttcgggc atctcgaccg aggcgagctc atcggcatcg atgagaccgg
      481 cgtcgcacag catctccggg ctgatcagga gcgggttgcc ggcgaatgtg gacagcgagg
      541 agtaaggcga gttgccgaag ccggtcgggc ccaccgggag cagctgccag tagcgctgac
      601 ccgccgcggc gatgctcgac acgaacgcgt gggcttcagc acccaggtcg ccgatcccga
      661 accgtcccgg caatgacgtg atgtgcgcca gcagcccggc ccctctcctg gatgcgtcgg
      721 ggctttgtcc tcgttcgtcg ctggcagcca ccatgtcatg gtggcaggcg aagaccgcgg
      781 acacacccat tcccgtgcct cttggcaaga accgggcagg ctctgagaga gcggaggaac
      841 cgtacttgac gtaacttacg tttacgcgct atcgtaagtt acgttatgag tcgaatcttc
      901 agacgaggag agctgaaaca ggccatcgtc cacgttctgg cttcagtcgg cgaggcgcat
      961 ggctacgcca tcatgggtga gctcgaagcg cgcatcggtg ggggatggaa gcccagcccg
     1021 ggagccatct accccgcgct tctcggtctc gtcgaagcgg gccatctcga gatctccgag
     1081 cgcgatggcg ttcaggtgta cgccctcacc gactcagggc gccacctcgc cgagagctcg
     1141 gaatgggctc ggagatggtc gtcgctgaat gcccgggcgg aggagtccga gaaccggctg
     1201 gccgtcgggt cgctcttgga tcgtttcgcc gccagttccg cagtgagacg gcgactggca
     1261 ggcccgagtc aacaggcacg aatcgaatcg attcttgctc gcacacagat ggagatcgag
     1321 gaatcactcg aagaaggaga gaaagaagat ggatgagttc gccgagttgg ccgcagccga
     1381 gcgagaggca aatcgaatcg cacgtcgcca gtggttctgg ctgcacttca ccgtgttcat
     1441 gacggtccag atcttcctgt tcgtcgtata ccagttgggg gacactgggt atccctggtt
     1501 catcttcccg ctgtttggct ggggcatcct ggtggcggcg cacgccgtat atgcattcgt
     1561 ggtgaagacc ccggaggaga tcctgatcga acgggcgcgc cggtcgaatg gggatcaccg
     1621 ttgagctggc ttctcgacgc ctacacggac cgccggacct acgggacgct cctatacctg
     1681 ctcctcgggt tgcccctcgg gatcgtcggg ttcgtcgtca tggtcacggg cttcgctctc
     1741 ggcatcggcc tcctgatcac cttgctgggg atcccggtgc tcctggcgac cctggtcgtc
     1801 gtcagaggtc tagccgacac cgaacgacga ctcgcctggt cgttgctggg cgctccgatc
     1861 ccgcgcatcg acttcagcat cctccacgcg agcggcagcc tgtggaagag actccgcacg
     1921 ctcgtcggct cggcgagaac atggcgggaa gtcgcattcg tcgcgcttcg tcttcccttg
     1981 ggcatcgccg gcttcaccgt ggcgatggtg gtcgtcgggc tcatggccgg cggtctggcc
     2041 caaccgatcc tcactgccgc gggggtggag acccaaatcg gcgactggat gatcgacact
     2101 ttcgctgaat cactgatctt tctcccggtc agcatcgtct tcatcctcgt cggcccccgc
     2161 atcctgctcg gctacgggac ggtgtcggct cggatggtca ctgccgttct cggtcacatc
     2221 gagagctccg agctgaagat cgcagtcgca cgcaccctgg cccacaccgg cgaggccgac
     2281 gccatcgaca tcacgaggga gctcgagctg atgctgggcc ggggcccctt tctcaccctg
     2341 accagggtcg aggcgacact catcgccctc gagtcgaacg ggcgcctgac ggcgactcac
     2401 ggtggcaacg ggacgcgcta cgccctggcc gagtgactct gacccgtctc gtgcggagcc
     2461 cgggctcctc cgacctggga cctacccggc cgggatctcc cgcagacgct cctccaccct
     2521 gtccagatgc tcggccatcg ccgattcggc tcgatctggg tcgcccgacc gcaccgcgtc
     2581 gagcaccgac ctgtgggata ggaacaactc tttgcgcacg tcgggagagg aaccggtctc
     2641 ctcacgactg atctctccca gcgtgtcggc gatcttcatg aactgggtca tgaggaagtt
     2701 gtccgccgcc tcacagatgg cgtggtggaa ctcgatgtcc agtgcgcgaa aatcctgttc
     2761 gtcgtggatc gaggtctcga gccgggccac caggtcctcg agccggatga gctgggtctc
     2821 ggtcgccctc tccgcggcca gccgaacgtt gcccacctcg atgatccggc gggcctcgag
     2881 gagcttgagg agggcgaccc ggtccctggg gaagacgaaa tcgagatggg agatgagctg
     2941 ctggggctcg agcgaggtca cgaaggtccc cgcaccctgg cggatctcca ccacccgcat
     3001 gatcgagagg gcgcgcagag cctcgcgcac cacgggccgg ctcacaccga gcagcttggc
     3061 gaggtcacgc tcggggggca gctggtcacc cgggcgaagc tcctcggtgc ggatgaggtt
     3121 caggatctgc agggcgatcc tctcccccag cgtgctccgc ggcatggcgc tgtagatccc
     3181 ggtgtcgggg gtgcccgaac cgcgggtcgt cac
//
LOCUS       DASUTA010000026         5150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1305520, whole genome shotgun sequence.
ACCESSION   DASUTA010000026 DASUTA010000000
VERSION     DASUTA010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305520"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VFZ80_00510"
     CDS             complement(<1..291)
                     /locus_tag="VFZ80_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX6285940.1"
                     /translation="MAQDYYRVLGVSRAATTEEIKKAFRRVARETHPDANPDDPTAEA
                     RFKQAAEAYEVLSDPTRRQRYDRGDTIDLSDLFSGVGGLDDLIRSVFGDSGLF"
     gene            complement(293..1288)
                     /gene="hrcA"
                     /locus_tag="VFZ80_00515"
     CDS             complement(293..1288)
                     /gene="hrcA"
                     /locus_tag="VFZ80_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX6285941.1"
                     /translation="MAMLEDRRSEVLRALVEEHIKTGEPVSSRAILERSKLAVSAATI
                     RNDLASLEHDGLVLQPHTSAGRIPTVAGYRYYVDHLGPSRLRQAAQEKVSNFFGDVHF
                     ELSKLLKATSDLLVEITDLPSVVVAPGIAREQVRAVHTVQISSDQVLVVVVTEGGRVV
                     QQRGRVRVPVTPLEIEEAQQLISAAVVGRELGSPPSFSEGYREDLSRPVEETVLTITD
                     CLHLAATGGSEMFLGGTQRMAQVWQDLSTVQRILEIMEREAEVMTLMAAAPETSIQIG
                     EDLDLAVVSKSYESGGEGGGSVGVIGPMRMNYKRVISAVEEVTRELEEQIGARSD"
     gene            complement(1292..2446)
                     /gene="hemW"
                     /locus_tag="VFZ80_00520"
     CDS             complement(1292..2446)
                     /gene="hemW"
                     /locus_tag="VFZ80_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX6285942.1"
                     /translation="MTGDETAYFDRFAEDGAAYVHIPFCSAICPYCDFAVVAGQDELA
                     GRYVAAMRAEIARSERWRPLRAIYFGGGTPSHIAPGLLASILETIDGHHGIAADAEIS
                     LEANPEDFDVHRARQLKAVGFNRVSFGAQSFDGAVLSDLGRRHGIGQIEESLNAAMAA
                     GFENLSVDLIFGTPGESDESWSRTLGLAVGTGVSHISCYALTVEKGTPLSRSVTVGAP
                     APDPDVQADRYEAANEWLGSLGLRRYEVSNWSRPGFECRYNLVVWAQGEYEAYGNGAH
                     GFRGGVRYRNHRRVDAYIERVESGESPRAGEEPVTGWDAELDRLFVGLRRTVGVRQGP
                     GTEALLASSEGRSLVDAGVLAVQNGRLSVLRPLVTDAVHRSVLGLAALSS"
     gene            complement(2443..4251)
                     /gene="lepA"
                     /locus_tag="VFZ80_00525"
     CDS             complement(2443..4251)
                     /gene="lepA"
                     /locus_tag="VFZ80_00525"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX6285943.1"
                     /translation="MTDPSRIRNFSIIAHIDHGKSTLADRLLEMTGAVTEREMREQLL
                     DSMDLERERGITIKAQTVRLEHTANDGQDYVLNLIDTPGHVDFSYEVSRSLAACEGAL
                     LLVDAAQGVQAQTLANCYLAIDAGLEVIPAINKIDLSAANPDRAAEEVIGIIGGSPED
                     VLRVSAKTGHGVAELLETLVKEVPPPTGDPSAPLRALVFDSYFDAYRGVVCYVRVVDG
                     RLDSGDDIRFMATKEVHEANEIGFLRPKAEPVASMGPGEVGYLITGVKEIDRIKVGDT
                     VTSAARPASNPLPGYDEPKPMVFSGLFPTDGDDFEKLREALEKLRLNDASLVWEPETS
                     KALGFGFRCGFLGLLHMEIVRERLEREYDLDLVATAPSVAYQVTLPDGSVRELRSPQE
                     LPEPNDRESISEPYVKAMILTPSEFVGPIMELATARRAIVGNMNYLSPERVELDFEVP
                     LAEIVFDFFDVLKSRTKGYASLDYEPAGYRESDLVKVDILLNGLPVDAFSAIVHRDNA
                     EEYGKRMTTRLRELIPRQLFDVPIQAAIGGRIISRETVKATRKDVTAKCYGGDISRKK
                     KLLQRQKEGKKRMKMVGSVEVPQEAFVAALKVDDEL"
     gene            4378..4629
                     /gene="rpsT"
                     /locus_tag="VFZ80_00530"
     CDS             4378..4629
                     /gene="rpsT"
                     /locus_tag="VFZ80_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006538885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX6285944.1"
                     /translation="MANIKSQKKRNRQNETARLRNKAIMSDLKTSMKKVQQAAAAGEP
                     TDELFRTAQKKIDTAASKGVLHPKAAARRKSRLAASINA"
     gene            complement(4809..>5150)
                     /locus_tag="VFZ80_00535"
     CDS             complement(4809..>5150)
                     /locus_tag="VFZ80_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285945.1"
                     /translation="AISKGDVGTALRRLSDFLVHGHPLVYLAALETDLKKKALSAAAP
                     DQETLRSWVGGSDRQVARLWSQRGRVRESSLRRAQDALVRADRVVKTQPEEVHRVTLE
                     RLTVAFCRWYG"
BASE COUNT          968 a         1671 c         1572 g          939 t
ORIGIN      
        1 gaagagaccg gagtcaccga acaccgagcg tatgaggtcg tcgagaccgc ccacgcccga
       61 gaacaggtcg gagaggtcga tggtgtcgcc ccggtcgtat cgttgccgcc gcgtcggatc
      121 ggacagaacc tcgtacgcct cggccgcctg cttgaagcgc gcttccgcgg tcgggtcatc
      181 gggattggca tcgggatggg tctccctggc aaccctgcgg aaggccttct tgatctcttc
      241 ggtggtcgcc gcccggctca caccgagaac tcggtagtag tcctgggcca tctcagtccg
      301 acctggcgcc gatctgctct tccagctcac gcgtgacctc ctcgacggct gagatcaccc
      361 gcttgtagtt catgcgcatc gggccgatga cgccaacgga gccgccgccc tctcccccgc
      421 tttcgtagga cttggagaca acggcgagat cgaggtcctc gccgatctgg atcgatgtct
      481 cgggagcagc cgccatcagg gtcatgactt cggcttcccg ctccatgatc tcgaggattc
      541 tctgaaccgt cgacaggtct tgccatacct gagccatccg ttgggtaccc ccgaggaaca
      601 tctcggagcc tccggtggcg gcgagatgga gacaatcggt gatggtgaga accgtctcct
      661 ccaccggtct cgacaggtcc tcgcgatagc cctcgctgaa cgaaggtgga gaccccaact
      721 cccgacccac caccgccgca gagatcagct gctgtgcctc ctcgatctcc agcggtgtca
      781 ccgggactct gacccttccc ctctgctgga cgactcgacc accttcggtg acgacgacca
      841 cgagcacctg atcgctcgag atctggacgg tgtgcactgc gcggacctgc tctcgggcga
      901 ttccgggagc gacgacgacc gaaggaagat cggtgatctc aacgagcaga tcggaggtcg
      961 ccttgagcag cttcgacagc tcgaaatgga cgtcaccgaa gaagttgctc accttctcct
     1021 gagcggcctg gcgaagacgg ctgggaccga ggtggtcgac gtagtaccgg tagccggcaa
     1081 ccgttgggat acgacctgcg gaggtatgtg gttggagaac gaggccgtcg tgctccaggg
     1141 aggccagatc attgcgaatc gtcgccgccg agaccgccag cttggaacgc tccaggatgg
     1201 cgcgggaact gaccggctca ccggtcttga tgtgctcctc gacgagggca cgcaacacct
     1261 ccgaacgacg gtcctcgagc atcgccatag gtcaagacga tagggcggcc agccccagta
     1321 ccgaacgatg cacggcgtcg gtgaccaggg gccggagaac gctcagacgc ccattctgga
     1381 cggcaaggac gcccgcatcg accagagatc gcccttccga agaggcgagc agtgcctcgg
     1441 tgccggggcc ctgtctgaca cccacggttc tgcggagccc aacgaagaga cggtcgagct
     1501 cggcgtccca acccgtgacc ggctcttccc cggcccgcgg cgattcacct gattcgactc
     1561 gttcgatgta ggcgtcgacg cgccggtggt tgcggtagcg gactccgcca cggaagccgt
     1621 gtgccccatt tccgtaggcc tcgtactcgc cctgagccca gacaacgagg ttgtaacggc
     1681 actcgaagcc aggacgactc cagttcgaga cctcgtaccg ccgcaagccg agagaaccca
     1741 gccactcatt ggcagcctcg tatctgtcgg cttgaacatc gggatcgggt gcgggggctc
     1801 ctacggtgac agagcgcgaa aggggcgtgc ccttctccac ggtcaacgcg tagcaggaga
     1861 tgtgtgacac cccggtgccc accgccagtc cgagggtacg agaccaggac tcatcgctct
     1921 cgcccggagt gccaaagatc aagtcgaccg agagattttc gaaaccagcc gccattgcgg
     1981 cattcaacga ctcctcgatc tggccgatgc cgtgtcggcg gcccagatcc gagagcacgg
     2041 caccgtcgaa gctctgagcc ccaaaagaca cccgattgaa gcccacagcc ttcagctggc
     2101 gtgcgcggtg gacgtcgaag tcctccggat tcgcctccag actgatctcc gcatctgcgg
     2161 caatgccatg gtggccatcg atggtttcga ggatggaggc gagaagacct ggggcgatgt
     2221 gactgggggt gccacctccg aagtagatcg cgcgcagtgg acgccatcgc tcggatcgcg
     2281 cgatctccgc tctcatcgcc gctacgtaac ggcctgccag ctcgtcctgg ccggcgacga
     2341 cagcaaagtc gcagtagggg caaatggcgg aacagaacgg gatgtggaca taggcggccc
     2401 cgtcctcggc gaagcgatcg aagtaggcgg tctcatcacc cgtcatagct cgtcatcgac
     2461 cttgagggcg gcgacgaacg cctcctgagg cacctcgacc gaccccacca tcttcatccg
     2521 cttcttcccc tccttctgac gctgcaggag ctttttcttt cggctgatgt caccgccgta
     2581 gcacttggcg gtcacgtcct tgcgtgtcgc cttcacggtc tcgcggctga tgatccttcc
     2641 gccgatcgcg gcctgaatgg gcacatcgaa gagttggcgg ggtatcagct ccctgagacg
     2701 ggtcgtcatc ctcttgccgt actcctcagc gttgtcgcga tgaactattg ccgagaaggc
     2761 gtcgaccggt aagccgttga ggagaatgtc gaccttgacc aggtcggact cccgatagcc
     2821 cgccggctcg taatcgaggg aggcgtatcc cttggtgcgg gacttgagga cgtcgaagaa
     2881 gtcgaacacg atctcggcca ggggcacttc gaagtcgagc tcgacacgtt cggggctcaa
     2941 gtagttcatg ttccccacga tcgcccggcg ggcagtggcc aactccatga tcggtcccac
     3001 gaactcgctc ggggtcagga tcatcgcctt cacataaggc tccgatatcg actcgcggtc
     3061 gttgggctcc ggcaactcct gaggtgagcg gagctcccgg accgaaccgt cgggcagggt
     3121 cacttgatag gcgactgagg gtgccgtggc caccagatcg aggtcatact cacgctcgag
     3181 ccgttcccgg acgatctcca tgtgcaacag ccccaggaag ccacagcgga acccaaatcc
     3241 gagagccttc gacgtctccg gttcccacac cagggaggcg tcgttcaaac gcagcttctc
     3301 cagagcctcc cgtagcttct cgaagtcgtc accatcggtg gggaacagcc ctgagaacac
     3361 catgggtttg ggctcgtcgt aacccggcaa gggattggag gcgggtcggg ccgcactggt
     3421 gacggtgtcc ccgaccttga tgcgatcgat ctccttcacg ccggtgatga ggtacccgac
     3481 ctcgccaggt cccatcgagg ccaccggctc cgccttcggt ctcaggaaac cgatctcgtt
     3541 cgcctcgtgc acttccttcg tcgccatgaa gcgaatgtcg tcccccgagt cgagtctgcc
     3601 atcgacgaca cgcacgtaac agaccactcc gcggtaagcg tcgaagtagc tgtcgaagac
     3661 cagagcgcgt agcggggcgc tcggatctcc ggtcggcggc gggacctctt tcaccaaggt
     3721 ctcgaggagc tcggcaacac cgtgaccggt cttcgccgag accctgagaa cgtcctctgg
     3781 cgacccaccg atgatgccaa tgacttcctc ggcggcccta tccgggttgg ccgcagatag
     3841 atcgatcttg ttgatggcgg gaatgacctc cagaccggca tcgatcgcca ggtagcagtt
     3901 cgccaaggtc tgcgcctgga caccctgagc agcatcgacg aggaggaggg ctccttcgca
     3961 tgccgccagg gatcgactga cttcatagga gaagtcgacg tgccctgggg tgtcgatgag
     4021 gttgaggaca tagtcctgcc cgtcattggc cgtgtgttcg agacggacgg tttgggcctt
     4081 gatcgtgatc cctcgctctc gctccaggtc catcgagtcg agcagctgct cccgcatctc
     4141 acgctcggtg acggcaccgg tcatctccag gagacgatcg gccagcgtcg atttcccatg
     4201 gtcgatgtgg gcgatgatcg agaagttgcg aatgcgcgat ggatcggtca tgtgaggtga
     4261 gtggacgtgg gcattgtacc ttgacctgct gcactgctac tcttgcccaa tccgggtctc
     4321 gacccggttc gagtcttcgg attccccact accagttgag ttttcacacg ttcagatatg
     4381 gccaacatca agtcacagaa gaagcgaaac cgtcagaacg agaccgctcg tctccgcaac
     4441 aaggcgatca tgagcgacct caagaccagc atgaagaagg tccagcaggc cgccgctgcc
     4501 ggtgagccca cggacgagct gttccgcacc gcacagaaga agatcgacac cgccgccagc
     4561 aagggcgtgc ttcaccccaa ggccgcggcc cgtcgcaagt cgcgtctcgc cgcctccatc
     4621 aacgcctaac cgcctcgccg cggctcaccg ctgggtggtc ctcggcgttg ctatacgccg
     4681 gcgatccgcc gaagcatcgt gtagcggttt ccgagacatg gtgcacggca gaccgcacct
     4741 ggctgcaccc gtggctcgac cgtccagcct gttttatcca tgattcttga taccgcgagc
     4801 ggagcgagtt atccgtacca gcggcagaag gccacggtga ggcgctccaa agtgaccctg
     4861 tggacctcct cgggctgggt cttgaccacg cggtctgccc tcaccagggc atcctgggct
     4921 cgccgcagtg acgattccct cacccgaccc cgctgcgacc acagccgtgc gacctgtcgg
     4981 tcagagcctc ccacccacga ccggagtgtc tcctgatcgg gcgcggctgc cgatagagcc
     5041 ttcttcttca ggtcggtctc caatgccgcc agatagacga gcgggtggcc gtgaaccaag
     5101 aagtcggaga gacgtcgtag agcggtgccg acatcgcctt tggagattgc
//
LOCUS       DASUTA010000027         3563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_10168, whole genome shotgun sequence.
ACCESSION   DASUTA010000027 DASUTA010000000
VERSION     DASUTA010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10168"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /locus_tag="VFZ80_00540"
     CDS             complement(<1..1539)
                     /locus_tag="VFZ80_00540"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX6285946.1"
                     /translation="MTVSPTTEQRRILDLGPTSIRVRAGAGTGKTTTVAMVIANLVEH
                     HGVDPEQILGMTFTNKAAAELAESVARRLPVAVDPSRQVEVHTYHGFAAQILAEFGPL
                     VGADTRAGVITPTFARQLIRDVFFERGHRYLDMTNRVTLDQIRKLGDQLGDHLLRPED
                     LIKAATIHGGDETWAARVEMAETLERYNRAKSDLRVVDYADLVTLSTRVLQQHPALAA
                     TVRDRYQILVLDEYQDTNPAQRVLISTIFGDGFPVIAVGDEDQTIYEWRGASAENFEQ
                     FIDHFPTADGSRAHDEGLTLNRRSGQGILDVANEIRRKANANADELEAAGDIETEIIT
                     YWASDALAEADWIARQFEALHDEGTPWSDMAVLFRKNKDFAILIDVLGRHDIPLEVAN
                     VGGLLSVPEISFLRAWLTVLHNPEDSTALAQILFGSSYRLGIADIACLTRWLTANEDD
                     DLDSEEPHPVTLVEAIEQATQIEGLRQQARERLARFHSVYRELLLETQGMSLAETCRL
                     VLDHT"
     gene            complement(1536..>3563)
                     /locus_tag="VFZ80_00545"
     CDS             complement(1536..>3563)
                     /locus_tag="VFZ80_00545"
                     /inference="COORDINATES: protein motif:HMM:NF022379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX6285947.1"
                     /translation="SSGDLPGSAGPVEPADRVGRSEIYLFDQETAEAEWIAEEVEHLI
                     RVEGARPTDIAVVVRSKRELLSELSRALGRRSVPHDPPDSRLVDHPAVRLVQDVVTAA
                     TGLVEGSPLASTAIDGALRRILLGPLVGVTVGLERQMLRERRRGTSWIDVLARHLPDQ
                     VGLRRLIAEPEWATELGAAEGFWALWTSIEGISEIVNSPERDEWRRAWTAFAQMLNRQ
                     SERDATVSLARFFELVDEDDFEATPLISHRLTEDRVSLTTLHQVKGLEFDVVFIANAV
                     EGIFPDLRRSRRMLRPELLSPERTIDPEAQHLFGVQEEMRLAYTAMTRARQRVVWTAT
                     QAGIDQGERRPSRFLLAAAGATEIGPPEETVREPVTIWEAETALRRSLLDAGRPPGER
                     LGAARVLGAPARPWWDPDSFAGAVVPGPDSPVIQGAFRLSPSQADSYRRCPRRYVLER
                     RLRLGDTTSAYAHFGELTHEILEIAEREIIGTGKRHADFARVREIIDEVWVDADFGTP
                     DLDDAWKVKAVEMLEKLYDNWPGHGEPIEVEVAVESHFEGVDWIGRVDRLERSDEGLR
                     VVDYKTGTRALTTEEAAGSIQLAFYAMAVGQTRGHVVASEMWYPRVDTKSVTTRKVAL
                     HLLDEVGEVMGEIARSISSEVWETRVSDDCKRCDFRRSCPAWPEGRGAFLA"
BASE COUNT          606 a         1156 c         1123 g          678 t
ORIGIN      
        1 ggtgtgatcg agcaccaagc gacaggtttc cgccaggctc attccctggg tttcgagcag
       61 tagctcgcga tagaccgagt ggaatcgggc gagtctctct ctcgcctgtt gtctcagacc
      121 ctcgatctgg gtggcctgtt cgatcgcctc gacgagggtg acaggatgcg gctcctcgga
      181 gtcgagatcg tcgtcttcgt ttgctgtcag ccatctcgtc aaacaggcga tgtcggcgat
      241 accgaggcga tatgagctcc cgaagagaat ctgggccagg gcggtggagt cctcagggtt
      301 gtggaggacg gtcagccacg ctcgcagaaa ggagatctcc ggcaccgaga ggagaccacc
      361 gacattggcg acctcgagcg ggatgtcgtg gcggcccaac acatcgatga gaattgcgaa
      421 gtccttgttc tttcgaaaca gcactgccat gtcggaccag ggagtccctt catcatggag
      481 tgcctcgaac tgccgggcga tccagtcggc ttcggcaagg gcgtcgctgg cccaataggt
      541 gatgatctct gtctcgatgt ctccggccgc ctcgagctcg tcggcgtttg cgtttgcttt
      601 cctccggatc tcattggcca cgtcgaggat cccctgaccc gatctccgat tcagggtcag
      661 cccctcgtcg tgagccctgc tcccatcggc ggtggggaag tggtcgatga actgctcgaa
      721 gttctccgcc gacgccccgc gccactcgta gatggtctga tcctcgtcgc caaccgcgat
      781 gaccgggaaa ccgtcgccga agatggttga gatgagcacc cgctgtgccg ggttcgtgtc
      841 ctggtactcg tccaggacca atatctggta tcgatcacgc acggtggccg ccagcgccgg
      901 gtgctgctgc aggacccgag tagacagtgt cacgagatcg gcgtagtcga cgactctgag
      961 atccgatttg gcccggttgt aacgctccaa tgtctcggcc atctcaacgc gggccgccca
     1021 ggtctcatcg ccgccatgga tcgttgccgc cttgatcagg tcctccggcc gcaagagatg
     1081 gtcgccgagc tgatcaccca gcttacgaat ctggtcgagg gtcacgcggt tggtcatgtc
     1141 cagatatcga tgacctcgtt caaagaagac gtcgcggatc agctggcggg cgaaggtggg
     1201 tgtgatgacg cccgctcggg tgtcggctcc gaccaacggc ccgaactcgg caagtatctg
     1261 tgcagcgaaa ccgtggtagg tgtgcacttc gacctgtcgt gacggatcaa ccgcgacggg
     1321 gagacgccga gcaacgctct cggcgagctc ggccgcagcc ttgttggtga aggtcatgcc
     1381 gaggatttgc tccgggtcca caccatggtg ctcgaccagg ttggcgatga ccatggccac
     1441 cgtcgtcgtc ttcccggtcc cggccccggc acggacccgg atcgaagtgg ggccgaggtc
     1501 gaggatccgc cgctgctcgg tggtggggct gaccgtcacg ctaggaatgc ccccctgccc
     1561 tcgggccaag ccgggcagga tcggcggaag tcgcatctct tgcagtcgtc gctcaccctg
     1621 gtctcccaga cctccgagga gatgctgcgg gcgatctctc ccatcacttc gccgacttcg
     1681 tcgagaaggt gcagggccac cttgcgtgtc gtgacgctct tggtatcgac tcgcgggtac
     1741 cacatctcgg aggcgacgac atgcccccgc gtttggccca ctgccatcgc gtagaaggcg
     1801 agttggatcg atccggctgc ctcttcggtc gtcaacgccc gggttccggt cttgtagtcg
     1861 accacgcgca gaccctcgtc ggagcgttcc aggcgatcga cgcggccgat ccaatcgacc
     1921 ccttcgaagt gggactcgac ggcaacctcg acttcgatcg gctcgccatg tcccggccag
     1981 ttgtcgtaca acttctccag catctcgaca gccttcacct tccaggcgtc gtcgagatcg
     2041 ggcgtcccga agtcggcgtc gacccagacc tcgtcgatga tctcgcgcac gcgagcgaag
     2101 tcggcatggc gtttacccgt gccgatgatc tctctttccg cgatctcgag gatctcgtgg
     2161 gtcaattcgc cgaagtgtgc gtatgcggag gtcgtgtctc ccagacgaag tcggcgttcg
     2221 aggacgtagc gacgcgggca gcgtcggtag gagtcggcct gactgggcga cagccggaaa
     2281 gcgccttgga tcaccggtga gtcggggccg ggcaccaccg caccggcgaa gctgtcggga
     2341 tcccaccatg gtctcgcagg ggcaccgaga accctggccg cacccagacg ctctcctgga
     2401 ggtcgcccgg catcgagaag cgaccgccgc aatgcggtct cggcctccca gatcgtcacc
     2461 ggctcccgga cggtttcctc gggcgggccg atctcggttg cgccggcggc agcaagcagg
     2521 aatcgcgacg gtcgacgctc gccttgatcg atgcccgcct gcgttgccgt ccaaacaact
     2581 cgttgcctgg ctcgtgtcat cgcggtgtag gcgaggcgca tctcttcctg gaccccgaag
     2641 agatgctgtg cctccgggtc gatcgtgcgc tccggagaca gaagctcggg tcggagcatt
     2701 cgacggctcc gccgaaggtc gggaaagatg ccctccacgg cgttggcaat gaacacgacg
     2761 tcgaactcga gccctttcac ctgatgcagc gtggtcagac tgacccgatc ctccgtgaga
     2821 cgatgagaga tcaggggcgt agcctcgaag tcgtcctcgt cgaccagctc gaagaagcgg
     2881 gcgaggctga ccgttgcatc ccgctccgat tgtctgttca acatctgggc aaaggcggtc
     2941 caggcccgtc gccactcgtc cctctccggt gagttgacga tctcggatat cccctcgatc
     3001 gaggtccaca gcgcccagaa cccctcggct gctccgagct cggttgccca ttcgggctct
     3061 gcgatcagcc ggcgcagccc tacctggtcg ggcaggtgcc ttgcgagcac atcgatccag
     3121 gaagtaccac gtcgccgttc tcgcagcatt tggcgttcga gcccgacggt gactccgacc
     3181 aaggggccca acaggatgcg ccgcagggcc ccgtcgatcg ccgtcgacgc cagtggcgat
     3241 ccttccacca ggccagtggc tgctgtgacc acgtcctgga ccagacgcac agcgggatgg
     3301 tcgacgagtc tggaatcggg tgggtcgtgg ggtactgagc gacgccccaa cgcccgcgac
     3361 agctccgaca ggagctctcg tttcgaacgg accacaactg cgatgtctgt cggccgggcc
     3421 ccctcgacgc ggatcaagtg ctccacttcc tcggcaatcc actccgcctc ggccgtttcc
     3481 tggtcgaaca aatagatctc tgagcgtccc actcgatctg cgggctcgac cggcccggcc
     3541 gagccaggca gatcgccact cga
//
LOCUS       DASUTA010000028         8026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_658298, whole genome shotgun sequence.
ACCESSION   DASUTA010000028 DASUTA010000000
VERSION     DASUTA010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8026
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658298"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1952
                     /locus_tag="VFZ80_00550"
     CDS             <1..1952
                     /locus_tag="VFZ80_00550"
                     /inference="COORDINATES: protein motif:HMM:NF006056.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6285948.1"
                     /translation="QGIISGDLVGFLTTTVGPIPIAFFVILAGASALDLWLHASGSGL
                     QVRAVGFDETAAKRGGIRTNWVRVRALILCSLFAAIAAFFIMARSPLGNAQIGSSFAL
                     NSITAAVLGGASLAGGRATFLGSAVAGVLLALIITILPFLGLTPADGMIIIGVLVVVG
                     IILFQLGDLKELVKRNFRRARRLAMGSRVQEAAAVPDFYPAANDFSVVPHSRILIKGG
                     TVLSMDPSIGDVVGGDVLIENDRIVSVGRDLDGGEGAEIIDASGMVVLPGFVDTHRHI
                     WEGVLRNIGADVPLEGRTSYISFVLHKLAPAFRPEDAYVGNLVSALGAIDAGITTLLD
                     WSHIQGSPAHTDAVVEALEDSGMRAVFAYGFPWWGKWEERQPSWFVRAATEHFSSKDQ
                     MLTLALAAPGPEFTDFEVSRDHWKLARETGARISTHVGVGSYGLDSKVQEMGEAGLLG
                     PDTTYIHCTTLNDTEIQMIVDTGGTVSLASPVEMMMGHGMPPIQKFLDRGLRPSLSVD
                     VETNVPGDMFNQMRSVLGLQRTQAAAQGRPAITTREVLACATIDGAKANGLDHKTGSL
                     TPGKQADVVLLRTDRMNVTPLNDPVTAVVAGMDTGNVDTVIIAGRVMKRRGQLEHVDW
                     PAVRRAVEESRDYVIEKSGFKLPKI"
     gene            2156..3253
                     /gene="proB"
                     /locus_tag="VFZ80_00555"
     CDS             2156..3253
                     /gene="proB"
                     /locus_tag="VFZ80_00555"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX6285949.1"
                     /translation="MRRPVRSGSPIVVKIGTSSLTSAAIGIDPEAIERVVVNIESSWE
                     QGHPTVLVTSGAIAAGLPAMGLSERPRDIPGLQVAAALGQGRLMTDYTAEFARHGRRV
                     GQILLTRDVLARREQYLNARAAMERMLSLGVVPVVNENDTVVVDELRFGDNDRLAAIV
                     SHLVGAGMLVMLTDTPGIYSDDPWATESAELLTVVRHTDSILDQLRTGSNRGSFGSGG
                     VATKVAAARMAAWSGIPTVIASASEPDVVTRAVSGDDVGTWVEPRESSLSARKLWIAF
                     GSPPEGKVVVDAGAAHALSAGGKSLLAVGVTEVEGTFPVGVAVEVHHSGDLVAKGLTS
                     MSAADIAAMRGQHSDVAGGEVIHRDDLVVLT"
     gene            3261..4760
                     /locus_tag="VFZ80_00560"
     CDS             3261..4760
                     /locus_tag="VFZ80_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6285950.1"
                     /translation="MSEHLTRLEEGMPIVYGGNRVTRVSAALADAFAEGDRLVVVQDS
                     GDLLHIPRADWERASQAVAAAAEAFSDIGRLTTDQLDAFFLAFASYLEDDTSFAPVAE
                     ANASDVERAVERGRSTTRLALTDRMRADMAEGLRLWVGGDLGRGEVLETIDHDGWALE
                     QVRSGLGVVGFVFEGRPNVFADATGVLAGGNTVVFRIGSDALETARAIMENALNPALK
                     DAGLPDGAVSLVDAPSHAAGWAMFSDPRLGLAVARGSGAAVSQLGSVARQTGIPVSLH
                     GTGGAWIVAAATADPDAFSAAVYNSLDRKVCNTLNTACIVASRAADLVPVFLDALDRS
                     GKRRNASAKLHMTHAAREYVPDEWFEPATIVRAGGPAEETRSEIIGDDELGIEWEWEE
                     SPEVSLHIAPDVETAIRLFNEQSPRFVASLVSEDLEEHRVFFDTIDAPFVGNGFTRWV
                     DGQYALHRPELGLSNWQFGRLFGRGGVLSGDSIFTVRTRVWQSDPDVGR"
     gene            4794..5351
                     /locus_tag="VFZ80_00565"
     CDS             4794..5351
                     /locus_tag="VFZ80_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285951.1"
                     /translation="MIDTVPGDELPTDDEWSDWEGGFEPIEAAPTWRKPLLIAVAAVT
                     AIALAMLPLYNVIRARTVAENGLEVCGFDYCVVQEAAVDAGVDLVMSRLWNTFLDEES
                     AREFAARLTDYLGIDPVGLQIVGDLEGRLGGVYDPETRSIQVAGPARAWTVLHEVAHA
                     VETGHGPEFRELVVDLARWVDSGEG"
     gene            5396..5836
                     /locus_tag="VFZ80_00570"
     CDS             5396..5836
                     /locus_tag="VFZ80_00570"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEX6285952.1"
                     /translation="MRDWLLFFHILGAAGWIGGGLYGWFTTTQIVKSGSTSGRALAML
                     AGTADRYFGPVAVLTLLTGIALVWTQDPWGWNDAFVLIGIGVFVFSAIYQPLVSSKSE
                     KQLLDAIESGSDTSAAMRTTHRVLAVDLAVVLVALWAMVVKLGA"
     gene            complement(5846..6631)
                     /locus_tag="VFZ80_00575"
     CDS             complement(5846..6631)
                     /locus_tag="VFZ80_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEX6285953.1"
                     /translation="MGDLLLVAALAIAVLMLVTWIVSVIVNDASIVDIVWGLGFAIAT
                     AVAFFSSPNDTMTDRSLLMLVMVGVWGLRLSAYLAWRNIGKGEDRRYQQMRKKSPDSF
                     WLVSLYKVFGLQAILMWIVAVPAVVVHAIDEPLYWLDFVGLGIWAVGLFFEAVGDIQL
                     ARFKARPDSKGKVMDAGLWRYTRHPNYFGDFCVWWGIYLVAAAGGAWWAIFSPLVMSA
                     LLMRYSGAGLLEKTIGRRRPEYEEYAKRTNAFFPGPPRQPAGS"
     gene            complement(6699..7157)
                     /locus_tag="VFZ80_00580"
     CDS             complement(6699..7157)
                     /locus_tag="VFZ80_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX6285954.1"
                     /translation="MKETTPDRIRAWRGVMHVHARVTEEIDRTLRSEVGIPGAWYEAL
                     VEIAFAGGHMRMNEFACETTLTRSGATRFVDRLETAGLVERTVCPTDRRVQQLALTAE
                     GKRVQQAADPHVLKVIDEHFGRFLDDDEAATMSDALQRMRRDLVPADQGR"
     gene            7238..7792
                     /locus_tag="VFZ80_00585"
     CDS             7238..7792
                     /locus_tag="VFZ80_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010649664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX6285955.1"
                     /translation="MTTETTTRPDVETGVWTIDKNHTKVGFVAKHLMVTKVRGHFESY
                     DATVEIADDLTDSHIEATFDSASLTTGAADRDGHLRSNDFFDVENHPTMKFVSTDIAR
                     DGDDWTITGDLTIRDTTHPVTLDAVYEGSAVDPWGNTHIGFSAKATINREDWGLTWNA
                     ALEGGGWLVSKEVVIEIEGQLVRP"
BASE COUNT         1420 a         2571 c         2585 g         1450 t
ORIGIN      
        1 ctcaaggaat catcagcggc gacctcgtcg gctttctcac cacgacggtc ggcccgattc
       61 cgatcgcttt cttcgtgatc ctggccgggg cgagcgccct cgatttgtgg cttcacgcgt
      121 ccgggtctgg ccttcaggtg cgggctgtcg ggttcgacga aaccgcggcg aaacggggcg
      181 ggatcagaac caactgggta cgggtacgcg ccttgatcct ctgcagtctg ttcgccgcca
      241 tcgccgcatt cttcatcatg gcccgctcgc ctctcggcaa cgctcagatc ggatcgtcgt
      301 tcgccctgaa cagcatcacg gctgcggtcc tgggaggggc gtcgcttgcc gggggccggg
      361 caacattcct gggcagcgcg gtagccggcg tcctcctcgc cctcatcatc accatcctgc
      421 ctttcctcgg gctgacaccg gctgacggaa tgatcatcat cggtgtcctc gtcgtcgtcg
      481 ggatcatcct cttccagctg ggtgacctca aggagctggt gaagcggaat ttccggcgag
      541 cccggcgtct ggccatgggc agccgggtgc aggaggcggc ggcggtcccg gatttctatc
      601 cggctgccaa cgacttcagt gtggtgccgc acagtcgcat cctgatcaag ggcggaaccg
      661 tcctctcgat ggacccctcg atcggagatg tggttggcgg cgatgtcctc atcgagaacg
      721 accgaatcgt ctccgtgggc cgggacctcg acgggggcga gggcgcagag atcatcgatg
      781 cctcaggaat ggtggtgctc cctggcttcg tggacaccca ccgacacata tgggaagggg
      841 tgctgcgcaa catcggtgcc gacgtgcctc tcgagggccg caccagctac atctcgttcg
      901 tgctacacaa gctggcaccg gcgttccgcc ccgaagacgc ctacgtgggc aatctggtct
      961 cggcgctcgg cgccatcgac gccgggatca ccacccttct cgactggtcc catatacaag
     1021 gctccccggc ccataccgac gccgtggtcg aggctctcga ggactccggc atgcgggcgg
     1081 tgttcgcata cggctttccg tggtggggga agtgggagga gcgccagccg agttggttcg
     1141 tccgcgccgc cacggagcac ttctcctcga aggaccagat gctgacactc gctctggctg
     1201 cccccgggcc cgagttcacc gacttcgagg tctcacgaga ccactggaag ctggcacgag
     1261 agaccggtgc ccgcatctcg acacatgtcg gtgtgggcag ctacgggctc gattcgaagg
     1321 tccaggagat gggggaggcc ggtctgctcg gtcccgacac cacctacatc cactgcacga
     1381 ccctcaacga caccgagatc cagatgatcg tggacaccgg aggcaccgtc tcactcgcct
     1441 cacccgtcga gatgatgatg ggccacggca tgcccccgat ccagaagttc ctcgatcggg
     1501 gtctccgccc cagcctcagc gtcgacgtcg agaccaacgt gccgggcgac atgttcaacc
     1561 agatgcgctc ggtgctgggg ctgcaacgca cccaggccgc cgcccagggc agacccgcca
     1621 tcaccactcg agaagtcctg gcctgcgcca cgatcgacgg cgccaaggcg aacgggctcg
     1681 accacaagac cggcagcctc acccccggca agcaggccga cgtcgtgttg ctccgcaccg
     1741 atcggatgaa cgtcaccccg ctcaacgacc cggttaccgc ggtggtggcc gggatggaca
     1801 ccggcaacgt cgacacggtc atcatcgccg ggcgtgtcat gaagcgccgt ggtcagctcg
     1861 agcatgtcga ctggccggcg gtgagacgcg ccgtcgagga gtcgcgcgac tacgtgatcg
     1921 agaagtcggg cttcaagctt cccaagatct gaccagaatc cacgaacccg gggttcctgc
     1981 gtttcctttg gacacgttgg agtttccaag gaactccttt cctgtcgaaa gtcgttcgac
     2041 aggcccggat acgacccaaa tcagctcatc ttcggtgttc tgactgaacc gggcggtgag
     2101 cgcagctaaa ccacgaaggt cgtaaacagc ttcgccgcta ccgtggccgg ttccgatgag
     2161 acgacccgtc cgctcaggct caccgatcgt tgtgaagatc ggcacctcca gcctcacctc
     2221 ggccgcgatc gggatcgatc cggaagcgat cgagcgggtg gtggtcaaca tcgagtcgag
     2281 ctgggagcag ggccatccca cggtgctcgt cacctcgggg gccatcgccg ccgggttgcc
     2341 ggccatgggc ctgtccgagc gaccgcgcga cattcctggt ctccaggtgg ccgcggcatt
     2401 gggtcaaggc cggctcatga ccgactacac cgcggagttc gccaggcacg ggcgacgcgt
     2461 tggccagatc ctcctcaccc gtgacgtgct cgctcggcgg gagcagtatc tcaacgcacg
     2521 cgccgccatg gagcggatgc tctcgctcgg ggtcgtgccc gtcgtcaacg agaacgacac
     2581 cgtggtcgtc gacgagctgc gcttcggtga caacgaccgg ctcgccgcca tcgtctctca
     2641 cctggtgggg gcggggatgc tcgtcatgct caccgacact ccaggcatct actccgacga
     2701 cccgtgggcc accgagtcgg cggagctcct cacggttgtg aggcataccg attcgatcct
     2761 cgaccagctg aggacaggct ccaatcgggg ctcgtttgga tcgggaggag tggcaaccaa
     2821 ggtcgccgcg gctcgcatgg ccgcctggtc ggggatcccg acggtcatcg cgtcggcctc
     2881 cgagccagat gtggtcaccc gagcggtgtc cggtgacgac gtgggaacct gggtggagcc
     2941 cagagagtcg agtctgtcgg cgcggaaact ctggatcgcc ttcggctcac ctccggaggg
     3001 aaaggtggtc gtcgatgccg gtgccgctca cgctttgagc gctgggggca aatcgctcct
     3061 ggccgtgggc gtcaccgagg tcgaggggac gttccccgtc ggtgtcgccg tcgaggttca
     3121 tcacagcggg gatctcgtcg ccaaaggcct cacctcgatg tcggctgccg acatcgcagc
     3181 aatgagagga cagcactccg atgtggccgg aggcgaggtc atccaccggg acgatctcgt
     3241 cgtgctgaca taatcctggg atgtcggaac acctgacccg actcgaagag ggaatgccca
     3301 tcgtctatgg cggcaaccgg gtgacccggg tcagcgccgc cctcgctgat gccttcgccg
     3361 agggagaccg cctcgttgtg gttcaagaca gcggagacct gctccacatc ccccgggccg
     3421 actgggagcg cgcctcccag gccgtcgctg ccgccgccga ggcgttctcc gacataggga
     3481 gactcaccac cgatcagctc gatgccttct tcctcgcctt cgcctcctat ctcgaggatg
     3541 acacgagttt cgccccagtg gccgaggcca atgcatcaga tgtggaacgc gctgtcgaaa
     3601 gaggcaggtc gacgacgaga ctggcgttga ccgaccggat gcgagcagac atggcggagg
     3661 ggctccgcct gtgggtcggc ggtgacctcg ggcgagggga agtgctggaa acgatcgacc
     3721 acgatgggtg ggccctcgag caagtcagat ccggtctcgg cgtcgtcggc ttcgttttcg
     3781 aagggcggcc caacgtcttc gcagatgcca caggcgtcct cgccggcggc aacaccgtcg
     3841 tcttccgcat tggatccgat gcattggaga cggcccgggc catcatggag aacgccctca
     3901 accctgcctt gaaggatgcc gggctcccag atggtgcggt atcactcgtc gacgcgccgt
     3961 cacacgcagc gggttgggca atgttctccg accccagact cgggctcgcg gtggcgcggg
     4021 gatcgggcgc cgcggtgagt cagctcggct cggtggccag acaaaccggt atcccggtga
     4081 gtctgcacgg caccggtggc gcgtggatcg tggcggcggc gaccgctgat cccgatgcgt
     4141 tctccgccgc cgtttacaac tcgctcgata ggaaggtgtg caacaccttg aacacggcct
     4201 gcatcgtggc ctcgcgagct gccgacctcg tgcctgtctt cctcgacgcc ctcgatcggt
     4261 ccggcaagcg acgcaacgca tcggcaaagc tccatatgac ccacgcggcg cgggaatacg
     4321 tgcccgatga atggttcgag ccggcgacga tcgtgcgggc cggagggccg gccgaggaga
     4381 cccgatcgga aatcatcggc gatgacgagc tcggcatcga atgggagtgg gaggagagcc
     4441 ccgaagtcag tcttcacatc gccccggatg tcgaaacggc gatccgactc ttcaacgagc
     4501 agagtcctcg cttcgtcgcc agtctcgttt ccgaagacct cgaagaacat cgcgtgttct
     4561 tcgacacgat cgatgctccc ttcgttggga acgggttcac ccgctgggtc gacggccaat
     4621 atgctctgca ccggcccgag ctcggcctgt ccaactggca gttcggtcgt ctcttcggcc
     4681 gcgggggagt cctttcgggc gactcgatct tcacggtgag aacccgtgtc tggcagtccg
     4741 accccgacgt cggtcgttga ccgattgcac ggatcggccc atagcatcga attgtgatcg
     4801 ataccgtgcc gggcgacgag ctccccaccg acgacgagtg gagcgactgg gagggagggt
     4861 ttgagccgat cgaggccgcg cccacctggc gtaagcctct gctcatcgcg gtcgccgcgg
     4921 tgactgcgat cgctctcgcc atgctccctc tctacaacgt gatccgggcg aggacagtgg
     4981 ccgagaacgg gctcgaggtg tgtggcttcg actactgcgt cgtccaggag gctgcggtgg
     5041 acgccggtgt cgatctcgtc atgagccggc tttggaacac ctttctcgac gaggagtcgg
     5101 cacgggagtt tgccgcccgg ctcaccgact acctcgggat cgacccggtt ggcctgcaga
     5161 tcgtcggcga tctcgaggga agactcggtg gggtctacga ccctgagacc cgttcgattc
     5221 aggtcgcggg accggcccgc gcctggaccg tgctccacga agttgcccat gccgtcgaga
     5281 ccggccatgg ccccgagttc cgcgagctgg tggtcgatct ggccagatgg gtcgattccg
     5341 gcgagggatg aaactccgcc aggggagggc ccgacgcgat aacttgtcgg gcgtcatgcg
     5401 cgactggctc ctcttctttc acattctcgg cgctgccggc tggatcggtg gtggcctgta
     5461 cggctggttc acaactactc agatcgtcaa gagtggatcg acttcaggga gggcgttggc
     5521 aatgttggcc ggcaccgccg accgatactt cgggccagtt gccgtgctga cgcttctcac
     5581 cgggatcgcc ctggtgtgga cacaggatcc ctggggatgg aacgatgcct ttgtgctgat
     5641 cggcatcggc gttttcgtct tctctgccat ctaccagcct ctggtgtcat cgaaatccga
     5701 gaagcagttg ctcgatgcga tcgaatccgg gagcgatacg tcggcggcca tgaggacgac
     5761 tcatcgggtg ctggccgtcg acttggccgt cgtcctcgtt gccctctggg cgatggtggt
     5821 caagttggga gcttgaaagc ggggatcagg atcccgctgg ttgccggggc gggccgggga
     5881 agaaggcatt ggtcctcttg gcgtactcct cgtactcggg tcggcgccga ccgatcgtct
     5941 tctccagcaa gccggcgccc gagtaacgca tgaggagggc ggacatgacc agaggtgaga
     6001 agatggccca ccatgcccct ccggcggcag ccaccaggta gatgccccac cagacgcaga
     6061 agtcaccaaa gtagttgggg tgtctggtgt atcgccagag ccctgcgtcc atgaccttgc
     6121 ccttggagtc gggtcgagct ttgaacctcg ccagctggat gtcgccgacc gcctcgaaga
     6181 agagaccgac tgcccagatc cccagaccga cgaagtcgag ccagtagagc ggctcgtcga
     6241 tggcgtggac gacgacggcc ggcacggcca cgatccacat cagtatcgcc tgaagcccga
     6301 agaccttgta cagactgacc agccagaacg agtcggggct cttctttcgc atctgctggt
     6361 agcggcggtc ttcacctttg ccgatgttgc gccaggcgag ataggcggac aggcgaagac
     6421 cccagactcc gaccatgacg agcatgagga gggaccgatc ggtcatggtg tcgttcgggg
     6481 aggagaaaaa ggcgacagcg gtggcgatcg cgaagccgag accccagacg atgtccacga
     6541 tggatgcgtc gttgacgatg acgctgacga tccaggtcac cagcatcagc accgcgattg
     6601 ccagagcggc aactaggagc aggtcaccca tgtggatcgt ttctcagagc ggggagagac
     6661 taggccatcg aatgggttgt agcccgatgt agacgggctc atcgcccctg gtcggcaggg
     6721 acgaggtccc gacgcatcct ctgcagcgca tcggacatgg tcgcagcttc gtcgtcgtcg
     6781 aggaacctgc cgaagtgctc atcgatgacc ttcaagacgt gcgggtcggc tgcctgctgt
     6841 acccgcttgc cctcggctgt gagggcgagt tgctgcaccc ggcgatctgt cgggcatacg
     6901 gtcctctcca cgaggccggc ggtctcgaga cgatccacga agcgggtggc gccgctcctg
     6961 gtgagggtgg tctcacaggc gaactcgttc atccgcatgt gaccaccggc gaaggcgatc
     7021 tcgaccaacg cctcgtacca agccccaggt atccccacct cgcttctgag agttcggtcg
     7081 atctcctcgg tgacccgggc gtggacatgc atcacgccgc gccacgctct aatccggtct
     7141 ggcgtcgtct ctttcacctt tatcagcata gatggaataa ttgcctgact caatggttgc
     7201 agtaatcaac taatcacgga tacgcaaagg agagaccgtg accacagaga caacgacacg
     7261 cccggacgtc gagaccggcg tgtggacgat cgacaagaac cacaccaagg tcggcttcgt
     7321 cgccaagcac ctcatggtga ccaaggtgcg aggccatttc gagtcatacg acgccaccgt
     7381 cgagatcgca gacgatctga ccgactcgca catcgaggcg accttcgatt cggcaagcct
     7441 caccacggga gcggccgatc gtgacgggca tctccgctcg aacgacttct tcgacgtgga
     7501 gaaccacccg acgatgaagt tcgtttcgac cgacatcgcc cgcgatggcg acgattggac
     7561 gatcaccggc gatctgacca tccgggacac cacccacccg gtcaccctcg acgccgtgta
     7621 tgaaggcagc gccgttgatc cgtggggaaa tacccacatt ggcttcagtg ccaaggccac
     7681 gatcaaccgt gaggattggg gtctgacctg gaacgccgcc ctcgaaggcg gcggctggct
     7741 ggtctcgaag gaggttgtca tcgagatcga gggccagctc gtccgtccct gaatcagacg
     7801 acaatctggc cccggcatct ccaggccggg gccagatgct cttcggcgcc gcatctactg
     7861 ggcctaatcg acggccttgc ttaccatttg ccctcctgtg ccacgtccca attggacaga
     7921 gtgcgaggac ggctggacga gcggccccta cctcatcgag accctgggcg gcagtctgtg
     7981 ggtgctatcc cgccgttgcg gatctggcga gcggctgctg acctcc
//
LOCUS       DASUTA010000029         2937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_269681, whole genome shotgun sequence.
ACCESSION   DASUTA010000029 DASUTA010000000
VERSION     DASUTA010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269681"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VFZ80_00590"
     CDS             <1..399
                     /locus_tag="VFZ80_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012855119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX6285956.1"
                     /translation="AADLGIDYSLSNTLEVVDGFLTGDLAGELVDGPRKGTFLQEIAA
                     REGISPAQVVAVGDGANDIDMLSVAGLGIAFNAKPVVKERADTSVSVPYLDAILFLMG
                     VRRDHVEAADAADPEFEQNGLIEIPGTPPA"
     gene            complement(486..1514)
                     /locus_tag="VFZ80_00595"
     CDS             complement(486..1514)
                     /locus_tag="VFZ80_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6285957.1"
                     /translation="MGYTITEVSIDDLSEGAFEHVVTVANALDAEAFPRHVDNTAEEL
                     RIFMNSPGLTHHRFLVHGDGQLVALASFRYPDDGTNPDTLSCQIRVVPAHRRQGIATS
                     LLARAVDLASDIGRKTLQGFIFDTVPASRAFIEAIGARATVDFHENVVRIADLDVELL
                     QSWVDGGPQRAPGYSVELIEGSWPEKHFDDIAHLFHILERDMPTSDAFEPREWNAQRI
                     SELQDHYAKGVDSLSTLAIHDQTGRVVGMSDMIRRKVDPTTWIVTTTVVDPEHRGRAL
                     GKWVKGAINLAALERWDGGVYQETGNAFVNEPMLAINRAMGFEHELTMTDCLLSIEDA
                     RTYLGTRG"
     gene            complement(1514..2920)
                     /gene="gnd"
                     /locus_tag="VFZ80_00600"
     CDS             complement(1514..2920)
                     /gene="gnd"
                     /locus_tag="VFZ80_00600"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEX6285958.1"
                     /translation="MTQRSDIGVVGLGVMGANLALNMADHGNRVTVYNRTASVTDEFM
                     TGEAGEKEIVPTTTLQELVESLNAPRIVLVMVKAGAPVDYVIGDLAPLLDRGDIVIDG
                     GNSLFSDTERRHRELQERGIRFIGAGVSGGEEGARHGPSIMPGGDPSAWPEVKDILQG
                     IAAKAPDGSPCCEWMGPGGAGHFVKMVHNGIEYGDMQVIAEAYDLMARGLGLEPAEIG
                     DVFSRWNRGRLQSYLVEITAEILSTHVDGSPLIDRILDAAGQKGTGKWTVIASMEAGE
                     PTTLVAEAVYARIVSSDPDRRAVASELFPSPIEVVTDIGADEIESALYASKIVSYAQG
                     FRLLQAASDEHGWDLDMGTVASIWRAGCIIRAVFLEDITAAYRANANLSDLMEADFFS
                     SALRHEEAAWRRVVGAAVSSGIPTPAYSSALAYFDGIRSRRLPASLIQAQRYYFGAHT
                     FERTDRPRGEFFHHEWNE"
BASE COUNT          522 a          915 c          918 g          582 t
ORIGIN      
        1 gccgccgacc tgggcatcga ctactcgctg tccaacacgc tcgaggtagt cgacggcttc
       61 ctgaccggtg atctggccgg ggagctcgtc gatgggcctc gaaaaggaac ctttctccaa
      121 gagatcgctg caagagaggg catctcgccg gctcaggtcg tcgccgtggg tgacggtgcc
      181 aacgacatcg acatgctctc ggtggcgggc ctcggcatcg ctttcaacgc caagcctgtg
      241 gtcaaggagc gagccgacac ctcggtgtct gttccttatc tcgacgccat cctcttcctg
      301 atgggcgtgc gccgtgatca tgtcgaggcc gccgatgccg ccgatcccga gttcgagcag
      361 aacggcctga tcgagatccc cgggacgccc ccggcctgac ttggccaccg tcttgattcc
      421 tccaactgac gtgtagcgaa caaggcatag gcgtgttcgt cacacgtcag acgcgtgggc
      481 ggcggtcagc ctctggtacc tagataggtc ctggcgtcct cgatgctcaa caggcaatcg
      541 gtcatggtca actcatgctc gaagcccatg gcccgattga tggcgagcat cggctcgttg
      601 acgaaagcgt tgccggtctc ctggtagacc ccgccgtccc agcgttccag cgcggcgaga
      661 ttgatcgctc ccttgaccca tttccccaac gcccggcctc ggtgctcagg gtccaccact
      721 gtcgtggtca cgatccaggt cgttggatcg acttttctcc tgatcatgtc gctcataccc
      781 acgactcgac cggtctggtc gtggatcgcc agtgtcgaga gcgagtccac ccctttggcg
      841 tagtgatctt gaagctcaga gatccgctgg gcgttccact ctctcggctc gaaggcgtcg
      901 gaggtaggca tgtcacgttc gaggatgtgg aacagatgtg cgatgtcgtc gaagtgcttc
      961 tccggccacg agccttcgat gagctccacc gagtagccgg gagcccgctg cgggccgcca
     1021 tctacccagc tctgcagtag ctcgacgtcg aggtctgcga tgcgaacgac gttctcgtgg
     1081 aagtcgactg tcgccctggc accgatggcc tctatgaatg cgcgggaggc gggcaccgtg
     1141 tcgaagatga agccttgaag ggtcttgcgt ccgatatccg acgcaagatc gaccgcccgg
     1201 gcgagcagcg aggtagcgat gccctgtcgc ctatgtgcgg gaaccacccg aatctgacac
     1261 gagagggtgt cggggttggt cccgtcgtcg ggataccgga acgaggccag ggcaaccagt
     1321 tgtccgtccc cgtgcaccag gaagcgatga tgggtcaatc ctgggctgtt catgaaaatg
     1381 cgaagttcct cggctgtgtt gtccacatgt cgcgggaagg cctccgcgtc cagagcattg
     1441 gcgaccgtca ccacgtgttc gaatgcgcct tcggagaggt cgtcgatgct gacctcggtg
     1501 atcgtgtagc ccatcactca ttccactcgt ggtgaaagaa ctcgcctctc ggtcggtcgg
     1561 tgcgctcgaa tgtgtgcgca ccgaagtagt aacgctgtgc ctgtatcaag ctcgcaggca
     1621 accgccggga tcgtatgccg tcgaagtaag cgagtgcgct ggaataagcg ggagtgggga
     1681 tgcccgagga gactgcagcg ccaacgaccc gcctccacgc agcctcctcg tggcgtaggg
     1741 ccgagctgaa gaagtctgcc tccatcagat cgctcagatt ggcgttcgcc ctgtatgccg
     1801 cggtgatgtc ctcgagaaag acggcgcgaa tgatgcaccc ggccctccag atcgaggcca
     1861 cggtacccat gtcgagatcc cacccatgct cgtcagaggc agcctggagg agccggaatc
     1921 cctgtgcata ggagacgatc ttcgaggcgt acagagcaga ttcgatctcg tcggccccga
     1981 tgtcggtcac gacctcgatg ggagagggga acaactccga tgcgacggcc cgtcggtccg
     2041 ggtcggaact cacgatgcgg gcgtacacgg cttcggcgac gagggtggtt ggctcgcccg
     2101 cttccattga ggcgatgacg gtccactttc cagtaccttt ttgtccggcc gcgtcgagga
     2161 tgcggtcgat gagcggcgac ccgtccacgt gggtgctgag gatctcggct gtgatctcaa
     2221 cgagatacga ctggagacgt ccgcggttcc atcgggagaa gacgtctcca atctcggccg
     2281 gttccaggcc taggccgcgg gccatgaggt cgtaggcctc ggcgatcacc tgcatgtccc
     2341 cgtactcgat gccgttgtgg accatcttca cgaagtgtcc ggctccgccc gggcccatcc
     2401 actcacaaca cggcgaaccg tccggggcct tcgctgcgat gccctggagg atgtctttga
     2461 cttcgggcca cgcggaagga tctcccccgg gcatgatcga gggcccatgt ctggcgcctt
     2521 cctcaccacc ggagaccccg gctccgatga agcggatgcc tctctcctga agctcccgat
     2581 ggcggcgctc tgtgtccgag aacagggagt ttcccccatc gatgacgatg tctcccctgt
     2641 cgaggagagg agcgaggtcg cctatcacgt aatcgaccgg cgccccggcc ttgaccatga
     2701 cgagcacgat cctcggggca ttcagcgact ccacgagctc ttgcaaggtc gtggtcggaa
     2761 cgatttcctt ctccccggcc tcgcctgtca tgaattcgtc ggtgaccgaa gcagtccggt
     2821 tgtagacggt cactcgattt ccgtggtcgg ccatgttgag ggccagattg gcgcccatca
     2881 ccccgaggcc gacgacgccg atgtcagatc gttgggtcac atgaagaggc tattacg
//
LOCUS       DASUTA010000030         2944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_10989, whole genome shotgun sequence.
ACCESSION   DASUTA010000030 DASUTA010000000
VERSION     DASUTA010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10989"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1173)
                     /locus_tag="VFZ80_00605"
     CDS             complement(130..1173)
                     /locus_tag="VFZ80_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6285959.1"
                     /translation="MTNKSDGESAVQAASQPRQGQATAGRSEPGLAERYALPVLFLIL
                     IVGFSLALPDTFASVANLRAILTTQAVPAIAAMALMLPLVAGRFDISIGAIIGVCSIV
                     TAAAMSTYNLSLPVAIGLALIVGLVVGAANGLLVAYLGVNSIIATLGTTIILSGLIQA
                     YTRGIPISSGLSPTLTSLSAARWLGVPALFVIMLVVAILVSLLVNQTPFGRYMTATGS
                     NEIAAQLNGMAVRRILSGSFAVAGLLAGMAGVLQVAAQGNGNPQVGGIGFILPALAAV
                     FLGATTLRPGTYNIPGTILALYFIAVTVIGLALLGAQPWVTDVVNGISVVVAVTLSAQ
                     FRRRRTGESALGS"
     gene            complement(1179..2747)
                     /locus_tag="VFZ80_00610"
     CDS             complement(1179..2747)
                     /locus_tag="VFZ80_00610"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020124616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX6285960.1"
                     /translation="MTSSTGDMPPGSNRGDISLEVRRLSKTYAGGPALRDVSFGLERA
                     KVHALLGGNGSGKSTLIKCLAGVTPADSGGEIVVGGESIDADKVTPAWSRHHGLRFVH
                     QHSTTFGTMTVEENLALGHGYPTVVGFVRWRALRTKTKDLLDRFGITAKPDQPMSSVR
                     PADQAMIAVARALQDRYETEASAISTLVLDEPTAAMPEEEVDVLLEAIKSCARDGVAI
                     VFVTHRLDEVRAIADTVTVLRDGLLAASQPAGELTQSDLVELILGEEFGELARQKRTR
                     TPGSVVARLRSVTAGPLRDVSLDVREGEVLGIAGLTGSGRSELLRTIFGDMRTDGGQV
                     EVLGTNLENATPGAAIARGVAYVPEERSAAVFPSLSVRENLSAPSVSRYARGPLLDRA
                     KERSDSDDVVEKYSIKAPSSEAAISTLSGGNQQKVVLARWLERKPAILLLDEPTQGVD
                     VGARADVFALIREAANDGLAVVIVSSDFEELADMSDRVLVLNNGRLVTELTGKDINRR
                     AINEEVLMGSGVSS"
     gene            complement(2754..>2944)
                     /locus_tag="VFZ80_00615"
     CDS             complement(2754..>2944)
                     /locus_tag="VFZ80_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX6285961.1"
                     /translation="EWIFWGLADSLNRIFAGEDPATLPNQGGGWQFVDAEHNIPPEGE
                     SYSPPVDFRTAYTAIWEG"
BASE COUNT          535 a          937 c          929 g          543 t
ORIGIN      
        1 ccgtcgatga ggacatccgc ctttgcgaag tcacccagct ccttgtcgag agtgaggacc
       61 gttcctccct ttatgagctt cttcccgctc attcgttctc cttccattct ccgggttcga
      121 tgtgcaagat caggagccca acgccgattc accggtgcga cgccggcgga actgtgcgga
      181 gagcgtgacc gcgacgacaa cggagatgcc gttgaccacg tcggtcaccc aaggctgggc
      241 cccgagcaag gcgaggccga tgaccgtcac cgcgatgaag tagagagcga ggatggtgcc
      301 cgggatgttg tacgttcccg gccggagtgt cgtggcgccg agaaacacgg ctgccagcgc
      361 agggaggatg aagccgatcc ctccgacctg tgggttgccg ttcccttggg cggccacctg
      421 cagcacacct gccatgcccg ccaggagtcc ggcgacggca aagctcccgg acaagatccg
      481 ccgtaccgcc atgccattca gctgcgcggc gatctcattc gaccctgtag ccgtcatgta
      541 ccgaccgaac ggtgtctgat tgaccaggag actgaccagg atcgctacta ccaacatgat
      601 gacgaacaga gcgggaactc cgagccatct cgccgcactg aggctggtga gggttggcga
      661 caatcccgag ctgatcggga tcccgcgggt gtaagcctgg atcaagcccg agaggatgat
      721 cgtcgtgccc aatgtcgcaa tgatcgagtt gacgccgaga taggccacga gaagcccgtt
      781 ggcggcgcca acgaccaggc caacgatgag tgccaggccg atcgcgaccg gtagtgacag
      841 gttgtacgtc gacatggcgg ccgcggtcac gatggagcac acaccgatga tcgccccgat
      901 ggagatgtcg aaacggcctg caacgagggg caacatgagc gccatagccg cgatggctgg
      961 gacggcctga gtggtcagga tggcgcgcag gttggcgaca ctcgcgaacg tatcggggag
     1021 tgcgagtgag aatccgacga tcaggatcag gaacaacaca ggcagggcat agcgttccgc
     1081 cagccccggc tccgatcgac cggcggtggc ctgcccctgc ctcggttggg atgccgcctg
     1141 aaccgcggat tcgccatcgc tcttgttggt cacgctcatc aagatgagac ccctgagccc
     1201 atgagtacct cctcgttgat tgcccgtcga ttgatgtctt tgcccgtcag ctcagtcacc
     1261 aatcgcccgt tgttcaggac caatacccgg tcgctcatat ccgcgagctc ctcgaagtcg
     1321 gaactcacga tcacgacagc aagtccgtcg ttggccgcct cgcggatgag cgcgaacaca
     1381 tctgctctgg cgccgacatc gacaccttgg gtcggctcat cgagcagcaa gatcgccggc
     1441 tttcgctcga gccagcgagc caaaaccacc ttctgttggt tccccccgga cagggtggag
     1501 attgcggctt ccgacgacgg tgccttgatg gagtacttct ccacgacatc gtccgagtcc
     1561 gaccgttcct tggcgcggtc gagcaacggc ccgcgtgcgt atcgactgac gctgggcgcg
     1621 ctgaggttct ccctcacgga gaggctggga aacacggctg ccgaccgctc ctcggggacg
     1681 taggcgacac ctcgggctat tgcggcgccg ggggtcgcgt tttcgaggtt ggtgcccaga
     1741 acttcgacct ggccaccatc cgttcgcatg tcaccgaata tcgtccgaag cagctccgac
     1801 cggccggagc cagtcagccc agcaatcccc aatacctcgc cctcccttac gtccaggctc
     1861 acatctcgta gtggcccggc cgtcacgctc ctgagacgtg ccacgacgct gccaggggtg
     1921 cgtgtccgct tctgccgtgc cagctcgccg aactcctcac cgaggatcaa ttcgaccagg
     1981 tcggattgtg tgagctcgcc ggccggctgc gaagcggcca acaggccatc ccgtagaacg
     2041 gtgaccgtgt cggcgattgc cctgacttcg tcgagtctgt gggtaacgaa gacgattgcg
     2101 acgccgtccc gagcgcacga tttgatggcc tcgaggagca catcgacttc ttcttccggc
     2161 attgcggccg tgggctcgtc cagcacgagc gtggatatcg ccgaggcctc tgtttcgtat
     2221 cggtcctgca acgcacgggc aacggcgatc atcgcctggt cggccggccg gaccgaggac
     2281 atgggttgat ctggcttggc ggtgatgccg aatcggtcga gaaggtcctt ggtcttggtg
     2341 cgaagggccc gccatctgac gaagccgacc acggtggggt acccatgacc caaggccaga
     2401 ttctcttcga cggtcatggt cccgaaggtc gtggagtgtt ggtggacgaa tctgagcccg
     2461 tggtgacgcg accatgccgg tgtcaccttg tcagcgtcga tcgattcacc cccgacgact
     2521 atttccccgc cgctatccgc gggcgtgact ccggcgagac acttgatcag ggtcgacttg
     2581 ccggagccgt taccgcccag gagtgcgtgc accttggctc tctccagtcc gaaagacacg
     2641 tcgcggagcg cggggcctcc cgcgtaggtc ttggacagtc gccggacttc aagggagatg
     2701 tccccccggt tcgaaccggg gggcatgtct cccgttgagg aggtcattcg gactcatccc
     2761 tcccagatgg ctgtgtaggc cgtccggaag tcgaccggag gggagtagga ctcgccctcg
     2821 ggcgggatgt tgtgctcggc gtcgacgaac tgccagcctc ctccctgatt tggcagtgtc
     2881 gccgggtcct cgccagcgaa gatccggttg agggagtcgg ccagacccca gaagatccac
     2941 tcgt
//
LOCUS       DASUTA010000031         3336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_11633, whole genome shotgun sequence.
ACCESSION   DASUTA010000031 DASUTA010000000
VERSION     DASUTA010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11633"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VFZ80_00620"
     CDS             <1..981
                     /locus_tag="VFZ80_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6285962.1"
                     /translation="EGMLFTEVQEKHGEELAEAIRTKTLPMGDTGESLQQAAKRAIGA
                     VDRLFDRMSDNERVAVVTHGGFMQPLLNRHLAGEGRRVHAFSANTGITRIVQQFGHAR
                     LASFNDVGHLGARPAALDAYLDSGDRVLTLIRHGQTQANVEGRWQGRGDWDLDEIGHN
                     QAEALGEWYGRHRTVYTSPLKRATSTARRVALNGSVAIDDLMEIHMGEWEGLTTDEIM
                     ERWPEDMETIYRHGIDLPRGGSGETWAQLAERVGLAIAGLDHDPDGHTIAVAHGGAIR
                     AYVSSLTSTTDTYSESLFTPANTSVTHIALTSRGPEILDYSVSTHLESMQ"
     gene            990..1574
                     /locus_tag="VFZ80_00625"
     CDS             990..1574
                     /locus_tag="VFZ80_00625"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6285963.1"
                     /translation="MTTYDSRRYALINRWDPSHLQRIDRLASIQPGDRVLEVGCGQGH
                     LTKALAARGIDVVGIDANPQAEEVASSPLVQHMKAEALDFDDASFDFVVSVHAIEHIP
                     ELDAALAEMSRVLRSNGRAVFIYPAEPIMGLYAIPTSIILHGTPLKAREVHCHKLWPS
                     KLRGMVEPLGLRQVSSEFSLLWSPQFASAFEKTG"
     gene            1578..2228
                     /locus_tag="VFZ80_00630"
     CDS             1578..2228
                     /locus_tag="VFZ80_00630"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HEX6285964.1"
                     /translation="MFTCETCGKEFDLAQHVLDRYPGWTPRQCLACRNSDPPRSGYTA
                     AAANSGSSVSKRRDLTLAEVLATFHDGPQTGVFTDGASEGNPGPGGWGAVLVVDGQVV
                     AQDYGSEGHTTNNRMELTAMIAGLEMLPSDTPTNVYTDSQLIVNILTQWAPGWESRGW
                     KKKSPGPIANLELVKKAYALTKSKPNAHIQWISAHSGNRWNEYADSLATAYRRQTL"
     gene            2231..3223
                     /locus_tag="VFZ80_00635"
     CDS             2231..3223
                     /locus_tag="VFZ80_00635"
                     /inference="COORDINATES: protein motif:HMM:NF021599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HEX6285965.1"
                     /translation="MPPPLTADQARAWNRYADTSPLYQRLNEVIADDSRLLDVLNSIE
                     RTPRHNVLFAGVQFLMMRDGAGPLADYYPNFADSPKDPRGVAVPFTDFVLGQASELIA
                     IGQTRYTQTNECRRCVGLLPAIWATGVTHFHLVDFGTSAGLNLHLDRYRYRWNSVSWG
                     PDQSPVDLFTELRGGEVTPLHMGILSRTGLDLNPLDPSDPDHRMWLEALVWPEHHDRR
                     RRLQTALDVAAAHPVDLIAGDGLVTLPAVLERIPAREPALVINSFILNQLSPDDRDRY
                     QRILASERARRPVYHVSMEWLDDSADSADIEVDDGTGLRRIGSAQPHGEWLELE"
BASE COUNT          655 a         1063 c         1029 g          589 t
ORIGIN      
        1 gaaggcatgc tgttcaccga agtccaggag aagcatggag aggagctggc ggaggcgata
       61 cgcaccaaga cactgcccat gggggacacc ggagagtctt tgcagcaggc ggccaaacgg
      121 gccattggag ccgtcgatcg gcttttcgac aggatgtccg acaacgagcg agtagccgtg
      181 gtcacccatg gtggcttcat gcagcctctg ctcaacagac atcttgccgg agaggggaga
      241 cgtgtccacg cattctccgc caacacgggc atcacccgaa tcgtgcagca gttcggacat
      301 gcccgcctcg cctctttcaa cgacgtgggg cacctcgggg cgagaccagc agcattggat
      361 gcgtatctcg actccgggga ccgcgtcctc acgctcatcc gacacggtca gacacaagcc
      421 aatgtcgaag gacgatggca gggtcgggga gattgggacc tcgacgagat cggccataat
      481 caggccgagg cgttggggga gtggtacggc cggcatcgga ccgtctacac ctctccgctg
      541 aagcgggcga cgtccaccgc gaggcgggtt gccctcaatg gatcggttgc aatcgatgat
      601 ctcatggaga tccacatggg ggagtgggag ggtctcacca ctgatgagat catggagcgc
      661 tggcccgaag acatggagac catctaccgc catggaatcg atcttccccg cggaggaagt
      721 ggtgagacat gggcgcaact ggccgaacgc gtcggtctcg ccatcgccgg cctcgatcac
      781 gatcccgacg ggcacacgat cgcagtcgcc cacggtggcg ccatcagagc ctacgtgtcg
      841 tctctgacca gcactacgga cacatactca gagtcgctgt tcaccccggc caacacctct
      901 gtcacccaca tcgcgctcac ctcgcgaggc cctgagatcc tcgactactc ggtgtcaacg
      961 catctcgagt cgatgcaata gcctccgcca tgaccacata cgattcacga cgctacgccc
     1021 tgatcaatcg atgggatcct tcccacctgc agcgaatcga ccgtctggca tcgattcagc
     1081 cgggcgaccg ggtcctcgaa gtcggttgcg gccagggcca tttgacaaag gcattggccg
     1141 cacggggaat cgacgtcgtc ggaatcgacg ccaaccccca ggccgaagag gtggcgagtt
     1201 cgcccctcgt ccagcacatg aaggccgagg ctctcgattt cgacgatgcc tcgttcgact
     1261 tcgtggtgtc ggttcatgcg atcgagcaca tcccggagct cgatgccgcc ctggcggaga
     1321 tgtcccgtgt ccttcgcagc aacgggaggg cggttttcat ctacccggcg gaaccgatca
     1381 tggggctgta tgccatccca acctcgatca tcctccatgg cacgcccttg aaagcgaggg
     1441 aggtccattg tcacaagctg tggccctcca agcttcgagg catggtcgag ccgctcgggt
     1501 tgaggcaggt gagctccgag ttcagcctgc tgtggtcacc tcagttcgcc tcagcgttcg
     1561 aaaagaccgg atagtcggtg ttcacctgtg agacctgtgg caaggagttc gacctggccc
     1621 agcacgtcct cgatcgatac ccgggatgga cgccgagaca atgcctcgcc tgtcgcaatt
     1681 ccgatcctcc ccgatccgga tacactgccg ccgcggccaa ctcgggatcc agtgtctcga
     1741 aacggaggga cctgacgctg gccgaggtgc tcgccacgtt ccacgacggc ccccaaacgg
     1801 gagtgttcac agatggggcg tccgagggga acccagggcc gggcggctgg ggcgccgttc
     1861 tcgtcgtcga cggtcaggtc gtcgcccagg actacgggtc ggagggccat acgaccaaca
     1921 accggatgga gctgaccgcg atgatcgctg ggctggagat gctgccatcc gacacaccga
     1981 ccaacgtcta taccgactct cagctgatcg tcaacatcct gacccagtgg gctcccgggt
     2041 gggagtcgcg cgggtggaag aagaagtcac cgggcccgat agcaaacctg gagctggtga
     2101 agaaggcgta cgccttgacg aagtcgaaac cgaacgctca catccaatgg atcagcgcac
     2161 acagcgggaa tcgatggaac gagtatgcgg actccctggc cactgcctac cgcaggcaga
     2221 ccctctgagc gtgccgccac cgctgactgc tgaccaggcg agagcctgga accgatatgc
     2281 ggacacatct cctctctatc agcggctcaa cgaggtgatt gcagacgatt cgcgcctgct
     2341 cgacgtcctc aactcgatcg agcgcacacc cagacacaac gtcctcttcg ccggcgtgca
     2401 gttcctgatg atgcgggacg gcgctgggcc gctggctgac tattacccca acttcgcgga
     2461 cagccccaag gaccctcggg gggtggcagt tcctttcacc gacttcgtct tggggcaggc
     2521 cagcgagctg atcgccatcg gacagaccag gtacacccag accaacgagt gccgccggtg
     2581 cgtcggtctg ctcccggcga tttgggcaac gggggtgacg catttccatc tcgtcgactt
     2641 cggcaccagc gcagggctca acctccacct cgacaggtat cggtatcggt ggaactcggt
     2701 ttcgtggggt ccagaccagt cgccagtcga cctgttcaca gagttgcgag gtggcgaagt
     2761 gactcctctc catatgggga tcttgagccg aaccggcctc gacctgaatc cgctcgaccc
     2821 atcagacccg gaccaccgga tgtggctcga ggcgctggtg tggccagagc atcatgatcg
     2881 gcgccgccgt ctccagacgg cgctggatgt ggccgctgct catcccgtcg atctcatcgc
     2941 tggggacggc ctggtcacct tgcctgcggt cctggagcga attcccgcca gagagcctgc
     3001 cctggtgatc aactccttca tcctgaatca gctctcaccg gacgaccggg atcgctacca
     3061 gcggatcctg gcctccgaac gggcccgtcg gcccgtctat cacgtctcga tggagtggct
     3121 ggacgattcc gctgactccg ctgacatcga ggtggacgat ggaacggggc tgcgacggat
     3181 cggcagtgct caaccacacg gagagtggtt ggagctggag tgaaacgtct ctatgcccga
     3241 ccgtagaccg ggatcagggc gccgttcatg accccagact cgtcactgag cacgaactcg
     3301 gcgacggctg cgatctcgtc gggagtcggc cagtcg
//
LOCUS       DASUTA010000032         3402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1695660, whole genome shotgun sequence.
ACCESSION   DASUTA010000032 DASUTA010000000
VERSION     DASUTA010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695660"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="VFZ80_00640"
     CDS             complement(<1..1174)
                     /locus_tag="VFZ80_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012184175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX6285966.1"
                     /translation="MFDLARKAVEGALDAGATYADARVVVRSHEQVAARNGEIEATVK
                     EEDAGIGVRALIGSSWGFAATSHLGDGEARSTGERAAAIARATGRVTGGDLVLADVAT
                     SQTSWATPFEEDPFSVSLSEKADLLIAVTSEMHTVEGVEVATGTLQFWDTDKWFVSSQ
                     GSEIHQHIVESGGGMDAMAVGESETQRRSYPQSFGEYQTGGYEVIRGFDLPAHAARLA
                     GEAVELLSADQCPSGDYDLVLEGSQLALQIHESVGHAIELDRILGWEAAFAGTSFLNL
                     EELGTLRYGSELMNITADATIPGALGTFGFDDEGTPAQSVDIVEAGRWVGVLSGRDSA
                     HIAGLPPGGMMRADGFARMPMVRMTNVGLKPGDSSLEEIVSETKRGVLMEVNRSWSI"
     gene            complement(1251..1703)
                     /locus_tag="VFZ80_00645"
     CDS             complement(1251..1703)
                     /locus_tag="VFZ80_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285967.1"
                     /translation="MTYRDVLLVLHIAGAGTWLGANVVQALVPPIAARHSAAVLAGWY
                     RITSAMATRLYVPAALLILLTGVLMVLDNEAYGFGTLFVTIGFGMIVVGALLGQFVFG
                     PGGDAAADAVESGDGGAIRSSVGRLTRYGLIDTLLLLFTITAMVLRLG"
     gene            1758..2135
                     /locus_tag="VFZ80_00650"
     CDS             1758..2135
                     /locus_tag="VFZ80_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285968.1"
                     /translation="MSKGTEAPGCRAAAISTAILVGVALVFMAIGLTLVNDSACSGPC
                     ETLGLTLLYAGLPISAAFGVLFGDLVVAWPLDITFWVVNAFFIARFSGNRGRRVFGPL
                     LVAILLSLAYGLVLSQFVELAIS"
     gene            2166..2513
                     /locus_tag="VFZ80_00655"
     CDS             2166..2513
                     /locus_tag="VFZ80_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285969.1"
                     /translation="MQGVITQRLAGLGLAAILTLTAAGGAVAVFSAGGPGEPLRPRGS
                     AETLIDLTQVRESILWSSLAGNVALVQTTGWPSSDGTNELGGALVIEAPTNPLAGTIN
                     IGEKPMSVEFSFD"
     gene            complement(2680..>3402)
                     /locus_tag="VFZ80_00660"
     CDS             complement(2680..>3402)
                     /locus_tag="VFZ80_00660"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX6285970.1"
                     /translation="RYGAGSLVIAAAGSLEHQTVVDMVAERFGDWSGDGVDHHFSAVE
                     IEPEVNLTRRETEQAHLVLGGPGLNRSDERRWAFEVLNHVMGSGMSSRLFREVREERG
                     LAYAVYGFKLSYADNGAWGVHVGTTPSQTDTALNVIREELAKVVDDGITTEELDRAKG
                     SMRGGLALALEDANSRMVRLGRDELAGMPHLSVDERLEKLDGVTLDMVKDVAGEIYGA
                     PTRVMGAVGPFEVDELDDYLTV"
BASE COUNT          608 a         1145 c         1055 g          594 t
ORIGIN      
        1 cgatcgacca cgaccggttc acctccatga gcacgccgcg cttggtctca gagacgatct
       61 cttcgaggct ggaatcaccg ggcttcaacc cgacgttggt catccggacc atgggcattc
      121 gagcgaagcc gtcggcgcgc atcatgcctc caggcggaag gccggcgatg tgtgccgagt
      181 cgcgcccgga gaggacgccg acccaacgac ctgcttcgac gatatcgacc gattgcgctg
      241 gagtcccctc gtcgtcgaat ccgaatgtgc ccagggcgcc gggaatcgtg gcatcggcgg
      301 tgatgttcat cagctctgac ccataacgaa gggtcccgag ctcttccagg ttcagaaacg
      361 aggtgcctgc gaatgcggcc tcccagccga ggatcctgtc gagctcgatg gcatgaccca
      421 cagactcgtg aatctgaaga gccaactgcg atccttccag aaccaggtcg tagtcgccgg
      481 aagggcactg gtccgcggac agcagctcca ccgcttcccc ggcgagacgg gctgcgtggg
      541 cgggaaggtc gaacccgcgt atcacctcat agccaccggt ctgatactcg ccgaaggact
      601 gcgggtagga gcgccgctga gtctcagatt caccaaccgc catggcatcc atgcccccac
      661 ccgactccac gatgtgctga tggatctcgg atccttgcga cgagacgaac cacttgtcgg
      721 tgtcccagaa ctgcagggtc ccggtcgcga cctcgacgcc ctcgaccgtg tgcatctccg
      781 atgtcacggc gatcaacagg tcggccttct ccgacagaga caccgagaac ggatcctcct
      841 cgaagggcgt cgcccaggag gtctgcgagg tggctacgtc ggcgagaacg agatcaccac
      901 cggtgacccg gcccgtggca cgcgcgatcg ccgcggctct ctccccggtg gagcgagctt
      961 cgccatcgcc gaggtgactg gtagcggcga atccccaaga cgagccgatg agcgcacgga
     1021 cgccgattcc ggcgtcctct tccttgaccg tcgcctctat ctcgccgttg cgggccgcga
     1081 cctgttcgtg actccggacc acgacccggg catcggcata ggtggcgcct gcgtccaggg
     1141 caccttcgac ggctttgcgg gccaggtcga acatcgattg caccactgaa gccggctcct
     1201 tctgctcgat tcggccaggg accctgaccc tagaccgaca tgatgcgcgc tcagccgagc
     1261 cgcaagacca tcgcagtgat cgtgaagagg agcagcagtg tgtcgatcag gccgtaccgg
     1321 gtcagccgtc cgacgctgga gcggatcgcc cctccgtcac cggactcgac cgcatcggcg
     1381 gcggcgtccc cgccgggtcc aaagacgaac tgcccgagca gggcgccaac gacgatcatc
     1441 ccgaagccga tggtcacgaa gagagtcccg aagccgtatg cctcgttgtc gagcaccatc
     1501 aggacaccag tgaggaggat gagcagcgcc gccggcacgt agaggcgcgt ggccatcgca
     1561 gatgtgatgc gataccaccc ggcgaggaca gcagcggagt gacgcgccgc gatcgggggc
     1621 accagagctt gaacgacgtt ggctccaagc caggtcccgg cccctgcgat atgaagcacc
     1681 aatagcacgt ccctgtaagt catgagccca atctacgcga gtaccgcttc acatccgacg
     1741 tctcatacga tcgaatggtg tccaagggaa ccgaagcgcc gggctgtcgc gccgcggcga
     1801 tctcgacagc gatcctggtg ggtgtggcgc tcgtcttcat ggcgatcggg ctcacactcg
     1861 tcaacgactc cgcctgctcg gggccatgtg agacgctcgg tctcaccctc ctctacgccg
     1921 ggctccccat ctctgcggca tttggcgtcc tattcggtga cctggtggtg gcgtggccgc
     1981 tggacatcac cttctgggtg gtgaacgcct tcttcatcgc ccgattctcg ggcaaccggg
     2041 gtcgcagggt ctttggcccg ttgctcgttg cgattctcct ctccctggcc tatggactag
     2101 tcctttcgca gttcgtggaa ttggccattt cctgagggtc cttggtcact tagcgtggca
     2161 gacagatgca gggagtgatc acacaacgtc tcgccgggtt gggattggcc gcaatattga
     2221 ctctgacggc agcagggggc gctgtcgcag tgttttcggc cggggggccc ggcgagcccc
     2281 tgcgtccccg gggttccgcc gagacactca tcgacctgac ccaggtccga gagtcgattc
     2341 tctggagctc tctcgccggc aacgtcgcct tggtgcagac cacaggatgg ccctccagcg
     2401 acggcaccaa cgagctcggt ggcgctctgg tcatcgaggc acccaccaac ccactggccg
     2461 gcaccatcaa catcggcgag aagccgatgt cggtcgagtt cagcttcgat tagcgaccgt
     2521 tcgcatggca gtgccggtgg gcgcgagcgg cagctgcggc ccgacgggcg tctgttttcc
     2581 ggaagatctt cggtacggcg atattggtga tggaggaccc ccagcagcgc ttcaggtcga
     2641 tatcccgggg acaagccgga agagtcgggc cgccatagat cagaccgtca ggtagtcatc
     2701 cagctcgtcg acttcgaagg gaccgaccgc gcccatcacc cgggtggggg caccgtagat
     2761 ctcgccggca acgtccttca ccatgtccag ggtcacccca tcgagcttct cgagacgctc
     2821 gtccacggag aggtggggca tcccggccag ctcgtcgcga ccgagtcgca ccatccggct
     2881 gttggcatcc tcgagcgcta gggcgagacc accgcgcatc gaccccttgg cccgatcgag
     2941 ctcctcggtg gtgatcccgt cgtccaccac cttggccagc tcctcgcgga tcacgttcaa
     3001 ggcggtgtcg gtctggctgg gagtggtgcc gacgtgcaca ccccaggccc cgttgtccgc
     3061 ataggagagc ttgaagccgt agacggcgta ggcgagaccc cgctcctcac gcacctcgcg
     3121 aaacagccgc gaggacatgc cggagcccat gacgtgattg agcacctcga aggcccaccg
     3181 tctctcgtcg ctgcgattca aacccggacc tcccagcacg agatgggcct gctcggtctc
     3241 ccgcctggtc aggttgacct ccggttctat ctcgaccgcc gagaagtggt gatcgacacc
     3301 gtcgccggac cagtcgccga agcgctccgc aaccatgtcc acgaccgtct ggtgttccag
     3361 cgacccggct gccgcaatca cgagagaccc ggctccatag cg
//
LOCUS       DASUTA010000033         2475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1437651, whole genome shotgun sequence.
ACCESSION   DASUTA010000033 DASUTA010000000
VERSION     DASUTA010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437651"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFZ80_00665"
     CDS             <1..216
                     /locus_tag="VFZ80_00665"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX6285971.1"
                     /translation="HPGWAYNLVKRSDSVTVDAGHGEVPVSIEMLSGAERERTWRRIA
                     DTSPGYGRYEEKTDREIPIFRLTPHAD"
     gene            206..652
                     /locus_tag="VFZ80_00670"
     CDS             206..652
                     /locus_tag="VFZ80_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017578839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX6285972.1"
                     /translation="MPTDFVPDDWEPPTSFEGPGFRLEPLGPTHNERDFEAWSSSMDH
                     IHATPGEWGDWPRPMSLEENLSDLEGHAREFVEREAFTYSILDDNDVIGCLYIYPDEK
                     GASAAYVSSWVTESRAEMDAIVWKAVSDWLSNDWPFEGFRYAERPG"
     gene            727..912
                     /locus_tag="VFZ80_00675"
     CDS             727..912
                     /locus_tag="VFZ80_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285973.1"
                     /translation="MRDVADRYAPSLDQVVERLTRLARSLQSEGLEEQASSLETTIAD
                     LQWQCEVLDSVELEPAR"
     gene            992..1273
                     /locus_tag="VFZ80_00680"
     CDS             992..1273
                     /locus_tag="VFZ80_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285974.1"
                     /translation="MSIAHASVDDPDAQYIFGAWSNMVVGQRPDGLIDCYLSQGESAV
                     YMVSVWEDGAAYDRAMGDRESHPDYGFFEACGLEPTNTIYDVIGRIAPR"
     regulatory      1332..1457
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            1463..>2475
                     /locus_tag="VFZ80_00685"
     CDS             1463..>2475
                     /locus_tag="VFZ80_00685"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX6285975.1"
                     /translation="MEPFAGISHEDLLKLVFSFAILLGSARLLGGLAKRMGLPSVVGE
                     LLAGVVLGPSLLSGIFPAVGALIVPSTEVQAQLLDVVGLFGVMFLLMVVGLETDISLI
                     KSRARVATAVGLAGLIVPFAAGLGVSTLFPDDLLVDPERRRVFALFLAVALALSAIPV
                     LAKVLSDMRLIRTPFGQSTLAAGMIDDILGWTLLGIVTSLAAAGGVNAGNVAATIVAV
                     VVFLAATLFVARPLARVSLRILQERQRARDATLTLLVVSCFAWGAFSHALHLEPVLGA
                     FAIAVVFGQIRRLPSEVGRSLEGITFGVFAPVFLAIAGLRLSLDTIAEPALVTLTVIL
                     LVVA"
BASE COUNT          412 a          764 c          791 g          508 t
ORIGIN      
        1 catccgggat gggcttacaa cctcgtcaaa cgctcggatt cggtgacggt ggatgccggt
       61 cacggtgagg tccccgtctc catcgagatg ttgtcgggtg ccgagcgcga gcggacttgg
      121 cgacgcatcg ccgacacctc accgggctat ggaaggtacg aggagaagac cgaccgcgag
      181 atccccatct tccgcttgac acctcatgcc gactgacttc gtccctgacg attgggagcc
      241 accaacatcc ttcgaaggac ccggatttcg cctcgagcca ctgggcccga cccacaacga
      301 gagagacttc gaggcgtggt cgtcgagcat ggaccacatc cacgcaactc cgggagagtg
      361 gggcgattgg ccccgtccga tgtcgttgga ggagaacctc tccgatctcg agggtcacgc
      421 ccgcgagttc gtcgaaaggg aggcgttcac ctattcgatc ctcgacgaca acgacgtcat
      481 cggttgccta tacatctatc ccgacgagaa gggcgccagc gccgcctacg tcagctcgtg
      541 ggtgacggag agccgtgccg agatggacgc cattgtctgg aaggccgtct cggactggct
      601 ttcgaatgac tggccattcg aaggattcag gtacgcggag cgaccgggct gagttgtagc
      661 agacggtttc ggagcccgat gcggcggggt agatagtgct ggtcgacgtc aaacaggacg
      721 gatttcatga gagatgtagc agatcgctat gcaccaagtc tcgaccaggt cgtggaacga
      781 ctcacccgcc tggcccgctc cctgcaatcc gaggggttgg aggaacaggc gagcagcctg
      841 gagaccacca ttgccgactt gcagtggcag tgcgaggtgc tcgactcggt cgagctcgaa
      901 ccggctcgct agatcggtcc aacgcgttgc ttccgtgtct tcggggttgt atacagagtc
      961 cggcctgagt cgagaggagc tcgcgttgat catgtcaata gcccatgcgt cggtggacga
     1021 ccctgatgct cagtacatct tcggggcgtg gtcgaacatg gtggtcgggc aaagaccgga
     1081 cggtctgatc gactgttatc tgtcacaggg tgagagtgcc gtctacatgg tctctgtctg
     1141 ggaggacggc gcggcgtacg accgggccat gggagacaga gagagccatc cagactatgg
     1201 cttcttcgaa gcttgcggat tggagccgac gaacaccatc tacgacgtga tcggacgcat
     1261 cgctccccgc tgaacggccc gttccccgtt atcgagaagc gcgcaatcga gattacgatc
     1321 tagcgcgcaa cgccgagccc gggtcgaatc cggggcgggg gacccaacaa cctggggcga
     1381 atcccggccg tcagccgggt agagcacctt tcagctcgag cccgtcagct aaccccgcag
     1441 gccaggtgga aggaggtggt cggtggagcc gttcgctggc atatcccacg aggatctact
     1501 caagctggtt ttctcgttcg cgatcctcct gggcagcgcc cgtctgctcg gcgggctggc
     1561 gaaacggatg ggccttccct cggtggtggg cgagctgctc gccggcgtcg tgctgggacc
     1621 gtccctcctc tccgggatct ttcctgcagt aggggctctg atcgtgccga gcaccgaggt
     1681 tcaggcccag ctgctcgatg tcgtagggct cttcggggtg atgttcctgc tcatggttgt
     1741 gggtctcgag accgacatca gcctcatcaa gtcgcgggca agggtggcga ccgcggtcgg
     1801 cctggcgggt ctgatcgtcc cgttcgccgc cggtctcggc gtgagcacct tgtttcccga
     1861 tgatctgctt gtcgacccgg agcgtcgacg agtcttcgcc ttgtttctgg cagttgcgct
     1921 cgcgctctcg gccattccgg ttttggccaa ggtcctttcc gacatgcggc tgatcagaac
     1981 cccgttcggt caatcgacct tggccgcggg gatgatcgat gacatcctcg gctggaccct
     2041 gctcggcatc gtcacctccc tcgcagcagc agggggtgtc aatgccggca atgtggcggc
     2101 gacgatcgtc gcggtcgtgg tcttcctcgc cgccaccctc tttgtggccc gtccgctcgc
     2161 tcgcgtctcc cttcgcatcc tccaagagcg gcagcgagcg cgtgacgcga cgctgacgtt
     2221 gctcgtcgtc tcctgtttcg cctggggcgc tttcagccat gccttgcatc tcgagccggt
     2281 cctgggcgcc tttgccatcg cagtggtgtt cggccagatt cgccgactcc cctccgaggt
     2341 gggacgaagc ctggaaggca tcacattcgg tgtgttcgca cccgtgtttc tcgccatcgc
     2401 cggtctgcgt ctgtccctcg acaccattgc cgaaccggct ctggtgacac tcacagtgat
     2461 cctcctggtc gttgc
//
LOCUS       DASUTA010000034         3391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_790909, whole genome shotgun sequence.
ACCESSION   DASUTA010000034 DASUTA010000000
VERSION     DASUTA010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790909"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VFZ80_00690"
     CDS             <1..710
                     /locus_tag="VFZ80_00690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003564962.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX6285976.1"
                     /translation="REISLLLRRPPGREAYPGDVFYLHSRLLERCAKLSDELGAGSLT
                     GLPVIETKADDISAYIPTNVISITDGQVFLKTDLFFQGIRPAVDAGQSVSRVGGNAQI
                     NAMRKIAGPLRLNLAQFRELAAFAEFGSELDAASLAQLERGRRVVEVLKQPQFSPIPV
                     EEQVLALFAVTEGHFDDIPTEDIRRAERELREYFRTRRGDVLSGIRDTGKLPEGDELA
                     EGIKAFKETFETSEVEE"
     gene            725..1609
                     /locus_tag="VFZ80_00695"
     CDS             725..1609
                     /locus_tag="VFZ80_00695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004145.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX6285977.1"
                     /translation="MPSAALRDTRRRISSVEATKKITRAMELIAASRIPKATARVEGS
                     KPYTAKLIEVIENVGGANAGASHMLLERRELSTVGILVVSSDRGMAGAYASNIIRLAE
                     QRIAELQKVGTDVLIYAVGKKAQSYFRYRGYRIEQAYLGVTDTPGYNNARAIANTIME
                     AYASQRIDALEAFYTRFQSAMTQSPVRTEILPITPPEKGEGEGAPVSYEFEPEPGEIL
                     DRLLPRYVEAVIFNMLLEASASEHSARRRAMKAATENAEELIKRLTVQANRARQAEIT
                     TAITEIVGGANALAETKR"
     gene            1710..3137
                     /gene="atpD"
                     /locus_tag="VFZ80_00700"
     CDS             1710..3137
                     /gene="atpD"
                     /locus_tag="VFZ80_00700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX6285978.1"
                     /translation="MTDSNAAVGRIVKVAGPVVDVEFPPDELPEILNAVEIFFTLGGE
                     DKKVIAEVAQHLGGSKVRAIAMAPTDGMTRGAEVRDTGSPITVPVGDQTLGHIFNVWG
                     DSLDAPDMDFSEPRWPIHRTPPKFEDVEPQKTVFETGIKVLDLICPYLQGGKIGLFGG
                     AGVGKTVLIQEMINRVATQHGGVSVFAGVGERTREGNDLFLEMQESGVIDKAALCFGQ
                     MDEPPGVRLRVALSALTMAEYFRDVQGQDVLLFIDNIFRFTQAGSEVSTLLGRMPSEV
                     GYQPNLADEMGSLQERITSLKGRSITSLQAIYVPADDITDPAPHTAFAHLDATTVLSR
                     PLTALGIYPAVDPLDSSSRALDPQIVGQEHYDVAVEVQRILQRYKDLQDIIAILGIDE
                     LSEEDKLVVGRARRIQRFLAQPMFVAEQFTGQEGIYTPREETISSFKALIDGEMDRFP
                     EQAFYMVGGREDVERRAAEMSKDAA"
     gene            3141..>3391
                     /locus_tag="VFZ80_00705"
     CDS             3141..>3391
                     /locus_tag="VFZ80_00705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009977.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX6285979.1"
                     /translation="MAKAFQVDVVSPEATVWSGQATIVIARTPEGELGIMADHEPLMG
                     ALATGPVEIESVSGERTTIGVHGGFIQVLDNQVTLITDRA"
BASE COUNT          645 a         1094 c         1070 g          582 t
ORIGIN      
        1 atcgtgagat ctcactgttg ttgcgacgcc cgccgggtcg tgaggcatat ccgggagacg
       61 tcttctacct gcacagccgt cttctcgaga ggtgcgccaa gctctccgac gagctcggcg
      121 ccggctccct gaccggtctc ccggtcatcg agaccaaggc cgacgacatc tcggcgtaca
      181 tcccgaccaa cgtgatctcg atcaccgatg gccaggtctt cttgaagacc gacctgttct
      241 tccagggcat tcgtcccgca gtcgacgccg ggcaatcagt ctcccgggtg gggggtaacg
      301 cacagatcaa cgccatgcgc aagatcgccg gcccgctgcg tctcaacctg gcgcagttcc
      361 gggagttggc tgcattcgcc gagttcgggt ctgagctcga cgccgcatct ctcgcacagc
      421 tcgagagggg acggcgtgtc gtcgaagtgc ttaaacagcc acagttctct ccgatcccgg
      481 tggaggagca ggtgctcgca cttttcgccg tcacggaggg tcacttcgac gacatcccca
      541 ccgaagacat ccggcgtgcc gagcgcgagc tgcgcgagta cttcaggacg cgtcgcggcg
      601 atgtcctgtc cggaatccgg gataccggga agctacccga aggtgacgag ctcgccgagg
      661 ggatcaaggc cttcaaagag acattcgaga cctcagaggt cgaggaatag gtgagctgac
      721 ctagatgccc agcgccgcgc tccgtgacac ccgccgccgg atctccagcg tcgaggcgac
      781 gaagaagatc acgcgcgcca tggagctgat cgcggccagc cgaatcccca aggcaaccgc
      841 tcgagtcgaa ggctccaagc cctacaccgc caagctgatc gaagtgatcg agaacgtcgg
      901 cggggcgaac gcaggtgcct cccacatgct gctggagcgg cgcgagctct cgaccgtggg
      961 aatcctggtg gtctcctcgg accgggggat ggcgggagcc tatgcctcca acatcatccg
     1021 tctcgccgag caacggatcg ccgagcttca gaaggtcggc accgacgtcc tcatctacgc
     1081 agtcggcaag aaggctcagt cctatttccg ctaccgcggc taccggatcg agcaggcata
     1141 tctgggagtg acggacacac cgggctacaa caacgccagg gccatcgcca acaccatcat
     1201 ggaggcctat gcctcgcagc ggatcgacgc cctcgaggcc ttctacacga ggttccagtc
     1261 ggcgatgact caatcgcccg ttaggactga gatcctcccg ataactcccc cggagaaggg
     1321 ggaaggcgag ggtgctccgg tgtcctacga gttcgagccg gaaccgggcg agatcctcga
     1381 ccggcttctt cctcgctacg tggaggccgt gatcttcaac atgctgctgg aggcgtcggc
     1441 ctccgagcac agcgcccgtc gccgcgccat gaaggcggcg accgaaaacg ccgaggagct
     1501 gatcaagagg ctgaccgtcc aggccaaccg ggcccggcag gccgagatca ccacggccat
     1561 caccgagatc gtgggtggcg ccaacgccct ggcggagacc aagcgatgac cgaatcacac
     1621 cgtttagacc gttctgtgaa cgctggtggt cactatgtga ccatccgcgt tcacagaacg
     1681 gttgaagacc tgataactgg agcaatcaga tgactgacag caacgcagcc gtagggcgga
     1741 tagtcaaagt ggcgggcccg gtcgtggacg tcgagttccc gcccgacgag ctgcccgaga
     1801 tcctcaacgc ggtggagatc ttcttcacgc tgggtggaga ggacaagaag gtgattgccg
     1861 aggtcgccca gcatctcggt gggtccaagg tgcgcgccat cgccatggct ccaaccgacg
     1921 ggatgacaag aggcgctgaa gtccgtgaca ccggctctcc gatcaccgtt ccggttggcg
     1981 accagacgct tgggcacatc ttcaacgtct ggggcgattc tctcgatgcg ccggacatgg
     2041 acttctccga gccgcgttgg ccgatccacc gcacacctcc gaagttcgag gacgtcgagc
     2101 cgcagaagac ggtcttcgag acggggatca aagtcctcga cctgatctgt ccctacctcc
     2161 agggtgggaa gatcggtctc ttcggcggtg ccggcgttgg gaagacggtg ctcatccagg
     2221 agatgatcaa ccgggttgcc acccagcacg gtggtgtctc ggtgttcgcc ggtgtcggcg
     2281 agcgcacccg tgaggggaac gacctctttc tcgagatgca ggagtcggga gtcatcgaca
     2341 aggccgcgct ctgcttcggt cagatggatg agccgccggg ggtccgtctc cgggtggcac
     2401 tctccgctct gaccatggcc gagtatttcc gtgacgtaca aggccaggac gtgctgttgt
     2461 tcatcgacaa catcttccgc ttcacccagg ccggctccga ggtctcgact ctgctcggcc
     2521 gcatgccgtc ggaagtgggg tatcagccca acctcgccga cgagatgggc tccctccagg
     2581 agcggatcac ctccctgaag ggtcgctcga tcacgtcgtt gcaggcgatc tacgttcccg
     2641 ccgacgacat caccgacccg gcgccacaca ccgcattcgc ccacctcgat gcgaccacag
     2701 tgttgtcccg tcctctcacc gccctcggca tctaccccgc cgtcgacccc ctcgactcgt
     2761 cgagccgcgc cctcgacccc cagatcgtcg gtcaggagca ttacgacgtg gcagtcgagg
     2821 tgcagcggat cctgcagcgg tacaaggacc ttcaggacat catcgccatc ctcggcatcg
     2881 acgagctgtc cgaggaggac aagctcgtgg tcggccgtgc ccgccgcatt cagcggttcc
     2941 tggcacagcc gatgttcgtc gccgagcagt tcaccggtca ggagggcatc tacacgccgc
     3001 gcgaggagac gatctcttcg ttcaaggcgc tgatcgacgg cgagatggac agattccccg
     3061 agcaggcctt ctacatggtg ggtggtcgcg aggacgtcga gagacgcgcc gccgagatgt
     3121 cgaaggacgc ggcatagcga atggccaagg cgtttcaggt cgatgtggtg tcgcccgagg
     3181 ccacggtgtg gagcggtcag gcaacgatcg tcatcgcccg gactccagag ggggagctcg
     3241 ggatcatggc cgaccatgag ccgttgatgg gcgccctggc caccggcccg gtcgagatcg
     3301 agtcggtgtc tggggagcgc accacgatcg gggtccacgg cggcttcatc caggtgctcg
     3361 acaatcaggt gaccctgatc acagaccgtg c
//
LOCUS       DASUTA010000035         2585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_142925, whole genome shotgun sequence.
ACCESSION   DASUTA010000035 DASUTA010000000
VERSION     DASUTA010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142925"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="VFZ80_00710"
     CDS             <1..1408
                     /locus_tag="VFZ80_00710"
                     /inference="COORDINATES: protein motif:HMM:NF039580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX6285980.1"
                     /translation="INMNFFPVGPLLRRTGVFFIRRSFRDNELYKIVLRAYLDYLIEK
                     RFPLEWYMEGGRSRSGRLNPPRLGLLHYVVDSLRRGKSEDIKLIPVSIAYDQIQDVPD
                     YAREAQGKGKESESLGWMLRAVKSLRRRYGDIYIRFGDPVSVASVLGSIDEDEEEGSL
                     GLQKLAFEVMYRIGRVTPTTPTAVVSIALLAARGQARTAEELARDCVELVDFIRARGL
                     PTTESLDIADGETLTNLLDWMAEHGNVSSHEALGRRVFWLEDEQMIRVSYYRNVVVHF
                     FVNRAIAEMAITSLLDEPGPSAGAARERMLALRDLLKFEFFFPEKDEYLELVSDDIEV
                     DVPGWNRLLASAGPSEVLAKMGDPIAYWALLPFLDSYQIVGDELEALEGPFEEKRFLQ
                     NCLARARMYRIEERVFSGESASQVLFKSALALARNRGLVDESPGVAMRRAEFAAEMRD
                     ARGMAARGLGQGLRSQRR"
     gene            complement(1381..1914)
                     /locus_tag="VFZ80_00715"
     CDS             complement(1381..1914)
                     /locus_tag="VFZ80_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285981.1"
                     /translation="MASMTALGRILQVIGWVWIAVGFLGPIVNLPSVSVFPGIIILFV
                     ARVIRKQGEQQTRPREESAEQAEDQVTPRPLNTQRSRPRLEPKKARQPSPAARTGPRA
                     EPTTEPGPVPSSRDAGPPADEREELLERVLLVGQELTDEPPEPLYPEQISDDDEGSPL
                     SSAEMIARAHRRWDRKP"
     gene            complement(1964..>2585)
                     /locus_tag="VFZ80_00720"
     CDS             complement(1964..>2585)
                     /locus_tag="VFZ80_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6285982.1"
                     /translation="SLFPASLGRFDLGAASQLVLFTFLTALAGSAALILTRQLGISQR
                     MLSTPTSLGTIVVGESVGRFGTAMVQGLYIIVLTLIIFGVNWGDPVGAILLLVAFAAV
                     GAAAGVLMGSVFSNDQQAGGVGVVLSIGLGALGGCMLPIELFSPTMQRVAHITPHAWA
                     LDGYAELVRRDGNVADILPELGVLVAYAAVLFVLASWRLRVAITRP"
BASE COUNT          492 a          825 c          823 g          445 t
ORIGIN      
        1 catcaacatg aacttctttc ccgttggccc ccttctccgc cggaccggag tgttcttcat
       61 ccgccgatct tttcgggaca acgagctcta caagatcgtg ctcagggcct atctcgacta
      121 tctcatcgag aagcgattcc cgctcgagtg gtacatggag ggagggcgct cacgatccgg
      181 gcgactcaat cccccgcgct tgggcctgct ccactacgtg gtcgactcgc tgcgccgggg
      241 caagagcgaa gacataaagc tgatcccggt gtccatcgcc tatgaccaga tccaggacgt
      301 gcccgactat gcgcgagaag cccaaggcaa aggcaaggag agcgaatcgc tgggatggat
      361 gctgcgtgct gtcaagtctc tgcggagacg atacggcgac atctacatca gattcggtga
      421 cccggtgtcg gtggcctcgg tcctggggtc gatcgacgag gacgaggaag agggatcact
      481 cgggcttcag aaactcgctt tcgaggtcat gtaccggatc ggcagggtga ctccgaccac
      541 accgacggca gtcgtgtcga ttgcgctgct agccgcccgc ggccaggccc gaaccgccga
      601 ggagctggcc cgagactgcg tcgagctcgt cgatttcata cgtgcccggg gccttccgac
      661 gacagagtca ctcgacatcg ccgatggcga gacgctgacc aatctcctcg actggatggc
      721 ggagcacggg aacgtgtcca gccatgaggc actcggtcgc agggtcttct ggttggaaga
      781 cgagcagatg atcagggtct cgtactaccg caacgtcgtg gtccacttct tcgtcaatcg
      841 ggccatcgcc gagatggcca taacctcgct cctggacgag ccggggcctt cggccggggc
      901 tgcaagggag cggatgctcg cactgcggga tctgctcaag ttcgagttct tcttcccgga
      961 gaaggacgag tacctcgagt tggtttcaga cgacatcgaa gtcgacgttc ccggttggaa
     1021 ccgtcttctc gcttcggcgg ggccatctga ggtgctggca aagatgggcg atcccatcgc
     1081 ttattgggcg ctgctcccat tcctcgacag ctatcagata gtcggcgacg aactggaagc
     1141 cctcgaggga ccctttgagg agaagcgatt cctccagaac tgtctggcca gggccaggat
     1201 gtaccgcatc gaagaacggg tcttctcggg tgagagcgcg agtcaggtcc tgttcaagtc
     1261 ggcgctcgcc ctcgcccgta accgtggact ggtcgacgag tcacccgggg tcgccatgag
     1321 aagagccgag ttcgccgccg agatgcggga cgcccgcggc atggccgccc gaggactggg
     1381 tcagggcttg cgatcccagc gacgatgagc acgggcaatc atctccgccg agctcagcgg
     1441 gctgccctcg tcatcgtctg agatctgctc gggatatagg ggctcgggcg gctcgtcggt
     1501 caactcctga cccacgagaa ggacacgctc cagcaactcc tccctctcgt cggcgggagg
     1561 cccagcgtcg cgggagctcg gaaccggccc tggctcggtc gtaggctcgg cccgaggccc
     1621 ggttcgcgcg gccggtgagg gctggcgggc tttcttcggc tcgagacgag ggcgtgaccg
     1681 ctgtgtgttc aggggtctcg gtgtcacttg atcctcggcc tgttcggcgc tctcttcccg
     1741 gggccgcgtt tgctgctctc cctgcttcct tatcacccgt gcgacgaaca ggatgatgat
     1801 gcccggaaag acacttacgc tggggaggtt cacgatcgga ccgaggaatc cgacggcgat
     1861 ccacacccac ccgatcactt gaaggatccg cccgagcgct gtcatggagg ccattcaacc
     1921 atcaagaccg gacattgggc catgcggcac catcccgccg cggtcaggga cgggttatgg
     1981 ccacccgaag acgccaggag gccagcacga acagcacggc tgcgtaagcc accagcaccc
     2041 cgagctcggg gaggatgtcg gcgacattgc cgtcgcgacg gaccaactcg gcgtacccgt
     2101 ccagcgccca ggcgtgcggt gtgatgtgcg ccactcgctg catcgtcggg gagaacagct
     2161 cgatgggcaa catgcaccca ccgagggcgc cgagcccgat gctcaggacc actcccaccc
     2221 ctcctgcctg ctgatcgttc gagaacaccg agcccatgag aacaccggcc gcagcaccga
     2281 ccgcggcgaa ggccaccagc agcaggatcg ccccaacggg atcaccccag ttgaccccga
     2341 agatgatcag ggtgaggacg atgatgtata gaccctggac catcgccgtc ccgaacctcc
     2401 cgacggactc gccgacgacg atcgtgccca gcgaggtcgg tgtcgacagc atccgctggc
     2461 tgattccgag ctgtctggtg aggatcaacg ccgccgaacc ggcaagcgca gtcaggaagg
     2521 tgaacagcac cagctgagag gcggcgccca gatcgaatcg tcccaaggac gccgggaaca
     2581 gcgac
//
LOCUS       DASUTA010000036         5692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_143373, whole genome shotgun sequence.
ACCESSION   DASUTA010000036 DASUTA010000000
VERSION     DASUTA010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143373"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VFZ80_00725"
     CDS             complement(<1..633)
                     /locus_tag="VFZ80_00725"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF025118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX6285983.1"
                     /translation="MTDQTFTVSQVLKGLQEAVVRAFPTPIWVRGEVTGLRRTSRGAV
                     FFRLADSDASENALEVAARGRVMMEVDRLLTESGLGSFRDGIEARVNGTIGVDERGSR
                     VRLSLLEVDPAFTAGRIALDRADVIRRMTVDGSLRANALLPIPMVPLQIGLVTSRGSA
                     AHADFIDQLRQSSFRFRVKTAHSAVQGEHAAAALASAIERVGGNDVDIVAV"
     gene            complement(630..1094)
                     /locus_tag="VFZ80_00730"
     CDS             complement(630..1094)
                     /locus_tag="VFZ80_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX6285984.1"
                     /translation="MSAPPGYDASRFPPFAVTADVALLTTVEGRLGVVLVQRSHDPYR
                     GRWALPGGFVDIDEDLEPAAIRELGEETGLQPGKLTQLGAYGAPGRDPRMRVVTVVFW
                     AHVADLGQPTSGDDAADARIWDLDDVLADPSMVAFDHHLILSDVQSTYEASR"
     gene            1141..3267
                     /locus_tag="VFZ80_00735"
     CDS             1141..3267
                     /locus_tag="VFZ80_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX6285985.1"
                     /translation="MGAQPAYPGRSGREPIFCAHMGNQADEAGPPSLEPAASTESRFD
                     RFSALVALTLFGLTWPVLDLLGRNAEFFLARRSAKAEILFVALTAIVAVPVAVGALGF
                     LPGRAGRVIALGLVAVTASSLALLYLARTELPWWLSTSVALGIGLGTTWAFVHVTPVR
                     QAVRYLVIAPLVMLGVFLFSMPVGAVLWEPDVALGNPVPIESPVPIVLVVFDEFPVAS
                     LIDPEGDLEADRFPNFGRLASDGTWYRNAVTVEQQTEQSVPAILTGRIPGDSLVPVAG
                     QYPFNLFTALRSVYDLHAHESITQLCPRQLCEGISATASSLVGDVSVVAGHVLLPEPL
                     SEDLPPIDRGWGDFATAAGTFNARQEFREVLSRGPRAAIDATLGDIEAADVSRPTLFY
                     MHAIVPHHPWQYLPDGRSYPFIVATNPASRGGGWNDDEFLVAQSMQRHLLQVGYADHV
                     LGEVIAALEKRGLYEDAMVIVVADHGITIRPGVDHQRMITEETIGSIAAVPLFVKTPN
                     GDGGVIDDRRALTIDILPTIADVIGASLPSDVEGLSLLQPPPDRTLTTTYGPEGQVTF
                     GVSGEEKLEVARAIFERFPNGDPWALRPLGAPDLAGSEIDRDSLSSSRLRVIVREPGL
                     YRSVDTEGAVIPVRIGGTLLGEAEGDEVLAVAVNGVIAAVTRAYEGDDAVAFLAMVPP
                     ERFRDGENVIDVLEVTNDGRLLRLDS"
     gene            complement(3295..4251)
                     /locus_tag="VFZ80_00740"
     CDS             complement(3295..4251)
                     /locus_tag="VFZ80_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018301339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX6285986.1"
                     /translation="MTDYVAGIKRAIIIDADPGQDDAVAILAALASPEELDVLAITTV
                     AGNVPIEKVTNNALALVELAGRSEVPVYQGAVRPMVNRLVTAEYVHGPTGLDGADLPP
                     PRLEISSGHAVDRLVELVMGRSEPTTVCTLGPLTNVALALVKEPDIAGKIDQIVLMGG
                     SFFEGGNTTPAAEFNIFVDPHAAHVVFTSGVPLVMMPLDVTHKALTTPDRLRRFTEIG
                     TTAGSSVAGMLRFYDRHDIEKYGMEGGPLHDPTVIVYLLSPDIFAGKEVAVEIETAPG
                     PTQGMTLVDWWGVTRSEPNALVMNEIDADRFYDILVELIGRL"
     gene            complement(4244..5146)
                     /locus_tag="VFZ80_00745"
     CDS             complement(4244..5146)
                     /locus_tag="VFZ80_00745"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016471608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEX6285987.1"
                     /translation="MADIVVVGSLNHDLTVITSSHPRPGETVLGRGHYWDNGGKGANQ
                     AVAVARMGGSVAMVGRVGDDLHGHGLRKALAAEGVDVEGVGFDRGAATGLAVITIDDA
                     AENTIVVSPGANARLTPEHIEAQHDQIAAGGVVLAQLEVPIDAVAAAARLVDGIFCLN
                     PAPATELPSEVLARTDVLIPNRGELGVLAGVTEPVSIDEVIAAARKLRIDAAIVVTLG
                     SDGALLLWDGEADRFPAPEVEAVDPTGAGDAFCGALADALGRDLSLEVAVERATAAGS
                     LATTTRGAQSSMPTSQQVERLLSD"
     gene            complement(5156..5629)
                     /locus_tag="VFZ80_00750"
     CDS             complement(5156..5629)
                     /locus_tag="VFZ80_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017327582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEX6285988.1"
                     /translation="MPTGYRYWLTRVWDVGQPPVSWVMLNPSTATETVDDPTIRRVVA
                     FSRLWGFGSALVVNLFALRSVSPETLARHPDPVGPENDSAIERAIGSADLVVVAWGNH
                     GLKLNPKTRTPRSSEVAALLSGVNPRCLGLTASGQPRHPLYLPSCTASMAFPRPT"
BASE COUNT          976 a         1846 c         1838 g         1032 t
ORIGIN      
        1 gacggcgacg atgtctacgt cgttgccacc cacccgttca atggcgctgg ccagagccgc
       61 tgcagcatgc tcgccctgga ccgcgctgtg tgcagtcttc actcgaaaac ggaaagacga
      121 ctgtcgcaat tgatcgatga agtcggcgtg agcggcggag ccgcgactgg tcaccaaacc
      181 gatttggagg gggaccatcg gtatcggaag cagagcgttg gcgcgcaagg aaccgtccac
      241 cgtcatccgg cgaatcacgt cggcacggtc gagggcgatg cgacctgcgg tgaatgccgg
      301 atccacctcg agcagggata gccggactcg agacccccgc tcatcgacgc caatcgtgcc
      361 attcacccgc gcttcgatgc cgtcgcggaa cgagccgagc cccgattcgg tcaggaggcg
      421 atcgacctcc atcatgaccc ggccccgggc cgcaacctcc agggcgttct cggacgcgtc
      481 gctgtcggcg aggcggaaga acaccgctcc acggctggtc cggcgcaaac ccgtgacttc
      541 acctcgaacc cagatcggtg tcggaaacgc tcgaaccacc gcctcctgga ggcctttgag
      601 gacctgggac accgtgaagg tctggtcggt catctcgacg cctcgtaggt ggattggaca
      661 tcgctgagga tcagatgatg atcgaaggcg accatgctcg ggtcggcgag gacatcgtcc
      721 agatcccaga ttcgggcgtc ggccgcgtca tcgcctgatg tcggctggcc cagatcggct
      781 acgtgagccc agaagacgac ggtcaccacc ctcattctcg ggtcacgacc cggagctccg
      841 taggcaccta gttgggtcag cttcccgggt tgcagacccg tttcctcacc gagctctcga
      901 atggcggcag gctccaggtc ctcgtcgatg tcaacgaaac caccgggcaa cgcccagcgg
      961 ccccggtagg ggtcatggga acgttgcacg agcacgaccc cgagccgtcc ctccacggtt
     1021 gtcagcaagg cgacatcggc ggtcactgcg aacgggggga atctggatgc gtcgtatccg
     1081 ggcggagcac tcatcgtgat cgacgatagg gcggatctct cgtttcgagg ttggccaggc
     1141 atgggcgccc agcccgccta tccgggtcgt agcggccggg agcccatatt ctgcgcccac
     1201 atgggcaacc aagccgacga agccggtcct ccaagcctgg agcctgccgc ttcgaccgaa
     1261 tcgcgattcg atcgattctc ggctctcgtc gctctcactc tgttcgggct gacctggcca
     1321 gtgctcgatc tgctcgggcg taacgccgag ttcttcctgg cgcgccggtc ggcgaaagca
     1381 gagatcctgt tcgtagcgct gacggcgatc gtcgctgtcc cggtggcagt cggcgccctc
     1441 ggattccttc cgggtcgtgc cggccgagtg atcgcccttg gcctcgtcgc ggtcacggcg
     1501 agctccctcg ccctgctcta cctggcccga accgaactgc cctggtggct gtcgacgagt
     1561 gtcgcccttg gaatcggtct cggcacgacc tgggcattcg tgcacgtgac gccggtccgc
     1621 caggccgtcc gctatttggt gattgctccc ctggtcatgc tcggcgtgtt tctcttctcc
     1681 atgccggtcg gcgccgtgct gtgggagccg gacgtcgcgt tgggcaaccc ggttccgatc
     1741 gaaagcccgg ttccgatcgt gctggtcgtc ttcgatgagt tccccgttgc ctctctgatc
     1801 gaccccgaag gcgatctcga ggcggatcga ttccccaact tcggtcgtct tgcctccgat
     1861 ggcacctggt accgcaacgc cgtcacggtg gaacagcaga ccgagcagtc ggtgccggcg
     1921 atcctcaccg gtcgaatccc cggagattcg ttggtgccgg tggccgggca atacccgttc
     1981 aatctcttca ccgccctgcg cagcgtctac gacctccacg ctcacgagtc gatcacacaa
     2041 ctgtgcccgc gccagctctg cgagggcatt tctgcaaccg cttcttcgct ggtgggcgac
     2101 gtgtcagtgg tcgccggcca tgtcctgttg cccgagccgc tcagcgagga ccttccccct
     2161 atcgatcgcg ggtggggtga cttcgccaca gcggccggga ccttcaacgc ccgacaggag
     2221 tttcgcgagg tgctgagccg ggggccacgg gcagccatcg acgccactct cggcgacatc
     2281 gaagctgctg acgtttcccg gcccacgttg ttctacatgc acgccatcgt gccccaccac
     2341 ccatggcaat acctgccgga cggtcgcagc tatccgttca tcgtcgccac gaacccggcc
     2401 agccggggag ggggctggaa cgacgacgag ttcctggtgg cccagtcgat gcagaggcat
     2461 ctgctccagg tcggctatgc cgaccacgtg ctcggcgagg tgatcgcagc cttggagaag
     2521 agaggcctct atgaggatgc aatggtgatc gtggtcgccg atcacggcat caccatcaga
     2581 cccggggtgg atcatcagcg gatgatcaca gaagaaacca tcggctccat agccgcggtg
     2641 cccctgttcg tgaagacgcc caacggtgac ggcggcgtta tcgacgatcg gcgcgctctc
     2701 acgatcgaca tactccccac gatcgcggac gtcatcggtg cgagcctgcc ctcggacgtg
     2761 gaggggcttt cgttgctgca gcctccaccc gatcggaccc tgaccaccac ctatgggccc
     2821 gaagggcagg tgacgttcgg ggtatcgggg gaggagaagc tcgaagtagc gagagccatc
     2881 ttcgagcgct tcccgaatgg cgacccctgg gcccttcgcc cgcttggcgc tcccgacctt
     2941 gcgggctcgg agatcgatcg ggattcactg tcctcgtccc ggttgcgtgt catcgtcagg
     3001 gagccaggtc tttatcgcag cgtggacacc gaaggtgcag tgattccggt ccgtatcgga
     3061 gggaccctcc tgggtgaggc tgagggcgac gaggttctcg cggtggcggt caacggggtc
     3121 atcgcagcgg tcacccgtgc ctacgaaggc gatgacgcgg ttgcgtttct cgccatggtg
     3181 ccgccggagc gtttccggga cggcgagaac gtcatcgacg tcctcgaggt caccaacgat
     3241 ggtcggctgc ttcgtctgga cagttgacct cgccgactct gtctgggtga gtgatcagag
     3301 acgaccgatc agctcgacga ggatgtcgta gaagcggtcg gcgtcgatct cgttcatgac
     3361 gagggcgttg ggctcggacc tggtgacgcc ccaccagtcg acgagagtca tgccctgagt
     3421 ggggccggga gcggtctcga tctccaccgc cacctccttg ccggcgaaga tgtcgggcga
     3481 caggagatag acgatgacgg tggggtcatg gagagggccc ccttccatcc cgtacttctc
     3541 gatgtcgtga cggtcgtaga acctgagcat ccccgccaca gagctgcccg ccgtagtccc
     3601 gatctctgtg aagcggcgca gacggtccgg ggtggtgagt gccttgtgcg tcacatcgag
     3661 gggcatcatc accagcggga ccccgctggt gaacacgaca tgggcagcgt gggggtcgac
     3721 gaagatgttg aactcggccg cgggtgtcgt gttgccgccc tcgaagaaac tgccacccat
     3781 caggactatc tgatcgatct tccccgcgat gtcgggctcc ttcaccagag caagggcgac
     3841 attggtcaaa gggcccaacg tgcacacggt tgtcggctct gaccgaccca tgaccagttc
     3901 gacgagtcga tccacggcgt gtccggatga gatctccagc ctcggaggcg gcaggtcggc
     3961 tccgtcgagc ccggtcggcc cgtggacata ctcagcggtc accagccggt tgaccatcgg
     4021 ccggacagct ccctggtaga cgggcacttc ggagcggccg gccaactcga cgagggcaag
     4081 ggcgttgttg gtcaccttct cgatcggcac gttcccggcg acggtggtga tggccagcac
     4141 gtcgagctct tccggcgatg cgagagcggc gagaatggcg actgcgtcgt cctggccggg
     4201 atcggcgtcg atgatgattg cgcgcttgat gccggcaacg tagtcagtca ctcagcaatc
     4261 tctccacttg ctgcgacgtc ggcatcgatg attgcgcccc tcgtgtcgtc gttgccaggc
     4321 tgccggccgc tgtggcgcgc tccacagcca cctcgagaga caagtcacgg cccagagcat
     4381 cggcgagggc tccgcagaag gcatcgccgg ctccggtcgg gtcgacggcc tccacttcgg
     4441 gcgcgggaaa tcgatcagct tcgccgtccc acagcagcag ggccccatcc gagccgagtg
     4501 tcaccacgat cgcggcatcg atgcggagct tccgtgccgc cgcaatcacc tcgtcgatgg
     4561 aaacgggctc ggtgacaccc gccagcactc ccagttcgcc tcggttgggg atgagaacat
     4621 cggtccgggc cagaacctcc gaggggagct cggtggcagg tgccgggttg aggcagaaga
     4681 tcccgtcgac cagccgagct gcggccgcga ccgcgtcgat cggtacctcc agctgggcga
     4741 gaacgactcc gccggctgcg atttgatcgt gctgagcctc gatgtgctca ggggtcaatc
     4801 tggcatttgc gcccgggctg acgacgatcg tgttctcggc ggcatcgtcg atggtgatca
     4861 cagccaaccc tgttgcggcc cctctgtcga agcccactcc ttcgacgtcc accccctcgg
     4921 cggcaagggc tttccgaagc ccgtggccat ggaggtcgtc tccgacacgg ccgaccatcg
     4981 ccacagatcc ccccatccgg gccaccgcca ccgcctgatt ggctcctttg cccccgttgt
     5041 cccagtaatg ccccctcccg agcacggtct cgccgggccg ggggtgcgac gatgtgatga
     5101 cggtgagatc gtgattcagc gaccccacta cgacgatgtc agccattcga agagactaag
     5161 tcggtcgagg gaaggccatc gaggcggtgc acgagggcag gtaaaggggg tgacgcggct
     5221 gacccgatgc ggtcaatccg agacaccggg gattgacacc tgagaggagc gcggcgacct
     5281 cggacgaccg cggtgttcgc gtcttcggat tgagcttcag accgtgatta ccccatgcga
     5341 ccacgaccag atccgccgaa ccgatggcgc gttcgatcgc agagtcgttc tcggggccga
     5401 ccgggtcggg atgtcgggcg agtgtttcgg gagagacact gcgcagggca aacagattga
     5461 ccaccagggc tgatccgaaa ccccagagcc tcgagaaggc gactacccgt ctgatcgtcg
     5521 ggtcatccac tgtctccgtg gcggtggagg ggttgagcat gacccaggac actggtggtt
     5581 gcccgacatc ccagacccga gtcagccagt agcggtaacc cgtcggcatc gatcgaagga
     5641 tatgggtcat ccttacactc ggggtcatgg agaccttcga tgcgatacgc ac
//
LOCUS       DASUTA010000037         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_14118, whole genome shotgun sequence.
ACCESSION   DASUTA010000037 DASUTA010000000
VERSION     DASUTA010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14118"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VFZ80_00755"
     CDS             complement(<1..552)
                     /locus_tag="VFZ80_00755"
                     /inference="COORDINATES: protein motif:HMM:NF017951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF993 family protein"
                     /protein_id="HEX6285989.1"
                     /translation="MLSLPTGDGELIAWRPSGTPPIEPELGGFHSRVAFAATHVIPDP
                     LRERQPGASPIIDWDATLSIRRHLWELGLGVAEAMDTAQRGSGVAWDTARDLIRRTAT
                     EAEGPAVYGAGTDQLAAGGEYSVDEITEAYREQVSFIEELGGQAIVMASRHLAAAARS
                     PEEYVAVYDEVIASSSRPLLIHWL"
     gene            complement(569..1585)
                     /locus_tag="VFZ80_00760"
     CDS             complement(569..1585)
                     /locus_tag="VFZ80_00760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6285990.1"
                     /translation="MSNADRFGVFLPSFIWSDDGPERARGIVDFSRHAEDLGLGSIFI
                     TDHLLTARRLYSVSFLEPLTTLALVAGVTERIMLGTSVMVVPIREPVLFAKQVANLHF
                     LSRGRFILGAGIGWYPKEYEAVGISKSERGARTDEILDIMIPLLEGKSVTHHGQFYSV
                     DDVEIEPSADTRPELWIGGGSQLADPASPEAPRFATAVKRRILRSEGWIPRPTCPPDD
                     IRRDWDELQDYFAENGRDPTTITIAHENFMHFVPTQDRERALQEQHETFLRVMSDERG
                     PKYLEQVYLFGTPDDIVESLQSRVDAGVEQFMIHTLTPDPAQLEQWVEHIIPHVRFPE
                     EGSE"
     gene            1749..>2161
                     /locus_tag="VFZ80_00765"
     CDS             1749..>2161
                     /locus_tag="VFZ80_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6285991.1"
                     /translation="MKSWKKLVAILSVLALVLAACGDDGGTDTTTDGGTDTTADGGTD
                     TTAGEEPGTTAAGGGDPIVAATSLPLTGEFSIPAELHRNGYQLCVDLINERGGVLGRE
                     LVLEVEDNQSDQEIAVTQYERMVAEADILFCTFSSL"
BASE COUNT          402 a          633 c          714 g          412 t
ORIGIN      
        1 cagccagtgg atgaggaggg ggcggctcga cgacgcgatc acctcgtcgt agaccgcgac
       61 gtactcctca ggggagcggg ccgccgctgc gaggtgacgt gacgccatta cgatggcttg
      121 tccccccagc tcctcgatga agctcacctg ctcccgatat gcctcggtga tctcatccac
      181 tgagtattcg cctcctgcgg cgagttggtc ggtgccggct ccgtatacgg cgggtccctc
      241 ggcttcggtg gcagtgcggc ggatcagatc gcgcgccgtg tcccatgcga cgcccgaacc
      301 gcgttgggcg gtgtccatcg cctcggccac tccgaggccc agctcccaca gatggcggcg
      361 gatggagagc gtggcatccc aatcgatgat gggcgatgct cctggttgtc gttcccggag
      421 tgggtcgggg atgacatggg tcgcggcgaa ggcgactcga gagtgaaatc ctccgagctc
      481 gggctcgatc ggaggtgtgc ccgatggccg ccaggcgatg agctcgccgt cgccggtcgg
      541 caacgacagc atcacatccc cattccggtt attcgcttcc ctcttcgggg aagcgcacgt
      601 gcgggattat gtgctctacc cactgttcga gctgagcagg atccggggtc agcgtgtgga
      661 tcatgaactg ctcgactccg gcgtcgacgc gagactggag cgactcgacg atgtcatccg
      721 gcgtcccgaa gagatagacc tgttccaggt acttcggccc acgctcatcg ctcatcactc
      781 tgaggaatgt ctcgtgttgc tcctgaagtg cccgctcccg gtcttgagtg ggcacgaagt
      841 gcatgaagtt ctcatgtgcg atcgtgatcg tggtcgggtc ccggccattc tccgcgaagt
      901 agtcttggag ctcgtcccaa tctcgccgga tgtcatcggg cggacaggtc gggcggggaa
      961 tccaaccctc tgaacggaga atccggcgct tgacggcggt ggcgaaccgc ggtgcttcgg
     1021 gagatgccgg gtcggcgagc tgggaaccgc cacctatcca caactctggg cgtgtgtctg
     1081 cgctcggctc tatctcgaca tcgtcgaccg agtagaactg cccgtggtga gtgacggact
     1141 tgccttcgag gagcgggatc atgatgtcga ggatctcatc ggtgcgcgcc ccgcgctctg
     1201 acttcgatat accaacggct tcgtactcct tcgggtacca cccgatgccg gctcccaaga
     1261 tgaaccgacc cctggagaga aaatggagat tggcgacctg cttggcgaag agcaccggct
     1321 ctcgaatggg aaccaccatc accgacgtgc ccagcatgat ccgctcggtc acacctgcga
     1381 ctagcgccag ggtggtcagc ggctcgagga aggaaaccga gtagagacgt cgggcggtga
     1441 ggagatggtc cgtgatgaat atcgagccga ggccgagatc ttcggcgtgt ctcgagaagt
     1501 cgacgatgcc acgagcccgt tccggaccgt cgtcgctcca gatgaacgat ggaaggaaca
     1561 caccaaaacg gtctgcgttg ctcaaatatg gtgcccttct ggctgcaatc gattgattaa
     1621 gacgatagcc tcgctcggga atgtgagcca cgcgacatgc tggcttgcct tggagagacg
     1681 atcgtacaat cgattgcagc gagacgtgcg ggagtagccg tggcgtgcgg atgtagagga
     1741 ggagcagtat gaagtcgtgg aagaaactgg tcgccatcct cagcgtgctg gcgctggtgt
     1801 tggcagcatg cggcgacgat ggcggtacgg acacaaccac tgatggcggg accgacacca
     1861 ctgccgatgg cgggaccgac accaccgccg gggaggagcc gggtaccacc gcggctggtg
     1921 gcggagaccc gatcgtcgcg gcgacctcgc tgcctctcac cggagagttc tccatcccgg
     1981 cggaattgca ccgaaacggc tatcagctct gcgtcgatct catcaatgag agaggcggtg
     2041 tgttgggtag ggagctcgtg ttggaggtcg aggacaacca gtccgatcaa gagatcgcag
     2101 tgacccagta cgagcgcatg gtggcagagg cggacatctt gttctgcact ttctccagtc
     2161 t
//
LOCUS       DASUTA010000038         2583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1439167, whole genome shotgun sequence.
ACCESSION   DASUTA010000038 DASUTA010000000
VERSION     DASUTA010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439167"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1820)
                     /gene="groL"
                     /locus_tag="VFZ80_00770"
     CDS             complement(189..1820)
                     /gene="groL"
                     /locus_tag="VFZ80_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851179.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX6285992.1"
                     /translation="MAAKDLRFSEEARKGLQAGVDKLADVVKVTLGPKGRNVVLEKKW
                     GAPTITNDGVTIAKEIELEDAWENMGAKLAYEVANKTDNVAGDGTTTATVLAQAMVKE
                     GLRQVAAGANPMELKRGIEAAVEAAVKAIGDMARDIETTEEIAHVASISANNDNSIGE
                     VIADAMDRVGKDGVITVEEGQTFGLELEFTEGMQFDKGYISPYFITDPDRQEAVLDDP
                     YILIANQKVTSVNDLVPVLEKVMQTGKGVLVIAEDVEGEALATLVVNKIRGTFQSIAV
                     KAPGFGERRKAMLQDIAILTGGTVISEEVGLKLENVSTELLGRARKVVIAKDNTTIID
                     GAGSEADVKARIVQIEREIENTDSDWDREKLQERLAKLSGGVVVIKAGAATEVELKEK
                     KHRIEDAVQAARAAVEEGVVPGGGVALLRAQSAVEKVRGGSDDEKTGRNIVRKSMEEP
                     LRQIAVNAGFEGGVVVERVRAAEGSNGFNAATGVYEDLLAAGVIDPAKVTRSTLQNAA
                     SIAALLLTTEALVVEQPEDAPAMAGGHGHGGDMDF"
     gene            complement(1835..2125)
                     /gene="groES"
                     /locus_tag="VFZ80_00775"
     CDS             complement(1835..2125)
                     /gene="groES"
                     /locus_tag="VFZ80_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX6285993.1"
                     /translation="MKLQPLGDRVVVTPTEDAEARTPSGLVIPDTAKEKPQVGEVIAV
                     GPGARDDDGERIAMDVSVGDKVLYSKFAGTEVKLDSGEYLVLSERDILAIVN"
     gene            complement(2310..>2583)
                     /locus_tag="VFZ80_00780"
     CDS             complement(2310..>2583)
                     /locus_tag="VFZ80_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX6285994.1"
                     /translation="AIVDVLVDKTFAAVDATGARTVAGGGGVLANRRLRTRFAEEADK
                     RGVTLALPDPILCTDNAAMIGVAGAYWLSQGRITDWAENVDPSMVL"
BASE COUNT          449 a          844 c          762 g          528 t
ORIGIN      
        1 gcgcattgcg ttctcgccca caatcagtct cccaacagca cgcaatggtc gtatggctgt
       61 accgcaacga tacaacgaac cggacccgaa tgaccaaggg tcgatcgact catcgtcgca
      121 gccaggagac aatgaggaaa ggggcccgaa tcgggcccct ctctctctcc agtatttgtc
      181 cgtcggacct agaagtccat gtcaccgccg tggccatggc caccggccat ggcaggcgca
      241 tcctccggct gctcgacgac caatgcctcg gtcgtcagaa gcaaggccgc gatcgaagca
      301 gcgttctgca gcgtcgaccg ggtcactttg gccgggtcga tgaccccggc agccaacaga
      361 tcctcgtaca cgcctgtggc ggcgttgaac ccattgctgc cttcggcggc gcgaacccgc
      421 tcgacgacga ctccgccctc gaaaccggcg ttgaccgcga tctgacggag tggctcctcc
      481 atcgacttcc ggacgatgtt gcgaccggtc ttctcgtcgt cggatccgcc acggaccttc
      541 tcgaccgcag actgggcacg aaggagggca acaccaccac cggggaccac gccctcttcg
      601 acggcggcac gtgccgcctg aacggcgtcc tcgatgcggt gcttcttctc cttgagctcg
      661 acctcggtgg cggctcccgc cttgatgacg accacaccgc cggagagctt ggcgagacgc
      721 tcctggagct tctcgcggtc ccaatcagaa tcggtgttct cgatctcacg ctcgatctgg
      781 acgatgcgag ccttgacatc ggcctcgcta ccggcaccat cgatgatcgt ggtgttgtcc
      841 ttggcgatga ccaccttgcg ggcccgaccc agcagctcag tgctgacgtt ctccagcttg
      901 agaccgacct cttcggagat cacggtgcca ccggtgagga tggcgatgtc ctgcagcatg
      961 gccttgcgac gctcgccgaa gccaggggcc ttgacggcga tcgactggaa ggtgccgcgg
     1021 atcttgttca caacgagagt ggcgagggct tcgccctcca catcctcggc aatgaccaga
     1081 acaccctttc cggtctgcat gaccttctcc aacacaggaa ccaggtcgtt gaccgaggtg
     1141 accttctggt ttgcgatcag gatgtacggg tcgtcgagaa ctgcctcctg acgatccgga
     1201 tcggtgatga aataggggga gatgtagccc ttgtcgaact gcattccctc cgtgaactcg
     1261 agctcgagac cgaaggtctg tccctcttcg acggtgatca cgccgtcctt gccaacacga
     1321 tccatggcgt cggcgatgac ctcaccaatc gagttgtcgt tgttcgccga gatcgatgcc
     1381 acatgggcga tctcttcggt ggtctcgatg tcacgagcca tgtcaccgat cgctttgacc
     1441 gcggcctcga cggcggcctc gatgccgcgc ttgagttcca tcgggttggc gcctgcggcc
     1501 acctgacgca gaccctcctt gaccattgcc tgggcaagga cggttgccgt ggtagtcccg
     1561 tcaccggcga cgttgtcggt cttgttggcg acctcgtatg cgagcttggc gcccatgttc
     1621 tcccaggcgt cctcgagctc gatctccttg gcgatggtca caccgtcgtt ggtgatggtg
     1681 ggggcccccc acttcttctc caacacgacg ttgcggccct tggggccaag ggtcaccttg
     1741 acgacatcgg cgagtttgtc tacacccgcc tgaagccctt tccgggcctc ttcactaaag
     1801 cgaagatcct tcgctgccat aacttctcag ctcctcaatt gacgatggcc agaatgtcgc
     1861 gctcgctgag gaccaggtac tcgccggagt cgagcttgac ctcggtgccg gcgaatttcg
     1921 agtagaggac cttgtcccct accgatacat ccatggcgat gcgctcgccg tcgtcgtccc
     1981 gggctccggg accaacagcg atgacttccc ctacttgtgg cttctccttg gcggtgtccg
     2041 ggatgaccag acccgagggg gtgcgggctt ccgcatcctc cgtgggcgtg accaccactc
     2101 ggtcgccgag cggttgaagc ttcatacgta gctcctcctt gagctctacg tcgtgcttac
     2161 ttgcgggagc ctggcaccgg cgaatgaact accggaaacc ggcggctgac ccgatcgtta
     2221 gcactctctg attacgagtg ctagatgaat gtagcactct cgggccgcga gtgctaaatc
     2281 gaatctgagt cttgggcgct taggttctat tacagaacca tggaggggtc gacgttctcc
     2341 gcccagtcgg tgattcgccc ctgcgaaagc cagtaggccc cggcgacgcc gatcatggcg
     2401 gcattgtcgg tgcagagaat ggggtcgggc aaggcgagcg tcacaccgcg cttgtccgcc
     2461 tcctcggcga atctcgtccg gagacgtctg ttggctagga cgccgccacc accggcgacc
     2521 gtccgggccc cggtggcgtc gacggctgca aaagtcttgt ccacgagaac gtcgactatc
     2581 gcc
//
LOCUS       DASUTA010000039         7155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1050719, whole genome shotgun sequence.
ACCESSION   DASUTA010000039 DASUTA010000000
VERSION     DASUTA010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050719"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2192
                     /gene="secA"
                     /locus_tag="VFZ80_00785"
     CDS             <1..2192
                     /gene="secA"
                     /locus_tag="VFZ80_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX6285995.1"
                     /translation="HIHRFLGLSVGLIQNNMPTADRHPAYRSDITYGTNNEFGFDYLR
                     DNMAMSAENRVQRGHVYAIVDEVDSILIDEARTPLIISGKLSDSAKWYRDFAKIARRL
                     QPDLHYEVDEKKRQVLVTEEGVARVEQILGVENLYDHAAVDFVHHLETALKAETLYHN
                     DVEYIIDHGEVKIVDEFTGRVLEGRRYSEGLHQAIEAKEGVAIKEENQTLATITLQNY
                     FRLYEKLAGMTGTAETEAAELASIYNLEVATIPTNEPIARMDEGDLVYKTEAGKFDAL
                     IRDVKERNERGQPVLMGTVSIEKSELLSKALDKVGVKHEVLNAKHHAREAEIIAQAGR
                     PGAVTVATNMAGRGVDIRLGGNPEEMAKFELKRRGVDPSDPSYEELEKKLTEEYEIQI
                     KPDKKAVLDAGGLYVVGTERHESRRIDNQLRGRSGRQGDPGESRFYLSLEDDLMRRFQ
                     GEWVSNIMERLRMPEDQPIEAKMVSKSIERAQRQVESQNFEIRKNVLKYDEVMNTQRE
                     SIYDWRKSILEGTASEDLIDDWIEDALSDIVESHFGGLAKSEWDWDALKLELTQIYPT
                     KVDASKFSGSYDVENVIDFVVDEALTRYQEREAELGKEILEKVEKSVMLSVIDNKWRE
                     HLSEMDYLRAGIGLRAMGQRDPLSEYQREGYDMFVGTVESVKRDAVRYLFNVEVAQPK
                     TQPQRVEANPTGGKGPAKPATSEKIGRNDPCPCGSGKKYKKCHGAVA"
     gene            complement(2214..3101)
                     /locus_tag="VFZ80_00790"
     CDS             complement(2214..3101)
                     /locus_tag="VFZ80_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6285996.1"
                     /translation="MEMRPKAFGENLNEWFRGLGQTWKPMLVLCAIVFVPLGILTTAL
                     ILIPGALDSYFDLIDPNLDLQTPAELLELLGPLLWVGAIWAVLQLVATVLVYIAAGRV
                     IAVRSVGSEATARELLRFAGRRLGTGLGAGLMLALGLLAILAAAVLVGWAVLSGGGAN
                     FLTIFLTAVVALTALVICVWLGVGVSLYPQAIAMDAVNAPRSLVASFRLVKTRWWPTL
                     GFELVAGLIVSAVAQVLSFVLVPILFLGMVAPVFLAVGYGLATMLQGPVAASIGLAYA
                     VWYVDLRAREQPMRGEDLV"
     gene            3178..4299
                     /gene="prfB"
                     /locus_tag="VFZ80_00795"
     CDS             3178..4299
                     /gene="prfB"
                     /locus_tag="VFZ80_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_148128604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX6285997.1"
                     /translation="MNDPTAFAAELRQRLEEARGFLDLEGKAADLDELREKAASPDLW
                     DDPDEARVISQRLARYEALFDKVEGLATQIEDAEVLLELSEEANDPDSRAEAIELLKG
                     ASGSLDELELESLFFDEYDEADAILSVHAGAGGVDAQDWAEMLARMYQRFLSDANFAV
                     TVEEATEGDEAGIKSITMTVKGDRAYGTLESERGVHRLVRISPFDANSRRHTSFAGVD
                     VVPDLGDQADVEINEEDLRIDTYRSQGAGGQHVNTTDSAVRITHIPTGVVVQCQNERS
                     QLQNKARAMAMLKARLAERARLERAEHLAEIRGEKGEAAWGRQVRSYVLQPYQMVKDL
                     RTDYEVGNVQGVLDGDLQGFVEAYLRWRRSEFELGDNSG"
     gene            4375..5067
                     /gene="ftsE"
                     /locus_tag="VFZ80_00800"
     CDS             4375..5067
                     /gene="ftsE"
                     /locus_tag="VFZ80_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX6285998.1"
                     /translation="MIRLDNVTKVYEGGTVAANDVSLDIQKGEFVFLVGASGSGKSTL
                     IRLMLRDEPVTRGKIWVAGKDITTLPRWKIPYLRRSVGTVFQDYKLLPNKTVRENVAF
                     ALEVLGRPQSVIGPQVDQVLELVGLDDKGARYPRQLSGGEQQRVSVARSFVNRPPIML
                     CDEPTGNLDPKTSVGIMKLLDRINRTGTTIVMATHDHAIVDAMKRRVVALEHGKVTRD
                     DETGGYEGVREA"
     gene            5064..5987
                     /locus_tag="VFZ80_00805"
     CDS             5064..5987
                     /locus_tag="VFZ80_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX6285999.1"
                     /translation="MSRVIFLLKEAMVSLRRNVLIVAGAILAVFISLSLALGALVVNE
                     ILRINTIAWQQGVHVIAFLNDEGATGVPVGTHDQLLQEVRGWEEVQDAFYLSKSEAWV
                     EYQEIFAGQDELLDINPAILPASIRIELVDIELHSSVRFRLEQQAGSVRQVATAAESI
                     EQLQALSNVLNVLGLGMAIVLGVAAVVLIANTIRLAIYARRDEVAIMKLVGASNWFIR
                     VPFLLEGMIEGLVGAALAVFAVWLGATNLAQAGETFALFRLDVSQQFFFRWGLLFLIF
                     GAVAGIVGSMLGLSKYLRDADGTGPDVPTGF"
     gene            5999..6469
                     /gene="smpB"
                     /locus_tag="VFZ80_00810"
     CDS             5999..6469
                     /gene="smpB"
                     /locus_tag="VFZ80_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX6286000.1"
                     /translation="MSSEDSGRVKVVATNRNARRNYTIIDTYEAGLALVGSEVKSLRA
                     GKMELKDSYGDIRGGEAYLVGAHIGPYDYAHQGGHDPERLRKLLLHRREIDRLAGGLA
                     EKGLTLVPLRVYFKDGNAKVELGLARGKTTVDKRQTLRDREHQREIERAVRRRR"
     gene            6551..6900
                     /gene="ssrA"
                     /locus_tag="VFZ80_00815"
     tmRNA           6551..6900
                     /gene="ssrA"
                     /locus_tag="VFZ80_00815"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1517 a         2176 c         2245 g         1217 t
ORIGIN      
        1 gccacattca cagatttctc gggctgagtg tcggtctgat ccagaacaac atgccgacgg
       61 ccgaccgaca ccccgcatat cggtcagaca tcacctatgg gaccaacaac gagttcggct
      121 tcgactatct gcgcgacaac atggcgatgt cggcggaaaa cagggtccag cgtgggcatg
      181 tctacgccat cgtcgacgag gtggactcga tcttgatcga cgaagccaga acccctctga
      241 tcatctccgg gaagctctcc gactcggcca agtggtatcg ggacttcgcc aagatcgccc
      301 gccgcctcca gccggacctc cactatgagg tcgacgagaa gaagaggcag gtgctggtaa
      361 ccgaagaagg agtggcccgg gtcgagcaga tcctgggtgt ggagaacctc tacgaccacg
      421 ctgccgttga cttcgtccac catctcgaga cggctctcaa ggccgagacc ctctatcaca
      481 acgacgtgga atacatcatc gaccatggcg aggtgaagat cgtcgatgag ttcaccggcc
      541 gtgtcctcga aggtcggcgg tactccgagg ggctacatca ggcgatcgag gccaaagaag
      601 gcgtggcgat caaagaagag aaccagacac tcgccaccat cacgctccag aactacttcc
      661 gtctctacga aaagctggcc ggcatgaccg gtaccgccga gacagaggcc gccgagctcg
      721 cctcgatcta caacctcgag gtggcgacca tccccaccaa cgagcccata gcccgcatgg
      781 acgagggcga tctcgtctac aagaccgagg ccggcaagtt cgacgcgctc atcagagatg
      841 tgaaggagcg caacgagcgg ggtcagccgg tcctcatggg cactgtgtcc atcgagaagt
      901 ccgagctgct ctccaaggcg ctggacaagg tcggtgtcaa acacgaggtc ctcaatgcca
      961 agcatcacgc acgggaggcg gagatcatcg cacaggccgg ccggcccgga gcggtgactg
     1021 tcgccaccaa catggcaggt cgtggtgtcg acattcgcct tggcggcaac cccgaggaga
     1081 tggccaagtt cgagctgaag agacggggag tcgatccgtc cgacccatcc tacgaggagc
     1141 tcgagaagaa gctgaccgag gagtacgaga tccagatcaa accggacaag aaagcggttc
     1201 tcgacgccgg gggtctctac gtggtcggca ccgagagaca cgagtcgcgc cgaatcgaca
     1261 accagctgcg cggtcgatcc ggccgtcagg gtgatccggg ggagagccgc ttctatctgt
     1321 cactggagga cgacttgatg cgtcgtttcc agggtgagtg ggtgtccaac atcatggagc
     1381 ggcttcgcat gccggaggac cagccgatcg aggccaagat ggtcagcaag tcgatcgaac
     1441 gagcccaacg acaggtcgag tcgcagaact tcgagatcag aaagaacgtc ctcaagtacg
     1501 acgaggtgat gaacacccag cgggagagca tctacgactg gcgcaagtcg atcctcgagg
     1561 gcaccgcgtc cgaggatctc atcgacgatt ggatcgaaga tgctctcagc gacatcgtcg
     1621 agtcgcattt cggcggcctc gcgaagagcg aatgggactg ggacgctctg aagctcgagc
     1681 tgacccagat ctaccccacc aaagtcgacg cgtccaagtt ctcggggagt tatgacgtcg
     1741 agaacgtcat cgacttcgtc gtcgacgaag ctctcacccg ctatcaggag cgcgaggcgg
     1801 aactgggcaa ggagatcctg gagaaggtcg agaagtcggt gatgctctcg gtcatcgaca
     1861 acaaatggcg ggagcatctc tccgagatgg actacctccg cgccgggatc gggcttcggg
     1921 cgatgggcca gcgcgatccg ctctcggaat accagcgaga gggttacgac atgttcgtgg
     1981 gcacggtcga atccgtgaaa cgggatgcgg tcaggtacct cttcaacgtc gaggtcgctc
     2041 agcccaagac ccagccccaa cgcgtggagg ccaatcccac cggtgggaaa ggccctgcca
     2101 agccggccac gtccgagaag atcggtcgca acgatccctg cccatgcggg agcggcaaga
     2161 agtacaagaa gtgtcacggg gcggtggcct gaacaaaggc cacattcgcc gaatcagacc
     2221 aggtcttcgc cccgcatcgg ttgctcccgg gctcgcagat ccacatacca gaccgcatag
     2281 gcaagcccaa tgctcgcggc caccggccct tggagcatcg tcgccagtcc gtacccaaca
     2341 gccaggaaca ccggggcgac catccccagg aacaggatcg ggaccagaac gaagctgagc
     2401 acctgcgcga cggccgagac gatcagacct gccaccagct cgaatcccag tgtgggccac
     2461 caacgtgttt tcaccagtcg gaagctggcg acaagagagc ggggggcatt caccgcatcc
     2521 atggcgattg cctgtgggta gagagaaacc cccacaccca gccacacgca gatcacgagt
     2581 gcggtcagtg ccacgactgc cgtgagaaag atggtcagga agttggcccc gccccctgac
     2641 aggactgccc agccgacgag caccgcggca gccaggatgg cgaggagacc gagagccagc
     2701 atgagaccag cgcccaaccc ggtcccgagg cgccgtccgg cgaaccgcaa caactcccgt
     2761 gcggtcgctt cggagccaac ggatctgaca gcgatgacgc gtcctgcagc gatgtagacg
     2821 agcacagtgg cgacgagctg gagaaccgcc cagatcgctc ccacccaaag gagcggtccg
     2881 aggagttcca gcagctccgc cggagtctgc aggtcgagat tggggtcgat gaggtcgaag
     2941 taggagtcga gcgcacccgg gatcagtatg agggctgtgg tcagaatgcc gagggggacg
     3001 aagacgatgg cacagagaac cagcatcggt ttccatgtct gaccgagccc ccgaaaccac
     3061 tcgttcaggt tctcgccgaa tgccttgggc cgcatctcca cgctgccgga cggtagtgag
     3121 cggctcatcg cggggtctcg caatccagct gtgtgtcata agcttgaagc cttggagatg
     3181 aacgacccaa ccgcattcgc cgccgagctc cgccagcggc tcgaggaggc tcgaggcttt
     3241 ctcgatctgg aagggaaggc tgccgatctc gacgagctcc gggagaaggc cgcctcacct
     3301 gatctctggg acgatcccga tgaagcgcgt gtgatcagcc agcgtctggc cagatacgag
     3361 gcgctcttcg acaaggtaga gggtctcgcc acccagatcg aggacgctga agtcctcctc
     3421 gagctgtccg aggaggcgaa tgatcccgac tcccgtgccg aggcaatcga gctcttgaaa
     3481 ggcgcgtctg gatccctcga cgagctggag ctcgaatcgt tgttcttcga tgagtacgac
     3541 gaggcggacg ccatcctcag cgtccatgcc ggtgcagggg gagtcgacgc ccaggactgg
     3601 gccgagatgc ttgcccggat gtatcagcgc ttcttgagtg acgccaactt cgccgtgacc
     3661 gtcgaagagg caacagaagg ggacgaggcc ggcatcaagt cgatcaccat gactgtcaag
     3721 ggcgatcgcg cctatgggac gctggagtcg gaacggggag tgcaccgact cgtgaggatc
     3781 agcccattcg acgccaactc ccgccgccac acgagcttcg ccggcgtcga cgtcgtcccc
     3841 gatctcggag accaggccga cgtcgagatc aacgaagaag atcttcgaat agatacctac
     3901 cgttctcagg gtgcgggagg tcagcacgtc aacaccaccg actcggctgt ccgaatcact
     3961 cacatcccca ccggtgtcgt cgttcagtgc cagaacgagc gcagccagct acagaacaag
     4021 gcgcgtgcaa tggcgatgct caaagctcgt ctcgccgaaa gggcccgact ggagcgtgcg
     4081 gagcatctcg ccgagatccg gggtgagaaa ggtgaggccg cctggggtcg acaggtccgc
     4141 agctacgtcc tccagccgta tcaaatggtc aaggacctga gaacggatta tgaggtcggc
     4201 aacgtgcagg gggttctcga cggtgacctc cagggcttcg tggaggccta cctgcgttgg
     4261 cgaagatctg aattcgagct cggtgacaat tccggttaaa cgcgaaagcg gcgttaccgt
     4321 cacccattcc gcggcaataa ccccattagc tgcgagactt cctctcggtc ccccatgatc
     4381 agactcgaca acgtcaccaa ggtctacgag ggcggcaccg tcgccgccaa cgatgtctcg
     4441 ctcgacatcc aaaagggcga gttcgtcttc ctcgtcgggg cctccgggtc cggaaagtcg
     4501 accctcatcc gattgatgct gcgcgatgag cctgtcaccc ggggcaagat ctgggtggcg
     4561 ggcaaggaca taaccaccct gcctcgctgg aagatcccct atctccgtcg ctccgtcggg
     4621 actgtattcc aggactacaa gctgctcccc aacaagacgg ttcgggagaa tgttgcgttc
     4681 gctctcgagg ttctgggacg tccgcagtcg gtgatcggcc cacaagttga tcaggtcctc
     4741 gagctggtcg gtctggacga caagggggcg cggtaccccc gccagctctc gggtggcgaa
     4801 cagcagaggg tttccgttgc ccgctccttc gtcaatcgcc ctccgatcat gctctgcgac
     4861 gagcccaccg gcaacctcga cccgaagacc tcggtcggga tcatgaagct cctcgaccgg
     4921 atcaaccgga ccggcaccac catcgtgatg gccacccatg accatgccat tgtcgatgcc
     4981 atgaagcgac gggtcgtcgc cctggagcac gggaaggtga cccgcgacga cgagaccggc
     5041 ggatatgagg gggtacggga ggcatgagtc gggtgatctt tctcctcaag gaggcaatgg
     5101 tctcgctgcg ccgaaatgtc ttgatcgtcg ccggcgccat cctcgcagtc ttcatctcgc
     5161 tgagcctcgc tctcggagca ttggtggtca acgagatact ccgtatcaac acgatcgcct
     5221 ggcagcaagg tgtccacgtc atcgccttcc tcaatgacga gggcgccaca ggcgtgccgg
     5281 tgggcactca cgatcagctc ttgcaggaag tcagaggatg ggaggaggtt caggacgcct
     5341 tctacctgag caagtccgag gcctgggtcg aataccagga gatcttcgct ggacaagacg
     5401 agctcctcga catcaacccg gcgattctcc cggcctcgat ccgcatcgag ctcgtcgaca
     5461 tcgagttgca cagctctgtt cgtttccgac tcgagcaaca ggcgggatcg gtgagacagg
     5521 ttgccaccgc ggcggagtcg atagagcagc ttcaggcgct ttccaacgtg ctcaacgtcc
     5581 ttggcctggg catggcgatc gtgctcgggg tggcagccgt cgtgctgatc gccaacacca
     5641 tccgactcgc catctacgcc cggcgtgacg aggttgccat catgaaactg gttggcgcct
     5701 cgaactggtt catcagggtg ccctttctgt tggaggggat gatcgagggc ttggtgggag
     5761 ccgctctggc tgtgtttgcg gtctggctcg gtgccaccaa tctggcgcag gccggtgaga
     5821 cgtttgccct cttccgactc gacgtcagcc agcagttctt cttcagatgg ggtctgctct
     5881 tcctgatctt cggcgcagtg gccgggatcg tcggctcgat gctgggtctc agcaagtacc
     5941 tgcgggacgc cgacggcacc ggtcccgacg tccccaccgg gttctaggag gcgatgcaat
     6001 gagctcggag gattccgggc gggtcaaggt cgtcgccacc aatcgcaacg cccgtcgcaa
     6061 ctacacgatc atcgacacat acgaggccgg tctcgcgctc gtgggctcag aggtgaagtc
     6121 tcttcgggcg gggaagatgg agctcaagga ctcatacggg gacatccggg gaggtgaggc
     6181 atatctggtg ggtgcccaca tcggtcccta cgactacgcc caccagggtg gccatgaccc
     6241 agagcggttg cgcaagctcc tgttacaccg tcgcgagatc gatcggcttg ccgggggcct
     6301 agcggagaag ggtttgaccc tggtgccact acgcgtctac ttcaaagacg gcaatgccaa
     6361 agtcgagctc ggtctggcac ggggcaagac cactgtggac aaacgccaaa ctctgcgtga
     6421 ccgcgagcat caacgggaga tcgagcgggc ggttcggcgc cggagatagc aagcgtgtgc
     6481 aggaaccatg gccagaggac ttcaattgca cacgcttggg acgttagaat gtgatattcg
     6541 cccacgatat gggggtgaaa cggtttcgac gttgagtgtt gaggcgttgg aagcgagccg
     6601 gggtctccgg ggccccgttg acaaaccgga acaaccaata agtgccgaag acaacttcgc
     6661 actggctgcc taattaagta gcccgtcccc ctggctggtt cccgcccggt caggtaaggg
     6721 cgtcgcaatg cgggatgctc cgcccgggtt tccgggatcg ggcggctaag cacaagtccg
     6781 gataggcgca gagaaactcg tcgtcagagt cctgtagccg aagctcgctg acgactgcgc
     6841 tcggagatgc cagcgctgac ggctcagcgg accggggttc gactccccgc acctccacga
     6901 gagtcccgcg tcaatgcggg gctgcaggcc tgccatctgg caggcctgct ggttgtacac
     6961 atagctagcg acgagtcatg gaacacgcgc ccatcccaac cgaaagtcaa ctggagcgtg
     7021 ttcgagacgc ggtcggagcc tcctccctga ctcacagcca tcgtctcgaa ggcggtctca
     7081 gctgcacgat ggacgtccta tcggatggag cgacccgcat ggtgttgcgg agatacggtc
     7141 cttggtctca cggga
//
LOCUS       DASUTA010000040         2378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1568958, whole genome shotgun sequence.
ACCESSION   DASUTA010000040 DASUTA010000000
VERSION     DASUTA010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568958"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..>2378)
                     /locus_tag="VFZ80_00820"
     CDS             complement(13..>2378)
                     /locus_tag="VFZ80_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286001.1"
                     /translation="GLAPSPPGERAEHFAALRAFFQAIAARGTVLMVFEDLHWADEGL
                     LDFIVELVERTTADSILVMCLARPELLERRHDWAASRKRTLTMHLSRLDEESMRVLVA
                     GLAPGMSEAMVSRIADRAAGVPLHAVEFVRMLLNTGQLVKDGEEFRFTGDDEQIPIPD
                     SVSAIIGARIDRLEPGSQAILRDASVLGLTFSPSSLASLRGEDLGEIENFLGDLVRRE
                     VLEFDENPRSPERGQYRFVQGLIREVAYGRLSKQERVTRHLAVAEMFESADDPELAGV
                     VASHYADAVEADPGNHELMQKACRAVIEAAERAANLRSDAQAANLYERAAEMTTDKAQ
                     RSSLRLRAARCLARTGREDRGASIAREVLELAREQGDSAGQVRAVTALAHILSANFET
                     AEAADLTDAVYRTVEPSTDPDWIDLARETSRALFLSGRTEQSLAVANTAIPIMESQEA
                     VEEMLDTLINKAGALMFEGAALEPAAILLGVSEMAGERGLLPIKIRAINNLIAATQFD
                     KVSDIAANLELDALTQRVGDENWIVRGHFFAGQTLIDNGDLAGALQRVEAAEELDLSE
                     FWADSIEVVRLRAELYQLGYDEQRYRRHAEIQAKYGTSKDPQLQNGVLSANADMKLAI
                     GRVEEALEAALEIEAIPNSYPYAIQTAVSAAARLRDARTLTEILSVLEAEHSRGRASR
                     GLARVAQSHLLALNGDVDAAERAFIDAEELWAKTQGSLWLARARAIFALLAGTDHELA
                     VVKAEQARRFFVDNGYQLFLDGIMSELPDTEHWFDAGLLDRSVASSG"
BASE COUNT          418 a          786 c          717 g          457 t
ORIGIN      
        1 ggaggtcaga ccctagccac tcgacgcgac gcttcgatcg agcagaccgg cgtcgaacca
       61 atgctcggta tcgggtagct cgctcatgat tccgtcgagg aacaactgat agccgttatc
      121 gacgaagaag cgccgagcct gctctgcctt gacgaccgcg agctcatgat cggtaccggc
      181 gaggagggcg aagatggctc gtgccctagc caaccaaagg cttccctggg tcttcgccca
      241 cagctcttcg gcatcgatga aggcacgctc ggcagcatcg acgtccccgt tcagtgcgag
      301 cagatgggac tgggcgaccc gggcgagccc acgactggct cgccctctgg agtgctccgc
      361 ctccaacacg gacaggatct cggtcagcgt gcgtgcgtcg cgcaaccggg ccgccgccga
      421 caccgctgtt tggatggcgt atggatagct attggggatc gcctcgatct ccaaggctgc
      481 ttcgagtgct tcctcgactc ggccgatggc gagcttcatg tccgcgttgg cgctgaggac
      541 tccgttttga agctggggat ctttcgaagt gccgtatttg gcctggattt cggcatgcct
      601 ccggtacctc tgctcgtcgt atccgagctg gtagagctcc gctcgcaacc gaaccacctc
      661 gatgctgtcc gcccagaact ccgagagatc gagctcctca gccgcctcga cccgctggag
      721 ggccccggcc aggtctccgt tgtcgatgag agtctgtccc gcgaagaagt gaccacgcac
      781 tatccagttc tcgtccccaa ccctttgtgt cagcgcgtcg agctccagat tggcggcgat
      841 gtctgagacc ttgtcgaact gggtcgccgc gatgagattg ttgatggcac ggatcttgat
      901 ggggagcaac ccccgctcgc cggccatctc ggacactccc aggagtatcg ccgcgggctc
      961 caaggcagcg ccttcgaaca tcagcgcgcc tgctttgttg atgagggtgt cgagcatctc
     1021 ctcgaccgct tcctggctct ccatgatcgg tatggcggta ttggccacgg cgagggattg
     1081 ctcggttcgc ccgctgagga acagagcacg cgatgtctca cgggcgagat cgatccaatc
     1141 cgggtcagtc gaaggttcca ctgtccggta gacagcgtcg gtcaaatcgg cggcttcagc
     1201 cgtttcgaag ttggccgaga gaatgtgagc caatgcggtc accgctcgga cttgtccagc
     1261 ggagtctcct tgctcccggg ccaactccag aacttcccga gcgatcgaag ccccccgatc
     1321 ctctctgccc gtccgcgcca gacagcgggc ggctcgcagg cgtagcgaac ttctctgagc
     1381 cttgtcggtc gtcatttccg cggcccgttc gtagaggttc gccgcttgtg cgtccgacct
     1441 gaggttggcg gctctctcgg cggcctcgat aacggcacgg caagccttct gcatcagctc
     1501 gtggttcccc ggatccgcct cgaccgcatc ggcgtagtgg ctcgcaacta ctcccgccag
     1561 ctccgggtcg tcggccgact cgaacatctc ggcaacagcg agatgacggg tcaccctttc
     1621 ttgcttggac aggcgcccgt aggccacctc ccgtatcagg ccttggacga atcgatactg
     1681 gccacgctcg ggtgatctcg gattctcatc gaactcgagt acctccctcc tcacgagatc
     1741 acccaggaag ttctcgatct cgccgaggtc ctcgccgcgg agactggcca gagacgatgg
     1801 cgagaaggtc agacccagca cggaggcatc gcgaaggatc gcttgagagc caggctcgag
     1861 acgatcgatg cgggcaccga tgatcgcact gacgctgtct ggaatcggga tctgctcgtc
     1921 gtccccggtg aacctgaact cctcgccgtc cttcaccagc tggcccgtgt tgaggagcat
     1981 ccggacgaac tcgaccgcat gcagcggcac ccccgccgcc cgatctgcga ttcggctcac
     2041 cattgcctcc gacatgcccg gggccaggcc ggccacgaga actcgcatcg actcctcgtc
     2101 gagacgactg agatgcatcg tcaacgtccg tttgcgactc gccgcccaat catgtctccg
     2161 ttccaggagc tcgggcctgg ccagacacat gaccaggatc gaatctgcgg tcgttcgctc
     2221 gaccaattcc acgatgaagt cgagaaggcc ctcgtcggcc caatgcagat cctcgaagac
     2281 catgagaacg gtgccccgtg ccgcgatcgc ctggaagaag gcccgcaggg ccgcaaagtg
     2341 ctcggctcgt tccccgggtg ggctcggggc gagaccga
//
LOCUS       DASUTA010000041         4890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1568988, whole genome shotgun sequence.
ACCESSION   DASUTA010000041 DASUTA010000000
VERSION     DASUTA010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568988"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2220
                     /locus_tag="VFZ80_00825"
     CDS             <1..2220
                     /locus_tag="VFZ80_00825"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX6286002.1"
                     /translation="PWSNDPIADRPGEVLYVRDEESGEIWGATALPLRDDATSYLARH
                     GAGYSEFEHTSRGIALHLHQTVPLEDPIKISRLRLRNLTNRRRRLSVTAYVEWVLGTT
                     RAVSPMQIVTERDPTTGALFARNPWNVDFGDRVAFADLGAGEVVVTGDRAEFLGRHGS
                     VDDPAALRTAGGLSGAVGAGYDPCAAFQRSIVLAPGEEIDVVFLLGQGEDSDEARSLV
                     QRYRDTDPEQVRIEVARHWDDVLGTVRVKTPDRSFDLMVNQWLLYQTLACRMLGRTAF
                     YQSGGAYGFRDQLQDGMALVTPMRDSTREHLIRAAGHQFEEGDVLHWWHEPSGKGVRT
                     RISDDYLWLPYAVAHYVEVTGDIEILDQDVPYLRGPGLAPGQDEVFFTPEQSGTTGTI
                     FDHCRRALDRGLSLIGERGLPLIGTGDWNDGFNRVGREGRGESVWLGWFLDANLRRFA
                     EIAEGLGEAELASRWRQGAQRLRSSIEAEAWDGDWYRRAFFDDGTPIGSSMSPEARID
                     SIAQSWSVIHGGGIERRAGRAMESVEEYLVHRGDGLVLLFDPPFDEWEVDPGYIKGYL
                     PGVRENGGQYTHAAVWCAIAFATLGDGDRAGELFSLLNPINHASTRAGIHRYRVEPYV
                     AAADVYSKAPHVGRGGWTWYTGSAGWMYRAGVEWILGFRLRGSRLVIDPCIPRAWGEF
                     EIAFRYHSSRYEIRVSNPNAVSRGVVSLEMDGAVLPYGDGLDLLDDGSTHQVRVTLG"
     gene            complement(2365..4008)
                     /locus_tag="VFZ80_00830"
     CDS             complement(2365..4008)
                     /locus_tag="VFZ80_00830"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX6286003.1"
                     /translation="MTGPTAADLLVECLEAEGVGYVFGIPGEETLELNRALHASSIEF
                     VPVRHEQAGAYMANVVGRLTGRAGVCLGTLGPGATNLVTGVADAFLDRAPMVALTGQT
                     DQERMHKESHQHIDVVNLMDPITKWNARVGDPSIIPEVVRKAFKLAEAEKPGPTHIEL
                     PEDVMGEPYSGSPLRRQPPVRTEPSDADVSRAARVLDAARSPVALAGNGVVRNRAVTA
                     LRSFVRSVGLPTAVTFMGKGLIDPDDPLFLGAVGLQAGDFDMAGFDQADVVVAIGFDL
                     VELSPSLWNPSGDKTIICLDTLPAEIDQNFLPEVELVGDLNAVLDQLAAACTLIPHPG
                     GSTKLRQAVADRFDSAQKDEGYPMKPPRVLYDTRDALGRSDLLISDVGLHKLWVGRMF
                     PAHEPNTVLIANGLAGMGFALPAAIAAKLVQPERKVVTVCGDGGFLMNVQELETAVRL
                     STPFVTVIWEDRQFGSIALKQRRRFGSHFGVDFTNPDFVALAESFGLAAWRVTTANEF
                     GGLLREAITLPIPSLIVLEIDYDVEATITDELGEETVLT"
     gene            complement(4385..4801)
                     /locus_tag="VFZ80_00835"
     CDS             complement(4385..4801)
                     /locus_tag="VFZ80_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEX6286004.1"
                     /translation="MTEKTLYDRLGGVFAIAAVIDHFSDEVVKNPIVGQESQNPALRE
                     WHTNQLSRLPGLKFMRTLWVCNVAGGPFEYVATEPGLTALGLEEAHRKFRISSEEFDE
                     VAAELGRTLDFVGVPEKEKEEVLAAFAAHKSEVIVD"
BASE COUNT          840 a         1512 c         1612 g          926 t
ORIGIN      
        1 ccatggtcca acgacccgat cgccgatcgt cccggggagg tgctctatgt ccgggacgag
       61 gagagtgggg agatctgggg agcgactgcc ctgcccctgc gcgacgacgc cacgtcctac
      121 ctggcccgtc acggagccgg ctactccgaa ttcgagcaca cgtcgcgggg aatcgcgctt
      181 cacctccacc agaccgtgcc gctcgaggac ccgatcaaga tctcccggtt gcgacttcgc
      241 aacctcacga accgacgtcg gcggctctcg gtcaccgcct acgtggagtg ggtcctgggg
      301 acgacgaggg cggtctcgcc tatgcagatc gtcaccgaac gcgatccaac gacaggagcg
      361 ttgttcgcca gaaacccctg gaacgtcgat ttcggggacc gggtggcttt tgccgacctg
      421 ggggccggcg aggtcgtggt caccggtgat cgggccgagt tcctgggcag acatggcagc
      481 gtcgacgatc cggcggcgct gagaaccgcc ggagggctgt cgggggcggt cggcgcggga
      541 tatgacccct gcgctgcctt tcagcgttcc atcgtcctcg ccccgggcga ggagatcgat
      601 gtcgtgttcc tcctcggtca gggcgaggac agcgacgagg ctcgcagcct ggtccagcgt
      661 tatcgcgaca ccgaccctga gcaggtgaga atcgaggtgg cgagacattg ggacgacgtc
      721 ctcggcaccg ttcgggtgaa gacgccggac cgatcgttcg acctgatggt caaccaatgg
      781 ctgctgtacc agacccttgc gtgtcggatg ttggggcgga cggccttcta tcagtccggg
      841 ggggcatacg gattccgcga ccaactccag gatggcatgg cgttggtgac tccgatgcgg
      901 gactcgacga gagagcacct gatccgggcc gcgggccatc agttcgaaga gggcgatgtc
      961 ctccattggt ggcacgagcc ttcgggcaag ggtgtgcgca ctcggatctc cgatgactat
     1021 ctgtggctcc cctacgcagt tgctcactac gtcgaagtga ccggggacat cgagattctc
     1081 gaccaggacg ttccttatct gagaggacct gggctggccc ccggccagga cgaggtcttc
     1141 ttcactccgg agcaatcggg gacgacggga acgatcttcg atcactgccg tcgtgccctc
     1201 gatcgggggc tctctctcat tggcgaacgt ggtctgccgc tgatcgggac cggcgactgg
     1261 aacgacgggt tcaaccgggt ggggcgagag gggcgagggg agagcgtgtg gctcgggtgg
     1321 ttcctcgacg ccaatttgag gcgtttcgcc gagatcgccg agggactggg ggaggctgag
     1381 ctcgcctcgc gctggcggca aggggcacaa cgtctccgct caagcatcga agcggaggct
     1441 tgggacggcg actggtaccg gcgcgccttt ttcgacgacg ggacgccgat cggctcgtcg
     1501 atgagtcctg aggcccgtat cgactcgatc gctcagtctt ggtcggtgat ccacggtggc
     1561 ggcatagagc gacgcgcagg gcgggccatg gagtcggtgg aggagtacct ggtccatcgg
     1621 ggcgacggtc tggtcctcct gttcgatccg cctttcgacg agtgggaggt cgatccgggt
     1681 tacatcaagg ggtacctccc cggagttcgc gagaacggag ggcaatacac ccacgccgcc
     1741 gtctggtgtg cgatcgcctt cgccaccttg ggcgacggtg accgagccgg tgagttgttc
     1801 agtctcctca atccaatcaa ccatgccagc accagggccg ggattcaccg ataccgggtt
     1861 gagccctatg tggcggctgc cgacgtgtac tccaaggctc cccatgtcgg caggggcggc
     1921 tggacctggt atacgggctc ggcagggtgg atgtaccgag ccggtgtgga gtggatcctc
     1981 gggtttcgtc ttcgggggtc tcgtctggtt atcgacccct gcataccccg cgcctgggga
     2041 gagttcgaga ttgccttccg gtaccactcc tcccgctatg agataagagt cagcaaccca
     2101 aatgcagtca gccggggggt ggtgagcctg gagatggatg gcgcggtgct tccctatggc
     2161 gacggactcg atctcctcga cgacggctca acgcatcagg ttcgggtgac tctgggctga
     2221 gtggagccga gcccgaccct cgcgctccgg gggcagagag tccgcgtctg tgcttcaccc
     2281 ggatcgccag gattcgacta gcggctcgct ggccgccctg tcggtggtgc ggttggcgtg
     2341 ctcgggctac tcgggggcgc gaggtcacgt gaggacggtt tcttcaccta gctcgtcggt
     2401 gatggttgct tcgacgtcgt agtcgatctc gaggacgatg agggagggga ttggcagggt
     2461 gattgcttca cggagcagcc cgccgaactc gttggcggtc gtcactctcc aagcggcaag
     2521 cccgaatgat tcggccaggg caacgaagtc gggattggtg aagtcgacgc cgaagtggct
     2581 tccgaagcgc cttcgttgct tcaaggcgat ggatccgaac tgtctgtcct cccagatcac
     2641 agtcacgaag ggggtgctca gccggaccgc ggtctcgagc tcctgtacgt tcatcaaaaa
     2701 cccgccgtct ccgcacactg tgacgacctt gcgctcgggt tggaccaact tggcggcgat
     2761 ggcggccggc agggcaaagc ccatgccggc gagcccgttg gcgatcagga ccgtgttcgg
     2821 ttcgtgtgct gggaacatgc gcccgaccca gagcttgtgc aacccgacgt cggagatcag
     2881 caagtcggag cgacccagag catcgcgtgt gtcgtagagg acccgaggtg gtttcatcgg
     2941 gtatccctcg tctttctggg cgctgtcgaa ccggtccgcc actgcctggc gcagcttggt
     3001 ggagcctccc ggatgcggga tcagtgtgca tgctgcggcc agttggtcga gtacggcgtt
     3061 gagatccccc accagctcga cttcgggcaa gaagttctga tcgatctcgg cgggaagcgt
     3121 gtcgaggcag atgatcgtct tgtccccgga cgggttccac aggctggggg agagctcgac
     3181 gaggtcgaat ccgatcgcca cgaccacatc cgcctgatcg aacccggcca tgtcgaagtc
     3241 ccccgcctgt agacccaccg caccgaggaa caagggatca tccgggtcta tcaggccctt
     3301 gcccatgaag gtgactgccg tgggaagccc gaccgatctc acgaacgagc gcaaggcggt
     3361 gacagcccga ttccggacca ccccgttgcc tgcaagagcc acgggagagc gggcagcgtc
     3421 gaggacccgg gcagccctcg aaacgtccgc atcggagggc tcggtgcgca ccggtggttg
     3481 ccttcgcaac ggtgaaccgg agtatggctc acccatcacg tcttcgggga gctcgatgtg
     3541 tgtcggaccc ggtttctccg cctcggcgag cttgaaagcc ttacgcacga cttcggggat
     3601 gatcgaagga tccccgactc gcgcgttcca tttcgtgatc gggtccatga ggttgaccac
     3661 gtcgatgtgc tggtgagact ccttgtgcat ccgctcttgg tcggtctgcc cggtcagcgc
     3721 caccatgggg gcgcggtcta ggaaggcgtc ggcgaccccg gtcaccaggt tggtggcgcc
     3781 gggtcccagg gtccccagac agacgccggc ccgaccggtg agcctcccga ccacgttcgc
     3841 catgtacgcc cctgcctgct catgacgcac cggcacgaat tcgatcgaag acgcatgcaa
     3901 cgcacggttg agctcgagtg tttcctcgcc gggaatcccg aagacgtacc cgacgccttc
     3961 cgcctcgaga cattcaacca acaagtcggc agcggtcggg ccggtcacgg cgtggccgac
     4021 agctgtcgat ccggcgccac tatggcgatc tcagagagcg gcatttcgat gtccccagca
     4081 ttgctgctga ccctcgattg aggctgatcc atggggtctc cttcgactgc gcttccagcc
     4141 gacaaccctg atcctggcac gcttccgatg ccgaagagtc ggatggtctc tggagttgtg
     4201 gcggcgtcgg gagaaggacc cgcttccaag gctgctgggc gatcaaagcg atcggcgccg
     4261 aatggacacc tcagattggc tcgctctccc aaccttgggg cactccaata tctcccaggc
     4321 ccgagctatc ggcgccgcac agccagcggg agtcctcgct accgtctgtg aggaggtatc
     4381 cgtcctagtc gacaatcacc tcgctcttgt gcgcggcaaa ggcagccaga acctcttctt
     4441 tctctttctc gggaacaccg acgaagtcaa gggtgcgccc gagctcagcc gccacttcat
     4501 cgaattcctc cgaagagatc cgaaacttgc gatgggcctc ttccaggccc agggctgtca
     4561 accctggctc tgtggctacg tattcgaaag gcccacccgc aacgttgcag acccagagcg
     4621 tcctcatgaa cttgaggccc ggaagtctgc tcagctggtt ggtgtgccac tcccgcagag
     4681 cggggttctg cgactcttgg ccaacaatgg gattcttgac gacctcatcg ctgaagtggt
     4741 cgatcaccgc ggcgatggca aaaacgccgc cgagcctgtc gtagagggtc ttctctgtca
     4801 ttctgatcct tcttcgtcgc ggacagatcc cgtcgagtcc attacgaccg cagttggctc
     4861 gacggttcag aactcttcgc gcgcaccacc
//
LOCUS       DASUTA010000042         3677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_662959, whole genome shotgun sequence.
ACCESSION   DASUTA010000042 DASUTA010000000
VERSION     DASUTA010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662959"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VFZ80_00840"
     CDS             complement(<1..411)
                     /locus_tag="VFZ80_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HEX6286005.1"
                     /translation="MTVETTPMRPGYRQPPHSREAEESVIGAMLLSEDAVNEVMDQVH
                     PEDFYVPAHQAIFESMRELFDSSQAIDAVTVSESLRRKGELDKIGGVQYLTRLVDIVP
                     STSNVSYYAGIVEEHAKRRELIRAGASVTELAFEI"
     gene            complement(655..1032)
                     /locus_tag="VFZ80_00845"
     CDS             complement(655..1032)
                     /locus_tag="VFZ80_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286006.1"
                     /translation="MEIAAWIAAVLIAVLTVFHLALALGAPAGAAAWGGSHPGVLPTR
                     LRIASGLVAIFFYPVVAIELLHAADVIDLSGDVGSVWIWVLAGLFTLGALANFASRSR
                     IERIWGPISLVLAVCCVIIAVGM"
     gene            complement(1079..1525)
                     /gene="rplI"
                     /locus_tag="VFZ80_00850"
     CDS             complement(1079..1525)
                     /gene="rplI"
                     /locus_tag="VFZ80_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX6286007.1"
                     /translation="MKLILTADVDPLGKRGDVVDVADGYARNFLLPRQKAIKANEGAL
                     AQAEAIREARIEAERRAKEEAENIATQLVGSRVVLAAQAGDEGQLYGSVGVSDVVEGI
                     KRFTGIELDRAQVHIAEPIKAIGLHEVQVRVHADVEFPLTLDVIPA"
     gene            complement(1522..1794)
                     /gene="rpsR"
                     /locus_tag="VFZ80_00855"
     CDS             complement(1522..1794)
                     /gene="rpsR"
                     /locus_tag="VFZ80_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX6286008.1"
                     /translation="MARKNRRNPKQSPRGRRQDFRRGKPKPCPLDTEDARVDWKDTSL
                     LRKYVSDRGKIRGRRVTGLCPQHQREVAVAVKNAREMALLPYVGGR"
     gene            complement(1805..2257)
                     /gene="ssb"
                     /locus_tag="VFZ80_00860"
     CDS             complement(1805..2257)
                     /gene="ssb"
                     /locus_tag="VFZ80_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX6286009.1"
                     /translation="MSSNVTLIGNLVDDPELRFTPSGVAMAKIRMAVNRRWRGQDGEW
                     QEQTSFFSGTIWREQAEQAAESLQKGARVIVTGRLEQRSWETEAGEKRSVVEIAVDEI
                     GPSLRWATATVNRTQREDNWGGSSGGSQSAGASAPSARDDYGPDEAPF"
     gene            complement(2331..2636)
                     /gene="rpsF"
                     /locus_tag="VFZ80_00865"
     CDS             complement(2331..2636)
                     /gene="rpsF"
                     /locus_tag="VFZ80_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX6286010.1"
                     /translation="MSTGGAMRDYELMTIHRPDLAEDDVESKISELETYLSNSDAEIT
                     KRDMWGKRRFAYEIDHLSEGYYSVVSFRAGAEAVAGVDRLLTLTDEVVRHKIVRLGE"
     gene            complement(2741..3145)
                     /locus_tag="VFZ80_00870"
     CDS             complement(2741..3145)
                     /locus_tag="VFZ80_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX6286011.1"
                     /translation="MLLIAACSAQPGIDGFDSGEVAVGEAVLSVAVASTSSQRGQGLS
                     GLDELPRGLDGMLFIWDAPSMTTFHMSGVGFPLDVWFFDGDGMLIGSTSMPTCPDGAC
                     TSYSTPGPVKWALETPAGDFSFAPASRLTIEG"
     gene            complement(3163..>3677)
                     /locus_tag="VFZ80_00875"
     CDS             complement(3163..>3677)
                     /locus_tag="VFZ80_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX6286012.1"
                     /translation="AEAVKLEGGRKRLPVIEAILDAEIPVMGHVGLTPQSVHAMGGFK
                     VQGKVVDAAREMIEDAKALSDAGCFSIVIEGVPDILGELITKEVDAPTIGIGAGPSTD
                     GQVLVFHDVLGLGDGNYPKFVRPYASLADDAVAALQRFKADIQKGSFPGDDESYHVTD
                     EIAAELLKDS"
BASE COUNT          685 a         1198 c         1108 g          686 t
ORIGIN      
        1 gatctcgaaa gccaattcgg tgactgacgc tcccgccctg atcagctcgc gccgcttggc
       61 atgttcctcg acgatccccg cgtagtagga gacgttcgag gtcgacggga ctatgtcgac
      121 cagtcgggtg aggtattgga cgccaccgat cttgtcgagc tcacccttgc ggcgaaggct
      181 ctctgacacg gtgactgcgt cgatggcctg gctcgagtcg aacaactccc gcatcgactc
      241 gaaaatcgcc tgatgcgccg ggacatagaa gtcctcggga tggacttggt ccatcacctc
      301 attgaccgcg tcctcggaca ggagcatggc cccgatcacc gactcctcgg cctcacggga
      361 atgagggggc tgtcgatagc ctggccgcat cggtgtggtt tcaaccgtca tctgtgtctc
      421 gccaacaatc aagcatccat caggtacaga tttcgcggtc ttgacgagca agaccccgcc
      481 gggcggtaac gccgcaccgc cgacagcggg gggaagtggg agcaacatag gcgcgaccca
      541 gacggtgcac caaggtggag cactgcttga ttttcccgaa ctgctgattg tttttcgcgc
      601 tggtcccaga ccgcgaatct ggcggtcaaa tcgcgaaatc cgtcgctaca gcattcacat
      661 tccgacggcg atgatcacgc aacacaccgc cagaacgaga gaaatcgggc cccagatcct
      721 ctcgatcctg gagcgagagg cgaaattggc cagcgcaccc agggtgaaga gaccggcaag
      781 cacccagatc caaaccgagc ccacgtcccc gctcaggtcg atcacgtcgg cagcatggag
      841 taactcgatg gccacgaccg ggtagaagaa gatcgcgacg agaccgctgg cgatacggag
      901 ccgggtcgga agcacacccg gatgcgaccc gccccaggcg gcggctcctg ccggcgcacc
      961 tagagcgagc gccagatgga agacggtcaa caccgcgatg agcacggctg cgatccaggc
     1021 ggctatttcc acggtcgcag accctaacgc agccgtcggc gggcgccgat gtttcgagct
     1081 aggcgggaat gacgtcgagg gtcagaggga actcgacgtc ggcgtgcacc cgtacctgaa
     1141 cctcatggag cccaatggct ttgatcggct cggcgatgtg cacctgagcg cggtcgagct
     1201 cgattccggt gaaacgcttg atgccctcga ccacgtcgga caccccgacc gagccgtaga
     1261 gctgaccttc gtccccggcc tgggcggcaa gcaccactcg actgcccacc agttgggtgg
     1321 cgatgttctc ggcctcctct ttcgcccggc gctcggcctc gatgcgagcc tcccggatcg
     1381 cctcggcctg cgccagggcg ccctcgttgg cctttatcgc cttttgccgg ggcaggagga
     1441 agttacgggc gtaaccgtcc gcgacatcga cgacgtcgcc cctcttaccc aaggggtcga
     1501 catcggcggt gaggatgagc ttcatcgtcc acccacatat ggaagtagcg ccatctcgcg
     1561 ggcgttcttc accgcgacag cgacctcgcg ctggtgctgg gggcagagcc cggtgacacg
     1621 ccggccccgg atcttgcccc gatccgacac gtacttgcgc agcaaggacg tgtctttcca
     1681 atcgacgcgt gcgtcctcgg tgtccaaagg acatggcttg ggcttgccgc gtcggaagtc
     1741 ttgacgtcgc ccccgcggcg actgtttcgg gtttcttctg ttcttacggg ccatccaaat
     1801 cctcctagaa cggtgcctcg tccgggccgt agtcgtcacg tgccgatggc gccgatgcgc
     1861 cggcggactg gctgcccccg ctcgatccgc cccagttgtc ctcacgttgg gtgcggttga
     1921 ccgtggcggt cgcccaacga agagacgggc cgatctcgtc gacggcgatt tcgacgacgg
     1981 agcgcttctc gccggcctcg gtctcccagc tccgttgctc gagacgcccg gtgacgatga
     2041 ccctcgcacc cttctgcaga gattcggcgg cctgctcggc ctgctcgcgc cagatggtgc
     2101 cgctgaagaa gctggtctgc tcctgccact cgccgtcctg gccacgccag cggcggttga
     2161 ccgccatgcg gatcttcgcc attgccacac ctgatggcgt gaatcgaagc tccgggtcgt
     2221 cgaccaggtt tccaatgaga gtcacattgc ttgacatcga gttctcctca gttcttcccg
     2281 ctttcgacta tgccgttttc ccgcgacaga ttccagtcgc ttggatctcg tcactcgccc
     2341 aagcgcacga tcttgtgacg aacgacctca tcagtgagtg tgagcagtcg gtccacaccg
     2401 gcgacggctt cggcaccggc acggaacgag accacggagt agtagccctc gctcaaatgg
     2461 tcgatctcgt aggcaaatcg gcgcttgccc cacatgtcac gcttggtgat ctctgcgtcg
     2521 ctgttggaga gataagtctc cagctcggag atctttgact cgacgtcgtc ctcggccaga
     2581 tctggccgat gaatcgtcat cagttcgtaa tcgcgcattg cacctcctgt ggacatccat
     2641 tgcggaaggc cccaccaact cggtggagca ggggccggcg gcgcgtgtcg ccggatcagg
     2701 atcagaggat aaccgggatc agaccctttc cacagtccgc tcagccttcg atagtgagcc
     2761 tggaggcagg cgcgaacgag aaatcgcccg caggtgtttc caatgcccac ttgacgggtc
     2821 cgggtgtgga gtagctcgta caagccccgt cgggacaggt cggcatgctc gtcgagccga
     2881 tcagcatgcc atcgccgtcg aagaaccaga cgtcgagagg gaatccgaca ccgctcatgt
     2941 gaaaagtcgt catggaaggg gcatcccaga tgaagagcat cccgtcgagc ccacgaggca
     3001 gctcgtcgag cccactcagc ccctgacccc tctgcgatga ggttgaggca actgccacag
     3061 acaagacagc ttcgccaacc gctacctcac cggagtcgaa accatcgatc ccgggctggg
     3121 ccgagcaagc cgctatgaga agcaacagga ggagagggcg tctcagctgt ctttgagaag
     3181 ctcggcggcg atctcgtcgg tgacgtgata gctctcgtcg tcaccgggga acgacccttt
     3241 ttggatgtcc gccttgaaac gctgcaaggc agcaaccgcg tcgtcggcga gactggcgta
     3301 gggcctcacg aacttcgggt aattcccgtc tccgagaccg aggacgtcgt ggaacaccag
     3361 cacctgccca tctgtgctcg gccctgcccc gatcccaatg gtcggagcat cgacctcctt
     3421 ggtgatcagc tcacccagga tgtcgggtac tccctcgatg acgatggaga agcatcctgc
     3481 gtccgagaga gccttggcgt cctcgatcat ctctcgtgct gcgtcgacca ccttgccctg
     3541 gaccttgaaa ccacccattg cgtggaccga ttgcggggtc agcccgacgt gacccatgac
     3601 cgggatctcg gcatcgagga tcgcctcgat gacggggagt ctctttcggc cgccttcgag
     3661 tttcaccgcc tcggccc
//
LOCUS       DASUTA010000043         2225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1699360, whole genome shotgun sequence.
ACCESSION   DASUTA010000043 DASUTA010000000
VERSION     DASUTA010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699360"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VFZ80_00880"
     CDS             complement(<1..568)
                     /locus_tag="VFZ80_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009356589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX6286013.1"
                     /translation="MFRDRTDAGRKLGAALAELRSGQPIVIGLPRGGVPVAAEVAQAL
                     EAPLDVVVVRKLGLPHQPEFAMGAVGEDGVRLIDWVTVADAGVTASELRFIVEREEDE
                     VLRRAARLRGGRPPPDLDDRVVVVVDDGIATGSTATAAVRVMRDLGARMVVVAAPVAP
                     ARVVADLKRIADDVVVLETRDPFYAVGQA"
     gene            complement(581..1072)
                     /locus_tag="VFZ80_00885"
     CDS             complement(581..1072)
                     /locus_tag="VFZ80_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6286014.1"
                     /translation="MAVPTEAPFEKGSPAMNKIVLAVDGSDHSTRAAEMAGSLSQAMG
                     APVDVVHVVSDGSMLTAGPIQDYARLERIVITQRELLESIGAGVVAEAAGKVRALGGT
                     VGATKVLVGSPAHQIAEYAGEAGADCIVMGRRGLGDISGLLMGSVSHKVGHLTGLSLV
                     TTE"
     gene            complement(1160..1429)
                     /locus_tag="VFZ80_00890"
     CDS             complement(1160..1429)
                     /locus_tag="VFZ80_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286015.1"
                     /translation="MADQGRRNRILAMVAGGVVGGIVGALVAVNLMIYFGTEAGYEAS
                     LPEIFEKSAVLGVIVVAVLVAGPIVGGWAVTRMRAHRSGQVAPRR"
     gene            complement(1439..1891)
                     /locus_tag="VFZ80_00895"
     CDS             complement(1439..1891)
                     /locus_tag="VFZ80_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6286016.1"
                     /translation="MIDTSLEKGNEMSVVAGVDDSPVSKLVVERAIEQARLRGTDVHL
                     AHVAYAPMVYADVPINWAEVVEAQRNAVWSQLEPAIAASDVPVVKVDLDGYPPDSLVA
                     YANDQQASLLVVGTRGRGDFASLILGSTSHRAIHLSECDVLVVKTPEE"
     gene            complement(2000..>2225)
                     /locus_tag="VFZ80_00900"
     CDS             complement(2000..>2225)
                     /locus_tag="VFZ80_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX6286017.1"
                     /translation="IFDVLSDIAPEVDRGQVDDDVDLTEQLDLDSMDYLTWMIGISDA
                     TGIEIPQRDVSRFLTVRGAVGFLVDHAPVA"
BASE COUNT          399 a          778 c          674 g          374 t
ORIGIN      
        1 aagcctggcc gactgcgtag aacggatctc gtgtctcgag aaccacgacg tcgtcggcga
       61 ttcgcttcaa gtcggcgacg acccgggcgg gtgcgaccgg cgcggcaacc acgaccattc
      121 tcgctccgag gtctcgcatg acacgcacgg ccgcggtggc cgtggacccc gtggcgatgc
      181 cgtcgtccac caccaccacg acgcgatcgt ctaggtcggg tggcggtcgc ccccctcgca
      241 gccgggcagc gcgacgcaag acctcgtctt cttcccgctc cacgatgaag cgcaattcgg
      301 aggcagtcac gcctgcgtcg gcgacggtga cccagtcgat gagccgaacg ccgtcttctc
      361 ccaccgcacc catcgcgaac tcaggctgat gggggagacc cagcttgcgc accaccacga
      421 cgtccagcgg tgcctccagg gcctgtgcca cctccgcagc caccgggacc ccgcctcgtg
      481 gcagcccgat cacgattggc tgtcccgatc tcagttcggc cagggcagcg ccgagcttcc
      541 gaccggcatc tgttcggtca cgaaacactg gccactcctc tcactcggtg gtgaccaagc
      601 tgagacccgt caaatggcca accttgtgtg agacactgcc catcaagaga cccgatatgt
      661 ctccgagacc gcgacgtccc atgacgatgc aatcggcacc agcctctccg gcatactcgg
      721 cgatctggtg cgccggagac ccgacgagca ccttggttgc gcccaccgtc cctcccaggg
      781 ccctcacctt gccggcggct tcggcaacga ctccggcgcc aatcgactcg agaagctcac
      841 gttgggtgat cacgattcgc tcgagacgcg catagtcctg aatcgggccg gccgtcagca
      901 tgctcccgtc cgagacgacg tgcacgacat cgacgggcgc acccatcgct tgggacagac
      961 tccccgccat ctcggcggca cgtgtcgagt gatccgagcc gtcgacagcg agaacgatct
     1021 tgttcatagc cggcgacccc ttctcgaagg gcgcctccgt agggacggcc atgattcctc
     1081 ctggttcgtc tctatcgaga ctgcctcctg gggcgtccac ggcgcaagag tcgaaggacc
     1141 tgactcttct cggtgagggc tagcgccggg gagcaacctg cccggacctg tgcgcgcgca
     1201 tcctcgtgac cgcccagccc cccacgatcg gaccggccac caggacggcg acgacgatca
     1261 cgccgagaac ggcgctcttc tcgaagatct ccgggaggct ggcttcgtat ccggcctcgg
     1321 tcccgaagta gatcatcagg ttgaccgcca ccaaggcgcc cacgatgcca ccaaccactc
     1381 ctccggccac catggccagg atccggtttc gtcgtccctg atcggccatg tcgaacccct
     1441 attcctctgg ggtcttcacg acgaggacgt cacactcgga gagatgtatc gcccggtggc
     1501 tggtgctgcc gaggatgagc gaggcgaaat caccgcggcc acgggtcccc accaccagaa
     1561 gcgaagcttg ctggtcgttg gcatacgcaa ccaggctgtc cggggggtag ccgtcgagat
     1621 cgaccttgac gacgggcaca tcggatgcgg cgatggcggg ctcgagctgg gaccagacgg
     1681 cattgcgctg cgcctcgacc acctctgccc agttgatggg gacgtcggca tagaccatcg
     1741 gggcataggc gacgtgtgcc aggtgcacgt ccgtgcctct caatctcgcc tgttcgatcg
     1801 ccctttcgac aaccaacttc gaaaccgggg aatcatcgac accggcgacg acgctcatct
     1861 cgtttccttt ctcgaggctc gtatcgatca tcacacgaat cgccgctggc gaccacggga
     1921 cgaagtcaca gccagccggg tcatacgccc ctgatggact gacctgggcg ccttttggcg
     1981 ccggcggaca ccgggccggt caggcgaccg gggcgtggtc caccaagaag cccacggctc
     2041 ccctcacagt caggaaccgc gacacgtcgc gttgggggat ctcgatgccg gttgcgtcac
     2101 tgatcccgat catccaggtc agatagtcca tggagtcgag gtcgagctgt tcggtgagat
     2161 cgacgtcgtc gtcgacctga ccccgatcga cctcgggggc gatgtcggag aggacgtcga
     2221 agatc
//
LOCUS       DASUTA010000044         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_274534, whole genome shotgun sequence.
ACCESSION   DASUTA010000044 DASUTA010000000
VERSION     DASUTA010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274534"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VFZ80_00905"
     CDS             <1..577
                     /locus_tag="VFZ80_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX6286018.1"
                     /translation="TRIAAQAGFHVHAAARVGGLYDLVVPTGNFGNVYSAWIAKQMGA
                     PIDTITIANNANHALSDLVDTGRMSQSDVLPTMAPAMDIQVPSNLERFTGDPTKEFRA
                     SWSSDTQIAQTIRDVERAHGYRLDPHTATAWRAAEKTRGDLPQLVVATAHPAKFDEDV
                     PPPQWFPSLDGLEERQVTIDADTSELHHLIR"
     gene            763..>2065
                     /gene="rho"
                     /locus_tag="VFZ80_00910"
     CDS             763..>2065
                     /gene="rho"
                     /locus_tag="VFZ80_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX6286019.1"
                     /translation="MTTRAQLQSKSIDELREVASAAGIETEGLQKSKLIAALMDAGEV
                     TESKPIDAVDLPKATPRSDKSESRSDDNDDDDSRDDNRGGDDNRGGDDNRGGGDNRGG
                     GRQRNRKRRDNDDRVPEDQLEVREGILDILPEGYGFLRVTGYKSGDKDVYVSANQVRK
                     FRLRKGDIISGPIRPPRSKEKFPAVVRISSVNGMDPDEAMNRPKFESLTPLFPDERLR
                     LEVEGKSNNILTRIVDLIAPIGKGQRGLIVSPPKAGKTTVLQEIAQSITANNPECYLM
                     VVLVDERPEEVTDMQRTVKGEVIYSTFDRPAEEHTQVSELAIERAKRLVEMGTDVVIV
                     LDSITRLARAHNLATPASGRILSGGVDSQALYPPKRFYGAARNIEEGGSLTILGTALV
                     ETGSKMDEVIYEEFKGTGNSEVYLVRQLADRRMFPAYEINRS"
BASE COUNT          451 a          684 c          605 g          325 t
ORIGIN      
        1 gacgaggatc gctgctcagg ccggtttcca cgttcatgca gctgcccgtg tcgggggcct
       61 ctacgacctg gtggtgccca cggggaactt cggaaacgtc tattcggcgt ggatcgccaa
      121 gcagatgggt gcgcccatcg acacgatcac gattgccaac aacgccaacc acgccctcag
      181 cgatctagtc gacaccggcc ggatgagcca gtccgatgtc ctcccgacaa tggcgcctgc
      241 catggatatc caggtgcctt ccaacctcga gcgcttcacc ggtgacccaa ccaaggagtt
      301 cagagcgagc tggtcgagcg acacgcagat cgcccagacc atcagggacg tcgaaagggc
      361 ccacgggtac cgactcgacc cgcacacggc aacggcctgg cgagccgccg aaaagactcg
      421 cggcgacttg ccccaactcg tggtggctac cgcgcacccg gcgaagttcg acgaagacgt
      481 gccgccaccg cagtggtttc cctccttgga cgggctcgaa gagcgtcagg tgacgatcga
      541 cgccgatact tcagagctac accatctcat ccgataactc agttcgccgg gctgctttgc
      601 gcaaccgggt gaagcgacta ccatgacagc cgcagccaca ggacgtgtct gcatccccgc
      661 ccaactcacc cagagatccc tgtgtgaacg atccctgcgt gacgtgccgg gagcaacctc
      721 cgatccgacg atccctgtgg ggacagatca cataaggatc caatgacgac acgagctcaa
      781 ctccagagca aatcaatcga cgaactccgt gaggtcgcgt ccgcggccgg aatcgagact
      841 gagggccttc agaaatccaa gctcatcgcc gccctgatgg acgctggtga ggtcacggaa
      901 tcgaagccca tcgacgccgt cgatctacca aaggcaacgc cacgctcgga caagtccgag
      961 tcgaggtccg acgacaacga cgacgacgac agtcgcgacg acaaccgagg cggcgacgac
     1021 aaccgaggcg gtgacgacaa tcgcggcggc ggcgacaacc ggggtggtgg tcgtcaacgc
     1081 aatcgcaagc gtcgtgacaa cgacgatcgc gttccagagg accaactcga ggtccgagag
     1141 ggaattctcg acatccttcc agagggttac gggttcctcc gtgtcaccgg ttacaagtcg
     1201 ggagacaagg acgtctacgt ctctgccaac caggtccgca agttccggct tcgcaagggc
     1261 gacataatct ccggcccgat ccgcccgccg cggtcgaaag agaagttccc cgctgtcgtc
     1321 cggatcagct cggtcaacgg gatggacccc gacgaggcga tgaaccgccc caagttcgag
     1381 agtctcacgc cgctgttccc cgacgagcga ctccgtctcg aggtggaagg gaagtcgaac
     1441 aacatcctca ccaggatcgt cgacctcatc gcccctatcg gcaaaggtca gcgcggtctg
     1501 atcgtgtcac cccccaaggc cgggaagacc acggttctcc aggagatcgc tcagtcgatc
     1561 accgccaaca accccgagtg ctacctcatg gtggtgctgg tcgacgagag acccgaagaa
     1621 gtcaccgaca tgcagcggac ggtcaagggc gaggtcatct actcgacctt cgaccgacca
     1681 gcggaggagc acactcaggt ctccgagttg gcgatcgaac gggccaagag actcgttgag
     1741 atgggcaccg acgtggtgat agtcctcgac tccatcaccc gtctcgcccg ggcccacaac
     1801 ctggcgacgc cggcctccgg gcggattctc tccggtggtg tcgattccca agctctctat
     1861 ccgcccaagc gcttctacgg tgcggcccgc aacatcgaag aaggcggctc gttgacgatc
     1921 ctcggcaccg ccctcgtgga aaccggctcc aagatggacg aggtcatcta cgaggagttc
     1981 aagggcaccg gaaatagtga ggtatacttg gtccgccagc tcgccgaccg ccgcatgttc
     2041 ccggcctacg agatcaaccg ctcgg
//
LOCUS       DASUTA010000045         2338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_663969, whole genome shotgun sequence.
ACCESSION   DASUTA010000045 DASUTA010000000
VERSION     DASUTA010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663969"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..>2338)
                     /locus_tag="VFZ80_00915"
     CDS             complement(62..>2338)
                     /locus_tag="VFZ80_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX6286020.1"
                     /translation="IKRRLFGRPFATYEEESQLLPKRLALPVFASDPLSSVAYATEEA
                     MLVLALVGTAAFSLLTPISLAIATLLLIVIVSYRQTIKAYPDGGGAFIVANDNLGIRT
                     GTVAASALLTDYVLTVSVSVAAGVAAITSAAPDLIPWRVEIALGFVALLTIANLRGVK
                     EASTLFAAPTYLFVITVGIMLITGFVQCFDGECPKAISSGLDIEAEVGAIGLFLVLRA
                     FASGSTALTGVEAIANGVQAFREPKAHNAATTLGIMGAISISMFLGISTLARWFDVRI
                     SEETIDEFGTVISQIGRAVFDGGFGFYALQVFTAAILILAANTAYQDFPRLSAILARH
                     KLTPRQYLNRGDRLVFSNGIVTLAVVAAALLVIFDAEVSRLIQLYVVGVFTAFTLSQA
                     GMVRHWLRTKEPQWQRSVAINSIGAATTGIVLVVIATVKFAHGAWLVIVAVPLLVALM
                     LSIRRHYLSVASQLKRVPVEAVPQSTRILVLVAHHDEGTDKALRYAELLNAEAITCVH
                     AEEPRSDDLIYTWDAGHPRHALEVLPGEKESISQRVANRVRRERGTYPDSRITVIMAD
                     RVRIRSILAPFAHRHSLAIKSRLLFEPGVVVTDLNVLRRSRRGRLQAVPVGRIEQVVL
                     ISDMTRPIREALTYAQSLGPPVTAVHIDVDTAQRERLEQQWQQAGYEFELEVIPSPYR
                     SIVDPLVTYLRERRRAATPGTLISAVIPEFVVPGRVTQLLHNQTGLAIKGVLAAEPGI
                     AVTSVPFHLLPKASSDIQ"
BASE COUNT          463 a          691 c          772 g          412 t
ORIGIN      
        1 gggatggggt atcgcttcga accggcatcg ggttcgaacc gtcggggctg acccggatac
       61 ctcattggat atcgctgctc gccttgggga gcagatggaa tggcacgctg gtcaccgcga
      121 taccgggttc cgccgccagc acgcctttga tcgccagccc ggtctggttg tggagaagct
      181 gggtgaccct cccgggcaca acgaactcgg ggatcacagc cgagatcagg gtgcccggtg
      241 tcgcggcacg gcgtctctcc ctcaagtagg tgactagcgg gtcgacgatg ctccggtaag
      301 gactggggat cacctcgagc tcgaactcat accctgcctg ctgccactgc tgctccaatc
      361 tctccctttg ggcggtgtcc acgtcgatgt ggactgccgt gaccggcgga ccgagggatt
      421 gcgcgtaggt cagggcctcg cggatcggcc tagtcatgtc ggagatgaga acaacctgct
      481 cgatccgacc cactggcact gcttgaagcc gccctcgtct cgagcgacgg agcacgttca
      541 gatcggtcac cacgacgcct ggctcgaaca gcagtcgtga cttgatggcg aggctgtgtc
      601 ggtgggcgaa tggagccagt atcgaccgga tccgcacccg gtcggccatt atgacggtga
      661 tcctcgaatc ggggtaggtt ccacgttccc ggcgtacccg gttcgcgacc cgttgcgaga
      721 tcgactcctt ctcaccgggc aggacttcca gagcatgtct tgggtggccg gcgtcccagg
      781 tgtagatcag gtcgtccgat cgaggctctt cggcgtgaac gcaggtgatt gcctcggcat
      841 tgagcaactc ggcatagcga agtgccttat cggttccttc gtcgtgatgg gccaccaaca
      901 cgaggatccg cgtcgactga ggcaccgcct cgacagggac gcgtttcagt tgcgaagcga
      961 cactcaagta gtgacgtcga atgctcaaca tcagggcgac gaggagagga accgccacga
     1021 tcaccagcca cgccccatgt gcgaacttca cggtggcgat gacgaccagc acgatcccgg
     1081 tcgtcgccgc tccgatggag ttgatcgcga ccgagcgctg ccattgcggt tccttcgttc
     1141 tgagccagtg gcgaaccatg cccgcctggc tcagggtgaa ggccgtgaag actcccacca
     1201 cgtagagctg gatcagtctg ctcacctcgg cgtcgaagat caccaggagg gcggcagcga
     1261 ctactgcaag agtgacgatc ccgttggaga agacgaggcg gtcgccgcgg ttgagatact
     1321 ggcggggagt cagcttgtgt ctggcgagga tcgcggacaa tctcgggaaa tcctggtacg
     1381 cggtgttcgc ggccaggatc agaattgcgg cggtgaacac ttgtagcgca tagaagccga
     1441 agccaccatc gaacaccgcc cgtccgatct gagagatcac cgtcccgaac tcgtcgatcg
     1501 tctcctcgga gatgcgcacg tcgaaccatc gagcgagagt ggagatcccg aggaacatcg
     1561 agatcgagat cgctcccatg atgcccaggg tggtggcggc gttgtgtgct ttcggctccc
     1621 ggaaagcctg gacgccgttg gcgatggcct cgacaccggt cagggcagtg gacccggagg
     1681 cgaacgcccg aagcacaagg aacaaaccga tcgccccaac ctcagcctcg atgtcgagac
     1741 ccgaagagat cgccttcggg cactcaccgt cgaagcactg gacgaaaccg gtgatcagca
     1801 tgatccccac ggtgatgacg aagaggtagg tgggcgcggc gaagagggtg gatgcctcct
     1861 tcacacctcg gagattggcg atggtgagga gggcgacgaa gccgagggcg atctccaccc
     1921 tccaagggat gagatcggga gcggctgaag tgatggcggc gacacctgct gccaccgaca
     1981 cagagacggt cagcacatag tcggtcaaca gtgccgaggc ggccacggtc ccggttcgga
     2041 tcccgaggtt gtcgttggcg acgatgaacg ctccgccacc gtccgggtag gccttgatgg
     2101 tctggcgata cgacacgatg acgatcaaca gcaacgtggc tatggccagg gagatcggtg
     2161 tcagcaggct gaacgcagct gtcccgacca gggccaggac cagcatcgcc tcttcggtcg
     2221 cgtaggcaac cgaagacaac gggtcggaag cgaacaccgg gagggcgagg cgcttgggta
     2281 ggagttggct ttcttcctcg taggttgcga agggccgacc gaagagcctt cgcttgat
//
LOCUS       DASUTA010000046         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_793739, whole genome shotgun sequence.
ACCESSION   DASUTA010000046 DASUTA010000000
VERSION     DASUTA010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793739"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="VFZ80_00920"
     CDS             complement(<1..943)
                     /locus_tag="VFZ80_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6286021.1"
                     /translation="MKRTRLLALLAVLAMLVAACGGGDGETTTTADGGDDGVTTTTSD
                     STEPTEPPAGGEAGSGGNLLLLQWQAVTHLNPYTGTGTKDIQGASLVIEPLVGYNSDG
                     QIVPRLVTEVPSVENGGISEDLTSITYNLLPDLMWSDGTPFTADDVVFTWEYCTGSEG
                     CGSSAFGAVESVEAVDESTVTINFTGPTPWPFVPFTGGTEPVLQRAQFEACVGEAFVA
                     CEANLNPVGTGPYMVTDMRVDDTVLYEMNPNYRKIAEGKPFFGTVEIKGGGTADDTAR
                     SVLSTGEADYAWNLQVAPDILSQMEAEGNGVVDVGFNT"
     gene            complement(1071..2084)
                     /locus_tag="VFZ80_00925"
     CDS             complement(1071..2084)
                     /locus_tag="VFZ80_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286022.1"
                     /translation="MTPPNGEVLVSVRDLKKHFPIMKGVFRRQVGSVRAVDGITFDVY
                     KGETLGLVGESGSGKSTTGRVILQLEDATAGHVEFEGVDLTEMTGDKLRSLRPRMQMI
                     FQDPHASLNPRMTVARIVGEPLTEHNRSKGEDRRKRIEELLSLVGLQAKHANRYPHEF
                     SGGQRQRIGIARAIALNPDFIVADEPIAALDVSIQAQVVNLLEDLQDVLGLTYLFISH
                     DLSMVRHIADRVAVMYLGKIMELADVDTLYNNPQHPYTQALLSAVPVPDPVIEAKRER
                     VILEGDIPSPANPPPGCPFNTRCPAAEERCVIEVPAWRELEPGHWVACHFAENVHLVS
                     LPT"
     gene            complement(2098..>2688)
                     /locus_tag="VFZ80_00930"
     CDS             complement(2098..>2688)
                     /locus_tag="VFZ80_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286023.1"
                     /translation="ELLHLVGIPDAEARLKNYPHELSGGMRQRAMIAMALACDPEILI
                     ADEPTTALDVTIQAQIIQIVRDLREKLGTAVIWITHDLAVIAGLAERVIVMYGGQIVE
                     EAPVNDLYSKPQHPYTEGLLGALPRLDQKGSELVNIKGQPPSLYAYPTSCPFSPRCPY
                     VMDKCIAENPPLLDIGNGRKTACWWDMENDRPRYGN"
BASE COUNT          466 a          821 c          865 g          536 t
ORIGIN      
        1 aggtgttgaa gcccacgtcg acgacgccgt tgccctctgc ctccatctgg ctgaggatgt
       61 ccggtgccac ttgcaggttc cacgcgtagt cggcctcacc ggtcgacagc acggaacgtg
      121 ccgtgtcgtc ggcggtgccg ccacccttga tctcgacggt gccaaagaag ggcttgcctt
      181 cggcgatctt gcggtagttc gggttcatct cgtagaggac ggtgtcatcc accctcatgt
      241 cggtgaccat gtacggaccg gtcccgaccg ggttcaggtt cgcctcacat gcgacgaagg
      301 cttctccgac acatgcttcg aactgcgccc tctgcaggac tggctcggtg ccacccgtga
      361 acgggacgaa cggccaaggg gtcgggccag tgaagttgat ggtcaccgtc gactcatcga
      421 cggcttcgac actctccacg gcgccaaacg ccgaggaacc acagccctca ctaccggtgc
      481 agtactccca ggtgaacaca acgtcgtcgg ccgtgaacgg tgtcccgtcc gaccacatga
      541 gatcgggcag caagttgtag gtgatggagg tcaggtcctc cgagatgccg ccgttctcca
      601 ccgacggaac ttccgtgacc aatctgggca cgatctgtcc gtccgagttg tatccgacca
      661 gaggctcgat caccagggaa gctccctgga tgtccttggt accggttccc gtgtaggggt
      721 tgagatgcgt cactgcctgc cactggagca gcaggagatt tccgcctgag cccgcctcac
      781 cgccggctgg cggctcggtc ggctctgtcg agtcagatgt ggtggtcgta accccgtcat
      841 cgcctccgtc ggcggtggtg gtcgtctcac cgtcgccgcc gccacacgcc gcgaccaaca
      901 tggccaatac ggcgaggagc gccagcaatc tggttctctt cattatttat ttcctccata
      961 tgtcggaacg atcgtccgac attgcacacg acgctaacac cgtttgcctg ctttttgggc
     1021 gggaaaggag gcttttcgag ggtgccctca tcaagcccct gtccgtacgg tcaggtgggc
     1081 agagagacca ggtgaacgtt ctcggcgaaa tggcacgcaa cccagtgccc tggctccagc
     1141 tcacgccacg caggcacttc gatcacgcac ctctcctcgg ccgcgggaca tcgggtgttg
     1201 aacgggcaac ccggaggcgg gttggccggc gaggggatgt ctccttcgag gatgacccgc
     1261 tcccgtttcg cctcgatgac cggatcgggg acggggacgg cggagaggag agcctgggtg
     1321 taggggtgct gagggttgtt gtagagggtg tcgacgtcgg cgagctccat gatcttgccc
     1381 agatacatga ctgccacacg gtcggcgatg tgacgcacca tcgacaggtc gtgactgatg
     1441 aacaagtagg tgagcccgag cacgtcctgg aggtcctcga gcaagttgac gacctgagcc
     1501 tggatggaca cgtccagagc ggcgatcggc tcgtcggcga ctatgaagtc gggattgagc
     1561 gcaatggcgc gggcgatccc gatgcgctgc ctctgaccac ccgagaactc gtgggggtag
     1621 cggttggcgt gcttggcctg cagtccgacc agggagagca gctcctcgat cctcttccgc
     1681 cggtcctcgc ctttcgatcg gttgtgctcg gtcaggggct cacccacgat gcgcgccacc
     1741 gtcatccgcg ggttcaagga ggcgtgtgga tcctgaaaga tcatttgcat tcggggacgc
     1801 agcgatcgca gcttgtcccc ggtcatctcg gtgaggtcga cgccttcgaa ttcgacatgg
     1861 cccgcggtgg catcttcgag ctggagtatc acccgaccgg tcgtcgactt gcccgacccc
     1921 gactcgccca ccaggcccag cgtctctcct ttgtagacat cgaaggtgat gccatccacg
     1981 gctcgaactg accccacttg acgtctgaac acccctttca tgatcgggaa gtgcttcttc
     2041 aggtcccgaa cgctgaccag gacctcgccg ttcggaggcg tcatcgtggt tggagtctca
     2101 gttgccatag cgcgggcgat cgttctccat gtcccaccag cacgccgtct tccggccatt
     2161 gccgatgtcg agcagtggag ggttctcggc gatgcatttg tccatcacgt agggacaacg
     2221 cggcgagaag gggcagctcg tgggataggc gtagagactg gggggctgac ccttgatgtt
     2281 gaccagctcg gagccttttt ggtcgaggcg aggaagggcg ccgagcaatc cctccgtata
     2341 gggatgctgg ggtttggaat agagatcgtt gaccggggcc tcctccacga tctggccgcc
     2401 gtacatgacg atgaccctct cggccagtcc ggcgatgacg gcgaggtcgt gcgtgatcca
     2461 gatgaccgcc gttcccagtt tctcgcgcag gtcccggacg atctggatga tctgggcctg
     2521 aatggtgaca tcgagggccg tggtcggctc atcggcgatg aggatctcgg ggtcgcatgc
     2581 cagagccatg gcgatcatgg cgcgctggcg catcccgccg gagagctcgt gggggtagtt
     2641 cttgagccga gcctcggcat cggggatgcc cacgagatgg agcagctc
//
LOCUS       DASUTA010000047         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_666007, whole genome shotgun sequence.
ACCESSION   DASUTA010000047 DASUTA010000000
VERSION     DASUTA010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666007"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /gene="rpsO"
                     /locus_tag="VFZ80_00935"
     CDS             <1..237
                     /gene="rpsO"
                     /locus_tag="VFZ80_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006548743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX6286024.1"
                     /translation="VEEFGTHGSDTGSPEVQVALLTERINHLTEHLRDHKHDHHSRRG
                     LLLMVGKRRRLLDYLRREDVERYRSLIAKLGLRR"
     gene            445..>2200
                     /locus_tag="VFZ80_00940"
     CDS             445..>2200
                     /locus_tag="VFZ80_00940"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX6286025.1"
                     /translation="MPETSVRGNIGGKELVLSTGKLAGQANGAVVAKIGGTEMLVTAT
                     ANAKPREGVDFFPLTVDFEERMYSVGRIPGSFFRREGRPSEQAILTCRLIDRPLRPSF
                     AEGYRNETHIVVTTLSVDQENPFDVVALNGASAALSVSGPPFEGPLGGVRLGLKKGEW
                     VPFPTEEELDESVFELVVVGRRNDDGQIDIVMVEAGATENGARLVADGDSPSDEDAVA
                     DGLEQAKQYIAQLIDLQNEMVGQVEVPGFEWTPVVDYTDDIFARVEPLASSRIPAIIE
                     IAGKHERQDAEVEALDAVIAEIGVEEGDVETLKQAKAAFRSVFKKLARKRVVEQGVRM
                     DGRGASDIRPLTIEVGTIERTHGSALFQRGETQVLNITTLGMLKMEQMLDTLDIAEGK
                     RYMHHYNFPPFSTGEAGFMRGPKRREIGHGALAEKALLPVIPTSDEFPYALRLVSEVL
                     ASNGSSSMASVCGSSLSLMDAGVPISAPVAGIAMGLINEGDQFVTLTDILGAEDALGD
                     MDFKVAGTSEMITALQLDTKISGLPSEVLKSALDQAREARLFILDAMSQVISAPREEM
                     NQWAPRIESIEIPKDKIGE"
BASE COUNT          425 a          710 c          688 g          377 t
ORIGIN      
        1 gtcgaagagt tcggcaccca cgggagcgat acaggctcac ccgaggtcca ggttgctctg
       61 ctcaccgagc ggatcaatca cctgaccgag catcttcgcg accacaagca cgaccaccac
      121 agccgtcgtg gtctgttgct catggtcgga aagcgccgga gactcctgga ctacctgcgt
      181 cgagaagacg tagagcggta caggagcctc atcgccaaac tcggcttgcg gcgctagaga
      241 aaactctcga ggagcgggtc accgctcctc ttgtgtatgc gggatggcaa gccatcccgc
      301 gcaggggggc cgagattcgg cccgaagaag agagccacgc cggttgtcag tggcggcgtc
      361 tcggcccgaa tcccacggga ttcacgttcg agacgcgacc actgccaacg ggcgaccggc
      421 tccgagacaa acaaggagca caaagtgccc gaaacctctg tacgaggaaa catcggcggc
      481 aaagagctcg ttctgagcac cggcaagctt gccggccagg cgaacggtgc cgtcgtcgcc
      541 aagattggcg gaaccgagat gctggttacc gccaccgcaa acgcaaagcc cagggaaggg
      601 gtcgatttct tcccgctcac ggtcgacttc gaggagcgca tgtactccgt cggccgcatc
      661 cccgggtcgt tcttccgtcg tgaaggccgt ccctccgagc aggccatcct cacctgccgg
      721 ctcatcgaca ggcctctgcg cccgtctttc gccgagggtt atcgcaacga gactcacatc
      781 gtggtcacca ccctttcggt cgaccaggag aaccccttcg atgtcgtcgc cctcaacggc
      841 gcctcggctg cactgagtgt cagcggaccc cccttcgagg ggccactcgg tggtgtccgt
      901 ctcggtctca agaagggcga atgggttcct ttcccgaccg aggaagagct cgacgaatcg
      961 gttttcgagt tggtcgtcgt gggccgtcgc aacgacgatg gtcagatcga catcgtgatg
     1021 gtcgaggcag gcgccaccga gaacggtgcc cgtctcgtcg ccgatggtga cagcccctcc
     1081 gacgaggacg cggtcgccga cgggctcgag caggccaagc agtacatcgc ccagctgatc
     1141 gatctccaga acgagatggt gggccaggtg gaggtgcccg gcttcgagtg gactccggtc
     1201 gtcgactaca ccgacgacat cttcgccaga gtggagcctc tggcctcgtc gcggattccc
     1261 gcgatcatcg agatcgccgg taagcacgag cgccaggatg ccgaagtcga agcgctcgac
     1321 gctgtgatcg ccgagatcgg agtcgaagaa ggcgacgtcg agaccctcaa gcaggccaag
     1381 gcggccttcc gctcggtgtt caagaagctg gcccgcaagc gcgtggtcga gcagggagtc
     1441 cgcatggacg gacgtggcgc gagtgacatc cgccccctga ccatcgaggt cgggacgatc
     1501 gagagaaccc atggatctgc cctcttccag cgtggcgaga cccaggtgct gaacatcacc
     1561 acactcggca tgctcaagat ggagcagatg ctcgacaccc tcgacatcgc cgagggcaag
     1621 cggtacatgc accattacaa cttcccaccc ttctccacgg gtgaggcggg gttcatgcgt
     1681 ggtccgaagc gtcgtgagat cggtcacggt gccctcgccg agaaggcgtt gttgcccgtg
     1741 atcccgacat ccgacgagtt cccgtacgcc ctccgtctcg tctccgaggt gttggcgtcc
     1801 aatggctcgt cgtcgatggc gtcggtctgc ggctcgtcgc tctccctgat ggatgcgggt
     1861 gtgcccatca gtgccccggt cgccggtatc gccatgggtc tcatcaacga aggcgaccag
     1921 ttcgtgaccc tcaccgacat cctcggagcc gaggacgccc tcggggacat ggacttcaag
     1981 gtggccggca cctccgagat gatcacggcg ctgcagctcg acacgaagat ctcgggtctg
     2041 ccgtcggagg tcctcaaatc ggcactcgac caggctcgcg aggcccgtct gttcatcctc
     2101 gacgccatgt cccaggtgat ctccgcacca cgtgaggaga tgaaccagtg ggctccccgc
     2161 atcgagtcga tcgagatacc caaggacaag atcggtgagg
//
LOCUS       DASUTA010000048         2880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_666235, whole genome shotgun sequence.
ACCESSION   DASUTA010000048 DASUTA010000000
VERSION     DASUTA010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666235"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="VFZ80_00945"
     CDS             complement(<1..1263)
                     /locus_tag="VFZ80_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286026.1"
                     /translation="MIDGRHIADGSTLEADICIVGAGVAGISLAMEFIGRPEKVLLLE
                     GGGLSFTKSLRDLPTVVRRHSVGEQELAKGVNAGHVYYPLRFTRARAFGGSSRAWHAG
                     RGLQARPLDSLDFEARDRFPDHGWPFDRSHLDPFYRRAHERCALGPFTYETADWQAQG
                     YGAPLRLDPEVVRSEIFQFGKDSRFDRYEPDLVDAENMTLVLHANAVHLGDSRGRVDR
                     IDCATLSGNRFVARARTFILAAGALETARLLLVSRDSQPNGIGNDRDLVGRYFMEHPD
                     LAVGYLIPDPKIDREAFGLYRHQHVSESVTIEAMFRLSDATLRNEGLLNAALRLRPTY
                     RSAMAPAVSSGQTVRRSVHHGVPTPRLMGHAAKTALGARQVTRHLANKRSGHPPDVFG
                     LDVMAEQAPSWSSRMRLGRRRDRLGLPVT"
     gene            complement(1288..>2880)
                     /gene="asnB"
                     /locus_tag="VFZ80_00950"
     CDS             complement(1288..>2880)
                     /gene="asnB"
                     /locus_tag="VFZ80_00950"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX6286027.1"
                     /translation="IDRALASFNGMFAFALWDKRDRTLTLVRDRLGIKPLYFGNVNGA
                     MVFGSELHAIAAYPNADLTINRDWLDRYLREIHIRSDETIYSQIQAVRPGTIVTFDAT
                     LQAKERVYWSLIETVEAGRRDRFTGEAADAADMLDQLLGDSVRLRTVSDVPLGVLLSG
                     GIDSSSVLALLQHQSPRRVHSFSIGFPHSGLDEAPAAREIAAHIGSDHTELYMAPEDI
                     IDLIPTLARVTDQPQSDPSYVSNHAAYRLAGETVTVTLAGDGGDELFAGYHRHRWLPR
                     VWQRMGWLPVGAREQAARAIAAVPPGSWDKAFDVMQPVIPPRFRERTPGAKLHRLARW
                     MTHASPEDMYRVLSTRWDAEDRLIVAEDPDRYPMEFELAPIDGDVEILERVLAHELTA
                     TFVDTQLMKVDRASMAASVEARVPFMDHRMVEFAFRLPPEMKVRGETGKYILRQVLHR
                     YVPETLYQRPKMGFHLPLGSWLRGPLRDWAESLLDADRIRQEGLLDPEPIRVKWEEHL
                     SGRRDRVDHLWQVLMLESWLRK"
BASE COUNT          525 a          900 c          933 g          522 t
ORIGIN      
        1 cgtaaccggg agccccagac gatctctgcg tcgaccgagt cgcatccgag aagaccacga
       61 aggggcttgt tccgccatca catcgaggcc gaagacgtca ggcgggtgcc ccgagcgctt
      121 gttcgcaagg tggcgggtga cctgacgggc ccccaacgcc gttttcgcag cgtgacccat
      181 cagacgtggc gtgggcacgc cgtggtggac cgatcgccgg actgtctggc ccgagctgac
      241 ggccggcgcc atcgcagacc gataggtggg tcgcagacgc aacgcggcat tgagcaggcc
      301 ctcatttcgc agggtggcat cactgagccg aaacatcgct tcgatcgtga cgctctcgct
      361 cacgtgttga tggcgataca gaccgaaggc ctctctatcg attttggggt cgggtatcag
      421 atagccgacg gcgaggtcag gatgctccat gaagtatcgc ccgaccagat ctcggtcgtt
      481 ccctatgccg ttcggctgac tgtcgcggga aacgagcaac agccgagcgg tctcgagtgc
      541 cccagcagcg aggatgaagg ttctcgcgcg ggcaacgaat cggttgccgg aaagggttgc
      601 acagtcgatc cggtcgacgc ggcctcgact gtcgccgaga tgcacggcgt tggcgtgcag
      661 aacgagggtc atattctcgg cgtctacgag gtccggctcg tagcggtcga acctgctgtc
      721 cttgccgaac tggaagatct cggatcggac gacttccggg tcgagccgta agggagctcc
      781 gtagccttgt gcctgccagt cagcagtttc gtaggtgaat ggtcccaacg cgcagcgttc
      841 gtgggctcgc cgatagaacg ggtcgagatg ggatcgatcg aagggccagc catggtcggg
      901 gaatctgtcc cgggcctcga aatcgagaga gtccagcgga cgagcttgta gccctctgcc
      961 cgcgtgccag gcgcgcgacg agccgccgaa cgctcgcgcc ctcgtgaatc gaagcgggta
     1021 gtagacgtgc ccggcgttca cgccctttgc cagctcctgt tcgcccactg agtgcctgcg
     1081 aaccacggta ggcaaatcgc gaagggattt cgtgaatgaa agaccacctc cctcgaggag
     1141 cagaaccttc tccgggcggc cgatgaactc catcgccagc gagatcccgg caacacctgc
     1201 gccgacgatg cagatgtctg cctcgagcgt cgacccgtcg gcgatgtgtc tcccgtcaat
     1261 caacatatcg gtctccgtcg ccaaccctca ttttcgcaac cacgactcga gcatgagcac
     1321 ctgccacagg tggtcgaccc ggtcgcggcg accagagagg tgttcttccc acttcacacg
     1381 aatcggctcg ggatccagca ggccctcctg ccggattctg tccgcatcga ggaggctctc
     1441 cgcccagtcg cgcagcggtc ccctgagcca gctgcccaac ggcagatgga aacccatctt
     1501 ggggcgctgg tagagggtct cgggcacgta tcggtgcaag acttggcgga ggatgtactt
     1561 tccggtctcg ccccgcactt tcatctcggg aggtaaacgg aacgcgaact cgaccatgcg
     1621 atggtccatg aacggcaccc tggcctccac gctcgcggcc atgctcgctc ggtcgacctt
     1681 catgagctgg gtgtccacga aggtcgcggt cagctcatgc gccaggacac gctcgaggat
     1741 ctcgacgtcg ccgtcgatcg gagcgagttc gaactccatc ggatagcggt ccgggtcctc
     1801 ggcgacgatc aatcggtctt cggcatccca acgcgtcgac agcacccggt acatgtcctc
     1861 cggcgaagca tgtgtcatcc agcgggccaa ccggtggagc ttggccccgg gggtgcgctc
     1921 gcggaatctc ggcgggatca cgggctgcat cacgtcgaat gccttgtccc acgagcccgg
     1981 tggaactgcg gctattgctc gtgccgcctg ctccctggct cccacgggta gccatcccat
     2041 ccgctgccac actctgggga gccagcgatg gcggtggtat ccggcgaaca gctcgtcacc
     2101 accgtcgccg gcgagggtca cggtcactgt ctccccggcc agacggtagg cggcatgatt
     2161 ggagacgtaa gaggggtcag actgcggttg gtcggtcacc cgggccagcg tcgggatcag
     2221 gtcgatgatg tcctcagggg ccatgtagag ctcggtgtga tcggagccga tgtgagccgc
     2281 gatctcccgc gccgcgggtg cttcgtccag gcccgagtgc ggaaagccga tcgagaagct
     2341 gtggactctc ctgggactct ggtgttggag gagcgccagg acgctcgatg agtcgatgcc
     2401 ccccgatagc agcactccga gtgggacgtc gctgacggtc cgcagccgga cagagtctcc
     2461 cagcagttga tcgagcatgt cggcggcgtc tgccgcctca cccgtgaagc gatcccgcct
     2521 ccctgcctcg acggtctcga tcagcgacca gtagacgcgc tccttcgcct gcagagttgc
     2581 atcgaaggtg acaatggtgc cagggcggac ggcctggatc tgcgagtaga tcgtctcgtc
     2641 cgacctgatg tggatctcac gtaaatagcg gtcgagccaa tctcgattga tcgtgaggtc
     2701 ggcgttggga taggccgcta tggcgtgaag ctccgagccg aacaccatcg cgccattgac
     2761 gttgccaaag tagagaggct tgatgccaag ccggtcgcgg accaacgtga gggttcgatc
     2821 ccgtttgtcc catagagcga aggcgaacat gccgttgaaa gaggcgagcg cccgatcgat
//
LOCUS       DASUTA010000049         3868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1702209, whole genome shotgun sequence.
ACCESSION   DASUTA010000049 DASUTA010000000
VERSION     DASUTA010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702209"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="VFZ80_00955"
     CDS             complement(<1..1202)
                     /locus_tag="VFZ80_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186255.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX6286028.1"
                     /translation="MSGHHELANKVRFVTATSLFDGHDASTNIFRRILQASGAEVIHL
                     GHNRSGIDIVDAAIQEDAHAIAVTSYQGGHMEFYRYLADLLAERNASLIRVFGGGGGT
                     ILPSEGDELARQGVTRIYSPDDGREMGLSGMVADMLARSDYPRGELPADLDLSALTTW
                     RTDLVASLISAAENYPDKNADIWEKLEEQAAASPAPVVGITGTGGSGKSSLVDELVRR
                     FLIDFEDRRIAIISVDPSKRRTGGALLGDRIRMNSLPHPRAYMRSLATRQANLALSPH
                     VHHAIDIVKAAGYDLVVLETSGIGQSDTEIVDFSDHALYVMTPEYGAATQLEKIDMLD
                     FADMVAINKADKQGAEDALRDVRKQYKRNHLAFDAGDDELPIHLTVASDFNDPGTNRF
                     YRALMSRLG"
     gene            1248..1781
                     /locus_tag="VFZ80_00960"
     CDS             1248..1781
                     /locus_tag="VFZ80_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX6286029.1"
                     /translation="MTSTHQTVLDPIAEARRQWVAHGWGEAAPGMAVVTTITRVQQLL
                     EARVEDVLAPFGLTFARFEILRLLGFTRTGRLPMGKISDRLQIHPASVTSAVKRLERE
                     GLISRTPDADDNRVVLASLLPAGRRVLDPATAAVNEVFLSTGLQRSEIDRLIALLDPI
                     RSQAGDQVEMTPAAMDD"
     gene            1849..2157
                     /locus_tag="VFZ80_00965"
     CDS             1849..2157
                     /locus_tag="VFZ80_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286030.1"
                     /translation="MAQFTGRLQGPKLEPSAVLIDIGDGRFRVSAGRLQLGSWALERI
                     TAERTSIYKFTLDIDDEHFDFYPDDPSTFSEAIGAIIDLTESKGRFGLRRRIEQAANG
                     "
     gene            2189..2935
                     /locus_tag="VFZ80_00970"
     CDS             2189..2935
                     /locus_tag="VFZ80_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX6286031.1"
                     /translation="MARSLLKRGATFVLAVLGAEIAFAVLKRSPELESFDPSGEFGDP
                     DSPTLRVAVLGDSSVTAPGVAGPEEIWVSLVCSRLAENHHIVLKSFAVGGSMAHNLVA
                     EQMGDTIDFEPDLIFVSVGANDALRGIPVRVFEKNLDYLIRRLAQTGATIIQSGVGVM
                     GTSPRLHPPLSTMMSGQAIRFDRAHRRVAAKYGSWVVDHRSDDAGLWHQDASLWAADD
                     FHVSAAGHARWAETTWRTVGPLMSRSSAAS"
     gene            2922..3335
                     /locus_tag="VFZ80_00975"
     CDS             2922..3335
                     /locus_tag="VFZ80_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003942513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX6286032.1"
                     /translation="MRRAEALARAAFERSGHLATVRPDGRPHVVVVTFALVGGHAVTA
                     IDHKPKRTDRLQRLVNVEQTGRASLLVDHYEEDWSRLWWVRIDGSASLHEADELYSSA
                     IDGLVAKYAQYSDRAPEGPVIAISQDDVSSWESTP"
     gene            complement(3310..>3868)
                     /locus_tag="VFZ80_00980"
     CDS             complement(3310..>3868)
                     /locus_tag="VFZ80_00980"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX6286033.1"
                     /translation="ASGPMDKDSLRSLARSQAPVPGEVSEEVADGLFAWLSSRLPGTV
                     SAFLAMDGEVDVASLFGRLPGWRWVLPRVEPDRTMTFRDRDVPREVHRFGMEQPTDAG
                     PVIPVHEIDVFLTPGLAFDLRGGRVGNGAGFYDRILVKRRTDAQAVGVTVESRVFESV
                     PMDAHDQRVDWIATESGVRECFPKS"
BASE COUNT          680 a         1232 c         1236 g          720 t
ORIGIN      
        1 cccaggcggg acatgagggc gcggtagaac cgattggtcc ccgggtcgtt gaagtcagac
       61 gcaaccgtga ggtggatggg cagctcgtca tcccctgcgt cgaaggcgag gtgattgcgc
      121 ttgtattgct tgcgcacatc ccgcagcgca tcctcggctc cctgcttgtc agccttgttg
      181 atcgcaacca tgtcggcgaa gtcgagcatg tcgatcttct ccagctgcgt cgccgccccg
      241 tactcggggg tcatcacgta gagggcatgg tcggagaaat cgacgatctc ggtgtccgac
      301 tggccgatcc ccgacgtctc caggaccacg aggtcgtacc cggcggcctt gacgatgtct
      361 atggcgtggt ggacatgagg ggacagcgcg agattggcct gccgggtggc cagggatcgc
      421 atgtaggcac gggggtgggg cagcgagttc atccggatcc ggtcacccag gagggctcct
      481 ccggtgcgcc gcttggatgg atcgactgag atgatggcga tccgcctgtc ctcgaagtcg
      541 atgaggaatc gtcgaaccag ctcgtcgacc aggctcgact tgccggaacc gccggtcccg
      601 gtgattccga ccaccggcgc cggtgacgct gctgcctgct cctcgagctt ctcccagatg
      661 tccgcgttct tatcgggata gttctcggcc gctgagatga gactggcgac caggtcggtt
      721 ctccaggtcg tcagggccga cagatcgaga tcggcgggga gctctcctct cggatagtcc
      781 gatcgcgcca gcatgtccgc aaccatcccg ctgaggccca tctcccggcc atcgtccggc
      841 gagtagatcc tggtcacacc ctggcgcgcc agctcgtcac cttccgaggg gagaatggtc
      901 ccaccacccc caccgaagac ccggatgagg gaggcgttcc gctcggccaa caggtcggcg
      961 aggtagcggt agaactccat gtgcccgccc tggtaggagg tgacagcgat ggcgtgggcg
     1021 tcttcttgga tggcggcgtc cacgatgtcg atcccgcttc ggttgtgtcc gaggtggatc
     1081 acctcggcac cagaagcctg gaggatgcgc cggaagatgt ttgtcgacgc gtcgtgaccg
     1141 tcgaagaggg aggtcgcggt gacgaaccgg accttgttcg ccagttcatg atgtcccgac
     1201 attgtttgcc ttcaggatat tcgtgattta ttttgatgtc aaaggaaatg acctcgacac
     1261 accaaaccgt tctcgatccg atcgccgagg cacgccggca gtgggtggcc cacggttggg
     1321 gagaggccgc tccgggcatg gcggtcgtca ccacgatcac ccgtgtgcaa cagctactcg
     1381 aggctcgggt cgaagatgtc ctcgctccat tcggcctcac cttcgcccgc ttcgagattc
     1441 tccgtctctt gggtttcaca cgaaccgggc gcctcccgat gggaaagatc tccgatcggc
     1501 tccagatcca cccggccagc gtcaccagcg cggtcaagcg tctcgagcgg gagggactga
     1561 tcagtcgcac gcccgatgcc gatgacaacc gggtcgttct cgcgtcactg ctgcccgcgg
     1621 gtcgacgcgt gctcgatccg gcaaccgccg cggtcaacga ggttttccta tcgaccggcc
     1681 tccagcgcag cgagatcgat cgactcatcg ccctcctcga cccgatccgg tcccaggctg
     1741 gtgatcaggt cgagatgacg ccggcggcga tggatgacta gtcagtagtg gctcgctgtc
     1801 attagcactg ctcccctcgt ctcgaatcgg actagattgc acagtgggat ggctcaattc
     1861 acaggaaggc tgcagggacc caagctggag cccagcgctg tgctgatcga tatcggggac
     1921 ggtcggttcc gcgtgtccgc aggccgattg cagctcggct cgtgggcttt ggagagaatc
     1981 actgcagagc gaacctccat ctacaagttc acactcgaca tagacgacga gcatttcgac
     2041 ttctacccgg acgacccgtc cacgttttct gaggctattg gagcaatcat cgatctgacc
     2101 gagtcgaaag gccgtttcgg ccttcgccgg cggatcgagc aggcggccaa cgggtagccc
     2161 tgttcgcaac ggctggcaga atagggggat ggccaggagc ctgctgaaac gtggcgcgac
     2221 ctttgtgctc gccgtcctcg gcgccgagat cgcattcgcc gtcctcaaac ggtctcccga
     2281 gctggagtca ttcgatccgt ccggggagtt cggtgaccct gactccccca ccctgcgggt
     2341 tgcagtgctc ggcgactcct ccgtcaccgc acccggagtc gccggtcccg aggagatctg
     2401 ggtgtcactg gtgtgttccc gcctggcaga gaaccaccac atcgtgctga agtcgttcgc
     2461 cgtgggcggc tccatggccc acaacctcgt tgcggagcag atgggggaca ccatcgactt
     2521 cgagcccgac ctgatcttcg tgtcggtggg tgccaacgat gccctgcgag gcatcccggt
     2581 gcgcgtcttc gagaagaatc tcgactacct catccgccgg ctggcgcaaa ccggggcgac
     2641 catcatccag agcggtgtcg gcgtgatggg cacgagtccg cgcctccatc ctcctctgag
     2701 cacgatgatg tccgggcagg caatcagatt cgatcgcgca caccggaggg tggcggccaa
     2761 atacggcagc tgggtggtcg atcatcggtc cgacgacgcc ggtctctggc accaggacgc
     2821 ctctctctgg gcagccgacg actttcacgt cagcgcggcc ggccacgccc gttgggcgga
     2881 gacgacgtgg cgcaccgtgg gcccattgat gagtcgctcc agtgcggcga gctgaggcac
     2941 tggctcgagc tgccttcgag cgcagcggcc atctcgccac ggttcgaccg gacggaaggc
     3001 ctcacgtcgt ggtggtgacc ttcgctctgg tgggcggaca cgccgtcacc gccatcgacc
     3061 acaaacccaa gcgcaccgac cggttgcagc gactggtgaa cgtcgagcag acagggcggg
     3121 cctcgttgct cgtggatcac tacgaagaag attggagccg tctttggtgg gtgcgcatcg
     3181 acggatcggc ttcgttgcac gaagctgatg agctctactc gtcggcaatc gacggtctcg
     3241 tcgcaaaata tgcccagtat tcagatcggg caccggaggg gcctgtgatc gcgatctctc
     3301 aagacgacgt cagctcttgg gaaagcactc cctgacgccc gactcggtgg cgatccagtc
     3361 gacccgttgg tcgtgggcat ccatggggac cgattcgaag accctggact ccactgtgac
     3421 ccccacggcc tgggcgtcgg ttcgtcgctt gacgaggatc cggtcataga agcccgcacc
     3481 gtttccgacc cgccctccgc gcagatcgaa ggcgagcccg ggagtcagga agacgtcgat
     3541 ctcatggacc gggatcaccg gaccggcatc ggtcggttgc tccatcccga accgatgtac
     3601 ctcacggggg acatcccgat cccgaaaggt catggtgcga tcgggctcga cccggggcag
     3661 gacccatcgc cagccgggga gtcgcccgaa caaggaggcc acgtctactt caccgtccat
     3721 ggccaggaag gccgagaccg tccccggcaa acgcgaggag agccaggcaa acagaccatc
     3781 ggctacctct tcgctcacct cgccgggcac cggggcttgg gatctcgcca acgagcgcag
     3841 gctgtccttg tccattggtc cggaggct
//
LOCUS       DASUTA010000050         7945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1314641, whole genome shotgun sequence.
ACCESSION   DASUTA010000050 DASUTA010000000
VERSION     DASUTA010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314641"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFZ80_00985"
     CDS             <1..273
                     /locus_tag="VFZ80_00985"
                     /inference="COORDINATES: protein motif:HMM:NF025204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 family protein"
                     /protein_id="HEX6286034.1"
                     /translation="EDDEEHDEETAEALEELENQELQLLDEEANEAMLVDEVAELRAM
                     RREELTLNVDAQSQKADEFVCQSCFMVKRTSQLANKRKMICFDCVD"
     gene            439..1182
                     /locus_tag="VFZ80_00990"
     CDS             439..1182
                     /locus_tag="VFZ80_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX6286035.1"
                     /translation="MSDSGGEPNPERVVVALPTYNEAENLLEIVSLILAHGYRLLIID
                     DNSPDGTGAIADRLAEEDERVEVLHREQKAGLGPAYADGFDHALASGAEVVIEMDADL
                     SHDPADLPRLLSAVDGGADIAIGSRYVPGGETPDWPAIRQLVSKGGNLYARMMLSIPI
                     RDATAGFRAFRAEALRRLPYRSAEASGYGFQVEMAWRAHQAGLIVEEIPITFRDRTKG
                     TSKMGMSIVVEAMRLVTVWGVQRMVGRSS"
     gene            1179..1658
                     /locus_tag="VFZ80_00995"
     CDS             1179..1658
                     /locus_tag="VFZ80_00995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6286036.1"
                     /translation="MIVTARRADEADLDTLVGLYRGLEEEMEALHVMWPVADGLDEPI
                     SESFRAALEEPDTVVLIGEIDDYPFGFLLARVERLLAQADGELVGAIRLIYVDQEARE
                     VAVAEEMREMALDILRERGITKFDAHVLPGHRLAKNFFEAGGFSARSIIMHHDDEKE"
     gene            1645..2043
                     /locus_tag="VFZ80_01000"
     CDS             1645..2043
                     /locus_tag="VFZ80_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX6286037.1"
                     /translation="MRRSDLPVVGVGAVVFDDEGRILLIERGREPQKGLWAVPGGKVE
                     YGEEMREAVRREVAEETGLEIDVGDVVWVGEIIDAEFHLVLIDFEATVIGGQLQAADD
                     AADVRWVSLDDASELPLTPTMYDLVDTLLS"
     gene            2040..3368
                     /locus_tag="VFZ80_01005"
     CDS             2040..3368
                     /locus_tag="VFZ80_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6286038.1"
                     /translation="MTDEAVALLQQLIRNRCVNDGTADSGQEHRSVETLTEFFGVEGE
                     IFEPHPGRQSLMYRVAGTDPDAPSLGLAPHIDVVPVEVSGWTQDPFGAEIVDGFVYGR
                     GAVDMLNVTAAMAHAARPYIKGDIRARGDLLFVALADEEAGGALGAMPLTEQRWDDVE
                     VDYMLTEVAYPKMSYATEPAIPVATGEKGAFWSLLRATGTPGHGSAPFGSDNALRKMV
                     EAVHGIFAEPMPVVITHEWEAFVASLALDPDVKEALVDPDRVDDALETLAIDDPLFAR
                     YAHAVTHLTISPNVLEGGVKSNVIASQAKANIDIRALPGIDRSFVDSFLYKAMGSARD
                     DIDIEPIQDFEATQSPTGNPLWEAIADTVEELEGHRRLLPAIMNVATDARFWRKRGTI
                     AYGVGLFDDRMDFSEMLALFHGHDERVSVESVRRTTTMYHKVLARFLGSS"
     gene            3370..4140
                     /locus_tag="VFZ80_01010"
     CDS             3370..4140
                     /locus_tag="VFZ80_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX6286039.1"
                     /translation="MTVLCDLDGVIYRGRSVLPGVPDALTRLRDADIDIFYITNNSTR
                     TPAAGAEKVRALTGIDVDESQILTSSLAAVSVLEPEDGPVLVVGEAGVSDAVARAGLE
                     ETEDPGLARAVLVGLARHIDYDLLGRAMGAILSGARFIATNDDATFPTENGFMPGAGA
                     IVAAIAASTSTEPIVAGKPHPPMRQLIRSRGVGAAWVIGDRPDTDIAIAREEPDWRSI
                     LVLTGVAGDEDVGVSGADHVVADFPTAVDLVLSAPRKS"
     gene            4152..4295
                     /locus_tag="VFZ80_01015"
     CDS             4152..4295
                     /locus_tag="VFZ80_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286040.1"
                     /translation="MDLSPEQIEAIEEKLSQLSELDPAQLPGPAAELVELLNDILEET
                     ESG"
     gene            4292..5065
                     /locus_tag="VFZ80_01020"
     CDS             4292..5065
                     /locus_tag="VFZ80_01020"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX6286041.1"
                     /translation="MKFALHVRTGRSAITEFAERLTTEIEAVGGSVVEDGVSPDMVLA
                     VGGDGTMLGAVYQAIGWDVPVLGFNLGTLGFLTEAQPDEMETVVGRLLSGDYEVDERM
                     TIQASTDGEILDGVNDVVVEKIESTRLVHLEVVVDGTPFTTYRADGLIVATPTGSTAY
                     SFSAGGPIVDPGVAALVLTPVAAHSLFDRPIVLPADTEVTVTVHRDRPVRVNVDKSVL
                     AELGDGDRVNIRRGPRPARFVTFDTVSSPGLVRDKFGLD"
     gene            5065..6654
                     /locus_tag="VFZ80_01025"
     CDS             5065..6654
                     /locus_tag="VFZ80_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6286042.1"
                     /translation="MLDHLRVANLGVIEDAAIDPSSGFTVITGETGAGKTLLLGGLRL
                     ILGGQADSNAVGPYGAESTIDGFFTRGEEETGATRVVPADGRSRAYLEGSIVSASTLT
                     ERLGSMIEIVGQHDQLAITRPSHLLELIDGASEDMTTRDSYRDAWGRLQGALDKQRQL
                     GGDRIELTRELDLARYQASEIAAARLEPGLDEELEGSVSRLRNVEEIREHLSETMNLT
                     EQMSEVVGEMVARLRKASGLDPSLADLANEAEGIAAGVAELAREARGSEEGLDADPEA
                     LVALEEQLTDLGDLKRKYGRTLEEVIAYGEQTSRRADELEELIADADEIDSIVSRTRA
                     EVAKRAQALSKTREETAFGVCREVADHLSDLGLGSARVEIHLEEVGPGPSGADRPVLR
                     FASDSRLELGPVSSVASGGELSRLVLALRLATRSESTTTLVFDEVDTGIGGSTALAMG
                     RKLRALASAGQVLCVTHLAQVAAHADTHYVVERADSGVATVRKVTDDDRVTELSRMLA
                     GQPDSAAGQIAAAELLAIGAS"
     gene            6754..>7945
                     /locus_tag="VFZ80_01030"
     CDS             6754..>7945
                     /locus_tag="VFZ80_01030"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX6286043.1"
                     /translation="MNGPLFSPEQAPEEKAPSTDPTKYVFVTGGVVSSLGKGIAAASL
                     GRLLKSRGLRVVNQKLDPYINVDPGTMNPFQHGEVFVTDDGGETDLDLGHYERFTGEN
                     LRRTSNVTSGSVYLSVIQKERRGDYLGDTVQVIPHITNEIKERIRRVGDQDVDVVITE
                     VGGTVGDIESLPFLEAIRQFGNEAGRENVAFVHVTLVPYIATSEELKTKPTQHSVAEL
                     RSKGITPDVIIVRSDREIDEGVRKKISLFCDVEQRAVIAAPDVRDIYEVPLILHDGGL
                     DAVICDRLGLVTGPPNLESWRSMVAKAMSATDEVRIGIVGKYVGLPDAYLSVAEALRH
                     AAAGQGLRLDLVWVPSDELTGLLADSYLEDLEGIVIPGGFGYRGVEGKIQAIRFAREN
                     NVPLL"
BASE COUNT         1444 a         2465 c         2622 g         1414 t
ORIGIN      
        1 gaggacgacg aggagcacga cgaggagacg gcggaggccc tcgaagagct cgagaatcag
       61 gagctgcaac tcctcgatga agaggcgaac gaggccatgc tggtcgacga agttgccgag
      121 ctacgtgcga tgcgccggga agagctcacg ctcaacgtcg acgctcagag ccagaaggca
      181 gacgagttcg tctgccagtc ctgtttcatg gtgaaacgga ccagccagct ggcgaacaag
      241 cgcaagatga tctgcttcga ctgtgtggac tgatcgtttc gcatcagaaa cgctgacgca
      301 tttccgatgc gagccctgca cgaccggcct gcggccgcgc tcattcttgc tggctgaacc
      361 ggcgaagccg gctcagccag gtcgtggggg gattggcgca gccacccccc tcgagcaccc
      421 cccaactgtc agcgttgcgt gagcgattcc gggggagaac ccaatcctga gcgggtcgtc
      481 gtcgccctgc ctacgtacaa cgaagcggag aacctgctgg aaatcgtgtc gttgatactc
      541 gctcatggct atcggctgct gatcatcgac gacaactcac ccgacggcac gggtgccatc
      601 gccgaccgtc ttgcggagga ggacgagcgt gtcgaggttc tccatcgaga gcagaaagcc
      661 ggtctggggc ccgcttatgc cgacggcttc gaccacgccc tggcgagcgg cgccgaggtc
      721 gtcatcgaaa tggacgccga tctctcacac gaccctgcag acctgccacg cctgttgtcg
      781 gccgtcgatg gcggcgccga tatcgccatc ggctcccgtt atgtcccggg cggagagacc
      841 cctgattggc cggcgattcg ccagctggta tccaaagggg gcaacctcta tgcccgcatg
      901 atgctttcga tcccgatccg cgacgccacg gccgggttcc gggccttccg cgccgaggct
      961 ctgcgaagac tcccctatcg cagtgccgag gcctccgggt acggctttca agtcgagatg
     1021 gcctggcgtg cccaccaggc cggcctgatc gtcgaggaga ttccgatcac attcagagat
     1081 cgaaccaagg ggacctccaa gatgggcatg agcatcgttg tcgaggcgat gcgactggtg
     1141 acggtctggg gtgttcagcg catggtcggc cggtcgtctt gatcgtcacc gcacgccggg
     1201 ccgatgaggc cgacctcgac acgctggtcg gcctgtatcg cggtctcgaa gaggagatgg
     1261 aggcactcca cgtcatgtgg ccggtggcgg atgggctcga cgagccgatt tctgagtcct
     1321 tccgcgctgc tctcgaggag cctgacaccg tcgtgttgat cggggagatc gatgactatc
     1381 ccttcggctt cctcctggct cgggtcgagc gtctgctggc ccaggcggat ggtgagctcg
     1441 tcggtgcgat caggctgatc tatgtcgacc aggaagcacg tgaagtggcg gtcgccgagg
     1501 agatgaggga gatggcgctc gacatcctga gagagcgtgg cataaccaag ttcgacgccc
     1561 atgtgctccc cgggcacagg cttgccaaga acttcttcga agcgggcggc ttctcagctc
     1621 gctcgatcat catgcatcac gacgatgaga aggagtgatc tgccggtggt cggtgtgggc
     1681 gccgtcgtgt tcgacgacga gggtcggatc ctgctgatcg agcggggtcg cgaaccccaa
     1741 aagggcctct gggcggttcc tggtggcaag gtcgagtatg gcgaagaaat gagagaggcg
     1801 gtgcgtcggg aggttgccga ggagacaggg ctcgagatcg acgtcggcga tgtcgtttgg
     1861 gtgggagaga tcatcgacgc tgagttccat ctcgtgctca tcgacttcga ggccactgtc
     1921 atcggaggcc agttgcaggc ggctgacgac gccgccgatg ttcgctgggt atctctcgat
     1981 gacgcatcgg aacttcccct gaccccgacc atgtacgacc tcgtcgatac gctgctgtca
     2041 tgacagacga ggccgtcgct ctccttcaac agctcatccg caaccgatgc gtcaacgacg
     2101 gaacggcgga cagcggccag gagcatcgct ccgtcgagac gttgaccgag ttcttcggag
     2161 tcgaaggcga gatattcgaa ccacatcccg gtcgtcagtc tctcatgtat cgagtggccg
     2221 gaaccgatcc cgacgcccca tcgctggggt tggcccccca tatcgatgtg gtccccgtgg
     2281 aggtgtccgg ctggacccag gacccgtttg gtgccgagat cgtcgatggc ttcgtgtacg
     2341 gccggggagc ggtcgacatg ctcaatgtga ccgcggccat ggctcatgcg gcccgtccat
     2401 acatcaaagg agacattcga gcgaggggtg atttgttgtt cgtcgcgttg gccgacgagg
     2461 aagcaggagg cgcactcggt gccatgccgc tcaccgagca gcgctgggac gacgtcgagg
     2521 tcgattacat gctcaccgaa gtcgcttacc caaagatgtc atacgcgacc gagccggcga
     2581 tcccggtggc gacgggggag aagggggcgt tctggtcgct gcttcgagcc actggaaccc
     2641 ccgggcacgg atcggcaccg ttcggctcgg acaatgccct gcgaaaaatg gtcgaagccg
     2701 tccacggcat cttcgccgag ccgatgcccg tggtcatcac acatgagtgg gaagcgttcg
     2761 tggcgtcgct ggcgctcgac ccggatgtga aggaggccct ggtagacccc gatcgcgtcg
     2821 atgatgcatt ggagactctc gccatcgacg atccgctctt cgccagatat gcccatgccg
     2881 tcacccatct gacgatctcg ccgaatgttc tcgagggcgg ggtcaagtcg aacgtgatcg
     2941 ccagtcaggc gaaagccaac atcgacattc gagcacttcc gggcatcgac cggtcgttcg
     3001 tcgattcctt cctgtacaag gccatgggct cggcacgcga tgacatcgac atcgaaccga
     3061 tccaggactt cgaggcgact caaagcccta ctggcaatcc gttgtgggag gcgatagccg
     3121 acaccgtcga ggagctggag ggccaccggc gtctgctccc cgcgatcatg aacgtggcca
     3181 ccgacgcccg cttctggcga aagagaggga cgatcgcata tggtgtcggg ctcttcgacg
     3241 atcggatgga cttctccgag atgctcgcct tgttccacgg gcacgacgaa agggtctcgg
     3301 tcgagtcggt gagacgcacc accacgatgt accacaaggt tctggcccgt ttccttggct
     3361 cgagctgact tgaccgtcct ctgcgacctc gacggggtca tctaccgggg aaggtcggtt
     3421 ctccccggtg tccccgatgc gttgaccagg ttgcgcgacg ccgatatcga catcttctac
     3481 ataacgaaca actcgacacg cacgcccgcc gcgggcgcgg agaaggttcg ggccctcacc
     3541 gggatcgatg tcgacgagag tcagatcctg acgtcatccc tggccgctgt gagcgttctc
     3601 gaaccggagg acggcccagt gctggtcgtt ggtgaggccg gggtgagtga cgccgtggcc
     3661 cgcgccggtc tggaggagac agaagacccg gggctggcac gtgccgtcct cgtcgggctg
     3721 gccaggcaca tcgactacga cctcctggga cgcgccatgg gcgcgatctt gtcgggggcc
     3781 cggttcatcg cgaccaacga cgacgcaaca ttcccgaccg agaacgggtt catgcccggt
     3841 gcaggcgcga tcgtggccgc catcgcggca tcgacctcga ccgagcccat cgtcgctggc
     3901 aagcctcatc cgccgatgcg tcagttgatc cgatcacggg gtgtcggcgc ggcatgggtg
     3961 atcggggatc gacccgacac cgatattgcc atagcccgtg aggagcccga ctggcgctcg
     4021 atcctggtgc tgaccggtgt agctggggac gaggatgtgg gcgtttcggg tgcagaccac
     4081 gtcgtggccg actttcccac tgccgtagat ctagtccttt cggctccgcg gaagtcataa
     4141 tcgagagtgg aatggaccta tctccagaac agatcgaggc catcgaggag aagctgagcc
     4201 aactctccga gctcgacccc gcccaacttc ctgggccggc agccgagctt gtcgagctcc
     4261 tgaacgacat tctcgaagag accgagagcg gatgaagttc gcactgcacg tacgaacggg
     4321 ccggtcggcc atcactgagt tcgccgagcg cctcacgacg gagatcgaag ctgtgggtgg
     4381 atcggtggtc gaggatggtg tcagccccga catggtgctc gcagtaggtg gggacggaac
     4441 catgcttggt gccgtctatc aggctatcgg ctgggacgtc cccgtcctcg ggttcaatct
     4501 cggcactctc ggtttcctca cggaggccca acccgacgag atggagaccg tcgtgggacg
     4561 tctcctctcc ggcgactacg aagtcgacga acggatgacc attcaggcat cgaccgacgg
     4621 cgagatcctc gacggggtca acgacgttgt cgtcgagaag atcgagagca cgcgtctcgt
     4681 ccacctcgag gtcgtcgtcg acgggacacc attcaccacc taccgcgccg acgggttgat
     4741 cgtggcgacg cccaccgggt ctactgctta ctcgttctcc gccggtggtc cgatcgtcga
     4801 cccgggggtg gcggcgctgg tcctgacgcc ggtggcagcc cattcgctgt tcgatcgtcc
     4861 gatcgtgctc ccggccgata ccgaggtgac ggtcaccgtg catcgtgacc ggccggtgcg
     4921 tgtgaacgtg gacaagtccg tgctggctga gttgggggat ggcgaccgtg tcaacattcg
     4981 ccgcgggccc cgccccgccc ggttcgtcac gttcgacact gtgagctccc cggggttggt
     5041 cagggacaag ttcggtctcg actgatgctc gaccacctcc gtgtcgccaa cctcggggtg
     5101 atcgaggatg ccgccatcga cccttcgtcg gggttcacgg tcatcaccgg ggagaccggg
     5161 gcgggcaaga ccctcctgct gggcgggttg cgtctcatcc tcggcggcca ggccgattcg
     5221 aatgctgttg gcccttacgg ggccgagtcg acgatcgatg ggttcttcac gcgtggggag
     5281 gaagagaccg gcgccacccg cgtcgttccc gccgatggca ggagccgggc ctatctggaa
     5341 ggatcgatcg tctcggcgtc gactctgacg gagcgactcg gctcgatgat cgagatcgtg
     5401 ggtcagcacg atcagttggc catcacccgg ccctcgcatc tcctcgaact gatcgacgga
     5461 gcctccgagg acatgaccac ccgcgattca taccgggacg cctggggtcg gctccagggc
     5521 gccctcgaca aacagcgcca gctgggtggc gatcgcatcg aactcacccg agagctcgac
     5581 ctggcccgtt accaggcgtc cgagatcgct gccgcacggc tcgagcccgg gctcgatgag
     5641 gagctcgagg gctcggtatc ccggctgcgc aacgtggagg agatacggga gcacttgagc
     5701 gagacgatga atctcaccga gcagatgtcg gaggtggtcg gggagatggt cgctcggctg
     5761 aggaaagcct cgggactcga cccttcgctg gccgatctgg caaatgaggc cgagggcatc
     5821 gctgccggcg ttgccgagtt ggccagggag gcgaggggat cagaggaagg attggacgcc
     5881 gatccggagg ccctcgtcgc gttggaggag caactgacgg atttgggcga tctgaaacgg
     5941 aagtacgggc gaacgctcga ggaggtcatc gcatacggag agcaaacctc gaggcgggcc
     6001 gatgagctgg aggagctcat cgccgatgcc gacgagatcg attcgattgt ctcccgtacg
     6061 cgagccgagg tggcgaagcg agcacaggct ctcagcaaga cccgtgaaga gactgctttt
     6121 ggagtgtgtc gagaagtggc ggaccacctg agtgatctcg ggctcggttc ggcacgggtg
     6181 gagattcacc tggaggaggt ggggccgggc cccagtggcg ccgaccgccc cgtgcttcgc
     6241 ttcgcatccg actcccgtct cgagctgggc ccggtcagct cggtggcgtc agggggcgag
     6301 ctgtcccgcc tcgtcctggc acttcgtctc gccaccagga gcgagtcgac cacgaccctc
     6361 gtcttcgacg aggtcgacac cggcatcggc ggctcgaccg cgttggcgat gggcaggaag
     6421 ctccgcgccc tggcgtcagc tggccaggtc ctctgtgtca cccatctcgc ccaggtggcg
     6481 gcacacgccg acacccacta cgtggtcgaa cgcgccgact ccggggtggc cactgtgaga
     6541 aaggtcaccg acgacgaccg cgtcaccgag ctctcccgca tgctcgccgg ccaacccgac
     6601 agcgccgccg gccagatcgc ggccgccgaa ctgctcgcga ttggggcctc ctgaccgttc
     6661 tgtgaacggg agcggtcacc atgtgacgct ccacgttcac agaacggtat tgcaggggga
     6721 tctcctggag ccgtgctact ctggcgtccc gtcatgaacg ggccactttt ctcgccagag
     6781 caagctccag aagagaaggc cccatcgacc gaccccacca agtacgtctt cgtcaccggt
     6841 ggcgtggtct ccagtctcgg caaaggaatc gctgccgcgt cactgggacg gttgctgaag
     6901 tcgaggggtc ttcgcgtcgt caaccagaaa ctcgacccct acatcaacgt cgatccgggg
     6961 acgatgaatc ccttccaaca cggcgaagtg ttcgtcaccg acgacggtgg cgagaccgac
     7021 ctcgacctcg ggcactacga gcggttcacc ggcgagaacc tgcgccgaac ctcgaacgtg
     7081 acatcaggct cggtctatct ctccgtcatc cagaaagagc ggcgcggtga ctacctaggt
     7141 gacacggtcc aggtcatccc tcatatcacc aacgagatca aagagcggat ccggcgagtc
     7201 ggtgaccagg acgtggacgt cgtcatcacc gaggtcggtg ggacggtggg tgacatcgag
     7261 agcctcccct tcctcgaggc gatccgccag ttcggcaacg aagcggggcg ggagaacgtc
     7321 gccttcgtcc acgtcacgct ggtcccttac atcgccacct ccgaggagct caagaccaag
     7381 ccgacccagc attcggtcgc cgagttgcgg agcaagggga tcacccccga tgtgatcatc
     7441 gtgcgatccg atcgtgagat cgacgagggg gtgcgcaaga agatcagcct gttctgcgac
     7501 gtggagcagc gggctgtgat cgccgcaccc gacgtccgag acatctacga agtcccgctc
     7561 atcctccacg atggcgggct cgacgcggtc atatgcgacc ggctcggcct ggtcaccggt
     7621 ccgcccaatc tggagtcatg gcgctcgatg gtggccaagg ccatgtccgc caccgacgag
     7681 gtcaggatcg ggatcgtggg caagtacgtc gggctccccg atgcctatct ctcggtcgcc
     7741 gaggcgctcc gtcacgccgc cgcgggccag ggactgaggc tggacctggt ctgggtcccc
     7801 agcgacgagc tgaccgggtt gctggcagat tcgtatctcg aagacctcga agggatcgtc
     7861 atcccaggcg ggttcggata ccgcggggtg gaagggaaga tccaggcgat tcgcttcgcg
     7921 cgcgagaaca acgtcccctt gctcg
//
LOCUS       DASUTA010000051         2756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_407958, whole genome shotgun sequence.
ACCESSION   DASUTA010000051 DASUTA010000000
VERSION     DASUTA010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407958"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1131
                     /locus_tag="VFZ80_01035"
     CDS             85..1131
                     /locus_tag="VFZ80_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6286044.1"
                     /translation="MSIESKFADTDRLRVHYLHSGPDDGEPVVLIHGNLATGRFMEGI
                     LDDLPDRYRGIAPDMRGFGDTEPKAIDATRGLRDWADDTRALVETLGIDQPIHLVGWS
                     TGGAAIASYTVDSPHQVRSVTFIDPVSPYGFGSTKDTAGTSCFEDYAGSGGGGVVPEF
                     VERLSNKDDSDESPLSPRNVMNSSYWSSAHRESKEREDLLLAEIFKTSIGDGGYPGDM
                     ITSENWPGIAPGTTGILNALSGRYCRWDDIVDIEPKPPILWTHGTEDIVVSNQSAWDM
                     GVLGGAGLVPDWPGAEVFPPQPMVDQIRSVLEAYVANGGEVRMETIEGAGHGPHFDRR
                     EEWTAIFVDFLTSH"
     gene            1388..2626
                     /locus_tag="VFZ80_01040"
     CDS             1388..2626
                     /locus_tag="VFZ80_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX6286045.1"
                     /translation="MREAFFTLSTDWKEGINWANVRDWRLKRAHDAMERHGLGALLLF
                     YDENMRYVSSTLTPGWNRLKPGLRYVVLPRGRKPIVYEQGDIGFHLEVHNPWIPKENI
                     RYSYAWIKGAVGPAADQQVNKFVNALMNDLEDAGVRDEPLGVDFIDINLIRQFDEREI
                     TWTDGMSPMMEARAIKSPDEIKAFSIVGAICDYVHYEITNFIKPGLSENEVAAFGFEK
                     LYSIPGMEDVEDVIVSSGPNAWPNWRNFSDRMIRPGELVIIDLAALTWNGFKSCVYRT
                     YCVGGEPSEEHKQTYALAHKWLWDSIEATKPGVTTADVASKWPSAKEVWGYEEEDQAA
                     ANFWGHGLGLAQYDPPVISRIWSLDHPVEIEPGMTFALETQHGKLHDHGVRLEEMLYV
                     TETGIEMVSTYASHEIITVG"
BASE COUNT          545 a          857 c          848 g          506 t
ORIGIN      
        1 gtgacctgcg gggctgaagt ggtgggagag atggcacgcc tcttctggag tagtttctct
       61 cccctctgaa cgaggaggaa ggctttgtca atcgagtcga agttcgccga taccgatcgg
      121 ctgcgtgtcc actacttgca ctcgggtcct gatgacggcg agcctgtcgt cctcatccat
      181 ggcaacctcg ccacaggccg cttcatggag ggcatcctcg acgacctccc cgaccgctat
      241 cgaggcatcg cccccgacat gcgtgggttc ggtgacaccg agcccaaggc gatcgacgcg
      301 accaggggac tgcgggactg ggcggatgac acacgggcac tcgtcgaaac cctcggaatc
      361 gaccagccga tccatctcgt tggctggtcc accggtggcg ccgccatcgc cagctacacc
      421 gtcgacagtc cccaccaggt ccgctcggtc acattcatcg atcctgtgtc tccctacggt
      481 ttcggaagca ccaaggacac cgccggcacc tcgtgtttcg aggactatgc cggatcgggc
      541 ggcggtggag tggtccctga gttcgtcgag cgtctctcaa acaaggacga ctccgacgag
      601 tcgccgctct cgcctcgtaa cgtcatgaac agctcctatt ggtcctccgc tcatcgcgag
      661 tccaaggaac gcgaggacct cctcctcgcc gagatcttca agacctccat cggcgacggc
      721 gggtatccgg gcgacatgat cacatcggag aactggcccg ggatcgctcc cgggaccaca
      781 gggatcctca atgctctctc ggggagatat tgccgctggg acgacatcgt cgatatcgag
      841 cccaagccgc cgatcctgtg gacccatggc accgaggaca tcgtcgtctc caaccagtcg
      901 gcgtgggaca tgggggttct gggcggcgcc gggctcgtcc ccgactggcc cggtgccgag
      961 gtgttccctc cacagccgat ggtcgatcag atccgatccg tgctcgaagc ctatgtggcc
     1021 aatggtggcg aggtccgcat ggagacgatc gagggtgccg ggcacggccc ccacttcgat
     1081 cgtcgggagg agtggaccgc catcttcgtc gacttcttga cgagccactg actgcccacc
     1141 gccttgccga agttgcacct gccaccccgc tcgagggtcg aacgagtcat ccggataccg
     1201 ggcccttcgg ccgtataggg tgagaattgg tcagccggca ggaagaatgg atcgagtcga
     1261 tctttgccga gatcgactcg gagtcctcgg ccaagaaggg ccgtttcgac aatgacgagt
     1321 tctggacatg gtccggtggt gatcccagag agtctgggtt gagcgaacca gggagactgc
     1381 gatagcaatg agagaagcgt tcttcacgtt gagcaccgac tggaaggaag gcatcaactg
     1441 ggccaatgtc agagactggc ggctgaagcg tgcccacgac gccatggagc ggcacgggct
     1501 cggggcgctc ttgctgttct acgacgagaa tatgcgctac gtctcctcga ccctgactcc
     1561 agggtggaac cggctcaagc ccggtctgcg gtatgtggtg ttgcccaggg gcagaaagcc
     1621 catcgtctac gaacagggcg acatcggctt tcacctcgag gtacacaacc cctggatccc
     1681 caaagagaac atccgctact cctacgcgtg gatcaaaggg gcggtcggtc cggcggccga
     1741 ccaacaggtg aacaagttcg tcaacgcctt gatgaacgat ctcgaggatg ctggagtccg
     1801 ggacgagccc ctgggagtcg acttcatcga catcaacctc atcaggcagt tcgatgagag
     1861 agagatcacc tggaccgatg gcatgtcgcc gatgatggag gcccgtgcca tcaagagccc
     1921 ggacgagatc aaggcattct ccatcgtcgg cgccatctgc gactacgtcc actacgagat
     1981 caccaacttc atcaagccgg gtctgagcga gaacgaggtg gcggcgtttg gcttcgagaa
     2041 gctctactcg ataccgggga tggaggacgt cgaggacgtc atcgtctcct ccggtccgaa
     2101 cgcctggccc aactggcgca acttctcgga tcggatgatc cgccccggcg agttggtgat
     2161 catcgacttg gcggccctca catggaacgg cttcaagtct tgtgtgtacc gaacgtactg
     2221 cgtcggcggc gagcccagcg aagagcacaa gcagacctac gccctggccc acaagtggct
     2281 ctgggattcc atcgaggcga caaaacccgg ggtgaccacg gccgacgtcg cttccaagtg
     2341 gccgtcggcg aaggaagtct ggggctatga ggaagaagat caagcggcgg cgaatttctg
     2401 ggggcacggc ctcggcctgg cccagtacga ccccccggtc atctcccgga tctggtccct
     2461 cgatcacccc gtcgagatcg agcccgggat gactttcgct ctcgagaccc agcacggcaa
     2521 gctgcacgat catggtgttc gtctcgagga gatgttgtat gtgaccgaaa ccggcatcga
     2581 gatggtgtcc acttacgcca gccacgagat catcacggtc ggctaggggt gtcgctcgat
     2641 tgcctctggc gccgcgaaaa tcacggcgtc accacccgtt gactgaacaa cgactttctg
     2701 gcccgagccc aattatcgac ctggacgaca cagcggctct cgacctgtcg cgggtc
//
LOCUS       DASUTA010000052         2245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_148880, whole genome shotgun sequence.
ACCESSION   DASUTA010000052 DASUTA010000000
VERSION     DASUTA010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148880"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VFZ80_01045"
     CDS             complement(<1..477)
                     /locus_tag="VFZ80_01045"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX6286046.1"
                     /translation="MDRVQLGVMQNPVRGFLHGTAALVSVVGLVVLVVRAQGVANTAA
                     SAIYGSTLIAMYVTSALYHSVPWQPKWKAKLQSLDHTFIYALVAATFTPLVIGTGETI
                     WVVLGLAGVWALVALGFLREIVHGPARRFLLPLQFLAVAVTLAPLMSTLVAIDTAAA"
     gene            796..960
                     /locus_tag="VFZ80_01050"
     CDS             796..960
                     /locus_tag="VFZ80_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286047.1"
                     /translation="MEETDQERAERHERERLEDAERHERERKEAHERHERERREEQEQ
                     KEREDDADQA"
     gene            complement(951..1853)
                     /locus_tag="VFZ80_01055"
     CDS             complement(951..1853)
                     /locus_tag="VFZ80_01055"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: protein motif:HMM:NF015370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HEX6286048.1"
                     /translation="MAQSEKLALVEAIESEVDSLTQAHLTRRSDWYFHDIVPWERGES
                     FRDKPWDVSQCTISEDARTSLVLNLLTEDNLPYYHALIEKHMPSTSAFFRWNRLWTAE
                     EGQHSIAIRSYLLTSRNADPRQLEDDRKATMMNGYQPSYQDPTEIFAYTATQELATRV
                     NHRNAGKITDDPAAYELMRNIAADENHHFIFYKSVMQAMLDQAPGLVLEGIHRVFENF
                     QMPGVAMPNFLRRSIEVAKAGVYNLRIHHDRVLLPLIKDWNIENLTGLTPAAAELQEK
                     IMQLPAQVLRKAEVFEKRVGISYA"
BASE COUNT          434 a          627 c          734 g          450 t
ORIGIN      
        1 ggcggccgcg gtgtcgatgg cgaccagcgt gctcattagg ggtgccagtg tgacggcgac
       61 cgccaggaac tggaggggga gcaggaatct gcgggctgga ccgtgaacga tctcccgcag
      121 aaacccaagg gccacgaggg cccagactcc ggcaaggccg agaacgaccc aaatggtctc
      181 acccgtgccg ataaccagtg gtgtgaacgt ggctgccacg agcgcgtaga tgaatgtgtg
      241 gtcgaggctc tgcagctttg ccttccattt gggctgccag ggaacagagt gatagagggc
      301 actggtgacg tacatggcga tcagagtcga accgtagatg gcactcgctg cggtgttggc
      361 aacaccctgt gctcggacca ccaggaccac gagaccgacg acggacacaa gcgccgccgt
      421 gccgtggagg aacccacgaa ccgggttctg catcactccg agttgcaccc gatccatctg
      481 ctcacattac atccagccct ggacctgttt gtggggatgt ggtcgagcga taggatctcc
      541 ttgtggacca ctcgttcccg catcagggtc tggaagaagc aaccactccg accgccgcca
      601 ggttcggatc gatcgagtgg ccgtccacag aggatgtcga agctttcccg aacgcgggcg
      661 tgcagtcgtc aaatggggat ggtggaggag ctcagcaggg tctggctcaa gatcctcgat
      721 cacatcagac tgtgggggct tcggagacca ttgaatgatc gatggcctcg gactcgtaga
      781 aagttgagtc gttggatgga agagaccgat caggaaaggg ccgagcgcca cgagcgggag
      841 cgtctcgaag acgccgagcg tcatgagcgg gagcgcaagg aggcgcatga gcgccacgaa
      901 cgtgaacgcc gcgaggagca ggagcaaaag gagcgcgagg acgacgccga tcaggcgtag
      961 ctgatcccga ctcgcttctc gaagacttcg gctttgcgca agacctgggc cggcagctgc
     1021 atgatcttct cctgcaactc tgctgcggct ggggtgaggc ctgtgaggtt ctcgatgttc
     1081 cagtccttga tgagcggtag caagacccga tcgtggtgga tccggaggtt gtacacgccg
     1141 gctttggcta cttcgatcga tcgcctcagg aagttcggca tggcgacgcc cggcatctgg
     1201 aagttctcga acacgcggtg gattccctcg agaaccagac caggcgcttg atcgagcatg
     1261 gcttgcatca cactcttgta gaagatgaag tgatggttct cgtcggcggc gatgttgcgc
     1321 atcaattcgt acgctgccgg atcgtcggtg atcttgccgg cgttgcgatg gttgactcgg
     1381 gtagcgagct cctgggtggc ggtgtaggcg aagatctcgg tggggtcctg gtagctcggc
     1441 tgatagccgt tcatcatggt cgcctttcgg tcgtcctcca gttgacgagg atcggcgttg
     1501 cgcgaagtga gaaggtagct acgtatggcg atcgaatgct ggccttcctc ggccgtccag
     1561 agccggttcc acctgaagaa agcggacgtg cttggcatgt gcttctcgat gagagcgtgg
     1621 tagtaaggca ggttgtcttc ggtcagaagg ttgaggacca gcgaagtccg agcgtcctcg
     1681 gagatggtgc actgggaaac gtcccagggc ttgtcccgga agctctctcc cctttcccag
     1741 gggacgatgt cgtggaagta ccagtcggag cgccgggtga ggtgggcttg ggtgagactg
     1801 tcgacttcgg attcgatggc ctccaccagc gcgagcttct ctgattgggc catgagatcc
     1861 tgtcgttggt gatcctgacc ctagagcacc tgagtccgtt gtcccattgg agtctccgaa
     1921 ttcggaatga tcggtagccc ccgcctggtg ctagcttgag tcgagccgca ccgggtggag
     1981 gtcagcgacc tttcgctttc ccggtgatcg acggcacgtt cccagcccgg ttggactacc
     2041 tctttcggcc gaatcgcaga tgccacgttt cagggcatcc atccagattg ttcggcccga
     2101 ccttcttcga aggagaagag accatgtccg ccacgtttca agacttcgcg ctgcccgaga
     2161 agctcatcgc agctctcgag atccgcgacc tgaccaagcc tttccccatc cagctctcgg
     2221 ccatcccacc ggctttggcg ggaca
//
LOCUS       DASUTA010000053         2715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1185852, whole genome shotgun sequence.
ACCESSION   DASUTA010000053 DASUTA010000000
VERSION     DASUTA010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185852"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VFZ80_01060"
     CDS             complement(<1..374)
                     /locus_tag="VFZ80_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEX6286049.1"
                     /translation="MAMVLAMHAPDGFLNPVTALVTAIISVAVVALAMRHSRQQLADK
                     AIPLAGITAAFIFAAQMFNFPVAAGTTGHLLGGALAAVLLGPSVGVVVVTIVVVVQAL
                     VFADGGLTALGYNVLNMAIVPA"
     gene            456..878
                     /locus_tag="VFZ80_01065"
     CDS             456..878
                     /locus_tag="VFZ80_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX6286050.1"
                     /translation="MEAATDLGYLLRDHGYRLTSPRWLVWSVLRSADGHLTAEEIALA
                     VNRADPTVNISSIYRSLALFAELDLVRESHLGIDDSARWEIAHPDDHFHMVCRECGTV
                     DHHVGELVDQIRTHLGEQHSFSADNVDLVVTGVCDRCS"
     gene            992..1687
                     /locus_tag="VFZ80_01070"
     CDS             992..1687
                     /locus_tag="VFZ80_01070"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6286051.1"
                     /translation="MRIRELMTADVITIGPDASLKEAARRMIEAGVSGLIVTDDDGSL
                     VGVITEADFVKSESDRRPKKRARVLSWFSHDVEVPHFERLVGDAMTSEVVSVSPETDH
                     AEAARVMQTEKVKRLPVVDDGELIGIISRSDILRAFARPDESIIEELKEHVMRKVLWV
                     DPKRVEVHVVEGNVVLEGRLETKSDAILLEDLAGRLDGVVSVKSRLTWEVDNTKLEMV
                     SPPPGVPPHGNWA"
     gene            1696..2115
                     /locus_tag="VFZ80_01075"
     CDS             1696..2115
                     /locus_tag="VFZ80_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6286052.1"
                     /translation="MSDGPVVVGVDGSEQSIHAARLAADEARLREVELHVVHCVDIQP
                     AILHLPEGTTDTREIAKRRHDAVHDAVGEALSGISYERVGREGYAPDELIDYIDEVGA
                     SLLVLGSRGRGRVAGAVLGSTSLRCVEQAKIPVLIVK"
     gene            complement(2123..>2715)
                     /locus_tag="VFZ80_01080"
     CDS             complement(2123..>2715)
                     /locus_tag="VFZ80_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6286053.1"
                     /translation="GKFVAGISVIALMLTGLVMLVAGIGIFRLGVTPSVAEVSRLFVW
                     LLFAIVYVAFWQGLATLVSVRTNRAATSALIPVGIWLVLALFGSFIFSAVADVISPED
                     GTAAAALANAETEHLISQISPINLFQEGSTVLLDPEVRTTGLLTLEQVDRAIVSELNL
                     TQSLGVVWPQMVVLLAMTAGLFALAYISFMRQEVRA"
BASE COUNT          553 a          831 c          885 g          446 t
ORIGIN      
        1 taggcaggga cgattgccat gttgaggacg ttgtacccga gggcggtgag cccgccatcg
       61 gcgaaaacga gcgcctggac caccaccacg atcgtcacca ccaccacgcc gaccgaggga
      121 ccgagcagca cggcggcgag agcgccgccc aagagatgac cagtggtacc cgccgctacg
      181 gggaaattga acatctgggc ggcgaatatg aaggcagcgg tgatgcccgc aagcggaatc
      241 gccttgtcgg ctagttgttg tctcgagtgg cgcatcgcca gagcgaccac ggccacgctg
      301 atgatcgccg tcaccagcgc ggtgaccggg ttgagaaagc cgtcgggggc atgcatggcc
      361 aggaccattg ccatcacgtt acacgttgtt gcgatcgact cgcaacaagc atccactcgc
      421 agagagggag atctccaaat acgatgggag gaatcatgga agctgccacc gatctgggtt
      481 acctcctccg cgaccacggc tatcgactga ccagcccgcg ttggctggtt tggtcggtgc
      541 tccgctctgc cgacgggcat ctcaccgcag aggagatcgc actggcggtc aatcgggccg
      601 acccgacggt gaacatctcg tcgatctatc ggtctcttgc cctgttcgcg gagctcgatc
      661 tggtcaggga gtctcacctg ggcatagacg actcggcgcg ctgggagata gcccaccccg
      721 acgaccactt ccacatggtg tgccgggaat gtggcaccgt cgatcaccat gtcggggagc
      781 tggtcgacca gatcaggact cacctcggag agcagcactc gttctcggcg gataacgtcg
      841 acctcgtcgt caccggggta tgcgaccgct gcagctagcc ctgcggttcc gggccgttgg
      901 gcctgacgcc ttgtgaccta cggatctgtc cccggaatga ggactgcggg acgattggta
      961 cattcaaccc ttcgaaggag gattagaccc gatgagaatt cgtgagctca tgaccgcaga
     1021 cgtgatcacc atcggccccg acgcgtcact caaagaggca gccagacgga tgatcgaagc
     1081 cggtgtgagc ggtctgatcg tgaccgacga cgacggcagc ctggtgggcg tgatcaccga
     1141 ggccgacttc gtcaagagcg agtccgaccg ccgtcccaag aaacgagcac gtgtcttgag
     1201 ctggttcagt cacgacgtcg aggtacccca cttcgagcgt ctcgttgggg acgcgatgac
     1261 ttccgaagtc gtgagtgtct ccccggagac agaccacgcc gaggcggcgc gtgtgatgca
     1321 gaccgagaag gtcaagcgtc tccccgttgt cgatgatggc gagctgatcg ggatcatcag
     1381 ccgctcggac atactgcgcg cgttcgcccg acccgacgag agcatcatcg aggagctcaa
     1441 ggaacatgtc atgaggaagg ttctctgggt agacccgaag agagtcgaag tccacgtggt
     1501 cgagggcaat gtcgtcctcg agggtcgctt ggagacgaag agcgacgcga tcctgttgga
     1561 agacctcgcc ggtcgcctcg acggggttgt gtcggtgaag agccgtctca cctgggaggt
     1621 cgacaacacc aaactcgaga tggtctcgcc tccacccgga gtgccgccac acggcaactg
     1681 ggcataatgc ccgggatgag cgacgggccc gtcgtcgtcg gggtcgacgg gagtgagcaa
     1741 agcatccacg cggcgcgttt ggccgcggat gaggcccggc tccgggaggt cgagctccat
     1801 gtggtccact gcgtggacat ccaaccggcc atcctccact tgcccgaggg caccactgac
     1861 accagagaga tcgccaagcg gcggcatgac gcggtccacg atgccgtggg cgaagccctt
     1921 agcggcatct cgtacgagcg ggtaggccgg gagggttatg cccccgacga gttgatcgac
     1981 tacatcgacg aggtgggagc cagcctgctc gtcctgggat cgaggggccg gggccgggtg
     2041 gccggggcgg ttctcggctc gaccagtctc cgctgtgtgg aacaggccaa gatccccgtt
     2101 ctcatcgtga aatgagcgag gctcaggccc gcacttcttg acgcatgaat gagatgtagg
     2161 cgagcgcaaa cagacccgcc gtcatcgcca gcaacaccac catctgaggc cagaccacgc
     2221 ccaggctctg ggtcaggttc agctcggaga cgatcgcccg gtcgacttgt tccagcgtga
     2281 gcagaccggt ggtgcgcacc tctgggtcga gaagcaccgt ggacccctcc tggaataggt
     2341 tgatcggcga gatctgggag atgagatgct cggtctcggc gttggccagt gccgcggcgg
     2401 ccgttccgtc ctctggtgag atcacatcgg cgacagcgct gaagatgaag ctgccgaaca
     2461 gcgccagcac cagccagatg ccgaccggga tcaaggctga agtcgcggcc ctgttcgtcc
     2521 gaaccgagac gagagtcgcc aaaccctgcc agaaggccac gtagacgatg gcgaagagga
     2581 gccaaacgaa cagcctggag acctccgcca ccgagggcgt cactccgagc ctgaagatcc
     2641 cgatcccggc gacgagcatg accaggccgg tcagcatcaa ggcaatgacg ctgatccctg
     2701 ccacgaactt gccca
//
LOCUS       DASUTA010000054         2078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_538672, whole genome shotgun sequence.
ACCESSION   DASUTA010000054 DASUTA010000000
VERSION     DASUTA010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538672"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /gene="sufC"
                     /locus_tag="VFZ80_01085"
     CDS             <1..155
                     /gene="sufC"
                     /locus_tag="VFZ80_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010142576.1"
                     /note="part of SUF system involved in inserting
                     iron-sulfur clusters into proteins; in Escherichia coli
                     this protein forms a complex with SufBD; the SufBCD
                     complex stimulates the cysteine desulfurase SufS in
                     conjunction with SufE; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX6286054.1"
                     /translation="ITHYQRLLDYVTPDIVHIFMDGRVHESGGADLAEKLEGEGYESF
                     RTALTK"
     gene            161..1375
                     /locus_tag="VFZ80_01090"
     CDS             161..1375
                     /locus_tag="VFZ80_01090"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX6286055.1"
                     /translation="MNDLSHLKADFPVLDSGVHFLDSGASSQKPIPVLEAMDHFARTS
                     YANVHRGAYKLSVASTEAYEAARNRVATFINAATPEEVVFTRGATTALNTVAFGWGLE
                     NLSDGDRILATEMEHHANLVPWQMVAKRTGAKLDHVSITDDYRLDMDDYQSKIGPDVK
                     IVAVTGMSNVLGTMPDIPEIARIAHERGARVVVDAAQLVPHAGANVQTLGADFVAFSS
                     HKMLGSTGIGVLWGRMDALEETEPFEGGGEMIADVTLHDATWAEIPHRFEAGTPPIIE
                     AVGLHAAIDYLESIGMDRIFKHDQELTAMALERLSDVPGLSIQGPRELEDRGGVVSMV
                     MDGVHAHDLATILDEQGVAVRAGHHCAKPLMRRLGIAATARASFYVYNEEADIDALVT
                     GLRRASELFGVN"
     gene            1375..1824
                     /locus_tag="VFZ80_01095"
     CDS             1375..1824
                     /locus_tag="VFZ80_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX6286056.1"
                     /translation="MALEELYREVILDHYRNPRNRGRLDSPDATAQGVNPMCGDDITI
                     QLKFADGVVNEVAVDGQGCSISQSSASMMTEAIKGKTREEIEGLIAKFRTMMSLDDSE
                     DPGLDPDRPGSVLGDIEALQGVRKYPVRIKCASLGWNTLLEALNSTV"
     gene            complement(1859..>2078)
                     /locus_tag="VFZ80_01100"
     CDS             complement(1859..>2078)
                     /locus_tag="VFZ80_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286057.1"
                     /translation="ARAPDWTTRRSWRRLDTGDSAGIDCPVCGGVMKAPHVDGVMLDG
                     CPVHDVVWFAGPRSPDSAPGCSRRRAIG"
BASE COUNT          417 a          667 c          630 g          364 t
ORIGIN      
        1 tcatcaccca ttatcagcgg ctcctcgact acgtcacccc agatatcgtg cacatcttca
       61 tggatggccg agtccacgag tccggtggcg ccgatctcgc cgagaagctc gaaggcgagg
      121 gctacgagtc cttccggacg gcactcacga agtagacacg gtgaacgatc tctcccacct
      181 gaaggccgac ttcccggtcc tcgactcggg agtccacttc ctcgactcgg gggcgtcctc
      241 gcagaaaccg atcccggtcc tggaggcgat ggatcacttc gcccggacct cctacgccaa
      301 tgtccatcgc ggtgcctaca agctgtcggt ggcctcgacc gaggcctacg aagctgcccg
      361 caaccgtgtc gcgactttca tcaacgctgc cacgccggag gaggtcgtct tcaccagagg
      421 ggcgaccacc gctctcaaca cggttgcctt cgggtggggg ctggagaacc tgagcgatgg
      481 tgaccggatc ctcgccaccg agatggagca ccacgccaat ctcgtcccct ggcagatggt
      541 ggcgaaacga accggagcca aactcgacca cgtctcgatc accgatgact accggctcga
      601 catggacgac taccagtcga agatcgggcc cgacgtgaag atcgtcgctg tcactggcat
      661 gtcaaacgtc ctggggacga tgccggacat cccagagatc gccaggattg cacacgaacg
      721 tggagcccgg gtagtcgtgg acgcagctca gctcgttccc cacgcgggtg caaacgtgca
      781 gaccctcggc gccgacttcg tggccttctc atcgcacaag atgctaggct cgaccggcat
      841 cggtgttctc tgggggcgca tggatgccct cgaggagact gagccgttcg aagggggcgg
      901 ggaaatgatc gccgacgtca ccctgcacga cgcgacatgg gccgaaatcc ctcatcgctt
      961 cgaggcgggt accccgccga tcatcgaggc ggtcggcctg catgcagcca tcgattatct
     1021 cgagtcgatc ggcatggacc gcatcttcaa acacgaccag gagctaacgg cgatggccct
     1081 cgaacgtctc agtgacgtac ccggtttgag cattcagggc ccccgagaac tagaggacag
     1141 gggcggcgtc gtctcgatgg tcatggacgg cgttcacgcc catgacctgg caaccatctt
     1201 ggatgagcag ggagtcgcag tccgcgccgg acaccattgc gccaagccgt tgatgcgtcg
     1261 cctgggcatc gccgccaccg cccgtgccag cttctacgtg tacaacgagg aagccgacat
     1321 cgatgccctc gtcaccggac tacgccgggc cagcgaattg tttggagtga actgatggcc
     1381 ctcgaagagc tgtaccgaga ggtgatcctc gatcactacc gaaaccctcg taaccggggc
     1441 cgtctcgatt cccctgatgc caccgcccag ggtgtcaacc cgatgtgtgg cgacgacatc
     1501 accatccagc tgaagttcgc cgacggcgtc gtcaacgaag tagcagttga cggtcagggc
     1561 tgttccatca gccagtcttc ggcatcgatg atgaccgagg ccatcaaggg gaagacgagg
     1621 gaagagatcg aagggctgat cgccaagttc cggacgatga tgtctctcga cgattcggaa
     1681 gaccctggtc tcgacccgga ccgcccggga tcggtgctag gcgacataga ggccctacag
     1741 ggagttcgga agtatccggt gcggatcaag tgcgccagtc tgggatggaa cactctgctg
     1801 gaggcactga actcgactgt ttgacagtcg ggtcaaggta ggccgtcgag gaacctgatc
     1861 atccgattgc tctccgacgg ctgcagcccg gtgcggaatc aggtgatctc ggcccggcga
     1921 accacaccac atcgtggacc ggacacccat cgagcatcac accgtcgacg tgaggtgcct
     1981 tcatgacccc tccgcacacg gggcagtcga tgcctgccga gtccccggtg tcgagacggc
     2041 gccagcttct ccgcgtcgtc caatccggtg ctctggcg
//
LOCUS       DASUTA010000055         2741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1315971, whole genome shotgun sequence.
ACCESSION   DASUTA010000055 DASUTA010000000
VERSION     DASUTA010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315971"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VFZ80_01105"
     CDS             complement(<1..1002)
                     /locus_tag="VFZ80_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286058.1"
                     /translation="MANNIQVVEDGVGSSREQRPTQPSWIVGVAGFALGLGLGVLIVG
                     PTPAEPPIETTPGTASPDEGAAEVTTGTEVGDPSDDGVAGVIPGFPDAILVIARTSGT
                     ALDHVLWPNQGAVRVRSMTGGDEVRLDVGSQFIAMSDGVPGLDGVLLSMGRFNSIQPV
                     ASGVLGYSWHDSESALLAYTQSAGEVTRLSSVKADLTPVPVAEIPSPGGVIAGWGDWG
                     WAIQQRPNEIVLLAPGGEFKDSEPGVAHASHRSGWIFATEGPDAKFVSSGGGVRRVPE
                     PIGVGSVVDAAFSPDGSRVAVAGTRGLAVLDVETQEVTVLSESSTRSVAWSSDSRFVL
                     "
     gene            1146..2498
                     /locus_tag="VFZ80_01110"
     CDS             1146..2498
                     /locus_tag="VFZ80_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6286059.1"
                     /translation="MRLLAVLSVLVLIIAACGDGGGESTTTEADPGADTTAPTGTTEP
                     SSDTTEPMTDTTMAEGDLGSVTVATGDQIQIRSLNAISGDVAFLGTPNQVAVEMAIED
                     YGQIQGFDVTIGTGLDDLCSAEGGQAAAQTIAADEQVVGVIGTSCSGAAVAAMPVISN
                     AGLSMISPSNTSPALTSDLEGTAGTDYQPGYYRTAHNDLFQGQAVANFVFNDLGITTA
                     AAIHDGDPYTQGLAGAFQSAFEELGGTVTTFVGVNKGDTDMTPVLSEVATGAPEAIYF
                     PIFQPEGDFIVQQIGGVSGLEDATLIGADGLLVSDFLSLPETEGMYFSGPSLQFGDNV
                     NEITGVSATDFLVDYEAREGEAPSAAFWAHAYDATTMLLAAIDEVAVAGDDGLVIDRA
                     ALREAISASSFAGMIGNLACDDFGDCGSQEIQIVEHADSSVTDATQLPVVFTYSGLEG
                     "
BASE COUNT          512 a          896 c          860 g          473 t
ORIGIN      
        1 gaggacgaat cgggagtcgg acgaccacgc caccgaacgc gtcgatgact cgctgagcac
       61 tgtcacctct tgagtctcca cgtcgagaac ggccaggccg cgagtcccgg cgactgccac
      121 ccgcgagccg tcgggtgaga acgcggcgtc gacgacactg ccgacaccga tcggctccgg
      181 gacccgtcga acacctccac ccgatgagac gaacttggcg tcagggccct cggtggcgaa
      241 tatccagcca ctgcggtggg aggcatgggc gacaccgggc tcggagtcct tgaattcgcc
      301 tccgggggcg aggagcacga tctcgtttgg cctctgctgg atcgcccagc cccagtcgcc
      361 ccaaccagcg atgacgccgc cgggcgaggg gatctcggcg accgggaccg gggtcaggtc
      421 ggccttgacc gacgacaacc gcgtgacttc acctgccgac tgggtatagg cgaggagtgc
      481 gctctccgag tcgtgccagc tgtagccgag tacacccgat gcaaccggct ggatcgagtt
      541 gaaccggccc atcgacaaca agacgccgtc gagaccggga accccgtcgg acatcgcgat
      601 gaattgtgag ccgacgtcga ggcgaacctc gtcacctccc gtcatcgacc tcaccctcac
      661 agcgccctga ttgggccaga gcacatggtc gagagccgtg ccgctagtgc gtgcgatgac
      721 gagtatcgcg tcggggaacc ccggaatgac gccggcaacg ccgtcgtcgg acggatcgcc
      781 cacctcggtg cccgtggtga cctctgcggc gccctcatcc ggactcgccg tgccgggagt
      841 cgtctctatc gggggctcgg ccggggtcgg cccgacgatg aggacaccca gtcccaggcc
      901 gagcgcaaaa ccggccacgc cgacgatcca tgatggctga gttgggcgct gttcccggga
      961 ggaaccgact ccatcctcga cgacctggat gttgttcgcc atcgggtcac acggtacggg
     1021 aaagtggcga ttgggtgttc tctgcagtgc tcattgtgga aatggggtct aaagtcgtgc
     1081 ccacgacggg actttgctcc cgtcagctca attggaggaa ggaatccatg aaaactaaaa
     1141 ggagcatgcg acttctcgct gttctgagcg tcctcgtgct catcatcgcc gcctgtggtg
     1201 atggtggagg cgagtcgacg accacagagg ccgatccggg agcggacacg accgcgccga
     1261 ccggcacgac cgagccctcg tcggacacga ctgagccgat gaccgacacc accatggccg
     1321 aaggagacct cggctcggtg actgtcgcga ccggagacca gatccagatc cgctcgctca
     1381 acgcgatcag cggggatgtc gctttcctcg gcactcccaa ccaggtcgcg gtcgagatgg
     1441 ccatcgagga ctacggtcag atccaaggct tcgacgtgac gatcgggacc ggtctcgacg
     1501 acttgtgctc ggctgagggt ggacaggctg cggcccagac catcgccgcc gacgagcagg
     1561 ttgtcggcgt gatcggcaca tcgtgctccg gagctgctgt cgccgctatg ccggtcataa
     1621 gcaacgccgg cctgtcgatg atctctccgt ccaacacgtc tccggctttg acctcggatc
     1681 tcgagggcac cgctggaacg gactaccagc cgggttacta ccggaccgca cacaacgacc
     1741 tgttccaggg tcaggcagtg gccaacttcg tgttcaacga tctcggcatc accactgcag
     1801 cagcgatcca cgacggtgac ccctacaccc agggtctcgc cggcgccttc cagagcgcct
     1861 tcgaggagct cggtgggacg gtgacaacgt tcgtcggtgt gaacaagggc gacacggaca
     1921 tgaccccggt cctctccgag gttgccaccg gggccccgga ggccatctac ttcccgatct
     1981 tccaaccgga aggtgatttc atcgtccagc agatcggcgg ggtatcgggt ctcgaggacg
     2041 ccacgctcat cggcgccgac ggtctcctcg tctccgactt cctgtcgctg cccgagaccg
     2101 aggggatgta cttctcgggt ccgagccttc agttcggtga caacgtcaac gaaatcaccg
     2161 gcgtctcggc cacggacttc ctggtcgatt acgaggctcg cgagggtgaa gcaccgtccg
     2221 ccgcgttctg ggcccatgcc tacgacgcaa cgacaatgct gctcgcagcc atcgacgaag
     2281 tcgccgttgc cggagacgat ggtctagtca tcgaccgggc cgctctccgt gaggcgatca
     2341 gtgccagctc gtttgccggg atgatcggca acctggcatg cgacgacttc ggtgattgtg
     2401 gatctcagga gatccagatc gtcgagcacg ccgactcttc ggtgaccgac gcaacacagc
     2461 tcccggttgt gttcacatac tcggggctcg aaggctgagc aggaacatct gaccgcgaac
     2521 aacacgaggg acccggcact ttcgagtgcc gggtccctct ctttttggta taaccctccc
     2581 tcacgcatgc atgggcagac atgacgacaa ctgacgaacg gcctcccgcg atcgaagaaa
     2641 agcgcaagcg ggagcggaag gctctccgtc tcacgtgggt cgatctcttc ctgtggggca
     2701 tccgtgtcgc ggcgatcatc gtcatcctct ggggcctcat c
//
LOCUS       DASUTA010000056         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1575048, whole genome shotgun sequence.
ACCESSION   DASUTA010000056 DASUTA010000000
VERSION     DASUTA010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575048"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..874)
                     /gene="map"
                     /locus_tag="VFZ80_01115"
     CDS             complement(71..874)
                     /gene="map"
                     /locus_tag="VFZ80_01115"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX6286060.1"
                     /translation="MITIKSRREFAKMQKAGAAVAAVHKAVRESARPGVELLELEDIG
                     ARIIAEHGCTSSFLNYHGTYPATLCLSPNDVIVHGIPDRYRLREGDILAVDAGAIFEG
                     FHGDAAFTMGIGEISPEAQHLIETTEEGMWAGIRHVHKGARLGDIGSAIHEIGDVNGY
                     GVVEEYVGHGIGREMHEEPQVPNYGTAGSGLKLKTGMALCIEPMYNLGGRETKLDPDG
                     WTVRTRDGSLSAHWEHTVAITPDGPQVFTAGSEAIPVEEAMHSLARNTS"
     gene            complement(871..1422)
                     /locus_tag="VFZ80_01120"
     CDS             complement(871..1422)
                     /locus_tag="VFZ80_01120"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002517589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX6286061.1"
                     /translation="MRLLFIGPPGAGKGTQAGKVAGRLGIPHISTGDMFRDHVARRTE
                     LGKKVEAIMAAGDYVPDEVTVAMLSDRIAQDDAERGFILDGFPRTEAQVASLDRLLGD
                     EGLDTVVVFDVDEDELTERLISRGRADDNEETIRNRFKIYLEQTQPLIDVYDARGLTV
                     SVDGIGEVEEVTERIVDEIDRRS"
     gene            complement(1526..>2119)
                     /locus_tag="VFZ80_01125"
     CDS             complement(1526..>2119)
                     /locus_tag="VFZ80_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HEX6286062.1"
                     /translation="IPIQFAKRVRGRRVMGGQSTYIPLKVNTAGVIPVIFATSVLLIP
                     SLIITAIPSDAAWLQTVFRWIDDNLGAGAQAVSWWYILILFALIVFFTYFYTAIQFDP
                     EQQAEMIQRQGGFIPGVRPGRQTSKHLAHILNRITLPGSIFLATISILPAIAGAVWNI
                     QIAFSGVSILIVVGVALETMKQIESQLTMRNYEGILT"
BASE COUNT          362 a          664 c          689 g          404 t
ORIGIN      
        1 cgtggtcttg ctcaagtcga ccaagtcgag cctgttcggg cgcgggaggt caggcgtccg
       61 gaagcgtgca tcaggacgtg ttcctggcga ggctgtgcat cgcttcttcc accgggatcg
      121 cctcggaacc ggcggtgaag acctgaggcc cgtcgggggt gatggcgacc gtgtgctccc
      181 aatgggccga gagcgatccg tctctggtgc ggacggtcca accgtcggga tcgagcttgg
      241 tctcacgacc gccgaggttg tacatcggct cgatgcacag agccataccg gtcttgagct
      301 tgagaccact cccggcggtg ccgtaattcg ggacctgggg ctcttcgtgc atctcccggc
      361 cgatcccatg accgacgtac tcctcgacca ctccgtaccc gttgacatcg ccgatctcgt
      421 ggatcgccga gccgatgtct ccgagacgtg cgcccttgtg aacgtgtcgg atccccgccc
      481 acatgccttc ttcggtcgtc tcgatgagat gctgggcttc cggcgagatc tcgccgatcc
      541 ccatcgtgaa cgcggcatcg ccgtggaatc cctcgaagat ggccccggca tcgacggcga
      601 ggatgtcccc ctcccgcagc cggtacctgt cagggatccc gtggactatc acgtcgttgg
      661 gtgacaggca gagggtggcc ggataggtcc cgtgatagtt gaggaacgag gaggtacagc
      721 cgtgctcggc gatgatccgg gcaccgatgt cctccaactc gaggagttcc actccgggcc
      781 gggccgactc acgtacggct ttgtggaccg cggccacggc ggccccggcc ttctgcatct
      841 tggcgaactc acggcggctc ttgatggtga tcatgagcgt cggtcgatct cgtccacgat
      901 cctctccgtc acctcttcga cctcgccgat tccgtcgaca gagaccgtga ggccgcgggc
      961 gtcgtagaca tcgatgagcg gctgggtctg ctcgaggtag atcttgaaac ggttgcggat
     1021 ggtctcctcg ttgtcgtcgg cgcggccacg ggagatgagc cgctcggtca gctcgtcctc
     1081 gtcgacatcg aagacgacga cagtgtccag gccctcatca ccgagcaacc tgtccagtga
     1141 ggcgacctgg gcctcggtgc gggggaaccc gtcgaggatg aacccgcgtt cggcgtcgtc
     1201 ctgggcgatc cggtcgctga gcatggcaac cgtgacttcg tcgggcacgt agtcgcccgc
     1261 cgccatgatc gcctcgactt tcttgcccag ttcggtccgt cgtgcgacgt gatcgcggaa
     1321 catgtcccct gtggagatgt gcggtatccc caggcgaccc gccaccttcc ccgcctgcgt
     1381 tcccttcccg gctcccggcg gcccgatgaa gagcaacctc atgatgaaac ctcggcgctg
     1441 tgcctgcggg ccttcccgtt ttcgcgcatc aagtgcgctg aaaacgcgcc atctgatgcg
     1501 cgaaaacggg gtgcggccgc cgtcatcacg tgaggattcc ctcgtagttg cgcatcgtca
     1561 gctggctctc gatctgcttc atggtctcca gagcaacacc aaccacgatg aggatggaaa
     1621 ccccggagaa ggcgatctgg atgttccaca ccgccccggc gatggcaggg aggatcgaga
     1681 tcgtggccag gaagatcgag cccgggaggg tgatcctgtt gaggatgtgt gccagatgct
     1741 tcgaggtctg ccggccaggc cgcaccccgg ggatgaaacc gccctgacgt tggatcatct
     1801 ccgcctgctg ctcgggatcg aactggatcg ccgtgtagaa gtaagtgaag aagacgatca
     1861 gggcaaagag gatcaggatg taccaccagc tcaccgcctg tgcgcccgct cccaggttgt
     1921 cgtcgatcca tctgaagacc gtctggagcc aggcggcgtc cgacgggatc gcggtgatga
     1981 tcagggaagg gatgagcagc accgaggtgg caaagatcac agggatgaca ccggcggtgt
     2041 tgaccttcag ggggatgtag gtggattgac cgcccatcac ccggcggccc cggacccgct
     2101 tggcgaactg gatggggat
//
LOCUS       DASUTA010000057         2517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_280183, whole genome shotgun sequence.
ACCESSION   DASUTA010000057 DASUTA010000000
VERSION     DASUTA010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280183"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VFZ80_01130"
     CDS             <1..639
                     /locus_tag="VFZ80_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013731892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286063.1"
                     /translation="GPNGAGKTTLALHLNGILRARRGEIRVGGLEMTEENLAEIRRRV
                     GLVFQDSNDQLFMPTVREDVAFGPANLGLRGEELEARVVGALGAVDGLDFADRPPHHL
                     SGGEKRRAALATVLAMEPEVLVFDEPSSGLDPAGRRELIEMLRRFPMTQILITHDMAL
                     ALELCTRAVIVSDGRVVASGETGEILRDADLLARYRLEFPHAPSPGMPLHPG"
     gene            1080..>2517
                     /locus_tag="VFZ80_01135"
     CDS             1080..>2517
                     /locus_tag="VFZ80_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX6286064.1"
                     /translation="MTAEPTRSEIAARAHAVDVDEVASALGSDLQSGLSPDEAQHRLE
                     IFGPNQIGEDPGPSRWGLLARQFTDVLVLILLVAALISGVLLGDWLEAAVIVAIVLIN
                     AAIGFVQEVKAADAAEGLRRLASPTATVVRAGSESRAPTESIVPGDVIMIEAGDRVFA
                     DARVAEASRLQVDESELTGESTPVFKGPAPVDENAALGDRTSLLFAGTIVVSGHGRAV
                     VVATGEETEIGRIAGMLREEEPPTPLERELTHVGRLLGVLAGGVAVLVFVLGALQGRP
                     LESMFLLAVALAVAAIPEGLPAVVGVTLGLGVQRMARLNAIVRRMPAVEALGAVTVIC
                     TDKTGTLTRNEIRVQEVGFEGQTVRDLANRADDPRVRRYAQVAVLCNDARSSADGWAG
                     DPTEIALLRSIEGFTDLGEVRSTWPRIDEIPFDSSRKRMSTLHRHDGRYVGMVKGAPE
                     SVVTDCSRVLTESGPSHLGDCDSQRWLAL"
BASE COUNT          428 a          779 c          857 g          453 t
ORIGIN      
        1 ggccccaacg gagccggcaa gaccactctg gccctccatc tcaatgggat cctcagagcg
       61 cgaagaggtg agattcgggt tggcggcctg gagatgaccg aggagaacct ggccgagatc
      121 cgccgtcggg tcgggttggt cttccaggac tcgaacgatc agctcttcat gccgactgtc
      181 cgcgaggacg tcgcctttgg ccctgccaat ctcggactca gaggggaaga gctggaagcc
      241 agggtggtgg gcgccctcgg agcggtcgac gggctggatt ttgccgatcg gccgccacac
      301 catttgagcg gtggcgagaa gaggcgggcc gccctggcca cggtgcttgc gatggagccc
      361 gaagtgctgg tgttcgatga gccgtcatcg ggattagacc ccgcaggccg tagggagctc
      421 atcgagatgc tgcgtcgctt tccgatgact cagatcctga tcactcatga catggcgctg
      481 gccctcgagt tgtgcacgag ggcagtgatc gtgagcgatg gcagggtggt ggcctcgggt
      541 gagaccgggg agatcctcag ggatgccgat ctgctggccc ggtaccgcct ggagtttccc
      601 cacgcgccca gtccggggat gccccttcac ccaggctgag cttcgaggac agcggctcga
      661 gacgtgttgg gctcggccga gtgaccgcgg ccccagagag gagggtcgcc gccagggcta
      721 ccacaccggc ctcgaggacg agggtccggg ccgagggatc gcacagcccc tgatcctcct
      781 cccgctcggg ttgtgccccg aacacgagca cggcgaacgg tgtcgggcac tcgacttcga
      841 ccctcaccag accgcctgag tcagttgttc gccaggcgcc ggtccaggac tcgtaggtcg
      901 tgaaggccag ggagtaaacc agggccgctg caatgacgag gacgacccaa ccggtccacc
      961 gagtcatgat ccgaagggta ttgccgtccc atcgcaacac ggcagagagc agagccgtct
     1021 ggatctccat acgaccacgg ccactggcag tatcacgctg tgctttcttc cgttgaccga
     1081 tgaccgcaga gcccacccgg tcagagatcg ctgcgcgggc tcatgccgtc gatgtcgacg
     1141 aggtggcatc ggcacttggc tccgatctcc agtccggtct gtcacccgac gaggcgcagc
     1201 atcgtctcga gatctttggc cccaatcaga tcggtgagga cccggggccg agccgctggg
     1261 gccttctggc gagacagttc acggatgtac tcgtcctgat actcctcgtc gctgctctca
     1321 tcagtggtgt tctgttgggg gactggctcg aagccgcagt gatcgttgcc attgtcttga
     1381 tcaacgccgc catcggcttc gtccaggagg tcaaggccgc ggacgccgcc gagggattga
     1441 gacgactcgc ctctcccacg gcaacagtcg tcagagcggg gagtgagtcg agggcaccta
     1501 ccgaatcgat cgtgcccggc gatgtgatca tgatcgaggc gggcgaccgg gtgtttgccg
     1561 acgcccgggt cgcagaagct tcgcggttac aggtcgacga gtcggagctc accggagaat
     1621 cgacgccggt tttcaaagga cccgcaccgg tggacgagaa cgccgctctc ggtgatcgca
     1681 cttcactcct gttcgccggg accatcgtcg tgtccgggca cggtcgggcg gtcgtcgtcg
     1741 ccacaggcga ggagaccgag atcggccgga ttgcgggcat gctgcgcgag gaagagccac
     1801 ccacccctct ggaacgtgag ttgacgcatg tcggacgtct tctcggggtg ctggcgggtg
     1861 gggtggcggt cctggtgttc gtgctgggag cactgcaagg acggccactc gagtcgatgt
     1921 tcctgctcgc ggtggccctg gccgtcgccg ccatccccga gggtcttcct gccgtcgtgg
     1981 gagtcacact cggcctggga gtgcagcgca tggcgcgcct caacgccatc gtgcgccgca
     2041 tgcccgctgt ggaggctctt ggtgcagtga cggtcatctg caccgacaag acaggcactc
     2101 tcactcgaaa cgagataagg gtccaggaag tggggttcga ggggcaaacg gtgcgtgatc
     2161 tggcgaatcg cgccgacgac ccgagggtac gccgctatgc ccaggttgcg gttctctgca
     2221 acgatgcccg ttccagtgcc gacgggtggg cgggggatcc caccgagatc gcactgctcc
     2281 gctccatcga gggattcact gatctcggcg aggtccgctc gacgtggccc cgcatcgacg
     2341 agatcccgtt cgactcctct cgtaagcgca tgtcgacgct tcatcgccac gatgggagat
     2401 atgtggggat ggtcaaaggc gcccccgaga gcgtcgtcac cgactgttcg agggtgctca
     2461 ccgagtcggg accgagccat ctcggggact gcgattctca gcgatggctt gctctcg
//
LOCUS       DASUTA010000058         2186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1187250, whole genome shotgun sequence.
ACCESSION   DASUTA010000058 DASUTA010000000
VERSION     DASUTA010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187250"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VFZ80_01140"
     CDS             <1..272
                     /locus_tag="VFZ80_01140"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6286065.1"
                     /translation="KATLGWGSEAVSEIERLNRELEKLTVPTLVIHGGEDRLIPTWCT
                     ESLGDVSCVDRKVYEGLRHETLNEPEGPEVVADIVSWLEEKLADL"
     gene            complement(269..1297)
                     /gene="tdh"
                     /locus_tag="VFZ80_01145"
     CDS             complement(269..1297)
                     /gene="tdh"
                     /locus_tag="VFZ80_01145"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010581715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEX6286066.1"
                     /translation="MRALVKHADEKGLRLEDVPVPEAGPDEVLIKVHTTGICGTDLHI
                     YEWDDWARSTIPTPMTVGHEFSGYIEALGSNVSGFVEGELVGAEGHVICGSCRNCLAG
                     RRHLCHQPRGLGVNIPGAFADYVVVPKTNVWRHLEDIDPEIAAIFDPFGNAVHTALKF
                     PVFGEDVLITGAGPIGLMATAVVRHAGARNVVVTDVNTYRLELAQVMGASRVLDISTG
                     SLEVVKEELGIIEGFDVALEMSGNPAAMNDILDNTIHGANIALLGLPAEPYAIDWSVV
                     IFNMLNIKGIYGREMYDTWYKMNVLIQSGLDISPVITHRFAAAEFDAAFEAIKSTESG
                     KVMLRWWD"
     gene            1360..1884
                     /locus_tag="VFZ80_01150"
     CDS             1360..1884
                     /locus_tag="VFZ80_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX6286067.1"
                     /translation="MDSLAGNLLVATPILLDPHFYRTVVLLIQHDDDGCVGIVLNRPT
                     DQPVSAHIPEWAERVPDPSIINYGGPVEPDVAIGLSLTSTGMPTGVPGLSLIDLGEAP
                     DDNVSPVRIYSGYSGWGADQLETEIATGSWYVVQASPDDPFDSDEGQWRRVLKRQPGF
                     LSVVSTFPDDVAMN"
     gene            1928..>2186
                     /locus_tag="VFZ80_01155"
     CDS             1928..>2186
                     /locus_tag="VFZ80_01155"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX6286068.1"
                     /translation="MITATGVMDNEQVYRSYSEELTRYATGLVGPFDASDVVIDACLR
                     AFETRSWPDVLDRRAYLYRTVLSVANDHHRRTLSRRLRELRT"
BASE COUNT          418 a          671 c          677 g          420 t
ORIGIN      
        1 aaaaagcgac attgggttgg ggttccgagg cggtctcgga gatcgagcgg ctcaatcgcg
       61 agctcgagaa gctcaccgtg cccacgctgg tgatccatgg tggcgaggac cggctcattc
      121 ccacctggtg caccgagtca ctgggcgatg tctcctgtgt cgaccgtaag gtctatgaag
      181 ggctccgtca cgagactctc aacgagcctg aggggcccga ggtcgtggcc gacatcgtct
      241 cctggctcga ggagaagctg gcagacctct agtcccacca gcgaagcatc accttgccgg
      301 actcggtgct cttgatggct tcgaaggcgg cgtcgaattc agcagccgca aagcggtggg
      361 tgatcaccgg ggagatgtcc agacccgact ggatgaggac gttcatcttg taccacgtgt
      421 cgtacatctc tctcccgtag atgcccttga tgttgagcat gttgaagatg accaccgacc
      481 agtcgattgc atagggctca gcaggtaggc ccagcaatgc gatgttggct ccgtgaatgg
      541 tgttgtccag gatgtcgttc atcgccgccg ggtttcccga catctccagg gcaacgtcga
      601 agccctcgat gatgcccagc tcctctttga ccacctccag actgccagtg cttatgtcga
      661 ggactctcga ggcacccatc acctgggcca gctcgaggcg gtaggtgttc acgtcggtga
      721 cgacgacatt gcgagcgccg gcatgacgga ccactgcagt cgccatcaaa ccgatcgggc
      781 cggcaccagt gatgagcaca tcctcaccga agaccgggaa cttgagggcg gtgtgcacgg
      841 cattgccgaa agggtcgaag atcgccgcga tctcgggatc gatgtcctcg aggtgacgcc
      901 agacgttcgt cttggggacg acgacgtaat cggcgaaagc gccggggatg ttcaccccga
      961 gtccgcgggg ttggtggcaa aggtgacggc gaccggcgag gcagttgcgg caggagccgc
     1021 agatcacgtg gccttctgct ccgacgagct cgccctcgac gaaccccgag acattggaac
     1081 cgagggcttc tatgtagccg gagaactcgt gtcctacggt catcggagtt gggatcgtcg
     1141 accgggccca gtcgtcccat tcgtagatgt ggaggtcggt gccgcatata ccggtggtgt
     1201 ggacctttat gagtacctca tccgggcccg cttcggggac gggcacgtcc tccagccgca
     1261 ggcccttctc atcggcatgc ttgaccaggg ctcgcattcc cgaacacgct agtgctgagc
     1321 cagaccgcta ccaggctcgg ttgtgccacg attggggcca tggattccct tgcgggcaac
     1381 ctcctggtgg caacacccat tctcctcgac cctcacttct atcggacagt cgttctcctc
     1441 atccagcacg acgacgacgg atgcgtcgga atcgtcctca accgcccgac ggatcagccg
     1501 gtgtcggccc acataccgga atgggccgag cgggtgcccg acccctcgat catcaactat
     1561 ggcgggccgg tagaaccgga tgtggcaatc ggcctctctc taaccagcac cgggatgcca
     1621 accggagtgc ctggtctgag cctgatcgac ctgggcgagg ccccggacga caatgtctca
     1681 ccggtgcgca tctactcggg gtactcgggc tggggagccg atcaactgga gaccgagatc
     1741 gcaaccgggt cctggtacgt ggtgcaggca tcaccagacg atcccttcga ctccgacgag
     1801 gggcaatgga gacgggtcct caaacgccaa ccgggctttc tatcggtcgt atcgaccttc
     1861 cctgacgacg tggcgatgaa ctgacatcgt tgtcacccaa gaccgggttc tgttccgtct
     1921 caagtgcatg atcaccgcca caggcgtgat ggacaacgaa caggtctata ggagctacag
     1981 cgaagagctg acgcgatacg ccacgggtct cgtgggtccg ttcgacgctt ctgacgtggt
     2041 aatcgacgcg tgtctcaggg ctttcgagac tcgctcctgg cccgacgtcc tcgaccgccg
     2101 ggcgtatctg taccgaaccg tgctgtcggt ggccaacgac caccatcgcc ggaccctgtc
     2161 gcgtcggctt cgagaattga gaacag
//
LOCUS       DASUTA010000059         2543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_539864, whole genome shotgun sequence.
ACCESSION   DASUTA010000059 DASUTA010000000
VERSION     DASUTA010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539864"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VFZ80_01160"
     CDS             complement(<1..1001)
                     /locus_tag="VFZ80_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6286069.1"
                     /translation="MSEREGRIEELVAELLTEHPPERTPDVEFWGAQYDKGLAWAWHP
                     EGFGGLGMSRRDQTRIDQLLEAGGASTENRYLNLLGIAMGAGVLDAHATDEQKNRWLR
                     PMFTTEEIWCQLFSEPGAGSDVAGLSTRAVKDGDEWVVNGQKVWTTLAHIAKWGMIVT
                     RTDPDAPKHQGMTYFIVDMEAPGVEVRPLYQITGEAEFNEVFFNDVRVPDANRIDAVG
                     AGWRVALTTLMNERVAIGGASAPRGHGYIGQAVDVWKETGEDDPVLRDELLHLWAEAE
                     AARLTSIRANQMASAGIPGPEGSTGKLAWANLNKEITSFTVELLGLDGTLYPEGYEL"
     gene            complement(1346..>2543)
                     /locus_tag="VFZ80_01165"
     CDS             complement(1346..>2543)
                     /locus_tag="VFZ80_01165"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6286070.1"
                     /translation="DDADLFRRRLSRGFGGDLDDDEDARARFDALFDKDRTFGVFDGN
                     DIVGTGGAFSLGVTVPGGAVVPMGGTTVISVQPTHRRRGILRDLMAKHLDEVAERGEP
                     LAGLWASESSIYGRFGYGQATYRHKIDLDANHVRFRDEELKGQVQLLEREDAQATVTG
                     LYRDAVSRRSGMLTRSDAWWKYRVLADVESWRGGKSALRYAIYEVDGEASGYALYRQK
                     SNWEDFVADGEVDVVEIIAEDGEAYRGIWSYLTHIDLFPRVSWWNSPVDDPLPLMITD
                     SRRVRRSVADALWIRLMDVPAALTARAYERDGVVTIEVNDNFGAGASGVYRLEVVDGE
                     SSCELVDTAPDVGLDLDVLGHLYLGGGNAETMAIAGRIGGETGAVQRLHAMFRTTSSP
                     WCPYVF"
BASE COUNT          469 a          849 c          746 g          479 t
ORIGIN      
        1 ctcaactcgt acccctccgg atacagagtc ccgtccagcc cgagcagttc gactgtgaaa
       61 gaggtgatct ccttgttcag attggcccag gccaacttgc ccgtggagcc ttccgggccg
      121 gggatgcccg ccgaggccat ctgattggct ctgatcgagg tgagacgggc ggcttctgcc
      181 tccgcccaca gatggaggag ctcgtcgcgc agcacggggt cgtcttcgcc ggtctccttc
      241 caaacgtcaa ccgcctgtcc gatgtagcca tgaccccggg gggcgctcgc cccaccgatg
      301 gcgacacgct cgttcatcag ggtggtgagg gcgacccgcc agccggcgcc gacggcgtcg
      361 atgcggttgg cgtcgggcac tctcacgtcg ttgaagaaga cttcgttgaa ctcggcctca
      421 ccggtgatct gatacagggg ccgaacctcg acaccgggcg cttccatgtc gacgatgaag
      481 taagtcatac cctggtgctt gggggcgtct ggatcggtgc gggtgacgat catgccccac
      541 ttggcgatgt gcgccagcgt cgtccacacc ttctgaccgt tgaccaccca ctcgtcgcca
      601 tccttgactg cacgggtgga gagaccggcc acatcggaac cggctcctgg ctcgctgaag
      661 agctggcacc agatctcctc ggtggtgaac atcggccgca accagcggtt cttctgctcg
      721 tcggtggcat gggcgtcgag aacgcccgct cccattgcga tccccaggag attgagatag
      781 cggttctccg tcgatgcacc gcccgcctcc agcagctgat ctattcgggt ctggtctctg
      841 cggctcatgc ccaagccacc gaatccctcc gggtgccagg cccaggccag acccttgtcg
      901 tattgggccc cccaaaactc gacgtcaggg gtcctctccg ggggatgttc ggtgaggagc
      961 tcggcgacga gctcctcgat gcgaccttca cgctcgctca ctatttgacc tttcgctcaa
     1021 actcgacccc acggtacccg tgactctgct cgggcgctcc ggctgcggtg tgtaagagtc
     1081 gtaaaacgtt tgactcatta ccgacagagg gctatcctgt aaagagcaat gagtgaatag
     1141 ctcattactg tgacccgagg agaagacgat gttcgcccct tactgcaacc gccacggatc
     1201 gagagtcctt ctgcccacct ctgctatcac ggcccttgtt cgacggaccg acggcctgga
     1261 agccgagttt ctctgccact gcggtgccca tggcgtctgg agagccgacg agctgatcac
     1321 agcgggatcg ccgcgctgat cccggtcaga acacgtaagg gcaccagggc gacgacgtcg
     1381 tcctgaacat ggcatggagc ctctgcacag ctccagtttc accaccgatt cggccggcaa
     1441 tcgccatcgt ctcggcgttg ccgcccccta aatacagatg ccccagcacg tcgaggtcga
     1501 gcccgacatc cggggcggtg tcgaccagct cacagctcga ctcgccgtcg acgacctcga
     1561 gccgatagac accgctggca ccggctccga agttgtcgtt gacttcgatg gtgacgaccc
     1621 catcccgctc gtaggctcgt gcggtgagcg ccgccgggac atccatgaga cgaatccaca
     1681 aagcatcggc aaccgacctg cggacccggc gtgagtcggt gatcatcagg ggcagagggt
     1741 cgtccaccgg ggagttccac caactcacgc gggggaagag gtcgatatgg gtgagatatg
     1801 accatatgcc tcgatatgcc tcgccatcct cggcgatgat ctcgacaaca tcgacctcac
     1861 catcggccac gaagtcctcc caattggact tctgccggta aagggcgtac cccgaggcct
     1921 caccgtccac ctcgtatatc gcgtaccgga gagcggactt gccgccacgc catgactcga
     1981 catcggccag gacccggtac ttccaccatg cgtcagaacg ggtgagcatc ccggatcgcc
     2041 tcgacaccgc gtctctgtag agaccggtga cagtggcctg cgcatcctcc ctttccagca
     2101 gctgcacctg ccccttcaac tcttcgtcgc gaaacctcac gtggttggca tctagatcga
     2161 tcttgtgtcg gtacgtggcc tggccgtatc cgaatctgcc atagatgctg ctctcggatg
     2221 cccacaaacc agcgagcggc tcgccccgct cggcgacctc gtcgagatgc ttcgccatca
     2281 gatcacgcag gatgccccgt cggcggtgag tgggctggac ggagatcact gtggtgccgc
     2341 ccattggcac gactgcaccg ccgggcaccg tcactccgag ggaaaaggcc cctccggtgc
     2401 ccacgatgtc attgccgtcg aaaacgccga aggtgcgatc cttatcgaaa agggcatcga
     2461 aacgcgccct ggcgtcttca tcgtcgtcga ggtcaccacc gaagccacgg gagagccgtc
     2521 tccgaaagag atcggcgtcg tcg
//
LOCUS       DASUTA010000060         2738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1576078, whole genome shotgun sequence.
ACCESSION   DASUTA010000060 DASUTA010000000
VERSION     DASUTA010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576078"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFZ80_01170"
     CDS             <1..271
                     /locus_tag="VFZ80_01170"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX6286071.1"
                     /translation="PTGPLLYLDPDSVYDEVEFSLGKGDTLVAFSDGVADVQVAKNGR
                     TEPEVLADHLLAEGGNAARTADLVLGFAAPEPTDDQTVVVIRRVD"
     gene            333..707
                     /locus_tag="VFZ80_01175"
     CDS             333..707
                     /locus_tag="VFZ80_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286072.1"
                     /translation="MAEIHDFDDTVVAILDQEPAVHDAVEGLVAAGYEVEVLRGEEGK
                     EHLDPAGESGTIATVKRLLNVFGDQHRIIERLYRELDAGNMVVSVDAKPEEADEAIRI
                     LQDHGGHFVWKLGAWTFARIGE"
     gene            773..1123
                     /locus_tag="VFZ80_01180"
     CDS             773..1123
                     /locus_tag="VFZ80_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6286073.1"
                     /translation="MTGLTIRNSELAGGWQCIAVAGEIDLATVPELESAIDSAFSTDS
                     LSLVVDLRESSFMDSTGLKALVMANRKFIDDGRAFAIAVSGGPVSRLIDLSGVETSIR
                     IVDSTKDLEAETAD"
     gene            complement(1128..1484)
                     /locus_tag="VFZ80_01185"
     CDS             complement(1128..1484)
                     /locus_tag="VFZ80_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6286074.1"
                     /translation="MTLQVGSDVSAPSISRSRLRSMRAALGPRYDDVVLVVSELVSNS
                     VRHGMTNGIDVKVSTTPNTIRIEVTDDGPGFSIDDPRGEGMGLSIVEKLAEAWGMRDG
                     QQKFTVWAELSTHPSD"
     gene            complement(1548..2657)
                     /locus_tag="VFZ80_01190"
     CDS             complement(1548..2657)
                     /locus_tag="VFZ80_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6286075.1"
                     /translation="MTEVTQLTLPAEPASAAAARAFLHEHATLDGMRHAEADLLVTEL
                     IGNVLEHSPKSSEIVVGAQQDRDRGLVVSVSHPHPQPLEDSNRGVGFMLLERKTRAWG
                     HDHDGEMLTVWFVLRTPGTTSIPAEIPDSDLFSRMAEDPASYSDELVRRHTDLAASIA
                     RRYRGKGVDEDDLLQVANMALLKAIQRYDPSIGSIRPYAAATISGELKRLLRDKGWSV
                     RVPRSLQERSLEVAKTAELLAQKLNRPPETHEIAEQLELTEEEVVEALDARQAYASSS
                     IDKPADNTGVTLLERLEDDDVQLLLADDRVVVGEAVSQLPERQQRILDLRFNEDLTQT
                     EIAEELGISQMHVSRLLASALESLKKSIGKEGESD"
BASE COUNT          489 a          828 c          890 g          531 t
ORIGIN      
        1 ccccaccggg ccactcctct acctcgatcc cgactcggtc tatgacgagg tggagttcag
       61 tctcggcaaa ggtgacacac tcgtggcgtt cagtgacggg gttgccgacg tccaggtggc
      121 gaagaacggg cggaccgagc ccgaggtgct tgccgatcat ctgctggcag agggaggcaa
      181 tgcggcgcgc acagccgacc tcgtgctcgg ttttgccgcc ccggagccca cggacgacca
      241 gacggtcgtc gtcatcagac gagtcgactg attgtttgcc gacccgcgag cccgggtacc
      301 gattgcagct gttgttgcaa gggggaaccg aattggccga gatacacgat ttcgatgaca
      361 ccgtggtggc gatactcgac caggagccgg cggttcatga cgccgtcgag ggtctggtcg
      421 cggcgggata cgaggtggag gtacttcgtg gcgaagaggg caaggaacac ctggatccgg
      481 cgggagagtc ggggaccatt gccaccgtga agcgcctcct caatgtcttc ggagaccagc
      541 accggatcat cgagcgtctc tatcgagagc tcgatgccgg aaacatggtg gtgtcggtgg
      601 acgccaagcc cgaagaggcg gacgaggcga tccgaatcct ccaggatcac ggcggacact
      661 tcgtgtggaa gctgggcgca tggaccttcg cccgtatcgg tgaatgacga cgggtggagt
      721 ggtgaaggtc acagtgggtg tgccaacggc accctgatac gatctcatgc caatgacggg
      781 cttgaccatc cgcaactcgg agctggccgg cggctggcaa tgcatcgcgg tcgcagggga
      841 gatagacctg gccacggtgc cggagctgga gtcggcgatc gattcggcct tttccaccga
      901 ctccttgtcc ctggtcgtcg atttgcggga gtcgtctttc atggactcga ccgggctcaa
      961 ggctctcgtc atggcgaatc ggaagttcat cgacgatggc cgggctttcg ccatcgcggt
     1021 tagcggtgga ccggtctccc gactcatcga cttgagcggt gtggaaacgt cgatccgaat
     1081 cgttgatagc accaaggatc tcgaagccga gacagctgac tagcgactca gtcggaggga
     1141 tgggtggaga gctcagccca aacggtgaac ttctgctgac cgtctctcat gccccaggct
     1201 tcggcgagtt tctcgactat ggacagaccc atcccctcgc cacgggggtc gtcgatggag
     1261 aatcccggtc cgtcgtcggt gacttcgatg cggatcgtgt tgggggtggt actcaccttg
     1321 acgtcgatcc cgttggtcat cccatggcgg acgctgttgc tcaccaactc ggacaccacg
     1381 agaacgacgt cgtcgtatcg gggaccgagc gccgccctca tcgaccggag ccgcgagcgc
     1441 gatatgctcg gtgcgctgac atccgagcca acctgcaacg tcatctcatc catctctacc
     1501 gatcgccttc atggcgatac ccctctccaa gcaagagtaa acgggcccta atccgattcc
     1561 ccctccttgc cgattgactt cttgaggctc tcgagagcgc tggcgaggag ccgggacaca
     1621 tgcatctgcg agattcccaa ctcctcggca atctccgtct gggtcaggtc ctcgttgaat
     1681 cggaggtcga ggatccgctg ctggcgttcg ggaagctgac tcacggcctc cccgactacg
     1741 acccggtcgt cggccaacag cagctggaca tcgtcgtcct ccagtcgctc gagcagggtg
     1801 accccggtgt tgtcggctgg cttgtcgatg gagctcgatg catacgcctg gcgagcgtcc
     1861 agggcctcga cgacttcttc ttcggtcagt tcgagctgtt cggcgatctc gtgagtctcc
     1921 ggtgggcggt tgagcttctg agccaggagc tcagcggtct tggccacctc gagtgagcgc
     1981 tcctgcaacg atcgcgggac ccggaccgac cagcccttgt cccggaggag acgcttcaac
     2041 tcccctgaga tcgtcgcggc ggcgtatggc cggatcgacc cgatcgaggg gtcgtatctc
     2101 tgaatcgcct tgagcagagc catgttggcc acctggagca ggtcgtcctc gtcgactcct
     2161 ttgccccgat agcgtcttgc gatcgatgcc gccagatcgg tgtgcctccg caccagctcg
     2221 tcggagtagg aggcggggtc ctcggccatc ctcgagaaga gatccgagtc gggaatctcg
     2281 gccgggatac tggtggtccc cggcgtccgt aacacgaacc aaacggtgag catctcgccg
     2341 tcgtggtcgt ggccccaagc cctggtcttt cgttcgagca acatgaaccc gacacctcga
     2401 ttggagtcct cgagcggctg gggatggggg tgggagacac tgaccaccaa accccgatcc
     2461 cgatcctgtt gagcacccac gacgatctcg gagctcttcg gggagtgttc gagcacattg
     2521 ccgatcagct cggtcacgag gagatccgcc tcggcatgtc gcataccgtc gagggtggcg
     2581 tgctcatgga ggaaagcccg tgcggccgcg gccgaagcgg gctcggcggg gagcgtcaac
     2641 tgggtgacct ctgtcatggc ggagactcta tggattgtcg ccggcatgct catgtcgctg
     2701 agagtgggat cgggggcatc gctcaagcgc ggtcatcc
//
LOCUS       DASUTA010000061         2666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_540653, whole genome shotgun sequence.
ACCESSION   DASUTA010000061 DASUTA010000000
VERSION     DASUTA010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540653"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2060)
                     /gene="acnA"
                     /locus_tag="VFZ80_01195"
     CDS             complement(<1..2060)
                     /gene="acnA"
                     /locus_tag="VFZ80_01195"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX6286076.1"
                     /translation="MSNDPFGARATLETPLGAREIARLDVIEGVESLPYSIKVLLESA
                     LRNLNEISVTEADVREIAAYDATNVKESEIPFIPGRVILQDFTGVPAVVDLAAMRAAI
                     VRMTSDEGSAQKVNPLIPADLVIDHSVQVDAFARPDALIINSTKEFERNQERYEFLKW
                     GQSAFANFRVVPPATGIVHQVNLEYLAKVVWDDGEHLYPDSLVGTDSHTTMINGLGVV
                     GWGVGGIEAEAAMVGQPIYMLLPEVVGFRLTGKLPEGATATDLVLRVTEMLREHGVVG
                     KFVEFYGPGLADMPLANRATIANMAPEYGATVGYFPVDEQTLRYLRLTGREDKLIDTV
                     EQYYKEQGMWRDDSHAISYSADIELDMATVEPSLAGPRRPQDRIALSAMKEQWHSDLT
                     NTFGKNGGSATSTVTSWEAEGGTADHALEAQSSALIEYDGKTFTMKDGHVVIAAITSC
                     TNTSNPDVMVAAGLVARKARELGLQRQPWVKSSLAPGSKVVTEYLTESGLLNDLEDLG
                     FGIVGYGCTTCIGNSGPLPDPISEGIHAHDLVAASVLSGNRNFEGRISPDVRANYLAS
                     PPLVVAYALAGSVDWDPINEPIGKDRDGKEVFLEDIWPTQAEIASIVGVNVRQSQFLE
                     RYSDVFTGSEEWRAIATSGSDLYDWNPESTYVQEPPFFTDLTPDVRPIEPIVGARVLA
                     KLGDS"
     gene            complement(2298..>2666)
                     /locus_tag="VFZ80_01200"
     CDS             complement(2298..>2666)
                     /locus_tag="VFZ80_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286077.1"
                     /translation="NLSEIAEKVDRAYGQELASEIGAPHDPGYDEAVQAVASAMTGLG
                     FSVDPDIDGQRLLTSHCPFGETATSHPDVICSLDRGIVAGLFGALSVPCNPVVIPHQK
                     LDDDCVTQVPVTVGSRTSGS"
BASE COUNT          462 a          809 c          873 g          522 t
ORIGIN      
        1 gagtcaccca gcttggcgag cacacgggcg ccgacgatgg gctcgatggg acggacatcg
       61 ggagtcagat cggtgaagaa cgggggctcc tggacatatg tcgactccgg gttccagtcg
      121 tagaggtcgg agccgctggt agcgatggcg cgccattcct cagagcccgt gaacacgtcg
      181 ctgtatctct ccaggaactg gctctggcgg acgttcacac cgacgatcga cgcaatctcc
      241 gcctgggtcg gccagatatc ctcgagaaag acctccttgc catctctgtc tttgccgatc
      301 ggctcgttga tcgggtccca gtcgaccgat ccggccaggg cgtatgccac caccagcggg
      361 ggcgacgcga gatagttggc gcggacgtct ggtgagatcc gcccctcgaa gttccggttt
      421 ccactcaaca ccgaggccgc gaccaggtca tgtgcgtgga tgccctctga gatagggtcg
      481 ggaagggggc ccgagttccc gatgcatgtc gtgcatccat aacccacgat gccgaacccg
      541 aggtcttcta gatcgttcaa gagcccggac tcggtcagat actcggtgac cactttggag
      601 ccgggtgcca gtgagctctt cacccacggc tgacgctgca gtcccagctc tcgagccttc
      661 ctggcgacga gaccggcggc caccatcacg tcggggttgg aggtgttggt gcacgatgtg
      721 atcgcggcga tcactacgtg gccatccttc atggtgaagg ttttgccgtc gtactcgatg
      781 agggcggagc tctgcgcttc gagtgcgtgg tctgctgtgc ccccctccgc ctcccagctc
      841 gtcaccgtcg aagtcgccga accaccgttc ttgccgaagg tgttggtgag atcggagtgc
      901 cactgctcct tcatcgccga gagcgcgatc cggtcctgtg ggcgtctcgg tccggccagg
      961 gacggctcga cggtcgccat gtccagttcg atgtcagcgc tgtacgagat ggcgtgggag
     1021 tcgtcccgcc acatcccctg ttctttgtag tactgctcca ccgtgtcgat cagcttgtcc
     1081 tcgcggccgg tgagtcggag atatctgagg gtctgctcgt cgaccgggaa gtagccgacc
     1141 gtggctccgt actcgggagc catgttggcg atcgtcgccc gattggccag gggcatgtcg
     1201 gccaggccgg gaccgtagaa ctcgacgaac ttccccacga cgccatgctc ccgcagcatc
     1261 tctgtgaccc gtaggaccag gtcggtggcg gtggctccct cggggagctt gccggtcagc
     1321 cggaagccca ccacctcggg gaggagcatg tagatcggct ggcccaccat ggccgcctcg
     1381 gcctcgatgc cgccgacgcc ccagcccacc acgccgaggc cgttgatcat cgtcgtgtgg
     1441 gagtcggtgc cgacgaggct gtccgggtac aggtgctcgc cgtcgtccca gacgaccttg
     1501 gcgaggtact cgagattcac ctggtggacg attccggtgg ccggtgggac gacgcggaag
     1561 ttcgcaaatg cggactgtcc ccatttgagg aactcgtacc gctcttggtt gcgctcgaac
     1621 tccttggtcg agttgatgat cagggcgtcg ggcctcgcaa aagcgtcgac ctgaaccgag
     1681 tggtcgatca ccaggtcggc ggggatcagc gggttgacct tctgggcgga accctcgtcc
     1741 gatgtcatcc ggacgatcgc ggcgcgcatg gcggcgagat cgaccacggc cgggactccg
     1801 gtgaagtcct ggaggatgac ccgccccggt atgaacggga tctctgactc cttgacgttg
     1861 gtggcgtcgt aagccgcgat ctcacggacg tcggcctcgg tgacgctgat ttcgttgagg
     1921 ttcctcagcg ccgattcgag aaggaccttg atcgaatagg ggagtgactc gacgccctcg
     1981 atgacgtcga gtcgggcgat ctcacgcgcc ccgagaggtg tctccagggt ggctcgagcc
     2041 ccgaatggat cgttggacat ttcagccttt cgggtcaatg aaaacctttg ctttcgagcc
     2101 taatgaagcg aaaggtgttg gccatgggcg ccaccgcccg acaaagcacg cgaaattcag
     2161 gaaacgcaag gtttgcattc ctttgcgagg ctctcgacct ccggtagtta gacccgcata
     2221 tgggcctaat tgcctccgaa gggagggtgc tcgagatggt gggtctactt tcgaggtagt
     2281 tagaaccgga tctgggtcta actaccggag gttcgtgaac cgaccgtcac aggcacctgg
     2341 gtgacgcagt cgtcgtcgag cttctgatgg gggatgacga cggggttgca ggggaccgag
     2401 agtgcgccga agaggccggc cacgatccca cgatcgagag aacagatgac atcgggatga
     2461 ctggtcgccg tttccccgaa cgggcaatgc gaggtgagaa gacgctgacc gtcgatgtcg
     2521 gggtccaccg agaagccgag accggtcatg gcactggcca cggcctggac cgcctcgtcg
     2581 tagcccgggt cgtggggcgc cccgatctca gacgccagct cctggccgta cgccctatct
     2641 accttctccg ctatctccga gaggtt
//
LOCUS       DASUTA010000062         2644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1188167, whole genome shotgun sequence.
ACCESSION   DASUTA010000062 DASUTA010000000
VERSION     DASUTA010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188167"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VFZ80_01205"
     CDS             <1..469
                     /locus_tag="VFZ80_01205"
                     /inference="COORDINATES: protein motif:HMM:NF019626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine/phenylalanine carboxypeptidase
                     domain-containing protein"
                     /protein_id="HEX6286078.1"
                     /translation="HPLRLAKLMRRIDAIAMATDGASFLEVFNSLREDGLSDDDAWTT
                     ASRVFRGSLPEGPPFTKDLGYGKGLILTLVYVRMAIRFGKTDRIPLLFCGKVDLLDMA
                     ALHDLYEEGLVERPEFVPPPFDDLPALATTLAMGRFTAQLDIERLSQDFAHLF"
     gene            complement(545..1288)
                     /locus_tag="VFZ80_01210"
     CDS             complement(545..1288)
                     /locus_tag="VFZ80_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEX6286079.1"
                     /translation="MDVDAYLGLIGLQSPIEPDLTSLERLQRAHLTAVPFENLDVYAR
                     RGVRTDDVWSVGKIVERRRGGWCFELNGAFAALLSELGYEVTRHAATVLFGGTTAIPT
                     HLTLRVELDRPYLVDVGFGDSFIKPLPLDSEGPHDGGSGRFGFVFEDDLTTLVAYEDG
                     SPVPQFRFDSKPRQPEDFEEASLLLQTQPGLQWTKTRFATRLIDRGSDRVTLLDGRIK
                     HRRDGVWTEIPVDDEEWDSLLEEWFGLTP"
     gene            1343..2476
                     /locus_tag="VFZ80_01215"
     CDS             1343..2476
                     /locus_tag="VFZ80_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013285081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEX6286080.1"
                     /translation="MIEPFRIDVPESELVDLAERLFRTRLGSAPVGEAWDSGVDYAYL
                     NELIDYWRDGYDWRAKERRLNEYQHFLATIEGQTLHLVRVEADRSRYETATPIILSHG
                     WPYSFNEFLEFARFLSDPLTYDGVADDAFDVVVPSLPGFCFTPPMDDPFTDGAVARLW
                     HRLMTEALGYERFATYGEDVGATISDWLGALYPDSVIGVFATHAAFPPDERAQDPTDA
                     EEDFRAWLAEKWKTARGYAEIQSTRPDTLAVALNDSPAGLLAWMLEKFVEWSGPDFES
                     SWTRDDILTTVSLYWFTQSIGTSFLPYYPGRKNDKPLPLVNVPVGVAVQWGERGFPRE
                     YAERTYTDLRYWTGLPSGGHFTAKQSPDLVARAMREFFSALRD"
     gene            complement(2457..>2644)
                     /locus_tag="VFZ80_01220"
     CDS             complement(2457..>2644)
                     /locus_tag="VFZ80_01220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX6286081.1"
                     /translation="IAAALGGSAAAGVAGLGLSWLRRRTGSLLAPWLVHATVNSAAYL
                     AGVVRSRSGGDLNHAAR"
BASE COUNT          472 a          850 c          837 g          485 t
ORIGIN      
        1 ccacccactt cgtctcgcca agctcatgcg gcgcatcgac gccatcgcca tggcgaccga
       61 cggtgccagt ttcctcgagg tgttcaacag cctccgagag gacggcctct ccgacgacga
      121 tgcctggacg actgcgagcc gggtctttcg gggaagtctc cccgaaggcc ctcctttcac
      181 caaagatctg ggctacggca aggggctcat tctcaccctg gtctatgtga ggatggcgat
      241 caggttcggc aagaccgatc gcataccgct cctcttctgt ggaaaggtcg atctgctcga
      301 catggccgct ctccacgacc tctatgagga ggggctggtg gagcgcccgg agttcgtgcc
      361 tcctcccttc gacgatcttc ccgcgctggc gaccaccctg gccatgggca gattcaccgc
      421 acagttggac atcgagcgtc tctcccagga cttcgcccac ctcttctgag acttgtcccc
      481 acgtttctgg gcgtagaaat caccggtatc cggacatttc tccgcccaga agcagagagc
      541 ggggtcaagg ggtgagcccg aaccactcct cgagcaggct gtcccactcc tcgtcgtcga
      601 ccgggatctc ggtccacacc ccgtcacggc gatgcttgat ccggccgtcg aggagggtga
      661 cccggtcgga tccacggtcg atgagccggg tggcgaatcg agtcttcgtc cattgcaacc
      721 ccggctgggt ctgaaggagc aatgacgcct cctcgaagtc ctccggctga cggggcttcg
      781 agtcgaatcg aaactggggc accgggctcc cgtcctcgta tgccacgaga gtggtgaggt
      841 cgtcctcgaa gacgaagccg aacctcccgc tgccgccatc gtggggcccc tccgagtcga
      901 gggggagcgg tttgatgaag gagtccccga acccgacatc gacgaggtaa ggacggtcga
      961 gctcgacccg gagggtgaga tgggttggga tcgccgtggt cccaccaaag aggacggtcg
     1021 ccgcatgacg ggtcacctcg tagccgagct cgctcagaag ggctgcgaat gccccattca
     1081 gctcgaaaca ccatccccct cgacgtctct cgacgatctt gcccaccgac cagacgtcat
     1141 cggtcctcac cccgcgacgg gcatagacgt cgaggttctc gaatggcacc gcggtgagat
     1201 gggcccgctg caatcgttcc aggctggtca ggtcgggctc gatcggcgac tgcagtccaa
     1261 tgagaccgag atatgcgtcc acatccacgg gaggattgtc tcacgggctc cgacccaccg
     1321 gcgccggggc tagcttcgag gggtgatcga gccgtttcgt atcgatgttc ccgagtccga
     1381 actcgtcgac ctcgcagagc ggcttttccg gacccggctt ggctcggctc ccgtggggga
     1441 ggcgtgggac tcgggtgtcg actatgcgta tctgaacgag ctcatcgact actggcggga
     1501 cggctacgac tggcgggcca aggagagacg tctcaacgag tatcaacact tcctcgccac
     1561 catcgagggg cagaccctcc atttggttcg cgtcgaggcg gatcgcagtc gctacgagac
     1621 cgcaacgccg atcatcctca gccacggatg gccctactcc ttcaacgagt tcctcgagtt
     1681 cgcccggttt ctcagcgatc cgctgacgta tgacggcgtg gcagatgacg ccttcgacgt
     1741 cgtcgtcccc tcgctgcccg gtttctgctt caccccgcca atggacgatc cgttcaccga
     1801 tggtgccgtg gccagactct ggcatcgact gatgaccgag gctctcggct atgagcgttt
     1861 cgccacttat ggcgaggacg tgggtgccac gatcagcgac tggttgggag ccctctaccc
     1921 cgattcggtg atcggcgtgt tcgccacgca cgccgccttt ccgccggatg agagagctca
     1981 ggacccgacc gatgcggagg aggacttccg ggcatggctg gccgagaaat ggaagacggc
     2041 caggggttat gccgagatcc agtcgacgcg acccgacaca ctggcggtgg ctttgaacga
     2101 ctccccggcg ggtttgctgg cgtggatgtt ggagaagttc gtcgagtgga gcgggcccga
     2161 cttcgagagc tcctggactc gcgacgacat cctgacgacg gtgagtctct actggttcac
     2221 ccagagcatc ggcacctcct tcctccccta ctacccgggc aggaagaacg acaagccgct
     2281 tccgttggtg aacgtgccgg tgggagttgc cgtgcaatgg ggggagagag gattccctcg
     2341 tgagtatgcg gagcgcactt acaccgacct ccgatactgg acgggtctcc ccagtggtgg
     2401 gcatttcacc gccaagcagt ctccggatct ggtggcgagg gcgatgcgag agttcttcag
     2461 cgcgctgcgt gattgaggtc gcccccggag cggctccgaa ccacacccgc caggtaggcg
     2521 gctgagttca ccgtggcatg gacgagccac ggggcgagaa ggctgccggt gcgacgacgc
     2581 agccaggaca ggcctaaacc ggcgacaccg gcggcagccg agccaccgag ggccgccgca
     2641 atct
//
LOCUS       DASUTA010000063         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_281542, whole genome shotgun sequence.
ACCESSION   DASUTA010000063 DASUTA010000000
VERSION     DASUTA010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281542"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="VFZ80_01225"
     CDS             <1..1434
                     /locus_tag="VFZ80_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX6286082.1"
                     /translation="TVDAASRSDGGAGSHPVIATLERVDSEQQGEVVTVRARYVVGCD
                     GAHSTVRHAIGLSMQGDSANQAWGVMDVLAVTSFPDIRLKTLIRSANHGSLLIIPREG
                     GYLTRVYIELARLSPGERVADLGITVEGVIDAARMVFRPYPFDVKEVAWWSVYEIGQR
                     LCDRFDDVGHDVSGTRMPRVFVVGDACHTHSPKAGQGMNVAIRDAFNLGWKLAAVLRG
                     QSSPEILHTYSDERHAVAKELIDFDRVFAEMMGAPPKTEAEPDGVDPVEFEHYFARQG
                     RYTAGTTIRYDPSVLTSASSHLDLVEGFPIGMRFHSAPVIRLADAKPVELGHVIKADG
                     RWRLMVFGDRDDPASPGSAMAQLCGWLADGIDSPVRRYGPPNADIDSVIDVRAVFQQN
                     HRTLALEAMPSLLLPRKGRLGLRDYEKMFCADSRDDRDIFDLRGIDRDRGCVVVVRPD
                     QHVSHVLALDAHAELAAFFDAFMIDQS"
     gene            1467..2084
                     /locus_tag="VFZ80_01230"
     CDS             1467..2084
                     /locus_tag="VFZ80_01230"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6286083.1"
                     /translation="MQEVRTAQRGSREMEIIRSAYRTMARRGSHRMSLQDVADDAGVS
                     KALVLYHFGTKDALLLSAMKWAVERTAVRIRQGLETGGSPEEQITALVDAVFIDPEAN
                     RDFYLFYLDLIEHAGRIPSFGELSVMLIDIINGLYRDVIANGVEKGDFHVTDLDVAAR
                     NMRALIEGTFLQWMQTDDWRDNHGEWKNQCRLGILRLLGATGSSR"
     gene            complement(2074..>2383)
                     /locus_tag="VFZ80_01235"
     CDS             complement(2074..>2383)
                     /locus_tag="VFZ80_01235"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX6286084.1"
                     /translation="VIRWTEEAEAELHRRLGIIDQTFGATPRVRTEFEFNDVLRFDDV
                     VDITLSIAAVGNTSVTYRIEVGRDSAKVASGQMVAVFIDRETGEKTPWSPSLREALSG
                     "
BASE COUNT          429 a          748 c          763 g          443 t
ORIGIN      
        1 acagtcgacg ccgcgagccg gagcgacggg ggagcggggt cgcatccggt catcgcaacc
       61 ctcgagcggg tcgattccga gcaacagggc gaggtcgtga ccgtgcgcgc tcgctacgtg
      121 gtgggatgtg acggggccca cagcactgtg cgccatgcca tcggtctgtc gatgcaaggg
      181 gattcagcca accaggcctg gggggtgatg gacgtgctcg ctgtcaccag tttccccgac
      241 atccgtctca agacactcat caggtcggca aaccacggaa gcctcctcat catcccccga
      301 gaaggcggct atctgacccg ggtgtacatc gagctcgccc gtctcagccc gggcgagcgg
      361 gtggccgacc tcggcatcac ggtcgaaggc gtcatcgacg ccgctcggat ggtgttccgg
      421 ccttacccct tcgatgtcaa agaggtggcc tggtggtcgg tctatgagat cggccagcgc
      481 ctctgtgaca ggttcgacga tgtaggacac gacgtcagtg gcacccggat gccccgtgtc
      541 ttcgtggttg gggacgcttg tcacacgcac agccccaagg cgggccaggg gatgaacgtc
      601 gccatccgcg atgcattcaa cctgggttgg aaactggccg cggtgcttcg agggcagtcg
      661 tctccggaga ttctccacac ctactcagac gagcgacacg cagttgccaa agaactgatc
      721 gacttcgatc gtgtcttcgc agagatgatg ggcgcacccc cgaagaccga agcggagccg
      781 gacggggtgg accctgtgga gttcgagcac tacttcgcca ggcaaggtcg atacacggcc
      841 gggacgacca ttcgatatga cccttccgtg ctcacctcgg cgtcgtccca cctggacctg
      901 gttgagggtt tcccgatcgg aatgcgtttc cactcggcgc cggtcatccg gctcgcagat
      961 gccaagcctg tggagttggg ccatgtcatc aaggccgacg ggcgttggcg tctcatggtc
     1021 ttcggagacc gcgacgatcc ggcgtcaccg ggctctgcca tggcgcagct ctgcgggtgg
     1081 ctggccgacg gaatcgactc tcccgtacgg aggtacggac ctccgaacgc agacatcgac
     1141 tccgtgatcg acgtgcgcgc cgtctttcaa cagaaccacc gaacgctggc cctcgaggcc
     1201 atgccgtcgc ttctcctgcc acgcaagggc cgactcgggc ttcgcgacta cgagaagatg
     1261 ttctgcgccg actcgaggga tgatcgggac atcttcgatt tgagaggaat cgaccgggat
     1321 cggggttgtg tggtggtggt ccgacccgat cagcacgtgt cccatgtgct ggcactagac
     1381 gctcacgccg agctggctgc cttcttcgac gccttcatga tcgatcagtc gtaggcgggc
     1441 ggccggccac cggtaccctc ggcccgatgc aggaggttcg gacggcgcag cggggttcac
     1501 gcgagatgga gatcatccgg tccgcctacc ggaccatggc gaggcggggc tcgcatcgaa
     1561 tgtctctcca ggatgtggcc gacgacgcgg gcgtctccaa ggcactggtc ctctatcact
     1621 tcgggaccaa ggacgccctc ttgctttcgg ccatgaaatg ggcggtggaa cggactgcgg
     1681 tgcgaatccg ccagggtctc gagaccggcg ggtcccccga ggagcagatc acagcgctgg
     1741 tcgatgccgt tttcatcgac ccggaggcaa accgggactt ctatctcttc tacctcgacc
     1801 tgatagagca cgccggcagg atcccgagct ttggcgagct ctcggtgatg ctgatcgaca
     1861 tcatcaatgg cctctatcgg gatgtgatcg ccaacggtgt cgagaagggg gactttcatg
     1921 tcaccgatct cgatgtggcc gcccgcaaca tgcgggctct catcgagggg acgttcctcc
     1981 aatggatgca gaccgacgac tggcgggaca accacggaga gtggaagaat cagtgccgtc
     2041 tcggcattct caggctgctc ggagccacgg gatctagccg ctgagcgcct cgcgcagtga
     2101 cgggctccat ggggtcttct cacccgtctc acggtcgatg aacaccgcca ccatctgccc
     2161 cgacgccacc ttcgccgagt ctcgacctac ctcgatccga taggtcaccg aggtgttccc
     2221 cacagccgcg atggataggg tgatgtcgac cacatcgtcg aaacggagca cgtcattgaa
     2281 ctcgaactcg gttcgaaccc tgggcgtggc cccgaaggtc tgatcgatga tcccgaggcg
     2341 gcgatggagc tcggcttcgg cctcttcggt ccaccgtatg acg
//
LOCUS       DASUTA010000064         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_22844, whole genome shotgun sequence.
ACCESSION   DASUTA010000064 DASUTA010000000
VERSION     DASUTA010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22844"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFZ80_01240"
     CDS             <1..336
                     /locus_tag="VFZ80_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6286085.1"
                     /translation="ADFQIPVDVVSVGDVAVDERTRAVVAAASEAMVNAAKHSGADRM
                     SLFVEVEDNVLEVYVTDQGKGFALSSIHADRRGISESIRSRMEKVGGTADIESEPGEG
                     TEVLLRLEL"
     gene            333..965
                     /locus_tag="VFZ80_01245"
     CDS             333..965
                     /locus_tag="VFZ80_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6286086.1"
                     /translation="MTKVYLVDDHRLFLSGVKAELSEEFRIVGTAYDVDTAIEEIRGY
                     HPDVVLVDVHMPDGGGIAVLEAVAETNPDVKFLALSVSDQAEDVIAMIRAGARGYVTK
                     TIEPEELSDAIRRVDAGDAVFSPRLAGFVLDAFAGAIPSDDDPELDQLTSREKDVLRL
                     IARGFAYKQVARKLNISIKTVETHVSSVLRKLQLSNRHELARWASKRRIV"
     gene            1154..>2024
                     /locus_tag="VFZ80_01250"
     CDS             1154..>2024
                     /locus_tag="VFZ80_01250"
                     /inference="COORDINATES: protein motif:HMM:NF015951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEX6286087.1"
                     /translation="MVRSLVGGFLMGAADLVPGVSGGTIALVIGIYERLIASVRQGSL
                     ALGSLIKADFTKFTEHFRAVEWRFLIPLLIGILTAVISLARVLEHQLDVNPEIMAGGF
                     FGLVVGSVLIAWRLIRRPEPMHIAVALVVAVILFVLLGLGEDTNVADPTLLAFFGAGA
                     LAICAMILPGISGSLILLLIGMYAPVLAAVNDRDYLSVGVFVLGATIGLALFSQVLHW
                     ALQQHHDVVLAGLVGLMAGSLRVLWPWPDGVDSPALEAPDGAVVGVIVAGLVGFGFVY
                     LIARLATSRLPVSE"
BASE COUNT          341 a          607 c          635 g          441 t
ORIGIN      
        1 gccgactttc agatacccgt cgatgtggtc tcggtcggcg acgttgccgt cgacgagcgg
       61 acacgagcgg tggttgcagc tgcctcagag gcgatggtca atgccgccaa gcactcgggt
      121 gccgatcgga tgtcgctctt cgtggaggtc gaggacaacg ttctcgaggt atacgtcacc
      181 gatcagggaa aaggattcgc tctcagctcg attcacgctg accgtagggg gatcagcgag
      241 tcgattcgct ctcggatgga gaaggtcgga ggcaccgcgg acatcgagtc cgaacctggc
      301 gaaggaaccg aagtcctgct gaggttggag ctgtgaccaa ggtctatctc gtcgacgatc
      361 accgcctctt tctctccggg gtcaaagcgg agctttcgga ggagtttcgg atcgtcggca
      421 ccgcatacga cgtcgacacc gccatcgaag agatccgtgg ctaccacccc gacgttgttt
      481 tggtcgacgt ccacatgccc gacggtggtg gcatcgccgt actggaagcg gtggccgaaa
      541 ccaacccgga cgtcaagttt ctcgctcttt ccgtctccga tcaggccgag gacgtgattg
      601 cgatgatccg tgctggtgct cgagggtatg tcaccaagac gatcgagcct gaggagctct
      661 cggacgcgat aaggagagtg gatgccggcg acgccgtctt ctcaccgagg ctggctggct
      721 tcgtgctcga cgcctttgcc ggtgcgattc cgagcgacga cgaccccgag ctcgatcagc
      781 tgacttcgcg ggaaaaggac gtcctccgcc tgatcgccag gggattcgcc tacaagcagg
      841 tcgctcgcaa gctgaacatc tccatcaaga ccgtggagac ccacgtctcc tcggtgctgc
      901 gcaaactcca actatccaac cgtcacgagt tggcccgctg ggccagcaaa cgtcggatcg
      961 tctgaacgcg acctccggta gttagaccca catgggggtc taactacctc gagagggtcc
     1021 gggtagcgca tcgctttccg aactctggag gtagatggac ccgtatacgg gtccaactac
     1081 cggaggtcgc ggatgatcaa cgatgcttgg cctacctcat ggccccggcc atactcgccg
     1141 ggttgtgatc gagatggttc gctccttggt cggaggcttt ctcatgggag ccgccgactt
     1201 ggtcccaggt gtgtccggag ggacgatcgc cctcgtcatc gggatctatg agcgtctgat
     1261 cgcctcagtg agacagggga gcctggcact gggatctctg ataaaggccg acttcaccaa
     1321 gttcacagag catttccggg ccgtggagtg gcgattcttg atcccgctgt tgattggcat
     1381 cctgaccgcg gtcatctcat tggcccgcgt gctcgagcat cagctcgatg tcaaccccga
     1441 gatcatggcc ggcgggttct tcggtctcgt ggtcggctct gtgctcattg cctggaggtt
     1501 gattcggaga cccgagccca tgcacatcgc cgttgccctg gtggtggcgg tgatcctgtt
     1561 cgtcttgttg ggattgggcg aggatacgaa tgtcgccgac cccacgttgt tggccttttt
     1621 cggtgccgga gcgctcgcca tctgcgcaat gatcctgccc gggatatccg gctcactcat
     1681 tcttctcctc atcgggatgt atgccccggt gttggctgcg gtcaatgacc gggactacct
     1741 gtcagtcggc gtcttcgtct tgggtgccac gatcggcctc gccctcttct cacaggtgct
     1801 ccactgggct ctacagcaac accatgacgt cgtcctcgcc ggcctggtgg gcctgatggc
     1861 cgggtcgctg cgcgtgctct ggccatggcc ggacggggtc gacagtcccg cattggaagc
     1921 gcccgacggt gcagttgtcg gggtgatcgt tgccgggctc gtaggattcg gattcgtcta
     1981 tctgattgcc cgtctcgcca cttcccggct gcctgtcagc gagc
//
LOCUS       DASUTA010000065         2452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_670529, whole genome shotgun sequence.
ACCESSION   DASUTA010000065 DASUTA010000000
VERSION     DASUTA010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670529"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFZ80_01255"
     CDS             complement(<1..273)
                     /locus_tag="VFZ80_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286088.1"
                     /translation="METSERADARTGRQSALGHLHIPWKTTLLVVPLAVVSLIAGLFG
                     VWATDGNDVAWWILAGSIAVTALGTLLAAYLMGRSMSRGVAELEAAT"
     gene            complement(392..1780)
                     /gene="zwf"
                     /locus_tag="VFZ80_01260"
     CDS             complement(392..1780)
                     /gene="zwf"
                     /locus_tag="VFZ80_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX6286089.1"
                     /translation="MPGPKIPGADALILFGATGDLTRRRLIPALYELAEDGDLDMPVL
                     GVSRRDVGDDGFRRMAATALEEARVDVDESVLSALLANLSYVQGDYRDPSTFERIEEH
                     MGDRICTVSFLAIPPDLFDDVIRGLASAGLNHAGRVVVEKPFGRDTMSARKLNEIIHR
                     HYPEERVYRIDHFLGKEALQNLMVFRFSNTMLEPIWNRHFIRGVQITMAEDFGVEGRG
                     AFYDSVGAMRDVVQNHLLVLMSLLAMEPPVSDRPDALRDERVKVLEATKSLRPEDVVL
                     GQYEGYQEEAGVAEGSTTETYFAARIEIDSWRWAGVPWVIRAGKGLHATLTEAVVEFV
                     RPPRMFFADEDSAPGPNRLVFESKPQNRISLALQAKKPGPRIVSEPVELVMDYGKSRA
                     EAQDAYHRLLGDALRGDQSLFARVDGVMEAWRIVEPILDADLKPDVYPRGAPGPISAD
                     TLVSPDWEWLTH"
     gene            complement(1819..2244)
                     /locus_tag="VFZ80_01265"
     CDS             complement(1819..2244)
                     /locus_tag="VFZ80_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX6286090.1"
                     /translation="MRPMTRERALEFLKQGTRTGNVATVRADGRPHVVPVWFIVEDED
                     VLFTTWHTSVKAQNLARDGRAAMSVDLPEPPYAYLLIDGPVSVIDDPEESRRVATAAG
                     ARYMGSERAEEFGNRNGVSGELVVRIHIENFVGADDMTG"
     gene            complement(2249..>2452)
                     /locus_tag="VFZ80_01270"
     CDS             complement(2249..>2452)
                     /locus_tag="VFZ80_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286091.1"
                     /translation="KVSGVIRPLVLGSAYLVAIAGVVAGSVSGSSIAVVVASLVTVGV
                     MAKVAEYMTGRRVVRARMRVSVR"
BASE COUNT          452 a          817 c          741 g          442 t
ORIGIN      
        1 ggtggcggct tccaactctg caacgccacg agacatcgag cgccccatca ggtatgcggc
       61 caggagggtc cccagggcag tcactgcgat cgacccggcg aggatccacc aggcgacatc
      121 gttgccatcg gtcgcccaca cgccgaacag gcccgcgatg agcgatacca ccgccagtgg
      181 aaccaccagc aacgtcgttt tccaggggat gtgcaagtga cctagagccg actgcctccc
      241 ggtccgagcg tcagcccttt cgctcgtttc cattgcactc gacgttaccg gcgaacccgt
      301 acacttcctg gtaacccatc gcgtgaattc gaggtgttgg agcgtgtgca gtgatcgtgg
      361 tcacggcacc gcatctgcac acgctcgagg gtcaatgggt gagccactcc cagtcgggag
      421 agaccaaagt gtcggcggag atcggtccgg gtgcgcctcg tggatagaca tctggcttca
      481 agtcggcgtc gaggatcggc tcgacgatcc gccaagcctc catgaccccg tccacgcggg
      541 caaacagcga ctgatcgcct cgcaaggcgt caccgagcag ccggtggtag gcgtcttgtg
      601 cctcggctcg actcttccca tagtccatga cgagctcgac aggctcgctg acgattcgcg
      661 gtcccggctt ctttgcctgt aaggcgaggc tgatgcgatt ctgcggcttg ctctcgaaca
      721 ccagacgatt tgggcctggg gcagagtcct cgtctgcgaa gaacatgcgg ggagggcgca
      781 cgaattcaac gactgcctcg gtcagggtgg catggagccc cttcccggcg cggatcaccc
      841 aggggacccc tgcccacctc cacgaatcga tctcgattcg agccgcgaag taggtctcgg
      901 tggtggatcc ttccgcaacg cctgcctcct cttggtaccc ctcgtactgg ccgagcacga
      961 cgtcctcggg tcggagcgac ttggtggctt cgagcacctt caccctctcg tcgcgcagtg
     1021 cgtccggacg atcagacacc ggaggttcca tcgccagcaa cgacatcagc accagcaggt
     1081 gattctgaac cacgtcgcgc atcgccccga ccgagtcgta gaaggcccct cgtccttcca
     1141 cgccgaagtc ctcggccatg gtgatctgca cccctctgat gaagtgccgg ttccagatcg
     1201 gctccagcat ggtgttggag aagcgaaaga ccatgagatt ctgaagtgcc tctttgccga
     1261 gaaagtggtc gatgcggtag acgcgctcct cgggatagtg acgatggatg atctcattga
     1321 gctttcgggc gctcatggtg tctctcccga acggcttctc caccacaacc cgcccggcgt
     1381 ggttgagacc cgccgaggcc aacccgcgga tgacatcgtc gaacaggtcc ggcggtattg
     1441 ccaggaacga caccgtgcag atcctgtcgc ccatgtgctc ctcgatgcgc tcgaaggtcg
     1501 acggatcgcg gtagtcaccc tggacgtagc tgagattggc caggagcgcg gacaggaccg
     1561 actcatcgac gtccaccctg gcttcctcga gagcagtggc cgccatcctg cgaaatccgt
     1621 catcaccgac atctcgtctg ctcacaccga ggaccggcat gtccagatcc ccgtcctcgg
     1681 caagttcgta gagcgccggg atcagccgac gtctggtgag atcgccagtg gcaccgaaga
     1741 ggatcagcgc atctgcaccg gggattttgg ggccaggcat ggtgcccaag gtaataaccc
     1801 cgggctccac ggtgatcgtc agcccgtcat gtcgtcggca ccgacgaagt tctcgatgtg
     1861 gattcgcacc accagctccc ctgacacgcc gttgcgattg ccgaattcct cggcgcgttc
     1921 ggatcccatg taccgggcgc ccgccgcggt agccacacgt cgcgactcct ctggatcgtc
     1981 gatgacactg accgggccat cgatgaggag ataggcgtag ggaggctcgg ggagatcgac
     2041 actcatcgcg gcgcgcccgt cccgcgccag gttctgggct ttgacagagg tgtgccaggt
     2101 tgtgaacagc acgtcctcgt cttcgacgat gaaccacacg ggcaccacat gaggccgacc
     2161 gtcagcgcgc accgtcgcca cgttcccggt gcgggttccc tgcttcagga actcgagggc
     2221 ccgctccctg gtcattggtc tcatctggtc atcgaacgga cacccgcatc cgggcccgga
     2281 cgacccttcg cccggtcatg tactcggcga ccttggccat gacgccgacc gtgaccagcg
     2341 aggcgaccac aacggcgatc gagcttccac tgaccgatcc ggcgaccacc ccggcgatgg
     2401 ccacgaggta ggccgagccc aggacgagcg ggcggatcac accgctcacc tt
//
LOCUS       DASUTA010000066         2245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1059299, whole genome shotgun sequence.
ACCESSION   DASUTA010000066 DASUTA010000000
VERSION     DASUTA010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059299"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VFZ80_01275"
     CDS             <1..365
                     /locus_tag="VFZ80_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286092.1"
                     /translation="ALNVGAVALGVATGGLTATIVGLVLSGLLTVVGIESGPDIGLVV
                     GVLAGLGAGGWLAGRRSVHSHRFHGMVTGLILAFMIVVIARLGGSPADTLTVIWLAFL
                     SVVVSGLAGWLAGRSRQT"
     gene            447..824
                     /gene="rnpB"
                     /locus_tag="VFZ80_01280"
     ncRNA           447..824
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFZ80_01280"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1004..1414
                     /locus_tag="VFZ80_01285"
     CDS             1004..1414
                     /locus_tag="VFZ80_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HEX6286093.1"
                     /translation="MIPRRTDHEHPWGDADTPYIEIGGEEAVRSLAEAFYDVIEEESP
                     ALRNMLPANTANTRKKFFKYLTGWLGGPPLYENEYGHPRLRMRHMPFPIGAEEAEEWM
                     RCMRQALERIGLQDPIRSFLDERFEPLAQHMRNR"
     gene            1480..2163
                     /locus_tag="VFZ80_01290"
     CDS             1480..2163
                     /locus_tag="VFZ80_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX6286094.1"
                     /translation="MCGRFSITGDLDFYAEYFGVDDVLTAPLDKSWNVAPTDQVYVVA
                     EREGSRQLGEMRWGLVPHWAKDNRTIHINARSETVATTPAFRDSFARKRCLIPADGFY
                     EWEPPEHGRTPHWVFRADGHPMVFAGIWASRFDEDAGEWLRTCSIITAESTGAISRIH
                     DRMPVALVPEVWDAWLDQDLRDPEAALGLIQEIEPDSIMEHIVSSRVNSVRNNSPDLR
                     ERVERETLF"
BASE COUNT          413 a          694 c          746 g          392 t
ORIGIN      
        1 ctgccctcaa tgtcggagcc gtcgccctcg gagtcgcaac cggcggcctg acggcgacga
       61 tcgtgggact cgtgttgagc gggctcctca ccgtcgtcgg catcgagtcc ggacccgata
      121 tcggtctggt tgtcggagta cttgcaggct tgggtgccgg cggctggctg gcgggtcgac
      181 gctccgttca ctctcaccga ttccacggga tggtgaccgg gctgatcctg gccttcatga
      241 tcgttgtcat cgcccgtctc ggcggcagcc ccgcagacac gctgacggtg atctggctcg
      301 ccttcctgtc tgtggtcgtc tccgggttgg ctggatggct tgcgggccgg agcaggcaga
      361 cctgaccggc atctcttctg cccgccggta ggacattgcg aaattcgcaa ctggtgccct
      421 gggcaggccg agcggtagaa ttgggaagag cccgtcggac ggtcgcggcg tctcgcatcg
      481 cgaggcgacg aggaaagtcc ggactccaca gggcagggtg ctggataact tccaggcggg
      541 gtgacccgac ggaaagtgcc gcagagaaca gaccgccgat ggcgtcgggt cctcgaccac
      601 gacgaacagg caagggtgaa acggtggtgt aagagaccac cagcgtcgcc ggcaacgggg
      661 acggctggca aaccccaccc ggagcaaggc caagcaggga cgtcggtagc ccgccgaatg
      721 gtcccgggta ggccgcatga gggtcctggt gacagggctc cgagatggat gaccgtcgcc
      781 tgcggaggca ggcacagaat ccggcttaca gacgggctcg aatcgtggag aggctcccaa
      841 aagggggcct ctcctctgtt acagcagagg gccaatcccg aggggtgcgc accggccgaa
      901 atcgagccga ttcgctccgg atacgccgtg gcgtgtcgaa caaattcgag acgccgacac
      961 gtcagcccca cgttcgtgga attggctcat accctgtctc tggatgatcc cgcgcagaac
     1021 cgatcacgag cacccgtggg gtgacgccga cacaccgtat atcgagatcg gtggcgagga
     1081 ggcagtgcgt tcgctggccg aggccttcta tgacgtgatc gaggaggagt caccggccct
     1141 gcggaacatg cttccggcca acaccgccaa tacgaggaag aagttcttca agtacctcac
     1201 gggctggctg ggcggccccc ctctatacga gaacgagtac ggccaccccc gactccgtat
     1261 gcgtcacatg cctttcccga tcggtgccga ggaggcggag gagtggatgc gttgcatgcg
     1321 ccaagcgctg gaacgcatcg gcctgcagga cccgattcgc tcctttctgg acgagcggtt
     1381 cgaaccgctg gcccaacaca tgcggaaccg gtagttggcg gcgcggatga cattgggggc
     1441 cgagatcgac attcgagccc ttctctagtc tcccctcccg tgtgcggccg attctcgatc
     1501 actggcgacc tcgacttcta cgccgagtac ttcggtgtgg acgatgtgtt gaccgccccg
     1561 ctcgacaaga gctggaatgt ggcacccacc gaccaggtct acgtcgtcgc ggagcgcgaa
     1621 ggcagccgcc agctgggaga gatgagatgg gggctggtgc ctcattgggc caaagacaac
     1681 cgcaccatcc acatcaacgc acggtccgag accgttgcga cgacccccgc cttccgagac
     1741 tccttcgccc gtaaacgatg cctcatccct gccgatgggt tctacgagtg ggaaccgcca
     1801 gagcatggcc gaaccccgca ttgggtcttc agggccgacg gtcatccgat ggtctttgcc
     1861 gggatctggg cctcgcgatt cgatgaagat gcgggcgagt ggctgcggac ctgctcgatc
     1921 atcaccgctg agtcgacggg tgcgatctcg aggattcacg accgcatgcc ggtggctttg
     1981 gtccctgagg tgtgggacgc ctggctggac caagaccttc gcgatcccga agcggcgctg
     2041 ggcctcatcc aggagatcga acccgactcc atcatggaac acatcgtctc gagccgggtg
     2101 aactcggtca ggaacaactc acccgactta cgcgaacggg tggagcgcga gacgctcttc
     2161 tgatggcaac ccgccggttg aagcgagagc tggggatggg cggtgcgatc gtcaccggtc
     2221 ttggctcgat agtcggcacc ggtgc
//
LOCUS       DASUTA010000067         3282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1059392, whole genome shotgun sequence.
ACCESSION   DASUTA010000067 DASUTA010000000
VERSION     DASUTA010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059392"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VFZ80_01295"
     CDS             <1..1036
                     /locus_tag="VFZ80_01295"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX6286095.1"
                     /translation="MTPCGTPVTLSEGEPIYEADVVIDPGHGGPVDTGAVAATGLAEK
                     EINLRVSLAVRDLLDERGIAVVLTRIGDYPIPIRTRAEYAQLMGAKALVSIHHNAPAA
                     NPSDIPGVEIFVKHDSAESQRLGGLLYESTMAALGQFDIDWDRAPDAGVMTVLNTEGS
                     DAYGMVRLPEMPSALVELGYIANRAEAELYQRPEYVEVAAAAVADAIEAFLTSDQTGA
                     PLVEGRIFNPQRGVGRDQCVEPDLEAPLYPDVIAVDVSRGSDTYNLEVTISSPYDSPE
                     RYADAFRVVGDDGRVYAIRELLHDHASEQPFTRSVGPVGIPETVRTVTIEARDLVYGW
                     GGETIEVDLP"
     gene            complement(1069..2886)
                     /locus_tag="VFZ80_01300"
     CDS             complement(1069..2886)
                     /locus_tag="VFZ80_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015620129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286096.1"
                     /translation="MTAIAPPASNRTKRGALNTIRDGLSLSPELRRGLIGTIVIAVVA
                     TAGRVVVPIAIQQIIDGGFSEGNVDIPFVARMTGLALVAVLITGGATGWMHLRLARVS
                     ETALSGLRIRAFRHIHDLSMLHQASEQRGVLVTRVTSDVDQISRFMQWAGLQLLVNGL
                     QALIALGVMLVMSVQLGLVVVAMLPIIIFMIHFFQSRLEVAYLTVRERVGQMLALIAE
                     TVVGAPVIRAYGIENRTRERLEEAIENHRLAGTRAGRLSSTFSGFGELTSSLVIAAVV
                     VVGTILAVNGATTEGVVIAFLFLVQLFVEPVQFMGEAVNEAQTAVAGWSRVLDILDIA
                     PDVADPGPDGVTLPHRPLGARFTDVEFRYPRPGESAIEADATPALSDIDVEIPARQRV
                     AIVGETGSGKTTFAKLLTRLMDPTSGTVEVGETDLRKVRFSSLRDRVVMVPQEGSLFR
                     GTIADNVRMGSPEASRDDLLATFERLGLADWLSEMAQGIDTPVGERGGSLSVGERQLV
                     TLVRAAIADPDLLVLDEATSAVDPATEVRINTALNKLTSGRTVVTIAHRLSTAEAADR
                     VLVFDQGRLVQDGPHERLVAEGGVYGRLYESWQRGTTNV"
     gene            complement(2883..>3282)
                     /locus_tag="VFZ80_01305"
     CDS             complement(2883..>3282)
                     /locus_tag="VFZ80_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6286097.1"
                     /translation="FTAARLARADSFIRSLENGYATVVGERGAALSGGQRQRVAIARA
                     LVRKPRLLVLDDATSAVDPAVESEILEGMRQLDTTVVIVAYRRSSIVLADEVIFVEDG
                     RVIDRGTHASLYSSLPAYRDLIDAYEKEPA"
BASE COUNT          613 a         1017 c         1072 g          580 t
ORIGIN      
        1 gatgactccg tgcggcacac cggtgactct cagtgaaggt gagccgatct acgaggcgga
       61 tgtggtcatc gaccccgggc acggtggccc ggtcgacacc ggggccgtgg ctgcgacggg
      121 tctcgccgag aaggagatca accttcgcgt ctcgctggcg gtcagggacc tcctcgacga
      181 gcgcgggatc gcagtggtcc tcactcgcat cggtgactac ccgattccca ttcgcactcg
      241 agccgagtac gcccagctca tgggagcgaa ggccttggtg tcaatccatc acaatgcccc
      301 tgcggcgaac ccctcggaca tccccggtgt cgagatcttc gtcaagcacg actcagcgga
      361 gtcgcaacgt ctgggtgggc tgctctacga gtcgacaatg gctgcactcg ggcagttcga
      421 catcgactgg gatcgagcac cagacgccgg ggtgatgacc gttctcaaca cggagggctc
      481 tgacgcatat gggatggtcc gtttgccgga gatgcccagt gccctcgttg aactggggta
      541 catcgccaac cgcgccgagg cggagctcta tcagagaccg gagtacgtgg aggttgcggc
      601 agccgcggtg gctgacgcaa tcgaggcgtt tctcacaagc gaccagaccg gtgcgccgtt
      661 ggtggagggg aggatcttca acccccaacg cggcgtcggt cgcgaccagt gtgtcgagcc
      721 cgatctcgag gctcccctct atcccgacgt catcgcggta gacgtgagcc gggggtcgga
      781 cacctacaac ctcgaggtga ccatcagctc gccgtacgac tcaccggagc gttatgccga
      841 cgccttccgg gtcgtcggcg acgacgggag ggtctacgca attcgagagc tgttgcacga
      901 ccacgccagc gagcagccgt tcaccaggtc ggttggacct gtggggatcc ccgagacggt
      961 gagaaccgtg accatcgagg cgagagatct ggtctatggc tgggggggcg agaccatcga
     1021 ggtcgatctc ccctaggtgt ggttccgtct cgagcatggg cgaagcattc agacgttggt
     1081 cgtccctcgc tgccaggact cgtagagacg cccatagact ccaccttcgg ccaccagcct
     1141 ctcgtgcggg ccgtcctgga ccagcctgcc ctgatcgaag acgaggaccc tgtcggcagc
     1201 ctcagcagtg gagagacggt gcgcgatggt cactactgtt cgccccgacg tcagcttgtt
     1261 gagggctgtg ttgatgcgca cctcggtcgc gggatcgacc gcggaggtcg cttcgtcgag
     1321 cacgaggaga tcggggtcgg cgatggcggc gcgcaccagc gtcaccaact gccgctcgcc
     1381 aacggataga gagccccctc gctcgcccac aggggtgtcg atgccctgtg ccatctcgga
     1441 gagccaatcg gccagaccca gtcgctcgaa tgtggcaagg aggtcgtcac gactggcctc
     1501 cggggagccc atgcggacat tgtccgcgat cgtccccctg aagagactgc cttcctgggg
     1561 aaccatcacg acccggtcgc gcaaagagga gaacctgacc tttcgcaggt cggtctcgcc
     1621 gacctccacg gtcccactgg ttggatccat cagcctggtc agcaatttgg cgaatgtggt
     1681 tttccccgag ccggtctcac cgacgatggc cacccgttga cgcgccggga tctcgacgtc
     1741 gatgtcgctg agtgcagggg tggcgtcggc ttcgatggcc gattcacccg gccgcgggta
     1801 gcggaattcg acatcggtga accgagctcc caagggacga tggggcagtg tcaccccatc
     1861 aggaccggga tcggcgacgt ctggcgcgat gtcgaggatg tcgaggaccc ggctccaccc
     1921 ggcgacggct gtctgtgctt cgttgacggc ctcgcccatg aattggaccg gttcgacgaa
     1981 tagctggacc aggaacaaga aggcgatcac cacgccttcg gtggtggcgc cgttgaccgc
     2041 gaggatggtg ccgaccacca ccacggcagc gattacgagg gacgaagtca gctctccgaa
     2101 accggagaag gtcgatgaga ggcggccagc tcgtgtgcct gccagtctgt gattctcgat
     2161 tgcctcttcg agacgctccc tggtgcgatt ttcgatcccg taagcacgga tgacaggagc
     2221 gccaacaacc gtctccgcaa tcagcgccag catctgaccg acccgctccc gaaccgtgag
     2281 gtaggcgact tcgagccggc tctggaagaa gtggatcatg aagatgatga tcgggagcat
     2341 cgccaccacg acgagaccga gctgtacgga catcacgagc atgaccccga gggcgatcag
     2401 ggcttggaga ccgttgacca ggagctgcag accggcccat tgcatgaaac gactgatctg
     2461 gtcgacatcg gaggtgactc tggtcacgag cacgccacgc tgctccgagg cctgatggag
     2521 catggagaga tcgtggatat ggcgaaaggc gcggattcga agcccggaaa gagccgtctc
     2581 ggagactctc gccagccgga ggtgcatcca gccggtggct ccgcccgtga tcaagacggc
     2641 gaccaaagcg agtcccgtca tgcgggccac gaacgggatg tcgacgtttc cttcgctgaa
     2701 acctccgtcg atgatctgct gtatcgcaat tgggaccacc acccgtcccg cagtagccac
     2761 cactgcgatg acgatggtgc cgatcagccc tctgcgaagc tcgggggaga gggagaggcc
     2821 atctctgatc gtgttgagcg ccccgcgctt ggtccggttc gacgcaggag gagctattgc
     2881 ggtcacgccg gctccttctc gtaagcgtcg atcaagtctc gatatgcggg aagggacgag
     2941 tagagggagg cgtgggtgcc cctatcgatg acccgtccgt cctccacgaa tatcacctcg
     3001 tcggcgagga cgatcgacga ccggcgataa gcgacgatga ccaccgtagt gtcgagctgt
     3061 ctcatacctt cgaggatctc gctctcgact gcggggtcga ccgctgaggt ggcgtcgtcg
     3121 aggacgagca gccggggctt gcgcaccagg gccctcgcta tagccactcg ttggcgttgc
     3181 cctcctgaca gtgccgctcc ccgttcgcca acgaccgtgg catagccgtt ctcgagtgat
     3241 ctgatgaatg agtcggcgcg agccagtctg gctgccgtga ac
//
LOCUS       DASUTA010000068         2662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_800203, whole genome shotgun sequence.
ACCESSION   DASUTA010000068 DASUTA010000000
VERSION     DASUTA010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800203"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VFZ80_01310"
     CDS             complement(<1..365)
                     /locus_tag="VFZ80_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6286098.1"
                     /translation="MSHPSELISAYLDGELHGSELKSLLDHLAGCGRCAADLQDIQRV
                     RSAIRSLPVLELPEGIVPEADAEIVPIHRNRGLWVGAAAAVVAVVIAVAALVTPAPEP
                     VSLEDLTSRFGARASLDPS"
     gene            complement(362..862)
                     /locus_tag="VFZ80_01315"
     CDS             complement(362..862)
                     /locus_tag="VFZ80_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6286099.1"
                     /translation="MESIPLTWEEVARVHGRKIYNFAYRLTGNADDAHDLVQEVLLRV
                     RRGLDNYQPGSFEGWLWRITRNAFLDDVRRKKRRPTSPMPDEVDRWDLASAESADIEY
                     DRVSLGDDIQKALLELPIDFREAVVLCDVVGLSYEEIAVAVAVPIGTVRSRIHRGRKM
                     LKDLLS"
     gene            complement(919..1143)
                     /locus_tag="VFZ80_01320"
     CDS             complement(919..1143)
                     /locus_tag="VFZ80_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX6286100.1"
                     /translation="MNLRGQELLIILLIVVLIFGARKLPELARSLGSSAKEFRKGIEE
                     GSKEDPEDAPVTDATESDPAASDIPDAKDS"
     gene            complement(1209..2162)
                     /locus_tag="VFZ80_01325"
     CDS             complement(1209..2162)
                     /locus_tag="VFZ80_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286101.1"
                     /translation="MEPFIFVVVLVAIGLLLFNMFRNRKRRMQEAEKESKTAAARGLS
                     VSRDLFEAEVGHHAPLESFHVVGDEARVTFDVPLDDEDDEVLNDLLDGEAIEVVRQKR
                     HTLPIDDVKEIVVYAGREKRREVARHELPAPGELPPPLLESGMSFSNIAHDPFAAHFD
                     EGADLGLTPGTAVQVPEDELPPLVDELRIPKGLDRGLRALGTDPSALGGPEFVIALLR
                     MFGYGVTEQAYDGSYMATKSGVSTYIETDTYRSGDYPELDEVVIRRFLANFGSSGADR
                     GMLISDKYSPFLIHEIETNQPRVRFITRERVQRFIDSMALG"
     gene            complement(2197..>2662)
                     /locus_tag="VFZ80_01330"
     CDS             complement(2197..>2662)
                     /locus_tag="VFZ80_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEX6286102.1"
                     /translation="ALGVGRLPVVASDDPRRLVGMFRREEAVRAYHEALTSTTDHHLH
                     RQRLAQRTQPGAGYYDFRVPIGSIADGRKVRDVSWPEESTLVSVRRGREVLVPDGDTN
                     LIAGDVITAFGTESSRQRMIDRLNAGADEPTAEIPLVVTEEPAEEGTVVGPE"
BASE COUNT          481 a          844 c          845 g          492 t
ORIGIN      
        1 aacgacggat cgagtgaagc acgtgccccg aaccgacttg tgagatcctc caaggagacg
       61 ggttccggag cgggtgtcac cagggcggcg acggcgatga caactgccac cactgccgca
      121 gcggcgccaa cccacagccc cctgttccgg tggatcggga cgatctcggc atcggcttcg
      181 gggacgatcc cctcaggaag ctcgaggacg ggaagggagc gtatggccga acgcactcgc
      241 tgtatgtctt ggagatctgc ggcgcatcga ccacagccgg cgagatgatc gaggagagac
      301 ttcagctccg atccatggag ctcaccatcc agataggcgc tgatcagctc ggagggatgg
      361 ctcacgacag caagtccttc agcatcttcc ggcctcgatg gattcggctg cgaaccgtgc
      421 cgatggggac ggccaccgcc acagcgatct cctcgtagga gagcccgacg acatcgcaga
      481 ggacgactgc ctcgcgaaag tcgattggga gctcgagcag agccttttgg atatcgtcgc
      541 cgagggagac cctgtcgtac tcgatgtcgg ccgattccgc cgatgcaagg tcccagcgat
      601 cgacctcgtc gggcatgggc gatgtgggtc gacgcttctt gcgccgcaca tcatcgagga
      661 aggcgttgcg agtgatgcgc cagagccagc cctcgaagct cccgggctga tagttgtcga
      721 gcccgcggcg aacccgaaga agcacttctt gaacgaggtc gtgcgcgtcg tcagcgttgc
      781 cggtgaggcg gtaggcgaag ttgtagatct tccgtccatg gacacgggcg acctcttccc
      841 atgtgagcgg tatggactcc atcttcctgg agacctacat ggacaacgac gccgcgtgga
      901 ggcgggttcc cggcagagct aggaatcttt ggcgtctgga atgtctgacg ccgccgggtc
      961 ggactcggtg gcgtcggtca ccggtgcgtc ctcggggtct tccttcgatc cctcttcgat
     1021 tcccttgcga aactcctttg cggagctacc gagagacctg gccagctcag gcagctttcg
     1081 cgcgccgaag atcaacacga cgatcagcag gattatcagc agttcttgac ctctgaggtt
     1141 caaatagacc tccttccaag ctcaggctat caggtatgcg ctctcaggga taaggcagtt
     1201 gcccgggttc aacccaaggc catcgagtcg atgaagcgct gcactcgctc tcgcgtgatg
     1261 aacctgaccc ggggctggtt ggtctcgatc tcatgaatga ggaaagggct gtacttgtcg
     1321 ctgatgagca tcccacggtc ggcaccggag gagccgaagt tcgccaggaa cctcctgatc
     1381 acaacttcgt ccagctccgg gtagtccccg gacctgtagg tgtcggtctc gatgtacgtg
     1441 gagaccccgc tcttggtggc catgtagctg ccgtcgtagg cctgctcggt gacgccatat
     1501 ccgaacatgc gaagcagggc gatgacgaac tccgggccgc cgagggcgga agggtcggtg
     1561 ccaagtgctc gcagcccgcg atcgagaccc ttgggaatcc gcagctcatc caccagaggg
     1621 ggcagctcgt cctcgggaac ctgaaccgct gttcccgggg tgagcccgag gtcggcgccc
     1681 tcgtcgaagt gggcggcgaa cgggtcgtgt gcgatgttgc tgaagctcat gcccgactcg
     1741 agcagcggag ggggcagctc gccgggagcg gggagctcgt gccgggcgac ctcgcgcctc
     1801 ttctcccggc cggcgtagac cacgatctcc ttcacgtcat cgatgggcag cgtgtgacgc
     1861 ttctgcctca cgacctcgat cgcctcgccg tcgagcaggt cgttgagcac ctcgtcgtcc
     1921 tcgtcgtcga gaggcacgtc gaaggtcacc cgggcctcat cgccgacgac atggaacgac
     1981 tccaaggggg cgtggtgacc cacctcggcc tcgaacaggt cgcggctcac cgacagtccc
     2041 ctggcggcgg ccgtcttcga ctccttctcc gcctcctgca ttcggcgctt gcggttgcgg
     2101 aacatgttga agaggagaag gccgatcgcc accaggacga ccacgaagat gaatggttcc
     2161 acttatccgt ctttcagcta gctggcgtca tcgagtctat tcaggaccga caacggtccc
     2221 ttcttcggca ggctcctctg tcaccacgag aggtatctcc gccgttggct cgtcggcccc
     2281 ggcgttcaag cgatcgatca tccgctgtcg ggagctctcg gttccgaagg cggtgatcac
     2341 gtcaccggcg atcaggttgg tgtccccatc gggaacgagg acctctctcc cccgtcgtac
     2401 cgacaccagg gtcgactcct ccggccatga cacgtcacga accttgcgcc cgtcggctat
     2461 cgagccaatg ggcacccgaa agtcgtagta gccggctcca ggctgggtcc gctgggcgag
     2521 ccgctgccga tggagatggt ggtcggtggt gctcgtcagc gcctcgtgat atgcgcgaac
     2581 ggcctcctca cgtcggaaca tccccaccag ccgcctcggg tcgtcggagg ccaccacggg
     2641 caacctgccc acacctagag ca
//
LOCUS       DASUTA010000069         3419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_152939, whole genome shotgun sequence.
ACCESSION   DASUTA010000069 DASUTA010000000
VERSION     DASUTA010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152939"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1691
                     /locus_tag="VFZ80_01335"
     CDS             33..1691
                     /locus_tag="VFZ80_01335"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX6286103.1"
                     /translation="MPLKVAGAQVNLTVGDLDGNEETIAAAMDRAAATGADVLLLPEL
                     AIPGYPPEDLVLRRDFVTANIETLRRLARRSGSMTTVVGFLDFASGLGANATDAESRE
                     VANAAAVLADGHIKGVYHKIHLPNYGVFDEDRYFVVGDDPDRIWEIAGVPVGVSVCED
                     IWLDEGPPLQQARNGAEVLFNINASPFHRGKAADREAMLAARARAGDAAVVYLNLIGG
                     QDELVFDGASLVVSSDGTILHRSPQFEEDFFVLDVPLRGEGVESGEITPLLEPIEEVY
                     RALTTGLSDYCRKNGFDSVIIGLSGGIDSAVTAAIATDALGSDSVWGVSMPSRFSSQH
                     SIDDARSLAENLGIRFDLIPIDGVYEAHLDSLATLFEGTKFGVAEENLQARSRGATLM
                     ALSNKFGPMVLATGNKSEMAAGYATLYGDMVGGFSVLKDVFKGIVYELARWLNKGDEV
                     IPQNTIDKPPSAELRPNQKDSDSLPPYEILDAILERYIEADMGVEEIVAEGYAPDVVT
                     SVCRMVDRNEYKRRQAAPGVKITTKAFGKDRRLPITNHYRPRLQ"
     gene            1688..2494
                     /locus_tag="VFZ80_01340"
     CDS             1688..2494
                     /locus_tag="VFZ80_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX6286104.1"
                     /translation="MITEVLINGAPSDGLIPVTDSAVLRGDGCFEVLKSYRGKAFALD
                     AHIGRLHASAAALQIELPDRSDLMAWIEKTAAEVGTGAVRVVVTRGASVPGLDDPSNV
                     IVFGHGWDIPDGPARLYPVVAPWHAAGAEWDLAGAKITSYGPNMAAARRARNEGFDDA
                     LLLTTSSVILEGPTFSIAWVVEGVLETPNLDLGILDSITRRLVLDLAREAGTDVVEGR
                     WDLERLESASEAMALSTIREVQPVVAVGETTWDPGPVTDLLARGYAALIG"
     gene            2535..3059
                     /locus_tag="VFZ80_01345"
     CDS             2535..3059
                     /locus_tag="VFZ80_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286105.1"
                     /translation="MTMKIRPIAVFAVLALAVIACDGDDPELTTDSTIVTGGTNAPAT
                     TTTTEAPDDGNGGSSPSTTLVGQTVTDFEVVARFPNDNGEERYYVIPEGAYTDVDLTQ
                     FVIELLENDSELYGAEIFDSVEAAEAYQVEEGERTEEQVTLIESDHFVTLIGRDRIEF
                     HGPFSEFPGGAIGS"
     gene            complement(3090..>3419)
                     /locus_tag="VFZ80_01350"
     CDS             complement(3090..>3419)
                     /locus_tag="VFZ80_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX6286106.1"
                     /translation="SAQVPSILGRQLGVGVLNGAVLGILAGTACWLLINSGLFETNSD
                     VFDVSLVVAVSAFSVLLIGAVVGGGIPVLLRRFGLDPALASSIFLTLITDTVGFGGFL
                     LLAGWFL"
BASE COUNT          657 a         1068 c         1105 g          589 t
ORIGIN      
        1 gccatgttga ggaggaggcg tatcgtgtgg ggatgcctct caaggtggcc ggggcacaag
       61 tgaatctgac tgtgggagac ctcgacggca acgaggaaac catcgcagcc gccatggatc
      121 gcgctgcggc aaccggtgcc gatgtgttgt tactccccga gctggcgatt cccgggtacc
      181 cgcccgaaga tctcgtgctc cgtcgcgatt tcgtgaccgc caacatcgag accctccgcc
      241 gtctcgctcg caggtcgggc tcgatgacca cggtggtggg gttcctcgac ttcgcctccg
      301 ggctcggggc caacgccacc gacgccgagt cgagagaggt ggccaatgcc gcggcagtgc
      361 tcgccgacgg ccacatcaag ggcgtgtacc acaagatcca tctccccaac tacggagttt
      421 tcgacgaaga ccgctacttc gtcgtcggag atgacccgga tcggatatgg gagatcgctg
      481 gtgtgccggt tggtgtgtct gtttgcgagg acatctggct cgatgagggc ccaccgttgc
      541 agcaggcgag aaacggagcg gaggtgttgt tcaacatcaa cgcttcgccg ttccaccggg
      601 gaaaggcggc cgacagggag gcgatgctgg cggccagggc ccgagccgga gacgctgccg
      661 tcgtctatct caacctgatc ggcggccagg acgagctcgt tttcgacggt gcctcactgg
      721 tggtttcctc cgacggaacg attctccacc gctcaccaca gttcgaggag gacttcttcg
      781 tgctcgacgt gcctctcagg ggggagggag tggagtctgg cgagatcaca cctctgctcg
      841 agcccatcga agaggtctac cgggcgttga cgacaggcct ctccgactac tgccggaaga
      901 acggcttcga ctccgtcatc atcggcctct caggcggcat cgactctgcc gtaaccgcgg
      961 ctatcgccac cgatgcgctc gggtcggact cggtgtgggg agtgtctatg ccttcccgct
     1021 tctcatctca gcactcgata gacgacgcac gctctctggc cgagaacctc ggcatacgtt
     1081 tcgacctgat tcccatcgac ggggtctacg aggctcatct cgactccctg gcaactcttt
     1141 tcgaggggac gaagttcgga gtggccgagg agaatctcca ggcacgatcg agaggggcca
     1201 cactgatggc gctctccaac aagttcggtc ccatggtctt ggcgacgggt aacaagagcg
     1261 agatggcggc cggatacgcc accctctacg gagacatggt cgggggcttc tcggtgttga
     1321 aggacgtatt caaagggatc gtctacgagc tggcgagatg gctcaacaag ggagacgagg
     1381 tgataccaca aaacaccatc gacaagcctc cctcagccga gctccgaccg aaccagaaag
     1441 actccgactc gctgcccccc tatgagatcc tcgacgcgat cctggagagg tatatcgagg
     1501 cagacatggg tgtcgaagag atcgtcgccg agggatatgc cccggatgtg gtcacatcgg
     1561 tctgccgcat ggtcgatcgc aacgagtaca agcggcgcca ggctgcgccc ggtgtcaaga
     1621 tcaccacgaa ggcgttcggc aaggacagac gtctcccgat caccaatcac taccggcccc
     1681 gtctgcagtg atcaccgagg tcctcatcaa tggggcccct tctgacgggc tcatccccgt
     1741 caccgactcc gctgtgctgc gaggtgatgg gtgcttcgag gtcttgaagt cgtatcgggg
     1801 caaggccttc gctctcgatg ctcacatcgg acgtctccat gcgagtgcgg ccgctctcca
     1861 gatcgagctg cccgatcgat ctgatctcat ggcctggata gaaaagacgg ccgccgaggt
     1921 gggcaccggg gcagtccggg tggtggtgac tcgaggtgcg agcgtccccg gtctcgacga
     1981 cccctccaac gtgatcgtct tcgggcacgg ctgggacatc ccggacggcc cggcgaggct
     2041 gtacccggtg gtcgctccgt ggcacgcagc cggcgccgaa tgggacctgg ccggcgccaa
     2101 gatcacctcc tatgggccga acatggcggc ggcccgtcgg gcgcggaacg agggcttcga
     2161 tgacgccctg ctgctgacca ccagcagcgt gatactggaa ggcccgacgt tttcgattgc
     2221 gtgggtggtc gaaggggtgc tggagacccc gaacctggac ctcggcattc tcgactcgat
     2281 aacgaggagg ttggtgctgg acctggcccg ggaggccggg accgatgtgg tcgaggggcg
     2341 gtgggatctc gagcgtctgg agtctgccag tgaggcgatg gcgctatcga ccattcgtga
     2401 ggtccaaccc gtcgtcgccg tcggagagac cacgtgggac cccgggcccg tcacggatct
     2461 cctcgccagg ggttatgccg cgctgatcgg ttagttcacc ggttgggaac cgaccggcac
     2521 tagcgtttcg ttcagtgacc atgaagatcc gaccgatagc cgtgttcgcc gtcctcgcgc
     2581 tcgccgtcat cgcctgcgat ggggacgatc cggagctgac cactgactcg accatcgtca
     2641 ccggagggac gaacgcaccc gccaccacga ctaccacgga ggcacccgac gacggcaacg
     2701 gaggatcttc gccatccacg accctggtgg gtcagaccgt caccgacttc gaggtggtcg
     2761 ctcgcttccc gaacgacaat ggggaagagc gctattacgt catccccgaa ggcgcctaca
     2821 ccgatgtcga cctgacccag ttcgtcatcg agctcctgga gaacgacagc gagctctacg
     2881 gagcggagat cttcgacagc gtcgaggcag ccgaggccta ccaggtcgaa gagggggagc
     2941 gaaccgaaga gcaggtcacc ctgatcgaga gtgatcactt cgtcacgctc atcggtcggg
     3001 accgcatcga attccacggc ccgttctccg agttccccgg tggcgccatc ggaagttgat
     3061 gtccagggct cgggtcgatg acccccgagt cagaggaacc agccggcgag cagcagaaag
     3121 cctccgaagc cgacggtgtc ggtgatcagc gtgagaaaga tcgatgaggc cagcgccgga
     3181 tcgaggccga accgacggag caggaccggg atcccaccgc cgaccaccgc gccgatcaga
     3241 agcacgctga acgccgagac cgcgactacc agcgacacat cgaagacgtc cgagttggtc
     3301 tcgaagaggc ccgagttgat cagcagccag caagccgtcc cggcgagaat gccgagcacc
     3361 gcgccgttga gcacgccaac tcccagctga cggccgagga tgctcggtac ctgggccga
//
LOCUS       DASUTA010000070         3160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_671444, whole genome shotgun sequence.
ACCESSION   DASUTA010000070 DASUTA010000000
VERSION     DASUTA010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671444"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFZ80_01355"
     CDS             complement(<1..579)
                     /locus_tag="VFZ80_01355"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HEX6286107.1"
                     /translation="MNVVRNLERRLERLLEGVAGRVFSGRIHPSELAGKLAREADFAR
                     FEHFTGPATANLFVIQVNPDDLSVETSKLEKMLAAEMTAYTAEEGLRLEGPVTVRIEP
                     SARATSGHVNCHVEVVPGPPVVWATLSANGESLDIGRNRATIGRAPDADVVVPHDDIS
                     RRHALIYREHGQTWLRDLSSANGTYVDGVRIDS"
     gene            716..799
                     /locus_tag="VFZ80_01360"
     tRNA            716..799
                     /locus_tag="VFZ80_01360"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:750..752,aa:Leu,seq:cag)
     gene            841..1803
                     /locus_tag="VFZ80_01365"
     CDS             841..1803
                     /locus_tag="VFZ80_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286108.1"
                     /translation="MGRLLLPLLITIALIVAVVVTAAGEETRTEIEYLDEIRGQATEL
                     ARSGSAIRQMMPRLREIGRDEFTTVMDSVTNDVAVSLAFVGSEPPVDSLIPIWSLYRQ
                     AVQAWESGVSTLSTSILQAADQPDDATVANLIGDGLADLRAGDELFDLLRSEVEREEI
                     PDPISPITDVNLFPADGGGLASLAGTYVAAARASTNNLGLLPGLRVSQVLAEPDWDVS
                     VEDEVVIPFTETVVFSVVVTNIGNVESEPGTLSLELVGGEVPVRATAQVRALNPNGQT
                     TIVFEPIEVFPDIAYQVTGELIVDGPDSDPSDNRQSTTFTVNAA"
     gene            1911..3158
                     /gene="serS"
                     /locus_tag="VFZ80_01370"
     CDS             1911..3158
                     /gene="serS"
                     /locus_tag="VFZ80_01370"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006604332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX6286109.1"
                     /translation="MLDPRILRDDPDAVAESLRRRGVDIDLDALVGLEAEYRSVLQEA
                     EELRSRQKDAGKEIANLEGDAKQKAIDEVSGLAASVKEATSRADELADKFRIAWLDVP
                     NLVDPTAADGYTDEDNVAIKHHGDAGTEGTDHATLGEALDIIDTERAAKVSGSRFGYL
                     KGKGALLELSLVRWAMDHLSAAGFTPMIPPVLVREHALEGTGFFPEAREQVYEIPKDE
                     LFLVGTSEVPLAAYHGDEILDSEDLPLRYAGFSTCFRREAGTYGKDTAGIFRVHQFDK
                     VEMFVFTTPDASKDEHERLLEVEEQLVQQLEVPYRVVNVAAGDLGASAAKKYDIEAWF
                     PGEQAFREITSCSNTTDYQARRLKVRYRGESGNTLAHTLNGTACAVGRTILAILENHQ
                     QPDGSVMIPEAIRSYTGFDIIEW"
BASE COUNT          597 a         1021 c          989 g          553 t
ORIGIN      
        1 ggagtcgatc cggacaccat cgacataggt gccattggcg gaggagaggt ctctgagcca
       61 tgtctggcca tgctcgcggt agatcagggc gtgtctccta ctgatgtcgt catgagggac
      121 caccacatcg gcatccgggg cccggcctat cgttgccctg tttctcccga tgtcgagaga
      181 ctcgccattc gcggagagag ttgcccagac cactggcgga cctggcacca cctcgacatg
      241 gcaattcacg tgcccggacg tcgctcgagc gctcggctcg atcctcaccg tgaccggccc
      301 ctcgaggcga agaccctctt cagcggtgta ggcggtcatc tccgcagcca gcatcttctc
      361 cagcttcgag gtctccacgg acaggtcgtc ggggttcacc tggatgacga acaggttggc
      421 cgtagccggc ccggtgaagt gctcgaatcg cgcgaagtct gcctcacggg ccagctttcc
      481 cgccagctcg gaggggtgga tgcggccgga gaagacccgt ccggccacac cttcgagaag
      541 gcgttcgaga cgtcgctcca ggttcctgac aacgttcacg tctggggcag gttagggctg
      601 cgccggggcc atccgactac cggaaggggg tggtgggcgg tacgattctc gttcccgcgg
      661 gatagcatcc cgtggtgtct gacccctgag caacctgatc tcatcagatg cccgcgggcg
      721 agtggcggaa ttggcagacg cgcaggattc aggttcctgt gatcgcaaga tcgtgggggt
      781 tcaagtcccc cctcgcccac cgtggtcttc gatccaccag ccaagtcgag aaggcgggtg
      841 gtgggacgcc tccttctgcc gcttctcatc accattgccc tcatagtcgc cgttgtcgtc
      901 acagcggcgg gcgaggagac gcgcaccgag atcgagtatc tcgacgagat ccggggtcag
      961 gccaccgaac tggcccggag tggttctgcc atcaggcaga tgatgccccg gctgcgcgag
     1021 atcgggcggg atgagttcac cacggtcatg gactcggtca ccaacgacgt tgccgtctca
     1081 ctggccttcg tcgggagtga gccaccggtg gattctctga tcccgatctg gtcgctctac
     1141 cggcaggcgg tccaggcctg ggaatcggga gtgtccacct tgtcgacttc gatcctccaa
     1201 gcggcggacc aacccgatga cgccacggtg gcgaacctga taggagacgg tctcgccgat
     1261 ctccgggccg gagatgagct gttcgacctg ctccgctccg aagtcgagcg ggaagagatc
     1321 ccggacccga tcagcccgat caccgatgtc aacctctttc ccgccgatgg cggagggctg
     1381 gccagtctcg ctgggaccta tgtggccgcg gcccgggcat ccaccaacaa cctcggcctt
     1441 ctgcccggtc tgcgagtgag ccaggtcctg gctgagcccg actgggacgt cagcgtcgag
     1501 gacgaagtgg tgattccatt caccgagacc gtcgtcttct ctgtcgtcgt gaccaacatc
     1561 ggcaacgtcg agagcgagcc gggcaccctg agtctcgagt tggtcggggg tgaggttccg
     1621 gtgcgggcaa ccgcacaggt cagagcgctg aaccccaacg ggcaaacaac gatcgtgttc
     1681 gagccgatcg aggtcttccc cgacatcgcc taccaggtga cgggcgagct gatcgtcgac
     1741 ggccccgact ccgacccctc cgacaaccga cagtcgacca ccttcaccgt caacgcggcc
     1801 taacccctat tccgcgaacc cggggttcct caggccatat acggcccaaa tccgctcatg
     1861 ttcgcccgaa tggggccaat cgggacgctg tcggtagcat cgggcgaccc atgctcgacc
     1921 ccagaatcct cagagacgac cccgatgcgg tggcggaatc gcttcgtcgc cgcggtgtcg
     1981 acatcgacct cgacgccctc gtgggtctcg aggccgagta tcgctctgtc ctccaggagg
     2041 ccgaggagct ccgctcccgt caaaaggacg cgggcaagga aatcgccaac ctcgaaggcg
     2101 acgcgaaaca gaaagcgatc gacgaggtct ccggcttggc tgcgtcggtg aaagaggcaa
     2161 cgtctcgcgc agatgagctg gccgacaagt tcaggatcgc ctggctcgat gtccccaacc
     2221 tggtcgaccc aacggcagcc gacggataca ccgacgagga caatgtcgcg atcaagcatc
     2281 acggcgatgc cggaacggag gggaccgatc atgccaccct cggcgaggcc ctcgacataa
     2341 tcgacaccga gcgagcggca aaggtgtccg gaagccgctt cggctatctc aaaggcaaag
     2401 gagccttact cgagctgtcg ctcgtccgct gggcgatgga tcacctgagt gccgccggct
     2461 tcaccccgat gatcccaccg gttctggtac gcgagcatgc cctcgaaggc accgggttct
     2521 tccccgaggc gagagaacag gtctacgaga ttcccaagga cgagctcttc ctggtcggta
     2581 ccagcgaggt ccccctggcc gcctatcacg gagacgagat cctcgactcc gaagacctgc
     2641 cgttgcgata cgcaggtttc tcgacgtgct tccggaggga ggccggcacc tatgggaagg
     2701 acaccgcggg catcttccgc gtccaccagt tcgacaaagt cgagatgttc gtgttcacga
     2761 cgccggacgc atccaaggac gagcacgagc gactcctcga ggtcgaggaa cagttggttc
     2821 agcagctgga ggtgccctac cgggtggtca acgtggccgc cggtgatctg ggagcgagtg
     2881 ctgcgaagaa gtacgacatc gaggcttggt tcccgggtga gcaagcgttt cgcgagatca
     2941 cctcgtgctc caacaccacc gactatcaag cccgtcgtct caaggtgcgt tatcggggcg
     3001 agtccggcaa cacgctcgcc cacactctca acgggaccgc ttgtgcggtg ggccgcacca
     3061 tcctcgccat cctggagaac catcaacagc cggacggcag tgtgatgatc cccgaggcaa
     3121 tccgcagcta caccggattc gacatcatcg aatggtgagc
//
LOCUS       DASUTA010000071         2826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_930494, whole genome shotgun sequence.
ACCESSION   DASUTA010000071 DASUTA010000000
VERSION     DASUTA010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930494"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1215)
                     /gene="mltG"
                     /locus_tag="VFZ80_01375"
     CDS             complement(115..1215)
                     /gene="mltG"
                     /locus_tag="VFZ80_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX6286110.1"
                     /translation="MTFEPVPDQSRWSRITKVLFIGVGIVLGAVIVVAGASYLGRTVG
                     DALGPQDVTGAPVDVEPGVEVTVEIDPGSTAQDIGAILAAQGVVRSALEFEVAVRNVE
                     AAQRLQAGTYELVTLMDPADVVATLVTGPGASVYRVTVIEGLRVSEILTSLAEDTPHE
                     YSDFETALLDGSVTTSLVEMPENPELQDWEGLLFPDTYEFSAATEPDAILQRLASTME
                     QRVDSIDWSAWEDLGYTPYQGIVLASLVESEVQLDEERPIVSSVIYNRLAEGMALDID
                     ATVLYALGTRDVAEFNRDFDSPYNTYLYPGLPPTPISAPGRASLEAAAAPDDTPFFYY
                     VLSDAEGHHAFAETLEEHNANVEQAREDGILP"
     gene            complement(1212..1661)
                     /gene="ruvX"
                     /locus_tag="VFZ80_01380"
     CDS             complement(1212..1661)
                     /gene="ruvX"
                     /locus_tag="VFZ80_01380"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX6286111.1"
                     /translation="MTRILGVDFGTKRIGLAISDDLGITARPLAVVARSEIRDAIERI
                     LDEYDIDRVVVGLPTGLGGKEGNSAESARDLGSEVEKLFGLTVEYVDERFTSRMAESA
                     LLESGMKRRDRKEHVDKVAAAIILQTYLDASAGNSPTDEDVEPPGIQ"
     gene            complement(1658..>2826)
                     /locus_tag="VFZ80_01385"
     CDS             complement(1658..>2826)
                     /locus_tag="VFZ80_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEX6286112.1"
                     /translation="DKTPFYAESGGQVGDSGRIVTDTGNAVVTDTQHVIQGLHGHKVK
                     VVEGSLRTGQPVRSAIDSPRREGIRKSHTGTHVLHYAIRDVLGDHANQAGSLVDDGRV
                     RFDFSHFSQVAPQELGDIEAEINRRLVDNSRVTTTVTTKEEAEAMGALAFFGDKYGEV
                     VRVVKVGEFSTEFCGGTHTTTSAQVGPLLITSESSIGSNLRRVEALTGMAAYDHLVSL
                     RRTLDKTGGLLRAPADQIPQRVEQLLSKVAGLEEKLEAIASQRMSSMAAELASGAERI
                     GDTALVVADAGDSDGNELRQIALGVRDHLDGPSAVVLGSSSGGKGALVGVLSKDLVGR
                     GLSAGDLLTSAASKLGGGGSRDPELAQAGGPRGDALGAALDSARDEMSRALAEL"
BASE COUNT          498 a          908 c          896 g          524 t
ORIGIN      
        1 gaggccggcg agatcgagca tcgtctcatg caggagcgga gacctggaat gttcgacggg
       61 atcgccgagc acggcgagcc gatagatggt cacgatgtcg attgtggcat cgagtcaggg
      121 aaggatcccg tcctcacggg cctgctccac gttggcgttg tgctcttcga gcgtctcggc
      181 gaaagcgtgg tgaccctcgg catccgacag cacgtagtag aagaacgggg tgtcatccgg
      241 ggcggccgcg gcctcgagcg acgcccgccc tggcgcagag atcggcgtgg ggggcagtcc
      301 gggatacagg taggtgttgt acggcgaatc aaaatctcgg ttgaattcgg caacgtccct
      361 cgtgcccaac gcgtacagga cggtggcgtc gatatcgagg gccatccctt cggcgagtct
      421 gttgtagatg acgctggaga cgatggggcg ctcttcgtcc aactggacct ccgattccac
      481 cagcgatgcc aggacgatcc cctgatacgg ggtgtatccg agatcctccc aagcgctcca
      541 gtctatcgag tcgaccctct gttccatggt cgaggcgaga cgctgcaaga tcgcgtcagg
      601 ctcggttgca gccgagaact cgtacgtgtc ggggaagagg agaccttccc agtcctggag
      661 ctcgggattc tccggcattt ccacgagaga agtcgtgaca ctgccgtcga gaagagccgt
      721 ctcgaagtcg ctgtactcgt gcggggtgtc ctcggccagt gaggtcagga tctcgctgac
      781 tctgagaccc tcgatgacgg tcacccgata gaccgatgcc cccggaccgg tgaccagcgt
      841 ggccacgacg tcggccggat ccatcaatgt caccagctcg taggtgccgg cctgaagcct
      901 ctgtgccgcc tcgacgttcc tcacggcgac ctcgaactcg agtgcggagc ggacaacacc
      961 ttgggcggcg aggatcgcac cgatgtcctg ggcggtagag ccgggatcga tctccacggt
     1021 cacttcgacg ccaggctcga catcgacagg cgcaccggtg acatcctggg ggccgagagc
     1081 atcgcccacc gtgcgcccca gataagaggc accggcgacg acgatcacag cgccgagcac
     1141 gatgccaacg ccgatgaaga gcaccttggt gatgcgggac cagcgtgatt ggtcgggaac
     1201 gggttcgaaa gtcattggat tccaggtggt tcgacgtctt cgtcggtcgg ggagttcccg
     1261 gccgatgcgt cgagataggt ctgcaagatg atggccgctg ccaccttgtc gacgtgttct
     1321 ttcctgtcgc gtctcttcat ccccgactcg aggagggcac tctcggccat tcgggaggtg
     1381 aatcgctcgt ccacatactc aacagtcaga ccgaagagct tttcgacctc ggatcccaga
     1441 tcgcgggccg attccgccga attcccctct ttcccaccga gcccggtggg caaaccgacg
     1501 acgacgcggt cgatgtcgta ttcgtcgagg attcgctcaa tggcgtctct gatctcgctg
     1561 cgggccacca cggccagggg gcgagccgtg atcccgagat cgtcggagat ggcgagcccg
     1621 atccgctttg ttccaaagtc gacccccagg atcctggtca caactcggcc agtgccctgc
     1681 tcatttcgtc ccgcgcggag tcgagagccg cacccaaggc atcacctctc gggccgcccg
     1741 cctgtgccag ctcgggatca cgtgacccgc caccaccgag tttcgaggca gccgacgtca
     1801 acaggtcacc agcgctcaac ccccgaccga ccaggtcctt ggagaggact ccgacgagcg
     1861 ctcctttgcc cccggaagag gagccgagca ccacggccga cgggccatcg agatggtctc
     1921 tgacgccaag tgcgatctgc cgcagctcat tcccgtccga atctccggcg tctgccacga
     1981 ccaaggcggt gtcaccgatt cgctccgctc ccgaggcgag ctctgcggcc atggagctca
     2041 tcctctgtga tgcgatggcc tcgagcttct cttccagacc ggccaccttg ctcagcagct
     2101 gctctacccg ctgcggaatc tggtcggccg gcgctcgcag caggcctccg gtcttgtcca
     2161 gcgtgcgcct cagcgagacg agatggtcgt aggcggccat cccggtcaag gcctcgaccc
     2221 ggcgcaggtt ggagccgatc gaggactcgc tcgtgatgag aagcgggccg acctgagcgg
     2281 acgtagtcgt gtgtgtccct ccacagaact cggtggagaa ctcgccgacc ttgaccacac
     2341 ggacgacctc gccgtacttg tcgccgaaga aggcgagagc gcccattgcc tccgcttcct
     2401 ccttcgtggt gaccgtcgtc gtcacccggc tgttgtcgac gagccgtctg ttgatctcgg
     2461 cctcgatgtc acccaactcc tgcggggcga cctgagagaa gtgtgagaag tcgaagcgaa
     2521 cacgcccgtc gtcgacgagt gacccggcct ggttggcgtg gtcccccaag acgtcgcgta
     2581 tcgcgtagtg gagcacgtgg gtgccggtgt ggctcttcct gatgccttca cggcgtgggc
     2641 tgtcgatcgc agatcgcacc ggctgaccgg tgcgaagcga gccttcgacc accttcacct
     2701 tgtgcccgtg gagaccctgg atgacgtgtt gggtgtcggt gaccaccgcg tttcctgtgt
     2761 cggtcacgat gcgaccggag tcgcctacct gaccacccga ctcggcatag aagggagtct
     2821 tgtcga
//
LOCUS       DASUTA010000072         2374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_542576, whole genome shotgun sequence.
ACCESSION   DASUTA010000072 DASUTA010000000
VERSION     DASUTA010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542576"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..666)
                     /locus_tag="VFZ80_01390"
     CDS             complement(109..666)
                     /locus_tag="VFZ80_01390"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6286113.1"
                     /translation="MKIAQEFVVARPPADVWTFFQDVPAVVQCLPGAELTDQNEDGSY
                     EGNLSVKLGPMSATFEGTATITPDEANRTATIEGKGVDKKGGSRGQVKVTYAITDAPE
                     GSRVNVDADVTLSGPAAQFGRTGLINEMSKRLIGDFVTCLEGKLGAASEEEADTYKAA
                     EVRGISLFFSSLWAWIKGLFGGSKK"
     gene            complement(724..1173)
                     /locus_tag="VFZ80_01395"
     CDS             complement(724..1173)
                     /locus_tag="VFZ80_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEX6286114.1"
                     /translation="MSDGVSAGAREDGSGEALESLLVGSGFDVTRDLVPDESDVIARA
                     ITRACSVADLVVTTGGTGFGLRDVTPEATGRVLEREAPGLVHAMVARGLEATPMAALT
                     RAKAGTVGHTLVINLPGSPKGAVENLEAILDVIPHALQLLSGDNKHH"
     gene            complement(1182..2027)
                     /locus_tag="VFZ80_01400"
     CDS             complement(1182..2027)
                     /locus_tag="VFZ80_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286115.1"
                     /translation="MTTGEATREEPGVVSRIGTPLIVASALASTFGIVVAQFLDDLGD
                     GLLAEFLGGDAVLYNNRVEFTGASDLAWGGGFVLCIVIGLFALFVYPTQRGHGVPRLV
                     LLWMLLHVLRQALTQAAFLPFQDDSQLALAYDTLDAPPGLDVVIAAGGGLGLLLIALA
                     AASAFLAFTPNRRLVSTPRRRTMLALWIALIPAVASAFAAIPYFLPDSQGLVLPGLPL
                     TAVIFLATLAAAPGTGSVTGPEDDRLITWPWGLVVFMILLLIFYLGVLQGGISVDPRL
                     WGTAQ"
     gene            2089..>2374
                     /locus_tag="VFZ80_01405"
     CDS             2089..>2374
                     /locus_tag="VFZ80_01405"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HEX6286116.1"
                     /translation="MSQEYAGVYVPGGSANVYESTSLANAGWYDEGQHGGAFAALIVG
                     HVEENVPTLTEMQVNRLTVEIFRVIPLVDLRIETDVLREGKRIQSVEAHVY"
BASE COUNT          456 a          747 c          767 g          404 t
ORIGIN      
        1 tgtcgacgac cacctcgtcg ccctcgattg cttcccctcc cccgtccgag ccaccacacg
       61 cgaccaaaag caacgcaccc gccgcggcga gcgccatcag acgtcttctc acttcttgga
      121 acccccgaaa agacccttga tccacgccca gagcgaggag aagaagaggg atatgcctct
      181 cacttcggcg gccttgtacg tatccgcctc ttcttctgaa gccgctccca gctttccctc
      241 caggcacgtg acgaagtcac cgatgagccg cttggacatc tcgttgatga gcccggtgcg
      301 cccgaactgg gccgccgggc ccgagagggt gacatccgca tcgacattca ccctggagcc
      361 ctcgggagca tcggttatcg cgtaggtcac cttgacctgg ccacgggaac cgcccttctt
      421 gtcgacaccc ttgccctcta tcgtcgcggt gcgatttgcc tcgtcgggcg tgatggtggc
      481 ggtgccctcg aaggttgcgc tcatcggccc cagcttgacg gacaggttcc cttcatagga
      541 gccgtcctcg ttctggtcgg tcaactcggc gccggggagg cactggacca ccgccggaac
      601 gtcctgaaag aatgtccaga cgtcggcggg cggtcgagca accacgaatt cctgtgcgat
      661 cttcatcgtt ctccagagag ttgggaagcg ggtcggaggc tagtgggtca ctcgccccaa
      721 ccttcaatgg tgcttgttgt ccccggacag gagttgcagg gcgtgcggga tgacgtcgag
      781 gatggcctcc aggttctcga cagccccctt cggcgagccc ggcaggttga tcacgagcgt
      841 atggccgaca gtccctgcct tggctctggt cagagcggcc atcggcgtgg cttcgagacc
      901 gcgcgcgacc atggcgtgaa ccagaccagg ggcctcgcgc tccagcaccc gccccgttgc
      961 ctccggagtg acatcgcgca acccgaaacc ggtgccgccg gtcgtcacga cgaggtctgc
     1021 aaccgaacag gcccgcgtta tcgccctggc gatgacatcg ctctcgtcag ggacgaggtc
     1081 tctcgtcacg tcgaacccgg acccgacgag cagactctcg agggcctcgc ctgagccgtc
     1141 ttcccgggct ccggcggaga cgccatcgct caccgtgatg actactgagc ggtgccccac
     1201 agcctcgggt caacggagat gccgccctga agcaccccca ggtagaagat caggagcagg
     1261 atcatgaaga caaccagtcc ccacggccag gtgatgagtc gatcatcctc aggtccggtc
     1321 acggagccgg ttcccggtgc tgcggcgagc gtggcgagga agatgacggc ggtcagtggg
     1381 agcccgggca ggacgagacc ttgactgtcg gggaggaagt agggaatggc ggcgaaggct
     1441 gaggcgaccg cgggtatgag ggcaatccag agtgcgagca tcgtgcgtct acgtggcgtc
     1501 gacaccagac gtcgatttgg ggtgaatgcg aggaaggccg acgccgcagc caaggcgatc
     1561 aggagcaggc cgagcccacc accggcagcg atgacgacat ccaaccccgg cggggcgtcg
     1621 agggtgtcgt atgcgagggc gagctggctg tcgtcttgga aagggagaaa ggccgcctgg
     1681 gtgagagcct gacgtaggac gtggagaagc atccaaagga gtacgagtct cggaacgccg
     1741 tggccccttt gggtcgggta gacgaagagg gcgaacaacc cgatgacgat gcacagcacg
     1801 aagccgcctc cccaagcgag gtcgctggct cctgtgaact cgacgcggtt gttgtagaga
     1861 acggcgtcgc caccgaggaa ctccgcaagc aacccgtctc cgaggtcgtc gaggaactgg
     1921 gcgacgacga tcccgaaggt ggaggcgagg gccgatgcaa cgatcagcgg tgttccgatc
     1981 cgggatacga ctcccggttc ttctctggtc gcttcacctg tcgtcacaag gtggtgatgc
     2041 taaacggaag atggccgacc gaaaggtctc ggttagcgtc caatgtcaat gagccaggaa
     2101 tacgccggtg tctatgtgcc gggcgggtcg gccaatgtct atgagtcgac ctccctcgcc
     2161 aatgccggct ggtacgacga aggtcagcac ggcggagcgt ttgccgccct catcgtcggc
     2221 cacgtcgagg agaacgtgcc caccctcacc gagatgcagg tcaaccggtt gaccgtcgag
     2281 atattccgtg tgatcccgct ggtcgatctt cgcatcgaaa cagacgtcct acgggagggg
     2341 aagcgaatcc aatccgtcga ggcccatgtc tacg
//
LOCUS       DASUTA010000073         2822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_412690, whole genome shotgun sequence.
ACCESSION   DASUTA010000073 DASUTA010000000
VERSION     DASUTA010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412690"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1426)
                     /locus_tag="VFZ80_01410"
     CDS             complement(<1..1426)
                     /locus_tag="VFZ80_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286117.1"
                     /translation="MSTEQMTPEDVALLGSGRHLIARQLRRAPKEFLFGGVGTTLYAG
                     MTILSSFVLGWIIDLMLIPAVSSGEVSTAALAASAGAIVAVATLRGIGITMRRTGAYY
                     AQHRLQYRDRVDVTDRYLDLPVEWHRRHPTGQLLANANEDVEAASFIAAPLPMAFGVV
                     LMLVGTGLILVLTDPFLALIGFFVGPVLIFVNYTFQRRMRAVAAEAQSLRAQVADIAH
                     ESFDAALVVKTLGREDDEVGRFGERSDALRDRMVEVGRLRASFDPLIEALPNLGILAV
                     LLVGAWRVDQGLLSAGTLVTFAYLFRLVALPMRVFGWLLGELPRSVVGLHRVEAVLNE
                     EDRVRYGSADPSVEGGAGAVVDAIAYQYPESEYQDLGAAVTTRQIANGDGRGIESVSL
                     DVTPGRTIAIVGPTGSGKSTVAHLMVRLFDPDTGQISLDGHTLVDLERNVLARSVSIV
                     FQETFLFNDTIYNNITLDEPFDDEE"
     gene            1574..1933
                     /locus_tag="VFZ80_01415"
     CDS             1574..1933
                     /locus_tag="VFZ80_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6286118.1"
                     /translation="MAKVRYIVSDVDEAITFYRDKLGFEVTMHPAPGFAALSRGDLTL
                     LLNQPGAGGAGKAGGDPEPGGWARFQLEVDDLESLVEQLQSAGVELKGEVVTGQGGKQ
                     ALALDPSGNLVELFQQA"
     gene            complement(1935..2408)
                     /locus_tag="VFZ80_01420"
     CDS             complement(1935..2408)
                     /locus_tag="VFZ80_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6286119.1"
                     /translation="MSQYVLQPADPREPDIVALLTTHLEFANQHSPPEHVHALDVEGL
                     SASDVSFYSLRDNGGLLAVGAIREIDPSHGEIKSMHTVQAARGRGIGRVMLDHLLEVA
                     RRRGYTRVSLETGTMEAFAPARRLYESAGFVVTPPFGPYWDNEYSVCMTLELVTS"
     gene            2476..>2822
                     /locus_tag="VFZ80_01425"
     CDS             2476..>2822
                     /locus_tag="VFZ80_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6286120.1"
                     /translation="MTDTVGAVRVIELNRPEVRNAVDAETASLLHEAFSRFDEDESVG
                     AAVLTGSGGNFCSGADLGVLDEEMNHRVQVAGPSPLGPARMSLSKPVVAGVEGFAVAG
                     GLELAIWCDLRVAG"
BASE COUNT          557 a          816 c          951 g          498 t
ORIGIN      
        1 cctcctcatc gtcgaaaggc tcgtccaggg tgatgttgtt gtagatcgtg tcgttgaaga
       61 ggaacgtctc ttgaaagacg atcgataccg acctggcgag cacgttccgc tcgaggtcga
      121 cgagcgtgtg cccatcgagg ctgatttgcc cggtatcggg gtcgaagaga cgcaccatga
      181 gatgagcaac ggtcgacttg ccggatccgg tgggcccgac gatggcgatg gttctgcccg
      241 gtgtcacgtc gaggctcacc gactcgatgc cgcgtccgtc accgttggcg atctgacggg
      301 tggtcacggc cgcaccgagg tcctggtact ccgattcggg gtattggtac gcaatcgcgt
      361 ccacgactgc gcccgcccca ccttcgaccg aagggtcggc cgagccgtat ctgactcgat
      421 cctcttcatt gaggaccgcc tcgacacgat gaagcccgac cacggaacga ggaagttcgc
      481 cgagaagcca tccgaacact cgcatcggga gagcaaccag tcgaaatagg taggcgaagg
      541 tcaccagggt tcccgcgctg agcaggccct ggtcgacccg ccaagccccg accagcagga
      601 ccgccaggat tccgaggttg gggagagcct cgatcagtgg gtcgaaagag gcacgaagac
      661 gcccaacctc gaccatgcga tcgcgcaggg cgtcggaacg ctctccaaac ctgccgacct
      721 cgtcgtcctc acgtcccagc gtcttcacca cgagggcggc atcgaacgat tcgtgggcga
      781 tgtcagccac ctgggcgcgc aggctctggg cttccgccgc caccgcgcgc atccgacgct
      841 ggaacgtgta gttgacgaag atcaacaccg ggccaacgaa gaatccgatc agagcgagaa
      901 acgggtcggt gaggacgagg atcagcccgg tgccaacgag catgagcacc accccgaagg
      961 ccatgggcag gggagcggct atgaacgaag ccgcttcgac gtcctcgttg gcgttggcca
     1021 ggagttggcc cgtcgggtga cggcgatgcc actcgacggg caggtcgaga tagcggtcgg
     1081 tcacatcgac ccggtcccga tactggagac gatgttgggc gtagtaggcg ccggtgcgtc
     1141 tcatggtgat gcctatgcca cggagagtgg cgacggcgac gatggcgcct gccgatgcgg
     1201 caagtgcggc cgtggagacc tcgccagagc tcacggcggg gatcaacatc agatcgatga
     1261 tccagcccaa gacgaatgag gagaggatgg tcatcccggc gtacaacgtc gttcccacac
     1321 ctccgaacag gaactccttg ggagctcgtc tcagctgtct ggcgatgagg tggcgtccgc
     1381 tgcccagaag cgcgacgtct tcaggtgtca tctgttcggt ggacatgagc tatctgaggg
     1441 tatgggggtc ctggataatg cggcaattga attccgcagg cggttcacgc taaaacctca
     1501 acatcagttg aagtcaagtt tctcggccgt cgatccgggt gaaaagtggt agacaccaat
     1561 caaggggact ctcatggcca aggtgcgata catagtcagc gatgtcgacg aggcgatcac
     1621 cttctacagg gacaagctgg ggttcgaagt caccatgcat ccggcacccg gattcgccgc
     1681 attgtcgaga ggtgatctga ctctccttct caatcagcct ggggccgggg gagcgggtaa
     1741 ggcgggaggt gaccctgagc cgggaggctg ggcccgattt cagctcgagg tggacgacct
     1801 cgaatcgctg gtggaacagc tccagtcagc cggcgtggag ctgaagggcg aggtcgtgac
     1861 gggacaagga ggcaagcagg cactagccct cgacccctct ggcaacctgg tcgagctgtt
     1921 ccaacaggcg tagatcagct cgtcaccagc tcgagtgtca tgcacacgct gtactcgttg
     1981 tcccaatagg gcccgaatgg gggcgtgacc acgaaaccgg ccgactcata gaggcgacgg
     2041 gctggtgcga acgcctccat ggtgcctgtc tccagactca cccgggtata gccgcgacga
     2101 cgtgccacct cgagcaaatg gtcgagcatg acccgaccga tgccgcgccc tcgggcagct
     2161 tggaccgtgt gcatcgattt gatctcgcca tggctcgggt cgatctcccg gatggcaccg
     2221 accgccagca ggccaccgtt gtcccggagg ctgtagaagg agacgtctga ggctgataga
     2281 ccctccacgt cgagggcgtg gacatgctcg ggtgggctgt gctggttggc gaactcgaga
     2341 tgtgtcgtga gtagggcgac gatgtcgggc tctctcgggt cggctggctg gagcacgtac
     2401 tgggacatcg gggcgaattt agcggtgcgc ccttacgctc tcgagcacgt cagaaggaag
     2461 gtgaacatga aggtggtgac tgacacggtc ggcgcggtgc gggtgatcga gctcaatcga
     2521 cccgaagtcc gcaacgcagt cgatgcggag acggcttcac tgctgcatga agccttctcg
     2581 agattcgacg aagacgagtc tgtcggggcg gcggtgttga ccggttccgg aggcaacttc
     2641 tgctctggag ccgatctcgg ggtcctggat gaggagatga accaccgggt ccaggttgcg
     2701 ggccccagcc cacttgggcc ggcgagaatg tcgctcagca aaccggtcgt agccggggtg
     2761 gagggcttcg ctgtggctgg agggctcgag ctcgccatat ggtgcgactt acgggtggca
     2821 gg
//
LOCUS       DASUTA010000074         2106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1321150, whole genome shotgun sequence.
ACCESSION   DASUTA010000074 DASUTA010000000
VERSION     DASUTA010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321150"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..545)
                     /locus_tag="VFZ80_01430"
     CDS             complement(198..545)
                     /locus_tag="VFZ80_01430"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009406067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX6286121.1"
                     /translation="MLRGEIRLVDFGQALPGESREQRPAVIVSNDSANSTAARLGRGV
                     ITVVPMTSTVTRVYPFQVFVPAHESGLDLDSKIQAEQVRSVSVHRVGRTLGSVPAPLM
                     MEVDEALRLHLLL"
     gene            complement(545..763)
                     /locus_tag="VFZ80_01435"
     CDS             complement(545..763)
                     /locus_tag="VFZ80_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015608544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX6286122.1"
                     /translation="MKISVSLPDEDVEFLDSYAQSHGYESRSAVVHTAVRMLRSSKLG
                     DAYAEAWLEWQESDDAELWDKTTSDGLT"
     gene            complement(916..>2106)
                     /locus_tag="VFZ80_01440"
     CDS             complement(916..>2106)
                     /locus_tag="VFZ80_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286123.1"
                     /translation="SGPVPSDYYLVSAADRAMAALDETSSVAFQAIVELEGAIENDAL
                     AMAWARLSALHPILTCVRAGDRWQPSQQPPFGEEAMPTGRQQTPVAMLVTPNGDGARL
                     TMMCNHVAFDGVASRILLGDLRAQYLAVLAGHPEPRPDMGARTLEAIAQAPDFRTSVA
                     TALRGSAAWWQTPVSTHADPAPPSSAAAEEHVVFELGPILDELTPLRRKHGWSVDAVL
                     VGILEKAWSQVFGSPRAESSWLVARDMRAGLGISGGIGNLSAAAGVSLSDPRGDLVDV
                     VDEAERILASQSHDLVTAASSLGRWGRVADMSFAQMLRRAKMLRAHRTVSNVGQLGGS
                     LDNWGQARLRRLWFVGPLAHPPFTSFIATGNGASTLVSVRVSPGWLTAEHAQAIEREA
                     SRLV"
BASE COUNT          398 a          671 c          685 g          352 t
ORIGIN      
        1 tggtcacctg tgcccgggtg ctggccgagt gggtgaccca ggagctatcg gttcccaact
       61 agtgcccggg ccagctgtct tcggagaaca cgctgatcca gattggaagg cgatcgatgc
      121 ctggcttcgg gagtaagtcg acgagtccta gtgagttgac aggtgaggtg gcagctccct
      181 tccagatttc gctcgtctca gagcaatagg tgaagcctca gagcctcgtc cacctccatc
      241 atgaggggtg ccggaaccga gccaagggtc cgcccgactc tgtggacaga gaccgaacga
      301 acctgctccg cctgaatctt tgagtccagg tcgagcccgc tctcgtgggc cggaacgaag
      361 acctggaacg ggtacacccg agtgacagtg gaagtcatcg gaacgacggt gatgacgccc
      421 ctcccgagtc gagccgccgt ggaattggca ctgtcattgc tcacgatcac ggcaggcctc
      481 tgttcgcggg attcaccagg cagcgcctgg ccaaaatcga ccaggcggat ctcgccgcga
      541 agcatcaagt caggccgtca gatgtggtct tgtcccagag ctctgcgtcg tctgactctt
      601 gccactcgag ccaggcttcg gcataggcgt ctccgagttt tgacgagcgc agcatgcgca
      661 ctgcagtgtg gacaaccgct gatcgagact cgtaaccgtg agattgggcg tagctgtcga
      721 ggaactcgac atcctcatca ggaagactga cactgatctt cacactgcaa gcctacccaa
      781 ggtagtacat gttggctacc acctcgcttc atcggcgcaa tcggtatcga gcgcaggtgc
      841 gaacgctcgc ggctcgccag gtagactcgc cgaccggccc attgtcaggg gtggacgacc
      901 gccgccgatc gaggctcaga cgagacgcga cgcctcccgt tcgatcgcct gcgcatgctc
      961 ggctgtcagc cagccgggcg agactctcac cgaaaccagg gtcgatgcgc cgttgcccgt
     1021 ggcgatgaaa gaggtgaaag gtggatgagc gagagggccc acgaaccaca agcgtcggag
     1081 gcgagcctgc ccccagttgt cgagggaccc gcccagttgc cccacgttgc tgaccgtccg
     1141 atgggcacga agcatcttgg cccgcctcag catttgtgcg aaggacatgt cagccacccg
     1201 gccccatcta cccagagatg acgccgcggt gaccaggtcg tggctctgcg aggcaaggat
     1261 ccgttcggct tcgtcgacga catcgaccag gtcgcctcgg ggatcactca gggagacacc
     1321 ggcggcggcg ctcaggttgc cgatgccccc cgagatgccc aatcctgccc gcatgtcgcg
     1381 ggcgaccaac caggagctct cagcccgggg agagccgaag acctgagacc acgccttctc
     1441 gaggatcccg accaggaccg cgtccaccga ccagccgtgc tttcgccgca gcggagtcaa
     1501 ctcgtcgagg atcggcccga gttcgaagac gacatgctcc tcggccgcag cgctcgacgg
     1561 aggagccggg tcggcgtggg tggagaccgg ggtttgccac catgccgcag aaccgcgcag
     1621 agccgtggcg accgacgtcc ggaagtcggg cgcttgggcg atagcctcca aggtgcgggc
     1681 tcccatgtcg gggcggggct cggggtgccc ggctagaaca gcgaggtact gcgctctcaa
     1741 atcgccgagg aggatccggc tggccacgcc gtcgaaggcc acgtggttgc acatcatcgt
     1801 aagccgcgca ccgtccccgt ttggggtgac gagcatggcg accggcgtct gctgccggcc
     1861 ggtcggcatc gcttcctcgc cgaacggcgg ttgttgagac ggttgccatc ggtcgcccgc
     1921 ccgcacacag gtgagaatgg gatggagagc ggaaagcctc gcccaagcca tggccagagc
     1981 atcgttctcg attgcgcctt ctagctccac gatggcctga aaggccaccg acgaggtctc
     2041 gtcgagggca gccatcgccc gatcggctgc cgacacgagg tagtagtcgg agggtaccgg
     2101 gccact
//
LOCUS       DASUTA010000075         3125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1709170, whole genome shotgun sequence.
ACCESSION   DASUTA010000075 DASUTA010000000
VERSION     DASUTA010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709170"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VFZ80_01445"
     CDS             complement(<1..367)
                     /locus_tag="VFZ80_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX6286124.1"
                     /translation="MTRRKADTWDRIKFLVLLGIIMTAIIASSPDLGFLSFGEVLGNF
                     FATTAGWVLGILFGLEVLRQIHYFISEKSAAYHSFWRDGVFGGMERFTHEHFKPWTRF
                     RVGRMFRWTFYLIILGVLLD"
     gene            complement(391..1026)
                     /locus_tag="VFZ80_01450"
     CDS             complement(391..1026)
                     /locus_tag="VFZ80_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286125.1"
                     /translation="MGRLTTLAIVSGLFLAGCGGTASDVRTVTDDESLSRFDLPEDWH
                     TYELSELSTLERLPFDAGYQNFTFPAISSIGFDGAPVSDVNQLTTSVADSDYPIGAAS
                     VRQIGDIERDFLSRAMLTQSVIPYASLPNPDELQKEDFTFGNGWEGVRLLVSFESDSG
                     SDLGVAYLISVSDPDDRRMYSIVAGCNRDCFIANQETITKVVDSWLVNKKG"
     gene            1261..1647
                     /locus_tag="VFZ80_01455"
     CDS             1261..1647
                     /locus_tag="VFZ80_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX6286126.1"
                     /translation="MPLLDLFWTMLWLFLFFLWIWLLISLFSDIFRRDDLGGWGKAGW
                     VFFLIVLPLLGALIYLIAEGGDMAERQVADARKMQAAQDEYIRSVAGGGGGASTADEL
                     EKLARLRDAGTITADEFAAQKAKLLG"
     gene            1763..2338
                     /locus_tag="VFZ80_01460"
     CDS             1763..2338
                     /locus_tag="VFZ80_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017583656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX6286127.1"
                     /translation="MADHKAPGYDLEPTLQFTEPDQYKALFEETRLQIIDLLTQRAAT
                     ITELADTLGKPKGTIGHHIGVLEQAGLIRVVRTKMVRAIEAKYYGRTARTYLLGDKHD
                     AGFGLAPDHFLTAAAAEFVNAPKDDPVGALSTLRHARIPTERADEWADRLMDLITEFT
                     EEPRGGETTYGLLVAMYPTNRPHLPDQEDED"
     gene            2335..>3125
                     /locus_tag="VFZ80_01465"
     CDS             2335..>3125
                     /locus_tag="VFZ80_01465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6286128.1"
                     /translation="MSKPLGVSYWKLWTASVVSNLGDGVSSVAYPWLASAITRNPLHI
                     AAVAVVTRLPWLLFSLPAGVVTDRVDRRKLAAWMDVFRFIFTLGVAFVVLASQSALAA
                     PGEIAAGTAAPPANAALLLVLVYAAAVLLGTAEVFRDNSAQTLLPAIVEKESLEKANG
                     RMWGAEMVMNSFIGPPLAGVLIGIAFSLPFFIDAGTFAVAAALVFLIAGEFTPKKDPD
                     EVSEVASSFWGDLKEGVRWLWGHPLFRPMAIALGILNGTMMMSLAT"
BASE COUNT          571 a          987 c          983 g          584 t
ORIGIN      
        1 agtcgaggag gacaccgagg attatcaggt agaaggtcca tcggaacatc cgaccgacac
       61 gaaaccgggt ccacggcttg aaatgctcgt gagtgaagcg ctccatgccc ccaaacacgc
      121 catcacgcca gaaggagtga taggccgccg acttctcgga tatgaagtag tggatctgac
      181 gcagaacctc gagaccgaac aggatgccga gcacccatcc tgcagtggtg gcgaagaagt
      241 tgcccagcac ctcgccgaaa ctgaggaacc cgagatcggg agagctggcg atgatcgccg
      301 tcatgatgat cccgagcagc accaggaact tgatccggtc ccaggtgtcg gccttgcgcc
      361 gtgtcacccg agcgtctctg tgtcttgcgt tcagcccttt ttgtttacga gccatgagtc
      421 gaccaccttc gtgatggttt cttggttggc gatgaagcag tcacggttgc acccggcgac
      481 gatcgagtac atgcggcgat cgtcggggtc ggacacggag atgaggtaag cgacgccgag
      541 atcggagccc gagtcgctct cgaacgacac gagaagacgg acaccctccc atccattgcc
      601 gaaggtgaag tcctccttct gcagctcatc ggggttgggg agactggcgt aggggatgac
      661 ggattgggtg agcatggccc gggagaggaa atcccgttcg atgtcaccga tctggcgaac
      721 ggaggcagcg cctatcgggt agtcggagtc ggcgacactt gtcgtgagtt gattgacgtc
      781 ggagacgggc gctccgtcga atccgatgct ggagatggca ggaaacgtga agttctggta
      841 ccccgcgtcg aaggggagtc gctccagtgt cgaaagctcc gacaactcgt aggtgtgcca
      901 gtcctcgggc aggtcgaaac ggctcaggct ctcgtcgtcg gtgacggtgc ggacgtccga
      961 tgcagtgccg ccacaccccg cgaggaagag gcccgacacg atggcgagcg tcgtgagtct
     1021 gcccatcctc ggcggcctca tcgctacaga ttacgacagg tttcgatttg gtggaacggt
     1081 cgcattccga acaggtaggc cagcccactg gagaggtccc tgccaccggc cctggtcttc
     1141 ggtgcgtccc cccaagcggc cccgcggcat ggctctttgc tttcggtcgt cctccgcccg
     1201 gggggcccgt ggcgaatgcc tagactcccg gggcaatcaa cctgattcga aggagaatca
     1261 atgccgttgt tggacctgtt ctggaccatg ctttggctgt tcctgttctt tctctggatc
     1321 tggctcctga tctcgctgtt ctccgatatc ttccgtcgtg atgacctcgg gggttggggc
     1381 aaggccggat gggtgttctt cctcatcgtc ctgccgctcc ttggagcttt gatctacctc
     1441 atcgccgagg gtggcgacat ggcggagcgc caggtcgccg atgcccggaa gatgcaggcc
     1501 gctcaggacg agtacatccg cagtgttgcc ggcggtggcg gcggagcctc caccgcggac
     1561 gagctggaga aactcgccag gctacgggac gccggcacca tcaccgccga cgagttcgcc
     1621 gctcaaaagg ccaagctgct cggttagaca cgacaatcaa cgaacccggg gatgccggcg
     1681 ccgtgtttgg cgtgaggaac cccgggttcg gtcgcgtaaa ggggttcttg acaaatggtc
     1741 gggttttgcc gactatacgg gaatggccga ccataaggca cccggttacg acctagagcc
     1801 cacactccag ttcaccgaac cggatcaata caaggcgctc ttcgaggaga ctcgactcca
     1861 gataatcgac ctcctcaccc aacgtgccgc caccatcacc gagctcgccg acacgctcgg
     1921 caagcccaag gggacgatcg gccaccacat cggtgtcctg gagcaagccg ggctcatccg
     1981 ggtggtccgc accaagatgg tcagggccat cgaggccaag tattacggcc gtaccgcccg
     2041 cacctacctc ctcggggaca agcacgacgc tggtttcggt ctcgccccgg atcacttcct
     2101 gactgctgca gccgccgagt tcgtcaatgc ccccaaggac gatccggtcg gcgccctcag
     2161 cacccttaga cacgcccgca tccccaccga acgcgccgac gagtgggcgg atcgactcat
     2221 ggacctgatc accgagttca ccgaggagcc gcgaggcggg gagaccacgt acgggctgct
     2281 cgtcgccatg tacccgacca atcggccgca tctgcccgac caggaggacg aggattgagc
     2341 aagccgctcg gcgtcagcta ctggaagctc tggaccgcct cggtcgtctc caatctgggt
     2401 gacggcgtgt cctcggtggc gtacccatgg cttgcctccg cgatcacccg caaccctctc
     2461 cacatcgcgg cggtcgccgt ggtcacccga ctcccctggc ttctcttctc cctgcccgcc
     2521 ggcgtggtga ccgaccgggt cgaccgtagg aagctggcag cgtggatgga tgtctttcgc
     2581 ttcatcttca cgctgggggt cgccttcgtg gtcctggcgt ctcaatccgc actggcggca
     2641 ccgggggaga tcgccgcggg gacggctgcg ccgccggcga acgctgcgct gctcctcgtc
     2701 ctggtctacg ccgccgctgt cctcctgggg acggcagagg tctttcggga caactctgcg
     2761 cagaccttgc tccccgccat cgtcgagaag gagagtctcg agaaggccaa cggtcgcatg
     2821 tggggtgccg agatggtgat gaactcattc atcggaccgc cgctcgcagg agtcctcatc
     2881 ggtatcgcct tctcactgcc cttcttcatc gacgcaggga cgtttgcggt cgccgcggcc
     2941 ctcgtcttcc tcatcgccgg ggagttcacg ccgaagaagg acccagacga ggtatccgag
     3001 gtggccagct cgttctgggg agacctcaag gaaggtgtcc gctggctctg gggacatccc
     3061 ctcttccggc cgatggcaat cgctctgggg atcctcaacg ggacgatgat gatgtcgctg
     3121 gccac
//
LOCUS       DASUTA010000076         2944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_285175, whole genome shotgun sequence.
ACCESSION   DASUTA010000076 DASUTA010000000
VERSION     DASUTA010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285175"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..1141
                     /locus_tag="VFZ80_01470"
     CDS             233..1141
                     /locus_tag="VFZ80_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286129.1"
                     /translation="MESFDKGAQQHAAGQRFLLTRSDSLELGGNDSGIRRRVDGSRWR
                     VVHPGVYQVDHRPPTWEDRLMASVLAAGVGALVSHRAALVLWGLDGISSAPVEITVPH
                     SHGPVPDGTIVHRTRRPADGTVIRHIPVTSVERTLLDGCSLLKHVPVAKAVESALRMR
                     LTSEERLWSHLKEHGGRGVKGTKQMRRVLRERQSDTATGSGSETEALLTLRSAGGIPE
                     PELQHRFTALDGTPIVPDFFWPKKNKAVEIDGIDAHDSADKLDHDLLRQNKLMDLGVE
                     LRRFSARHVRRRPDEFVRSVRRFLEI"
     gene            complement(1227..2039)
                     /gene="pucL"
                     /locus_tag="VFZ80_01475"
     CDS             complement(1227..2039)
                     /gene="pucL"
                     /locus_tag="VFZ80_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004846 - urate oxidase activity [Evidence
                     IEA];
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HEX6286130.1"
                     /translation="MPLGANRWGKSEVRVSKVFRGQVADDFIDLTVQVLLQGDVTAAH
                     TDGDNSSVLPTDTMRNTVYALAQDQLTRDLEAFGEALCDHFLERGDVERAEVALSERL
                     WQRGSPHGFIGGDSERRTARVTRGGMDSTKAGVDGLVVLKTSVSAFEGFPRDRFTILP
                     EASDRLLATSISAEWVYEAMPSNTSTTWALVRATLVDRFFEDWSASVQHQGYLMGEQV
                     LRSAPEVSQISLRLPNQHHLPFDLTRFDMPWSNVVFQPVSEPYGDIYLTVTR"
     gene            complement(2041..2355)
                     /gene="uraH"
                     /locus_tag="VFZ80_01480"
     CDS             complement(2041..2355)
                     /gene="uraH"
                     /locus_tag="VFZ80_01480"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEX6286131.1"
                     /translation="MTTLSTHVLDTASGEPARGMRVHLARFGEAGWEPVGELDTDDDG
                     RITGFGDLAGGIYRLGFETGEWGNDFLPFVHVVFTVDEERPHHHIPLLLSPFGYTTYR
                     GS"
     gene            complement(2355..>2944)
                     /gene="uraD"
                     /locus_tag="VFZ80_01485"
     CDS             complement(2355..>2944)
                     /gene="uraD"
                     /locus_tag="VFZ80_01485"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF010372.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEX6286132.1"
                     /translation="EPIHARYVRLSLHPDGGVARFRVLGRPDRDAAGALRLRYLNSLF
                     EEAARGFFFTTCVAKAWVEDMVAGRPYANIDEVLRRAATVFDTLSEDDWLQAFAGHPR
                     IGERGGETENREQAGTASASREVLRELVDVNRRYEEKFGFTYIVYATGKTASEMLSIA
                     KNRLDNDRSAEIAMAAAEQRKITETRLRRMLCQEA"
BASE COUNT          561 a          922 c          931 g          530 t
ORIGIN      
        1 atcaactgcg gcgccatcac cgaggacgag gccatgtccc gcaccgggct caccctcgac
       61 gagctccgca tccgctcctt tctcgccatc ctggagggga gacgggggta aggggagtct
      121 tggagccgtt ctgggcggat aatcgtccgg atcccggtcg attatccgcc cagaaggaac
      181 tgggaagctc gaggggattg aattcgcccg catatcgcat cacattcgag ctatggaatc
      241 cttcgacaaa ggtgctcagc aacacgccgc ggggcaacgt tttctattga cgagatcgga
      301 ttcgctcgag ctgggaggaa acgacagcgg gatcagacgt cgcgtcgatg ggagtcgatg
      361 gcgggtggtg cacccaggcg tgtaccaggt cgaccatcga cctccgacct gggaggaccg
      421 attgatggcc tcggtcctcg ctgccggcgt tggagccctg gtttcacata gagcggcgct
      481 agtgctctgg ggtctcgacg gaatctcatc agccccagtg gagatcacag tcccccactc
      541 ccacggacct gtgcctgacg gaaccatcgt gcatcgcaca agacgtccgg ccgacggcac
      601 cgtcatacgg cacatccccg ttacctccgt cgagagaaca cttctggatg gctgctccct
      661 gttgaaacac gtgccggtgg cgaaagcagt agagagcgcc cttcgaatgc gactgaccag
      721 cgaagagcgg ctctggtcgc acctgaagga gcacggaggt cgtggagtga agggcacgaa
      781 acagatgcgg agagtcctca gggagcggca gagcgacact gcgacgggga gcggaagtga
      841 gaccgaagcc ctgctgacgc ttcgtagcgc cggaggcatc cctgagccgg agcttcagca
      901 tcggttcacg gctctcgatg gcaccccaat cgtgccggac ttcttctggc cgaagaagaa
      961 caaggctgtg gaaatcgacg gtatcgatgc tcatgactct gcggacaaac tcgaccacga
     1021 cctgctgcgc cagaacaagt tgatggatct cggtgtcgag cttcggcggt tcagtgctcg
     1081 acacgtgcgg cgcagacctg acgagttcgt gcggtcggtg cgccgtttcc tcgagatctg
     1141 attacttctg gggggatatt tgtccgtata tcggtcaaat atccgcccag aacggttacg
     1201 cagggttggg cagcctgcca gaaccgtcag cgggtgacgg tgaggtagat gtcgccgtat
     1261 ggctcggaga cgggttggaa cacgacgttg ctccagggca tgtcgaaacg ggtgaggtcg
     1321 aaagggaggt ggtgttggtt ggggaggcgg aggctgatct gggacacctc cggggccgag
     1381 cgaagcacct gctctcccat cagatacccc tgatgctgca ctgacgccga ccaatcctcg
     1441 aagaagcggt cgaccagagt cgccctgacc agggcccacg tcgtggaggt gttggacggc
     1501 atcgcctcgt agacccattc ggcgcttatc gacgtcgcaa ggagacggtc cgaggcttcg
     1561 gggagaatcg tgaaccggtc cctggggaaa ccctcgaacg ccgagaccga tgtcttcagg
     1621 acgaccagac cgtcgacgcc cgctttggtc gagtccatcc caccgcgggt cactcgtgcc
     1681 gtccgacgct cggagtctcc gccgatgaac ccatgcggcg atccccgctg ccagagtcgt
     1741 tcgctcaagg ccacctcggc cctctcgacg tcgccccgct ccaggaagtg atcgcacaaa
     1801 gcctccccga aggcctcgag atcacgtgtg agctgatcct gggcaagggc gtacaccgtg
     1861 ttgcgcatgg tgtcagtcgg gaggaccgac gagttgtcac cgtcggtgtg ggccgctgtc
     1921 acatctccct gcaacagcac ctggaccgtg agatcgatga agtcatccgc aacctggcct
     1981 cgaaagacct tggaaacccg gacctccgac ttcccccacc ggttggctcc cagtggcacg
     2041 tcagctcccc cggtaggtgg tgtagccgaa cgggctcaac aacagcggga tgtgatggtg
     2101 ggggcgttcc tcgtcgacgg tgaagaccac atgcacgaat gggaggaagt cgttgcccca
     2161 ctcgccggtc tcgaagccaa ggcgatagat cccgccggcg aggtcgccaa aaccggtgat
     2221 gcggccgtcg tcgtcggtgt ccaactcgcc cacaggttcc caaccggcct cgccgaacct
     2281 ggcgaggtgg accctcatgc cccgcgccgg ctcaccactt gccgtgtcga gaacatgtgt
     2341 gctgagggtc gtcatcaggc ttcctgacac aacatacgcc ggagcctggt ctcggtgatc
     2401 ttgcgctgct ctgcggccgc catggcgatc tcggcgcttc gatcgttgtc gagccggttc
     2461 ttggcgatcg acagcatctc gcttgccgtc ttacccgtgg cgtagacgat gtaggtgaac
     2521 ccgaacttct cctcgtacct ccgattgacg tccacgagct cacgtaggac ctcccgtgac
     2581 gccgacgccg ttcccgcctg ctcccggttc tccgtctccc cacccctctc gccgatgcgg
     2641 ggatgcccgg caaatgcctg caaccagtcg tcttcgctca gcgtgtcgaa cacggtcgcg
     2701 gcgcgacgga gcacctcgtc gatgttcgcg tagggacgac cggcgaccat gtcctccacc
     2761 cacgccttgg cgacacatgt ggtgaagaag aagccccggg cagcctcctc gaacagcgag
     2821 ttgagatagc ggagacggag ggcaccggcc gcgtcccggt cgggacgccc gagaaccctg
     2881 aatctggcca cccctccatc ggggtgaagt gagagacgca cgtatcgggc atggatcggc
     2941 tcag
//
LOCUS       DASUTA010000077         4534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_933020, whole genome shotgun sequence.
ACCESSION   DASUTA010000077 DASUTA010000000
VERSION     DASUTA010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933020"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..738)
                     /gene="recO"
                     /locus_tag="VFZ80_01490"
     CDS             complement(22..738)
                     /gene="recO"
                     /locus_tag="VFZ80_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX6286133.1"
                     /translation="MAIVHDQGIVLRSYPFGEADRVVVLLSPNNGKIRTVAKGVRKTK
                     SRFGGRLEPFTHVDLVLYEGRNLDTITQVAVLEPFPRLREDLDAVVAAGTMVEAADAV
                     AQEDESAVRLFLLLQRGLKTLESGGRSPDLITSYLLKLADVVGVAPSLLQCASCGRVE
                     GLHRFSFSGGGSICDSCNVEGAVRLRDGVTTYLAALAGSELMALPPTDAAFSSEAMGI
                     ARRFVEYHLDRKLTSLAVME"
     gene            780..1058
                     /locus_tag="VFZ80_01495"
     CDS             780..1058
                     /locus_tag="VFZ80_01495"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003936052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX6286134.1"
                     /translation="MALLPDEQIQSFTEENADWKWSDSEITRTFEFEDFNEAMGFVTR
                     VALEAEKANHHPDIDIRWNKVTLTLSTHSEGGITEMDLDMATTADSLV"
     gene            complement(1116..1904)
                     /locus_tag="VFZ80_01500"
     CDS             complement(1116..1904)
                     /locus_tag="VFZ80_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6286135.1"
                     /translation="MDTDRYEYLHVDLQDDGVLLVTMGNEPRLNAVTVAGHRELVEIW
                     PRIDASADVRAVLLRSKGRAFSAGGAYEMIDEIMEDQSAHRRVLKEARDLVRNIIECS
                     KPIVSAINGPAVGAGLAAALLADIPVAGRAARIIDGHTNIGVTAGDHAAVIWPLLIGL
                     AKAKYYLMTNEPLTGEEAERIGLVAKVVDDEELEHFTLELTRSLAAGPTEALSWTKHT
                     LNHWLRTAYPIFDASVAYEFLGFHTADAAEGMSAVKEKRPPKFS"
     gene            1939..2667
                     /locus_tag="VFZ80_01505"
     CDS             1939..2667
                     /locus_tag="VFZ80_01505"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008387036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6286136.1"
                     /translation="MRYENKRAIVTGAASGIGAATTRLLAAEGAVVAALDIDVEGVAA
                     VAEEVGGTAIEVDLSDVDSLPTLVDDLMGELQALDLLVNCAGWDRAMPFVETDSAFWR
                     KVIDINLMGPIGLTHAVLSHLQEGGAIVNVSSDAGRVGSSGEVVYSGAKGGIIGFSKG
                     LAREVAGRGIRVNVVAPGPTDTPFLSGFDPTGKLAEAMMRQTPLKKLATPEDVASAIA
                     FLGSADAGHITGQVLSVSGGLTMV"
     gene            complement(2644..3543)
                     /gene="era"
                     /locus_tag="VFZ80_01510"
     CDS             complement(2644..3543)
                     /gene="era"
                     /locus_tag="VFZ80_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX6286137.1"
                     /translation="MKSGFVAVVGRPNVGKSTLVNRMVGSKVSIVSSRPQTTRNAIRG
                     VVHGDGFQIVLVDTPGLHKPKTELGSRLNSLVYGSLAESDAVLFIVDATMPIGPGDRL
                     IAQRLVDSNADVLVAVNKVDAASRQQTVAQLVEAAEWPFQHYFPISAVTGEGVPELVD
                     EIVSRLPEGPQFYPEEMTSDQPEALVIGEIVREKFLDRLRDELPHALVVRVEDMETRD
                     DGVLDIAADVIVERPSQKGIVIGRGGSLLKEAGTEARVELETILGTGVNLMLHVEVEK
                     DWQRSPQLLDRLGFEASDHGETA"
     gene            complement(3590..>4534)
                     /locus_tag="VFZ80_01515"
     CDS             complement(3590..>4534)
                     /locus_tag="VFZ80_01515"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX6286138.1"
                     /translation="VADTGSGWETLAVAVGLGILSFLIGDLIPRRLGRLNPEGLAYAS
                     QGMLRRAVRLGDWANDLIPDVENGDDTSEEDEDEESDEQERELIDSVLEFSETIVREV
                     MTPRPDMVTIGVTGTIQELIEISTSEGFSRVPVTSDGDVVGMVIVKDLLRMLTDTDGR
                     PQSVAQVMRPVEFVPETKLAAALLSEMQDTHTHQVIVVDEYGDVAGLVTIEDLLEELV
                     GEIADETDLEEDMVIPLADGWWVDARLSVAELSKATGVDVFEFEGDTVGGLILGLAER
                     VPEEGEVFAFERLALTVSRMQGRRVAEVNVAVTERVDT"
BASE COUNT          870 a         1458 c         1377 g          829 t
ORIGIN      
        1 cgacattgct caggtcgagg gtcactccat caccgccaag gaggtcaact tgcgatcgag
       61 gtggtactcg acgaaacgcc gggctatccc catcgcctca ctggagaacg cagcatcggt
      121 gggagggaga gccatcagct cagacccggc gagggcggcc agataggtgg tgacgccgtc
      181 gcgaaggcga accgcaccct cgacgttaca gctatcgcag atcgaccctc cgccggagaa
      241 cgagaagcga tgcagaccct cgacccggcc gcagctggca cattgcagga gagacggcgc
      301 aactcccacg acatcggcca gcttcaagag atacgaggtg atcaggtcgg ggcttcgccc
      361 tcccgactcc agggtcttca gtcctcgttg cagcaacaga aagagccgaa ccgcggactc
      421 gtcctcctgg gccaccgcat cggccgcttc gaccatggtc ccggcggcga ccactgcgtc
      481 gaggtcttcg cggagacgag gaaacggctc gaggactgcc acctgggtga tcgtgtcgag
      541 attgcgtccc tcatagagaa cgagatcgac atgggtgaaa ggctcgagcc tgcccccaaa
      601 acggctcttc gtcttccgca cgcccttggc caccgtgcgg atcttcccgt tgttcgggct
      661 caacaaaacg actactcgat cggcttcacc gaacgggtaa gagcgaagga ctatcccctg
      721 gtcgtggacg attgccatga aacgacaggg tattcagaga ggtgcgtagt ctcagtcaca
      781 tggcactact tcccgacgaa cagatccaat cgttcaccga ggagaacgcc gactggaagt
      841 ggtccgactc cgagataacc cgcacgttcg agttcgaaga cttcaacgag gcaatgggtt
      901 tcgtgacccg ggtcgccctg gaggccgaga aggccaatca ccacccggac atcgacattc
      961 gctggaacaa ggtgaccctc accctctcca cgcactccga aggcggcatc acggagatgg
     1021 acctcgacat ggcgaccacg gcggacagcc tcgtctgagc cgaagatgag ctgatttgag
     1081 ccgtatatgg cctgaggaac cccgggttcg cagtctcagg agaacttggg tgggcgcttc
     1141 tccttgacgg cggacatgcc ctcggcggca tcggcggtgt ggaagccgag gaactcgtag
     1201 gccaccgagg cgtcgaagat cgggtacgcc gtgcgcagcc aatggttcag ggtgtgcttg
     1261 gtccagctca gcgcctcggt ggggcctgca gcaagactcc gcgtcaactc cagggtgaag
     1321 tgctccagct cttcgtcgtc gaccaccttg gccaccaagc caatccgctc cgcctcttca
     1381 ccggtcaacg gctcgtttgt catcaggtag tacttcgcct tcgccaggcc gatcagcagg
     1441 ggccagatga cggctgcgtg atcccctgct gtgactccga tgttggtgtg cccgtcgatg
     1501 attcgagcgg cgcgaccggc gaccgggatg tcggccagca gggccgcggc caaacctgcc
     1561 ccgaccgccg ggccgttgat ggccgagacg atcggtttgg agcactcgat gatgttgcga
     1621 actaggtcac gcgcctcctt gagcacgcgt cgatgcgcag actggtcctc catgatctcg
     1681 tcgatcatct cgtaggcgcc cccagcggag aaggcccgcc ccttagatct gaggagcacc
     1741 gcacgaacat cggctgaggc gtcgatccgg ggccagatct ccaccagctc gcggtgcccg
     1801 gcgacggtca cggcattgag gcggggctca ttgcccatgg tgaccaacag gactccgtcg
     1861 tcttgcagat ccacatggag atactcatag cggtcggtgt ccatcacgtg acagtagatc
     1921 aaaggcgata atcgcggcat gaggtatgag aacaagcggg ctatcgtcac cggcgccgcc
     1981 tcgggcatcg gggcggccac gaccaggctg ctggccgccg aaggagcagt ggtggcggca
     2041 ttggacatcg atgtcgaggg agtggctgct gttgccgagg aggtcggagg cacagcgatc
     2101 gaggtcgatt tgagcgacgt tgacagcctt cccactctgg tcgacgacct catgggtgag
     2161 ctgcaggcgc tcgatctctt ggtcaattgc gccgggtggg atcgggcgat gccgttcgtc
     2221 gaaaccgact cggctttctg gcgcaaggtg atcgacatca acctcatggg gccgatcggt
     2281 ttgacacatg ccgtcctctc tcatcttcag gaggggggcg ccatcgtcaa cgtgtcatcc
     2341 gacgccggac gggtcggatc cagtggcgaa gtcgtctact ccggcgccaa gggcggcatc
     2401 atcgggttca gcaagggcct ggcccgtgaa gtggccggtc ggggcatcag ggtcaacgtc
     2461 gttgcccccg ggcccaccga caccccgttt ctctccgggt tcgacccgac cggcaagctc
     2521 gccgaggcga tgatgcgtca gactcctttg aagaagctgg ccacccctga agacgttgcc
     2581 tcggccattg cattcctggg gtcggccgat gccggtcaca taaccgggca agtcctatcg
     2641 gtgtcaggcg gtctcaccat ggtctgaggc ctcgaacccg agacgatcga ggagttgcgg
     2701 agaccgctgc caatccttct cgacctcgac atggagcatc aagttgactc cggtgcccaa
     2761 gatcgtctcg agctcgacac gggcctccgt tcctgcctcc ttcaggagac tgccacccct
     2821 tccgatgacg atccccttct ggctggggcg ctcgacgatc acgtcagctg cgatgtcgag
     2881 cactccgtcg tccctggtct ccatgtcctc cactctcacc acgagagcgt gaggcaactc
     2941 gtcacgcaac cgatccagga acttctccct gacgatctca cctatcacca gcgcctccgg
     3001 ctggtccgac gtcatctcct cggggtagaa ctgtggtccc tccggcagcc gtgacacgat
     3061 ctcatcgacc agttcgggaa caccttcgcc ggtcacggcc gagatcggga agtagtgctg
     3121 gaacggccac tccgctgcct cgaccagttg agccaccgtc tgttgccggc tggcggcgtc
     3181 gaccttgttc acagcgacca gaacgtccgc gttcgaatcg acgagacgtt gggcgatcag
     3241 acgatcacca ggtccgatgg gcatcgtcgc gtcgacgatg aaaagcacgg cgtccgactc
     3301 ggcaagcgat ccgtagacga gagagttgag ccggcttccc aactccgtct tcggcttgtg
     3361 gagcccgggt gtgtcgacaa ggacgatctg gaagccgtct ccatggacta cccccctgat
     3421 cgcgtttctc gtggtctggg gccgggacga gacgatggac accttcgatc cgaccatccg
     3481 gttgacgagt gtcgacttgc cgacgttggg gcgaccgacc accgccacga agccggactt
     3541 cactcgatga ccgccggcac gggtacggac tcacgtgaga cggcgagcac taagtgtcga
     3601 cccgctccgt cacggcaacg ttgacttcag ccacgcggcg gccttgcatt cgtgagacgg
     3661 tgagcgccaa tcgctcgaag gcgaagacct cgccttcctc aggcacgcgc tcggccagac
     3721 ccagaatgag gccaccaacg gtgtcgcctt cgaactcgaa gacatctaca cccgtggcct
     3781 tgctcagctc ggcaaccgag agccgagcat cgacccacca accgtcggcc agaggtatga
     3841 ccatgtcctc ttcgaggtcg gtctcgtcgg cgatctcacc gacgagctcc tcgagtagat
     3901 cctcgatggt caccaacccg gcaacgtcgc cgtactcgtc cacaacgatc acctgatggg
     3961 tgtgggtgtc ctgcatctcg ctcagcaacg ctgctgccaa cttggtctcc gggacgaact
     4021 cgacgggtcg catgacctgc gccaccgatt ggggtcgacc atcggtatcg gtgagcatgc
     4081 gcaggaggtc cttgacgatc accatgccga ccacgtcacc gtccgacgtg accgggactc
     4141 gtgagaagcc ctcagacgtc gagatctcga tcagctcctg gattgtcccg gtgactccga
     4201 tagtcaccat gtcgggccta ggagtcatca cttcgcggac aatggtctcg ctgaactcga
     4261 gaacggagtc gatgagctct cgctcctgtt cgtcactctc ttcgtcttca tcttcctccg
     4321 acgtgtcgtc gccgttctcc acgtcgggta tcaagtcatt ggcccagtcg ccgagtctca
     4381 cggcccgccg cagcatgcct tgagaggcgt aggcaaggcc ctcggggttg agtcgaccga
     4441 gacgacgagg aatcagatcg cctatcagga aggagaggat gcccaaaccc actgctaccg
     4501 cgagggtctc ccagccgctt ccggtatcgg cgac
//
LOCUS       DASUTA010000078         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_545314, whole genome shotgun sequence.
ACCESSION   DASUTA010000078 DASUTA010000000
VERSION     DASUTA010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545314"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VFZ80_01520"
     CDS             complement(<1..755)
                     /locus_tag="VFZ80_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX6286139.1"
                     /translation="MTRAITVIANDSLTDEQVEQAKSIFDKASVSVEWRATADSESRS
                     RIAAEAAAQGADVVIGFGGDGTVRACAEGVTGTDTLLGVIPAGTGNLLARNLGIPSDL
                     EESITVALGENVRRLDTGRANGELFAVIAGVGFDAEVMANTEDAAKDRVGSLAYFMQG
                     ARHLGDPSFEARITVAGADMANGDWATILVGNLSQLQGGVELFPDSSPDDGVLDVLGI
                     SADTPLERLAAGLEAALGDGMGEHLIRASGTEVT"
     gene            complement(752..1486)
                     /locus_tag="VFZ80_01525"
     CDS             complement(752..1486)
                     /locus_tag="VFZ80_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX6286140.1"
                     /translation="MREPMVDSAVESRIGGSSDLSSRWPLRSRAVLEIVAAFIALTLT
                     GLSAGWLILGPLGGSALAEWDREVAVWLAARRDPTLDTLTDIGSAFSNTENIVIGLLV
                     LMVAMVLVWRRWRESLTLGVALGLEASVFLAVSTIVGRDRPPVEQLDPSPPTASFPSG
                     HTGAAFAFYIGLAFIVFWTTRKRLPRAIGVAGATLVPVAVAVSRMYRGMHFMTDVVIG
                     AALGAASVLIAIVIVHRAVGRMRAKT"
     gene            complement(1483..>2249)
                     /locus_tag="VFZ80_01530"
     CDS             complement(1483..>2249)
                     /locus_tag="VFZ80_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX6286141.1"
                     /translation="PEIEKHWLVRLVSRSPRLSRFVQRRLDRTSAGGLLLTVGFIVVL
                     ALAVSVGVIFDMVDNETGFAHFDSAVAEFGARNADSFWWDIQSLFTRLGGTLVIALVA
                     LVVGLWGWWRQGTHHVFMFMFVVVVGQALLNSGLKLLVGRTRPDVLQLVPFSGSSFPS
                     GHSAAAAATYLAAAFVFAIGMRRHHRFVAIAAGSFLAAGVGATRALLGVHWLTDVLAG
                     LAVGFAWFIVCAVAFGGRLMYFGEPKYEVVAQGAEP"
BASE COUNT          446 a          734 c          699 g          370 t
ORIGIN      
        1 gtcacctcgg tccctgatgc tcgaatcaaa tgctccccca tgccatcccc gagagcggcc
       61 tccagtccgg cggccaacct ctcgagaggc gtgtcggcag agataccgag aacatcgagg
      121 actccgtcat cgggggacga gtcggggaac agctcgactc cgccctggag ctggctgaga
      181 ttgcccacca atatggttgc ccaatctcca ttcgccatgt cggcgcctgc caccgtgatc
      241 ctcgcctcga aggagggatc cccgaggtgg cgtgcgcctt gcatgaagta ggcgagagag
      301 ccgacccggt cttttgcagc gtcttcggtg ttggccatga cttcggcatc gaagccgaca
      361 ccggcgatca cggcgaagag ttcgccgttt gcccttccgg tatcgaggcg ccggacgttc
      421 tcaccgagag ccacggtgat cgactcctcc agatcgctgg gtatgccgag attgcgagcg
      481 agcaagttgc cggtgcccgc ggggatcact ccgaggagag tgtcggttcc ggtgactccc
      541 tcggcgcacg cccggacggt gccgtcgcct ccaaaaccga ttacgacatc tgcgccctgc
      601 gccgcggcct cagcggcgat tcgactcctg gattcggagt cggctgtcgc tctccattcg
      661 acagataccg atgccttgtc gaagatgctt ttcgcctgct cgacctgctc atctgtaagg
      721 gagtcgtttg cgatgacggt tatggcacgt gtcatgtctt ggctctcatt ctgcccacag
      781 ctctgtggac gatgacgatg gcgatcagaa cgctggctgc cccgagtgcg gctccgatga
      841 ccacatcggt catgaagtgc atccctcgat acatcctgga aacggccaca gcgaccggca
      901 ccaacgtcgc accggctacg ccgattgccc tggggagcct ctttcgagtc gtccagaaca
      961 cgatgaaggc cagtcctatg tagaaggcga atgctgcccc tgtgtgacca ctggggaagc
     1021 tggccgttgg cgggctgggg tcgagctgtt ccaccggtgg ccgatcccgc ccgacgatgg
     1081 tgctcaccgc gaggaagacc gaagcctcca gacccaaggc gacaccgagg gtcagggact
     1141 cgcgccatcg ccgccacacg agaaccatcg ccaccatgag gacgagaaga ccgatgacga
     1201 tgttctctgt gttcgagaat gccgaaccga tgtcagtcag tgtgtccagc gttgggtcac
     1261 gccgggcagc gagccagacc gcaacctctc gatcccactc ggccagtgca gatccgccca
     1321 atggtccaag gatcagccag cctgccgaca acccggtaag cgtcagcgcg atgaaggcgg
     1381 ccacgatctc cagcaccgct cgagagcgga gtggccatcg cgaggagagg tcggaagatc
     1441 cccctattcg cgattcgacg gcgctgtcca ccatcggttc cctcatggct ccgccccctg
     1501 cgccacgacc tcgtatttcg gctccccgaa gtacatgagg cgccccccga aggcgactgc
     1561 gcagactatg aaccaggcga accccaccgc caagccggcc agtacgtcgg tcagccagtg
     1621 caccccgagc agggcgcgcg tcgcgccgac acctgccgct aggaacgatc cggccgctat
     1681 cgccacgaat cggtggtggc gcctcatgcc gattgcaaac acgaaggcag cagcgaggta
     1741 ggtggcggct gctgcagcgc tatggccgga ggggaaagag gaccccgaga acgggacgag
     1801 ctgcagcaca tcgggccggg tcctccccac gaggagcttc aggccgctgt tcaagagagc
     1861 ctgacctacg acgacgacga acatgaacat gaagacgtgg tgggtgccct gtcgccacca
     1921 tccccatagg ccgacgacca atgcgaccag ggcgatgacc agggttccac ccagacgcgt
     1981 gaagaggctc tgtatgtccc accagaagga atcggcgttg cgtgcgccga actcggctac
     2041 ggcactgtcg aagtgggcga agccggtctc gttgtccacc atgtcgaaga tcacgcccac
     2101 ggacaccgcc agagccagta cgacgatgaa gccgaccgtg agaaggaggc cgcccgccga
     2161 agtccggtcg agccggcgct gaacgaagcg ggacaggcgg ggagatcgcg atacgagacg
     2221 gaccagccag tgcttctcga tctcgggat
//
LOCUS       DASUTA010000079         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_286065, whole genome shotgun sequence.
ACCESSION   DASUTA010000079 DASUTA010000000
VERSION     DASUTA010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286065"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VFZ80_01535"
     CDS             complement(<1..455)
                     /locus_tag="VFZ80_01535"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX6286142.1"
                     /translation="MKRIGFLALMTLLTVACLGSDFADSVEGSWQLSEGTLDGEPIPL
                     IDSHPITITFQDDQVGGTASCNSYGGTYETDGSTISFSSLAMTEMACMPEETMVAEQM
                     YGTALTMVDTVTLDESLVLSGPGVELTFDRLDPVPGAELTNTVWVLDGL"
     gene            complement(489..1373)
                     /locus_tag="VFZ80_01540"
     CDS             complement(489..1373)
                     /locus_tag="VFZ80_01540"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEX6286143.1"
                     /translation="MNRLEVWRTAARVHTLPAAVVPVLVGSGLAYGEEVFRWDAFSWA
                     LVGALAIQVAANFANDASDAKRGADTSDRLGPPRMVATGVVTSRQMWMAAWVAIAVAA
                     VAGIALIVIAGWLVLLIGASSIVAMLGYVGGPAPYGYRGLGEVFVFVFFGLVATVGSR
                     YVHDMTAPRAAWLLAIPVGMLVTAILVANNYRDIDTDRSAEKRTLAVVMGREATRVLF
                     AVLVLGSFAAIAVFAVAGWIPITTLFCGFLLPLAAVPIRIINTRTDGPALIRALKATA
                     RLHLWVGVILAAAVAFTL"
     gene            complement(1378..2268)
                     /locus_tag="VFZ80_01545"
     CDS             complement(1378..2268)
                     /locus_tag="VFZ80_01545"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HEX6286144.1"
                     /translation="MVSQIFDPSAWDPIPGFAFTDITYHRAKDTGVVRVAFDRPEVRN
                     AFRPHTVDELFEALDHARQSSDVGCVLLTGNGPSPKDGGWAFCAGGDQRIRGKDGYKY
                     ADGEEIDPGRTGRLHILEVQRLIRFMPKVVICVVPGWAVGGGHSLHVVCDMTLASEEH
                     AVFKQTDADVASYDAGYGSAYLARQVGQKRAREIFFLGRDYTAQEAHEMGMVNAVVPH
                     DQLESVALEWGREVVAKSPMAQRMLKFAFNMIDDGLVGQQIFAGEATRLGYMTDEAQE
                     GRDSFLEKRDPEFGRFPWHF"
     gene            2300..>2573
                     /locus_tag="VFZ80_01550"
     CDS             2300..>2573
                     /locus_tag="VFZ80_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286145.1"
                     /translation="MIEWLRRVDPDRPFLWAGERCWTYGDAVIEVEDRITSDYLVLGP
                     DLTPSAVFQVMAGLSGGGLTVGGPGGWVGPEPDADLVVFTSGTTGLP"
BASE COUNT          489 a          820 c          824 g          440 t
ORIGIN      
        1 atgagcccgt cgaggaccca gaccgtgttg gtcagctcgg cgccagggac agggtcgagt
       61 cgatcgaacg tgagctcgac gccggggccg ctcaggacga ggctctcatc gagagtcacg
      121 gtgtcgacca tggtcagagc cgtcccgtac atctgctctg cgaccatcgt ctcttccggc
      181 atgcacgcca tctcggtcat ggcgaggctc gagaaggaga tggtggagcc gtccgtttcg
      241 taggtgccgc cgtagctgtt gcacgatgcc gtaccaccca cctggtcgtc ctggaaagtg
      301 attgtgatcg gatgggagtc gatcaagggg atcggttcgc cgtccagtgt cccttcggag
      361 agttgccagg acccctcgac gctgtcggcg aagtccgagc cgagacaggc aacagtgagc
      421 aacgtcatta gggcaaggaa accgattctc ttcatgacga ttagacggat cgacattgcc
      481 ctccggtttc agagcgtgaa agcaaccgcg gcggccagga tcacacccac ccagagatgg
      541 agcctcgccg tcgccttgag agcccggatc aaggccggac cgtcggtccg ggtgttgatg
      601 atccgaatcg ggacagcggc gagcggtagg aggaaaccgc agaagagggt cgtgatcgga
      661 atccaccccg caacggcgaa caccgcgatc gcggcgaaag agccgagtac gaggaccgca
      721 aacagaaccc tcgtcgcctc ccgtcccatc actactgcca atgtcctctt ctcagccgat
      781 cggtcggtgt cgatgtcgcg gtagttgttg gctacgagga tggccgtgac cagcatgcca
      841 acagggatcg cgagcagcca tgcggcgcga ggagcggtca tgtcgtgcac gtatcgcgac
      901 cccaccgtgg caaccagacc gaagaacacg aagacgaaca cctctcccaa ccctcggtat
      961 ccgtaaggag cggggccgcc cacatacccg agcatcgcaa cgatcgagga ggcaccgatg
     1021 agcaggacga gccacccggc gatgacgatg agagcgatac cggcgacggc ggcgacagcg
     1081 atggcaaccc aggccgccat ccacatctga cgcgaggtga cgacgccggt ggcgaccatc
     1141 cgaggtggcc cgagccgatc ggatgtgtcc gctcccctct tcgcatcgga ggcgtcgttg
     1201 gcgaagttgg ccgctacctg gatcgccagt gccccgacca gtgcccatga gaacgcatcc
     1261 caacggaaga cttcctcacc gtaagcgagg cccgaaccga ccaggacggg gacgacggcc
     1321 gcgggcagag tgtggacacg ggccgccgtc ctccacactt cgagtcggtt catgcgctca
     1381 gaagtgccag gggaatcggc cgaactcggg gtcgcgcttc tccaagaacg agtctcgccc
     1441 ctcctgggcc tcgtcggtca tatatccgag tcgggtcgct tccccggcga agatctgttg
     1501 accgacgagc ccgtcgtcga tcatgttgaa ggcgaacttc agcatccttt gtgccattgg
     1561 gctcttcgcc accacttccc gtccccattc cagagcaacc gactcgagct gatcatgagg
     1621 cactacggca ttgaccatgc ccatttcgtg ggcctcctgc gccgtgtagt cgcggcccag
     1681 gaagaagatc tcccgggccc tcttctgtcc cacctggcgg gccaggtatg ccgatccgta
     1741 tccggcgtcg taggaggcga cgtcggcgtc ggtctgcttg aagacggcgt gctcctccga
     1801 cgcaagcgtc atatcgcaga cgacatggag gctgtgcccc cctccaaccg cccaccccgg
     1861 caccacacag atgaccacct tgggcatgaa gcggatgaga cgctgcacct cgaggatatg
     1921 gagacgcccg gttcgacccg ggtcgatctc ctctccgtcc gcatacttgt agccgtcctt
     1981 accccgtatc cgctggtcgc ctccggcaca gaaggcccat ccaccgtcct ttggcgaggg
     2041 gccgttgccc gtgaggagga cgcagccgac atctgagctc tggcgggcgt ggtcgagcgc
     2101 ctcgaagagc tcgtcaacgg tgtgggggcg aaaggcgttt cgcacttcgg gccggtcgaa
     2161 ggcgacccgg accacaccgg tgtccttggc gcggtgatag gtgatgtcgg tgaaggcgaa
     2221 gcccgggatc gggtcccagg cgcttggatc gaatatctga gaaaccacga agcgagagga
     2281 taaggggcac aatcggtctg tgatcgaatg gctgcgtcgc gtcgaccccg accgcccttt
     2341 tctgtgggca ggtgaacggt gctggaccta cggcgatgcc gtcatcgagg tcgaggacag
     2401 gatcacctcc gactacctcg ttctcgggcc cgacctgacc ccatctgcgg tgttccaggt
     2461 catggcgggc ttgtcggggg gcggtttgac ggtcggaggg cccggaggct gggtcggtcc
     2521 agagccggac gccgacctcg tggtgttcac ctcgggcacc accggtcttc cca
//
LOCUS       DASUTA010000080         3330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_934026, whole genome shotgun sequence.
ACCESSION   DASUTA010000080 DASUTA010000000
VERSION     DASUTA010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934026"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFZ80_01555"
     CDS             <1..194
                     /locus_tag="VFZ80_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6286146.1"
                     /translation="LAAAREADVTLPAVSLTHQLVQGLIGMGHGEDDFAALIIQQAVL
                     SGMELVAEDADVTDGLGDL"
     gene            194..1030
                     /locus_tag="VFZ80_01560"
     CDS             194..1030
                     /locus_tag="VFZ80_01560"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6286147.1"
                     /translation="MVDWAVVRSVEATTDESGVTDLVGAPQGAVHLDVALVTLAPGER
                     RDPEVSPFESSFFVLDGRVVLQLAGQLHDLGPDDFGYVPVATPHRWENRGDRSATYLR
                     VRSPQPRDFGVVQSGADLADLDQSVDPKPVDSSDPASRQVGHFSDEHLPDPGPLQMKG
                     FRSGTARNVGLWMLVDEVIGAVHHTLFMVQFAPTGQTVTLGGQHYHPFEEIYYIVEGQ
                     AVAHLEDQSIPVGVGDTVFAGVNSLHGFSNQSDSRVRWIEAQAPAPPPSGAFFFPSQW
                     SR"
     gene            1266..1664
                     /locus_tag="VFZ80_01565"
     CDS             1266..1664
                     /locus_tag="VFZ80_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX6286148.1"
                     /translation="MNFMFQRKTDLAVRALKYLGTPGVKRSGASLSSSIGTTTAFLPQ
                     VLAPLIDQGWVTSDRGPGGGYALTDASTQVSVLEVLEATQGPTVNGRCVLRDQPCPGS
                     EECDVHAAWTTAREVLVEGLRKIPVIETGE"
     gene            1666..3270
                     /locus_tag="VFZ80_01570"
     CDS             1666..3270
                     /locus_tag="VFZ80_01570"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX6286149.1"
                     /translation="MTQTINREEKTSSKDVPSHPHYTLGTLIIVGVLGTLVALGIAIA
                     ALMITASRNSVDDELVSERVDSLLAEAVLPSDLPEDVIPELVPKAELAPVPAGEINVA
                     VAPEMPPASGRTTPAIVEVEFEVVEGINAIDPATGVETETWGYRLADGPDSVVIGTPG
                     PVIRARVGDILRFTITNPADNANPHNVDFHAVTGQGGGAEATTVAPGETKTIEARLLY
                     PGFFMYHCAHGDIPSHISHGMYGGILVDPETPLPAVDHEWYMVQSEYYTASKDPGVVE
                     FDRQSVTDENPSYVVFNGAVGALTGDNALEMAVGERARIYFINAGLNLDSNFHPIGSH
                     WDRVYQEAALLNQPLRGSQTTLVPAGGGTVVEVIGQVPSTIVLVDHALSRAVDKGAIG
                     QIVVAGDPDLEIFEDVTGGGTGAEGGHDMGGGEGTEAPAGEQVSIVEGAWTTQDLGAA
                     DEFADTESPADYSVNVLTVPVGTTVTWSNDDPGMIHTVTSVDGQFDSGFLNEGDTWSY
                     TFDEPGEYEYLCTPHPWMRAKVVVEP"
BASE COUNT          598 a         1064 c         1054 g          614 t
ORIGIN      
        1 ggctcgccgc ggcccgcgag gcagacgtga cgctgcccgc ggtttcgctg acacaccaac
       61 tggtccaggg ccttatcggg atgggccacg gtgaagatga ctttgcggcc ctgatcatcc
      121 aacaggcggt gttgtcgggt atggagttgg tggcagagga cgccgacgtg accgatgggc
      181 tgggagacct ctgatggtcg attgggccgt tgttcgtagc gtcgaagcca ccaccgacga
      241 gtcgggggtg acggatctgg ttggtgcccc acagggagcg gtacatctgg atgtggctct
      301 cgtcacactg gctcccggag agcgacgcga ccctgaggtc agcccgttcg agtcgtcgtt
      361 cttcgtcctc gacgggcggg tggtgctcca gctcgccggg cagctgcatg acctcgggcc
      421 tgacgacttc ggctatgtgc cggtggctac tccacatcgg tgggagaatc ggggcgaccg
      481 gtcggccact taccttcgtg tgcggtcacc gcagccgcgc gatttcggag tcgtccagtc
      541 gggtgcagac ctggctgatc tcgaccaatc agtcgatccg aagccggttg attcctcgga
      601 tcccgcgtcg cgtcaggtag gacacttctc cgacgagcat ctccccgatc cgggcccact
      661 gcagatgaag ggtttccgct cgggaacggc gaggaacgtc ggcttgtgga tgctggtcga
      721 cgaggtgatc ggcgcagtcc atcacacctt gttcatggtt cagttcgccc ccaccggtca
      781 gacggtcacg ctcggtggcc agcactacca ccctttcgag gagatctact acatcgtcga
      841 gggtcaggca gtggcgcacc tggaggatca gagcattcca gttggtgtcg gggacaccgt
      901 ctttgccggg gtcaactcac tgcacggatt ctctaaccaa tccgacagtc gggtgcgctg
      961 gatcgaggcg caggctccag caccgcctcc gtctggtgcg ttcttcttcc cgagccagtg
     1021 gagtcggtag gaagccgtac acggggcttt gctgtctgac atgagcaact cgccttgcgt
     1081 aacggcgacg gtgtagcggg cccacggacc cacccgtctc gaccgcttgt cagccgtcga
     1141 acaactgctg cgccgggggt ggtctatcca cgcgccgacg tgtgctcgtt ggggccgaaa
     1201 gtcccgaaga cgagggacca acgactcttc ggctcgtaat tgttgagtag ttgactcagg
     1261 aactgatgaa tttcatgttc caacgcaaga ccgatctcgc cgttcgtgcc ctcaagtacc
     1321 tgggaactcc gggcgtgaag cgctccgggg cgtcgttgtc ctcttcgatt ggcaccacca
     1381 ctgccttctt gccgcaggtg ctggcgccgc tcatcgatca gggttgggtc acctccgacc
     1441 gtggtcccgg cggcggatat gccctcaccg acgcctccac ccaggtctca gtactcgagg
     1501 tcctcgaagc gacacaaggg ccgacggtga acgggagatg tgtcctgcgt gatcagccct
     1561 gccctggaag cgaggagtgc gacgtgcatg ccgcttggac gaccgcacgg gaggttcttg
     1621 tcgaagggct tcggaagatc ccggtgatag agacaggaga atgaaatgac acagacgatc
     1681 aatcgggagg agaagacgtc ctccaaggac gttccgtctc acccgcacta cacactcggc
     1741 actctgatca tcgttggcgt tctcggaacg ctcgtcgccc tcggcatcgc catagctgcc
     1801 ttgatgatca cggccagtcg caactcggtc gacgacgagc tggtttcgga gagagtcgac
     1861 agccttctcg ccgaggcagt acttccgagc gacctgcccg aagacgtgat tcccgagctc
     1921 gtgcccaagg cagagctcgc accggttccg gccggagaga tcaatgtcgc cgtggctcct
     1981 gagatgcctc cggcctccgg tcgcaccacc cctgccatcg tcgaggtcga gttcgaggtc
     2041 gtcgagggga tcaacgcgat cgatccggcc accggggtcg agaccgaaac ctggggatac
     2101 cgtctggctg acggtcccga cagtgtcgtc atcggcacac cgggaccggt gataagggcg
     2161 agagtcggag acatcctccg gttcacgatc accaacccgg cggacaacgc caacccgcac
     2221 aacgtcgact tccacgccgt caccggacag ggcggtggcg ccgaagcgac caccgtggct
     2281 cccggcgaga ccaagaccat cgaagcccgt cttctctacc cgggcttctt catgtatcac
     2341 tgcgctcacg gcgacatccc gtctcacatc tctcacggta tgtacggcgg gatcctcgtc
     2401 gacccggaga ccccgctccc tgcggtcgac cacgagtggt acatggtgca gtcggagtac
     2461 tacacggcga gcaaggatcc cggggtcgtc gaattcgatc gccagtcagt cacagatgag
     2521 aaccccagct acgtcgtctt caacggcgcc gtcggcgcgc tgaccggcga caacgccctg
     2581 gagatggcgg tcggtgagag agcacggatc tacttcatca acgccggtct caacctggat
     2641 tccaatttcc accccatcgg gtcgcactgg gacagggttt accaagaggc cgccctcctc
     2701 aaccagcctc tccggggttc gcagacgacg ctcgttcccg ccggcggcgg caccgtggtc
     2761 gaggtgattg gccaggtgcc ctccacgatc gtcctcgtcg accatgccct gagccgggca
     2821 gtggacaagg gagcgatcgg acagatcgtc gtcgccggtg accccgatct ggagatcttc
     2881 gaggacgtca ccggtggcgg caccggcgcc gaaggcggcc acgacatggg aggtggtgaa
     2941 ggcaccgagg cgcctgccgg cgagcaggtc tccatcgtcg agggcgcatg gaccactcag
     3001 gacttgggcg ctgccgacga gttcgccgat acggagtcgc ccgccgacta ctcggtcaat
     3061 gtcctcaccg tcccggtcgg caccacagtc acctggagca atgacgaccc ggggatgatc
     3121 cacacggtga cctcggtcga cggtcagttc gactcagggt tcctcaacga aggcgacact
     3181 tggtcgtaca ccttcgacga gcccggcgag tacgagtacc tctgcacgcc ccacccatgg
     3241 atgcgagcaa aggtcgtcgt cgagccctga ccccgcctga ggtagatgag gggccgtccc
     3301 atgtgttggg acgacccctc ctgttgtcgt
//
LOCUS       DASUTA010000081         2764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_157234, whole genome shotgun sequence.
ACCESSION   DASUTA010000081 DASUTA010000000
VERSION     DASUTA010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157234"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..289)
                     /locus_tag="VFZ80_01575"
     CDS             complement(197..289)
                     /locus_tag="VFZ80_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aurora kinase A-interacting protein"
                     /protein_id="HEX6286150.1"
                     /translation="MGSLVKKRRKKMSKHKYRKRLKANRHKRKK"
     gene            complement(460..1743)
                     /locus_tag="VFZ80_01580"
     CDS             complement(460..1743)
                     /locus_tag="VFZ80_01580"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HEX6286151.1"
                     /translation="MFRGIKESLFTIPVLIVLGCMAAAFGALYLDSTFADSLENWPVV
                     LGTTVAGGRAVLTTIAGATITVAAIVFSITALSTQMAASQYSPRALGGFFEDTFQQAV
                     IGLIVGTFLYALLVLASLSSAIIDGGATATPSVSVTVALILGVVSAIGIVWYINRSLR
                     RMQVDSVVRRIAVASLKAIERNFDAAGEGGAVAASGTPTPDGPPSVVRSSAGGWVVRI
                     DAERALSAIPPSSLARIDVRVGEAVSKDDRIATIWPDPGEDWDGASQVRRSIVTASER
                     SLELDPSFGIRQLVDIALRALSPGVNDPTTAVDVIFHLKTPLRSVLLADAPRRVFVGR
                     EGRRVFLADTPSRSEYVHDAFSEIRLAASGQTHVLSALIDVLEDLKSDLEGESLSGRR
                     SAVEEELELTIEAARQSRFPEQDLRRVLKGHESQG"
     gene            complement(1803..>2764)
                     /locus_tag="VFZ80_01585"
     CDS             complement(1803..>2764)
                     /locus_tag="VFZ80_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015938900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286152.1"
                     /translation="PETGESCVSVIDWLLDSDPAIRWQVMRDLMREPSEVVDTERARI
                     ASEGWGSELLDLQGADGRWDGGTYRPGWVDESKPFFDAWTATHFSLQTLRELGLDPSD
                     PRARRAVSLVRENVRWGANDAPYFAGETEPCINGIALAIGAYFGEEVEGIVDRLMTEA
                     LEDGGWNCRAEYGAKVSSFHTTICVLEGLAAWELAGGSSAEVAEARRRGEEYLLERRL
                     FRSRSTGRVIDPRFTMLSYPTRWYYDVLRGLDHLRSTRATLDERCAEAIELVADKRDR
                     EGRWPLENRHQGPTHFRMGGSEGSPSRWITLRAMRVLDWAGAG"
BASE COUNT          545 a          885 c          859 g          475 t
ORIGIN      
        1 tcgagaacga ggcagccgag gaaacgggcg accaggccga ccgtctcgtc gaagagatcg
       61 aggacttcct caaggacaac tgatcgggtc agtcactggc ttctgggcgg atttctgtcc
      121 ggattccggc gatttctacg cccagaagca ggcccagttg gcgaaaaccg ggcccaagag
      181 cccggttctc ccttgcttac ttcttgcgct tgtgccggtt ggccttgaga cgcttgcggt
      241 atttgtgctt ggacatcttc ttgcggcgct tcttgacgag cgaacccatg cttaacagac
      301 ctctctggac ggggaagact ctccatatgg tcgcgtaccc gccggcatta gtgcaactcg
      361 gggatcggag tgccgaggcg aaggttggaa gtcatcggaa tcccgagcca ggccgctgac
      421 cgaccagtga cacccacggt gccaagaccc caagtggagt cagccctgcg actcgtggcc
      481 tttgagcacg cgtcgcaggt cctgctcggg gaacctcgac tgacgggcag cctcgatggt
      541 caactcgagc tcctcttcga cggcagagcg tcttcccgag aggctctctc cttcgagatc
      601 cgacttgaga tcctccaaca catcgatcag tgcgctgagg acatgtgtct gtcctgaggc
      661 ggcgagccga atctcggaga aggcatcatg aacgtactcg gatcggctcg gggtatcggc
      721 caggaagacc ctgcggccct ctcgacccac gaatacccga cgtggggcgt cggccaacag
      781 aacactgcgc aggggagtct tgaggtgaaa gatgacatcg acggctgtcg tcggatcatt
      841 gacacctggc gagagggcac gaagggctat gtcgaccagt tggcggatcc caaagctggg
      901 gtcgagctcc agagaccgct cggaagcggt cactatggac cggcgaacct gactcgcccc
      961 atcccagtcc tcgcccggat cgggccagat cgtggctatc cggtcgtctt tggagacagc
     1021 ttcgccgact ctcacatcga tccgcgccag cgatgatggc ggaatcgccg agagggcgcg
     1081 ctctgcgtcg atccgaacca cccaaccgcc cgcggatgaa cgaaccacgg aaggcggccc
     1141 gtcaggtgtg ggggtgccag acgccgcgac ggcccctccc tcaccggctg cgtcgaagtt
     1201 gcgttcgatt gctttgaggg aggcgacggc gatgcgacgc acgaccgaat cgacctgcat
     1261 ccttcgaagc gatcggttga tgtaccagac gatgccgatg gctgacacga ccccgagaat
     1321 cagagccacc gtcaccgaga cgctcggtgt cgccgtcgcg cccccatcga tgatcgcgct
     1381 gctcagagag gccagtacca acagggcata gaggaaagtg ccgacgataa gaccgatcac
     1441 cgcctgttgg aacgtgtcct cgaagaaccc gcctagggcg cgcggggaat actggctggc
     1501 tgccatctgg gtggacaggg cggtgatgga gaagacgatt gcggccaccg tgatggtggc
     1561 gccggcgatg gtggtgagca ccgcgcggcc ccccgcaacg gtcgttccca agacgaccgg
     1621 ccaattctcc agagagtcgg cgaaggtgga gtcgagatag agggcgccaa aagcggccgc
     1681 catgcagccg agaacgatca gaaccgggat cgtgaaaaga ctctccttga tccctctgaa
     1741 catggttctc atgtgagatt cgagatttca gacaccaccc aatcaaacca cgcccaagtc
     1801 cattagccgg cacccgccca atcgagcaca cgcatcgcac gtagcgtgat ccaacggctg
     1861 ggtgatccct cggatccgcc catccggaag tgtgtcggcc cctgatgccg gttctccagg
     1921 ggccaccgac cctcgcggtc tcgcttgtcg gccaccagtt cgattgcctc agcacacctc
     1981 tcgtcgagcg tggctcgcgt cgacctgagg tggtcgaggc cacgcaagac gtcgtagtac
     2041 cagcgggttg ggtacgagag catcgtgaat ctcgggtcga tgacgcggcc ggtcgatcgg
     2101 gagcggaaca gtctccgctc cagcagatac tcctctcccc ggcgacgggc ctcggccacc
     2161 tccgcggagg aaccacctgc caactcccat gcggcgagac cttcgagcac acagatcgtc
     2221 gtgtggaagg acgacacctt ggcgccgtac tcggcccggc agttccagcc tccgtcctcg
     2281 agcgcctcgg tcatcagccg atcgacgatg ccttcgactt cctcgccgaa gtaggcacct
     2341 atggccagag ctatgccatt gatgcacggc tcggtctccc cggcgaagta gggagcgtca
     2401 ttggcgcccc agcgcacgtt ctcgcgaacg agagagacag ctcgacgggc acggggatca
     2461 ctcgggtcga ggccgagctc gcgaagggtt tgcagcgaga aatgagtggc cgtccaggcg
     2521 tcgaagaagg gcttcgactc gtcgacccac ccggggcgat aggtcccacc atcccatctg
     2581 ccgtcggcac cctggaggtc gagcagttcg gacccccagc cttcagacgc gatccgggct
     2641 cgctcggtgt cgaccacttc cgagggctct cgcatcagat cgcgcatcac ctgccagcgg
     2701 atggcaggat cggagtccag caaccagtcg atcacgctca cacacgactc tcctgtctcg
     2761 ggcg
//
LOCUS       DASUTA010000082         2053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1711925, whole genome shotgun sequence.
ACCESSION   DASUTA010000082 DASUTA010000000
VERSION     DASUTA010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711925"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2053)
                     /locus_tag="VFZ80_01590"
     CDS             complement(<1..>2053)
                     /locus_tag="VFZ80_01590"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856769.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX6286153.1"
                     /translation="VRVTADFIQAVVDDADWDLIERTSGKVRKTIRARDLWKTIADAA
                     WACADPGLQFDTTINEWHTSPAGGRINASNPCSEYMFLDDTACNLASINLVQLYDDAS
                     GTFDVEGYQHAIRLWTIVLEISVAMAHFPSKEIAQGSYDYRTLGLGYANLGSLLMRQG
                     IAYDSEEGRAIAGAITSVLTGYSYATSAEIAEVIGPFPKYAENEKAMLRVIRNHRVAA
                     YDGDDYDKISTTVMGIDAEVCPSDLLEAARSSWDLALERGEKHGFRNAQASVLAPTGT
                     IGLLMDCDTTGVEPDFALVKFKKLAGGGYFKIVNQSVAPALSHLGYHLDQINEIVTYI
                     VGTSSIEGAPHINTDSLLEKGFTEEDILKLQATLPAVFELKHAFNVFTFGEESLQRLG
                     FTVDDYTSWDFDLLRALGFTRAQIAEANDHICGRQTIEGAPHVKEEHLAVFDTANKNG
                     KYGQRFIHHNGHIKMMAAAQPFISGAISKTINMPHEVTVEEIEEAYHLSGELGLKAMA
                     LYRDGSKAAQPLNSTADEGEENEDEDAAITAAVEAEKAIHWGNLPAGISPTQAYAQGM
                     KPPRFLLPARRSGFNQEARVGGHKVFLRTGEYDDGTLGEIFIDLAKEGATLKGILSCF
                     AIAVSKGLQYGVPLEEFVDTFTFQTFEPRGMVEGHPNIKMANSIVDYVFRSLGVEYLH
                     RD"
BASE COUNT          346 a          619 c          661 g          427 t
ORIGIN      
        1 gtccctgtgc aggtactcga cgccgagcga ccggaacacg tagtccacga tcgagttggc
       61 catcttgatg ttgggatggc cttcgaccat gcctctcggc tcaaaggtct ggaacgtgaa
      121 cgtgtccacg aactcctcga gtggaactcc gtactggaga cccttcgaca cggcgatggc
      181 gaagcagctc aggatgccct tgagtgtcgc tccctccttg gccaggtcga tgaagatctc
      241 gccgagagtg ccgtcgtcgt actcgccggt gcggaggaac accttgtgac ctccgacgcg
      301 ggcctcttgg ttgaacccgg agcgacgggc cgggagcagg aagcgtggtg gcttcatgcc
      361 ttgggcgtac gcctgggtgg gggagatccc ggccggcagg ttgccccagt ggatggcctt
      421 ctcggcttca accgcagccg tgatcgcggc gtcctcatcc tcgttctcct cgccttcgtc
      481 ggcggtggag ttgaggggct gggccgcctt cgaaccgtcc cgatagaggg ccatggcctt
      541 gagcccgagc tcgccggaga ggtgatatgc ctcctcgatc tcctcgacgg tcacctcgtg
      601 gggcatgttg atggtcttcg agatagcgcc ggagatgaac ggctgagccg ccgccatcat
      661 cttgatgtga ccgttgtggt gaatgaacct ctggccgtac ttgccgttct tgttggcagt
      721 gtcgaacacg gccaggtgct cctctttcac atgcggggcc ccctcgatcg tctgtctgcc
      781 acagatgtgg tcgttggcct cggcgatctg tgctctggtg aacccgaggg cgcggagcag
      841 gtcgaagtcc cagctcgtgt agtcgtcgac cgtgaatccg agccgctgca acgactcctc
      901 cccgaaggtg aacacgttga acgcgtgctt cagctcgaac accgccggta gcgtcgcctg
      961 aagcttgagg atgtcctctt cggtgaatcc cttctccagg agcgagtcgg tgttgatgtg
     1021 cggggcgccc tcgatcgagc tcgtccccac tatgtacgtg acgatctcat tgatctgatc
     1081 gaggtggtat cccaggtggg aaagcgccgg ggcgaccgac tggttgacga tcttgaagta
     1141 gccccctcca gccagcttct tgaatttgac cagggcgaag tcgggctcga caccggtggt
     1201 gtcacaatcc atcaacaacc caatggtgcc ggtcggcgcc aagacactgg cttgtgcgtt
     1261 gcggaatccg tgcttctccc cacgctccaa agccaggtcc cacgacgatc tagccgcttc
     1321 gagaaggtcg gacgggcaga cctcggcgtc gatgcccatc acagtcgtgg agatcttgtc
     1381 gtagtcgtcg ccgtcgtacg cagccacccg gtggttgcgg atcacccgca gcatcgcctt
     1441 ctcgttctcg gcgtacttcg ggaatggtcc gatgacctcg gcgatctcgg cggaagtcgc
     1501 gtatgagtag ccggtgagga ccgatgtgat ggcgccggcg atggcgcggc cttcctcgga
     1561 gtcataggcg atgccctgac gcatcagcaa cgaccccagg ttggcgtagc cgagaccgag
     1621 agtccgatag tcgtaggacc cttgggcaat ctccttcgaa gggaagtggg ccatggccac
     1681 ggatatctcg aggacgatgg tccagagcct gatggcgtgc tgatatccct cgacgtcgaa
     1741 cgtgcctgag gcgtcgtcgt agagctgcac cagattgatc gacgccagat tgcacgccgt
     1801 gtcgtcgaga aacatgtact cgctgcaagg gtttgatgcg ttgatccgtc ctccagccgg
     1861 gctggtgtgc cactcgttga tggtggtgtc gaactggaga ccgggatcgg cgcacgccca
     1921 ggcggcgtcg gcgatggtct tccacaggtc acgggctcgg atcgtcttgc gcaccttgcc
     1981 gctggtccgc tcgatcaggt cccagtcggc atcgtcgacg acggcctgga tgaagtcggc
     2041 cgtgacacgc acc
//
LOCUS       DASUTA010000083         7851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_288301, whole genome shotgun sequence.
ACCESSION   DASUTA010000083 DASUTA010000000
VERSION     DASUTA010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7851
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288301"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..884
                     /locus_tag="VFZ80_01595"
     CDS             96..884
                     /locus_tag="VFZ80_01595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX6286154.1"
                     /translation="MARAIWKGAINFGLVTIPVGMYTATDDLTPKFKQLRKTDHSAIR
                     YKRVAEADGEEVQWDDIVKGYEFEKDRYVVFTDEELSAANPEGDKLVDVIQFVDEDEI
                     DPMMYQKAYYLAPEKTGIKAYRILKEALEDKGRVGIAKVSIREKQQLATIRVKDDVIV
                     METMFWPDEIRAAEFEELGANVEIRPEEVEMAGSIIDNLTRPFDGEAYVDKTREAVEQ
                     LAEKKVAGEEIVAPASPEPTKVVDLLDALKASVEATKQKQAKAG"
     gene            925..1785
                     /locus_tag="VFZ80_01600"
     CDS             925..1785
                     /locus_tag="VFZ80_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX6286155.1"
                     /translation="MARYGAVEIGGTKTDAAVGSSPDTFSTPHRIATTTPEETLGEVA
                     KFLVSHDVAAIGVASFGPLDLDASSPGYGSILATPKAGWNGASVLEAIKSAAGVPVGI
                     DTDVNGAALGEGRWGAARGMSDYVYVTVGTGIGAGVVVNGRPISGERHPETGHLVVSR
                     HERDDYPGNCPYHGDCLEGMASGPALEARFGRSDTWAGNEAVLDLVTHYVGQGLRDLV
                     YTVAPERIIVGGGVSRLPFFHERIRIVLSGLLAGYPADPDLELLVSKPGLGQLSGLAG
                     GLVLAERASS"
     gene            1785..2984
                     /locus_tag="VFZ80_01605"
     CDS             1785..2984
                     /locus_tag="VFZ80_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HEX6286156.1"
                     /translation="MGTPTLHIRPGNPDFLDLPWDRRLDDWDHERLVTMPVGIHRHPV
                     LFVAYEEGIYAIKELGRRTARGEYSVLRTLEDQTQRTARPAGLVERDWLNAHDEGAGA
                     VITRYVEHAFPYRRLVSGAGFGSRRRHMADALAGLFVELHMAGCYWGDCSLSNVLYRF
                     DAGQIEAIMIDAETSELHPELSTGQRRQDIEIMMENVAGEMADVAAETGEDIDSADIE
                     LGVEIANRYEVLWAELTEELVITRNDVYRIRERISRINELGFAVEDIDITPTGDGSLV
                     KMITNLGGRTYNSDMLHERTGIEASENQACLILSDLSYHLAKHGDVTATGKTVGTFKW
                     LTDYYEPLIARIAADWPGEDPIQGYCDYLNHRVRLAHARGTDVDSFEAYEDWVESGFP
                     GFDPEET"
     gene            complement(2985..3773)
                     /locus_tag="VFZ80_01610"
     CDS             complement(2985..3773)
                     /locus_tag="VFZ80_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6286157.1"
                     /translation="MDRVAVVTGANHGIGAAIAVALARSGVRVLATYLRMEAVSEPST
                     YEMNRMSSTEDLVRQVESGEGVAVGLEVDLTSEGAAALIFDRAEQRLGPVSILINNAS
                     AWTADTFADAEEDRLGRPLSRLSEATFDSVFAVDARASALLIAEFAVRHQRHDLDWGR
                     IVGLTSGDPGGFPEEVSYGAAKAAMENLTMSAAFELADRGITANVVHPPVTDTGWVDD
                     EVRRVVAASPKMFHVAAPDEVAAVVAFLCSDAADLITANRIHLR"
     gene            complement(3802..5034)
                     /gene="ugpC"
                     /locus_tag="VFZ80_01615"
     CDS             complement(3802..5034)
                     /gene="ugpC"
                     /locus_tag="VFZ80_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX6286158.1"
                     /translation="MAAITMENLGKIYEGGTRAVGGVDLEIHDGEFLVLVGPSGCGKS
                     TVLRMIAGLEEISEGVLKIGDRVVNDVPPKERDIAMVFQNYALYPHMSVYDNMAFGLK
                     LRKMDKKEIERRVHEAAEILEITEFLDRRPKALSGGQRQRVAMGRAIVREPAAYLMDE
                     PLSNLDAKLRVQMRSELERLHARLGTTTVYVTHDQVEAMTLGDRVAVLRAVSPRAPYN
                     LQQVGPPTELFDSPDNLFVAGFIGSPAMNLTYGHLEAGEGDDVWAVLPQTRLLVSPSS
                     LQKHPGIENWTGKEIVVGLRPNNLEAASVAGHDPDRTLEAEVEVTEMLGADTYIHFTV
                     NRSPVVTPDIEELLADSGQDASSLGETTNFIARVSPDVNVKHGETVQLTVDCDKLLFF
                     DPDTGMRIGVKEPAAATA"
     gene            5281..5610
                     /locus_tag="VFZ80_01620"
     CDS             5281..5610
                     /locus_tag="VFZ80_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286159.1"
                     /translation="MWSAPTFQHCPFDDSPDPSQCGIPVPWGEFDNTAWLSGIWEGRL
                     IQPDVFVYDSHLRQSVVGSAPNGAEVEIVLYQENPVLDYYMVRIQGDSQIEGWLPEPF
                     LSFDPVT"
     gene            5637..6170
                     /locus_tag="VFZ80_01625"
     CDS             5637..6170
                     /locus_tag="VFZ80_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286160.1"
                     /translation="MSEDKQERSNLRRRWLAVLLATVLMIGSFVLFVYSLAAASGNEN
                     AFAGGLLGIGLGLVPAVFAAAAWASQNPNTVRSVVFATLLFFAAFLLIGYFSLPVALV
                     AGYGAGGVVAFRLGPENSRRTRAIAVALSVIYTGALLTFSTALGLFAGAALPFLSIAL
                     ADLYEERGTGPSEEVSP"
     gene            complement(6160..7098)
                     /locus_tag="VFZ80_01630"
     CDS             complement(6160..7098)
                     /locus_tag="VFZ80_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6286161.1"
                     /translation="MTDMTATDLDLTGEREGLTELDHARARRRKRFWSRFGFWVLIVL
                     IAIFLLFPFYWAINSSLKTEPQLRMTPATFVPRDPGTGEVSITLQNYEAAFQNDAFLR
                     AIGNSAIVAVSVTVIALVIGSFAGFALGKLRFRGKTPSLYLILSMTMFPQVAVLTGLY
                     AVILNFDIPAIPSMIISYMLFTLPFTVWVLTAFFQQLPIDLLQSAQVDGATPFQSFRM
                     ILLPLTMPALVTTGLLAFIGAVNEYLFALTFTTIEPNARTIPVSIALFTGEIARQEPF
                     GEIMAAAVVVTIPLIVLVLIFQNRIIGGLTAGAVKG"
     gene            complement(7091..>7851)
                     /locus_tag="VFZ80_01635"
     CDS             complement(7091..>7851)
                     /locus_tag="VFZ80_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6286162.1"
                     /translation="EPRRYREVFTFGLFGNQYVMGATDPAFILSIADTLIFTVATIAL
                     EVVIGLGIALVLNAPFRGRGGMRAAMLVPWAILTAVSSRIWEWMFIDTRAGFFNVVFE
                     RVGFIDQPVPWIASETWQLPVAITIDVWKTTPFMALLLLAGLALIPDELYEAAEVDGA
                     SKWNQFRSITLPLLKPTLAVALVFRTLDALRVFDLFQIVFAQSKYSMASFAYYELIDS
                     QRLGYSSAASVVIFFVITIFAILYIKSLGIASDD"
BASE COUNT         1533 a         2429 c         2444 g         1445 t
ORIGIN      
        1 cacgacgaga gtgcaggagg ttgcggtttt cgccacgcca tgcactctcg acgtccccaa
       61 cgcccgtgtc ccgagaccgg cgtagaatca cacgcatggc tagagccatt tggaagggcg
      121 cgatcaactt cggcctggtg accatcccgg tggggatgta caccgccacc gacgacctga
      181 cgcccaaatt caaacaactt cgcaagaccg accattccgc catccgctac aagcgagtcg
      241 ccgaagccga cggcgaggaa gtccagtggg acgacatcgt caagggttac gagttcgaga
      301 aagatcgcta cgtcgtattc accgacgagg agttgtccgc cgccaacccc gagggcgaca
      361 agctcgtcga cgtcatccag ttcgttgacg aggacgagat cgacccgatg atgtatcaga
      421 aggcctacta cctcgctccc gagaagaccg ggatcaaggc ttaccgcatc ctaaaggagg
      481 cgctcgagga caagggccgg gtcggcatcg ccaaggtctc gatccgcgag aagcagcagc
      541 tcgccaccat ccgcgtcaaa gacgacgtca ttgtcatgga gaccatgttc tggccggacg
      601 agatacgagc cgccgagttc gaagagcttg gcgccaatgt ggagatcagg cccgaggagg
      661 tcgagatggc cggctccatc atcgacaatc tgacccgtcc tttcgatggc gaggcgtacg
      721 tcgacaagac ccgggaggct gtcgagcaac tcgccgagaa gaaggtcgcc ggcgaggaga
      781 tcgttgcgcc ggcctcacca gagccgacca aggtcgtcga cctcctcgac gctctcaagg
      841 cctccgtcga agccaccaag cagaagcagg caaaagcggg ctgacagtcg ccgagtcgga
      901 ggcactcact tagagtccgg tcccgtggca cgatacggcg cggtcgagat cggcggcacc
      961 aagacagatg ccgcggttgg atcgtccccg gacacgtttt cgacgccaca tcggattgcc
     1021 accaccacac ccgaggagac cttgggtgaa gtagccaagt tcctcgtctc gcacgatgtc
     1081 gcagcgatcg gcgtagccag tttcggcccg ctcgatctcg acgcgagcag ccccggatac
     1141 ggctcgattc tggccacacc caaggcaggc tggaatggcg cttcggtgct cgaggccatc
     1201 aaatccgcag ccggggtacc tgtgggcatc gacaccgacg tcaacggggc tgccctcggt
     1261 gagggacgat ggggtgccgc tcgaggtatg tcggactatg tctatgtcac ggttggcacc
     1321 ggcatcggtg cgggggtggt ggtcaacggt cgacccatca gcggcgagcg ccaccccgag
     1381 accggccatc tcgtggtgag tcgacatgag cgtgacgact atccgggcaa ttgcccctat
     1441 cacggcgatt gtctggaagg gatggcaagc ggaccagcgt tggaggcacg attcgggcgt
     1501 tccgacactt gggccggcaa cgaggccgtg ctcgacctcg tgacccacta tgtgggccag
     1561 gggttgcgcg acctcgtcta taccgtcgcc cccgagcgga tcatcgtcgg tggcggagtc
     1621 tcccgcttgc cgttctttca cgagaggatt cgcatcgtgt tgagcgggct cctagccggc
     1681 tatccagccg atcctgacct cgagctcctc gtctcgaaac cgggcctcgg tcagctgtcc
     1741 gggttggccg gaggtttggt cctggcggag cgcgcaagca gctgatggga acgcccacac
     1801 ttcacattcg cccgggcaac cccgacttcc tcgaccttcc gtgggaccgt cgactcgacg
     1861 attgggatca cgagcggctg gtcactatgc cggtcgggat ccaccgtcat ccggtgctct
     1921 tcgtcgcgta cgaggagggt atctatgcca tcaaggagct cggtcggcgc accgcccgcg
     1981 gggagtactc ggtccttcgc actctcgagg accagacaca aaggacggcc cggcccgctg
     2041 gtctcgtgga gagagactgg ctcaacgcgc acgacgaggg tgcgggtgcc gtgatcacgc
     2101 gttacgtcga gcacgctttc ccctaccgtc gcctggtttc gggagccgga ttcgggtctc
     2161 ggagacgtca catggccgat gccctcgccg ggctgttcgt ggagctccac atggcggggt
     2221 gctattgggg cgactgctcc ttatcgaacg tgctgtaccg gttcgatgcc gggcaaatcg
     2281 aggcgatcat gatcgacgcc gagacctccg agctacatcc cgagctcagc accggtcagc
     2341 gacgccagga catcgagata atgatggaga acgtcgctgg tgagatggca gacgttgccg
     2401 ccgagaccgg cgaggacatc gactcggccg atatcgagct cggagtggaa attgccaacc
     2461 gttacgaggt cttgtgggcg gagttgaccg aagagctggt catcacacga aatgacgtct
     2521 accgaatcag ggagcggatc tcacgcatca atgagctggg cttcgccgtc gaggacatcg
     2581 atatcactcc gaccggcgat ggaagcctcg tcaagatgat caccaacctc gggggtcgca
     2641 cgtacaactc ggacatgctt cacgagagga ccgggatcga ggcgtccgag aatcaggcgt
     2701 gcctcatcct cagcgacctg agttatcacc tggcgaaaca tggggatgtc acagccaccg
     2761 gcaagaccgt tgggaccttc aaatggctca ctgactacta cgagccgctc attgcccgca
     2821 tcgcggcgga ctggcccggc gaagacccga tccagggata ttgcgactac ctgaaccatc
     2881 gcgtgcgtct cgcccacgct cggggcaccg acgtcgactc cttcgaggcc tacgaggatt
     2941 gggtggagtc agggttcccc ggattcgacc cagaagagac gtgactatcg aaggtggatt
     3001 cgattagccg ttatcaggtc tgcagcatcc gagcagagga aggcgaccac ggcagcaact
     3061 tcgtccggtg ccgcgacatg aaacatcttg gggctcgccg ccaccacccg acggacctca
     3121 tcgtcgaccc agccggtgtc ggtcactggt ggatggacca cgttggctgt tatcccgcga
     3181 tcggcgagct cgaacgccgc ggacatcgtg agattctcca tggcggcttt cgccgcccca
     3241 taagagacct cctcggggaa cccgccggga tcgcctgacg tcagcccgac aatgcgtccc
     3301 caatccaagt cgtggcgctg gtgtctgacg gcaaactccg cgatcagcag ggccgatgcc
     3361 cgcgcatcga cggcgaagac cgaatcgaac gtggcttcgc tcagcctgga gagcggtcgg
     3421 ccgagacggt cttcctccgc atcggcgaac gtgtcggccg tccaggcact cgcattgttg
     3481 atgagaatgc tcaccgggcc caggcgctgc tcagctcgat cgaatatgag ggcggctgcc
     3541 ccttcggatg tgaggtcgac ttccaagccg acggcaactc cttcaccgga ttccacctgc
     3601 ctgaccagat cctcggtcga agacatccgg ttcatctcgt acgtggaggg ctccgagaca
     3661 gcctccatcc gcagataggt ggcaagcacc ctcacgccgg atcgagccaa ggcgacggcg
     3721 atagccgctc ctatcccgtg gttggctcct gtgacgaccg cgacgcgatc catcaggccg
     3781 ccgagttgtc cgctgaagcc gtcaggcggt ggccgccgcc ggctctttca ccccgatgcg
     3841 catcccggta tccggatcga agaacaagag cttgtcgcaa tcgaccgtca attgcaccgt
     3901 ctcgccgtgc ttcacgttca catcggggct gacccgtgcg atgaagttgg tggtctcacc
     3961 gagcgaggaa gcgtcctgac cggagtcagc gagcaactcc tcgatgtcgg gcgtgaccac
     4021 agggctccgg ttcacggtga agtggatgta ggtgtcggcg ccgagcatct cggtgacctc
     4081 gacctcagcc tccagggtcc gatcggggtc gtggccggcc accgatgccg cctccaggtt
     4141 gttggggcga aggcccacca ctatctcttt gccggtccag ttctctatgc cgggatgttt
     4201 ctgaagggag gaggggctga ccagcagccg ggtctggggc agcacggccc agacatcgtc
     4261 accctcaccc gcttccagat ggccataagt caggttcatg gccgggctcc cgatgaaccc
     4321 ggcgacgaag agattgtcag ggctgtcgaa caactcggtg ggcggaccga cctgctgcag
     4381 gttgtagggc gctcgcggag agacggcccg caggaccgcc actcgatctc cgagcgtcat
     4441 cgcctccacc tgatcgtggg tcacgtagac ggtcgtggtt ccgagacgtg cgtgaagtct
     4501 ctcgagctcc gaacgcatct gaacgcgcaa cttggcatcg agattggata ggggctcgtc
     4561 catgagatat gccgctggtt cgcggacgat tgccctcccc atcgctacgc gctgacgctg
     4621 tcccccggac agggccttgg gccgacgatc gaggaactcc gtgatctcga ggatttcagc
     4681 tgcctcgtgt acccgacgct cgatttcctt cttgtccatc ttccgcagct tgagcccaaa
     4741 cgccatgttg tcgtagacgc tcatgtgcgg gtacagggcg tagttctgga agaccatggc
     4801 tatgtccctt tccttgggtg ggacatcgtt gaccactcga tcgccgatct tcaagacacc
     4861 ttcggaaatc tcctcgaggc ccgcaatcat ccggagaacg gtcgacttgc cacagccgga
     4921 gggccccacc aggacgagga actcaccgtc atggatctcg aggtcgacgc ctccaactgc
     4981 gcgcgtgccg ccttcgtaga tcttgccgag gttctccatc gtgatggcag ccatctcatt
     5041 actcccagta ggcaggaatt ttcacaaccg aacctagccg atgcgatcgc atgttgtcga
     5101 cgcgatgagc ggcgcggact agctgcctac acacccgtgt tctaccggtc atgaggaggg
     5161 tcctgctcgc tgtggccatg gccgtcgcat tcggagcctc catatggctc ggcgccgtag
     5221 tgttcgaccg tggggaccgg caggcgtccg gcgaaaccat cgagacccac ctcgtctccg
     5281 atgtggagcg cgccgacgtt ccaacattgc cctttcgacg acagcccgga tccgtcacaa
     5341 tgcgggatcc ctgtgccctg gggtgagttc gacaacaccg cctggctctc cggaatctgg
     5401 gaaggtcggc tcatacagcc cgatgtcttc gtgtacgaca gccatctacg gcagagcgtg
     5461 gtcggaagcg ctccgaacgg cgcggaggtg gagatcgtcc tctatcaaga gaacccggtc
     5521 ctcgattact acatggttcg catccaaggt gactcccaga tcgaaggctg gctcccggag
     5581 ccattcctct cgttcgatcc cgtcacttag ggggcgtcgc cctacgctct gcaagagtgt
     5641 ctgaggacaa gcaggagcga tccaatctcc gtcgtcgctg gctggcggtt ctgttggcca
     5701 ccgtcttgat gatcggttcg ttcgttctct tcgtgtattc gctcgcagcc gcctcgggga
     5761 acgagaacgc ctttgccggc gggctgctgg gaatcggcct cgggttggtc cccgccgtct
     5821 tcgccgccgc cgcctgggcc tcgcagaacc cgaacaccgt caggtccgtc gtattcgcca
     5881 ccctgctctt cttcgccgct ttcctcttga tcggctactt cagtcttcct gtggctctag
     5941 tcgccggtta tggggcaggt ggggtggtcg ccttcaggct gggtccggag aattcacggc
     6001 gcacgcgagc catcgctgtc gccttgtccg tcatctacac cggcgcgctg ttgaccttct
     6061 caaccgcact ggggctgttt gctggtgccg cattgccctt cctctcgatc gctcttgcgg
     6121 acttgtacga agagcgcggc acggggccgt ctgaagaggt cagcccttga cggcgcccgc
     6181 ggtgagaccg ccgatgatcc tattctggaa gatcaggacg agcactatca gtggaatggt
     6241 gaccacgacg gcagcggcca taatctcgcc gaacggttcc tgacgggcga tttcaccggt
     6301 gaagagggct atagacaccg ggatcgtcct cgcattcggc tcgatggtgg tgaatgtcag
     6361 tgcaaacagg tactcgttca cggctccgat gaaagccaag agaccggtgg tgaccagtgc
     6421 aggcatcgtc aacgggagaa ggatcattcg gaacgactga aaaggcgtcg ccccgtccac
     6481 ctgggcggac tgcagtaggt cgatcggcaa ctgctggaag aaggcagtca gcacccagac
     6541 cgtgaacggc agagtgaaca gcatgtagct gatgatcatc gatgggattg ccggtatgtc
     6601 gaagttcagg atcacggcgt agagaccggt cagcaccgct acctgaggga acatcgtcat
     6661 cgacaggatc agatagagcg acggtgtctt cccccggaac cgcaacttgc ccagggcaaa
     6721 cccggcgaac gatccgatga cgagagcgat cacggtcacc gagaccgcca cgatggcgct
     6781 gttgccgatc gcccggagga acgcgtcgtt ctggaaggcc gcctcatagt tctggagggt
     6841 gatcgagacc tcgccagtgc cggggtcacg cgggacgaag gtcgccgggg tcatccgcag
     6901 ttgcggctcg gtcttgagcg acgagttgat cgcccagtag aaggggaaaa ggaggaatat
     6961 ggcgatgagg acgatgagta cccagaatcc gaacctcgac cagaacctct ttcggcggcg
     7021 agctcgagcg tggtcgagct cggtgagacc ttcccgttcc ccggtgagat cgaggtcggt
     7081 ggctgtcatg tcagtcatct gatgcgatcc ccagactctt gatgtacaag atggcgaaga
     7141 tcgtgatcac gaagaagatc accaccgacg ccgcggagga ataaccgagc ctctgactgt
     7201 cgatcagctc gtagtaggca aagctggcca tcgaatactt gctctgggcg aagacgatct
     7261 ggaacaagtc gaagacacga agcgcgtcga gtgtcctgaa caccagggcc acggcgaggg
     7321 tcggcttgag gagcggaagt gtgatggaac ggaattggtt ccacttgctg gcgccgtcga
     7381 cctcggcggc ttcatatagc tcgtccggga tgagagccag cccggccagc aacaacagcg
     7441 ccatgaacgg tgtcgtcttc cagacgtcga tcgtgatcgc caccggaagc tgccaggtct
     7501 cgctggcgat ccagggtacg ggctggtcta tgaagcccac tctctcgaac acgacgttga
     7561 agaacccagc tcgcgtgtcg atgaacatcc actcccagat ccgggaggac accgccgtga
     7621 ggatcgccca ggggaccagc atggccgccc gcatcccacc cctcccccgg aaaggtgcgt
     7681 tgagcaccag agcgatgccc aagccgatga cgacctccag ggcgatcgtg gccactgtga
     7741 agatgagggt gtcggcgatg ctgaggatga aggccggatc cgtggcaccc atgacgtact
     7801 ggttgccaaa caacccgaat gtgaacacct cccggtaacg acggggctca c
//
LOCUS       DASUTA010000084         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1325238, whole genome shotgun sequence.
ACCESSION   DASUTA010000084 DASUTA010000000
VERSION     DASUTA010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325238"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VFZ80_01640"
     CDS             complement(<1..648)
                     /locus_tag="VFZ80_01640"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX6286163.1"
                     /translation="MSRRRSATTTSDFGSGKRESHDSTSFYARFTPPAVSSDDRVSSP
                     AVVDELFCGDARMMGDTQVADRSVALLVTSPPYFAGKEYETDMAAGHVPATYIEYLEM
                     LHDVLAVSLAKLEPGGRMAINVANLGRKPYRSLSADIISILQDDLGMLLRGEVIWQKA
                     KGAGGSCAWGSYRSPQNPVLRDVTERIVIASKGRFDRAVKRPVRERDGLPHEATID"
     gene            720..1313
                     /locus_tag="VFZ80_01645"
     CDS             720..1313
                     /locus_tag="VFZ80_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6286164.1"
                     /translation="MKIVIPADRPKYERSLPSGDDLTLSPILKTDREFFERGIEELSL
                     ESRFARFGQGVGRLSDQELDYLSDVNQRSHVAWGALVGDEAAGVGRYIVTDDPSRAEI
                     ALTVLDQMQRKGVGRALFEALIAVARHDGIHEFTFEAQSDNEAVVNLMREIEVTPLIA
                     DGVIERSIRLSDLPASPQDHALVDVIEEVRSQRDAST"
     gene            complement(1310..1708)
                     /gene="msrB"
                     /locus_tag="VFZ80_01650"
     CDS             complement(1310..1708)
                     /gene="msrB"
                     /locus_tag="VFZ80_01650"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX6286165.1"
                     /translation="MTDMPKTESEWREQLSPEQYQVLRQGGTERPFTGKYWDSKAEGV
                     YRCAGCGEKLFSSDTKYESHSGWPSFFQPLANDLIDEEVDRSFGMTRTEIKCANCGGH
                     LGHVFDDGPNPSGLRYCVNSASLDLEEAGS"
     gene            complement(1705..2178)
                     /locus_tag="VFZ80_01655"
     CDS             complement(1705..2178)
                     /locus_tag="VFZ80_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013005282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX6286166.1"
                     /translation="MSTPMRSAGLLPYRFEGALEVLIAHPGGPWFANKDAGSWSVIKG
                     IVKDGEDDEDAAAREFSEETGWDPPTDEAWVPLGETTLRSRKVVVAWAVETDFDISEF
                     APGTFTLYGKEYPEIDEVVWMAPTEARHKLNPAQTVFIDRLERHLGLNGSEEETG"
BASE COUNT          404 a          671 c          692 g          411 t
ORIGIN      
        1 gtcgatcgtg gcttcgtggg gaagcccgtc tcgttcacgg acgggacgct tgacggcgcg
       61 atcgaagcgg cccttggaag cgatcacgat cctctcggtg acatcacgga gaaccgggtt
      121 ctgcggcgag cgataagagc cccaggcaca gctcccacct gcccctttgg ccttctgcca
      181 gatcacctca ccccgcagca acatgcccag gtcgtcctgg aggatcgaga tgatgtccgc
      241 ggatagtgag cggtagggct tgcgacccag gttggccacg ttgatcgcca tccgtccccc
      301 gggctcgagc ttggcaagtg agacggcgag gacgtcgtgc agcatctcca ggtactcgat
      361 atacgtggcc gggacatggc cggcggccat gtcggtctcg tattccttgc cggcgaaata
      421 cggtggcgag gtgaccagta gggcaactga gcggtctgcg acctgagtgt cgcccatcat
      481 tcgcgcgtcc ccgcagaaga gctcgtcgac taccgctgga gaggagacgc gatcgtccga
      541 tgacaccgca ggcggggtga atctggcata gaacgaggtc gagtcgtgcg actcccgctt
      601 ccctgagccg aagtctgagg ttgtcgtggc cgatcgccgc ctggacatgg ctcttgaagt
      661 tatccatcgt cgacgtgaac tgactgacaa tcgggaaacg gcttcaattg cgggatcgca
      721 tgaagatcgt catccctgcc gaccggccga aatacgagag aagcctcccg agcggggacg
      781 atctcacgtt gagcccgata ctcaagaccg accgggagtt cttcgaacga ggcatcgagg
      841 agctgtcact ggagagcagg ttcgcccgat tcggacaggg agtcggcaga ctaagtgatc
      901 aggagctcga ctacttgagc gacgtcaatc agcggagtca tgttgcctgg ggcgccctgg
      961 tcggagacga ggcagccggt gtgggtcggt acatcgtcac cgacgacccg tcgcgcgccg
     1021 agatcgccct caccgttctg gatcagatgc agcgcaaggg ggtgggccgg gctcttttcg
     1081 aggctctgat cgcggtggcc cgccacgacg ggattcacga gttcaccttc gaggcccaat
     1141 cagacaacga agcggtcgtc aacctgatgc gtgagatcga ggtaacgcca ctcatcgccg
     1201 acggtgtgat cgagcgctcg atcagactct cagacctgcc cgccagcccc caagaccacg
     1261 cactcgtcga cgtgatcgag gaggtgaggt ctcagaggga cgcctcgact taggagccgg
     1321 cctcttcgag gtcgagggag gccgagttca cgcagtagcg gaggccgctc gggttgggcc
     1381 cgtcatcgaa gacatgaccg aggtggcccc cgcagttggc gcacttgatc tcggtgcggg
     1441 tcatcccgaa ggagcgatcg acttcctcat cgatcaggtc gttggcgagt ggctgaaaga
     1501 agctgggcca accgctgtgt gactcgtact tggtatcgga tgagaacagt ttctctccgc
     1561 acccggcgca ccggtagacg ccctcagcct tggagtccca gtacttcccg gtgaagggcc
     1621 gttcagtgcc gccctggcgg agaacctgat actgctccgg agacagctgt tcccgccatt
     1681 ccgattcggt ctttggcatg tcagtcatcc tgtttcctcc tcgctcccat tcaaccccag
     1741 gtgccgctct aaacgatcga tgaagacggt ctgggccggg ttgagcttgt gtcgcgcctc
     1801 ggtgggcgcc atccacacga cctcgtcgat ctcagggtat tcctttccgt agagcgtgaa
     1861 cgtgcccggg gcgaactccg agatgtcgaa gtcggtctcc accgcccagg caacgacgac
     1921 tttgcgcgac ctcaacgtcg tctctccgag tggcacccac gcttcgtccg tgggagggtc
     1981 ccacccggtc tcctccgaga actcgcgggc cgcggcgtct tcgtcgtcct caccgtcctt
     2041 gacgatccct ttgatgaccg accatgaccc ggcgtccttg ttggcgaacc agggcccgcc
     2101 gggatgggcg atgaggactt ccaaagcgcc ctcgaaacgg taggggagaa gacccgcgga
     2161 tctcatgggg gtcgacat
//
LOCUS       DASUTA010000085         3229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_289191, whole genome shotgun sequence.
ACCESSION   DASUTA010000085 DASUTA010000000
VERSION     DASUTA010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289191"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VFZ80_01660"
     CDS             complement(<1..520)
                     /locus_tag="VFZ80_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEX6286167.1"
                     /translation="MPISNLHGLAAAGVSIWSDQISRQMLDSGELARRVEQDAVTGVT
                     SNPTIFAKAISASSDYDAQLHELKSGGLTPQEVAKSLMADDIARACDTLATVFTATGG
                     RDGFVSVEVSPTLAANTDATIAEARDWVKQIDRENLFVKVPATPEGVPAIRALIAEGI
                     SVNVTLIFSLQRY"
     gene            complement(609..1469)
                     /locus_tag="VFZ80_01665"
     CDS             complement(609..1469)
                     /locus_tag="VFZ80_01665"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6286168.1"
                     /translation="MDEATQLVDRLLVQAQALSAVVAHLRLLSSDVPGDPAVNAELQR
                     VIDALGAKSALDSLSPGERNVLANLAQSYLAQAIDLATDPTRPGEWTLSDPEILQSQG
                     TASGGVATLIAEAGIGAPGIRILDVGTGVGGLAIAFCEMYPGSTVVGVDPWEPALSLA
                     RENIAEAGLEERVTLIAGRLEDFVDAEGFDLIWVPSFFISEVVLNQALLRVYQATRPG
                     GKVVMGIMGGSEKPLAEAVDRLFTVRSGGSNMSVDEGMAGLRRSGFDDVAEIQKTWQI
                     PLRLVLASRT"
     gene            1561..1974
                     /locus_tag="VFZ80_01670"
     CDS             1561..1974
                     /locus_tag="VFZ80_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX6286169.1"
                     /translation="MPRRILIAKPGLDGHDRGAKVIARALRDAGHEVIYSGLHQTPAQ
                     IAETAVQEDVDAVGLSSLSGAHKTLFPQVIAELADRDSSDIIVFGGGVIPDADKAALE
                     DAGIVMIFTPGTPLDEITGWVDRSIPSRAQQRRGA"
     gene            1971..3194
                     /gene="sucC"
                     /locus_tag="VFZ80_01675"
     CDS             1971..3194
                     /gene="sucC"
                     /locus_tag="VFZ80_01675"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX6286170.1"
                     /translation="MKIHEHQAKQLMAARGIPVPLGRVARSVDEAVAAVRPLIDESGN
                     PVVVLKSQIHAGGRGKGTFREHPDVRGVNVITDGIQGGPSAAEEAVRELAKKMLGSTL
                     VTIQTGDEGKKVNRLYVEQGIDIDRELYLAVLLDRATSRNIVMASTEGGVEIEKVAEE
                     TPEKILRAQIDPAIGLAPFQANELVHGLGIEGDAAKNFTRFVMNLADAAPELDTDLIE
                     INPLVITKTGEVMALDGKMSFDDNALYRHPDIADMRDETEEDPLELAAKNAGLNYIKL
                     DGTIGCLVNGAGLAMATMDTIKHVGGEPANFLDVGGGATADQVTTAFQIITKDPSVKG
                     IFVNIFGGIMRCDVIATGIVEAVERVGLEVPLVVRLEGTNVEEGKDIIEKSGLEVTNA
                     DSLRDGAEKILELST"
BASE COUNT          642 a          984 c         1053 g          550 t
ORIGIN      
        1 cataacgctg cagggagaag atgagcgtaa cgttcactga gattccctcg gctatcaatg
       61 ctcggatggc ggggacacct tccggcgtgg ccgggacttt cacgaacagg ttctcgcgat
      121 cgatctgctt cacccagtca cgtgcctcgg cgatggtggc gtcggtgttc gcggcgaggg
      181 tcggcgacac ctccaccgac acgaaaccat cacgtccccc ggtggcggtg aagaccgtgg
      241 cgagggtgtc acacgctcga gcaatgtcat ccgccatcag actcttggcc acctcctggg
      301 gggtcaggcc acccgatttg agctcgtgga gttgggcgtc gtagtcactc gatgcactga
      361 tcgccttggc aaagatcgtc gggttggagg tcacaccggt gactgcgtct tgttcgacac
      421 ggcgcgccag ctcaccggag tcgagcatct gccgggagat ctgatccgac cagatgctga
      481 ctccggctgc ggccagcccg tggaggttgg agatgggcat gagctaaggg tagaggtgtt
      541 gagtctcact cacgatatcg gacaggctgt tgcctttgaa acgggtagcg cggactcgga
      601 ctcgaccgtc aggtgcggct ggccaggacg aggcgaagag gtatctgcca ggtcttctgg
      661 atctcggcga catcatcgaa acccgagcgc ctcagccctg ccatgccctc atcgacactc
      721 atgttggacc cgcctgatcg gaccgtgaag agccgatcga ctgcttctgc cagaggtttc
      781 tctgacccgc ccatgatccc cattacgacc tttcccccgg ggcgcgtcgc ttggtagaca
      841 cgcaacaacg cctggttcaa gacgacctcg gagatgaaga aggacggaac ccatatcaga
      901 tcgaatccct cggcgtcgac gaagtcctct aggcggccag ctatcagggt gacccgctct
      961 tccaagccgg cctcggcgat gttctcacgg gcaagagaga gcgccggctc ccacgggtcg
     1021 actccgacga ccgtcgatcc cgggtacatc tcgcagaacg caatagcgag cccgccaacc
     1081 ccggttccga catccaggat tcgtatcccc ggggcaccga tgcctgcttc cgcgatcagg
     1141 gtggccacgc cgcccgacgc cgtcccctgc gactgaagga tttcaggatc gctgagggtc
     1201 cactcacccg gtcgggtggg gtcggtcgcc aggtcgatgg cttgggcgag ataagactgg
     1261 gccaggttcg cgagcacgtt tcgctcgcca gggctcagcg agtcgagggc cgatttcgct
     1321 cccagggcgt cgatgacccg ttgcagctcg gcgttgacag cgggatcacc cgggacgtcg
     1381 gaggacagga gccgcagatg agccacgacc gcagagagtg cctgtgcctg cacgagaagc
     1441 cgatcaacga gctgcgtcgc ctcgtccatg ctcacccgtt ggaatctaat caatcgctca
     1501 acagccagga ccgtcccgcc cgaccgccgc gtggattcgc accgctagcc tcggaggtca
     1561 atgccacgtc ggattctcat cgccaagcca gggctcgatg ggcacgaccg gggtgccaag
     1621 gtcatcgccc gggcgctgcg cgatgccggt cacgaggtca tctactcggg cctccaccag
     1681 acgccggctc agatcgcgga gacggccgtg caggaggatg tcgatgccgt cgggctgtcg
     1741 tcgttgtcgg gagcccacaa gaccctcttc ccacaggtca tcgccgagtt ggccgatcgg
     1801 gactcgtcgg acatcatcgt gtttgggggc ggggtgatcc cggacgcaga caaggccgct
     1861 ctcgaggacg ccggcatcgt gatgatcttc acgccgggca ctccactcga cgagatcacc
     1921 ggctgggtcg atcggagcat ccccagccgt gcacaacaga gaagaggagc atgaagatcc
     1981 acgagcacca ggccaaacaa ctcatggcag caaggggcat tccagtcccg ttgggccggg
     2041 tggcccgatc ggtcgacgag gccgtggccg cggtgaggcc gttgatcgac gagtcgggca
     2101 acccggtggt cgtcctgaag tcccaaatcc acgctggagg gcggggtaag ggcacgttcc
     2161 gggagcatcc cgacgtgcga ggggtgaatg tgatcaccga tggcatccaa ggaggcccat
     2221 ctgcagccga ggaggcggtc agggagctgg ccaagaagat gctcggcagc accttggtga
     2281 cgattcagac cggggacgag ggaaagaagg tgaatcgact ctacgtggag caaggcatcg
     2341 acatcgatcg cgagctctat ttggcggttc tgctcgaccg tgcgacatcg cgcaacatcg
     2401 tcatggcgtc gaccgagggc ggggtggaga tcgagaaagt ggccgaagag accccggaga
     2461 agatcctgcg cgctcaaatc gacccagcta tcggcctggc cccgtttcag gccaatgagc
     2521 tcgtccacgg gctcggcatc gagggcgacg cagccaagaa cttcacccgg ttcgtgatga
     2581 acctcgccga tgcggccccc gagctcgata ccgatctcat cgagatcaac cctctcgtca
     2641 tcaccaagac cggtgaggtg atggcactcg acgggaagat gtccttcgac gacaacgccc
     2701 tctaccgcca ccctgacatc gccgacatgc gggacgagac ggaggaagac ccgctcgagt
     2761 tggcagccaa gaacgccggg ctcaactaca tcaagctcga cggcacgatc gggtgtttgg
     2821 tcaatggtgc cggtctggcg atggcaacca tggacaccat caagcacgtg ggcggcgagc
     2881 cggccaactt cctcgacgtc gggggcgggg ccaccgcaga ccaggtgacg acggcattcc
     2941 agatcatcac caaggatccc tcggtcaagg gcatattcgt caacatcttc ggtgggatca
     3001 tgcgctgtga tgtgatcgca accgggatcg tcgaggcggt ggagagggtg gggctggaag
     3061 tgccgctcgt ggtcagactg gagggtacga atgtcgagga gggcaaggac atcatcgaga
     3121 agtcggggct agaagtgacc aacgccgact cgctgcgtga cggtgccgag aagatcctgg
     3181 agctatcgac atgagtgtcc tcgtggacaa gagcactcgg gtcgtggtg
//
LOCUS       DASUTA010000086         3541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1067225, whole genome shotgun sequence.
ACCESSION   DASUTA010000086 DASUTA010000000
VERSION     DASUTA010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067225"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VFZ80_01680"
     CDS             complement(<1..540)
                     /locus_tag="VFZ80_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6286171.1"
                     /translation="MAQIFQSIEPESTPPQSRLLWRVTPPTIRGVGRVFFSYQIEREA
                     DLPPPPYVVAANHYSHFDAAVVGAAIDRPMRFLALEDLFGTNRLLDWLIVGFGAIPTP
                     RSRTPVSAVRTALRALENGDVVGLFPESTRVSHWGTLPPRRGAAWLARRVGVPIVPVA
                     VIGTGLAFGLENRLRRAPVR"
     gene            complement(571..1431)
                     /locus_tag="VFZ80_01685"
     CDS             complement(571..1431)
                     /locus_tag="VFZ80_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286172.1"
                     /translation="MPLLVGTETGAVTLDGDTLITGVRVNHMAQATDGWWAVDGSGRV
                     HRDGDVVAEMPPGVTAHCVQPTDQTTWIGADRATLFALDPSGMTEDEFFANAPGRDTW
                     YTPWGAPADVRSMAVDADHTLYINVHVGGVLRYDDTGVVPTLDVESDVHQVATHPSQK
                     GAVFAACAYGLAFSHNGHDFEIRSDGLHDGYCRAVTVLEDRVLVSASTGPRTDKARLY
                     RGELWSGQLEPLRSGLPEWFGSNLDTHCLIAREDGVFAGHGDTVWRSDDRGDTWDVAV
                     SGLDAITCLA"
     gene            complement(1514..2476)
                     /locus_tag="VFZ80_01690"
     CDS             complement(1514..2476)
                     /locus_tag="VFZ80_01690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007239232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX6286173.1"
                     /translation="MTRLGYQIPNFTYPGVGPDGLFEAIARQAREAEVSGFDTVMVMD
                     HFYQLPGLGEPDDYMLECYTLLAALSRETKSVRLGSLVSGNTYRNPAVLGKIITALDV
                     VSEGRALLGIGAGWYEIEHDSFGIEFGTFTDRFEKMEEALQLLLPMLDGNRPSLDGEH
                     YKFQDVINQPPPVGRIPVMIGGSGEKKTLRMVAQYADESNLIAAAKDIPRKLEALDEH
                     CERLGRDRSEITVSWLRSAVIAPTLDEAWSEASAWFDRRGFDDETRESVLSRYHVGDP
                     DTVAGMFKEDLATGVDGFTVNMPANGHIPDRVSLLGETLSPLFG"
     gene            2593..3132
                     /locus_tag="VFZ80_01695"
     CDS             2593..3132
                     /locus_tag="VFZ80_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286174.1"
                     /translation="METSEMSSFDARLRVPGHNKLPVNVVVDIADENLSVSTSQGKVG
                     DWPLSTLQFSARSDGFHVKLDDQEIVLSVDDSARFALELGTVGLHRTRTGISRRLSGI
                     TPDEQFEDLKRRIDEVAEAIEDESVSPERAFGQWLRLLKDINVHHGQGSMPTPLFYRL
                     NTRLLDLIPVPPRVRQPVG"
     gene            3245..>3541
                     /locus_tag="VFZ80_01700"
     CDS             3245..>3541
                     /locus_tag="VFZ80_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286175.1"
                     /translation="MPAVVGVFLERDETETAIDQLVALGVDADALGVVWREKTVGKYE
                     ETERIVYVDHFEDPGTEARKGAIGGAVGGGAAGVGSVLLATTGGVVIGTPIGALL"
BASE COUNT          651 a         1137 c         1114 g          639 t
ORIGIN      
        1 ccgaaccggc gcccgtcgca gccggttctc cagaccgaac gccaggccgg ttccgatcac
       61 tgccacaggg acaatcggaa caccaacccg cctggcgagc catgccgccc cacggcgggg
      121 tggcagcgtt ccccaatggg acacgcgcgt ggactcaggg aacagcccga ccacatctcc
      181 gttctcgagg gcgcggaggg cagttctcac cgcggatacc ggcgtgcggc tacgtggcgt
      241 cggaatcgcg ccaaatccga cgatcagcca gtcgagcagt cggtttgtcc cgaagagatc
      301 ctccaacgcc aggaacctca tcggtcggtc gatggcggcc ccgaccaccg ccgcgtcgaa
      361 atgggagtag tggttcgcgg ccaccacgta tggtggcggg gggagatccg cttccctttc
      421 gatctggtaa gagaagaaga cccggcccac gccccgaatc gtgggaggag tgactctcca
      481 cagcagccga ctctgtggcg gggtcgactc cggctcgatg ctctggaaga tctgggccat
      541 gggcaagtga tgggatgtcg attcgcttgg ctaagccaag caggtgatcg cgtccagccc
      601 tgagactgct acatcccatg tgtcgccgcg gtcatccgat cgccacacgg tgtcaccgtg
      661 cccggcaaaa acaccgtcct cccgggcgat cagacaatga gtgtcgaggt tcgacccgaa
      721 ccactcgggc agacctgacc tcaacggctc gagttgccca gaccagagct cgcctcgata
      781 gagcctggcc ttgtcggtgc ggggaccggt cgaagccgag accagcaccc gatcctcgag
      841 aacggtgaca gcccggcagt acccgtcgtg gagcccgtcg gagcggatct cgaagtcgtg
      901 accgttgtgg gagaacgcaa gcccgtaggc acaagcggcg aagacggctc ctttctggct
      961 gggatgggtg gccacctggt ggacgtcgga ctcgacgtcc agggtcggca ccactcctgt
     1021 gtcgtcgtaa cggaggacac ctccgacgtg cacgttgatg tagagggtgt ggtcggcgtc
     1081 gaccgccatg gagcgcacgt cggcgggggc gccccaaggc gtgtaccagg tgtctcttcc
     1141 cggagcgttg gcgaagaact cgtcctccgt catgccgctc gggtccaacg cgaacagcgt
     1201 cgcccgatcg gcaccgatcc aggtcgtctg atccgtcggc tgtacgcagt gggcggtcac
     1261 acccggaggc atctcggcga cgacatcgcc gtcgcgatgt acacgtcccg atccgtctac
     1321 agcccaccac ccgtccgtgg cctgggccat gtgattgacg cgcacaccag tgatcagcgt
     1381 gtcgccgtcg agagtgacgg cgccggtctc tgttccaacg aggagtggca cggtacgagc
     1441 ctacgccggg ccggggccag tcgagccacg ggtggtgttg ggtatagagg tactcagcgt
     1501 gaagccgggc tcctcatccg aacagggggc tcagcgtctc cccgaggagt gaaactcggt
     1561 ccggtatgtg accgttggcg ggcatgttga ccgtgaaccc gtcgactccg gtggcgaggt
     1621 cctccttgaa catgccggca acggtatccg ggtcacctac gtggtaacga ctgagcacag
     1681 attcccgcgt ctcgtcgtcg aagccgcgcc ggtcgaacca tgcgcttgcc tcgctccagg
     1741 cttcatccag ggtcggcgcg atgaccgcag accggagcca actgacggtt atctcagacc
     1801 gatcacgacc gagacgttca cagtgctcgt caagggcttc gagcttgcga gggatgtcct
     1861 tggccgccgc gatcagattc gactcgtcgg catattgggc aaccatgcgg agagtcttct
     1921 tctcgcctga tccaccgatc atcaccggta tccgcccaac cgggggcggc tggttgatca
     1981 catcctggaa cttgtagtgc tcgccatcca aggaaggtcg gttgccgtcg agcatcggga
     2041 ggagcagttg gagtgcttcc tccatcttct cgaatcggtc ggtgaaggtg ccgaactcga
     2101 tgccaaacga gtcatgctcg atctcatacc agccggcgcc gatgccgagc agggcgcgcc
     2161 cctccgacac cacgtccagt gcagtgatga tcttgccgag cactgcgggg tttcgatacg
     2221 tgttaccact caccagggat ccgagacgaa cgctcttggt ctccctggac agggcggcga
     2281 gcagcgtgta gcactcgagc atgtagtcgt cgggctcacc gaggccgggg agctggtaga
     2341 agtgatccat caccatgacg gtgtcgaaac cagagacttc ggcctctctg gcctgacgtg
     2401 caatcgcctc gaacagcccg tccggcccaa cccctggata ggtgaagttg gggatctgat
     2461 agccgagtct ggtcatgtcg ccaccctatc ccggtgcccg ggttggtcga gattttttca
     2521 tttaccccgt gtcctgatcg ctgtctgcag cgtttacacc actgtctggg tcgggctggg
     2581 tgaacgactg agatggagac aagcgagatg tcgtctttcg acgcaagact tcgtgtaccc
     2641 ggccacaaca agctccccgt caacgtggtg gtcgacatcg ccgatgagaa cctgtcggtc
     2701 agcaccagcc aaggcaaggt cggggactgg ccgctcagca cgcttcagtt ctccgctcgc
     2761 tccgacggct ttcacgtcaa gctcgacgat caggagatcg tgctcagcgt cgacgactcg
     2821 gctcgcttcg ctctggagct cggcaccgtc gggcttcatc gcacccggac cggaatctcc
     2881 cggaggctct ccggaatcac tcccgatgag cagttcgaag acctcaaacg acggatcgac
     2941 gaggtcgccg aagccatcga ggacgagtca gtctcccccg agcgcgcctt cggtcagtgg
     3001 ttgcgtcttc tcaaggacat caatgtccac cacggccagg gctcgatgcc aacccctctc
     3061 ttctaccgcc tgaacacccg ccttctcgac ctcatcccgg tccccccgag agtgcgccag
     3121 ccggtcggct agcccctcaa atcccgaacc cggggttcct caggccgtat acggcgcaaa
     3181 tcagctcatc ttcggctgac tggggccggt ttcggcaaca cacggcgacg ggtacattga
     3241 ccacatgccc gccgtcgtcg gagtcttcct cgaaagggac gagaccgaga ccgccatcga
     3301 tcagctcgtc gcgttgggtg tcgacgccga cgcgctcggc gtggtgtgga gggagaagac
     3361 agtcggcaag tacgaagaga cggagcgaat cgtctatgtc gaccatttcg aggatcccgg
     3421 caccgaagca cgcaaggggg cgatcggtgg tgccgtgggc gggggagcgg cgggcgtcgg
     3481 cagtgttctc ctcgccacta ccggtggtgt cgtcatcggc actccaatcg gagctctcct
     3541 c
//
LOCUS       DASUTA010000087         2125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1585788, whole genome shotgun sequence.
ACCESSION   DASUTA010000087 DASUTA010000000
VERSION     DASUTA010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585788"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VFZ80_01705"
     CDS             <1..1007
                     /locus_tag="VFZ80_01705"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6286176.1"
                     /translation="FTPTFRNLMALVALFAVTSAVVQSVLPVHTEYLGGGAAAFGILY
                     GAMGAGALLGAVIRPRISNRFTGSWVPVTIGLFGCAGILLGVAPNLWVAGLAMVLAGL
                     FWLLTLATLNATAQLMSPPWIRGRAMSIYTLAFAGVLPIGSILSGLVADAIGTTGSLL
                     VFSSGALLLGVMAPRFQVPHVDEVVTPEFSEDRHATPHDDGTFEGGPVIVLNTWKIAE
                     PDFAEFTSLMNEVRLVRLSTGAYRWRLFRSTSDPTRLTELMVLQSWEDHLAQHRRIDD
                     AAVSLLRRARAFDVEDGPRTRHLVAIDVENPPEFDDLVAAHERLHQVDGSIPRSDQEP
                     "
     gene            complement(995..1675)
                     /locus_tag="VFZ80_01710"
     CDS             complement(995..1675)
                     /locus_tag="VFZ80_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286177.1"
                     /translation="MTSTSVEESLDRAEAATSAGAGLSGTGFWSAVATVKRNPELVDR
                     HADRIAAIDEAAFRQWVLLTVPLGLGTALAVTALVVGIFLIGLAYALDGFPAVLVFFA
                     GFVAVIGSTHGLAHLVVGWAVGIRFTCWFIARVTQPQPGVKVDYASYLRTAPQKRAWM
                     HASGAIVTKAIPFMLIGAALAANLPTWAVWLLLVIGIVVLLADLVWSTKKSDWKKFQR
                     EMSFAQGS"
     gene            1739..>2125
                     /locus_tag="VFZ80_01715"
     CDS             1739..>2125
                     /locus_tag="VFZ80_01715"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX6286178.1"
                     /translation="MMDDLANALASDLDRAFPSVVEEMGRKLFWGLRRICGDHQEAED
                     LTQETFIRAYRALGTYETERIRTLNLQPWLWTIAMNLGRNHLRDRSRRPVWVDTDVDP
                     TETDPEPMDEVAWDRRLSSLNTHQRTA"
BASE COUNT          390 a          695 c          661 g          379 t
ORIGIN      
        1 gattcactcc gacgtttcgc aacctcatgg cgctggtcgc actgttcgca gtcacctctg
       61 ctgtggtcca gtcggtgctg cctgtgcata ccgagtacct tgggggaggg gctgcggcat
      121 tcgggatcct ctacggggcc atgggcgccg gagccttgct cggtgccgtc atcaggcccc
      181 gaatcagcaa ccgcttcaca ggctcgtggg tgccggtgac gatcgggcta ttcggatgcg
      241 ctgggatact gctcggagtg gcacccaacc tctgggtggc cgggttggcg atggtgctcg
      301 ccggcttgtt ctggctgctg accttggcga cgctgaacgc gacggcccag ctcatgtccc
      361 cgccctggat cagagggcgt gccatgtcga tctacacgct ggcattcgcc ggggttctcc
      421 ccatcgggtc gatcctctcc gggctggtcg ccgacgcgat cgggaccacc ggctctctgc
      481 tggtgttctc ctcgggcgcc ctgcttctcg gggtgatggc tccgaggttc caggtgccac
      541 acgtcgatga agtggtcacg ccggagttca gcgaggaccg ccacgcaacc ccccacgacg
      601 acgggacgtt cgagggcgga ccggtgatcg tgctcaacac ctggaagatc gccgagccgg
      661 acttcgccga attcaccagc ctgatgaacg aagtccgact ggtccggttg agtaccggtg
      721 cctatcgatg gcgactgttc cggagcacct ccgatcccac ccgtctcacc gagctgatgg
      781 tgctgcaaag ctgggaggac cacctggccc agcaccgtcg catcgacgac gcagccgtct
      841 cactcctccg cagagccagg gccttcgatg tcgaggacgg gccccgcacc cgccatctgg
      901 tcgcgatcga tgtcgagaac cccccggaat tcgacgacct ggtcgccgct cacgaacggc
      961 tccatcaggt ggatggctcg atcccgcgat cggatcagga gccctgagcg aaggacatct
     1021 ctctctggaa cttcttccag tcagacttct tggtcgacca gacgagatcg gctagaagaa
     1081 ccacgattcc gatcacgagc agcaaccaaa ctgcccaagt cgggaggttc gccgcgaggg
     1141 cggcacctat gagcatgaag ggaattgcct tcgtgacgat ggcaccgctg gcgtgcatcc
     1201 aggctctctt ttgcggcgca gtcctcaggt aggacgcata gtccaccttc acgcccggct
     1261 gcggttgagt caccctggcg atgaaccagc aagtgaaccg gatgccaacc gcccacccga
     1321 caacgaggtg ggccaatcca tgggtcgagc cgatcacagc aacgaatccg gcgaagaaga
     1381 cgaggaccgc gggaaagccg tcgagagcgt acgccagccc gatgaggaag atccccacca
     1441 ccagagccgt cacagccagt gcggtgccga ggccgagcgg aaccgtgaga aggacccatt
     1501 gccggaacgc tgcctcatcg atggcggcga ttcggtcggc atggcggtcg accaactccg
     1561 gatttcgctt cacggttgcc acggccgacc agaacccggt gcctgagagc cccgctccgg
     1621 ccgatgtcgc tgcttctgcc cggtccaggc tctcctcgac actggtcgat gtcacgccca
     1681 caaatatagg tacggtgccg accgtgcaac cggatgaccc gatccggggt tcttcttcat
     1741 gatggacgac ctggccaacg cccttgcctc cgacctggac cgagcattcc cgtcagtggt
     1801 cgaggagatg ggaagaaagc tgttctgggg cctccgtcgt atttgcggcg accaccagga
     1861 ggcggaagat ctgacgcaag agacctttat tcgcgcgtac cgcgctctgg gcacctacga
     1921 gacggagcgc attcggacgc tgaacctgca gccctggctg tggaccatcg ccatgaatct
     1981 ggggcgaaat cacctgcgtg atcgatcgcg acgcccggtg tgggtcgata cggacgtcga
     2041 ccccaccgaa accgaccccg agccgatgga cgaggtggca tgggatcgcc gtctctcgtc
     2101 tttgaacacc catcagcgga cagcc
//
LOCUS       DASUTA010000088         6399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_807663, whole genome shotgun sequence.
ACCESSION   DASUTA010000088 DASUTA010000000
VERSION     DASUTA010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807663"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..559)
                     /locus_tag="VFZ80_01720"
     CDS             complement(113..559)
                     /locus_tag="VFZ80_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286179.1"
                     /translation="MPAYDGVVIVAEDDIPVIVELGDDVVRLSASGREIGEWRTGEYE
                     IRHIGESTFAIQAENEILEFVPSQPTDFAAAVNGGLPRTELKPADEPSAVPPPAASEP
                     DPGSEPDPKSEAGEAPPPTATTLGLFYALCLVTVALGIWAVISLIV"
     gene            complement(634..1329)
                     /locus_tag="VFZ80_01725"
     CDS             complement(634..1329)
                     /locus_tag="VFZ80_01725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02453 family protein"
                     /protein_id="HEX6286180.1"
                     /translation="MPRYFTPAVFAFLRELADNNNKEWWEGNKDRYVETIREPARDFI
                     ADFGGRLAEISPHFSADTRTNGGSLMRPYRDMRFASDKTPYKTNVGIQFRHKRGKDVH
                     APGFYLHLEPGRSFAGAGMWSPEPRVARRIRQAISDEPREWERAAHSQSFTSNWAIGE
                     HDEERLRRVPRELNGNGHPYPEDLRLKSFTAGAKLSQKLVTSEHFPDELLALYVSAGP
                     YTKFLCEAISVEF"
     gene            complement(1395..2726)
                     /locus_tag="VFZ80_01730"
     CDS             complement(1395..2726)
                     /locus_tag="VFZ80_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HEX6286181.1"
                     /translation="METHQVHTRSALLFMGAAAVVSIPGVVLRLAFDHPNPILGSILF
                     GMAIVGAAFMISWAAEIIQLDVGGGLALALLALIAVLPEYAVDFVLTAEAGRVFAETG
                     AEDAGQLALANMTGANQLLIGFGWPLVILLGTWRVRRTGASPAVAEMATGDSVNLTRT
                     QNVDIAYLAIASLYGMTLFLKSTLSPFDAVVLVSIYVLYMFRLSGATPGEPHLVGPAA
                     YIGSMPKVRRRVINYIMFAFSAAVIVLVAHEFAVSLQELGEAIGISEFLLIKWLAPLA
                     SEAPELIVATLFAWRLAARTGIGALISSKVNQWTLLVGTLPIVFMIFAGQVHGLPLDD
                     VQRDELWVTATQSVFAVAIIASRSISTREAWAMFALFCVQLGESWFAEAGIISPGVAE
                     AARIGVGVMFLLAAVFVLRKDFRRFGRAIKDGLRTPWSELESDTESPESVA"
     gene            complement(2750..3919)
                     /locus_tag="VFZ80_01735"
     CDS             complement(2750..3919)
                     /locus_tag="VFZ80_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX6286182.1"
                     /translation="MRFARLFTALLALSLLASIVTTGGPLARAQSTDEAEQDAERAEQ
                     RADTASGLVDEAVANRDALEHQLADSIARMNELAAQLSEVGSALDGLAAQVGYADIEL
                     AGIQADIEDQAVDAYMTVVASPTASLVRTSTVEMAMVASSVVEDVVADGRVTVGELFA
                     KRKDLESLKEAYLANQEEYQALKAEVDAEVERYAALYDEAEADVADAIRVAEQANQDY
                     IAALSAVDLAKAKDDERKRQEARDDGTTTTTGPGTTSPSTTTASSPTTTTATTSTTSG
                     GGGGGPWSHPPEVEQWRGLVSAYFPSDRVEEALRIIGCESNGDPNALNPYSGAAGLFQ
                     FLSSTWESTSPKAGFEGASVFDPEANIGSAAWLANRYSQLGYDYWHPWSCKRVLG"
     gene            3987..4370
                     /locus_tag="VFZ80_01740"
     CDS             3987..4370
                     /locus_tag="VFZ80_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6286183.1"
                     /translation="MTRWNVSSGSPFEPRYGFSRAVRVGARVIVAGTAPIWPDDFVDP
                     DPGAQAARCLEIMLDALEEAGGQVGDVVRTRMFITDPDHADAIGDAHGEVFSEIRPAS
                     TMVVVAGLLDPRWHVEMELEAVLTR"
     gene            4438..5076
                     /gene="msrA"
                     /locus_tag="VFZ80_01745"
     CDS             4438..5076
                     /gene="msrA"
                     /locus_tag="VFZ80_01745"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019872860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX6286184.1"
                     /translation="MFGRSAKPMPAPENALPGRSESVDVPTAHFVNGNPLVGPFPEGT
                     EVAVFGMGCFWGAERRFWRMPGVFTTSAGYAGGSTPNPTYEEVCSGRTGHAEVVQVVY
                     DPSQVTYDELLRVFWETHDPTQGYRQGNDVGTQYRSTIYTTTDAQLDQAKRSKDIYQS
                     VLTEKGFGDITTEIEPLGEYYFAEDYHQQYLGKNPHGYDCHANTGIAYPGLD"
     gene            5199..5579
                     /locus_tag="VFZ80_01750"
     CDS             5199..5579
                     /locus_tag="VFZ80_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX6286185.1"
                     /translation="MRNSVLWILQWLFGLYFIAIGVMHFIVPEGLPDQLEWMYDLSDP
                     LHMVTGVAEVLGGLGLILPSLTRIQPSLTPIAAVGLIAVMAGAVVWHFGREEYVNIGL
                     NVVNAVALAYIAYGRARLEPIPPR"
     gene            complement(5583..6374)
                     /locus_tag="VFZ80_01755"
     CDS             complement(5583..6374)
                     /locus_tag="VFZ80_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX6286186.1"
                     /translation="MIVVSTIEGAPADDAGLMGEDEIVAVNGESIIGWTVDEVTAMVR
                     GRSGTDVTLTIDRAGDRFDVTITRAAVQIPVIADEVIGDVGYLRLWNFTGSASSQFES
                     AIVDLLGQGVEELVIDLRDNPGGFLTTAIDVTSVFVDDGDVVVTEGPNDDNTYPVSGT
                     AIVPEDMEVTFVVNRGSASASEVVAATLQERGRVTVIGESTFGKNTVQQRFSLSNGGA
                     LKLTIARWLTPGGLDFGGTGVTPDVEIENVSSLTTAEVVAAATDA"
BASE COUNT         1198 a         1982 c         2045 g         1174 t
ORIGIN      
        1 gacatactca tgggtcggga acagctctgc gacgtgctcg atcgagcgcg ggaggtgagc
       61 gagtacctgc tcgaccgggt gagcggctgg atggagaccc cggggagctg aatcagacga
      121 tgaggctgat gaccgcccaa atgcccagtg cgaccgtgac caggcacaac gcatagaaca
      181 agcccagcgt cgtcgctgtc ggaggcggtg cctctccggc ctcggatttg gggtcgggct
      241 ccgaccctgg atctggctcg gaggcggcgg gcgggggaac agcggaaggc tcgtccgccg
      301 gcttcagttc ggtcctggga agaccaccgt tgacggcggc ggcaaaatcg gtcggctggc
      361 tcgggacgaa ctccaggatc tcgttttcgg cctgaatggc aaacgtggat tccccgatgt
      421 gacgaatctc gtattccccg gttctccatt ccccgatctc cctgcccgaa gccgacagcc
      481 ggacgacgtc gtcgcccagt tcgacgatca ccggaatgtc atcctccgca acgatcacca
      541 cgccgtcata ggcaggcatc ttcggctccc cattggatag gccactgaca aattaggcgc
      601 ttgcgagcag tgtgtcgacc gaaagccgac gacctagaac tccactgaga tcgcctcgca
      661 gagaaacttc gtgtacggcc cggccgagac atagagggcg agaagttcat cggggaaatg
      721 ctcagaggtg acgagcttct gagacagctt ggcaccggcc gtgaagctct tcaggcgaag
      781 atcctccggg tagggatggc cgttgccgtt caactccctg ggcactctcc gcaggcgttc
      841 ctcgtcgtgc tctccgatcg cccaattgct ggtgaaggat tgggagtgcg ctgcccgctc
      901 ccactctctc ggctcgtcgc tgatggcttg gcgaattcgc cgggcaaccc tcggctccgg
      961 ggaccacatg ccggcgccgg caaagctacg tcccggctcg agatggaggt agaagcctgg
     1021 cgcatggacg tcctttccgc gcttgtgcct gaactggatg ccgacattcg tcttgtaggg
     1081 cgtcttgtcg ctggcgaatc gcatgtcgcg gtagggacgc atcagcgaac ccccattggt
     1141 gcgggtgtct gcggagaagt gtggtgatat ctcggccaaa cggccaccga agtcagctat
     1201 gaagtcgcgg gccggttctc tgatcgtctc gacgtagcga tctttgttcc cttcccacca
     1261 ttccttgttg ttgttgtccg ccagctcgcg caggaacgcg aacaccgcag gcgtgaagta
     1321 tctgggcatc catctctcct ttcacccata tgacgaacat acggtgaccg atcgacagat
     1381 ttgaaggggg ttgatcaggc gacggactcc gggctctccg tatcggactc gagctcggac
     1441 cagggcgtgc gaagaccgtc cttgatcgcc cggccgaaac gtctgaagtc cttgcggagc
     1501 acgaagaccg cagcgaggag gaacatcact cccaccccga tgcgggctgc ctcagcgacc
     1561 cccggggaga tgatgccggc ctcggcaaac cacgactcgc ccaactggac gcagaagagg
     1621 gcgaacatgg cccaagcctc tctggtgctg atcgaccggc tggcaatgat ggcgacggcg
     1681 aacaccgatt gagtggctgt gacccagagc tcgtcccttt gtacgtcgtc caacggcagg
     1741 ccatggacct ggccggcgaa gatcatgaac acgatgggca acgttccgac cagcagagtc
     1801 cactggttga ccttcgagga gataagggca ccgatccccg tcctggcggc gagacgccag
     1861 gcgaacaacg tggcgacgat gagttccggc gcctcggagg cgaggggcgc cagccacttg
     1921 atgaggagga actcgctgat cccaatggcc tcaccgagtt cctgcagcga gacggcgaat
     1981 tcgtgggcga cgaggacgat cacggcggcc gagaaggcga acatgatgta gttgatgacg
     2041 cgtctgcgga ccttgggcat cgagccgatg taggcggcgg ggccaacgag atgaggctca
     2101 cccggcgtgg ctccggatag tcggaacata tacaggacat agatcgagac caggacgacg
     2161 gcgtcgaatg gtgacagagt gctcttcaag aagagggtca tcccgtatag ggaggcgatg
     2221 gcgaggtacg cgatgtccac gttctgagtc ctggtcaggt tcacgctgtc gccggtggcc
     2281 atctcggcca ctgcgggcga ggctccggtc cggcgcaccc gccaagtccc gagcaggatg
     2341 accagaggcc agccgaatcc gatgagaagc tggttggctc cggtcatgtt ggccaatgcc
     2401 agctggcccg catcctccgc gcccgtctcg gcgaaaaccc gaccggcctc ggcagtcagc
     2461 acgaagtcga cggcgtactc gggcagaacc gcgatcaagg ccaaaagcgc gagagcgagc
     2521 ccacccccga catcgagctg gatgatctcc gcagcccagg agatcatgaa ggctgcgccg
     2581 acgatggcca tcccaaacag gatggacccc aggatcgggt tgggatgatc gaaggcaagg
     2641 cggagcacga caccagggat ggaaaccacg gcggcagccc ccatgaacaa gagtgcgctt
     2701 ctcgtgtgga cttggtgagt ctccattgcc gggcgagtct aagtggctgt cagccgagta
     2761 cccgtttgca gctccacggg tgccagtagt cgtagccgag ctggctgtag cgattcgcca
     2821 gccacgccgc cgagccgatg ttcgcctcgg ggtcgaacac gctcgctcct tcgaagcccg
     2881 ccttgggcga ggtggactcc caggtggaag ataggaactg gaacagcccg gcggctcccg
     2941 aatacgggtt gagggcgttg ggatcaccgt tcgactcaca gccgatgatc cggagagcct
     3001 cttcgacccg atcggaaggg aagtacgccg agacgagccc ccgccactgc tcgacctcgg
     3061 gtggatgact ccaggggccg ccacctcctc caccagaagt ggtcgaagtc gtggcggtgg
     3121 ttgtggtagg gctggacgca gtggtggtcg aagggctggt cgtgcccggg cccgtggtgg
     3181 tcgttgtgcc gtcgtcgcga gcctcttgac gttttctctc atcgtccttc gccttggcga
     3241 gatcgactgc gctgagagcg gcgatgtaat cctggttggc ctgctcagcc acccggatcg
     3301 catcagcgac gtcggcctct gcctcgtcat acaaagccgc gtagcgctcc acttcggcat
     3361 ccacctccgc cttgagcgcc tggtactcct cctgattggc caagtacgcc tctttcaggc
     3421 tctcgaggtc ctttcgcttg gcaaagagct cgccgaccgt gacccgaccg tcggcaacga
     3481 cgtcttccac gaccgaactg gccaccattg ccatctcgac cgtcgacgtc ctcaccagcg
     3541 aagcagtagg agaggccacc accgtcatgt aggcgtcgac tgcttggtcc tcgatatcgg
     3601 cctggatgcc ggccagctct atgtccgcat acccgacctg agccgccaaa ccgtccaagg
     3661 ctgagcccac ctcggagagc tgggctgcga gctcgttcat gcgggcgatc gagtccgcaa
     3721 gctggtgttc cagagcatcc cggtttgcca ctgcctcgtc gaccaggccc gaggcagtgt
     3781 cggccctctg ctcggctcgc tccgcgtctt gttccgcttc gtcggtcgat tgggcgcggg
     3841 ccaggggtcc cccggtggtc acgatggagg ccaggaggga cagagcgagc aatgccgtga
     3901 agaggcgagc gaaccgcatg gggtcatgtg agggtaacca accgcaggtc atccgagaag
     3961 tgggaaacgg ggttaccgtg tccccgatga cccgttggaa cgtctcatcg ggatcccctt
     4021 tcgagccgcg ttacgggttc agccgggcag tccgggttgg agcaagggtg atcgtcgccg
     4081 ggacagcgcc gatctggccc gacgacttcg tcgaccccga cccgggagct caggctgctc
     4141 ggtgtctcga gatcatgctc gatgctctcg aagaggccgg cgggcaagtc ggcgatgtgg
     4201 tcaggaccag gatgttcatc accgatcccg accacgctga cgccataggc gacgctcacg
     4261 gagaggtgtt ctcggaaata cgacctgcct cgacgatggt tgtagtagct ggactgttgg
     4321 atccgagatg gcacgtcgag atggagcttg aagccgtttt aacaaggtaa gagccattct
     4381 gtgagataga ccggcccttg gccgataggc ccacagaagg ttctcaggag gttcccgatg
     4441 tttggaagaa gtgcaaagcc gatgccggca cccgaaaatg ccctgcccgg tcgatcggag
     4501 agcgtggacg tgccgaccgc tcacttcgtc aacggaaacc cattggtggg cccattcccg
     4561 gagggcaccg aggttgccgt gttcggcatg ggctgtttct ggggtgcgga acgccggttc
     4621 tggcgcatgc ccggcgtgtt cacgacctcg gccggctacg ccggggggag cactcccaat
     4681 ccgacctacg aggaagtgtg ctcgggtcgc accggacatg ccgaggtagt ccaggtggtc
     4741 tatgacccgt cccaggtgac ctacgacgag ctgctgcgtg tcttctggga gacccacgat
     4801 cccacccagg gctaccgcca gggcaacgac gtcgggaccc aatatcggtc gaccatctac
     4861 acgacgaccg acgcgcagct agatcaggcc aagcgttcca aggacatcta tcagtcggtc
     4921 ttgaccgaga agggctttgg cgacatcaca accgagatcg agcccctggg cgagtactac
     4981 ttcgccgagg actaccacca gcagtatctg gggaagaacc ctcacgggta cgactgccac
     5041 gcaaacaccg gcatcgccta cccgggtctc gactgagcgg gccgtttcgg cgtgcccgtg
     5101 agcaggacgt tgcgctgcgc gcaacgcagc gcactcggag tcgagtgaga gccgacgccg
     5161 agggaaccga accggagtca gtcacgtagg ttgtcgatat gcgcaatagc gtgctctgga
     5221 tcctgcagtg gctgttcggc ctgtatttca tcgcgatcgg ggtgatgcat ttcatcgtgc
     5281 cggaagggct tcccgatcag ttggagtgga tgtacgacct gagcgaccct cttcacatgg
     5341 tcaccggggt ggcggaggtc ctgggtggtc tcggcttgat cctccccagc ctcacccgca
     5401 tccaaccttc gttgacgcca atcgcggccg tcgggctcat cgccgtgatg gcaggcgcag
     5461 tggtttggca tttcgggcgc gaggagtacg tcaacattgg cctcaacgtc gtcaatgcgg
     5521 tggcgctggc gtacatcgca tacggtcggg cccggttgga gccgatccca ccgaggtgac
     5581 ggtcaggcgt cagtggccgc cgcgaccacc tctgcggtcg tcaagctgga cacgttctcg
     5641 atctcgacgt caggcgtcac gcctgttccg ccgaagtcga gcccacccgg agtcagccag
     5701 cgggcaatgg tcagcttcag ggctccgcca tttgacaggc tgaagcgctg ctggacggtg
     5761 ttcttgccaa aggtgctctc tccgatgacg gttacccggc ccctctcctg cagtgtcgct
     5821 gcaacgacct ccgatgcaga tgcgctgcct ctgttgacga cgaaggtcac ttccatgtcc
     5881 tcggggacga tcgcggtgcc actcaccgga taggtgttgt cgtcgttggg gccctcggtc
     5941 acgaccacgt ctccgtcgtc gacgaacacc gaagtcacat cgatcgcggt ggtgaggaaa
     6001 ccgcccggat tgtccctgag atcgatcacg agctcctcga caccttggcc aagcagatcc
     6061 acgatcgccg actcgaattg tgagcttgcc gagccggtga agttccacag gcggagatag
     6121 ccgacgtcgc cgatgacctc gtccgcgatg accggaatct gtactgcggc gcgggtgatg
     6181 gtcacatcga agcggtctcc agctcggtcg atggtgagag tgacgtctgt cccggagcgg
     6241 ccccgaacca tggccgtcac ctcgtcgacc gtccatccga tgatcgactc gccgttgact
     6301 gccacgatct cgtcctctcc catcagtccg gcgtcgtcgg ccggtgcccc ctcgatcgtc
     6361 gagacgacga tcatctcgca tgtctacgat atcacaccg
//
LOCUS       DASUTA010000089         3749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1327007, whole genome shotgun sequence.
ACCESSION   DASUTA010000089 DASUTA010000000
VERSION     DASUTA010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327007"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFZ80_01760"
     CDS             complement(<1..452)
                     /locus_tag="VFZ80_01760"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286187.1"
                     /translation="MKDLLAYLGLRAGVGLVGALPQGAVRGLGRAFGSVWGLLDSDRR
                     SMGKRHLDRVLDNGTNTDTRSKEMMRAYGRYYAEALWVRERRVPEMLADTEVEGLEHI
                     LAARDEGRGMIYALPHMGNWEAAAPVAVREGIPVVAVAEVLPNRRITD"
     gene            complement(449..1057)
                     /locus_tag="VFZ80_01765"
     CDS             complement(449..1057)
                     /locus_tag="VFZ80_01765"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX6286188.1"
                     /translation="MWTRFPGGCYFLAVIDMRVRPHVTRYLDVMGRGLSKIGMTPTAM
                     TALGLALVITGSVMVGMGRLVSGAWVIAAGSLLDGLDGAVARASGKVTARGAFLDSAF
                     DRIGEIAAFAGLAFAMEGQARVLLLIVLAVGGAMLVPYMRARAEAEGLPGQGGLMGRT
                     ERLILFCTGLVFGFVEVMLWIFVVLVWLTALSRFLRSYRSIT"
     gene            1096..3150
                     /gene="fusA"
                     /locus_tag="VFZ80_01770"
     CDS             1096..3150
                     /gene="fusA"
                     /locus_tag="VFZ80_01770"
                     /inference="COORDINATES: protein motif:HMM:NF009891.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX6286189.1"
                     /translation="MVPEKVRNIALVGHGGSGKTTLAEALLFVGGAVNRMGSVEKGTT
                     TFDYEPEEIDRGISLGLAVATADWNGTRVNIIDTPGATEFSGDARSALRASDLALFAV
                     SAVDGVEVQTEALWRVAEEEGIPRAIVITKLDRERASYERVLEELRNSFGKSVAPIQV
                     PLGSEDNLRGVVRVATERAYEYSEGSSKGHQVDLPDEVAELVHAAHTGLIETVVETDD
                     DMMEAYFEGTEPSREQIVDTIHHGIVSGEIHPVLVTSAVKLIGIDLLFEFIVDYGPNP
                     LERSIPPLQRGASLEPTTVGPVLAYVFKTISDPFVGRISLFRIYSGTAKGDQELELAR
                     GGKVRLHNLFKLQGKDHHDVAELPVGGIGAVAKVEDLHPGDTLRSPGIDAVIEPVKYP
                     PSVTELAIAPRSHHDDEKMSTALHRIEEADPTIRIERRSETGETIMSGLGDTHLDVTL
                     ARIKRMYGVEIDATIPRVPYRESIMATAEAEGKHKKQSGGRGQFGVAMVRFAPKPRGT
                     GYEFIDSIKGGSIPRGLIPAVDKGIQEALGRGILAGFPVVDVSAELYDGKYHAVDSDE
                     MSFRMAGIQAVRTAAGKLRPVLLEPIMRVSVTVPEDHLGDVMGDINSKRGKVLGMEGE
                     GMLRTVNAEVPMSEMQQYAAELRSLTSGRGTFEMEFDHYEEVPHNEAQAVIAAQSDDD
                     QG"
     gene            3164..>3749
                     /locus_tag="VFZ80_01775"
     CDS             3164..>3749
                     /locus_tag="VFZ80_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX6286190.1"
                     /translation="MPEAVLHYGDEELTLAIEESTEGDTGVDISALRSSLGMVTLDRG
                     FANTAEGTSKITYINGEEGILRYRGYPIEQLAEQASFLEVAYLLQYGELPTRAELEAY
                     EESITHHTLLREDMKHLFEAFPHEAHPMQILASATSAMATYYPDALDPLDIDAVDISS
                     RRLIAKTPTMIAWSYKYSAHQPYVYPDNSLDYSSN"
BASE COUNT          800 a         1220 c         1117 g          612 t
ORIGIN      
        1 caatcggtga tgcgccgatt tggcagcacc tcggcaactg caaccaccgg gatcccctct
       61 cgaacggcaa ccggagcagc agcttcccaa ttgcccatgt gtgggagcgc atagatcatg
      121 ccccttccct cgtcgcgggc ggcgaggatg tgctcgagcc cttcgacctc ggtgtcggcg
      181 agcatctcgg gcacccgacg ctcccgcacc cacagagcct cggcgtagta gcggccatag
      241 gctctcatca tctctttgga ccgggtgtcg gtgttggtcc cgttgtcgag aacgcgatcc
      301 agatgcctct tgcccatcga tcgccgatcc gagtccaaga ggccccagac gctcccgaaa
      361 gccctgccca acccgcgcac agctccctgg ggcagcgcgc caacaagccc gacaccggct
      421 ctgaggccga gatatgccaa caagtccttc acgtgatgga acggtaagag cgtagaaaac
      481 gactcagtgc cgtgagccag accaagacga cgaagatcca gagcatcacc tcgacgaatc
      541 cgaaaacgag acccgtgcag aacaggatca agcgctcggt tcgtcccatg agaccaccct
      601 gtcccggaag tccctcggct tcggcgcgag ccctcatata gggcaccagc atcgccccgc
      661 cgacggcgag gacgatgagc aagagcactc ttgcctggcc ttccatggcg aatgcgagac
      721 cggcgaacgc cgctatctcc ccgattcgat cgaacgccga atcgagaaag gctccacgcg
      781 cagtgacctt gcccgaggcg cgagcaaccg ccccatcgag gccatcgagg agcgaccctg
      841 ccgcaatcac ccaggcgccg gagacgagcc tgcccatacc gaccatcacc gaacccgtga
      901 tgaccagcgc cagccccaga gcggtcattg cagtgggggt catcccgatc ttggaaagac
      961 cccggcccat gacatccaga taccgggtca catgaggtcg gacccgcatg tcgatcacgg
     1021 cgagaaagta gcacccccct ggaaacctcg tccacatcca gggttaggat cggtcttttc
     1081 cgagaaggag gtcccgtggt cccagaaaag gtcagaaaca tcgccctggt cggtcatggc
     1141 ggcagtggaa agaccactct ggccgaagcg ctgctgttcg tcggaggcgc cgtcaaccgc
     1201 atgggctcgg tcgagaaggg caccacgact ttcgactacg agcccgagga gatcgatcgg
     1261 ggcatttcgc tgggtctggc cgttgccacc gcagattgga acgggaccag ggtcaacatc
     1321 atcgacacgc cgggggccac ggagttcagc ggcgacgctc gcagcgccct gagggcctcc
     1381 gacctcgccc tgttcgcggt ctcggcagtc gatggcgtcg aagtgcagac cgaggccctt
     1441 tggcgggtgg ccgaagaaga gggaattccc cgggcgatcg tcatcacaaa gctcgaccgc
     1501 gagcgtgcct cctacgagag agtcctcgaa gagctgcgaa acagcttcgg gaagtcagtc
     1561 gcacccatcc aggtcccact cggttcagaa gacaacctgc gcggcgtggt ccgcgtcgca
     1621 accgagcggg cttacgagta ttcagaaggc tcgtcgaagg gccaccaagt cgacttaccg
     1681 gacgaggtcg ccgaactcgt ccacgcagct cacaccggtc tcatcgagac cgtggtcgaa
     1741 accgacgacg acatgatgga ggcctacttc gagggcactg agcccagccg ggaacagatc
     1801 gtcgacacca tccaccatgg catcgtcagc ggagagattc acccggtcct ggtcacctcg
     1861 gcggtcaagc tgataggcat cgacctcctc ttcgagttca tcgtcgatta cgggcccaac
     1921 ccgttggagc ggtcgatccc gcccctccaa cgcggggcta gcttggagcc gacaacggtc
     1981 ggtcccgttc tcgcctacgt cttcaaaacc atctccgacc cgttcgtagg gcgtatctcc
     2041 ctgtttcgca tctacagcgg cacggcgaaa ggcgatcagg agctggaact ggcgaggggc
     2101 ggcaaagtca ggctgcacaa cctcttcaag ctccagggca aggaccatca cgacgtggcg
     2161 gagctccctg ttgggggtat cggtgccgtg gcaaaggtcg aagacctcca ccccggtgac
     2221 acgcttcggt ctccaggaat cgacgcggtc atcgagcctg tcaaataccc gccctcggtg
     2281 accgagctcg ccatcgctcc acgttcgcat cacgacgacg agaagatgtc gaccgcgctg
     2341 catcgcatcg aggaggccga ccccacgatt cgaatcgaac gtcgctcgga gaccggggag
     2401 acgatcatgt cgggactggg agacacccat ctcgatgtca ccctcgcccg catcaagcga
     2461 atgtacgggg tcgagatcga cgccacgatc ccgagagtgc cgtaccggga atcgatcatg
     2521 gcgacggcgg aagccgaggg caagcacaag aagcagtctg gaggtcgcgg ccaattcggt
     2581 gtggccatgg tccgcttcgc cccgaaaccc cgaggaaccg gctacgagtt catcgattcg
     2641 atcaagggcg ggtcgatacc acggggcctg ataccggcgg tggacaaagg gattcaggaa
     2701 gctctcggac gcggaatact cgccggcttt ccggtggtcg acgtctccgc cgagttgtac
     2761 gacggcaaat accacgcggt cgactccgat gagatgtcgt tccggatggc cgggatccag
     2821 gcggtgcgca ctgccgccgg caagcttcga ccggtcctgc tcgagccgat catgagagtg
     2881 agcgtgaccg tccctgagga tcatctcggc gatgtgatgg gtgacatcaa ctcaaagcga
     2941 ggaaaggttc tcggcatgga gggtgagggg atgctccgca ccgtgaacgc cgaggtcccc
     3001 atgtccgaga tgcagcaata cgccgccgaa ctgcgatcat tgacttctgg tcgaggtact
     3061 ttcgagatgg agttcgacca ctacgaggag gtgccgcaca acgaggccca ggccgtgatt
     3121 gcggcgcaat ccgacgacga ccagggatag agagaggaac gagatgcccg aagccgtcct
     3181 ccactacgga gatgaagaat tgaccctcgc catagaggag agcaccgaag gcgacaccgg
     3241 cgtcgacatc tcggctctgc gctcgtcact cgggatggtc actctcgatc gcggtttcgc
     3301 aaacaccgcc gaaggcacgt caaagatcac ctacatcaat ggtgaggagg ggatcctccg
     3361 ctaccgcggc tatcccatcg agcagttggc ggaacaggca tcatttctcg aggtcgccta
     3421 cctccttcag tacggggagt tgcccaccag ggccgagctc gaggcctacg aggagtcgat
     3481 cacccaccac accctcctgc gcgaggacat gaagcacctc ttcgaggcct ttccccacga
     3541 ggcgcatccg atgcagatcc tggcctcggc cacttcggcg atggcgacct actaccccga
     3601 cgcactcgac ccgctcgaca tcgatgcagt ggacatcagc tcgagacgtt tgatagccaa
     3661 gactccgacg atgatcgcct ggtcctacaa gtactcggcc catcagccct acgtctaccc
     3721 ggacaattcg ctcgactact cgtccaact
//
LOCUS       DASUTA010000090         2591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_937774, whole genome shotgun sequence.
ACCESSION   DASUTA010000090 DASUTA010000000
VERSION     DASUTA010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937774"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFZ80_01780"
     CDS             <1..346
                     /locus_tag="VFZ80_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6286191.1"
                     /translation="PFIDAARVSGGSNMHLILKHVIPNVLPLSFLYMMFTVTEAVSLE
                     ATLSFFGLIEAPMTWGIMLSTAQTQGYLRSGLEFWWLLIPAGLAVSLLAAGFFFAGRA
                     MDEVINPRLRAQ"
     gene            343..1398
                     /locus_tag="VFZ80_01785"
     CDS             343..1398
                     /locus_tag="VFZ80_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286192.1"
                     /translation="MTDTRLNTAEGNLETPVPNGEVPILEVKNLVMNYEMKSGMVSAV
                     EGVSFKLPKGKSLGLVGESGCGKTSVALTLMQLTASNARVLDGEIKLDGENLLELSDE
                     EMRKRRWNDVSMVFQGAMNSWNPVYTVGEQIRETMDIHWDPKPTTAQARAKIEELFTL
                     VGINPEMIDRYPHEFSGGMKQRAVIAMALACDPKLIIADEPTTALDVIVQEQILEELR
                     KIQDELGMSIIYISHDIAVIAEITNLIGVMYAGKIVELGPTTEIFANPRHPYAWLILS
                     STPSVTGPRRVLAPLEGEPPNLLDPPSGCRFHPRCPFATQKCADEEPPLADIEPGHAV
                     ACWHHDQVPATLAKVEA"
     gene            1395..2489
                     /locus_tag="VFZ80_01790"
     CDS             1395..2489
                     /locus_tag="VFZ80_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286193.1"
                     /translation="MTTDQSTTTERTGPKPNGEVRVKVDNLRKLFPISRGLFRAPTDF
                     VHAVDGVNFQIRAGESLGLVGESGCGKTTTGRMLVKLEDATDGSILIRDSESDELVNV
                     THINSKADQKVFRRNVQMIFQDPYESMNPRRTIFDIVAEPLTVQNLGTTSEREERVVD
                     LLRLVGLTPPEPFLFRYPHELSGGQRQRVAIARALVMDPTFVVADEPTSMLDVSIRIS
                     IMRLMLELAEKLGVTYLYITHDLAVARYMCDRIAVMYLGKIVEMGPTEDLLSNPMHPY
                     TRALLSAVPVPDPSYRRPPVEIEGGVARAINPSAECRFIARCPFATDICATEHPPLDD
                     RGDEHYVACYLVDAQGNWTGPKRAELDLRS"
BASE COUNT          493 a          858 c          772 g          468 t
ORIGIN      
        1 gccattcatc gacgccgccc gggtctctgg tggctccaac atgcatctca tcctcaagca
       61 cgtgattccg aacgtgctcc cgttgagctt cctctacatg atgttcacgg tcaccgaggc
      121 tgtcagtctg gaggccaccc tctccttctt cggtctcatc gaggccccga tgacctgggg
      181 catcatgttg agcacagccc agacgcaggg ctacctccga agcggtctcg agttctggtg
      241 gctgctcatt ccggccggcc tcgccgtctc gctccttgcc gcagggttct tcttcgccgg
      301 ccgggcgatg gacgaagtca tcaacccgag gctgagggct caatgacgga caccagattg
      361 aacacagccg agggcaatct ggagaccccc gtgccgaacg gggaggtgcc gatcctcgag
      421 gtcaagaacc tcgtcatgaa ctacgagatg aagtcgggga tggtctccgc cgtcgaggga
      481 gtttcgttca agctccccaa aggcaagtcg ttgggcttgg tgggtgagtc gggctgtgga
      541 aagacctctg tcgccctcac gctgatgcag ctgaccgcca gcaacgcccg ggtgctcgac
      601 ggagagatca aactcgatgg cgagaacctg ctcgagctca gcgacgagga gatgcgcaaa
      661 cgccgctgga acgacgtgtc gatggtgttc cagggcgcga tgaactcgtg gaacccggtc
      721 tacacagtcg gcgagcagat ccgggagacc atggacatcc attgggatcc gaagccgacg
      781 accgcccagg cgcgggcgaa gatcgaggag ctcttcaccc tggtcgggat caaccccgag
      841 atgatcgatc gctatccaca cgagttctcg ggaggcatga aacagcgcgc ggtgatcgcc
      901 atggcgctcg cttgtgatcc caagctgatc atcgccgacg agccgacgac agctctcgac
      961 gtgatcgttc aggagcagat cctcgaggag ctgaggaaga tccaagacga gctcgggatg
     1021 tcgatcatct acatctccca tgacatcgcc gtcatcgccg agatcaccaa cctcatcggc
     1081 gtcatgtacg cagggaagat cgtcgagctc gggcccacga ccgagatctt cgccaaccca
     1141 cgccaccctt acgcgtggct gatcctctcc tcgacgccct ccgtcaccgg cccccgccgt
     1201 gttctggcgc cgctcgaagg cgaaccgccg aacctgctcg acccgccgag tgggtgcagg
     1261 ttccatcccc gctgtccctt cgcgacccag aaatgcgccg atgaggagcc acctctcgcc
     1321 gacatcgagc cgggacacgc cgttgcctgc tggcatcacg accaggtccc ggcaacgctg
     1381 gcaaaggtgg aggcatgacc accgaccaga gcaccaccac cgagcgcacc gggcccaaac
     1441 ccaacggaga ggtgcgggtg aaggtggaca acctccgcaa gctgtttccc atctctcgtg
     1501 ggttgttccg cgcaccgacg gacttcgtgc atgcggtgga cggcgtcaac ttccagatca
     1561 gggcgggcga gtccctgggg ctggtggggg agtccggttg tggtaagacg accaccggtc
     1621 ggatgctcgt caagctggag gatgcaacag acggatcgat actgattcgc gactcggaaa
     1681 gcgacgagct ggtgaatgtg acccacatca actcgaaggc cgatcagaag gtattccgca
     1741 ggaatgtcca gatgatcttc caggacccct acgagtcgat gaatccccgg cggacgatct
     1801 tcgacatcgt cgccgagccc cttaccgtcc agaatctggg caccacctcc gagcgggagg
     1861 agcgggttgt cgacctgttg agactggttg ggctcactcc acccgagcca ttcctctttc
     1921 gctatcccca cgagctgtcc ggcgggcagc gtcagcgggt tgccatcgcc agggcactcg
     1981 tcatggaccc caccttcgtg gtggccgacg agccgacatc catgctcgat gtctctatcc
     2041 ggatatcgat catgcgtctc atgctcgagc tcgccgagaa gctgggcgtc acttacctct
     2101 acatcactca cgacctcgct gtcgcccgct acatgtgcga ccggatcgct gtgatgtacc
     2161 tgggaaagat cgtggagatg ggccccaccg aggatctcct gtccaacccg atgcatcctt
     2221 acacccgggc gttgctctct gctgtccccg tgcccgaccc gtcgtatcgc cggccaccgg
     2281 tcgagatcga gggcggagtg gccagggcca tcaacccctc tgccgagtgt cgtttcatcg
     2341 cccggtgccc tttcgcaacg gacatatgtg ccaccgaaca tccaccactc gacgaccgcg
     2401 gcgacgagca ctacgtcgcc tgctatctcg tcgatgccca aggaaactgg accggcccca
     2461 agcgggccga gctcgacctg cgttcgtgaa ggtgcctgtg aggggggtcg tccccccctc
     2521 cggccctacg ggcctacccc ccgccggcct tcggccggtt gagccacaac gcttggaatg
     2581 cagggatgcc c
//
LOCUS       DASUTA010000091         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1068060, whole genome shotgun sequence.
ACCESSION   DASUTA010000091 DASUTA010000000
VERSION     DASUTA010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068060"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFZ80_01795"
     CDS             <1..252
                     /locus_tag="VFZ80_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286194.1"
                     /translation="IGELVEGEVSLATASGALDIGIARGTSAWVDARTQFGQVRNTLE
                     PTDGPVDSGAKVEIRARTSFGDVSIHRSSPKPRDQSGES"
     gene            249..1208
                     /locus_tag="VFZ80_01800"
     CDS             249..1208
                     /locus_tag="VFZ80_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018932457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6286195.1"
                     /translation="MSNDTRQPAIAVEGLQKKYGDNVVLDGVDLKVQPGSVYALLGPN
                     GSGKTTIVNILTTLIRADGGSARVAGFDVASDPARVREQISVTGQFAAVDYVLTAREN
                     LVLMGELRHIADPAGTAAELLDRFDLNDAADRRLLTFSGGMRRRLDIAMSLVGDAAVV
                     FFDEPTTGLDPKGRVDMWSTIEGLAGGGTTVFLTTQYLEEADRLADEIAILHGGRIVA
                     HGPPDRLKTKLPAGIVELEFAEQEQLAAVQIALDGHHNVSRMDSKLVVATTGSVAEMA
                     DMFVRLRDTGIEPSRLTKQTPTLDDVFFKILEEEMEGSHASSE"
     gene            1219..1968
                     /locus_tag="VFZ80_01805"
     CDS             1219..1968
                     /locus_tag="VFZ80_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6286196.1"
                     /translation="MTRRSLRHTTRSLDTIITVVLTPIALLLLFVYVFGDALGTQTGQ
                     IEYVDFIMPGVIIMTGGSGIAYAALRLSMDLQKGIIGRFRTMPVAPSSVLSGQAFSST
                     LSSLFSAGLVLLVGFFVGFRTAAGVDAWLAFIGLLALFTLATTWMAIFFGLLAKTPEG
                     AGAFAYILLLLVFLSPSFVPTDSMTPLLRGFAENQPITPIVETMRSLLTQGTPGDEAW
                     AAVAWSLGILVVFYALALAMYRRREPIPVGQ"
     gene            complement(1993..2077)
                     /locus_tag="VFZ80_01810"
     tRNA            complement(1993..2077)
                     /locus_tag="VFZ80_01810"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2042..2044),aa:Leu,seq:caa)
BASE COUNT          380 a          653 c          695 g          399 t
ORIGIN      
        1 atcggcgagc tggtcgaggg ggaggtgagt ctggccaccg cgtctggagc tctcgatatc
       61 gggattgcca ggggcacttc ggcctgggtc gatgcgcgca cccagttcgg tcaggtgagg
      121 aacaccctcg agccgaccga cggtccggtc gactctgggg ccaaggtcga gattcgagca
      181 cggacgtctt tcggcgacgt gagcatccat cgctcgtcgc ccaaaccgcg agatcaatca
      241 ggagaatcat gagcaacgac acgaggcagc ccgccatcgc tgtggaaggg ctgcagaaga
      301 aatatgggga caacgtcgtc ctcgatggcg tcgacctgaa ggtccaaccc ggttcggtgt
      361 acgccctgct cggcccgaac ggctccggca agaccaccat cgtcaacatc ctgaccactc
      421 tgatcagggc ggatggggga tcggcgagag tcgccgggtt cgatgtcgcc agcgacccgg
      481 ccagggttcg cgagcagatc agtgtgaccg ggcaattcgc cgcggtggac tacgtgctca
      541 cagctcgtga gaacctggtc ctgatgggcg aactgcgtca cattgccgat ccagccggga
      601 ccgcggcaga gctgctcgac aggttcgacc tgaacgacgc ggccgaccgt cgcctcctca
      661 ccttctcggg agggatgcga cgccggctcg acatcgccat gagcctcgtc ggtgacgccg
      721 ctgtcgtctt cttcgatgag cccactacgg gactcgaccc caaaggtcgc gtcgacatgt
      781 ggtcgaccat cgagggattg gccggaggcg ggaccaccgt gttcctgacc actcagtatt
      841 tggaagaggc cgaccggctc gccgacgaga tcgccatcct ccacggcggg cgaatcgtgg
      901 cgcatgggcc gccggatcgg ctcaagacga agctgcctgc ggggatcgtc gagctcgagt
      961 tcgccgagca agagcagttg gctgcggtgc agatagctct cgacggccat cacaacgtga
     1021 gccggatgga ttcgaaactg gtggtcgcca ccaccggctc ggtggccgag atggcggaca
     1081 tgttcgtcag gctgagggac accggaatcg agccgtcacg actcaccaag cagaccccaa
     1141 cgctggacga cgtgttcttc aagatcctgg aagaagaaat ggagggctca catgcgagct
     1201 ctgagtgaca tgtggacgat gaccaggcgg agcctgcgtc acacgacacg cagtctcgac
     1261 acgatcatca ccgtcgtgct gactccgata gcgctgttgc tgctgttcgt gtacgtcttc
     1321 ggtgacgccc tcgggaccca aaccgggcag atcgagtacg tcgacttcat catgcccgga
     1381 gtcatcatca tgacgggtgg cagcggcatt gcctacgcgg cgttgcgact gagcatggac
     1441 ctccagaagg gcatcatcgg cagattccgc accatgccgg tggctccgtc gtccgtcctg
     1501 agcgggcagg ccttctcgtc gacgttgtcg agcctgttct cagccggcct ggtgctcttg
     1561 gtgggcttct tcgtcggttt ccggaccgcc gctggtgtcg acgcgtggct ggcgttcatc
     1621 ggtctcctgg cgctgttcac actcgccacg acctggatgg ccatcttctt cggactgctg
     1681 gcgaagaccc cggagggggc gggtgcgttc gcatacatcc tgctgctgct ggtgtttctc
     1741 agcccgtcgt tcgtccccac cgattcaatg acgccgctat tgcgtgggtt tgccgagaac
     1801 cagccgatta ctccgatagt cgagaccatg cgatcgctgc tgacccaggg cacaccgggt
     1861 gatgaggcct gggctgcggt cgcatggagc ctcgggatcc tggtcgtgtt ctacgcgctc
     1921 gccttggcga tgtatcgacg gagggaaccg atcccggtag ggcagtagag gggtcggcgt
     1981 ctgggagctg gttggtgccg gaggtgggat tcgaacccac acgcccgtag ggcacggcat
     2041 tttgagtgcc gcgagtctgc cgttcctcca ctccggctcc ttccgcagaa cgatagacct
     2101 gcagccagcg ataatcccac ggtgaac
//
LOCUS       DASUTA010000092         2740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1715123, whole genome shotgun sequence.
ACCESSION   DASUTA010000092 DASUTA010000000
VERSION     DASUTA010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715123"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VFZ80_01815"
     CDS             complement(<1..959)
                     /locus_tag="VFZ80_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007532821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6286197.1"
                     /translation="MAIGVAAVVVAALIGSVVGAAGLDATSVVKALLDAMPGVSMESG
                     LTQAEHAILFDIRLPRIALGALVGAVLAVSGAAYQGVFRNPLADPYLLGVAAGAGLGA
                     TLGFALTGRSGWIAPLAFAGGLGAVAITYVLGRSVGGRSPTSLILAGVAVAAFATAVQ
                     TYILQQNVETLREVYSWILGRLTTVGWGEVIELLPYAVGASLILVVARRLLDVLALGD
                     DEARALGVNVSRVRAVVVITATLATAAAVSVSGLIGFVGIIVPHTIRLLFGWSYRVIV
                     PLSILFGAAFLMLADVAARTLIAPAELPIGVVTAFFGAPFFLLV"
     gene            complement(1013..1999)
                     /locus_tag="VFZ80_01820"
     CDS             complement(1013..1999)
                     /locus_tag="VFZ80_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6286198.1"
                     /translation="MKRSPVLLALVLAVAACGTDGTSSATTAIGDDPTTTKLTTTTVA
                     ITTTEAGSANFPATIEAENGEVTIEEMPSSIVSLSSTATEMLFAIGAGSQVVAVDDQS
                     NHPAGAPVTDLSGFTPNVEAILSYQPDLVVIGYDPGDLVASLDAADVPVINYSAAVTI
                     DDVYRQIEALGAATGHAEEAAAVNDSISDDLARIANEAPDAEGVTYYHELDNTLYTAT
                     STTFFGQIYGLFGLENIADAADEDGAAFGYPQLSSEYVVSADPDIIFLADTLYGESSE
                     TVAARPGWDVLAAVQNGNIVELDSDIASRWGPRIVDFAEAVSEAIDSYVSQG"
     regulatory      complement(2082..2239)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2309..>2740)
                     /locus_tag="VFZ80_01825"
     CDS             complement(2309..>2740)
                     /locus_tag="VFZ80_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX6286199.1"
                     /translation="GALVDRDGQVVGINVSIFSTSGANDGVGFAVPSDIAVTYADGII
                     SGEPIETAFLGVQADDVNAEGQAGAEITVVVDDSGADTAGLQVGDVVVGFAGVPVRGF
                     GDLVAQVRAHQPGESVEVLIIRDGEEQSVQVTLGVNTEDLS"
BASE COUNT          527 a          871 c          893 g          449 t
ORIGIN      
        1 accaggagga agaagggagc accgaaaaag gcggtcacca ccccaatggg cagctctgca
       61 ggcgctatga gggtccgggc tgccacatcg gcgagcatca ggaaggcggc tccgaagaga
      121 atcgagaggg gaactatcac ccgatagctc caaccgaaga gcagccgaat cgtgtgaggc
      181 acgatgatcc ccacgaaacc gatcagccca ctgaccgaga cggcggcggc ggtggccaga
      241 gtggcggtga tgacgactac ggctctcacc cggctcacat tgaccccgag cgcccgggcc
      301 tcgtcgtcac ccagagcgag cacgtcgagg agacgacgcg ccacgaccag gatcaacgaa
      361 gcgccgactg catagggcaa gagctcgatg acctcgcccc atcccacagt cgtaagtcgt
      421 ccgagtatcc acgagtagac ctcgcgcaag gtctccacgt tctgctggag tatgtaggtc
      481 tgcacggccg tagcgaacgc ggcgaccgca accccggcaa ggatcaggga cgtcggggac
      541 cttccaccga cggagcgtcc gaggacatag gtaatggcga ccgcaccaag accaccggcg
      601 aacgccagcg gggcgatcca tcccgatcgg ccggtgaggg cgaacccgag tgtggctccg
      661 aggccggcac ctgctgcgac cccgagcagg tacgggtcgg cgagagggtt gcggaaaacg
      721 ccctgatagg cggctccgga gacggcgagg accgccccaa ccagggcgcc gagggcgatc
      781 cgggggagcc gaatgtcaaa gaggatcgca tgctcggcct gagtcagtcc cgattccatc
      841 gacactccgg gcatagcgtc gaggagagcc ttcacaaccg atgtcgcgtc gagaccggcc
      901 gcccccacca ccgacccgat gagggcagcc accacaacgg cggcgacacc gatcgctatc
      961 gccacccggc cggtcttggc cggacgagct tcgaccggtg cgatcgccgc actcaaccct
     1021 ggctcacgta cgagtcgatg gcctctgaga cggcttctgc gaagtcgacg attcgggggc
     1081 cccaccggga ggcgatgtcg gagtcgagct cgacgatgtt cccgttctgc accgcggcca
     1141 agacgtccca cccagggcgg gcggcgacgg tctccgagct ctcgccgtag agggtgtcgg
     1201 cgaggaagat gatgtctgga tccgcggaga ccacgtattc gctggagagt tgcggataac
     1261 cgaaggccgc gccgtcttcg tctgctgcgt cggcgatgtt ctccagcccg aacaatccgt
     1321 agatctgtcc gaagaaggtc gtcgatgtcg ccgtgtacaa ggtgttgtcc agttcgtggt
     1381 agtaggtcac cccttcggca tcgggggcct cattcgcaat ccgcgccagg tcgtctgata
     1441 tcgagtcgtt gacagcggcc gcctcctcag cgtgaccggt tgcagctccg agggcttcga
     1501 tctggcgata gacatcgtcg atggtcaccg ccgcgctgta gttgatcaca gggacatccg
     1561 cggcatccag cgacgccacc agatccccgg ggtcgtatcc gatgaccacc aggtccggct
     1621 gataggagag aatcgcctcc acgttcgggg tgaaccccga gaggtcggtc accggggccc
     1681 cggctgggtg gttggactgg tcgtccacag cgacgacctg ggagccggcg ccgatggcga
     1741 acagcatctc ggtcgcagtc gacgagagcg acacgatcga ggatggcatc tcttcgatcg
     1801 tcacctcgcc gttctcggct tcgattgtgg cagggaagtt ggcagagccg gcttcggtgg
     1861 tggtgatcgc cacggtggtc gtggtcagtt tcgtggtggt cggatcgtca ccgatcgcag
     1921 tcgtggcaga acttgtgccg tctgtgccac aggctgcgac ggcgagaacg agcgccagga
     1981 gtaccgggct ccgcttcata tgagaacatc ctccgtcggt cggaggatga agaggtggtg
     2041 ggcttccctt gggcccacgc ttccgtcttc atccgagact ttcacgtggc cccgagacag
     2101 gcgacctggc tcgtgcattc tcttgggtga cggcgagaat cgtcgttggc caagggacgc
     2161 cttcacagtt gcgggacagc gccgggttcc aaccggcttc gctgttcgcg aggcactcac
     2221 ctattgcggt gagcggaatc agcttagtgg tttgagcgtg tgcagatacc ggcccctggc
     2281 cacgctattt gcacacgctc gaagacgctc agctcagatc ctcggtgttg acgccgaggg
     2341 tcacctggac cgactgctcc tcaccatcgc ggattatcaa gacctcgaca ctctcgcctg
     2401 gctgatgggc gcgcacctga gcaaccaggt caccgaaccc tcgaacgggg acgcctgcga
     2461 aacccacgac cacgtcaccg acctggagac cggcggtgtc ggccccggag tcgtcgacga
     2521 ccacggtgat ctcagcgccg gcttgaccct cggcgttgac atcgtcggcc tggaccccga
     2581 ggaacgccgt ctcgatcggc tctccggaga tgatgccgtc ggcataggtg acggcaatgt
     2641 cagaggggac ggcgaaccca actccgtcat tggcaccgga agtgctgaag atggagacgt
     2701 tgatccccac gacctggccg tcccgatcta ccaatgcccc
//
LOCUS       DASUTA010000093         2552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_161953, whole genome shotgun sequence.
ACCESSION   DASUTA010000093 DASUTA010000000
VERSION     DASUTA010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161953"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            465..542
                     /locus_tag="VFZ80_01830"
     tRNA            465..542
                     /locus_tag="VFZ80_01830"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:500..502,aa:Asp,seq:gtc)
     gene            569..644
                     /locus_tag="VFZ80_01835"
     tRNA            569..644
                     /locus_tag="VFZ80_01835"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:602..604,aa:Phe,seq:gaa)
     gene            complement(915..1718)
                     /locus_tag="VFZ80_01840"
     CDS             complement(915..1718)
                     /locus_tag="VFZ80_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286200.1"
                     /translation="MEDDSPGERIVTLHSSPDEGLGLSSRPAINENQRTSSMYPTATT
                     PATRAELAPVVAKSLTRSSRLATPPRYPRFLRVRSPSSITDKTSKSVAQATGVPEPRI
                     TALFEHITSRDLTDDLIDLEAIDIADGLEVRFKKPENNRFTDIENLAHGQKCTAILII
                     ALAEGDEPLLIDQPEDALHAPWIEEYLVDRLRELRGSRQYVFATRSPGLVVSAYAEML
                     ITLVSQADSGHVEASGSLERHELNELALYHLEGGSTPFARRARKLSDSL"
     gene            1946..2164
                     /locus_tag="VFZ80_01845"
     CDS             1946..2164
                     /locus_tag="VFZ80_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286201.1"
                     /translation="MRWVKWGLAVIVGGPLVIQLVPYGRVYANPAVVAEPSWDSPQTR
                     ELAVRACFDCHNTRRCGPGIRISLPCPG"
     gene            2152..2412
                     /locus_tag="VFZ80_01850"
     CDS             2152..2412
                     /locus_tag="VFZ80_01850"
                     /inference="COORDINATES: protein motif:HMM:NF025734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HEX6286202.1"
                     /translation="MSWLMQRDVDEGREELNYSEWSRRQESGESAETVRERSMPPRSY
                     LLPHPDARLTDADLAALANDMTATFGDEGEGEGEEGEGEENH"
     gene            2412..>2552
                     /locus_tag="VFZ80_01855"
     CDS             2412..>2552
                     /locus_tag="VFZ80_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013978674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286203.1"
                     /translation="MEDSASPAVRCSALTKDYGSGHGVFDLDLTVEEGEVFGFIGPNG
                     AGK"
BASE COUNT          481 a          734 c          799 g          538 t
ORIGIN      
        1 ctcgtgcaag cgacgagttg gcccccgtgt gaccttcgac ccaatcgatg tctcctgccc
       61 tcttcgtagg ttggtagtac cgacggatgc cgacctggaa agggctgaca gaagtgagcc
      121 aagaccgaac gatgactggc gtgacatcat tgtcggattc ggaatcatcc ttggcgctgc
      181 gatcggcgcc accaacggac tgattctgac gggcggggag gtcttgtcct cggcggcgct
      241 ttctgcgccg cgggagctgt cgtgttcggc gcaatagccc gaagtcggta cgagagccgc
      301 aaatagcaaa tcaatctgcc tcaacgaact cctggatcgc ccgctgggct ccggatgatg
      361 atgggtggat cgtgttgtgt ctctgtggct cgacccccgc cgatgttgag caggcaccgg
      421 ggaccgatat aattctgcga cgcccgaact ccgggccagc tccagggcgc gtagaacagt
      481 ctggagtgtt cgccggcctg tcaagccgga ggtcgcgggt tcaaatcccg tcgtgcccgc
      541 cacccaattc gtcagagttg aggtgcaagg tcaggtagct cagtcggtag agcgccggtc
      601 tgaaaaaccg gaggtcgacg gatcgacgcc gtccctgacc accaatccct accagggaaa
      661 tcaccctaaa cgtcctggta gacaccaaga agtggcgacg gggcacttct ctcctcattt
      721 ggcatggtct cgccgacacg tgcctcatct cgcatgtgtc gcggcacaaa tgcggcacag
      781 gaggcatatt cacgatcacg atttacgtga gtgatctctc cagcgccttg cgaaacgact
      841 cgatggcgat ctcgagatac gacttcgaag aatgaccaga tggccagccg agccaccaga
      901 acgcggccat tctgctagag cgagtccgag agtttccgag cgcgccgagc gaagggcgtc
      961 gatccaccct ctaggtgata cagagcgagt tcgttgagtt cgtgtctttc aagcgaacct
     1021 gaggcttcga cgtgtccact atccgcttgt gagacaaggg tgatgagcat ctcggcgtag
     1081 gcactcacga ccaatcctgg acttcttgtg gcaaacacat actgccgtga tcctctcagc
     1141 tcacggagtc ggtcgactag gtactcttcg atccagggtg catgcaaggc gtcttctggc
     1201 tgatcaatga ggaggggctc atcgccctca gctagagcga tgatgagaat ggccgtgcac
     1261 ttctggccgt gtgcgaggtt ctcaatatcg gtgaatctgt tgttctcggg cttcttaaat
     1321 cgcacctcca ggccgtcggc aatgtcgatc gcttcgaggt caatgagatc atctgtcagg
     1381 tctcgactag tgatgtgttc gaacaaggct gtaattctcg gttctggaac tcccgttgcc
     1441 tgtgcaacgg atttgctcgt cttgtcggtg atgctgctcg gcgaacgtac cctaagaaac
     1501 cttgggtatc ttggcggggt cgccaacctg ctgctcctgg tgagcgactt tgccaccacc
     1561 ggggccagtt ccgctctcgt cgccggcgtg gttgccgttg ggtacatact gctcgtcctc
     1621 tggttctcgt tgatggccgg tcggctgctc agccccagac cctcatccgg agacgaatgg
     1681 agggtgacga ttcgctcgcc cggagagtcg tcttccacgg cttccgaccc cgaccgcgac
     1741 gacacctcgc cgccatctgc ctcatcagaa caggcgaggt cgtgtatgtc atgctctact
     1801 ttgccggtct cggcgagatg atcggggtgg aatcgccccg cgccgctgga attggggcag
     1861 aacgtctatg ccgagagccg ggtctcgtcg cgagcgggta cttcgcctct ggccaccggc
     1921 gtcgttccgg gtaagaatcg tgatcatgcg ttgggtgaaa tggggtctcg ccgtcattgt
     1981 cggtgggcca ttggtgatcc agttggtgcc ctatggaagg gtctatgcca accccgcggt
     2041 ggtggcggag ccgtcgtggg attcacctca gacccgtgag ttggcggtcc gtgcctgttt
     2101 cgactgccac aacacgagac ggtgtggccc tggtattcga atatcgctcc catgtcctgg
     2161 ctgatgcaac gggatgtcga cgaaggccgg gaggagctca actactcgga gtggagccga
     2221 cgccaggaga gtggggagtc ggccgagact gtgcgggagc gttcgatgcc accacgctcc
     2281 tacctgctgc cccatcccga tgcccgtctc accgacgccg acctggccgc gctggcgaac
     2341 gatatgacag ccactttcgg tgacgaaggc gaaggcgaag gcgaggaggg ggagggggag
     2401 gagaaccatt gatggaagac tcggcgtcgc ctgcggtccg ttgttccgct ctgaccaaag
     2461 actatgggag tggtcacggt gtcttcgacc tcgacctgac cgtcgaagag ggggaagtgt
     2521 tcggattcat cggacccaac ggggccggaa ag
//
LOCUS       DASUTA010000094         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_420437, whole genome shotgun sequence.
ACCESSION   DASUTA010000094 DASUTA010000000
VERSION     DASUTA010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420437"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VFZ80_01860"
     CDS             <1..230
                     /locus_tag="VFZ80_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013937537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate kinase"
                     /protein_id="HEX6286204.1"
                     /translation="ASDRLRNFVDKAAQATLVGDVFDDAATGQGLLNYFLRGINCGAI
                     TEDEATTRTGLTVDELRTRSFLRILEGRRGS"
     gene            complement(290..1201)
                     /locus_tag="VFZ80_01865"
     CDS             complement(290..1201)
                     /locus_tag="VFZ80_01865"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX6286205.1"
                     /translation="MLVGMADLDVAVRRVAREQHFLITRSQVLDLGGSPSSIDARIKS
                     GAWLRRQCGVVQVDQRPMSWRTRLMAAVLAAGDGALVSHRAAFVLWGLDGIATEMVEL
                     TVPYTHGPVPRGVVLHRSRRPEEGREVDGIPITSVERSLLDCSARFDRRLIGKGLDSA
                     LRKNLTTMDDVYDLLVAKGGRGVKGTKTLRWVLRERIHDTSTDSGSEYDLLYHMQMAF
                     LPRPELHYPVFVDGRRVPDFYWPHVGKAVEVDGVDAHSSADKLDDDLKRQNALMDMGI
                     ELRRFSAREVRRNPKGVVAQISQFLAG"
     gene            complement(1294..2106)
                     /gene="pucL"
                     /locus_tag="VFZ80_01870"
     CDS             complement(1294..2106)
                     /gene="pucL"
                     /locus_tag="VFZ80_01870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004846 - urate oxidase activity [Evidence
                     IEA];
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HEX6286206.1"
                     /translation="MALGSNSWGKSDVRVSKVFRGDGEDDFLDLNVQILLQGDVGAAH
                     TEGDNSNVVPTDTMRNTVFGLAQDHLSRDLERFGELLCEHFLAKTGVEQAEVTILEHL
                     WARETSNGFIGGGSERRRARVARGAETSTSAGIEGLVVLKTGGSAFEGFPRDEYTVLP
                     ETADRVLATSITAGWDYGTVPEDTSATWALVRSTLVERFFDDWSASVQHQGYLMGEAV
                     LAAVPEVTEISFRLPNQHHLPFDLTRFGLDWEGTVFHPVTAPYGDIHLTVTR"
     gene            complement(2108..2422)
                     /gene="uraH"
                     /locus_tag="VFZ80_01875"
     CDS             complement(2108..2422)
                     /gene="uraH"
                     /locus_tag="VFZ80_01875"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEX6286207.1"
                     /translation="MTTLSTHVLDTVTGEPARGMRVHVSRVGDNGWERIAEFATDENG
                     RVAGFGELGPGRHRLGFETGEYGNDFYPFVAVVFEIDDQRPHYHVPLLLSPFGYTTYR
                     GS"
     gene            complement(2419..>2868)
                     /gene="uraD"
                     /locus_tag="VFZ80_01880"
     CDS             complement(2419..>2868)
                     /gene="uraD"
                     /locus_tag="VFZ80_01880"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF010372.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEX6286208.1"
                     /translation="ATGFFHSACASRAWVRRMIDERPFASVDELLTTAEYTFGHLGEG
                     DWLEAFSGHPRIGERGDATANREQAGTATASRETIRDLIDVNRRYEDKFGFTYIVYAT
                     GKTADVMLGIARDRLENDEEQEIALAAGEQRKITANRLRRMLCQEVK"
BASE COUNT          520 a          962 c          886 g          500 t
ORIGIN      
        1 cggcctccga ccggctgcgc aacttcgtcg acaaggccgc gcaggccacg ctcgttggtg
       61 acgtgttcga cgacgcggcg accggccagg gcctcttgaa ctacttcctg cgcggcatca
      121 actgcggcgc catcaccgaa gacgaagcca ccacccgaac cgggcttact gtcgacgagt
      181 tgagaacccg ctccttcctc cgcatcctgg agggccgccg cgggtcctga gaaacccttc
      241 tgtgagtaga acagtccaga tcctggactg ttctactcac agaacggagt cagcctgcca
      301 ggaattggct gatttgggcg acgacgccct tcgggttgcg gcggacctcg cgtgcggaga
      361 agcgacgcaa ttcgatgccc atgtccatga gggcgttctg ccgcttcaga tcgtcatcga
      421 gtttgtcggc agaagagtgg gcatccaccc cgtcgacttc gacggccttg ccgacatggg
      481 gccagtagaa gtcagggaca cgccgcccgt cgacaaacac cggatagtgg agctcgggtc
      541 gggggagaaa cgccatctgc atgtgataga ggaggtcgta ctcggagccg ctgtcggtgg
      601 acgtgtcgtg aatccgctcc cgcagaaccc agcgcagagt cttcgtgccc ttgacaccgc
      661 gacccccttt ggccaccagg aggtcgtaga catcgtccat cgtggtcagg ttcttcctca
      721 aggcggagtc cagccccttc ccgatcagtc gccgatcgaa cctggcggag cagtcgagca
      781 agcttcgctc gacggaggtg atcgggatcc catccacctc acgcccttcc tccggtctgc
      841 gtgagcgatg aaggaccacg cccctgggaa ccggcccgtg ggtgtacgga acggtcagtt
      901 cgaccatctc ggtagcgatc ccgtcgagtc cccacagcac gaacgccgcc ctatgggaga
      961 cgagcgctcc atctcctgcg gcgaggacgg ccgccatcag ccgcgttcgc caactcatgg
     1021 gacgctggtc gacctgcacc actccgcatt gacgcctcag ccatgcaccc gacttgatgc
     1081 gggcgtcgat cgacgatgga gaaccaccga ggtcgagcac ctgcgagcgt gtgatcagga
     1141 aatgctgctc tctcgcgacg cgtcgaacgg caacgtcgag atcggccatg ccaacgagca
     1201 ttgccacgac gtggccctcg ctgcaagccc cttccgcaac tgtgagtaga acggtccaga
     1261 ttctggaccg ttctactcac agaacggtct tcttcaacgg gtgacggtga ggtggatgtc
     1321 gccgtagggg gcggtgaccg ggtggaagac ggtgccctcc cagtcgagac cgaagcgggt
     1381 gaggtcgaaa ggcagatggt gttggttggg gagacggaag ctgatctcgg tgacctcggg
     1441 cacggcggcg aggaccgcct cccccatgag gtatccctga tgttggaccg acgccgacca
     1501 gtcgtcgaag aaccgctcga cgagggtgga gcggaccagg gcccatgtgg ccgatgtgtc
     1561 ctcggggacg gtcccgtagt cccagcccgc ggtgatcgag gtggccagca cacggtccgc
     1621 ggtctcgggg aggaccgtgt actcgtccct ggggaagccc tcgaacgccg acccgcccgt
     1681 cttgaggacg acgaggccct cgatgccggc cgaggtcgac gtctccgcgc cacgcgcgac
     1741 acgggccctc cgccgctccg agcctccacc gatgaaccca ttcgaggtct cccgggccca
     1801 cagatgctcg agaatcgtca cctccgcctg ctccaccccg gtcttggcga gaaagtgctc
     1861 acacaacaac tcaccgaatc tctccaaatc cctgctcaga tgatcctggg cgagcccgaa
     1921 gacagtgttc cgcatcgtgt ccgtggggac gacgttcgag ttgtccccct cggtgtgggc
     1981 tgccccgaca tcgccctgga ggaggatttg gacgttcagg tcgaggaagt cgtcttcacc
     2041 gtcaccgcgg aagaccttcg acacccggac gtcgctcttc ccccaactgt tggagccgag
     2101 tgccacgtca actcccccga taggtcgtgt agccaaacgg gctcaacagc agaggcacgt
     2161 ggtagtgggg ccgctggtcg tcgatctcga acacgacggc gacgaacggg tagaaatcgt
     2221 tgccgtactc accggtctcg aagccgagac gatgacgccc cgggcccagt tccccgaagc
     2281 ctgcgaccct cccattctca tctgtggcga actccgcgat ccgctcccaa ccgttgtcac
     2341 cgacgcggga cacatggacc ctcatccccc tggcgggctc tcccgtgacc gtgtcgagta
     2401 cgtgggtgct gagtgttgtc acttgacctc ctggcacaac atacgacgaa gacggttcgc
     2461 cgtgatcttc cgctgctcgc cggcggcgag cgcgatctcc tgctcctcgt cgttctccag
     2521 ccgatcccgg gcgatgccga gcatgacgtc ggctgtcttc cccgtggcat agacgatgta
     2581 ggtgaagccg aacttgtcct cgtagcgccg gttgacgtcg atcagatccc ggatcgtctc
     2641 cctcgacgcc gtggcggtcc ccgcctgctc ccggttggcc gtcgcgtcac cccgctcgcc
     2701 gatgcgagga tgaccggaaa aggcctccag ccaatcaccc tcgccgagat ggccgaaggt
     2761 gtactccgcg gtggtcagca gctcgtccac cgaggcgaac ggacgctcgt cgatcatcct
     2821 ccgcacccag gcacgcgaag cgcaagcgga gtggaagaac ccggtcgc
//
LOCUS       DASUTA010000095         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1069268, whole genome shotgun sequence.
ACCESSION   DASUTA010000095 DASUTA010000000
VERSION     DASUTA010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069268"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VFZ80_01885"
     CDS             complement(<1..647)
                     /locus_tag="VFZ80_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018840463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX6286209.1"
                     /translation="MSIFQNLLRAGEGKTLKEFQGIANAVNELEPEYEALSDEDLKAK
                     TPEFKNRIANGAEVDDLQIEAFATVREAAKRVLHQRHYDVQIVGAAALHRGMVAEMKT
                     GEGKTLVSTMPAYLNALTGKGVHMVTVNDYLAGRDAEWMGHVHRFLGLSVGLILNNMP
                     TADRHPAYRSDITYGTNNEFGFDYLRDNMAMSADNRVQRGHVYAIVDEVDSILID"
     gene            complement(720..1370)
                     /locus_tag="VFZ80_01890"
     CDS             complement(720..1370)
                     /locus_tag="VFZ80_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6286210.1"
                     /translation="MATVLIVDDAELFREALRAAFQQEGFDVVGVAADAMSGIDLARE
                     HEPDLVMLDLLMPGMSGLEVVSTILKASVRSRVVLLTASESAEDLLAAVKAGASGYLT
                     KDTPLPRLASDMRDVLHGGAAISPAMGGKLFSALRDLLKHNGVTSLRRPELTGREIEI
                     LGLVGAGKTSKEIADELFISENTVRNHVRNILDKLGMKSRFEAVNWAHREGLIEIR"
     gene            complement(1374..1880)
                     /locus_tag="VFZ80_01895"
     CDS             complement(1374..1880)
                     /locus_tag="VFZ80_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286211.1"
                     /translation="MTGGLRVIASFEHDGVARRLVHLLKYRGVTAYAGMVASILATRL
                     PALPLVPIPRALSRTMTYGIDPAFVIAREVSARNGQPVIRALGAPIHTRRRAGGDHHR
                     PPPTFGLRKSLREPVILVDDVVTTGGTILSATNALGADVVAIAAAANAVLEVTSLRRP
                     AQINQGGG"
BASE COUNT          378 a          619 c          635 g          407 t
ORIGIN      
        1 tcgtcgatca agatcgagtc cacctcgtcg acgatggcgt agacatgccc acgctggacc
       61 ctgttgtccg ccgacatcgc catgttgtcg cgcaaatagt cgaagccgaa ctcgttgttg
      121 gtcccgtagg tgatatctga ccgatatgcg gggtgtcggt cagccgtcgg catgttgttc
      181 aggatcagac cgacactcag cccgagaaat ctgtgaacgt ggcccatcca ctccgcatct
      241 cgtccggcca ggtagtcgtt gaccgtgacc atgtggacac ctttgccggt gagagcattg
      301 agataggcgg gcatcgtcga gacgagcgtt tttccttcac cggtcttcat ctcggcgacc
      361 atgccgcggt gaagagcagc ggcaccgact atctggacgt cgtagtgacg ctgatggagg
      421 acgcgtttgg ccgcctctct gacggtggcg aatgcttcga tttggaggtc gtcgacctcg
      481 gcaccgttgg cgattcgatt cttgaactcg ggggtcttcg ccttcaggtc ttcgtcgctg
      541 agggcttcgt actcaggttc gagctcgttg accgcattgg caatcccctg aaactccttg
      601 agggtcttgc cttccccagc acgaagcagg ttctggaaga tggacatctg aagcctcaat
      661 tgtaccgatc gagccgagtt gacccgattc ttcgccgtgg ccgcggcggt tggatgcccc
      721 taccggatct cgatgagccc ttcgcggtgg gcccagttga ccgcctcgaa acgcgacttc
      781 atcccgagct tgtccaggat gttgcggacg tggttacgga cggtgttctc ggagatgaag
      841 agctcgtcag ctatctcctt ggacgttttc cctgcgccaa ccaggccgag gatctcgatc
      901 tctcgcccgg tgagctcggg gcgacgcagc gatgtgaccc cgttgtgctt gagaaggtcg
      961 cgaagggccg agaacagttt ccctcccatg gccggtgaga tcgcggcgcc gccatggagg
     1021 acgtcgcgca tgtcggaggc cagtctgggc aacggtgtgt ctttggtcag atagccggat
     1081 gccccggcct tgaccgcggc cagcaggtct tcggccgact cggaggcggt cagcagcact
     1141 acacgactcc gaacactcgc cttgaggatc gtgctcacca cctcgagacc ggacatgccc
     1201 ggcatgagga gatcgagcat gaccaagtcg ggctcgtgct cgcgtgcgag gtcgattccg
     1261 gacatggcgt cggctgccac tcccacgacg tcgaaaccct cttgctggaa tgccgctcgc
     1321 agagcttccc tgaatagctc ggcatcatcg acgatcagaa cagtggccaa acctcagccc
     1381 ccaccttggt tgatttgggc aggccggcgc agactagtca cctcgagtac ggcgttggcc
     1441 gccgccgcga ttgcgacgac gtcggccccc aatgcgttcg ttgcggaaag aatcgttcct
     1501 cctgtggtca caacgtcgtc gaccaagatg acgggttccc ttagcgattt ccgcaatcca
     1561 aaggttggcg ggggacgatg gtgatcgccg ccggcacgtc gccgggtgtg tatcggggct
     1621 cccagggccc gtatcaccgg ctgcccgttg cgggcagaca cctcacgggc gattacgaac
     1681 gcaggatcga ttccgtacgt catcgttcga gacaaagcgc ggggtatcgg gaccagaggt
     1741 agcgcaggca aacgcgtcgc caggatcgag gcgaccatgc cggcatacgc cgtcactcct
     1801 cgatacttga gcagatgcac caaacgccgg gccactccat cgtgttcgaa cgaagcaatg
     1861 acacgcagcc cgccagtcaa cagtcgctca ggtgcgggcc gaaggctcgc cagacactga
     1921 gtgcataggc ggctgcccgg gcggtggcag gcgatgcaga gcattcccga ccgtctatcg
     1981 gtggtgagtg acagaaaatc gaaggcgcgt tggcggagtc ctgcacactc ccggtgaac
//
LOCUS       DASUTA010000096         5353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_162381, whole genome shotgun sequence.
ACCESSION   DASUTA010000096 DASUTA010000000
VERSION     DASUTA010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162381"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VFZ80_01900"
     CDS             complement(<1..795)
                     /locus_tag="VFZ80_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HEX6286212.1"
                     /translation="MIDFVLPSLGADMDQAMLVAWLVEPGSVVKYGQIVAELETDKGI
                     IEVECWHDGVVEELLVEPSPTKLAVGTPLARIRLEDEAPTSDRPTRVTSTPSGPSESR
                     PPTAPRPPTPPVRHLAHTLGVDIEHLAGTGEDGSVTREDVRKAARPKGAAVSPAVASD
                     VADRVKASPRARALATKQGVPLEAITPSREDGLIVHADVAVMEIEPEEPEDKVAAMRR
                     AITRSMSRSKREIPHYYLATTIDMSRALTWLEEVNSDLPLAKRILPA"
     gene            complement(792..1790)
                     /locus_tag="VFZ80_01905"
     CDS             complement(792..1790)
                     /locus_tag="VFZ80_01905"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX6286213.1"
                     /translation="MTDVITEPRTMSYREAMREAMSEAMRLDERVFLMGEDVGEYGGC
                     YAVSKGMLQEFGPERIRDTPLSESAFVGAGIGAALGGMRPIVEVMTVNFSLLALDQIV
                     NTAALYLHMSGGQFNVPLVIRMATGAGRQLAAQHSHSLEGWYAHVPGLKVLAPGTIED
                     ARWMLAPALDDPDPVIIFEHAGLYNMEGVLPGGDEPADISSARVRRPGTDVSLITYGG
                     SLPKALEAAGELESSEISAEVLDLRVLRPLDRTAILDSVARTHRVVVIDEGWKTGGIS
                     AEIAALIAENAIWELDAPIARVCSAEVPIPYARHMEQAALPKVDSIVAEARALVGR"
     gene            complement(1787..2758)
                     /gene="pdhA"
                     /locus_tag="VFZ80_01910"
     CDS             complement(1787..2758)
                     /gene="pdhA"
                     /locus_tag="VFZ80_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018768805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX6286214.1"
                     /translation="MSIGEHHLVLLRQMLRIRRFEERCAQLYQQEKIRGFLHLYIGEE
                     AVATGVMQALGPEDAVVATYREHGHALARGMDPRVVMAEMFGKVEGCSRGRGGSMHLF
                     DSSTRFYGGNAIVGGGLPLAVGLALADKMSLSPRVTAVFFGEGAVAEGEFHEAMNLAA
                     LWNLPVLFCCENNLYAMGTALDRSESQTRITLKAASYQMPAWEVDGMDVLAVEKAARA
                     AAESVRSGGGPHFLELRTYRYRAHSMFDPELYRSKDEVESWKHRDPIESFRDRLLADR
                     VLTDDAWSMMEKEVAEEIDVAVEFAEAGSWEAVDDLTRFVYSEVSGT"
     gene            complement(2755..4530)
                     /gene="acsA"
                     /locus_tag="VFZ80_01915"
     CDS             complement(2755..4530)
                     /gene="acsA"
                     /locus_tag="VFZ80_01915"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018768806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX6286215.1"
                     /translation="METQYPAIDKSGPFRTPPNLEDYERARAAFSWSEAERYLDGLPG
                     GKLNIAHEAIDRHLPDHGDHPAIRWLGRKGDRRDYTYGELGEATSRFASGLRRLGVGR
                     GDRVFLLSTRIPELYIAALGALKNGSVASPLFSAFGPEPIQTRMELGSGKVLVTTETL
                     YRRKVAGIRSQLPGLEHVIVIGSEGTDTVAFEDLLKEGDPDTAAEPMDPEEMALLHFT
                     SGTTGTPKGAVHVHGAVLHHYVSALYALDLHPDDIYWCTADPGWVTGMSYGIVAPMGH
                     GVTSIVDEGEFDAERWYRIIEQEKVSVWYTAPTAIRMLMMAGEEVARGFDLSSLRFVA
                     SVGEPLNPEGVVWGQRVFGLPIHDNWWQTETGGIMIANYIGEQIRPGSMGRPLPGIEA
                     AILRRDDKGEVRLSPDGTAQEESIPLTEGELALRPGWPSMFRGYLGMEERYAKSFVGG
                     WYCSGDLAERDEDGFFWFVGRGDDVIKSAGHLIGPFEVESALIEHEAVAEAGVIGKPD
                     PVAGEVVKAFVALNDGYEWSDELRKELIGWGRVKLGASVAPREVELCQDLPHTRSGKI
                     MRRLLKAWELGLPTGDTSTLEGEEQ"
     gene            4670..5134
                     /locus_tag="VFZ80_01920"
     CDS             4670..5134
                     /locus_tag="VFZ80_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006357550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEX6286216.1"
                     /translation="MDTRIIDTKSIGSRKTAVRIAELDVEALAEWLPETFGIVARHLD
                     ETGDPPIGPPFARYRRLGPRRFHVEAGFPVGRAIYGTDEIVGSSLPGGTVAITTHVGP
                     YDGMEATYEALATWLDSRGSVPMGDPWEVYFSEPTDPPSMWRTDIVQPYLKP"
BASE COUNT          986 a         1775 c         1612 g          980 t
ORIGIN      
        1 cgcggggagg atgcgcttgg ccagcggaag atccgagttg acttcttcca accaggtcag
       61 ggcacgactc atatcgatgg tcgtagccag gtagtagtgc gggatctccc gcttggatcg
      121 actcatcgaa cgggtgatgg cccgccgcat ggcagcgacc ttgtcctcgg gctcttcggg
      181 ttcgatttcc atcaccgcca cgtcggcatg cacgatcaga ccgtcctccc gcgacggcgt
      241 gatcgcctcg agaggaactc cttgtttcgt cgccaaggca cgagcacggg gtgatgcctt
      301 caccctgtct gcgacgtcgc tcgcaaccgc gggcgagacg gcggcaccct tgggtcgggc
      361 cgccttcctc acgtcctctc gcgtcacaga cccgtcttcg ccggttccgg ccaagtgctc
      421 gatgtcgact ccaagggtgt gggccagatg gcggactggt ggcgtcggag gtcttggcgc
      481 cgtcggcggc cgactctccg aagggccgga aggagtggat gtgacccggg tcggacggtc
      541 cgatgtcgga gcctcgtcct cgaggcgaat tcgcgccaga ggagtgccga cggccagctt
      601 cgtcgggctt ggctcgacca gcagctcctc caccacgccg tcgtgccagc attcgacctc
      661 gatgattcct ttgtcggtct ctaattcggc gacgatctga ccgtacttca ccaccgagcc
      721 cggctcgacc agccaggcca ccagcatggc ctgatccatg tcagcgccga gtgagggaag
      781 aacgaagtcg atcatcggcc gacaagcgcc ctcgcttcgg cgacgatcga gtcgaccttg
      841 gggagagcag cctgctccat atgacgggcg tacggtattg gtacctcggc cgagcacacc
      901 cgtgcaatgg gggcatcgag ctcccagatg gcattctcgg caatgagagc ggcgatctcc
      961 gccgagatac cgccggtctt ccatccctcg tctatcacca ccacacggtg ggtccgcgcc
     1021 acggaatcga ggatggcagt ccgatccaat ggtcgaagga ctctcagatc caacacttct
     1081 gcggagatct cgctcgactc gagctccccg gcggcttcga gagccttggg gaggctccct
     1141 ccgtaggtga tcaggctcac atctgtgcca ggacgcctca cccttgcgct ggagatgtca
     1201 gccggttcgt ctccgcccgg caggacccct tccatgttgt agagaccggc atgctcgaag
     1261 atgatcaccg ggtcggggtc gtcgagggcg ggagccagca tccatcgggc gtcctcgatg
     1321 gtcccaggtg ccagcacctt gagcccgggc acgtgtgcat accaaccctc gagagagtgg
     1381 gaatgctgtg ctgccagctg acggccggca ccggtggcca tcctgatcac gagtggaacg
     1441 ttgaactgac caccggacat gtggagatag agggcagccg tgttcacgat ctggtcgagg
     1501 gcgaggagag agaagttcac cgtcatgacc tccacgatgg gacgcatccc accgagggcg
     1561 gcgccgatcc ctgcccccac gaaagcggac tctgacaacg gggtgtcacg gatacgctcc
     1621 ggcccgaatt cttgaagcat ccccttggag acggcgtaac agccgccgta ttccccgacg
     1681 tcctcaccca tcaggaacac ccgctcgtcc aagcgcatcg cctcggacat ggcctcccgc
     1741 atcgcctccc gatatgacat cgtgcgtggc tcagtgatca catcggtcat gttccggaca
     1801 cctccgagta gacgaaacgg gtgagatcgt ccaccgcctc ccagctacct gcttcggcga
     1861 actcgactgc gacgtcgatc tcctcggcga cctccttctc catcatggac caggcgtcgt
     1921 cagtgagcac ccggtccgca aggagccggt cacgaaatga ctcgatgggg tcgcgatgct
     1981 tccagctctc gacttcgtcc ttggaccggt acaactcggg atcgaacatg gaatgggccc
     2041 gatagcggta agtacgaagc tccagaaagt gtggccctcc acccgacctg accgactccg
     2101 ccgcggcacg ggctgccttc tccaccgcca ggacatccat gccatccacc tcccaggcag
     2161 gcatctgata gctcgccgcc ttcaaggtga tccgggtctg agactccgat ctgtccaggg
     2221 cggtgcccat ggcatagagg ttgttctcac agcagaagag aaccggcagg ttccacaacg
     2281 cggccaggtt catcgcctca tggaattcgc cttcggcgac ggcgccttcc ccgaagaaca
     2341 cggccgtgac ccgtgggctc aggctcatct tgtccgccag agccaggccg accgcgagcg
     2401 gtagaccgcc gccgacgatt gcgttccccc cgtagaagcg ggtcgacgag tcgaagagat
     2461 gcatcgagcc tcctctaccc cggctacagc cttcgacctt tccaaacatt tcagccatca
     2521 ccacccgagg atccattccc cgagccaggg catggccatg ctcgcgatag gtggcgacga
     2581 cggcatcttc ggggcccaga gcttgcatga cgccggtggc gacggcctcc tcgccgatgt
     2641 agagatgtag gaaccctctg atcttctcct gttgatagag ctgggcgcat cgctcctcga
     2701 agcgtcgaat ccgcagcatc tgtcggagca agacgagatg atgctcgccg atgctcattg
     2761 ctcctctcct tccagggtcg aagtgtcacc ggtgggcaaa cccagctccc aggccttgag
     2821 cagacggcgc atgatcttcc ccgagcgggt atggggcagg tcctggcaga gctcgacctc
     2881 ccgtggagcg acggaggcgc ccagcttgac ccggccccag ccgatcagct cctttcgcaa
     2941 ctcgtcagac cactcgtatc cgtcgttcaa ggcgacgaag gccttgacca cctcaccggc
     3001 caccgggtcc ggtttgccga tgaccccagc ctcggcgact gcttcgtgct cgatgagcgc
     3061 cgattccacc tcgaacgggc cgatgagatg gcccgcggac ttgatgacgt cgtcgccacg
     3121 gcccacgaac cagaagaaac cgtcctcgtc gcgctcggcg aggtctccgg agcagtacca
     3181 accgccgacg aagctcttgg catacctctc ctccatcccc agatagccac gaaacatcga
     3241 aggccagccg gggcgaaggg cgagctcacc ttcggtcaag gggatcgact cttcctgtgc
     3301 tgtgccatcc gggctgagcc ggacctctcc cttgtcgtcg cgtcggagga ttgcggcttc
     3361 gatgccaggg agaggccggc ccatggagcc gggtcggatc tgctcgccga tgtagttggc
     3421 gatcatgatc cccccggtct cggtctgcca ccagttgtca tggattggca gaccgaagac
     3481 tctttgaccc cagacgacgc cctcggggtt gagcggttcg cccacgctgg cgacgaatct
     3541 gagggaggac aagtcgaacc cgcgtgccac ctcctcgccg gccatcatca gcattcgtat
     3601 cgcggtgggg gcggtgtacc agacactcac cttctcctgt tcgatgatgc gataccagcg
     3661 ctccgcgtcg aactcgccct cgtcgacgat tgaggtcacc ccgtgcccca tcggagcgac
     3721 gattccatag gacatgcctg tcacccatcc cgggtcggcg gtgcaccagt agatgtcatc
     3781 ggggtgcagg tcgagtgcgt agagagcgga gacgtagtga tgcaggaccg cgccatggac
     3841 atggactgct cccttcgggg ttcccgtggt gccactggtg aaatggagca gagccatctc
     3901 ctcggggtcc atcggctccg ccgccgtgtc cgggtctcct tctttcagca ggtcttcgaa
     3961 ggcaaccgtg tccgtgccct cggacccgat gacgatcaca tgctcgagcc ccggcagctg
     4021 gctgcgaatc cccgcaacct tgcgtcggta gagcgtctcg gtcgtgacca ggaccttgcc
     4081 cgaacccagt tccatccgtg tctgtatggg ctcgggaccg aacgccgaga aaagcggcga
     4141 ggcgacgctt ccgttcttga gggcaccgag tgctgcgatg tagagctcgg ggatcctcgt
     4201 agagaggagg aagacgcgat cacccctgcc gacgcccaac ctgcggagac ccgacgcgaa
     4261 tcgggaggtg gcttcgccga gctctccgta cgtgtagtcc cgacgatcac cttttcgtcc
     4321 caaccagcga atcgcgggat gatccccatg gtcgggcaaa tggcgatcga tcgcttcgtg
     4381 cgcaatgttc agtttgcccc ctgggaggcc atcgaggtat ctctccgcct ccgaccagga
     4441 aaaagcagcc cgcgctctct cataatcctc gagattcgga ggcgtccgaa aaggtcccga
     4501 cttgtcgatg gccggatact gcgtctccat acggctcaac gtgccacaag gcgttcatcg
     4561 gcgcataggg ccaaaatgcc cctgttcgag actcaaaagt ccgattgggc cctagccagg
     4621 tgactatcgc ctctagtcag ctcgtcacgg ctggagcgat gctgggcaca tggacactcg
     4681 catcatcgac accaagtcga taggaagccg caagacagcg gttcgcattg ccgaactcga
     4741 cgtcgaagcc cttgctgagt ggttgccaga gacctttggg atcgtcgctc gccacctcga
     4801 cgagacaggc gacccaccca ttggcccacc ctttgctcgt tatcgccggc tcggaccccg
     4861 acgattccac gtcgaagccg ggtttccggt cggtcgtgcg atctatggaa cggacgagat
     4921 cgtcggttcc agtcttcccg gcggcaccgt tgcgatcacg acccacgtcg gtccgtacga
     4981 cgggatggag gccacctacg aggccctggc cacgtggctc gactcgagag gctcggtccc
     5041 catgggtgac ccgtgggagg tctacttcag cgagccgacc gatccgccca gcatgtggcg
     5101 caccgacata gtgcagcctt acctcaagcc ctgacccggg ccgggtccta caagggcggg
     5161 accaattcct ctgggcgaga acgtccgaat ggcggacgat cgcggcatga gacgcagagt
     5221 catattcctc tccgggttgg tggccgtgat gcttctgcta tctggctgcg gctcggacac
     5281 cggcgaaccc acgaccaata cagaggaggt gatcacgtcg acgtcagcgc cgacaacgac
     5341 caccaccacc gcg
//
LOCUS       DASUTA010000097         2848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_33190, whole genome shotgun sequence.
ACCESSION   DASUTA010000097 DASUTA010000000
VERSION     DASUTA010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33190"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1646
                     /locus_tag="VFZ80_01925"
     CDS             <1..1646
                     /locus_tag="VFZ80_01925"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX6286217.1"
                     /translation="RVFGEMLWRLADMVRAGETRRSFRAKAFQRAVWALDHLPPDLRF
                     DKEKALAISGIGPGVLSLIEEYLNRGRLTELETLERRLPSEAPHLRRLPRMTPKILTE
                     LKAAIGVETRADLRVAIESDSLGSISGVGPATMALWAEILALQPRDSTIVPSHSGWVI
                     ASMLARHISSHTGCWIDIAGDVRRLEEWVGGVDLVATGGSVDALADFLLDSAVLGDAE
                     REDDRVTGRAHTGLPVDILWAPPDVAGTALAQMTGPQAHLDHLSLDPYPTENELYEAS
                     GFPLIPAPARVLPIDVGSRVVLRQDLRGDLHLHTEDSPDGHLSIDALLNAAVAESYDY
                     VLITDHTQGLRFGGLDEQAMLQQAAAIERMRPRFPQIVVLHGAELNIAVDGSLDMDDD
                     TLSILDFAVAGLHSHFGLDEHAQTRRLETAMAHPAVRVLAHPFGRRIGIRPAIDVDME
                     TVIEASLRHGVALETNGHRDRLDLGADWLEIAAERGALFTANSDAHRREEIGNVANAV
                     GTLQRAGIDAERVVNTWDVDRLLAWIGDDHTNRRVTKTV"
     gene            complement(1680..1769)
                     /locus_tag="VFZ80_01930"
     tRNA            complement(1680..1769)
                     /locus_tag="VFZ80_01930"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1733..1735),aa:Ser,seq:gct)
     gene            complement(1886..1974)
                     /locus_tag="VFZ80_01935"
     tRNA            complement(1886..1974)
                     /locus_tag="VFZ80_01935"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Ser,seq:tga)
     gene            2128..2463
                     /locus_tag="VFZ80_01940"
     CDS             2128..2463
                     /locus_tag="VFZ80_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286218.1"
                     /translation="MSNLTDPQFLRPIERRVLDMHNGDVPLDEIATRFRKTPEFVERL
                     IGWTEIPRNGSPTERQLTPIQRRVLDLRADGESHSEIAAKFKKSERFIRQVEGLAHFK
                     EAQRLLSRA"
     gene            2556..>2848
                     /locus_tag="VFZ80_01945"
     CDS             2556..>2848
                     /locus_tag="VFZ80_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286219.1"
                     /translation="MASLAALLTYLLDPDRGRARRARFADQLSAKGRDISDTVAAKAK
                     YQKGVFEGVAHEVASVFDEEKAYDDETLMQKVRSEAVGPSGVDAGDVVIDITDG"
BASE COUNT          540 a          912 c          875 g          521 t
ORIGIN      
        1 gcagggtctt cggcgagatg ctctggcgtc tggcggacat ggtgcgggcg ggcgagacac
       61 gaaggtcatt tcgtgccaag gcctttcaac gagctgtctg ggcgctcgac catctaccac
      121 cagacctgag attcgacaaa gagaaggcac tcgcaatttc agggatcggg cccggagtcc
      181 tctcgctcat cgaggagtac ctgaatcggg gacggctcac cgagctggag accctggagc
      241 ggcggcttcc cagcgaggcc cctcaccttc gtcgactccc tcgcatgacc ccgaagatcc
      301 tcaccgagct caaggcagcc atcggcgtcg agacgagagc ggacctccgc gtggcaatcg
      361 agtccgactc gctcggctcg atctccggcg tggggccggc caccatggca ctgtgggctg
      421 aaatcctcgc tctacagccg cgggactcca cgatcgttcc ctcacactcc ggttgggtca
      481 tcgcgtcgat gctggcgaga catatcagct cgcacacggg ctgctggatc gacatcgccg
      541 gggacgtgcg tcgcctcgag gagtgggtgg gtggcgtcga cctcgtcgca accggcggtt
      601 cggtcgatgc gttggcggat ttcttgctcg attccgccgt cctcggcgac gccgagcgtg
      661 aggatgacag agtcactggc cgtgcccaca ccgggctgcc ggtcgacatc ctctgggccc
      721 ctcctgacgt ggcggggacc gccctggccc agatgacagg tccacaggcg catctcgacc
      781 atctcagcct ggacccatac ccgaccgaga acgagctgta cgaggcgtcc ggattcccct
      841 tgatcccggc tccggcccgg gtcctgccga tcgacgtggg ctcgagggtg gttcttcgcc
      901 aggatctcag aggcgatctc caccttcaca ccgaggactc tccggacggc catctctcga
      961 ttgatgcgct gctgaacgca gcagtggctg agagctatga ctatgtgctc atcaccgacc
     1021 acacccaggg cctccggttc ggcggcctcg atgaacaggc gatgcttcag caggccgccg
     1081 ccatcgagcg gatgaggccc cgctttcccc aaatcgtcgt cctccacggt gcggagctga
     1141 atatcgcggt tgacggctct ctggacatgg acgacgacac cttgtcgatt ctcgatttcg
     1201 ccgtcgccgg cctccactcc cactttggcc tcgacgagca tgcgcagacg agacgtctcg
     1261 aaacggcgat ggcacatccg gccgtcagag tgctcgcaca tccgttcggg cgtcggatag
     1321 gaattcgtcc cgccatcgac gtcgacatgg agacggtcat cgaggcttcg ctccgccacg
     1381 gcgtcgccct cgagaccaat ggtcatcgtg accgtctaga cctcggagcg gattggctcg
     1441 aaatcgctgc cgagagaggt gccctcttca ccgccaattc cgacgcccac cgccgggaag
     1501 agataggcaa cgtcgccaac gcggtgggaa cgctgcagcg cgccgggatc gacgcggagc
     1561 gagtggtcaa cacatgggat gtggacagac ttctcgcctg gatcggcgat gaccacacga
     1621 accggcgagt aacgaagacg gtatagccgt gttcattgca cgtgagtcgt gatgaagagc
     1681 ggagagggag ggatttgaac cctcgaagag gcttataacc ccttactcgc ttagcaggcg
     1741 agcgccttcg accactcggc cacctctcca gatcgtcgag cttcacccga cgagtacaag
     1801 gtacttgcta ctcggtactt ggcccagccg actcccgatg agccagccgc cgccactctc
     1861 ggcgccagac gacatccggt acacgcggag ggggtgggat tcgaacccac gagaccccgt
     1921 gaggggccca acggttttca agaccgtctc cttcgaccgc tcggacaccc ctccggtcgc
     1981 tgaaccctga tcagggctgt gagtcgcctc gggaccgtgg ctcgaggtca tcagggtaat
     2041 cggatcaagc tgatctcgat agccgcgcac gccctggttc cggcggtttt gacagggatc
     2101 ggcgccgcag gcactaggtt tgtccatatg agcaacctca ccgaccccca gtttctgcgg
     2161 ccgatcgaga gacgggtgct cgacatgcac aacggcgacg ttcctctcga tgagatcgcc
     2221 accaggttca ggaagacccc tgagttcgtg gagcgtctaa tcggctggac cgagatacca
     2281 cgcaacggca gtcccactga gagacagctg acaccgatcc agcgtcgggt cctcgacctt
     2341 agagccgatg gtgagtctca ctccgagatc gccgccaagt tcaagaagag tgagcgcttc
     2401 atccgccaag tcgagggtct tgcccatttc aaagaggccc agcgtctgct ttctcgcgcc
     2461 tgagtcgacg gtttcgcaga aacctgcaac gggtatctga atcaccagcc catctctgga
     2521 ggtttctgat gcgaagattt ctatggatcg cgggtttggc gtcgctcgcc gccctgctca
     2581 cctatctgct ggaccctgat cggggacgcg cccgtcgagc tcgattcgcc gaccagcttt
     2641 cggcgaaagg cagagacatc agcgacacgg ttgccgccaa ggccaaatac cagaaaggtg
     2701 tcttcgaagg tgtggctcat gaggtcgcca gcgtgttcga tgaggagaag gcgtacgacg
     2761 acgagaccct catgcagaag gtgcgcagtg aggcagtcgg tccgtccggc gtagacgccg
     2821 gtgacgtcgt gatcgatatc accgacgg
//
LOCUS       DASUTA010000098         2916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1846738, whole genome shotgun sequence.
ACCESSION   DASUTA010000098 DASUTA010000000
VERSION     DASUTA010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846738"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFZ80_01950"
     CDS             complement(<1..525)
                     /locus_tag="VFZ80_01950"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015228668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0044210 - 'de novo' CTP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine monophosphate kinase"
                     /protein_id="HEX6286220.1"
                     /translation="MTQPSNRRVLLKLSGEAFADPEVGYGIDPIMVHRVASEIAEAQK
                     NGVEVAVVVGGGNIFRGVSQAARGMDPANADYMGMLATVINALALRDALENAGVPTRV
                     QSAITMQELAEPYIRLRAIRHLEKGRVVIFAAGTGNPFFTTDTTAALRAIEIGAEVLL
                     KASKVDGLDDSDPKT"
     gene            complement(534..1145)
                     /gene="tsf"
                     /locus_tag="VFZ80_01955"
     CDS             complement(534..1145)
                     /gene="tsf"
                     /locus_tag="VFZ80_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX6286221.1"
                     /translation="MSEITAKDIQKLRQQAGVGMMDAKKALTDADGDFDKAFELLRER
                     GQAKMAKRADREASDGTIGSYIHIQNDRPVLGVLVELSCETDFVAKSEEFREVADDIA
                     MHVSWASPGWVTRDEVDEDVIAKERDLIGRQAKAEGKPDDVVERIVDGKIESWYGENV
                     LYDQKFVNPERYDGTVGDMVGALGAKMGENISVRRVARVAVGG"
     gene            complement(1165..1914)
                     /gene="rpsB"
                     /locus_tag="VFZ80_01960"
     CDS             complement(1165..1914)
                     /gene="rpsB"
                     /locus_tag="VFZ80_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX6286222.1"
                     /translation="MAEVTMKQLLEAGVHFGHQTRRWDPKMRPYIYGERNGIHIVDLR
                     QTVTQVADAIEFVKQIAIDGGSVLFIGTKKQAQSAVEEAARRSGMPFVNYRWLGGMLT
                     NFATIQKRVYYMRELRRLEDTGELNARPKKERLKLRRELEKLEQNLGGVSEMTRLPDA
                     VFIVDVNTEETAVREADRLGIPIIALVDTNSDPDPVDYVIPGNDDAIRAADLIASALA
                     DAIVEGKEIASKKKPAKAADDEVDAKAGAEA"
     gene            2130..>2916
                     /locus_tag="VFZ80_01965"
     CDS             2130..>2916
                     /locus_tag="VFZ80_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HEX6286223.1"
                     /translation="MPIADNRTIRQTALAAGADTVGFTSADPFPDALTTMRRRKGKGM
                     AGPLHFTYEDPELSTDIRQTFPWARSLVVLGASYIEQAPQPAASGPVVALFATRDHYQ
                     RVRDAAAAVITLIEDAGGKADMVVDDDRFVDRAAAVRAGVGWIGRSTMVLTPGHGPWQ
                     LFGSIVTDLDLDEDAPMRRTCGTCVACIPACPTGAITEDGVDARLCISTWLQSSGSIP
                     HWVRPLIERRIYGCDDCLTSCPPGSPAMAAARVEVETHSFSELL"
BASE COUNT          466 a          954 c          891 g          605 t
ORIGIN      
        1 ggtcttcggg tcggagtcgt cgagcccgtc caccttggac gccttcaaga ggacctcggc
       61 tcctatctcg atggcgcgaa gggctgctgt ggtgtcggtg gtgaagaacg gattccctgt
      121 gcctgccgcg aatatgacga ctcgaccctt ctccaaatgt cttatggcgc gcagccggat
      181 gtacggctcg gccagctcct gcatcgttat ggcgctctgc actcgggtcg ggacaccggc
      241 gttctccagg gcatcgcgaa gagccagggc attgatcacc gtggccagca tccccatgta
      301 gtcggcgttg gcgggatcca tcccccgggc agcctgggag acgccgcgga agatgttgcc
      361 tccgcccacg acgacggcga cctcgacacc gttcttctgg gcttcggcga tctcggatgc
      421 gacccgatgc accatgatcg ggtcgattcc atatccaact tcgggatcgg cgaaagcctc
      481 tcccgagagc ttcaggagta cgcgtctgtt ggatggctgc gtcaagctcg ctcctatccg
      541 cccacggcga ctctcgccac gcgtcgcacc gagatgttct cacccatctt ggcgccgagt
      601 gcgccgacca tatcgccaac ggtgccgtcg tagcgctccg ggttgacgaa tttctggtcg
      661 tagaggacgt tctcgccgta ccacgactcg atcttgccgt ccacgatgcg ctcgaccacg
      721 tcgtctggtt tgccctccgc cttcgcctgg cggccgatca agtctcgctc cttggcgatg
      781 acatcctcgt cgacctcgtc gcgggtgacc catcccgggc tggcccaact cacgtgcatt
      841 gcgatgtcat cggcgacctc ccggaattcc tcgctcttgg cgacgaaatc ggtctcacac
      901 gacaactcca cgagcacgcc caagacgggc cggtcgttct ggatgtgtat gtagctgccg
      961 atggtgccat ccgaggcctc gcggtcggcg cgcttggcca tcttggcctg gccccgctcc
     1021 cgcaacagct cgaacgcctt gtcgaagtcg ccgtcggcgt ccgtcagcgc cttcttggcg
     1081 tccatcatgc cgaccccggc ctgttggcga agcttctgga tgtccttggc ggtgatctca
     1141 ctcatcgtcg ttcggaatcc tcgctcaagc ctcggctccg gccttcgcat cgacctcgtc
     1201 atcggcagcc tttgccggct tcttcttcga tgcgatctcc ttaccctcga cgatggcatc
     1261 cgccagggcg ctggcgatca ggtcggcggc gcgtattgcg tcgtcgttgc cgggaatcac
     1321 gtagtcgacg gggtcggggt cggagttggt gtcgacgaga gcgatgatcg ggatgccgag
     1381 acgatcggct tctcggaccg cggtctcctc ggtattgacg tcgacgatga agacggcgtc
     1441 gggaagacga gtcatctcgg agactcctcc cagattctgc tcgagcttct ccaactcgcg
     1501 tcggagcttg agacgttcct tcttgggtcg ggcgttcaac tcgcccgtgt cctcgagacg
     1561 acgcagttcc ctcatgtagt agacccgctt ctggatggtc gcgaagttgg tgagcatccc
     1621 gcccagccat cgatagttga cgaacggcat gcccgagcgc ctggcagctt cttcaaccgc
     1681 cgactgggcc tgcttcttgg tcccgatgaa gaggaccgag ccgccgtcga ttgcgatctg
     1741 cttgacgaac tcgatggcgt ctgcgacttg tgtgaccgtc tgacgcaggt cgacgatgtg
     1801 tattccgttg cgctcgccgt atatgtacgg ccgcatcttg gggtcccagc gcctggtctg
     1861 atggccgaag tggacaccgg cttccaagag ctgcttcatt gtgacttcag ccacaactgc
     1921 tcctttctgg ttgttcctcc gcccggtggg ccgtgtcgac ctcgtcgtca gccggccccc
     1981 gcatccagag atgcagctcc tcttcaaccg gctcttgggg agcacggcac cagagaggta
     2041 gttcacgggc gtgcgtgatg gtggagattg tatgggcggt cgacgaaatt cagtaatcgg
     2101 cctcttgccg gggctggcac actcaggtgg tgcccatcgc cgacaatcgc accatccggc
     2161 aaacggccct ggccgccgga gcagacacgg ttggcttcac ctcggctgat ccattccccg
     2221 atgccctcac caccatgcgc cgtcggaagg gaaaggggat ggcggggccc ctccacttca
     2281 cgtatgagga tccagagctg tcgaccgaca tccggcagac ctttccctgg gcacgcagcc
     2341 tcgtcgtatt gggggccagc tacatagagc aagcaccgca accggccgcg tcggggccgg
     2401 ttgtcgctct cttcgccacc cgcgatcact atcaaagggt ccgagacgcc gctgcagcgg
     2461 tcatcaccct catcgaggac gccggaggca aagccgacat ggtcgtggac gacgacagat
     2521 tcgtcgatcg ggccgcagcg gtacgcgccg gcgtgggatg gatcgggcgt tccacgatgg
     2581 tgctcacacc tggtcacgga ccgtggcagc tgttcggctc gatcgtcaca gatctcgatc
     2641 tcgatgagga cgcccccatg cgacgcacct gcggaacctg cgtggcgtgc attccggcct
     2701 gtccgaccgg cgccatcacc gaggatggcg tcgacgccag gctctgtatc tcgacctggc
     2761 tgcaatcgtc ggggtcgatc cctcactggg tcagaccact catcgagcgg cgaatctatg
     2821 gctgcgacga ttgtctgaca tcatgcccac cgggttcgcc cgccatggcg gcagcaaggg
     2881 tggaggtgga gacgcattcg ttctccgagt tgctcg
//
LOCUS       DASUTA010000099         5599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_421110, whole genome shotgun sequence.
ACCESSION   DASUTA010000099 DASUTA010000000
VERSION     DASUTA010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421110"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..3388)
                     /locus_tag="VFZ80_01970"
     CDS             complement(50..3388)
                     /locus_tag="VFZ80_01970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEX6286224.1"
                     /translation="MNRSSNSPRYAELHCHTNFSFLDGASHPEDLVQRAMDLGYEALA
                     VTDHDGFYGTSRLWQAAKATGLPVIYGVEIGIEVDPNGPVDPIDTAEDWDRMRYARIK
                     GGQGRLRRGRSVRAHGAKPTDLPESDHLVLLSGSPDGYRSLSHLVTKAQMRGEKDHPI
                     YTWDDLAVVATNRDVHALTGCHNGAVPKAASTHDLGATIRETSRLREIFGTRLHLEMW
                     HHRMPEDDGRNDLMWEVGQKLGISTVATNQVHYHDRCDAYLSEVLAAIGGRRTLAEAD
                     GFRPATDERYLKHPREMEWRFRRYPGVVARSAELGRRLAFDLGLVAPRLPDFPMPGHF
                     RDEMDYLRHLTWEGARQVYPGSDDGIDPVARRRLDHELDIIERLGFPGYFLIVKDLVD
                     FSREQGIYCQIRGSGADSAVCRCIGLTRVDPIRLHLPFERFLSEERGKPPDIDIDFEA
                     DRREEVIQYCYRRYGRERAAMVANVITYRARSVLRDVAKTFGFTQAQVDGLSKFVDSH
                     DPAQLRMMDTSLPDGMTAEMIYDICWRLDGYPRHLGIHSGGMVIADRPLWQVVPLEWG
                     RLEGRTVIQWDKDDSAAVGIVKFDLLGLGMLNALHLATDLIEDTHGIEVDLALIPQEP
                     AIYESMTRADTVGLFQIESRAQMATLPKMKPKTFYDLAVEVALIRPGPIQGQSVHPYL
                     RRRNGEEPIRYPHPSTEEILEKTLGVPIFQEQLMELARICAGFNGSQADRLRQAMTHK
                     RSAEAMGRLRDEVYAGMAGNGITGPAADEIWEKLQGFASFGFPESHSVSFAYIVYMSA
                     WLRFHYPAEYLAGLLNAQPMGFYSPNSLVQDSQRHGVVVLGPDVNVSWHDCSIESIDA
                     DPEDIVTYLGQSWRRGRGHVDDPIRPAVGVRMGLRYVRNLGEREVTRIEAARILGGDF
                     SSPEDFAFRTGLDVEALEGLAASGALESIGLGRREGMWAAGALAEIDPERLALSPGVE
                     APPLPGMTEEEAHRADLWATSVSSRHPMSFVRHRLDGCLTAAETLEVKRNKSRVKVAG
                     VITHRQRPGTAKGVYFLNLEDETGLLNIVVLPDVWAKHRNVVRKSPAMVIYGRLEFYD
                     GVTNIVARDFEPIGVQTVQSRDFR"
     gene            complement(3433..4317)
                     /locus_tag="VFZ80_01975"
     CDS             complement(3433..4317)
                     /locus_tag="VFZ80_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX6286225.1"
                     /translation="MRVGALAVVAVLMAACSDGGLEPATTGSVLPSATTTTAPLTRVP
                     SSTTTTVAPTTTTTINPFARPDWLGTRPLPLRPDGHGEVQETPEELVDRQLETIDLLE
                     PPLDGEWAATITPVPDDVLGRSSWVEGCPVAVDELRYVTMSHHGFDGEVHTGEMIVHV
                     DEAKGVVEVFRRIFDAGYPIEQMRVIRQDEVDDPPTGDWNETTSFVCRPAVGSNNWSQ
                     HAFGLAIDVNPFQNPYVRDDLVIPELASAYTDREEVRAGMIFRDDVVVKAFAGIGWSW
                     GGNWNTLKDWMHFSRSGR"
     gene            4356..5504
                     /gene="nagA"
                     /locus_tag="VFZ80_01980"
     CDS             4356..5504
                     /gene="nagA"
                     /locus_tag="VFZ80_01980"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEX6286226.1"
                     /translation="MASGARPWLNEDVTTIRNGKLAIDGELIERDLHITDGFIAEESA
                     DDEVIDATGMIVSPGLIDIQINGGFGHDFTQDPTTIWEVGRLLPELGVTAFVPTIVTS
                     PDAVTDLALDVVANRRPEDYVGAEVLGLHFEGPWISPDRHGAHNRDHIVDPNIDVAEK
                     WAQSGLVRIVTLAPERPHAAEVAEMLAAAGVVVSIGHSDANYETARDSLSGPASLGTH
                     LFNQMSPFTHRRPGVVGALLLSGRPAIVIVDGLHIAAGALELAWRMLGPERTVLVTDA
                     MAALGLGPGTYPLGDGPITVGEDGPRTSDGRLAGSVGTLPEAVSNLAETTGASIAEAL
                     LGATANPARVLGLEDRGHLRVGHRGEIVVFDNSLQPMKTLLAGKVVDL"
BASE COUNT         1027 a         1784 c         1766 g         1022 t
ORIGIN      
        1 tgccatagca agaccgagcg gaaaccgctg caccacgcta tagcaacgcc tatctgaagt
       61 cccgggactg aaccgtctgc actccgatcg gctcgaagtc gcgggcgacg atgttggtga
      121 caccgtcgta gaactcgaga cgaccgtaga tcaccatggc ggggctcttt ctgaccacat
      181 tgcgatgctt ggcccacaca tcgggcagca caacgatgtt cagcaatccg gtctcgtcct
      241 cgaggttcaa gaaatagacg cccttggccg tgccgggtcg ctggcggtgt gtgatcacac
      301 cggccacctt gaccctcgac ttgttccgct tcacttcgag ggtctcggcg gcggtgaggc
      361 acccatcgag acgatgccgg acaaagctca tcgggtgcct cgaggagacc gaggtggccc
      421 acagatcggc acggtgggcc tcctcttcgg tcatccccgg cagcggaggc gcctccaccc
      481 ctggagacag ggccaaccgc tcggggtcga tctcggcgag ggctccggcg gcccacatgc
      541 cctcacgacg ccccagcccg atcgactcca aggctcccga agccgcgagt ccctccaacg
      601 cctccacgtc gagacccgtg cggaaagcga agtcttctgg tgaagagaaa tcacctccca
      661 gtatccgagc tgcctcgatt cgagtcacct ctctctcccc caggtttctc acatagcgca
      721 gacccatcct cacccccacg gcggggcgga tcgggtcgtc gacatggccc cttccccgtc
      781 tccagctctg accgagatag gtgacgatgt cctcggggtc ggcatcgatc gattcgatcg
      841 agcagtcgtg ccaggagacg ttcacatccg gcccgagcac gacgacgcca tggcgctggc
      901 tgtcctgaac cagtgagttc gggctgtaga agcccatcgg ctgggcgttg agcaacccgg
      961 ccaggtactc cgccgggtaa tggaatcgaa gccatgccga catgtagacg atgtaggcga
     1021 agctcaccga atggctctcg gggaagccaa agctggcgaa gccctggagt ttctcccaga
     1081 tctcgtcggc tgccggcccg gtgatgccat tgcccgccat gcctgcatag acctcgtcgc
     1141 ggagacggcc catggcctct gccgagcgtt tgtgggtcat ggcttgacgg agccgatcgg
     1201 cctgtgatcc gttgaacccg gcacatatcc gcgccaactc catgagctgc tcttggaaga
     1261 tggggacacc gagggtcttc tccaatatct cctcggtgga gggatgcggg tatctgatcg
     1321 gctcttcccc gttacggcga cgcagataag ggtggaccga ctgaccctgg atcgggccgg
     1381 gcctgatcag cgccacctcg accgccaagt cgtagaaggt cttcggcttc atcttgggca
     1441 gggtcgccat ttgcgcccgg gactcgatct ggaacagccc gaccgtgtcg gccctggtca
     1501 tcgactcata gatggcaggc tcttggggaa tgagggcgag atcgacctcg atcccgtggg
     1561 tgtcctcgat gagatcggtc gccaggtgga gggcgttcaa catgcccaga ccgagcaagt
     1621 cgaacttgac gatccccacc gcggccgagt cgtccttgtc ccattggatg accgttctcc
     1681 cttcgagacg accccattcc agtggaacga cctgccacaa gggacggtcg gcgatgacca
     1741 tgccacccga gtggatgccg agatgtcgtg gatagccgtc tagccgccag cagatgtcgt
     1801 agatcatctc ggcggtcatc ccatcgggca gcgaggtgtc catcatcctg agctgtgccg
     1861 ggtcgtggga gtcgacgaat ttggacagcc cgtcgacctg cgcctgagtg aacccaaagg
     1921 tcttggcgac atcgcgcaga accgagcgtg cccggtaggt gatgacgttg gccaccatcg
     1981 ccgcccgctc cctcccatag cgccggtagc agtactggat cacctcttcg cgccggtccg
     2041 cctcgaagtc gatgtcgatg tcagggggct ttcccctctc ctcggagaga aagcgctcga
     2101 aggggagatg gagccgaatg gggtcgacgc gggtgagccc gatgcaacga caaaccgccg
     2161 agtcggcgcc cgagcctctg atctggcagt agatcccctg ttcccgcgag aagtcgacga
     2221 gatccttgac gatgaggaag tatccgggga accccagtct ctcgatgatg tccagctcat
     2281 ggtcgagacg acgacgggct acagggtcga tgccgtcgtc cgacccggga tatacctggc
     2341 gcgctccctc ccaggtgaga tggcgtagat agtccatctc gtcgcggaaa tggcccggca
     2401 tcgggaagtc aggcagccgg ggggcgacga gacccaggtc gaaggccagc cgccggccca
     2461 gctcggcgct gcgtgccacc acacctggat agcggcggaa cctccactcc atctctcggg
     2521 ggtgtttgag gtatcgctcg tccgtggcgg ggcgaaatcc atctgcctcc gccagagtcc
     2581 tccttcctcc gatggcggcg aggacttcgg acaggtaggc atcgcagcga tcgtgatagt
     2641 gaacctggtt ggtggcaacg gtggagatcc cgagcttctg gcccacttcc cacatgagat
     2701 cgttccgccc gtcgtcctcg ggcatgcggt gatgccacat ctcgagatgg agacgtgtcc
     2761 cgaagatctc gcggagacgg gatgtctccc ggatcgtcgc ccccaggtca tgtgtggatg
     2821 cggctttggg caccgcaccg ttgtgacatc cggtgagggc atggacatcc cggttggtgg
     2881 cgacaacggc gagatcgtcc caggtgtaga tggggtggtc tttctcgcca cgcatctgcg
     2941 ccttggtgac cagatgcgac aggctccgat atccgtccgg cgacccggag agaaggacga
     3001 gatgatccga ttctgggagg tcggtgggtt ttgcgccgtg ggctctcacc gagcgcccac
     3061 ggcggagccg tccctgaccg cccttgatcc gggcgtatcg catcctgtcc cagtcctcgg
     3121 cggtgtcgat gggatcgaca ggaccattcg ggtccacttc gataccgatt tccaccccgt
     3181 atatgaccgg caaccctgtg gcctttgccg cctgccagag tcgggaggtc ccgtagaagc
     3241 catcgtgatc ggtgaccgcc agcgcctcgt agccgaggtc catcgctcgt tgcaccagat
     3301 cctcgggatg tgaggcaccg tcgaggaagg agaagttggt gtgacagtgc agttcggcgt
     3361 agcgagggct gttcgaactg cggttcatgc ggccactata tcgaacacat gttcgagtaa
     3421 gccggtcgac agctaacggc cgctgcggga gaagtgcatc caatccttca gcgtgttcca
     3481 gttgccaccc caggaccagc cgatgcctgc aaaagccttc accaccacat catctcgaaa
     3541 gatcatccct gcccggacct cttccctatc ggtataggcc gacgccagct cggggatcac
     3601 cagatcatcc cggacatagg ggttttggaa gggattgacg tcgatggcca gaccgaatgc
     3661 atgctgggac cagttgttcg atccgacggc aggacggcag acgaatgacg tggtctcgtt
     3721 ccaatctccg gtggggggat cgtccacttc gtcctgtcgg atcactctca tctgctcgat
     3781 gggatagccg gcatcgaaga tcctcctgaa gacctccacc acccccttcg cctcgtcgac
     3841 gtggacgatc atctcgccgg tgtggacctc gccgtcgaat ccgtgatgac tcatcgtcac
     3901 atatcggagc tcgtctacgg cgaccgggca gccctcaacc cacgaggacc tgcccaggac
     3961 gtcgtcgggc accggagtga tcgtggccgc ccactcaccg tcaagcgggg gctcgaggag
     4021 gtcgatcgtc tccaactgac gatccaccag ctcctcaggc gtctcctgaa cctcaccgtg
     4081 gccatccggt cgcaaaggca gtggacgggt tccaagccag tcggggcgcg caaaggggtt
     4141 gatcgtcgtg gtggttgtcg gggcgaccgt cgtagtggtc gaagacggca ctctggtgag
     4201 aggtgctgtc gtggtcgtgg cacttggcag gaccgagcct gtggtagccg gctcgagacc
     4261 gccgtcggaa caggcggcca tcaggaccgc tacgaccgcc agcgctccga ctcgcatgcc
     4321 ccgacgatag aaaacatcgg tgcccacggc gtcggttggc gtcgggcgcc cggccgtggc
     4381 tcaatgagga tgtgaccacc atccgcaacg ggaagctggc catagacggc gagctgatcg
     4441 agcgagacct tcacatcacg gacggcttca ttgccgagga atcggcagat gacgaggtca
     4501 tcgacgccac cgggatgatc gtctcccccg ggctgatcga catccagatc aacggcggat
     4561 tcggccacga cttcacccag gacccgacca ccatctggga ggtcggcagg ctgctgcccg
     4621 agctcggggt caccgccttc gtgcccacga tcgtcacatc acccgatgcc gtcaccgacc
     4681 tggctctcga cgtggtcgcc aatcgcaggc ctgaggacta cgtcggggcc gaggtcctcg
     4741 gtcttcactt cgaggggcct tggatatccc ccgataggca cggtgcccac aatcgcgacc
     4801 acatagtcga cccgaacatc gacgtggcgg agaaatgggc ccaatccggg ctggttcgga
     4861 tcgtgacgct ggctccggag cgtcctcacg ccgccgaagt ggccgagatg ctggctgcag
     4921 caggtgtggt cgtttccatc gggcacagcg atgccaacta cgagaccgcc cgggactcgt
     4981 tgtcaggccc cgcttcgctg ggcacacacc tcttcaacca gatgagcccc ttcacacacc
     5041 gccggccagg tgtggtggga gccctgttgc tatcgggcag accggccatc gtgatcgtcg
     5101 acggactgca catcgcagcc ggcgccctcg agctggcgtg gcgcatgctc ggcccggagc
     5161 ggacggtgct cgtcaccgac gccatggcgg cgctcggtct cggacccgga acgtatcccc
     5221 tgggcgacgg ccccatcacg gtgggcgaag atggcccgcg cacctccgac gggcgtttgg
     5281 ctggaagtgt tggcacgctt ccggaggcgg tgtcgaatct tgccgagacc acgggggcga
     5341 gcatcgccga ggcactgctc ggcgccacgg caaatccggc tcgggtgctc gggctcgaag
     5401 accgaggaca tctacgggtt ggacaccgag gcgagatcgt cgtcttcgac aactcactgc
     5461 agccaatgaa gaccctcttg gccgggaagg tggtcgactt gtaacgttga tgcccacacc
     5521 ggtgtcgaat acgttggtag cttggctcgc gggatcgaag gaggaccact gatggatgga
     5581 atagtcggaa cgatcgtct
//
LOCUS       DASUTA010000100         2636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_811008, whole genome shotgun sequence.
ACCESSION   DASUTA010000100 DASUTA010000000
VERSION     DASUTA010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811008"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..1013
                     /locus_tag="VFZ80_01985"
     CDS             279..1013
                     /locus_tag="VFZ80_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEX6286227.1"
                     /translation="MTFREGRLVRVMVSMADTLVSEFDAVDLFYELVMSCTDLLEVAQ
                     AGLLLTDGNGELRVVAASSEATGVLELLELENHGGPGAEAFRTGRPVRSGELAGSQAL
                     HRWPDFGEAALSEGFRSVTAVPMRLRDQVAGALDLFLTEAEDLSDEDLTVAQALADLA
                     TIAILQDRSTIDDRTLISQLRTALETRVLIEQAKGIVAHEAGLNMDEAFGHIRNHARN
                     NNERLRDISAAIVSGDLTPARLTSGD"
     gene            1229..1513
                     /locus_tag="VFZ80_01990"
     CDS             1229..1513
                     /locus_tag="VFZ80_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286228.1"
                     /translation="MIEVQGELDLFTGPMLHQHLESYNDPSGNNGHPRRVIYLLPELK
                     FMDARGLHYLLTAFDGHGSETITIREPSSMVCRLLELVGLDSMIEERANR"
     gene            1510..2193
                     /locus_tag="VFZ80_01995"
     CDS             1510..2193
                     /locus_tag="VFZ80_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEX6286229.1"
                     /translation="MISPSDLAALRESFTSWDQPGLPSSLATDCVRLLDVSDITISFV
                     GASGQHILCTSSDDSRTLDEWEFTLQEGPCFDAGVNSTSKTAETAPSNTNPWPQLSAK
                     AHAIGYQSIAGVPLRAGGTTFATLNLQDRHGKITPETLADAEQIAKELASLVVGSLSH
                     QPPSPDQPVEHDTFHQATGMVMAQMGIDSGSAADVLRAHAWAHDRLLMDIASEVVART
                     LTFSPASPR"
     gene            complement(2436..2508)
                     /locus_tag="VFZ80_02000"
     tRNA            complement(2436..2508)
                     /locus_tag="VFZ80_02000"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2473..2475),aa:Asn,seq:gtt)
BASE COUNT          535 a          862 c          740 g          499 t
ORIGIN      
        1 cggccattga tccttaccga ggtagcggcg ggtcagtgac ctcccattcg tcatgcctgt
       61 tcggccagta accgatccga cgagccggag aatggagggg tcgtctggga acatccccta
      121 cgaggtcggc tcctcgtttg atctctccgc atcagccttt cgagtgggct cctgtgaggc
      181 gagtccacgg atatgagctc cctgaggatt ccacgagggc ccttcagacc acggacccag
      241 tttcatcact cgatggcgct tacgtctagc ctctcgccat gacgtttcga gagggtcggt
      301 tggtccgggt gatggtgagt atggcggaca cgctggtctc ggaattcgac gcggtcgact
      361 tgttctatga gctggtgatg agctgtacgg atctattgga agtggctcag gcggggttgt
      421 tgttgacgga cggaaacggt gaattgcggg tggtggcggc gagcagcgaa gccaccggtg
      481 tgctcgagct cttggagctg gagaaccatg ggggtcccgg tgcggaggcg tttcgtaccg
      541 gccggccggt cagatccggg gaactggctg gatctcaggc ccttcacaga tggccagatt
      601 tcggagaggc ggcactttcg gagggattcc gctcagtcac ggctgttccg atgcgcctcc
      661 gcgaccaggt cgcaggggcg ctcgacttgt tcctgacaga ggcagaggat ctttctgacg
      721 aggacttgac cgtcgcccaa gccttggcgg atctagccac catcgccatt ctccaggacc
      781 ggtcgaccat cgacgaccgg acgctgatct cccagctccg gacggctctc gagacccggg
      841 tgttgataga gcaggccaaa ggaatcgtcg cacatgaggc cggcctgaac atggacgagg
      901 cgttcgggca catccggaat catgcccgca acaacaacga gcggctgcga gacatctctg
      961 ccgccatcgt ttcgggcgac ttgacaccag cccgcctcac ctctggcgat tagggctggt
     1021 tttcccgctc ttcccataga gcagaagccc ctgtagagtg gtagacaagc gcaaccaatg
     1081 agtctgctct agatcctggc ggaccctcaa ccatctaagg aggggccagg acatgaccgt
     1141 cagcactccc gtatcgagag cgggtttatc gggttacgag cctcctcctc tggacgtttc
     1201 actgagaagc gacgatcctt ccacttttgt gatcgaggtg cagggcgagc tcgacctctt
     1261 caccggccca atgctgcatc aacacctcga gtcctacaac gatccgagcg ggaacaatgg
     1321 tcatccgcgg agagtcatct acctactccc cgaactgaag ttcatggacg ccagaggcct
     1381 gcactacctt ctcaccgcct tcgatggaca cggttccgag acgatcacca tacgagaacc
     1441 gtcgtcgatg gtctgccgac tactcgagct cgtcgggttg gactcgatga tcgaggaacg
     1501 agcgaaccga tgatcagccc ctccgacctc gccgctctgc gggaatcatt cacatcatgg
     1561 gaccaacccg gactaccgag ttcactcgcc actgactgcg tccgactgct agacgtcagc
     1621 gacatcacca tctcctttgt cggagcctcc ggtcagcaca tcctgtgtac ctcgtcggac
     1681 gactctcgca ccctggacga atgggagttc accctccaag aggggccatg cttcgacgcc
     1741 ggagtgaaca gcacatcgaa gaccgcagaa accgcgccct ccaacaccaa cccatggccc
     1801 cagctctcgg ccaaggccca cgccatcggc taccaatcca tcgccggtgt tcccctgcga
     1861 gctggaggaa ccaccttcgc cacactcaac ctccaagacc gacacggcaa gatcactccc
     1921 gaaactctcg ccgacgccga gcaaatcgca aaggagctgg cctcactcgt ggtcggctct
     1981 ctcagccacc agcccccgtc gccagaccag ccagtcgaac acgacacctt ccatcaagcc
     2041 accggcatgg tcatggcgca gatgggaatc gactccggat ccgccgccga cgtgctccgc
     2101 gcccacgcct gggctcacga ccgcctcctc atggacatcg cctcagaagt cgtggcccgc
     2161 acactgacat tctcgccggc ctctccaagg taagtccctt cgccgctaaa gcaacacggg
     2221 aagcctgctt tccacgaccc gaacattccg tcggggcccc gatagcgccc gagccatggc
     2281 ttgggacgct caatgcgttg gatagaaccc atctgctacc cctccgcttg cacccccgct
     2341 gcatcagaga agtgagacat accgaaagta aacccgagtg cacatttccc ctgttcagaa
     2401 acccgcttcg gtcctctcgg ctactcagaa tcttgctccg cgggtaggac tcgaacctac
     2461 aaccaatgga ttaacagtcc actgctctgc caattgagct accgcggaat ggggtggtgc
     2521 cgactggcga gcacaggtta ccaaccggac atcgctaggc cgagccgacc gcccgcagct
     2581 tgtggcacca tccggaccgt gatggacacc tcaattgccc ggtgacatcg gttggg
//
LOCUS       DASUTA010000101         4379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1071295, whole genome shotgun sequence.
ACCESSION   DASUTA010000101 DASUTA010000000
VERSION     DASUTA010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071295"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFZ80_02005"
     CDS             <1..392
                     /locus_tag="VFZ80_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019545589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6286230.1"
                     /translation="VFCRRFGVPLIDGGTVRLPRLIGQSNALDLILTGRGVDAIEAKA
                     MGLVNRVVADGEARQAAIDLAAEIASHPSTCMRNDRASAISQWSLSEEVAMAQELELG
                     LQTIGSGEASSGAARFRVGEGRHGEAL"
     gene            complement(394..2085)
                     /gene="leuA"
                     /locus_tag="VFZ80_02010"
     CDS             complement(394..2085)
                     /gene="leuA"
                     /locus_tag="VFZ80_02010"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX6286231.1"
                     /translation="MTPPIARPKKMPFEKYEPFVPLVLEDRTWPNVTIEKAPRWCSVD
                     LRDGNQALIDPMDPARKLRMFQTLIGMGFKEIEVGFPAASQPDFDFVRQLVDDDLIPD
                     DVTIQVLVQCRDELIERTYDAIEGAPRAIVHFYNSTNPLQRDVVFGMDKAGIRDIPVN
                     AAKLCRKLEESMGDTAIRYEYSPESFTLTEPDFAVEVCEAVMDEMAPTPDDPIILNLP
                     ATVEAYSPNVYADVIEWFGRSISRRDSIVLSLHPHNDRGCAVAAAEFGVMAGADRVEG
                     TLFGNGERTGNVDIITLAMNLFANGVDPELDITDIDSLRRTAEYCNRLPVHPRHPYVG
                     DLVYTAFSGSHQDAIKKGFSALPDDYDRWAVPYLPIDPKHVGRSYEAVIRVNSQSGKG
                     GVAYVMKEEHGFDLPRRLQIEFSKTIQHITEDSGTEISPAKMWESFQGVYFPEAASFR
                     LRSQELHTSTKDGSGHTTITAHLDVRGEDITVTGAGDGPVEAFVNALRHQWDVAFDVH
                     DYSEHAIGSGADAKAVAYVESSGEDGTMRWGIGVDSNITTASLLAVLAAFERQQG"
     gene            2283..3290
                     /locus_tag="VFZ80_02015"
     CDS             2283..3290
                     /locus_tag="VFZ80_02015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6286232.1"
                     /translation="MYMDFGLFLQPLHHPSEDPTEALERDLELISHLDDLGYGEAWIG
                     EHHSTGWENIAAPEAVIAAAAERTHRIRFGTGVVQLGLHHPLVALDRMILLDHLTRGR
                     VSFGMGVGGGIPSDLSVFGLTPELAGRRMQESIDVMMKLLGSSEPVTASSDWFELNEA
                     VLQLRPYSKPHMPLAVASANPKNVEFMGQVGGRVLLGGTPHLVDGMYEALERGARAVG
                     REASRDQIWLSYVLHVADSDEEAVEGFRNGAIAEFYEFQVGVNGRPEPGTSREEWYSS
                     YVDQHLIGSPDDVIEKLESIESNAGGIGGVIFMSREWAGVEAARRSWTLFAEEVMPRF
                     Q"
     gene            3611..4027
                     /locus_tag="VFZ80_02020"
     CDS             3611..4027
                     /locus_tag="VFZ80_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6286233.1"
                     /translation="MKGPSMAFLLVVLAVACGGDGASTEISAEMTDFQFTPTSWTVAA
                     GEEITIELTNNGSVGHEFVILQPGVTISSEDELPATEEELLAEFVYWEDEVEAGETKT
                     VTFTAPPEGEYQVICAIEGHFTAGMEGTLTSESPET"
     gene            4040..4312
                     /locus_tag="VFZ80_02025"
     CDS             4040..4312
                     /locus_tag="VFZ80_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5661 family protein"
                     /protein_id="HEX6286234.1"
                     /translation="MFTIQDAETALAAAGIDISEESYDLGDVRDGMNVELEHGTRFPD
                     LDVSGDDPVITLKIALAHLREFPDYYERLKIMEGEGEVARKVRADS"
BASE COUNT          828 a         1244 c         1458 g          849 t
ORIGIN      
        1 gggtcttctg ccgacgcttc ggagttccct tgatcgatgg ggggactgtg cggttgccga
       61 ggttgatcgg tcagagcaac gcattggatt tgatcctcac cgggagagga gtcgacgcca
      121 tcgaggccaa ggccatggga ctggtgaacc gtgtcgtggc cgatggggag gctcgacaag
      181 ccgccatcga tctggcagcc gagatcgctt cccaccccag cacctgcatg cgtaacgatc
      241 gagcttccgc catctcccag tggtctctgt ccgaggaggt ggcgatggct caagagctcg
      301 agctgggcct tcagaccatc gggtcgggtg aagcctcatc aggtgccgcc cgtttccggg
      361 tgggggaggg gcgccacggt gaggcgctgt gactcagccc tgctgtctct cgaatgcggc
      421 caggacggcc agtagcgacg ctgtcgttat gttcgagtcg accccaatgc cccatcgcat
      481 agtcccgtct tcgcccgagc tctccacgta ggcgacggcc ttggcgtcgg caccactgcc
      541 gatggcgtgc tcgctgtaat cgtggacatc gaacgccacg tcccactggt gtcgcagagc
      601 gttcacgaaa gcctccacgg ggccatcccc ggcaccggtg accgtgatgt cttccccgcg
      661 gacatcgagg tgggcggtga tggtcgtgtg acctgatccg tccttggtcg aggtgtggag
      721 ctcttgtgac cggagacgga acgaggctgc ctcggggaag tagacccctt ggaacgactc
      781 ccacatcttc gcagggctga tctcggttcc gctgtcctcg gtgatgtgct ggatcgtctt
      841 cgagaactcg atttggaggc gacggggtag gtcgaacccg tgttcctctt tcatcacata
      901 ggcgaccccg cccttgcccg actgcgagtt gacccggatc accgcctcgt agcttcgccc
      961 cacgtgtttg gggtcgatgg gcagataggg gacggcccac cggtcatagt cgtcagggag
     1021 agcactgaat cccttcttga tcgcatcctg atgcgaccca gagaaggcgg tgtagaccag
     1081 atcgccgacg taggggtggc gaggatggac cgggaggcgg ttgcagtatt cggcggtgcg
     1141 acggagagaa tcgatgtctg tgatgtccag ctcagggtcg acgccgttgg cgaagaggtt
     1201 catcgccagg gtgatgatgt cgacgttgcc ggttcgctcc ccatttccga acagggtccc
     1261 ttccacccgg tcggcgcccg ccatcacacc gaactcggct gctgccactg cacatccccg
     1321 atcgttatgg ggatggaggc tgagcacgat cgagtcgcgt cggctgatcg agcggccgaa
     1381 ccactcgatg acgtctgcgt acacgttggg ggagtaggcc tccaccgtgg ccggcaggtt
     1441 gaggatgatg gggtcgtccg gagtgggggc catctcgtcc atgaccgcct cacagacttc
     1501 gaccgcgaaa tcgggctcgg tgagggtaaa gctctcgggg gagtactcgt agcggatcgc
     1561 agtgtcgccc atcgactcct ccagcttgcg gcagagcttt gccgcattga ccgggatgtc
     1621 ccggatcccg gccttgtcca tgccgaagac gacgtcacgc tggagcggat tggtcgagtt
     1681 gtagaagtgg acgatggcgc ggggagcgcc ttcgatggcg tcgtacgtac gttcgatgag
     1741 ctcgtcgcga cactggacca ggacctggat ggtcacatcg tcggggatga ggtcgtcgtc
     1801 gaccagttga cggacgaaat cgaagtcagg ctggctcgcc gcggggaaac cgacctcgat
     1861 ctccttgaaa cccatgccga tcagggtctg gaacatgcgc agctttcgcg ccggatccat
     1921 cgggtcgatc agggcctgat tcccgtcccg aaggtcgaca gagcaccacc gtggtgcctt
     1981 ctcgatggtc acattcggcc atgttcggtc ttcgagcacg agaggtacga atggctcgta
     2041 cttctcgaat ggcatcttct tgggtcgggc tattggtggg gtcatctcgg aatccatagg
     2101 tcaggggtgg cacgacaacg accggggaaa agaaaaagcc cccggtctcg ggggcgggcg
     2161 aacacgagta tgtgcgcgtt cgccctacat gaggagaagg agagcgcgca gatctgtgtt
     2221 catcgagccc tcactgtagg caggggagtg tcgggaatca acggcgatga tcgtgcattg
     2281 tgatgtacat ggactttggg ctcttcctgc aaccacttca tcacccatcc gaggatccca
     2341 ccgaggctct ggagcgagat ctcgagctga tctcacacct ggacgacctc gggtacggcg
     2401 aagcctggat cggcgagcac cacagcaccg ggtgggagaa catcgcggcg ccagaggctg
     2461 tcatcgctgc agctgccgag cgcacccacc gcatccgctt cgggaccggt gtcgtccagc
     2521 tcgggttgca tcacccgttg gtggcgctcg atcgaatgat cctgctagat cacctcaccc
     2581 gcgggcgggt cagcttcggg atgggtgtcg gcggaggcat accgtccgac ctctccgtct
     2641 tcgggctgac cccggagctg gcaggaagaa ggatgcagga gagcatcgac gtgatgatga
     2701 agctgctggg ctcctcggaa ccggtgacgg cttcgagcga ctggttcgag ctgaacgagg
     2761 ccgtgctaca gctccggccg tactcgaaac cgcacatgcc tctggcggtg gcgtctgcca
     2821 atcccaagaa tgtcgagttc atgggccagg tcggcgggcg tgtgttgctc ggtggcacgc
     2881 ctcatctcgt cgacggcatg tacgaggcac tggagagagg cgcccgggcg gtggggcggg
     2941 aggcgtcgag ggaccagatc tggttgagct acgtcctcca cgtcgcggac agcgacgaag
     3001 aggcagtcga aggcttccga aacggcgcga tcgccgaatt ctacgagttc caggtaggtg
     3061 tcaacggacg cccggagccc gggacttcgc gggaagagtg gtactcgagc tacgtggatc
     3121 agcacctcat cggctcaccc gacgacgtca tcgagaaact ggagtcgatc gagtcaaacg
     3181 ccggaggcat cggcggggtc atcttcatgt cccgagaatg ggctggagtc gaagccgccc
     3241 gccggagttg gaccctgttc gccgaagagg tgatgccgcg attccagtga ttgaccagag
     3301 gagtgcggat ctcaacctct gtcgttccct aactttacat aacgtggatt atgggcgctc
     3361 ctgcaccggg gatgcagcgt ccaaaacgct caacccccaa cgagcctctc caatcgaacc
     3421 ttgcaatacg gcgggtggtc cgattcggcc tgcaggttcg atgtcagatg tctgctggcc
     3481 ccggcgcggt ggggtctttc ggccctagag gacggagacg tcgcggacta gagaagtatg
     3541 caggacccga tccgggaaag ggatcggcgt accagcgagg ctctgaagga ggcatttgac
     3601 aatgagatgg atgaagggcc cgtcgatggc gtttctgctc gtcgtgttgg cggttgcgtg
     3661 cggcggcgac ggcgcttcga ctgagatcag tgcggagatg acggatttcc agttcacccc
     3721 aacctcgtgg accgtggccg caggtgagga gataacgatc gagctcacca acaacggcag
     3781 cgtcgggcat gagttcgtga tacttcagcc cggtgtgacc atttccagcg aggacgagct
     3841 tcccgccacc gaagaggaac tactggcgga gtttgtctac tgggaggacg aggtggaagc
     3901 cggcgagacc aagacggtga cattcactgc tcccccggag ggcgagtacc aggtcatttg
     3961 cgcaatcgaa ggtcacttca ccgccggcat ggaggggaca ctcacctccg agtcgccgga
     4021 gacatagatt ggagtagaga tgttcacgat ccaggatgcc gagacggcgt tggctgctgc
     4081 cggcatcgac atatccgaag agagctatga cctgggagat gttcgcgacg gtatgaacgt
     4141 cgaactggag cacggaacca ggtttcccga tttggatgtg agcggtgacg atccggtgat
     4201 aacactgaag atcgctctgg ctcaccttcg cgagtttcct gactactacg agcgactgaa
     4261 gatcatggag ggtgaaggcg aagtcgctcg gaaggtaagg gccgattcct gattcctgtg
     4321 gtatagccag tcgatggtgc gctgagccca cccatcgact tgatgtccgc agtcttgga
//
LOCUS       DASUTA010000102         4972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_164875, whole genome shotgun sequence.
ACCESSION   DASUTA010000102 DASUTA010000000
VERSION     DASUTA010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164875"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VFZ80_02030"
     CDS             complement(<1..308)
                     /locus_tag="VFZ80_02030"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6286235.1"
                     /translation="MRVQVTLRIGLLAASRIADRAIVQPATGIEDVAVTAVASRSADR
                     AEAAASRWGLDTWYDSYEGLLSSPDIDAVYVGTPNSLHRRWTVASLASGKHVLVEKPL
                     A"
     gene            complement(293..643)
                     /locus_tag="VFZ80_02035"
     CDS             complement(293..643)
                     /locus_tag="VFZ80_02035"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX6286236.1"
                     /translation="MPTTPEVDAWFDEYEHPAKDAMLRVREIVLSDERMSETIKWKSP
                     TFMYEGNMASFNPRTKAHVSLMFHTGASIPGDHPRLEGGGDTARYMKFTDLDDVESAR
                     GEIEAVVDAWCESR"
     gene            689..2020
                     /locus_tag="VFZ80_02040"
     CDS             689..2020
                     /locus_tag="VFZ80_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX6286237.1"
                     /translation="MSDLFGARADDLAARSAPLATRMRPGSLDDVVGQPHLLGTNAAF
                     RHIVESGEPVSMLLWGPPGTGKTTLARLIANHASAAFRTLSATSAGVKDIREVLAEAR
                     QLLATEERRTVLFLDEIHRFAKNQQDALLPGVEDGTLILIGATTENPYFEVNSPLMSR
                     MTLFRLESLQPADIESLLHKATKDTERGLGELDLSLADEAATELATKTGGDARQALNA
                     LEVAARLAGAAGRDVIDADDISEALQRRLVRYDKAGDSHYDVISAFIKSVRGSDPDAA
                     MYWLMLMIEGGEDPEFIARRLVILASEDIGLADSEGLRVAVAAADALAYVGLPEATYH
                     LAHATLFLATAPKSNSVGRAIAAARALVQGGPTPSVPMHLRGSGYRGASELGHGVGYD
                     YSHDHTGGIVEQQYFPDGVDPVGIYHPGDLGDEASVKERLAEIDRVLRRKR"
     gene            complement(2040..3170)
                     /locus_tag="VFZ80_02045"
     CDS             complement(2040..3170)
                     /locus_tag="VFZ80_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6286238.1"
                     /translation="MTTDPRSRSEEKTEPWRENWPPPSYWIKVGLALIAAVVALFLLG
                     TLRGILLVILASFVLAIGFQPAIGWFEKRGMKRGLGLALVLMSFLVVFGGMLALALPL
                     IVSQAGEMLDQLPAFVETLQDGDGLIARLANLIDIERITTEGTADPEAALQFVGSAAG
                     FVFNFLTVLLVTPYFALSMPAMKRWLVRLLRPRHREDFLHLVGESTDLMANFIVGNLV
                     ISVIAGVVTWVGLTLIGVPYALALAAWVAITDLIPVLGALLGALGVAAVALMQSPETL
                     IWSMLLLIVYQQVENFLIAPRVMNRAVDLSPATVIIALMVGGSLAGLVGALLALPLAA
                     LIKIVIQDYVVQERIDQVRSEVANGDSIARRPRRKRGARPLP"
     gene            3263..3697
                     /locus_tag="VFZ80_02050"
     CDS             3263..3697
                     /locus_tag="VFZ80_02050"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6286239.1"
                     /translation="MRMSAERTISRPADEVAEFFFDASNNPKWQNGMVSCEWVSEPPI
                     RVGSTYAQLARFMGRDVRSTFVVTDFEPGRSIRIETIESTFPIQVQRRVEPIDESSSR
                     VTAEITGGPEGGLMKMFSPLMAGRAQKSVDNDYDRLVQLLES"
     gene            complement(3679..>4972)
                     /locus_tag="VFZ80_02055"
     CDS             complement(3679..>4972)
                     /locus_tag="VFZ80_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017544997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX6286240.1"
                     /translation="VPDYMGVRFKHWVDGLNGDWLISRQRFFGVAIPLWYPLDEDGRA
                     DYSNPIIPDDGQLPIDPSSHVPAGFEESQRNQPGGFIGDPDIMDTWATSSLTPQIVGH
                     WDDDPDLWNRVFPMDIRPQGPDIIRTWLFATTLRSHLEHDVLPWHTTTINGWILDPDR
                     KKMSKSKGNVVTPRQFIDDYSAEGVRYWAASGRPGTDTALDEGQFKIGRRLAIKILNA
                     AKFALGFSDEDVDPSAITEPLDMAMLAQLAETVAGATAAFEAYDYARALEIAERSFWN
                     WTDDYVELVKARAYEGGAPAESAHAALQLALSVYLRLFAPFLPFVTEEAWSWWKEGSV
                     HRASWPSADEFRGVIGEPGILDAVSSILGEVRRAKSDAKTSMRTEVELVEVTADAGTV
                     GLARQAEGDLIAAARAEAITYEDGEFAVVARLAQDSSS"
BASE COUNT          961 a         1594 c         1550 g          867 t
ORIGIN      
        1 gcgagcggct tttcgaccag gacgtgcttg ccggatgcga gggaggcgac cgtccagcga
       61 cgatgaagcg agttaggagt acctacgtag acggcgtcga tgtcaggaga tgagagcagc
      121 ccttcgtagg agtcgtacca cgtgtcaagg ccccagcgac tggcagccgc ctcggcgcgg
      181 tccgccgatc gagacgcgac ggccgtcacc gcaacatctt cgatgcctgt tgctggttgg
      241 actatcgccc ggtccgctat tcgcgacgcc gcaagtaacc cgatcctcag ggtcacctgg
      301 actcgcacca ggcgtcgacc acagcttcga tctcccctcg agcactctcg acgtcgtcga
      361 ggtcggtgaa cttcatgtat cgagcagtgt ccccgccgcc ttcgagacgg gggtggtcac
      421 ccggtatcga tgcgccggtg tggaacatga gactgacatg agccttggtg cgagggttga
      481 agctggccat gttgccttcg tacatgaagg tgggtgactt ccatttgatg gtctcgctca
      541 tccgctcatc ggagaggacg atctcacgaa cccgaagcat ggcgtccttg gccgggtgct
      601 cgtactcgtc gaaccaggcg tcgacttcgg gcgtggtcgg catgtgccgg aatctagtgc
      661 gctccaccag ggggtaccgt cgtagcagat gagcgatctc ttcggtgcac gtgccgacga
      721 ccttgccgca cgctcggccc cgctggcgac gaggatgcgg ccagggtcac tcgacgatgt
      781 cgttggccag ccacatttgc tgggcacgaa cgccgcgttc cgccacatcg tcgagtcggg
      841 tgagcccgta tccatgctgc tctggggacc gccgggcacc ggcaagacga ccctcgccag
      901 gttgatcgcc aatcacgcca gcgccgcctt caggacccta tccgcgacct cagctggggt
      961 gaaagacatc cgagaggtgc tcgccgaggc gcgccagctc ctggctaccg aggagcggcg
     1021 caccgtcctg tttctcgacg agatccaccg gttcgccaag aaccaacagg atgcacttct
     1081 ccccggagtc gaagacggga ccttgatcct catcggcgcc accaccgaga acccgtactt
     1141 cgaggtcaac agtcccctga tgagccggat gaccctcttc aggctcgaat ccctacagcc
     1201 ggccgacatc gaatcgcttc tccacaaggc aaccaaggac accgaacgcg gtctcggcga
     1261 gctcgacctc agcctcgccg acgaagccgc gaccgagctg gctacgaaga cgggcggaga
     1321 tgcccgccaa gctctcaatg ccctagaggt cgccgcccgt ctggccggtg ccgccggccg
     1381 tgatgtcatc gatgcggatg acatatcaga agcgctgcaa cgacgtctgg tgcgatatga
     1441 caaggcaggc gattcgcact acgacgtgat ctccgccttc atcaagtcgg tgcgaggctc
     1501 cgatcccgac gccgccatgt actggctgat gctcatgatc gaaggtggag aggatcccga
     1561 attcatcgca agacggctcg tcatcctggc ctccgaagac atcggcctcg ccgactccga
     1621 gggcctgcga gtcgcagtgg ccgctgccga cgcccttgcc tacgtgggtc tgcccgaggc
     1681 cacgtatcac ctggctcacg ccaccttgtt cctggcgaca gcgcctaagt cgaactcggt
     1741 cggtcgcgcc atcgccgcgg caagggccct ggtccaggga ggaccgactc catcggtgcc
     1801 gatgcatctg cggggcagcg ggtatagagg cgcttccgag ctgggacacg gagtcggata
     1861 cgactactcg cacgatcaca cgggcgggat agtcgaacag cagtacttcc ccgatggagt
     1921 cgatcccgtg ggcatctatc accccggcga cctcggagac gaagcatcgg tcaaagagcg
     1981 actggctgag atcgaccgtg tgctgagacg gaagcgatga gcgcgatcgc caggcgatct
     2041 cacggcaggg gccgcgcccc ccgctttctc ctgggcctcc gggcgatgct atccccattg
     2101 gccacctcgg atcggacctg gtcgatgcgc tcctggacga cgtagtcctg gatgacgatc
     2161 ttgatgagtg cggcgagggg tagcgccagg agcgccccca cgagcccggc cagactgccc
     2221 ccgaccatga gggcgatgat caccgtagcc gggctgaggt cgacggcccg attcatcacc
     2281 cgtggggcga tcaggaagtt ctccacctgc tgatagacga tcaagaggag catcgaccag
     2341 atcagggtct cgggcgattg catcaaggcg acagccgcca caccgagtgc gccgagcagg
     2401 gcgccgagaa ccgggatgag gtctgtgatg gccacccagg ccgccagggc caatgcataa
     2461 ggcacgccga tgagagtcag accaacccat gtgacgacgc cggcgatgac gctgatgacg
     2521 agattgccga cgatgaaatt ggccatcaag tcggtcgatt ccccaaccag atggaggaag
     2581 tcctcgcggt gtcggggacg gagcaaacgg accagccacc gtttcatcgc gggcatcgac
     2641 agtgcgaagt agggcgtgac gagtagaacg gtgaggaaat tgaagacgaa gccggcggcg
     2701 cttcccacga actggagcgc cgcttcgggg tcggcggtgc cctcggtggt gatcctctcg
     2761 atgtcgatga gattggcgag acgggcaatc agcccgtcac cgtcctgcag cgtctcgacg
     2821 aaggcaggca actgatcgag catctctcca gcctgggaga cgatcaaggg cagggccaga
     2881 gccagcatcc ctccaaacac caccaggaat gacatgagga cgagggccaa gccaagacca
     2941 cgtttcatgc ctcgtttctc gaaccaaccg atcgccggct ggaaaccgat tgcgagcacg
     3001 aaagaggcca ggatcacgag caggatcccg cggagggtcc ccaagaggaa caaggcgacc
     3061 accgccgcga tcagggcaag ccctaccttt atccagtagc tcggaggcgg ccagttctcc
     3121 cgccagggct cggtcttctc ctcggaacgc gacctgggat cggtcgtcac gaatcaccca
     3181 ccgctcgtct ccggttcagc agcgagacac agtacccgtc tccgtccaat ccaaaccgtg
     3241 attcgcctac gctgacgcca cgatgcgaat gtctgcggaa aggacgatca gtcgacccgc
     3301 cgacgaggtg gccgaattct tcttcgacgc ctcgaacaac ccgaaatggc agaacgggat
     3361 ggtctcctgc gagtgggtct ccgaaccgcc tatccgtgtc ggatccactt atgcgcagct
     3421 tgcccgattc atgggccggg atgtgaggtc gacattcgtc gtgaccgact tcgagcccgg
     3481 acgctccatc cgtatcgaga cgatcgagtc gacattcccg atccaggtgc agcgtcgtgt
     3541 ggagccgatc gacgaatcat cgagtcgcgt gactgcggag ataacaggtg ggcccgaagg
     3601 tgggctgatg aagatgttct ctcctctgat ggccggtcgc gcccagaagt cggtggacaa
     3661 cgactacgac cgtttggttc agctgctgga gtcctgagcc agcctggcga cgacggcgaa
     3721 ctcaccatcc tcgtaggtga tcgcctccgc ccgggccgcc gcgatcagat cgccttctgc
     3781 ctgtcgtgcc aatccgacag tgccggcatc ggcggtcacc tcgaccagct cgacctcggt
     3841 tcgcatcgag gtcttcgcgt ccgattttgc ccggcgcacc tcgccaagga tcgatgagac
     3901 cgcgtcgagg attccgggtt ccccgatcac acctctgaac tcgtcggccg acggccacga
     3961 ggcgcggtgg acggaccctt ccttccacca gctccatgcc tcttcggtca cgaaagggag
     4021 aaaaggggcg aacaaacgca agtacacgct cagggcgagc tgcagtgcgg cgtgagccga
     4081 ctcggctggc gcccccccct cgtaggcacg agccttgacc agctccacgt agtcatccgt
     4141 ccagttccag aacgagcgtt ccgctatctc gagagccctc gcgtagtcgt atgcctcgaa
     4201 tgccgccgtg gcacccgcca ccgtctctgc cagttgggcc agcatcgcca tgtccaacgg
     4261 ctcggtgatc gccgagggat cgacgtcctc gtctgagaac ccgagagcga acttcgccgc
     4321 gttcaagatc ttgatggcca gcctgcggcc gatcttgaat tgaccctcgt ccagggccgt
     4381 gtctgtcccc ggccgcccac tcgccgccca gtagcggaca ccctccgcgg agtagtcgtc
     4441 gatgaactga cggggcgtca ccacgttgcc cttggacttc gacatcttct tccggtcggg
     4501 gtcgagaatc catccattga tcgtcgtcgt gtgccagggc aggacgtcgt gttccagatg
     4561 ggagcgcagg gtcgttgcga agagccatgt ccggatgatg tccgggccct gaggccgtat
     4621 gtccattggg aagacccgat tccacaagtc ggggtcgtcg tcccaatgcc ccacgatctg
     4681 aggcgtcaga gacgatgtgg cccaggtgtc catgatgtcc ggatcgccga tgaagccacc
     4741 cggctggttg cgctgggact cctcgaagcc ggccggcacg tgagacgatg ggtcgatggg
     4801 gagttgtccg tcgtcgggga tgatcgggtt ggagtagtcc gcacggccgt cctcatcgag
     4861 tggataccag agaggaatgg cgacgccgaa gaacctctgc cggcttatca gccaatcgcc
     4921 gttcagaccg tcgacccaat gcttgaagcg gacacccatg taatcgggga cc
//
LOCUS       DASUTA010000103         7726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1071678, whole genome shotgun sequence.
ACCESSION   DASUTA010000103 DASUTA010000000
VERSION     DASUTA010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071678"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VFZ80_02060"
     CDS             complement(<1..368)
                     /locus_tag="VFZ80_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HEX6286241.1"
                     /translation="MERRIFGLENEYGVTCTLRGQRRLSPDEVARYLFRRVVSWGRSS
                     NVFLENGARLYLDVGSHPEYATPECDSLEDVVAHDKAGERILEGLVSGAEERLEEEGI
                     RGEIYVFKNNTDSAGNSYGC"
     gene            complement(398..1066)
                     /gene="prcA"
                     /locus_tag="VFZ80_02065"
     CDS             complement(398..1066)
                     /gene="prcA"
                     /locus_tag="VFZ80_02065"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011981501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HEX6286242.1"
                     /translation="MTIPFYVAPEQLVRDKAEFARKGIARGRSIVAVDYADGVLLLAE
                     NPSTALHKISEVYDRIAFAGVGKYNEFETLRKAGIRQADLMGYLYSRDDVTAMGLATA
                     FAQTLGMIFTRDPKPFEVEILLAEITEDGSEHRLFRVTYDGTLYDERRYVAIGGKAEQ
                     LMEALEDLWSENMTLEQAFNAGVEAFSRAEGRESEGWEVAVLDGGNGRRCFRRLSADE
                     IGTS"
     gene            complement(1063..1857)
                     /gene="prcB"
                     /locus_tag="VFZ80_02070"
     CDS             complement(1063..1857)
                     /gene="prcB"
                     /locus_tag="VFZ80_02070"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HEX6286243.1"
                     /translation="MIEKPLGPLPLDAPVPGSSFTDLLESLGAGPEITIPPGTQVPSV
                     PEGTTVLALRYEGGVVMAGDRRATEGHLVAHRSIRKVFPADRYSAVAIAGVAGLAMDM
                     VKLFQVELEHYEKIEGIRLSLEGKASFLARLVRSQLPMAIQGLVVVPLFAGYDDRETR
                     GRVYTYDVVGGRYEETEFGSTGSGSRLAKSYLRTAYKDDLGAEDATAIAVNALVAASS
                     EDTATGGPDLKRGILPNVVRIDPDGLIELDDETIYPFAETAVETIR"
     gene            complement(1854..2042)
                     /locus_tag="VFZ80_02075"
     CDS             complement(1854..2042)
                     /locus_tag="VFZ80_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006767624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HEX6286244.1"
                     /translation="MSDRDRGQAEGRRQEETEDQSTGAAETDTDLDALDDLLDEIDEV
                     LEENAEEFVKNYVQKGGQ"
     gene            complement(2056..3525)
                     /gene="dop"
                     /locus_tag="VFZ80_02080"
     CDS             complement(2056..3525)
                     /gene="dop"
                     /locus_tag="VFZ80_02080"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017241688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HEX6286245.1"
                     /translation="MAIHKVLGTETEYGIVVRGDPGFNPAVASSLVVNSYPGERVKVQ
                     WSYEEESPGRDARGFGHDTYGLIDPEGAVVNSVLANGARLYVDHAHPEYSSPESYDPL
                     EAALYDKAGEVVMHRAAMAASESAGRQISLYKNNSDGKGNSYGAHENYLLSRETPFGD
                     VIRYAMTFMVTRQVFTGAGKVGKENDRADVDFQITQRADFFEEEVGLETTLKRPIINT
                     RDEPHGDPSAYRRLHVIIGDATLSEYQTFLKMGSTALMLAALEDGAIPDPLDLFDPVE
                     ACWQVSHDVALGKPLELDGGGTATALELQGRYLEWLTKYVEKEMDDPVWDQVIAEWER
                     TLQRLETDPDSLSDTLDWVAKKRLLEGYVQRDGLQWTNNKLRALDVQYHDVDPGRGLY
                     SRLASRGSMRRLFTDEQIAEAATNPPTRTRAYFRGRCVSEFAGSLVAANWDSLVFDIG
                     EAHLKRVPMMEPLRGDASLVGTLLDEAEDAADLLRRLES"
     gene            complement(3539..5260)
                     /gene="arc"
                     /locus_tag="VFZ80_02085"
     CDS             complement(3539..5260)
                     /gene="arc"
                     /locus_tag="VFZ80_02085"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HEX6286246.1"
                     /translation="MDEGRQHEDAAQLRKVVAALEEEVSFLRRRLREAPGKAEELEAD
                     LARAQDRLDRVVSQNDKLSSLLEEAREQLGVLREEVEKLTQPPNNFGTVLKVSDDGSV
                     DILTGNRKLRVAAQPSVEVKKLQVGQEVLLNEAFNIIDVRYFEPSGEILRVREVMDDG
                     RVVVIAHADDERVLNRSEAMYDVYLRPGDYVRIDPKTSLILEKLDRPEVEELILEEVP
                     NITYSDVGGLAEQIEMIRDTVELPYLHRGLFKEYELEPPKGILLYGPPGCGKTLIAKA
                     VANSLAKKVSERTGNPDVRSYFLNIKGPELLNKWVGETERQIRLIFQRAKEKSDEGVP
                     VIVFFDEMDSLFRTRGTGISSDVESTIVPQLLSELDGVESLKNVVVIGASNREDLIDP
                     AILRPGRLDVKIKINRPNPEAAREIFKIYLSDETPLDKDEVGETPSAQAIDEMIEATV
                     ADMYSEEKDNEFLEVTYANGDRETLYFKDFASGAMIENIVRRAKKDAIKRQIAGGPGG
                     VRKNDLITAIKQEFREQEDLPNTTNPDDWAKISGKKGERIVYVRTLVGGDEDSRLIEK
                     VAGGQYL"
     gene            complement(5314..6114)
                     /locus_tag="VFZ80_02090"
     CDS             complement(5314..6114)
                     /locus_tag="VFZ80_02090"
                     /inference="COORDINATES: protein motif:HMM:NF020287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEX6286247.1"
                     /translation="MTLREGEPALLIDSKGRKFLLKLESGRTFQFHNGELSHDQLIGA
                     EDGSWFTSTGNARLLLLRPRLADFILKMKRGAQVVYPKDLGPILVYADIGPGMTVLEA
                     GTGSGALTLGLSRAVGPTGKVITVERRDDHADHARKTLTRWYGAVPDNVEMRIGDVAE
                     HVADVRPERIVLDLPEPWHTLEAAAHHQPTGGVLAAYVPTVPQVQTTVDKAAESGFAE
                     VEVMEFLMREWNISGRSVRPEHSMTGHTGFLIFMRKVADETVQGNPGN"
     gene            6237..6494
                     /locus_tag="VFZ80_02095"
     CDS             6237..6494
                     /locus_tag="VFZ80_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286248.1"
                     /translation="MTTRLINRALGYRDPRAEGVRRALRGVREGNDRELYIGLALWAF
                     AFLRDTTPKKQLLYRKVVPEGSAIVVHHKRRGDPGLEIHKP"
     gene            6728..7513
                     /locus_tag="VFZ80_02100"
     CDS             6728..7513
                     /locus_tag="VFZ80_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286249.1"
                     /translation="MRRGVWYLNVTPATWRTEVLAATLTSGRHAVASHRTAGAIYQLD
                     GIYGRILEVTVPYSNQPAPPGVILHRTRRSIAPTSFESLIVTSPARTLLDLAAFLPDR
                     VLEKAMASAFRKRLTTPDEFDSTIGRLGGRGVGGTRRARRVLRAVANDLSGSVAEVDL
                     AQLIRDAPIPSPVQQLRVELPGGRHAYPDFAWPDRMRIAEVDGLETHASQDQLASDLD
                     RQNQLLDLGWEIRRWPAQTVRREPQRVIGELTRFVLAPTPTLL"
BASE COUNT         1235 a         2454 c         2519 g         1518 t
ORIGIN      
        1 tggcacccat aggagttgcc cgcggagtcg gtgttgttct tgaacacgta gatctcaccc
       61 cggatgccct cctcctcgag ccgctcctcg gcaccggaga ccaggccctc gaggatccgc
      121 tcccccgctt tgtcgtgagc aaccacgtcc tcgagtgagt cgcattcggg tgtggcgtat
      181 tcgggatgag agccgacgtc gaggtagaga cgggccccgt tctccaagaa cacgttggat
      241 gatcgccccc aggacacaac acgacggaac aggtaccggg ctacctcgtc ggggctgagc
      301 cgacgttggc cgcgcagggt gcaggtgacc ccatactcgt tttccagccc gaagatccgt
      361 cgttccacgc ggtcaggcta gaccgcatgc ccggcttcta cgacgtcccg atttcgtcgg
      421 cggagagccg gcggaagcag cgacgcccgt taccgccgtc gaggacagcg acttcccagc
      481 cttcggactc tctcccctcg gcgcgggaga aagcctcgac gccggcgttg aaggcctgct
      541 cgagggtcat gttctcgctc cacagatcct cgagagcttc catcagctgt tctgccttgc
      601 ccccgattgc cacgtatcgc cgctcgtcgt agagggtccc gtcgtaggtc acacggaaca
      661 gacggtgctc acttccgtcc tcggtgatct cggcgaggag gatctccacc tcgaagggtt
      721 tggggtctct cgtgaagatc atgcccagtg tctgggcgaa ggccgtggcc aggcccatgg
      781 cggtgacgtc gtcgcgggag tagagatatc ccatgaggtc ggcctggcga atcccggcct
      841 tgcgaagcgt ctcgaactcg ttgtacttgc cgacgccggc gaaggcgatg cggtcgtaga
      901 cctctgagat cttgtgcaga gccgtcgatg ggttctcggc gaggagcagc acgccgtcgg
      961 cgtagtccac ggcgacgatg gaccggcccc gggcgatgcc cttgcgggcg aactcggcct
     1021 tgtctctgac cagctgttcg ggggcgacat agaaggggat ggtcatctga tggtctccac
     1081 tgccgtctcc gcgaacgggt agatggtctc gtcatcgagc tcgatcagcc cgtcggggtc
     1141 gatccggacg acgttgggga ggatcccccg cttcagatcg gggcccccgg tcgcggtgtc
     1201 ttcgctcgac gccgccacca gcgcgttgac ggcgatggcg gtcgcgtctt cggctccgag
     1261 gtcgtccttg tatgcggtgc gcagatagga cttggcgaga cgggaccccg acccggtgct
     1321 gccgaactcg gtctcctcgt agcggccacc gacgacgtcg taggtgtaca cgcgtcctct
     1381 ggtctcccga tcgtcgtatc cggcgaacag agggaccacc accaggccct ggattgccat
     1441 cggcagctgg cttcgcacca gccgggccag gaaggaggcc ttcccctcca gggagagcct
     1501 tatgccctcg atcttctcat agtgctccag ctcgacctgg aacagcttga ccatgtccat
     1561 cgccagcccg gcgacaccgg cgatggccac cgccgagtag cggtcggcgg ggaagacttt
     1621 cctgatgctt cggtgggcga cgaggtggcc ttcggtggcc cgacggtcac cggccatcac
     1681 cactccgccc tcgtagcgga gggcgagcac ggtggttccc tcgggcaccg atggcacctg
     1741 ggtccccggc gggatcgtta tctcaggtcc ggcgccgagc gattcgagaa gatcggtgaa
     1801 gctggagcct ggaactggag cgtcgagtgg cagcggtccg agtggtttct cgatcactgc
     1861 ccacccttct ggacgtagtt cttgacgaac tcctcggcgt tttcctcgag gacttcgtcg
     1921 atctcgtcca gcaggtcgtc gagtgcgtcg agatcggtgt cggtctcggc ggccccggtg
     1981 ctctggtctt cggtctcttc ctggcgccgg ccttcggcct ggcctctatc tcggtctgac
     2041 atacaagact tccttctagc tctccagacg tcgcagcagg tctgcggcgt cctcggcctc
     2101 gtcgagcaac gttcccacga gggaggcgtc accacgcaac ggctccatca taggaacccg
     2161 tttcagatgc gcttcgccga tatcgaaaac gagagagtcc cagttggctg ccaccagcga
     2221 tccggcgaac tcgctgacac aacggccccg gaagtaggcc ctggtccggg tgggtgggtt
     2281 ggtcgccgcc tcggcgatct gctcgtcggt gaagagacga cgcatcgagc cacgggaggc
     2341 gagacggctg tagagaccgc gtccggggtc gacgtcgtga tactggacgt cgagggcacg
     2401 cagcttgttg ttggtccatt gcagaccgtc tctctgaacg tagccctcga ggagcctctt
     2461 cttggccacc cagtcgagtg tgtccgacaa cgagtccggg tcggtctcca gtctctgcag
     2521 ggttcgctcc cactcggcga ttacctggtc ccacaccggg tcgtccatct ccttctcgac
     2581 gtacttggtg agccactcca gatagcgccc ctggagctcg agagccgtgg cggtgccccc
     2641 gccatcgagc tccagaggct tgcccagagc cacgtcgtgg ctgacctgcc agcaggcctc
     2701 caccgggtcg aagaggtcga gtggatcggg gatggcgccg tcctcgagcg cggcgagcat
     2761 gagcgcggtg gaacccatct tgaggaaggt ctggtactcg gagagcgtgg cgtcgccgat
     2821 gatcacgtgg agacgtcggt aggccgacgg gtcgccgtga ggctcgtcgc gagtgttgat
     2881 gatgggccgc ttcagggtgg tctccaggcc gacttcctcc tcgaagaaat cggcgcgctg
     2941 ggtgatctgg aagtcgacgt cggcccggtc gttctccttg ccgaccttgc ccgccccggt
     3001 gaacacctga cgggtgacca tgaaggtcat cgcgtagcga atgacgtcac cgaagggtgt
     3061 ctccctgctc agcaggtagt tctcgtgagc tccgtaggag ttgcccttcc cgtccgagtt
     3121 gttcttgtac agcgagatct gacgaccggc cgactccgac gccgccatag ccgctcggtg
     3181 catgactacc tcgccggcct tgtcgtacag ggcggcctcg agggggtcgt agctctcggg
     3241 agaggagtat tcggggtggg cgtggtcgac gtagagacgg gcgccgttgg ccagcaccga
     3301 gttgaccacc gcaccctcgg ggtcgatgag gccgtaggtg tcgtgaccga agcctcgagc
     3361 gtcgcggcct ggcgactcct cctcgtacga ccactggacc ttgacccgct ctccgggata
     3421 gctgttgacg acgagcgagg aggcgacggc agggttgaaa ccggggtcgc cccggacgac
     3481 tatcccgtac tcggtctccg tcccgagcac cttgtggatc gccattacag aagggccatc
     3541 agaggtactg gccgcctgcg accttctcga tgaggcggct gtcctcgtcc cccccgacca
     3601 gggtgcgaac gtagacgatg cgctcgccct tcttgcccga gatcttggcc cagtcatcgg
     3661 ggttggtcgt gttgggaaga tcttcttgct cgcggaactc ctgcttgatg gccgtgatga
     3721 ggtcgttctt cctcacaccg cccggtccgc ctgcgatctg gcgcttgatg gcgtccttct
     3781 tggcccgtct cacgatgttc tcgatcatgg caccgctggc gaagtccttg aaatagaggg
     3841 tctcccggtc accgttggca taggtcacct cgaggaactc gttgtccttc tcctcggagt
     3901 acatgtcggc gacggtggcc tcgatcatct catcgatggc ctgtgccgag ggggtctcgc
     3961 ccacctcgtc tttgtcgagc ggtgtctcgt cgctgaggta gatcttgaag atctctctgg
     4021 cggcttcggg gttgggacgg ttgatcttga tcttcacgtc cagccggccg gggcggagga
     4081 tcgccgggtc gatgagatcc tcacggttgg aggcgccgat gacgacgaca ttcttcaatg
     4141 actcgacccc gtcgagctcg ctgagcagct ggggcacgat ggtcgactcg acatcggagc
     4201 tgattccggt ccctcgcgtg cggaacagtg agtccatctc gtcgaagaac acgatgacgg
     4261 gcacgccctc gtcggacttc tccttggccc gctggaagat gagcctgatc tggcgctcgg
     4321 tctcaccgac ccacttgttg agcagctccg ggcctttgat gttgaggaag taggagcgga
     4381 cgtcgggatt accggtgcgc tcggagacct tcttggccag gctgttggcg accgccttgg
     4441 cgatgagggt cttaccgcat ccaggagggc cgtagaggag gatccccttc ggaggctcca
     4501 gctcgtactc cttgaacagg ccgcggtgga ggtaggggag ctcgacggtg tccctgatca
     4561 tctcgatctg ctcggcgaga ccgcccacat cgctgtaggt gatgttcggg acttcctcga
     4621 ggatgagctc ttcgacctcg ggccgatcga gcttctcgag gatcagcgat gtcttggggt
     4681 cgatgcggac gtagtcgccg gggcgcaggt acacgtcgta catcgcctcc gaacggttca
     4741 ggacgcgctc gtcgtcggcg tgtgcgatca ccaccacccg accgtcgtcc attacctccc
     4801 tcacccggag gatctcgccg gacggctcga agtagcggac gtcgatgatg ttgaacgcct
     4861 cgttgaggag gacctcctgg cccacctgga gcttcttcac ctcgaccgaa ggttgagcgg
     4921 cgacgcgaag cttgcggtta ccggtgagga tgtcgacgct cccgtcgtcg gagactttga
     4981 ggacggtgcc gaagttgttg ggtggctggg tgagcttctc gacctcttcg cggaggacgc
     5041 cgagttgttc tctcgcctct tcgaggagtg atgagagctt gtcgttctgg ctgacgactc
     5101 ggtcgagccg atcctgggcc cgggcgaggt ctgcctctag ctcctcggcc ttgccggggg
     5161 cctcgcgaag acgacgtcgc aggaaggaca cctcttcttc gagcgcagcc accaccttgc
     5221 ggagctgagc ggcatcctca tgttgacgtc cttcgtccac ggctacctcc tggctacctg
     5281 gtcgcctctt ggtcagtata cgaaccacac tgtttagttc cccgggttac cttgcacggt
     5341 ttcatcggcc actttgcgca tgaagatgag gaagccggtg tggccggtca tggagtgctc
     5401 gggtcggacg gagcggccgc tgatgttcca ctccctcatc aggaactcca tcacttcgac
     5461 ctcggcgaag cccgattccg ccgctttgtc cactgtggtc tgcacctgtg gcaccgttgg
     5521 cacgtaggcg gccaggacac cgccggtggg ttgatggtgg gccgctgcct ccagcgtgtg
     5581 ccagggctcg gggaggtcga ggacgattcg ctcggggcga acgtcggcca catgttcggc
     5641 gacatcgccg atgcgcatct ccacgttgtc gggcaccgct ccgtaccaac gggtgagtgt
     5701 cttgcgggcg tggtcggcgt ggtcgtcgcg acgttcgacg gtgatcacct tgcccgtcgg
     5761 gcccactgcc cgggagaggc cgagcgtgag tgcccccgat ccggtgccgg cctcgagcac
     5821 cgtcatcccg gggccgatgt cggcgtacac caggatgggc cccaggtcct tggggtagac
     5881 cacctgggcg ccacgcttca tcttgaggat gaagtcggcg aggcgagggc ggagcaagag
     5941 gagacgggcg ttgcccgtgg aggtgaacca ggagccgtct tcggcgccga tcagctggtc
     6001 gtgagagagc tcgccattgt ggaactggaa ggtccgcccg ctctccagct tgaggaggaa
     6061 ctttctccct ttgctgtcga tcaggagggc gggctcgccc tcgcgcaagg tcatgagagt
     6121 atggcgaagg cggcgacgcc catgctcagg accattccga tgaccacgat ccagatcacg
     6181 actttcgtga gttgtctgtt ctgcatagtg ttcgagacgg tagacacatg gcgaagatga
     6241 cgacgcgact gatcaaccgg gctctcggct accgcgaccc ccgcgccgaa ggtgtgcgcc
     6301 gcgccctgcg cggtgtccgt gagggcaacg accgcgagct ctacatcggc ctggccctct
     6361 gggccttcgc cttcctccgc gacaccaccc ccaagaagca gttgctctac cgcaaggtgg
     6421 tcccggaggg ttccgccatc gtggtccacc acaagcgacg cggggaccca gggctcgaga
     6481 tccacaagcc ctgaaccgtt ctgtgaacgg tggtggtcac tatttgtcgc gcgagcttca
     6541 cagaacggtg tcacgacgtc agtcttgagt cggcactcga ttcgcgcgac cttggggcat
     6601 gtgggaaacc tcggtgcggg acttcgccgc cacgcatcac ggtctcgtct ggagacgttc
     6661 cgccctcgcc ttgggggtcc cccggcacgt aatacggcac cgcgtgcgca ctgggagatg
     6721 gactgaggtg agacgcggcg tctggtatct gaatgtcacg ccggcgacct ggcggaccga
     6781 agtcctggcc gccaccctca cctcgggccg ccatgccgtg gcgtcacacc ggactgccgg
     6841 cgcgatctat cagctcgatg gcatctacgg ccgaatactc gaggtgaccg tgccgtattc
     6901 gaaccagccg gctcctcccg gggtgatcct gcatcgcacc cggaggtcga tcgcccccac
     6961 ctcgttcgaa tcgctgatcg tgaccagccc tgcgaggacc ctcctcgacc tcgctgcctt
     7021 cctgcctgac cgcgtgctgg agaaggcaat ggcgtccgcg tttcgcaaac ggctgaccac
     7081 accggatgag ttcgactcga ccatcggacg cctcggcggt cgcggggtgg gaggcactcg
     7141 gcgagcccgc cgtgtcctcc gagcggtggc taacgacctg agcggaagcg ttgcagaagt
     7201 cgatctcgct cagctgatca gagatgcgcc gatcccgtcg ccggtccagc aactgcgcgt
     7261 cgagctgccg ggtggacgcc atgcctaccc cgactttgcc tggcccgacc ggatgcgaat
     7321 cgcagaagtc gatggtctcg agacgcacgc ttcgcaggat cagctcgcaa gcgatctcga
     7381 tcgacagaac caactgttgg acctcggctg ggagatccgt cgatggccgg cacaaacagt
     7441 gcgacgagaa ccgcagagag tgatcgggga gctcacgcga ttcgtgttgg cacccacccc
     7501 gacccttctg tgaacgtggc aggtcaaagg ttgtcgcgtc ccgttcacag aacggttgtc
     7561 agacccggta gacctcgacg accgtcttgt tgcggctccc gcgtcggcgg ccgatgatga
     7621 aggcggcggc cacgacgacg cccaccgcga cggcgacgat cacggcggtg ttcctgatcg
     7681 actgggtggt gtcctcgacg gcgccgacca gttcctgcgc cttgcg
//
LOCUS       DASUTA010000104         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1071717, whole genome shotgun sequence.
ACCESSION   DASUTA010000104 DASUTA010000000
VERSION     DASUTA010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071717"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFZ80_02105"
     CDS             <1..202
                     /locus_tag="VFZ80_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009571387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX6286250.1"
                     /translation="TYLYPGLPPTPISAPGRASLEAAAAPDDTPFFFYVLSDAEGHHA
                     FAETLEEHNANVEQAREDGILP"
     gene            224..1003
                     /locus_tag="VFZ80_02110"
     CDS             224..1003
                     /locus_tag="VFZ80_02110"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286251.1"
                     /translation="MTIYRLAVLGDPVEHSRSPILHETMLDLAGLQGSYERIRADRNV
                     LSRMVAGLRKGEWHGFNVTMPLKEAAAEVADDLSPPAQRSRSVNTLLVDQGRIGGHST
                     DSTAFRALLEHDRFAHHGTILVIGSGGSAAAALAAIDPGRRVYVSARRPERAAVLTST
                     FGADLVAWGAGVAGALVINATPMGMSGESLPEGILAASRGLIDLPYGDTATPAIRWAE
                     RTGTPHVDGHEFLIRQAIESFRLWTGVEIRYSHLTDALRNV"
     gene            1058..>2179
                     /locus_tag="VFZ80_02115"
     CDS             1058..>2179
                     /locus_tag="VFZ80_02115"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX6286252.1"
                     /translation="MTLSGNLGFVPLDEILRLLTRSKQQGAVEVTGNGVRGRVFVGKG
                     GIDLATTTDDDVLHRHLVNSGYAEESTLRRVTTGETTLAALAESNHEIVELLREMTVE
                     SIYQISDQGGDFHVKEGKVSPYASPKSFDLETLLEDAQERSREWAKVSEVVPDLSAAI
                     GLRRDLGDREEVSVKSDDWKILSEIGNGKSVQEIADQLGTTEFWTARVVARLASNELV
                     ELRVVEPETEWTEPSGPIAEPSHEERSYEEPSYQSSYQEDPYETAEATAEPTEVEAEQ
                     PQEIFNSETAERDDERPAAASWWQEPQAEVEEIDEDHAPEVVSEGLSEMPSVGPVESP
                     EEAEEDTEAFLEKVFSELDSDKSDTDEGHGLLRRRRMGTL"
BASE COUNT          441 a          675 c          704 g          359 t
ORIGIN      
        1 cacctacctg tatcccggac tgccccccac gccgatctct gcgccagggc gggcgtctct
       61 cgaggcggca gccgccccgg atgacacccc gttcttcttc tatgtgctgt cagatgccga
      121 gggtcaccac gctttcgctg agacgctcga agagcacaac gccaacgtgg agcaggcgcg
      181 tgaggacggg atccttccct gactcgatgc cacaatcgac gttgtgacca tctatcggct
      241 cgccgtgctt ggcgaccccg tcgaacactc caggtctccg atcctgcatg agacgatgct
      301 cgatctagcc ggcctccagg gaagctacga gcggattcgg gcagaccgca acgtgctgag
      361 ccgcatggtg gcaggcttga ggaaagggga atggcacggt ttcaatgtca cgatgcccct
      421 caaagaggcg gcagccgagg tggcagacga cctcagcccc ccggcccagc gctcccgctc
      481 ggtcaacaca ctcctcgtcg accagggccg gatcggcgga cactccacgg actcgacggc
      541 gttcagagcc ctcctggagc acgatcgttt cgcccaccac ggcacgatcc tggtcatcgg
      601 ctcgggggga agcgcggcag cggccctggc ggcaatcgac cccggacgtc gggtttacgt
      661 ctcggcgagg cgccccgaac gggcagcagt gctgacctcg accttcggag ctgacctggt
      721 tgcctggggc gccggggtgg caggcgcact cgtgatcaac gcaactccca tggggatgag
      781 cggagagagc ctcccggagg gcatactcgc ggcctcgcgc ggcttgatcg atctgccata
      841 tggcgacaca gcgacaccgg cgattcgctg ggcagagagg acgggcaccc cccatgtgga
      901 cgggcatgag ttcttgatcc gacaggcgat cgaatcgttc cgactctgga ccggagtcga
      961 aatccggtat tcccacctga cggacgccct cagaaacgtc taaagcgctc gaagccgggg
     1021 accgattcca aaccacgagc ctttagaagg agctgaggtg acgctttcgg ggaatttggg
     1081 cttcgtgcct ttggatgaga tcttgagact gctgacacgt tccaagcagc agggtgcggt
     1141 cgaggtgacc ggcaacggcg ttcgcgggcg cgtattcgtc ggcaaaggcg ggatcgacct
     1201 cgccaccaca accgacgatg acgttcttca tcgccatctg gtcaactctg gctatgccga
     1261 ggagagcaca cttcgacgtg tcaccacagg cgagaccacg ctcgccgctc tggcagagag
     1321 caaccacgag atcgtggaat tgctgcggga gatgacggtg gagagcatct atcagatctc
     1381 cgaccagggt ggtgattttc atgtcaagga aggcaaggtt tccccatacg cctcacccaa
     1441 gtcgttcgac ctggagaccc ttctcgaaga cgcccaggag cgcagccggg agtgggcgaa
     1501 ggtcagtgag gtggtgcccg acctctcggc agccatcggc ttgcgtcgtg acctcggcga
     1561 ccgcgaagag gtctcggtca agagtgacga ttggaagatc ctcagtgaga tcgggaacgg
     1621 caagtccgtc caagagatcg ccgatcaact cggaaccacc gagttctgga ccgcacgcgt
     1681 cgtcgcccgc ctggccagca atgagctcgt cgagcttcga gtggtcgagc ccgagaccga
     1741 gtggactgag ccgagcgggc cgatagccga gccttctcac gaagagcgtt cttacgaaga
     1801 gccttcttac cagagctcgt accaagaaga cccctatgag accgccgagg cgacggcgga
     1861 gccgaccgaa gtcgaagccg agcagccgca ggagatcttc aacagcgaga ccgctgaacg
     1921 ggacgatgaa cgacctgctg cggcatcgtg gtggcaggag ccgcaggccg aggtcgagga
     1981 gatcgacgag gaccacgcgc ctgaagtggt cagcgaaggt ctttccgaga tgcccagcgt
     2041 cggcccagtc gagtctccag aagaggccga ggaggacaca gaagccttcc tcgagaaggt
     2101 gttctcggag ttggactcgg acaagtccga tactgacgag ggccacggtc tgctccggcg
     2161 tcgccggatg ggcacgttg
//
LOCUS       DASUTA010000105         4104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_554059, whole genome shotgun sequence.
ACCESSION   DASUTA010000105 DASUTA010000000
VERSION     DASUTA010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554059"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VFZ80_02120"
     CDS             complement(<1..628)
                     /locus_tag="VFZ80_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286253.1"
                     /translation="MGWVRGRLLIALGVMALVVASCGGDGSGGDEEASTPSTASDGTG
                     DTGTTSAPDQEDAEDGPTAGEGEPGERMAVITIDGESTTYDLDDITYSSIEGVDDLTF
                     ETCSPDFFGSGRFYAIGYAVDANGEILVGEDGQPAGSFTMDLPPDDWEAAERDAPGFE
                     IVLNDLDIEIATPEEAAGGTMSWALDDTTASGTAVFVNFENSYTVEFEV"
     gene            885..1223
                     /locus_tag="VFZ80_02125"
     CDS             885..1223
                     /locus_tag="VFZ80_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6286254.1"
                     /translation="MARAATTTDAFNAVAEPRRREIVELIGGCELSVNDIVEAMPISQ
                     PQVSKHLKVLKKVGLVTSRSDGRRRLYRLEAAALRPIHDWVSDFERLWNERLDRLDTY
                     LEEVQGKEHT"
     gene            1220..1729
                     /locus_tag="VFZ80_02130"
     CDS             1220..1729
                     /locus_tag="VFZ80_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6286255.1"
                     /translation="MKTSVDRTGSALVTLPSDTEIRIERKFDAPAELVFDVWTIPEHV
                     RNWWGFPEHEMTDCTIDLRVGGVYRFAAQVPDVGEVAFTGVYREIDRPRRLVATEIYE
                     AFPDTESTNTLTLDEADGVTTMVIISSYPDMSTRDAVIESGMERGLQVSLDRADQMMA
                     RLQNGDDNS"
     gene            1729..2166
                     /locus_tag="VFZ80_02135"
     CDS             1729..2166
                     /locus_tag="VFZ80_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006137011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6286256.1"
                     /translation="MTEMRLEVIVLPVTDVDRAKAFYERLGFHLDVDHQAGEGFRVVQ
                     FTPPGSDCSITFGVGLGEVTSTPVKGIHLCVADIEETVAELEDRGVEVAPIRHMGANG
                     WEDGPDPGRRKYFSYSFFKDPDDNGWVIQEVGAATSRDGGDDD"
     gene            2184..2522
                     /locus_tag="VFZ80_02140"
     CDS             2184..2522
                     /locus_tag="VFZ80_02140"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX6286257.1"
                     /translation="MRRLFHQSTHVFDIAAIGSELALPLSRDTAPRPRLVSSSTSSRM
                     ARRITVGLARMGGHGYDSSGDIFLALSTRNHLPVSGEPWRVEAIPNRQQSYRFGCVHL
                     HDGVVNGDGR"
     gene            2668..3891
                     /locus_tag="VFZ80_02145"
     CDS             2668..3891
                     /locus_tag="VFZ80_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286258.1"
                     /translation="MNAAVVGDRDGSERAAMQPHRLLPVSVWIYAAVLAVVPILVPLI
                     LRPRQIGSDFAEVVGTIVYLLVGPTYAVTGAVIVSRQPRNSVGWMLMAVATGMFVSVL
                     ADIFAPGQPSESTTAGSLVLLAVASVSWVFFIFPVFHILLTFPDGHVLSPRWRVLVWL
                     EGSMVAFMMFTALFSEEIVNVDESWSVANPIGFIPESLFDVVFEQAWDKLLLVLVIGG
                     LTSIVLRFRRSHGVERQQMKSLLYAVSFFAVVYGGAAVISGGEDSGLLDLLLPVAMVG
                     IGLAVALAVLRYRLFDIDRVMSRTVTYTVVVGLLALGVAGVSTVVGTQFRQPWVVAAT
                     TLGVAAAFNPLRRRVQTWVDRRFNRSRYDAELVMAGFGVSLRDEVDADAIANGWRDVV
                     SETMQPAMVGVWVKE"
BASE COUNT          730 a         1259 c         1270 g          845 t
ORIGIN      
        1 tgacttcgaa ctcgaccgtg tagctgttct cgaagttgac gaacaccgcg gttcctgacg
       61 cagtggtgtc atcgagagcc caagacatcg tccccccggc tgcctcctcc ggagtggcga
      121 tctcgatgtc gagatcgttg agcacgattt cgaatcctgg cgcatcacgc tcagcggcct
      181 cccaatcatc cggtgggaga tccatcgtga agctgcccgc aggctgaccg tcctctccaa
      241 ccaggatctc tccgttggca tcgaccgcat agccgatggc atagaagcgg cccgatccga
      301 agaagtccgg tgaacaggtc tcaaaggtca agtcgtccac gccttcgatc gacgagtacg
      361 tgatgtcgtc gaggtcgtag gtcgtggact cgccgtcgat ggtgatcacg gccatacgct
      421 cccctggctc accttcccct gccgtcggcc cgtcttcggc gtcttcctga tctggcgccg
      481 aagtggtccc ggtatcacct gtcccgtccg aggctgtcga tggagtggag gcttcctcat
      541 cacctccgct gccatcgcca ccgcacgaag ccaccacgag cgccatcact cccagcgcta
      601 tcaacaatcg accacgtacc caacccattc gctcactccc ttgcactcgg gcctacgcca
      661 tgccaatgga cacgccgcaa cgggcgccgt tcccggcggc ccgacgcatc tggagaggaa
      721 tggcccctgg acctgatacg agccggacaa ccctctcccg ccctggaacg aacataagga
      781 ggaagcgggg gagtcgggat actcaatgtc gaagggacca ctactcattt gcgaagtcgt
      841 cggcaggatt gacatattct tatataggaa tatatattgg ctctatagcc agagccgcca
      901 ccaccaccga tgccttcaac gccgtggccg agccgcgacg tcgggagatc gtcgagctga
      961 tcggtggctg tgagctctcg gtcaacgaca tcgtcgaggc gatgcccatc tcccagcccc
     1021 aggtctccaa gcatctcaaa gtgctcaaga aggtcgggct ggtgacatcg cggagcgatg
     1081 gccgccgtcg gctctatcgg ctcgaggccg ctgcgcttcg ccccattcac gactgggtga
     1141 gcgacttcga gagactctgg aacgagcgcc tcgaccgtct cgacacctac ctcgaagagg
     1201 tccaaggaaa ggaacacacg tgaagacatc agtcgaccgc accggttcgg cgctagtcac
     1261 gctgccgtcg gacaccgaaa tcagaatcga gcgcaagttc gatgctcctg cagagctcgt
     1321 cttcgatgtg tggacgattc cggaacacgt ccgcaactgg tggggctttc ccgagcacga
     1381 gatgaccgac tgcactatcg acctgcgcgt cggtggggtc tatcgattcg ccgcgcaagt
     1441 tcccgacgtc ggcgaggttg ctttcaccgg ggtctacaga gagatcgatc ggccccgtcg
     1501 tctggtggcg acagagatct acgaggcatt ccccgacacc gagtcgacca acactctgac
     1561 cctcgatgag gcggatggtg tgaccacgat ggtcatcatc tcctcgtacc ccgatatgtc
     1621 gaccagagat gccgtcatcg agtcgggtat ggagcgtggg ctccaggtga gcctggaccg
     1681 agcagaccag atgatggccc gccttcagaa cggagatgac aactcctgat gaccgagatg
     1741 aggctggagg tcatcgtcct gcccgtcacc gatgtcgatc gggccaaagc attctacgag
     1801 cggctcggct tccacctcga cgtggaccat caggcgggag agggcttccg ggtggtgcaa
     1861 ttcactccgc cgggctccga ctgctccatc acctttgggg tcgggctggg cgaggtcacg
     1921 tcgacaccgg tcaagggcat ccacctctgt gttgcagata tcgaggagac cgtggccgag
     1981 ctggaggatc ggggtgtcga ggttgcaccg atccgccaca tgggcgccaa tggttgggag
     2041 gacggtcccg acccgggccg ccgaaagtat ttcagctact ccttcttcaa agatcccgat
     2101 gacaacggct gggtgattca ggaggtcggt gccgccacct cacgtgatgg aggagacgac
     2161 gactagacca ccggggccta gacgtgcgtc gactcttcca ccagtcgacg catgttttcg
     2221 atatcgccgc aattggaagc gagctggcgc tgccgctctc cagagacacc gcaccgagac
     2281 cccgcctcgt ctcctcatcc acatcctccc gcatggctcg gaggatcacc gtcggcctgg
     2341 cccggatggg cggacacggg tatgactcca gcggtgacat cttcttggcc ctttccacca
     2401 ggaaccacct cccggtatcc ggggagccct ggcgggtgga ggcgatcccc aaccgccagc
     2461 agagctatcg ctttgggtgt gtccacctcc acgatggcgt cgtcaacggt gacggtcggt
     2521 gagtcgagca gcctgatatc gagcatgtcc taccttccct ggccaagaaa gcccgttacg
     2581 agaaagtgat ctcctcgata tcgtgacgat ggggcgctgt tttctctccg tagcgtttgc
     2641 gtaacgtcgc tgagggatga tgacgagatg aacgcggctg ttgtgggcga tcgcgatgga
     2701 agcgagcggg ctgcgatgca gccccacagg ctcttgccag tctctgtgtg gatctatgcg
     2761 gccgtcctgg ctgtagttcc gatcctggta cctctcatcc tcagaccccg gcagatcgga
     2821 tctgatttcg ccgaggtcgt gggcacgatc gtctatctcc tcgtcgggcc aacgtacgcc
     2881 gtcactgggg ctgtgatcgt ttcgagacag ccgagaaact cggtgggctg gatgctgatg
     2941 gcggtggcca cggggatgtt cgtctctgtc ctcgccgaca tctttgcccc gggccagcct
     3001 tcagagtcga ccacggccgg gtcactggtc ctgctggcag tggcctcggt ctcatgggtg
     3061 ttcttcatct tccctgtctt ccacatcctc ctcacctttc ccgacgggca cgtgttgtcg
     3121 ccacgatggc gagttctcgt ttggctcgaa gggtcgatgg tggcgttcat gatgttcacc
     3181 gcgttgttca gcgaggagat cgtcaacgtt gacgagagtt ggtcggtggc gaacccgatc
     3241 gggttcatac cggagtcgct gttcgacgtc gtcttcgagc aagcctggga caagctcctc
     3301 ctggtcctcg tcatcggggg actcacctcg atagtgcttc gtttccggcg ctcgcacgga
     3361 gtggaacgcc agcagatgaa gtcgcttctg tatgcggtgt ccttcttcgc ggtggtctac
     3421 gggggagccg ccgtcatctc cggtggggag gattcggggc tcctcgacct gctgttgccg
     3481 gtggcgatgg tcggaatcgg tctggcggtg gccctggcgg tcctccgcta tcggttgttc
     3541 gatatcgatc gggtcatgtc acgcacggtc acctacaccg tggtggtcgg tttgctggcc
     3601 ctcggagtcg cgggtgtctc cactgtggtg ggcacccagt ttcgtcagcc ttgggtggtg
     3661 gcggcaacga cgctgggcgt tgccgcagcg ttcaacccgt tacggcgtcg ggtgcagacc
     3721 tgggtcgacc gtcgtttcaa tcgctcccga tatgacgccg agttggttat ggcggggttc
     3781 ggtgtctcgt tgcgcgacga ggtggatgca gacgccatcg ccaacggttg gcgcgatgtg
     3841 gtctcggaga cgatgcagcc ggccatggtt ggcgtgtggg tgaaggagtg aggctctggc
     3901 cgaacggcgt gggttaccag aagggctgag agttcgccga gcgccgcagc gcgctctgta
     3961 tccacacgtc atatctcgcg catgagctca tcgaagcgcc ggtggtcggc aagactcaga
     4021 tcgactacgt cgctgtccaa ggtgaacctc cggctttcac ttacgaacgg gttaccccgg
     4081 cggcgagctg acgtaaactg gaga
//
LOCUS       DASUTA010000106         3778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_682921, whole genome shotgun sequence.
ACCESSION   DASUTA010000106 DASUTA010000000
VERSION     DASUTA010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682921"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VFZ80_02150"
     CDS             <1..349
                     /locus_tag="VFZ80_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX6286259.1"
                     /translation="DRRQLVTNHEALGYFADRYDFEIVGVVIPGGSTLGDPSSAELAE
                     LVETIERNDVDVIFTETSEPGSLAEAVAAEAGREVAVVVLFTESLGEEGADAGTLTGM
                     LLENARLIAEALG"
     gene            362..1198
                     /gene="aztB"
                     /locus_tag="VFZ80_02155"
     CDS             362..1198
                     /gene="aztB"
                     /locus_tag="VFZ80_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018024549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease AztB"
                     /protein_id="HEX6286260.1"
                     /translation="MDWLLEPFQLAFQQRALVAGSLAAIALALVGTWVVIRGMSFLGD
                     ALVHGVVPGIAVAILFDFNVLLGAIAAAGVMLAGINLVHRQTTFSEDTSIGLLFVGML
                     GLGVILISRTDSFTGSLTAILFGDALGVTTEDIALLSVLLAVTVLATLALYRPFLALS
                     FNEQKAQLLGLRPRLAHVALLVLITLTIVGSFQTVGTLLVFGLLVGPAATAALLVHRV
                     PAMMLVAAAVGVVSVALGLFISYHADTSGSATMAVVPIAFFFLTLTAKSITRKRLATA
                     PG"
     gene            complement(1203..2108)
                     /locus_tag="VFZ80_02160"
     CDS             complement(1203..2108)
                     /locus_tag="VFZ80_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX6286261.1"
                     /translation="MRISLRYVTHFRYGAPVWESHNSLRACPLDGNGQRLLDYRLFIT
                     PEVPVFTYTDNWGTRVDIFDVPQPHSELVVTAEADVDTTAPALPMSGPLAGTADMTYI
                     EDNWLYLQPTRHTRWAEEIKSAARAEMRASTDVVEIVNAIDESVRLNVEYRSGATEIG
                     FDPARIWREKVGVCQDFAHLTIAMLRSVGIASRYVSGYFYASDPTASSSDETDEIVVA
                     THAWVEIAVPEWGWWAIDPTNGSPVGERHIKIGHGRDYDDVTPLRGVYYGESDHDLAA
                     EVTMSTGRIVNVSVPEVSLSAMAEQ"
     gene            complement(2109..3065)
                     /locus_tag="VFZ80_02165"
     CDS             complement(2109..3065)
                     /locus_tag="VFZ80_02165"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEX6286262.1"
                     /translation="MLARHAENLFWIGRYVERAEDSARLLDVTYHGLLEAGSERTPAD
                     VWSECLEMLMLEDEGIEEIAGDAIGERLIADRTEPGSLVAIVAKARENARTTRELLSS
                     EVFEAMNDLHLYLGNTDLVAAARDRPYEVLRTVKTSCQAVNGSVDASMPRGEGYWFFL
                     GGQRLERALISSRVLRVWHRRLGRLASQAAFSEWVRLLRSVSAYEAYLRTYRAAMDEG
                     QVVAFLLQSEDFPRSVLHCLRMVENLLDEIDTGEVGAGARREAGRLRSSVEFVDLAAL
                     DQQRLESFLETVETGVLDLAGSLEKAYFRPAGPIFMHSYEAF"
     gene            complement(3065..>3778)
                     /locus_tag="VFZ80_02170"
     CDS             complement(3065..>3778)
                     /locus_tag="VFZ80_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX6286263.1"
                     /translation="ARLMGVELVEGRDLVVDGRVVYSRTTQGLARVDVIYRRVGDEFL
                     DPVVFRPDSLLGVPGIMSAVRAGNIALANPVGNGAADDKGVYPYVPRMIQYYLGEEAI
                     LPNVQTYLPWEPDEMDYVVENVDRLVVKPVAEAGGKGIVFGPSSDEKELESVIAAIRA
                     HPRGFIAQEVIQLSTHPTFTEKGIAPRHIDLRPFVLSGADGITVVPGGLTRVALREGS
                     LVVNSSQGGGSKDTWVLTD"
BASE COUNT          647 a         1248 c         1151 g          732 t
ORIGIN      
        1 tgaccgtcgc cagctcgtca ccaatcacga ggcgttgggc tatttcgccg accgttatga
       61 cttcgagatc gtcggagtgg tcattcctgg agggtccact ctcggcgacc ccagctcggc
      121 cgagctggcc gaattggtgg agaccatcga acgcaacgac gtcgatgtca tcttcaccga
      181 gacctcagaa ccgggaagtc tcgccgaggc ggtcgccgcc gaagcaggta gggaggtcgc
      241 cgttgtcgtt ttgttcacgg agtcgttggg cgaggagggc gccgacgccg ggaccctgac
      301 cgggatgctg ctcgaaaacg ctcggctcat cgccgaagcc ctgggctgat accttctcct
      361 cgtggactgg ctactcgaac cctttcagct ggccttccag caacgggctc tcgtcgccgg
      421 ctcgctggca gccatcgccc tcgctctggt aggcacttgg gtggtgatcc gcgggatgtc
      481 gttcctcggc gatgccctgg ttcacggggt cgttcccgga atcgccgtcg ccatcctgtt
      541 cgacttcaac gtgctcctcg gtgcgatcgc cgcagccgga gtcatgttgg ccgggatcaa
      601 cctcgtgcac cgtcagacca ccttctccga ggacacctcc atcgggctcc tgttcgttgg
      661 catgctgggg ctgggggtca tcctcatatc gcgtaccgac tcgttcacgg gcagcctcac
      721 cgccattctc tttggcgacg ctttgggggt gaccacagag gacatcgccc tactctcggt
      781 cctgctcgcg gtgactgtgc tggcaactct ggccctctat cgccccttcc tcgctctctc
      841 gttcaacgag cagaaggcgc aactactcgg actgcggcca aggctggccc acgtcgcctt
      901 gctggttctc ataaccctga ccatcgttgg ctcgttccag acggtgggca cattgctcgt
      961 cttcggtctt ctggttggac cggccgccac cgccgcgttg ttggtgcaca gggtccctgc
     1021 gatgatgctg gttgccgcag cggtaggagt ggtcagtgtg gcgctcggac tcttcatcag
     1081 ctaccacgcg gacacttcgg gatccgcgac gatggccgtg gtcccaatcg ccttcttctt
     1141 cctgacgctg accgccaaga gcatcaccag gaagagactg gccacagcgc ccggatagcc
     1201 ggctactgct cggccatggc cgagagactg acctccggca cagatacgtt gacgattcgt
     1261 cccgtcgaca tggtgacctc ggcggccagg tcgtggtcac tctccccgta atagacaccg
     1321 cgcaatggtg tgacgtcgtc gtagtcgcgc ccgtggccga tcttgatatg cctttccccc
     1381 accggggagc cgttcgtggg gtcgatcgcc caccagcccc actctggaac ggcgatttcg
     1441 acccaggcat gtgtggccac cacgatttcg tcggtttcgt cgctcgagct ggcggtgggg
     1501 tcggatgcgt agaagtatcc cgacacatat cgggatgcga tgcccaccga gcgcaacatc
     1561 gcgatcgtca gatgtgcgaa gtcctggcac acgccgacct tctccctcca gatcctggcc
     1621 ggatcgaaac cgatttccgt ggctccactc cggtactcga cgttcagtcg caccgactca
     1681 tcgatggcat ttacgatctc gacgacatcg gtggaggctc gcatctcggc acgggcggcc
     1741 gacttgatct cctcggccca gcgcgtgtgt ctggtcggct gaaggtagag ccagttgtcc
     1801 tcgatatagg tcatgtcggc tgttccggca agcggtcccg acatcggtag agccggggcg
     1861 gtggtgtcga cgtcggcttc cgcggtcacc accaactcgc tgtggggctg gggcacgtcg
     1921 aagatgtcga cccgggtgcc ccagttgtcc gtgtatgtga aaacgggcac ctcgggggtg
     1981 atgaagagcc ggtaatcgag gagtctctgc ccgtttccat cgaggggaca ggcacgaagt
     2041 gagttgtgcg actcccaaac gggtgcacca tacctgaagt gtgtcacgta gcgcagcgag
     2101 atcctcacct agaaggcctc atagctgtgc atgaagatcg gcccggcggg ccggaagtac
     2161 gccttttcca aagagcctgc caggtcgaga accccggtct cgacggtctc caggaatgac
     2221 tcgagacgtt gttggtcaag agcggccaga tcgacgaatt cgacgctgct ccgcagacgc
     2281 cctgcctcac gtctggcccc ggcccccacc tcaccagtgt cgatctcatc gaggagattc
     2341 tccaccatgc ggaggcaatg gaggacgctt ctcgggaaat cttccgattg cagcaggaac
     2401 gccaccacct gtccctcgtc catggctgcc cgataggtgc ggaggtaggc ctcgtatgcc
     2461 gacaccgagc gcaggagccg gacccactcg ctgaacgccg cctgggaggc gagccggcca
     2521 agcctgcgat gccacacccg gagcactcgc gatgagatga gagcccgctc gagacgctgt
     2581 ccacccagga aaaaccagta gccctcccct cgtggcatcg acgcgtccac cgagccgttg
     2641 accgcctgac acgacgtctt gaccgttcga agcacctcgt acgggcgatc gcgcgcagct
     2701 gccaccagat cggtgttgcc caggtagagg tggaggtcgt tcatggcctc gaacacctcg
     2761 ctcgacagga gctcacgcgt ggtgcgggcg ttctccctgg ccttggcaac gattgcgacc
     2821 agcgagcccg gctccgtgcg gtcggcgatg agcctctccc cgatggcgtc gcctgcgatc
     2881 tcctcgatgc cctcgtcctc gagcatcagc atctcgagac actcggacca cacatcggcg
     2941 ggtgtccgct ccgacccggc ttcgagcagc ccgtggtacg tcacgtcgag tagccgggcg
     3001 ctgtcttcgg cccgctccac atagcggcct atccagaaga gattctcagc gtgtcgggcc
     3061 agcatcagtc tgtcagcacc caggtgtcct tcgacccccc gccctgggac gagttgacga
     3121 ccagcgagcc ttctctcaac gcaaccctgg tgagacctcc cggcaccacc gtgatgccgt
     3181 ccgcaccgga gagcacgaat ggtcgcaagt cgatatgacg cggcgcgatg cccttttcgg
     3241 tgaatgtcgg gtgtgtggac agctggatca cctcttgtgc tatgaagcct ctcgggtgag
     3301 cacgaatcgc cgcaatcacc gattcgagct ccttctcgtc ggacgagggg ccgaagacga
     3361 tccccttccc gcccgcctcg gccacgggct tgaccaccag acgatccacg ttctcgacca
     3421 cgtagtccat ctcgtcgggc tcccatggca ggtacgtctg cacattgggg aggatcgcct
     3481 cctcaccgag gtagtactgg atcatgcggg gaacgtaggg atagacgcct ttgtcgtcgg
     3541 cggcgccgtt acccacgggg ttggcgaggg cgatgtttcc cgcccggacc gctgacatga
     3601 tgccgggaac cccgagcaga gagtcgggcc ggaacaccac cgggtcgagg aactcgtcgc
     3661 ccacccgcct gtagatgacg tcgacgcggg caagaccctg ggttgtcctg gagtagacaa
     3721 cccggccgtc gaccacgagg tcgcggcctt cgaccaactc gactcccatc aaacgggc
//
LOCUS       DASUTA010000107         4610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1331560, whole genome shotgun sequence.
ACCESSION   DASUTA010000107 DASUTA010000000
VERSION     DASUTA010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331560"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VFZ80_02175"
     CDS             complement(<1..1041)
                     /locus_tag="VFZ80_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX6286264.1"
                     /translation="MSNPEVNSPPAHLDQREKWLVLVAVAASTLLATIDGSIVNVAFP
                     TLVDELETSFNVIQWVALAYLLTIATLTMSMGRLGDVVGKKRLFVTGIAIFTVGSALC
                     GLAPDVSWLIGFRVLQAIGSVLVLALGAAILVEAFPPHERGKALGWIGTTVSLGVVSG
                     PVIGGILISAFDWRAIFFVNIPVGIAAIYLSMRHVPDRPPVRGQRFDIGGAVLMSVAL
                     FSLSLALTLGQDLGFGSTLVITGFAVATLAAAAFVVVELRIESPMLQLRLVRNPMLSV
                     SVTTGFLMFSCVSATFFLLPFYLEGVLGFEVGQVGLLLGVAPLMMGLVSPISGSLSDR
                     FGVRRLTLLGLVL"
     gene            complement(1085..1732)
                     /locus_tag="VFZ80_02180"
     CDS             complement(1085..1732)
                     /locus_tag="VFZ80_02180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX6286265.1"
                     /translation="MPMTPERWNYIGEYANEVFGQSDDHLAGLMDRAVDAGLPAIAVS
                     ADVGRLLKILVSLTDGRVAIELGTLGGYSGIWIARGLAADGKLYTVEYADDHADFAQQ
                     EFERAGVAHKVEIVRGPALDELPGLYHRLGDSSVDFAFIDAVKSQYVEYFKLLKPMMK
                     VGSLLVADNVYATGAGWIDEGHGTDGFNRLVAGDAEFEAVATPMREGILIARRTG"
     gene            complement(1747..2151)
                     /gene="arfB"
                     /locus_tag="VFZ80_02185"
     CDS             complement(1747..2151)
                     /gene="arfB"
                     /locus_tag="VFZ80_02185"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011936781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX6286266.1"
                     /translation="MDDLTIGEHTIPDAELEESFETSGGPGGQHANRNETAVRLRFHI
                     PGSSLPEDARRRLIQRYGETIEVRVSESRSQFRNRALARQHLKEKLEEGLKKTPRRRK
                     TRPSRASKRRRVAEKRARGEIKKLRRDPPIED"
     gene            complement(2249..2629)
                     /locus_tag="VFZ80_02190"
     CDS             complement(2249..2629)
                     /locus_tag="VFZ80_02190"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6286267.1"
                     /translation="MGDHPNLAIGKKLMDAFNSGDMEAGSALFSEDVVWHTIGGPTLH
                     GLDQLAASMSGREDFTIETEVHDMLANDDHLIALVEATAKAGEQEFNYRTAEIMHVED
                     GKITERWAFSDDTTAINEFFGQFS"
     gene            complement(2777..4099)
                     /locus_tag="VFZ80_02195"
     CDS             complement(2777..4099)
                     /locus_tag="VFZ80_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX6286268.1"
                     /translation="MKQAENIAPAEGKLGVLTPGMGAVATTFVAGVLAARKGYAQPTG
                     SLTQLAHIRLGKRDEDRNPLIKDFVPLAGLEDLEFGGWDPYSDDAYAAAMKAGVLDPR
                     HIEGIGDELRAIRPMSAVFSPRWVKNLHNTDQVKSEASHMDHAERLMEDIDRFRAEKK
                     VDRVVAVWCGSTEAYEEPSAVHDDLDSFEQGLKDNDEAITPSQVYAYACLKMGVPFAN
                     GAPNLTVDIPAMVELASRDGVPIAGKDFKTGQTLMKTMIAPGLKSRMLGINGWFSTNI
                     LGNRDGEVLDDPESFRSKEVSKLGVLEHILQPDLYPDLYGDIYHKVRINYYPPKGDDK
                     EGWDHIDIFGWLGYPMQIKVDFLCKDSILAAPIVLDLALFMDLAARAGRSGIQEWLSF
                     YFKSPQHAESVYPENDLFIQLTKLKNNLRHMMGEDLITHLGTEYYDEA"
     gene            4175..>4610
                     /locus_tag="VFZ80_02200"
     CDS             4175..>4610
                     /locus_tag="VFZ80_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286269.1"
                     /translation="MDSTPDGLGPTVDVALLADSVQAVRGKLFVLGGGWDTLWVPRLP
                     ARHPSLAIGLRLRIPVSWKSESLKLSVELQDADGQALLPRPLSHDIKLPTNAQKPAGA
                     TDFGLVRSFTFNNLRFDSEGAYSFVISVDDQPVSRVRFSVRMR"
BASE COUNT          826 a         1452 c         1435 g          897 t
ORIGIN      
        1 caggaccaag ccgagcaggg tgagacgtcg cacgccgaag cggtctgaaa ggcttcccga
       61 gatcggtgag accagcccca tcatcagcgg agccactccc aggagaagcc ccacctgacc
      121 gacctcgaag ccgaggacac cctcgaggta gaacggcagc aagaagaagg tcgcagacac
      181 gcagctgaac atgagaaagc cggtcgtgac gctcaccgac agcatggggt tccggacgag
      241 acgcagctgt agcatcggcg actcgatcct cagctcgacg acgacaaagg cggctgctgc
      301 cagcgtcgca acagcgaagc ctgtgatcac gagcgtcgac ccaaatccga ggtcctgacc
      361 cagagtgagg gccagggaca ggctgaacaa ggcgacgctc atcagcaccg cacccccgat
      421 atcgaagcgc tgtcctcgca ccggtggccg atcgggaaca tgacgcatcg acagatagat
      481 ggccgcgatg cccaccggga tgttgacgaa gaagatggca cgccagtcga aggcagagat
      541 gaggatcccg ccgatgacgg ggccgctgac gacccctagt gagaccgtag tccctatcca
      601 tccgagggct ttgccccgct catggggcgg gaatgcctcg acgaggatgg cggcacccag
      661 cgcgaggacc agcaccgacc cgatcgcctg gagcacgcgg aacccgatca gccacgacac
      721 gtcgggcgcc agaccgcaca gggcagaccc gacggtgaag atagcgatcc cggtcacgaa
      781 gagccgcttc ttgcctacga cgtcgcccag ccgccccatg ctcatggtga gcgtggcgat
      841 ggtaagcagg taggccagcg ccacccactg gatcacgttg aacgacgttt ccagctcgtc
      901 gaccagagtg ggaaaagcga cgttgacgat cgacccgtcg atggtcgcca gcagcgtgct
      961 ggcggcgacg gcgacgagga cgagccactt ctcgcgctga tcgaggtggg caggcgggga
     1021 attgacctcg gggttgctca actgaccttg caactgcccc accgggagcg atattccagc
     1081 acgctcagcc ggttcgacga gcgatgagga tgccttcacg catgggagtg gcgaccgcct
     1141 cgaactcggc gtcaccggcg acgaggcggt tgaacccatc tgtgccgtga ccctcgtcga
     1201 tccatccggc gccggtggcg tagacattgt cggccaccag caggctcccc accttcatca
     1261 tcggcttcag cagcttgaag tactcgacgt actgcgactt gacggcgtcg atgaaggcga
     1321 agtcgaccga gctgtcgccg agtctgtggt agaggccggg cagctcatcg agtgcgggtc
     1381 cacgcacgat ctcgaccttg tgggccactc cggctctctc gaattcctgc tgggcaaaat
     1441 cggcatgatc gtcggcgtac tccaccgtgt acagcttgcc gtctgcggcg agcccacggg
     1501 cgatccagat ccccgagtac ccgccaaggg tccccaactc gatcgccaca cggccgtcgg
     1561 tgagggacac gaggatcttg aggagccggc cgacgtcggc ggagaccgcg atcgctggga
     1621 gtccggcgtc gacggcacga tccatcaatc ctgcgagatg atcgtcggac tgaccgaata
     1681 cttcgttggc gtactcgcct atgtagttcc agcgctcagg ggtcatcggc atgggaggag
     1741 gctatctcag tcctcgatgg gcggatctcg acgcagcttc ttgatctcgc cacgagcccg
     1801 cttctcggcg actctcctcc ttttcgacgc ccggcttggc cgggtcttgc gccgccgtgg
     1861 agtcttcttc agaccctcct ccagcttctc cttgagatgt tgacgtgcca acgcccggtt
     1921 gcggaactgt gaacgcgact ctgagaccct cacctcgatc gtctcgccgt agcgctggat
     1981 gagtctccgg cgcgcgtcct cgggtagcga cgagcccggg atgtggaacc tcagccgcac
     2041 cgccgtctcg tttcggttgg cgtgctgacc accgggaccg ccggacgtct cgaagctctc
     2101 ctcgagctcg gcatctggga tcgtgtgctc gccgatggtg agatcgtcca tgtcaccgag
     2161 ggtagagggg accagggtgc cctggaggag cgtgaggttc cccctggctc gccagggccc
     2221 tggccgcggt tggccgatgg ccgtcttgct agctgaactg tccgaagaac tcgttgatcg
     2281 ctgttgtgtc gtctgagaac gcccagcgct cggtgatctt cccgtcctcc acgtgcatga
     2341 tctccgcggt ccggtagttg aactcctgtt cacccgcctt tgcggtggct tcaaccagcg
     2401 cgatgagatg gtcgtcgttg gccagcatgt cgtggacttc ggtctcgatc gtgaagtctt
     2461 cgcggccgct catgctcgcc gccagttgat ccagcccgtg aagtgtcggc ccaccgatgg
     2521 tgtgccacac gacgtcttca gagaagagtg cgctgcccgc ttccatgtcg ccggagttga
     2581 atgcgtccat cagcttcttc ccgatggcga ggttggggtg gtctcccatg tctcactcct
     2641 ttgtacgaga cggcgcaaac ctacaccgcc ctgatcggcg ccactgattg cagccctcaa
     2701 cgcgaatggc gggatgtgaa gcacctcgtt catcgaactt gcagttccat ctcatgaaac
     2761 tgcaaacgtg atttcttcag gcctcgtcgt agtactcggt cccgaggtgt gtgatcaggt
     2821 cttcacccat catgtggcgc aggttgttct tcagcttggt gagctgaatg aacaggtcgt
     2881 tctccgggta gacgctctcg gcgtgctgcg ggctcttgaa gtagaaggac agccattcct
     2941 ggatgcccga ccttcccgct ctggcggcca ggtccatgaa caacgccagg tcgaggacga
     3001 tcggagcggc gaggatcgag tccttgcaga ggaagtcgac cttgatctgc atcgggtagc
     3061 ccagccagcc gaagatgtcg atgtgatccc agccttcctt gtcatcgcct ttgggtgggt
     3121 agtagttgat cctgaccttg tgatagatgt ctccgtagag gtcggggtag aggtcgggct
     3181 ggaggatgtg ctcgaggacc ccgagcttgg acacttcttt ggagcggaag ctctccggat
     3241 cgtcgaggac ctcgccatcg cgattcccca agatgttggt cgagaaccag ccgtttatgc
     3301 ccagcattct ggacttcaga ccaggggcga tcatcgtctt catgagtgtc tggccggtct
     3361 tgaagtcctt gcccgctatg ggcacgccgt ccctcgaggc gagctcgacc atggccggga
     3421 tgtcgaccgt gaggttggga gcgccgttgg cgaatgggac gcccatcttg aggcatgcat
     3481 atgcgtatac ctgtgaaggt gtgatcgcct cgtcgttgtc cttgaggccc tgctcgaagc
     3541 tgtcgaggtc gtcatgaact gcgctgggtt cctcataggc ctccgtggat ccacaccaga
     3601 cggcaaccac ccggtcgacc ttcttctcgg cgcggaaccg atcgatgtct tccatgaggc
     3661 gctcggcgtg gtccatgtgg ctcgcttcgc tcttgacctg atcggtgttg tggagattct
     3721 tcacccagcg cggtgagaac acggccgaca tcggcctgat cgcccgcaat tcgtctccga
     3781 tgccctcgat gtggcggggg tcgaggacgc cggccttcat cgccgcggcg taggcgtcgt
     3841 ctgagtaggg atcccaacca ccgaactcga gatcctcgag cccggcgaga ggcacgaagt
     3901 ccttgatcag cggattgcga tcctcgtctc gtttcccgag acgaatgtga gccagctgtg
     3961 tgagagagcc ggtcggttgt gcatacccct tgcgagctgc gaggactccg gccacgaagg
     4021 tcgtggcgac agctcccatc cccggtgtga ggacgccgag cttgccctcg gccggggcga
     4081 tgttctctgc ttgtttcatg ttcctccgtc gagagacgat accgacggtc cgaccggagc
     4141 gatgaatcgt ccccggtcta gtgtcggtcc tgtgttggac tcgacacccg atggtctcgg
     4201 ccccaccgtc gacgtggcac tgctcgccga ctcggtccag gcagtccgcg gcaagctgtt
     4261 cgtgcttggt ggcgggtggg acacgctctg ggttccacgc ctccccgccc gtcaccccag
     4321 cctcgcaatc ggcctccgtc tccggatacc ggtctcgtgg aagtcggagt ccctgaagct
     4381 ctcagtggaa ctgcaggacg ccgacggcca ggccctcttg ccccgccccc tctcccatga
     4441 catcaagcta cccaccaacg ctcaaaaacc agctggggcg accgatttcg ggctggtcag
     4501 atcgttcacg ttcaacaacc tccggttcga cagcgaaggg gcatactcct tcgtgatctc
     4561 agtcgatgac cagccggtgt cacgggttcg cttcagtgtc cggatgaggc
//
LOCUS       DASUTA010000108         2541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1201924, whole genome shotgun sequence.
ACCESSION   DASUTA010000108 DASUTA010000000
VERSION     DASUTA010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201924"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..993
                     /locus_tag="VFZ80_02205"
     CDS             226..993
                     /locus_tag="VFZ80_02205"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX6286270.1"
                     /translation="MFRRWPVTTFYLLTLIVSWPAGLLPPGPSIAAIAATALIDGRSG
                     VVELVRRVVVWRVNVGWYLLALLGPLALFALAAGVNTLMGASPVGRLSLDWSEFGRLL
                     LLQIVGVFTGAWEELGWRGYALPRMMSRLSPLVASLGLGVLWAIWHVPLFLAGDIPWA
                     DAAFIVAVSVLFTAVFVQTSQSVLIAFLMHASINAAGGVTVLLFEGGDRTQMYWAVSA
                     VTAVVVVAVIAMRPRWWLNPPSGERIATAVGTFGEPD"
     gene            1108..1803
                     /locus_tag="VFZ80_02210"
     CDS             1108..1803
                     /locus_tag="VFZ80_02210"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX6286271.1"
                     /translation="MAIAGLVVFAVWLIPTSSATQNEVFDGEVDQLVVHVTGGVDLIA
                     GERTELAITKEWLFSGEPTVTVDHENGVARVEGECSWYQISCSTSVSGTVATDAAVEV
                     RASAGSISVGGTTRGVDLETSAGSVTVDDITGTARLVTSAGNITGTLADGDVDAETSA
                     GRIELMVLGEFASLSASTSAGNVDLTVPDDVYDVDADTSAGSVNINVRTDPAAGKRIV
                     AESSAGSITIDTP"
     gene            1807..>2541
                     /locus_tag="VFZ80_02215"
     CDS             1807..>2541
                     /locus_tag="VFZ80_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286272.1"
                     /translation="MTAPQLASGKSGWTFRVDSLVKVFDGGASPANDGLTLEVSPGEV
                     YGLLGPNGAGKSTLVKQVMGLLKPTSGSILLGDYDLVADPTVARQLCCYLPQAQMPID
                     SFKAREAVEIAGRIRGASPADIRRRTDELMSALEIEEWADTFGAKLSGGVRRLVGFAM
                     ATVCPGPIVFLDEPTNDVDPMRRRLLWERIRAVAGEGSAVFLVTHNVLEAEKSVDRLA
                     IINEGRLVAQGTPSSLKAEDRGCMRML"
BASE COUNT          436 a          777 c          859 g          469 t
ORIGIN      
        1 acaccaggtg cctacctcat gccgcctcgg gggacatgag atctggtggg aggtcgaagc
       61 agccgatgaa accgatgccc ttgggcggct gcccaggttc gtgggggatc ggaccatcgc
      121 aattcgagtc aaccggacgg aaatcccatg agctcagacc cggtccaact gccagtgacc
      181 gagcgtgtag gccccactcg acagctgtgg caaggctcga ggtcattgtt ccgacgatgg
      241 ccggtcacga cgttctactt gctgacgctg atcgtctcct ggccagcagg cttgctgccc
      301 cccggaccct cgatcgcggc tatagcggcg acggccctca tcgatggacg ctccggtgtt
      361 gtcgagctag ttcgaagagt ggttgtgtgg cgggtgaacg tcggttggta ccttctggcc
      421 ctgcttggac ctctggctct cttcgccctg gcggcggggg tgaacacgct catgggtgct
      481 tcgcctgttg gcagactgag cctcgactgg tctgagttcg ggagactcct tctgcttcag
      541 attgtcggcg tcttcaccgg cgcctgggag gagcttggtt ggcggggata cgcgctgcct
      601 cgaatgatga gccgcctctc gcccttggtg gcgagtctcg gtctcggcgt tctctgggcg
      661 atctggcacg tgccactgtt cctggcgggg gacatcccct gggcagacgc ggccttcatc
      721 gtggctgtct cggtcctctt caccgcagtg ttcgtgcaga ccagtcagag cgtcctcatc
      781 gccttcttga tgcatgcgtc catcaatgct gccggaggcg tcaccgtgct gctcttcgag
      841 ggcggtgacc ggacccagat gtactgggcc gtcagcgccg tgaccgcagt cgtggtggtg
      901 gcagtgatcg cgatgcggcc cagatggtgg ctgaatccgc catcgggaga acggatagcg
      961 acagccgtcg gcacctttgg tgaaccggac tgagacccag gaggaggaga gatgacatcg
     1021 gtcacaccaa cacctgaagt tcgatcatca ccgggggagc agccgcgccg aaagaggcgg
     1081 gtctggctat gggcccttga ggccctggtg gcgatcgctg ggttggtcgt cttcgctgtg
     1141 tggctgatcc cgacgagctc cgccacccag aacgaggtat tcgacggcga agtcgatcaa
     1201 ctggttgtcc atgtcaccgg tggcgtggac ctgatagccg gcgaacgcac cgaactggcg
     1261 atcaccaaag agtggctgtt ttccggggag cccaccgtga cggtcgatca cgagaacggt
     1321 gtggcccggg tcgaggggga atgctcctgg taccagatca gctgctcgac cagtgtgagt
     1381 gggacagtgg cgaccgacgc cgccgtcgag gtgcgcgctt ccgccgggtc gatctccgtc
     1441 ggtggcacga cccgtggcgt cgacctggaa acgtcggcgg gatcggtgac cgtcgatgac
     1501 atcacaggga cggcccgact cgtgacctcg gcaggcaaca tcaccggcac gttggccgat
     1561 ggcgacgtcg acgccgagac gtcggcaggt cgaatcgagc tgatggtcct cggtgaattc
     1621 gcctcgctgt ccgcgagtac gagcgccgga aacgtcgacc tcaccgtccc cgacgatgtc
     1681 tacgacgtcg acgccgacac ctcggccggc tcggtgaaca tcaacgtcag gaccgaccct
     1741 gcggccggga agcgcatagt cgctgagtcg agcgcaggct ccataacgat cgacaccccc
     1801 tgagccatga ctgcacccca gctggcgagc gggaagagtg gctggacttt cagagtcgac
     1861 agcctggtca aggtgttcga cgggggagcc tcgcccgcca acgacgggct cacactcgag
     1921 gtgagcccgg gggaggtcta tggcctcttg gggccgaatg gggcgggcaa gtcgacgctg
     1981 gtcaaacagg tcatgggtct gctcaagccg accagtggca gcatcctcct cggggactac
     2041 gacctcgtcg ccgaccccac cgttgcgcga cagctctgct gctacttgcc tcaagcccaa
     2101 atgccgatcg actccttcaa ggcccgggag gctgtggaga tcgccggtcg gatccgaggc
     2161 gcttccccgg cggatatcag gcggcgcacc gacgagctga tgtccgcgct cgagatcgag
     2221 gaatgggctg acacgttcgg tgccaagctg tcgggcggag tgcgacggct ggtcgggttt
     2281 gccatggcga ccgtttgtcc cgggccgatc gtcttcctcg acgagcccac caacgacgtc
     2341 gacccgatgc gacgccggct gctctgggag cgaattaggg cagtcgccgg cgaaggatcc
     2401 gcagtgttcc tggtgactca caacgtcctg gaagcagaga agtcggtgga ccgtctcgcc
     2461 atcatcaacg agggccggct cgtcgcccaa gggacgccct catcgctcaa ggcggaggac
     2521 agaggatgta tgaggatgct g
//
LOCUS       DASUTA010000109         2663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1202128, whole genome shotgun sequence.
ACCESSION   DASUTA010000109 DASUTA010000000
VERSION     DASUTA010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202128"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1041
                     /locus_tag="VFZ80_02220"
     CDS             115..1041
                     /locus_tag="VFZ80_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013733861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286273.1"
                     /translation="MTSPAVSTSGQAAALDHAAIVCEGLVKIYKVEDIEVVALQGLDL
                     VVDEGEFVAVVGASGSGKSTLLNILGGLDTPSAGKVAVAAFDLLNLDNRARTIYRRNI
                     VGFVWQQTARNLLPYLTAQENIEFPMTLNGAGAAHRRHRAMELLEIVGLADRARHKPS
                     ELSGGEQQRVAIATAIANSPRVVFADEPTGELDNTTSSEVFDALRNLNEDHGVTIVVV
                     THDPLVSEHVNRTVAIRDGRTSSEVLRRTEIGTGGEETTIAEEFVVLDRAGRLQLPHE
                     YVDALELERRVRLTLDPDHINVWPDREEAGDE"
     gene            1034..1735
                     /locus_tag="VFZ80_02225"
     CDS             1034..1735
                     /locus_tag="VFZ80_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006128165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286274.1"
                     /translation="MSDAVLGTAMLQAHGLTRIYSTGPSEVHAIEDVSIAVEPGSFVV
                     VRGRSGSGKTTLLNTLGGLDRPTAGTVSIAGQDLGDLDEAGLADLRRHEVGYIFQAFG
                     LIPILTAQENVEIPLRLVNAPPKQRRERARLLLDQVGLGGRSGHRPEELSGGEQQRVA
                     IARALANAPRLLLADEPTGQLDSRTGKTIIDLILELVRSQDIAAIVATHDPVPLAVAD
                     RVIELVDGRVVSDSG"
     gene            complement(1752..>2663)
                     /locus_tag="VFZ80_02230"
     CDS             complement(1752..>2663)
                     /locus_tag="VFZ80_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014986955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6286275.1"
                     /translation="EDSGLDVYGLGEHHRPDFASSAPAVVLAAAAGKTSKIRLFPAVS
                     ILSTDDPVRVFQQYATLDAVSDGRAELLVGRGSFIESFPLFGYSLDDYNDLFAEKLDL
                     LLAIRDTEGEIEWSGRFRSPIQGQGVYPRPDRPLPVRVAVGGTPQSIVRAATRGLPVA
                     LAIIGGNPDRFTAFAELHRKTLVENGFDPEEVPLSVHAHGYVADSVDQAVQEYYPSYA
                     AMMTRLGRERGWGPMTREQFDWLRSSEGSLVLGDPETVAAKILHFEEVLGVNRFELHV
                     SVGTLPHEQVLRSIEMLGTKVAPMVNG"
BASE COUNT          503 a          857 c          862 g          441 t
ORIGIN      
        1 cgtcgccgtg atggtcttgt cgttgatcgc agcccagtcg gtggccatcg gtatcttcat
       61 gctggtgagc cgacgccagg agctgagccg gttactcagg acgggtgatg agcaatgacc
      121 agccctgctg tctccacgtc cggacaagca gcagctctag accacgctgc catcgtctgc
      181 gagggtttgg tcaagatcta caaggtggag gacatcgagg tggtggccct gcaaggcctc
      241 gacctggtag tggacgaagg cgagttcgtt gccgtggtgg gtgcgtcggg tagcgggaag
      301 tccactctgt tgaacatcct tggagggctg gacacaccct ccgccggcaa agtcgcagtc
      361 gccgcattcg acctcctcaa cctcgacaac cgggcccgaa ccatctatcg acgcaacatc
      421 gtcggattcg tctggcaaca gaccgcacgc aacctcctcc cctatctcac tgcgcaggag
      481 aacatcgagt tcccgatgac cttgaatggc gccggcgcag cgcaccgacg tcatcgtgca
      541 atggagctcc tcgagatcgt cgggctcgcc gaccgggcac gtcacaagcc cagcgagctg
      601 tccggaggcg agcagcagcg cgtggcaatc gccactgcaa tcgccaattc gccgcgggtc
      661 gtgtttgccg acgagcccac gggtgaactc gacaacacca cgtcatcgga ggtgttcgac
      721 gccctgcgca acctcaatga ggaccacgga gtgacgatcg tcgtggtgac tcacgacccg
      781 ctcgtgtcag agcacgtcaa tcgcaccgtc gccatcaggg acggtcgcac cagcagcgag
      841 gtcctacgaa ggaccgagat aggtactgga ggcgaggaga ccaccatcgc cgaggagttc
      901 gtggtgctcg accgggccgg ccggctgcaa ctgccccatg agtacgtaga cgcgctcgag
      961 ctggagcgaa gggtccggct gaccctggac cccgaccaca tcaacgtgtg gccggatcga
     1021 gaggaggccg gcgatgagtg acgcagtgct gggcaccgcg atgttgcaag ctcacgggtt
     1081 gaccaggatc tactcgaccg ggccgagtga ggtccatgcc atcgaagacg tgagcatcgc
     1141 cgtggagccg gggtcgttcg tggtcgttcg tggtcggtcc gggagcggca agaccaccct
     1201 cctcaacaca ctcggcgggt tggaccgccc aacggccggc accgtgagca tcgccggcca
     1261 ggacctgggc gatctcgacg aagccggtct ggccgatctc cgtcgacacg aggtgggcta
     1321 catcttccag gcgttcgggc tgatcccgat cctcaccgcc caggagaatg tcgagatccc
     1381 gttgcggctg gtcaatgccc cacccaaaca acgcagggag cgtgcccgtc ttctcctgga
     1441 ccaggtcggt ttgggtggcc ggtcgggtca tcgccccgaa gagctctccg gaggcgagca
     1501 gcagcgggtg gcaatcgccc gcgctctggc caatgctccc cggcttctcc tcgccgacga
     1561 gcccacgggt cagctcgact cgcggaccgg gaagaccatc atcgacctca tcctcgaact
     1621 ggtcagatcg caagacatcg ccgccatcgt cgccacgcat gacccggtgc ccttggcagt
     1681 ggccgaccgc gtcatcgagc tggtcgatgg tcgggtagtg agcgatagcg gctgagcgtc
     1741 agggacgaca gctagccgtt gaccatgggg gccactttgg tgcccagcat ctcgatcgat
     1801 cggagcactt gctcatgggg caaggtcccc accgacacgt ggagctcgaa cctgttgaca
     1861 ccgagcacct cctcgaagtg gaggatcttg gcggcaacgg tctctgggtc gccgaggacg
     1921 agcgagccct cagacgagcg aagccagtcg aactgctccc gggtcatcgg cccccatccc
     1981 cgctcccttc ccaatcgggt catcattgcc gcataagacg ggtagtactc ctgtacggcc
     2041 tgatccacac tgtctgcgac gtagccatgg gcgtggaccg atagcggcac ttcctcgggg
     2101 tcgaagccat tctcgaccag ggtcttgcga tgcagctcgg cgaaggcggt gaaccggtcg
     2161 ggattgcccc cgatgatggc cagggcgacg ggaagcccgc gggtggcggc ccggacgatc
     2221 gactgcgggg ttccaccgac cgcgacacgc acggggagcg ggcgatcggg acgggggtag
     2281 accccctggc cctgaatcgg tgagcgaaac cgaccggacc actcgatctc accctctgtg
     2341 tccctgatcg ccagcaacag gtcgagcttc tcggcgaaga ggtcgttgta gtcgtcgagc
     2401 gagtagccga agagagggaa cgactcgatg aatgatcccc tgcccaccag cagttcggcc
     2461 ctcccatcgg agaccgcatc gagggtggcg tactgctgaa agacacgcac cgggtcgtcg
     2521 gtgctcagga tggagacggc cgggaaaagc ctgatcttcg acgtcttgcc ggctgccgcc
     2581 gcgagaacga cagcaggagc cgacgaggcg aagtccggtc tgtggtgctc ccccaacccg
     2641 tagacgtcga gacccgaatc ctc
//
LOCUS       DASUTA010000110         2550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_555060, whole genome shotgun sequence.
ACCESSION   DASUTA010000110 DASUTA010000000
VERSION     DASUTA010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555060"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..562)
                     /gene="ybeY"
                     /locus_tag="VFZ80_02235"
     CDS             complement(98..562)
                     /gene="ybeY"
                     /locus_tag="VFZ80_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX6286276.1"
                     /translation="MSVFVADEQRETVGLPDLHALAELVLREEGYPDDAEVTLLFVDE
                     TEIASYNGRFLDRDGPTDVLAFPVEEMLPGVIPDTDPQGPPLMLGDVVIAPSYVRRQA
                     GEHGATFEDEMALMVTHGILHLLGYDHVEDDEAERMEQRERDLLAKVGKRRR"
     gene            complement(627..>2550)
                     /locus_tag="VFZ80_02240"
     CDS             complement(627..>2550)
                     /locus_tag="VFZ80_02240"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX6286277.1"
                     /translation="SRAAIPDGFVPSGDNLGQLVDCVAGEDCSADPMGFMVTLTAADE
                     VLVEMRARHQPVPDQTIEYLIETASADVTRAALGEMSHLQNIRVVALDRLSSEFSRRI
                     TEDDLQEVKAGMVSSASPVLQDDGPDPEGGVAASEVVAAYLQANYLVNESSLAELREE
                     AAAEVPDVTVDYTEDQAIVQIGEPMTQLHIDAIEATSAPNLQEQPEAGLGAVLVVLVS
                     LIGVYLATFRPEFWSRPRMVSLLGIMIVLAAGSVRATVAAQRALTDAPGDPGWYVLPA
                     VAFGFVTAVLFDQRIAVLMALALGVLTAAGTLDTGATVYAILAALAPIPFVSAVSTRG
                     SFRNAVVLSSLVAAGVAAATAWFFHVGPNESVFEVIGVAVAWAFGVSVVASLLGLAAL
                     QFFESAFDITTTLGLLDLTDRNHKALQMLQDSAFGTFNHSLMVGTLADAAARSIGANA
                     LLARAMAYYHDLGKTENPTYFIENQFGIPNPHDLLDPRESAEIVRSHVTSGVALARQY
                     NIPTDVTEGIVSHHGDGVMRYFYEKARQEQGVDVDIDDFRHIGHKPRTAETAILMLAD
                     SLEAACRANFQSAEPTPDAIEKVVDRVMDEKLEDGQLSESPLTLGQISKIRRAFLESL
                     VGHYHQRIAYPNFPGS"
BASE COUNT          481 a          779 c          825 g          465 t
ORIGIN      
        1 gcatcagcgc ggggaactcg cgtgattgcc gtgccggcgc tcctcagcca cgcggccaac
       61 agcagcagga tgcctgcgac tatcaaggcg ccggctgtca tcgtctcctc ttacccacct
      121 tggcgagcag gtcacgctct ctttgttcca tgcgctccgc ctcatcgtcc tcgacgtggt
      181 catagcccag gaggtgcaag atcccgtggg tcaccatcaa tgccatctca tcctcgaagg
      241 tggcaccgtg ctctccggct tgacgccgca cgtacgaagg agcgatgacc acgtcgccca
      301 gcatcagcgg cggtccttgc ggatcggtgt ccgggatcac gccgggcagc atctcctcga
      361 cagggaaagc aagtacgtcg gtgggaccgt cacggtcgag aaagcgcccg ttgtaagagg
      421 cgatctcggt ctcgtcgacg aacaggaggg tcacctcggc atcatcggga tatccctcct
      481 cgcggagcac cagctcggcg agggcatgga gatcgggcag gcccacggtt tctctctgct
      541 cgtcggccac gaacacgctc atccgtgctc ggcctggtgt cgggtgcggg gcgggagccc
      601 ctgagtacta gaggatgggt cgtccatcag ctgccgggga agttcgggta cgcgattcgt
      661 tgatggtagt gaccgacgag tgactcgagg aaggctcgcc tgatcttgga gatctggcca
      721 agagtcaggg gcgactcgga gagctggccg tcctcgagct tttcgtccat gactctgtcc
      781 accaccttct cgatggcgtc aggggtcggt tcggcggatt ggaagttggc acgacaggct
      841 gcctccaaag agtcggccag catgaggatt gcggtctcag cggtgcgcgg cttgtgcccg
      901 atgtggcgga agtcgtcgat gtccacgtcg acaccttgct cctggcgggc tttctcgtag
      961 aaatagcgca tgactccgtc cccgtgatgg ctgacgatgc cctcagtcac gtcggtcggg
     1021 atgttgtatt ggcgagccag ggccacaccg gaggtgacgt gacttcgcac gatctccgct
     1081 gattcccggg ggtcgagaag gtcgtgggga ttggggatcc cgaactggtt ctcgatgaag
     1141 taggtcgggt tctctgtctt gcccaggtcg tggtagtagg ccatggcccg tgccagcaga
     1201 gcgttggcgc ctatcgaccg ggcggcggcg tcggccaggg tgccgaccat cagcgagtgg
     1261 ttgaaggtcc caaacgcgct gtcctgcagc atctggagcg ccttgtggtt gcgatccgtg
     1321 agatcgagca gtccaagggt ggtggtgatg tcgaaagccg attcgaagaa ctggagggcg
     1381 gcgaggccga gaagcgaggc aaccaccgac actccaaacg cccaggccac agcgacaccg
     1441 atgacttcaa acacagattc attcggtccg acgtggaaga accacgcggt ggcggcggcc
     1501 acaccggctg cgacgaggga cgaaaggacg acggcattcc tgaaggagcc acgagtgctc
     1561 accgcagaca cgaatggaat cggggccaag gcggccagga ttgcatacac cgtggcaccg
     1621 gtgtccagtg ttccggcggc ggtgagaacc cccaaggcca acgccatgag gacggcgatt
     1681 ctctggtcga atagcaccgc agtcacgaac ccaaaggcca ccgccggcag cacataccaa
     1741 cccgggtcgc cgggtgcgtc cgtcagagcc ctttgggcgg cgacggtggc gcggaccgac
     1801 cctgctgcga gaacgatcat tatcccgaga agggaaacca tccgaggtcg cgaccagaac
     1861 tccggccgga atgtggccaa atacacaccg atcaaagaga cgagcacgac gagcaccgcc
     1921 ccgagcccgg cctccggctg ctcctggaga ttgggagcgg acgttgcctc gatggcgtcg
     1981 atgtgaagct gggtcatggg ctcgccgatc tggacgattg cctggtcctc ggtgtaatcg
     2041 acggtcacgt cgggaacctc ggcggcagct tcctcacgca gctcggcaag actcgactcg
     2101 ttgaccaggt agttagcctg cagataggca gctacgacct cactcgcagc aacgcctcct
     2161 tcgggatcgg gcccgtcgtc ctgcaggacc ggtgatgcac tgctgaccat ccccgccttg
     2221 acctcttgca ggtcgtcctc tgtgatccgc cgactgaact cagaggagag gcgatcgagc
     2281 gccaccaccc ttatgttctg gagatgggac atctctccga gagctgctcg ggtgacgtca
     2341 gccgatgcgg tctcgatcag gtactcgatc gtctggtctg gcacaggctg gtgtctggct
     2401 ctcatttcca cgaggacctc atcagccgcg gtgagagtca ccatgaagcc cattggatct
     2461 gcggaacagt cctcgccggc aacgcagtcc accagttgac cgagattgtc gccactggga
     2521 acgaacccgt cgggtattgc ggccctactc
//
LOCUS       DASUTA010000111         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1719862, whole genome shotgun sequence.
ACCESSION   DASUTA010000111 DASUTA010000000
VERSION     DASUTA010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719862"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VFZ80_02245"
     CDS             <1..618
                     /locus_tag="VFZ80_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017544158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6286278.1"
                     /translation="GFEVEVADSGVKGLDLARRGNPDLVLLDLMLPEMSGLDVCRLIR
                     SSSDVPIIMLTAKDSEADKVTGLELGADDYMTKPFSMRELVARVRAHLRRAAKTGVLA
                     ESNEVLRGGPIELDIDAHVTRLNGDEVELRPKEFELLESLMRRKNRLAARHTLIDEVW
                     GPSYFGDTKTLDVHIKRLREKLETDSSNPRHIVTVRGLGYKFVDE"
     gene            611..>2062
                     /locus_tag="VFZ80_02250"
     CDS             611..>2062
                     /locus_tag="VFZ80_02250"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX6286279.1"
                     /translation="MSSRPYRTKLSSRFVAYYAITYLVMIGLMGFIVDRSARAALIDE
                     VDETLAVVAGLAQESLPSDESRYARWAQETFQASGFRVTLIDVDGVVRADSHSDPEIM
                     ENHIDRPEIQIALTGEVGESSRVSESTGFEQRYLALPPVDGLMVRTSVATRIIDDELL
                     QVRRSIVVAGVVLGALGVGLMVFLARRLTRPIVELTEQAGAVAEGEIEVQPRRSRVEE
                     LDQLGAAISTMANRLGSRLTEAERAMSTLEVVLGALPQGTILIDGDDRIVYVNTAAGD
                     ILGAVPEALVNLAPLQFQNAVRDARLKKQTQSRVLDHGSPTRRLRGVATPFSDDDRVL
                     LLVVDITERERTDTIRRDFVANASHELKTPVSTIIASSEALQIALDRGDASAAAFADR
                     IESSARQLDRLVGDLLDLSRLEKESPEVNPARLDHLVREEVERMRRDAVAKGIDIVIQ
                     TDEVIATVNQRDVAIAVRNLLDNAIRYTPEDGSI"
BASE COUNT          385 a          651 c          637 g          389 t
ORIGIN      
        1 ggcttcgaag tcgaggtggc cgattctggt gtcaaaggcc tcgatctggc acgcaggggg
       61 aatcccgatc tggttctgct cgacctgatg ctccccgaga tgtcgggtct cgacgtgtgc
      121 cgtctcatcc ggtcgtcctc ggatgtaccc atcatcatgc tcaccgccaa ggactccgag
      181 gcggacaagg tcaccggtct cgagctgggc gcagatgact acatgaccaa gcccttctcg
      241 atgagagagc tggtggcgag ggtgcgagcc catctcagac gcgccgccaa gacgggggtg
      301 ctcgccgagt ccaacgaggt gctcaggggt gggcccatag agctcgacat cgatgcccat
      361 gtcacgcgcc tgaacggcga tgaggtcgag ttgaggccca aggagttcga gcttctcgaa
      421 tccctcatgc ggcggaagaa ccgcttggcc gctcgtcaca cactcatcga cgaagtctgg
      481 gggccttcgt acttcggtga caccaagacc ctcgacgtgc acatcaagag actccgggag
      541 aagctggaga ccgactcctc gaacccaagg cacatcgtca ccgtgagagg attgggctac
      601 aagttcgtcg atgagtagtc ggccttaccg aaccaagctg tcctcgaggt tcgtcgccta
      661 ttacgccatc acctacctgg tgatgatcgg gctcatgggc ttcatcgtcg accggtcggc
      721 tcgtgccgct ctcatcgacg aggtcgacga gacccttgcc gtcgtcgccg gtctcgccca
      781 ggaatcgctg ccttcggacg agtcccgcta tgcgaggtgg gcgcaggaga cctttcaggc
      841 gagtgggttt cgggtgacac tcatagacgt cgatggtgtg gttcgtgccg actctcactc
      901 tgacccggaa atcatggaga accacatcga ccgacccgaa atccagatag ccctgaccgg
      961 agaagtcggg gagtcgagcc gagtcagtga gagcaccggc ttcgagcagc gctatctggc
     1021 gctccctcct gtcgacggac tgatggtgcg cacctcggtg gccacgcgaa tcatcgacga
     1081 cgagctgttg caggtgcgcc gttccattgt cgtcgcgggt gtcgtcttgg gtgcactcgg
     1141 tgtcgggctc atggtgttcc tggctcgacg gctgacccgg cccatcgtcg agctcacgga
     1201 gcaggccggt gccgtggccg agggagagat cgaggttcag cctcgtcgct cccgggtgga
     1261 ggagctcgat cagttgggtg ctgcgatatc cacgatggcc aaccgtctcg gatcccgcct
     1321 gaccgaggcg gagcgcgcca tgtcgacact cgaggtcgtc ctcggcgcac ttccccaagg
     1381 caccatcctc atcgacggtg acgatcggat cgtctacgtg aacacagccg ccggtgacat
     1441 tctcggagct gtcccggagg cgttggtcaa tctcgccccg cttcagtttc agaacgcggt
     1501 gcgcgatgcc cgtctgaaga agcagaccca gtctcgtgtc ctcgatcacg ggagccccac
     1561 gcgacgcctt cgtggtgtgg cgacgccatt ctccgacgac gacagagtgc ttctgctcgt
     1621 agtcgacatc accgagcgag agagaacgga cacgataagg cgcgacttcg tcgccaacgc
     1681 atcacacgag ctcaagaccc cggtctcgac gatcatcgcc tcttcggagg ccttgcagat
     1741 cgccctggat cgcggcgatg cttcggcggc cgctttcgcc gatcgaatcg agtcttccgc
     1801 ccggcagctg gatcgtctcg tcggtgacct gctcgatctg tcccgcctcg agaaggagag
     1861 ccctgaggtc aatccggccc gtctcgacca cctcgtccga gaggaggtgg agcgaatgag
     1921 acgagatgca gtggccaagg gcatcgacat agtcatccaa accgacgagg tcatcgccac
     1981 cgtcaaccag cgtgatgtgg cgatcgccgt tcgcaacctc ctcgacaacg caatccgcta
     2041 cacccctgaa gacggctcga tc
//
LOCUS       DASUTA010000112         5570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1461586, whole genome shotgun sequence.
ACCESSION   DASUTA010000112 DASUTA010000000
VERSION     DASUTA010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461586"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..724
                     /locus_tag="VFZ80_02255"
     CDS             71..724
                     /locus_tag="VFZ80_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX6286280.1"
                     /translation="MFTDRTQAGRRLAERLISYKAKRPVVLGLPRGGVPVAAEVAKAL
                     NAPLDIIVVRKLGLPGHSEYAMGAIGEGDVRYVDWQVVSEFGVSASQLSRVVEREQAE
                     MGRRVDRYRAHRPRVAVDGRTAIVVDDGIATGSTAVAAVRVARALGAESVVVATPVAP
                     QDTVERLQRVADQVVVLETPSPFYAVGQAYLDFDQVGDEEVAQILGRSLVEHFDPAA"
     gene            complement(796..1215)
                     /locus_tag="VFZ80_02260"
     CDS             complement(796..1215)
                     /locus_tag="VFZ80_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX6286281.1"
                     /translation="MDLKVWTPFPYLDREWRFDFPRFFRETDEFRPSMDVVRTNGTLV
                     LTAELPGMTADDVDISLEGDVLTIKGEKTDEREVSDADRYVHERTFGSFHRRMTVPDG
                     VTAKAIDAKFENGILTVQVTLPEVKEVEPKKIPVTAN"
     gene            complement(1346..2788)
                     /locus_tag="VFZ80_02265"
     CDS             complement(1346..2788)
                     /locus_tag="VFZ80_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6286282.1"
                     /translation="MTLEGMVQHGLLDAMADGLLVCDEHGAIRAVNRRIETLSGYGAE
                     ELIGRQVEILVPRSLRDQHERHRAGFGGSGHPARPMGADLDTRVLTKDGSTLPVDIAL
                     APLEIGSEILVVTAVRDATVRVRQERSIRAMREVTESILKGRPPGDIYALICERAVDL
                     LRASVALIAVRAERGDQFKILAAAGGIGESLAGRLLAHPFWRAALGTDGWTTFDADSE
                     HLSEALGHPIGPAVILPITEGQRLGFLLVADELGCPPFAADSLDRLETFASQAGLAFA
                     YARRLRALAIAGDRDRIARDLHDLVIQRLFGAGLSLQVVSQLFGGAEPALSQRLDEVI
                     ESIDAAIAELRRSIFDLERKDELGLRDRVLIAVEEVLAEHEINVTVRMDEVVVEPAEE
                     NASHLLATLREALTNVGRHSAATAVDISVSNGQDLVLIVKDNGVGMGLDTSMGHGLSN
                     MAWRARQLGGALDVSNGPRGGLRLQWRVPG"
     gene            2878..3279
                     /locus_tag="VFZ80_02270"
     CDS             2878..3279
                     /locus_tag="VFZ80_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286283.1"
                     /translation="MRSVMYVYYFVHLGGEPQMWAYRLQADGAWLRRMAEEALLGVDG
                     RPREGDLLEIGDSRPIGRGFSIPIAWTAPGLFVGLVGDVSLQPLETGITQMAIRGSYR
                     SDHDGVDGTGDNHRKVETVVKRLLDSVWLGS"
     gene            3335..4096
                     /locus_tag="VFZ80_02275"
     CDS             3335..4096
                     /locus_tag="VFZ80_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010985800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6286284.1"
                     /translation="MIAGGARFDMTEQRRRWHGQIETTGESGGLMSSHTANRPAQIVL
                     VDDHHVVRSGLKSLIDFESDLVVVGEAGSVEDAVELVSKDPPDLVVMDVRLPDGSGIS
                     ACHHITTLFPDVKVMILTSYANDVALSSAVAANAAGYVLKNVRAAELISDIRHVLEGV
                     KIFDRVGEDRFEEPLLTALSPQELTVADHLADGLTNREIAREMDLAEKTVKNYVSNVL
                     TKLGMTRRSEAAAHVARIRAMTGASIAGRRGMNAG"
     gene            4116..5270
                     /locus_tag="VFZ80_02280"
     CDS             4116..5270
                     /locus_tag="VFZ80_02280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6286285.1"
                     /translation="MKLIDLTEAEGRMGPRVGMVARFPPSTSASAPGSSELAEKLLHR
                     YGIPVEVIRLVLPGERGATTLPVVMDLNPRWHMSARLAGQRANRCDIAIVQIERHIPV
                     AMIDELVSELTIPVILCIDDVPPADSEEAAAMAAISRRVDHVVVHSEVARGRLEAAAG
                     DPIPIEVIPHGSPWESMEPPIGGRGRILTWGFMAPGMGAERVVRALPRLADLEPLPEY
                     RVVGLPDPRWPRREVDAYRDGLLAEAEALGVADRFELVPIYHTRKELAAEIAASDLIA
                     VVYDSKDRSASRILTEALSAGRPVIATSFPGAIEMLATGAGTTVAHDDEEGLAREIRR
                     YLSDEGEYRNASSRAAAISAQVNWGEIARRFAGLLTARSQDRQGSIESLS"
     gene            5313..>5570
                     /locus_tag="VFZ80_02285"
     CDS             5313..>5570
                     /locus_tag="VFZ80_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase-like protein"
                     /protein_id="HEX6286286.1"
                     /translation="MVTVKRSDLEIRPDPKRVVARPFLPGEAAFSGGPTRVELIAGRV
                     LGLDEAQADKILAETRAEFASRHSDLDAVWEQNFQWAAGMVP"
BASE COUNT         1047 a         1707 c         1770 g         1046 t
ORIGIN      
        1 tcttttgtga ctctcgactc tgttcagagt cgcttctcga tgtgacgatg gcagtgaata
       61 ggcaggtgtc atgttcactg acagaaccca ggcgggacgg cgtctcgccg agagactcat
      121 ctcctacaaa gccaagaggc cggttgtact cgggctcccg cgtggaggag tcccggttgc
      181 tgccgaagtc gccaaggcct tgaatgctcc cctcgacata atcgtcgtcc gcaaactcgg
      241 actacccgga cactccgaat acgccatggg cgccattggg gaaggcgatg ttcgctacgt
      301 ggactggcag gtggtctccg agttcggcgt cagtgcctcc cagctctccc gcgtagtgga
      361 acgcgagcag gccgagatgg gtcgcagggt tgatagatac agagcacacc gaccccgcgt
      421 cgcggtggac gggcggacag cgatcgtggt cgacgacggc atcgccaccg gatcgaccgc
      481 ggtggcggcg gtgcgggttg cacgagccct gggagcagaa tcggtcgtgg tggccactcc
      541 tgtggcgccg caagacaccg ttgagcggct acagcgggtt gccgaccagg tcgttgtcct
      601 ggagacgccc tcgccgttct acgcggtagg acaggcctat ctcgacttcg atcaggtcgg
      661 tgacgaagag gtggctcaga tactggggcg atcactggtc gagcacttcg atcccgccgc
      721 ttgacgacgg tttccaacac tggaagggcg gacccggatc gggtccgccc ttccgcgtcc
      781 ccccagacgg acggctcagt tagccgttac ggggatcttc ttcggctcga cttccttcac
      841 ctcgggaagg gtgacctgaa cggtcagaat cccattctcg aatttggcat cgatcgcctt
      901 ggcggtcacg ccatccggca ccgtcatacg gcggtggaac gagccgaatg tccgctcgtg
      961 gacataacga tcggcatcgg agacctctcg ctcgtctgtc ttctcccctt tgatggtgag
     1021 gacatcacct tcgagagaga tgtcgacatc atcggcggtc atacccggga gctcggcggt
     1081 aagcaccaga gtcccgtttg tccgcacgac atccatggaa ggacggaatt cgtccgtctc
     1141 gcggaagaag cgggggaagt cgaagcgcca ctccctgtcc agatagggga acggcgtcca
     1201 taccttgagg tccattgact cacctccttc tgggccacga ttcacacccg gtgggggccc
     1261 ggttcgaccg ggatctcaat cgcgatcatc tgctgtatcg cgacggttgg ccagagtcat
     1321 ttggcccttt tgggttgaga tgcactcagc ctggcacgcg ccactgcagc cggaggccgc
     1381 cgcgggggcc gttggacacg tccagtgctc cgccgagctg gcgggcccgc caggccatgt
     1441 tcgaaagacc atgacccatc gaggtgtcta gccccatccc aactccgttg tctttgacga
     1501 tgaggaccag gtcctggcca ttcgagaccg agatgtccac cgccgtcgct gcggagtgac
     1561 gtccgacatt ggtgagggcc tcgcgcaagg tggcgaggag gtgcgaggcg ttctcctctg
     1621 ccggctcaac aaccacctcg tccattctca cggtcacgtt gatctcgtgt tcggcaagca
     1681 cctcttccac tgctatcagc acccgatcgc gaaggccgag ctcgtccttg cgctccagat
     1741 cgaagatgct gcgccgcagt tcggcgatgg ccgcatcgat gctctcgatc acctcgtcga
     1801 ggcgttgtga caacgccggt tcagcgccgc cgaacagttg ggagacaacc tgtagcgaca
     1861 gtccggctcc gaacaaccgc tgtatcacca gatcatgcag gtcgcgcgct atgcggtccc
     1921 tgtcaccggc aatcgccagc gctcggaggc gccttgcgta ggcgaaagcc agccctgcct
     1981 gagatgcgaa ggtctccaaa cgatcgaggc tgtcggcagc gaaaggcggg cagccgagct
     2041 catcggcgac gagcagaaag ccaagtctct ggccctccgt tatcggcaat atcacagctg
     2101 gcccgatcgg gtgacccaat gcttcgctca ggtgctcgga gtctgcatca aaggtcgtcc
     2161 agccatcggt gcccagtgct gccctccaga aaggatgcgc cagaagacgt cccgccagac
     2221 tctcaccgat gccgccggcg gccgccagga tcttgaattg atcgccccgc tcggcccgta
     2281 ctgcgatgag agcgaccgaa gcacggagca agtcgactgc tcgctcgcag atcagtgcat
     2341 agatgtcacc cggggggcga cccttcagaa tcgactcggt gacctctctc atcgcccgga
     2401 tggagcgttc ttgacggacc cggacagttg cgtctctcac cgcggtgacg accaggatct
     2461 cggagccgat ctcgagggga gccagggcga tgtcgacggg cagagtgctg ccgtccttgg
     2521 tcaggacacg ggtgtccaga tcggccccca ttggacgggc gggatggcca gatcccccaa
     2581 atcctgcccg atggcgctca tgctggtcgc gtaacgaacg aggtaccaga atctctacct
     2641 gccgcccgat aagctcttct gcgccgtatc cggagagggt ctcgattcgg cggttgaccg
     2701 ctctgatcgc cccgtgctcg tcgcagacca agagaccgtc agccattgcg tccaacaacc
     2761 cgtgttggac catcccctcg agggtcatca gcgagtcact tctccgccgc ggactatata
     2821 gcgagcgctt ccactcgggt ccttcggccc tggtgacctg tgcggcatca gggtcaaatg
     2881 agatcggtga tgtacgtgta ttacttcgtg catctcggtg gggagccgca gatgtgggcg
     2941 tatcgtctcc aggcagacgg ggcctggctt cgtcgaatgg cggaggaggc cctgctcggt
     3001 gtcgatggcc ggcctcgtga aggcgacctc ttggagatcg gtgatagtcg tccgatcggg
     3061 agaggcttct ccatccccat cgcctggact gcacccggcc tgttcgtggg tctcgtgggc
     3121 gatgtcagtt tgcagccatt ggagaccggc atcactcaga tggcgattcg agggtcatac
     3181 cggtcggacc atgatggcgt tgacggcaca ggcgacaacc atcggaaggt ggaaaccgtc
     3241 gtgaaacgtc tgctggactc ggtctggctc gggtcttaga agtggcctgc ccggccaaaa
     3301 gcccggccct ggcagtgacc ttcgccccta tccgatgatc gcgggcggtg ccagattcga
     3361 catgaccgag cagaggagac gttggcacgg tcagatcgag accacaggcg aatccggagg
     3421 gttgatgtca agtcacactg cgaaccgacc ggctcagatc gtcctggtgg acgatcacca
     3481 tgtggtgcgg tccggcctca agagcctcat cgacttcgag tccgacctcg tcgtcgtggg
     3541 tgaggccggg tcggtggaag atgcggtgga actggtctcg aaggatccac ccgatctggt
     3601 cgtgatggat gttcgccttc ccgacggctc cggcatatca gcgtgtcatc acatcacgac
     3661 tctgtttccc gacgtcaagg tgatgatcct cacttcctac gcaaacgacg tcgctctctc
     3721 ctcggcggta gctgcaaacg cggcaggcta tgtgctgaag aacgtgaggg cagcggaatt
     3781 gatctcggat atcagacatg tcctagaggg tgtgaagatc ttcgaccggg taggagaaga
     3841 tcgcttcgag gagcccctcc tcacggcatt gtcaccgcag gaactgaccg tcgcagacca
     3901 tctcgccgat ggcctgacca accgtgagat cgcccgggag atggacctgg ccgagaagac
     3961 ggtcaagaac tacgtctcca atgtcctcac caagctggga atgacccgac gcagcgaggc
     4021 agccgctcat gtggcccgca ttagagcgat gaccggagcg tcgatcgcgg gtcgccgagg
     4081 aatgaatgct ggctgaagac gggtaagggc ggcctatgaa gttgatcgat ctcaccgagg
     4141 ccgaagggag aatgggccca cgagtgggca tggtggctcg tttccctcca agcacgtccg
     4201 catcggctcc gggctcgtcg gagctcgccg agaaactcct tcaccgatac gggatccccg
     4261 tcgaggtgat ccgtctcgtc ctccccggtg aacgaggtgc caccacactt cccgtggtga
     4321 tggacctgaa tccgcgatgg cacatgagtg cccgactcgc ggggcagaga gccaatcgct
     4381 gcgatatagc gattgttcag atcgagcgtc acatccccgt cgcgatgatc gacgagcttg
     4441 tctctgagtt gactatcccc gtcatcctgt gcatcgacga cgttccgccg gcggacagcg
     4501 aggaggcagc ggcgatggcg gcgatatcga gacgagtgga tcacgttgtc gtccactcag
     4561 aagtggctcg cggacgtctc gaggcggcgg caggcgaccc gattccgatc gaagtcatac
     4621 ctcacgggtc gccatgggag tcgatggaac cgccaattgg agggcgagga agaatattga
     4681 cctggggatt catggccccg ggcatgggag ccgaacgcgt agtccgtgct cttccccggc
     4741 tagctgacct ggagccgctc ccggagtatc gggtcgtcgg cctgcccgat ccgagatggc
     4801 cacgacgaga agtcgacgca taccgtgacg ggctcctcgc cgaagccgaa gcactcggcg
     4861 tcgccgatcg gttcgaactg gtgccgatct accacacgag gaaggagctt gcagccgaga
     4921 tcgctgcaag tgacctgatc gcagtggtct atgactcgaa ggaccgttcc gcctcacgca
     4981 tcctcaccga ggctctctcg gcggggcgac ctgtcatcgc cacctcgttt ccgggtgcca
     5041 tcgagatgct ggccacgggt gccggtacca cggtggccca cgacgacgag gaggggcttg
     5101 cccgggagat tcgtcggtac ctgagcgatg agggcgaata caggaacgct tcgagcagag
     5161 cggcagcgat cagcgcccag gtcaattggg gcgagatcgc ccgcagattc gccggtcttc
     5221 tcaccgcccg atcgcaagac cgccaaggtt cgatcgagag cctgtcatga gattccggct
     5281 acttgccggt gaacggacca tcatcgttgg gaatggtgac cgtcaagcgc agtgatctcg
     5341 agatcagacc cgaccctaag cgtgtcgtcg ccaggccttt cctgccgggt gaggcggcct
     5401 tctcgggagg gccgacccgg gtcgagctga tcgccggcag ggtgctcggc ctcgatgagg
     5461 cccaagccga caagatcctt gctgagacca gggcggagtt cgcttcgagg cactccgatc
     5521 tcgacgcggt atgggagcag aacttccaat gggcagcggg gatggtccca
//
LOCUS       DASUTA010000113         4036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_424871, whole genome shotgun sequence.
ACCESSION   DASUTA010000113 DASUTA010000000
VERSION     DASUTA010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424871"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="VFZ80_02290"
     CDS             <1..994
                     /locus_tag="VFZ80_02290"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6286287.1"
                     /translation="DPSTFPGVMHIVAFGTLLATTAMILLFGGVLESANNTAWSILAV
                     IGAVALFADRRAHFWLAAFVVTTTGAFLVTRFVEPTYVLPSRGYFALFNTLAVAVFVY
                     GILYYFVRQSARLYDQSETLLRNILPAPIAERLKHSTDMIADEFDSASILFADIAGFT
                     PMSAAMEPNEVIELLNEVFTAFDSLVADRRLEKIKTIGDAYMVAAGVPVPRDDHARVI
                     CDLALAMQEYLRGTDFAGHDLQMRIGIASGPVMAGIIGHQKFSYDLWGDTVNLSSRME
                     TSGIPGRIQLTESTCRLVQDWFECEERGQVEIKGKGPTTTWFLVGPRSATDRPG"
     gene            1074..1811
                     /locus_tag="VFZ80_02295"
     CDS             1074..1811
                     /locus_tag="VFZ80_02295"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEX6286288.1"
                     /translation="MITSGLGRRHALSWVALAAVFTLPLAGCVDGIIGVRGSGNMTTE
                     NRDVVGFSEIVLESSGEVNIEMGDAESLTIEAEDNLMSHLTSDVENGQLVLGTSGSIV
                     TTRNIVYTITATELSGITVAGSGDVEVADVTTTDFSTVISGSGTVDVTGLSADSLEVA
                     ISGSGQIDMTGTADRIDVEIPGSGSFDGEEMESATADVSISGSGDAVVNVSETLNANV
                     SGSGSIEYLGSPEVSSNISGSGSITPR"
     gene            complement(1872..2570)
                     /locus_tag="VFZ80_02300"
     CDS             complement(1872..2570)
                     /locus_tag="VFZ80_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286289.1"
                     /translation="MSRRLLACSLVAILAVGACGGGEGGDEATDTTEAGGTPTTEAPV
                     TSTEGTDADDDGGQTVDIGDIPQECVEAFVGYLRAIEPVVEEVDWANASLADFEDIGA
                     SLESITEEYEAEIADSNCEDLDVDASGEESFQYMIDLASREAPGTVPYFEMIRDFAAG
                     VGEGSDIVVSNDCETDIATMQAIVDEGVPMQDMPANELGAMGGLITSISANCSQERSF
                     EFFSQEDVAAYMAG"
     gene            2723..3013
                     /locus_tag="VFZ80_02305"
     CDS             2723..3013
                     /locus_tag="VFZ80_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286290.1"
                     /translation="MGGNAEMVSFNFDPTEGPHIASGLSDQELDFLQQVLAIELAIRM
                     DDASYQMRFHKIAEHRVEMDEIVSDWRRQRDRMKFVESVTSDLDQLMVLEER"
     gene            complement(3016..3918)
                     /locus_tag="VFZ80_02310"
     CDS             complement(3016..3918)
                     /locus_tag="VFZ80_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013286050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX6286291.1"
                     /translation="MTATIKVGTCSWTDPTLTETEDFYPDTNMTAEERLGFYAQHFPI
                     VEVDSTYYAPPSERVAGLWVERTPEHFTFDVKAFRLLTQHPTPPKSLWKGIREKLPAE
                     QSDKKNVYMRDLPRDLQSEAAQAFRDALMPLHSAGKLGVVLFQLPPYVYPTRGSFGYL
                     KWVAAELDDFQLAVEFRNGRWLDDENWTDTLDFLERHSLAYVCVDEPQGFKSSVPPVV
                     AVTAPVAEVRFHGRNAENWEKKGITAAERFRYDYPREELEEWVPRIETLAEGADSVHA
                     LMNNCYSDYGIRSAKSLGDLLDIF"
BASE COUNT          758 a         1249 c         1251 g          778 t
ORIGIN      
        1 ggatccatcc acgttcccgg gcgtgatgca catcgttgcg ttcggcacgc ttctcgcaac
       61 gacggcgatg atcctcctct tcggtggtgt tctcgagtcg gccaacaaca cggcgtggag
      121 cattctcgct gtcatcggcg ctgtggccct cttcgccgat cgacgcgctc acttctggct
      181 agccgcgttc gtcgtcacca ccaccggagc cttcctcgtg accagattcg tggagccgac
      241 ctatgtcctc ccgagtcggg gctacttcgc tctcttcaac acgctggcgg tggctgtgtt
      301 cgtatacggg atcctctact acttcgtgag gcagtcggcg cggctgtacg accagtccga
      361 gacgctgttg cgcaacatcc tcccagcgcc gatcgccgag cgtctcaagc actccactga
      421 catgatcgcc gacgagttcg attctgcctc cattctcttc gctgacatcg ctgggttcac
      481 tccgatgtcg gcagccatgg agcccaatga ggtcatcgag ctgctgaacg aagtgttcac
      541 cgccttcgac agcctggtcg ctgatcgcag gctcgagaag atcaagacga tcggagacgc
      601 ctacatggtc gccgccggag tcccagtgcc ccgcgacgac cacgccagag tgatctgcga
      661 cctcgctctg gcaatgcagg agtacctcag ggggaccgac ttcgccggtc acgacctcca
      721 gatgcgaata gggatcgcct cgggcccggt gatggccggg atcatcggcc accagaagtt
      781 cagctacgac ctttggggag acacggtcaa tctctccagc cgaatggaga cgtccggcat
      841 cccgggtcgg atccagctga ccgagagcac atgtcgcctg gttcaggatt ggttcgaatg
      901 tgaggagcgg ggacaggtcg agatcaaggg caagggcccc acaacgactt ggtttctcgt
      961 cggtcccagg tcggcgacag atcgacccgg ttgaaagagc cgtttgaccc tgccgtccgg
     1021 aaacagggag agtcatgctc aactcgatga gtgatcttcg agaaaggacg gaaatgatca
     1081 cgtcaggttt gggtcgccga cacgctttga gctgggttgc tctggcagct gtcttcacgc
     1141 tgccgctggc cggatgtgtc gacggaatca tcggtgtccg gggatcgggc aacatgacca
     1201 cagagaatcg ggatgtcgtc ggcttcagtg agatcgtcct cgagagcagc ggggaggtga
     1261 atatcgagat gggtgatgcc gagtcgttga ccatcgaggc cgaagacaac ctgatgtccc
     1321 atctcacctc cgatgtcgag aacggccagc tggtcctggg cacgtccggc tccatcgtga
     1381 ccacacgcaa catcgtctac acgatcaccg ccacggagct ctcgggcatc accgttgccg
     1441 gcagtggcga cgtcgaggtg gccgacgtca caacgacgga cttctcgacc gtgatcagcg
     1501 gctcgggcac ggtcgatgtg accgggctgt ccgctgacag cctggaggtg gccatctccg
     1561 ggtcgggcca gatcgacatg accggcactg ccgaccggat cgacgtcgaa atacccggtt
     1621 ccggcagctt cgacggtgaa gagatggagt cggccaccgc cgacgtgtcg atctcggggt
     1681 cgggagacgc cgtcgtgaac gtctctgaga cgctgaacgc caacgtttcg ggctcgggca
     1741 gcatcgagta tctcgggtct cctgaagtga gctcgaacat ctctggatcg ggaagtatca
     1801 cgccgcgttg acccagcaca tcggtcggcc gagggcccgt ggaggacgag aggctcaacg
     1861 cgccgcttgg ctcaacctgc catataggcg gcgacgtcct cctgagagaa gaactcgaag
     1921 gaccgctcct gactgcagtt ggcgctgatc gacgtgatca atccacccat ggcccccagt
     1981 tcgttggcgg gcatgtcctg catggggact ccctcatcga cgatggcctg catcgtggcg
     2041 atgtcggtct cgcagtcgtt gctcacaacg atgtccgagc cctctcccac acccgctgca
     2101 aagtcgcgga tcatctcgaa gtagggcacc gtccccgggg cctcccgact cgccagatcg
     2161 atcatgtact ggaacgactc ttccccgctg gcatcgacgt ccaggtcctc gcagttggaa
     2221 tcggcgatct cagcctcgta ttcctctgtg atcgattcca gtgacgctcc gatgtcctcg
     2281 aagtcagcta acgaggcgtt cgcccagtcg acttcctcga caacgggctc gattgcgcgg
     2341 aggtacccga cgaaggcttc gacgcactcc tgggggatgt ccccgatgtc aacggtctgc
     2401 ccaccatcgt cgtcggcgtc ggtgccctcg gtggaggtca ccggtgcttc ggtcgtcggc
     2461 gtaccccctg cttcggtggt gtccgttgcc tcgtcaccgc cctctccacc accacaagct
     2521 ccgacggcga gaatcgccac cagcgaacag gccagaagtc tccgtgacac cccaacaacc
     2581 cctttcggac tgcgcgcaac gtgcgcgaac gcgcgcagca taggacttct ccgggctctc
     2641 tgatgctcgg gaacacaccg tttctcggag ggagatcccg caccaggacc acgtatctcc
     2701 gggatggaag ggccctcctt tcatgggagg gaacgcagag atggtgagtt tcaacttcga
     2761 cccaacggaa ggcccgcaca tagcgtcggg attgagcgat caagagctcg acttcctcca
     2821 gcaggtgctg gccatcgagc tggccatccg aatggacgac gcctcatacc agatgcgttt
     2881 ccacaagatc gccgagcacc gagtggagat ggacgagatc gtctccgact ggcgtcgcca
     2941 gcgtgacagg atgaagttcg tcgagtcggt cacctcggat ctcgatcaac tgatggtgct
     3001 cgaagagcgc taggactaga agatgtcgag gaggtcaccc aggctcttgg cggagcggat
     3061 gccgtagtcg gagtagcagt tgttcatgag ggcgtgcact gagtcggcgc cctcggccaa
     3121 ggtctcgatg cgaggcaccc actcctccag ctcctcgcgc ggatagtcgt agcggaagcg
     3181 ctcggcagcg gttatgccct tcttctccca attctcggca ttgcgaccat ggaaccggac
     3241 ctcggcgacc ggggcggtga ccgccaccac aggggggacc gagctcttga acccttgcgg
     3301 ctcgtcgacg cacacatatg caagggagtg gcgctccagg aagtccaagg tgtcagtcca
     3361 gttctcgtcg tctagccagc ggccgttgcg gaattccacg gcgagctgga agtcatccaa
     3421 ctcggcggct acccatttca gatatccgaa cgatcctctg gtcggataca cgtacggcgg
     3481 caactggaag agcaccacac ccagcttccc ggcggaatgg aggggcatca gggcatctcg
     3541 gaaagcctgg gctgcctccg actgcaggtc ccggggcagg tcgcgcatgt agacgttctt
     3601 cttgtccgac tgttcggcgg ggagcttctc tctgattccc ttccagagtg atttcggagg
     3661 agtggggtgc tgggtgagca gcctgaaagc cttgacgtcg aatgtgaaat gctcgggcgt
     3721 gcgctcgacc cacagtcccg caacccgctc cgaaggaggg gcgtagtagg tggagtccac
     3781 ctcgacgatg gggaagtgct gagcgtagaa accgagccgc tcctcggcag tcatgttcgt
     3841 gtcggggtag aagtcctcgg tctcggtgag tgtggggtcg gtccaagagc aggtcccgac
     3901 cttgatcgtc gctgtcatcg accccgagcc tacgagcgat tgcggacagt tccggtcatg
     3961 ggcgtgtgca gatacggcca ggccacggta tctgcacacg ctcgaaagcc gagggtctga
     4021 tcggcgactg aggccg
//
LOCUS       DASUTA010000114         2523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1332484, whole genome shotgun sequence.
ACCESSION   DASUTA010000114 DASUTA010000000
VERSION     DASUTA010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332484"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /gene="uvrB"
                     /locus_tag="VFZ80_02315"
     CDS             <1..1638
                     /gene="uvrB"
                     /locus_tag="VFZ80_02315"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011605724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX6286292.1"
                     /translation="SATAGLLVRDDVIIVASVSAIYGLGSPEQYMGQLLRLVQGVEYP
                     MKDAIQRLVDIQYERNEVNLIRGKFRVRGDTLEVFPSYEETIVRVQFWGDEVERIIRL
                     NPVTGEVIDELPELFVFPATHYVTERERMLKAIAGIEEELVERLAELEGQGKMLEAQR
                     LRMRTNYDLEMMREVGFCSGIENYSRHLDGRAPGEAPYTLLDYFPKNYLTIIDESHVT
                     IPQIGGQYEGDHSRKEVLVEHGFRLPSAKDNRPLRFDEWLEKANQVVFLSATPSEFEI
                     SRSTQVVEQIIRPTGLIDPEVMIRPTKGQIDDLLDEIRERALREQRVLVTTLTKKMAE
                     DLTDYLLEMGVRARYMHSEIDTLERVQIMRELRLGDFDVLVGINLLREGLDLPEVSLV
                     AILDADKEGFLRSETSLIQIIGRAARNVDGQVVMYADNVTESMKRAINETNRRRRAQT
                     KWNAEHGIDPQTVRKRVSDILELVQSEAPRGDRRRREVEPRRPIDLEGDDLTRLILSL
                     EDEMHEAAKELRFEYAARLRDEVNELKRELREVAEAG"
     gene            1648..>2523
                     /locus_tag="VFZ80_02320"
     CDS             1648..>2523
                     /locus_tag="VFZ80_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286293.1"
                     /translation="MIRPVRPAAGEDSAVDTGDLQRRTIGPVFIALLILSPAASSAVS
                     IWASNVAQIPKIQRLVVVAGAFVLVGIVVFWALWRLLVDTRVAAFITFFIVLALTSGG
                     RILESRPWALRWLAAASVVAVVALIVHRLRDSWLLDAIMAACAAALLLPGLMSGVWAS
                     VARVDPTPSAGVPDPAAVMGQRPDVVLVILDGYTSLPVLREVFGYEDPSLRPDLAALD
                     FEVVEPIFSPYSMTHLSVGSLLELDYLATDAQSLEVEDGRTLAQVIGGDSALVDLLSG
                     NGYQTTMVEPGWHMSV"
BASE COUNT          466 a          801 c          808 g          448 t
ORIGIN      
        1 tcggcgaccg ccggtctcct ggtccgtgac gacgtcatca tcgtcgcgtc ggtctccgcc
       61 atctacggcc tgggctcacc cgagcagtac atggggcagc tgctccgact ggtccaaggg
      121 gtggagtacc cgatgaagga cgccatccag cgtctcgtcg acatccagta cgagcgcaac
      181 gaggtcaacc tgatccgggg gaagttccgg gtgaggggcg acacactcga ggttttcccg
      241 tcgtatgagg agaccatcgt gagggtccag ttctggggcg acgaggtgga gcggatcatc
      301 cggctcaacc cggtgaccgg cgaggtgatc gacgagctcc ccgagctgtt cgtcttcccc
      361 gccacccact acgtgaccga gcgggagcgc atgctcaagg cgatcgccgg catcgaggag
      421 gaattggtcg agcgtctcgc cgagctggag ggccagggca agatgctcga ggcgcagcgg
      481 ctgcggatgc ggaccaacta tgacctggag atgatgcgcg aggtcggctt ctgctccggg
      541 atcgagaact actcgcgcca cctcgacgga cgggctccgg gagaggctcc ctacaccctg
      601 ctcgactatt tccccaagaa ctatctcacc atcatcgacg agtcccacgt gaccattcct
      661 cagatcggcg ggcagtacga gggtgaccac tcccgcaagg aggttctggt cgagcacgga
      721 ttccgtcttc cctcggccaa ggacaaccgg ccgctccggt tcgacgaatg gctggagaag
      781 gccaaccagg tggttttcct ctccgccacc ccgtccgagt tcgagatctc gcgatcgacc
      841 caggtggtgg aacagatcat ccggcccacc ggactgatcg accccgaggt gatgatccgc
      901 cccaccaagg gtcagatcga cgaccttctc gacgagataa gggaacgcgc cctgcgcgag
      961 cagagggtcc tggtaaccac cctgaccaag aagatggccg aggacctcac cgactatctg
     1021 ctcgagatgg gagtcagggc aagatacatg cactcggaga tcgacaccct ggaacgggtc
     1081 cagatcatgc gagagctccg tctcggtgac ttcgacgtct tggtcgggat caacctgctg
     1141 cgagagggcc tcgacctgcc cgaggtgtca ctggtcgcga tcctcgacgc ggacaaggag
     1201 ggctttctcc gctcggagac ctcactgatc cagatcatcg gccgggcggc gagaaacgtc
     1261 gacgggcaag tggtcatgta tgccgacaac gtcaccgagt cgatgaagcg ggcgatcaac
     1321 gagaccaacc ggagacgccg agcccagacc aagtggaacg ccgagcacgg gatcgacccg
     1381 cagaccgtgc gaaagcgcgt ctccgacata ctggaactgg ttcagtcgga ggctccccgc
     1441 ggcgatagaa gacgacgtga ggtcgagccg agacggccca tcgacctcga gggtgacgac
     1501 ttgaccaggc tgatcctgag cctggaggac gagatgcatg aggcagccaa agagctcaga
     1561 ttcgaatacg cagcccggct tcgcgacgag gtcaacgagc tcaaacggga gctacgcgag
     1621 gtggccgagg ccggctgacc tcacgtcatg attcgtccag tccggcccgc ggcaggggag
     1681 gactcggccg tcgacaccgg ggacctccag aggagaacga tcggcccggt cttcatcgcg
     1741 ctgctcatcc tctccccggc cgcctcgtcg gcggtgtcga tatgggcgag caatgttgcc
     1801 cagatcccga agatccaacg gctggtcgtg gtcgccggcg cattcgtcct cgtcgggatc
     1861 gtcgtcttct gggcgctttg gcgtctcctc gtcgatacac gcgtggccgc gttcatcacc
     1921 ttcttcatcg tccttgcgct cacatctggc ggcaggatcc tggagtcccg gccgtgggct
     1981 ctccgctggt tggcggcggc cagcgttgtg gccgtggtcg cgttgatcgt gcaccgtctc
     2041 cgcgattcgt ggttactcga cgcgatcatg gccgcgtgcg ccgcggccct cctcctcccc
     2101 ggcctgatga gtggtgtctg ggcctccgtt gcccgcgtcg acccaacccc atcggcgggg
     2161 gtgcctgatc ccgcagcagt gatggggcaa cgtcccgacg tagttctggt gattctcgac
     2221 gggtacacca gtctgcccgt gctccgtgaa gtgttcggat atgaagatcc gagccttcgg
     2281 cccgaccttg ccgctctcga cttcgaagtg gtagagccga tcttctcgcc ctactcgatg
     2341 actcacctat cggtgggcag cctgctggag ctggactacc tggccactga cgcacagtcg
     2401 cttgaagtcg aggacggcag gacattggcg caggtgatcg ggggcgacag cgccctggtc
     2461 gatctccttt ccggcaacgg ataccaaacc accatggtgg aaccgggctg gcacatgtcg
     2521 gta
//
LOCUS       DASUTA010000115         2912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_425276, whole genome shotgun sequence.
ACCESSION   DASUTA010000115 DASUTA010000000
VERSION     DASUTA010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425276"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VFZ80_02325"
     CDS             complement(<1..617)
                     /locus_tag="VFZ80_02325"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX6286294.1"
                     /translation="MIIDLHTHSTASDGSDPPSRVVELAAQAGLGAIALTDHDTQEGV
                     GEALTAARSVGLEVIPGVELSLQFDGGGMHLIVLWLEPGGGPLQDRLEGLQLGRGARN
                     ERIVELLAAAGMPITVEEILEEAGGGTVGRPHIAAVMMKRGYVPDIRTAFDLWLGAGK
                     PAYAGRDRLYPEEALSLARESGAVPVLAHPHTLGITTASAMADVL"
     gene            complement(649..1437)
                     /locus_tag="VFZ80_02330"
     CDS             complement(649..1437)
                     /locus_tag="VFZ80_02330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX6286295.1"
                     /translation="MSDRRQRQKEQRAAKREAERKHESRRALFRSIGTALLFGLIVVA
                     IFTFGGIFDDGTSDIPASYEDYREQETACGATQPEPEQAMSFEGPTDQNIPDDAVVTA
                     TLATSCGEIAMELDNTGAPETVNSFVFLAREGFYDGQVIHRISENFVFQTGDPSADGT
                     GGPGYVVADEFPDDEDFVYEPGVVAMANRGSRSTGSQFFVVVGDDGQFLTNNFNVLGR
                     VSSGQETIDRIMEVPTATAPGSNEQSRPLETVYIESITIDVADS"
     gene            1523..2503
                     /locus_tag="VFZ80_02335"
     CDS             1523..2503
                     /locus_tag="VFZ80_02335"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX6286296.1"
                     /translation="MPVVVQKYGGTSVADPDRIKAVADRVVQTRESGHDLVVVVSAMG
                     QTTNTLLGLAAQVSKSPQPREMDMLLTAGERISMALLAMALNERNVPAVSYTGSQAGI
                     LTDSAHGGARITKITGERVRASIDEGKVVIVAGFQGVDPTSKEVTTLGRGGSDTTAVA
                     LAATLRAESCEILTDVAGVFTADPRVVQEARLLADVGYEQMMALANAGAGVLMPHSVE
                     TARDSEVPVHVRSSFTHDEGTWVRSGVPDRPFVGIAHRLDDGHGVITVVGSEAHSTAT
                     DEMVAMQEAGIVTALISSDEQSVSLDVSPEQTEAAVRLLHARLFQMEATS"
     gene            2500..>2912
                     /locus_tag="VFZ80_02340"
     CDS             2500..>2912
                     /locus_tag="VFZ80_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX6286297.1"
                     /translation="MTTVAVVGATGAVGRTMLAILAEREFPADTLRLAASARSAGQTV
                     DTCLGSVVVEDLDTMDPTGIDIALFSAGGARSKEHAPRFADAGAVVVDNSSAFRMDPA
                     VPLVVVGVNDGKASGHEGIIANPNCTTMTLMMAAGP"
BASE COUNT          517 a          935 c          926 g          534 t
ORIGIN      
        1 tcgagcacat cggccatcgc cgatgcggtg gtgatgccca gggtgtgagg atgggccagg
       61 acaggcactg cgccagattc tcgcgccagc gagagagctt cctcgggata gagccgatcc
      121 cgtcctgcat aggcgggctt ccctgctccc agccagagat cgaacgccgt gcgtatgtcg
      181 gggacgtacc cccgcttcat catcaccgcg gcgatgtgag gtcgacccac ggtcccgccc
      241 ccggcctctt cgaggatctc ctcgacggtt atcggcatcc cggcggccgc cagcagctcg
      301 actatccgct cattgcgggc tcctctcccc agctggaggc cttccagcct gtcttggagc
      361 gggccacctc ccggttcgag ccagaggacg atcagatgca tgccgccacc gtcgaattgg
      421 agcgacagct cgactccggg gatgacctcg aggccgacgc tcctggctgc ggtcagcgcc
      481 tcgccgacac cctcctgtgt gtcatggtcg gtgagagcga tggctcccag gccggcttgt
      541 gcggccaact cgacgacccg ggaaggagga tctgatccgt cggacgcagt cgagtgagtg
      601 tggaggtcga tgatcatcgg ttggcaccga tgatgcctgg tacgaccgtc aagaatcggc
      661 tacgtcgatc gttatcgatt cgatgtatac cgtttcgagc ggccgactct gctcgttgga
      721 acccggtgcc gtcgctgttg gcacctccat gatccggtcg atcgtctcct gaccggagct
      781 cacccgaccc aggacgttga agttgttggt caggaactga ccgtcgtccc cgacgaccac
      841 gaagaactgc gacccggtgc tccgtgatcc gcgattggcc atggcgacca caccgggctc
      901 atagacgaag tcctcgtcat cggggaactc gtcggccacc acatagcctg ggccgccggt
      961 cccgtcggca gacgggtcac cggtttgaaa gacgaagttc tcgcttatcc gatggatcac
     1021 ctgaccgtcg tagaagccct cccgagccaa gaagacgaag gagttgaccg tttccggtgc
     1081 cccggtgttg tcgagctcca ttgcgatctc accgcaggag gtggccaagg tggccgtgac
     1141 cacggcatcg tcggggatgt tctggtccgt cggcccctcg aatgacatgg cctgctccgg
     1201 ttcaggctga gtggccccac acgccgtctc ttgctcccgg tagtcctcat aagaggccgg
     1261 gatgtcggat gtcccgtcgt cgaagatccc gccgaaggtg aagatggcaa ccacgatcaa
     1321 cccgaaaagc agggcggtgc caatcgaacg gaacaaggcc cggcgggact cgtgcttgcg
     1381 ctcggcctcg cgtttggccg cgcgttgctc tttttgacgc tgacgtcggt cggacatggg
     1441 tgggcgcgac tctaggacac gggcgcggtc aattcgccat tgggttttcg taatcggaga
     1501 gccgacgttc caaactgtgg gtatgccagt cgtggtgcag aagtatgggg gcacatcggt
     1561 tgccgatccc gatcgaatca aagccgtggc cgacagggtg gttcaaactc gtgagagcgg
     1621 ccacgacctg gtggtcgtgg tgtccgcgat gggccagacc accaacacgt tgctcggtct
     1681 ggcagcacag gtgagcaagt ccccgcagcc gagggagatg gacatgctcc tcaccgccgg
     1741 agagcgaatc tccatggctc tacttgcgat ggctctcaat gagaggaacg tgcctgcggt
     1801 cagttacacc ggctcacaag caggcatcct caccgactcg gcccatggag gggcccgcat
     1861 caccaagatc accggtgaga gggtgcgggc atccatcgac gaggggaagg tcgtcatcgt
     1921 cgccgggttc cagggcgtcg accccacatc caaggaggta acgactctgg gacgaggagg
     1981 ctcggacacc actgccgtcg ccctcgccgc aacgctcaga gccgagtcgt gcgagatcct
     2041 gaccgatgtc gccggagtgt tcaccgccga tccgagggtt gttcaggagg cccggctcct
     2101 cgccgatgtc ggttacgagc aaatgatggc gctggccaac gccggagccg gagtcctcat
     2161 gccacattcg gtcgagacgg cgcgggacag cgaagtccct gtccatgtca ggtcctcatt
     2221 cacccacgat gagggcacct gggtcaggtc cggtgttccc gatcggccat tcgtcggcat
     2281 cgcccatcgt ctggacgatg ggcacggggt aatcaccgtg gtcggctccg aggctcattc
     2341 gactgccacc gacgagatgg ttgccatgca ggaagcgggc atcgtcaccg ccttgatcag
     2401 ctccgacgag cagtccgttt ctcttgacgt gagcccggaa caaaccgagg ccgccgtgcg
     2461 gttgctacat gctcggctct ttcaaatgga ggcgacttca tgacaactgt tgccgtggtt
     2521 ggagccaccg gcgccgtcgg ccggacgatg ctcgcgattc tcgccgagcg cgagttccct
     2581 gccgacaccc tccgtctcgc cgcctccgca cggtcggcag gccagaccgt cgacacctgt
     2641 ctgggatcgg tcgtcgtcga ggacctcgac accatggacc ccaccgggat cgacatcgct
     2701 ctcttctcag ccggaggggc caggtcaaag gagcatgcgc cgcggttcgc cgacgccggc
     2761 gcggtggtcg tcgacaactc ctcggccttc cgcatggacc cggcggtccc cctcgtggtc
     2821 gtcggagtga acgacgggaa ggcatccggt catgaaggga tcatcgccaa ccccaactgc
     2881 accaccatga ctctgatgat ggcggcaggt cc
//
LOCUS       DASUTA010000116         2212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_37928, whole genome shotgun sequence.
ACCESSION   DASUTA010000116 DASUTA010000000
VERSION     DASUTA010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37928"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VFZ80_02345"
     CDS             <1..451
                     /locus_tag="VFZ80_02345"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HEX6286298.1"
                     /translation="PFQVSNRFRSANFERHRRVGRVVLIAGLIAGGMAVVVGAVMPYG
                     GFAETSASTVFGIYFVTALALAYRAIRSGRVSSHRRWMIRAFALGVGVGLIRVVIGVG
                     EAVGIGISESFGAAFWIAFVTMTVLAEIWLKLRPDEVPHLIEIESVP"
     gene            complement(461..1183)
                     /locus_tag="VFZ80_02350"
     CDS             complement(461..1183)
                     /locus_tag="VFZ80_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6286299.1"
                     /translation="MTLAIHLYRRVMRRGRLIGLIALSSVPALAFWLSAYDSGPDEAA
                     VLYSDIVARLGFSYAIAVLILTVAILREERDAGTLPYIYMRPIGREAMAASSIAAGIA
                     AALTLALAGWLVSLLAVLALASPASTSLAGLAQYTAAGVGYAAVFVPLGYLVPRSILV
                     GLGYIIVVESILAEVVTGLAQVSIWRIALSISADIGANDDPGEMLDAIAPGVGGGIAK
                     MGVVLVLGFGVLVWALRRRDAL"
     gene            complement(1180..2100)
                     /locus_tag="VFZ80_02355"
     CDS             complement(1180..2100)
                     /locus_tag="VFZ80_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286300.1"
                     /translation="MTDTIVVEELSKWFGPKVAVSEVTIGFAPGVTGLLGPNGAGKTT
                     LMRVIAGLQRPSQGSVSILGEDPRAHPGIYQKIALVPEDEAVYERLSARRFVELAARL
                     AKVDDPKGRAQTALESVDLVGPSDRLLGGFSKGMRQRAKVAAALVSDPEVLLLDEPLN
                     GADPLQRAHLISLFKELGATGRTVLVSSHVLVEVERMTERVVAMVDGRLAAVGSVADI
                     RSAMTDRPRLVFIESSDNRSLAAALMSTVGVIGVHIEPGRLRVEASDAGDLAKRLPAI
                     AVAHGIGVNRVEPADESLESVFRYLVEGGE"
BASE COUNT          412 a          705 c          720 g          375 t
ORIGIN      
        1 ccctttccag gtgtcgaaca ggttccggtc ggccaacttc gagagacacc gacgggtggg
       61 cagggtcgtg ttgatcgccg ggctgatcgc cgggggtatg gctgtggtgg ttggggcagt
      121 gatgccgtac ggagggttcg ccgagacctc ggcgtcgacc gtcttcggca tctatttcgt
      181 caccgctctg gcactggcct accgcgccat ccgctcgggg agggtgtcct cgcatcgccg
      241 ctggatgatc cgcgcctttg ccctcggtgt gggtgtcggg ctgattcgcg tcgtgatcgg
      301 ggttggtgag gccgtgggca tcggcatcag cgagtccttc ggggcggcgt tttggatcgc
      361 tttcgtgacc atgacagtcc tggccgagat ctggttgaag ctgaggcctg atgaagtccc
      421 tcatctcatc gagatcgaat cggtcccctg aaactcctgc ctacaaggcg tcacggcgac
      481 gcagcgccca gacgaggacc ccgaaaccga gaaccagcac cacgcccatc ttggcgatgc
      541 cgccgccgac cccaggagca atcgcatcga gcatctcgcc ggggtcgtcg ttcgccccga
      601 tgtcggccga tatggagagg gcgatgcgcc agatcgagac ctgggcgaga ccggtcacca
      661 cctcggcgag aatcgactcc acgacaatga tgtagcccag tcccaccagt atcgatcgcg
      721 gcaccagata gcccagaggg acgaaaaccg cggcgtaacc gactccggct gcggtgtact
      781 gagccagccc tgcgagcgag gtgctggctg gggaggccaa tgccagcacg gcgagcagtg
      841 agaccaacca tccggccaac gccaacgtca gggccgcggc aatgccggcg gcgatcgacg
      901 aagccgccat cgcttcccgt ccgatggggc gcatgtagat gtagggcaga gtgccggcgt
      961 cgcgctcctc cctgaggatg gcgactgtga ggatgagcac cgctatcgca taagagaacc
     1021 cgagtcgggc aacgatgtcc gaatagagga cagcggcttc gtctgggccc gagtcgtagg
     1081 cagagagcca gaacgcgagg gcggggaccg atgacagcgc gatcaatccg atgagccgcc
     1141 cccgtctcat gacccgcctg tagagatgga tggcgagtgt cattccccac cttccaccag
     1201 atagcgaaag acagattcga gcgactcgtc cgccggctcg actctgttca caccgatccc
     1261 gtgggcgacc gcaatcgcgg gcagccgctt ggcgagatct cctgcgtccg acgcttccac
     1321 acgcagacgt cctggctcga tgtgaacacc gatcacaccg acggtcgaca tcagcgccgc
     1381 ggcgagactc cggttgtcgc tggactcgat gaagaccagc cggggccggt cggtcatggc
     1441 gctgcggatg tcggcgacac tgccgacggc ggcgagccgc ccgtcgacca tcgctacgac
     1501 tcgctccgtc atcctctcca cctcgacgag gacgtgggag gagacgagaa ccgttctgcc
     1561 ggtcgccccc aactctttga agagtgatat cagatgggcc cgttgcaagg ggtcggctcc
     1621 attgagcggc tcatcgagca agaggacctc gggatctgag acgagggcgg cagccacctt
     1681 ggctcgctgt cgcattccct tggagaaccc cccgagcagc cggtctgacg ggccgacgag
     1741 gtccaccgat tcgagtgctg tctgtgccct gcctttggga tcatcgacct tggcgagacg
     1801 agcggcgagc tccacgaaac gtcgtgcgct gagacgctca tagacggctt cgtcctcggg
     1861 aaccagagcg atcttctggt agatgcccgg gtgggctcgc gggtcctcac caaggatcga
     1921 aaccgatcct tgcgacggcc tttgtagacc ggcgatgacc cgcatgaggg tcgtcttgcc
     1981 cgctccgttc ggtccgagca gcccggtgac cccaggagcg aagccgatgg tcacctcgct
     2041 gacggcgacc ttcgggccga accacttgga gagctcctcg acgacgatgg tgtcggtcac
     2101 gccagcttcc gatacctgct gcgcacgaag atcacggcag caacaacgac gacggcgatg
     2161 aagatcaccg aagcccagac cgtgaaaccg gccatttcgg cggggtactc ga
//
LOCUS       DASUTA010000117         2013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_38063, whole genome shotgun sequence.
ACCESSION   DASUTA010000117 DASUTA010000000
VERSION     DASUTA010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38063"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VFZ80_02360"
     CDS             <1..1010
                     /locus_tag="VFZ80_02360"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HEX6286301.1"
                     /translation="VLVTGAGGSIGSEIGRQLLDMGAVVIALDHDETHLHDAMLAWPD
                     SHRATPVLCDIRDESDLEMVFSEALPDVVFHTAALKHVPMLESYPVQAVKTNVFGTVN
                     LLKASEMFDVSRFVLISTDKAVNPEGVLGSTKRVAEMLVQSAASTNGSRRFAVVRFGN
                     VLGSRGSVVPTFMGQIRRGGPVTITDPEMTRYFMTLQEAVGLVLQAGALAAQGEVFVL
                     DMGEPARIEDLAHRMIRLAGLVPGRDIDVVTTGPRPGEKTSEVLSNEPLRPSAHPKIR
                     LAGPGYPGPAKMLEILESFDQDIRTGDIESLRRNLKEVCWQDWQPNETVDLAALEGFV
                     S"
     gene            1045..>2013
                     /locus_tag="VFZ80_02365"
     CDS             1045..>2013
                     /locus_tag="VFZ80_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286302.1"
                     /translation="MHRLLLIVRSRWWAFLLVGLIGMAAGYLFATIRNDNIEPRYESE
                     ASVVVLRQSDDESGRELQGRLSEAQRAAIRVNTGVLAPGVAEIRTEIGETEGRLLFVG
                     YGETDVDAQALAASMRDDYLETAPVGVEAVEDQLSRYAEDVAAIRAQIAELGAEQQTE
                     EAVDPAVSLLSAELAELQQTAVQLAVDLALYDFRDLERERSDIESELETVRSAIFETQ
                     AQLDALQPDPELNTDRDLRVQALQQQEAELTQEYLDLFLNQAEASEITSLGVVETSEV
                     SATPVSPFLGAVGGFLLGLLVSLAVALLYDLVRKPVWSPADIATVPV"
BASE COUNT          354 a          617 c          661 g          381 t
ORIGIN      
        1 gagtgttggt gaccggggcg ggcgggtcga tcgggtccga gatcggccgc cagctgctag
       61 acatgggggc agtggtcatc gccctcgatc acgacgagac ccatctccac gacgcgatgc
      121 tggcctggcc cgactcccat cgcgccaccc cggttctctg cgacatcaga gacgagtccg
      181 atctggagat ggtcttcagc gaagcgcttc ccgatgtcgt gttccacacc gcagcgttga
      241 aacacgtccc aatgctggag agctacccgg tccaagcggt caagacgaat gtctttggga
      301 ccgtgaacct cctcaaagcg tcggagatgt tcgacgtatc acgtttcgtg ctcatctcga
      361 ccgacaaggc ggtgaatcct gagggtgttc tcgggtccac caagcgtgtg gccgagatgc
      421 tcgtccagtc ggccgcctcg accaacggct ccagacgttt cgccgtggtc cgcttcggaa
      481 acgtcttggg cagtcgcggt agcgtggttc ccacgttcat gggccagatt cgcaggggcg
      541 ggcccgtgac catcaccgac cccgagatga ccaggtactt catgactctc caggaggcgg
      601 tggggttggt gctccaggct ggtgccctgg cggcccaagg agaggtcttc gtgttggaca
      661 tgggcgagcc cgcccgaatt gaggacctgg cgcacaggat gatccgtctg gccggtctcg
      721 ttcccggtcg cgacatcgac gtggtgacca ccgggccgag acccggagag aagaccagcg
      781 aggtcctgtc gaacgagccc ttgcgaccga gcgcccaccc caagattcgg cttgccgggc
      841 ccgggtaccc agggccagcg aagatgctcg agattctcga gtcgttcgac caggacattc
      901 gcaccggcga tatcgagtcg ctgcgtcgaa acctcaagga ggtttgctgg caggattggc
      961 agccgaacga gacggtggac ctggcggctc tcgaggggtt tgtctcgtga gggccgctct
     1021 tcgttccggg aacggtgctt gaccgtgcac cggcttctcc tcatcgttcg gtcacgctgg
     1081 tgggcatttc tgctcgtcgg cctcattggc atggcggcgg gctatctctt cgccacgatc
     1141 cgcaacgaca acatcgagcc acggtacgag agcgaagcct cggtcgtcgt cctccgacaa
     1201 tccgatgatg agtccggtcg ggagcttcag ggaaggctga gcgaggctca acgcgcggcc
     1261 atcagggtga acaccggcgt gctcgctccg ggtgtcgcgg agatacggac cgagatcggt
     1321 gagacagagg gtcggcttct gttcgtggga tacggagaga ccgacgtcga cgcccaggct
     1381 ctggcggctt cgatgcgcga cgactacctg gagacggccc cggtcggtgt ggaagcggtt
     1441 gaagatcagc tctcgcgcta tgccgaagat gtagcggcga taagggcgca gatcgccgag
     1501 ctcggagcgg aacagcagac cgaagaggcc gtggatcccg ccgtctcact gttgtctgcc
     1561 gaactggccg aactccaaca gactgcggtg cagctggcag tcgacctggc cctctacgac
     1621 ttccgtgacc tggagcgaga gcgatcggat atcgagtcgg agttggaaac ggtgcgctcg
     1681 gcgatcttcg agactcaagc ccagctcgac gcgctccagc ccgatcccga gctcaacacg
     1741 gaccgtgatc tgcgtgttca ggccctccag cagcaggagg cagaactgac ccaggagtat
     1801 ctcgatttgt tcctcaatca ggcggaggcg tctgagatca cctcgctggg cgttgtcgag
     1861 acgagtgagg tcagtgccac accggtgtct cccttccttg gcgccgtcgg cgggttcctc
     1921 ctcggtctct tggtctccct ggcggtcgct ctcctgtacg atctggttcg caaaccagtg
     1981 tggtcgccag ccgacatcgc aacggtgccc gtg
//
LOCUS       DASUTA010000118         6088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1333487, whole genome shotgun sequence.
ACCESSION   DASUTA010000118 DASUTA010000000
VERSION     DASUTA010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333487"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..715)
                     /locus_tag="VFZ80_02370"
     CDS             complement(26..715)
                     /locus_tag="VFZ80_02370"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX6286303.1"
                     /translation="MKRIGILGGMSWQSTISYYRYINEEANSRLGGKHTADVLMWSGD
                     FDPIAACTDARDWGELARILVPAAKSLAAQGAEVMVIAANAAHSVADAVEAYGEVPVV
                     NIIDVTAHRMKESGVETAALLGTSAVMEEPFYRDRMAQNGIDCLVPSDETRAEINRIV
                     FEELVFGVVDDGSHRTLVDAIDVLADEGAEGTILGCTELSLILDEDDGVIPGFDTTRI
                     HAIAAVDAAMG"
     gene            complement(712..2187)
                     /locus_tag="VFZ80_02375"
     CDS             complement(712..2187)
                     /locus_tag="VFZ80_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HEX6286304.1"
                     /translation="MAKDKSKKKKPKAKKGSELKERRTSKQFEGKFGKTKIAYTATAG
                     TQVFESNGEKVSFFYVSYTQDDADPADRPLLFCFNGGPGSSTVWLNLGLYGPRRMEFD
                     EDGFKMGMQGRLVDNAYSILDVADVICIDAMGTGFSRTEKKGDEKKFHHFQRDIEAFS
                     EFIVHYLNRNGRWASPKYLSGESYGTLRGAGIARELFVAHNVEFNGIIFISMILNYQT
                     SAIDSENWLIHPGNDLPFALYLPTYAATARYHGQLAEVYQQMPLRDFLEEVEDFALGE
                     YWSALAKGDQIDDELRKEIAGRLAAYTGLDADFVDHYDLRVQIHRFCKELLREQHRTV
                     GRIDSRYTGIDRVKAGNVIEADPSIDATIGVAASALNHYLRDEIGFETDEIYKVMGLE
                     VWKNWDYETFKGKYVDTSENFRELLSRSRGTKVLVANGYYDLATPHFATEYTFSHMGL
                     DPEVRENVKLTYYEAGHMMYVHQASLKQLAADLREFVKATS"
     gene            complement(2283..2498)
                     /locus_tag="VFZ80_02380"
     CDS             complement(2283..2498)
                     /locus_tag="VFZ80_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286305.1"
                     /translation="MALCEYTEKLTLTPSAVGRDEISALREVGLDDEGISSATQVIAY
                     FNYINRIAEGLGVPLEEWLNHDGTPKE"
     gene            complement(2601..2846)
                     /locus_tag="VFZ80_02385"
     CDS             complement(2601..2846)
                     /locus_tag="VFZ80_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6286306.1"
                     /translation="MSHVHILSYEEATGIARREYDAAMRRAGRIWNIVSIQSQLPEVM
                     RDSMRIYLSIMYGPSPLTRAQREMMAVVTSQVNDCHY"
     gene            complement(2848..3801)
                     /locus_tag="VFZ80_02390"
     CDS             complement(2848..3801)
                     /locus_tag="VFZ80_02390"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX6286307.1"
                     /translation="MSEPDPILLFTGSGSRRLSAKIADHLGIELGASETRHFSDGNLF
                     VKVQQNVRGHDVFLVQSTVFPSNDNFMELLFWIDAFKRASARNVTVVIPYFSYGKGDK
                     KDEPRVSIRARVCADAIETAGADRVVAMDLHAPQIQGFFRVPVDNLYAISVFCDRIAA
                     EGRDDLVVVSPDAGFAREARRYATALGSGVAIADKQRKDHEELAEVWDIVGDVSGRHT
                     VIVDDFTISAGTLVSCANELMRLGAKSVFAAVTHGVFTQGSMERLDESPIEKLIVTDT
                     VENQPVEISEKVEVVSVAPLFAEAIERIHNRESISVMFGEY"
     gene            complement(3807..5192)
                     /locus_tag="VFZ80_02395"
     CDS             complement(3807..5192)
                     /locus_tag="VFZ80_02395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX6286308.1"
                     /translation="MPREFRLPDIGEGLTEAEIVRWLVAEGEPVEADQPIVEVETDKA
                     VVEIPSPYAGIVLSHGGAEGEVIEVGEVLVTIGEPGEVESPKADRDVTEQAARESGSS
                     GTDGGGDVFASPEPIVGTLSEETESLSQSERATSPAPAAVKALPIVRKLARDNGIDLE
                     SVQGTGPGGRITRSDVEAVIGTATGPAVPPVTPAPTTQPAHHEPPVRTHAVGDVAVGA
                     GHGISDAEMAEEPAPRPEGHRKPMSRLRRTIAANMAKSWAEIPHVTTFDDVDATRLLE
                     VRRALGDRHGYKIPIEALVIKAVLPAIDEFPEFNASIEGDDLVMHERRDIGIAVDTPE
                     GLMVAVIHDAAGKSVLDLAAEVRRLGEGARERSLSPNELTGQTFTVSNIGAVGGGHGT
                     PIVPYGTVAILSVGMARLQPIVYEDDLAIAPVMPLSISYDHRVIDGAVGRRFISLVME
                     NLEEPALFLAG"
     gene            complement(5192..>6088)
                     /locus_tag="VFZ80_02400"
     CDS             complement(5192..>6088)
                     /locus_tag="VFZ80_02400"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX6286309.1"
                     /translation="EDIGRTGGVFRITDGLRDKYGEERVFDTPVAESGIVGAALGMAI
                     AGMRPIAELQFLGFSYPAYDQIINHVARIRNRTNHRFTAPMVIRIPYGGGIGAAEHHS
                     ESTEAIYAHIPGVKVVVPSTPRDARALLLAAIDDPDPVVFLEPIRLYRAVKEDLAGFD
                     DDVVIGPLRIERSGQDITLISWGAMMKETRAAAGTLEAEGVSVEVIDVRTLSPLDRDG
                     LVASVEKTGRAVIVHEAPLTGGFGGEIAASITERALYSLRAPVRRVTGWDTIFPLKRS
                     ESHYLPSVDRIVAAASETLENH"
BASE COUNT         1026 a         1973 c         1929 g         1160 t
ORIGIN      
        1 caacgtcgtg cacggccgag gcgagttagc ccatcgcggc gtccacggca gcgatggcgt
       61 ggatccgggt ggtgtcgaac ccagggatga ccccgtcgtc ctcgtccagg atcagagaca
      121 gctcggtgca tccgaggatc gtcccttccg caccctcgtc ggcgaggacg tcgatggcgt
      181 cgacgagcgt ccggtgggac ccgtcatcga cgaccccgaa caccagctcc tcgaagacga
      241 tgcgattgat ctcggctcgg gtctcatccg aaggcacgag gcaatcgatt ccgttctgtg
      301 ccatccggtc ccggtagaac ggctcctcca tgaccgcaga cgtccccaga agtgcggcgg
      361 tctccacccc cgattccttc atgcggtgcg cggtgacgtc gatgatgttt accaccggca
      421 cctcgccgta ggcctcgacg gcgtcggcca ccgaatgggc agcgttggcc gcgatgacca
      481 tcacctcggc gccctgggcc gccagagact tggctgcggg aaccaggatg cgggctagct
      541 ccccccagtc ccgggcgtcg gtgcaggcgg cgatggggtc gaagtcgccg gaccacatca
      601 agacgtccgc cgtatgcttc ccgccgagac gggagttggc ctcctcgttg atgtagcggt
      661 agtagctgat agtcgactgc cagctcatcc cgcccaggat gccgattcgc ttcatgacgt
      721 cgctttcacg aattccctga gatcggcggc gagttgcttc agtgacgcct ggtgcacgta
      781 catcatgtga ccggcctcgt agtaggtcag cttcacgttc tctctcacct ccgggtcgag
      841 acccatgtgg gagaacgtgt actccgttgc gaaatgcggg gtggccaggt cgtagtaccc
      901 attggcgacc aggaccttgg tgccgcggga ccgagacagc agctcgcgga agttctccga
      961 cgtatcgacg tatttgcctt tgaaggtctc gtagtcccag ttcttccaga cctccaaccc
     1021 catgaccttg tagatctcgt cggtttcgaa gccgatctcg tcgcgcaggt agtggttcag
     1081 agcagaggcg gccacgccga tcgtggcgtc gatagatgga tcggcctcga tgacgtttcc
     1141 cgccttgacc cgatcgatgc cggtgtatct ggagtcgatg cgcccgacgg tacggtgctg
     1201 ctcccggagc agttccttgc agaaccgatg gatctggacc cgcaagtcgt agtggtcgac
     1261 gaagtcggcg tcgagcccgg tgtaggcggc gagtcgccct gcgatctcct ttcgcagctc
     1321 gtcgtcgatc tgatcaccct tggcgagcgc ggaccagtac tcccccaggg cgaaatcctc
     1381 cacttcctcg aggaagtctc ggagtggcat ctgctgatac acctcggcga gctgcccgtg
     1441 atatcgggcg gtcgctgcgt aagtgggcag atagagggcg aaggggagat cgttgccggg
     1501 atggatcagc cagttctcgg agtcgatcgc cgaggtctgg tagttgagga tcatcgagat
     1561 gaagatgatc ccgttgaact cgacgttgtg ggccacgaac agctcacggg cgatgcccgc
     1621 gccgcgcaag gtcccgtagc tctccccgga caagtacttg ggcgatgccc atcttccgtt
     1681 gcgattgagg tagtggacga tgaattcgct gaaggcctcg atgtcccgct ggaagtggtg
     1741 gaacttcttc tcatcaccct tcttctcggt gcggctgaag ccggtgccca tggcatcgat
     1801 gcagatgacg tcggccacgt cgaggatcga gtacgcgttg tcgacgagac gtccctgcat
     1861 gcccatcttg aacccatcct catcgaactc catccgcctc ggcccgtaca ggccgagatt
     1921 gagccagacg gtcgatgacc ccgggccgcc gttgaaacag aagagaaggg ggcgatcggc
     1981 cggatcggca tcgtcctggg tgtaggagac atagaagaag gagaccttct ccccattgct
     2041 ctcgaagacc tgcgtgccgg cagttgccgt gtaggcgatc ttggtctttc cgaatttgcc
     2101 ctcgaactgc ttggaggtcc gccgctcctt cagctccgat cccttcttgg ccttcggctt
     2161 cttcttcttc gacttgtcct tcgccattga acggaggcta cgcccttgaa aagggttagc
     2221 actcgagaag gtttgcagcc caattcacac gggctgcgcc tgccgggccg tcctcggtct
     2281 cctcactcct tcggggttcc gtcgtgattg agccactcct cgaggggaac gccaagaccc
     2341 tcagctatgc ggttgatgta gttgaaatag gcgatcacct gggtggcgct ggatatcccc
     2401 tcatcgtcga gccccacctc gcgcagcgcg gagatctcgt cacggcccac cgcggacggg
     2461 gtgagtgtca gcttctcggt gtactcgcag agcgccacgt cgatgcgccc caggccggcg
     2521 ctcctccaat cgacagcgat ccggtcgacg atctcgggat cttcgacctc ggcacggagg
     2581 tcgtggagat gcgccgcgat tcagtagtgg cagtcgttca cctgagaggt gaccacggcc
     2641 atcatctctc gctgtgcccg ggtcagcggc gaagggccgt acatgatcga cagatagata
     2701 cgcatcgaat cgcgcatgac ctccgggagc tggctctgga tcgacacgat gttccagatg
     2761 cggccggcac gtcgcatcgc agcgtcgtac tcgcgacggg cgatcccggt cgcctcttcg
     2821 tagctcagta tgtggacgtg ggacacctca gtactcgccg aacatcacac tgatgctctc
     2881 ccgattgtga atccgctcga tcgcctcggc gaatagggga gccaccgaga cgacctcgac
     2941 tttctcggag atctccacgg gctggttctc caccgtgtcg gtcacgatca gcttctcgat
     3001 cgggctctcg tcgagacgct ccatcgaccc ctgggtgaac accccgtggg tcactgctgc
     3061 gaacaccgac ttcgccccga gacgcatcaa ctcgttggca cacgacacca gggttcccgc
     3121 cgagatggtg aagtcgtcga cgatgaccgt gtgccgccct gagacgtccc ccacgatgtc
     3181 ccagacctcg gcgagctcct cgtggtcctt cctctgcttg tcggcgatgg ccacccccga
     3241 accgagtgcg gtcgcatatc gacgcgcctc ccgggcgaag ccggcgtcgg gggaaaccac
     3301 gacgaggtcg tcacgaccct cggcggcaat gcggtcgcaa aagaccgaga tggcgtacag
     3361 attgtcgacg ggcacgcgaa agaacccctg gatctgaggc gcgtggaggt ccatggccac
     3421 cacgcggtcc gccccggcgg tctcgatggc gtcggcacag acgcgagccc tgatcgacac
     3481 tcgcggctca tccttcttgt cgcccttccc atagctgaag tacgggatga ccaccgtcac
     3541 gttacgggcg ctggctcgct tgaaggcgtc gatccagaag agcaattcca tgaagttgtc
     3601 gttgctgggg aacacggtgg actgcaccag gaacacgtcg tgaccgcgca cgttctgttg
     3661 gaccttgacg aagaggttgc cgtccgagaa gtgccgggtc tcgctggcgc cgagttcgat
     3721 gccgagatgg tcggcgattt tggccgagag acggcggctc ccgctccccg tgaagagcag
     3781 gatcgggtct ggctccgaca tccctgctat ccggcgagga acagggcggg ctcttcgagg
     3841 ttctccatga ccaaggatat gaagcggcga cccaccgccc cgtcgatcac gcgatgatcg
     3901 taagagatgg agagcggcat gaccggggcg atggcgaggt cgtcctcgta gacgattggc
     3961 tgcagtcggg ccatgccgac cgagagaatg gccaccgtcc cgtagggcac gatgggggtg
     4021 ccgtggccgc cgccgacggc tccgatgttg gagacggtga aggtctgccc ggtcagctca
     4081 ttgggggaca gactccgctc cctggccccc tcccccagtc tgcgcacctc ggccgcgagg
     4141 tcgagcacgc tcttgccagc ggcgtcgtgg atcacggcga ccatcagacc ctccggcgta
     4201 tcgactgcga tgccgatgtc ccgacgctcg tgcatgacga ggtcgtcgcc ctcgatgctc
     4261 gcattgaact cggggaactc gtcgatggcg gggagaaccg ccttgatcac gagggcctcg
     4321 atgggaatct tgtagccatg acgatcgccc agagccctgc gcacctcgag gaggcgggta
     4381 gcgtcgacgt cgtcgaaagt ggtgacgtgg gggatctcgg cccaactctt cgccatgttg
     4441 gccgcgatcg tgcgacgcag acgtgacatg ggcttgcgat gtccctcggg gcggggcgcc
     4501 ggttcctccg ccatctccgc gtcggatatc ccgtgccccg caccgacggc gacatcgccg
     4561 accgcgtggg tgcgcaccgg aggctcgtga tgggccggtt gggtggtggg tgccggtgtg
     4621 acgggtggga cggcaggacc tgtggcggtg ccgatgacgg cttccacatc ggatctggtg
     4681 atgcgtcccc ccggcccggt gccttgcacg ctctccaggt cgatcccatt gtcacgagcc
     4741 agcttgcgca cgatgggcag ggccttgacc gcggccggcg ccggggacgt ggcccgctcg
     4801 gactgcgaca ggctctcggt ctcttcactg agggtcccca cgatcggctc cggcgatgca
     4861 aagacatccc cgcccccatc tgtgcccgag gaaccgctct ctcgagccgc ctgctcggtc
     4921 acgtcgcggt cggccttggg ggactcgacc tcgccgggct ccccgatggt caccagcacc
     4981 tccccgacct cgatcacctc accctcggct ccgccatggg agaggacgat gccggcatag
     5041 ggagatggga tttcgacgac tgccttgtcg gtctccactt cgacgatcgg ctggtcggcc
     5101 tccacaggct cgccctcggc cacgagccag cggacgatct cggcctcggt gagaccctcg
     5161 ccgatgtcgg gaagcctgaa ttcgcggggc atcagtggtt ctccagtgtc tcgctcgcag
     5221 ctgccacgat ccggtctacc gagggaaggt agtggctctc cgaccgcttc aacgggaaga
     5281 tcgtgtccca ccccgtcacc cttcgcactg gcgcccgcaa cgagtagagg gcgcgttcgg
     5341 tgatgctggc ggcgatctcc ccgccaaacc ccccagtgag gggagcttcg tgcacgatga
     5401 cggcccgacc ggtcttctcc accgaggcaa cgagcccgtc cctgtcgaga ggtgacagag
     5461 ttcgaacgtc gatcacctcc accgacacgc cctcggcctc gagcgtgccg gccgcggccc
     5521 gggtctcctt catcatcgct ccccagctga tcagggtgat gtcctggcct gagcgctcga
     5581 tgcgaagcgg tccgatgaca acgtcgtcgt cgaagccggc caggtcttcc ttgacggctc
     5641 tgtagagcct tatcggctcg agaaagacca caggatcggg atcgtcgatg gccgccagca
     5701 gcaaagcccg cgcgtcgcgc ggagtcgagg ggacgaccac cttcactccc ggtatatgcg
     5761 cgtagatggc ctctgtcgac tcggaatgat gctcggctgc tccgatcccg ccgccgtacg
     5821 gaatgcgaat caccatcggg gcggtgaatc gatggttggt cctgtttcgg atcctggcga
     5881 cgtgattgat gatctggtcg taggcggggt aggagaagcc gaggaactgg agctcggcga
     5941 tcggcctcat cccggcgatt gccatcccga gtgccgcccc cactatcccc gactccgcca
     6001 ccggcgtgtc gaagacgcgt tcctcgccgt atttgtcccg cagcccgtcg gtgatccgga
     6061 acacgcctcc ggtgcgcccg atgtcctc
//
LOCUS       DASUTA010000119         2193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1334336, whole genome shotgun sequence.
ACCESSION   DASUTA010000119 DASUTA010000000
VERSION     DASUTA010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334336"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="VFZ80_02405"
     CDS             complement(<1..1181)
                     /locus_tag="VFZ80_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6286310.1"
                     /translation="MAMPQCDVMKTSDLGPYPAAPGYEEAVALDAADPLSEYRRSFAH
                     SDLDTIYLNGNSLGRLPVATSELVDEVTTRQWGDQLIRSWNEGWWDLQIRLGDKLAPL
                     IGARPGEVIISDSTSVNLYKLAMAVLSTASSGRRRIVTDDLNFPSDIYILDHVASFHG
                     GELVVVPSDGIHGPVDGLAEAVDGSTALVSLSHTTYTSGYTYDVAAISPMAHGAGAMT
                     LWDCSHSVGAMPIDLVGWGADLAVGCTYKYLNGGPGGPAFLYVRDDLIEVLPSPVPGW
                     WAHASPFEFDLSFTPGPGIRKFHVGTMPILSLAAIEPGLDISLSAGMEAIRHKSVALT
                     TYLIELWQEHLEPLGFRLASPTDPDRRGSHVALRHPMAWPVNRAMIEDVRVVPDYRQP
                     D"
     gene            1225..1299
                     /locus_tag="VFZ80_02410"
     tRNA            1225..1299
                     /locus_tag="VFZ80_02410"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1260..1262,aa:Val,seq:cac)
     gene            complement(1431..1685)
                     /locus_tag="VFZ80_02415"
     CDS             complement(1431..1685)
                     /locus_tag="VFZ80_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286311.1"
                     /translation="MKGRLDRLTQLAYARQRPNAATQELDRRFRLVIADITTALAGTG
                     TSSNSPERRAIIDAVCRRHDFPTLAELEESQRDGHAPDVR"
BASE COUNT          414 a          624 c          728 g          427 t
ORIGIN      
        1 ttgtccggtt ggcggtagtc gggaacgaca cggacgtcct cgatcatcgc tcgattgacc
       61 ggccacgcca tggggtggcg taacgccaca tgagacccgc gccgatcggg gtcggtcggg
      121 ctcgccaacc gaaaacccag aggctcgaga tgttcttgcc agagctcgat caggtaggtg
      181 gtgagggcaa ccgatttgtg tctgatcgcc tccattccgg ccgatagcga gatgtccaac
      241 cctggttcga tggccgccag ggagaggatg ggcatggtgc caacatggaa cttccgaatc
      301 cccgggccgg gagtgaacga gaggtcgaat tcgaaaggcg atgcgtgagc ccaccatccc
      361 ggaaccgggc tggggagcac ctcgatcagg tcgtcacgga catagagaaa cgcaggaccg
      421 cccgggccgc cgttcaggta cttgtaggtg cagcccacgg cgagatcggc tccccagccg
      481 accaggtcga tcggcatcgc cccaacactg tggctgcagt cccaaagggt cattgctcct
      541 gcgccgtggg ccatcggaga gatcgcagct acgtcgtagg tgtatccgga cgtataggtc
      601 gtgtgggaga gcgacacgag tgcggttgac ccatcgacgg cttcggccaa cccatccacg
      661 ggaccgtgaa tcccatcgga ggggaccacg acgagttcgc cgccgtgaaa ggaggcgacg
      721 tggtcgagta tgtagatatc ggaggggaag ttgaggtcgt cggtgacaat gcgacgacgc
      781 cccgaagatg ctgtggacag aacagccatt gcaagcttgt agaggttgac cgaagtcgaa
      841 tcggagatga tgacttcacc gggtctcgcc ccgatgagcg gggccagttt gtctccgagc
      901 cggatctgta gatcccacca gccctcgttc cacgaacgga tcagttggtc accccactgc
      961 cgggtcgtca cctcgtcgac cagctcggac gtcgctaccg gcaggcgacc caaggagttt
     1021 ccattcaggt agatcgtgtc aaggtcgctg tgggcgaacg atcggcggta ttcgctcagt
     1081 ggatcggcgg cgtcgagagc gactgcctcc tcgtaacccg gagccgcggg gtaagggccc
     1141 agatcggagg tcttcatgac gtcgcattgt ggcatcgcca tatcgactag acgttggcgc
     1201 gtcggtaacc tgtggcctcg tcccgggccg ttagctcagt ctggctagag cgctcgcctc
     1261 acacgcgaga ggtcagtggt tcaagtccac tacggcccac ctatccatct cccctgctca
     1321 cagccggaat cttcaatgga cgtagggtgg gcgccagtcg gcgggtccgc tggatggcgg
     1381 aagagccgtg tccgcctcgg cgccgtgcaa gttgcatgtc gctgcggctc tcagcggaca
     1441 tcaggtgcgt ggccgtcccg ctgcgattcc tccagttcgg cgagtgtggg gaaatcgtga
     1501 cgccggcaga cggcatcgat aatggctcgc ctctccggac tgttggaaga ggtccctgtt
     1561 ccggcgagtg cggtggtgat gtctgcgatg actagtcgga agcggcggtc aagctcctga
     1621 gtcgcagcgt tgggcctctg ccgggcatag gcaagctgtg tgagacggtc gagccgacct
     1681 ttcatcgaag ccattgcagg cgcaactcat tgagaatgca tccaaggtcg tgccgacacg
     1741 tcatccgtct caagattcgg cttggtcgat gatcgcttcg actttgggcc aatcgtgggg
     1801 tctccacacc gcaccgccga gcacgtcgag ccagtgtggg cctgaacgtc ggtgagtctg
     1861 ccgtgtgggg tcttgagttc ccggaaaagc actctggagg agccggccgg aaagtgacga
     1921 gcgctgagcc tttgaggggt gtggtgccaa ctgtcctacc agtgctgttc gccctgcagg
     1981 actgattgcg gtctcgtccg ttataagagg cccaatggat ctagtgcgtt tgcccatcac
     2041 tgcggcaaga tcacacgagt gagaggcctt tgatgagaat gcgcccggtc gtattactac
     2101 tagcaagtgt gatggcaggg tgccaggccg ggggtgggga agtagacact cctcagtctt
     2161 cctctacatg ggcttgggag gctaaggctt ggt
//
LOCUS       DASUTA010000120         3332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_945363, whole genome shotgun sequence.
ACCESSION   DASUTA010000120 DASUTA010000000
VERSION     DASUTA010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945363"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VFZ80_02420"
     CDS             complement(<1..262)
                     /locus_tag="VFZ80_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286312.1"
                     /translation="MLRIAVVALEIRDLARASDFSIQARNTAVRYELPILETVANSVY
                     IETLFWGGAWVEAEDLATETIGGHSYADVRLGAVLGALRTRSG"
     gene            complement(286..813)
                     /locus_tag="VFZ80_02425"
     CDS             complement(286..813)
                     /locus_tag="VFZ80_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6286313.1"
                     /translation="MAAINPVDRWTADEKLSIERASFARLGDDEATFLGILAHVPDYA
                     EALWGAMSEALFEGNVDHRLKEMIRIQLARRAGDPYFSKLRSKPALEAGLTEDDIEAA
                     SGDFENDPRFSDAEKWALRYAYLMFRDPEKIDTAFYDQGKKHFTEAQIMELGGLIAIH
                     YGMDVFMRTLQPGEN"
     gene            complement(813..1100)
                     /locus_tag="VFZ80_02430"
     CDS             complement(813..1100)
                     /locus_tag="VFZ80_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286314.1"
                     /translation="MLASAEEIDAAFYERLRAHYTDAEIVELGAFIGFNLGYHTFFRT
                     LDFYPMFTPDGRLVSQEESRRIYGDVVGSHLRPEIQQALARSRAEAEEEVP"
     gene            complement(1233..1478)
                     /locus_tag="VFZ80_02435"
     CDS             complement(1233..1478)
                     /locus_tag="VFZ80_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286315.1"
                     /translation="MPNITMLDIEDLLESELAGFVKKCLKHRAPDPAFHAIQGHNPSL
                     SRALYVAWGTVFNTGAIDHGLKEIIRIQLSRAADCCY"
     gene            complement(1699..2409)
                     /locus_tag="VFZ80_02440"
     CDS             complement(1699..2409)
                     /locus_tag="VFZ80_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286316.1"
                     /translation="MLRVDDLHCSYGMAEIVGGVSFEVTEGEVVAVLGRNGMGKTTLV
                     RAIAGIRPPQVTGGTISYYDQRIDQLASFEVARNGIGYVPQGRHIFGSLTVVENLEVI
                     ARQPPNGAEAWTVGRVFEFFPRLGERRASYGRNLSGGEQQMLAIGRALMTNPSLLLMD
                     EPSEGLAPRVLSAIRERLLELKGPNLSLLLVEQNLGLALSLADRIVILGPPGRVVWSG
                     TSEELEAFDDVKQEHLGV"
     gene            complement(2409..3134)
                     /locus_tag="VFZ80_02445"
     CDS             complement(2409..3134)
                     /locus_tag="VFZ80_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286317.1"
                     /translation="MDEVSVFFGGVVAVNGVSLRVDRGSRWAVIGPNGAGKTTLFRTI
                     AGEVHPSQGRVYLFGEDTSRAQPYTKAQRGLARTYQVTNLFLALSVEENLAIAAQARS
                     PSRFACWRPLRMNGWIDERITRVLESVDLTHRRHDVVSELSHGEQRQLEIGVTLATDP
                     ELILLDEPAAGLSAAERLMMADLIGSLSRDLSLVLIEHDMNIALELVDRVLVLDNGNP
                     IAEGDPEEIRKNQMVQDVYLRGD"
BASE COUNT          590 a         1069 c         1033 g          640 t
ORIGIN      
        1 ggccgctccg ggtacggaga gcaccgagga cggctccgag gcggacgtcg gcataggagt
       61 gccccccgat cgtctccgtt gccagatcct cggcctcgac ccatgcccca ccccagaata
      121 gggtctcgat gtacaccgag ttggccaccg tctcgagaat tggcagctca tatcgaactg
      181 ctgtgtttcg ggcttggatc gagaagtccg acgcccgagc caggtccctt atctcgagcg
      241 ctacaaccgc aatgcggagc aaggtcgaat acggcgcttc gaggctcagt tctcaccagg
      301 ctgcagggta cgcatgaaga cgtccatgcc atagtggatg gcgatgaggc cgccgagctc
      361 catgatctgg gcttcggtga agtgcttctt accttggtcg tagaaggcgg tgtcgatctt
      421 ctcggggtcc cggaacatga ggtaggcgta gcggagcgcc catttctcag cgtcgctgaa
      481 acgggggtcg ttctcgaaat ccccgcttgc ggcctctatg tcgtcttcgg tgagcccggc
      541 ttcgagggcc ggtttcgaac ggagcttcga gaaatagggg tcgccggctc tgcgggccag
      601 ctggatgcgg atcatctcct tcagccgatg gtcgacgttg ccctcgaaga gggcttcgga
      661 catcgcgccc cacagcgcct ccgcgtagtc ggggacgtgg gcgaggatcc ccaggaacgt
      721 ggcctcgtcg tctcctaaac gcgcgaacga ggctcgctcg atggagagct tctcgtcggc
      781 ggtccaccgg tcgaccgggt tgatggcggc catcacggca cctcctcttc ggcctccgcc
      841 cgggaccggg ccagcgcctg ttggatctcg ggacggagat gggatcccac cacgtcgccg
      901 tagatgcggc gcgactcctc ctgggagacc aggcgcccgt ccggggtgaa catggggtag
      961 aagtcgagtg tgcgaaagaa ggtgtgataa ccgaggttga acccgatgaa cgcacccagt
     1021 tcgacgatct cggcatccgt gtagtgcgcc cggagacgct cgtagaaggc ggcgtcaatc
     1081 tcctcggcgg aggcaagcat cagctccgag tattcgagtg cgatcttctc ggcgggagtg
     1141 aagctggggc tgtcgcgcca atggtcgatt ccctcgtcga tacgctcctc ggtgagtccg
     1201 ttccgcttcg ccgaagcgga tctgacgttt cctcaatagc agcagtcagc cgcccgggac
     1261 agctgtatcc gaatgatctc tttgaggccg tggtcgatgg cgccggtgtt gaacacggtg
     1321 ccccaggcca cataaagcgc cctggagagc gacgggttgt gcccctggat ggcgtgaaag
     1381 gcaggatcgg gagcccgatg cttcaggcat ttcttcacaa agccggccag ctccgactcc
     1441 agcaggtctt cgatatcgag catggtgata ttcggcatcc ggcgcctttc ccaccggact
     1501 cacaactcgt cgtgcggtcc ggtctggtga atctgatcgc agatcggtga ttctttcgac
     1561 aatgagatgc cgtcgattct aggaccttac ggctggcgat cacttcgctc gagcggcgcc
     1621 cacatgctct tcccacccct ccagcgggag ccgtcgtccc ctctcagctc gccgctgctg
     1681 gaagactcag cacgaggatt agacgcccag atgctcctgc ttcacgtcgt cgaaggcttc
     1741 tagttcttca gacgtaccac tccataccac ccgacccggt gggccgagga tgacgattcg
     1801 atcggcgagg gacagagcca ggcccaggtt ctgttccacc aagagcaggg acaggttggg
     1861 tcccttgagt tccaacagcc tctccctgat ggcggagagg accctgggag cgaggccctc
     1921 agagggctcg tccatgagca gaaggctcgg gttggtcatc agggcccggc cgatggccag
     1981 catctgctgc tcgcccccgg agaggttccg tccgtaactg gccctcctct cccccaatcg
     2041 gggaaagaac tcgaacaccc tgcccactgt ccacgcttcg gcgccgttgg ggggctgacg
     2101 ggcgatcacc tccaggttct ccacgacagt caggctgccg aagatgtggc ggccttgcgg
     2161 cacatagccg atcccgttac gggcaacctc gaaactcgcc agctggtcga tcctctggtc
     2221 gtagtacgag atcgtgcccc ccgtcacctg tggagggcgg ataccagcga tggcccgcac
     2281 gagggtggtc ttgcccattc cgttgcgtcc cagtaccgct accacctctc cctccgtcac
     2341 ctcgaaagag acaccgccca cgatctcggc cattccgtag ctgcagtgga gatcgtcgac
     2401 gcgtagcatc agtcgcccct caggtagacg tcctgcacca tctggttctt gcggatctct
     2461 tcggggtctc cctccgcgat agggttgccg ttgtcgagta ccagcacccg atccaccagt
     2521 tccagagcga tgttcatgtc gtgctcgata agaacgagag acagatcgcg cgagagcgac
     2581 ccgatgaggt cggccatcat cagccgctcg gctgccgaca gacccgccgc cggctcgtcc
     2641 aggaggatca gctctggatc ggttgccagg gtcaccccga tctctagctg gcgttgctcg
     2701 ccgtgagaga gctccgacac gacgtcgtga cggcgatggg tgaggtccac cgactccagg
     2761 acgcgggtga ttcgctcgtc gatccatccg ttcatccgca gtggacgcca gcatgcgaag
     2821 cggctcggcg agcgggcctg ggcggcgatg gcgagattct cctcgacact gagggccaag
     2881 aacaggttgg tcacctgata cgtccgcgcc aagccccgct gagcctttgt gtagggctgg
     2941 gcccgggagg tgtcctcgcc gaagaggtac acccggccct ggctcgggtg tacctctccg
     3001 gcaatggtcc ggaacaacgt ggtcttgcct gccccgttgg gaccgatcac cgcccaccgg
     3061 ctaccgcggt cgacccggag agatactccg ttcacggcca cgacaccgcc gaagaagacg
     3121 gaaacctcat ccacttccag gaccgttgcg accacgggtt ttctctcttt cagccgccgg
     3181 cgttgccctg gaagtcgcgg ctatacaccg gctgggagag gtactcctcc gcctcgtacg
     3241 gccagaactg atcgatgtcc tcgtaggtct cgatcggcac gttccagaac cgaccgtcgt
     3301 cgcgcatctg cacctcgcgg atatagatgt cg
//
LOCUS       DASUTA010000121         3668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_168756, whole genome shotgun sequence.
ACCESSION   DASUTA010000121 DASUTA010000000
VERSION     DASUTA010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168756"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..996)
                     /locus_tag="VFZ80_02450"
     CDS             complement(124..996)
                     /locus_tag="VFZ80_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005309245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6286318.1"
                     /translation="MTTTGELTTRELKAPGATITYDIRPNSSSIEPILFLIGSPMGAA
                     GFGSLASHFADRTILTYDPRGSERSEKDDPARPSDPETHADDLRRVIEAAGGEPVDMF
                     ASSGGAVNALALVARRPELVRTLVAHEPPLAAILGDREAALTVTQSVNETYMQKGFGA
                     GMAHFLAVTMHEGPFTHEGVTAPDPSMFGLPGEDDGTRTDVLLGQNMVSCTHYEPDFE
                     ALERASTRIVVAAGEESGGQMARRAAHALAERLGLDVVLFPSHHGGFLGGEYGQAGDP
                     DAFAAKLREVLAAG"
     gene            complement(1019..1522)
                     /locus_tag="VFZ80_02455"
     CDS             complement(1019..1522)
                     /locus_tag="VFZ80_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX6286319.1"
                     /translation="MTVTAVQKDPDSLTMTVEAEFEATPGRVWRLWADPRQLERWWGP
                     PTYPATFTAHDLTPGGRVEYHMTGPEGDQPRGYWDIMEVDEPHRLVFRDGFADEDGAP
                     VEDMPVMTATVTIESIDGLRTRMSIASVFPDVQSMEQVLAMGMEEGLTQAMGQIDGIL
                     AEEDVTA"
     gene            complement(1525..1854)
                     /locus_tag="VFZ80_02460"
     CDS             complement(1525..1854)
                     /locus_tag="VFZ80_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019482911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6286320.1"
                     /translation="MQTTLDFDQVFAALADGTRRDIVRRAIAGDEGVSELSEHYPMSF
                     AAVQKHVAILERAGLVTKHRRGRRKVVHTNVAALALARRLLDRYEELWQGRIERMSQI
                     INEIEEE"
     gene            1925..2806
                     /locus_tag="VFZ80_02465"
     CDS             1925..2806
                     /locus_tag="VFZ80_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014692270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX6286321.1"
                     /translation="MRIATWNVNSLSARLPAVEQWLERAAPDVLLMQETKLADEQVPD
                     LAFRMAGYELVHHGEGRWNGVAIASRQELGDVVTNFGEGPVRDSRSRGTDDEGFAPLK
                     EARMVSATTSGIRFVSLYAPNGRQVGSVFYEGKLQWFERLRRWLAENCSPDEPLVLAG
                     DFNVTPGDDDVWDARAVHGGTHVSEPERAALESLRDWGLIDAYRLHQPAPGRYTWWDY
                     RAGMFHKNLGMRLDLIYVTRSLADRTIWAEIDRVARKGKPTPSDHAPVLVDIDQPGIP
                     IDADWEGALERIARRES"
     gene            2868..3563
                     /locus_tag="VFZ80_02470"
     CDS             2868..3563
                     /locus_tag="VFZ80_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286322.1"
                     /translation="MSRLGMPDIVPVLSRGKHRSPRSGACFMEYASFLAGERWSDHPK
                     CTHPLLAGVARDINDHISDDGRATLVPLIPSVIGLNSDDPLLDVIISTRCALLALPVA
                     AEYRQRALAAGLLAARRVIDELSRQPAPRAITGLLAEIEQALAATPHATKWAEGFVAD
                     TVTSTRVFCRRSAPNIVHVAVEGIAEACVRDPASLLADLLTQVIAECRSWIEYESQPK
                     EPTRLHRRGAEVS"
BASE COUNT          636 a         1213 c         1138 g          681 t
ORIGIN      
        1 cccgccgggt cgggccctca tccgggtagt ggcggtcaga ccccatgagg aactcaccac
       61 ccgcaattcg gacctgctcg gccatccaga gatggagatt agagctgtgc gccgcgtgag
      121 ctgtcagccc gcggcgagca cctcgcgcaa cttggctgcg aaggcatcgg gatcaccggc
      181 ctggccgtac tccccaccga ggaagccacc gtggtggctg gggaacaaca ccacgtctag
      241 gcccagccgc tcagcgagtg cgtgggcggc ccttcgtgcc atctgcccgc cggactcctc
      301 tcccgccgcc accacgatcc gggtcgatgc ccgctcgaga gcctcgaagt ccggctcgta
      361 gtgggtgcag gacaccatgt tctggcccaa caggacatcg gttcgagtgc cgtcgtcctc
      421 tcccggcagt ccgaacatgg aggggtcagg cgccgtaacg ccctcgtggg tgaaaggtcc
      481 ctcgtgcatg gtgacggcga ggaagtgagc catgccggcc ccaaagccct tctgcatata
      541 ggtctcgttc acggattggg tgaccgtcag cgccgcctcc ctgtcgccca ggatcgccgc
      601 cagtggaggc tcgtgtgcca ccagcgtccg caccagttcg ggtcgccgcg caaccagggc
      661 gagggcattg accgccccgc cgctgctggc gaacatgtcg acaggctcgc caccggccgc
      721 ctcgatcaca cgacgaaggt cgtcggcgtg cgtttccggg tcggacgggc gggccgggtc
      781 gtctttctca ctgcgctcac tcccccgtgg gtcgtacgtc aggatcgtgc gatcggcgaa
      841 gtgggatgcg agcgacccga atcccgcagc ccccatcggc gagccgatga ggaacaggat
      901 cggctcgatg ctcgagctgt tgggtcggat gtcgtaggtg atagtggcgc cgggagcctt
      961 cagctcacgg gtggtgagct ctcctgtagt ggtcatcagt tcctcctgat tgcactgcct
     1021 aagcagtgac gtcttcttcg gcgaggatgc cgtcgatctg tcccatcgcc tgggtgagac
     1081 cttcttccat gcccatggcc agaacctgtt ccatcgactg aacatccgga aagacgctgg
     1141 cgatcgacat ccgggtgcga aggccgtcga tgctctcgat ggtcacggtg gccgtcatca
     1201 cgggcatgtc ctccacgggg gcaccgtcct cgtcggcgaa accgtcacgg aataccagac
     1261 gatgtggctc gtccacctcc atgatgtccc aatagccgcg gggctggtcg ccctctggcc
     1321 cggtcatgtg atactcgacc ctgcctccgg gggtcagatc atgggcagtg aatgtggccg
     1381 gatatgtcgg cgggccccac cagcgctcca gctgtctcgg gtccgcccac agcctccaca
     1441 cccgtcccgg ggtggcctcg aactcggctt cgacggtcat ggtcagcgaa tccggatcct
     1501 tctgcacggc ggtgactgtc atcgtcactc ctcctctatc tcgttgatga tctggctcat
     1561 gcgctcgatt cggccctgcc acaactcctc gtaacgatcg aggagccgac gggcgagagc
     1621 gagggccgcc acattggtgt ggaccacctt gcgacggccc ctcctgtgct tggtgacgag
     1681 cccggccctt tccaagatgg ctacgtgttt ctgcactgca gcgaagctca tcgggtaatg
     1741 ctcggagagc tcactcactc cctcgtcgcc ggctatggct cgacgcacga tgtcgcgtct
     1801 ggtgccgtcg gccaaggccg cgaacacctg gtcgaaatcg agtgtcgttt gcatagttaa
     1861 tacaaccata tggttgtatc agcgagaagt caatacaccc gaagccaggt agcttcagcc
     1921 cccaatgagg atcgctacct ggaatgtcaa ctcactgtcg gcccgtctcc ccgccgtcga
     1981 gcaatggctg gagcgagcgg ccccggatgt gctcttgatg caggaaacca aattggcgga
     2041 tgagcaagta cccgacttgg cgtttcgcat ggccggctat gagttggtcc atcacggaga
     2101 gggaagatgg aacggtgtcg ccatcgccag ccgacaggag ctgggcgatg tggttaccaa
     2161 tttcggcgag ggcccggtgc gtgacagtcg ctcgagaggg accgacgacg agggattcgc
     2221 cccgttgaaa gaggcacgaa tggtcagcgc caccacctcg ggtatccgat tcgtcagcct
     2281 gtatgccccc aatgggcgtc aggtcgggtc cgtcttctac gaaggaaagc tgcagtggtt
     2341 cgagcgtctc cgtcgatggc tcgccgagaa ctgctcaccc gacgagcctc tggtgctggc
     2401 aggtgacttc aatgtgactc ctggcgacga cgacgtctgg gacgcccggg ccgtccacgg
     2461 aggcactcat gtctccgaac cggaacgggc tgcgctggaa agcctccgag attgggggct
     2521 catcgacgcc tatcgactcc atcagcccgc tccgggccgc tacacgtggt gggactaccg
     2581 ggccggcatg ttccacaaga acttggggat gcgtctcgac ctcatctacg tgacccgaag
     2641 cctggccgac aggacgatct gggccgagat cgatcgggtt gcccgcaagg gcaagccgac
     2701 gccatctgat cacgccccgg tgctcgtcga catcgaccag cccggcattc ccatcgatgc
     2761 cgattgggag ggagccctcg agcgaatcgc ccgccgtgag agctgacgtc aagagctcta
     2821 acggcctttg tgagtaagga ccgtcgcgat ggggtatgtt tggcgtcatg agccggctcg
     2881 ggatgcctga catcgtcccg gtgctgtcga ggggcaagca ccggagccct cgctccgggg
     2941 cgtgtttcat ggagtacgcc tcattcctcg ccggcgagcg gtggagcgat catccgaagt
     3001 gcacccatcc ccttctcgcc ggagtcgcca gagacatcaa cgatcacatc tccgatgacg
     3061 ggcgggccac gctcgtcccg ctcatccctt cggtcatcgg gctgaacagc gacgaccctc
     3121 tcctcgacgt catcatctcg acacgatgtg ctctgctggc tctgccggta gccgccgaat
     3181 atcgacaacg agcattggcc gcgggtctcc tcgccgcacg ccgggtgatc gacgagctga
     3241 gccggcagcc tgcccctcgt gccatcaccg gtctcctggc ggaaatcgag caggcgctgg
     3301 ccgcgacacc tcacgcaacc aaatgggccg aaggcttcgt cgcggacacc gttacctcga
     3361 cacgagtctt ctgccgtcgg tccgctccca acatcgttca cgttgcggtc gaaggcattg
     3421 ccgaggcctg cgttcgcgat cccgcctcac ttctggccga cctcctgacc caggtcatcg
     3481 ccgagtgccg gtcgtggatc gaatacgagt cgcagccgaa agagcccacc cggctgcacc
     3541 gtcgaggagc tgaggtctcc tagctcccgg gcttcgacgt cccaactgac gacagggcct
     3601 ccgtcacctg ttcgacgcca aaggcgaagg cttcgtcgac gtcactctcc atgcggaatg
     3661 agcctgcc
//
LOCUS       DASUTA010000122         4342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_945477, whole genome shotgun sequence.
ACCESSION   DASUTA010000122 DASUTA010000000
VERSION     DASUTA010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945477"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VFZ80_02475"
     CDS             complement(<1..637)
                     /locus_tag="VFZ80_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6286323.1"
                     /translation="MQTTANVSTLVDANLATGREAKTALITADDEDLTYADLHAAVNR
                     VAARLRDLGIRREERVLVVLDDTPAFPATFLGAMRIGAVPIPVNFLARPQDFGYFLDD
                     SYAVAGVIDSVFLGKVGPEIAQRPHVRLIVANGDTAEGESLDAWLVEEADEVDPVDTH
                     PDDMAFWLYSSGSTGPPKGVVHRHANVWATCQNYAIPTLGISEDDTVYSSTP"
     gene            complement(687..2129)
                     /gene="boxB"
                     /locus_tag="VFZ80_02480"
     CDS             complement(687..2129)
                     /gene="boxB"
                     /locus_tag="VFZ80_02480"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014994511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEX6286324.1"
                     /translation="MTWQDVDYDSLIPNNVGLAEDPKLQRALEQWHPGYIDWWMSMGP
                     EGFQMADVYLRTAISVEKEGWAKFGFVKMPEYRWGILLAPEEEERKIPFGAHVGEPVW
                     QEVPGEYRSMLRRLIVIQGDTEPASVEQQKHLGSIAPSLYDMRNLFQVNVEEGRHLWA
                     MVYLLHKYFGRDGRAEADDLLKRSSGSEERPRMLGAFNEETPDWLSFFMFTYFTDRDG
                     KMQLEALAQSGFDPLSRTCRFMLAEEAHHMFVGYTGVMRVVEATARAMAKAGIDDPHD
                     TDAVRRLGVIDLPAIQRKVNLHYSLTLDLFGSEESTNAANFFNSGLKGRYHEDRLEDD
                     HRLRDSTYPVMKLVDGELGQVEIPALNALNARLRDDFMEDCLGGIRRWNKEIASHGVD
                     FEITLPHVAFNREIGEFAALDVSPTGEVITGEQFEASRDDWLPTADDQAYIESIMQPH
                     YEPGVFAPWIAPPTRGIDQKPPDFEYVKLP"
     gene            complement(2129..3778)
                     /gene="boxC"
                     /locus_tag="VFZ80_02485"
     CDS             complement(2129..3778)
                     /gene="boxC"
                     /locus_tag="VFZ80_02485"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HEX6286325.1"
                     /translation="MIDFQTHPDSYRHWKLRVEGTVAYLDMEVDPDGGLNPGYELKLN
                     SYDLGVDIELADAVQRLRFEHPEVGAVVIGSRLDRMFCAGANIGMLASASHPLKVNFC
                     KFTNETRNAIEDASRNSGQRYIAAIGGNAAGGGYELALAADHLMLIDDRNAAVSLPEV
                     PLLGVLPGTGGLTRLTDKRHVRRDRADVFCTLEEGIKGSKALDWGLVDELVPAPGFDE
                     TVAERAMELATRTDRPSEAAGVPLTPLDREIGDEEIRYSTVRVDLDRADRTASLTILG
                     PDDSPPVNGDEAVSAGSSFWPLRLARELDDAFLHLRANELEIGSITIHSRGDLALVLA
                     YDTFLLSQADHWFVREVLLYWARVMRRVDLTARTIIALVEPESCFGGFLAELTLAADR
                     SYMLIGERFGDESGIAHIVFTEANRFGFPMSNGLSRLETRFWGDADGLATAHGVIGEK
                     LDAESAVAAGVVTDAMDDLDWDDELRIIIEERSAFSPDGLTGMEANLRFPGPETMETK
                     IFGRLTAWQNWVFQRPNASGETGALRRYGTGLRPDYDHRRV"
     gene            3853..>4342
                     /locus_tag="VFZ80_02490"
     CDS             3853..>4342
                     /locus_tag="VFZ80_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX6286326.1"
                     /translation="MQFHLNGFRPGDPRIAEPAEGRESSPGPLPEELDVLIVGCGPAG
                     LTLAAQLAQFPEINARIVEMKEGRLLKGQADGIAVRTVEVFEALGFSERVLKEAYWVN
                     ETSFWKPDDAEPEVIVRSGRIQDTEDGLSEFPHVILNQARVHDFLLETMANSPTRMVP
                     DYA"
BASE COUNT          776 a         1333 c         1410 g          823 t
ORIGIN      
        1 tcggggtact ggaatagaca gtgtcgtctt cgctgatgcc gagtgtgggt atcgcgtagt
       61 tctgacaggt ggcccagacg ttggcgtgac gatgaaccac gcccttgggt gggcccgtcg
      121 agcccgagct gtacagccag aaggccatgt cgtctggatg ggtgtcgacc ggatcgacct
      181 catcggcctc ctcgacgagc caggcgtcga gcgattcccc ctcagccgtg tccccgttgg
      241 ccacgatcag tcgcacgtgg ggtcgttggg cgatctcagg accgaccttc cccaggaaga
      301 ccgagtcgat cacgccggcc actgcatagg aatcgtcgag gaaatacccg aagtcctggg
      361 gccgggcaag gaagttgacc gggatgggca ctgccccgat gcgcatcgcg ccgaggaagg
      421 tcgccgggaa ggcaggggtg tcgtcgagga cgaccaggac ccgttcttcg cgtctgatgc
      481 ccagatcgcg aagccgggcc gccacccggt tcaccgcggc gtggaggtcg gcgtaggtca
      541 ggtcttcgtc gtcggcggtg atcagggcgg tcttcgcctc acgccccgtc gcgaggttgg
      601 cgtcgacgag ggtgctgacg ttggccgtgg tctgcatgtc agtcgacgct agttaggggg
      661 ctcacggacg agtcggggcg attgcgtcat ggcagcttca cgtactcgaa gtctggcggc
      721 ttctgatcga tcccgcgggt cgggggtgcg atccaaggag caaacacacc gggctcgtaa
      781 tggggctgca tgatgctctc gatgtatgcc tggtcatcgg cggtgggcag ccaatcatct
      841 cgagaagcct cgaactgctc cccagttatc acttcgccgg ttggcgacac gtcgagggcg
      901 gcgaactctc ctatctcacg gttgaacgcg acgtggggca atgtgatctc gaagtcgacg
      961 ccatgactgg cgatctcctt gttccagcgt ctgataccgc cgagacagtc ctccatgaag
     1021 tcgtcacgga gcctggcgtt caaagcgttc agcgccggga tctcgacctg gcccagctca
     1081 ccatccacca gtttcatcac cgggtaggtc gagtcgcgaa gacggtggtc gtcttcgagg
     1141 cggtcctcgt ggtagcggcc cttgagcccg gagttgaaga agttggcggc gttggttgac
     1201 tcctcggatc cgaacaagtc gagagtcaac gagtagtgca gattcacctt gcgctggatg
     1261 gcggggagat cgatcacgcc gaggcgtcgg accgcatcgg tgtcatgcgg gtcgtcgata
     1321 cccgccttcg ccatcgccct ggccgtcgct tcgacaacgc gcatcacacc ggtgtagccg
     1381 acgaacatgt gatgggcctc ttcggcgagc atgaagcggc aggttcggga caacggatcg
     1441 aaacccgatt gggccaatgc ctcgagctgc atcttcccgt ctcgatcggt gaagtaggtg
     1501 aacatgaaga aggacagcca gtcgggtgtc tcctcgttga aggccccgag cattcgcggt
     1561 cgctcctcac tgccggatga gcgtttcaag aggtcgtcgg cctccgcgcg tccgtcacgc
     1621 ccgaagtact tgtggagcag gtagaccatc gcccacaggt gccgaccctc ttcgacattc
     1681 acctggaaca ggtttctcat gtcgtacaga gaaggggcga tgctccccag atgcttttgt
     1741 tgctcgaccg aagccggctc ggtgtcaccc tggatgacga tcagtcgtcg cagcatggac
     1801 cggtactcgc cagggacctc ctgccacacc ggttccccga cgtgggctcc gaacgggatc
     1861 ttgcgttcct cctcttccgg cgccaacagg attccccagc ggtattcggg catcttcacg
     1921 aagccgaact tcgcccagcc ctccttctcg acactgatcg cggtgcgcag atacacgtct
     1981 gccatttgga atccctcggg gcccatcgac atccaccagt cgatgtagcc tgggtgccat
     2041 tgctcgaggg cgcgttgcag tttggggtcc tcggccagcc cgacgttgtt ggggatgagg
     2101 ctgtcgtaat cgacgtcttg ccaggtcatc agactctcct gtggtcgtaa tcgggacgta
     2161 gtcctgtgcc gtaccggcga agggccccgg tctcacccga ggcattggga cgctgaaaca
     2221 cccagttctg ccaggcggtg agacgtccga agatcttcgt ttccatggtc tcggggccgg
     2281 ggaagcggag gtttgcctcc attccggtca aaccgtcggg ggagaacgcg gagcgctcct
     2341 cgatgatgat gcgaagctcg tcgtcccagt cgagatcatc catggcatcg gtgacgactc
     2401 ctgcggcaac cgcggactct gcgtcgagct tctcaccgat gacgccgtgt gcagtcgcca
     2461 aaccgtccgc atctccccag aagcgggtct ccagccggga gaggccgttc gacatcggga
     2521 aaccgaatcg gttggcttcc gtgaagacga tgtgggcgat gccgctctcg tctccgaatc
     2581 gctcaccgat gagcatgtag gaacggtcgg cagcgagagt gagctcggcc agaaagcccc
     2641 cgaagcaaga ttccggctcg accagggcga tgatcgtccg agcggtgaga tcgacccgcc
     2701 tcatgactcg ggcccagtag agaaggactt cccgcacgaa ccaatggtcg gcctgactga
     2761 ggagaaaagt gtcgtatgcc aggacgaggg cgaggtcgcc ccgggaatga atcgtgatcg
     2821 agccgatctc caattcgttt gcccggaggt gtaggaaggc gtcatcgagc tcgcgtgcca
     2881 gcctcaaggg ccagaacgag ctgccggccg agacggcctc gtctccgttg accggtggcg
     2941 agtcgtccgg cccgaggatc gtcagggagg cggttctgtc cgcccggtcg aggtcgaccc
     3001 ggaccgtcga gtagcggatc tcctcgtccc cgatctcccg gtcgagcggc gtgagcggca
     3061 caccggcggc ctccgatggg cgatcggtcc gggttgccag ctccatggcc cgctcggcga
     3121 ctgtctcatc gaaccccgga gccggcacca actcatcgac caagccccag tccagggcct
     3181 tcgatccctt gatgccttcc tcgagggtgc agaagacgtc ggcacggtcg cgacggacgt
     3241 ggcgcttgtc ggtgagacga gtgaggcccc ctgttcccgg aaggacgccc agcagcggca
     3301 cctcgggcaa tgacaccgcg gcattgcggt cgtcgatcag catcagatga tccgccgcca
     3361 gcgccagctc gtagcctccg cctgctgcgt tgcccccgat ggcggcgatg taccgctggc
     3421 cggagttgcg tgatgcatct tcgatggcgt tgcgggtctc gtttgtgaac ttgcagaagt
     3481 tgaccttcag agggtgggag gcagacgcca gcatgccgat gttggcgccg gcgcagaaca
     3541 tgcggtcgag tctggagccg atgaccaccg ctcccacctc ggggtgctcg aagcggagcc
     3601 gttgcacggc atcggcgagc tctatgtcga ctcccaggtc gtacgagttg agcttgagct
     3661 cgtatcccgg gttcagccca ccgtcggggt cgacttccat gtcgaggtag gccaccgtcc
     3721 cctcgactcg gagcttccag tgtcgatagg agtcggggtg tgtctggaag tcgatcacaa
     3781 gccgataata ggctgaccgc ctggtcagga gcaaccgatc gggaacagtc cgtttcgtac
     3841 tattcactct cgatgcagtt ccacctcaac ggattcagac ccggtgatcc ccgcatcgcc
     3901 gagcccgcgg agggtcgtga atcaagtccc ggtcccctgc ccgaagagct agatgtgctc
     3961 atcgtcggtt gcgggccggc cggtctcaca ctggcggcgc agctggcgca attccccgag
     4021 atcaacgctc gcatcgtcga gatgaaggaa ggccggctgc tgaaaggcca ggcggatggc
     4081 atcgcggtgc ggactgtgga ggtgttcgag gccctcggct tcagcgagcg cgtgctgaag
     4141 gaggcctatt gggtgaacga gacgtcgttc tggaaaccgg acgacgccga acccgaggtg
     4201 atcgtgcgca gtggccggat tcaggacacc gaagacggcc tgagcgagtt tcctcacgtc
     4261 atcctgaacc aagcccgggt ccacgacttc ctgctcgaaa ccatggccaa ctcacccacg
     4321 agaatggtgc cggactatgc ct
//
LOCUS       DASUTA010000123         3008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_426603, whole genome shotgun sequence.
ACCESSION   DASUTA010000123 DASUTA010000000
VERSION     DASUTA010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426603"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..581)
                     /gene="rdgB"
                     /locus_tag="VFZ80_02495"
     CDS             complement(6..581)
                     /gene="rdgB"
                     /locus_tag="VFZ80_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX6286327.1"
                     /translation="MIPRLVVASKNPDKIEEIEDVLTDTGLASEIVRGLEWPDVEETA
                     DSLEENALLKARAVTEATGLPSLADDTGLEVDALDGAPGVETARFAGDGARYEDNVKK
                     LLAVLEGRSDRTARFRTVVALVFPDGVEVTADGTLDGVIAATARGTEGFGYDPVFEVN
                     GKTLAEMSAEEKNDRSHRARAIRALAESLGL"
     gene            complement(578..1279)
                     /gene="rph"
                     /locus_tag="VFZ80_02500"
     CDS             complement(578..1279)
                     /gene="rph"
                     /locus_tag="VFZ80_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005294109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX6286328.1"
                     /translation="MPERSPNELRPVSVELGFTESTPGSVLISMGRTRVLCTASVEDD
                     VPRWMRDSNKGWVTAEYAMLPGSSPERVRRDHVSGGRAKEISRLIGRALRAAVNLKAM
                     GEAMVRVDCDVIEADGGTRTAAVTGAWIALHQALEDAVDREVMNANPIIDQVAAVSVG
                     IVDGAKLLDLDYEKDVKAEVDLNVVMSGRGLLVDVQGTAEGAPFTREDLDELLDLATG
                     GIEELHDLQRRAVEA"
     gene            complement(1303..2031)
                     /locus_tag="VFZ80_02505"
     CDS             complement(1303..2031)
                     /locus_tag="VFZ80_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6286329.1"
                     /translation="MRVHVVGSSGTFPSPGHPGSGYVIEHEGTRVWCDAGPGTFMSLP
                     MDTDLIDAVVVSHQHPDHCSDVFAAYHAWTYRPEPRDPVPLFAPQAVWDRLVGFIEKE
                     PTCFDFSPVANGDATAIGSLRVEFGEMDHPVPTVGSRWEGSNRTLFFTADTGPSGSWM
                     RLADGVDVMLSEASLQGETDDKAVPHHLTAAEAGQLARDAGAGRLVLTHIPPYLEPSR
                     SVAEAEKTFDRPVRLAVPGTSFTV"
     gene            complement(2028..2603)
                     /gene="pdxH"
                     /locus_tag="VFZ80_02510"
     CDS             complement(2028..2603)
                     /gene="pdxH"
                     /locus_tag="VFZ80_02510"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX6286330.1"
                     /translation="MASDPISEFESWFGDVTTTAIEEPNAFVLATVDGDGAPSARALL
                     MKDLSDDGISFYTGMDSRKSRALRANPRAAATFVWIPLHRQVRFEGVVTEIDGDVADA
                     YFATRPRGAQLAAHASHQSELVADRETLDRAFTQLESELGDVIPRPEHWGGWLLTPTA
                     VEFWQGRPNRFHDRILYRLEEGAWVKERLSP"
BASE COUNT          534 a          991 c          915 g          568 t
ORIGIN      
        1 cggcctcaca gacccagcga ctcggccagc gctcttatcg cacgggctcg atgagatcgg
       61 tcgttcttct cctcggccga catctcggcg agtgtcttgc cattgacctc gaacaccggg
      121 tcatacccga agccttcggt ccctcgggcg gtcgcagcga tcaccccatc gagtgtcccg
      181 tcggccgtga cctcgacacc gtctggaaac accaacgcca caaccgtgcg aaagcgagct
      241 gtacggtccg agcgaccctc gagaacagcc agcagcttct tgacgttgtc ttcgtagcgc
      301 gccccgtctc cggcaaagcg ggccgtctcc acacccggtg ccccgtcgag cgcgtcgacc
      361 tcgagaccag tgtcgtctgc cagggaagga agacctgtcg cctcagtgac agcgcgggcc
      421 ttcagcagcg cgttctcctc cagcgagtct gccgtctcct cgacgtcggg ccattccaga
      481 ccgcggacga tctcgctggc gaggccggtg tcggtcagca cgtcttcgat ctcctcgatc
      541 ttgtctgggt tcttggaggc aaccacgagc ctcgggatca cgcctccacc gccctgcgct
      601 gcaggtcgtg gagctcttcg attccgccgg tcgccaggtc gagcagctcg tcgaggtcct
      661 ctcgggtgaa tggcgcgcct tcagcagtgc cctgcacatc cacgagcaac ccgcgcccac
      721 tcatgacaac attgaggtcg acctcggctt tgacgtcctt ttcgtagtcg agatcgagca
      781 gtttcgctcc atcgactatg cccaccgaca ccgccgccac ctgatcgatg atggggttgg
      841 cgttcatgac ttcgcgatcc accgcatcct cgagagcttg atgaagcgct atccatgctc
      901 ctgtcacggc tgcagtccgg gtgcctccgt cggcctcgat cacgtcacaa tcgactctga
      961 ccatcgcctc tcccatcgcc ttgaggttca cggcggcgcg gagggcgcgt ccgatgagcc
     1021 gagagatctc cttggcccga ccgccgctga cgtggtcacg gcgaacccgc tccggtgagg
     1081 atccgggaag catggcgtat tccgcggtca cccatccctt gttcgaatct ctcatccacc
     1141 tcggaacatc gtcctccacc gacgccgtac acaggacacg ggtccgaccc atcgagatga
     1201 ggacggagcc gggggtcgat tcggtgaagc ccagttccac gctgacggga cggagctcgt
     1261 tgggagatcg ctcaggcatg gggctgaaac tacttgtgac tgtcaaacag tgaaactcgt
     1321 gcctggaact gccaaccgaa ccggccgatc gaaagtcttc tcggcttccg ccaccgatcg
     1381 ggaaggctcg agataaggcg ggatatgggt gagcacgagc ctgccggccc cggcgtcgcg
     1441 ggctaactga cccgcttccg cagcggtgag atgatgtggg accgccttgt cgtcagtctc
     1501 cccttggagg gaggcctccg agagcatgac atcgaccccg tcggcgagcc gcatccagga
     1561 accagacggt ccggtgtcgg cggtgaagaa cagggttcgg ttgctccctt cccatctcga
     1621 tccaaccgtg ggcaccgggt ggtccatctc accgaattcg acacgaagcg atccgatcgc
     1681 tgtggcgtcg ccattcgcaa ccggggagaa atcgaaacag gttggctctt tctcgatgaa
     1741 ccccacgagc cgatcccaaa ccgcctgcgg agcgaagagg ggcaccgggt cccgtggctc
     1801 agggcggtac gtccaggcgt ggtatgcggc gaacacgtcg gagcaatggt cgggatgttg
     1861 atgagagacg acaaccgcgt cgatgaggtc ggtgtccatc ggaagagaca tgaaggtccc
     1921 cgggcccgca tcgcaccaga cacgggtgcc ctcgtgctcg atgacgtagc cggagcccgg
     1981 gtgaccaggc gaaggaaagg tgcccgacga cccaacaacg tgcaccctca tggcgagaga
     2041 cgctccttca cccacgcgcc ttcctcgagc cgatagagaa tccgatcgtg gaagcggttg
     2101 ggccgcccct gccagaactc gaccgctgtg ggggtgagca gccacccgcc ccagtgctcg
     2161 ggccgtggga tcacatcccc caactctgac tccaactggg tgaaggcacg gtccagagtc
     2221 tcacggtcgg caaccagctc gctctggtgt gaagcgtgtg ccgccagttg agcaccccgg
     2281 ggccgagtgg cgaaataggc gtccgcaacg tcaccatcga tctctgtgac cacaccttcg
     2341 aacctgacct gtcggtgaag gggaatccag acgaatgtgg ccgccgctct cgggttggca
     2401 cggagggccc gactcttgcg gctgtccatc ccggtgtaga aggagattcc atcatcggag
     2461 aggtctttca tgagcagcgc tcttgccgaa ggcgcaccat cgccatcgac ggtggcgagg
     2521 acaaaggcgt tgggttcctc gatggcggtg gtggtgacgt ctccgaacca cgattcgaac
     2581 tcgctgatcg ggtcggaggc catcgagtcc tctgtcagac cctcggtttc gtactcccgg
     2641 cgcaagcgcg ccgcgacatc tcctatcacc ctgcgacgat aacctcgccc ggtgacagcc
     2701 gatccctctg cttcgctcgg cacaatggtt ggtcgtgcct tgagacgtcg ttgccccagg
     2761 tgtgggcaac cggcgttcga ctcctggttc aagctcagag agacgtgtcc ggcgtgcggt
     2821 cttcgtttcg agagagagcc cggctactgg gttggggcgg tgacgatcaa caccgtcgtc
     2881 atcttcgcca cgttcctcct ggtcttcggt ggatcggtgc tgctcacgtg gccggaggtg
     2941 ccctggaccg gcgtcctgat cgtcacactc atggtcaatc tcttggttcc gacggtcttc
     3001 tatcccat
//
LOCUS       DASUTA010000124         2594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_557645, whole genome shotgun sequence.
ACCESSION   DASUTA010000124 DASUTA010000000
VERSION     DASUTA010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557645"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VFZ80_02515"
     CDS             <1..848
                     /locus_tag="VFZ80_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286331.1"
                     /translation="DVTDVNRSDFIENRRNLGNALFAIGITGLIAALVVGAAGWILAT
                     RSNSRLADTIKPLGDLVVNVSDSVEATQVIVARTIEAVEGIESATRSAGRTLDSVSGL
                     IDSTSEVLGDDLANGLDDAVSTLPALVDTGRIIDRTMRTLSLVGVDYDPEVPLDESLG
                     SLEESLRPIPDQLRDQVSALGEVGTDIDVVARDAGSLAATLLEARIEMAEARDILVET
                     GENVTRAAASLTSIEGDLETYDLLSKIVVVGVALALLTGAVAPLLIGAHLRRTPDSGE
                     SETLL"
     gene            complement(894..2087)
                     /locus_tag="VFZ80_02520"
     CDS             complement(894..2087)
                     /locus_tag="VFZ80_02520"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX6286332.1"
                     /translation="MKPLVILSVLCAVTGCAGDSADTPDMAQPSTSMLAEGGIELEPL
                     FRTEMDGAVAANVATNGSDPPVVAWITADHVMASTLDLAVGDLVETREVDGGVVPIAH
                     PIERPAVAVRSDGSADIAFTSFQGSGASVFYAVNGSQPVVISGDPRVETNLVHVTLVE
                     DTPVLAWLEASTLSVGITESGTIAERELVDDQTCDCCNPVPAMAGDALVVSYRDFDTV
                     DGEIVRDVVAIASTDGGDSFGEVMSVADDHWFITGCPFSGPSSVVIGNELIVAWMDGR
                     QSVHPDQQTSSIWVDRSSDGGASFGADVEVTGEGLNRWPVMGLDDNGVIHMVWETAGA
                     DGGLSYATSSDDGHTFQVQDLLVTRSSGDGGAPTSPSVAIHEDLLIVTWATSGVGHVG
                     VWRIG"
     gene            2137..>2594
                     /locus_tag="VFZ80_02525"
     CDS             2137..>2594
                     /locus_tag="VFZ80_02525"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX6286333.1"
                     /translation="MTHYQLDQPVGPSLGDWRRPPFPPPSTLRGHHVRVEPLDSGLHG
                     SGLWSSLENAPPETWTYMTFGPFQDYAAFEDALDSIVSPPDWSAYSIVVDEQPLGFAA
                     YLRINPGDGVIEIGSIVFSPALQRTTAATEAIYLLVENVFDLGYRRCEWK"
BASE COUNT          490 a          847 c          789 g          468 t
ORIGIN      
        1 ccgacgtgac cgacgtgaac cgctcggact tcatcgagaa caggcggaat ctcggaaatg
       61 cactcttcgc aatcgggatt acagggctga tagccgccct cgtcgtggga gccgcaggat
      121 ggattctggc cacccgatcc aacagccgat tggccgacac gatcaagcct ttgggcgacc
      181 tcgtggtcaa tgtctccgat tcggtggagg cgacccaggt gatcgtcgcc cgaaccatcg
      241 aggcggtgga ggggatcgag tccgcaacgc gctccgccgg tcgcaccctc gactcggtga
      301 gcggtctcat cgacagtacg tccgaggtgc tcggggacga cctcgccaac ggccttgatg
      361 atgccgtcag caccctcccc gccctcgtcg acaccggtcg catcatcgat cgcacgatgc
      421 gcacactcag cctggtcggg gtcgactacg accccgaagt tcctctcgac gagtcgctgg
      481 gaagcctcga ggagtcgttg cgacccattc cggaccagct cagagaccag gtgtccgcgc
      541 tgggggaggt gggcacggac atcgatgtcg ttgcccgcga tgccgggtca ctcgctgcga
      601 cccttctcga agcgcgcatc gagatggccg aagctcgcga catcctggtc gaaaccggtg
      661 agaacgtgac cagggcagct gcgagcttga cctcgatcga gggcgacctc gagacgtacg
      721 acttgttgtc gaaaatcgtc gtggtcgggg tggctctggc ccttctgacg ggagctgtgg
      781 ctccccttct tatcggagca catctccggc ggacgcctga ctcgggcgaa tctgaaaccc
      841 ttctgtgaag gcgggacggg atctgttccc gccaccgctc cacaaacggg ggatcagccg
      901 atgcgccaaa ccccaacatg cccaacgcct gatgtggccc aggtcacgat gagcaggtct
      961 tcatggatgg cgaccgatgg tgaggtggga gcaccgccgt cacccgacga cctggtcacg
     1021 agcaggtctt ggacctggaa cgtgtggccg tcgtcagaag acgtcgcata ggagagccca
     1081 ccatcggctc ctgcggtctc ccagaccatg tggatgacgc cgttgtcgtc aagacccatc
     1141 acgggccatc ggttcagccc ctcccccgtg acctcgacgt ctgcaccgaa gctcgcaccg
     1201 ccatcactgc tcctatccac ccagatcgac gaagtctgct ggtcggggtg aaccgactgg
     1261 cgaccatcca tccaggcaac gatcaactca ttcccaatca cgaccgagct cggaccggag
     1321 aatgggcagc ccgttatgaa ccaatggtca tcggccacgg acatgacctc tccgaagctg
     1381 tctccgccat cggtcgaagc gatcgcgacc acgtctcgga cgatctcacc atcgacggtg
     1441 tcgaagtccc gatacgagac gacgagggca tcacccgcca tagccggcac cgggttgcag
     1501 cagtcacacg tctggtcgtc gaccagttcc cgctcggcga tagtgccgct ttcggtgatc
     1561 ccgaccgaga gggtagatgc ctccaaccag gcgagcacag gggtgtcctc gaccagagtc
     1621 acgtgaacca gattggtctc caccctcggg tcgccggaga tcacaacggg ttgtgaccca
     1681 ttgacggcat agaagaccga tgcgcccgat ccctggaatg acgtgaaggc gatgtcggcg
     1741 gacccgtcgc tgcggacggc gaccgcgggt cgctcgatgg gatgggcgat ggggactact
     1801 ccaccatcga cctcccgggt ctcgacgagg tcgccaaccg ccaggtcgag tgtcgacgcc
     1861 atcacgtggt cggccgttat ccaggccacc actggagggt ccgatccgtt cgtggccacg
     1921 ttggcagcca cggctccgtc catttcagtc cgaaacaggg gctcgagctc gatccctcct
     1981 tctgcaagca tcgatgtcga aggttgtgcc atatcagggg tgtctgccga atcgccggcg
     2041 cagccggtca ccgcgcacag caccgagagg atgacaagag gcttcactcg ggtcgatcct
     2101 acggcggatc gtgccttccg taggctggcg aacctgatga ctcactacca gctcgatcag
     2161 cctgtcggac cgtcgctagg cgactggaga cggcccccat tcccaccccc atcgacactc
     2221 cggggccacc acgtgagagt ggagccgctg gattccggtt tgcatggctc cgggttgtgg
     2281 agctccctcg agaatgcacc accagagacc tggacctaca tgacattcgg gccgtttcag
     2341 gactacgcag cgttcgaaga cgccctggac tcgatagtgt ccccgcctga ttggtcggcc
     2401 tactccatcg tggtcgatga acagcctctc ggcttcgcgg cttatctgcg gatcaacccc
     2461 ggggatggag tcatcgagat cggctccatc gtcttctccc ctgctctgca acggaccacg
     2521 gcggccaccg aggccatcta cctgttggtc gagaacgtgt tcgatctggg ttaccggcga
     2581 tgcgagtgga agtg
//
LOCUS       DASUTA010000125         5787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_945621, whole genome shotgun sequence.
ACCESSION   DASUTA010000125 DASUTA010000000
VERSION     DASUTA010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945621"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1049)
                     /locus_tag="VFZ80_02530"
     CDS             complement(75..1049)
                     /locus_tag="VFZ80_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6286334.1"
                     /translation="MNEVIRAEGLVKNYGELRALDGLDLSVPQGTVLGLLGPNGAGKT
                     TAVRILTTLLVPDEGSATVAGIDVVANPNAVRSTIGLSGQYAAVDEYLTGFENLYLFG
                     KLYRLGRDKARMRAGELLERFDLIEAGDRPVKTYSGGMRRRLDLAGALVANPTVLFLD
                     EPTTGLDPRGRADMWDVIREMVGAGTTLLLTTQYLEEADRLADEIVVIDHGRAIARGT
                     ADQLKSDIGGERVELIVASHDEVGRARETLSYVAVGDITVDEPTRTLTAPVAEGHKAL
                     TDVLRGLDANQITILDIGLRRPTLDDVFLTLTGQGTEEVDRTEKQEVA"
     gene            1133..2098
                     /locus_tag="VFZ80_02535"
     CDS             1133..2098
                     /locus_tag="VFZ80_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX6286335.1"
                     /translation="MSGTTSRALRLLSLLQSRRVWTGADLMERLAVSERTLRRDIDRL
                     RDLGYEVDSVTGPAGGYQLQAGTDIPPLLLDDEEATAIAVGLMTAAGGSIEGLEETSL
                     RALTKLEQVMPPRVRRQVATLQAAVVPLVRAWVTVDAGVLTNVAQACRDHERIRFEYQ
                     TRGGERAERHVEPHQLVSIHQRWYLLAFDRDREDWRTFRLDRMWSPWGTRVRFEPRPI
                     PGGSAADYVEQSLRSMPMRYRAEATVHAPAGDVAERLKHGDAEVEAIDEGRCRVRIKG
                     DALEWLAFTLIWQGFEFEVHEPAELIQFLTDLSHRLARAAHPLIT"
     gene            2124..2552
                     /locus_tag="VFZ80_02540"
     CDS             2124..2552
                     /locus_tag="VFZ80_02540"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6286336.1"
                     /translation="MELTRTQEEFIEMNPSAAMITIGEDGFAKAVRVGIAVIDGEVWS
                     SGTAERVRTDRLREDPRCTLFVFGPGYEALTLEGHISLIEGSEAVDESVQLFRQMQDR
                     PQGSLSWYGEELDEDRFRQTMVDQQRLIYVLDVMRAYGVE"
     gene            2631..3176
                     /locus_tag="VFZ80_02545"
     CDS             2631..3176
                     /locus_tag="VFZ80_02545"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX6286337.1"
                     /translation="MRKTIVAALVAALTLALAIPALGRSSKAAPVKGSDSIATIAGEA
                     GFTELVGALMYVDAELGTNLVGLFSEGTDQYTVFAPTNAAFQGLYSDLGVGGIEDLDA
                     ELVLDVLLYHVTEGRRAANSVVPESGERTITSLMGAKFTVDSGLDIDDLADRDADIVS
                     ANISASNGIIHVIDDVILPLS"
     gene            complement(3267..4418)
                     /locus_tag="VFZ80_02550"
     CDS             complement(3267..4418)
                     /locus_tag="VFZ80_02550"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6286338.1"
                     /translation="MISALFFTWFAAASMWTVNALRRPVLPGAALPPLWLPGMLISEL
                     APAYFVLRILVALGFLSVGAAELGIGKAGLVLFALSEVGLLWIMGRTLRGAGATGHSP
                     SPLTIFRLWERLPVEVRHIEEVTYWDSLTLDVYAKPQLADAPSLVYVHPGSWMRGRPG
                     RQARAMFHRLAARGWVVLDIRYPLSPDATFPEHLIGVKRALAWARDGGSSYGIDPARV
                     AVSGGSSGAHLAALAALTSDDKALQPGFEDEDTSVAACVPFYGIYDLLVRNPTRYDWP
                     FIAKVVMKAGAAEAPDLYSLGSPIDQVHHGAPPFLVIHGEFDSVVLPAESEHFVAALR
                     DHGVDTGYFEVPGAQHGFDAIASLRTRAVANMCVEWLEANVTPRPRGEC"
     gene            4487..5449
                     /locus_tag="VFZ80_02555"
     CDS             4487..5449
                     /locus_tag="VFZ80_02555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6286339.1"
                     /translation="MPSNALTRRAATIAVVFTLLITVTLLAPVLVVVGALLDLARQVS
                     GVKTWVTLRSLGFLWVYLVGEVWAVVALLGTAPLPEKAKLDLTFRLQQRWASWNLKAL
                     VALFGLEVVVEGAEAAFPGPVVVLSRHASMVDTLLPAAIISRRDGIRLRYVLKKELLI
                     DPALDIAGNRLPNVFIDRKSGEASERSAIRALAEDLGPRDGVLIYPEGTRYSEAKLRR
                     LQGKASNPAAAGFRRVLPPRPGGTLAILETANADVIVFAHHGLEGLATAREIWAGGLV
                     GSRISVRMWRIEKDQIPSGRSERVEWLNALWAEIDDWVVSVGAS"
     gene            5446..>5787
                     /locus_tag="VFZ80_02560"
     CDS             5446..>5787
                     /locus_tag="VFZ80_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX6286340.1"
                     /translation="MTSVVWFRRDARLDDNPALTAASAEGDVCALFVIDPYLYDRVSS
                     RRRDLLIAGLHELDRRLGERGGRLRISRGEPARVVPAVAREVGADAVHVNSEVTPHGV
                     SRDRDVAEVVSL"
BASE COUNT         1022 a         1804 c         1893 g         1068 t
ORIGIN      
        1 cactcgcttg atcttgatca ggtttcgctg ggcaacggca aggccgtccg cagccatcca
       61 agccaggttg gtcatcacgc cacctcctgc ttctcggttc ggtcgacctc ttcggtgccc
      121 tgaccggtga gggtgaggaa cacgtcatcg agggtcggtc gtctcagacc gatgtcgagg
      181 atcgtgatct ggttggcatc gaggccccgg agcacgtccg tcagggcctt gtgcccttcc
      241 gccaccggag ctgtgagggt ccgggtgggc tcgtcgactg tgatgtcacc gacagccacg
      301 tagctgagag tctcccgggc gcggcccacc tcatcgtggg aggcgacgat cagctcgaca
      361 cgctctccac cgatgtcgct cttgagctga tcggccgttc cccgggcgat ggcgcggccg
      421 tggtcgatga cgacgatctc gtcggcgagg cggtcggcct cctccaggta ctgcgtggtg
      481 agcaagagcg tggtgcccgc cccgaccatc tccctgatca catcccacat gtcggcccga
      541 ccacgggggt cgagaccggt agtgggctcg tcgaggaaga gaaccgtcgg gttggcaacc
      601 agggctccgg cgaggtcgag acgacgtctc atgcctcctg agtaggtctt gacgggccgg
      661 tcaccggctt cgatcagatc gaagcgctcc aacagttccc cggctctcat ccgcgccttg
      721 tctcggccga ggcgatacaa cttgccgaac agatagaggt tctcgaatcc ggtcaggtac
      781 tcgtcgacgg cggcgtactg acccgacagc ccgatggtgg agcgcaccgc gttcgggttt
      841 gccacgacgt cgatcccggc gacggtcgcc gacccttcat cagggacgag cagagtggtc
      901 aggatgcgga ccgcagtcgt cttcccggca ccgttgggac cgagcagacc gagcacagtt
      961 ccctgaggga cgctgaggtc gaggccgtcg agagccctca gctccccata gttcttcacg
     1021 agcccttcgg cccgtatcac ttcgttcatt tggtgtcctt tcgaccctgc cggggcgacc
     1081 atatttgcga ttcatgtcag aaattgaccg gaatctatac catcttccag acatgtccgg
     1141 gacgacgtca cgagccttgc gtcttctctc acttctccag tcacgacgtg tctggaccgg
     1201 tgcggatttg atggaacgac tcgccgtcag tgagcggacg cttcgtcggg acatagaccg
     1261 gctccgtgat ctcggctacg aggtcgattc ggtcaccggt cccgccggag gttatcaact
     1321 ccaggcgggc acagatattc cgcctcttct cttggatgac gaggaggcca cggccatcgc
     1381 cgtgggtctc atgaccgccg ccgggggaag catcgaaggg ctggaggaga cctccctgcg
     1441 cgccctcacc aagcttgaac aggtgatgcc tccccgcgtc cgcagacagg tggcgaccct
     1501 gcaggcggcc gtcgtgcccc tggtcagggc atgggtgact gtcgacgccg gtgttctgac
     1561 caatgttgct caggcgtgtc gagaccacga gaggattcgc ttcgagtatc agactcgcgg
     1621 gggcgagcga gccgagcgcc atgtcgagcc gcaccaactg gtctccatcc accagcggtg
     1681 gtatctgctc gcattcgacc gcgatcgtga ggactggcgc accttccggc tcgaccgaat
     1741 gtggtcaccg tgggggacgc gggtcaggtt cgagccgaga ccgataccgg gcggcagcgc
     1801 cgccgactac gtcgaacagt ctctccgctc catgcccatg cgatatcgcg cagaggccac
     1861 cgtgcacgcc ccggccggcg atgtggccga acgtctcaaa catggggatg ccgaggtgga
     1921 ggccatagac gagggtcgct gccgggtgcg tatcaaaggc gacgccttgg agtggctggc
     1981 gttcacgctg atctggcagg gattcgagtt cgaggttcat gagcccgccg agctcatcca
     2041 gttcctgacg gatctatcgc acagactggc ccgcgccgcc cacccgttga ttacctgaac
     2101 cgggtcgcgg gtaattgggc tccatggagc tcacacgaac ccaagaggag ttcatcgaaa
     2161 tgaatccgtc ggccgccatg atcacgatcg gcgaagacgg cttcgccaaa gcggtgaggg
     2221 tggggattgc cgtgatcgac ggtgaggtct ggagtagcgg gaccgccgag cgggttcgca
     2281 ccgaccggtt gcgggaggac ccgcgctgca cgctgttcgt cttcggcccc ggctacgagg
     2341 ctttgacatt ggagggacac atctcattga tcgagggaag tgaggccgtc gacgagtcgg
     2401 ttcagctctt tcgtcagatg caggatcgtc ctcaagggtc actctcctgg tacggcgagg
     2461 agttggacga ggaccgtttc aggcagacga tggtggacca gcagcgattg atctacgtcc
     2521 tcgacgtgat gcgggcttac ggggtggagt aatcggcaca tttctctcat ccggtctcga
     2581 cgtcggttcc gaacactgtg cgtagtcgag aagcacagga ggcttcggaa atgagaaaga
     2641 caatcgttgc cgctctcgta gcggctctga ccctcgcttt ggcgatcccg gctcttggcc
     2701 ggagtagcaa agctgcgccc gtgaagggct cggactccat cgccaccatc gcgggcgaag
     2761 ccgggttcac agaactggtc ggggctctga tgtacgtcga cgccgagctg ggaacaaacc
     2821 tcgtcggctt gttcagtgag ggcaccgacc agtacaccgt cttcgccccg acgaacgctg
     2881 cattccaggg tctttacagc gacctcgggg tcggcggtat cgaggacctc gatgccgaac
     2941 tcgtcctcga cgttctgctc tatcacgtga ccgagggtcg tcgcgctgcc aacagcgtgg
     3001 tgcccgagtc gggtgagcgg accatcacgt cgctgatggg agcgaagttc actgtcgaca
     3061 gcgggctcga catcgacgat ctcgccgatc gcgatgctga catcgtgtcg gccaacatct
     3121 cggcgagcaa cgggatcatc cacgtcatag acgacgtgat cctgccgctg agctgacctg
     3181 gccctcctcc agctagggtg aagaggcccg ccgattcggc gggcctcttc gtgtgtgcca
     3241 aaacaccggc agggttggcg ccgcctttaa cactcccccc tcggtcgtgg cgtgacgttc
     3301 gcctcgagcc attcgacaca catgttggcc acggcacggg tgcgaagact ggcgatggcg
     3361 tcgaaaccgt gctgtgcacc gggcacttcg aagtagccgg tgtcgacacc gtggtcacgg
     3421 agagcggcga cgaagtgctc ggactcggcg gggagcacga cggaatcgaa ctcaccatga
     3481 atgaccagga aaggcggcgc accgtgatgg acctggtcga tcggcgatcc cagggaatac
     3541 aggtcgggcg cctcggccgc cccggccttc atgacgacct tggctatgaa cggccagtca
     3601 taacgggtgg ggttccgcac caggaggtcg tagatcccgt agaagggaac acatgcggcc
     3661 accgatgtgt cttcatcctc gaaacccggc tggagagcct tgtcatccga ggtcagggcg
     3721 gccagcgcgg cgagatgtgc ccccgaggac cctcctgaga ccgcgacgcg ggcagggtcg
     3781 atcccgtagc tggatccacc gtcccgcgcc caggccagtg ctctcttcac accgatgaga
     3841 tgctcgggaa aggtggcatc aggagagagg gggtacctga tgtcgagcac gacccatcct
     3901 ctcgcggcca ggcggtgaaa cattgcccgc gcctgtcggc ccggccggcc ccgcatccac
     3961 gacccggggt ggacatagac gagagacggc gcgtcggcga gttgcggctt ggcgtagaca
     4021 tcgagcgtca gtgagtccca gtaggtgacc tcttcgatgt gccgaacttc gacaggcaga
     4081 cgctcccaca gtctgaagat ggtgagcgga gacggcgagt ggccggttgc gcccgcaccg
     4141 cgaagcgttc tccccatgat ccaaagcaga ccgacctcag acagtgcgaa cagcaccagg
     4201 ccggccttgc cgattcccag ttcggccgca cccacggaga ggaaccccaa ggcgacgagg
     4261 atccggagca cgaagtaggc cggcgcgagc tcggagatca gcatgcccgg gagccacaat
     4321 ggcggtagag ccgcgccggg caggaccggt ctacgcagcg cattgacggt ccacatggat
     4381 gccgcggcga accaggtgaa gaagagggcc gagatcaatc ggtcacccgt gagggtggga
     4441 ccatccgcgg aagcatcggc tccgaactta gtgtgacttt gctcccatgc cttccaatgc
     4501 actgacccga agagcggcca cgatcgcggt ggtgttcacc ctcctgatca cggtgacgct
     4561 cctcgccccg gttctcgtgg tggtgggagc cttgctggat ttggcccgcc aggtctccgg
     4621 cgtcaagaca tgggtgacgc tccgatccct gggtttcctg tgggtctacc tggttgggga
     4681 agtctgggca gtagtcgcgc tcctgggcac ggctcccctc cccgagaagg ccaagctcga
     4741 cctcaccttc cgcctgcaac agagatgggc gagctggaat ctgaaggcgc tcgtggcact
     4801 cttcggtctc gaggtcgtcg ttgagggtgc cgaggcagcc ttccccggcc ctgtggtcgt
     4861 gctgtcacgc catgcctcga tggtcgacac gctgcttcct gcggcaatca tctcgagacg
     4921 cgacggcatc agattgcgct acgtcctgaa gaaggagctt ctgatcgacc ccgccctcga
     4981 catcgctggc aaccggctgc ccaacgtctt catcgaccgc aaatccggtg aggcgtccga
     5041 gcgctctgcg atccgggccc tggccgaaga cctggggcct cgtgacggag tgctcatcta
     5101 cccggagggc accagatact cggaggcgaa gttgagacgg ctccagggaa aggcctccaa
     5161 cccggctgcg gccggctttc ggagggtgct gccaccgcgg ccgggcggga cactcgccat
     5221 tctggagacg gcgaacgccg acgtgatcgt cttcgctcat catggtctcg aaggtcttgc
     5281 caccgctcgt gagatatggg ccggtgggct cgtgggatcg cggatctcgg tccgaatgtg
     5341 gcgcatcgag aaggatcaga ttccctctgg tcgttccgag agggtcgagt ggctcaatgc
     5401 gctctgggcc gagatcgacg attgggttgt ctcggtgggt gcgtcatgac atctgttgtc
     5461 tggttccgtc gtgacgcccg tctcgacgac aatccggcgc tcaccgccgc cagcgccgag
     5521 ggcgatgttt gtgccctgtt cgtgatcgac ccctacctct acgacagggt ctcgagccgt
     5581 cgccgtgacc tgttgatagc gggtctccac gaactggatc gccgtctcgg tgagcggggg
     5641 ggaaggctcc gcatttcgag aggcgagccg gcacgcgtcg tgcccgcagt tgcccgcgag
     5701 gtgggagccg atgccgttca cgtcaattca gaggtcacgc cgcacggtgt atcccgtgac
     5761 agagacgtgg ccgaagtggt ctccctg
//
LOCUS       DASUTA010000126         2541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_39253, whole genome shotgun sequence.
ACCESSION   DASUTA010000126 DASUTA010000000
VERSION     DASUTA010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39253"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /gene="leuD"
                     /locus_tag="VFZ80_02565"
     CDS             complement(<1..404)
                     /gene="leuD"
                     /locus_tag="VFZ80_02565"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012574285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX6286341.1"
                     /translation="MKPVSVIKGRMVTLPQADVDTDQIIPKQFLTRTERTGYGDFLFY
                     DWARTSDGTPSSDFFTNDPDRQGAKVLVAGRNFGCGSSREHAVWAIQDWGFDAVIAPS
                     FGDIFSNNCVNVGLLAVVLPPDVVDRIHDLAQ"
     gene            complement(401..1798)
                     /gene="leuC"
                     /locus_tag="VFZ80_02570"
     CDS             complement(401..1798)
                     /gene="leuC"
                     /locus_tag="VFZ80_02570"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX6286342.1"
                     /translation="MSARTMFEKIWDDHVVTGDDPALLYVDLHLVHEVTSPQAFAGLD
                     FNGRTVRRPDLTVATVDHAIPTTARSLGIRDPQSKKQVDTLKANTEKHGITFFGLDDP
                     RQGIVHVVGPENGYTQPGKVIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLWQK
                     RPRTMAVEFDGDLPDGVTAKDMILGMISQLGVGGGVGHVIEYRGEAIRHLSMEERMTV
                     CNMSIEGGARAGMIAPDETTFEYLEGRPAAPKGSDWDEAVAYWAGLVTDEDSVFDTTR
                     HINAASLVPHVSWGINPGQTAPITASVPDPDSFTDPMEKDSVVRALQYMALKPGTPFT
                     DIAIKHVFVGSCTNGRIEDLRNAAKVVAGKRVAKGVNAVVVPGSMQVKAEAEREGLDE
                     IFTSAGFSWREAGCSSCLAMNDDVIPPGERCASTSNRNFEGRQGRGARTHLLSPAMAA
                     AAALAGHLVDVREWS"
     gene            complement(1795..>2541)
                     /locus_tag="VFZ80_02575"
     CDS             complement(1795..>2541)
                     /locus_tag="VFZ80_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX6286343.1"
                     /translation="PGVRSPIADVEPEDVDFVVVRENSEGLYVGKGSFTAKGSPFEVA
                     VQESVNTRHGVERCVRYAFDLARSRPARHLTLCGKTNVLKYSWDLWARVFNEVATEYE
                     DVETAYAHVDAACLWMVEDPARFDVIVTDNMFGDIITDLAAAIQGGLGIASGANLNPE
                     GVSMFEPIGGTAPGFEGTGKINPLAAIGAAAMMLRQLDEADAADAIEDAVARTAGSLP
                     SLRAGEMGKTTEEVGAQVAELAKQPAQVPA"
BASE COUNT          448 a          809 c          804 g          480 t
ORIGIN      
        1 ttctgggcca ggtcatggat gcggtcgacc acgtcgggag gcagcaccac ggccagcaga
       61 ccaacgttca cgcaattgtt ggagaagatg tcaccgaacg aaggggcgat cacggcgtcg
      121 aaaccccagt cctgaattgc ccagaccgcg tgctctcgcg acgacccgca tccgaagtta
      181 cgtcccgcga cgaggacctt ggcgccctgt cggtcagggt cgttggtgaa gaagtccgag
      241 ctcggtgtcc catccgaagt cctcgcccag tcgtagaaca ggaagtcgcc gtaccccgtg
      301 cgctccgttc gtgtcaggaa ctgcttgggg atgatctggt cggtgtcgac gtcggcttgg
      361 ggcagggtga ccattcgccc cttgatcacc gagacgggtt tcacgaccac tccctcacgt
      421 ccacgaggtg gcctgccaaa gccgcagccg ccgccatcgc cgggctcaac aggtgagtgc
      481 gagctccacg gccctggcgc ccttcgaagt tccggttcga agtcgacgca cagcgttccc
      541 cgggcgggat cacgtcgtcg ttcatggcca gacagctgga gcacccagcc tcgcgccagg
      601 agaagccggc agaggtgaag atctcatcga ggccctcgcg ctcggcctcc gccttcacct
      661 gcatcgaccc ggggacgacc acggcgttga cgcctttggc cacccgcttg ccggctacca
      721 ccttcgccgc attgcggaga tcctcgatcc ggccgttggt gcacgagccc acgaagacat
      781 gcttgatggc gatatccgtg aacggggttc cgggtttgag ggccatgtat tggagtgcgc
      841 gcaccaccga gtctttctcc atcgggtcgg tgaacgagtc cgggtcggga acagacgctg
      901 tgatcggcgc ggtctggcca ggattgatac cccagctcac gtggggcacg agcgaggcgg
      961 cgttgatgtg acgcgtggtg tcgaaaacag aatcctcgtc ggtcaccaga cccgcccagt
     1021 aggccacggc ttcgtcccaa tcagagcctt ttggagcggc aggacgacct tccaggtact
     1081 cgaatgtcgt ctcgtcggga gcgatcatgc ccgcccgggc gccgccttcg atcgacatgt
     1141 tgcacacggt catccgctcc tccatcgaga ggtggcggat cgcttcgcct ctgtactcga
     1201 tcacgtggcc gacaccgcca cccacgccga gctgagagat catgccaagg atcatgtctt
     1261 tggctgtgac tccgtcgggc agatccccat cgaactcgac agccattgtt cgcggcctct
     1321 tctgccacag ggtctgggtt gccagcacat gttcgacctc cgatgtcccg ataccaaagg
     1381 cgagcgcacc gaatgcaccg tgagtcgagg tgtggctgtc accgcacacg atcacctttc
     1441 caggctgggt gtacccgttc tcgggaccga ccacgtggac gattccctga cggggatcgt
     1501 cgaggccgaa gaaggtgatg ccgtgctttt cggtgttggc tttgagagtg tcgacctgct
     1561 tcttcgactg cggatcccgg atgcccagtg agcgcgccgt ggtcgggatg gcgtgatcaa
     1621 cggttgccac ggtgagatcg ggtcggcgca ccgtgcgccc gttgaagtcg agaccggcga
     1681 acgcctgcgg tgatgtgacc tcgtggacca gatggaggtc gacgtataga agtgcggggt
     1741 cgtcgccggt gacgacgtga tcgtcccaga tcttctcgaa catggtgcgg gcgctcatgc
     1801 cggaacctgg gcaggctgct tggccaactc agcgacttga gcaccgactt cttcggtcgt
     1861 cttacccatc tcacccgccc ggagtgaagg gagggagccg gcagtcctcg ccacggcgtc
     1921 ctcgattgcg tcggcggcat cggcctcatc cagttgacgc agcatcatcg ccgccgcgcc
     1981 gatggcggcc agtgggttga tcttgccggt tccctcgaag ccgggggcgg tgcccccgat
     2041 cggttcgaac atcgaaacac cctcaggatt gaggttggct ccagaggcta ttcccaggcc
     2101 gccctgtatc gcagcggcga ggtcggtgat gatgtctccg aacatgttgt ccgtgactat
     2161 cacgtcgaag cgggctggat cctcgaccat ccacagacac gctgcgtcga cgtgggcgta
     2221 cgccgtctcg acgtcctcgt actcggtggc cacctcgttg aacacccgtg cccacagatc
     2281 ccacgagtac ttgaggacat tcgtcttccc gcagagcgtc aggtggcggg cgggtcggct
     2341 gcgggcgagg tcgaaggcgt aacgaacgca ccgctcgaca ccgtgacggg tgttgaccga
     2401 ctcctggaca gccacctcga acggtgagcc tttggccgtg aacgagccct tacccacgta
     2461 aagaccctca ctgttctcgc gaacaacgac gaagtcgaca tcttcaggtt cgacatcggc
     2521 gatgggggag cggacccctg g
//
LOCUS       DASUTA010000127         3102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_815669, whole genome shotgun sequence.
ACCESSION   DASUTA010000127 DASUTA010000000
VERSION     DASUTA010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815669"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFZ80_02580"
     CDS             <1..273
                     /locus_tag="VFZ80_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286344.1"
                     /translation="LRFGDNPPEPMSALILERLKRRRIPRIFFDALTHRDLDAIADFE
                     VWNDLKTFELVADPPVAAQADGESLGMIDGGELTWEGDSLRVLAGN"
     gene            complement(270..1139)
                     /locus_tag="VFZ80_02585"
     CDS             complement(270..1139)
                     /locus_tag="VFZ80_02585"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX6286345.1"
                     /translation="MEFLAQELDELTTDNPLISSLMIVGVFALLYWGATLSGARYLDR
                     MHKKGHEAGARAETLWLVMRRVVALVIVFLAVLFIFLTWGWSIAPFLGVGTVLAAAIG
                     FGAQDVVKDFLSGFFILMEDQFHVGDVITIAGINGTVTTGTAEDIQLRVTVLRDVEGN
                     VHYVPNGQIAVISNFTSKYAQPVLDIGVAYETDIDHALDVMRDELGKLAADPAYSDLI
                     NGDPEILGVNELGDSSVILRGRLTTVADERWSIRREALRRIKKRFDAEGISIPFPQVT
                     INQKIVASDDEQS"
     gene            complement(1152..2324)
                     /locus_tag="VFZ80_02590"
     CDS             complement(1152..2324)
                     /locus_tag="VFZ80_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX6286346.1"
                     /translation="METAELAVASSSGVVSKETLEPLVNLLKTIRLRMSFPEDVLVVA
                     LAGGTGSGKSSLFNALAGDELVDVGGVRPTTSEPAASVPRSAGSRFEGFLDGIGINRR
                     HDHDDLAFCLIDLPDNDSVEVAHHHRVDELLPVVDVVVWVTDPEKYRDSRLHHDYLQP
                     LSENAERFVIVLNQIDRLAPDDLEAILADLGDALESDGISRAVLLPMAVTPPAGPSIG
                     IEGLVSRLESMRAGRDVVEAKLIADLAATARSLAAGAGEPLDFDVRASSALSDATASL
                     EEGDRASAISRLTAFLDEVAAEVGGPSGREIARMSADVPAHVGRIDSEMRSGESRRRW
                     FQRRRGPEFDSDAARSKLNEAMIRPARSVVARRAVALASIADLAVEVESLGRDPSR"
     gene            complement(2341..>3102)
                     /locus_tag="VFZ80_02595"
     CDS             complement(2341..>3102)
                     /locus_tag="VFZ80_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286347.1"
                     /translation="LRRQLVSLADDRASSSERIFDRVLRATISQAEAFRVEVSETIAK
                     DDASREEVAESLVARVGDLDLSGVGDGLVDRPNGQTTREVRRWRRSSRRGALTKDEVE
                     TVVGSIEARLVRDLRIWMSELPDSAGFEIEPRRFLIGIAPVARMALEGWIDFVRRIAL
                     EEAARSVGLAQAVLVATATRPSDNLAADILFGRRADDVVSRARRELVGRLDVIYEQAA
                     VQANELSRLESGVVDLSDLQAALASVRLRLALVDA"
BASE COUNT          580 a         1025 c          966 g          531 t
ORIGIN      
        1 ctccggttcg gagacaaccc gcccgagccc atgtccgccc tgatcctgga gaggctcaag
       61 cggcgtcgca tccccaggat cttcttcgac gccctcaccc atcgcgacct cgacgccatt
      121 gccgacttcg aggtgtggaa cgatctgaag accttcgaac tggtagccga tcctcccgta
      181 gcggcccagg ccgacgggga gagtctggga atgatcgacg gtggcgagct gacctgggag
      241 ggcgacagcc tcagggtcct ggccgggaac tagctctgtt cgtcgtctga ggcgacgatc
      301 ttctggttga tcgtcacttg cgggaacggt atggatatcc cctcggcatc gaaacgcttc
      361 ttgatgcgtc gcagcgcctc gcggcggatc gaccagcgct cgtcggcgac ggtggtcagg
      421 cgtcctcgca gtatcactga tgagtcaccc agttcgttga cacccaggat ctccggatcc
      481 ccattgatga gatcgctgta cgccgggtcg gctgccaact tcccgagctc gtcacgcatg
      541 acgtcgaggg cgtggtcgat gtcggtctcg tacgccacac cgatgtcgag cacgggctgg
      601 gcatatttcg aggtgaagtt ggagatcacg gcgatctgcc cgttgggcac gtagtggacg
      661 ttcccctcta catcgcggag cacggtcacg cgcagctgta tgtcctcggc cgtgccggtg
      721 gtaacggtcc cgttgatgcc ggcaatggtg atcacgtcac cgacatggaa ctggtcctcc
      781 atcaggatga agaaccctga taggaagtcc ttgacgacgt cctgggcacc gaagccgatg
      841 gcagcggcga gcacagtgcc cacccccagg aacggggcga tgctccaacc ccaggtcagg
      901 aagatgaaga ggacggccag gaacacgatc acgagggcaa ccacccggcg catgaccaac
      961 caaagggtct ccgctcgcgc ccccgcctcg tgacccttct tgtgcatccg gtctagatac
     1021 cgggcgccgg agagcgtcgc cccccagtag agcaatgcga agacaccgac gatcatcaga
     1081 ctggagatga gcgggttgtc cgtggtcagc tcgtcgagct cttgggccag gaattccatc
     1141 gaccagccag gttaacgaga cgggtctcta cccagactct ccacttcgac ggcgaggtct
     1201 gcgatcgagg ccagggccac ggcccgtctc gccacgacag atcgggcagg gcggatcatc
     1261 gcctcgttga gtttcgacct ggccgcgtca ctgtcgaact ccggtcctct gcgtcgctga
     1321 aaccatctgc gcctgctctc gcccgaccgc atctcggagt cgatccggcc cacatgcgcc
     1381 ggcacatccg ccgacatgcg agcgatctcc cgcccggagg gccctcccac ctcggcggcg
     1441 acctcgtcga ggaaggcagt cagcctcgag atcgcgcttg ctcgatctcc ctcttcgaga
     1501 ctcgcggtgg cgtcggacag tgcggaggac gccctcacgt cgaagtccag cggctccccg
     1561 gcacctgcgg ccagcgaccg ggccgtggcg gccaggtcgg cgatgagttt cgcctccacc
     1621 acgtctcgac ccgcgcgcat ggactcgaga cgactcacca gaccctcgat gccgatgctc
     1681 ggccccgccg gcggggtgac cgccatgggc agcagcacag ctctcgagat gccatccgac
     1741 tcgagggcat caccgaggtc ggcgagaatt gcctccagat catcaggagc gagtcgatcg
     1801 atctgattga ggacgatcac gaatcgctct gcattctccg agagcggctg gaggtagtcg
     1861 tggtgcaggc gagagtcccg gtacttctcg ggatcggtca cccacacgac gacgtcgacc
     1921 acggggagca gctcgtcgac acggtggtga tgggcgacct cgacgctgtc gttgtccgga
     1981 aggtcgatca agcagaaggc caggtcatcg tgatcgtgcc gacggttgat gccgatgccg
     2041 tcgagaaagc cttcgaaacg ggacccggcg ctccgtggaa cagatgccgc aggctcgctc
     2101 gtggtaggtc tgacaccacc gacgtcgacg agctcgtcac cggcgagggc attgaagagc
     2161 gatgacttgc ccgatccggt gcctccggcg agggccacca ccaggacatc ctcaggaaag
     2221 gacatacgca gcctgatcgt cttcaggagg ttgacgaggg gctcgagtgt ctccttcgac
     2281 accacccccg atgacgaggc aacggcgagc tccgctgtct ccaccaggtg aatgaggctc
     2341 tcacgcatcg acgagagcga gacgcaacct cacggaggcg agggctgcct gcaagtccga
     2401 gagatcgacg acaccactct ccagccgtga cagctcgttt gcctggacag ctgcttgctc
     2461 gtatatcacg tcgagacggc ccactagctc acgtcttgcc cggctgacga cgtcgtcggc
     2521 tcgtcgaccg aacaagatgt cggctgcgag attgtccgag gggcgggtag ccgtcgcaac
     2581 caacaccgcc tgtgccagcc cgacgctccg agccgcctct tccagggcga ttcgtctcac
     2641 gaagtcgatc cagccctcca gcgccatcct ggcgacaggg gcgatgccga tcagaaatcg
     2701 acgcggctca atttcgaaac ccgccgaatc gggcaactcc gacatccaga tgcggaggtc
     2761 gcggacaagg cgagcctcga tcgagccgac aactgtctcg acctcgtcct tggtgagggc
     2821 gccgcgacgc gaagaccggc gccatctcct gacttcgcgt gtggtctgcc cgttcggtcg
     2881 atcgacgagc ccatccccca caccagacag atcgagatcg ccgacgcgag caacgagcga
     2941 ttccgccacc tcctcccgtg aggcatcgtc tttggcgatg gtttccgaca cttcgacccg
     3001 gaaggcctct gcctggctga tcgtggcgcg gagcactcga tcgaagatcc gctcgctgct
     3061 cgaggcacgg tcgtcggcga gtgacacgag ctggcggcga ag
//
LOCUS       DASUTA010000128         3384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_557815, whole genome shotgun sequence.
ACCESSION   DASUTA010000128 DASUTA010000000
VERSION     DASUTA010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557815"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..425
                     /gene="rplP"
                     /locus_tag="VFZ80_02600"
     CDS             12..425
                     /gene="rplP"
                     /locus_tag="VFZ80_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX6286348.1"
                     /translation="MLMPKRTKHRKTHRGRMSGVAKGGTTVNFGEYGLQALEPGWITA
                     RQIESARVAITRAVKRGGKVWITIFPDKPVTQKPAETRMGSGKGNPEFWVSVVKPGRV
                     MFELAGVDEALAREAMRKASHKLPIKTRVVTREDA"
     gene            422..679
                     /gene="rpmC"
                     /locus_tag="VFZ80_02605"
     CDS             422..679
                     /gene="rpmC"
                     /locus_tag="VFZ80_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX6286349.1"
                     /translation="MSLSDLREMTEAELLDALVEAKEERFNLRFQIATNQLDNTARLK
                     TVKKEIAQILTILRERAASAEAEAVAVANATAPGSDVEETA"
     gene            679..945
                     /gene="rpsQ"
                     /locus_tag="VFZ80_02610"
     CDS             679..945
                     /gene="rpsQ"
                     /locus_tag="VFZ80_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015450963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX6286350.1"
                     /translation="MAEDTTRTSRKVRVGVVVSDARDKTVTVEVASSSAHPRYDKVVR
                     TSSKFHAHDEANDAHVGDTVRIMETRPLSKQKRWRVVEIVERAR"
     gene            942..1310
                     /gene="rplN"
                     /locus_tag="VFZ80_02615"
     CDS             942..1310
                     /gene="rplN"
                     /locus_tag="VFZ80_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003940951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX6286351.1"
                     /translation="MIQQESRLKVADNTGAREVLCIRVLGGSSRRYAGVGDVIVGTVK
                     EATPGGSVKKGEVVRAVVVRTKKESRRDDGTYIRFDDNACVLIDPNRQPRGTRIFGPV
                     ARELRDERFMRIVSLAPEVL"
     gene            1495..1800
                     /gene="rplX"
                     /locus_tag="VFZ80_02620"
     CDS             1495..1800
                     /gene="rplX"
                     /locus_tag="VFZ80_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX6286352.1"
                     /translation="MKLKKGDTVMVIAGKDTGKESKVTRVLPRKGKVQVEGVNTAKRH
                     TKPTGQTMQGGIIDKDMPIDASNVMIVCEKCGPTRIGHRIDDKGNKHRTCVKCGGDL"
     gene            1797..2384
                     /gene="rplE"
                     /locus_tag="VFZ80_02625"
     CDS             1797..2384
                     /gene="rplE"
                     /locus_tag="VFZ80_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX6286353.1"
                     /translation="MSAKTGTKTSTKPRLKARYDDEIHAQLKEQLGLVNPMEVPTLEK
                     IVLNMGLAEAKDNKKVIDEAIDELGLIAGQKPRVNKARKSIANFKLREGMPIGVSVTL
                     RGYRMWEFFDRLLSVAIPRVRDFRGLNPRAFDGRGNYSVGASEQLIFPEIDFDSVSET
                     RGMNITIVTTADNDEAGKALLDAFGFPFRRVETTV"
     gene            2401..2586
                     /locus_tag="VFZ80_02630"
     CDS             2401..2586
                     /locus_tag="VFZ80_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX6286354.1"
                     /translation="MAKKSMIAKANKKAKFKVREYNRCQRCGRPRAFIRDFGMCRICV
                     RELASRGELPGVRKASW"
     gene            2589..2984
                     /gene="rpsH"
                     /locus_tag="VFZ80_02635"
     CDS             2589..2984
                     /gene="rpsH"
                     /locus_tag="VFZ80_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX6286355.1"
                     /translation="MVTDPISDMLTRIRNAVAVGHERVAMPSSKLKVNIAQILVDEGF
                     IDRYEVSENGHRSDLELVLRYAERRRPVIEGLKRVSRPGHRVYRGAAELPRVQGGLGV
                     AVVSTSQGLMPDREARKRRLGGEIICEVW"
     gene            2984..>3384
                     /gene="rplF"
                     /locus_tag="VFZ80_02640"
     CDS             2984..>3384
                     /gene="rplF"
                     /locus_tag="VFZ80_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003940970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX6286356.1"
                     /translation="MSRVGNKPVDLPDGVDVKIDGSVVTVKGSKGELARTFHERIGFD
                     LDDGVVSVTRPDDERESRALHGLSRALLANMVTGVSDGFRKELEIHGVGYRASMKGTD
                     IELLVGFSHPVVVEAPEGVTFEVPEQTRIVVS"
BASE COUNT          698 a         1034 c         1086 g          566 t
ORIGIN      
        1 gagtagcggt catgctcatg ccaaagcgca ccaagcatcg aaagacgcat cgaggccgca
       61 tgtcaggtgt ggccaagggc gggaccaccg tgaacttcgg agagtacggg ctacaggccc
      121 tcgaaccggg gtggatcacc gcccgtcaga tcgaatcggc ccgtgttgcc ataacccgcg
      181 ccgtcaagcg cggcgggaag gtctggatca ccatcttccc ggacaagccc gtgacccaga
      241 agccggccga gacccgcatg ggctcgggaa aggggaaccc tgagttctgg gtgtccgtcg
      301 tgaagccggg ccgtgtcatg ttcgagctgg cgggtgtcga tgaggccctc gctcgtgaag
      361 cgatgcgcaa ggcctcacac aagctcccga tcaagacccg ggtcgtgacc agggaggacg
      421 catgagcctc agcgacctcc gggagatgac cgaagccgag cttctcgacg ccctggtcga
      481 ggccaaggag gagcgattca accttcgctt ccagatcgcg accaatcagc tcgacaacac
      541 ggcccgcctg aagacagtga agaaagagat tgcccagatc ctcacgatcc tccgtgagcg
      601 tgcggcttcg gctgaagccg aagccgttgc agttgcaaac gcaactgccc cgggctccga
      661 tgttgaggag accgcctgat ggctgaagac acaaccagga cgagcagaaa ggttcgcgtg
      721 ggcgtcgtgg tttctgacgc acgcgacaag acggtgaccg tggaggtcgc cagctcgtcc
      781 gctcatcccc gctacgacaa ggtagttcgc acgtcgtcca agttccatgc gcacgacgag
      841 gcgaacgatg cccacgtcgg cgacacggtt cgaatcatgg agacgcgccc cctctcgaag
      901 cagaagcgct ggcgtgtcgt cgagatcgtg gagcgtgctc gatgatccaa caggaatccc
      961 gactcaaggt ggccgacaac accggtgccc gcgaggtgct ctgcatccgc gtgctcggtg
     1021 ggtcgtcgcg ccgctatgcc ggggttggcg acgtcatcgt cgggacggtc aaggaggcca
     1081 cccccggcgg ctcggtgaag aagggagagg tggtgcgagc cgttgtggtg cgcaccaaga
     1141 aggagtcgcg tcgtgacgac ggcacctaca tccgcttcga cgacaacgcc tgtgtcctca
     1201 tcgaccccaa ccgtcagccg cgtgggaccc gcatcttcgg tcccgtggcg agagagctgc
     1261 gagacgagcg gttcatgcgg atcgtctccc tagcgcccga ggtgctctga tgatggtcgg
     1321 ccgactactc ctattcccga ccgactttcg ccgagtgtct cccatggctc cgagtcgtcg
     1381 cgtgcctggg gatccaccaa cacgacaagc gtcgccgact tcgatcgccg gcgaaacaga
     1441 cgccggtcgg tcgactgcta ccgcactcgc gaggggcttc attggaggtt tgatatgaaa
     1501 ctcaagaagg gtgacaccgt gatggtcatt gccgggaagg acaccggcaa ggagtcgaag
     1561 gtaacccggg tcctgcctcg caagggcaag gtccaggtgg aaggtgtcaa caccgccaaa
     1621 cgccacacca agccgaccgg tcagaccatg caggggggga tcatcgacaa ggacatgccg
     1681 atcgatgcct ccaacgtgat gatcgtctgt gagaagtgtg gtcccacccg aatcggccat
     1741 cgcatcgacg acaagggcaa caagcaccgc acctgtgtca agtgtggagg tgacctatga
     1801 gcgccaagac cggcaccaag accagcacca agccacgtct caaggctcgc tacgacgacg
     1861 agatccacgc ccagctcaaa gaacagctcg gactggtgaa cccgatggag gttcccaccc
     1921 tcgagaagat cgtcctcaac atgggtctcg ccgaggcgaa ggacaacaag aaggtcatcg
     1981 acgaggcaat cgacgagctg ggtctcatcg caggtcagaa gccccgggtg aacaaggccc
     2041 ggaagtcgat cgccaacttc aagctccgcg aggggatgcc tatcggagtc tccgtgacgc
     2101 tccgcggata tcgcatgtgg gagttcttcg atcgtctcct gtccgtcgcc atccctcgcg
     2161 tcagggactt tcgtggtctc aacccgagag ccttcgacgg tcgcggcaac tacagcgtcg
     2221 gagcttccga gcagctgatc ttccccgaga tcgacttcga ctccgtctca gagaccaggg
     2281 ggatgaacat cacgatcgtg accactgccg acaacgacga ggcaggtaag gccctactcg
     2341 atgcgttcgg gttccccttc cggcgcgtgg aaaccaccgt ctgaggaggt gtgaacagca
     2401 atggctaaga aatcgatgat cgcaaaggcg aacaagaagg cgaagttcaa ggtgcgcgag
     2461 tacaaccgct gccagcggtg cgggcgcccc cgcgccttca tccgtgactt cggaatgtgc
     2521 cgtatttgcg ttcgggagct cgccagccgt ggcgagctgc ccggtgtgag aaaggcttca
     2581 tggtgacaat ggttactgat cccatctcgg acatgctcac ccgcatccgc aacgcggttg
     2641 ccgtgggtca tgagcgggtg gcgatgccgt catccaagct gaaagtgaac atcgcccaga
     2701 tcctcgtcga cgaaggattc atcgatcgct acgaggtgtc cgagaacggt caccgcagcg
     2761 atctcgagtt ggtgttgcgc tacgcggagc gtcggcgtcc agtcatcgag ggactcaagc
     2821 gagtctcccg acccggtcac cgcgtgtaca ggggagccgc cgagcttccc cgggtccaag
     2881 ggggcctcgg cgtcgccgtg gtgtccacga gccagggctt gatgcccgac cgcgaggcgc
     2941 gcaagcgccg tcttggtggc gagatcatct gcgaggtgtg gtgatgagcc gcgtcggaaa
     3001 caagcccgta gacctgcccg atggcgtcga cgtgaagatc gacggcagcg tggtcaccgt
     3061 caagggctcc aagggcgagc tcgcccgcac cttccatgag cggatcgggt tcgacctcga
     3121 cgatggcgtg gtcagcgtca ccaggccgga tgacgaacgt gagtcgcggg ctctccacgg
     3181 tttgagtcgg gcgctcctcg ccaacatggt caccggagtg tcggacgggt tccgcaagga
     3241 gctcgagatc catggtgtcg gctaccgagc atcgatgaaa ggcaccgaca tcgagctcct
     3301 ggtgggtttc agccatcccg ttgtggtcga ggcacccgaa ggggtcactt tcgaggtgcc
     3361 ggaacagacc cgaatcgtgg tctc
//
LOCUS       DASUTA010000129         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1205096, whole genome shotgun sequence.
ACCESSION   DASUTA010000129 DASUTA010000000
VERSION     DASUTA010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205096"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..549)
                     /locus_tag="VFZ80_02645"
     CDS             complement(76..549)
                     /locus_tag="VFZ80_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX6286357.1"
                     /translation="MNSALEALAEQFENVTWDDSHGQDVARVPADEWLEFASAAKEAG
                     FEMCSDVTAVDWLRSRPQRYEVVANLVSMEHKTRLRMTTALGREEPTIATLTAVWPGA
                     GFAEREVYDMFGIVFDGHHDLTRILMPDDWEGHPLRKDFGVGMVPVQFKTSHQVD"
     gene            complement(549..1016)
                     /locus_tag="VFZ80_02650"
                     /pseudo
     CDS             complement(549..1016)
                     /locus_tag="VFZ80_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            complement(1021..1446)
                     /gene="ndhC"
                     /locus_tag="VFZ80_02655"
     CDS             complement(1021..1446)
                     /gene="ndhC"
                     /locus_tag="VFZ80_02655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX6286358.1"
                     /translation="MTLADYAPILVMLVLAAGFVAVSLVVSRFFRPDRPTPEKLAPYE
                     CGIIPEVEPSQRFPVKFYLVAMLFVIFDVEIIFLFAWAAIWDQLGWYGVAAVLIFTFF
                     VIETLMYVWKRGALDWNLHRRARYIHVDEIVAPEVGEAA"
     gene            1515..>2611
                     /locus_tag="VFZ80_02660"
     CDS             1515..>2611
                     /locus_tag="VFZ80_02660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEX6286359.1"
                     /translation="MADTIDIAAVRAQFPALTRTEGSLTAAYLDGPGGTQTPARVVEA
                     MAGVLERGVSNLGGGFGASDEAGQITADARAAMGRMLGADPSTISFGQNMTSITFAMS
                     RALSTTWRAGEAIVVTSLDHDANFTPWTMAAADAGVEVRVAEFDPTTGQLEPSRIADL
                     LDERVRLVAVCLASNSLGSVVDVATVSEAAHQFGALVYVDAVHAAPHLRVDVGTLGCD
                     LLVASAYKFFGPHTGILYGTPEVMASTDAYKVRPAPSDPPGKWETGTQSFESLAGITA
                     AVEYLESLGQDSIADHERILGQRFLDGIAGMTHVELYGVPEMSPDRVATFAVGVAAYH
                     PNEVARRMAEAGVYVWSGHYYAVNVMDRLGVL"
BASE COUNT          480 a          813 c          848 g          470 t
ORIGIN      
        1 ggctggaact gaggctcttc ggttccggcg acccagaggt cgaggatctc cttctcggtc
       61 ttctggtcgg tcatctcaat ccacctggtg tgacgtcttg aactggaccg ggaccatccc
      121 gacaccgaag tccttgcgca gcggatgacc ttcccagtcg tcgggcatga ggattcgagt
      181 caggtcgtgg tgaccatcga agacgatgcc gaacatgtca tagacctcgc gctcggcgaa
      241 cccggccccc ggccacaccg cggtcagagt ggcgattgtg ggttcctcac gcccgagagc
      301 ggtcgtcatg cgaagtcggg tcttgtgttc catcgagacc aggttggcaa ctacttcgta
      361 gcgttgcggc ctggagcgga gccagtcgac agccgtgacg tcggagcaca tctcgaatcc
      421 ggcctccttg gctgcggagg cgaattcgag ccattcatcg gccgggaccc tggccacatc
      481 ctggccgtgg gagtcgtccc aggtgacgtt ctcgaactgc tcggcgagag cttcgagggc
      541 cgaattcatg cgttgactat ctcccccgcc atgatcttgt catgcaggct gaggatgcca
      601 tgcatcaggc tctgcgggcc gggcgggcat cccgggacgt agatgtccac agggaccacc
      661 tgatcgaccc cttgcaccag ggcatagttg ttgaacatcc ccccagtgct ggcgcaggca
      721 cccatcgaga tcacccattt ggggtccggc atctggtcgt agacctgccg gagcacgggc
      781 gccatcttct ggctgacccg tcccgccacg atcatcaggt cggcctggcg gggtgaggcg
      841 cgaaagacct ccatcccgaa gcgagccagg tcgtagtgag cggctccggt ggccatcatc
      901 tcgatggcac agcaggcgag accgaacgtg gccgggaaca tcgagcgggt gcgggcccag
      961 ttggctgcct tctcgaccgt ggtcatcaac aggttgcccg gtggcgtggt cgccatggcc
     1021 ctacgcagcc tccccgactt cgggtgcgac gatctcgtcg acgtggatgt atcgggcgcg
     1081 acggtgcagg ttccagtcga gtgctccccg tttccacacg tacatcaacg tctcgatgac
     1141 gaagaaggtg aagatgagga cggccgccac tccgtaccag cccagctgat cccagatggc
     1201 ggcccaggcg aacaagaaga tgatctcgac gtcgaagatc acgaacagca tggccaccaa
     1261 atagaacttg acggggaacc gttgcgaggg ctccacctcg gggatgatcc cacactcgta
     1321 aggtgcgagc ttctccggtg tgggccgatc gggccggaag aatcgtgaca cgaccagaga
     1381 gaccgcaacg aagccggctg ccagaaccag catcaccaag atgggtgcgt agtcggcgag
     1441 agtcacgttg ctgaggatag ggccgtgctc cggagtgagg taatcagccg gcggcaggct
     1501 tatcgtgccc tgcgatggcg gacacgatcg acattgctgc agttagagcc cagtttcccg
     1561 ctctcacgag gacggaaggg tcgctcactg cggcgtatct cgatggccca ggagggacac
     1621 agaccccagc ccgggtggtc gaggccatgg ccggcgtgct ggaacgggga gtgagcaatc
     1681 tcgggggcgg cttcggagcc tctgacgagg cggggcagat caccgccgat gcccgcgccg
     1741 cgatgggccg aatgctcgga gccgaccctt cgaccatctc cttcggtcag aacatgacaa
     1801 gtatcacctt tgccatgagt cgggcgctga gcacgacttg gcgtgcgggc gaggccatcg
     1861 tggtcacatc actggaccac gacgccaatt tcaccccgtg gacgatggcg gcggccgacg
     1921 ccggggtcga ggtccgcgtg gccgagttcg acccgacgac gggacagctc gaaccgtccc
     1981 gcatcgccga cctgctggat gagcgagtga ggctggtcgc cgtctgtctc gcctccaact
     2041 ccctgggctc tgttgtcgac gtcgccacgg tcagcgaggc agcacaccag tttggagccc
     2101 tggtctacgt cgatgcagtg cacgccgccc cacatctccg agtcgatgtc gggacactcg
     2161 gctgtgatct cctggtggca tcggcctaca agttcttcgg cccccacacc ggaatcctct
     2221 acggcacgcc cgaggtcatg gcatcgaccg atgcctacaa ggttcggccc gcgccgtcgg
     2281 accctcctgg aaagtgggag accgggacgc agagcttcga atcactcgcc ggtataacgg
     2341 ctgcggtcga gtatctcgag tccttgggac aggactcgat cgccgatcac gagcgaatcc
     2401 tcggacagcg gtttctcgac ggaatcgctg ggatgactca cgtcgagctc tacggagttc
     2461 cagagatgag cccagatcga gtggccacct tcgccgtggg cgtggcggct tatcatccca
     2521 acgaggtggc ccggcggatg gctgaagcgg gggtatacgt gtggtcgggc cactactacg
     2581 cggtcaacgt gatggaccgt ctcggcgtgc t
//
LOCUS       DASUTA010000130         3613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1205436, whole genome shotgun sequence.
ACCESSION   DASUTA010000130 DASUTA010000000
VERSION     DASUTA010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205436"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..882
                     /gene="aat"
                     /locus_tag="VFZ80_02665"
     CDS             220..882
                     /gene="aat"
                     /locus_tag="VFZ80_02665"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012917833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX6286360.1"
                     /translation="MPVEPIPSQFDLPDPRTAEGDVVAVGGDLEPGTLLQAYRHGMFP
                     MDLPDGHLGWWSPVERAVIPLNALRVTRSLRQSTRRYFVSVDEDFDAVIRWCAEIDRP
                     NGWITDEIIDAYTRLHELGWAHSIEVWDVDRHLVGGLYGVAIGGLFAGESMFHLARDA
                     SKVALVHLVVVMNRGGRLLDVQWQTPHLESLGAVVIGRDEYLRRLPEALAVPGPFAHG
                     SG"
     gene            930..1787
                     /locus_tag="VFZ80_02670"
     CDS             930..1787
                     /locus_tag="VFZ80_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6286361.1"
                     /translation="MAPAVVAGIDLTTMGRRVADRARLVAEGIGGEVELVHVLEPVAE
                     AMIEPGLARLMREHQIQEARRIADWVADRTSVDVRLDVVKGSPAWELASRAKKVDLVA
                     LGSSTVDAFSGGPVTLRVARMALSDVLIVRRQPRVPYRRILAAVDFSEASRAAVTRAL
                     EMFPEADVTVMYSLPARFDPILSDAGLFTEEMEASRSNRIEAAKDRMLEFTQSWNGDV
                     RTMVTDGPPAQNIDEAVRRRGADLVVVGSRGASATRMVLLGTVPEALVGHAPCDVLIA
                     RSPASFRRP"
     gene            complement(1876..2157)
                     /locus_tag="VFZ80_02675"
     CDS             complement(1876..2157)
                     /locus_tag="VFZ80_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286362.1"
                     /translation="MGCLFLTILLISPRLGVVALWLFTDWVDRAFAGWILPVLGIIFL
                     PWTTGLYVLGFVIGDAAAPWGILGAFIGLFLDIALHAGSATMSRRRRTA"
     gene            complement(2280..>3613)
                     /locus_tag="VFZ80_02680"
     CDS             complement(2280..>3613)
                     /locus_tag="VFZ80_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX6286363.1"
                     /translation="PLAAQEQFCSDVETHVEAIGRYGGLFEDVELTVGEVKTAQQELE
                     PSLEAVEESAEEFRTAVETDPTSGLSIDLVEPETLEAVQAAEEAFAQASDIDDRTPVV
                     DAGIEFNSAAYQLEVAWVRLFVDAGCLEGDARAEAEAQQWVSDYVAAIQTDLRTIGYY
                     QGEVDGIYGPMTIEAVERFQEDSGLPITGLFDPPTQAAMQLALDDRESAQVGALQAIM
                     IATGHYSGPVDGVWSPAVEAALFDLQEDLGVPASGVIDAATLRALEDALAASDEDPVM
                     PTSTPETTPPPADTTTTLAEATTTTVDAATTTTVPAEGGILDVLAEAGQFEQFLAAVE
                     TAGLTDMLSGPGPLTVFAPTDDAFEAAGGLPEDPEALAEVIMYHVVDGALSGFDVQDS
                     ETLLSLQGPEITVGVEQGIITLNGTTAVTIANIAAGNGVVHAVNAVLAPPS"
BASE COUNT          638 a         1141 c         1157 g          677 t
ORIGIN      
        1 ggtctgatct gatattggcc tttgaccatc agggtgtcgg cgaagttcac ggtcaccgat
       61 tcgacctcga ccagaaccga accctggtcg ggcgcgggat caggcaactc cccgacacgc
      121 agagactcga tccccgagta gtcgtcgcat atgagggccc tcatcgaccg cagcctaaga
      181 ccagacacgc caggcacgcg cttctgacac aatcggcatg tgccggttga gccgatcccg
      241 tcccagttcg atctccccga cccgcgcacg gccgaaggtg atgtggtagc cgttggcgga
      301 gacctcgagc cgggaactct gctgcaggcc taccgccatg ggatgttccc catggacctg
      361 cccgacggtc atctcggatg gtggtcaccc gtggagagag cagtgattcc cttgaacgcc
      421 ctccgggtga ctcgttcgtt gcgacagtcg accaggcgct atttcgtgtc ggtggacgag
      481 gacttcgatg ccgtcatcag gtggtgtgcc gagatcgatc ggcccaacgg ctggatcacc
      541 gacgagatca tcgacgccta cacccgtctc cacgagctcg ggtgggctca ttccatcgag
      601 gtctgggatg tcgaccgcca tctggtcggt ggcctctacg gcgttgcgat cggcggtctc
      661 ttcgccgggg agagcatgtt ccatctggct cgcgacgctt ccaaagtcgc actcgttcat
      721 ctcgtggtcg tgatgaaccg aggcgggcga ctcctcgatg tgcagtggca gacaccgcat
      781 ctcgagtccc tgggagcggt ggtcataggg cgcgacgagt atctccgccg actccccgaa
      841 gccctcgcgg tgccggggcc attcgcacac ggcagtggtt agagacgctg cggcgacagt
      901 agagtcgccg gcgtgctcca gggagaagga tggctccagc cgtagtagcc ggtatcgatc
      961 tgacaaccat gggtcgccgg gtcgcagatc gagctcgttt ggttgctgag ggaatcggtg
     1021 gagaggttga gttggtgcat gtcctcgagc ctgttgccga agccatgatc gagccgggac
     1081 tggccaggct catgcgggaa caccagatac aggaagcccg caggatcgcc gattgggtcg
     1141 ccgaccgaac gtctgtggac gtcaggctgg atgtggtcaa agggtctccc gcctgggaac
     1201 tcgcctcccg ggccaagaag gtcgatctgg tcgctctggg ctcgtcgacg gtcgatgcct
     1261 tctccggagg tccggtgacg ctccgggtcg ccaggatggc gttgagtgac gttctcatcg
     1321 tgcggcgaca gcctcgggtt ccgtaccgac gcattctcgc cgcggtcgac ttctccgagg
     1381 cgtcgcgagc agctgtgacc cgggccctcg agatgtttcc cgaagccgat gtgacagtga
     1441 tgtactcgtt gcccgcgaga ttcgacccca ttctctccga cgccggtttg ttcaccgagg
     1501 agatggaggc ctcccggagc aatcggatcg aggcggccaa ggaccggatg ctcgagttca
     1561 cccagtcctg gaacggggac gttcgcacca tggtcaccga cggtccacct gcccagaaca
     1621 tcgacgaagc cgtgcgcaga cgtggcgccg acctggtcgt ggtgggtagc aggggtgcct
     1681 cggccacccg catggtgttg ctgggaacgg tgcccgaggc tctcgttggc catgccccct
     1741 gcgatgtgct catcgccagg tcgccggcga gttttcgacg tccctgagac ccttctgtga
     1801 aagtctgtca cccgtggccc gggccaagcg ctcacatgac ggttcgaatt ggaccgcgag
     1861 accctttccg gcaggtcagg ccgtgcggcg ccgtctgctc atggtcgccg accctgcatg
     1921 gagggcgatg tcgagaaaga gcccgatgaa ggctcccagg atgccccagg gcgcggcggc
     1981 gtcaccgatg acgaatccga ggacgtagag gccagtcgtc caggggagga agatgatccc
     2041 gaggaccgga agaatccaac cggcgaacgc tcgatcgacc cagtcggtga acaaccagag
     2101 ggcgacgaca cccagcctcg ggctgatcaa caagatggtc agaaatagac agcccatcac
     2161 cgaatcccgg tctctgcccg agtcatttcg cgttcccttt cctcagtccc cccaatcaag
     2221 cacaaagagt aggcattggc atcgccccga tcgggctatg aatgcaacgt atgtgcctgt
     2281 cacgatggtg gcgcgagaac cgcattgacg gcgtggacca cgccgttgcc ggcggcgatg
     2341 ttggcgatcg tcacagccgt ggtcccattc aaggtgatga ttccctgctc gactccgaca
     2401 gtgatctctg gcccttgcag actcaacagt gtctctgagt cttggacgtc aaagcctgac
     2461 agcgctccat cgacgacgtg atacatgatc acctccgcca gagcctcagg gtcctcgggc
     2521 agaccgcccg ccgcctcgaa agcatcgtcg gtgggtgcga agacggtcaa tggccccggg
     2581 ccggacagca tgtcggtcaa gcccgctgtc tccacggcgg cgaggaattg ctcgaactgg
     2641 ccggcctcgg cgaggacatc gaggattcca ccttctgcag gcacggtggt cgtcgtagcc
     2701 gcatcgactg tggtcgtggt ggcttcagcg agggtggtgg tcgtgtccgc gggcggggga
     2761 gtggtctccg gcgtgctcgt gggcatcacc gggtcttcat cggacgccgc cagggcatcc
     2821 tccaatgcac gcaaggttgc tgcgtcgatg accccagagg cgggcacccc gaggtcctct
     2881 tggagatcga agagcgccgc ttccacggcg ggcgaccaga ccccgtctac agggcccgag
     2941 tagtgaccag tggcgatcat gatcgcctgt aaggctccga cctgggcaga ctccctgtca
     3001 tccagggcga gttgcatcgc cgcttgcgtc ggtgggtcga agagccccgt gatcggtaag
     3061 ccgctgtcct cctggaatcg ctcgacggct tcgatcgtca tggggccata gatcccgtcg
     3121 acctcaccct ggtagtagcc gatggttcgc agatccgtct ggatcgcagc cacgtaatcc
     3181 gagacccatt gctgggcttc ggcttcggct cgagcgtcgc cttcgagaca accggcgtcg
     3241 acgaagagac gaacccaagc cacctccagc tggtaggcgg cagagttgaa ttcgatccca
     3301 gcatcgacga caggggtccg gtcgtcgatg tcactcgcct gggcaaaggc ctcctcagct
     3361 gcttgcactg cctccagcgt ctcgggctcg acgagatcga tgctcagccc ggatgtcgga
     3421 tcggtctcga cggcggtcct gaactcctcc gctgactcct cgaccgcctc caagctgggc
     3481 tcgagctctt gctgagccgt cttcacctca ccgaccgtga gctcgacgtc ctcgaagagg
     3541 cctccgtacc gaccgatcgc ctcgacatgt gtctcgacat cggagcagaa ctgttcctgg
     3601 gcggcgagcg ggt
//
LOCUS       DASUTA010000131         5894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_428731, whole genome shotgun sequence.
ACCESSION   DASUTA010000131 DASUTA010000000
VERSION     DASUTA010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428731"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VFZ80_02685"
     CDS             <1..245
                     /locus_tag="VFZ80_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286364.1"
                     /translation="GDVSWVKNARAKPSVTLRSGGTSRKLILVDVTGQAAHVVKAYWD
                     REKFSRPYMDVPGDPEVSDFAKAADRFPVFKVEAQA"
     gene            291..1622
                     /locus_tag="VFZ80_02690"
     CDS             291..1622
                     /locus_tag="VFZ80_02690"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX6286365.1"
                     /translation="MATTTQEIPQVTYVLDTCVLLADPHSLLRFDEHDVVLPLVVVEE
                     LDRKKTRLDEVGTNARGAIRLLEELGASTNGGMREPVRLPGGGTLRIELNGIVSDKLP
                     SVLDPTSPDHRILATCLNLVETDDAVLVTKDAALRIKGAQLGVPVQDYRADTAQVDQL
                     HSGVVEHRVSAETIDRLFDEGKLEIEGVEAPINEFVVLRHGSSRSALARIVQAEPTVV
                     VERMPTHVRAFGVEPKNLRQTVALHQLLDPDIPAVSLMGMAGTGKTFLSLAAALDQVL
                     EQGRYRRVSVYRPLVAVGRQEVGYLPGDLSEKLAPWMAAVHDNLYALFSDAGVDGARS
                     AIEELVDRGELEMAAVTYLRGRSITGEFVIIDEAQNLELSTLKVILTRMSRDSKVVFC
                     GDLSQVDNPYISPYGGMAALIEKFKDSSLFAHVSLERTVRSPLAEMAARVL"
     gene            1671..1850
                     /locus_tag="VFZ80_02695"
     CDS             1671..1850
                     /locus_tag="VFZ80_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286366.1"
                     /translation="MTEPDRPQRNSGMLVPIGSVLGAGIGAAIGAAFGELSTGLWIGA
                     VIGVGLGMLGMYFSS"
     gene            complement(1851..4412)
                     /gene="topA"
                     /locus_tag="VFZ80_02700"
     CDS             complement(1851..4412)
                     /gene="topA"
                     /locus_tag="VFZ80_02700"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX6286367.1"
                     /translation="MPKSLVIVESPTKARTISSFLGSEATVESSMGHIRDLPRSASEI
                     PKKVKDEDWARLGVNVENDFEPIYVVPKERRSQVKKLKDLLADVEELYLATDEDREGE
                     AIAWHLVEVLKPKVPVKRMVFHEITRPAIEEAFSNPRDLDERLVSAQEARRILDRLYG
                     YEVSPVLWKKVQPKLSAGRVQSVAIRIVVERERERMAFVPAGYWSIEGSFAPADDLDK
                     TFDATLLRVDEHKVATGKDFDADGKARDDVVVLDQESATTLATSLQDSSFRVRSVESK
                     PYTRKPYPPFRTSTLQQEAGRKLRYSSSRTMSVAQRLYENGYITYMRTDSVELSDSAI
                     SASRSQIAKLYGEEYLSPSPRKYSGKQAKGAQEAHEAIRPAGEFQTPEQVAKDVGPAE
                     AALYDLIWKRTVASQMADAKGVSVSVRLAATASSGEDALFGASGRTITFPGFLRAYVQ
                     GSDDPESELDDREKILPPLDEGDELAVERLEPTGHETRPPARYTEASLVQKLEELGVG
                     RPSTYASIMTTIQDRGYVFKKGSALVPTFTAFGVVGLLEKHFPDLVDYEFTAEMETDL
                     DRIAGGEEESIPWLSAFYFGHDGLKEKVATRLDEIDPREINSILIGEDDTGAEIVARV
                     GRYGPYVERGDERGSIPDDLAPDEVTVEKAIELIEEQNKGDKIFGTDPETGLTVYGKT
                     GRFGPYIQLGEQEEGSKVKPKRASLFQSMTMDELGLDDALRLLSLPRTVGTDAEGVEI
                     LALNGRYGPYIQRGDDRRSLESEQQLFTLSEEDALQLLAEPPRRRGQRSSADVKELGE
                     DPVSGKKVTVKSGRYGPYVTDGDVNASLRQGDSPDKITMERAAELLQARRDRLGK"
     gene            4492..5079
                     /gene="tmk"
                     /locus_tag="VFZ80_02705"
     CDS             4492..5079
                     /gene="tmk"
                     /locus_tag="VFZ80_02705"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX6286368.1"
                     /translation="MSKYVALEGVDGSGKSTVARALLEALDRRGIEAILVREPGGTAV
                     GEAVRSLLLDSDALDDWAEVFLFAAQRSELARDVIEPALEAGTWVISDRSYYSSIAYQ
                     GRARGLGEDRVRAINETGLAGVIPDFVFVLDVDPGVALDRQDSPDRIGREGIEFQKAV
                     RDSYLDLAEHEDKVTILDGGLTVEEIISQILEVVS"
     gene            5085..>5894
                     /locus_tag="VFZ80_02710"
     CDS             5085..>5894
                     /locus_tag="VFZ80_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286369.1"
                     /translation="MFDDIIGHSGVVELLEAELSRPAQAYLFVGPQSVGKEAVALRFA
                     AGLLGGIEDERARRLALAAQHPDLRVLEPEGATSLGVDQAREVVARASLTPVEAERSV
                     FLLPDAGAMTDQAANALLKTLEEPSAEVVFLLVAESEDDFPPTVASRCRTVHMGRVPQ
                     AELVQALMERGLGDEAATGVAVVSGGRPGLALALMSQPEVARFRELWLSIPGQVSPHP
                     GDGQRLATVVLEELSPLVEDSVPDDLPKDKAQRARRRAELSLLVSGLEILAS"
BASE COUNT          988 a         1829 c         1894 g         1183 t
ORIGIN      
        1 acggcgatgt ctcgtgggtc aagaacgcga gggccaaacc ttctgtgaca ctcaggagcg
       61 gcgggacatc gaggaagctg attctggtag acgtgaccgg acaggccgcc catgtcgtca
      121 aggcctactg ggacagggag aaattctctc gcccctacat ggacgtgccc ggtgaccccg
      181 aggtatccga cttcgcgaag gcggcggatc ggttcccggt cttcaaggtc gaagcacaag
      241 cctgaatacg atgcttgtaa ttctgcgtcg gatgggtgat gctggtgccc ttggcgacca
      301 caacccagga gatcccccag gtgacctacg tcctcgacac ctgcgtccta ctcgccgacc
      361 cccactctct cctccgcttc gatgagcacg acgtcgtcct gccactcgtg gtcgtggagg
      421 agctcgaccg caagaagacg cggttggacg aggtcggcac caacgcaaga ggagcgatcc
      481 gcctcctcga agagttgggg gcatcgacca acggaggcat gcgtgagccg gtgaggctcc
      541 ctggtggcgg cacgttgcgg atcgagctga acggcatcgt ctcggacaaa cttccctccg
      601 tcctcgatcc cacgagcccc gatcaccgga ttcttgcgac ctgcctcaac ctggtcgaga
      661 ccgatgacgc cgtgctggtg accaaagatg ccgcactgcg catcaagggt gcccagctcg
      721 gcgtccctgt ccaggactat cgggcggata cggcgcaggt ggatcagctc cattccggag
      781 tggtcgagca ccgggtcagc gcggagacga tcgaccgtct cttcgatgaa gggaaactcg
      841 agatcgaggg agtcgaggct cctatcaacg agttcgtcgt gctgcgtcac ggctcgtcgc
      901 gatccgctct ggccaggatc gtccaagccg agccgacggt ggtcgtggag cgaatgccaa
      961 cacacgtgcg ggccttcggg gtcgagccga agaatctccg acagaccgtc gcgctgcatc
     1021 agctcctcga ccccgacatt ccggcggtgt ccctcatggg catggccggg accggcaaga
     1081 cgttcttgtc ccttgctgcc gctctggacc aagtcctcga gcaaggccgc taccgaaggg
     1141 tctcggtcta ccgtccgctc gtcgccgtag gtcgacagga ggtggggtac ctgcctggcg
     1201 acctcagtga gaagctggct ccgtggatgg ctgccgtgca cgacaacctc tatgcgctgt
     1261 tctccgatgc cggcgtcgat ggggcacgtt cggcaatcga ggagctggtc gataggggcg
     1321 agctcgagat ggctgcggtc acctaccttc gtggtcgctc cataaccgga gagttcgtga
     1381 tcatcgacga ggctcagaac ctggagctct ccaccctgaa ggtgattctc actcgcatgt
     1441 ctcgtgattc caaggtcgtg ttctgtggcg atctctccca ggtggacaac ccctacatat
     1501 ccccgtacgg gggcatggcc gcactgatcg aaaagttcaa ggactcgagt ctcttcgctc
     1561 acgtctctct ggagcgcacc gtgcgctcac ctctcgccga aatggcggcc agagtcctct
     1621 gatgactgaa gccgacatcg cgagggaagg cgtgtgtagg ttgccagttc gtgactgaac
     1681 cagacagacc tcagagaaac agcgggatgc tcgtgccgat cggctccgtt ctcggcgccg
     1741 gcatcggtgc cgccatcggg gcggctttcg gtgagttgag cacaggcctt tggatcggcg
     1801 ccgtcatcgg ggtcgggctc gggatgctcg ggatgtactt cagcagctag ctacttcccg
     1861 agacggtccc gccgcgcctg aagcagctcg gctgcccttt ccatggtgat cttgtccggc
     1921 gagtcaccct gtcggagtga cgcattcaca tcaccgtcgg tgacataggg gccgtaacgc
     1981 cctgacttga cggtcacctt cttgcctgac accgggtcct cgccgagttc cttgacgtcg
     2041 gcgctgctcc tctggcccct ccgtcgcggt ggctccgcga gaagctgcaa cgcatcctct
     2101 tcgctcaacg tgaacagctg ttgctctgac tcgagactgc gtcggtcgtc tccacgctgg
     2161 atgtaaggcc cgtaacggcc gttgagagca aggatctcga cgccctcggc gtcggttcca
     2221 accgtccgcg gaagggatag gagtcgaagt gcatcgtcga gacccagctc gtccatggtc
     2281 atcgactgga acagcgaggc acgctttggc ttgaccttcg acccctcctc ttgctcgccg
     2341 agctgtatgt atggtccaaa acggccggtt ttgccataga cggtcagacc ggtttccggg
     2401 tcggtgccga agatcttgtc tcctttgttc tgctcttcga tgagctcgat tgccttctcg
     2461 acagtcactt cgtcgggggc caggtcgtcc ggaatcgaac cgcgctcgtc tccgcgctcg
     2521 acgtaggggc cgtagcgccc gacgcgggcc acgatctcgg cgcccgtgtc gtcttcgccg
     2581 atgagaatgg agttgatctc acgggggtcg atctcgtcca gcctcgtcgc caccttctct
     2641 ttgagcccgt catggccgaa gtagaaggcg gacagccacg ggatcgactc ctcctcacct
     2701 ccggcgatcc ggtcgaggtc ggtctccatc tcggccgtga actcgtagtc gacgaggtcg
     2761 gggaagtgct tctcgaggag accgaccact ccgaaggcgg tgaatgtcgg caccagggcc
     2821 gagccctttt tgaagacgta gccgcggtcc tgaatcgtcg tcatgatcga ggcgtatgtg
     2881 gagggtcggc ccacccccaa ttcctcgagt ttctggacga gggatgcctc ggtgtagcgc
     2941 gccgggggcc gggtctcgtg gcccgtcggc tcgagtcgct cgacggcgag ttcatcgccc
     3001 tcgtcgagtg gcggcaggat cttctcgcgg tcatccagct cggactcggg gtcgtcggag
     3061 ccctggacgt acgcccgcaa gaaaccggga aaggtgatgg ttcgacccga cgcaccgaaa
     3121 agcgcatcct ctcccgacga ggctgtcgca gccaatctca ccgatacgga cacgcccttg
     3181 gcgtcggcca tctgtgatgc cacggtgcgc ttccagatga ggtcatatag ggctgcttcg
     3241 gctggcccga cgtccttggc cacctgctct ggcgtctgga attcgcctgc gggtcgaatc
     3301 gcctcatgag cctcctgggc gccctttgcc tgcttgccgg agtacttgcg tggtgagggt
     3361 gagaggtact cctccccgta caacttggcg atctgagatc tcgaagcgct gatcgcagag
     3421 tcagagagct ccaccgagtc ggtacgcata taagtgatgt agccattctc gtagagacgc
     3481 tgagcgaccg acatggtgcg cgacgacgag tagcgaagct tgcgtcccgc ttcttgttgc
     3541 agcgtcgagg tcctgaatgg gggatagggc ttgcgtgtgt atggcttgga ttcgaccgaa
     3601 cgcacccgga acgaagagtc ctgaagactc gtagccagcg tggtggccga ctcctgatcg
     3661 agcacgacga cgtcgtctct cgccttgccg tcggcgtcga agtctttccc tgtggcaact
     3721 ttgtgctcgt cgaccctgag gagggtggcg tcgaaggtct tgtccaggtc gtcggctggg
     3781 gcgaacgagc cttcgatgga ccagtaaccg gcaggaacga acgccatccg ctctcgttcc
     3841 cgctccacca cgatgcggat cgccacgctc tgcacgcggc cggcggagag tttcggctgg
     3901 accttcttcc acaagaccgg cgatacctcg tacccataga ggcgatcgag gatgcgccgt
     3961 gcctcttgtg ccgagaccag cctctcgtcg agatctcgtg ggttcgagaa ggcctcctcg
     4021 atcgccggcc tggttatctc gtggaagacc atgcgtttga cggggacttt gggcttcaac
     4081 acctcgacca ggtgccaggc gatggcttct ccctcgcgat cctcgtccgt ggcgaggtag
     4141 agctcctcga catcggctag gagatccttc agcttcttga cctgactcct gcgctccttg
     4201 ggaaccacgt atatgggttc gaagtcgttc tcgacgttga ccccgagacg cgcccagtcc
     4261 tcgtctttga ccttcttcgg gatctccgag gcggaacgag ggaggtcgcg aatatgcccc
     4321 atcgacgact cgacggtggc ctccgaaccc agaaacgagg agatggttcg ggccttcgtc
     4381 ggcgattcga caatgacgag actcttgggc attgctgggc agttgacacc agatcggggg
     4441 gttatgtcaa tctctgccac cgtgtcccaa ccgatggtta ccgtgatgat tgtgagcaaa
     4501 tacgtcgcct tggagggtgt tgacggcagt ggcaagagca cagttgcccg ggcccttctc
     4561 gaggcgctcg accgccgcgg catcgaggcg atcctggttc gggagccggg tggcacggcc
     4621 gtgggtgagg cggtgcgctc actccttctc gactccgatg cgctcgatga ctgggccgag
     4681 gtctttctct tcgccgccca gcgctccgag ctggcacgag atgtgatcga gccggcactc
     4741 gaggcaggca cctgggtgat ctccgaccgg agctactact cgtcgatcgc ctaccagggc
     4801 agggcgcgag ggctcggcga ggacagggtt cgagcgatca acgagaccgg gctcgcgggc
     4861 gtgatccccg atttcgtgtt cgtgctcgac gtcgatccgg gtgtcgcgct ggatcgacag
     4921 gattctcctg atcgcatcgg cagggaaggc atcgagtttc aaaaagcggt gcgagactcc
     4981 tatctggatc tggccgagca tgaggacaag gtcacgatcc tcgatggtgg tctcacggtc
     5041 gaggagatca tctcgcagat cctggaggtg gtctcgtgag cgggttgttc gacgacatca
     5101 tcggccacag cggagtcgtc gagctactcg aggcggagtt gtcccgaccg gcccaggcgt
     5161 atctgttcgt tggtccccag tccgtgggca aagaggcggt cgctctgcga ttcgccgccg
     5221 gcctgctcgg ggggatcgag gacgagcgtg cccgtcggct cgccctggct gcccagcacc
     5281 ccgacctgag agtgctcgaa cccgaggggg cgacgagtct cggtgtcgac caggcgcgcg
     5341 aggtcgtggc ccgcgcttct ctgactccgg tagaagcgga gcgctcggtc tttctgcttc
     5401 ccgatgcggg ggcgatgacc gaccaggcgg cgaacgcgct tctgaagacg ctggaggagc
     5461 catccgctga agtggtgttt cttctcgtcg ctgagtccga ggacgatttc ccacccacgg
     5521 tggcttctcg ctgtcgcacc gtgcacatgg ggcgggttcc ccaggccgag ctcgtccagg
     5581 ccctgatgga acgtggtctc ggtgacgagg cggccaccgg ggtcgcggtt gtctcaggtg
     5641 ggcgtcccgg tctcgcgctg gctctgatga gccagcccga agttgcccgg tttcgagagc
     5701 tgtggctgtc gatcccgggt caggtctccc cccaccccgg cgatggccag cgtctggcga
     5761 cggtcgtgct cgaggagttg tcgccgctgg ttgaagactc ggtccccgac gacctcccca
     5821 aggacaaagc acaacgagcc cgtcgtcgcg ccgagctttc gctcctggtg agcgggctcg
     5881 agatcctggc gtcc
//
LOCUS       DASUTA010000132         3322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1725156, whole genome shotgun sequence.
ACCESSION   DASUTA010000132 DASUTA010000000
VERSION     DASUTA010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725156"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..352)
                     /locus_tag="VFZ80_02715"
     CDS             complement(134..352)
                     /locus_tag="VFZ80_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286370.1"
                     /translation="MIIFDAVVKDLRRKGVRLKDYELPGYPTQDRVIADKGTRWLGSK
                     TAKATPLKSTNSSVETTSPPGNVDDMIA"
     gene            813..1037
                     /locus_tag="VFZ80_02720"
     CDS             813..1037
                     /locus_tag="VFZ80_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286371.1"
                     /translation="MTTDPGLPGHRLLERCEACGVDFDHDVARCPECGERTAIGRRRA
                     ITVAIAIVVTLVVLAGLWAAVVLPSSPITT"
     gene            complement(1388..1975)
                     /locus_tag="VFZ80_02725"
     CDS             complement(1388..1975)
                     /locus_tag="VFZ80_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286372.1"
                     /translation="MELYERRDELVAERTALIRSMRGVRSLFVATFAEELEERWELAV
                     SAWLRAHQSPIDERHLGAARRELDVLYSKAREVANSELSGPEVWGHEANETEMSRKRA
                     YAPEPDGTWTHVSPAMERAERKAIQVLERRFVPDDEPIQDWIPGTPSGPLRDILETYA
                     ESFTSLQKVNIALERVRNQLPRPRPNLAAARLSSD"
     gene            complement(2135..>3322)
                     /locus_tag="VFZ80_02730"
     CDS             complement(2135..>3322)
                     /locus_tag="VFZ80_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012182306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6286373.1"
                     /translation="GRGDVIDNGVVVLDDTTISFAGAATDAPDTPHAEVVKTETVMPG
                     MWECHGHFIGIYTANIDEAFTTRPQLAAMRVTKDAGAVLDAGFTSVREMGGLGSFLGR
                     AVEEGVVIGPNVYGSGSMLSMTAGHGDTHSLDLDVARLLHKIWGEDNIVDGPDQCRAG
                     VRRMLRLGAKVIKVHASGGVLSELDDPHLPQFSKAELEAIVDEATRMERLVGAHCHGK
                     RGIMAALEAGIKTIEHGTFIDEECADAMVETGAILVPTRFIIDLLMREGDERGMPEYA
                     KKKIEMTAEAHADGMALAIEKGVKIALGTDIWGTGIWGQNGKELSLMVDCGMTPLQAI
                     EMATANGPATLGPQAPNSGQLKTGMDADVICVTGDPSADVTILADTANVTHVFRGGTR
                     YKG"
BASE COUNT          594 a         1050 c         1000 g          678 t
ORIGIN      
        1 ttggattctg catgaatccg gcactgcccc gatgaacgag tccgacacgt gtgaacatgt
       61 gagggatgga ttcgaggttg ccaccggcat cgaaccgacc gcttcttgcc cctgaatatc
      121 gcgtgtgcaa ggactacgca atcatgtcgt caacgttgcc cggtggagac gttgtctcca
      181 ccgacgaatt cgttgatttc aatggtgttg ccttcgctgt cttcgaacca agccatcttg
      241 tacccttgtc tgcgatgaca cggtcctggg ttggatagcc tggaagctcg tagtctttga
      301 gcctcacacc cttgcggcgc aggtccttca ccacggcatc gaagatgatc acatcaaacc
      361 cggttgccgt actcttacac gttccagcga aggggctcgg gacgaagaga agccacgtct
      421 cctcgctaca gcagtaaagg agtctcgacc gggcttggcg agcaccagat cggggaaacc
      481 ggcgttgcct tggatcgggg tcgtccaaga cccatcacgc cgtcggcagg gtcggatggc
      541 tcagaccgcc cgctacgggc cttagacggc ttccgcatgt gcacgatctg tgcacgattc
      601 tcctgaaaca ctcgaaagac ccaatggcta cagagtttct gtggggttga cctcagtcct
      661 cacacgcgag aggtcagtgg ttcaggtcca ctacggccca caaggggacc tgggaggaca
      721 ggcggtcgtc acattcccga ccctttcagc actcccagcg atggcggcgg atccagcacc
      781 tcgatgtcag gcaattagct aggttttgac ctgtgacaac agatcctggt ttgcccggac
      841 accggcttct cgagagatgt gaagcatgcg gcgtcgactt cgatcacgac gtggcgagat
      901 gccccgaatg cggagagcgc accgcgatag gaagacgcag ggcgataacg gtggcgatag
      961 caatcgtggt cactctcgtg gtgctcgcgg ggctctgggc cgcagtcgtc ctacccagta
     1021 gtcccatcac gacctgagcc attcggcacg cgattctcat gagacgatcg tttactcagg
     1081 tcgaccgatc gagtagcgtg tcgtcgcttc ccgaggtcaa ccctgcttcg agacatagac
     1141 aaatgtctgc acggccgcgg cgcgcaagcc cgactctgca aagggttgac cgaacctcac
     1201 ggccgaagac catgctccct tggacgagga ccccaggcct tcagccttgt caacgattgc
     1261 acgttcgtcg agaagagtgc agagggttct gaggccctcg tccaagggag cagggggaac
     1321 gagtctttca cgcctgctcg cctgatcgaa gtgtaacgat gcggtcttcg gtagcccggc
     1381 agtcgtatca gtcggaggac agccgggccg ctgccaggtt gggccggggt ctcggaagct
     1441 ggtttctcac ccgctccagg gcgatgttca ccttctgcag gctcgtgaag ctctccgcat
     1501 acgtctcgag gatgtcacgc agcggtcctg aaggcgtgcc ggggatccaa tcctgtatcg
     1561 gctcgtcgtc agggacgaac cgccgctcca acacctgaat ggccttgcgc tcggcacgtt
     1621 ccatggcagg tgacacgtgg gtccacgtcc catcgggttc gggcgcataa gcccgcttgc
     1681 gggacatctc ggtctcgttg gcttcatgac cccagacctc aggtccactc agctcactgt
     1741 tggcgacctc acgtgccttc gaatagagga cgtcgagctc gcgccgtgcg gccccgagat
     1801 gcctctcatc gattgggctt tgatgagcgc ggagccatgc actcaccgcc agctcccagc
     1861 gctcctcgag ttcctcggcg aaggtggcga cgaaaaggct gcggacccct cgcatgcttc
     1921 gtatgagcgc ggtccgctcg gcgacgagct catctcgtcg ttcgtacaac tccatcgaca
     1981 tgaccatctc ctgttgcttg ggctcgcttg caggaagtga ccatcggaac cgtgcaagag
     2041 acgatccact tctctctcag gtgtgttcat cgaccgacgc cttgttgggc cttagccccc
     2101 gtcgggcggt tctcttaggc cgttcgaccc aaactcaacc cttatagcgg gtaccgcccc
     2161 tgaatacgtg ggtgacattg gcggtgtcgg cgaggatcgt cacatcggcg gaggggtcgc
     2221 cggtgacgca tatgacgtcg gcgtccatcc ccgtcttcag ctgtccactg ttgggggcct
     2281 gcggtcccaa cgtcgccggg ccgttggccg tggccatctc tatcgcttgg agcggcgtca
     2341 tcccgcaatc caccatgaga gacaactctt tgccgttctg accccagatg ccagtgcccc
     2401 aaatgtcggt gcccaaagcg atcttgacgc ctttctcgat cgccagcgcc atgccgtcgg
     2461 cgtgggcctc ggctgtcatc tcgatcttct tcttggcgta ctcgggcatc ccgcgctcat
     2521 cgccctctct catcagcagg tcgatgatga aacgagtcgg gacgagaatc gcaccggtct
     2581 ccaccatggc gtcggcgcac tcttcgtcga tgaacgtccc gtgctcgatg gtcttgatgc
     2641 cggcttcgag agcggccatg atcccgcgct tgccgtgaca gtgtgccccc accaaacgct
     2701 ccatgcgggt ggcctcatcg acgatggcct cgagctcggc cttcgagaac tgggggagat
     2761 gagggtcgtc gagctcgctc aataccccac cggaagcatg gaccttgatg accttcgccc
     2821 cgagacggag catgcggcgc acgccagcgc ggcactgatc gggtccatcg acgatgttgt
     2881 cctcgcccca gatcttgtgg agcaggcggg ccacatcgag gtcgaggctg tgagtgtcgc
     2941 cgtgcccggc tgtcattgag agcatggagc cggacccgta cacgttggga ccgatgacca
     3001 cgccttcttc gactgccctg cccaggaacg agccgaggcc gcccatctcc cggaccgagg
     3061 tgaatccggc gtcgagaacc gctccggcat ccttggtcac ccgcatcgcc gccagctgtg
     3121 gccgggtggt gaaggcttcg tcgatgttgg cggtgtagat gccgatgaag tgcccgtggc
     3181 attcccacat tcccggcatc acggtctcgg tcttgaccac ctcggcgtgg ggggtgtcgg
     3241 gggcgtccgt ggccgccccg gcaaaagaga tcgttgtgtc gtccaggacg accaccccgt
     3301 tgtcgatgac gtctccgcgc cc
//
LOCUS       DASUTA010000133         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1855855, whole genome shotgun sequence.
ACCESSION   DASUTA010000133 DASUTA010000000
VERSION     DASUTA010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1855855"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            375..1049
                     /locus_tag="VFZ80_02735"
     CDS             375..1049
                     /locus_tag="VFZ80_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286374.1"
                     /translation="MSAVVTTATSRQAGRESKTTATRIVVSAFGALAALAGVEHGVGE
                     LLQGSVRPPSVVIESWPETAGFEILSGEPAMTLVPNMAVTGVLAIIAALAVGVWAVWF
                     IQRPHGGLILIALSVVLLLLGGGFGPPAVATIVGVAATRIGRERRRDPGFLGSALARA
                     WPWLLGAGLVGYLGLVPGMVLVSRTTGFDNQYVVSGLMFVAFAGLILSLIAARAYDDL
                     TVGTSS"
     gene            1049..1798
                     /locus_tag="VFZ80_02740"
     CDS             1049..1798
                     /locus_tag="VFZ80_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286375.1"
                     /translation="MDKAGSIDTRRIGSALSSSEGLGPWRGVLRVGGWAALASVVLTF
                     VQVWIYVQWPPPETVEGFYALFAERPFLGLLSMDLLYIVNNAIVLLVYLALFAVLRHA
                     YPSTVAIGLLLGAMGMAAYMASNTGFEMLSLANAYATADATGRVALIGAGEAMLAVFE
                     GTAFDIYYVLSAIALFLFAGSMLRSRLFSRATGFWGLAAAVLMIVPSTAGTVGMVFAL
                     GSLIPWVAFSVLTGRQLLYLGSTGRGSEAGL"
     gene            1825..>2152
                     /locus_tag="VFZ80_02745"
     CDS             1825..>2152
                     /locus_tag="VFZ80_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6286376.1"
                     /translation="MIDRSPAEVFDFCSDLRNELVWNPTSEYVEKLTDGPVGVGTRYA
                     AKWSNTRRVIVEVSGFDPSTTWETRSRAGGLTITVQGRLSDLAPGTRYETTVGVEARG
                     LAHLYAP"
BASE COUNT          324 a          619 c          748 g          461 t
ORIGIN      
        1 cgaggcggct caggacacga tctgacagtg agcgcccaca ccagcaaacg atggtcccgc
       61 cccccgccga ttcccgaagc gtggagggcc aggagtgaca cggacatcct aggtcctgtt
      121 tcccgaaagg acctgggccg aggtgtcggt ggccgacgaa gcaccaatgg agtgaagagg
      181 tggcgtcact gccgccgtgc cgaggatcga tggaatccga tgagagccgt ggtgaccacc
      241 tttcgtttcg agccggtgcc tcgtgctcga tcccgtaccc gcttttcgga ctgttggctg
      301 gctggggagg ggcgaaagcc cctgggtggt gcgctcgcgt tcccgtagag agggagagtg
      361 acgattggag tcggatgagt gctgttgtga ccaccgcgac gtctcgacag gcggggaggg
      421 agagtaagac cacggcgact cggatcgtgg tttcggcgtt tggtgccttg gcggcgttgg
      481 cgggcgtaga gcacggggtt ggtgagctcc tgcagggctc ggttcgccct ccgtcggtgg
      541 tcatcgagtc gtggcctgag actgccggat tcgagatcct gtccggtgaa ccggcgatga
      601 cgctggtgcc aaatatggct gtcaccggag ttctggcgat catcgccgca ttggcggttg
      661 gggtctgggc ggtctggttc attcagcgac cccatggcgg gctgattctg atcgccctct
      721 ccgtcgttct gctgttgctc ggcggagggt tcggaccacc ggcagtggcg accatcgtcg
      781 gtgtggcggc gaccaggatc ggtagggaac gtaggagaga ccccggattc cttgggtctg
      841 ccttggccag ggcgtggcct tggttgctgg gtgccgggct ggtcggctat ctcggactgg
      901 tccccgggat ggtgctggtc agccggacga ccggattcga caatcagtac gtggtctcgg
      961 gactcatgtt cgttgcgttc gccggcctga tcctctcgct gatcgctgcc cgtgcatacg
     1021 acgacctgac ggtggggacg tcgagctgat ggacaaagcc ggttccatcg atacgaggcg
     1081 aatcgggtcg gccctgtcat cctcggaggg gctcgggcca tggcgcggcg tgctcagggt
     1141 cggggggtgg gcagccctgg cgtcggttgt cctcaccttc gtccaggtgt ggatctacgt
     1201 tcagtggccg cccccggaaa ccgtggaagg cttctacgcc ctcttcgccg agcgcccctt
     1261 tcttgggctg ctgagcatgg acctgctcta catcgtcaac aacgcgattg tccttctcgt
     1321 ctacctggcc ttgttcgccg tgcttcgtca cgcctatccg tcgaccgtcg ccatcggtct
     1381 gctgttggga gcgatgggaa tggcggcata catggcctcg aataccggtt ttgagatgct
     1441 gtcattggcc aacgcctacg ccacggccga cgccaccggt cgggtcgcgc tcattggtgc
     1501 cggggaggcg atgctggctg tgttcgaggg cactgccttc gacatctatt acgtgctcag
     1561 cgcgatcgct ctattcctct tcgccggatc gatgttgcgt tcccgtctgt tcagccgtgc
     1621 cacgggcttc tggggattgg cggcggcggt gttgatgatc gttccctcca cggccgggac
     1681 ggtggggatg gtcttcgctc tcggatctct catcccctgg gtcgcctttt cagtgctcac
     1741 cggtcgccag ctcttgtacc tcggctcgac cgggcgaggc tcagaggccg ggctatgagg
     1801 ctcctgtcct acaccctcga agagatgatc gacaggtcac cggctgaggt attcgacttc
     1861 tgctccgatc tgagaaacga acttgtatgg aatccgacgt cggagtatgt ggagaagctc
     1921 actgacggac cggtcggggt gggtacgagg tatgcggcga aatggtccaa caccaggagg
     1981 gtgatcgtcg aggtttctgg cttcgatccc tcgacgacgt gggagacgcg ttcccgcgct
     2041 ggcgggctga cgatcaccgt ccaagggagg ctgtccgatc tggcccctgg aacaagatac
     2101 gagacgacag tgggagtgga ggccaggggg ctggcccatc tgtatgcgcc gc
//
LOCUS       DASUTA010000134         3339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_560661, whole genome shotgun sequence.
ACCESSION   DASUTA010000134 DASUTA010000000
VERSION     DASUTA010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560661"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VFZ80_02750"
     CDS             <1..1008
                     /locus_tag="VFZ80_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX6286377.1"
                     /translation="ITGILAQRWFGNIGVTIASIVLTIVLYIYGEAIPKTYAVRHAER
                     TAKFVTAPIAFMERVFRPVVSLLVWIADIQMPGKGVTTSATVTEDELRLLAVEAADEG
                     EITEIDRHLIDRVFRFGDREVDDIMVPRPDIVAVEASTPLSEALQTALQAGHRRLPVY
                     TDDMDNIDGVVRLRDLIEARDQGETTLTPLTYEPLVVPESKRVSGLLADMQEQNNHMA
                     VVVDEYGVTVGIVTIEDVAEELLGSISDEPARPDLRQVGPGQWDIAGSLPVEDLAAEL
                     GVPIPEGEWNTAAGLMLGVAGRLLRPGATVNVDGYEMKVETVRRRRITRISIRRLPDV
                     S"
     gene            complement(1003..1443)
                     /locus_tag="VFZ80_02755"
     CDS             complement(1003..1443)
                     /locus_tag="VFZ80_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6286378.1"
                     /translation="MPSQTFTHTALTDAPAPVVWASLDEPETWEAIGGVDRVFNPTID
                     GDGRLRGFSFDTVAAGKRYVGVATPHRREEGRLMAWHIQNSEVRGITTVELDTHESQT
                     AVSVTLEVESRGLLSGMFFPVIASAIGNGLPKAVDEFAAGLSIS"
     gene            1648..2058
                     /locus_tag="VFZ80_02760"
     CDS             1648..2058
                     /locus_tag="VFZ80_02760"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX6286379.1"
                     /translation="MSDASDRVASVFDRAPFISHLGVELIGVGDDWVEAALTVSDQLR
                     QQHGFAHAGVVTTLADHTAGAAASTAISDGQSVLTADFSIHLLRPASGDRLTCRGEIV
                     KAGRTLIVTQADVLAEGKLCARYTGTMAVVDRAI"
     gene            complement(2114..2422)
                     /locus_tag="VFZ80_02765"
     CDS             complement(2114..2422)
                     /locus_tag="VFZ80_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018528749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6286380.1"
                     /translation="MSDPQQVLAALADPTRRAVFERLNTKGPASASQLAAELPVSRQA
                     IAKHLTQLDSVGLVDRSQQGREVVFSARVSPLSDVADWLETVGSDWDRRLRRLRDSFD
                     "
     gene            complement(2419..2769)
                     /locus_tag="VFZ80_02770"
     CDS             complement(2419..2769)
                     /locus_tag="VFZ80_02770"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX6286381.1"
                     /translation="MDDLPEIDRSIELPATPDEVWEKIADGDLAEEWMGVRVEPRPGG
                     RVVVPGKDTIGTVEEVEWGRSITWSWREPDGDPSQVIIEVEPTDAGSRVTITERLLEY
                     RITGIPPILLARAA"
     gene            2837..>3339
                     /locus_tag="VFZ80_02775"
     CDS             2837..>3339
                     /locus_tag="VFZ80_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX6286382.1"
                     /translation="MLIPTVIEQTSRGERSFDIYSRLLGHRVVFLGRPIDDDVANLIV
                     AQLLHLESDDPDKPIALYINSPGGSMDSMFAIYDSMQHIRPEVHTTCIGMAASAASLI
                     LAGGEAGHRSILPHGRVMIHQPWVQNVGGQASDIEIHAREIMRQRELANDILARHTGQ
                     DIDKIRE"
BASE COUNT          628 a         1041 c         1037 g          633 t
ORIGIN      
        1 atcaccggga tcctggcgca gcggtggttt gggaacatcg gagtgacgat cgcttcgatc
       61 gttctcacga tcgtcctcta tatctacggg gaggcgatcc ccaagaccta cgcagtccgt
      121 catgccgaac ggacagccaa gttcgtcacc gcacccatcg ccttcatgga gcgggtgttc
      181 cgcccagtcg tcagcctgtt ggtgtggatt gctgacatac aaatgccggg caaaggagtc
      241 acgaccagcg ccacggtcac agaggacgag ttgaggcttc tcgccgtcga agcggccgat
      301 gaaggggaga tcaccgaaat cgaccgtcat ctcatcgacc gggtgttccg gttcggcgac
      361 cgagaagtcg acgacatcat ggttccccgc cccgatatcg tcgccgtcga agcctccaca
      421 cctctgtcag aggcgctcca gaccgcgttg caggccggcc acaggcgtct accggtctac
      481 accgatgaca tggacaacat cgacggagtt gttcgactgc gcgacctgat cgaagcgaga
      541 gatcagggtg agacgaccct gactccgctg acctacgaac cgctggtcgt cccggagtcg
      601 aagagagttt ccggcctact cgccgacatg caggagcaga acaaccacat ggctgtcgtg
      661 gtcgacgagt acggggtcac cgtgggcata gtcaccatcg aggacgtcgc cgaggagctg
      721 ttgggcagca tctccgacga accggcccga cccgatctcc gtcaggtcgg tccggggcag
      781 tgggatatcg ctggatcgct gccggtcgaa gatctcgcag ctgagctcgg cgtgccgata
      841 ccagaagggg agtggaacac tgccgccggt ttgatgctgg gggtcgcagg tcgccttctc
      901 aggcccggcg ccaccgtcaa tgtggatggc tatgagatga aggtcgagac ggtgagaagg
      961 cggcgtatca cacgtatctc cattcggaga ctccccgacg tttcatgaga tcgatagtcc
     1021 cgccgcgaac tcgtcgacgg ctttgggtaa tccgttcccg atcgccgaag cgatgaccgg
     1081 gaagaacatc ccggagagga gcccgcgact ctcgacttcg agtgtgaccg agactgccgt
     1141 ctgactctca tgcgtgtcga gctcgacagt cgttatccct cgcacctctg agttctggat
     1201 gtgccaggcc atcaggcggc cttcttcgcg tcggtgtggc gttgccactc ctacatagcg
     1261 cttgccggcg gcaactgtgt cgaaggagaa tccgcggaga cggccgtcac cgtcgatcgt
     1321 tgggttgaaa actcggtcca ctcctccgat ggcctcccag gtctcgggtt cgtcgagcga
     1381 ggcccacacc accggcgcag gtgcgtcggt cagcgcggtg tgcgtgaaag tctgggaggg
     1441 cacggcggca acatagttgg ggaacgaatt cggggcgacg ttgccgtcgc cccgagtgcc
     1501 cctgggagga gggatctgtt gtcgttgcag gattccgtag gaacctgacg attcagttgt
     1561 aacgactcaa cgtgactgga gggttaacgc cgtgtgccaa cttggggaat gttccctgtg
     1621 cctggaggca tcgaataccg tcgtctcatg agcgatgcga gcgacagagt tgccagtgtc
     1681 ttcgaccggg ctcctttcat ctcccacctc ggggtcgagc taatcggggt cggcgacgac
     1741 tgggttgagg ccgccctcac cgtctccgac cagttgcgcc agcagcacgg ttttgctcac
     1801 gccggtgtcg tgacgactct ggccgaccac acggccggcg ccgccgcctc gaccgctatc
     1861 tcagacggac agtcggtcct gacggccgac ttcagcatcc atctgctccg ccccgcctca
     1921 ggggatcggc tcacctgccg aggtgagata gtgaaggcgg gcaggaccct cattgtgacc
     1981 caggcggatg tcctggccga aggcaagctc tgtgcccgct acaccgggac catggctgtt
     2041 gtcgaccggg cgatctgagc gtgtgcagat accggcgtca cagcacgttg tctgcacacg
     2101 ctccgctaac gggtcagtcg aatgagtctc tgagtcgcct caggcgccga tcccagtcgc
     2161 tgccaacggt ctccagccaa tcggctacgt cgctcaaagg gctgactcga gcggagaaca
     2221 cgacctcacg gccctgctgg gaccgatcga cgagtcctac cgagtcgagc tgggtgaggt
     2281 gcttggcgat tgcctggcgc gacacgggaa gctccgccgc gagctgtgag gcagaggcag
     2341 gccctttcgt gttgagacgc tcgaagacgg cgcgtcgggt cgggtcggcc agagcggcca
     2401 gaacctgctg agggtcgctc atgccgcccg ggcgagcagg atcggaggaa tgccggtgat
     2461 ccggtattcg agtagtcgtt cggtgatcgt cactcggctg ccggcgtcgg tgggctcgac
     2521 ctcgatgatg acctgggacg ggtctccgtc tggctcccgc cacgaccatg tgatcgatcg
     2581 gccccactcg acttcctcga cggtcccgat ggtgtccttg ccggggacca cgacacggcc
     2641 ccctgggcgc ggctctacac gcacacccat ccactcttca gccagatctc catcggcgat
     2701 cttttcccac acttcatcag gggtcgcggg caattcgatg cttctgtcga tctcgggaag
     2761 gtcgtccata cctgcaacct agcagttgca gagccatgtt aggctactgc aaccaaacgg
     2821 ttgcgaaagg accgccatgc tgatcccaac agtcatcgaa caaacgtcac gtggagaacg
     2881 ctcattcgac atctactcga gacttctcgg tcaccgagtg gtctttctgg gcaggccgat
     2941 cgacgacgac gtcgccaatc tgatcgttgc tcagttgctc catctggaat cggacgatcc
     3001 ggacaagccg atcgccctct acatcaactc tccaggtggc tcgatggact cgatgttcgc
     3061 catctatgac tcgatgcagc acatccgacc tgaggtccac accacctgca tagggatggc
     3121 ggcgtcggca gcatcgctca tcctcgccgg gggagaggcc ggccacagat cgatcctccc
     3181 tcatgggcgg gtgatgatcc accagccctg ggtccagaat gtcggtggtc aagcatcgga
     3241 catcgagatt catgcccggg agatcatgcg ccagcgcgag ctcgccaacg acatcctcgc
     3301 tcggcacacc ggccaggaca tcgacaagat ccgtgagga
//
LOCUS       DASUTA010000135         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1597781, whole genome shotgun sequence.
ACCESSION   DASUTA010000135 DASUTA010000000
VERSION     DASUTA010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597781"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..736)
                     /locus_tag="VFZ80_02780"
     CDS             complement(101..736)
                     /locus_tag="VFZ80_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6286383.1"
                     /translation="MSETQHARDLPSELDAIGDRLREERVKAGISQRELARRLGLSAS
                     LISQLESGLSKPSVGTLYAIVTELGLSLDRVIRGEDFDGGPRRVPASEVKSPLVHPGE
                     RQTIDLASGVRWEELTAETEDGVDFLHAIYEVGGASTPDESLMRHHGREYGYIISGQL
                     GLQIGFQSYELNPGDSIAFDSTEPHRLYNRGDEPVHAIWFVVGRAADSRTE"
     gene            complement(733..1425)
                     /locus_tag="VFZ80_02785"
     CDS             complement(733..1425)
                     /locus_tag="VFZ80_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004923036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6286384.1"
                     /translation="MADGAVLVVGGTAGIGREIAGHYAKQGRNVLITGRDDERCSAIA
                     AEIGESVESLAFDLARPETIDTALTDVGPVAHLVITAVARDHNPIREYNIATATDLIT
                     MKLVGYSETIHALIPKFTDDASVVLFGGLAKERPYPGGLTIATVNGGIATMINDLVVE
                     LAPIRFNAIHPGIVGDSPYWEGKDLQAVIDRTPTGRLTTMAEVVDATVFLLENRAING
                     VNLYIDGGWMRM"
     gene            1513..>2360
                     /gene="lhgO"
                     /locus_tag="VFZ80_02790"
     CDS             1513..>2360
                     /gene="lhgO"
                     /locus_tag="VFZ80_02790"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HEX6286385.1"
                     /translation="MSSEPRYDVCVVGAGLLGLATAHALTAMLPGVSILVLDKESQIA
                     AHQSSHNSGVVHSGLYYKPGSLKARLCVEGRDAVYEVCEAEGIPVERSGKLVIATEPG
                     EIPALDELERRGRANGLVGLTRLGPDGIKDHEPAATGIDALYVPEAGVADFPALAAHL
                     SERLRKAGAEVRLGREVTAIGHSIDGVDVTAGDDTYRTRSLVNCAGLHCDRVAALAGV
                     SSPVKIIPFRGEYYELVEGRRDLVRGLIYPVPDPRFPFLGVHFTRTVDGMIEAGPNAV
                     LALKREG"
BASE COUNT          401 a          752 c          757 g          450 t
ORIGIN      
        1 ggtgagagac ggtctgatca gctacgagaa cgcttggttc gacgccgccg acgtgcaacg
       61 tcaaatcgag gctttcaagc ctgagcgggg ggagtagggg ctactccgtg cggctgtcgg
      121 cggcccttcc gacgacgaac caaatggcat gcacgggctc gtccccgcgg ttgtagagac
      181 gatgcggctc tgtcgagtcg aacgcgatgg agtctccagg gttcaactcg taactctgaa
      241 agccgatctg gagcccgagc tggccgctga ttatgtagcc atactcccgt ccgtggtgtc
      301 gcatcagcga ctcgtcgggt gtcgacgctc cgcccacctc gtagatggcg tggagaaagt
      361 cgacaccatc ctcggtctcg gcggtcagct cctcccagcg cacgcccgag gcgaggtcga
      421 tggtctgccg ctcgcctgga tggacgaggg gcgacttgac ctcgctcgcc ggcacccgtc
      481 tcgggccacc gtcgaagtcc tcgccacgga tcacccgatc gagtgacagc ccgagctcgg
      541 tgacgatcgc gtagagggtt cccaccgatg gcttggagag gccactctcc aattgagaga
      601 tgagactggc cgacaggcca agtcggcgtg ccagctcccg ctggcttatc cccgctttca
      661 cccgctcctc gcggagtcgg tcgccgatgg cgtcgagctc gctgggcagg tctcgggcat
      721 gctgggtttc gctcacatcc gcatccagcc accgtcgatg tagagattca cgccgttgat
      781 cgcgcggttc tccaacaaga acaccgtagc gtccaccacc tctgccatcg tcgtcagacg
      841 acccgtgggg gttcgatcga tcaccgcttg gagatccttg ccttcccaat acgggctgtc
      901 tcccacgatc cccgggtgga ttgcgttgaa cctgattgga gcgagctcga ccaccagatc
      961 gttgatcatc gttgcgatgc ccccattcac cgtggcgatg gtgagacccc ccggataagg
     1021 ccgttccttg gcgagaccgc caaagagcac caccgaggcg tcgtcggtga acttcggtat
     1081 cagagcatgg atcgtctccg agtaaccgac cagcttcatc gtgatcagat cggtggccgt
     1141 cgcgatgttg tactccctga tcgggttgtg gtcgcgtgcc accgcggtga tgacgagatg
     1201 agcgaccgga ccaacatcgg tgagagccgt gtcgatcgtc tcgggtctgg cgagatcgaa
     1261 tgcgaggctc tccacacttt ccccgatctc cgctgcgatc gcagagcatc gctcgtcgtc
     1321 gcgaccggtg atgagcacgt tgcggccctg tttcgcgtag tgcccggcga tctcccgtcc
     1381 gatacccgcg gtgcccccta ctacgaggac ggcgccgtct gccatttcga ctagctcctt
     1441 ggtcgtggat gggcgaggat atacggatga acgggagccg tgcagtgacg ctgaacactt
     1501 tcatcattca ccgtgagctc tgagcctcga tacgacgtct gcgtcgttgg agccgggctc
     1561 ctcgggctgg ctacggccca cgcactcacc gcgatgctgc ccggggtgtc catactcgtt
     1621 ctcgacaaag agagccagat tgcggcccac cagagcagcc acaacagcgg cgtggtccat
     1681 tccggtctct actacaagcc agggtcgctc aaggcccggc tttgtgtcga aggtcgcgat
     1741 gcggtgtacg aggtttgtga ggcggagggt atcccggtcg agcgatcggg caagctggtc
     1801 atcgccacag agccgggtga gattccggcc ctcgacgaat tggagcggcg gggtagagcg
     1861 aatggcctgg tcggcttgac gaggctcggc ccggacggga tcaaggacca cgagccggcg
     1921 gcgactggca tcgacgccct gtacgtccct gaggccggtg tcgccgactt ccctgccctg
     1981 gcggcccatc tgtccgagcg actgcgcaag gcaggcgccg aagtgcggct gggtcgtgag
     2041 gtcacggcca tcggccactc catcgacggg gtggatgtga ccgcaggtga cgacacctat
     2101 cgaacgcgat ctctcgtcaa ttgcgctggg cttcattgcg atcgagtggc tgctctggcc
     2161 ggtgtgagct caccggtgaa gatcatccct ttccgcggcg agtactacga gctggtggaa
     2221 ggccggcggg acctggtgcg cggactgatc tacccggtgc ccgacccgcg cttccccttc
     2281 ctgggcgtcc acttcacgcg cacggtggac ggcatgatcg aggccggccc caacgccgtc
     2341 ctcgccctca agcgcgaagg
//
LOCUS       DASUTA010000136         2696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_561604, whole genome shotgun sequence.
ACCESSION   DASUTA010000136 DASUTA010000000
VERSION     DASUTA010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561604"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /gene="queC"
                     /locus_tag="VFZ80_02795"
     CDS             complement(<1..518)
                     /gene="queC"
                     /locus_tag="VFZ80_02795"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX6286386.1"
                     /translation="MNQPTRAVVLVSGGLDSTTLLAMARDQGREVYALSFDYGQRHRH
                     ELHAARRVAEAFGAIEQKVALIDLRAFGGSALTSEMEVPKDAVGDQNIPVTYVPARNM
                     IFLAFAVGYAEVVEADEIWIGVNAVDYSGYPDCRPEFIRAFQEAVRTGTRSGVESGRP
                     RIVTPLIDMTKGG"
     gene            complement(921..1727)
                     /locus_tag="VFZ80_02800"
     CDS             complement(921..1727)
                     /locus_tag="VFZ80_02800"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX6286387.1"
                     /translation="MLKRHNLVTPRRTRKRGKTPFARTYQAMEPGDLTTIDFKGEFLL
                     GNGKYCYPLTIMDYSSRFLLDCRGLGSTALSGVWETLCRVFREHGLPKALMSDNGVPF
                     AAPNHARLSTMSVRLMQLGIQPVFTRPGHPQDNGAHERMHLDLKRSTTRPPEANMHNQ
                     QERFDRFRQIFNEERPHEALELDRPAHRHRRSPRSMPRKIPHTDYPLHFAKRLVSSSG
                     TFKWRNETIFIGQPLAGQYIGLEPVDEKYLNIYYYDFLIGKLDEDNGEVI"
     gene            2173..>2696
                     /locus_tag="VFZ80_02805"
     CDS             2173..>2696
                     /locus_tag="VFZ80_02805"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6286388.1"
                     /translation="MIFDVHIHVQPWQMMKPSVLALMDDEAHQGVKAILESPEALLRE
                     LDANGVDRACCINYVSPDVMGFTFGVNPWVAEFTRKHRDRLIPVGSVNPLHSGDVRGD
                     ARRVIDLGIRMVKIHPPHQLFSPNAYRFELPKLAELYEECEKAAIPVMFHTGTSIFPG
                     ARNVYADPMPIDDVA"
BASE COUNT          534 a          795 c          787 g          580 t
ORIGIN      
        1 ccgcccttgg tcatgtcgat gagtggcgtc acgatccggg gcctgccgct ctcgactccc
       61 gacctcgtcc cggtacggac cgcctcctgg aatgcacgga tgaactccgg ccggcaatcg
      121 ggatagccgc tgtagtcgac cgcgttcacg ccgatccaga tctcgtccgc ctcgaccact
      181 tcggcgtacc ccacggcgaa tgcgagaaag atcatgttcc gcgccggcac gtaggtgacc
      241 ggaatgttct ggtcccccac agcgtctttc ggcacctcca tctcggaggt cagcgcgctc
      301 ccaccaaatg cacgaaggtc aatgagcgcg accttttgct cgatcgcgcc gaacgcttcg
      361 gcaacccgac gcgcagcatg cagctcgtga cggtgccgct gtccatagtc gaacgacagg
      421 gcgtagacct ccctcccctg gtccctggcc atggcgagaa gcgtcgtcga gtcgagcccc
      481 cccgaaacga ggacaacggc tctggtgggc tggttcatgg gcgagagtct ataggagtga
      541 gtgagccgtg ggcggggttc aagggggttg tcgggttctc ggttctcagt tctcggttct
      601 cggttctcag ctctcggttc tcggctttca gtagacggag tggactcagt ggacagaagc
      661 gtggacaaag gagtggacga cagagtggac ggtggggcat tctgcctggt ttctacgtct
      721 gtccactcct tcgtccacgc ttccgtccac gtttctgtcc accaagtcca gaaccgagaa
      781 ccgagaactg ggcgcaacca ggatgatcct ttccactcat gtctaggttg atcctttcca
      841 ctcgcagcgg cggcagtagc tgtgggaaag tgggaaacac ggtggttggg tgggtggtgt
      901 tttccacttt tccatcagct tcagatgacc tctccattgt cctcgtcgag ctttccgatg
      961 aggaagtcgt agtagtagat gttcaggtac ttttcatcga ccggttcgag accgatgtac
     1021 tgccccgcga ggggctgacc gatgaagatc gtttcgtttc tccacttgaa cgtcccgctc
     1081 gagctgacca gtcgctttgc gaagtgcaag gggtagtcgg tgtgcggaat cttccgaggc
     1141 atcgagcggg gcgagcgacg atgtcgatga gctgggcggt cgagctcaag cgcctcgtga
     1201 gggcgctctt cgttgaagat ctgtcggaac cggtcgaatc gctcttgctg attgtgcatg
     1261 ttggcttcgg ggggccgagt cgtcgacctc ttcaaatcaa gatgcatccg ttcgtgagcc
     1321 ccgttgtcct gaggatgccc cggacgagtg aagaccggct gaatccccaa ctgcatcagt
     1381 ctgacgctca tggtcgacag ccgggcatgg ttcggtgcag cgaacggaac cccgttgtca
     1441 ctcataagtg ctttaggcag accatgttcg cgaaagacac ggcagagtgt ctcccacact
     1501 ccggacagag cagtcgagcc caagcctcgg cagtccagaa gaaaccgact cgagtagtcc
     1561 atgatcgtca acgggtagca gtatttgccg ttgccgagaa gaaactcgcc tttgaagtcg
     1621 atggtggtca gatcacccgg ttccatcgcc tgataagtcc gggcgaacgg ggtcttccct
     1681 cgtttgcgcg tacgtcgggg agtcaccagg ttgtgacgct taagcatcgc gtcgactgcc
     1741 gatcgcgatg gaagccgaat atccgggttc tggtctctca aacggcggat gattttcttc
     1801 gaaccgaagc cccagcggat acgctcagcg ataatcaact cttcaatctc cgctggcgtt
     1861 ttgtgggggc acgtcttcgg agcacgagag cgctcgacca gaccagatcg cccgttctcc
     1921 cgatagcgct tccgatactc cctcaccgtc ggtcgagata catcaaaccg ctcagcaatc
     1981 tcagtcactg tcattttccc cgacaacgcc agcaatgcga tctcttcttt gatgtccatc
     2041 ttcgtcgtct cccgtacggg catccttcac ctccacgacg aagtggaaac aatctaccta
     2101 gacaactctg gcaacaatgt acctagaatg gaccctgaga actgagaacc gagaactgag
     2161 aaccaaaaga cgatgatctt cgacgttcac atccacgttc agccgtggca gatgatgaag
     2221 ccttcagtgc ttgcactgat ggacgatgag gctcatcagg gtgtcaaggc gatcctggag
     2281 tctcccgagg cgttgctgcg ggagctcgac gcaaacggag tggaccgggc gtgctgcatc
     2341 aactacgtct ctcccgacgt gatgggattc accttcggag tcaatccgtg ggtcgcggag
     2401 ttcacacgga agcaccgtga ccggctgatc ccggtcggat cggtcaatcc gctgcattcc
     2461 ggtgacgtca ggggggacgc cagacgtgtg atcgatctcg ggatacggat ggtgaagatt
     2521 catcctcccc atcaactctt ctcaccgaat gcctaccggt tcgagctccc gaaactcgcc
     2581 gagctctacg aggaatgcga gaaggccgcg atccccgtga tgttccacac ggggacctcg
     2641 atctttccgg gtgcgaggaa cgtgtacgcc gacccgatgc cgatcgacga cgtcgc
//
LOCUS       DASUTA010000137         3654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_173305, whole genome shotgun sequence.
ACCESSION   DASUTA010000137 DASUTA010000000
VERSION     DASUTA010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173305"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..412)
                     /locus_tag="VFZ80_02810"
     CDS             complement(137..412)
                     /locus_tag="VFZ80_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015795172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX6286389.1"
                     /translation="MGLVRGMARTAVVAGTATAVSNRVSRRQANRWADQEAAQYQEPQ
                     YAQAPPAAAAPSTDDKIAQLKELAELKDAGVLTEAEFEAQKAKVLAG"
     gene            complement(429..875)
                     /locus_tag="VFZ80_02815"
     CDS             complement(429..875)
                     /locus_tag="VFZ80_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003935457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HEX6286390.1"
                     /translation="MSDIDVEMGPVDYIVVEWPKGSPPDGQAFPHLVDLVDRGIIRLL
                     DLAFVQKDEEGNVIAIDLSDLDLDGDPELAVFVGVSSGLLDEEDYESAGEILEPGVAA
                     AILLYENSWAAPFATALRKAGARLVASGRIPINELISTLDELEQEA"
     gene            1099..1872
                     /locus_tag="VFZ80_02820"
     CDS             1099..1872
                     /locus_tag="VFZ80_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase"
                     /protein_id="HEX6286391.1"
                     /translation="MAKALDDAQLSGLLEEMGRADSVELKLTVPDSGMRSAVESLDLD
                     PLEARMRQVVFFDTPDLTLYNSGVVVRARRMQGGAGDTVVKLRPLPKGVKLGKGQRPK
                     GLSVEVDAMPGGFVCSGSIKGTTTAPDVRKVTRGQAKIGGLFSKTQKDFYRQHAPAGL
                     KMRDLSVLGPITLLKLKFNPKTLGRAMVAELWMYPDGSRILELSTKSVPGDAFQAAAE
                     ARVYLADHGIDLTAEQQTKTKTALKYFASRLSPSPEEAA"
     gene            complement(2029..2214)
                     /locus_tag="VFZ80_02825"
     CDS             complement(2029..2214)
                     /locus_tag="VFZ80_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286392.1"
                     /translation="MELIVGVGVATWVAHLYAEVLGNHVRSVDAFRPHALRRAMTTGY
                     RFGRRRCSLVSPCCWAA"
     gene            complement(2357..>3654)
                     /locus_tag="VFZ80_02830"
     CDS             complement(2357..>3654)
                     /locus_tag="VFZ80_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HEX6286393.1"
                     /translation="WVPTSKTEFPVSAAVAALVFDAWGLPRPETRGDVYEVARFLEEL
                     VAPHPDLEIVDVHKRRVRYSIEGCMAELTSLTANGRKTKTIAAETEDPKAVRSAVMRL
                     GMTGHVNMSYNRGLRCLLAMAPVRYAVVDIGTNSVKFHIGEQHPDGSWTRVVDRAEVS
                     RLGEGLDETGEIQPKPLERTVAAVATMVSEAEEGEVLATAAVGTAALRLASNSAWATD
                     TIEERVGIPVEVVSGEEESRLAFLAVSAGVGVVDGNVVVFDTGGGSTQFTFGRGGDVV
                     ERYSVDVGAVSNTERFGLDRAVDEHTIESARDALGEDLASLDGRDRPDSLVGMGGAMT
                     NITAVALSLQPYDPDVVQGATVTREEVERQIELYRSMSHEERREIVGLQANRAPVILA
                     GALIVATVMEKLGQESLVVSDRGLRHGLIRERFGLFSTS"
BASE COUNT          698 a         1230 c         1050 g          676 t
ORIGIN      
        1 gctcgcaggc gctcgatttc gtcgccttcg ggcacattct ctggagcgcg cgagtttttt
       61 gattccaccg gtagaactcc ctccgagaag gaggatcacg gattcacatc ttgctcgggt
      121 cgaaacccag ccggcgtcag ccggcgagaa ccttcgcctt ctgcgcctcg aactcggcct
      181 cggtgagaac gcctgcatcc ttcagctcag cgagctcttt gagctgggcg atcttgtcat
      241 ctgtagacgg tgcggcggct gccggcggcg cttgggcgta ttgcggttcc tgatactgag
      301 ctgcctcctg gtccgcccat cggtttgcct gccgtcgcga gacgcggttc gatacggcag
      361 tcgccgtgcc tgctaccacc gcggtgcgag ccattcctct gactaaaccc attgtgtgtt
      421 gcctcctatc aggcttcttg ctcgagctcg tcgagcgtag agatcagttc gttgatcgga
      481 atccggccgg aagccacgag gcgggcgccg gccttgcgca gagctgtggc gaacggtgcc
      541 gcccagctgt tctcgtagag aaggatcgcc gccgccacgc caggctcgag gatctctcca
      601 gcactctcat agtcctcttc gtccaacaga cccgaggaca ctccgacgaa gaccgccagt
      661 tcggggtcac cgtcgagatc caagtccgac aggtcgattg cgatcacgtt cccctcctcg
      721 tccttctgga cgaatgcgag gtcgagaagt cgaattatcc cgcgatcgac caggtcgacg
      781 aggtgcggga atgcttgtcc atcgggaggc gagcccttgg gccattccac gacgatgtaa
      841 tcgacgggtc ccatctccac atcgatgtcg ctcataagta tcccttctac taacgaagct
      901 tcggtggagc atggcacgcc gtgtcacggc agacatcccc ccaactgggc taaccggaat
      961 cacagatcac acgccccggg cgcgaatcgc cccgtttgga ggacgaatgc agaacggttc
     1021 cgtcgtacaa atagacgacg caacctccga tcgggccgtc tggcccctct tacgagcccg
     1081 cggagtctgg aggagtagat ggcgaaagca cttgacgatg cccaattgtc cgggctcctg
     1141 gaggagatgg gcagggccga ttcggtcgag ctgaaactga cagttcctga ctcgggcatg
     1201 agatcggccg tcgagtctct cgacctcgat ccactcgagg ctcggatgcg gcaggtcgtg
     1261 ttcttcgaca ccccggatct gaccctgtac aacagtggtg tcgtggtgcg agcgcgtcgc
     1321 atgcagggtg gggccggcga cacggtggtc aagcttcgtc cccttccgaa aggcgtgaag
     1381 ctgggcaagg gccaacgacc caagggcctg tcagtcgagg tagacgccat gcccggcggg
     1441 tttgtctgta gcggatcgat caaggggaca acgaccgccc ctgacgtgcg aaaggtcacc
     1501 cgaggacagg cgaagatcgg cggtctcttc tccaagacgc agaaagactt ctatcgccaa
     1561 cacgctcctg caggattgaa gatgcgcgat ctcagcgtgc tcggcccgat aaccctgttg
     1621 aagctcaagt tcaatcccaa gacactgggt agagccatgg tcgccgagct ctggatgtac
     1681 cccgacgggt cgagaatcct ggagctctcg acgaagtcag tgcccggcga cgccttccag
     1741 gctgcagccg aggcgcgcgt ctatctggcc gaccacggaa tcgatctgac cgccgagcag
     1801 cagaccaaga ccaagaccgc cttgaaatac ttcgcctcca ggctctcccc ctcccccgag
     1861 gaagcggcct aagagacatc tggcagccat cgatgaccga ccaagccctc ggcgtctcgg
     1921 gacctcccgg tgaatgaggt ggtcgcagcc ggcgctcctc gtccagacat ccctccagca
     1981 ccgccaggat caccgctatc caaagcgctt gcctcgctgc gacgatcttc aagcggccca
     2041 gcaacagggc gacaccagag agcaccgacg acggccgaat cggtagcccg tcgtcatcgc
     2101 cctgcgcaga gcatggggcc gaaaagcatc gacgctccgg acgtgattcc ccaggacttc
     2161 ggcatagagg tgggcgaccc aggtggcgac acccacgccg acgatgagct ccatcccaga
     2221 ccggaggcaa catcctccgt ctcgaccagg tgaagagcag cgatctatca ggactgtgcc
     2281 gtaggaggcg gctctgaccc gctcagccaa ccggtcacga tatgcactgc ttacagctcg
     2341 cgagagtggc ctgttctcag gaggtggaga acaacccgaa gcgctcccgt atcagaccgt
     2401 gacggagccc gcgatcgctg accactagag actcctggcc gagcttctcc atgaccgtgg
     2461 caacgatcaa ggccccggcg agaatcaccg gtgccctgtt ggcctgaaga ccgacgatct
     2521 cgcggcgctc ctcatgactc atcgatcgat agagctcgat ctgccgttcc acttcctccc
     2581 tggtgacggt ggctccctgc accacgtcgg ggtcgtaggg ctggagtgac agcgccaccg
     2641 cagtgatgtt ggtcatcgcc ccgcccattc ccacaaggga gtcaggcctg tctcgcccat
     2701 ccagactggc aaggtcttcg cccagagcgt ctcgagctga ttcgatggtg tgctcgtcaa
     2761 ccgctcggtc caggccaaag cgctccgtgt tcgacacagc tccgacatcg acactgtaac
     2821 gctcaaccac gtcgcccccg cgaccgaatg tgaactgtgt gctgccacca ccggtgtcga
     2881 agacgaccac attgccgtcc accaccccca ctccggcgct cactgccagg aaggcgaggc
     2941 gactctcttc ctcacccgac accacctcaa ccggaattcc gacccgctcc tcgatggtgt
     3001 ctgtggccca agccgaattc gacgcgagcc tgagggccgc ggttcccacc gcagctgtcg
     3061 caagcacttc cccttcctcg gcctcggaca ccatcgtcgc aaccgccgcg acggtccgtt
     3121 ccaggggctt tggttgaatc tcgcccgttt cgtcgaggcc ttcacccagc cggctcactt
     3181 cggcccggtc caccaccctc gtccatgagc cgtctgggtg ctgttctccg atgtggaact
     3241 tcaccgaatt ggtcccgatg tcgacgaccg cgtacctgac cggcgccatt gcgagcagac
     3301 agcgcaagcc tcggttgtag ctcatgttga catgcccggt catacccagt ctcatcacgg
     3361 ccgacctcac cgccttcgga tcctccgtct ccgcagcaat cgttttcgtc ttccgtccgt
     3421 tggcagtcaa cgaggtcagc tcggccatac agccctcgat cgagtatcga accctgcgct
     3481 tgtggacatc cacgatctcg aggtccggat gcggcgcgac caactcttcg agaaaccggg
     3541 ctacctcata gacatctcca cgagtctccg gtcggggcag accccaggca tcgaacacca
     3601 gcgcggccac cgcggcggag actgggaact ccgttttcga ggtcggaacc caca
//
LOCUS       DASUTA010000138         2085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_690550, whole genome shotgun sequence.
ACCESSION   DASUTA010000138 DASUTA010000000
VERSION     DASUTA010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690550"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VFZ80_02835"
     CDS             <1..497
                     /locus_tag="VFZ80_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6286394.1"
                     /translation="TGELYQPEDLSVIDYNEIETAMLQDAVWVDADWIAEEGNEDIAI
                     RFLTASFRGWIHCLDNFDECVEIVLDNGSTLGQSHQEWQLNEILALIFPATNGIGVMN
                     DELWDQTVAIATEQITELQGVEIPDDVYRTDLAQAAIDRLGDADVEGSGFERREIELR
                     EGGN"
     gene            587..1429
                     /locus_tag="VFZ80_02840"
     CDS             587..1429
                     /locus_tag="VFZ80_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX6286395.1"
                     /translation="MANIVRAAIVQTEWTGDKDTMIKKNADYAREAAKQGAKVMCFQE
                     LFYGPYFCQVQENEHFDYAEPIPDGPTTRLMQDLAKETGMVLVVPIFEMEQEGFYYNT
                     ASVIDADGTYLGKYRKNHIPHVKGFWEKFYFRPGNMGYPVFDTAVGKVGVYICYDRHF
                     PEGWRALGLNGAKIVFNPSATSRGLSMYLWNLEQPAAAVANEYFIGAINRVGQEPFGD
                     NDFYGSSYFVDPRGQIIDGSASDTEEELVIRDLDLDLIEEVRQQWAFYRDRRPDAYGD
                     LVAP"
     gene            1440..>2085
                     /locus_tag="VFZ80_02845"
     CDS             1440..>2085
                     /locus_tag="VFZ80_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018519902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6286396.1"
                     /translation="MARTVIEGGTVVTATEQTQADVLIEGERVAAIAAAGSHDWGAGA
                     DKVIDAAGKYVIPGGIDVHTHMELPFGGTFAADTFETGTRAAAWGGTTTIIDFAVQTK
                     GQNLMDGFEAWMGKADGNCAIDYGFHLIVGDVTDQSLKEMDLALEEGVTSFKMFMAYP
                     GVFYSTDGEIFRAMQKASENGSTIMMHAENGIAIDVLVEQALSRGATDPIYHGLT"
BASE COUNT          429 a          641 c          674 g          341 t
ORIGIN      
        1 agaccggtga gctgtaccag ccggaggacc tgagcgtcat cgactacaac gagatcgaga
       61 ccgccatgct ccaggacgca gtgtgggtgg acgccgactg gatcgccgaa gaaggaaacg
      121 aggacatcgc catccgtttt ctcaccgcca gcttccgtgg ctggatccat tgtctcgaca
      181 acttcgacga gtgcgtcgag atagtgctcg acaacgggtc aacgctcggt cagagccatc
      241 aggagtggca gctcaacgag atcctggctc tcatcttccc cgccaccaac gggatcggcg
      301 tgatgaacga cgagctgtgg gaccagacgg tggccatcgc caccgagcag atcaccgagc
      361 tgcagggggt ggagatcccc gatgacgtct accgcaccga cctcgcccaa gccgcaatcg
      421 accgtctcgg cgacgccgac gtcgaaggct ccggcttcga acggcgcgag atcgagcttc
      481 gagaaggtgg caactagcga tcggtccccc cgctttgcgg ggggacctgt cggtctttca
      541 tcacagaggg gggccgacag ctaggtttgg cgcaaaggag gactcaatgg ccaacatcgt
      601 cagggccgcc atcgtccaga ccgagtggac gggggacaag gacaccatga tcaagaagaa
      661 cgccgactac gcccgcgagg cggcgaagca gggggcgaag gtcatgtgtt tccaggagct
      721 cttctacggt ccctacttct gccaagtcca ggagaacgag cacttcgact acgccgagcc
      781 gatccccgac gggcccacca ccaggctgat gcaggacctg gccaaggaga ccgggatggt
      841 gctcgtggtc cccatcttcg agatggagca ggagggtttc tactacaaca ccgcctccgt
      901 gatcgacgcc gacggcacct acctcggcaa gtaccgcaag aatcacatcc cccatgtgaa
      961 gggcttctgg gagaagttct actttcgccc cgggaacatg gggtatcccg tgttcgacac
     1021 cgcggtgggc aaggtcgggg tgtacatctg ctacgaccgc cacttccccg agggctggag
     1081 ggcactgggc ctgaacgggg ccaagatcgt gttcaaccct tcggccacca gccgggggct
     1141 gtccatgtac ctgtggaacc tggagcagcc ggcggcggcg gtagcgaacg agtacttcat
     1201 cggcgccatc aaccgagtgg gccaggagcc gttcggagac aacgacttct acggctcgtc
     1261 gtacttcgtc gatccgaggg gccagatcat cgacggctcc gcctccgaca ccgaagagga
     1321 gttggtgatc cgggacctcg atctcgacct gatcgaagag gtgcgtcagc agtgggcctt
     1381 ctatcgggat cgccggccgg acgcctatgg cgacctggta gcaccctgag gaggatgaaa
     1441 tggcacggac cgtcatcgag ggaggaacgg tcgtcaccgc gaccgagcag acccaggccg
     1501 acgttctgat cgaaggggag agggtcgccg ccatcgcagc ggcggggagc cacgattggg
     1561 gggcaggggc tgacaaggtg atcgatgcag ccggcaagta cgtcataccc ggcggtatcg
     1621 atgtccacac ccacatggag cttcccttcg gggggacctt cgccgccgac accttcgaga
     1681 ccgggacccg ggccgccgcc tggggcggga ccaccaccat catcgacttc gccgtgcaga
     1741 ccaagggcca gaacctgatg gacgggttcg aggcgtggat gggcaaggcc gacgggaact
     1801 gcgccatcga ttatgggttc catctgatcg tcggcgacgt cacggaccag tccctgaagg
     1861 agatggacct cgctctcgag gagggtgtga cctccttcaa gatgttcatg gcctaccccg
     1921 gtgtcttcta ctccacagac ggggagatct ttcgggcgat gcagaaggcg tcagagaacg
     1981 gctcgacgat catgatgcac gccgagaacg ggatcgccat cgacgtcctg gtcgagcagg
     2041 cattgtcccg tggcgcgacc gatcccatct atcacggtct caccc
//
LOCUS       DASUTA010000139         3310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1468094, whole genome shotgun sequence.
ACCESSION   DASUTA010000139 DASUTA010000000
VERSION     DASUTA010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468094"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /gene="glgB"
                     /locus_tag="VFZ80_02850"
     CDS             <1..1013
                     /gene="glgB"
                     /locus_tag="VFZ80_02850"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HEX6286397.1"
                     /translation="HIDGLRVDAVASMLYLDYSRKQGEWVPNIHGGRENLEAIAFLRR
                     LNETVYLEYPDVQTIAEESTAWPMVSRPTFLGGLGFGMKWDMGWMHDTLHYMANDPVH
                     RSYHHHELTFRAVYAFTENFMLPLSHDEVVHGKGSLLGKMPGDEWQQLANLRVLLCQM
                     WTQPGKKLLFMGGELAQPSEWAHDGQLDWGIGQSPGHAGVSRLLGDLNRLYRDYPALH
                     QTDMRPEGFEWAVVNDSENSVLAYFRRMGDEVLLIVHNLTPVPRHEYRIGVPEPGRWV
                     EILNSDAEIYGGSGQGNLGGVDTGPLSVPSRGRPESIDVTLPPLATVVLKQTPRPSDE
                     EE"
     gene            1063..2589
                     /locus_tag="VFZ80_02855"
     CDS             1063..2589
                     /locus_tag="VFZ80_02855"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HEX6286398.1"
                     /translation="MSQSSYVCIHGHWYQPPRENPFTGVVGHQPTAAPFDNWNERITA
                     ECYEPNTHARILGEDGSVRREINNYEYVSSDWGPTLLAWLEDHHPETYGAIVAADRAS
                     MTRFDGHGTAMAQAYNHTILPLSNRRDKRTQILWGKADFIHRFGHDPEGMWLPETAVD
                     LESLELMADAGIAFTVLSPYQAAAVLEEGTWIEVVGGSIDTTVPYEVQLFGGRSIGVF
                     FYNGPLSQEIAFNGLLEDGALLAKRLVQALGEPDGRARLSHVATDGETYGHHHRHGEM
                     ALARAIEELESDHEVELTTYGRFLAEHPPTRIARIIEGTSWSCAHGVERWRADCGCAT
                     GQHPDWTQEWRTILREALDHLRDQLIEDFASLGATVLHDPWDARDHYIEVILGRSADA
                     FLEEHGNPGGGAEQRHLALDLLEIQHRAMLMYTSCGWFFDDIGGLEAVFVLRQAGRAI
                     ALAREAVGKDLEPGFLEILDHAQSNLDGRSGRDVYMDSVDPHMPEASGPVAGAEQTEH
                     "
     gene            2601..>3310
                     /locus_tag="VFZ80_02860"
     CDS             2601..>3310
                     /locus_tag="VFZ80_02860"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HEX6286399.1"
                     /translation="MADFRVTDKRVVIETVSPEVDNGRFPAKSPVGDPVVVEATVFAD
                     GHDVIRSLLRYQRAGSGRWAEVSMEPLGNDRWRGSFQPDELGLWQFEIEGWVDSFMTW
                     LDGLNKKLEAGVDVSVEVEMGARLYSEAADRARGDHSKRLTEIAAALVDGSPLATRIA
                     LATDEESVRLGEAYPDRSRATRSNTRWPVVVDREKATFSSWYELFPRSWSKKDGEHGT
                     FSDVEDRLDYVAGMGFDVL"
BASE COUNT          607 a         1044 c         1078 g          581 t
ORIGIN      
        1 accacatcga cggtctccgt gtcgatgctg ttgcctcgat gctctatctc gactactcgc
       61 ggaagcaggg cgagtgggtc cccaacatcc atggcggccg agagaacctc gaggcgatcg
      121 ctttcctgcg acgtctcaac gaaaccgtct acctcgagta tcccgatgtc cagaccatcg
      181 ccgaggagtc cacggcctgg cccatggtct cgagacccac ctttctcggt ggtctcgggt
      241 tcgggatgaa atgggacatg gggtggatgc atgacacgct ccattacatg gcaaacgacc
      301 ctgtccacag gagctatcac caccacgagc tcactttccg tgccgtgtac gccttcaccg
      361 agaacttcat gctgcccctg tcgcacgacg aggtggtgca cggaaagggt tcgctcctcg
      421 gcaagatgcc tggcgacgag tggcagcagc tggcgaacct gcgggttctc ctctgtcaga
      481 tgtggaccca gcctggcaag aagctcctct tcatgggtgg cgagctggcc cagccatcgg
      541 agtgggccca cgatggacag ctggactggg gcatcggcca gagccccggg cacgccggtg
      601 tctcgaggtt gttgggcgat ctcaatcgcc tctatcggga ttacccggct cttcaccaga
      661 ccgacatgag gcccgaaggc ttcgaatggg cggtggtgaa cgactccgag aactcggttc
      721 tcgcctactt ccggcgaatg ggtgacgaag tgctgctgat cgttcacaac ctgactccgg
      781 tgcccaggca tgagtaccgg atcggcgtgc ccgagcccgg tcgctgggtg gagatactca
      841 acagcgacgc cgagatatac ggggggtccg ggcagggcaa cctcggaggg gtcgacacgg
      901 ggccgctatc ggtgccgagt aggggccgtc cggagtccat cgacgtgacg ctcccgcccc
      961 tggcaaccgt cgtcctgaaa cagactcctc gcccgagcga cgaagaggag tgaacgagag
     1021 gtcccgctct accgggcaat cgccgctaat ttgactgccg cattgagcca gagcagctac
     1081 gtctgcatcc acggtcactg gtatcagcct ccccgggaga accctttcac cggcgtcgtc
     1141 ggccaccagc caaccgccgc gcccttcgac aactggaacg agcggatcac ggccgagtgt
     1201 tacgagccca acactcatgc ccggatcttg ggagaggatg ggtcggtccg gagggagatc
     1261 aacaactacg agtacgtcag ctccgactgg ggcccgacac tcctcgcctg gctggaggac
     1321 caccaccccg agacctacgg ggcgatcgtc gcggccgatc gcgcatcgat gacccgtttc
     1381 gacgggcacg gaacggccat ggcacaggcc tacaaccaca cgatcctccc gctgtccaac
     1441 cggcgcgaca agcgaaccca gatcttgtgg ggcaaagccg acttcatcca tcgcttcggt
     1501 catgacccgg agggcatgtg gctgccggag acggcggtag acctggagag cctggagctc
     1561 atggccgatg ccggcatcgc tttcacggtc ctgtcgccct accaggcggc tgcggtcctc
     1621 gaggaaggga cctggatcga ggtggtcggt ggctcgatcg acaccacagt gccttacgaa
     1681 gtccagctgt tcggtggtcg cagcatcgga gtcttcttct acaacggacc actctcccaa
     1741 gagatcgcct tcaacggctt gctcgaagac ggggcgctgc tggcgaagag attggttcag
     1801 gcgttgggtg agccggacgg gcgggcccgt ctctctcatg tggcaaccga cggcgagaca
     1861 tacggtcacc accaccgtca cggtgagatg gcgctggcac gcgcgatcga ggagctggag
     1921 tccgatcacg aagtcgagtt gaccacatac ggccgatttc tcgccgagca tcccccgacg
     1981 aggatcgccc gaatcatcga aggcacttca tggagctgcg ctcacggggt cgagcggtgg
     2041 agagcagact gcggctgcgc cacgggtcag cacccggact ggacccagga atggcggacc
     2101 atcctccgcg aggcgctcga ccacctgcgg gaccagctca tcgaggattt cgcgtcgctt
     2161 ggggcgacgg tgctgcacga tccgtgggat gcccgcgacc actacatcga ggtcatcctc
     2221 ggccggtccg ccgatgcgtt cctcgaggag cacggaaatc ccgggggtgg tgccgagcag
     2281 cgacatctcg ccctcgatct tctggagatc cagcaccgcg ccatgctcat gtacacgtcg
     2341 tgcggttggt tcttcgacga catcggaggt ctcgaagcgg ttttcgtcct ccgccaggca
     2401 gggagagcga tcgccctggc acgagaggcc gtcgggaagg acctagaacc gggtttcctc
     2461 gagatcctcg accatgcgca atcgaacctg gatggtcgca gcgggcgcga cgtctacatg
     2521 gattcggtcg atccccacat gccggaggcc tccgggccgg tcgctggcgc tgagcagaca
     2581 gagcactaga ttcggcctct atggccgatt tccgggtgac cgacaagaga gtggtcatcg
     2641 agaccgtgag cccggaggtg gacaatgggc gattccccgc gaagagccct gtcggcgacc
     2701 cggtcgtcgt cgaggcgacg gttttcgctg acggccatga tgtcatccgg agtctcctgc
     2761 gctaccagcg cgccggctcg ggccgctggg ccgaggtttc gatggagccg ttgggcaacg
     2821 atcgctggcg tggatcgttt caaccggacg agctggggct gtggcagttc gagatcgagg
     2881 gctgggtaga cagcttcatg acgtggctcg atggcctgaa caagaagctc gaggccggcg
     2941 tcgacgtctc ggtcgaggtc gagatgggcg cccggctcta ctccgaggct gcggaccggg
     3001 cccgcggcga ccactcgaag cgtctcaccg agatcgcggc cgcgctcgtt gacgggtcgc
     3061 cgctcgcgac tcgcatcgcc ctggcgacag acgaggagtc agtgcgtctt ggggaggcct
     3121 atcccgatag atcccgcgcc accagatcca acaccaggtg gcccgtggtc gtcgatcggg
     3181 agaaggcgac gttctcgtcg tggtacgagc tgtttcctcg ctcgtggtcg aagaaggacg
     3241 gagagcacgg caccttttcc gacgtcgaag accgtctcga ctacgtcgcc gggatgggct
     3301 tcgacgtcct
//
LOCUS       DASUTA010000140         2585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_562394, whole genome shotgun sequence.
ACCESSION   DASUTA010000140 DASUTA010000000
VERSION     DASUTA010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562394"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFZ80_02865"
     CDS             complement(<1..204)
                     /locus_tag="VFZ80_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286400.1"
                     /translation="MRGAYQKHGRWVLAFALATSHLFVTGGAVHAGIENYLDRFDTRS
                     YSNSDGSLVWTGPWEEVGETTDPL"
     gene            complement(222..1637)
                     /locus_tag="VFZ80_02870"
     CDS             complement(222..1637)
                     /locus_tag="VFZ80_02870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6286401.1"
                     /translation="MASQGLSSGLRLDRALSFSLAAGVALLLIVGVIYATANGTQRIT
                     SDAVSLHSADEALRSSSVARAQIAIAVHASTVDQQFGSDSSEAIEYALGEADRSLEDF
                     GSALRSLTEEGATSPELLTVAGDFVDTGRSIVAALATGRVDTARQLMSETLADRFAEL
                     VDGLVDRRESLADAVASADTRLGRMGDAAGFLVAFLLPVAVILTHRILARRHARRSAL
                     ESRLEAERRVSIAREEFIASASHELRTPLTSITGLAMLLEEHPAIAEDESSHELLQLI
                     VSESLDLARMVDDLLTSARLDVGALSYSFEDVEVSSELREITESMHRVGMTVSVDCEP
                     AHIRADRVRLRQVFRNLLSNASRYGGTSIRVEGRKDHKTYVCSVIDDGEGVSSQVAEQ
                     LFQKFTHKGRDTAQPGSVGLGLSIVQALTQGMGGSVRYERIDGETHFIVRLPLTDWAE
                     SQPEDTMAPSEDAPALVAGRA"
     gene            1839..>2585
                     /gene="recA"
                     /locus_tag="VFZ80_02875"
     CDS             1839..>2585
                     /gene="recA"
                     /locus_tag="VFZ80_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX6286402.1"
                     /translation="MSQEGRNVEREKALDMAVSQIERQFGKGAIMKMGEAANRKVDVI
                     PTGALSLDLALGVGGVPRGRIVELYGPESSGKTSLALHIVAEAQRNGGIAAFIDAEHA
                     LDPIYAKAIGVDVDELLISQPDTGEQALEIADMLIRSGALDVLVIDSVAALVPRAEIE
                     GEMGDTHVGLQARLMSQALRKLAGTLNKSRTTAVFINQLREKIGVMFGSPETTPGGRA
                     LKFYSSVRLDIRRIESIKDGTDSVGNRVKVK"
BASE COUNT          472 a          812 c          832 g          469 t
ORIGIN      
        1 cagggggtcg gtggtttccc cgacttcctc ccagggcccc gtccacacga gtgaaccgtc
       61 actgttggag taggagcggg tgtcgaacct gtcgaggtag ttctcgatac cggcgtggac
      121 ggctccaccg gtcacgaata ggtgtgatgt tgccagggcg aatgcgagga cccaccgtcc
      181 atgcttctgg tacgccccgc gcattcgatc cggccccgcc tctaagcccg ccctgcgacc
      241 agggccggag cgtcctcgga aggtgccatg gtgtcctccg gttggctttc ggcccagtcg
      301 gtgagtggca gtcggacgat gaagtgggtc tcgccgtcga ttctctcgta tcgcacactt
      361 ccgcccatcc cttgggtcaa cgcctggacg atcgatagac cgagcccgac gctgcccggt
      421 tgcgccgtgt cccgaccttt gtgggtgaac ttctggaaca actgctcggc gacctggctc
      481 gacaccccct cgccgtcgtc gatgaccgag caaacgtacg tcttgtggtc cttgcggccc
      541 tccacgcgga tggaggttcc gccgtatcgg cttgcattgg agagcagatt gcggaagacc
      601 tggcgcagtc gcacccggtc tgctctgatg tgggcaggct cacaatcgac cgagacggtc
      661 atgccgactc gatgcatcga ctctgtgatc tccctcagct ccgaggagac ttcgacgtcc
      721 tcgaatgaat aggacagggc gccaacgtcg agtcgcgccg aggtcaagag gtcgtcgacc
      781 atccgagcca gatccagaga ctcgctgacg atcagttgca gcagctcgtg ggaggattcg
      841 tcctcggcta tcgccggatg ctcctcgagg agcatcgcca gaccggtgat gctggtgagc
      901 ggggtgcgga gctcatggga ggccgatgct atgaactcct cccttgcgat cgagactcgg
      961 cgctcggctt ccagccggct ctccaaggcc gaccggcggg catggcgcct tgccaggatg
     1021 cggtgcgtca agatcacggc gacgggcagc aggaaggcga ccaggaaccc ggcggcgtcg
     1081 cccatccgac ccagcctcgt gtcggctgac gccactgcgt cggccagcga ttcccttcga
     1141 tcgaccagcc cgtcgaccaa ttcggcgaat cgatcggcga gtgtctccga catgagttgc
     1201 cgagcggtgt cgacccgacc ggtggcgagg gcggcgacga tgcttcgacc ggtgtcgacg
     1261 aagtcgccgg ccacggtcag caactccgga ctggtggcgc cttcctcggt gagcgaccgc
     1321 agggcagagc cgaagtcttc gagcgaccgg tcggcctcgc cgagggcgta ttcgattgcc
     1381 tcgctggagt cgcttccaaa ttgctggtcg accgtcgagg cgtgcactgc gatcgcgatc
     1441 tgggccctgg cgaccgagga ggatctgaga gcctcgtcgg cgctgtggag cgagactgcg
     1501 tcgctggtta ttcgttgtgt cccatttgcg gtggcgtaga tgacaccgac gatgagaagc
     1561 agagcgacac cggcggcgag ggagaacgag agcgctcgat ccaaccgcaa tccggacgac
     1621 aggccctggc tcgccatatg tttgtgtcgt caaatggggt gtgattcatc gagaaccatt
     1681 atggatagcc ccattcgcgc tagtccctgg ttttgggcgg caaacccctt gactcggcgg
     1741 atctggatgg gtaaatcaac agtgcgaaca catgttcggt ctaacgtgtc agcgatggat
     1801 cagatcttgt cacaggtcac cgatacggtc tcgcagagat gagtcaggaa ggacgcaacg
     1861 tggaacgcga aaaagcattg gacatggcgg tcagccagat cgagagacag ttcggcaaag
     1921 gcgccatcat gaagatgggt gaggcggcga accgcaaggt cgacgtcatc ccgaccggag
     1981 cactctcact cgacctcgcc ctcggtgtgg gtggtgtccc ccgcggccgc atcgtcgagc
     2041 tctatgggcc ggagagctcg gggaagacct ccctcgccct gcacatcgtg gccgaggcac
     2101 agcgcaacgg tggcatcgcc gccttcatcg acgccgagca cgccctcgac cccatctatg
     2161 ccaaggcgat cggcgtcgac gtcgacgagc tgctgatctc tcagcccgac accggtgagc
     2221 aggcgctcga gatcgccgac atgctcatcc gttccggcgc cctagacgtc ctggtcatcg
     2281 actcggtggc cgcattggtc cctcgagccg agatcgaggg cgagatgggc gacacccacg
     2341 tcggcctcca ggcccgtctc atgagccagg cccttcgcaa gctggccggc accctgaaca
     2401 aatcgaggac caccgccgtc ttcatcaatc agctccggga gaagataggc gtgatgttcg
     2461 gaagcccgga gaccaccccc ggtgggcgtg ccttgaagtt ctactcctcg gttcgtctcg
     2521 acatcaggcg catcgagtcg atcaaggacg gcaccgacag cgtgggcaac cgggtcaagg
     2581 tcaag
//
LOCUS       DASUTA010000141         2989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1080313, whole genome shotgun sequence.
ACCESSION   DASUTA010000141 DASUTA010000000
VERSION     DASUTA010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080313"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1544)
                     /locus_tag="VFZ80_02880"
     CDS             complement(<1..1544)
                     /locus_tag="VFZ80_02880"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6286403.1"
                     /translation="MNDAPPVILTVDDEPEVLRAVERDLRARYVADYRIIGAGGGQEA
                     IDTLSQLAQRGSSVALILADQRMPHVSGVDVLRASLELFPTAKKALLTAYADTDAAIS
                     AINDVSLDYYIMKPWDPPEDKLYPILDDLLEDWRAGYRPTFEGIRVVSHEWSKEAFDL
                     KAFLAMNQVPYRSHLVGTDPEAEKLMTSVGAENSDLPMVFFPDGDPLRHPSQSELAER
                     VGLRTHAESPAYDVAIIGAGPAGLAAAVYGASEGLTTLLIEASAPGGQAGQSSLIENY
                     LGFPKGISGADLARRAAAQATRFGAEILVPARVERVERKDPFFIVHLNDGATITSKAL
                     VVTSGVDYRRLKAEGVDDLAGAGVYYGTSRIEADYHRDQPMFVVGGGNSAGQAALFLT
                     RFTDDVSIVIRGDDLTASMSAYLIDNLDANPSVSVLPRTLIAEAKGKTHLESLVLEDS
                     ETGERREVPAGALFIFIGQRAQTEWLHDLVQLDERGFVKTGRDLGPLDNWNVDRDPLP
                     LEASVPG"
     gene            complement(1541..1837)
                     /locus_tag="VFZ80_02885"
     CDS             complement(1541..1837)
                     /locus_tag="VFZ80_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX6286404.1"
                     /translation="MSALTRDTSGMSTRTCSHFEVFTDAMEPSSDVCDQCVAMGSTWV
                     HLRACLECGTVGCCDNSPNRHARQHWQEHGHPLIQSIEPGESWRYCFPDHYLSR"
     gene            1946..>2989
                     /locus_tag="VFZ80_02890"
     CDS             1946..>2989
                     /locus_tag="VFZ80_02890"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX6286405.1"
                     /translation="MGVIRVAPPQLRTKEVEGHRTATWLELFYDLVFVVAVAIVAGRL
                     LEDITIAGVASYFGYFALLWWLWASHTYYADRYDTDDLVYRLLAAGQMVAVVAIAAAL
                     SPYEASSTQVFAAGYAVSRILLLIMYWRAYRHVEETRVLVKGYLIGFGLAAVVWTASA
                     FVADSVRPVLWIIALAIDLATPWVMRREQAKVPFDVSHLPERFGLFTILVLGESIAAV
                     VVGLGHSEWALVPTITAAVGVGIATSLWWIYFDNARGAVVRRDATVKRTWRPTVWIYT
                     HLPLAASLAASGVAMEIAVAESGHGPMTTSDRWLLVGSVIFVMLSLALIQTAAIPDAP
                     LQDQIVVSRLAAVP"
BASE COUNT          507 a          936 c          953 g          593 t
ORIGIN      
        1 ccggggaccg acgcctccaa cggcaacggg tcgcgatcga cgttccagtt gtccaggggc
       61 ccgagatcgc gcccggtctt cacgaaaccg cgttcgtcga gttgcaccaa gtcgtggagc
      121 cattcggtct gagctctctg cccgatgaag atgaacaacg ctccggcagg aacctcgcgg
      181 cgctcacccg tttcgctgtc ttcgaggacg agtgactcga ggtgggtctt gcccttggcc
      241 tctgcgatca gcgtcctcgg gagcactgac acggacgggt tggcatcgag attgtctatc
      301 aggtaggccg acatggaagc cgtgagatcg tccccccgta tgacgatgct gacgtcgtcg
      361 gtgaaccgag tgaggaacaa cgctgcctgg cccgccgagt tgccaccgcc caccacgaac
      421 atgggctggt ctcggtggta gtcggcttcg attcgggagg ttccgtagta gacgccggct
      481 ccggcgagat catctactcc ttcggctttg agacggcgat agtcgacgcc actcgtcacc
      541 accaaggctt tggaggtgat tgtcgcccca tcgttgagat ggacgatgaa gaacgggtct
      601 ttccgctcga ctctctccac ccgggccggg acgaggatct cggctccgaa ccgagtggct
      661 tgcgctgcgg cccgccgggc caggtcggcg ccactgatcc ccttggggaa tccaagatag
      721 ttctcgatga gagacgactg tccggcctgg ccaccgggcg ccgaggcctc gatcagcagt
      781 gtcgtcaaac cctcggaggc gccatacacg gctgcggcca acccggcagg tccggcaccg
      841 atgatggcga cgtcataggc aggagactca gcgtgagttc gcagccccac tcgctctgcg
      901 agctcgctct ggctgggatg gcgaagcggg tcgccatcgg gaaagaagac catcggcagg
      961 tcgctgttct cagctccgac gctcgtcatc agcttttcgg cctcggggtc ggttcccacc
     1021 aagtgggagc ggtagggcac ctgattcatc gccaaaaaag ccttgaggtc gaacgcctct
     1081 ttcgaccact cgtggctgac cacgcgtatc ccttcgaagg tcggccgata gcccgcccgc
     1141 caatcctcga ggagatcgtc gaggatgggg tacagcttgt cctccggcgg gtcccagggc
     1201 ttcatgatgt agtagtcgag gctgacgtcg ttgatggcgc tgatggcggc atcggtgtcg
     1261 gcataggcgg tgagaagggc cttcttcgcc gtgggaaaca gctcgagaga ggcgcgaagc
     1321 acgtcgacac ccgagacatg gggcatccgt tggtccgcca ggatcagagc gactgaactg
     1381 ccacgctgtg ccagctgact cagggtgtcg atggcttctt ggccgccgcc ggctccgatg
     1441 atgcggtagt cggcgacata gcgggctcgc aggtcgcgct cgacggcgcg gagcacctcc
     1501 ggctcgtcat cgacggtgag gattacagga ggagcgtcat tcatctcgag aggtagtgat
     1561 caggaaagca atagcgccag ctctctccgg gctcgatcga ctggatcaac gggtggccgt
     1621 gctcctgcca gtgctgacgg gcatgccggt tcggtgaatt gtcacagcac cccactgtcc
     1681 cacactcgag acaggcccgg agatgaaccc aggtgctacc catggcaacg cattgatcac
     1741 agacgtcgga ggacggctcc atcgcgtccg taaagacctc gaagtggctg caagttctag
     1801 tactcattcc gctggtgtcc ctggtcaatg cgctcatgca tcttaagttg accggcgaac
     1861 atcgcgtcgc gctgagatgc gcaggtgcgt tcggctccgc ttatcgtgtc ggctccccgc
     1921 tgatttggag gcggggagag ggtcaatggg tgtcataagg gtcgcgccgc cacaactgag
     1981 aacgaaagag gtcgagggcc atcggaccgc cacttggctc gagctcttct acgacctcgt
     2041 cttcgtcgtc gccgtagcca tcgtcgcggg gcggcttctc gaagacataa ccatcgcggg
     2101 cgtcgcctcc tatttcggct acttcgccct gctctggtgg ctgtgggcca gccacaccta
     2161 ctacgcggac aggtacgaca ccgacgacct ggtctacaga ctgctcgccg ccggccaaat
     2221 ggtggcggtc gtggcgatcg ccgccgccct gtccccgtac gaggcgagct ccacacaagt
     2281 cttcgctgcg ggctacgccg tgtcgaggat ccttctcctg atcatgtact ggcgtgcgta
     2341 ccgccacgtg gaggagacca gggtcctcgt caagggttac ctcatcggtt tcgggctcgc
     2401 cgctgtggtc tggacggcct cagccttcgt tgccgactcg gtccgaccgg tgctctggat
     2461 catcgctctc gccatcgacc tggcgacgcc gtgggtcatg cggagagagc aggcgaaggt
     2521 cccattcgac gtttcccatc tgcccgagcg cttcgggctg ttcacgatcc tggtcctggg
     2581 tgagtcgatc gctgccgttg tcgtcgggct gggtcattcc gaatgggcat tggtgccgac
     2641 tatcaccgcc gccgtcggcg tcggcatcgc cacctcgctt tggtggatct atttcgacaa
     2701 cgctcgtggg gccgtggtgc gacgcgacgc gacggtcaaa cgaacctgga gacccacggt
     2761 ttggatctac acccaccttc cgctggccgc gtcattggcc gcgtcaggag tcgccatgga
     2821 gatcgctgtc gccgaatcgg gtcatggccc gatgaccacc tcggaccgtt ggctgctcgt
     2881 gggttcagtc atcttcgtga tgctcagcct cgcgctgata cagacggcgg cgatccccga
     2941 tgctccgctg caagaccaga tcgtggtttc tcgtctcgcc gctgtgccg
//
LOCUS       DASUTA010000142         3947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_174152, whole genome shotgun sequence.
ACCESSION   DASUTA010000142 DASUTA010000000
VERSION     DASUTA010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174152"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VFZ80_02895"
     CDS             complement(<1..343)
                     /locus_tag="VFZ80_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HEX6286406.1"
                     /translation="MATALWIAAGGAVGTLARFAVTGLFDGHGHPWGTVTVNILGSLA
                     LGVLAGLWGMGHVADQQLALTVGLLGGFTTFSTFALDTIRLWENGDAVLAVVTVATSV
                     VMGLAAAIAGLA"
     gene            420..1505
                     /locus_tag="VFZ80_02900"
     CDS             420..1505
                     /locus_tag="VFZ80_02900"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX6286407.1"
                     /translation="MAVQKTQWRVLLVITLIALAGSLFPMPSGAVTEQQVDEACADSQ
                     EQLAAYREAQGAFEEAAYDYEDALNDVEFLERKQDRMQTNYDAHAEDLESVQGQLEEQ
                     AVQMYMMGGFNNPGIIFSASSVDQVMTTTEFLSSATLGGQESINDLIAAKGELSRYQE
                     DLAVVHDELKVAEANALEILDRQEEAMKAEQAAYAKLSGRCKDLTAQRAQEVAEAEAR
                     RKQAAAGSVQVGSFICPLPPGQTSFIDSWGFPRSGGRRHKGVDMFAARGTPIYAVQSG
                     TASASSSSLGGITIHLRADTGFTYYYAHLDSRAISGSQRVSQGQTIGYNGDTGNARGT
                     SPHLHFEIRPGGGGAVNPYPTVRAACY"
     gene            1516..2043
                     /locus_tag="VFZ80_02905"
     CDS             1516..2043
                     /locus_tag="VFZ80_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286408.1"
                     /translation="MSDKRRRLDQVLEPGYVEDLGDLELNEVRRRRDTAEDVEAQISY
                     YRRLLHGRMDLIDFEMRRRSGEEERSILEALPEILASGMILGSEPTLRHIETMPPLPP
                     TTGRRLIDKVMDDGVLTSLPELTEDELSEAIDRLRDVETELSGQRRRLHEVIDKLQDE
                     IVSRYRSQQDEAPVT"
     gene            2061..2993
                     /locus_tag="VFZ80_02910"
     CDS             2061..2993
                     /locus_tag="VFZ80_02910"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX6286409.1"
                     /translation="MRSGLVETIHEGAVAVVDPDGVLVARHGDIDRPFFLRSSAKPFQ
                     AHVSQQAGADLSPLQLAMACASHRGFPVHIALVQSILEGAGLDEDALGCPPHWPLGSA
                     AARIVLRGGAEEPRRIWHNCSGKHAGFLRACVAAGWPLGTYLESDHPLQQRVIDLVSE
                     VGGYPAEPVGVDGCGAPVMRTTVRAMATMFARLGTAAELADVFTSMHRYPALVAVNGQ
                     GDAEIAMATDSAAKGGAQGCVGVAVAGRYGVGVKSWDGSSRVADLAAASVLEQLGVLS
                     ATGREILEPIIHSPVLGGGRTVGRLEPSFELETV"
     gene            2990..3409
                     /locus_tag="VFZ80_02915"
     CDS             2990..3409
                     /locus_tag="VFZ80_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX6286410.1"
                     /translation="MREVLEERIRLWVDDPTSNVEYAEEVEGRWAVRMRQDVRDATTV
                     WFDVGERSLQFEAYVLPAPVTPDEVHRQALVRNIRAWRCFFAVDTEGAIVLRGRLAAD
                     RVTLDELDRVLGELFESIEVAFRPMLRAGFPSREKST"
BASE COUNT          724 a         1245 c         1283 g          695 t
ORIGIN      
        1 tggcgaggcc ggcgatcgct gcggccagcc ccatcaccac cgaggtcgca accgtcacca
       61 ccgctagcac ggcgtctccg ttctcccaca gacggatggt gtcgagcgcg aacgtcgaga
      121 acgttgtgaa accgccgagg aggccgaccg tcagagcgag ttgctgatcg gccacgtggc
      181 ccatgcccca caaaccggcg aggaccccga gggcgaggct ccccaggatg ttgacggtca
      241 ccgtccccca tgggtggccg tgcccgtcga agaggcccgt gacggcgaag cgagccaggg
      301 tccccaccgc cccacctgct gcaatccaca gtgccgtcgc cattgcgccg caggttaggt
      361 caggtttctc gagccactga ctactgcaag ggaatgagcg tggttagcat ttctcagcca
      421 tggcagtcca gaagacgcag tggcgcgtct tgctcgtcat cacactcatc gccctggccg
      481 gatcgctttt cccgatgccg tccggtgcgg tcaccgagca gcaggtggat gaggcgtgcg
      541 ccgactcaca ggagcagctc gccgcgtatc gggaagccca gggtgcattc gaggaagcgg
      601 cttatgacta cgaggacgcc ctgaacgatg tcgagttcct ggagcggaaa caggatcgca
      661 tgcagaccaa ctacgacgcc cacgccgagg acctggaatc ggtccagggt caactcgagg
      721 aacaggctgt gcagatgtac atgatgggcg ggttcaacaa cccggggatc atcttctccg
      781 cctcatcggt cgaccaggtc atgaccacca ccgagttcct cagctcggcc accctgggtg
      841 gtcaggagtc gatcaacgac ctgatcgcgg ccaaaggcga actgagccgg tatcaagagg
      901 atctcgccgt tgttcacgac gagctcaagg tggccgaagc caacgctctc gagatcctcg
      961 accgtcagga agaggccatg aaggcagagc aggcggccta tgccaagctc agcggcaggt
     1021 gcaaggacct gactgcgcag cgggcccagg aggtcgccga ggctgaggcg aggcgtaagc
     1081 aggcggccgc aggatcggtc caggtgggca gtttcatctg tccgctgcca ccgggccaga
     1141 cctcgttcat cgactcatgg ggcttccctc ggtccggggg tcgccggcac aaaggcgtcg
     1201 acatgttcgc cgcccggggc acgccgatct atgcggttca gtccgggact gcgagtgcgt
     1261 cgagcagctc actgggaggc atcacgatcc atctccgcgc cgacaccggt ttcacctact
     1321 actacgccca tctggacagt cgggccatct ccggctcaca acgggtgtcc cagggccaga
     1381 ccatcggata caacggagac actggaaacg cacgcggcac aagcccgcat ctccacttcg
     1441 agatcagacc cggtggcggg ggagcagtca acccttatcc cacggtgcga gcagcctgtt
     1501 attaacgtgg cggacgtgag tgacaagcgc cgccggctcg atcaggtcct cgaacccgga
     1561 tatgtcgagg atctggggga tctcgagctc aacgaagtcc gtcgtcggcg agacaccgcc
     1621 gaggacgtcg aggcacaaat ctcctattac cgccgcctcc tccacggtcg catggatctg
     1681 atcgatttcg agatgcggcg tcggtcgggt gaggaggagc gctccatcct cgaggcattg
     1741 cccgagatcc tcgccagcgg gatgattctc ggctccgagc ccaccctgcg ccacatcgag
     1801 acaatgccgc ctctgcctcc caccaccggg cgccggctca tcgacaaggt catggacgac
     1861 ggcgtgctca cctcgttgcc cgaattgact gaagacgagc tcagcgaggc gatcgatcgc
     1921 cttcgcgatg tcgagaccga gctctctggt cagcggcgcc ggctccatga agtgatcgac
     1981 aagcttcagg acgagatcgt gtcccgttac cggagccaac aggacgaggc gcccgtaacc
     2041 tgaacctcct cgccgcccag atccgctcgg gcctcgtcga gaccatccac gaaggagcgg
     2101 tcgcggtggt agacccagac ggagttctcg tggccaggca cggggacatc gaccggccct
     2161 tttttcttcg atcgtccgcc aagcctttcc aggctcacgt cagccagcag gcaggagccg
     2221 acctcagccc tcttcaactg gcgatggcgt gtgcctccca ccgagggttc ccggtgcaca
     2281 tcgcactggt ccaatcgatc ctcgaaggtg ccggtctcga tgaggacgcc ctggggtgcc
     2341 ctccccactg gcctctcggt tctgctgctg cccgcatcgt gttgagggga ggggctgagg
     2401 agcctcgccg gatctggcac aactgctccg gcaaacatgc cggctttctg cgggcgtgtg
     2461 tcgccgcggg ctggccactc gggacctatc tcgagtctga ccatccccta cagcagcgtg
     2521 tgatcgatct cgtgtccgag gtcggcggct atccggctga gccggtcggc gtcgatggtt
     2581 gtggcgcgcc cgtgatgagg acgacggttc gtgccatggc caccatgttc gcccggctgg
     2641 gcacggccgc agagctcgct gacgtgttca catcgatgca tcgctatccg gccctggtcg
     2701 cggtgaatgg tcagggcgac gccgagatcg ccatggcaac cgattccgcg gccaagggag
     2761 gcgcccaggg ttgtgtcggc gttgcagttg ccggacgata tggagtcgga gtcaaatcgt
     2821 gggacgggtc gagtcgggtc gccgatctgg cggctgcatc tgtgctcgag cagctcggcg
     2881 ttctatcggc caccggtcga gaaatcttgg agccgatcat ccactcaccg gtcctcggcg
     2941 gtggtcgcac cgtgggccgt ctcgagccga gcttcgagct ggagaccgtg tgagagaggt
     3001 gctcgaggag cgcatcaggc tgtgggtgga cgatcccacc tcgaatgtcg agtatgccga
     3061 ggaggtcgag ggccgatggg cggttcgaat gagacaggac gtccgagacg ccaccacggt
     3121 ctggttcgac gtcggcgagc gcagtctcca gttcgaggcc tacgtgttgc cggcgccggt
     3181 gacgccggat gaggtgcacc gccaggcact ggtgcgcaac atccgggcgt ggcggtgctt
     3241 cttcgcggtc gatacggaag gagccatagt cctccgaggc cgcctggcgg ccgatcgggt
     3301 caccctcgac gagctggatc gagtcctggg agaactgttc gagagcatcg aagtggcctt
     3361 ccgccccatg ctgcgcgcgg gcttcccgag tcgcgaaaaa tcgacttaga aacttgtgaa
     3421 cacacccttg tggacaaagc ctgtggaaaa ccccgttttc tgttggaaac cccgattctg
     3481 gcgatagtcg acactacttg tcgataatcg atgctcatac cttccaacga tggggtcatt
     3541 gatattgggg ttaccgatgc gtacattccg ccccaggcaa acgccccaga ggcaagccgg
     3601 agaagcgtcc gttcggggaa gtgcggagga ggctccggaa ggtcactggg gcgttgtcgc
     3661 gtgctcgctg cggtcggccg atccttgccg tttgtcgccg gtgggcccaa gcgaaagtcg
     3721 gccgccatct gatcgtggcc gaccggcgtc tgtttcgctg accgattgct ctgaagatcg
     3781 agtagtgagg gtgggcccag gcgacgcgat tccgtcaggc gagggagact cccagcgaaa
     3841 gtcggccacc atctaatcgt gacacatcca cccgtcacgg gtggcgaaga agcacgattc
     3901 gccgggtgtc accggcttgt ccgggcccac gatcagggtg gtggtcg
//
LOCUS       DASUTA010000143         2789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_562781, whole genome shotgun sequence.
ACCESSION   DASUTA010000143 DASUTA010000000
VERSION     DASUTA010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562781"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VFZ80_02920"
     CDS             complement(<1..570)
                     /locus_tag="VFZ80_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286411.1"
                     /translation="MGTIAFNQSSRWTPGELNVAIADQWPFGEIESVACTPIGEDYGF
                     AGHVSRLDLVTSTGPRPVVAKEETQDQIERVIGAYRQMAPALKARVPELYAWGEGFAL
                     FELVAPATQGDGLRVEPWQVEPLIRLLADLHDTTWSDEPGMWAPDHWEIGRWQARLDL
                     AEARYPDRMTKELRDRLIILHDEMPGVISS"
     gene            560..2185
                     /locus_tag="VFZ80_02925"
     CDS             560..2185
                     /locus_tag="VFZ80_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX6286412.1"
                     /translation="MVPTFAERAEVGHHTSGTVGEVTEPEQSSTEQVRAAELIASMCL
                     ATDLGMGFPFEHGLQATLVTMRLCDALGVDDETRSRTYHASLLMYVGCTVDEVERSSI
                     FPRSMTQYVTPRQFGSRAEIVAGVSRALQSDDAHLARRAWEVATGLPRAARFISGHFV
                     ALCEVAGMVAERLGVPESVHGMFDLMTERWDGTGILGRAAGDEIPLPLRIIQVGRDAA
                     FQSLQGDLEYVKKVIQSRGGKAFDPDVVSAFLADAPRLLGGQQAAGSSWDEVLASEPR
                     PWLTLQGAEIDRALSALGAFADLVSPYLTGHSSGVAELAATAAEIHGFESSDVATIRR
                     AGFVHDVGRTAVSSRVWNKEGPLTADEREKARLHPYHTERVLTHSPFLSDVASVAMAH
                     HERPDGSGYHRGLDISSLSPAARLLAAADAFQSKTERRPYREALDRRQASDLLVEKAE
                     QGAFDPAMVAAVVKAAGEEPPSLARTAGLTEREVEVIGLLARGLKTKQIARELDISPK
                     TVDRHIQNSYRKMDVSSRAAATLFAAENGLVST"
     gene            2248..>2789
                     /locus_tag="VFZ80_02930"
     CDS             2248..>2789
                     /locus_tag="VFZ80_02930"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6286413.1"
                     /translation="MSNGNRQRIDTLVIGGGQAGLAVGYHLNQARVPFQIVDANERTG
                     DTWRQRWDSLRLFTPNRFNSLPGLTIPGEDWGFPTKDEVADYLESYAKHFDFPIVHGT
                     RVDRLTRDGHRFVATAGERDLEADNVIVAMALYQEPKTPDFSDRLDSSIFQVHVDDYE
                     SPRQLNEGGVLVVGLGNSGA"
BASE COUNT          492 a          886 c          896 g          515 t
ORIGIN      
        1 cgatgagatc actccgggca tctcgtcgtg gaggatgatc agccgatctc ggagctcctt
       61 ggtcatccgg tccgggtacc gcgcctccgc caggtccaat ctcgcctgcc agcgtccgat
      121 ctcccagtga tcgggagccc acatgcccgg ctcgtccgac caggtggtgt catgaaggtc
      181 ggcaaggagt cgaatgagag gttcgacctg ccagggctcc accctgagcc cgtctccctg
      241 tgtcgcaggg gcgaccagct cgaagagcgc aaacccctct ccccaggcgt acagctccgg
      301 cacccgggcc ttcaaggccg gtgccatctg ccgatatgcg ccgataaccc tttcaatttg
      361 gtcttgggtc tcctctttgg ccaccaccgg ccgtgggccg gtggaggtga ccaagtcgag
      421 acgggagacg tggccggcga agccgtagtc ctccccgatc ggggtgcaag ccaccgattc
      481 gatctcgccg aagggccatt ggtccgcaat cgccacgttc agctcgcctg gcgtccatcg
      541 actcgattgg ttgaaagcga tggtgcccac cttcgcagaa cgcgccgagg taggtcacca
      601 cacctccggt accgttggtg aggtgacgga gccggagcag agctcgacgg aacaggtgcg
      661 tgccgccgag ttgatcgcat cgatgtgtct cgcaaccgac ctcgggatgg gctttccgtt
      721 cgagcacggc ctccaggcca ctctggtgac catgcgtctt tgtgatgcgt tgggtgtcga
      781 tgacgaaacg cggtctcgca cttaccacgc cagtcttctc atgtacgtcg gttgcacggt
      841 tgacgaggtg gagaggtcct cgatcttccc gcgcagcatg acccaatacg tgaccccacg
      901 acagttcggc tcgcgtgcgg agatcgtcgc tggggtgtcg cgggccctgc agtccgatga
      961 cgcccacctg gcccgacggg cgtgggaggt ggccaccggc ctgccccggg ccgcacgatt
     1021 catcagcggg cacttcgtcg ccctctgtga ggttgcaggc atggtggccg agcgtctcgg
     1081 cgtccccgag tcggtgcacg gcatgttcga tctcatgact gaacgctggg atgggacggg
     1141 catcctcggc cgggccgcgg gcgatgagat cccacttccc cttcggatca tccaggtcgg
     1201 gcgagacgcc gcatttcaaa gcctccaagg ggatctcgag tatgtgaaga aggtgatcca
     1261 gtctcgggga ggcaaggcct tcgaccccga tgtcgtttca gcgttcttgg ccgacgcgcc
     1321 acgactgctc ggtggccaac aggccgcggg atcgtcatgg gacgaggttc tcgcatcgga
     1381 gcccagaccg tggctcacac tccagggcgc ggagatcgac cgtgcgttga gtgcgctcgg
     1441 ggcgtttgcc gatttggtct cgccttatct cacagggcac tcctcgggcg tggccgagtt
     1501 ggccgccacg gcggcagaga tccacggttt cgagtcctcg gacgtggcga cgatccgccg
     1561 cgccgggttc gttcatgacg tcgggcgaac ggcggtctcc tcacgcgtct ggaacaagga
     1621 agggcccctc accgcagacg agagggagaa ggcgaggctg cacccgtatc acaccgaacg
     1681 cgttctgacc cactcaccct ttctctccga cgtggcatcg gtggcgatgg cacaccacga
     1741 gcggcccgat ggctcgggtt atcaccgtgg cctcgacatc tcttcgctct cgcctgccgc
     1801 caggctgctt gcggcagccg atgcctttca atcgaagacc gagcgacgtc cctatcgaga
     1861 ggcgctcgac aggcgacagg cttcggacct actcgtggag aaggcggagc aaggcgcctt
     1921 cgacccggcg atggtcgcag ctgtggtgaa agcagccggt gaggaaccgc cctccttggc
     1981 ccggacggca gggttgaccg agcgcgaggt ggaggtgatc ggtcttctcg ctcgtgggtt
     2041 gaagaccaag cagatcgctc gcgagctcga catctcaccc aagaccgtcg accggcacat
     2101 ccaaaacagc taccgcaaga tggatgtctc ctcgcgcgca gcagcgaccc tcttcgccgc
     2161 tgagaacggc ctggtatcga cctgatctgc cccgtatggg gagttttccg tattcacggg
     2221 cgtgcgctcg tgccttacgg tcgaagcatg agcaacggaa accgacaacg tatcgacacc
     2281 ctcgtcatcg gaggtgggca ggccggtctg gctgtcggct atcacctgaa tcaggctcgg
     2341 gtgccgtttc aaatcgtcga tgccaacgag agaacaggtg acacctggcg acagcggtgg
     2401 gactcattgc ggctcttcac gcccaaccga ttcaacagcc tgcccgggct caccatcccc
     2461 ggtgaggact ggggcttccc cacgaaggac gaggttgccg actacctgga gtcgtacgcc
     2521 aaacatttcg attttccgat cgtgcacggt acgcgtgtgg atcgcctgac cagggacggc
     2581 cacaggtttg tggcgacggc aggcgagaga gatctcgagg ccgacaatgt gatcgtggcg
     2641 atggccctct accaggagcc gaagacgcct gacttcagcg accgcctcga ctcgagcatt
     2701 ttccaggtgc acgtcgacga ctacgagagc cctcgacagc tgaacgaagg gggcgtgctg
     2761 gtggttggcc tcggaaactc aggtgctga
//
LOCUS       DASUTA010000144         2054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_174767, whole genome shotgun sequence.
ACCESSION   DASUTA010000144 DASUTA010000000
VERSION     DASUTA010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174767"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="VFZ80_02935"
     CDS             <1..1235
                     /locus_tag="VFZ80_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX6286414.1"
                     /translation="LFAALYVLLELLTVEVNDRMFQSSSIMVLMTAGVIFATQPSSDA
                     VFALALMAGLGATVPDDFKERRWFQPMANFGQQAVSGALAGFILDVTLGGIGTPTSSD
                     LLIIALASSAGALVFALVNNFLVRRAVQVVYPYDRLQPWSHLGTLFAGQFVMGLLGGL
                     IGGAYVIADREALLVLIIGVYAIGHLSLYAFSQLRVSHISAIKGFVKTLEAKDMYTRG
                     HTERVAYFAEMICEEYGFNGTQIEEVKWAALIHDLGKLAVPTELIRKRGRLDDEEYSQ
                     MKEHAHLVEEILAEVDFLQPMVEIASGHHSHYDGNGYGGRGHIEGETPNLKTCIVAVA
                     DAFDAMTSARSYRMALSQRYALSELRKHSGAQFHPEVVDMFERALQKTGRRWGAPHVN
                     DEEMARRLAEEPELTLRG"
     gene            1270..>2054
                     /locus_tag="VFZ80_02940"
     CDS             1270..>2054
                     /locus_tag="VFZ80_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286415.1"
                     /translation="MFPLASLALGVYCAFLAEANPLYIVVAAVALVIGSLVGVATSNS
                     GNHFTMGLAVAAAIPFVFSSLDVVEPFASVASFGFGLAGVWAVRHIRGDGHRELLPVM
                     VRRLCGYVVYGVVYSAMRVGLFADLDGGWEDLFPFAVATIAWLIVEVSLRALLVIGPR
                     ELSRSYLARALLHDLNVFMGLTLTGAVFGELFAPIGWWALPIALLPYSFAHIAFRRFQ
                     ETKTTYRQTIRALARIPEVSGLGIDGHADRTTEMATSIAKEMGL"
BASE COUNT          334 a          647 c          611 g          462 t
ORIGIN      
        1 tcctattcgc ggctctctac gtcttattgg aattgttgac cgtcgaggtc aacgatcgga
       61 tgttccagtc ttcttcgatc atggtgctga tgacagccgg ggtcatcttc gcgactcagc
      121 ccagctccga cgcagttttt gcactagccc tcatggctgg tctcggagcg actgtcccgg
      181 atgacttcaa ggaacgacgt tggttccaac ccatggcgaa cttcggccag caagctgtat
      241 cgggggccct ggcaggcttc attctcgacg tgaccctcgg ggggatcggc acaccgacgt
      301 cgtcggatct gttgatcatc gctctcgcgt catcagcggg ggcactcgtg tttgctttgg
      361 tgaacaactt cttggtccga cgtgcggttc aggtcgtcta cccgtatgac cgtctccagc
      421 cctggtccca tctgggaacc ctcttcgccg gccagttcgt catgggcctc ctcggcgggc
      481 tgatcggcgg cgcctacgtc atcgccgacc gtgaggctct gctcgtcctc atcatcgggg
      541 tctatgccat cggtcacctg tctctgtacg ccttctccca acttagggtg tctcacatct
      601 ctgcgatcaa gggcttcgtc aagaccctcg aggcaaagga catgtacaca cgaggtcaca
      661 ccgaacgggt cgcctacttc gccgagatga tctgtgagga atacggcttc aacggcaccc
      721 agatcgaaga ggtcaaatgg gctgccctca tccacgactt gggaaaactg gctgtgccca
      781 ccgagttgat tcggaagagg ggtcgtctcg acgacgagga atacagccag atgaaggagc
      841 acgcccatct ggtcgaggag atcctcgctg aggtcgattt cctccaaccc atggtcgaga
      901 tcgcctccgg ccatcacagt cattacgacg ggaacggata cgggggccgg ggtcacatcg
      961 agggtgagac cccgaacctc aagacttgca tcgttgcagt ggccgacgcc ttcgatgcga
     1021 tgacgtcggc tcgctcttac cgcatggccc tgtcacaacg ctatgccctg tctgagctgc
     1081 gcaagcactc cggagcccag ttccatcccg aggtcgtcga catgttcgaa cgggccttgc
     1141 agaagacggg tcggcgctgg ggtgcaccac atgtcaatga cgaggagatg gcccgccgtc
     1201 tcgccgagga gccggagttg acgcttcgtg gctaggtggc atccccgcgt ccttgcgatc
     1261 gtctcctgga tgttccctct tgcctcgttg gcgctcggcg tctactgcgc gttcctggca
     1321 gaggccaacc ctctctacat cgtggttgct gctgttgccc tggtgatcgg atctctggtg
     1381 ggtgtcgcca cctccaactc ggggaaccac ttcaccatgg gtctggcggt ggctgccgcg
     1441 atcccattcg tcttctccag tctcgacgtt gtcgagccat tcgcctctgt agcgtcattt
     1501 ggcttcgggc tggccggggt atgggcagtc cgacacattc gtggcgacgg tcaccgggaa
     1561 ctgctccccg tgatggtgcg ccgtctttgc gggtacgtcg tctacggcgt ggtctattcc
     1621 gcgatgcgtg tcggcctctt tgccgacctg gacggaggtt gggaggacct ctttccgttc
     1681 gccgtcgcca cgatcgcatg gctcatcgtc gaggtctctc tgagggccct gctggtcata
     1741 gggccccgtg agctttcccg cagctacctg gcccgtgcgt tgctgcacga tctcaacgtg
     1801 ttcatggggc tgactctcac cggcgccgtg ttcggagagc tgttcgcccc gatcggctgg
     1861 tgggcgctgc cgatagccct gcttccgtac tccttcgcac acatcgcctt cagacggttc
     1921 caggagacca agaccaccta tcggcagacc atcagggctc tggcccggat ccccgaggtc
     1981 tccggcttgg gcatcgacgg tcatgcagac cgaacgaccg agatggccac ctcgatcgcc
     2041 aaggagatgg gact
//
LOCUS       DASUTA010000145         2022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_304507, whole genome shotgun sequence.
ACCESSION   DASUTA010000145 DASUTA010000000
VERSION     DASUTA010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304507"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..783
                     /gene="clpP"
                     /locus_tag="VFZ80_02945"
     CDS             154..783
                     /gene="clpP"
                     /locus_tag="VFZ80_02945"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX6286416.1"
                     /translation="MTTENQFQNYVIPTVFEQTSRGERYFDIYSRLLKDRIIFLGTPI
                     DDTIANLIMAQLLHLESEDPDKDIFLYINSPGGSITSLFAIYDTMQYIKPDVNTVCMG
                     MAASAAAVLLAGGAPGKRFALPHARVMLHQPHGGAQGQASDIEIQARLIVQMREQLNQ
                     ILAEHTGQDYDKIATDTERDYWMLAEEAKDYGIVDAILTRQELSAVASE"
     gene            795..>2022
                     /gene="clpX"
                     /locus_tag="VFZ80_02950"
     CDS             795..>2022
                     /gene="clpX"
                     /locus_tag="VFZ80_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX6286417.1"
                     /translation="MARLGEGGELLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEEFSTQEEVSFTELPKPAEIDSYLDDYVVGQEVAKKKLAVAVYNHYKRIRSGELR
                     RDDIELQKSNILLIGPTGCGKTLLAQTLARQLNVPFAIADATTLTEAGYVGEDVENIL
                     LKLLQAADYDVKRAEQGIIYIDEIDKISRKAENPSITRDVSGEGVQQALLKILEGTVA
                     SVPPQGGRKHPHQEFIQLDTSNVLFIVAGAFAGLEDVIANRVGKRGVGFGAELRSRAE
                     QRPIELLSQVLPEDLIKFGLIPEFVGRLPVVATVEDLDKESLVRILTEPKNALTRQYA
                     RMFDMDGVELVFTPDALEAVSEQAIKRGTGARGLRAILEEVLLSTMYDLPSRDDIGRV
                     IIDRQVVEERVNPTLVP"
BASE COUNT          423 a          654 c          607 g          338 t
ORIGIN      
        1 catcctctcg gcagcaaacc cggtcgatga agacggcaac ccggtcgatt tgaaactgaa
       61 cgtgacagag gtcgaagccg agatcgtcga ggccgagccg gtcggggaga tcccgacggg
      121 cgaggttctc cacgcagtgg cggaggaaga agaatgacaa ccgagaatca attccagaac
      181 tatgtgatcc ccaccgtctt cgagcagacc agtcggggtg agcgctactt cgacatctac
      241 agccgtctgc tcaaggatcg catcatcttc ctcggcaccc cgatcgacga caccatcgcc
      301 aacctgatca tggcccagct gctccatctc gagtcggagg acccggacaa ggacatcttt
      361 ctctacatca actcgcctgg tggctccatc acctcgttgt tcgccatcta cgacaccatg
      421 cagtacatca agcccgacgt gaacaccgtg tgcatgggca tggccgcctc ggcggcggca
      481 gtcctgctgg cgggaggagc gcccggcaag cgcttcgcgc tgccccatgc ccgggtgatg
      541 ctccaccagc cccacggcgg ggcccagggc caggcttcgg acatcgagat ccaggccagg
      601 ctgatcgtgc agatgcgtga gcagctaaac cagatcctcg ccgagcacac cggtcaggac
      661 tacgacaaga tcgccaccga caccgaacgg gactactgga tgctcgccga ggaggcgaag
      721 gattacggga tagtcgacgc aattctcacg cgtcaggaac tgtctgcggt agcctcagag
      781 taggaggatc caatatggcc cgactcggag aaggcggaga gctgctgaaa tgcagcttct
      841 gcggtaagtc gcaaaagcag gtcaagaagc tcatcgccgg tcccggtgtc tacatctgcg
      901 atgagtgcat cgacctgtgc aacgagatca tcgaggagga gttctccacc caggaggagg
      961 tctcgttcac cgagctcccc aagccggcgg agatcgactc ctacctcgac gactacgtgg
     1021 tcggccagga ggtcgccaag aagaagctgg cggtggcggt ctacaaccac tacaagcgga
     1081 tccgcagcgg cgagctccgt cgtgacgaca tcgagcttca gaagtccaac atcctcctga
     1141 tcgggcccac cgggtgcggg aagactttgc tggcgcagac gctggcccgc cagctcaacg
     1201 ttcccttcgc catcgccgat gccaccaccc tcaccgaagc cgggtatgtg ggtgaggacg
     1261 tcgagaacat cctcctcaag cttctccagg ccgccgacta cgacgtcaag cgggccgagc
     1321 aggggatcat ctacatcgac gagatcgaca agatcagtcg aaaggccgag aacccctcga
     1381 tcacccggga cgtgtccggc gagggtgtcc agcaggcgct cttgaagatc cttgagggga
     1441 cagtcgcttc ggtgccgccc cagggcggac gtaaacatcc ccaccaggag ttcatccagt
     1501 tggatacctc caatgtcctc ttcatcgtgg ccggcgcctt cgccggcctc gaggacgtca
     1561 tcgccaaccg ggttggaaag cgcggcgtcg ggttcggggc cgagctccgt tcgcgtgccg
     1621 agcagaggcc catcgagctc ctctcccagg tccttcccga agacctgatc aaattcgggc
     1681 tcattcccga gttcgtcggc cgtctgccgg tggtggccac cgtcgaggac ctcgacaaag
     1741 aatcgctcgt acgcatcctc accgagccga agaacgctct cacccgccag tacgcccgga
     1801 tgttcgacat ggatggcgtc gagttggtct tcacccccga cgccctcgag gcggtctccg
     1861 agcaggccat caagcggggc acgggagcca gaggcctgcg ggccatcctc gaagaggtgc
     1921 tgctgtccac catgtatgac ctcccgtcgc gtgacgacat cggcagggtc atcatcgacc
     1981 gacaggtagt ggaggagcgt gtcaacccca cgctggtgcc gc
//
LOCUS       DASUTA010000146         3314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_563688, whole genome shotgun sequence.
ACCESSION   DASUTA010000146 DASUTA010000000
VERSION     DASUTA010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563688"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /gene="dinB"
                     /locus_tag="VFZ80_02955"
     CDS             complement(<1..939)
                     /gene="dinB"
                     /locus_tag="VFZ80_02955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF003015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX6286418.1"
                     /translation="MEPNILHADLDAFYASVEQLLNPKLRGRPVLVGGGIVVAASYEA
                     RAFGVRAPMATRDALAMCPGAIVVDGHFDRYLELSEQVMSILDDATPIVEQISVDEAF
                     LDVSGTTHLLGSSADIARTIRCRVREEVGLPISIGVSSTKFLSKIASARAKPDGLVVV
                     ECGTELDFLHPLPVEALWGVGRVTAARLHRLGVTTIGELASVRSTALTPVLGPGASGA
                     LHSLSWNRDPRHVQRGRRAGSVGSQQALGHGLTDRSEMAVVVLGLADRVGRRLRAKDR
                     MGSTLTARVRFPGPRAVTRSHTLGAPTGSTAALSNLG"
     gene            complement(1131..2624)
                     /locus_tag="VFZ80_02960"
     CDS             complement(1131..2624)
                     /locus_tag="VFZ80_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEX6286419.1"
                     /translation="MTEWPPVLNAYEFFLAERIREGRGSRPALRLDDRIVTYLEVDEL
                     TAGFTNALHDGGVTRGARVLLVMPDGPEFAAGLLGIMRLGAVAVMLNPGLRSDALSAI
                     VDLSEASAALVDASYIDQFEAAGSSWRPYVIDPGSVAPSAVGHPTVDTVPDDPAFWLF
                     SGGTTGVPKAVVQTHKSLVNSSRLYGQGSLRMTESDVTMAVPKLYFGYATGSNLLFPF
                     SVGASSVLFEEHPTPEALFRRIEQHRPTVLVNVPSAINQMISAPGAEQRDLSSLRLAT
                     SAGEALPVSLYHRWKEIFDVELLDGLGTAEMWHIFITNTVEDVNPGTLGKVVPGYEVK
                     ACDEAGNEVAPGEVGRLWVRGDSLALGYYGNPEQTAEAFRGQWAVAGDLVSIDSEGYV
                     THRGRADDAIKVKGKWFRPQEVESVLLEHEAVKECAVIAVDDEDGLARPVAFVVTTSD
                     VSEQDLIEWTLERLEAYKHPRSVYFVDELPQTHLGKIDRGALKRAAR"
     gene            2649..>3314
                     /locus_tag="VFZ80_02965"
     CDS             2649..>3314
                     /locus_tag="VFZ80_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6286420.1"
                     /translation="MHIAMVTGPSSGIGKATALALARLGYHVVAAGRSEERTVPVVEQ
                     IVSEGGSAEFLNMDLASLESAREAASRFARSGRTLDVLVNNAGVGGTRGTTNDGFEIH
                     FGVNHLGHFMLTRHLEPSFRQGSRIVVVSSEVHRRADGIDWERVQKKSRSIAGFTEYG
                     ISKLANILFARRLAQLHPEWNSYSLHPGTVNTDIFPALVRPFFRNRLTPEQGAETSVW
                     CATA"
BASE COUNT          639 a         1101 c         1031 g          543 t
ORIGIN      
        1 gccgaggttg gacagagccg ccgtcgaccc ggtgggagcc cccaacgtgt ggcttcgagt
       61 caccgcccgt ggccccggga acctgacccg cgcagtcagc gtcgatccca ttctgtcctt
      121 ggcgcggagg cggcggccaa ctcgatcggc gagaccgagc acaaccacgg ccatctcgct
      181 gcgatcggtg agaccgtgcc ccaacgcttg ttggctgccg accgaccctg ctcgccgtcc
      241 tctttggacg tgcctggggt cccgattcca cgacaaggag tggagggcgc cagaggcacc
      301 gggtccgagg acgggggtca atgcagtgga tcgaaccgag gcgagctccc cgatggtggt
      361 cacaccgagc cgatgcaacc tggccgcggt gacccgaccc accccccaga gcgcctcgac
      421 cggcagcgga tggaggaagt cgagctctgt tccgcattcg accaccacca ggccgtccgg
      481 tttggctcgg gcggaggcaa tcttggacag gaacttcgtc gaggacaccc cgatcgagat
      541 gggaagcccg acttcctctc tcacccggca tctgatggtg cgggcgatgt cagccgagga
      601 cccgagcagg tgagtggtcc ccgagacgtc gagaaaggcc tcgtcgaccg agatctgctc
      661 cacgatgggg gtggcatcgt cgaggatcga catcacctgc tcggagagct cgagatatcg
      721 atcgaagtgg ccgtcgacca cgatggcccc agggcacatg gcgagagcat cgcgggtcgc
      781 cataggggcc ctcaccccga aagcacgtgc ctcgtaggag gcggcgacaa cgatgccgcc
      841 gcccacgaga accggccgcc cccgcaactt ggggttcaac aactgctcca ccgacgcgta
      901 gaaagcgtcg agatcggcat ggaggatgtt gggctccacc taccggatca tgccaatcgt
      961 gtgtgacaag aacctcgcgc aactgcgaac ccggggttcc tcaggccata tacggcgcaa
     1021 atcagctcat ctacaacgat gcgccaacat gagcgaatct gggtcatgcc aacttgagcg
     1081 gatctgggtc atatgtggcc cggggaaccc cgggttcgag gaatgggagg tcaacgggca
     1141 gctcgtttca gggcgccgcg gtcgatcttg ccgagatggg tctgtggaag ctcatcgacg
     1201 aagtagaccg agcgagggtg cttgtaggcc tcgagacgct ccagggtcca ctcgatcagg
     1261 tcctgttcac tgacgtcgga ggtcgtcacc acgaatgcca cgggtcgcgc cagcccatcc
     1321 tcgtcgtcga cggcgatcac cgcgcactcc ttgacagcct cgtgctcgag gagcacagac
     1381 tccacttcct ggggacggaa ccacttcccc ttcaccttga tcgcatcgtc ggcacggcca
     1441 cgatgggtta cgtagccctc gctgtcgatg gagaccagat cgccggcgac tgcccactgc
     1501 ccccgaaacg cttccgccgt ctgttcgggg ttgccgtaat agcccaaggc gagcgagtcg
     1561 ccgcgcaccc acagccggcc cacctctccg ggagcaacct cgttcccagc ctcgtcgcat
     1621 gccttgacct catatccggg cacgaccttt cccagcgttc ccgggttcac gtcttcgact
     1681 gtgtttgtga tgaagatgtg ccacatctca gcagtgccga gcccatcgag cagctcgaca
     1741 tcgaagatct ccttccaccg gtgatagagg ctcacaggga gagcctcccc ggccgaggtg
     1801 gccagccgaa gactcgacag atctctctgc tccgcacccg gagccgagat catctgattg
     1861 atcgccgaag ggacattgac gagcaccgtc ggtcggtgtt gctcgatccg cctgaacagg
     1921 gcctcgggcg tcggatgctc ctcgaacaag accgaagacg ctcccaccga gaagggaaac
     1981 aagaggttcg atccggtggc ataaccgaaa tagagcttcg gcacggccat cgtcacgtcc
     2041 gactcggtca tgcgaagcga cccctgcccg tagagacggc tgctgttgac caagctcttg
     2101 tgtgtctgaa ccaccgcctt gggcacgccc gtggttcctc cggagaagag ccagaacgcc
     2161 gggtcatcgg ggaccgtatc aacggtcgga tgacccacgg ccgatggggc gaccgagccc
     2221 gggtcgatca cgtaggggcg ccaactcgac cctgccgcct cgaactggtc gatgtaggag
     2281 gcatcgacca gcgcagccga cgcctccgag agatccacga tcgcagacag agcatccgat
     2341 ctcagccccg gattgagcat cacggcgacc gcccccagtc gcatgatgcc gaggagcccg
     2401 gccgcgaact cgggcccgtc gggcatcacc agcaagaccc gggcgccacg ggtcaccccg
     2461 ccatcgtgga gagcgttggt gaatccggcg gtgagttcgt cgacctcgag gtaggtgaca
     2521 atccggtcgt ctagccgcag cgcaggccgg ctgcccctgc cctcccggat ccgctcggcc
     2581 aggaagaatt cgtacgcgtt cagaaccggg ggccactcag tcacggcgcc aaatgtacgg
     2641 tgacccacgt gcacatcgca atggtcaccg gcccctcatc cggtatcgga aaggcgacag
     2701 cactggccct cgcccgtctc ggatatcacg tcgtggccgc aggtcgttcc gaggagcgca
     2761 cggtgcccgt ggtcgagcag atcgtgagcg aagggggatc ggccgagttt ctcaacatgg
     2821 acctggcatc gctcgagtcc gcccgggagg cggccagtcg attcgccagg agcggcagga
     2881 ccctcgacgt cctggtgaac aacgccgggg tcggaggcac ccgcgggacc acgaatgacg
     2941 ggttcgagat ccacttcggt gtcaaccatc tgggccattt catgttgacc cggcacctgg
     3001 agccctcctt ccggcagggc tcacggatcg tggtggtcag cagtgaggta caccggcgcg
     3061 ccgacggcat cgattgggag cgggttcaga aaaagagccg atcgatcgcc gggttcaccg
     3121 agtacggcat ctcgaaactc gccaacatcc tgttcgcacg gcgtcttgcc cagctgcatc
     3181 cggaatggaa cagctattcg ctgcaccccg gcactgtgaa caccgacatc ttccccgctc
     3241 tcgtacgccc attcttccga aatcgcctca ccccggaaca aggagccgag accagcgtct
     3301 ggtgtgcaac tgca
//
LOCUS       DASUTA010000147         3949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1341164, whole genome shotgun sequence.
ACCESSION   DASUTA010000147 DASUTA010000000
VERSION     DASUTA010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341164"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..174
                     /locus_tag="VFZ80_02970"
     CDS             13..174
                     /locus_tag="VFZ80_02970"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX6286421.1"
                     /translation="MIVEGRTDDGLIEAARFNGDWWALGVQWHPERMDGAHQEIFAAF
                     RRAISGEGS"
     gene            complement(293..631)
                     /locus_tag="VFZ80_02975"
     CDS             complement(293..631)
                     /locus_tag="VFZ80_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6286422.1"
                     /translation="MKLLELEVTDLCCTPLHDGAIDEERAQAMAPVLAALADPVRLRI
                     VSMLLAAPEGTSCGCDLEEPLGLAQPTVSHHLKILREAGLVEGEKRGRWVHYRVLPER
                     LAEISTALVS"
     gene            complement(816..1592)
                     /locus_tag="VFZ80_02980"
     CDS             complement(816..1592)
                     /locus_tag="VFZ80_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX6286423.1"
                     /translation="MVFALPHVVPGDDIRVSASTYVAWKKCPESANVRLQGMYGPDTR
                     PAFLGSLAHRIFSRHLTSGPIEDDEFARACREEIGGSNLNHKLGPLEMKPSQLAGVIE
                     EVRGLYARFVNFPGEGFDSSEVSLDHEADGGVNLVGTIDAVFREDLGGHRLVDWKTGE
                     IGEAEDQLLFYALLWAIDHEELPAYVEAVSVKTGERYRTVPSSADVRRVAAEIGLMVS
                     EIRKSWAEGLALERHGGPWCRYCPILDDCAEGRATAALLG"
     gene            1700..3637
                     /locus_tag="VFZ80_02985"
     CDS             1700..3637
                     /locus_tag="VFZ80_02985"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX6286424.1"
                     /translation="MKNQLSFRIHPLLAAVIVVGGVFVVLFGAYGLTRWASAGEVMGR
                     VYIAEADVGGRSEEDAQSVADSLEIGRLARQATFEVDDNAVSLQPWETGLSIATEKAV
                     DEAMAVGRTGNFANQFLFWLGNIFSTTHIELEGSLDPDAIDDVFDIWDTEVIDKPINM
                     GGVELVDGELRAVYPAEGVGVDRSPAEDIVLETLIAEESTAATIPTTTVVPELTEVEV
                     DSALAEAQQMLAGSVQMTYDEQTAVFSQDQLKEAFKSETVTNSPAQIVNSFDPAVVDQ
                     LLEPLRSEFEAEPKNAEYVINDNLTVSIQPGSNGTRIDEVETAERLYEASLSPSRTGE
                     IPIVEGAEPDVTTEELEELNVQHLVAQFTTYHSCCEPRVTNIHKIADAVDGVLLKPGE
                     TFSLNGHVGERTLEKGYVDAGSIVAGEIVDTIGGGTSQFTTTMYNAVFWGGYQDVEHK
                     PHSYYFSRYPEGIEATLFWQSIDLKFRNNREHGILIDTLYTDTSITVRFFGFNHGRTL
                     IGEQSGGETHIRVASEGGPEALHVKGETSERFDITEPGAPEFRGNPELGVNQQQTVQE
                     PAEGWTVRVTRTILRGGTEQIEEQQWVWRYRPQLEIIEVHPCKVPGSSTSCPTTTTTT
                     TAPPPPTTSTTVGEGGSTTTP"
     gene            complement(3641..>3949)
                     /locus_tag="VFZ80_02990"
     CDS             complement(3641..>3949)
                     /locus_tag="VFZ80_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286425.1"
                     /translation="EIKDSVTLGEVLVIDTDRSFTGQDGHTITPGVNGHGVPGRLAER
                     LFGLELGIDHVYVLQNTVTVRRGSPWDEESAAAVADVTASFLRYYPDDEDTAGSEEEE
                     "
BASE COUNT          769 a         1302 c         1216 g          662 t
ORIGIN      
        1 ctgtccgacg atctgatagt cgagggccgc accgacgacg gcctgatcga agcagcccgg
       61 ttcaatggcg actggtgggc cttgggtgtg cagtggcacc cggagcgaat ggatggagcc
      121 caccaagaga tcttcgccgc cttccgtcgg gcgatcagtg gggaggggtc gtagcgggtt
      181 gtagctgcgt gttgcctcgc gcgagccgcc tcgtcgccgc tgcccctcaa cgctgcggcc
      241 tggagcacga gagggcggcg ccagatcacg caccgcgact agaccgctcg actcaggaca
      301 ccagagccgt gctgatctcc gcgagacgct ccggcagtac ccgatagtgg acccagcggc
      361 cacgcttctc tccctcgacc agcccggcct cccgcaggat cttgagatga tgtgagaccg
      421 tcggctgtgc cagccccaat ggctcctcca gatcacaacc acacgaggtc ccctcggggg
      481 cggcaagcag catcgacacg atgcggagac ggaccgggtc ggccagggcg gccaggaccg
      541 gagccattgc ctgcgcccgc tcctcgtcga tggcaccgtc gtggagtggt gtgcagcaga
      601 gatcggtgac ttcgagttcc agcagcttca ccggaccgag tatacattga caagcgtcga
      661 tgtatcgaca atgatcgata aatcgaagtt cgtcgatatg tcaaaaggag gcacgatgac
      721 acttcgcaac gagaccggcg gctgttgtgg cggcggaggc tgcccctgct gctagccggt
      781 taggagacgg cggcgttgtt gctcacccgg atgggtcatc ccaacaacgc tgccgtcgcc
      841 cttccttcgg cgcagtcgtc gaggatcggg cagtatcggc accacggccc gccatgtcgc
      901 tccaaggcaa gcccctcggc ccatgacttg cgtatctcgc tgaccatcag accgatctcg
      961 gcagcaaccc gtcgcacatc cgcgctgctc gggaccgttc ggtagcgctc ccccgtcttc
     1021 accgacaccg cttccacata ggcgggcaac tcttcatgat cgatggccca gagcagggca
     1081 tagaagagga gctgatcctc ggcttcgccg atctctccgg tcttccagtc gacgagcctg
     1141 tgcccgccca gatcctcgcg gaagacggcg tcgatggtgc cgaccagatt cactccaccg
     1201 tcggcctcgt ggtccaggga cacctcggag ctgtcgaacc cctctccggg gaagttgacg
     1261 aaacgcgcgt agaggccgcg gacctcctcg atgaccccgg cgagctggga gggcttcatc
     1321 tcgagtgggc ccagcttgtg gttcaggttc gacccaccta tctcctcgcg gcatgcccgt
     1381 gcgaactcgt cgtcttcaat cggtcccgac gtcaagtgtc gggagaagat tcgatgggcc
     1441 aggctgccca gaaacgccgg ccgggtgtcg ggcccgtaca tgccctgcag tcggacattt
     1501 gcgctctccg ggcacttctt ccaggcgaca tacgttgatg cactgacacg tatgtcgtct
     1561 ccagggacca catgaggcaa ggcaaagacc atcccgaccg agcgtatacc ccgccaccga
     1621 cggattggat tgagcaaacc tcctgcacac cggtactatc gaaagctcac ggttcgggcc
     1681 tgttggggcg gccccccaca tgaagaatca gctctcgttt cggatccacc cgcttcttgc
     1741 cgccgtaatc gtcgtaggcg gtgtgtttgt ggtgctcttt ggcgcttacg gcctgacgcg
     1801 ttgggcttcc gcaggcgagg tgatggggag ggtctacatc gccgaggccg acgttggcgg
     1861 gcgcagcgag gaggatgctc aaagcgtcgc agattcgctg gagatcgggc gtttggcccg
     1921 acaggcgacc ttcgaggtcg acgacaacgc ggtgagcctc caaccgtggg aaacagggct
     1981 ctccatagcc acggagaagg ccgtcgacga ggcaatggcc gtgggccgca ccggaaactt
     2041 cgccaaccaa ttcctcttct ggctcgggaa catcttcagc acgacccaca tcgagctgga
     2101 gggcagcctc gaccccgatg ccatcgacga cgtgttcgac atctgggaca ccgaggtgat
     2161 cgacaagccg atcaacatgg gcggtgtcga gctcgtcgac ggagagctca gggccgtcta
     2221 cccagcggag ggcgtgggcg tcgatcggtc accggccgag gacatcgtcc tcgagaccct
     2281 catcgctgag gaatcgacgg cggcgaccat ccccaccacg accgtcgtcc cagagctgac
     2341 cgaggtcgag gtcgattctg ctctagccga ggcccagcag atgctggctg gatcggttca
     2401 gatgacgtac gacgaacaga ccgccgtctt ctcccaggac cagctcaaag aggccttcaa
     2461 gtcggagacc gtcaccaaca gcccggcgca gatcgtcaac tcgttcgacc ctgctgtcgt
     2521 cgaccagctt ctggagccgt tgcgctccga gttcgaggcg gagcccaaga acgccgaata
     2581 cgtcatcaac gacaacctca cagtctcgat ccagcctggc agcaacggca ccaggatcga
     2641 cgaggtggaa acggccgagc gcctatacga ggccagtctg agcccctcac gaaccggcga
     2701 gatcccgatt gtggagggcg ccgagcccga cgtgaccacc gaggagctcg aagagctcaa
     2761 tgtccagcac ctggtggccc agttcaccac ctatcacagc tgctgtgagc cccgtgtcac
     2821 caacatccac aagatcgccg acgctgtcga cggtgtcctg ctcaaaccgg gcgagacatt
     2881 cagcctgaac ggccatgtcg gtgagcggac cctggagaag ggatatgtcg acgccggttc
     2941 gatcgtggcc ggggagatcg tcgacaccat cggaggtgga accagccagt tcaccaccac
     3001 catgtacaac gcggttttct ggggcggcta ccaggacgtc gagcacaagc cccactccta
     3061 ctacttctcc agataccccg agggcatcga ggccacgctg ttctggcagt cgatagacct
     3121 caagttccgc aacaaccgtg agcacggcat cctcatcgac accctctaca cagacacatc
     3181 gatcacggta cgtttctttg ggttcaacca cgggcggacc ctgatcggtg agcaaagcgg
     3241 tggggagacc cacatccgag tcgccagcga aggcggtcca gaggcactgc acgtcaaagg
     3301 cgagacctcg gagcgcttcg acatcaccga accgggtgct cccgagttca ggggaaaccc
     3361 cgagctgggc gtcaaccagc agcagacggt gcaggaaccg gcggaggggt ggaccgtgcg
     3421 ggtcacccgg accatcttgc ggggagggac cgaacagatc gaggaacagc agtgggtgtg
     3481 gcgttatcgg ccccagctcg agatcatcga ggtccacccg tgcaaggtcc cgggttcgag
     3541 cacatcgtgc ccgacgacaa cgaccaccac gaccgctccg ccaccgccca caacctccac
     3601 caccgtgggc gaaggcggct ccacgaccac cccctgagcc ctactcctct tcttcggagc
     3661 cggcggtgtc ctcgtcgtcg gggtagtacc tgaggaaaga ggcggtcaca tcggccacgg
     3721 ctgccgccga ctcctcgtcc cacggggagc cccgtctcac cgtcaccgtg ttctgcagca
     3781 cgtagacgtg gtcgatgccc agctcgaggc cgaagagacg ctcggccaac cgacccggca
     3841 caccatggcc gttgacgccg ggggtgatgg tgtgcccgtc ctgaccggtg aagctgcggt
     3901 cggtgtcgat gaccagcacc tcaccgagcg tcacactgtc cttgatctc
//
LOCUS       DASUTA010000148         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1083171, whole genome shotgun sequence.
ACCESSION   DASUTA010000148 DASUTA010000000
VERSION     DASUTA010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083171"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..667)
                     /locus_tag="VFZ80_02995"
     CDS             complement(14..667)
                     /locus_tag="VFZ80_02995"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6286426.1"
                     /translation="MSYPHEGARRRGEMILDLIAELGLSRPRILDLGCGTGWLTPRLA
                     ELGEAVGVDLSDEAIAMARLHHPEVPYHAGDLFRTPIAGSPFDVVVSQEVIAHVADQT
                     EYLRRISGLLKPGGYLVITTPNKFVHVRAGFPAQPPEHIEQWLTRHALRRLLSAEFTV
                     LKMTTVNPLGAGGILRLVNSYKLNKVLELFVSDVTLRSVKERLGFGWTTAALARRRM"
     gene            complement(778..1428)
                     /locus_tag="VFZ80_03000"
     CDS             complement(778..1428)
                     /locus_tag="VFZ80_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019502660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX6286427.1"
                     /translation="MFRLGVQQVTPPLRSILCLGAHCDDIEIGCGGTILKLLDDRPDL
                     QVTWVVFSSDSVRRVEALKSAAAFLSAAHDVRVGEFRDGFLPSSSRELKEQFEELKNA
                     VSPDLVFTHYRHDLHQDHRLISDLTWQTFRDHLILEYEIPKYDGDLGSPNVFVHLDEA
                     VCRRKAATILSCFKTQTSKRWFAEDLFMALLRLRGMECNSETNYAEAFYCRKLVVA"
     gene            complement(1441..>2056)
                     /locus_tag="VFZ80_03005"
     CDS             complement(1441..>2056)
                     /locus_tag="VFZ80_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017286605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HEX6286428.1"
                     /translation="YDETVSNDFVLSQGGREIELRSRDIDDWRITFVDTGLHSNIGQR
                     LKAVEPYLEGEDSFLANYSDGLTDLHLPGYIENFRRQDKIASFLCVRPYHTFHVVTAD
                     DDGLVRDIRHIEQAGLWINGGFFAFKREVFDYLGEGEELVEAPFQRLIEKSELIAYKH
                     EGFWASMDTFKDKQIFDHMDARGDTPWAVWRCQPRQPSPQPPAF"
BASE COUNT          392 a          631 c          662 g          371 t
ORIGIN      
        1 cagacgatga cgctcacatc cttctccgcg ccagcgcagc ggtcgtccag ccgaagccca
       61 gccgttcctt cacggagcga agcgtgacat ccgacacgaa gagctccagc accttgttca
      121 gcttgtacga gttgacgagc cgcaggatgc ccccggcacc gaggggattg accgtcgtca
      181 tcttgagcac ggtgaactcg gcactcagga gtcgtcgaag cgcgtgtcgg gtcagccact
      241 gctcgatgtg ctcgggcggc tgggcaggaa atccggcccg aacgtgaacg aacttgttgg
      301 gcgtcgtgat gacgagataa ccgcccggct tgagcaggcc cgagatgcgc cgcaggtact
      361 ccgtctgatc agcaacgtgg gcgatgacct cctgggagac gacgacgtcg aacgggctcc
      421 cggcaatcgg cgtccggaag agatcgccgg cgtggtacgg gacctcggga tgatggagac
      481 gggccatggc gatggcctca tcggacagat cgacgccgac ggcctcgccc agctcggcga
      541 gccggggagt cagccagccg gtgccacagc cgagatccag gatcctcgga cgcgagaggc
      601 cgagctcggc gatcagatcg aggatcatct cgccgcgccg tcgagcgccc tcatgcgggt
      661 agctcatctg ttcccgttgc cacttcgtgt cccagtagcg cttctgtcca gtgaggtccg
      721 ggacaaatcc acttggcgtt gacacgttac ttccccttct gccggatcca acgatcgtca
      781 tgcgacgacc agcttgcgac agtagaacgc ttcagcgtag ttcgtctcgg agttgcactc
      841 catgccgcgc agccgcagaa gcgccatgaa gaggtcctcg gcaaaccatc gctttgatgt
      901 ctgtgtcttg aaacacgaga ggatggttgc tgccttccgg cggcagacgg cctcgtcgag
      961 atggacgaag acgttcgggg atcccagatc accgtcgtac ttgggaatct cgtactcgag
     1021 gatcaggtga tcccggaacg tctgccaggt gagatcggag atcagccggt gatcctggtg
     1081 gagatcatgg cggtagtgcg tgaagacgag atccggtgag acggcgttct tcagctcctc
     1141 gaactgctcc ttcagctccc tggagctcga gggcagaaag ccgtcgcgaa actccccgac
     1201 gcggacatcg tgagccgccg acaggaaggc ggcagcactc ttcagggctt ccacccgacg
     1261 cacactgtcc gagctgaaga cgacccaggt gacctgaaga tcgggccggt cgtcgaggag
     1321 cttcaggatg gtgccaccgc acccgatctc gatgtcgtcg caatgagcgc cgaggcagag
     1381 gatgctccgg aggggcgggg tgacctgctg tacgcctagc cgaaacatgg cgggaggtgg
     1441 tcagaaggct ggaggctgag gcgacggctg ccgaggctgg cacctccaca cagcccacgg
     1501 cgtgtcaccc cgggcgtcca tgtggtcgaa gatctgcttg tccttgaacg tatccatgga
     1561 ggcccagaag ccctcgtgct tgtacgcgat cagctcgctc ttctcgatga gacgctggaa
     1621 cggcgcctcg acgagctcct ctccctcacc gagatagtcg aagacctcgc gcttgaaggc
     1681 gaagaagcct ccgttgatcc acaagccggc ctgttcgatg tgccggatgt cgcgcacgag
     1741 gccgtcgtcg tccgcggtga cgacgtggaa cgtgtgatac ggccgcacgc acaggaagct
     1801 cgcgatcttg tcctgccggc ggaagttctc gatgtacccg ggcaagtgca gatcggtcag
     1861 gccgtcgctg tagttggcga ggaacgagtc ctcgccctcc aggtacggct cgaccgcctt
     1921 gagacgctgg ccgatgttgg agtgcaggcc ggtatcgacg aaggtgatcc tccagtcgtc
     1981 gatgtcgcgg ctccgcagct cgatctccct gccgccctgg ctcagaacaa aatcgttgga
     2041 cactgtttca tcgtaa
//
LOCUS       DASUTA010000149         2477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_176857, whole genome shotgun sequence.
ACCESSION   DASUTA010000149 DASUTA010000000
VERSION     DASUTA010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176857"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFZ80_03010"
     CDS             <1..204
                     /locus_tag="VFZ80_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX6286429.1"
                     /translation="MISEDKYCIDVLTQVNAASAALKGVAVGLLDEHVRHCVREAAEA
                     GDQRSADEMVTEATKAIERLVRS"
     gene            294..2315
                     /locus_tag="VFZ80_03015"
     CDS             294..2315
                     /locus_tag="VFZ80_03015"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017197946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEX6286430.1"
                     /translation="MSEDHGHDSQGHDAHGGHDKHAGHSVAMFRDRFWLSLALTIPVV
                     FYSEMIQEWFGYTAPTFPGSDWIAPVLGTVIFVYGGSPFLKGAVQEARDRQPGMMLLI
                     GMAITVAFLASAATTVGWFDLDFWWELAALITIMLLGHWMEMRAIGQARGALAALAEL
                     LPDEAELVTDSGTETVSLAELAVGDVVLVRPGGRIPADGEIMSGEVEVDESMVTGESN
                     PVAKKPGDEVIAGTVATDNSIRVAITAVGDETALAGIQRLVAQAQESRSRAQVLADRA
                     AALLFYVAVAAALITVVTWLIVDQPDQAVVRTVSVLVIACPHALGLAIPLVIAISTSM
                     AARNGILVKDRLALERMRNIDVVLFDKTGTLTKGSHRVSGIAAIDSDDDRLLALAGAV
                     ESASEHPIARAIVAAATEQGSVPTASDFKSMSGRGAQAVVDGVPIAVGGPALLAERDL
                     EVPTELEQASDDWRARGAAILYVVANDRVIGALSTEDEIRPESREAVDELHRLGVKVA
                     MITGDAHQVAEAVAAELDIDHVLAEVLPEDKHSKVAELQRQGSKVAMVGDGVNDAPAL
                     AQADVGIAIGAGTDVAIESAGIVLASDDPRGVVAVRKLSQAGYRKMIQNLVWATGYNA
                     AAIPLAAGALAWAGIVLGPAVAAVLMSASTVIVAINAQFLRRVDLRPGQ"
BASE COUNT          468 a          807 c          790 g          412 t
ORIGIN      
        1 atgatcagcg aggacaagta ctgcatcgac gtcctcactc aggtgaatgc ggcttcggcg
       61 gcgttgaagg gggtcgctgt cggtctgttg gacgagcacg ttcgccattg tgtccgtgag
      121 gccgccgagg ccggcgacca acgcagcgcc gatgaaatgg tgacagaggc gaccaaggcg
      181 attgaacggc tggtccgttc ctgaggggcg aagcgatgag cacacacgac catcaccacg
      241 acgaacacgg agcatctacc acgactcgga ctgacgagac gatgggtcac gacatgtcag
      301 aggatcacgg acacgactca caaggtcatg acgcacatgg tgggcatgac aagcatgccg
      361 gtcacagtgt ggccatgttc cgggatcggt tctggctgtc gcttgccctg accatccctg
      421 tcgtcttcta cagcgagatg atccaggagt ggttcggcta taccgcaccg acctttcccg
      481 gatcagactg gatcgctccc gtcctgggaa cggtcatctt cgtctacgga gggtcgccgt
      541 tcctgaaagg agcggtccag gaggcacgcg accgccagcc cggaatgatg cttctgatcg
      601 gcatggcgat caccgtcgcg ttcctcgcct cggcagccac cacggtgggc tggttcgacc
      661 tcgacttctg gtgggaactg gccgcattga tcacgatcat gctcctgggc cactggatgg
      721 agatgcgcgc catcggccag gcccgaggcg cactggctgc actcgccgag ctgttgcctg
      781 atgaagcgga actggtcacc gactccggca ccgaaacggt ttccctggct gagctcgcgg
      841 ttggggatgt cgtcctggtg cggcccggcg gacgcattcc agccgacggt gagatcatga
      901 gcggagaggt cgaggtcgac gagtcgatgg tcaccggtga gtccaatccg gtcgccaaga
      961 aacccggcga tgaagtaatc gcagggacgg ttgcaaccga caattcgatc cgcgtggcga
     1021 taacggcggt cggcgacgaa acggctctcg ccggtattca gcgtctcgtc gctcaggccc
     1081 aggaatcccg ttcccgggcc caggtcctcg ccgaccgggc ggcagcgctc ctcttctatg
     1141 ttgcggtggc ggcggcgctg atcaccgtcg tgacgtggct gatcgtggac cagccagatc
     1201 aagccgtagt caggacggtc tcggtcctcg tgatcgcctg tccccatgca ctcggtctag
     1261 ccatcccact tgtgatcgcc atctcgacgt cgatggcggc ccgcaatggg atcctggtca
     1321 aggatcggct cgccctggag cggatgcgca acatcgacgt ggtcctcttc gacaagaccg
     1381 gcaccctcac caaaggaagc cacagggtca gcgggatcgc cgcgatcgac tccgacgacg
     1441 accggctgct cgccctcgcc ggcgcagtcg aatcggcctc ggaacatccc atcgctaggg
     1501 ccatcgtcgc cgcagccacc gaacaaggat ccgtccccac cgcctcggac ttcaagtcga
     1561 tgagcgggcg aggagcccag gccgtcgtcg acggggtccc gattgctgtc gggggaccgg
     1621 cactgttggc cgagcgggat ctcgaggtcc cgaccgagtt ggagcaggcg agcgatgact
     1681 ggagggcccg tggcgcagcg atcctctacg tcgtcgcgaa cgaccgtgtg atcggagccc
     1741 tgtcgaccga agacgagatt cgacccgaat ccagagaagc cgtcgatgag ctgcaccgac
     1801 tcggggtcaa ggttgcgatg atcactggcg acgcccacca ggtggccgaa gcggtagcag
     1861 cggaactgga catcgaccat gtgctcgctg aagtcctccc cgaggacaag cactccaaag
     1921 tcgccgaact gcaacgacaa ggctcgaagg tcgcgatggt gggcgacggc gtcaacgacg
     1981 cccccgcact cgcccaagcc gacgtcggta tcgccatagg cgccggcacc gatgtcgcca
     2041 tcgaatcagc cggcatcgtc ctcgcctccg acgacccccg cggagtggtc gcagtccgaa
     2101 agctgtcaca ggcgggatac cgcaagatga tccagaacct ggtctgggcc accggataca
     2161 acgccgccgc aatccccctc gccgcaggag cgctcgcctg ggccggcatc gtcctcggcc
     2221 ctgccgtggc cgccgtgctg atgagcgctt ccaccgtgat tgtcgctatc aacgcccagt
     2281 tcctccgacg ggtcgaccta cgacccggcc aatgaccctg cgacgactgc tggctgcgct
     2341 caccctggtc atgatggttg ccgcgtgcgg cacgtccgaa ggaacggaga tcactccagc
     2401 cgacccggag ctggtggaga ccggagcaga cctatacgcc gccaactgcg cccaatgcca
     2461 cggaaccgac ctcaggg
//
LOCUS       DASUTA010000150         4726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1730163, whole genome shotgun sequence.
ACCESSION   DASUTA010000150 DASUTA010000000
VERSION     DASUTA010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730163"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VFZ80_03020"
     CDS             <1..786
                     /locus_tag="VFZ80_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX6286431.1"
                     /translation="AGYFVDAIVRRGETRQLVCASTGNFGQAMAYACRAHERDLVVFA
                     DTSANELKMSRIRDLGADVRLRGNDFDAAKEVARRFCVEHDAWMIEDGREPEISEGAG
                     TIGVELTRLRDYDAVVVPVGNGALISGVARWVKAVSPTTRVVGVSAVGADSMVESWRS
                     GQIVERPTVETIAEGIAVRIPVPEAVEDMIPIVDEMVVVSDQQMIEAMRAIHRLAGQM
                     IEPSGAAGVAAITSDLERFEGMSVATVLTGNNLTEEQIDRWLG"
     gene            complement(793..2397)
                     /locus_tag="VFZ80_03025"
     CDS             complement(793..2397)
                     /locus_tag="VFZ80_03025"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX6286432.1"
                     /translation="MQDDFPLLLSTLYDHAVWIHPDQEIVSVESDRSLSRWTYGDVDS
                     RVRRLASAFKTLGIGQGDAVGTFAWNNHRHHELYWATANSGRICHTINIRLFADQVVY
                     IVNHAGDKAIFVDPDLVPLIAPLVDQFEAVETIVIMGEHASDEIDGSIAYEDLIADQE
                     EHGKWPLLDERSAMMLCYSSGTTGHPKGVAYTQRSTYLHTISNLATLSIQPADNVLPV
                     VPMFHAAAWGYPFMATTKGAKLTYPGPDLSPEGLVKLFTGERVTFSAGVPTVWLGIKQ
                     YLDAHPEVDVSSINAFLCGGSAVPRSLIEAYWRDRGIRVIQGWGMTETNPIASMAVLK
                     PHMEDWDLEEQLDRLETAGIPTAGLQVKIVDEVGAELPHDGEAFGELLIRGPWIAAEY
                     YKDDRTPQSFEDGWLRTGDVCKITPEGYIRITDRAKDVIKSGGEWISSLDVENELMAH
                     PAVAEATVVGLKHVKWQERPAAFVVLAEGAEATPEELIEHLSSRVAKWWLPDEIVFID
                     EIPKTGTGKFDKKVVRDQYADLLMGD"
     gene            complement(2403..3005)
                     /locus_tag="VFZ80_03030"
     CDS             complement(2403..3005)
                     /locus_tag="VFZ80_03030"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX6286433.1"
                     /translation="MGYPERLLSDDEVIEAQFRPHWSGILREGLIILIGIALAVLVAI
                     FSWPAWLYAVIAGLVLILIVRGLARWLTTLHVVTNERLIYRAGFIAKHGTEIPLEVIQ
                     NVAFNQTIIERVFGTGDLMIESAGTHGQTRYKDIPKPEGVQSLIYKMREIRLREVERG
                     ETSSGSALSDLERLSRLHDQGKLSDAEFEAQKARLLGGDA"
     gene            3623..4081
                     /locus_tag="VFZ80_03035"
     CDS             3623..4081
                     /locus_tag="VFZ80_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286434.1"
                     /translation="MGALRDCEAVSDSLLGQPVNSLTTLAFMAAGVVVWRMTERHWVA
                     VGLMATGLGSFLFHGPMPSYAQLAHDVTLWFLVVVVVASIVVEFRSTGEWRHLVGPGL
                     LLAVVAIVGRLGATGNPLCDPDSLLQPHGLWHIGSATAVAWWALNHGKPG"
     gene            4373..>4726
                     /locus_tag="VFZ80_03040"
     CDS             4373..>4726
                     /locus_tag="VFZ80_03040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6286435.1"
                     /translation="MWSDRNVRAVVLARFISRVGGEAAFFVGIWGKAAFELDATPSEL
                     ALVMAALGLASLIGASVAGVLVDRFDPRRVVLAGEVLFVPAALALIFTDTIPSLAAAT
                     FVLGLVGTPVFTAISS"
BASE COUNT          842 a         1509 c         1438 g          937 t
ORIGIN      
        1 gccggttact tcgtcgacgc aatcgtcagg cgaggagaga cccggcagct cgtttgcgcc
       61 agcaccggca acttcggtca ggccatggcg tacgcatgcc gcgcacacga gagggacctc
      121 gtcgtcttcg cagacacctc tgccaacgag ctcaagatga gcaggatccg cgatctcggc
      181 gccgacgttc gtctccgcgg caacgacttc gatgcagcca aggaggtggc tcggcgattc
      241 tgcgtcgaac acgacgcctg gatgatcgaa gacgggcgag agccggagat cagcgagggt
      301 gcaggcacta tcggggtcga actcaccaga ctgcgcgatt acgacgcggt cgtcgtcccg
      361 gtcggcaacg gcgccctgat ctcaggagtg gcacgttggg tcaaagcggt ctcaccaacc
      421 actcgggtgg tcggagtctc ggcggtgggc gccgactcca tggtcgagtc atggcgcagc
      481 gggcaaatcg tggagcggcc gacggtggag accatcgccg aaggaatcgc ggttcgcata
      541 cccgtgcccg aagccgtcga ggacatgatt cccatcgttg acgagatggt tgtcgtgagc
      601 gaccagcaga tgatcgaggc gatgagggcg attcacaggc tggcaggcca aatgatcgag
      661 ccatcgggtg cagccggagt cgccgccatc acctccgatc tcgagagatt cgagggcatg
      721 agcgtggcca ccgtgttgac cgggaacaac ctcaccgagg agcagatcga ccgctggctc
      781 gggtgatcag actcaatctc ccatcaggag atcggcgtat tggtcccgaa ccaccttctt
      841 gtcgaacttc cccgtcccgg tcttgggtat ctcgtcgatg aagacgatct cgtcgggcag
      901 ccaccacttg gcgacacgtg acgacagatg ctcgatcaac tcctcggggg tggcctccgc
      961 accctccgcc aacaccacga aggccgccgg gcgctcctgc catttgacgt gcttcagacc
     1021 gaccaccgtc gcctcggcga cagcagggtg tgccatcagc tcgttctcga cgtccagaga
     1081 gctgatccac tccccacccg acttgatcac gtctttggca cgatcagtta tccggatgta
     1141 gccctccggg gtgatcttgc acacgtctcc ggtgcggagc cagccgtctt cgaacgattg
     1201 gggagtgcga tcgtccttgt agtactcggc cgcgatccag gggccacgga tcaatagctc
     1261 tccgaatgcc tcaccgtcgt gtgggagctc ggccccgacc tcgtcgacga tcttgacttg
     1321 gagaccggcc gtaggaatgc cggcggtctc cagacgatcc agctgctcct cgaggtccca
     1381 gtcctccatg tgtggcttca gcactgccat cgaagcgatt gggttggtct cggtcatgcc
     1441 ccacccctgg atcacccgga tcccgcgatc tctccaataa gcctcgatca atgagcgcgg
     1501 aaccgccgag ccgccacaga gaaaggcgtt gatcgaggag acatcgactt cggggtgggc
     1561 atctaggtac tgcttgattc ccagccagac cgtcggcacc ccggcgctga acgtgactct
     1621 ctctccggtg aacagcttca ccagcccttc cggtgagagg tccggacccg gataggtgag
     1681 cttggctccc ttggtggtgg ccatgaaggg gtatccccac gccgcagcgt ggaacatagg
     1741 gacgaccggc agcacgttgt ccgcgggctg gatggacaat gtcgccaggt tcgagatcgt
     1801 gtgcaggtag gtcgatcgct gcgtatatgc gacgcctttg gggtggccgg tggtccccga
     1861 cgagtaacag agcatcatgg ccgaccgctc gtcgaggagg ggccatttcc cgtgctcttc
     1921 ctggtcggcg atcaggtctt cgtaagcaat cgagccgtcg atctcatcac tggcatgctc
     1981 ccccatgatc acaatggtct ccaccgcctc gaactggtcc acgagcggtg cgatcagagg
     2041 aaccagatcg gggtccacga agatcgcctt gtctccagca tggttgacga tgtagacgac
     2101 ctgatcggcg aacagccgga tgttgatcgt gtgacagatc cgcccgctgt tcgctgtcgc
     2161 ccaatagagc tcgtgatgac ggtggttgtt ccaggcgaag gtgcccaccg cgtctccctg
     2221 ccctatgcca agtgtcttga acgccgatgc cagccgtcgc acccgggagt cgacatcgcc
     2281 gtaggtccac cgggagagcg agcggtcgct ttcgaccgag acgatctcct gatcgggatg
     2341 tatccacacc gcatggtcgt acagcgtcga cagcagcagc gggaagtcgt cttgcatgtg
     2401 catcaagcat cacctccgag cagtctcgcc ttctgagctt cgaactccgc gtccgatagc
     2461 ttgccttgat cgtggagcct ggatagtcgc tccagatcgg agagcgccga tccggaggag
     2521 gtctcaccac gctcgacttc gcgcagccgg atctcacgca tcttgtagat cagtgattga
     2581 acaccctccg gcttggggat gtccttgtag cgcgtctgac cgtgtgttcc ggccgactcg
     2641 atcatgaggt cacccgtgcc gaaaacgcgc tcgatgatcg tctggttgaa ggcgacgttc
     2701 tggatgacct ccaatgggat ctcggttccg tgcttggcga tgaaaccggc ccgatagatg
     2761 agacgctcgt tggtgacgac gtggagagtg gtcagccagc gggccaaacc ccggacgatg
     2821 aggatgagca cgagaccggc tatcactgcg tatagccacg ccggccacga gaagatcgcc
     2881 accagaacgg ccagggcgat cccgatcagg atgatgaggc cctctctgag gatcccggac
     2941 caatgcggtc gaaactgggc ctcgatgacc tcgtcatcac tcagcagtcg ttcgggataa
     3001 cccattttcg cctcggctcc tcgacttgaa ctgcacggag gataacgagg tccatctacc
     3061 tgttatcgga ccagatacga gatggccccc ggaacgcgag aaaccccgag ggagccgaaa
     3121 gccgaagcct caaccctctc ggggctctct tacccaatcg gtgtcgggcc ggggacccaa
     3181 ctccgtttgg gagtcgcagt gggcgaaccc actgctgtgt gactcgtgag agtcacatgg
     3241 cactggtctc acgacctggt ctccgctcga tctctcgatc gctcgtgacc gaccctctcg
     3301 ggtctgccta tgacagacct ctcaatctgt ccgggtcttc accgctcgac gatcccgaag
     3361 gatccccgtc cgggctccga cccaaccttc cgcctcagtt tcccgagact tccagccgac
     3421 cctcgaaccg gcaaagccgg ccctccggtc cgacctgttt ggcggttacc cgccgtttcg
     3481 gtcgccgtcg ccgagtttcc ccgtcgacgt ggcgggaaaa gtagaccagc cccccaaccc
     3541 tggcaagtcc agagcctcat tgtttttcgc gagctgtaaa tcggttttcg cgtatcagtg
     3601 acaagccgta ttttctctgt tcatgggagc cctgcgcgac tgcgaggccg tttccgactc
     3661 gctgctcgga cagccggtca attcgctgac gaccctcgcg ttcatggctg ccggcgtagt
     3721 ggtctggcgt atgaccgaac ggcactgggt agccgttggc ctcatggcca cagggctcgg
     3781 ctcgtttctc tttcacggcc cgatgccgtc ttacgcccaa ttggcccacg acgtcactct
     3841 gtggtttctg gtggtcgtcg tcgtagcctc gatcgtcgtc gagttccgct cgactggaga
     3901 gtggcggcat ctggtcgggc caggcctgct tctcgcagtg gtcgccatcg tggggcgact
     3961 gggtgcaacc ggaaacccgc tgtgtgaccc cgactcattg ctgcagccgc acgggctgtg
     4021 gcacatcggc tcggccacgg ccgtggcctg gtgggccctc aaccacggaa aacccggttg
     4081 acctagaacg cgagaaaccc cgagaaagcc gaaggccgaa gcctcagccc tctcggggct
     4141 ctcaggtgtc actccacccg gaaagcgggc cccgggatgg ggtgacgtcc agagagaccg
     4201 aagtctctcc ttccccgatc gaggttgccc tcatccggta cggggcaacg tacgcagggc
     4261 ccaaatctcc gtcaaggccg attcggtctt tgttttcgcg tcttacgcct agttttcgcg
     4321 ctgagaatta ggtccgaagc cgtcccctca cggcggtagc gtcaacgccg cgatgtggtc
     4381 agatcgaaac gtccgtgccg ttgttctcgc gcgcttcatt tcccgggtgg ggggcgaggc
     4441 cgccttcttc gtcggcatct ggggcaaggc cgcgttcgag ctcgatgcga caccttcaga
     4501 gctggcgctg gtgatggcgg ccctcggcct cgcctccttg atcggtgcct cggtggccgg
     4561 tgtgctggtc gaccgattcg acccgagacg cgttgtgttg gcaggcgagg tcctcttcgt
     4621 gcctgctgcc ctcgccttga tcttcaccga cacgatcccg tcattggcag ccgccacatt
     4681 cgtcctcggc ctggtgggga cacccgtgtt cacggccatc tcctct
//
LOCUS       DASUTA010000151         4263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_694426, whole genome shotgun sequence.
ACCESSION   DASUTA010000151 DASUTA010000000
VERSION     DASUTA010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694426"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1648
                     /locus_tag="VFZ80_03045"
     CDS             <1..1648
                     /locus_tag="VFZ80_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6286436.1"
                     /translation="VLGTRDATPLEFWVGVGEGEYLQLDDVVALERVLPTGEVVRMYG
                     TVGQVVARHEGARFDSDVFLIEEGILPAEVTEAAQVTATRFEPEVFVPPRPGQEVRIA
                     EGDERDRALFFDRMERKVPVGLARNGEPLWVNFEFINGERGAHVNISGISGVATKTSY
                     ATFLLHSLFTSDVLGNAAVNTKALVFNVKGEDLMHLDRVNNRLDDEQRKRYADLGLPS
                     TPFDSVGLFAPPRLDDSKATADVASRSDVTSFFWSIHEFCIDDLLPFLFADAEDDRAQ
                     YTTVVYNVMAQLRHAEPTETGAAVIEGVKVRNFRELADLITERVQSDEHRNRWAGPAI
                     GTGTINAFVRRLQASVRHLGRLIRSDVPDPERHRISLQHQVTVVDLHNLNDRAKRFVV
                     GVTLKRAFEDKERAGQSDELLMVVLDELNKYAPREGSSPIKEVLLDVAERGRSLGVIL
                     IGAQQTASEVERRIIANSSIRVVGRLDSAEASREEYGFLPGPHRQRATIVKPGTMILN
                     QPELPVPLVLEFPFPAWATRASEAVPATDYSGPEDPFRDL"
     gene            1888..2472
                     /locus_tag="VFZ80_03050"
     CDS             1888..2472
                     /locus_tag="VFZ80_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6286437.1"
                     /translation="MRSLRYSINVTLDGCCHHEAGLPPDEESMRYWTAEMERADALLF
                     GRVTYQMMESAWRRPATGMWPDWMDEWEVPFAESIDRARKYVVSSTLSGVDWNAELVQ
                     GALGQAVQLLKEEPGEGLWVGGVTLPLALADLGLIDEYEFLVQPVLAGHGPTLLAGLR
                     ERIELELVDRHEFRSGSVALRYRPSESSSADQHS"
     gene            complement(2563..3615)
                     /locus_tag="VFZ80_03055"
     CDS             complement(2563..3615)
                     /locus_tag="VFZ80_03055"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX6286438.1"
                     /translation="MPVTDGLDQLESRFRIWERERHLSGGVLVTQAGSTLFEGSYGFA
                     DRSSGSPVTPRTRFGLASFTKMFTAVVVCDLVAVAALKFDTAIVDVLPPDRRPSTLDS
                     QVTVHHLLTHTSGIADYAEEAEDSPRYLADYGELWVDRPSYRMERPGDFLPLFSDLEP
                     YRPPGQRWQYSNAGFIMLGLVIEEITGRPYTEVVQERVFDRAGMGASGFFRLDEARAD
                     VAVGYLPDRSPDGHWRSNIYMIPVIGGADGGAFSTTHDLDQFLRCYADGTLLGPVQTV
                     ALTRHADAGHGFHSGYGVLHYPDASYGHGGGDPGVEVLVRRFPDQDAHVIVLCNGEGL
                     AGEVRDSILEALRFET"
     gene            3870..>4263
                     /locus_tag="VFZ80_03060"
     CDS             3870..>4263
                     /locus_tag="VFZ80_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286439.1"
                     /translation="MGDTRIRVVPAGEAGWGAVESVFRTDASSRNCWCQFHVLDNREQ
                     GSTTRDSRRRLLREQINTLDPPRGLVAFAADEAVGWCGVEPRTRLRHVLASRLVTANS
                     LHAADNPDVWAVYCILVPSSLRQKGIATT"
BASE COUNT          797 a         1314 c         1374 g          778 t
ORIGIN      
        1 agttctcggc acccgtgacg caacaccact cgaattctgg gtgggtgtcg gcgagggcga
       61 gtacctgcag ctcgatgatg tcgtggccct cgaacgggtg ttgcccaccg gtgaggtcgt
      121 ccgcatgtat gggaccgtgg gccaggtggt cgctcgccac gaaggtgcca ggttcgactc
      181 cgacgtcttc ctcatcgagg aagggatcct gccggccgag gtcaccgagg ccgcccaggt
      241 gaccgctact cgcttcgagc cggaggtatt cgtgcctccc cggcccggcc aggaggtccg
      301 catcgccgaa ggcgacgagc gagaccgtgc tctcttcttc gaccgcatgg agcgcaaggt
      361 cccggttggc ctcgcccgca atggcgagcc gctgtgggtc aacttcgaat tcatcaatgg
      421 agagcgtggc gcacacgtca acatctccgg catatcgggt gtggcaacaa agaccagcta
      481 tgccaccttc ctgctccact cacttttcac ctccgacgtg ttgggaaatg ctgcggtcaa
      541 cacgaaggca ctggtcttca atgtcaaggg cgaagacctg atgcatctcg accgggtcaa
      601 caaccggctc gacgatgaac agcgcaagcg atacgccgac ctgggtctgc cgagcacacc
      661 gttcgactcg gtcggtttgt tcgccccgcc ccgactcgac gacagcaagg ccacggctga
      721 tgtcgcctcc cgctccgatg tcacctcttt cttctggagc atccacgagt tctgcatcga
      781 tgaccttctc ccgtttctct tcgccgacgc cgaggacgat cgcgctcaat acacgaccgt
      841 tgtctacaac gtcatggcgc aactgcgaca tgccgagccc accgagaccg gggctgcggt
      901 catcgagggg gtgaaggtgc gcaacttccg tgagctggcc gatctcatca cagagcgtgt
      961 ccagagcgac gagcaccgca accgctgggc cggcccggcc atcgggaccg gcacgatcaa
     1021 cgccttcgtg cggcggctgc aggcttcggt tcgtcacctg gggaggctga ttcgttccga
     1081 cgtccccgac cccgagcgcc accgcatcag tctccagcac caggtgacgg tcgtcgatct
     1141 ccacaatctc aacgaccggg ccaagcgttt cgtggtcggg gtcaccctca aacgggcatt
     1201 cgaggacaag gagcgggcag gtcagagcga cgagctgctc atggtcgttc tagacgagct
     1261 caacaagtac gccccccgtg aaggctccag cccgatcaag gaagtcctcc tcgatgtcgc
     1321 cgagcggggc cgttcgctcg gggtgatcct gatcggcgcc caacagacgg cttctgaggt
     1381 ggagaggagg atcatcgcca actcatcgat ccgcgtggta ggtcggctcg actctgcgga
     1441 ggcgtcgcgt gaggaatacg gcttcctgcc cgggccccac cgccagcggg cgaccatcgt
     1501 caagccagga accatgatcc tcaatcagcc ggagctgccg gtgccattgg ttctcgagtt
     1561 tcccttcccg gcttgggcga cgagggcgtc ggaggcggtc ccggcgaccg actactccgg
     1621 gcccgaagac ccgttccggg atctatagac ggtttcagtc acccacccgg gcgacctagg
     1681 cgaccgtcgt atcgctgtat tcggcctcga tctcccagca gccttcttcg ggaatctgaa
     1741 acccgaccac caggaacacc ccttagccat catctcccaa aggcagccga cagtatctcg
     1801 cgctcggcct gtcgtttggt tcgcaccaca acggtgttga gtgggtacgg tccggaacgc
     1861 gcggcgcgca tcaaggagag caacgagatg agatcacttc gatactcgat caacgtcact
     1921 cttgatgggt gctgccatca cgaagcaggg ctgcccccgg acgaggagtc gatgcgttac
     1981 tggaccgctg agatggaacg agccgatgcc ctgctattcg gccgggtgac gtaccagatg
     2041 atggagtcgg cgtggcggcg gccggccacg ggcatgtggc ctgactggat ggatgagtgg
     2101 gaggtcccgt tcgccgagag catcgaccgg gcgaggaagt acgtcgtgtc gagcacgctg
     2161 agcggggtcg attggaatgc ggagctggtg caaggcgctt tggggcaagc ggttcagctg
     2221 ctcaaggagg agccagggga gggcctgtgg gtgggtggcg tgacgctccc cctggcgttg
     2281 gcagatctgg gactgatcga cgagtacgag ttccttgtgc agccggtgct tgccggacac
     2341 gggccgacgc tgctcgccgg tctgcgcgaa cgcatcgagc tcgagcttgt ggatcgccac
     2401 gagttccggt cgggctcggt tgccctgcgt tatcgcccaa gcgagtccag cagcgcagat
     2461 caacattcct gagcgcatag ctgagcgtct cacgtagtgc gcgatagcgg cacctcctga
     2521 gcgatttccg catcgaagga gcccttgctt gctgttcgcg gttcatgtct caaaccgcag
     2581 tgcctcgagg atcgagtcgc gcacttcgcc agccaagccc tcgccgttgc acagcacgat
     2641 cacgtgtgca tcttgatcgg gaaagcggcg aacgagtacc tcgacgcccg ggtcgccgcc
     2701 gccgtggccg taactggcgt cgggatagtg gaggacgccg tagccgctgt ggaagccgtg
     2761 cccggcgtcg gcatgtcggg ttagggcgac ggtctggacc ggccccagca gggtcccgtc
     2821 ggcgtagcat cgtaggaact ggtcgagatc gtgagtcgtg ctgaaggcgc cgccatctgc
     2881 accaccgatc accgggatca tgtagatgtt cgatcgccag tggccgtcgg gtgaccggtc
     2941 cgggaggtag ccgacagcta cgtcggctct ggcctcgtcc agccggaaga atccgctggc
     3001 ccccataccg gcccggtcga agactcgttc ttgcacgact tctgtgtacg gccggccggt
     3061 gatctcctcg ataacgagcc cgagcatgat gaagccggca ttggagtact gccatcgctg
     3121 ccctggtggc cggtaaggct cgaggtcgga aaagagcggc aagaagtctc ccgggcgttc
     3181 catgcggtag ctcgggcgat ccacccagag ctctccgtag tccgccaggt agcggggcga
     3241 gtcttctgcc tcctcggcgt agtccgcaat ccccgacgtg tgggtgagga ggtgatggac
     3301 cgtgacttgg gaatcgagtg tcgaaggtcg gcgatcgggg ggcaaaacgt ccacgatggc
     3361 ggtgtcgaac ttcaaggcag cgaccgcaac gagatcgcag accacgacag ctgtgaacat
     3421 cttggtgaac gaagccagcc caaaccgcgt gcggggggtg acaggagaac cgcttgatcg
     3481 gtcggcgaaa ccgtagctgc cttcgaacaa tgtcgaacca gcctgggtta ccaagacccc
     3541 tcccgacaag tggcgctcac gctcccagat tcggaagcgg gattcgagtt gatcgagacc
     3601 gtcagttaca ggcacatgcc cagagtgaca tcacttcccc gccccgacaa gcttctgacc
     3661 gtaacccggt cacggatcga gatccaagga tccgaaccgg tccgcgcgac cagcgtcaca
     3721 gaccgaagat gagcaggatt cgccagccga ggttttcggt ttcttgcggt gtggccccga
     3781 tggagcgtgg tcggacgagt ctctgtccaa atacgacgat gccagaaagg cggaagtggg
     3841 ctgtccggat tggaccttac cttggaggag tgggcgacac tcggatacgc gttgtgccgg
     3901 caggcgaagc cggttggggg gccgttgagt cagtgttcag aacggatgca tcgagccgca
     3961 actgctggtg tcagttccat gtcctcgata accgggaaca aggctcgacc acgcgggatt
     4021 cgagacgaag actgctgcgc gagcagatca acactctcga cccaccacgg ggtttggtgg
     4081 ctttcgccgc ggatgaagca gtcgggtggt gtggtgtcga gccacggacc cgcctgagac
     4141 atgtgctcgc cagtcggttg gtcacggcca acagcctcca cgcagccgac aatccagatg
     4201 tatgggcggt ctattgcatc cttgtgcctt cgtcgttgcg tcaaaagggc atcgcaacaa
     4261 ccc
//
LOCUS       DASUTA010000152         2660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1083597, whole genome shotgun sequence.
ACCESSION   DASUTA010000152 DASUTA010000000
VERSION     DASUTA010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083597"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VFZ80_03065"
     CDS             complement(<1..381)
                     /locus_tag="VFZ80_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286440.1"
                     /translation="MGDVDWKAIDAWRRDAVLAQPVESGSGAVEFLDLAYHSVGEPHS
                     ELRSLARVQRVDTDVAVHVGDVFPPVVHRLEITIGKCIGILVPLPLVEPNTETTVGCD
                     LVFTQGASRAASTSLSTVRVSCALK"
     gene            complement(410..1237)
                     /locus_tag="VFZ80_03070"
     CDS             complement(410..1237)
                     /locus_tag="VFZ80_03070"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6286441.1"
                     /translation="MSRVRRVDIGGLHIAVSEIGDGHPILLHPGLGYASWCWQQLAIL
                     LADRYRVISIDPRGAGQSDKPVGPYSIEQLADDVATLLDVTDEEPAHVIGHSMGGYVA
                     QMLAVRHPDAIRSLVLLSSSPGGPKSHRVPEGTIEVWRRAAGLPPAMFARETFPYSFR
                     LGWPEEHVSQYEGYLRDRLRWPTPPERWQDQYEACEVFLDSGIGRSVIGQPTLIIHGA
                     VDRVVPVANAYLLAGMIPNSSLLVLPNAGHNLMLEEPAFVAGRVSDFLEGPGEATWD"
     gene            complement(1234..2361)
                     /locus_tag="VFZ80_03075"
     CDS             complement(1234..2361)
                     /locus_tag="VFZ80_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6286442.1"
                     /translation="MKTRAAILDKVGHRPPFGESVPLTVEEIDLADPGPGEVLISVTA
                     AGVCHSDLAVVDGTRPWPMPMVLGHEAAGVVLGLGVGVESVSEGDQVVLSFVPACGIC
                     PTCVEGRPVLCPSAQIANGEGRLTTGRRPFKRNGAAVNTHLGVGAFAERTVVSELSVI
                     TLPGDVPPQVAAVMGCAVVTGVGAVLNTAGVREGQSVAVFGLGGVGLAVVMGAVLAGA
                     NPIIAIDAVGAKLDRARHLGATGTVDASSVDAVEAVRDLSGGGVDFAFEAVGSADVLK
                     QCYLAAGSGGSAVSIGLSPRQSEITINALSLVGLEKTLRGSYMGSARPRADIPRYVDL
                     WRAGLLPVDELITRSAIPIDDVNDALDRLAEGQEIRQIIEP"
     gene            complement(2405..>2660)
                     /locus_tag="VFZ80_03080"
     CDS             complement(2405..>2660)
                     /locus_tag="VFZ80_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6286443.1"
                     /translation="MPSYVGYRYFNTLLTVDLRDRLGEVQVPTLIVHGRHDQVCDPRW
                     AEYMAERISDAKLVWLDESGHASMVEQPHELSAEIASFVN"
BASE COUNT          508 a          882 c          819 g          451 t
ORIGIN      
        1 tttcaaggcg caagagaccc tgaccgtcga cagagaggtg cttgcagctc tcgaggcacc
       61 ctgagtgaat acgagatcac acccgacggt ggtctccgtg ttcggttcca cgagcgggag
      121 tggcacgagg atcccgatgc atttgccgat ggttatctca agtcggtgaa ccaccggtgg
      181 gaagacatcc ccgacgtgca cggcgacatc cgtatctaca cgctggactc tggcgagcga
      241 gcggagttcc gagtgcggtt caccgacgga gtggtaagcc aggtcaagga actcgaccgc
      301 gccgctccct gactccactg gttgagccag aacggcgtcc cgccgccaag cgtcgatcgc
      361 cttccagtcg acgtcgccca atgagcccaa tgagcaggca ggttcccgat cagtcccatg
      421 tggcttcacc cgggccttcc aggaagtcgc tgactctgcc tgcgacgaac gccggctctt
      481 ccaacatgag gttgtgcccg gcgttcggga gtaccaagag tgagctgttc gggatcatgc
      541 ccgccagaag gtaggcattg gccacaggaa caacccgatc gacggcaccg tggattatga
      601 gagttggctg accgatcacc gaacggccga tcccagagtc gaggaagacc tcacacgctt
      661 cgtattggtc ttgccaccgc tctggaggtg tcggccagcg aagacgatct ctcaggtagc
      721 cctcgtattg ggagacgtgt tcttcgggcc agcccaaacg gaacgagtag gggaaggtct
      781 cgcgtgcgaa catcgcggga ggtagtcccg ccgcccgtcg ccagacttcg attgtgccct
      841 cggggacgcg atgagacttg ggaccgccgg gcgaactgga gagcaacacc aggctcctga
      901 tcgcgtccgg gtgcctcacc gcgagcatct gcgcgacata gccacccatg gaatgaccga
      961 taacgtgggc aggctcttca tcggtgacgt cgagcagagt ggccacatcg tcggcgagct
     1021 gctcgatgga atagggcccg accggcttgt cggattggcc ggcaccgcga gggtcgatgc
     1081 tgatcacccg ataccggtcg gccaagagga tcgctagctg ctgccagcac cacgacgcgt
     1141 agccgaggcc gggatggagg aggatcggat ggccgtctcc gatctcgctc acggctatgt
     1201 gaagtcctcc gatatcgact cttcggaccc ggctcacggc tcgatgatct ggcggatctc
     1261 ctgtccctct gccagacggt ccaaggcatc gttcacatcg tcgatcggga tggcggaccg
     1321 cgttatcaac tcatcgaccg gcagaagccc ggcacgccac aggtcgacat atcgagggat
     1381 gtccgcgcgt ggacgcgccg agcccatgta ggagcctcgc agcgtcttct ccaggcctac
     1441 gaggctcagc gcgttgatcg tgatttcgct ttgacggggg gaaagcccga tcgacacagc
     1501 actgccaccg gagccagctg cgagatagca ctgtttcaac acatccgccg acccgaccgc
     1561 ctcgaacgcg aagtccactc caccaccgct caggtcacgg acggcttcga cggcgtcgac
     1621 cgaggacgcg tccaccgtgc ccgtagcacc gaggtgacgg gctcggtcca gcttcgctcc
     1681 aaccgcgtcg atggcgatga tcggattcgc ccccgccaag accgctccca tgacgacggc
     1741 gagcccgact cccccgagtc cgaacaccgc cacggactgg ccctctcgca cccctgccgt
     1801 gttgaggacg gcccccaccc ccgtgaccac ggcgcaaccc atgacagcag ccacctgcgg
     1861 tgggacgtcc cctggaagcg tgatcaccga gagttccgag acgacggttc tctccgcaaa
     1921 ggcaccaacg ccgaggtggg tgttgacggc ggcgccattg cgtttgaacg gcctgcgccc
     1981 tgtggtcaga cgcccttcgc cgttggcgat ctgtgcgctg gggcacagga ccggcctccc
     2041 ctcgacgcag gtggggcata tcccgcaggc aggcacgaag ctcagcacca cctgatctcc
     2101 ctcggagacc gactccaccc ctacaccgag tcccaacacc acacctgccg cctcgtggcc
     2161 cagaaccatt ggcatcggcc aaggccgggt cccgtcgacc actgccaggt cggagtggca
     2221 cacgcccgcc gcagtcacgg agatcaggac ctcgcccggg ccgggatcgg cgagatcgat
     2281 ctcctcgacg gtcagcggca cggattcacc gaaaggtgga cgatggccga ccttgtccag
     2341 gatcgcggct cgtgtcttca tccctttgcg cctcggcagt tgtcggagcc gaagcgatcg
     2401 tcattcaatt aacgaagctg gcgatctcgg ccgacagctc atggggttgc tcgaccatag
     2461 aggcatgccc gctctcgtct agccacacga gcttggcgtc gctgatccgc tcggccatgt
     2521 actcggccca tcgcgggtcg caaacctggt catgacgccc gtgcacgatg agcgtcggaa
     2581 cctgtacctc tccgaggcga tccctcaagt cgaccgtgag gagggtgttg aagtagcgat
     2641 acccgacata ggagggcatc
//
LOCUS       DASUTA010000153         3562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_694572, whole genome shotgun sequence.
ACCESSION   DASUTA010000153 DASUTA010000000
VERSION     DASUTA010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694572"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1927
                     /locus_tag="VFZ80_03085"
     CDS             <1..1927
                     /locus_tag="VFZ80_03085"
                     /inference="COORDINATES: protein motif:HMM:NF033681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ExeM/NucH family extracellular endonuclease"
                     /protein_id="HEX6286444.1"
                     /translation="VPGQVYDCSEEGWLIGDFTPGLDDTPGEVNICDAPSVEPTPHLI
                     SEIQGEGQASPHQDELVSIEGVVVGDFQEGDGDHTDLGGFFVQEETDDQDANPNTSEG
                     VFVFAPGAIDVSEGDVVEVIGTVDEFFGRTQVADVVEVEILGSGSVPATQVTLPVTAT
                     SDLEKYEGMLVTFPQALVISEFFNFDRFGEIVLTTDRQYQGTHVAEPGGDANAVTAAN
                     RLSRITLDDGRDSQNPDPAIHPNGQEFTLTNTFRGGDTLENVTGAMDWSFGLYRIQPT
                     QGADYLNANPRPTSPDPIPGDVRVASFNVLNFFTHLDDGVNDICGPTATMECRGADTA
                     EELDRQLAKLVSGILALDADIVGVQEIENDIRDDEPVFPNRAHDAVLTLVEALNEVEG
                     STAWAWVGEASHYNDYPVRNDIIYRKSAVSPIGDPVALADMAFDRTRPETPDEPLGRP
                     PLAQTFRTHGPGAQSAFTVVVNHFKSKGSDCASIGDPDLGDGQGFCNLTRVAQSEALL
                     DFVDELSEDSPGGVLVIGDLNSYRNEDPIDTLEGGGLTDLVESFVGEDAYSYVFDGQL
                     GYLDTALANDTMSDNVQGVTIFHINADEPDILDYDMSFKQDAQDALFEPLPYRVSDHD
                     PVIVGLTIPRPGRGPDS"
     gene            complement(1967..2983)
                     /locus_tag="VFZ80_03090"
     CDS             complement(1967..2983)
                     /locus_tag="VFZ80_03090"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019825919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX6286445.1"
                     /translation="MPTLTLSTTIAGSLPKPSWLASRDQLWAGWVQEGEDLAEAKRDA
                     VRVAVLEQERAGLQIVTDGEQTRQHFVTTFIEGLEGVDAVNRATVQIRRRYDASVPRV
                     VGEVSRRGGVFVDETRFLRSLTDLRIKTQLPGPMTMADTCQDDHYGNRETLAMSFAEA
                     LNAEAREMAEAGADIIQFDEPAFNVFLDEVGGWGIEALEKAIEGVDAITAVHICYGYG
                     VKANIDWKRGLGPEWRQYEVTFPVLADSSIDQVSLEMAGSHVPLEVLDLLGDKSVQAG
                     VIDVATDEVESPQDVASLLESVAGHVPLERIYPSTNCGMVPLKRSLAHAKMEALAAGA
                     ALLE"
     gene            complement(3002..>3562)
                     /locus_tag="VFZ80_03095"
     CDS             complement(3002..>3562)
                     /locus_tag="VFZ80_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286446.1"
                     /translation="LNEVLASSGGAGPEVNSSLGIAMVARLAARHGIDVRLVPSVPGT
                     TARVTVPASLVSTVGGLEGDDQAEPPPESSYRSSHVVAMTESARREAEAFLEKVFGPL
                     RGRSATRRPAKSNGNGHVAPVMPSQEGPGLQTRVPGTNFAVTEDDPSVVSGEGAIDIR
                     LNLSRYDEGRRQAADPELDREGDTDS"
BASE COUNT          678 a         1148 c         1109 g          627 t
ORIGIN      
        1 cgtgcccgga caggtctacg actgcagcga agaaggatgg ctcatcggcg acttcacacc
       61 cggtctggac gacacgccgg gcgaggtgaa catctgcgac gcaccatcgg tcgaaccaac
      121 gccccacctg atcagcgaga tccagggcga gggccaggcc agcccccacc aagacgagtt
      181 ggtctcgatc gaaggagtag tggttggaga cttccaggag ggcgacggag accacacaga
      241 tctcggcggt ttcttcgttc aggaggagac ggatgaccag gatgccaacc caaacacgtc
      301 ggaaggggtg ttcgtcttcg caccaggcgc gatcgatgtc agcgagggtg acgtcgtcga
      361 agtcatcgga accgtggatg agttcttcgg gagaactcag gtggctgacg tcgtggaggt
      421 ggagatcctg ggctcgggaa gcgtcccggc cacgcaggtg actctgcccg tgacagcgac
      481 atcggacctg gagaagtacg aggggatgct cgtcaccttc ccgcaggctc tggtgatctc
      541 cgagttcttc aatttcgacc gtttcggaga gatcgtcctc accacagacc ggcaatacca
      601 aggcacacac gtggccgagc ccggtggcga cgccaatgcc gtgacggcgg ccaaccgtct
      661 ctcccggatc accctcgacg acggacggga cagtcagaac ccggatccgg caattcaccc
      721 caacggccag gaattcacct tgaccaacac attccgaggc ggtgacacgc tggagaacgt
      781 caccggcgcc atggactggt cattcggcct ctatcgcatt cagccaaccc agggtgccga
      841 ctatctgaac gccaacccga gacctacgtc tccggatcca attccgggag atgtccgggt
      901 cgcctcgttc aacgtcttga acttcttcac ccacctcgat gacggagtca acgacatctg
      961 cggaccgaca gccacgatgg agtgtcgcgg tgcggacacg gcagaggagc tcgatcgtca
     1021 gctggccaag ctggtgtccg gcatcctcgc tctcgatgcc gacatcgtcg gagtccagga
     1081 gatcgagaac gacattcggg acgacgagcc ggtcttcccc aacagggcgc acgatgccgt
     1141 gctgactctc gtggaggcac tcaacgaggt ggagggatcc accgcgtggg cctgggtggg
     1201 tgaggccagc cattacaacg actacccggt gcgcaacgac atcatctacc ggaagtcagc
     1261 ggtgtctccg ataggtgacc ctgtggctct ggccgacatg gcctttgacc gaactcgtcc
     1321 agagacgccg gacgaacccc tgggacgacc tcctttggcg cagactttca ggacgcacgg
     1381 tccaggtgcc cagtcggcgt tcacggtggt ggtcaaccat ttcaagtcga agggctctga
     1441 ttgcgccagc atcggggacc ccgacctcgg cgacgggcag ggcttctgca acctgacccg
     1501 ggtggctcaa tcggaggcgc tcctcgattt cgtggacgag ttgagcgagg actcgccggg
     1561 tggcgtactg gtcatcggtg acctcaacag ctatcgcaac gaggatccaa tcgacaccct
     1621 ggaaggtggc gggctcaccg atctggtgga gtcctttgtc ggggaggacg cctactccta
     1681 cgtcttcgat ggccaactcg gctatctgga cacggccctg gcaaacgaca cgatgtcgga
     1741 caacgtgcag ggtgtcacca tcttccacat caacgccgac gagcccgaca tcctggatta
     1801 cgacatgtcg ttcaagcagg acgcccaaga cgcgctcttc gagcccctgc cttatcgagt
     1861 gtcagaccac gatccggtga tcgtcggtct gacgatcccg agaccgggac gcggcccaga
     1921 cagttgaaaa ccgtgggacg gtcgggattc ccggtcgtcc cacgactcac tcgagcaggg
     1981 cggctccggc agccaatgcc tccatcttgg cgtgggcaag ggatcgtttc agcgggacca
     2041 tgccgcagtt ggtggacggg tagatcctct ccagtggaac gtgcccggcg accgactcga
     2101 gcagggaggc gacatcctgc ggcgactcga cctcgtcggt cgcgacatcg atgaccccag
     2161 cctggacgct cttgtcaccg agcagatcga ggacttcgag tggcacatgg gagccggcca
     2221 tctctaagga gacctgatcg atcgatgagt cggccagcac cgggaaagtg acctcgtact
     2281 ggcgccactc gggaccgaga ccgcgtttcc agtcgatgtt ggccttgact ccatagccgt
     2341 aacagatgtg gacggccgtg atcgcatcga ctccttcgat cgccttctcc aacgcctcga
     2401 tgccccaccc accgacctcg tcgaggaaga cgttgaaagc gggctcgtcg aactggatga
     2461 tgtcggcccc cgcttctgcc atctcccggg cttcggcgtt gagcgcttcg gcgaagctca
     2521 tggcgagcgt ctctctgttg ccgtaatggt cgtcctgaca ggtgtccgcc atcgtcatcg
     2581 ggccgggcag ctgggtcttg atcctgagat cggtgagcga gcgcaggaaa cgggtctcgt
     2641 cgacgaacac accgccgcgt cgagacacct ccccgaccac ccgtggcacc gaggcgtcgt
     2701 atcgcctccg tatctgaacg gttgcccggt tgacggcatc gacaccctcg agcccttcga
     2761 tgaacgtcgt gacgaaatgc tggcgcgtct gctcgccatc ggtgacgatc tggagaccgg
     2821 cgcgctcctg ttcgaggaca gctacccgta ctgcatcgcg tttggcctcg gcgaggtcct
     2881 caccttcctg aacccagccc gcccacagct gatcgcgcga ggccaaccat gatggcttgg
     2941 ggaggctgcc ggcgattgtg gtggaaaggg tcaacgtagg caacggtaac cggccggcgt
     3001 tctaggagtc ggtgtcgccc tctcggtcca gctccggatc cgccgcctgc cgacgcccct
     3061 cgtcgtaacg ggacagattg agacggatgt cgatggcccc ctcccccgag accacactgg
     3121 gatcgtcctc tgtgaccgcg aagttggtgc cagggacccg ggtctgcagg cctggacctt
     3181 cctgggatgg catgaccggt gcgacatgac cgttgccgtt cgacttggcc ggccggcggg
     3241 tggccgatct gccccggagg ggaccgaaca ccttctcgag aaaggcctcc gcctccctac
     3301 gcgccgactc cgtcatcgcc acaacgtggc tggagcgata tgagctctcc ggtggcggct
     3361 ccgcctgatc atcgccttcg agcccaccga ctgtcgacac cagtgacgcc ggcaccgtca
     3421 cacgtgccgt tgtgccggga accgacggga ccaggcgcac atcgataccg tggcgcgcgg
     3481 cgagacgagc caccatggca atccccagcg acgagttgac ctccggccct gcccctcctg
     3541 aggaggccag gacttcgttg ag
//
LOCUS       DASUTA010000154         2078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1861894, whole genome shotgun sequence.
ACCESSION   DASUTA010000154 DASUTA010000000
VERSION     DASUTA010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1861894"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1329)
                     /locus_tag="VFZ80_03100"
     CDS             complement(145..1329)
                     /locus_tag="VFZ80_03100"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6286447.1"
                     /translation="MSEHGPLEGVLVVEFGNLIAAPYAGMLLADLGARVVKVEPLSGD
                     LGRQFGPYQRGESAFFMAVNRGKESVALDTKDWVSKRVLDNLVRKADVVLHNLRHGAM
                     ERLGLGEERCRELNQKVIYAVVSAFGPDGPYARRPGIDVIFQGESGMISITGDPKDGP
                     RKTATTIGDYVAGTNMALSIAAALAEQPRRGRRIDVSLRDGLMAVQSGWNAINFTTHT
                     QPERTGTASPFLSPNQVFEAADKPFTLAIVSDRHFELLCDAIERPDLAEEYPSNEDRM
                     ANRDSLIRKLTRLFKTEPAGYWIELLGQAALPVGRVLTISEAFDDPQARHHGMLVESE
                     HPVAGHIRTTGSPLRLDGRQARAESLPPRLGEHTRFVLKEMGVDAASIDKMIEEGRAV
                     SP"
     gene            complement(1322..>2078)
                     /locus_tag="VFZ80_03105"
     CDS             complement(1322..>2078)
                     /locus_tag="VFZ80_03105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX6286448.1"
                     /translation="NPEMQRLYPPITLGMTAEEVAAKYDVSREDQDEFALSSHHKALA
                     AIDAGRYDAEIAPIEAPADPRRRTTVTITSDEGPRPDTSLEVLAKLPPVFKEGGTVTA
                     GNSSPMNDGAAALLLATGEGLERHGLDPMARIVATGAAGVHPDIMGIGPVPASEKALG
                     RAGISADELDVIELNEAFASQSLASMRLLDLDPAKVNVNGGAIAIGHPLGASGARILT
                     TLVHELDRRRGRYGLATMCIGVGQGISTVIERV"
BASE COUNT          347 a          658 c          683 g          390 t
ORIGIN      
        1 gtcgacccag gagatcgtcg tggtgcacta catagacgaa ggactcctcc cgttgtgccg
       61 cagagcgact catctgaaag gcgcgctcga gctctgcgcg gacctcatcc cagtcatcgg
      121 caacctgctg cgccctgaag atgttcatgg gctcaccgcc ctgccctcct cgatcatctt
      181 gtcgatgctg gcagcgtcaa cgcccatctc cttgagcaca aagcgggtgt gctcccccag
      241 tcgcggtggg agagactctg ctcttgcctg tcggccatcg agtctcagag gggatccagt
      301 ggtgcggatg tggccggcaa ctgggtgctc cgactccacg agcatgccgt gatggcgagc
      361 ctgggggtca tcgaaggcct cgctgatggt gagaaccctt cccacgggca gagcggcttg
      421 cccgaggagc tcgatccaat acccggcggg ctccgttttg aacaggcgcg tcagctttct
      481 gatgagggag tcgcggttgg ccatgcggtc ctcgttgctc gggtattcct cggcgagatc
      541 agggcgctca atggcatcac agaggagctc gaagtggcgg tcggacacga tcgccaatgt
      601 gaacggcttg tctgctgcct cgaacacctg gttgggactc aggaaggggc tggcggtccc
      661 ggtccgctcg ggctgagtgt gagtggtgaa gttgatcgcg ttccaacccg actgaaccgc
      721 catcaggccg tcacgaagag agacatcgat cctgcgacct cgccggggct gctcggccag
      781 tgccgcggct atcgacaatg ccatgttcgt gcccgccacg taatccccga tcgtggtggc
      841 ggtcttacgc gggccgtcct tggggtcacc ggtgatcgag atcatgcccg actcgccctg
      901 gaagatgacg tcgatgccgg gccgacgtgc gtatggcccg tccggtccga aggccgagac
      961 gactgcgtag atcaccttct ggttcagctc gcgacaccgc tcctccccga gcccgagacg
     1021 ctccatggcc ccgtggcgaa gattgtggag gactacgtcg gccttgcgga cgagattgtc
     1081 gagaactcgt ttcgacaccc agtccttggt gtcgagggca accgactcct tgccacgatt
     1141 cactgccatg aagaatgccg actcgcctcg ttggtagggg ccgaactgtc gtcccaggtc
     1201 tcccgagagc ggctccacct tcacgactcg ggcgccgagg tcggcaagga gcattcctgc
     1261 gtagggagcg gcgatgaggt tgccgaactc gacgacgagg acgccttcga gcggtccgtg
     1321 ttcagacacg ctcgatgacg gtagagatcc cctgaccgac accaatgcac atcgtggcga
     1381 gaccgtagcg cccccggcgt cgatcgagct cgtggacgag tgtggtgagg atcctggcac
     1441 ccgatgcccc caggggatgg ccgatcgcaa tcgccccgcc gttgacattg accttcgcag
     1501 gatcgagatc gagtagacgc atgctcgcca ggctctgcga ggcaaaggcc tcgttcaact
     1561 cgatcacgtc gagctcgtcg gcggagatcc ccgcccgccc tagcgccttc tcggacgcgg
     1621 gcaccggacc gatccccatg atgtcggggt ggacccctgc cgcgccggtc gccacgatgc
     1681 gagccatggg gtcgagaccg tgacgttcga gcccttctcc cgtggcgagg agcagcgccg
     1741 cagccccgtc gttcatcggc gacgagttac cggccgtcac cgtcccgccc tccttgaaga
     1801 cgggaggaag cttcgcaagc acctcgaggg aggtatcggg gcgcggtcct tcgtccgaag
     1861 tgatggtcac ggtggtgcga cgacgcgggt cggctggtgc ctcgatgggg gcgatctcgg
     1921 cgtcgtaccg gccggcatcg atggcggcga gtgccttgtg atggctggac agggcgaatt
     1981 cgtcctgatc ctcccggctc acgtcgtatt tggcggccac ctcctcggcg gtcatgccca
     2041 aggtgatagg tggatacagc cgttgcatct cggggttg
//
LOCUS       DASUTA010000155         2789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_954116, whole genome shotgun sequence.
ACCESSION   DASUTA010000155 DASUTA010000000
VERSION     DASUTA010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954116"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="VFZ80_03110"
     CDS             complement(<1..133)
                     /locus_tag="VFZ80_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013615340.1"
                     /note="responsible for the addition of glutamate residues
                     to the C-terminus of ribosomal protein S6; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HEX6286449.1"
                     /translation="MKIAILSRGSSLYSTARLKEAALDRGHDVAIVDYLRCYMDITAR
                     "
     gene            complement(130..756)
                     /locus_tag="VFZ80_03115"
     CDS             complement(130..756)
                     /locus_tag="VFZ80_03115"
                     /inference="COORDINATES: protein motif:HMM:NF017434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HEX6286450.1"
                     /translation="MSIVLFPPCPVLTDMLISFLLWVMKSDMSSIRFDHTCVSNSTTW
                     PRSAHLPNLCVVAERKEKRKTIGWREWVSLPDLGVRELKAKVDTGADNSSLHAFDLER
                     FEEDGRQMVRFEIHPRQRKRRPSIPCVAEVVGERMVKNPGGRAERRPIIRTTLVVAGE
                     EFEALINLTTRDEMTFRMLLGRRTVRKNFIVDPGRSYLGDRPSRTVTE"
     gene            749..1162
                     /locus_tag="VFZ80_03120"
     CDS             749..1162
                     /locus_tag="VFZ80_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003981274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX6286451.1"
                     /translation="MLMRFDPFSDYDRLSRQVWGNTRSNFMPADAYRRDDRFYLHIDL
                     PGVDPDSVDVEVEKNTLTISAERRWDAGSEDQVLLNERQTGTFSRQFFLGESLDTDAI
                     EAGYDHGVLTVVIPIAETAKARKITVNSTPEAIEA"
     gene            1249..1569
                     /locus_tag="VFZ80_03125"
     CDS             1249..1569
                     /locus_tag="VFZ80_03125"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6286452.1"
                     /translation="MSQQVLSYKMALASLASDERYAEVFSHGELSAGIYAPHESDDQE
                     AHDQDEFYVVLNGSGFFTVDGEREPFGPGDLLFAPGGTPHQFEDFTPDFAVWAIFCGK
                     TETG"
     gene            1593..1739
                     /locus_tag="VFZ80_03130"
     CDS             1593..1739
                     /locus_tag="VFZ80_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286453.1"
                     /translation="MARHDKPMPAWLMGLIIAAVVFALALVVFNLLGFGDDPVVEGMA
                     WISG"
     gene            1724..2626
                     /locus_tag="VFZ80_03135"
     CDS             1724..2626
                     /locus_tag="VFZ80_03135"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014445097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX6286454.1"
                     /translation="MDFGVTAGARSNMPAAEVDIDEDLVRSLIRDQRPHLVGEELTPL
                     AFCWDNVSYRIGESRVARFPRREMAVQLIVNEATWLPELAPALPLAIPAPEYLGVPAQ
                     GYPWPWVISPFLRGETAATAHLDPLTGARQLGEFLKALHRPAPADAPDNRFRGGPLST
                     RAEGVEDRLGMITDAADRSRLADLWEESLHAPPHDGAPVWLHGDLHPLNLLVEDGTLT
                     AVIDFGDITAGDPATDLVVGWNLLGDGLEELWRAYGAEDEPLRLRARGWAIALGLAFA
                     DTSADHPVIREIGEKTLRAVLDNT"
BASE COUNT          515 a          882 c          870 g          522 t
ORIGIN      
        1 gccgggctgt gatgtccatg tagcagcgga ggtagtcgac gatcgcgaca tcgtggccgc
       61 ggtcgagcgc ggcctccttg agacgggcgg tcgagtacaa ggaggatccc cgggacagaa
      121 tggcgatctt cattcggtga ccgtcctgct cggtcggtcc ccgagatagc ttcggcccgg
      181 gtcgacgatg aagttctttc tcactgtcct cctgccgagg agcatgcgaa atgtcatctc
      241 gtccctcgtg gtcagattta tcaaagcctc gaactcctcg ccggcgacga ccagggtggt
      301 ccggatgatc ggcctccgct cggcgcgtcc tcctggattc ttcaccatac gctcgccgac
      361 gacctccgcc acgcacggaa tcgacggcct tctcttccgt tgacgaggat ggatctcgaa
      421 ccgaaccatc tggcggccat cctcctcgaa gcgttccaag tcgaaggcat ggagagagga
      481 gttgtcggca ccggtgtcca ctttggcctt gagctctctg acaccgagat cggggaggga
      541 tacccactcc cgccaaccga tggtctttcg cttctccttg cgttcggcca cgacacagag
      601 attagggaga tgcgccgagc ggggccacgt ggtcgagttg cttacacagg tatgatcaaa
      661 cctgatactt gacatatcag atttcatcac ccataataag aaggaaatca gcatatctgt
      721 taggacagga caaggaggaa agagcacgat gctcatgcgt ttcgacccat tctcggatta
      781 cgaccggctc tcccggcaag tctggggcaa cacccggagc aacttcatgc ctgcggatgc
      841 ctaccgacgg gacgaccggt tctatctcca catcgacctt cccggagtcg atcccgactc
      901 agtcgacgtc gaggtcgaga agaacacact gaccatctcc gccgagcgga gatgggatgc
      961 gggctctgaa gaccaggtcc tcctcaacga gcgtcaaacg ggaacgttct cgagacagtt
     1021 cttcctcggg gagagtctgg acacagacgc catcgaggcc gggtacgacc acggcgtgct
     1081 caccgtcgtg atccccattg ccgagaccgc caaggcccgc aagataaccg tcaactccac
     1141 gccggaggcc atcgaggcct gaacgcgggt ccgtacacag agacgccccg gccgcatcgc
     1201 gaaggcggcc ggggcgttgt cgcgtccgat cccggtacca tcggctcgat gagccagcaa
     1261 gtgttgtcat acaagatggc gctggcatca ctagccagcg acgagagata cgcagaagtc
     1321 ttcagccacg gtgagctcag cgcgggcatc tacgccccgc atgagagcga cgatcaggag
     1381 gcacacgacc aggacgagtt ctacgtggtc ctcaacgggt cggggttctt cacggtggac
     1441 ggagagcggg agccgttcgg gccgggagat ctcctgtttg cgcctggagg gacgccgcat
     1501 cagttcgagg acttcactcc tgacttcgcc gtgtgggcga tcttctgcgg gaagaccgag
     1561 accggctgat cgggccctag agtccgcgcc cgatggcgcg tcacgacaag ccgatgccgg
     1621 cgtggttgat gggactgatc attgccgctg ttgtcttcgc tctggctctc gtcgtcttca
     1681 acctcctcgg gtttggtgac gatccagtgg tggagggtat ggcatggatt tcggggtgac
     1741 cgcaggggcc cgcagcaaca tgcccgcggc ggaggtcgac atcgatgagg acctcgtgcg
     1801 ttcactcata cgtgaccagc gtccccatct cgtcggcgag gagttgacac cactggcgtt
     1861 ctgttgggac aacgtctcct atcggatcgg cgagagcaga gtcgcccgct tcccacggcg
     1921 ggagatggcc gtccagctca tcgtgaacga ggcgacatgg ctgccggagc ttgccccggc
     1981 cctcccgttg gccatccccg ctccggagta tctcggcgta ccggctcagg gctatccgtg
     2041 gccatgggtc atctccccgt ttctacgtgg tgagaccgcg gcaacggccc atctcgaccc
     2101 actgaccgga gcaagacagc tcggtgagtt cctgaaggct ctccaccgcc ccgcgcctgc
     2161 cgatgcgccg gacaaccggt tccggggagg accgctgtcg acccgcgccg agggggtcga
     2221 agatcggctc gggatgatca ccgatgcggc agaccgcagc cggctggcgg atctctggga
     2281 ggagtcattg cacgcgcctc ctcacgatgg cgctccggtt tggctgcatg gcgacctcca
     2341 tcctctcaat ctcctcgtcg aagatgggac gctcaccgcg gtgatcgact tcggcgacat
     2401 cacggccggc gatccggcca cggacctcgt agtgggatgg aatctccttg gcgacggcct
     2461 cgaggagctg tggcgggcct acggcgccga ggacgagcct ctgcgtctcc gagcccgggg
     2521 ctgggcaatt gccctgggat tggccttcgc cgatacctcg gccgaccatc cagtcatcag
     2581 agagatcggg gagaagaccc tcagagcggt cctcgacaac acctgactcg ccgaggtcgc
     2641 cagcgcctca cgactcaggg atcacattcc ccttgcagtc gatgccgtcc gctgggcgat
     2701 agttggcctc gatggtgtcg accatcaccc ggccatctga gaaggttcgc tgccggtcga
     2761 tctccgagtg ccggcactgg tttcggaac
//
LOCUS       DASUTA010000156         5695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_436292, whole genome shotgun sequence.
ACCESSION   DASUTA010000156 DASUTA010000000
VERSION     DASUTA010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436292"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2902)
                     /gene="rpoC"
                     /locus_tag="VFZ80_03140"
     CDS             complement(<1..2902)
                     /gene="rpoC"
                     /locus_tag="VFZ80_03140"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019851643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX6286455.1"
                     /translation="MPAQIFDELRIGLASNDQVRAWSYGEVKKPETINYRTLRPEKEG
                     LFDERIFGPQRDWECYCGKYKRIRYKGIICERCGVEVTRSKVRRERMGHIELAAPVTH
                     IWFFKGVPSRLGYLLDMSPKELEKVIYFASYIVTSVNDESRQKDLPELEEAMGDEIEV
                     VKSEFKEWHEVRLEALEDELERMEEGGAKTQEINARRKEVEKEIKEREERTNREIELI
                     EEAFSTFRTMGPKQLIESEQLWREIYFRYADYFEGGMGAEAVKTLLTSLDLDAEMDKL
                     EENLNGSSQQRRQRATQRMKVVKPFWEGQNRPEDMILETVPVIPPDLRPMVQLDGGRF
                     ATSDLNDLYRRVINRNNRLKRLLDLGAPEIIVNNEKRMLQEAVDALFDNGRRGRAVTG
                     PGNRALKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKIMALEL
                     FKPFVMKRLVDLDLAQNIKAAKRMVERQRPQVWDVLSDVIQEHPVLLNRAPTLHRLGI
                     QAFEPVLVEGKAIQIHPLVCEAFNADFDGDQMAVHLPLSAEAQAEARILMLSSNNVLS
                     PASGRPIVTPSQDMVIGIFYLSELVEDAKGAGRTFADIDEAFMAYEIGELSLHAPIKL
                     RLGELGGDPDVHNDLSQTLKKLVNEEPVDPKALTETTLGRALLNTAFPEDFPYINSAV
                     IKSDIKLLIETIIERYPRSAVAATLDGIKELGFQFATRAGLTIALDDVRTPADKAEIL
                     AEYEERAEKVQNLFAKGVVTDDERRQELIEIWTEATEKVTDAMKAGLKTEQFNSLDMM
                     VGSGARGNMMQVRQIAGMRGLVANPKGDIIPRPIISNFREGLSVLEYFISTHGARKGL
                     ADTALRTADSGYLTRRLVDVSQEMIVTDEDCETDRGIVIKVVDERGNKIRNLRSRIYG
                     RMLADDLKEETEKGGHALVATEDDEKLRAGTIIGTKELHAIEEADVESVRVRS"
     gene            complement(2966..>5695)
                     /locus_tag="VFZ80_03145"
     CDS             complement(2966..>5695)
                     /locus_tag="VFZ80_03145"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX6286456.1"
                     /translation="NTIERDTNADKEEALLDLYRKLRPGEPTTVESARGLIQTLFRNP
                     KRYDLTRVGRYKIAQKFGEDSPKDYRAEGLQMLRDEDIVDAIRYLVALHAGQATMQTH
                     QGNEISISTDDIDHFGNRRIRTVGELVQNQVRVGLTRLERVVRERMTTQDPDAITPQS
                     LINIRPVTAAIKEFFGTSQLSQFMDQPNPLAGLTHRRRLNALGPGGLSRERAGFEVRD
                     VHPSHYGRMCPIETPEGPNIGLIGSLACYARVNDFGFIETPYRKVENGKVSDKVDYLT
                     ATEEERYVIAQANAPLNEDGSFVNKRVLVRRAGGEVETTDANNVDYMDVSPKQIVSVS
                     TSLIPFLEHDDANRALMGSNMQRQAVPLLTAESPLVGTGVEARAAQDSGDMIISPVAG
                     EVVEVTGDEIVLKEKGANKKHTFALDKFKRSNQGTCMNQKPIVSVGDQVKEGDVLADG
                     PSTQAGELALGRNLLVAFMPWQGHNFEDAIIISERLVKDDVLTSIHIEEHEIDARDTK
                     LGAEEITRDIPNVPEEVLANLDDEGIIRVGAEVGPGDYLVGKVTPKGETELTPEERLL
                     RAIFGEKAREVRDTSLKVPHGETGKVIAVKVFKREDGFDLSPGVNELVRIYVATQRKI
                     SEGDKLAGRHGNKGVISKVLPEEEMPFLPDGTPVDIILSPLGVPSRMNLGQVLETHLG
                     WAAAQGWEEFGGDSPAAPGAKPWTKTATPVFDGAREDEIMKVIANALPNRDGDRLVGE
                     DGKAVLYDGRTGEPFDTAVTVGYIYMLKLVHLVDEKIHARSTGPYSMITQQPLGGKAQ
                     FGGQRFGEMEVWALEAYGAAYALQELLTIKSDDVQGRVKVYEAIVKGDNIPEPGIPES
                     FRVLVKEMQSLGLNVEMLSAGEEVELRELEEDTYRSVETLGIDLSRPERPDSE"
BASE COUNT          931 a         1770 c         1798 g         1196 t
ORIGIN      
        1 gtgaccgcac ccggaccgac tcgacgtcgg cctcttcgat ggcatgcagc tccttggtcc
       61 cgatgatggt tccggcgcgg agcttctcgt cgtcctcggt agcgaccagg gcatggccgc
      121 ccttctcggt ctcctctttg agatcatcag caagcatgcg gccatagatc ctcgacctca
      181 ggtttctgat cttgttgccg cgttcgtcga cgaccttgat cacgatgccg cgatctgtct
      241 cacagtcctc gtcggtgacg atcatctctt gtgagacgtc gaccagacga cgggtcaaat
      301 agccggagtc ggcggtgcgg agcgccgtgt cggccagacc cttgcgggcc ccgtgggtcg
      361 agatgaagta ctcgagaacg gacagccctt cccggaagtt ggagatgatc ggccgcggga
      421 tgatgtcacc cttcgggttg gccaccagac ctcgcatccc ggcgatctga cgcacctgca
      481 tcatgttccc tcgggcaccc gacccgacca tcatgtcgag cgagttgaac tgctcggtct
      541 tgagaccggc cttcatcgca tcggtcacct tctcagtggc ctcggtccaa atctcgatca
      601 actcctgacg acgctcgtca tcggtgacga cgccctttgc gaagaggttc tgaaccttct
      661 cggccctctc ctcgtactcg gcgaggatct cggccttgtc ggcaggtgtc ctcacgtcgt
      721 cgagggcaat ggtcagaccg gcccgggtcg cgaactggaa gcccagctcc ttgatcccgt
      781 cgagagtcgc ggcgacagcc gagcgcgggt agcgctcgat gatcgtctcg atgagcagct
      841 tgatgtcgct cttgatcacc gccgagttga tgtatgggaa gtcctcgggg aaagccgtgt
      901 tcagcaaggc acggccgaga gtggtctcgg tcagggcctt gggatcgacc ggctcctcgt
      961 tgaccagctt cttcagcgtc tggctcagat cgttgtggac gtcggggtcg cctccgagct
     1021 ctcccagacg cagcttgatc ggcgcgtgaa gcgatagctc gccgatctca taggccatga
     1081 aggcctcgtc gatgtcggcg aacgttcgtc ccgctccctt ggcgtcctcg acgagctccg
     1141 agagatagaa gatgccgatg accatgtcct gggacggcgt gacgatcggt cggcccgatg
     1201 ccggcgagag cacgttgttc gaggacagca tgaggatacg cgcctcggcc tgggcttcag
     1261 ctgacagcgg caggtgcaca gccatctggt caccgtcgaa gtccgcgttg aacgcctcac
     1321 agaccaacgg atgaatctga atggccttgc cctcgacgag cacaggctcg aaggcctgga
     1381 tgccgagacg gtgcaaggtc ggggcacggt tgagaagcac ggggtgctcc tggatgacgt
     1441 cgctgagcac atcccagacc tgaggccgct gacgctccac catgcgcttc gccgccttga
     1501 tgttctgcgc caggtcgagg tcgacgagac gcttcatgac gaacggcttg aaaagctcga
     1561 gggccatgat cttgggaaga ccgcactggt gcagcttgag ctgagggccc accacgatca
     1621 ccgatcgacc ggaatagtcg actcgcttgc cgagcaggtt ttgacggaac cggccctgct
     1681 tgcccttgag catgtccgac agcgacttca gtgcgcggtt gcccggtccc gtcacggcac
     1741 ggccgcgtcg gccgttgtcg aacagcgcgt cgaccgcctc ctggagcatg cgcttctcgt
     1801 tgttgacgat gatctcggga gcgccgagat cgagcagcct cttcaggcgg ttgttcctgt
     1861 tgatcacccg tcggtagaga tcgttgaggt cggaggtggc gaaccggcca ccgtcgagct
     1921 gcaccatggg gcgcaggtcg ggcgggatga ccggcacggt ctccaggatc atgtcctcgg
     1981 gacggttctg gccctcccag aagggcttga ccaccttcat cctctgagta gcccgttgac
     2041 gacgttgctg ggaggagccg ttgaggttct cctcgagctt gtccatctcg gcgtcgaggt
     2101 ctagggaggt gagcaaggtc ttgaccgcct cggcacccat gccgccctcg aagtagtcgg
     2161 cgtagcggaa gtagatctcg cgccacagct gctcggactc gatgagctgc ttggggccca
     2221 tggtgcggaa cgtgctgaac gcctcttcga tcagctcgat ctcacgattg gtgcgctctt
     2281 cgcgctcttt gatctccttc tcgacttcct tgcgccgggc gttgatctcc tgggtcttgg
     2341 cgccgccttc ctccatgcgc tccagctcgt cctcgagcgc ctcgagacga acctcgtgcc
     2401 actccttgaa ctcgctcttg accacctcga tctcgtcgcc catggcctcc tcgagctcgg
     2461 gcaggtcctt ctgacgggac tcgtcgttga ccgaggtcac gatgtagctg gcgaagtaga
     2521 tgaccttctc cagctccttc ggcgacatgt cgagcaagta gccgagacgg ctgggaacgc
     2581 ccttgaagaa ccagatgtgg gtcaccggag cggcgagctc gatgtggccc atccgctctc
     2641 gtcgcacctt ggaccgggtc acctcgactc cgcaacgctc gcagatgatc cccttgtaac
     2701 ggatcctctt gtacttaccg caatagcact cccagtcccg ctgggggccg aagatgcgct
     2761 cgtcgaagag accctccttc tcgggacgaa gggttcggta gttgatcgtc tccggcttct
     2821 tcacctctcc gtaggaccag gcgcgcacct ggtcgttgga ggcgaggccg attctcagtt
     2881 catcgaatat ctgggcaggc ataggctcgc ttcgctcgca gtgtcaggtt ggtatttgct
     2941 atttcgtatt cggactaaca agcgcctatt ccgagtcggg gcgctcggga cggctgaggt
     3001 cgatgccgag ggtctcgacc gatcggtagg tgtcctcctc gagctcgcgg agctcgactt
     3061 cctcaccggc ggagagcatc tcgacattga gaccgaggct ctgcatctct ttcaccagga
     3121 cccggaacga ctccggaatg cccggctcgg ggatgttgtc gcctttgacg atggcctcgt
     3181 agacctttac ccggccctgg acgtcatcgg atttgatggt cagtagctcc tggagggcgt
     3241 aggcggcgcc ataagcctcc aaagcccaga cctccatctc gccgaacctc tgaccgccga
     3301 actgggcctt accgcccaac ggctgctggg tgatcatgct gtacggaccg gtcgaacggg
     3361 catggatctt ctcgtccacg aggtggacca gcttcagcat gtagatgtac ccgaccgtca
     3421 ccgcggtgtc gaagggctca ccggtgcggc cgtcgtagag gaccgccttg ccgtcctcgc
     3481 cgaccagacg gtcgccgtcc ctgttgggga gggcgttggc gatcaccttc atgatctcgt
     3541 cctcgcgagc accgtcgaag acgggggttg cggtcttggt ccacggcttg gctccgggag
     3601 cggccggcga gtcaccaccg aactcctccc agccctgcgc cgcggcccaa ccgagatgag
     3661 tctcgaggac ctgtccgagg ttcattcggc tcggcacacc cagcggggac aggatgatgt
     3721 ccacgggggt gccgtcgggg aggaatggca tctcctcttc ggggagcacc ttggagatga
     3781 ctcccttgtt gccgtgacgc cctgccagct tgtcgccttc tgagatcttg cgttgtgtgg
     3841 cgacgtagat gcggacgagc tcgttgacac cggggctgag gtcgaatccg tcctcgcgct
     3901 tgaacacctt gacggctatg accttgccgg tctcgccgtg gggcaccttc agagacgtgt
     3961 cccggacctc acgcgccttc tcaccgaaga tggcccgaag gagacgctcc tccggggtca
     4021 actcggtctc tcccttcggg gttactttcc cgaccaggta gtcacccggg ccgacttctg
     4081 cgccgacgcg gatgatgccc tcgtcgtcga ggttggccag gacctcctct gggacattgg
     4141 gaatgtcccg ggtgatctcc tcggcgccga gcttcgtgtc ccgtgcgtcg atctcgtgct
     4201 cttcgatgtg gatcgaggtg agcacgtcgt ctttgacgag acgctcggag atgatgatgg
     4261 cgtcctcgaa gttgtgcccc tgccatggca tgaaggccac gagaaggttg cggccaaggg
     4321 ccagctcgcc ggcctgggtc gagggtccgt cggcgagcac atccccttcc ttgacctgat
     4381 cccccacaga cacgatcggc ttctggttca tgcacgtgcc ctggttggac cgcttgaact
     4441 tgtcgagagc aaaggtgtgt ttcttgttgg cgcccttctc cttgaggacg atctcgtcac
     4501 ctgtcacctc gacgacctca ccggcgaccg gcgagatgat catgtctcct gagtcctggg
     4561 cagcccgtgc ttcgacaccg gtcccgacca gcggtgactc agcggtgaga aggggcacgg
     4621 cctggcgttg catgttggag cccatgaggg cgcggttagc gtcgtcgtgc tccaggaacg
     4681 ggatcagcga agtggagacc gagacgatct gcttcgggga cacgtccatg tagtcgacgt
     4741 tgttggcgtc ggtggtctcg acctctccgc cggcgcggcg gaccaagact cgcttgttga
     4801 cgaaggagcc gtcctcgttg agaggagcat tggcctgtgc gatgacatag cgctcctcct
     4861 cggtggccgt gaggtagtcg accttgtcgg agaccttccc gttctcgacc ttgcgatacg
     4921 gcgtctcgat gaaaccaaag tcgttgaccc tggcgtaaca ggccagtgat ccgatgagac
     4981 cgatgttcgg gccctccggt gtctcgatag ggcacatgcg gccgtagtgg gaggggtgga
     5041 cgtcgcgtac ctcgaacccc gcccgctccc ggctcaggcc acctgggccg agcgcattga
     5101 gccggcgacg atgggtcaat ccggccagcg ggttgggctg atccatgaac tggctgagct
     5161 ggctcgtccc gaagaactcc ttgatcgcag cagtgaccgg tcggatgttg atcaggctct
     5221 ggggagtgat cgcgtccggg tcctgggtcg tcatccgctc acgaaccact cgctcgagac
     5281 gggtcagacc gacgcgtacc tggttctgaa ccagctcgcc gacggtgcgg attcgccggt
     5341 ttccgaagtg atcgatgtcg tcggtggaga tcgagatctc gttgccttga tgagtctgca
     5401 tcgtggcctg gccggcatga agcgcgacca gatatctgat ggcgtcgacg atgtcctcgt
     5461 cgcgaagcat ctgaagcccc tcggcgcggt agtccttggg ggagtcctcg ccgaatttct
     5521 gggcgatctt gtaccgaccc accctggtga ggtcatagcg cttgggatta cggaagaggg
     5581 tctgaatgag cccccgagcc gattcgaccg tcgtcggctc cccgggacgg agcttgcggt
     5641 agaggtcgag aagagcctct tccttgtccg cgttcgtgtc gcgctcgatg gtgtt
//
LOCUS       DASUTA010000157         2389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_696575, whole genome shotgun sequence.
ACCESSION   DASUTA010000157 DASUTA010000000
VERSION     DASUTA010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696575"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(389..811)
                     /locus_tag="VFZ80_03150"
     CDS             complement(389..811)
                     /locus_tag="VFZ80_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286457.1"
                     /translation="MEDRERIRIWSEPPPFGRVVFDGGWLGTLAGGVVGLLPSLAGAF
                     FLIPIGAVVGLAHGMFFALGAWAGGWIAVRLGIAKRKVLALFIGLVVTGLVYPFVITA
                     ILDQVDLLLPWVVSFLAVAIGAFRGCDYGRLREPSVTD"
     gene            complement(922..1278)
                     /locus_tag="VFZ80_03155"
     CDS             complement(922..1278)
                     /locus_tag="VFZ80_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033990 - ectoine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine synthase"
                     /protein_id="HEX6286458.1"
                     /translation="MLIRHLDQISNTERDVFGQGWRSRRLVVADDGLRYSFHVTTLDE
                     GARLEFEYEDHRETVYCIQGEGQIQDLATGRTERIKPGTIYSAGIGEPHVITADTSMS
                     LVCVFDPPLAGTEEAT"
     gene            complement(1331..2125)
                     /locus_tag="VFZ80_03160"
     CDS             complement(1331..2125)
                     /locus_tag="VFZ80_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6286459.1"
                     /translation="MISRQVAYQNKIFFRTPIAAFFTLVFPVLLLVIFAALFGSEEIE
                     ELGVTVAQYYAPALAVFAAASATYTNIGVNTAYQRDLGILKRVRGTPLPPWIFFVGKV
                     ISATLIAAIAVAVMLALGVIAYGITIYPRTLPAAIVTFFVGTASFAGLGLLVAAVAPS
                     GGAATAIANATLLPLAFFSGLFIPFSDDTPAWIRTVGDIFPLKHFNEAFQKAFLPGTT
                     GAQFDWPALAIMAAWGIAALLLAVRLFKWEKGQPSRGRVREAATST"
     gene            complement(2160..>2389)
                     /locus_tag="VFZ80_03165"
     CDS             complement(2160..>2389)
                     /locus_tag="VFZ80_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286460.1"
                     /translation="RQLSATRISFTHSGDQPLPIGAAVENGMVVIDSEEPIQDLHRLT
                     TWAIDNAIAIGDIEVSRPSLEDVYLSLTDDG"
BASE COUNT          460 a          723 c          769 g          437 t
ORIGIN      
        1 cttcgtgccg gccgaccagg ctcgggcccc agtggatgcc gcgctcggcg cgggagggag
       61 actcgccgac gattcccagg ccatcgcctg gtggtcgctc gcgtcccctg tcaaccacgg
      121 cgtcgacatc gcgtcctgga ctgacaccta cgactgactc gcgcgcacgt acctcttggg
      181 tggtccttga gattctcgtc taacgagatc tcccgaagac tcgaagggtt cgcccacatt
      241 cactccagag gcggctcagt ccccggcgcg gcgccggagg ccgagattcg tggttgaggc
      301 agcgacgagg aatccaatga atcggacacc gaaccgccaa gtctttctga ccgaagcact
      361 gatggctatc tcagttctta ccggtgtgct aatcggtgac tgagggttct ctgagccggc
      421 cgtagtcgca tcctctgaat gcgccgatgg cgacggcgag gaagctcacc acccagggga
      481 gtagaaggtc cacctgatcg aggatggcgg tgatcacaaa cggatagacg agcccagtta
      541 ccaccaagcc gatgaatagc gccaggacct tccgcttggc aatgccgagg cgtacggcga
      601 tccagcctcc tgcccaggcg cccagcgcaa agaacatgcc gtgggcgaga ccgacgacgg
      661 cgccgatggg gatgaggaag aatgcgccag cgagagacgg aagcagtcca acgaccccgc
      721 cggccagtgt gcccagccag cctccatcga agacgacccg gccgaagggc ggtggctctg
      781 accagatccg aattcgctct ctatcctcca caaccctgtc cttcgccaca agacgtgccc
      841 tctgctctca caagtctggt ccatggatgg caggcgttgc gggcacttcg gcgcatcggg
      901 gacgatcgga aggttcgtcg gtcaggtcgc ttcttcggta ccggccaggg gcggatcgaa
      961 cacacagacc aggctcatgc tggtgtccgc agtgatgacg tgcggctcgc cgattccggc
     1021 ggagtagatg gtgcccggtt tgatccgctc ggttcgtccg gtggcgaggt cttggatctg
     1081 gccttcgccc tgaatgcagt agacggtctc gcggtggtct tcgtattcga attcgaggcg
     1141 tgctccctcg tcaagcgtgg tgacgtggaa ggagtagcgg agcccgtcgt cggccacaac
     1201 cagtcgtcgg cttctccacc cctggccgaa aacgtcgcgt tcggtgtttg agatctggtc
     1261 gaggtggcgg atcagcatcg ccggactgta gtgccaggcc ttcctccgat cagggccacg
     1321 aacaacgagt tcaggtgctc gtagccgcct cccggactct ccctctggat ggctggccct
     1381 tttcccattt gaacaatctg accgcgagca ggagagcggc tatcccccat gccgccatga
     1441 tcgccagggc gggccagtcg aactgggcgc cggtggtccc cgggaggaag gccttttgga
     1501 aggcttcgtt gaagtgcttc aacgggaaga tgtcgccaac ggtgcgtatc caggccgggg
     1561 tgtcgtcgct gaagggtatg aacaatccgg agaagaaggc caaagggagc agcgttgcat
     1621 tcgcaatggc cgtggcggct cccccgctgg gagcgactgc cgcgaccagg agccccaagc
     1681 ccgcgaaaga ggccgttccg acgaagaagg tcacgatcgc cgccggcagg gtgcgtgggt
     1741 agatggtgat cccgtaggca atcacaccca aagcgagcat taccgctact gcgatcgccg
     1801 cgatgagggt ggcagagatc actttgccga cgaagaagat ccagggtggc aagggcgttc
     1861 cccggacccg tttcagaatc cccaggtctc gttggtatgc ggtgttgacc ccgatgttgg
     1921 tgtaggtggc cgaagcggca gcgaatacag cgagggccgg ggcgtagtac tgggcgacgg
     1981 tcacgccgag ctcttcgatc tcctcgcttc cgaagagggc ggcgaatatc accagcagca
     2041 acaccgggaa gaccagagtg aagaacgccg cgatgggcgt ccggaagaag atcttgttct
     2101 ggtaggcgac ctgtctcgag atcacggtcc aagtcgagtt cctcgtgctc tcgaccccct
     2161 taaccgtcat cggtcagact cagatagacg tcctccaggg aaggtctgct gacctcgata
     2221 tccccgatcg caatcgcatt gtcgatcgcc caggtggtga gacgatggag atcttggatc
     2281 ggttcctccg aatcgatgac gaccatcccg ttctccactg cggcaccgat tggcagaggc
     2341 tggtcgccgc tgtgcgtgaa tgagatccgg gtcgccgaga gctgacggt
//
LOCUS       DASUTA010000158         3069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_48853, whole genome shotgun sequence.
ACCESSION   DASUTA010000158 DASUTA010000000
VERSION     DASUTA010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48853"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VFZ80_03170"
     CDS             <1..667
                     /locus_tag="VFZ80_03170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEX6286461.1"
                     /translation="LAKAGIAQAYYQAFGADHNHLERIKDELSAMTKGWDKTEIEALV
                     TETVDEVITPLVYAEALAIIDEHRGKGRRVVVVSASPEEIVRPLCSYLGIGDVIATRA
                     AVDEEGRYTGEIELYAYGSGKADAIRAMAEEEGIDLESSYAYSDSITDLPMLEIVGHP
                     VVVNPDSELAAIARGRGWEVRRFESTVTIRDRLPTIAASGAVVTGIAAILAYWALKGR
                     RSG"
     gene            complement(645..977)
                     /locus_tag="VFZ80_03175"
     CDS             complement(645..977)
                     /locus_tag="VFZ80_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286462.1"
                     /translation="MISIESLAEHLAGDWVAFDKYGNVLDYIVPNDIGPATLAVSPVT
                     DALKEVDEADRVVGSVDKNAVWSVDAIVLNSIVLRRLPNDAYEAEDLIQAVRDAGFSW
                     QISPTSVP"
     gene            1024..1674
                     /locus_tag="VFZ80_03180"
     CDS             1024..1674
                     /locus_tag="VFZ80_03180"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HEX6286463.1"
                     /translation="MTSRAMIIVGGGSSTRFGADKLLADIDGLPLVAYTIEAMAAHAD
                     RCVVVCRPETVEEIEKLRPDVIVTAGGSTRTLSEMAGLTALGGEADLIGIHDAARPVV
                     KGSIIDRLFTLADRYGGAIPALSPEQLILDRKTHRPLPGLRRAQTPQVFRGPDLMAAY
                     VKAARVGYEGQDTSEIMERFGNVTITVLPGDRENLKVTYPTDIEKVTESIKGRSRT"
     gene            complement(1652..2140)
                     /locus_tag="VFZ80_03185"
     CDS             complement(1652..2140)
                     /locus_tag="VFZ80_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6286464.1"
                     /translation="MSDEVRWAVPDDADALARVHITSWQTAYRGIFSDEFLDGLDLAA
                     RQRWWRNRLEEGARVPVVGDPAVGFCFVGDADDEGWGEVYAIYVHPHHWGEGLGRRLL
                     SAGSDLLRDTGHTRALLWVLRDNQRARRFYESLGWQVSRPIRVEEIGGTQVTEVRYET
                     DL"
     gene            complement(2133..2660)
                     /locus_tag="VFZ80_03190"
     CDS             complement(2133..2660)
                     /locus_tag="VFZ80_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286465.1"
                     /translation="MELIALIASVVIGGLFGTASASVEAIGEDVMIVEIEVEVPPGAA
                     NVVAHLSFESEDLVLPLTDRGDGVFGIRTELPPRNYFVVFEVIGEEGGSSEAVSLTEL
                     GAELTGGPAATATTAPDDEGLDDDSRQMLWLAVALGAASLSALAFWVLGGRDQDDERA
                     EPVEEDEPASADHDE"
     gene            complement(2651..>3069)
                     /locus_tag="VFZ80_03195"
     CDS             complement(2651..>3069)
                     /locus_tag="VFZ80_03195"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX6286466.1"
                     /translation="DGLAEPLADLIDDLDAHDRLIVGSFSDTRITEIQEVTERRVPVS
                     CGPTLARLWVLASRVGRGAGGDIAALQLPARVRGVRVVDEKLVAAAHASDLHVHVWTV
                     NQTSEMHRFLDMGVDGLITDRPDRLRDVMIERELWN"
BASE COUNT          609 a          981 c          970 g          509 t
ORIGIN      
        1 cttggccaag gccggtattg cccaggccta ctaccaggcc ttcggggccg accacaacca
       61 cctggagcgg ataaaggacg agctctccgc aatgaccaag ggttgggaca agaccgagat
      121 cgaggccctg gtcacagaga cggtcgatga agtgatcaca ccactggtct acgcagaagc
      181 actggccatc atcgacgagc atcgtggaaa ggggcggcgc gtcgtcgtcg tatcggcaag
      241 ccccgaggag atcgtcagac ctctctgcag ctatctggga atcggggatg tgattgctac
      301 cagggcagcc gtggacgagg agggccgata taccggagag atcgagctgt acgcctacgg
      361 gagtggaaag gcggatgcga tccgagccat ggcggaggaa gaaggcatcg acctcgagtc
      421 cagctacgcc tacagcgact cgatcacgga tctccccatg ctcgagatcg tcggccatcc
      481 agtcgtggtg aacccggact ccgagctggc cgcaatcgcc cgtggccgtg ggtgggaggt
      541 ccgcaggttc gagtcgactg tgacgattcg cgatcggctc ccgaccatcg ctgcctcggg
      601 cgcggtggtc accggaatcg cagcgatcct cgcctattgg gccctcaagg gacggaggtc
      661 gggctgatct gccaggagaa gccggcgtct cgcactgcct gtatcaggtc ctcggcctcg
      721 taggcgtcat tggggaggcg acgaagcacg atggagttga ggacaattgc atctaccgac
      781 caaacggcgt tcttgtcgac ggatcccacc accctgtcgg cctcgtcgac ctccttcaag
      841 gcatccgtca ccggagacac cgccagggtg gcggggccga tgtcgttggg gacgatgtag
      901 tcgagaacgt tcccgtactt gtcgaatgcc acccagtctc cggcgaggtg ctcggcaagt
      961 gattcgatgg agatcacgtg aaggcctcta cgaggtgcac gaggcgaaag tagcctgccg
     1021 cacgtgacgt cgcgggcgat gatcatcgtt ggtgggggct cgagcaccag gtttggtgcc
     1081 gacaaactgc tggcggacat cgatggcctg ccgttggttg cctacacgat cgaagccatg
     1141 gccgcgcacg ccgatcgttg cgtagtcgtg tgcaggccgg agacggtcga ggagatagag
     1201 aagctgagac ccgacgtcat cgtcaccgcg ggcggatcga cacggaccct gagcgagatg
     1261 gccggcctca ctgcactcgg tggcgaggcc gacctcatag gcatccatga tgcagcacgg
     1321 ccggtggtga aaggatccat catcgatcga ctgttcacac tcgcagatcg ctatggggga
     1381 gcaatccccg ccctgtcgcc cgagcaactg atactcgatc gcaaaacgca tcgacccctg
     1441 cccgggctgc gtcgggccca gacgccgcag gttttcaggg ggcccgacct gatggctgcc
     1501 tatgtgaagg cggctcgggt gggctacgaa ggacaggaca cgtcggagat catggagcgc
     1561 ttcggcaacg tgacgatcac tgtcctgccc ggtgaccgcg agaacttgaa ggtgacctac
     1621 cccaccgaca tcgaaaaggt gacagagagc atcaaaggtc ggtctcgtac ctgacctcgg
     1681 tcacctgagt cccgccgatc tcctcgacac gaatcggcct gctcacctgc cagcccaggc
     1741 tctcatagaa ccggcgggcg cgctggttgt cgcgcagaac ccagaggagg gctctcgtat
     1801 ggcccgtgtc gcgaagaaga tcggagccgg cagagaggag ccggcgaccg agcccttcgc
     1861 cccagtgatg cgggtggaca tatatcgcgt agacctcgcc ccatccctcg tcgtcggcat
     1921 caccaacgaa acagaagcca acggcggggt cacccacgac cggcacacgg gccccctcct
     1981 cgagacgatt ccgccaccat ctctgtcgtg ccgcgagatc gaggccatcg aggaactcgt
     2041 cggagaagat cccccggtag gcggtctgcc atgaggtgat gtgcacacgg gcgagggcat
     2101 cggcatcgtc ggggacggcc cagcggacct cgtcactcat cgtggtcggc agaggccggc
     2161 tcgtcctcct caactggctc ggcccgctca tcgtcttggt ccctgccccc caagacccag
     2221 aaggcgaggg cggagagcga cgccgcaccg agagccacag cgagccagag catctgacga
     2281 gaatcatcgt ctagaccttc atcatcgggg gccgtcgtcg cggtggcggc gggcccgccc
     2341 gtcaattctg cacctagctc ggtcagcgac acggcctcac tgctcccgcc ctcttccccg
     2401 atcacctcga agacgacgaa gtagttgcga gggggcagct ctgtcctgat gccgaacacg
     2461 ccatctcccc gatcggtgag cggaagcacc aggtcctcgc tctcgaagct gagatgagcg
     2521 accacgttgg cggcgcctgg ggggacttcg acctcgatct cgacgatcat gacatcctca
     2581 ccgatcgcct cgaccgatgc actcgctgtc ccgaacagcc ccccgatgac aaccgatgcg
     2641 atcaaggcga tcaattccac aactcccgtt cgatcatcac gtctctgagc cggtccgggc
     2701 gatcggtgat gagaccgtcc acacccatgt cgaggaagcg gtgcatctcc gacgtctgat
     2761 tcacggtcca cacatgaacg tgtaggtcgg aggcgtgagc ggctgccacc agcttctcgt
     2821 ccacgacccg gaccccgcga actctcgccg ggagctgaag cgcagcgatg tcgccaccag
     2881 cgccccttcc cacccgggac gcaagcaccc agagacgggc cagagtcggg ccgcacgaca
     2941 ctggcacacg cctctctgtc acctcctgga tctcggtgat tcgggtgtct gagaaggagc
     3001 cgacgatgag gcgatcatgt gcgtcgagat cgtcaatgag gtctgcgagt ggttcggcca
     3061 gaccatcac
//
LOCUS       DASUTA010000159         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1474191, whole genome shotgun sequence.
ACCESSION   DASUTA010000159 DASUTA010000000
VERSION     DASUTA010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474191"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VFZ80_03200"
     CDS             <1..406
                     /locus_tag="VFZ80_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286467.1"
                     /translation="GVAVWGASPTGLALAIAARESDPRLVAVAHPDLAIDTFDRTTRF
                     PDPVGRVGVREAQLDGRPVAEAHSPNEFAACLAVGVKRRVTVVDHGHFLSGVALAAGV
                     AVANGAVTAVWENALPYLEAVSEMGLVMAESD"
     gene            complement(478..1059)
                     /locus_tag="VFZ80_03205"
     CDS             complement(478..1059)
                     /locus_tag="VFZ80_03205"
                     /inference="COORDINATES: protein motif:HMM:NF023511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HEX6286468.1"
                     /translation="MATVRRVRRIIRKIDPWTTLKVSALVWAVLALAFVLGAVIFWSV
                     LDRAGIPDRLVEFMVEITLIEEGSDPFANTEQFLRFLIFSSVVGWVVMTGTTVAIAVI
                     YNLVSDVVGGLEIVVLEETLNPVASPATQQQVLHPPADYRAVNGGLAPPPDQADLPTE
                     EVEPVVETPVAEDHDQDDEAAAAEEPERVERIG"
     gene            complement(1245..>2524)
                     /locus_tag="VFZ80_03210"
     CDS             complement(1245..>2524)
                     /locus_tag="VFZ80_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEX6286469.1"
                     /translation="AVDNIDEVIKIIRGSADTPEARENLIATFDLSEIQANAILDMPL
                     RRLTALEVDKLRTELAELRDTIAELESILADPKRRWSIIKEELGSIRETFAEDRRSRL
                     VPDEGDLSLEDLIADDELIITVSNTGYLKSVPASTYRTQGRGGRGVKAAEVRDDDVVR
                     HMVHTTAHAYLLFFTNKGLVHRIKAHGIPRQSRTAKGVMAQSVLPIGPDESIEAVIDT
                     RDYETGRYLVMVTRNGQAKKTEFKEYDSRNQTLVAIKLTDGDELVTVRTSNGSNDILI
                     FTEGGMGIRFAEKDLRSMGRGTQGVRGIKLRDGDRVVGAVSNIDGDEVLLLTSGGYGK
                     RTEMEEFRRQTRGGLGVKAFKLTKVRGNLVDARAVRAEDEVFLISSSGVGVRQAVGRI
                     SRQKRDSTGVKVISLSEGNHLTAVAKISEELDE"
BASE COUNT          425 a          787 c          807 g          505 t
ORIGIN      
        1 gggcgtcgca gtctggggtg catccccgac cggtctggcc ctggccatcg ccgcccgtga
       61 gtcggatcca cgtctggtgg ccgtcgccca ccccgacctg gcgatcgaca ccttcgacag
      121 aacgaccaga ttcccggatc cggtgggccg cgtcggtgtg cgcgaggcgc agttggatgg
      181 gagaccggtg gccgaggccc actctcccaa tgagtttgcg gcctgcctcg ctgttggcgt
      241 caaacgccgg gtcaccgtgg tcgaccacgg gcatttcctc tccggggtcg ccctggccgc
      301 cggagtggct gtcgccaacg gagcggtgac cgccgtctgg gagaacgctc tcccttatct
      361 ggaggcggtg agcgaaatgg gtctggtcat ggccgagagc gattaggtca gttgtggcgt
      421 tgctatagca tcgtctagcg gtttccgagg catgacgcca tggcaacgcc gtacgtgtca
      481 accgatgcgt tcgacccgct cgggctcctc ggccgccgca gcctcgtcgt cctggtcgtg
      541 gtcctcggcg acaggcgttt cgaccacggg ctccacttct tcggtgggga ggtcggcttg
      601 gtcggggggc ggggccaaac ctccgttgac tgctcgatag tcggccgggg ggtggaggac
      661 ctgttgctgg gtagcgggtg aggccaccgg gttgagcgtc tcttccaaca ccactatctc
      721 gagcccaccg accacatcgg aaaccaggtt gtagatcacg gcgatcgcca cagtggtccc
      781 ggtcatcaca acccatccca ccaccgaact gaagatgagg aagcgcagaa actgctcggt
      841 attggcgaag gggtcactcc cctcctcgat cagggtgatc tcaaccatga actcgacgag
      901 acggtcggga atgccggcgc gatcgagcac cgaccagaag atcacggctc ccaggacgaa
      961 tgcgagagcg aggacggccc aaaccagggc ggacaccttc aacgtggtcc atgggtcgat
     1021 cttgcggatt attcgccgga ctcggcggac agttgccatt ggggctcctg attgtggctc
     1081 ggttgtggga gtctcctcag gaaactccac actctccagc gtgttggata ggcgaagtct
     1141 aagggactgc ccggttacac caacgtcacc tgcgccggca ccggttcctg aagatgagct
     1201 gatttgggtc acatatggcc ccaggaaccc cgggttcgga gttgctactc gtccaactcc
     1261 tcggagatct tggccacggc ggtgagatgg ttgccctcgc tgagggagat gaccttgacc
     1321 cccgtggagt cgcgcttctg gcggctgatc ctgcccaccg cctgacgaac accgaccccg
     1381 gaagaggaga tgaggaacac ctcgtcctct gctctgacgg cgcgggcgtc gacgagattc
     1441 ccccgaacct tggtcagctt gaacgccttc acaccgagtc caccacgtgt ctgtctgcga
     1501 aactcttcca tctcggtgcg cttcccgtag ccaccggagg tgagcagcaa gacttcgtcg
     1561 ccatcgatgt tggataccgc accgacgact cgatcgccgt cgcgcagctt gatcccgcga
     1621 acaccctgag tgccgcgacc catgctcctc aggtccttct cggcgaaccg gattcccatg
     1681 ccgccctcgg tgaagatgag gatgtcgttg gagccattcg aggtgcgcac ggtgaccaac
     1741 tcgtcgccgt cagtgagctt gatcgccacc aacgtctgat tgcgcgagtc gtactccttg
     1801 aactcggtct tcttcgcctg tccgttccgg gtcaccatga ccaggtagcg cccggtctca
     1861 tagtcccggg tatctatgac ggcctcgatg ctctcatcgg gcccgatggg aagcaccgac
     1921 tgtgccatca ctcccttcgc ggttctcgac tgtctgggaa tgccgtgggc cttgatacgg
     1981 tgcaccaggc ccttgttggt gaagaagaga aggtaggcat gcgccgtggt gtgcaccatg
     2041 tgccgaacga catcgtcgtc tctcacctcg gcggccttga ctccccgacc accgcggccc
     2101 tgtgtgcggt aggtggaggc cgggaccgac ttcagatagc cggtattgga gacagtgatg
     2161 atgagctcgt cgtcggcgat gaggtcctcg agactcaggt cgccttcatc gggcacgaga
     2221 cggctccgtc ggtcctcggc gaaggtctcc cgtatcgagc ccagctcctc tttgatgatc
     2281 gaccagcggc gcttggggtc ggcgaggatc gactcgagct cggcgatggt gtcgcgtagc
     2341 tcggccagct cggtgcgcag cttgtcgact tcgagggcgg tgagccggcg caggggcatg
     2401 tcgaggatgg cattggcctg gatctcactc agatcgaacg tggcgatgag gttctcacgc
     2461 gcctcgggag tgtctgccga tcctctgatg atcttgatga cctcgtcgat gttgtcgacg
     2521 gcga
//
LOCUS       DASUTA010000160         2391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_49201, whole genome shotgun sequence.
ACCESSION   DASUTA010000160 DASUTA010000000
VERSION     DASUTA010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49201"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFZ80_03215"
     CDS             complement(<1..198)
                     /locus_tag="VFZ80_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX6286470.1"
                     /translation="MTETISLIAGSGWASGINLYLVALMLGIAGRSGWADIPDVLQRT
                     DVMIVAGVLFAVEFFADKVPFL"
     gene            complement(195..824)
                     /locus_tag="VFZ80_03220"
     CDS             complement(195..824)
                     /locus_tag="VFZ80_03220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6286471.1"
                     /translation="MGDVPALSIEIVNLVADMAPALEEIELLCFPMANPEDLLSADDI
                     VAYAEVFPEGYFVALLDGVPVGQGAGIYLDFDFDRPQHTIAGITGEHQCGNHDPNGDW
                     YYGTDISVHPDHRGLGIGSMLYEARKDLVRRAGKRGIIAGASLPGYFEHKEYMSAAEY
                     VEDVVAGRLTDPTLSFQMSHGFEVRGMLENYLEDEADDGWAALIVWEAS"
     gene            complement(803..1834)
                     /locus_tag="VFZ80_03225"
     CDS             complement(803..1834)
                     /locus_tag="VFZ80_03225"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEX6286472.1"
                     /translation="MFGGEMRVLEIFGDSADLGRAHGSACSEMIRKYTNERIRLTTQE
                     DWSGGQAAHDLILSCAEETLPHHEQFSENLYGEMLAMASAAGITPPEAVAVGGFTDLV
                     DVVRTRIGAAGEEHNCTGIINPKTGLFAQTWDMHASAGEFVIMLKIDPLMGPDAFVQT
                     TAGCLGQIGMNEAGIAIGINNLTSSGKPGVTWPFVVRKVLEQTDLDDAVKVVLDADLA
                     GGHNYFLMGPDGEGATIEAMPRNKRVTRTNGAPLVHANNCLYPETKADEARRLQDWVA
                     NSDERLRLGEQYADDLDAFFADPMISRRVDDLDEVGTCGAVIIDPVKRKMRAVWGIPG
                     DHPWETFRL"
     gene            complement(1848..>2391)
                     /locus_tag="VFZ80_03230"
     CDS             complement(1848..>2391)
                     /locus_tag="VFZ80_03230"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX6286473.1"
                     /translation="PRYVVGVIGAAAAALSLATDGHTVSQGNVALNASLDVVHVLAAG
                     IWVGGIVGLLVLTVRRRRRLEQGSMATTIVRFSSVATLAIVSVAAAGFGMSLLIIDSI
                     ADYVTTDWGRLLLLKVALVAIAVGLGAYNHFVAVPALEVDPTDSLVLSKARRTTSAEA
                     AILLVVTLLTVFLTGASINQ"
BASE COUNT          449 a          776 c          721 g          445 t
ORIGIN      
        1 gagaaaaggc accttgtcgg cgaagaactc gacggcgaac aggacaccgg ccacgatcat
       61 cacgtcggtg cgctgaagga cgtcggggat gtcggcccag ccggaacgtc cggcgatccc
      121 gagcatcaac gccaccagat agaggttgat gcccgatgcc cagcccgaac cggcgatgag
      181 gctgatggtc tcggtcacga cgcttcccag acgattaggg ccgcccatcc gtcgtcggcc
      241 tcgtcctcga ggtagttctc gagcatgccc ctcacctcga acccatggct catctggaag
      301 ctgagtgtgg gatcggtcaa gcggccggcg accacatcct cgacgtactc ggccgccgac
      361 atgtactcct tgtgctcgaa gtagcccggt aacgatgcgc cggcgatgat cccccttttc
      421 cccgccctcc tcaccagatc tttacgcgct tcgtagagca tcgacccaat gcccaacccc
      481 cgatggtcag gatggacact gatgtcggtc ccgtagtacc agtccccgtt cgggtcgtgg
      541 ttgccacatt gatgctcacc ggtgatcccg gcgatcgtgt gctgtggccg atcgaaatcg
      601 aagtcgaggt agatccctgc accctgcccg acgggcacac cgtcgagtaa cgcgacgaag
      661 tacccctcgg ggaacacttc ggcgtaagcc acgatgtcgt ctgccgacag cagatcctcc
      721 gggttggcca tgggaaagca gagaagctcg atctcttcga gcgctggggc catgtcagcc
      781 accaggttga cgatctcgat gctcagagcc ggaacgtctc ccaagggtgg tcgccgggaa
      841 tgccccacac cgccctcatc ttgcgtttga ccggatcgat gatcacagcg ccacaggtgc
      901 ccacctcatc gagatcgtcg acgcgtcggg agatcatcgg atctgcgaag aaggcgtcga
      961 ggtcgtcggc gtactgctcc ccgaggcgaa gacgttcgtc gctgttcgcc acccaatcct
     1021 gcagccgccg tgcctcgtcc gccttggtct ccggatacag gcagttgttg gcgtgaacca
     1081 gcggcgctcc gttggtccgg gtgactcgct tgttgcgtgg catggcctcg atcgtggcgc
     1141 cctcgccgtc agggcccatc aggaagtagt tgtgccctcc tgccaggtcg gcgtcgagga
     1201 cgactttgac cgcgtcgtcg aggtcagtct gctccaggac cttcctcacc acgaacggcc
     1261 aggtgacgcc gggcttcccc gacgacgtca ggttgttgat accgatggcg atacctgcct
     1321 cgttcatgcc gatctgaccg agacatcccg ccgtggtctg cacgaaggca tccgggccca
     1381 tgagcgggtc gatcttcaac atgatcacga attcgcccgc cgacgcatgc atgtcccacg
     1441 tctgtgcgaa gagcccggtc ttcgggttga tgatgcccgt gcaattgtgc tcttcgcccg
     1501 ctgctccgat tcgagtgcga accacgtcga cgaggtcggt gaaccctcca accgcaaccg
     1561 cctctggtgg tgtgatgccg gccgccgaag ccatggcgag catctcgccg tagagattct
     1621 ccgagaactg ctcgtggtgg ggaagagtct cctcagcgca gctcaggatg aggtcgtggg
     1681 ccgcctggcc accgctccaa tcctcttggg tggtcaatcg gatccgctcg ttggtgtact
     1741 tgcggatcat ctccgagcat gccgaaccgt gagctcgacc caggtctgcc gagtctccaa
     1801 agatctccaa gacgcgcatc tcaccgccga acatgtttga agattaccta ttggttgatc
     1861 gatgctcccg tcaggaacac cgtgagcagg gtcacgacaa ggaggatcgc ggcctcggca
     1921 cttgtggttc tcctcgcctt ggagaggacg agggagtcgg tcgggtcgac ctccaatgca
     1981 ggcacggcca cgaagtggtt gtaggcaccg agtcccactg cgatggccac caatgcgacc
     2041 ttgagaagga gcaatcgtcc ccagtccgtc gtcacgtagt cggcgatcga gtcgatgatc
     2101 agaagggaca tgccgaaacc ggccgctgcc accgagacga tggcaagggt cgccacggat
     2161 gagaatctga cgatcgttgt cgccatcgag ccttgctcga gtcggcgtcg acgccgaacg
     2221 gtcaatacga gcaatccgac gatcccgccc acccagatgc ccgctgcgag cacgtgaacc
     2281 acgtcgagcg atgcgttgag cgccacattg ccctgcgaga cggtgtgccc atcggtggcg
     2341 agcgagagcg ccgccgccgc cgccccgatc acgccaacga catatcgcgg a
//
LOCUS       DASUTA010000161         3316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_955841, whole genome shotgun sequence.
ACCESSION   DASUTA010000161 DASUTA010000000
VERSION     DASUTA010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955841"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFZ80_03235"
     CDS             complement(<1..416)
                     /locus_tag="VFZ80_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286474.1"
                     /translation="MISDHPASPPPVSRPWIAAAGFVWLVMSAIGSPHHPPLEHGVFN
                     GPLVLLTDSYQGHYGPWAIGAVSTGRVLVDFEENPPLSRGDTAIVFGEIGGGPGVAAG
                     RGFGGVLRVQDIAEVTRSRFVPHRAGALLRRRVDDAL"
     gene            complement(413..979)
                     /locus_tag="VFZ80_03240"
     CDS             complement(413..979)
                     /locus_tag="VFZ80_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEX6286475.1"
                     /translation="MGENRNTIVFAAILMVFTVAVGAGIGLMRRPPLPAPSSDAMVTS
                     PAPGTGAVAIEVHVAGWVNNPGVVTVGEDAIVADAIAAAGGMRSGADADGVNLAAALT
                     AGQQVVVPGPDDSSGVGGAPDAGGLVSLNRATASELESLPGVGPVLAGRIVSHRESHG
                     PFEEVDDLLQVPGIGEAKLAALRDLVQP"
     gene            complement(1074..1364)
                     /locus_tag="VFZ80_03245"
     CDS             complement(1074..1364)
                     /locus_tag="VFZ80_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286476.1"
                     /translation="MATLHLPTSPGRDFLYDQEDLEMEESKTLSRVRDARKAIATAQK
                     AMENAQTGLAGVESVAEKADQVRRHPLATTAVILGSGLAATVTFLGIKGSSD"
     gene            1390..2202
                     /locus_tag="VFZ80_03250"
     CDS             1390..2202
                     /locus_tag="VFZ80_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEX6286477.1"
                     /translation="MLDVSHYRIPPDAKVALSDHDPDAKTGFEAGKIEGKKALPPLND
                     RLAELQHRLWAESQQSLLVVIQAIDTGGKDSTIRKVFTGVNPNGVRIASFGVPTELEL
                     AHDYLWRVHKETPEDGYIKVFNRSHYEDVLVVRVKQLAAKATWSRRYRHIREFERMLA
                     DEGTTIVKFFLNISREEQRERLQARLDDPEKHWKFNKTDLDDRALWDDYQEAFEDALS
                     ETSTDYAPWYVVPANRKWYRNLVVSTVLIETLERMNPQFPEPEEGLDEIVIQ"
     gene            complement(2230..2631)
                     /locus_tag="VFZ80_03255"
     CDS             complement(2230..2631)
                     /locus_tag="VFZ80_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286478.1"
                     /translation="MNAGRILRKAGLDGDELRAAIAPVDPDTVNVWPASRSMMRLWRR
                     GIKGVTIRSWVFVDPEILRGDADRLGRLVIHELVHVRQFNESGYVPFSLRYVYEYLGG
                     VLQGKGLRQAYLDISAEQEARAVTEALLHPV"
     gene            complement(2628..>3316)
                     /locus_tag="VFZ80_03260"
     CDS             complement(2628..>3316)
                     /locus_tag="VFZ80_03260"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX6286479.1"
                     /translation="GNVVDPVALFASHGADSLRLYHLFMGPPTDDAAWNENGVDGTRR
                     FLERVWKIGTDDHHFSGREPDDSDRELVGLAHRTVRKVTEDIDRFHFNTAVPALMVLS
                     NEFGEYLSAEPVETTYHEVLRLLLLLMAPMTPHIAHELWEANGYGDMLSTESWPAWDE
                     DLAREETVTMVIQVNGKVRDRLEVSADITSAQAEETALSSEKIQEWLDGQEVERVIAR
                     PPNLVNLVVG"
BASE COUNT          658 a         1082 c          982 g          594 t
ORIGIN      
        1 agtgcatcgt cgacccgtcg tcgcagcaga gctccggctc ggtgcgggac gaagcgactc
       61 cgcgtgacct ctgcgatgtc ctgaactcga aggacacctc caaacccgcg cccggccgca
      121 actcccggcc caccgccgat ctcaccgaag acgatggcgg tatcacctct cgaaagcggt
      181 ggattctcct cgaagtcgac caggactcgt ccggtggaca cggcaccgat ggcccacggg
      241 ccgtagtggc cctgatagga gtcagtgagt aagaccaacg ggccgttgaa gacaccgtgc
      301 tccaacggcg gatgatgtgg actgccgatc gccgacatca ccagccagac aaaccctgca
      361 gccgcgatcc agggccggga gaccggcggg ggtgaggcag gatgatccga gatcaaggct
      421 ggaccaggtc ccgcaaggct gcgagcttgg cctcaccgat ccccggaacc tggagaaggt
      481 catcgacctc ttcgaacggg ccatgcgact ctctgtggga gacaatcctc cccgcgagca
      541 ccggcccgac acctggcaag ctctcgagct cgctcgcggt ggcccgattc aacgacacca
      601 gccctccggc gtcaggcgcc ccacccaccc ctgaggagtc atccggtccg ggaacgacca
      661 cttgttggcc ggctgtgagg gcagcggcga gattcacacc atcggcgtcg gctccggatc
      721 gcatgccacc agctgctgcg atggcatcgg cgacgatggc gtcctccccg acagtcacca
      781 ctcccgggtt gttcacccag cccgccacat ggacctcgat tgccaccgct ccagtgcctg
      841 gcgcaggaga cgtgaccatc gcgtcacttg acggcgcggg cagaggtggg cgacgcatca
      901 agccgatgcc ggcgcctact gcaacggtga acaccatcaa gatcgctgcg aagacgatcg
      961 tgtttctgtt ctcccccaca tgcgtctact tacaggctgg ctctacccgt ttgaacctct
     1021 ctgacgtcgg aggcgccgtt ccaacgccat tgctcgacgg ccttgctgac taatcagtcg
     1081 ctcgagccct tgatcccaag gaaggtgacg gtggctgcaa gtccggatcc gagaatgacc
     1141 gcggttgtcg ccaacgggtg acgacgaacc tgatcggctt tctcggcgac cgattcgaca
     1201 ccggccagtc cggtctgggc gttctccatc gccttctggg cggtggcgat cgccttccgt
     1261 gcgtcacgga cgcgactcaa cgtcttgctc tcttccatct ccagatcctc ctggtcgtac
     1321 aggaagtctc tacccgggct ggtcggcagg tgaagcgttg ccaacgctcc gacggtaggg
     1381 tcgccgacca tgctcgacgt gtcccattat cggatcccgc ccgatgccaa ggtcgccctg
     1441 tccgatcacg accccgacgc caagaccggg ttcgaggcgg gcaagatcga aggcaagaag
     1501 gctttgcctc ccctgaacga tcgactcgcc gagctccagc acaggctctg ggccgagtca
     1561 cagcagagct tgttggtggt gatccaggcg atcgacaccg gaggcaaaga ctccaccatt
     1621 cgcaaggtct tcacaggtgt caacccaaat ggggttcgga ttgccagctt tggcgtgccc
     1681 accgaactcg agctcgcaca cgactatctg tggcgtgtgc acaaagagac ccccgaggac
     1741 gggtacatca aggtcttcaa tcgctcccat tacgaggacg tgctcgtggt cagagtcaaa
     1801 cagctcgctg caaaggccac ctggagccgc cgctaccgcc acatccggga gttcgagcga
     1861 atgctcgcgg acgaaggcac gacgatcgtg aagttcttcc tcaacatatc gagggaggag
     1921 cagagggaga ggcttcaggc ccggctggac gacccggaga agcactggaa attcaacaag
     1981 acagacctgg atgaccgggc actctgggac gactaccagg aggcgttcga ggatgctctc
     2041 agcgaaacat cgaccgacta cgcgccatgg tatgtggttc ccgcaaaccg caagtggtat
     2101 cgaaatctgg tggtctccac tgtcctcatc gagaccttgg agaggatgaa tccccagttt
     2161 ccggagcctg aggaggggct cgacgagatc gtcatccagt gaaggctccc tacccacaca
     2221 ccgtggcaat cacacagggt gaagaagcgc ttcggtcact gccctggcct cctgctccgc
     2281 cgagatgtcg agatacgcct gacgaagccc cttgccttgg aggactcctc ccagatactc
     2341 gtacacgtat cggagcgaga aggggacgta gccagactca ttgaactgtc ggacgtggac
     2401 caactcgtgt atcacgaggc gcccgagccg gtcagcgtcg cccctcagta tctccgggtc
     2461 gacaaagacc cagctgcgga tggtcacgcc cttgatgccc ctgcgccaaa gcctcatcat
     2521 cgaccgcgag gcgggccaca cattcaccgt gtccgggtcc acgggagcga tggcagcgcg
     2581 gagctcatca ccatccagcc ccgccttccg caggatgcgc ccggcgttca cccgaccacg
     2641 aggttgacca gattcggcgg acgagcgatg accctttcca cctcctggcc atccagccac
     2701 tcctggatct tctccgaaga cagagcggtc tcttctgcct gagcggatgt gatgtcagcc
     2761 gacacctcga gtcggtcgcg aaccttcccg ttcacttgaa tcaccatggt gaccgtctct
     2821 tcacgggcga gatcctcgtc ccatgccggc catgactcgg tggagagcat gtcgccgtat
     2881 ccattcgcct cccacagttc gtgtgcgatg tgtggggtca tcggggccat cagcagcaag
     2941 agcagtctca gcacctcgtg gtaagtcgtc tccaccggtt cggctgacaa gtattcgccg
     3001 aactcgttgg ataggaccat cagtgcaggg actgcagtat tgaaatggaa acggtcgatg
     3061 tcctcggtca ccttgcgcac cgttcgatgc gccagaccga ccagttctcg atccgagtcg
     3121 tcgggctccc tacccgagaa gtggtgatca tcggtgccga ttttccatac tcgctcgagg
     3181 aatcgtcgcg tcccgtcgac accgttctcg ttccaggcag cgtcgtcggt gggggggccc
     3241 atgaacaggt gatagagacg aagcgagtcg gcgccgtgag aggcgaatag ggcgaccggg
     3301 tcgacgacgt tccctt
//
LOCUS       DASUTA010000162         2433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1474719, whole genome shotgun sequence.
ACCESSION   DASUTA010000162 DASUTA010000000
VERSION     DASUTA010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474719"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VFZ80_03265"
     CDS             <1..625
                     /locus_tag="VFZ80_03265"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HEX6286480.1"
                     /translation="TIFPTPGTPTLVLDSGANPEVKADQLAQFAIMGSVAATALLGAD
                     PARVGLLSIGEEKGKGRELERAAYDLIEAGPVHFVGNVEGRDVATDRVDVIVTDGFTG
                     NIFLKATEGTASLLNTYILEAVSTLPPEQQAAVMPALAKVKERMDWENYGGAQLLGIK
                     GAALIAHGSSSHRAIYNALLMARDSADKDLPGKVTAALGESAQASLL"
     gene            622..1296
                     /gene="rnc"
                     /locus_tag="VFZ80_03270"
     CDS             622..1296
                     /gene="rnc"
                     /locus_tag="VFZ80_03270"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019435505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX6286481.1"
                     /translation="MSFEEGLGYTFAEPALLRLALTHRSVSSDDPARHDNERLEFLGD
                     AVLQLVVTELLYESYPQLAEGQMAKVRAAVVSRGTLSEIARALDLGSHIELSPSEAAT
                     GGREKDSILADGVEAVIGAIYLDGGLEPARGMVLHFWKERVAERAKRPGVKDYKTRLQ
                     ELVAREGKRPVYRVKGAGPDHDRRFVAVVSVNGTDYGSGEGRSKKQAEQAAAEIALDA
                     LSRDPK"
     gene            complement(1277..2170)
                     /locus_tag="VFZ80_03275"
     CDS             complement(1277..2170)
                     /locus_tag="VFZ80_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286482.1"
                     /translation="MLISPRPFGQVLADAVNSLGRIWRSMLFPALAVSIPVSIATAVV
                     FRVTGGSDFLELIFNSPENLAGLPSEIFWELARPFYVALAIATVLQILAGVFVALASH
                     GAVAADLRGEPLNGGDVSRLALKRYPVGVGSTVLIVLSVAVLIALGTLLWLMPAVEVG
                     TPNPGSVFVALLLFFALLGPGIWVAVSVSMTTSAIAIEDQGVLGSIRRSMRLVRGRWW
                     ATAGFLLLVGLLGGIAIQLIQLVALPLATVGDGGGALTIASALGVLTQGLLVAAIAAM
                     YTHWYIDLRARREGLTTSDLG"
BASE COUNT          499 a          762 c          802 g          370 t
ORIGIN      
        1 caccatcttc ccaacgcccg gcacgccgac actggtgctc gactccggtg ccaaccccga
       61 ggtgaaagcc gaccagctgg ctcagttcgc aatcatgggc tcggtggcag cgaccgccct
      121 gctcggagcc gacccggctc gcgtcggtct cctctcgatc ggggaggaga aggggaaggg
      181 gagagaactg gagcgagccg cgtacgacct gatcgaggcg ggccccgttc acttcgtggg
      241 gaatgtcgaa ggacgtgatg tcgccaccga cagagtcgat gtgatcgtca ccgacggatt
      301 caccgggaac atcttcctga aggccactga aggcacggcg tcactcctga acacctacat
      361 cctcgaggcc gtctcgacac ttccgccaga acagcaagcg gccgtgatgc ccgctctggc
      421 gaaggtcaag gagaggatgg attgggagaa ctatgggggc gctcagttgc tgggcatcaa
      481 gggcgccgct ctcatcgccc acgggagctc tagccatcgg gctatctaca acgccctgtt
      541 gatggcccgg gacagcgccg acaaggatct gcccgggaaa gtgaccgccg cgctggggga
      601 atcggcacaa gccagcctgc tatgagcttc gaagaagggc tgggttacac ctttgcagag
      661 cccgctcttc tcaggcttgc actcactcac cgctcagtca gctcggacga cccggcgcgc
      721 cacgacaacg agaggctcga gtttcttggt gatgctgtct tgcagctggt cgtgaccgag
      781 ctcctctatg agtcatatcc ccagttggcc gaaggtcaga tggcaaaggt cagggcggcg
      841 gtggtcagcc gcggaaccct cagtgagatc gcgcgagcac tcgacctggg ctcacatatc
      901 gagctctctc cgagcgaggc tgccacgggg ggccgggaga aagactccat tctcgccgac
      961 ggagtggaag ccgtgatcgg tgccatctac ctcgacgggg gtctcgagcc ggcacgaggc
     1021 atggtcttgc acttctggaa ggaacgtgtg gccgaacggg ccaagcgacc aggcgtgaag
     1081 gactacaaga cccggcttca agaattggtg gcccgagaag gcaagcgacc ggtctatcgg
     1141 gtcaaaggag ccgggccgga tcacgaccgt cgtttcgtgg ccgtggtctc ggtcaacggc
     1201 accgactacg gctccggtga gggccgttcg aagaagcagg ccgagcaggc cgcggccgag
     1261 atcgctctgg acgccctcag ccgagatccg aagtagtcag tccctcccgc cgggcgcgaa
     1321 ggtcgatgta ccagtgggtg tacatggctg cgatcgcggc taccagcaga ccctgggtga
     1381 gcacccccaa cgccgaggcg atggtcaatg ctccgccgcc gtctccgacg gttgccagag
     1441 ggagggcaac gagctgtatg agctgtatgg cgatccctcc caagagaccg acgagcagga
     1501 gaaagcccgc agtcgcccac caacgccctc tgacgagacg catgctgcgt ctgatcgagc
     1561 ccaacacgcc ctggtcctcg atggctatcg ccgatgtggt catggacacc gacacggcga
     1621 cccagatccc ggggccgagc agggcgaaga acaggagcag ggcgacgaac acgctgccgg
     1681 ggttcggggt tcccacctcg actgcgggca tgagccacag caaggtcccg agcgctatca
     1741 agacagccac tgagaggacg atcagcacgg tggagccaac accgacgggg tatcgcttca
     1801 gtgcaagacg ggacacatcg cctccgttga gaggctctcc ccgcaggtcg gcggcaaccg
     1861 caccatggct ggccagggcg acgaagaccc cggcgaggat ctgcaggacc gtggcgatcg
     1921 ccagagccac gtagaaggga cgggccaatt cccagaagat ttcgctgggc agccctgcga
     1981 gattctcggg gctgttgaag atcaactcga ggaagtcact gcccccggtg acccggaaca
     2041 ccaccgcagt cgcgatgctg acaggtatcg agacggccag cgccgggaag agcatcgacc
     2101 gccagatccg gcccaggctg ttgaccgcgt ctgccagcac ctgcccgaag gggcgggggg
     2161 atatgagcat gagcgcgggt acggtacctg cgtctcggtg gaatccggca ggttggactt
     2221 ctccacgaga actcccagaa tcgtcacgac acccatgccg gagctccccg aagtagagat
     2281 cacgagaagg caccttgagg cagcgttgct cgaccgcgtc ttcaccgacg tctccctgac
     2341 tcatgcgcga actgctcgtc acaacgcgtc cccgcaggaa gtggagacga ggctgcgggg
     2401 ccgccgggtg cgatcggtgg gacgacaagg caa
//
LOCUS       DASUTA010000163         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_50660, whole genome shotgun sequence.
ACCESSION   DASUTA010000163 DASUTA010000000
VERSION     DASUTA010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50660"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VFZ80_03280"
     CDS             <1..340
                     /locus_tag="VFZ80_03280"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6286483.1"
                     /translation="QGSAYAKLFNPASRYAIVGACAVVDVDGDGNCTACTVAVGGLTA
                     KATLLESVATGLVGNPITEESAESAAQAAVDEIGDNVMGDLYAGEDYRRDVLPVFVAR
                     ALTAAGSRAG"
     gene            402..665
                     /locus_tag="VFZ80_03285"
     CDS             402..665
                     /locus_tag="VFZ80_03285"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX6286484.1"
                     /translation="MTRPCFHFRRLETEADPLSDVCGQCLESGDTWVHLRSCLVCGQV
                     GCCDNSKNRHARKHWEQAGHALVRSLEPGETWKYCFPDHFLME"
     gene            734..1234
                     /locus_tag="VFZ80_03290"
     CDS             734..1234
                     /locus_tag="VFZ80_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6286485.1"
                     /translation="MKVSMTINGRAVTADVEPRTLLVHFIREQMRLTGTHVGCETGYC
                     GACTVHLDGKAVKSCTMFAVQAEGADIKTIEGVASGGALHPVQEGFWERHGLQCGFCT
                     PGMIMSAIALLDENDNPSEAEIRHGIEGNLCRCTGYHNIVRAVEYAAAKMRGEEPPPP
                     DWERTS"
     gene            1234..2091
                     /locus_tag="VFZ80_03295"
     CDS             1234..2091
                     /locus_tag="VFZ80_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6286486.1"
                     /translation="MYPRQFDYIAPTSIEEALAALGEDSKVMAGGMSLIPLMKLRLFS
                     PGLVVDIGRIPDLDGVAEFGDHIEIGALVRHATTATHPLIRNHAPALATAASLTGDVQ
                     VRNRGTTCGAFAHSDVAADQPAGALACGAVMIARSSEGTREIPAADFFIDSLTSALEP
                     DEILISARFPKVGPGEGSAYDKLGRRGGHSDYAVAGAAAWVRTSNGSITDARVAVTGV
                     GTKPQLTGAVAEALIGTDGSDSAIEAAAEKAADDITVLEDLYGSEEYKTHLAKVFAGR
                     AVKAALANV"
     gene            complement(2101..>2983)
                     /locus_tag="VFZ80_03300"
     CDS             complement(2101..>2983)
                     /locus_tag="VFZ80_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286487.1"
                     /translation="DDLASLPNVYIDAGEVRLNLIPFIGEALRRVADDVRAVLPDFDL
                     PDAISDRLSEGKDQLAAALQASLPEDFGQVTVMSESALAEVQTVAVQLDRLVWATLAL
                     TLVLLVLTLVASPNRRKTAIHLGLGVFVAIVIASVAIRRLQEAVVGQIVDPRGSVLAE
                     GLVRDALSGLRAIQLILAVAAVLVAVVAYLAGRPQWFVTLNRTIEEWTETGPDGSRLD
                     RWIAPHTGALLFGGVVIAALAFFLVGLDLLSVVVIGLLLGGYIWAITAASSRGSAGDR
                     VEGPGSETAQKRSNEPV"
BASE COUNT          584 a          955 c          951 g          493 t
ORIGIN      
        1 tcagggctcg gcttacgcca agctcttcaa cccggcttct cgctacgcca tagtcggcgc
       61 ttgcgcggtc gtcgacgtcg atggcgacgg caattgcacg gcatgcaccg tcgccgtcgg
      121 agggctcacc gccaaagcca cgctcttgga gtcagtggcc accgggctgg tgggcaatcc
      181 gatcacggaa gagagtgccg agtccgcagc gcaggcggcg gtggacgaga tcggtgacaa
      241 cgtcatggga gacctatatg ccggagagga ctaccgacgt gacgtgttgc ccgttttcgt
      301 tgcccgagca ctgacggcag ccggatcgcg cgccggttga gcgaataccc tgacgaaagg
      361 aggtccccac tcccgggggc ctccttttcg gcatcagcga catgacgaga ccctgctttc
      421 acttcagacg gctcgagacc gaggccgacc cactgtcgga cgtgtgtggc cagtgcctgg
      481 agtcaggaga cacctgggtc cacctgcggt cgtgtctcgt ctgcgggcag gtcggctgtt
      541 gcgacaactc gaagaaccgg cacgctcgaa agcactggga gcaagcgggg cacgccctcg
      601 tccgctcact cgagcccggt gagacctgga agtactgctt tcccgatcac tttctcatgg
      661 agtgagcaac accgattcct ccaggggggg cgttcgggcc ggctgaccga cgtcggctag
      721 cgtgattccc cgcatgaaag tatcaatgac gatcaacggg cgggcggtga ccgccgacgt
      781 ggagccgcgt actctgctcg tccacttcat acgggagcag atgaggctca ccggcaccca
      841 tgtcggatgc gagaccgggt actgcggcgc ttgcacggtc catctcgatg gcaaagcggt
      901 caagtcgtgc acgatgttcg ccgttcaggc cgaaggcgcg gacataaaga ccatcgaggg
      961 tgtggcaagc ggtggagcac ttcaccccgt ccaggaaggg ttctgggagc gacacggtct
     1021 ccagtgtggg ttctgcacgc cgggcatgat catgtcggca atcgccctcc tcgacgagaa
     1081 cgacaacccc tccgaggccg agattcggca tggcatcgaa ggaaacctct gtcgctgcac
     1141 cggttatcac aacatcgtcc gggccgtcga gtacgcggcg gcgaagatgc gcggcgagga
     1201 gccaccaccc cccgactggg agcggacctc ctgatgtacc cccgacagtt cgactacatc
     1261 gctccgacgt cgatcgagga ggctcttgcc gccttgggcg aggactcgaa ggtcatggca
     1321 ggcggtatga gtctgattcc gctgatgaag ctgaggctgt tctcccctgg cctcgtcgtc
     1381 gacataggac gcatacccga cctcgacggc gtcgccgagt tcggcgatca catcgagatc
     1441 ggagctttgg tgcgacatgc cacgacggcg actcatccct tgatcaggaa tcacgcccct
     1501 gccctggcca ccgcggcttc attgacaggt gacgttcagg tgcgcaatcg cggcaccacc
     1561 tgcggcgcgt tcgcccactc cgatgtggct gcggatcagc ctgcgggagc cttggcatgt
     1621 ggagcggtca tgatcgctcg ctcctccgag ggaactcgag aaatacccgc cgccgacttc
     1681 ttcatcgact ccctcacatc ggccctcgaa ccggacgaga tcctgatatc ggcccgcttt
     1741 cccaaagtcg gacccggtga aggttcggcg tacgacaagc tgggacgtcg cggtggccac
     1801 tctgactatg cggttgccgg agcggccgcc tgggtcagga cgtcaaacgg ctcgatcacc
     1861 gacgcccggg tggccgtgac cggagtcggc acaaagccac agctgacagg agccgtcgcc
     1921 gaggctctga tcggaaccga cggctcggac tccgccatcg aggcagccgc cgagaaggca
     1981 gccgacgaca tcaccgtcct cgaggacctg tacggctcag aggagtacaa gacacatctg
     2041 gccaaggtct ttgcgggccg ggcggtgaaa gcggcgctcg ccaacgtctg atcgatctga
     2101 tcagacaggc tcgttggagc gtttctgagc cgtttccgag cccggcccct cgacccggtc
     2161 tccggcactc cctcgactcg acgcggctgt gatcgcccag atgtatccgc ccaataggag
     2221 accgatcacg acgacggaga gcagatcgag gccgacgagg aaaaacgcaa gcgcagcaat
     2281 caccacacca ccgaacagga gagccccggt gtgcggcgca atccaccgat cgagccggct
     2341 gccgtcagga ccggtctcgg tccattcctc gatcgtgcgg ttgagtgtga cgaaccactg
     2401 tggtcgcccg gccagatagg ccacgaccgc gacaaggact gctgcgaccg ccaggatcag
     2461 ctggatcgcg cgcagacccg agagcgcatc tctcacaaga ccctcagcca gcaccgagcc
     2521 acgcggatcg acgatttgcc caacgaccgc ttcctgcagt cgtcggatgg cgacgctggc
     2581 aatcacgatg gcgacgaaga cgccgaggcc gagatggatc gccgtcttcc gacgattcgg
     2641 ggaagcgaca agtgtcagca ccaggagaac gagggtgaga gcgagagttg cccagacgag
     2701 gcggtcgagt tggacagcca cggtctggac ctctgccagg gcactctcag acatcaccgt
     2761 gacctggccg aagtcctcgg ggagactcgc ctgaagggcg gcagccaact gatccttgcc
     2821 ctcggacaac cggtccgata tcgcatcggg gaggtcgaag tcgggcagta ccgctcgaac
     2881 atcgtccgca acccgccgca gggcctcccc tatgaacggg atcagattga gtcgcacctc
     2941 accggcgtcg atgtagacgt tggggaggga agcgagatcg tct
//
LOCUS       DASUTA010000164         2201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_569685, whole genome shotgun sequence.
ACCESSION   DASUTA010000164 DASUTA010000000
VERSION     DASUTA010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569685"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..896
                     /gene="pstB"
                     /locus_tag="VFZ80_03305"
     CDS             138..896
                     /gene="pstB"
                     /locus_tag="VFZ80_03305"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX6286488.1"
                     /translation="MSVYYGDFLAVADVNLEFGTNQISALIGPSGCGKSTVLRSLNRM
                     NDLVATARTEGEVYYHGKNIYAEGVDPVEVRRTIGMVFQKPNPFPKSIFENIAWGAKI
                     NGFRGSKAETEDLVQEALQGAALWDEVKDKLNESGLSLSGGQQQRLCIARAIATKPDV
                     ILMDEPCSALDPIATLRIEELMLELKEDYSIVIVTHNMQQAARVSDKTAFFNIELSES
                     GMRTGRLVEYSATDQLFTSPKEKDTEDYITGRFG"
     gene            893..1600
                     /gene="phoU"
                     /locus_tag="VFZ80_03310"
     CDS             893..1600
                     /gene="phoU"
                     /locus_tag="VFZ80_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX6286489.1"
                     /translation="MTDTADTNIHFHSELDELERKVLSMVDRAEQMVEMAVQAVTTDD
                     LDLAGRVIELDDGIDATYNEVHQDWTSLMARHQPLGSDLRRMTSLIQLNVTFERMGDQ
                     CVNIAKIAQFNEGLPRVERICEQIREMGDLVRPMIRTAIEAYVRGDVDEARLLPAMDQ
                     PVDRLNANMYKETVAVGANPQLLEWATKMLMVSRALERVGDQAVDFGEQTAYLITGER
                     AEFVEDGMPVATDDDHD"
     gene            1584..>2201
                     /locus_tag="VFZ80_03315"
     CDS             1584..>2201
                     /locus_tag="VFZ80_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6286490.1"
                     /translation="MTTTTELRTQRILIIEDEPGLAESVRYALETEGFDVTVASSGFS
                     GLEAARMLSPDLVLLDLMLPEMSGLDVCRQIRLSSDVPIVMLTAKDSEADKVTGLELG
                     ADDYMTKPFSMRELVARVRAHLRRAAKSGVLADSNEVLRGGPIELDIDAHITRVGGDE
                     VELRPKEFELLESLMRRKNRLAARHTLIDEVWGPSYFGDTKTLDVH"
BASE COUNT          453 a          688 c          701 g          359 t
ORIGIN      
        1 ccggaatgac ccaagagacc tcgatcgaag gtagaacgag aaacgtgagt gacgagcgcc
       61 taggcacaga gcaggagact ccggtcgaaa ccgagaccga gatcgcacga gttccgggtg
      121 tcatcgagac cacccacatg agcgtctact acggcgactt cctcgccgtg gccgacgtga
      181 acctcgagtt cggcaccaac cagatatcgg cgctcatcgg cccatcgggc tgtggcaagt
      241 cgaccgtgct caggtcactc aaccgcatga acgacctggt ggccaccgcc cggaccgagg
      301 gtgaggtcta ctatcacgga aagaacatct acgccgaggg agtggacccg gtagaggtga
      361 gacgcaccat cgggatggtc ttccagaagc cgaacccctt ccccaagtcg atcttcgaga
      421 acatcgcctg gggggcgaag atcaacggct tccgcggcag caaggccgag accgaggacc
      481 tggtccagga ggcactgcaa ggagccgccc tatgggacga ggtcaaggac aagctgaacg
      541 agtccggtct ctcgttgtcc ggcggccagc agcagcgtct ctgcatcgcc cgcgccatcg
      601 ccaccaagcc cgacgtgatc ctcatggacg agccgtgctc ggccctcgac cccatcgcca
      661 cgttgcggat cgaggagctg atgctggagt tgaaggagga ctactccata gtcatcgtga
      721 cccacaacat gcagcaggcg gcgcgggtca gcgacaagac ggccttcttc aacatcgagt
      781 tgagcgagtc ggggatgcgg accggccgtc tcgtcgagta cagcgccacc gaccagctct
      841 tcacctctcc gaaggaaaag gacaccgagg actacatcac aggtaggttc ggatgacgga
      901 cacagccgac accaacatcc atttccactc cgagctcgac gagctcgaac gcaaggtcct
      961 ttcgatggtc gaccgggccg agcagatggt cgagatggcc gttcaggcgg tgaccaccga
     1021 cgacctcgat ctcgccggtc gggtgatcga gctcgacgac ggcatagacg ccacctacaa
     1081 cgaggtccat caggactgga cgtcgctcat ggcgcggcac cagcccctgg gctcggacct
     1141 ccgtcgcatg accagtctca tccagctcaa cgtgaccttc gagcggatgg gggaccaatg
     1201 cgtcaacatc gccaagatcg cgcagttcaa cgagggtctg ccccgggtgg agaggatctg
     1261 tgagcagatc cgggagatgg gagacctggt gcgccccatg atccggacgg ccatcgaggc
     1321 ctacgttcgg ggcgacgtcg acgaggccag gcttctcccc gccatggacc aacccgtcga
     1381 tcggctcaac gccaacatgt acaaggagac cgtcgcggtc ggggccaacc cccagctatt
     1441 ggagtgggcc accaagatgc tgatggtgtc acgtgctttg gagcgggtcg gtgatcaggc
     1501 ggttgacttc ggggagcaga cggcttacct gatcacgggc gagcgggcag aattcgtgga
     1561 ggacggcatg cctgttgcga ccgatgacga ccacgactga gctacgcacc cagcgcatcc
     1621 tcatcatcga ggatgagccc ggcctcgccg agtcggttcg ctatgccctg gaaaccgagg
     1681 gtttcgacgt cacggtggct tcgagtggtt tcagcgggct ggaggcagcg cgcatgttgt
     1741 caccggacct ggtcctcctc gacctgatgc tcccggagat gtcggggctc gacgtgtgcc
     1801 gtcagatccg tctctcctcc gacgtcccca tcgtcatgct gacggcaaag gactccgagg
     1861 ccgacaaggt gacggggctc gagctcggcg ccgacgacta tatgaccaag ccgttctcga
     1921 tgagagagct cgtggcccgg gtgcgcgccc acctccgccg cgccgccaag tcgggggttc
     1981 tggcggattc gaacgaggtg cttcgtggcg ggccaatcga gctggacatc gatgcccaca
     2041 tcacgcgggt tgggggcgac gaggtggaac tgcgtcccaa ggagttcgag ctcctcgagt
     2101 ctttgatgag gagaaagaac cgtctcgctg cccggcacac cttgatcgac gaggtctggg
     2161 gcccgagcta cttcggtgac accaagaccc tcgacgtgca c
//
LOCUS       DASUTA010000165         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1087702, whole genome shotgun sequence.
ACCESSION   DASUTA010000165 DASUTA010000000
VERSION     DASUTA010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087702"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VFZ80_03320"
     CDS             <1..774
                     /locus_tag="VFZ80_03320"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEX6286491.1"
                     /translation="FVPTAIIDASLAFVAFTLAASGTYLINDTIDAPVDRLHPTKRHR
                     PIAAGVISSRLAITMALLLLAASFLPATLIGRGEFTATLIAYVALTVSYSLWLKQIAV
                     VDLVVVASGFGLRAVGGAFSIDVPVSQWFLIVTFFGALFVVAGKRFGEQQSLGAAAEK
                     HRPTLSAYADFYTQHVMTLSSGIALIGYSLWAHDHGYLSDWWFLASLMLLVMTLLRFA
                     LLVHAGASDDPVEIAWNDRPLRVLALLLSVLVVGGIQFG"
     gene            complement(806..1555)
                     /locus_tag="VFZ80_03325"
     CDS             complement(806..1555)
                     /locus_tag="VFZ80_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6286492.1"
                     /translation="MSPVDSFGRSQTVLVLGGNSDLGQAIARRMVDDGARTVILAGRD
                     PERPTDPPIDADTDHRYFDAADTASHDKFFNEVFSDHPNIDAVVIAFGVLHSQGSVDQ
                     RPELAVEMAQVNYVGAASALLHSERRLRERGGGQLVVLSSLAGLQPRPSNFSYGSTKA
                     GIDFLARGLARTSQRGNVHVLVVRPGFVHTAMTADLPSRPFAVGPEDVADAVVEALSR
                     RAGVVFVPAILKWVMWVLRLLPYPIVARLDR"
     gene            complement(1555..>2283)
                     /locus_tag="VFZ80_03330"
     CDS             complement(1555..>2283)
                     /locus_tag="VFZ80_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenylphosphoryl-beta-D-ribose oxidase"
                     /protein_id="HEX6286493.1"
                     /translation="LADADSQHRYSVAWLDLTRRGKGRGSVMGGDHARSVEAPAGLRD
                     ADLAGPTIRIPGATPALVNRVTVNVFNQIWYAKAPRNVSRGVESMGSFIYPLDGLTAW
                     NRLYGDSGFLQYQFVVPYGEESTLVAVAETLVATPTPVSLAVLKRMGDSVGGYLSFPM
                     PGWTLAVDMPLGDPRLGAVLDRCDERVADAGGRVYLAKDSRLRRDIAHEMYPDLAKWR
                     EVQAKLDPARRLRSNLSERLELIA"
BASE COUNT          378 a          752 c          737 g          416 t
ORIGIN      
        1 ttcgtgccca cggcgatcat cgatgcctcc ctggccttcg tggccttcac cctcgccgcc
       61 agtggcacct acctgatcaa cgacacgatc gatgcgcccg tggatcgtct ccacccgacc
      121 aaacggcatc gtcccatcgc ggctggggtc atctccagcc gcttggcgat caccatggcg
      181 cttcttctcc tggccgcgtc gttcctcccc gcaaccctga taggtagggg agagttcacc
      241 gccacgctga tcgcctacgt cgccttgacg gtttcctatt cgctttggct gaagcagatc
      301 gcggtcgtcg atctcgtcgt ggttgcctcc ggattcgggc tgcgtgccgt gggaggagcc
      361 ttctcgatcg acgtgccggt ctcgcagtgg tttctcatcg tcaccttctt cggggcactc
      421 ttcgtcgtcg ccggcaagcg attcggtgag caacagagtc tcggcgccgc ggcggaaaag
      481 caccgtccca cgctctctgc ctatgccgac ttctacaccc agcacgtgat gaccctgtcc
      541 tccgggatcg ctctcatcgg gtacagcctc tgggcccatg accacggcta cctgtccgac
      601 tggtggttcc tcgcctccct gatgctcctg gtgatgacct tgctcaggtt cgcactgctg
      661 gtccacgccg gggcttcgga cgatccggtg gagatcgcat ggaacgaccg accacttcgg
      721 gtgttggcgc tcttgctctc cgtcctcgtc gtcggtggga tccagttcgg ttgaccaccg
      781 cccgccccct gaacgaggag cgtggtcagc ggtcgagcct ggcgacgatc gggtagggga
      841 ggagtcgcag cacccacatg acccatttca ggatggcggg cacgaacacc acgcccgccc
      901 gacggctgag agcctcgacc accgcgtcgg ccacgtcttc ggggccaacc gcaaacggcc
      961 gggatggaag gtcggcggtc atggccgtgt gaacgaatcc cggtcggacc accagcacat
     1021 ggacgttgcc tcgctgcgag gtccgggcga gaccacgggc cagaaagtcg attccggcct
     1081 tcgtcgatcc ataggagaag ttggacgggc gtggctgaag gccggccaac gaagatagga
     1141 ccaccagttg accgccaccg cgttcgcgga gtcggcgctc ggagtggagc agagctgacg
     1201 ctgctcccac atagttgacc tgggccatct cgactgcgag ctcgggccgc tgatcaaccg
     1261 acccctggct gtgcagcacc ccgaaagcga tgaccacagc gtcgatgttg gggtggtccg
     1321 agaagacctc gttgaagaac ttgtcgtggg aggcggtgtc ggcggcgtcg aagtagcgat
     1381 ggtcggtatc ggcgtcgatg ggggggtccg tgggacgctc ggggtccctg ccggccagga
     1441 tcaccgtgcg ggcaccatcg tcgaccatgc gtcgcgcaat cgcctggccg aggtcggagt
     1501 tgccgccgag gacgaggacc gtttgtgatc gtccgaacga gtcgaccggg gacatcaggc
     1561 gatcagctcg agacgctcgg acagattgga tcggagtcgc cgcgcagggt cgagcttggc
     1621 ctgcacctcg cgccacttcg ccagatcggg gtacatctca tgggcgatgt cacggcggag
     1681 acgcgagtcc ttggccagat agacccgacc tccggcatcg gcaacacgct cgtcgcatcg
     1741 gtccagcact gctcccaggc gcgggtcgcc gagaggcatg tcgacggcga gggtccagcc
     1801 gggcatcggg aacgagaggt atccgccaac cgagtcgccc atccgtttca ggaccgcgag
     1861 cgagaccgga gtcggtgtcg ccaccagcgt ctcggccacg gcgaccaggg tgctctcctc
     1921 tccatagggg accacgaact ggtactgcag gaaccccgag tcgccataga gacggttcca
     1981 ggcggtgaga ccgtccagcg gatagatgaa ggagcccatc gactcgacgc cccggctgac
     2041 gttgcgtggc gccttggcgt accagatctg attgaagaca ttcaccgtca cccgattgac
     2101 cagtgcgggc gtggcgccgg ggatccggat cgtgggcccg gcaaggtccg cgtctcttag
     2161 cccggcgggt gcttccacgg atcgagcgtg atcacctccc atgacgctcc cacggccctt
     2221 tcccctccgg gtcaggtcca gccatgcgac cgagtatcga tgttgcgaat cggcgtctgc
     2281 cag
//
LOCUS       DASUTA010000166         2849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1865622, whole genome shotgun sequence.
ACCESSION   DASUTA010000166 DASUTA010000000
VERSION     DASUTA010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1865622"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFZ80_03335"
     CDS             complement(<1..196)
                     /locus_tag="VFZ80_03335"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX6286494.1"
                     /translation="MKPVIGITSWKRHLDTFYGPDTLQTLAAFYTDSIASAGMSPVMF
                     PSSLDPGDAERLVSLVDGVLL"
     gene            complement(193..1074)
                     /locus_tag="VFZ80_03340"
     CDS             complement(193..1074)
                     /locus_tag="VFZ80_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX6286495.1"
                     /translation="MPEYRRILLHDREVQTHVEDEELVTSDGDRVQIEDAVHLPPVEP
                     TKIIATHLTYRSRVEEFMTKLPPAPTYFHKPTTSLNSHMGDVVRPGRCKYLNYEGEIA
                     IVIGHRAFDISREEAAHHIAGYTIANDYGLHDFRDTDAGSMLRVKGSDTLCPIGPGLV
                     TDWDFRDKSIRTLVNGEVRQSANTSEMAWDMHYLVADLARTITLLPGDIILSGTPAGS
                     RPVEPGDVVTVEVEGLGALTNRIVEGQTAVRSELGAQPSESEEVISTALGGDWEYRGM
                     RAPRGPGAKHYESEVSE"
     gene            complement(1094..>2849)
                     /locus_tag="VFZ80_03345"
     CDS             complement(1094..>2849)
                     /locus_tag="VFZ80_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286496.1"
                     /translation="GNRSGSSPRSLVVVLPEGAPDRERPKRQSRSVILAIAALGMALG
                     AIVLGSLTAPDPEAAPESTTTTATDDVEQPIDFEDFTIAEIERGTPFDWVRAHSVSDG
                     FPLALFEHEGWNYLFATDVPSFAGRVEGGLRAWRSEDGVEWQSLGQVIGSEHSVNNAE
                     STLQGLVAVESGTGDGEFALWRSDDGIEWWREVIETGGPSAGTRYGHAIGGNDHILMV
                     TATPSVDLFGQLEDRLPDVLEGGLDITRLPWSPEFTDRELLIVVWGPLGFPIVEISGD
                     ELGLTDEEMADLERAWNGPGSDLEAWVSAEPGHWFQSELPDASWINSITPTPNGEILA
                     AGWGHQSNAIWSSIDGVNWGKIETFTGPDEIENWNGLLVGPYRSGSADILTSVDGHQW
                     EPIGLREHLPGQLQWNIAAMATGPGGVGATIDGWSSSTFVPDDEPVVLTDGEATLSIN
                     FSTGSYQVESGEIVRRWSMNSSLQPEGVILDLAGQRVTFHAESGEELASFTLGQLTDA
                     ESEFWNATAMEDRHNGFVFSRDGEDWTIQDAGEAMQSYIMMLEVTDSHVIAAVASLEG
                     RFSPQTSPGFEIWSAPIP"
BASE COUNT          488 a          882 c          916 g          563 t
ORIGIN      
        1 agagcaggac tccgtcgacg agggagacca ggcgctcggc atctccggga tcgagtgacg
       61 atgggaacat gaccggactc atcccggcgc tggcgatcga gtcggtgtag aacgcggcga
      121 gtgtctgcag ggtgtccggc ccgtagaagg tatcgaggtg tcgtttccac gaggtgattc
      181 ctatcaccgg cttcattcgc tgacctccga ctcgtagtgc ttggcccccg ggcctcgggg
      241 tgctcgcatc ccacggtatt cccaatctcc gccgagagcg gtggagatga cctcctcaga
      301 ctcgctgggc tgggcgccga gctcggagcg gaccgcggtc tgaccctcga cgatccggtt
      361 ggtgagagct ccgagccctt ccacctccac ggtcaccaca tcacccggtt ccacgggccg
      421 tgagcctgcc ggggtgccgc tgaggatgat gtcaccgggg aggagcgtga tcgtgcgcgc
      481 cagatcggcg accaggtagt gcatgtccca ggccatctcg gatgtgttgg ccgactgcct
      541 cacctcacca ttgaccagag tgcggatgga cttgtcgcgg aagtcccaat cggtcacgag
      601 gccagggccg atcgggcaaa gggtgtcgga gcctttcacc cgcagcatcg agccggcgtc
      661 ggtgtcacgg aaatcgtgca acccgtagtc gttggcgatg gtgtaccccg ctatgtggtg
      721 tgccgcctcc tctcgtgaga tgtcgaaggc ccggtgtccg atgacgattg ctatctcacc
      781 ttcgtagttg aggtacttgc accgtccggg tcgaaccaca tcacccatgt gggagttgag
      841 tgaggtggtc ggcttgtgga agtaggtggg agcaggggga agcttcgtca tgaactcctc
      901 gacccggctc cggtaggtga gatgggtggc gatgatcttg gtcggctcca cgggtgggag
      961 gtgcacggcg tcttcgattt ggacccggtc tccatcgctg gtcaccagct cttcgtcctc
     1021 gacgtgtgtc tggacctcgc gatcgtgaag aaggattcgt cggtactccg gcatcggatc
     1081 tgacgctaac ccgtcacggg atcggagcgg accagatctc gaaaccagga gacgtctggg
     1141 gtgagaatcg cccttccaga gaagccaccg ccgctatcac gtgagagtcg gtgacctcca
     1201 gcatcatgat gtaggactgc atcgcctcgc cggcatcctg gatggtccag tcctccccgt
     1261 ctctgctgaa cacgaatccg ttgtgccggt cttccatcgc cgtggcattc cagaactcgg
     1321 actctgcgtc ggtgagctgt cccagggtga acgaggcgag ctcttcaccg gactccgcgt
     1381 ggaaagtcac ccgctggccg gccaggtcga gaatgactcc ttccggctgc agcgaggagt
     1441 tcatcgacca gcgcctgacg atctcaccgg actcgacctg ataggagccg gtcgagaagt
     1501 tgatcgacag tgtggcctct ccgtcggtga ggacgacggg ctcgtcatcg ggaacgaatg
     1561 tggagctcga ccagccatca atggtggcgc cgacgccacc ggggccagtg gccatcgcgg
     1621 cgatgttcca ctgaagctgc ccgggtaggt gctccctcag gccgatgggc tcccattggt
     1681 gaccgtcgac ggaggtgagg atgtccgctg atccggaccg gtaagggccg accaggagcc
     1741 cgttccaatt ctctatctcg tctggaccag tgaaggtctc gatcttcccc cagttgaccc
     1801 catcgatgct cgaccagatg gcgttgctct ggtggcccca tcccgcggcg aggatctctc
     1861 cgttcggtgt cggggttatc gagttgatcc aggaggcgtc cgggagctcg ctctgaaacc
     1921 agtggccggg ctctgccgat acccaggcct cgagatcgga accgggtccg ttccatgccc
     1981 gctcgaggtc ggccatctcc tcgtcggtca ggcccagctc atcgcccgag atctcgacga
     2041 tagggaaccc cagtggcccc cagaccacga tcaagagctc tctgtcggtg aactcggggc
     2101 tccagggaag ccgggtgatg tcgagcccac cttccagcac atcgggaagt ctgtcttcga
     2161 gctgcccgaa taagtcgaca ctcggcgtgg ccgtgaccat gaggatgtgg tcgtttcctc
     2221 cgatggcgtg gccatatcgg gtgccggcgc tcgggccacc agtttcgatg acctctcgcc
     2281 accattcgat gccgtcgtcg gagcgccaca aggcaaactc accatcgccc gtgccgcttt
     2341 cgacggcgac cagcccctgg agagtcgatt cggcgttgtt cacgctgtgt tcgctgccga
     2401 tcacctggcc gagtgattgc cactcgactc catcttctga tcgccaggcc cgcaagccgc
     2461 cttcgacacg gccggcgaag ctgggcacgt ctgttgcgaa taggtagttc cagccctcat
     2521 gctcgaagag agccaagggg aagccgtcgc tgaccgagtg ggcacggacc cagtcgaatg
     2581 gcgtgcctcg ttctatctcg gcaatggtga agtcctcgaa gtcgatcggc tgctcgacat
     2641 cgtcggttgc cgtggtcgtg gtggactctg gcgccgcttc tgggtccggt gcagtgagcg
     2701 agccgagaac gatagccccc agggccatgc ccaaggctgc aatggcgaga atgaccgacc
     2761 ggctctgacg ttttggcctc tctcggtctg gagctccctc gggtaggacc acgaccagag
     2821 aacgtggtga ggatcccgac ctgttcccg
//
LOCUS       DASUTA010000167         3693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_181470, whole genome shotgun sequence.
ACCESSION   DASUTA010000167 DASUTA010000000
VERSION     DASUTA010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181470"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VFZ80_03350"
     CDS             complement(<1..565)
                     /locus_tag="VFZ80_03350"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6286497.1"
                     /translation="MDLFLIRHGQPDWAPGLIARNDPGLTDLGREQAERVGHRFSALN
                     GVDELWSSPMNRSMETTGPVETSLGIDREVYDWLKEIQNPPEWEGSPVEVIERGFEQA
                     NFREMNEMWDGLPGGESFRDFHERVIAGLIETMDSHGVHPMAPDSRHLWNVDDPDKRV
                     VIVAHAGTNALILGHLLGLDPVPWDWDR"
     gene            620..1168
                     /gene="pyrE"
                     /locus_tag="VFZ80_03355"
     CDS             620..1168
                     /gene="pyrE"
                     /locus_tag="VFZ80_03355"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX6286498.1"
                     /translation="MAGNALVDHLVKHALRTDGPFTLRSGASSDWYVDARQTTFDGAG
                     ARAVGEAVLAVLDERVAAVGGMTMGADPIAVAVAMTAAAAGRDLKAFSIRKEPKDHGT
                     GGRLVGPVDPSMPVAVLEDTTTTGGAAVEAARLLLDEGFEVVQAIALVDRSEGKARDN
                     FAELGISHVALVLPSDLGVVSA"
     gene            1165..1752
                     /locus_tag="VFZ80_03360"
     CDS             1165..1752
                     /locus_tag="VFZ80_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286499.1"
                     /translation="MTVVVRRSALKMWLLAMAGIPLLIISLDVLTERRITRWLTDLIF
                     LPEDVQIYEPRDVIFAWVMFLFGAFLVLWGLRELFVPAKVVECRDEGLALRINGPFQP
                     PSVIPWENIQDVGGADMEDEGDEIVLFVVGVFSRDGLPENPWGARWVTENELGVLAED
                     WGDDPQVVAENVADYAVDAVKRKQSEETSKVWQET"
     gene            1752..2510
                     /locus_tag="VFZ80_03365"
     CDS             1752..2510
                     /locus_tag="VFZ80_03365"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX6286500.1"
                     /translation="MLVGAHTPSEHPLEEAAARKADLVQIFLSNPQGWKKPKPRADAE
                     ELRNSPIPVVVHAPYLINVCADNNRVRIPSRKILQETCDAAAEIGAEAVVVHGGHVTG
                     EGPEEEGIVRWRKALDTVDTDRTILIENTAGGDKAVARRVEWLKRLWEEIGSYGVGFV
                     LDTCHAWAGGEPFDELIERVLDATGRIDLVHANDSKDAFDSRRDRHQNLGAGEIPKED
                     LASVILAAEAPVVVETPGGVAEQGADIEWVRNLV"
     gene            2579..2827
                     /locus_tag="VFZ80_03370"
     CDS             2579..2827
                     /locus_tag="VFZ80_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286501.1"
                     /translation="MAVLFADRTDRALDLLELVELAWHDVYGEVTPSEDVIDDVLILS
                     EGSLRALIRYARLAVEDWRDVRVEADKLRAGRAAADEA"
     gene            complement(2828..2980)
                     /locus_tag="VFZ80_03375"
     CDS             complement(2828..2980)
                     /locus_tag="VFZ80_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286502.1"
                     /translation="MTTTQVTMESIQSQIAHALGTANTRTSDEHLELLADLLAQAFNA
                     VRVEEN"
     gene            3129..>3693
                     /locus_tag="VFZ80_03380"
     CDS             3129..>3693
                     /locus_tag="VFZ80_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX6286503.1"
                     /translation="MSKVTAPGIRSRKGGPKIAVVTAYDFPGAVIADRAGIDIILVGD
                     SVANVVHGMDTTLEIGLDEIVLHTRAVKRAKPSALVVADMPWLTFHLGEVDAVRNAGR
                     LVREGGAEAVKLEGGRKRLPVIEAILDAEIPVMGHVGLTPQSVHTMGGFKVQGKVVDA
                     AREMIEDAKALSDAGCFSIVIEGVPDIL"
BASE COUNT          677 a         1118 c         1211 g          687 t
ORIGIN      
        1 aacggtccca gtcccaggga accgggtcga gaccgagcag atgccccagg atcagggcat
       61 tggtacctgc gtgagccacg atcaccaccc gcttgtcagg gtcgtccacg ttccacaagt
      121 gccgcgagtc gggagccata gggtgaacgc cgtgcgagtc cattgtctcg atgagcccgg
      181 cgatgaccct ctcgtggaag tcacggaaag actcgccgcc tggcagcccg tcccacatct
      241 cgttcatctc ccggaaattg gcctgctcga agccgcgctc gatcacttcg acaggcgatc
      301 cttcccattc cggcggattc tggatctcct tcagccagtc gtagacctcg cggtctatgc
      361 ccagggacgt ctcaacaggc ccggtggtct ccatcgagcg gttcatgggc gaggaccaga
      421 gctcgtcgac tccgttgaga gccgagaagc gatgacccac gcgctcggcc tgctcccgcc
      481 cgagatcggt gagaccggga tcgttgcggg ctatcaggcc gggtgcccag tcgggctgcc
      541 cgtgccggat cagaaacaag tccatcagtc gacaacctat acgttccagt tcccttcaac
      601 tccccaatac gatggtgacg tggctggcaa cgccctcgtc gatcatctcg tcaaacatgc
      661 cctgcgaacc gacggaccct tcacgcttcg cagcggggcg tcttcggatt ggtatgtcga
      721 tgcccgtcag accacgttcg acggtgccgg ggcccgagca gtgggcgagg cggtcctggc
      781 tgtcctcgat gagagagtcg ccgccgttgg tgggatgacc atgggggccg acccgattgc
      841 agtggctgtc gccatgaccg ctgcggccgc gggccgcgac ctcaaggcgt tctcgattcg
      901 taaagagccc aaggaccacg gcaccggcgg ccgattggtc ggaccggtgg acccctccat
      961 gcccgtggcc gtgctcgagg acaccacgac cacagggggc gctgcagtcg aggcggcgcg
     1021 tttgctgttg gacgaggggt tcgaggtcgt ccaggcgatc gccttggtgg atcgatcgga
     1081 ggggaaagca cgcgacaact tcgccgagct ggggatcagt catgtcgctc tggtccttcc
     1141 ttccgatctc ggagtggtgt cggcatgacc gtcgtcgtgc gcaggtcggc cctcaagatg
     1201 tggctcctgg ccatggccgg gatcccgctt ctcatcatct ctctggacgt tctcactgag
     1261 cggaggatca cccgatggct caccgatctc atattcctcc cagaggacgt ccagatatac
     1321 gagcccaggg acgtcatctt cgcctgggtg atgtttctct tcggcgcctt tctcgtcttg
     1381 tggggactgc gtgagttgtt cgtccctgcc aaggtcgtcg agtgccgcga cgaaggcctg
     1441 gcccttcgaa taaatggccc gttccagcca cccagcgtca taccttggga gaacatccag
     1501 gatgtgggtg gggctgacat ggaagacgaa ggtgacgaga tcgtcttgtt cgtcgtcggt
     1561 gtgttcagca gagacggcct ccccgagaac ccgtgggggg cgcggtgggt cacagagaac
     1621 gagttggggg ttctcgccga ggactgggga gacgaccctc aagtggtggc cgagaacgtt
     1681 gccgattacg cggtcgatgc agtgaagcgg aagcagtccg aggagacatc caaggtctgg
     1741 caggagacat gatcctggtc ggagcacaca caccctcgga gcaccctctg gaggaggcgg
     1801 cggctcgaaa ggccgacctg gtgcagatct tcctctccaa cccccagggt tggaagaagc
     1861 caaagccacg cgccgacgcc gaggagctgc gaaattcgcc catacccgtc gttgtccatg
     1921 ctccctacct gatcaacgtc tgcgccgaca acaaccgggt gcggataccg agcaggaaga
     1981 tcctccaaga gacatgtgac gcggcagccg agatcggtgc cgaagccgta gtggtccacg
     2041 gcggacacgt gaccggggag ggccctgaag aggaaggcat cgttcggtgg aggaaggctt
     2101 tggacacggt ggacaccgac aggacgattc tcatcgagaa caccgcaggg ggagacaagg
     2161 ccgttgctcg acgcgtcgag tggctgaaga ggctttggga agagatcggc tcatacggcg
     2221 tcggcttcgt cctcgatacc tgccacgcct gggctggtgg tgagccgttc gatgagttga
     2281 tcgagcgggt cctcgatgcc actgggcgga tcgatctggt tcacgccaac gactcgaaag
     2341 acgctttcga ctcgagacgc gatcgtcacc agaacctggg cgccggcgag atcccgaagg
     2401 aagatctcgc atcggtgatt ctggctgcgg aggctcctgt cgtggtggag actcccggtg
     2461 gcgtcgccga gcagggcgcc gacatcgagt gggttcggaa tctggtgtag tcagtgccgg
     2521 gaaaccagcg ccaatgccag taggccgcgc cgcgcgacgg gcggagcgtc gcacgaaggt
     2581 ggcggtgctc ttcgccgatc ggaccgaccg cgccctcgac cttctcgaac tggtcgaact
     2641 ggcctggcat gatgtctatg gagaggtgac gcccagcgag gatgtcatcg atgacgtcct
     2701 catactcagt gagggcagtc tgcgtgcgct catccggtat gcccgtctcg cggtggagga
     2761 ctggcgcgac gtcagggtcg aagctgacaa gctgcgggcc ggtcgggctg cagctgacga
     2821 ggcctgatca gttctcctcg acccgcaccg cgttgaaggc ctgggcgaga agatcagcca
     2881 gcagctccag atgctcgtcg ctggtcctgg tgttggctgt cccgagggcg tgagcgatct
     2941 gagactggat ggactccatc gtcacttgag tggtggtcat gaacatttcg acgtactggg
     3001 gtcttacccg gttcctctcc cttgagccct tctgattccc gaagcagtcg ggaccgaaac
     3061 ctctgctgac cctcaggaat ggttgggctg tgcccgtggt ttccctgcgc aggtaccatt
     3121 tctgattgat gagcaaggta accgcccccg gcatccgatc ccgcaagggt gggcccaaga
     3181 tcgccgtggt gactgcatac gacttcccgg gagcggtcat agccgaccgc gccggcatcg
     3241 acatcattct cgtcggagac tcggtggcca acgtcgtgca cggcatggac acgaccctgg
     3301 agataggtct cgacgagatc gtcctccaca ctcgcgccgt gaaacgtgcc aagccgagtg
     3361 ccctggtcgt ggcggacatg ccctggctca cgttccatct cggagaagtg gatgctgtgc
     3421 gcaacgccgg tcgactggtc cgcgaaggtg gggccgaggc ggtcaaactc gaaggcggcc
     3481 gaaagagact tcccgtcatc gaggcgatcc tcgatgccga gatcccggtc atgggtcacg
     3541 tcggactgac cccgcaatcg gtacacacca tgggtggttt caaggtccag ggcaaggtgg
     3601 tcgacgcagc gcgcgagatg atcgaggacg ccaaggctct ctcggacgcc ggatgcttct
     3661 ccatcgtcat cgagggagtg cccgacatcc tgg
//
LOCUS       DASUTA010000168         3533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1734939, whole genome shotgun sequence.
ACCESSION   DASUTA010000168 DASUTA010000000
VERSION     DASUTA010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1734939"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..405)
                     /locus_tag="VFZ80_03385"
     CDS             complement(13..405)
                     /locus_tag="VFZ80_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286504.1"
                     /translation="MRIITVVVLIIFTACSNDDLPYQTFTDADGVVRTQTDCPSAETQ
                     ISGGAPDGLPMKGQTELARVEELAAASLDSEVVPRNGEVWSQADGGAVTVEQVEDFMI
                     LITISDVSRCPDTPMFSNGVPIIYRVGD"
     gene            complement(509..1531)
                     /locus_tag="VFZ80_03390"
     CDS             complement(509..1531)
                     /locus_tag="VFZ80_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286505.1"
                     /translation="MIDPWSFESYGMFGGFELAVVSGAMSYTTTAEFVMEDVEVEIRS
                     DTAGRFSWHRLGGDPLDREVPSGDWIITPGGAWIESSGEWVQADTGDIGDAWAMALAP
                     WQLSLPGSVYTQVYGSLSGLDFAGCDEMEGRLVAVYRGGPDSVGAYFGEEAGAVVSGA
                     IEVWMDPKGFPMKVVTEATEIVDLSVPRSMDWALTALGTSIEMEIPDEISVATELWVP
                     LLAAGLPDRPCPEGATDGPCDLAVVVYGDGRWTVDDGYGQETGSIDLNVLQPLKDALS
                     NVEYSELGPLIRNGTCPAELDDQGTVVLFPSLPSYELYASCRPFDDNVAPYREALELI
                     AQVPLD"
     gene            2110..2721
                     /locus_tag="VFZ80_03395"
     CDS             2110..2721
                     /locus_tag="VFZ80_03395"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEX6286506.1"
                     /translation="MGTTIGFRGAWSAWLIASLALGLVILPAVVWLVLSGVQAANGDL
                     QVTMETTVSVESPVSLPDGWEVAGVLPVRVTMVDPPISQFLLLAGSRAGDFLLVVGVL
                     WLIQKVAASIRRGDPFHEANVRRLRWIGVLLLIGYPLAVVNGGFFTNWFFSNEHSPEL
                     ADGMVIGFPVISLGAILGGLCMLVLAEVFRHGIGLREDVEATV"
     gene            2727..2951
                     /locus_tag="VFZ80_03400"
     CDS             2727..2951
                     /locus_tag="VFZ80_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6286507.1"
                     /translation="MAPSEADYNIVVHLDELLASRGMTLTELADAVDITIVNLSILKN
                     GRARAIRFSTLSALCEFLGCQPGELLSYRS"
BASE COUNT          719 a         1070 c         1033 g          711 t
ORIGIN      
        1 cacaaagact gttcagtcgc cgacccgata gatgatgggg acgccgttgc tgaacatcgg
       61 cgtatcgggg catcgactga cgtcggagat cgtaatgaga atcatgaagt cctcgacctg
      121 ctcaaccgtc accgctccac catccgcttg gctccagacc tcgccgttac ggggcacgac
      181 ctcagagtca aggctggcgg cagcgagttc ctccaccctg gccagctcag tctgaccctt
      241 catcggaagc ccatcgggtg cgccccctga aatctgggtt tcagcgctgg gacagtctgt
      301 ctgggtcctg accactccgt cggcatccgt gaaggtctgg tagggcagat cgtcgttcga
      361 gcaggcggtg aagatgatca agacgacgac ggtgatgatt ctcatggcgg tgctcccgcg
      421 ctgaaacgct actgatcagg gaaccggttc ccgggtgcgg acagccccag atcgttcctt
      481 ctctcaaagt cagcgtgcta atcgcaagtc agtcgagggg gacctgcgcg atcaactcca
      541 aggcttccct gtagggggcc acgttgtcgt cgaatgggcg acaggaggcg tatagctcat
      601 agctgggaag agagggaaac aacaccaccg tcccctggtc gtcgagttcg gcgggacacg
      661 tcccattgcg gatcagtggg ccgagctcgg aatattcgac gttgctgagg gcgtctttga
      721 gaggctgcag aacgttcaga tcgatggatc ccgtctcctg cccgtacccg tcatcgaccg
      781 tccatctccc atcgccgtag acaaccactg ccaggtcaca gggcccgtcg gtggctccct
      841 ccgggcaggg acgatctgga aggccggccg caaggagagg aacccacagt tcggtcgcga
      901 cgcttatctc gtcgggaatc tccatttcga tagacgtgcc cagagcggtg agagcccaat
      961 ccatcgatct tgggacggag aggtcgacga tctcggtcgc ttcggtgacg accttcatcg
     1021 gaaagccttt cgggtccatc catacctcga tggcacccga gaccacagca ccggcctcct
     1081 caccgaagta ggcgccgacc gagtcggggc caccccgata cacggcgacc aaccggcctt
     1141 ccatctcatc gcacccggcg aagtcgaggc cagacaagct tccataaacc tgggtgtaga
     1201 cactgccggg tagggagagc tgccagggag ccagagccat cgcccaagca tcgccaatat
     1261 ctcccgtgtc ggcctgcacc cactccccag aactctcgat ccatgctcct cccggcgtga
     1321 tgatccaatc acccgacgga acctcccgat ccaagggatc accgcccagg cgatgccatg
     1381 agaaccggcc cgctgtatca gaacggatct cgacctcgac gtcttccatc acgaattccg
     1441 cagtcgtcgt gtaggacatg gcgcccgaca ccaccgccag ttcaaacccg ccgaacatgc
     1501 cgtaagactc gaaactccac ggatcgatca tctccgcagt tcgacagtca ctctcagagg
     1561 agctttccat aatcgtggtc ggcgcagagg tggggacagc cgttgaaccg ggcgacggct
     1621 gagtggtggc cggcgccgac gacccaatcg atgacatcgt cggtgaagcc tggctcgcta
     1681 ctgagctctc gcttctttca gtcgaaggac tcggctccgc acacgccacg agcgaaagag
     1741 tcaaaccgat cagcccgacc agaactcgag gtggaacatc acgaacgcgc ccggattccc
     1801 gaccccccaa catgaaaact cctaaccaac agaacagacg aatcggcagc aacgtacagg
     1861 cgccagcttg tccagcacac actccaactg gtcggagtcc ggaccgctag ggcgacccac
     1921 cacaagaccg aaactgacct ggaactccgg ccctactgtg atcggatgaa gagctgatga
     1981 cctgaagcac ctcgtcttct gagatgtggc cagaagtggt gacagcgcac tgagggtttg
     2041 tcacgtcttg acttgttatc gatagtcgtt attatcgtta ttcgataggg tcccgacgag
     2101 gagttgagaa tgggaacgac catcggcttt cgtggcgctt ggagtgcatg gctcattgcg
     2161 agcttggcct tggggctggt gatcctgccg gctgtggtgt ggcttgtcct tagcggtgtg
     2221 caggcggcga acggggacct ccaagtgacg atggagacca cggtgtcggt ggaatcacct
     2281 gtatctcttc ctgacgggtg ggaggtggcc ggtgtgctcc cggtccgcgt gacaatggta
     2341 gaccccccta tctcccagtt tctgctgttg gccgggtcga gggccggcga tttccttctc
     2401 gttgtcggtg ttctctggtt gatccaaaag gtcgccgcct ccatcaggcg gggcgacccg
     2461 ttccacgaag caaacgtgag gcgcttacgt tggataggcg tgctcttact gatcgggtat
     2521 ccattagcgg tggtcaacgg gggttttttc acaaactggt tcttctcgaa cgagcacagt
     2581 cctgagctcg ctgatggcat ggtcattggc tttccagtca tttctttggg cgccatcctc
     2641 ggcggactct gcatgttggt gcttgcagaa gtcttccgac acggcattgg gctgcgcgaa
     2701 gacgtcgaag cgacggtcta accgagatgg ctccttccga agccgattac aacattgtgg
     2761 tgcatttgga tgagttgctg gcgagtcgag gcatgaccct taccgagctg gcagatgcgg
     2821 ttgacatcac catcgtcaac ctctccatac tgaagaacgg ccgggctcgg gcgatccgct
     2881 tctccactct ctcggcccta tgcgagttcc ttgggtgcca gccgggcgaa ctgctctcct
     2941 acagaagcta gcgccagcga ttgtgtgccc aattggcttc tgctggacga atcgggggcg
     3001 accgggaccg acggtccaca acattggtga tcctgcccgg gtccgtcaga tcatccgcaa
     3061 cctgatctca aatgctctcc gctatggagg agaacaaatc gaagtctgcg tcaacagcga
     3121 cgcctccacc gctcaagagg tgggctgcaa tgggcggttc ctgactccag atgatgagcc
     3181 tcattccata accgtgtcga ttcaggagga gctcccgatg accggcttgc ccacgacgcc
     3241 attgattgtt gctgctctaa ccatcgcggc ggccggattt gtcttggtgg ctctccagac
     3301 tcataaccat tcagcacggg agaccagtcg caaataacta gcgcagatca ccagttctgg
     3361 agcgatatcg cccatatctg aaaccgcacg agatcggcac gtatcgctcg gggtgggggg
     3421 ctatggattc ccggctcccg gacacaccta aacccgggta agcactctcg cactgggggt
     3481 gactgcgcag gtcgtgtgcc tagcagtgtt cgagggagat cgggggattc tct
//
LOCUS       DASUTA010000169         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1606849, whole genome shotgun sequence.
ACCESSION   DASUTA010000169 DASUTA010000000
VERSION     DASUTA010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606849"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /gene="gpmA"
                     /locus_tag="VFZ80_03405"
     CDS             <1..693
                     /gene="gpmA"
                     /locus_tag="VFZ80_03405"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEX6286508.1"
                     /translation="ENLFTGWTDVGLTEVGETEARMAGRLMAAEGLEPDIMFTSVLKR
                     ATDTADLALEEAGWHDIPTKRSWRLNERHYGALQGLDKKETAEKFGAEQVLIWRRSYD
                     TRPPALEADDTRHPSHDELYADIDPEDLPATECLADVVDRMLPYWFDEIVPELQAGKT
                     VLIVAHGNSLRALVKHLDDVSEEDIVGLNIPTGIPLVYELDDDLRPIESHYLGDQEAA
                     QAAADAVSKQAG"
     gene            857..1096
                     /gene="secG"
                     /locus_tag="VFZ80_03410"
     CDS             857..1096
                     /gene="secG"
                     /locus_tag="VFZ80_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX6286509.1"
                     /translation="MSILTAIGIVIHIILSLALVALVLLHSGKGGGLSDVFGGGMSTS
                     NLTGSTVVERNLDRLTVVVGVLFGVSTFILTFALS"
     gene            1134..1220
                     /locus_tag="VFZ80_03415"
     tRNA            1134..1220
                     /locus_tag="VFZ80_03415"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1168..1170,aa:Leu,seq:aag)
     gene            1241..1876
                     /locus_tag="VFZ80_03420"
     CDS             1241..1876
                     /locus_tag="VFZ80_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6286510.1"
                     /translation="MSLVRIHNLSISLDGFATGEPQSADAPFGHAGERLHEWMFATRF
                     WDDSGTVGVDDAFADRHSPGIGAEIMGANKYGPPGWQDDPDWKGWWGPNPPFHTPTFV
                     LTHRPRPSIEMEGGTTFHFLDATPAEALEAARDAADGHDVRIGGGATVVRDFLSAGLI
                     DHMHLVQVPIVLGRGVRIWDGLEGLEDTFDIEAVSSPSGVTHLTLTRKIGS"
BASE COUNT          387 a          712 c          649 g          367 t
ORIGIN      
        1 gagaatctgt tcactggttg gaccgacgtc ggtctgaccg aagtggggga gaccgaggcg
       61 cgaatggcgg gccggctcat ggccgccgag ggcctcgagc ccgacatcat gttcacctcg
      121 gttctgaagc gggccaccga caccgccgac ctggccctgg aagaggcggg atggcacgac
      181 atccccacca agcggagctg gcgtctcaac gagcgccact acggcgcctt gcagggtctc
      241 gacaagaagg agacggcgga gaagttcggc gcggagcagg tgctgatttg gagacgatcc
      301 tacgacacca gaccacccgc cctggaggcc gatgacaccc ggcatccttc ccacgacgag
      361 ctctacgcgg acatcgaccc tgaagatctg cccgccacgg agtgtctcgc cgatgtcgtc
      421 gaccgcatgc tgccctattg gttcgacgag atcgttcccg agctgcaggc cgggaagacc
      481 gtgctgatcg tcgcccacgg caattcgctc cgggcgctgg tcaagcatct cgacgacgtc
      541 agcgaggagg acatcgtcgg gctgaacatc ccgaccggga tccccctggt ctacgagctc
      601 gacgacgacc tcagacccat cgagagccac tacctgggcg accaagaggc cgctcaggcc
      661 gccgccgacg cagtctccaa acaggccggc tgacgggttc cggctgaccg ttctcgcgca
      721 tcaaatgcgc tgaaaacgcg ccatttgatg cgcgaaaacg gttggtgggt gccccggcta
      781 gatcagagtg gggactgctg gtacaattcg cttcccgcct gatggcgggg tttccaccgt
      841 ctgagaccta tcgaacatga gcatcctgac cgcgatcgga atcgtcatcc acatcatcct
      901 ctccctggcc ctcgtggctc tggtgttgct gcactcgggg aagggcggtg gcctctccga
      961 cgtgttcgga ggcgggatgt cgacctcgaa cctgaccggc tcgacggtgg tcgagcgaaa
     1021 cctcgatcgt ctcactgtgg tggtcggggt ccttttcggg gtcagcactt tcatcctgac
     1081 cttcgccctg agttgataga gcgaccgata caatcgggct cgtagtcacc cctgtcggag
     1141 tggcggaatt ggaagacgcg ccaggctaag gacctggtgg ggtttacagc cccgtggagg
     1201 ttcgagtcct ctctccgaca cccaatcgag gagcacacga atgtcactcg tacggatcca
     1261 caacctgtcc atctccctcg acggcttcgc caccggagag ccgcagtcgg ccgatgcacc
     1321 cttcggccac gcgggcgaac gcctgcatga gtggatgttc gccacccggt tctgggacga
     1381 cagtggaacc gttggagtcg acgacgcgtt cgccgaccgt cacagtcctg gcatcggcgc
     1441 cgagatcatg ggcgccaaca agtatggccc gcccggctgg caggacgatc ccgactggaa
     1501 gggttggtgg ggccccaacc ccccgttcca cacaccgaca ttcgtcctca ctcaccgccc
     1561 ccgaccttcg atcgagatgg agggcggcac cacgttccac ttcctcgacg caaccccggc
     1621 cgaggcactc gaggctgccc gcgatgcggc cgacggtcac gacgtccgaa tcggcggtgg
     1681 cgccaccgtc gtccgcgact tcctgtccgc cgggctcatc gaccacatgc atctcgtcca
     1741 ggtcccgatc gtgctcggcc gcggtgtgcg catctgggac ggtcttgaag ggctcgagga
     1801 tacattcgac atcgaggcgg tctcctcgcc cagcggcgtc acccacctca cgctcacgcg
     1861 caagatcggc tcgtgaacgc cgacaccgaa ccgcaggccg cgcccagaca gccaagattg
     1921 acgaacaagc gttcgcggac ctgttcggtc aaggtcgccc gcgcgggagt tcgccaacct
     1981 ttcctgcatc tcgacagtcc accggcccgt cggcccacac agcgcacgac ttccgcccgc
     2041 cgctggtgca aactttcgca gacatgcatg ttatgacagt gtgtttagtc tggacgcgtt
     2101 tcgagccgac atggg
//
LOCUS       DASUTA010000170         2710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1476747, whole genome shotgun sequence.
ACCESSION   DASUTA010000170 DASUTA010000000
VERSION     DASUTA010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476747"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VFZ80_03425"
     CDS             <1..1083
                     /locus_tag="VFZ80_03425"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX6286511.1"
                     /translation="LVLDGFQQPTAVTAPTGDDRLFVAERFGAIRVIDENGVKLDSPF
                     LDITENVSSNGIERGLLGLAFHPDYANNGRFFAYYTDKDGRRQLSEFAVSPTDPNIAF
                     AASEKVIFEYEQPPNATDIRHYAGNVAFGPDGYLWVSMGDGADSRAQGQDPNTPYGTI
                     VRIDVDNGDPYAIPPDNPFVDGGGMADVWAYGLRNPWRFAIDPVDRLIYIDDVGLADQ
                     EEVNVVSIDAGGYNFGWSNMEGTRCFHEQDCDPADYTAPVLTYLHSAPDDDPDAVVGL
                     SITGGYVYRGSEIPEIAGTYFYSDWVSSWVRGFKFVDGQVTDDRDWTSDLGGEVGQIM
                     TYGLDGHGELYLATYEGGVYKFTAVR"
     gene            complement(1067..1402)
                     /locus_tag="VFZ80_03430"
     CDS             complement(1067..1402)
                     /locus_tag="VFZ80_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286512.1"
                     /translation="MWRPFSIVGLVGLLVATGVAFAAPAAETRLPVTMSQDERAASKT
                     VDEVVVVKGDHLWKISSRHLADHSPSTAVAPYWLQVIETNMPTLRSGDPDLIYPGEVV
                     RLPKLSERP"
     gene            1459..1686
                     /locus_tag="VFZ80_03435"
     CDS             1459..1686
                     /locus_tag="VFZ80_03435"
                     /inference="COORDINATES: protein motif:HMM:NF022749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 family protein"
                     /protein_id="HEX6286513.1"
                     /translation="MKVRIGIATTDKLLELEIDDAKAFKKEMERAVAEGGLGWFTDAK
                     GRTVGIPARSVAFIEMEDADGQRTVGFAPAG"
     gene            complement(1763..2038)
                     /locus_tag="VFZ80_03440"
     CDS             complement(1763..2038)
                     /locus_tag="VFZ80_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007147400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX6286514.1"
                     /translation="MTLTTITTDWRDLAACRDSEPTLFFPVGTTGPAVEQIEAAVAIC
                     NTCMVREDCLQYALETNQESGVWGGYAEDDRRRLRKRWLAERRRRSV"
     gene            2162..>2710
                     /locus_tag="VFZ80_03445"
     CDS             2162..>2710
                     /locus_tag="VFZ80_03445"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX6286515.1"
                     /translation="MIPREPPVAIVGHRGWPVRYPDNTLSGLMAACEVADLVEIDVRR
                     SGDGKLVLSHDPEIQGHVVSATTWSVLSEIDLGDMHHPALLDEALAALPDTPVQLEIK
                     NLPFEPGYEPDHRLALEAAERSRPGDIVTGFNPETILAVRRSFPDVLTGLAIAGGISL
                     DEVVALCLDVGHHALVPDHSLLT"
BASE COUNT          508 a          845 c          837 g          520 t
ORIGIN      
        1 ttggtcctgg acggattcca acagccaacc gccgtgaccg cccctaccgg tgacgatcgc
       61 ctcttcgtcg ccgagcgctt cggtgcgatc cgtgtcatcg acgagaacgg cgtgaagctg
      121 gactcaccat tcctggacat aaccgagaac gtctcctcaa acgggatcga gcgtggtctg
      181 ctcggtcttg ccttccatcc ggactacgcc aacaacggca ggtttttcgc ctactacacc
      241 gacaaggacg ggcgcaggca gttgtccgag tttgcggtct cgccgaccga cccgaacatc
      301 gcgttcgccg ccagcgagaa ggtcatcttc gaatacgagc agcccccaaa cgccaccgac
      361 atccgccatt acgccggcaa cgtggcgttc ggtcccgacg gttacctctg ggtctcgatg
      421 ggcgacggcg cagacagcag ggcgcagggc caggacccaa acaccccgta tggcaccatc
      481 gtccgcatcg acgtcgacaa cggtgacccc tatgcgattc ctcccgacaa cccattcgtc
      541 gacggtggcg gcatggccga cgtctgggct tacggtctgc gcaatccttg gaggttcgcc
      601 atcgacccgg tcgatcgtct catctacatc gacgacgtgg gcctcgccga ccaagaggag
      661 gtcaacgtgg tgtcgatcga cgcaggcgga tacaacttcg gttggtcgaa catggaagga
      721 acccgatgct tccacgaaca ggactgcgac ccggccgact acacggcacc ggtgctgacc
      781 tatctgcatt cggcgcccga cgatgatcca gacgccgttg tcggcctctc gatcactggt
      841 ggttacgtgt accggggcag cgagatcccc gagatcgccg gcacctactt ctactccgat
      901 tgggtgtcat cttgggtccg cggcttcaaa ttcgtcgatg ggcaggtgac cgacgaccgc
      961 gactggacca gcgatctggg aggagaggtg ggccagatca tgacgtatgg tctcgacggc
     1021 cacggtgagc tctatctcgc cacgtacgaa ggcggggtct acaagttcac ggccgttcgc
     1081 tgagcttggg taatcgcacc acctcacccg ggtagatgag atcaggatcg cccgatcgaa
     1141 gcgtcggcat gttggtctcg atcacctgga gccagtaggg ggcgacggcg gtgctggggg
     1201 agtgatctgc gagatgtcgg ctcgagatct tccaaaggtg atctcccttg accaccacga
     1261 cttcgtccac ggtcttggag gcggctctct cgtcctgaga catggtgaca gggagtctcg
     1321 tctctgcggc cggggcggcg aaggccaccc cagttgccac gagcagacca accagcccga
     1381 cgatggagaa cggtctccac atgtcttcga acgtacgccc gcagttcaac cattggaagg
     1441 gggttacgat cgatcgccat gaaggttcgc attggaatcg ccactaccga caagctcctc
     1501 gaattagaga tcgatgacgc caaggctttc aagaaagaga tggagagggc ggtggccgag
     1561 ggcggtctcg gctggttcac cgacgcgaag gggcgcactg tcggcatccc ggcgcgcagc
     1621 gttgcgttca tcgagatgga agacgccgac ggccagcgaa ctgttggctt cgccccggcg
     1681 ggctgatcac cgggttgtca tgaaatgaga agaggcgccg cctgtgagac aggttgcgcc
     1741 tctggatcct cttctggttc gttcagacgg agcgacgacg ccgctcggca agccagcgct
     1801 tgcggagccg acgacgatcg tcctcggcat agccacccca gacgccggac tcctgattgg
     1861 tctcgagggc gtactggagg caatcttcac gaaccatgca ggtattgcag atggccactg
     1921 cggcctcgat ctgctccacg gccggaccgg tcgtccccac agggaagaag agcgtcggct
     1981 ctgagtccct gcaggcggcg aggtcccgcc aatctgtagt gatggttgtg agggtcatgt
     2041 gttcctaact ttgtgatctt tttcacaagg gcatcgtcac gggggcggcc cggagatact
     2101 tgggggggtt ccggggcgaa cggtaacgtc cgaaacccac gttgtaaatg gatttcttcg
     2161 attgattccg agagaacccc ctgtcgccat cgttggccac cgtgggtggc cggtgcgtta
     2221 tcccgacaac acgctgagcg gtctcatggc tgcttgcgag gtcgccgatc tcgtcgagat
     2281 cgacgttcgg cgctccggtg acgggaagct ggtgctctct cacgaccctg agatccaagg
     2341 tcatgtcgtg tctgcaacaa cctggtcggt cctcagcgag atcgacctcg gcgacatgca
     2401 ccaccccgca cttctcgacg aggcccttgc cgccctgccg gacacaccgg tccagctcga
     2461 gatcaagaat ctcccgttcg agccggggta cgaaccggac catcgtctcg ccctcgaagc
     2521 ggccgagagg tcccggcccg gggacatcgt caccgggttc aatcccgaga ccattctggc
     2581 ggtgcgtcgg agcttccccg acgtgctcac ggggctggcc atagccggtg ggatatccct
     2641 cgatgaggtc gtcgctcttt gcttggacgt aggtcatcac gctttggttc cggaccattc
     2701 cctgctcacc
//
LOCUS       DASUTA010000171         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_828454, whole genome shotgun sequence.
ACCESSION   DASUTA010000171 DASUTA010000000
VERSION     DASUTA010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828454"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VFZ80_03450"
     CDS             <1..1073
                     /locus_tag="VFZ80_03450"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010078984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX6286516.1"
                     /translation="TANTMASVGEAIGLSLPGSASVPAVDHRLRDFARMSGEAVMALA
                     DAGITSRQILTRPAFENAITTVMALGGSTNAVLHLMAIAHEAGVDLELEDFDRISRKT
                     PHVGDLKPFGRFHMVDLDRVGGVPSVLRGLMDEGLLNGDVLTVTGKTMAENLANVSPP
                     EGEDVIRSFDDPLAHEGGIAVLRGNLAPEGAVVKLAGIELERREGPARVFEGEQAALA
                     ALFSHQIRPGDVVVIRTEGPKGGPGMREMLQITAAIKGAGLGKDVLLITDGRFSGGTT
                     GLCIGHIAPESEVGGPIGLVEEGDIIRVDVPERRLDVVVDDETLAERRANWTPHEVRY
                     STGALAKYAALVGSASSGAVTG"
     gene            1086..2084
                     /locus_tag="VFZ80_03455"
     CDS             1086..2084
                     /locus_tag="VFZ80_03455"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX6286517.1"
                     /translation="MTRPGRESERGLLGRMASEASDRVLDVVDPNIVLDHIDVDALIS
                     RVDINALLDRVDINDLLDRVDVNRLLERVDVDGLMDRVDVDALMARVDVPDLVERAGI
                     PDIVMESTSHLTGSALDLFRRPIVGLDAIVFRGLNRLIGRDLNEFPPGPGDLLQWVDD
                     HQAKKGGVTGRYAGPLTRLIAVMFDSVVVTFGFTLLVAGLVFVIRLFVPEFEIPESSG
                     IIYGIALTVWSFLYLWISYTVFGKTIGKMMLGVRVVSADGHVVLRSGQTFVRVITYPL
                     SFILFGIGLLGVVFNRKRQAWHDRFAKTAVVYDWGSRTATMPTPLADFLERRGAQV"
BASE COUNT          353 a          656 c          685 g          404 t
ORIGIN      
        1 tcacggcgaa cacgatggcc tcggtcggtg aggccatcgg tctctcactg ccgggttcag
       61 cctcggtgcc cgcggtcgat caccggctcc gtgacttcgc gaggatgtcg ggcgaggcgg
      121 tcatggcctt ggccgatgcc gggatcacgt cacggcagat cctgacccgg cccgcattcg
      181 agaatgcgat caccaccgtc atggccctcg gcgggtctac caacgccgtt ctccacctca
      241 tggccatcgc ccacgaggcc ggggtcgatc tcgagctcga ggacttcgac cggatatcac
      301 gtaagactcc tcacgtcggc gacctcaaac cgttcgggcg attccacatg gtcgacctgg
      361 acagggtcgg tggagtgccc agcgtgctca gggggctcat ggatgagggg ttactcaatg
      421 gtgacgtgct gacggtgacg gggaagacca tggccgagaa cctggccaat gtctctccac
      481 ccgaaggaga ggacgtcatc aggtctttcg atgacccact tgctcacgaa ggtgggatcg
      541 cggtgctccg tggcaatctg gcaccggaag gagccgtcgt gaaactcgcc ggaatcgagt
      601 tggagcgacg ggagggcccg gctcgggtgt tcgagggcga acaggccgct ctcgccgccc
      661 tgttctcgca tcagatccgc cccggcgatg tcgtcgtcat acgcaccgag gggccaaagg
      721 ggggccccgg catgagagag atgcttcaga tcaccgcggc gatcaagggt gccggccttg
      781 gcaaggacgt gctcttgatc acggatgggc ggttctccgg cggtaccact ggtctctgca
      841 tcgggcacat cgcccccgag tccgaggtcg ggggtccgat cggcctggtc gaggagggtg
      901 acatcatccg ggtcgatgtc ccagagcgca ggctcgacgt agttgtcgac gacgagaccc
      961 tggcggagcg gagggccaac tggacacccc acgaagtccg ctactccacg ggtgccctgg
     1021 ccaagtatgc cgcgttggtt ggatctgcct cttctggcgc ggtcaccggg tagcttctcc
     1081 ggcatatgac acgtccagga cgagaatcag agcggggcct cctgggcagg atggcctcgg
     1141 aggccagcga tcgagtcctc gacgtcgtcg atcccaacat cgttctcgac cacatcgacg
     1201 tcgatgccct catctcccgc gtcgatatca acgcgctgct ggaccgagtc gacatcaacg
     1261 acctcctcga ccgggtcgac gtcaatcgac tgttggagcg ggtcgatgtg gacggtctca
     1321 tggaccgcgt cgatgtggac gccctcatgg cgcgggtgga cgtacccgac ttggtcgaaa
     1381 gggctggcat ccctgacatc gtgatggaga gcaccagtca tctcaccgga tcggcgctcg
     1441 acctgttccg tcggcccatc gtgggtctcg acgcgatcgt cttccgtggc ctcaaccgcc
     1501 tgatcgggcg tgacctcaac gagtttccgc ccggccctgg tgacctgctc cagtgggtcg
     1561 acgatcatca ggcgaagaag gggggcgtga ccggtcggta tgcgggcccg ctcaccaggc
     1621 tcatcgcggt gatgttcgac agcgtggtgg tgacgttcgg gttcactctg ctcgtggccg
     1681 gcctcgtgtt cgtgatacgt ctcttcgtgc ccgagttcga gatcccggag tcgtcaggga
     1741 tcatctacgg catcgcactg actgtgtggt cgttcctcta cctctggatc agctacacgg
     1801 tattcggcaa gacaatcggc aagatgatgc tgggagttcg agtggtcagc gcagatggcc
     1861 acgtggtcct gcggtccggt cagacgtttg tccgggtcat cacgtacccg ctcagcttca
     1921 tcctcttcgg gatcggcctc ctcggtgtgg tcttcaacag aaaacggcag gcttggcacg
     1981 accgcttcgc caagacggct gtggtgtacg actgggggtc gcgcacggcc accatgccca
     2041 caccgctcgc cgacttcctg gaacgtcggg gcgcccaggt ctagccgccg tttcaacg
//
LOCUS       DASUTA010000172         4387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_181774, whole genome shotgun sequence.
ACCESSION   DASUTA010000172 DASUTA010000000
VERSION     DASUTA010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181774"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VFZ80_03460"
     CDS             <1..407
                     /locus_tag="VFZ80_03460"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX6286518.1"
                     /translation="LMDELGFFEKPEYRLGLLASQLFRSTRVVVDIGCHLGLTIPDSA
                     PLHGGEVWSYETAVDYIEHVGLQPHDVAVSEVLRYLGWPGQAITYKVGEREILAMRDR
                     AKARRDFSLKDFHRRILAIGPIRLDLLAEEMA"
     gene            404..1006
                     /locus_tag="VFZ80_03465"
     CDS             404..1006
                     /locus_tag="VFZ80_03465"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006054929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX6286519.1"
                     /translation="MTKLVFELDDAESRDRYKLLTGLVVPRPIGWIGTRRTDGTNNLA
                     PFSFFNVVSTHPPVVLFSAGSHRDRRKDSATLAEERGEFTVNIVSEDVVHAMSVTSES
                     FTADDDEFAIAGLTAIRGVRVDAPMVAESPANLECTVRQVMELGERTKLILGDVVAIH
                     VEENVLDGTRVDNDVLKAVGRMAGATYISTRERFDIDRPD"
     gene            complement(1011..2150)
                     /locus_tag="VFZ80_03470"
     CDS             complement(1011..2150)
                     /locus_tag="VFZ80_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX6286520.1"
                     /translation="MPRFRRSRALLVGLALVLTVALFSVDYTVQHGDTLGGIAREHDV
                     SLSDLIQANNISNPNLIYPGQKLTIPGSEEVYTVVFGDTLGRIAKRYKTSVSQLTSAN
                     NIPNPNIILVGQQILIPTGSSGSGSGPTGGSSNEGTISDRTGKVHVVKRGETLARIAA
                     QYRGVTADDIAAANGILNGIIYAGQGLYLSGPGYVASGAAGEAKYRVERGDRLGDIAH
                     SHGVAVNSLVSLNKISNPNLIRSGQVLRIPTGSEWVCPVEGASFMNDWGFPRGGGVRY
                     HEGNDLFVSRGTPVRAPVSGTVKQKTGTIGGLQFTLDGSDGVRYIGTHMDQFGKKGKV
                     KAGEIIGYVGNTGNAVGTSPHLHFGMYYQGTPVNPYPSLIAHGCD"
     gene            complement(2199..3239)
                     /gene="rlmN"
                     /locus_tag="VFZ80_03475"
     CDS             complement(2199..3239)
                     /gene="rlmN"
                     /locus_tag="VFZ80_03475"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX6286521.1"
                     /translation="MPYLHAPEELGELLTDEPGYRVDQLRDWLYRTPVLHAMDMTNLP
                     THVRDSIEESLWPFDIEMEQSADEDTTRKWLFRARDGAAIESVLMGYPKRATLCISSQ
                     AGCALGCTFCATGQFGFERHLEPGEIVAQVAYAQAFLRQIGMPGVPDHLTNIVFMGMG
                     EPLANYGRVREAIRRMIEVMGISARSITVSTVGIVPGIRRLADEPWPVYLAVSLHAAD
                     DELRSSLVPMNKRYPIAELEKAAWEYFERKGRRLSIEWTMMDGVNDTIDQADKLAAIA
                     NRLQAHVNLIALNPTPLSDARASTRLHIEEFAEALEKRGVNVTIRDTRGREIDAACGQ
                     LRVLSGISRPGR"
     gene            complement(3247..3558)
                     /locus_tag="VFZ80_03480"
     CDS             complement(3247..3558)
                     /locus_tag="VFZ80_03480"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX6286522.1"
                     /translation="MKRVAKVVGFIGGALAVIWAMRDRFISVATSREPQPPAFRSATP
                     SPPVTDIDGIGPVFAQRLTNAGIADIATLASASPDQVAEAASVSAARARSWIELANEG
                     G"
     gene            3604..>4387
                     /locus_tag="VFZ80_03485"
     CDS             3604..>4387
                     /locus_tag="VFZ80_03485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6286523.1"
                     /translation="MKVDFYYPPAPPEHAVEAAGEAAALGYDGFFTAETQFDPFLPIT
                     HAAAAEPGLEFGTAIAVAFPRSPTVMAMTAWDLARQTKGRFMLGLGTQVRGHIVRRFS
                     SLWAGKPAPQLREYIEAMRAVWDSWQNGSPLTYDGEFYQLSLMTPFFNPGPIPHPDVP
                     VYIAGVGPYLSALAGEMCNGFHVHPFHTVPYLDEIVLPNMEKAAKGAGRSLDDVEMVT
                     TVFIMTGNTEEEIEQAKEPVRQQISFYASTPSYRSVLEASGWD"
BASE COUNT          769 a         1339 c         1423 g          856 t
ORIGIN      
        1 ggctcatgga tgagctcgga ttcttcgaga aacccgagta tcggcttggc ctgctggcca
       61 gccagctgtt ccgctcgact cgagtggtgg tcgatatcgg ctgtcacctc ggattgacca
      121 tccctgacag cgccccgctt catggcggcg aggtctggag ttacgagact gccgtcgact
      181 acatcgagca tgtcgggctc cagccccacg acgtggcggt gtcggaggtg ctgcgatacc
      241 tcggttggcc agggcaggcc atcacctaca aggtgggtga acgggagatc ctggcgatga
      301 gagatcgggc gaaggcgagg cgcgacttca gtctcaagga tttccatcga cgaatcctgg
      361 cgatcggccc gatccgcctc gatctcctcg ccgaggagat ggcttgacga agctcgtatt
      421 cgagctcgac gatgcagagt cgcgcgatcg ctacaagctg ctgaccgggc tcgtagtgcc
      481 ccgcccgatt ggctggatcg ggacacgccg aaccgacggg accaacaacc tggctccgtt
      541 ctcgttcttc aatgtcgtgt cgacccatcc accggtggtg ctcttctcgg ccggtagcca
      601 tcgtgaccgc cgcaaggact cggccaccct ggctgaggag cgcggggagt tcacggtcaa
      661 catcgtctcc gaagacgtcg tgcacgccat gtcggtcacc tccgagtcgt tcacggcgga
      721 tgacgacgag ttcgccatcg ccgggcttac tgccattcgc ggcgtcagag tggatgctcc
      781 catggtcgcc gagtcgccgg ccaatctgga gtgcaccgtt cgccaggtca tggagctggg
      841 ggagcggacg aagctgatcc tgggtgatgt cgtcgccatc catgtggagg agaacgttct
      901 cgatggaacc cgggtcgaca acgacgtatt gaaggcggtc ggcaggatgg ccggagccac
      961 ctacatcagc acccgggagc gcttcgacat cgaccggccc gattgaagga tcagtcgcaa
     1021 ccgtgtgcga tcagtgaagg gtatgggttg accggcgtcc cctggtaata catgccgaag
     1081 tggaggtgag gcgaggtacc gactgcgttg cctgtgttgc cgacatatcc gatgatctct
     1141 ccggccttga ccttcccctt cttgccgaat tggtccatgt gagtgccgat gtagcgcact
     1201 ccgtctgatc cgtcgagcgt gaactggagc ccgccgatgg tccccgtctt ctgtttgact
     1261 gtccccgaca cgggtgcacg caccggggtg ccccggctga cgaagaggtc gttgccttcg
     1321 tggtatcgga caccccctcc ccggggaaag ccccagtcgt tcatgaacga ggctccctcg
     1381 accgggcaga cccactcgct gccggtgggg atccggagga cctggccgga gcgaatgagg
     1441 ttggggttgg agatcttgtt cagtgacacc agggagttga cggccactcc gtggctgtgg
     1501 gcgatgtctc cgagtcgatc gcctcgctcc actcgatact tggcttctcc ggctgcgccc
     1561 gaagccacgt atccggggcc gctgaggtac aggccctggc cggcatagat gatcccgttg
     1621 aggatgccgt tggcagcggc gatgtcgtcg gcagtgaccc cccgatactg ggcggcgatc
     1681 cgtgccagtg tctccccacg cttcacgacg tgcaccttcc cggtccggtc agagatggtg
     1741 ccctcgttgg acgaaccgcc ggtcgggccc gatcccgacc ccgagctccc cgtggggatg
     1801 aggatctgct ggccgacgag gatgatgttc gggttgggga tgttgttggc cgaggtgagc
     1861 tgggagaccg aagtcttgta cctcttggcg attcggccca gggtgtctcc aaagacgacc
     1921 gtgtacacct cttccgaccc cgggatagtg agcttctgac ctgggtagat gaggttcggg
     1981 ttggagatgt tgttcgcctg gatcagatcg gacagggaca catcgtgttc gcgggcgata
     2041 cctcccagcg tgtctccatg ctggacggtg tagtcgaccg agaagagggc gacggtgagc
     2101 accagcgcaa gaccgaccaa gagggcacgg gaacgtcgaa atctgggcat gtgaaggcac
     2161 aactccttca gcccctgact cgctccgcga gagtgagact acctacctgg tcgcgaaatt
     2221 ccagacaaaa cccgaagctg gccgcatgcg gcgtcgatct cgcggcctct ggtgtcccgg
     2281 atggtcacgt tcactcctcg cttctccaac gcctcggcga actcctcgat gtgcagtcgc
     2341 gtcgaggcgc gagcatcgct gaggggcgtg gggttgaggg cgatcagatt gacgtgggcc
     2401 tggaggcgat tagcgatcgc cgccagcttg tcggcctggt cgatggtgtc gttgactccg
     2461 tccatcatcg tccattcgat cgagaggcgc ctccccttcc tctcgaagta ctcccacgcc
     2521 gccttctcca gctctgcgat cgggtagcgc ttgttcatgg ggacgagtga gcttcgcagt
     2581 tcgtcgtctg ctgcgtgcag gctcacggcg agatagaccg gccagggctc gtcggcaaga
     2641 cgccgaatgc cgggcacgat ccccacggtc gacactgtta tggagcgggc ggagattccc
     2701 atcacttcga tcatgcggcg gatcgcctca cgaacccggc cgtagttggc caatggctca
     2761 cccatgccca tgaagacgat gttggtgaga tggtcgggca ctcccggcat cccaatctga
     2821 cgtagaaacg cctgggcgta ggcaacctgt gccacgatct ctccgggctc gagatgtcgc
     2881 tcgaagccga actgcccggt cgcgcagaag gtgcagccga gcgcacagcc ggcctgactc
     2941 gatatgcaca gagttgctcg cttggggtat cccatcagaa ccgactcgat ggcggcgccg
     3001 tcgcgcgctc tgaacagcca ctttcgagtg gtgtcttcgt ccgcactctg ttccatctcg
     3061 atgtcgaacg gccagagcga ttcttcgatc gaatctcgca cgtgggtggg cagattggtc
     3121 atgtccatcg cgtggagcac cggagtccgg tagagccagt cacggagctg gtcgacccga
     3181 tagccgggtt catccgtgag aagctccccg agctcctcgg gggcatggag gtagggcacc
     3241 gggcgcctaa ccgccttcgt tggcgagctc gatccaggag cgagctcgtg cggcactgac
     3301 gctcgctgcc tcggcgacct gatcggggct ggcactcgcc agtgtggcga tgtcggcgat
     3361 gcccgcgttg gtgagccgtt gggcgaagac cgggcctatt ccatcgatgt cggtaacggg
     3421 tgggctggga gtcgccgagc ggaaagcagg tggctgtggt tcccgggagg tggctaccga
     3481 gatgaagcga tcgcgcatcg cccatatcac ggcgagggcg cctccaatga agccgacgac
     3541 tttggcaact cgtttcatcc ttctccaagg ctacaggttc gggcgtttct ctacattgac
     3601 ggcatgaaag tcgacttcta ttacccgcca gcaccccccg agcacgccgt tgaggctgcg
     3661 ggagaggccg ccgcgctggg ctacgacggg ttcttcaccg ccgagaccca gttcgacccg
     3721 tttctcccca tcacccacgc cgcggcagcg gagcccggtc tcgagttcgg gacggcgata
     3781 gccgtggcgt ttcccagatc cccaaccgtc atggctatga ccgcctggga ccttgcgcgc
     3841 cagaccaagg gcaggttcat gctcggcctg gggacgcagg tgcgtggcca tatcgtcaga
     3901 cggttcagct cgttgtgggc cggcaagccc gctccacagc tgcgcgagta catcgaggcg
     3961 atgcgcgcgg tgtgggacag ctggcagaac gggtctcctt tgacatacga cggcgagttc
     4021 taccaactgt ccctgatgac acctttcttc aacccgggac ccatccccca cccggacgtg
     4081 ccggtgtaca tcgccggagt gggcccgtat ctgagcgccc tagctggtga gatgtgcaac
     4141 ggcttccacg ttcatccttt ccacaccgtc ccgtacctcg atgagatcgt gcttcccaat
     4201 atggagaagg cagcgaaggg agccgggagg tcgcttgacg acgtcgagat ggtgacaact
     4261 gtcttcatca tgaccgggaa caccgaagag gagatcgagc aggccaagga gccggtgcgg
     4321 cagcagatct cgttctatgc gtcgacaccg tcatatagat cggtgttgga ggcgagcggg
     4381 tgggact
//
LOCUS       DASUTA010000173         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1477511, whole genome shotgun sequence.
ACCESSION   DASUTA010000173 DASUTA010000000
VERSION     DASUTA010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477511"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFZ80_03490"
     CDS             complement(<1..309)
                     /locus_tag="VFZ80_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286524.1"
                     /translation="MATTSSERRIGGSEDVSDVYERYGSSDFLASIMGMFAGLGVLVF
                     LSALIAAGAGGIDYQLNVINTEGTLDEASIVGLAVAAIVVFASFVAGGFAAGRMARYN
                     G"
     gene            complement(435..578)
                     /locus_tag="VFZ80_03495"
     CDS             complement(435..578)
                     /locus_tag="VFZ80_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286525.1"
                     /translation="MSIVLALALSLGACDDGDDDGDNGDTIDPGTDVTTTTLLDMTTT
                     TLP"
     gene            complement(707..871)
                     /locus_tag="VFZ80_03500"
     CDS             complement(707..871)
                     /locus_tag="VFZ80_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286526.1"
                     /translation="MPVHRGKDSDGPYYQWGDSGKKYHYEPGKKSSREKAKDKAERQG
                     KAARASGYKG"
     gene            998..>2098
                     /locus_tag="VFZ80_03505"
     CDS             998..>2098
                     /locus_tag="VFZ80_03505"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286527.1"
                     /translation="MRPSILDEELFDRLATDDGAHLASVFIPTHSSGRDVSQDRIRLK
                     NQLRRVDAELEGLGLKPRAREDRLRITSDLLEDREFWEHQAEGLAVYIHEDGNVTAVA
                     VNYPVNESAIVMPVFLVRPLLSELKGPELPVLALTRDEVGLFVANLRGARRSLVDLPG
                     SFEDVNWFVDREPQRQQHPDRTDTTRARHGHDPSSREDEDVNRFLREVDRALPADDPL
                     VVLGDDNLVSRFGTITDRHVLSPENSGLQSPFTESEVMERTAQTLRSLAEQRERDAIA
                     EANAEIGMGNATTEIAAATSAAITGRVRHLVLDPTMDPVWGRIEETTLEVVQHDDKEH
                     ADVDLIDRVVVLSMRNGAEVTPILRGSSDHPMV"
BASE COUNT          399 a          656 c          632 g          411 t
ORIGIN      
        1 gccgttgtat ctggccatcc gcccggctgc gaagccgccg gcgacgaacg aggcgaaaac
       61 gactatggcc gccaccgcga ggcccacgat cgaggcttcg tcgagggtgc cttcggtgtt
      121 gatcacgttc agctggtaat cgatgccccc tgctccggct gcgatcagag cggagaggaa
      181 gaccaggact ccgagcccgg cgaacatgcc catgatcgaa gccaggaaat cagagctccc
      241 gtagcgctcg tagacatcgg atacgtcctc cgaaccgccg atgcggcgct ccgaactggt
      301 agtggccatg gattcctcct gtctcgaata ccccttctca ataccccgac ggattgctct
      361 cccaaaccaa gcctgcatta gaacggccgg ctgcagactg cagccggccg ttcgcattcc
      421 cgttgggttg tcgcctacgg gagagtggtg gttgtcatat ccagcagcgt cgtggtcgtc
      481 acgtcggtcc caggatcgat cgtgtcgccg ttgtcaccgt catcgtcgcc gtcatcacaa
      541 gctcccaaag agagggcaag tgccagcacg atgctcatga acacggttga tttcttcctg
      601 tggcgcattc ggttcttctc cttgttcggc cggagcaagg cgcgtgccca gtccgcccga
      661 tatcgcgtga tcgggcccag taccccggag tgccggggac caaacctcag cccttgtagc
      721 ccgaggcccg ggccgccttt ccctggcgtt ccgctttgtc tttcgccttc tcacgtgacg
      781 actttttccc cggctcgtag tggtacttct tgccggaatc accccactgg tagtagggcc
      841 cgtcactgtc cttgcctcga tgaacaggca tgcttgcctc cttggctcca gcgactgcga
      901 gatacccggc gtggcttggg ggcaaacgag ccgtttcccc gtctctgtcg gcgggtaggg
      961 aggaaaatga gctcgcgcgc actacggagg ccaaacagtg agaccatcca tcctcgatga
     1021 ggagctcttc gatcgtctgg cgaccgacga cggtgcccac ctcgcatccg tgttcatccc
     1081 gacgcactcc tcgggtcgcg acgtgtccca ggatcggatt cggctcaaga accagctgag
     1141 gcgtgtcgat gccgagctgg aggggcttgg actcaaaccc cgcgcacgcg aggaccggct
     1201 tcgaatcaca tccgatctgt tggaagatcg tgagttctgg gagcatcagg ccgaaggtct
     1261 cgctgtatac atccacgagg acggcaatgt cactgcagtt gccgtgaact acccggtcaa
     1321 cgagtcggcg atagtcatgc ccgtgttcct cgtcaggccc cttctcagcg agttgaaggg
     1381 cccggagctg cccgttttgg cgttgaccag ggacgaagtg ggtctgttcg tggcgaacct
     1441 gaggggggct cgacgctctc tcgtcgatct tcctggctcc ttcgaagatg tgaactggtt
     1501 cgtagacagg gagccacagc gtcagcagca tcccgatcgg accgatacaa cacgtgcccg
     1561 ccacggccat gacccgtcct cccgtgaaga cgaggacgtc aatcgctttc tacgtgaggt
     1621 cgaccgagcg ctgcccgccg acgatccctt ggtcgtgctc ggtgacgaca acctggtctc
     1681 gaggttcggg acgatcaccg accgtcatgt gctgagtccg gagaacagtg ggctgcaatc
     1741 acccttcacg gagagcgagg tcatggagag gaccgcccaa accctgcgga gcctcgccga
     1801 gcaacgagaa cgagatgcta ttgccgaggc gaatgctgag attggaatgg gcaatgcaac
     1861 cacggagatc gccgcggcga cctccgccgc aatcacaggg agagtcagac atctcgtgct
     1921 cgatcccact atggaccctg tttggggacg catcgaggag acgacgctcg aggtcgttca
     1981 acacgacgac aaggaacacg ccgatgtcga tctcatcgac cgcgtggtgg tcctatctat
     2041 gcgcaatggg gcggaagtga ctcccatcct gagagggtcg agcgatcatc cgatggtc
//
LOCUS       DASUTA010000174         2711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1477954, whole genome shotgun sequence.
ACCESSION   DASUTA010000174 DASUTA010000000
VERSION     DASUTA010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477954"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFZ80_03510"
     CDS             complement(<1..219)
                     /locus_tag="VFZ80_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6286528.1"
                     /translation="MDEPAGVVPRTARAPSDAQRFRFARLWTAAVASNLGDGLVLAAF
                     PLVATAITVDPVAISGIAVASGLPWLILA"
     gene            293..1141
                     /locus_tag="VFZ80_03515"
     CDS             293..1141
                     /locus_tag="VFZ80_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX6286529.1"
                     /translation="MPEHSILLSTGQLSLGLSRCGPISPHRTEEEIESQFILVFPLAG
                     VFGVDWEGWGTSATPSKAIMFSMGQTRRVDHPHGGHDRSAFISMDPQFAEAFLNGSGE
                     FRVAAANTSPGLDYRLRRLIAAARIGCVSSLEVEEFTTGAMEELTRGDAVAPPDARRA
                     VISEAETYLSVHFRESCDLSTLARAVGYSPHHLSRLFKQVTGETVSRRRTRLRLASAI
                     SEILDGADDLSRVAVDSGFYDHSHMTVAFRSHLGIRPNEVRSSAKSDFSSSRQVSRAG
                     PIGSQR"
     gene            1283..2464
                     /locus_tag="VFZ80_03520"
     CDS             1283..2464
                     /locus_tag="VFZ80_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX6286530.1"
                     /translation="MANERPEMLDKSSTLYFGPWYRRSPFFDSTLAAGCTAYDIYNHM
                     YLPAYYDDPDAEYWALVNGVTMWDVGVERTVEVSGKDADRLVDFITCRDLSRCAVGQG
                     KYMPVIAPDGGIVNDPVLLHVEENKWWMQLADSDAGLYALGVAAGRFDVDVTLPDVHP
                     MQVQGPLAAKTLEKLIGPSVYDIKYYWLVHETIIGIPVVISRTGWTAVPGFEINLLDS
                     SMGAELWDAVAAAGGEFDIRPIAPCEARRIEAGIFNYGSDMTLDDTPLHVSGMERLVE
                     PQSQYYLGKDALERVAAEGVDRKLVGMFLEGDQLRAELSRVWPAYHDDREVGRVTDAV
                     WSPGLERNIGYVWVPIGLAEPGTTLDVVTEDGDELTATVASLPFVDPQKKVPAGSLVA
                     T"
BASE COUNT          480 a          844 c          878 g          509 t
ORIGIN      
        1 cgcgaggatc aaccaaggga gcccggaggc gaccgcgatc cccgagatcg caaccggatc
       61 gacggtgatc gccgtggcta cgagcgggaa tgctgcaagc accagcccgt caccgaggtt
      121 ggatgccacc gcggctgtcc agagtcgagc gaagcgaaat cgttgggcgt cggagggggc
      181 ccttgccgtt ctcgggacca cacctgctgg ctcgtccatg gcgagagcat cccaaggcgg
      241 accagtgcgc actaggagat tcgtgcgaac ttggaccaga tagagtcgag gggtgccgga
      301 gcactcgatc ctcctctcca ccggccagct gagcctaggg ctctctcgat gcgggccgat
      361 atccccgcac aggacggaag aggagatcga atctcagttc attctggtgt ttcccctggc
      421 cggcgtgttc ggcgtcgatt gggaggggtg gggcacgtcg gccacgccct cgaaggcgat
      481 catgttcagc atgggtcaga cccgccgggt cgatcatcca catggtgggc acgaccgcag
      541 cgccttcatc tcgatggacc cgcaattcgc cgaggccttc ttgaacggat cgggcgagtt
      601 ccgggtcgct gccgccaaca ccagccctgg tctcgattat cgcttgcggc gcctcatcgc
      661 tgcggcgagg atcggatgtg tcagctcgtt ggaggtcgag gagttcacga ccggtgccat
      721 ggaagaactg acgcggggcg acgcagtcgc gccccccgat gcgcgacggg ctgtcatctc
      781 cgaagctgag acttacctgt cggtgcactt tcgtgagagc tgcgatttgt ccacacttgc
      841 tcgggccgtc ggatactcac cccatcacct gagccgactg ttcaagcagg tgacaggtga
      901 gaccgtctca cgccggcgga cgcggctgcg cctcgccagc gcaatctcgg agatcctcga
      961 cggtgcggac gatctctcca gagtggctgt cgactccggg ttctacgacc actcacacat
     1021 gaccgtcgct tttcgatctc atctcggcat tagaccgaac gaggtgagat cgagtgccaa
     1081 atccgacttc agttccagtc gacaggtctc cagggcgggc ccgattggat cacagaggtg
     1141 acctccgtcg aggcgtagcc gctgcgtagg aacgtcagac ttgctccacg agagcgaggc
     1201 gacatctgac tcgtgttccg gcgggcgtcc ctggcctccg acgtctattg ttgaacggcc
     1261 gttcaatcaa cgaggagagt cagtggccaa cgaacgtccc gagatgctcg acaagagctc
     1321 gaccctctac ttcggcccgt ggtaccggag gtcgccgttt ttcgactcga ctctcgctgc
     1381 cggctgcacc gcctacgaca tctacaacca catgtacctg ccggcctatt acgacgaccc
     1441 cgacgccgaa tactgggcgc tggtcaacgg ggtgaccatg tgggatgtcg gagtggagcg
     1501 caccgtggag gtgtccggca aggatgcaga cagactcgtt gacttcatca cctgtcgcga
     1561 cctgagcaga tgcgccgtcg gccagggcaa gtacatgccg gtcatcgcac ccgacggcgg
     1621 catcgtcaac gatccggtgc tgctccacgt cgaagagaac aagtggtgga tgcagctcgc
     1681 cgactccgac gccggcctct atgccctcgg tgtcgccgcc ggtcgcttcg atgtagacgt
     1741 gacactcccg gacgtgcatc cgatgcaggt gcaggggccg ctggcggcca agacgctgga
     1801 gaagctgatc gggccttcgg tctatgacat caagtactac tggctggtcc acgagacgat
     1861 cattggcata cccgtggtca tcagccgcac cggttggacg gcagtgcccg gattcgagat
     1921 caacctgctc gactcgtcga tgggagccga gctgtgggac gcggtggccg ccgcgggcgg
     1981 tgagttcgac atccggccga tcgcgccgtg cgaggctcgc cgtatcgagg cgggcatctt
     2041 caactacgga tcggacatga ccctcgacga cacaccactc cacgtcagcg gcatggagcg
     2101 actggtggag ccgcaatccc agtactacct gggcaaggat gccctggagc gggtggccgc
     2161 cgaaggcgtc gatcgcaagc tcgtcggcat gttcctcgag ggtgatcagt tgcgagccga
     2221 gctgtcacgc gtgtggccgg cctatcacga tgaccgtgag gtggggaggg tcaccgacgc
     2281 ggtgtggtct cctggtctgg agcgcaacat cggctatgtg tgggtcccga tcgggttggc
     2341 cgagccgggc accactctcg acgtcgtcac cgaggacggg gacgagctga ctgccactgt
     2401 cgcctctctg ccctttgtcg atccgcagaa gaaggtcccg gcagggagtc tcgtcgccac
     2461 ctagtccccc acattcgaaa tggtctttgg cctctggtca gggaggtcga ctcgttcgaa
     2521 tatgggatgg caaagacgat ttccggtgcg ccggtcgact cgagacccga cgccccgggt
     2581 ctggaggtgt tctcatgatg cgacgcccgg tggacaccgg ctcttctcac aagcgtgcct
     2641 cgagggttcc gatcgccggc ctcatctggg ttggcttctt ggtggccgcg tttctcctcc
     2701 tggtggacgt c
//
LOCUS       DASUTA010000175         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_829853, whole genome shotgun sequence.
ACCESSION   DASUTA010000175 DASUTA010000000
VERSION     DASUTA010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829853"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..961
                     /gene="pip"
                     /locus_tag="VFZ80_03525"
     CDS             8..961
                     /gene="pip"
                     /locus_tag="VFZ80_03525"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEX6286531.1"
                     /translation="MVGLRRRDIPPNQTGSLDVGDGYSVYWETWGDPSGVPVVFVHGG
                     PGGEYVPGHREMFDPGRYNVVFFDQRGCGRSRPLASDPDADLSANTTHHLIRDMESIR
                     EHVGIEKWAVHGLSWGTTLGLAYAEAHPGRVIGVMLGLVGLTTKREVKWITEGVSRIF
                     APQWERFSSFVPEELADLPLVDAYARMLFDPDPEKCAAAADEWCIWEDTHMGLAPGAG
                     PRLQNEDNAYKLQFARLVTHYWSNGAFLGETQLLDSAPTLNGIPGVLVHGRYDVSSPI
                     ETPWLLSKKWESAELVVVDDAGHGGGSLADAFAAGLEKVTS"
     gene            989..2071
                     /locus_tag="VFZ80_03530"
     CDS             989..2071
                     /locus_tag="VFZ80_03530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX6286532.1"
                     /translation="MAYDLGNEELNERLRSLVADSSENENQDLIEELIVTALKLHRDG
                     ATRGDLKMINTAVKEMRYSNLVFSRHDEPKVTIYGSARTEPDDPNYKLTEEFAATMAS
                     HGWGIITGAGPGIMEAGSKGAGIENSYGVNIRLPFEASANEYIAEGRTVNFKYFFTRK
                     LGFVKESNAFVIMPGGFGTLDEAFELITLIQTGKSDLHPIVLLEAEGTGYWEPLMAFT
                     TDILLGKGMISESDLALFLQTTDPQEASHHILEFYTNYHSQRYTAGKLILRLRQAPTD
                     AELARLNEDFSDIIVEGVIERAEPNQAEVEDQDALDMDRITFYFDRRHFGRLRQLIDR
                     LNDLASLPETTEIPHPISEEQAERPW"
BASE COUNT          422 a          657 c          653 g          381 t
ORIGIN      
        1 tgagggcttg gtcggattga gacggaggga catcccaccc aaccagactg gttccctcga
       61 cgtaggtgac ggctactcgg tctactggga gacgtggggc gatccctcgg gtgtgcccgt
      121 ggtcttcgtg cacggaggac ccggtgggga gtatgtgccc ggacaccgcg agatgttcga
      181 ccctggtcgt tataacgtcg tcttcttcga ccagcgaggg tgcggccgaa gccggcccct
      241 cgccagtgac cccgacgccg acctgagcgc caacaccacg catcatctca ttcgtgacat
      301 ggagtcgatc cgggagcacg ttgggatcga gaagtgggcg gttcatgggc tttcctgggg
      361 gacgaccctg gggctcgcct acgccgaagc acacccgggc cgggtcatcg gtgtgatgct
      421 gggtctggtt ggcctgacga cgaaacggga ggtgaaatgg atcaccgaag gcgtgtcacg
      481 gatcttcgcc ccgcagtggg agagattctc atcctttgtc cccgaggaat tggccgatct
      541 cccgcttgtg gacgcttacg cccggatgtt gttcgacccc gatcccgaga agtgtgcggc
      601 agccgccgac gagtggtgca tctgggaaga cacccacatg gggctggctc ccggcgccgg
      661 cccccggctg cagaatgagg acaatgccta caagctgcag ttcgccaggc tcgtcaccca
      721 ctattggagc aacggagctt tcctcggcga gacccagttg ctcgacagcg ctccgacgct
      781 gaatggcatc ccgggtgtcc tggtccacgg tcgctacgac gtcagctcgc cgatcgagac
      841 gccatggctc ctctcgaaga agtgggagtc ggccgagctg gtggtggtcg acgatgcggg
      901 tcacggaggc gggtctctcg ccgatgcctt cgccgccggc ctggagaagg tgacgtcgtg
      961 agcattggcc cggtacgttg gacgcgcaat ggcttatgac ctcgggaacg aggaactgaa
     1021 cgaacgactc cgcagcctcg tcgcagacag ctccgaaaac gagaaccaag atctcatcga
     1081 agagctgatc gtcaccgccc tcaagctgca tcgcgatggg gccacccggg gcgacttgaa
     1141 gatgatcaac accgctgtca aggagatgcg ctactcgaac ctcgtgttca gccgtcacga
     1201 cgagcccaag gtcaccatct atggctcggc tcggacagag cccgatgatc ccaactacaa
     1261 gctgaccgag gagttcgccg ccacgatggc gagccatgga tgggggatca tcaccggggc
     1321 ggggccgggg atcatggagg cgggaagcaa aggcgccggc atcgagaact cgtacggtgt
     1381 caacatccgg ctcccgttcg aggcctctgc caacgagtac atcgcagaag gccggaccgt
     1441 gaacttcaag tacttcttca cccggaagct ggggttcgtc aaggagtcca acgccttcgt
     1501 catcatgccg ggagggttcg ggactctcga cgaggccttc gagctgatca ccctgattca
     1561 gaccgggaag tcagaccttc acccgatcgt cctgctcgaa gcggagggca cgggctattg
     1621 ggagcccctg atggcgttca cgaccgacat tctccttggg aaagggatga tctctgagtc
     1681 cgatctcgcg ctgttcctcc agaccacaga cccccaggag gcctctcacc acattctcga
     1741 gttctacacc aactaccact cccagcgcta caccgccggc aagttgatac tgagattgcg
     1801 ccaggctccc accgatgccg agctggcccg actcaacgag gacttctcgg acatcatcgt
     1861 cgaaggtgtg atcgaacggg ccgagcccaa ccaggccgag gtcgaagacc aggatgcgtt
     1921 ggacatggac cgaatcacct tctatttcga ccgccgccac ttcgggcgcc tacgtcagct
     1981 catcgatcgg ctcaatgacc tggccagcct tcccgaaacc acggagatcc cccatccgat
     2041 cagcgaggag caggcggaaa gaccctggta aacggcttcc cgccttggtg atttccacag
     2101 attgcctatt aga
//
LOCUS       DASUTA010000176         3321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_829901, whole genome shotgun sequence.
ACCESSION   DASUTA010000176 DASUTA010000000
VERSION     DASUTA010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829901"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VFZ80_03535"
     CDS             complement(<1..300)
                     /locus_tag="VFZ80_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002858071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6286533.1"
                     /translation="MTATTDRRPPRILIVEDEPGLADSVRYALETEGFEVDVADSGVK
                     GLDLARRGNPDLVLLDLMLPEMSGLDVCRLIRSSSDVPIIMLTAKDSEADKVTGLE"
     gene            complement(284..991)
                     /gene="phoU"
                     /locus_tag="VFZ80_03540"
     CDS             complement(284..991)
                     /gene="phoU"
                     /locus_tag="VFZ80_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX6286534.1"
                     /translation="MTDTRDTNVHFHAELDGLERKVLSMVDRAEQMIEMAVEAVTKDD
                     LELAGRVIALDDDIDQTYFEVHQGWTTLMARHQPMGSDLRKMSILLQLNLTFERMGDQ
                     CVNIAKIAQFNSGLPRVDRICEQVREMGDLVRPMVRTAIEAYVRGDVDEARLLPAMDH
                     PVDRLNANMYKETVAVRDNPQLLEWATKMMMVSRALERIGDQAVDFAEQTAYLITGER
                     AEFDEEGLARALDDGDD"
     gene            complement(988..1764)
                     /gene="pstB"
                     /locus_tag="VFZ80_03545"
     CDS             complement(988..1764)
                     /gene="pstB"
                     /locus_tag="VFZ80_03545"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX6286535.1"
                     /translation="MRTEGMDVYYGDFKAVRDVTMGFGKNEITALIGPSGCGKSTVLR
                     CLNRMNDLIPTARVDGKVYFHDKNIYDADVDPVEVRRRIGMVFQKPNPFSKSIYDNVA
                     WGAKINGFKGKKSDMDDLVQRALEQAALWEEVKDKLEESGYSLSGGQQQRLCIARAVA
                     PNPEVLLMDEPCSALDPIATLRIEELMLDLKEKYTIIIVTHNMQQAARVSDRCAFFTT
                     EVNEKSDTRTGVLVEYDATDKLFTAPGDQRTDDYITGRFG"
     gene            complement(1896..>3321)
                     /gene="pstA"
                     /locus_tag="VFZ80_03550"
     CDS             complement(1896..>3321)
                     /gene="pstA"
                     /locus_tag="VFZ80_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX6286536.1"
                     /translation="PTGVRMSVETPERERTDHYPSDDEYLGLLSRRKRRATWFQLACL
                     GSLVIAVVALVTLLYTIINDSFGLVAVVNQNNPEDVVADLGHDPETVTLADLSYDDLV
                     DLVAANVSSGVGRRMEREQRFYEDRLVFESDQVWIEICASEEAPTGCALGPRSQPNVL
                     QLVNERVVVPDVIATEDMVPSLLEPEVFQAEVLNAFDTSPERFGDYTADQVSFEWRAW
                     FNPTFLVTPQNSVPEIAGIRTAILGSAWLVLITVAFAVPVGVGAAIYLVEFARPSRFN
                     DLIQTNINNLAGVPSIIYGMLGLAILVRGLEAFTSGSIFTDGVPPSDNGRTIISAGLT
                     LGLLTLPVVIISAQEALKAVPDSLRQAGLSLGATKWQTVRSQVLPVAMPGILTGVILA
                     VARAIGETAPLILVGAASFITFDPTGPFSKFTALPIQIFQWTGRPEEEFQRIAAAAII
                     ALLILLLTLNAVAVILRNRYSRRV"
BASE COUNT          595 a         1028 c         1055 g          643 t
ORIGIN      
        1 ctcgagaccg gtgaccttgt ccgcctcgga gtccttggcg gtgagcatga tgatggggac
       61 atccgaggac gaccggatga gacggcacac gtcgagaccc gacatctcag ggagcatcag
      121 gtcgagcagg accagatcgg gattccccct acgtgccagg tcgaggcctt tgacaccaga
      181 atcggcgacg tccacctcga agccctcggt ctccagggcg taacggaccg agtcggcgag
      241 ccccggctcg tcctcgacga tcaggatgcg gggtggccgg cggtcagtcg tcgccgtcat
      301 cgagggctct cgccaggcct tcttcgtcga actcggccct ctcacccgtg atcaggtagg
      361 cggtctgctc ggcgaagtcc acggcctggt ccccgatgcg ctccaacgcc cgggagacca
      421 tcatcatctt cgtggcccat tccaggagct gcgggttgtc cctgacggcg actgtctctt
      481 tgtacatgtt ggcgttgagc ctgtcgacgg gatgatccat cgccggcaac agtcgcgcct
      541 catcgacatc ccccctcaca tacgcctcta tcgccgtgcg aaccatcggg cgcaccaggt
      601 ctcccatctc ccgaacctgc tcgcagatcc gatcgacacg tggcagcccg ctgttgaact
      661 gggcgatctt ggcgatgttc acacactgat cccccatccg ctcgaacgtg aggttgagct
      721 gaaggaggat ggacatcttc ctcaggtcgg atcccatcgg ttggtgacgg gccatcagcg
      781 tcgtccatcc ctgatggacc tcgaagtacg tctggtcgat gtcgtcgtcg agcgctatca
      841 ccctgccggc caactcgagg tcatccttgg tcaccgcttc gacggccatc tcgatcatct
      901 gctcggcacg gtcgaccatg gacagcacct tgcgctccag cccgtcgagc tcggcgtgga
      961 aatggacgtt ggtgtccctg gtgtcggtca tccgaacctg ccggtgatgt agtcgtccgt
     1021 gcgctggtcg cccggagcgg tgaacagctt gtcggtggcg tcgtactcga cgagcacgcc
     1081 ggtgcgggtg tcgctcttct cgttgacctc ggtggtgaag aaggcgcagc ggtcgctgac
     1141 ccgggcggcc tgctgcatgt tgtgggtcac gatgatgatc gtgtatttct ccttcaggtc
     1201 cagcatgagc tcttcgatcc gtaacgtggc gatggggtcc agagcagagc agggctcatc
     1261 catgagaagt acttccgggt tgggagcgac ggcgcgcgcg atgcagagac gttgctgctg
     1321 tccaccagac agtgaatagc cggactcctc gagcttgtct tttacctcct cccagagcgc
     1381 agcctgctcg agggcccgtt gcaccagatc gtccatgtcg gacttcttgc ccttgaaccc
     1441 attgatcttg gcgccccagg cgacgttgtc gtagatcgac ttcgagaacg ggttgggctt
     1501 ctggaacacc atgccgatgc gccgccgaac ctccaccggg tcgacgtcgg cgtcgtagat
     1561 gttcttgtcg tggaagtaga ccttgccgtc gacccgagcg gtggggatca ggtcgttcat
     1621 gcggttgaga cagcggagga cggtggactt gccacacccg gagggaccga tgagagcggt
     1681 gatctcgttc ttgccgaaac ccatcgtgac gtcgcgcacg gccttgaaat cgccgtagta
     1741 gacgtccata ccctcggtcc gcatggcggg gacggcggtg ccgaggcctg cggtcttctc
     1801 ggactctcca aggccggcct cggaggtgac ctcgggggcg tcgtcgggtt cgatcatgtg
     1861 ggtcacctgc tcggcagtct gctcctgagt cacggtcaca ctctcctgga gtatctgttg
     1921 cgaagtatca ccgccaccgc attcagggtc aacaagagga tgagcaaggc gatgatcgcc
     1981 gccgcggcga tccgctggaa ctcctcttca ggccttccgg tccattggaa gatctggatc
     2041 ggaagggcgg tgaacttgga gaagggcccg gttgggtcga aggttatgaa gctggctgct
     2101 ccgaccagga tcaggggcgc cgtctcaccg atcgccctgg ccacagccag gatgacgccg
     2161 gtgaggatgc ccggcatggc cacggggagc acctgacttc gaacggtctg ccacttggtg
     2221 gcgcccaggc tcagcccggc ctggcgcaag gagtcgggca ccgccttgag cgcctcctgg
     2281 gcactgatga tcaccaccgg aagggtcagc agacccaggg tcaagccggc ggagatgatg
     2341 gtgcggccat tgtccgacgg cggcacgccg tctgtgaata tcgacccgga ggtgaacgcc
     2401 tccagacctc gaacgagaat ggccagcccg agcatcccgt agatgatcga gggcacgccg
     2461 gcgaggttgt tgatgttggt ctggatcaag tcgttgaatc gtgacggccg tgcgaactcg
     2521 acgagataga tggccgctcc gactccgacc gggaccgcaa acgccaccgt gatcagcacc
     2581 agccatgccg acccgaggat tgcggtgcga attccggcga tctcgggcac cgagttctgt
     2641 ggggtcacga ggaaggtcgg gttgaaccac gcccgccact cgaagctgac ctggtccgcg
     2701 gtgtagtcac cgaagcgctc gggcgaggta tcgaaggcat tgagcacctc tgcctggaac
     2761 acctccggtt cgaggaggga gggaaccatg tcctcggtgg caatgacatc ggggaccacg
     2821 acccgctcgt tgaccaactg cagcacgttg ggttgactgc gtgggcccaa cgcgcagcct
     2881 gtcggtgctt cctcggatgc gcagatctcg atccagacct ggtccgactc gaagaccagc
     2941 cggtcctcgt agaaacgctg ttcgcgttcc atgcgacgcc ccactccgct cgacacgttg
     3001 gctgccacca gatcgaccaa gtcgtcatag gagagatcgg caagagtgac cgtttccgga
     3061 tcatggccca agtcggcgac gacgtcctcc gggttgttct gattgaccac cgcaacaaga
     3121 ccaaacgagt cgttgatgat tgtgtagagc agtgtcacca acgcaaccac agcgatcacc
     3181 aatgagccaa ggcaggcgag ttggaaccac gtggcgcgtc gcttacgacg gctgagcagc
     3241 ccgaggtact cgtcgtcact ggggtagtgg tcagtgcgct cgcgctccgg tgtctcgacg
     3301 ctcattcgta ctcctgtcgg a
//
LOCUS       DASUTA010000177         2936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_313652, whole genome shotgun sequence.
ACCESSION   DASUTA010000177 DASUTA010000000
VERSION     DASUTA010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313652"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="VFZ80_03555"
     CDS             <1..1156
                     /locus_tag="VFZ80_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX6286537.1"
                     /translation="IQLALEETEIPLPDGVDFFFGTSVYRGTYERTGIVDATAPREVN
                     GYYWRYETAGPIEAAPVVFGTSVYVGTTEGTFFALDQTTGNEIWTMPPEGRIGAAPAL
                     GEGEMAENQAPMMIVVVDDDGAVRGHDAAVSTGVQWTTRLDSRIRSSPVIVEGRVYLA
                     TDEGFVHALDLISGDEIWRFPSDGADLGTISADLAFHNGILYVATEEGFLHLIDVAGD
                     TPSEVCQFDAFDPIVTNPIVTEDVTYVATMGQNIWPLPTGACDGNVPGRLPTYVADTP
                     VRVGPAIVGDTIYMPGGRYLYSIDLTSGEHLWEPSKVTAEAPISTPPVVTADTVYVAS
                     EDGVVRAVDAISGETLWQWQTGLHVRAAPAVVDGVVFIAAGDGYVYAVGP"
     gene            1219..>2936
                     /locus_tag="VFZ80_03560"
     CDS             1219..>2936
                     /locus_tag="VFZ80_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6286538.1"
                     /translation="MADRANVLVPYVPTAACDWDTQAPDSRWRAVEGTLVFVDISGFT
                     NLSERLAEKGRIGAEELTSVLNYVFGQMLDVVYQRGGSLLKFGGDALLLMFTTDDHVM
                     QACAATVEMRSVLRSASKEPTSVGRIDLRMSSGIHTGKIDLFLVGDSHRELIVTGPTA
                     SITTDMEATADAGEIVISRAVAESIPRSFVGDAKGNGWLLRKRKIDQPQCGWDPRSAH
                     TYDELAQFVSVGLRDHLAAGIEDSEHRIATVAFLKFKGIDEVMAALGPEEVAVMLDDL
                     VSKVQRAVDEEGITFLASDIDADGGKLIFVAGIPSSQHDDEGRVLRAARSILDSGCRL
                     PVRIGVNRGHVFSGNVGTFFRRTYTVMGDTVNLAARLMAAAHPGEVYSSPSVLSLSST
                     LFRTEALEPFYVKGKESPVQAFSVWEETGVRPPKTTSDLPFRGREAELEMLVGIVNTC
                     SRVGHGGMMTIVGATGVGKSRLIAEVLERCSGMDTLIVQAEPAGQANPYWAFRDPLRR
                     RLEIDRASQQEMTQALERKINELAPDLLQMVPLLGDVLHIAVPDNETTAAIESQFRPH
                     RTAEAL"
BASE COUNT          550 a          929 c          922 g          535 t
ORIGIN      
        1 gatccaactt gctctcgagg aaaccgagat cccccttccc gacggggtcg acttcttctt
       61 cggcacgagt gtctaccggg gcacatacga acgcaccgga atcgtcgacg ccaccgcgcc
      121 ccgggaggtc aacggctact actggagata cgagactgcc ggacccatag aggccgcacc
      181 cgtcgtgttc gggaccagcg tctacgtggg gacaacagag ggcacgttct tcgctctcga
      241 ccagaccacg ggtaacgaga tctggaccat gccaccagaa ggaaggatcg gcgctgcccc
      301 tgcgctgggc gaaggcgaga tggcagagaa ccaggcgccg atgatgatcg tcgtcgtgga
      361 cgacgatggc gcggtgcgcg gccatgacgc agcggtgtcc acgggcgtgc aatggacgac
      421 gcgactcgac agccggattc gctcatcgcc ggtgatcgtg gagggccgtg tctacctcgc
      481 caccgacgaa gggtttgtcc acgctctcga cctgatctcg ggtgacgaga tctggcgctt
      541 tcccagcgac ggggccgacc tggggacgat ctctgccgat ctcgccttcc acaacgggat
      601 tctctacgtg gcgaccgagg aaggcttcct tcatctgatc gacgtggccg gtgacacccc
      661 gagtgaggtc tgtcagttcg atgccttcga cccgatcgtg accaacccca tcgtcaccga
      721 ggacgtgacg tacgtggcga ccatgggaca gaacatctgg ccgcttccaa cgggcgcctg
      781 cgacggcaac gtaccaggcc gtctccccac ctacgttgcg gacacaccgg tacgggtggg
      841 gccggcgatc gttggtgaca ccatctacat gccgggcggc cgctatctct actccatcga
      901 tctgaccagc ggcgagcacc tctgggaacc gagcaaggtc accgccgagg ctcccatctc
      961 gacacctccg gttgtcaccg cagacactgt ctatgtcgcc tctgaagacg gggtggttcg
     1021 cgccgtcgat gcgatatccg gcgagaccct ctggcagtgg cagacaggtc tccatgtgag
     1081 ggcggcgccc gcggtcgtcg acggagtcgt attcatcgca gcaggggacg gttatgtgta
     1141 cgccgtcggg ccttgacggg ccccttccgg gaagctgccc tgctccgtct accgtgatcg
     1201 gggagcagga ggctacgagt ggctgaccgg gcaaatgtgc tcgttccgta cgtgccgacg
     1261 gcggcatgcg actgggacac acaggctccc gactcccgat ggcgcgcagt agaaggaacg
     1321 ctcgtcttcg tcgacatctc cgggttcacc aacctctctg agcgtcttgc cgaaaagggc
     1381 cgcatcggtg ccgaagagct gacctcggta ctcaactacg tctttggtca gatgctcgac
     1441 gtcgtctacc aacggggcgg atcgttgctc aagttcggtg gtgacgccct cctcttgatg
     1501 ttcaccactg acgaccatgt catgcaggcc tgcgcggcca ccgtcgagat gcgctcggta
     1561 ctgcgcagcg catccaaaga accgacctcg gtcggccgga tcgacctgcg gatgtcgtca
     1621 ggaatccaca caggaaagat cgacctcttc ctggtcgggg actcgcatcg cgagctcatc
     1681 gtcaccggtc cgacggcctc tatcaccacc gacatggagg caacagctga cgccggtgag
     1741 atcgtcatca gcagagccgt cgccgagtcc atccctcgca gcttcgtcgg cgatgccaaa
     1801 ggaaatggtt ggctgctgcg aaagcggaag atagaccaac cgcaatgcgg ctgggaccca
     1861 cggtcggccc acacctatga cgagctcgct cagttcgtct cggtgggcct acgcgatcat
     1921 ctcgctgcgg ggatcgagga ctcggagcat cgaatcgcga cggttgcctt cctcaagttc
     1981 aagggcatcg acgaggtcat ggcagcgttg gggcccgaag aagttgcggt gatgctcgac
     2041 gacctggtgt caaaggtgca acgggccgtc gacgaggagg ggatcacgtt tctcgcctcg
     2101 gacatcgacg cggatggcgg gaagctgatc ttcgtcgcag gcatcccatc gagccagcac
     2161 gacgacgagg ggcgcgtgct gcgtgccgcc cgaagcatcc tggactccgg ttgccggctg
     2221 cccgtgcgca tcggggtcaa ccgaggtcat gtcttctcgg gcaacgtggg gacgttcttt
     2281 cgacggacct acacagtgat gggcgacacc gtgaacctgg ccgcccgcct catggcggct
     2341 gctcatccag gcgaggtgta ctcgtcgccg tcagtcctca gcctctccag caccctcttc
     2401 aggaccgagg cgctcgagcc tttctacgtc aagggcaagg agagtccggt tcaggcgttt
     2461 tccgtctggg aagagacggg cgttcgtccg ccgaaaacaa cgtccgatct tccatttcgt
     2521 ggacgtgagg ctgagctcga gatgctcgtt gggattgtca acacgtgcag ccgtgtcggt
     2581 cacggaggca tgatgacgat cgtcggcgcc acgggcgtcg gtaagagccg gctcatcgcg
     2641 gaggtgctgg agcgctgctc cggaatggac accctcatcg ttcaggctga gccggctgga
     2701 caggccaacc catattgggc atttcgtgac ccattgcgac gtcggctcga gatcgatcga
     2761 gcttcgcagc aggagatgac ccaggctctc gaacgaaaaa tcaatgagct cgcccccgat
     2821 ttgcttcaga tggtgccgct gctcggagat gtcctccaca tcgcggtccc cgacaacgag
     2881 acgacggcag cgatcgaatc ccagttcaga ccccaccgca ctgccgaggc tctcat
//
LOCUS       DASUTA010000178         3814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1609743, whole genome shotgun sequence.
ACCESSION   DASUTA010000178 DASUTA010000000
VERSION     DASUTA010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609743"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3130
                     /gene="ileS"
                     /locus_tag="VFZ80_03565"
     CDS             <1..3130
                     /gene="ileS"
                     /locus_tag="VFZ80_03565"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX6286539.1"
                     /translation="PFSMTPPADDQAFSPLQGQPDHDGLEREILEWWDENGTFTKLRE
                     KNTGAEPFSFIDGPVTANKTLGVHTAWGRTLKDVFQRYKAARGHDLRYQNGFDCQGLW
                     IEVGVEQELGLNSKREIEEFGLEEFARRCRDKVAWSVQELRRGSKRLGMWMDWDHDYL
                     TFADNNIEYIWKFLRTLADRGWLYLGHRSTEWCPRCGTSISQHEITGQSGVYQEKTDP
                     SLFVRLPLLDREGDSLVIWTTTPWTLPANVAAAVHPDHEYGRRANGEWVAVERYPDEE
                     FTETLPGSELVGWRYRGPFDDLEPGSGVEHRVLAWDEVTMDQGTGVVHIAPGAGSEDF
                     ELGKEQGLAVLMPVDGAGKFYPEYGWLAGLSTVDAGDSIIADLEKKGVLVESGEYVHS
                     YPHCWRCDTPLIFRVADDWYISVEDIRQPLKDANATVEWTPTYMGKLMDDWLNNMGDW
                     NISRRRYYGLPLPFYPCDCGELTVIGSKKELEEKSTAGFDQLEELRRPWIDRVTIRCE
                     HCGNEDLHRVEEVGDVWLDAGIVPFSTLGWESPEWVSKGNATGAADGLTYADLPDHNY
                     WEKWFPADWVSEMREQVRLWFYSQLFMSVALVGRAPYTKVLGYEKMLAEDGREMHGSW
                     GNLISAEEAFEQMGADVMRWMYCAHPPDRNLLFGYQGAHEVRRRLLTLWNSARFLVDY
                     GNIEGFTPTYADLDGGPKGVELTTTDRWLLARAGQVIAESQDSLDRYRTDEQIAAFEE
                     YLDDLSNWYIRRSRRRFYEFDEAAFRTLWVGVVTALRVISPVMPFLTEHLWTNLVAGP
                     CPDAPESVHLAGWPEAPGEPDEALLAEMRAVRGVVEAGRRARMEANIKLRQPVRRIVV
                     RGADAAAGHADEIRDELRVKEISFDEETTVEVTVKPNFPVLGPRLGSKIKEVAAALER
                     GDYEDLDGGGVSAAGETLSANEVQRTEKVILEGWVVAHDGNVSVALDPLLDDGLILEG
                     KALELVRALNEQRKQEGLELTDRIALRLPVEHSEVVAEYADWIAGEVLATSVDLDDSL
                     DTPLIAKDRGLS"
     gene            3233..3679
                     /locus_tag="VFZ80_03570"
     CDS             3233..3679
                     /locus_tag="VFZ80_03570"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX6286540.1"
                     /translation="MAIADSIERIRREQGTKLIRFGAVSAFNVLFSQILLYAAQTVLD
                     MPPVGANVATVAVGTIPAYLLSRHWVWQKKGKSHWMKEVLPFWILTFIGFALSTAAVW
                     FVADRWDPAPIVINLTNLAAFGVVWLSKFVILDRILFKTEEAATAA"
BASE COUNT          695 a         1203 c         1233 g          683 t
ORIGIN      
        1 gccgttctca atgacaccgc cggcagatga ccaagcgttt tcccctctcc agggacaacc
       61 cgaccacgat gggctggagc gcgagatcct cgagtggtgg gacgagaacg ggaccttcac
      121 aaagctgagg gagaagaaca ccggcgccga gccgttcagc ttcatcgacg gccctgtgac
      181 cgccaacaag accctcggcg ttcacaccgc ttggggtcgg accctgaaag acgtcttcca
      241 gcgttacaag gcggcccgtg gccacgatct gcgctatcag aacgggttcg actgccaggg
      301 cctttggatc gaggtcggtg tggaacagga gctcgggctc aactcgaaac gagagatcga
      361 ggagttcggg ctcgaggaat tcgcccgacg ctgccgggac aaggtcgcct ggtcggttca
      421 ggagttgcgc cgcggctcga agcggctcgg catgtggatg gactgggacc acgattacct
      481 caccttcgcc gacaacaaca tcgagtacat ctggaagttc ctgcgcaccc tcgccgaccg
      541 gggttggctg tacctcggtc atcgatccac cgaatggtgc ccccgatgcg gaacatcgat
      601 atcccagcac gagatcacag gtcagtcggg ggtctatcag gagaagaccg acccttccct
      661 gttcgtccga ttgcccctcc tcgaccggga aggcgacagc ttggtcatct ggaccacgac
      721 gccgtggacc ctccccgcca acgtggccgc agcagtccat ccggatcacg aatacgggcg
      781 cagagccaac ggtgaatggg tagccgtcga gcggtatccc gatgaggagt tcacggagac
      841 ccttcccggg tccgaactgg tgggctggag ataccgcggt cccttcgacg acctggagcc
      901 cggatcgggg gtggagcatc gggttctcgc ctgggatgaa gtcacaatgg atcagggcac
      961 cggcgttgtc cacatcgccc ctggcgccgg ctcggaggac ttcgagctcg gcaaggagca
     1021 gggtctggct gtcctcatgc ccgtggacgg ggcggggaag ttctatcccg agtatggctg
     1081 gctcgccggc ctgtccaccg tcgatgccgg ggattcgata atcgccgacc tggagaagaa
     1141 gggagttctc gtcgagtccg gcgagtacgt gcactcctat ccgcattgct ggcgatgcga
     1201 cacgcctctc atcttccggg tggcagatga ctggtacatc tcggtcgaag acataaggca
     1261 gcctttgaag gacgccaacg ccaccgtcga gtggacgccg acgtacatgg gcaagctcat
     1321 ggacgactgg ctgaacaaca tgggcgactg gaacatctcc cgtcgccgct actacggctt
     1381 gccgcttccc ttctacccct gcgactgcgg tgagctcacc gtgatcgggt ccaagaagga
     1441 gctcgaggag aagtccaccg caggcttcga ccagctcgag gagctgcgac gcccctggat
     1501 cgatcgggtg accatccgtt gcgagcactg tggcaacgaa gacctccatc gtgtcgagga
     1561 ggtgggtgac gtctggctcg acgccgggat cgtccccttc tccacgctcg gttgggagag
     1621 cccagagtgg gtctccaagg gcaacgccac cggagcggcc gacgggctca cttatgccga
     1681 tctccccgat cacaactact gggagaagtg gttcccggcg gactgggtgt cggagatgcg
     1741 tgagcaggtg cgtctctggt tctactcaca gctgttcatg tcggtggcgc tggtggggag
     1801 ggccccgtac acaaaggtgc tcgggtacga gaagatgctc gccgaggacg gccgggagat
     1861 gcacgggtca tggggcaacc tgatcagcgc cgaggaggca ttcgagcaga tgggggccga
     1921 cgtgatgcgc tggatgtact gcgcccaccc accggaccgc aatctcctgt tcggctatca
     1981 gggtgcccat gaggttcgtc gccgcctcct caccctgtgg aactcggctc gtttcctggt
     2041 ggactacggc aacatcgagg ggttcacccc cacctacgcc gacctcgatg ggggcccgaa
     2101 aggtgtcgag ctcaccacca cagaccgctg gctgctggct cgggccggac aagtcatcgc
     2161 tgagtcgcaa gacagtctcg atcgatatcg caccgacgag cagatcgctg cgttcgagga
     2221 gtatctcgac gatctctcca actggtacat ccggcgcagc cgacggcggt tctacgagtt
     2281 cgacgaggcg gcttttcgca ccctgtgggt cggggtggtc accgccctga gggtcatctc
     2341 ccccgtcatg cccttcctga cggagcacct gtggacgaac ctcgtcgccg ggccctgccc
     2401 cgacgcaccg gagtcggtgc acctggccgg atggccggag gcgcccggtg agccagacga
     2461 ggcgctgctc gccgagatgc gagcggtgcg tggcgtagtc gaggcgggtc gtcgggcccg
     2521 gatggaggcg aacatcaagc ttcgccagcc ggtgcgccgc atcgtggtcc gtggcgccga
     2581 tgccgccgcc ggccacgccg atgagatccg agatgagctt cgagtcaaag agatcagctt
     2641 cgacgaggag accaccgtgg aggtgacggt caagccgaac ttccccgtcc tcggccctcg
     2701 tctcggctcc aagatcaaag aggtcgcggc cgctctcgag agaggggact acgaagacct
     2761 cgacggtggc ggggtgtcgg ctgctggcga gacgctctcc gccaacgagg tgcagcgaac
     2821 ggagaaggtg atcctcgagg ggtgggtggt ggctcacgat ggaaacgtca gcgtcgccct
     2881 cgatcccctc ctcgatgacg gcctgatcct ggaggggaag gccctcgaat tggtgcgtgc
     2941 cctcaacgaa cagcggaaac aggagggtct cgagctcacc gatcggatag ccctccgcct
     3001 cccggtagag cactcagagg tggtcgccga atacgccgat tggatcgccg gcgaggtcct
     3061 ggcgacctcg gtcgacctag acgactctct cgacactccc ctgatcgcga aggaccgggg
     3121 cctctcctga cgcgacctcc ggtagttaga gccgtatacg gctctaacta ccggaggtcg
     3181 cgggatccgc ttgggtctag gccgcgatct gccgatacca tcggtcgtgc caatggccat
     3241 cgcagactcc atcgagcgaa tccgacggga acaggggacg aaactcatcc gcttcggtgc
     3301 ggtgagcgcg ttcaacgtcc tgttcagcca gatcctcctc tacgcggctc agaccgtcct
     3361 cgacatgccg ccggtcgggg caaacgtcgc cacggtggcc gtgggaacca tcccggccta
     3421 cctcttgagc cgtcactggg tctggcagaa gaaaggcaag agccactgga tgaaggaggt
     3481 cctcccgttc tggatcctca ctttcatcgg gttcgccctc tccaccgcag cagtgtggtt
     3541 cgtggccgat cgatgggacc ccgcacccat agtcatcaac ctgaccaacc tcgcggcctt
     3601 tggcgtcgtg tggctgtcca agttcgtgat cctcgatcgg atcctcttca agaccgagga
     3661 ggccgccacc gcggcttgag gctcaggcct cgtcttcgtc gctcgactcg ctgctgtggt
     3721 cgctctcgtc gtcgtcgtcc tggtcatcct cggtgtcatc ccctgagtcg ccgagctata
     3781 aatccgtggc gaccccagtg aggatggcgt cgag
//
LOCUS       DASUTA010000179         2280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_831148, whole genome shotgun sequence.
ACCESSION   DASUTA010000179 DASUTA010000000
VERSION     DASUTA010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831148"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1029)
                     /locus_tag="VFZ80_03575"
     CDS             complement(142..1029)
                     /locus_tag="VFZ80_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286541.1"
                     /translation="MKVPMVCLFALLLVVSCSAEPEVSREELVQLVETEGAAFSDSRL
                     SSEVLDDLASHRVVVLGETHRLREHWEFLATLVEALHGRGFRQVLIEAPHMAEWLYDD
                     YVHGGVLVPGLELPKFYEDRLAGIRAVNEGLEPGERIRVRAIDVNEEHHGGGQAFRDV
                     LGSLVSILGEPGPVEAFTATTAGSPEEQRESIERLSASLRSESSTLSEEWGSEWFAHV
                     LEMVEVERASIDIREYRQSDDDRAARAREEEIKELVESRLTDVEGGTVINVGEHHAQK
                     SPLMGTDQEWLGDYLIHRS"
     gene            complement(1481..>2280)
                     /locus_tag="VFZ80_03580"
     CDS             complement(1481..>2280)
                     /locus_tag="VFZ80_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX6286542.1"
                     /translation="LSPFVEYVDPDNAGYDAGFGAFALETWWPPAPGPTWFLGVLLVF
                     SLGYALLRTLWPQRATKMSSPHLARLTIFVGLVAAISYLVRLAIPMGVEVWRLALGQA
                     PGWLAGFGLGVYGAERGWFDPIDPPIARTMRRTAWAATAFLVLFIGAAMATGGNIEDF
                     AGGGTWQSLVIAGVEATLIVAMSLWILDLFHRRFDHQGPLAQELSRSAYAAFVLHQVV
                     LVGLVLITHDTTWSPEIEFALVAGLGVVGSFGLASIFIRLPGVNRVL"
BASE COUNT          483 a          740 c          644 g          413 t
ORIGIN      
        1 cggccaggtc tcggccatca gccggagcac ctcatcttcg ggagatgact gcacgatgtt
       61 ggatggaata cctggcgctc ctggttccag aaccgtctct gccgacgtga acccgatgat
      121 gaagaccgaa ccctctgccg cctagctccg atggatcaga tagtcgccga gccactcctg
      181 gtcggttccc ataagaggtg atttttgggc atggtgctcc ccaacgttga tcaccgtgcc
      241 cccctcgacg tctgtcagcc tggattccac tagctccttg atctcctcct cgcgggcgcg
      301 ggcagctcga tcgtcatcgg actgcctgta ctccctgatg tcgatactgg cccgttcgac
      361 ctcgaccatc tccaacacgt gtgcaaacca ctcggaaccc cactcctcac tgagagtcga
      421 cgactcagag cgcagtgacg cactcaaccg ctctatggac tcacgttgct cctccggtga
      481 tccagcagtt gttgcggtga acgcctctac cggcccgggc tctccgagga tgctgacgag
      541 cgatccaagg acgtctctga atgcctgccc tcctccgtga tgctcctcgt tgacatcgat
      601 cgcgcgaact cggatccgtt cgccgggctc aagcccttcg ttcactgccc gaatcccggc
      661 tagacgatct tcgtagaatt tgggcagctc caacccgggt accagaaccc caccgtggac
      721 atagtcgtcg tagagccatt cggccatgtg gggcgcctcg atcaatacct gacgaaagcc
      781 ccgcccgtgg agggcctcga ccaacgtcgc cagaaactcc caatgctctc gcagacgatg
      841 cgtctcgccc agcaccacaa cgcggtgaga agccagatca tcgaggacct ccgaagacag
      901 gcgcgagtcc gagaaggcag ccccctccgt ctcgacgagc tgaactagct cctctctgga
      961 gacttcgggt tcggcactgc aggagacgac caagagcagg gcaaacaggc acaccatcgg
     1021 caccttcatt tcgagaaggc gacgcactag ccctcactcc tactacagct ggcaccacat
     1081 ctaccttcat cgggcatatc cgggctagag acaaaagtcc cgatgctgcc tacaactcat
     1141 cggtgacgtg gccattcaaa cccccggcat attcgcacca ctcacttcgc ccatcctcgc
     1201 cttcgcatcg gtttcaatgg ccccggggcc gtcaccagcg gattcggtgg ccccggaaca
     1261 cgaaatcggt gcagtgatgg cagaaatgcc aatacctatg ggcgacttcc gtggtcgact
     1321 gcgggacacg tcaaacgcat gtcaaacgtc ctttcggctt tcaagttcgg ctttcacgaa
     1381 gacgcgtcaa gcggcgccgc tcccccgctc ggtatgtgcc tgattgatga tgtgggtggc
     1441 tgcggagcgt gtgttcagca gtgtgcccaa tggtgaacgg ttagagcacc cggttcactc
     1501 cgggcagtct gatgaagatc gatgccagcc cgaagctgcc gacgacgccg agtccggcga
     1561 ccaacgcgaa ctcgatttct ggagaccaag ttgtgtcgtg ggtgataagc accagtccca
     1621 cgaggacaac ctggtggaga acaaacgcgg cgtaggcgga gcggctcaac tcctgagcca
     1681 ggggaccttg atggtcgaag cgtcggtgga agaggtcgag aatccaaagt gacatggcca
     1741 cgatgagagt cgcttcgact ccggcgatga ccagtgactg ccaggttccg cccccggcga
     1801 agtcttcgat gttgccgcca gttgccatcg ccgcgccgat gaacaagaca aggaaggcgg
     1861 tggcggccca tgctgtccgt cgcatggtac gtgcgatggg tgggtcgatc ggatcgaacc
     1921 agcctcgttc cgcgccatac acaccgagac cgaaaccggc aagccacccc ggcgcctgtc
     1981 ccaacgccag acgccacact tcgactccca tcgggatggc cagcctgacc agatacgaga
     2041 tcgctgcaac aagcccaaca aatatggtca gccgagcgag atgaggcgac gacatcttcg
     2101 tggcccgctg cggccagagt gttcgcaaca gggcatagcc aagcgagaag accagaagca
     2161 caccgagaaa ccaggttggc ccgggagctg gcggccacca ggtttcaaga gcgaaggcgc
     2221 cgaacccggc gtcatatccg gcgttgtccg ggtcgacgta ttccacgaac ggactcagga
//
LOCUS       DASUTA010000180         3682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1091822, whole genome shotgun sequence.
ACCESSION   DASUTA010000180 DASUTA010000000
VERSION     DASUTA010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091822"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1100
                     /locus_tag="VFZ80_03585"
     CDS             144..1100
                     /locus_tag="VFZ80_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6286543.1"
                     /translation="MTELRGKHAVVLGGAGFLGSHVCSALIEADCSVTCLDNMITGSP
                     SNVDHLLNGGARFRLIDYDVTDFIHVSGEVDYVLNFASPASPVDYLKWPIHTLKTGAF
                     GTHKGLGLALAKGAVFLHASTSEVYGDPQVNPQPESYWGNVNPIGPRGVYDEAKRFAE
                     ALTLAYHREHGLETRIVRIFNTYGPRMRPDDGRAVPAFFKAALENRPLPVHGDGNQTR
                     SLCYVDDEVEGILRLLLSSEHDPVNIGNPEEVTINELAEAVQDVVGNYPGIDHRPRPT
                     DDPNMRRPDTTRAEELLGWKAQISLRAGLQRTASWFRHVLGY"
     gene            1150..2298
                     /locus_tag="VFZ80_03590"
     CDS             1150..2298
                     /locus_tag="VFZ80_03590"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011605703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX6286544.1"
                     /translation="MPLDSSVASRYLTLDDVEVSGKRLLVRSDLNVPLEGTEVADDFR
                     IRAALPTLERLVGDGADVTVCSHLGRPKGEDPALSMRTVAEAMDAITDLDVGFESGGE
                     ITVLENTRFDEGETSNDKDTAKRLADGFDLFVQDAFGSVHRAHASTVGVAELIQSVAG
                     PLLDRELTSLGRFLTDTEHPYTVVLGGAKVSDKLAVIENLLPRVDRMLIGGGMCFTFL
                     AALGHNVGESLVEEAMLDKVAELAEDDRIALPTDVVIADSFRKNADHAITVVSEIPDG
                     WMGLDIGTDTAIDFANLVEDSASVFWNGPMGVFEWEAFRNGTELVARALADCDGFTAV
                     GGGDSVAALRALGLEDSVSHLSTGGGAGLELLEGKILPGVAVLERWAE"
     gene            2295..3068
                     /gene="tpiA"
                     /locus_tag="VFZ80_03595"
     CDS             2295..3068
                     /gene="tpiA"
                     /locus_tag="VFZ80_03595"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX6286545.1"
                     /translation="MTERAPLIVGNWKMNANHLEAIQMVQKLSYRLEAEDYERVDVVV
                     APPFTSLRSIQVVIEQDRIPIALGAQDVFWAESGAYTGEISAPMLAKLAVKHVIVGHS
                     ERREHFGETDEMVNRKAKAVIAAGMAAIVCVGETLDQREAGEAESVVTTQLAGALRGL
                     DLEDPASLAVAYEPVWAIGTGRTALPDDAATIATIVREQLRDTLNSEAGEQVRVLYGG
                     SVNAVNIKDFMAKKDIDGALVGGASLDPDQFAAIVRYRL"
     gene            3065..>3682
                     /gene="gpmA"
                     /locus_tag="VFZ80_03600"
     CDS             3065..>3682
                     /gene="gpmA"
                     /locus_tag="VFZ80_03600"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017882864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEX6286546.1"
                     /translation="MSHKLVLLRHGQSEWNLSNLFTGWTDVGLTEEGEEEAKEAGRLM
                     AEAGLKPDIMFTSVLSRATDTADIALEEAGWTDIPTKRSWRLNERHYGDLQGLNKKET
                     AEKFGDDQVLIWRRSYDTPPPALDLSDERHPSRDPLYADIDPSEIPSTEALSNVVDRM
                     LPYWFDEIVPELQAGKTVLVVAHGNSIRALVKHLDDVSEEDIVGLN"
BASE COUNT          717 a         1157 c         1146 g          662 t
ORIGIN      
        1 tagcgccagg agttgaacca aggcgcgata gctcccctcc gaagtaggtc tacagaccta
       61 ggcgagtaac cgcgggcgaa atgtacgatg tcactgtggg tggcgaagtt caaagagacg
      121 gcggtgggca acgtccgttg gcaatgaccg agctccgtgg caagcatgcc gtggttcttg
      181 gaggcgcggg cttcctcggg tctcacgttt gttcagccct catcgaagcc gactgctcgg
      241 tgacatgtct ggacaacatg atcactggct cgcctagcaa cgtcgaccac cttctcaacg
      301 gcggcgcacg attccgcctc atcgactacg acgtgaccga cttcattcat gtgtccggtg
      361 aggtcgatta tgtgttgaac ttcgcgtctc cggcatcccc tgttgactac ttgaagtggc
      421 cgatccacac cttgaagacc ggtgccttcg gcacacataa aggtttaggg ctagcgctcg
      481 cgaagggcgc tgttttcctt cacgcttcca catccgaggt gtatggcgac ccgcaggtga
      541 acccacagcc agagtcctac tggggcaacg tgaatccgat cggaccgcgc ggtgtttacg
      601 acgaagcgaa gagatttgcc gaggccctca ccctcgccta ccaccgcgag cacggtctcg
      661 agaccaggat cgttcgcatc ttcaacacct acggcccaag gatgcggccc gacgacgggc
      721 gcgccgtgcc ggccttcttc aaagccgccc tcgagaaccg gccgctgcca gtgcatggcg
      781 atggcaacca gacccgatcc ctgtgctacg tcgatgacga ggttgaggga atccttcggt
      841 tgctgctctc ctccgaacac gacccggtga acatcggtaa ccccgaagag gtcactatca
      901 acgagttggc cgaggcggtg caagacgtcg tcggcaacta cccggggatc gaccatcgac
      961 cgcgacccac tgacgacccc aacatgcgca gacctgacac gaccagagct gaagaactgc
     1021 tcggctggaa ggcacagata tcgctacggg ctgggttgca gcggaccgcc tcctggttcc
     1081 gtcacgtcct cggctactaa cacattcagc catcacccgc ccgatccgcc cgaccctaag
     1141 ctcggagcga tgcccctcga ttcatcggtt gcctccaggt acctgacgct cgacgatgtc
     1201 gaggtttccg gcaagcgcct cctggtgcgc tccgatctca atgtgccgct cgagggcacc
     1261 gaggtcgccg acgacttcag gatcagggcg gctcttccca ccctggagcg tctcgtcggc
     1321 gacggagccg atgtgacggt gtgctcccat ctgggtcggc ccaaaggaga agacccggcg
     1381 ctctccatgc ggaccgttgc cgaggcgatg gatgccatca ccgatctcga tgtggggttc
     1441 gagtccggcg gggagatcac ggtgctggag aacacccgtt tcgacgaggg tgagacatcg
     1501 aacgacaagg acacggcgaa acggttggcc gacgggttcg atctcttcgt ccaggacgcc
     1561 ttcgggtcgg tgcaccgcgc ccacgcttcc acggtggggg tcgccgagtt gatccaatcg
     1621 gtggccggcc ccctcctcga cagagagctg acatcactgg gacgtttcct caccgatacc
     1681 gagcacccct acacggtcgt gctcggcggg gccaaggtct ccgataagct cgcggtgatc
     1741 gagaacctgc tgccccgggt cgaccggatg ctcatcggcg gcggcatgtg cttcacattt
     1801 ctcgccgccc tcgggcacaa cgtcggcgaa tcgctggtcg aggaggcaat gctggacaag
     1861 gttgccgagc tcgccgagga cgatcgcatc gccctcccca ccgatgtggt catcgccgac
     1921 agtttcagga aaaacgccga ccatgcgata accgtggtga gcgagattcc cgacgggtgg
     1981 atgggtctcg acatcgggac cgacaccgct atcgacttcg ccaacctcgt cgaagactcg
     2041 gcgagcgtct tctggaacgg gcccatgggt gtcttcgagt gggaggcctt tcgcaacggt
     2101 accgagctcg tcgcccgtgc cctcgccgac tgtgacgggt tcaccgcggt tggcggaggc
     2161 gattcggtcg ccgcattgcg cgcgttgggt ctcgaggact ccgtgagcca tctctccacg
     2221 ggcgggggag ccggcctcga gctgctggag ggcaagattc tgcccggtgt cgccgttctg
     2281 gaaagatggg ctgaatgacc gaacgcgccc cgctcatcgt cggcaactgg aagatgaacg
     2341 ccaaccacct ggaggcgatc cagatggtgc agaagctctc ctaccgactc gaggccgagg
     2401 actacgagcg agtcgacgtg gtggtggcgc ctccattcac ctcactgcgc tccatccagg
     2461 tggtcatcga acaggaccgg atccccatcg ccctgggcgc acaggatgtg ttctgggccg
     2521 aatccggcgc ctatacgggt gagatctcgg ccccgatgct ggccaagctg gctgtgaagc
     2581 acgtcatcgt cggccactcc gagcgtcgcg agcacttcgg cgagaccgac gagatggtca
     2641 acaggaaggc caaagcggtc atcgcagccg gcatggccgc catcgtctgt gtcggggaga
     2701 ctctggatca acgtgaggca ggtgaggccg agtcggttgt cactacccag ttggccgggg
     2761 cattgcgtgg tctggatctc gaagatccgg catcactcgc ggtcgcctac gaaccggtgt
     2821 gggcgatcgg taccggtcgg acggccctcc cagatgacgc cgccaccatc gcgactatcg
     2881 tccgtgaaca gctccgggac accctcaaca gcgaggcggg agagcaggtg cgggtcctct
     2941 acggaggctc ggtcaatgcg gtcaacatca aggacttcat ggccaagaag gacatcgatg
     3001 gcgccctggt gggtggcgct tccctcgacc cagaccagtt cgccgccatc gtgaggtacc
     3061 gactgtgagt cacaaactcg ttcttctccg ccacggccaa tcggaatgga atctgtccaa
     3121 cctgttcacc gggtggaccg atgtcggtct aactgaggaa ggggaggagg aggcaaaaga
     3181 ggccggacgc ctgatggcgg aggccgggct gaagcccgac atcatgttca cctcggtcct
     3241 gagccgggcc accgacacag ccgacatcgc cctcgaggag gccggctgga cagacatccc
     3301 caccaagcgg tcctggcgac taaacgagcg tcactatgga gatcttcagg gactcaacaa
     3361 gaaagagaca gccgagaagt tcggcgacga ccaggtgctg atctggagaa gatcctacga
     3421 caccccgcct ccggctctcg atctctccga cgagagacac ccgtcacgtg atcccttgta
     3481 tgccgacatc gatccgtccg agatcccgtc gaccgaggcg ctgtccaatg tggtcgatcg
     3541 gatgcttccc tactggttcg atgagatcgt gcccgaactg caagcgggca aaacggtgct
     3601 ggtggtggct cacggcaact cgatccgggc gctggtcaag catctggacg atgtcagcga
     3661 ggaagatatc gtcgggttga ac
//
LOCUS       DASUTA010000181         4162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1610607, whole genome shotgun sequence.
ACCESSION   DASUTA010000181 DASUTA010000000
VERSION     DASUTA010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610607"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VFZ80_03605"
     CDS             complement(<1..1045)
                     /locus_tag="VFZ80_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HEX6286547.1"
                     /translation="MAKPSGAICNLDCTYCFFLSKEMLYPGDRFRMADELLETYLRQL
                     IESTPYPEVTIAWQGGEPTLMGVAFFERAVELANRHVRAGQRLQFTIQTNGTLLNDKW
                     GEFLARHRFLVGLSVDGPREMHDAYRVDKKGQGSFDRVMRGFEVLQRHGVDFNILCTV
                     HAANGDHPAEVYRFFRDDLGATFLQFIPIVERTSPDLIQIANIGWGSKNTKRPLYTNT
                     GSQVTNRSVKPEQWGRFLSGIFDEWVNNDVGDVFVQHFDAALASWLGIEPSLCIFRET
                     CGTAIALEHNGDVYSCDHFVEPDYLLGNIGDTHLSALVTSDQQKSFGEAKRDTLPQYC
                     LDCDVRFACNGECP"
     gene            1269..1997
                     /locus_tag="VFZ80_03610"
     CDS             1269..1997
                     /locus_tag="VFZ80_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286548.1"
                     /translation="MSPLLAQEIPEGLNVRRAAKLTAWLGIAYSLLVLGSYYMLSLAP
                     RPSSSDEEYLAFYDSDETRVILIAGLYLMPFAGIAFMWFIVMLRAWIRRTASRVDDLF
                     SSVQLVSGILYLALFLTAAAAISVLSAGGGSPEAAIDPSTKRQFTQMGWGLVVVFAMR
                     MAAIFVITTTTLGRRHRFLPQWFVILGYLVGAFLLLSANTAPFLILVLPGWTLLLGLL
                     LLARARSLPTTVGPTPSSPSEDGA"
     gene            complement(2024..>4162)
                     /locus_tag="VFZ80_03615"
     CDS             complement(2024..>4162)
                     /locus_tag="VFZ80_03615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEX6286549.1"
                     /translation="YCVGLIVAMSIGGRRLRKALRDVVIASVGSVLLTVVVIQIINGE
                     LPPALLEFGFGDPDPHFPILRVVAVVATLISASPHLTRPMRRLGWVAVALTTTAAVAL
                     GYGDPSDALGAMGIGMLAAGSVLLVAGSPRGYPDPHEVAEALNGMGLGVDEVRIDPEQ
                     TWGVRRLVATTGDGSILAIKAYGRDASDSLRIAKAWRTLWYRERGRAVSFSRLQAVEH
                     EALVTVMAGRTDVRVSDVIAAGEESSRMALLVQPGGIPTLRGLDAEAIDEDLLVRIWE
                     DVERLHDASISHGALTTEAILVDRSGHRFSDFALGSLVADDSERLQDTVELLFSLALL
                     VGSARAVSSAVSGLGVDRLAAALPYLQLPAVSRGTRRQADSPKDLMTELQVAVTEATG
                     IDMPEPVKLRRVSGRNVATAALLILAAGALIPMLAGIDFAEVWAVLEDASWGIVLLAL
                     AVGQAMFIPEAMGMMYAVGGKLPFWPLVTLQVGAKFISLAVPTFAGRVAMNSAFLHKF
                     GMSTTLAVTQGAIDGFGGFLVEVAILVLAIFAGDLDLGLGFDSGEVSWGVVLLVVAGL
                     ALATVAAFRRVKRLREKVLPRIEDAWATLSDLLKEPGRALGLLGSNLATRLLMAGSLW
                     LALAAIDSPISFAGCLIAVVATNLLQGLIPVPGGIGVTETVMTGFLVGLGVDEASAFA
                     ATITWRVITFYLPSIEGFFAMRWLERHEYL"
BASE COUNT          800 a         1338 c         1261 g          763 t
ORIGIN      
        1 tggggcactc gccgttgcag gcgaagcgca cgtcgcaatc gagacagtat tgggggaggg
       61 tgtctcgttt cgcctcgccg aagctcttct gctggtcgga ggtgacgagt gcggagaggt
      121 gggtgtcacc tatgttgccg aggaggtagt ccggctcgac gaaatggtca cacgagtaga
      181 cgtcaccgtt gtgttcgagg gcgatggcgg tgccgcatgt ctctcggaag atgcacagcg
      241 acggctcgat gcctagccat gaggcgagcg cggcgtcgaa gtgttgtacg aagacgtcgc
      301 caacgtcgtt gttcacccat tcgtcgaaaa tcccgctgag aaaccggccc cactgttccg
      361 gcttcaccga ccggttggtc acctgtgagc cggtgttggt gtataggggg cgtttggtgt
      421 tcttcgagcc ccagccgatg tttgcgatct gaatgaggtc ggggctggtt cgctccacga
      481 tggggatgaa ctggaggaaa gtggctccga ggtcgtcgcg gaagaagcgg taaacctccg
      541 caggatgatc gccgttggcg gcatggaccg tgcacaggat gttgaaatcg acaccgtgcc
      601 gttgcagcac ctcgaatcca cgcatcacac ggtcgaagct cccctgaccc ttcttgtcga
      661 cccgataggc atcatgcatc tcgcgtgggc cgtcgacgga caggccgacg aggaaccggt
      721 gacgagccag gaactcaccc cacttgtcgt tgaggagcgt tccgttcgtc tggatggtga
      781 attgaaggcg ctgtccagcg cggacatgcc ggttggcgag ctccaccgcc cgttcgaaga
      841 aggctacgcc catcagggtg ggctctccgc cctgccaggc aatggtcacc tcgggataag
      901 gagtcgattc gatcagttgc cgcaggtaag tctcgaggag ctcgtcagcc atgcggaaac
      961 ggtcaccggg atagagcatc tccttggaca ggaagaagca ataggtgcag tccagattgc
     1021 agatcgcccc agatggtttt gccatcacat gaaacggtga tggggcggtt gcggtaatca
     1081 ttctcctcgc ctcacccgga gctgtgggac cgctccggcg taatgtatga cactccccga
     1141 ttcaatccct agcggttacc ttcctcatca cccagcgcgg gtgattcacg atcatccaga
     1201 cagggggatg tcgtcgctcc ggcaacgtca caccatcgaa gtggtaagtc ggaggaagag
     1261 agccgccctt gtcaccactg cttgcacagg agattccgga aggcttgaac gtccgtcggg
     1321 cggcgaagct gaccgcgtgg ctcggaatcg cgtacagcct gctcgtattg ggttcctatt
     1381 acatgctcag cctcgctcct cggccttcgt cgagtgatga ggagtacctg gcgttctacg
     1441 actcggatga gacgcgggtc atcctgattg ccggcctcta cctgatgccg ttcgccggca
     1501 tcgcgttcat gtggttcatc gtcatgctcc gagcgtggat aagacgaacg gccagcaggg
     1561 tcgacgacct cttctccagt gtccaactgg tgagtgggat tctgtatctg gcgttgtttc
     1621 tcaccgctgc ggccgcgatc tctgtcctgt ctgcgggcgg tggctcccct gaagcggcca
     1681 tcgacccgtc gacgaagcgg caattcaccc agatgggctg gggattggtg gtggtatttg
     1741 ccatgcgaat ggccgcgata ttcgtgatca cgactaccac cctgggtcgc cgacatcgat
     1801 ttctccccca gtggttcgtc atcctcggct atctggtcgg cgcctttctc ctgctgagcg
     1861 ccaacacggc cccgttcctg atcctcgtct tgcccggctg gactctgctg ctcggattgc
     1921 tcctgttggc tcgggcgcgg tccttaccga ccaccgtcgg gcccacccct tcgtcgccaa
     1981 gtgaagacgg agcctgatcg cccggacttg ttgctccaat aggtcacaaa tactcgtggc
     2041 gctccagcca tcgcatcgcg aagaacccct cgatcgacgg taggtagaaa gtgatcaccc
     2101 gccaggtgat cgtggcggcg aaggcggaag cctcgtccac ccccaggcca acgagaaagc
     2161 cggtcatcac cgtctcggtg actccaatcc cacccggcac aggtatcagg ccctggagca
     2221 gattcgtcgc cacgaccgcg atcagacatc cggcaaagct gatcggactg tcgatcgccg
     2281 ccagcgccag ccacagagac cccgccatca gcagtcttgt cgccagattg ctacccagca
     2341 ggcccagcgc ccttcctggt tccttgagca ggtcggagag ggtggcccag gcgtcctcga
     2401 tccgcggcaa taccttctca cgaagcctct tgaccctccg gaacgcagcg accgtcgcca
     2461 gcgccagccc ggccacgacc aacaggacca ctccccaact cacctcgcca ctgtcgaacc
     2521 caaggccgag atcgagatca ccggcgaata tcgccaggac caggatggcc acctcgacca
     2581 ggaaaccgcc gaaaccgtcg atcgcgccct gggtcacggc caacgtcgtg ctcatcccga
     2641 acttgtggag aaaggccgag ttcatcgcga cccttccggc gaacgtgggg acggccagac
     2701 tgatgaattt tgctcccacc tgcaacgtga cgagtggcca gaaggggagc tttcccccca
     2761 ccgcatacat catccccatg gcttccggga tgaacatcgc ctgtccgaca gccagcgcca
     2821 agagtacgat cccccaggaa gcgtcctcca gtactgccca cacctcggcg aagtcgattc
     2881 cggcgagcat cgggatcaag gccccggcgg ccaggatcag caaggcggcc gtggcgacat
     2941 tcctgccgga gacgcgtcgg agcttcacgg gctccggcat gtcgattccg gtcgcttcgg
     3001 tgacggccac ctgcagctcg gtcatcaggt ccttcgggct gtccgcctgt cggcgcgtgc
     3061 cgcgactcac cgccggcagc tgcaggtagg ggagcgcggc tgcgagtctg tcgacaccca
     3121 atcccgacac ggccgagctg acggcgcggg cggagccgac gaggagggcc aaagagaaca
     3181 gcagctcgac agtgtcctga agacgctcgg aatcgtccgc aactagggac ccgagcgcga
     3241 agtcgctgaa cctgtggcca gaccggtcga ccaagatcgc ctccgttgtc agcgccccat
     3301 ggctgatcga ggcgtcgtga agtcgctcca cgtcctccca gatccggacc agtaagtcct
     3361 cgtcgatcgc ttcggcatcg agtcctcgca gcgtggggat gccgcctggc tggaccagaa
     3421 gcgccattct tgaggattcc tcgccagcgg caattacatc tgacactcgg acatccgtgc
     3481 ggcccgccat cacggtgacc agcgcctcat gctcgactgc ctggagtctc gagaagctga
     3541 ccgcacggcc tcgttcccga taccaaaggg tccgccacgc cttggcgatc cgcagactgt
     3601 cggacgcatc gcgtccgtag gccttgattg caagaatcga accgtctccg gtagtggcga
     3661 cgagacgtct cacaccccat gtctgctcgg gatcgatcct gacctcgtcg acacccaggc
     3721 ccatgccgtt caaggcctcg gcgacctcat gcgggtccgg gtagccacgg ggcgacccgg
     3781 cgacgagtag aacggaaccg gccgccagca tcccgattcc catcgcgcct aaggcgtccg
     3841 atggatcgcc gtagcccaac gcaaccgccg cggtcgtggt gagtgccacc gccacccagc
     3901 cgagacgccg catcggtcta gtgagatgtg gtgaggcaga tatcagggtc gccaccacag
     3961 cgacgacgcg caagatcgga aaatgcgggt ctggatctcc gaagccgaat tcgagaagcg
     4021 caggcggcaa ttcgccattg atgatctgga tgaccacgac cgtcagcagg accgagccaa
     4081 ctgacgcaat gaccacatcc cggagcgcct ttcgaagccg gcggccgcca atggacatcg
     4141 caacgatcag acccacgcag ta
//
LOCUS       DASUTA010000182         7266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_702947, whole genome shotgun sequence.
ACCESSION   DASUTA010000182 DASUTA010000000
VERSION     DASUTA010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7266
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702947"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..641
                     /locus_tag="VFZ80_03620"
     CDS             168..641
                     /locus_tag="VFZ80_03620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6286550.1"
                     /translation="MKPSDWPGVARVYAEGISTGMATFETSVPGWDDWDAGHIEACRL
                     LVEADGEIVAWAALSPTSRRDVYRGVAEASIYVGEKARGKGIGSALLGELVSCSEREG
                     YWTLQAGIFPENEASVRLHQRHGFRVVGTRERIGASNGRWRDVLLLERRSETIGQ"
     gene            complement(941..2584)
                     /locus_tag="VFZ80_03625"
     CDS             complement(941..2584)
                     /locus_tag="VFZ80_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015788119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6286551.1"
                     /translation="MSHTHEVLNQVPPLEGYNLYTSDPVLDEAVGREGAKGSRDRLIE
                     FGDLTGSVEYYRWGFDANRNDPELITHDRHGHRVDRVEFHPSWHRLMQTAVANGNHSL
                     PWEEEAVEGAHVARAVFTYLTGQIESGHNCPISMTYSVIPALQTTPEVGREWIPGILS
                     HDYDPSFRPAKEKQGLLMGMGMTEKQGGSDVRANTTEAVPLNGGGTGAEYAITGHKWF
                     TSAPMCDAFLVLAQAPGGLSCFLLPRWAPDGRLNAFHIQRLKDKLGNRSNASSEVEFD
                     AAWAVMVGDEGRGVPTIIEMVNGTRLDCVIGSAALMRQAVSQAAWHVSHRAAFGSKLI
                     DKPLMRNVVADLEVETEAATEMMIRLAGAFDRSEVDPSEAAMKRIALPVAKYWVTKRT
                     SEVVREALECLGGNGYVEESIMPRLYRESPLNAIWEGSGNVIALDVLRAVGRSPDSLE
                     MFLTEIDQASGMDTRLDFAIEAARTALTTATDIEFQARRIVESMAVAWAGSLLARQGE
                     PAIFEAFASSRLGERHGGLYGTLPPGTSVETLLERATPA"
     gene            2707..5586
                     /locus_tag="VFZ80_03630"
     CDS             2707..5586
                     /locus_tag="VFZ80_03630"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX6286552.1"
                     /translation="MRDRIRGCAVGAALVVVVTACTGSADPTTTTAHAVPTTSVLAST
                     TTTEDPILNLALPVDPDVVQGVLDNGMTYYIRENDSPGGRAELRLLVDAGSVQEDPDQ
                     AGMAHFLEHMMFNGTENYPRNDLIDVLEAFGPRFGPDINAFTSYDETVYELSLTTDNP
                     ELLVLGVDVLREWATRATLTETDVVEERGVILDEWRTRAQGFAARVNEQIESLVLPGT
                     IYEGHQPIGNADSIETTSPQELARFYEDWYHPERMAIVAVGDFDAAAVEDLIEDSFGD
                     IPPADGARAWETPGFEAPTTPRAVSFVDEEATIAGVTVMWPTPNPPMQTVGDYQRSLA
                     TSFGMEILANRLSDDASAGDEALLGAAVVDLGWTRAFSIEGVDAEVRASQVDAGMVKV
                     LHEVERIRREGISEDEFQRQLAGYESFSRQIYEQRESAQDLQFTSQIVAHHLAGAHLM
                     SPGQRFDVESGILARITADDVDAALLAIVERSPVVLVVGPDDAGLDIPDETRILEVVA
                     GMPTAELGERRDVEPGEGELMDRPEPARIVSSTIDPRFEFTTLHFDNGATVYLWESDI
                     ASESVFGIIEGFGGTSQVEVEDLPEAFLMSEIVGRSGVAGFDVPTLRRLLAGAIVGVF
                     PWITETRQGLEVTASIEDVETMFQLIHLTMADPRFDDTAIDAVLNEMETLNASRDDVP
                     DLLFEEALNALYYGEDPRYFVIPSSGELAEFDVAVAERLFNERFGDAADFAFAFVGDF
                     DTADMTELAASYIGTLPGSGEPSSYVDHQPLPPREVQVRRVEAGIGEQGRIGMFFTNE
                     FEGDLLDRLHARLVELILTARLRERIREELSATYSIQAGIDLQRDPDRFAEAFITSTG
                     DPSGLDQIAEEILAGLQSLQDSGPTGEEFATAIEQLRDELELLDNRTLANALITSHLY
                     PDQPVAEVADRYQMVDSLTPEEVQLMARVAFDLDQRIEVRLVPRT"
     gene            5690..6667
                     /locus_tag="VFZ80_03635"
     CDS             5690..6667
                     /locus_tag="VFZ80_03635"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX6286553.1"
                     /translation="MTHILGFGGYAPSRVVTNDDWSKWVDTSDEWITQRTGIKERRFA
                     ADDESTMTLATQAAASALKHADLTPDDIDEIVLATDTPEMMTPDTAALVQNQLGCRNI
                     PTYDLGGSGCAGFVQALDVARSRILVEPKKVLVIGVELISRMISMKDRATAVLFGDGA
                     GAVVMGPDRGRAEVLGVVAGTDGSAAEILTLAAGGTRNPFNAESLANGDYNRLVMDGR
                     RVFTEAVKRMSEAASDVLAKVGRSLSDVKLVIPHQANIRIIDAVRKKMGLEESAVYVN
                     IDRFGNTGSATVPFAMWQAVNEGRIEEGDLVVLTAFGAGFHWAAAAVQF"
     gene            complement(6664..>7266)
                     /locus_tag="VFZ80_03640"
     CDS             complement(6664..>7266)
                     /locus_tag="VFZ80_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6286554.1"
                     /translation="PTFHEHGITSLVIDYRGDVGTPADPSGHYRFGLSEWEDVEAAAR
                     FALDRGATDLVMTGCSTGGALVMSFLERSDLASKVIGVVLDAPNIVLADTFRHSMRDL
                     RHSQLVKEMGMWIADLRWHIDWETTNYVQRADQFLRVPTLVFHGTSDARVPISSSRQL
                     QTALPALVELVETPAAGHVLSWNADPERYQRYLARFLASL"
BASE COUNT         1319 a         2291 c         2382 g         1274 t
ORIGIN      
        1 gtcaaccagg tgtacgaggg caagggcaac agctacttcc tgatcaacgg agtcaacgct
       61 gtcatcgcct atgccatcgt cggctacatc gtcaccgtct ggacctgagc gggagacgcc
      121 gaaccagaca tagatcactc atgcccgggt ctatcgcgat aaggccgatg aaaccttcag
      181 actggcccgg agtggcccgg gtctacgccg aggggatcag caccgggatg gccactttcg
      241 agaccagcgt tccaggttgg gatgattggg acgcgggcca tatcgaggca tgcaggcttc
      301 tggtggaggc cgacggggag atcgttgcct gggctgccct gagccccacc tcgcggcggg
      361 atgtctaccg aggcgtcgcc gaggcgagta tctacgtggg cgagaaggcg cgtggtaagg
      421 gaatcggctc tgcattgctc ggcgagctgg tgtcgtgcag tgagcgagag ggctactgga
      481 ccttgcaggc cggcatcttc cccgagaacg aggcgagtgt tcgtctccac cagcggcatg
      541 ggtttcgggt cgtcgggacc agggagcgaa tcggggccag caacgggagg tggcgcgatg
      601 tgctgcttct cgagcggcga agtgagacga tcgggcagta gccccccagg ccagcggggg
      661 aagcgccccg ttgcgggtcg cgtccgccgc tggccgcccc gtttcgggct cccgggccaa
      721 ggaggtccga taccgattcc cgaccaccca acgggtcggg aatcgtcatg ccgatgattg
      781 acccagacac cccccggcgc cttcgaccat ccgcacatcg gacctccgta cggcgtcctc
      841 gcggcgaaca ctatccgact tggttcacac tgtcccgagg tcgggaaaat taccgctatg
      901 gcacgccagg gctcgtcctc gacggcggct tggtgccgga tcaggcggga gtagctctct
      961 ccagcaaggt ctcgacgctc gtccctggtg ggagtgtccc gtacaagccc ccatgtctct
     1021 ccccgagacg tgacgacgcg aaggcctcga agatggctgg ttctccttgg cgagccagga
     1081 gagaacccgc ccaggcgaca gccatcgact cgacgatgcg gcgggcttgg aactcgatat
     1141 cagtcgctgt tgtcagcgcc gtcctggcgg cttcgatcgc gaagtcgaga cgggtgtcca
     1201 tgcccgaggc ctggtcgatc tcggtcagga acatctcgag actgtcggga gagcggccga
     1261 cggcgcggag gacatcgagg gcgatcacat tccccgagcc ttcccagatc gcattgagcg
     1321 gcgattcccg gtagagcctc ggcatgatcg actcctccac gtagccgttg ccaccgaggc
     1381 actcgagggc ttcgcgcacc acctcactgg ttcgcttggt gacccagtac ttggcgacgg
     1441 gcagagcgat ccgcttcatc gccgcctcgg acggatccac ctcggatcgg tcgaaggcgc
     1501 cggccaggcg aatcatcatc tcggtagccg cctcggtctc gacctcgagg tcggctacga
     1561 cgttgcgcat caacggcttg tcgatgagct tcgagccgaa tgcagcccgg tgtgagacgt
     1621 gccacgctgc ctgagagacg gcctgacgca tgagcgccgc cgagccgatg acacagtcga
     1681 gtctcgtgcc gttgaccatc tcgatgatcg tgggcacgcc acgtccctcg tcaccgacca
     1741 tgaccgccca agccgcgtcg aactccactt cggaggaggc attggagcgg ttgcccagct
     1801 tgtccttgag acgttgaatg tggaaggcgt tgagtcgacc gtccggcgcc caccgaggga
     1861 gcaggaagca cgagaggcca cccggcgcct gtgccaggac caggaaggcg tcgcacatcg
     1921 gcgccgaggt gaaccacttg tgcccggtga tggcatactc ggcacctgtc ccgccgccgt
     1981 tcagcggcac tgcctcggtc gtgttggccc ggacatcgga cccaccctgt ttctcggtca
     2041 tgcccatccc catgagtagg ccctgtttct ccttggcggg gcggaacgat gggtcgtagt
     2101 cgtgactgag gatccccgga atccactctc gcccgacctc cggtgtggtc tgcagggccg
     2161 ggatcaccga gtaggtcatc gagatcgggc agttgtggcc ggactcgatc tggcctgtca
     2221 ggtacgtgaa gacggcccgg gcgacatgag ccccttccac ggcttcctcc tcccagggaa
     2281 gactgtggtt gccgttggcg acggcggttt gcatcagcct gtgccacgag gggtggaact
     2341 cgactcgatc gacacggtgg ccgtggcggt cgtgcgtgat caactccggg tcgttgcggt
     2401 tggcatcgaa cccccaccgg tagtactcaa cagacccggt gagatccccg aactcgatca
     2461 gtcggtcacg ggaacccttg gcgccctccc ggcctaccgc ctcgtcgagc acgggatcgg
     2521 aggtgtagag gttgtagccc tctagtggtg gcacctggtt caggacctcg tgggtgtgac
     2581 tcacccaccc gaggataaga ccgttcgccg cggatctgtg atgcgcaaga ggtcggaaca
     2641 agcctgctca cggcagggag cgaatatccg cccgaccgtc atatggttcg gcaacgtgag
     2701 gagtcgatga gggaccgcat tcgagggtgc gccgtcggcg cagccctggt cgtcgtggtg
     2761 accgcctgca ccggctcagc cgaccccacc accacgaccg cccatgccgt acccaccacc
     2821 tcggtactgg cgagcaccac gacgaccgaa gacccgattc tgaatctcgc cctgcccgtc
     2881 gaccccgacg tggtccaggg tgttctcgac aacggcatga cctactacat cagggagaac
     2941 gactcccccg ggggtcgggc cgagctgaga ctcctcgtcg atgcggggtc ggtgcaggaa
     3001 gaccccgatc aggcgggaat ggcgcacttt ctcgagcaca tgatgttcaa cggcaccgag
     3061 aactacccac gtaacgactt gatcgacgtg ttagaggcgt tcgggccccg cttcggcccg
     3121 gatatcaacg cgttcacgtc atatgacgag accgtctacg agttgagcct gaccaccgac
     3181 aaccccgagc tcctcgtcct gggcgtcgat gtcctccgcg agtgggcgac gagggcgaca
     3241 ctgaccgaga ccgacgtggt cgaggaacgt ggcgtgatcc tcgacgagtg gcggaccagg
     3301 gcccagggct ttgcggcccg ggtcaacgag cagatcgaga gtctcgtcct gcccgggacg
     3361 atctacgagg gccaccagcc gatcgggaac gccgactcga tcgagaccac gtctcctcag
     3421 gagctcgcgc gcttctacga ggattggtac cacccggaga ggatggcaat cgtcgccgtc
     3481 ggcgacttcg acgcagcagc cgtcgaggac ctcatcgagg attctttcgg tgacattccg
     3541 ccggcggatg gcgcgcgagc ctgggagaca ccgggtttcg aagcacccac cactccccgg
     3601 gcggtctcct tcgtggacga ggaagcgacg atcgccggtg tgacggtgat gtggcccacc
     3661 cccaacccgc ccatgcagac ggttggcgac tatcagcggt cactggcgac gagtttcggg
     3721 atggagatac tcgccaaccg tctcagcgac gatgcgtcgg ctggcgacga agcgctactc
     3781 ggcgccgcgg tcgtcgacct cggatggacc agggcctttt cgatcgaggg cgtggacgcc
     3841 gaggtgcgtg cgagccaggt cgacgccggg atggtgaaag tccttcacga ggttgagcgg
     3901 atcagacgcg aaggcatctc cgaggatgag ttccagcgtc agctcgccgg ctatgagagc
     3961 ttctcacgtc agatctacga gcagcgagag agtgcacagg accttcagtt cacgtcgcag
     4021 atcgttgccc atcatcttgc cggggcgcat ctgatgagcc caggacagag gttcgatgtc
     4081 gagtccggga ttctcgcacg gatcacagcc gacgatgtcg atgcggccct gttggcaata
     4141 gtggaacgat ctccggtggt gttggttgtc gggcccgacg acgcgggctt ggacattccg
     4201 gatgagacac gcattctcga agtggtggcc ggcatgccca ccgccgagct cggggagcgc
     4261 cgggatgtcg agccgggcga gggcgagctc atggaccggc ccgagccggc gcggatcgtg
     4321 agctcgacga tcgaccctcg tttcgagttc acgacactcc atttcgacaa cggggcaacc
     4381 gtgtatctct gggagagcga catcgccagc gagtcggtgt tcgggatcat cgaggggttt
     4441 ggcggcacat cacaggtcga ggtggaagac ctgcccgagg cgtttctcat gtctgagatc
     4501 gtgggacgta gcggggtggc gggtttcgat gtcccgaccc tccggcgatt gctggcaggt
     4561 gccatcgtcg gcgtgttccc atggataacc gagacccgtc agggactgga ggtcaccgcc
     4621 tcgatcgagg acgtcgagac gatgttccag ctcatccatc tcacaatggc cgatccccga
     4681 ttcgacgaca ccgccatcga cgcggttctc aacgagatgg agaccctgaa cgcctcacgt
     4741 gacgatgtcc ccgatctctt gttcgaggag gcgctcaacg ccctctatta cggggaggac
     4801 ccccgctatt tcgtgatccc ctcgtcgggg gaactggccg agttcgatgt tgcagtggcg
     4861 gagcgactgt tcaatgagcg cttcggagac gctgccgact tcgcctttgc gttcgtgggc
     4921 gacttcgaca ccgcagacat gaccgagctg gcagccagct acataggcac tctgcccggg
     4981 tccggagagc cctccagcta cgtcgaccat cagccattgc caccgcgcga ggtccaggtg
     5041 aggagggtcg aggccgggat tggcgaacag ggccggatcg gcatgttctt cacgaacgag
     5101 ttcgagggag acctcttgga ccgccttcac gcccgactgg tggagctgat cctgaccgcc
     5161 cgtctccgcg agcgcatacg cgaagagctc tcggcgacct actcgatcca ggcgggaatc
     5221 gacctgcaac gagatcccga ccgtttcgcc gaggccttca tcacctcgac aggggatcca
     5281 agcggattgg accagatcgc cgaagagatc ctggccggcc tccaatccct gcaggacagc
     5341 ggcccgaccg gggaggagtt cgccaccgcg atcgagcagc tgcgcgacga gctcgagctg
     5401 ttggacaaca gaaccctcgc caacgccctc atcaccagcc acctctaccc cgaccagccg
     5461 gttgccgagg tggccgatcg ctatcagatg gtcgacagcc tcacgccgga ggaggtgcag
     5521 ttgatggctc gtgttgcctt cgatctcgac cagcgcatcg aggtccggct ggtgccccga
     5581 acttgagcgg tccgaagcgt tgttgttgca tcctgtctca gaaaccacgt catcctgctg
     5641 tggcaacgcc aggactgcgc gaccggaccc gccgccgtag tgtgagggca tgactcacat
     5701 cctcgggttc ggcggttacg ccccgtcgcg cgtcgtcacc aacgacgact ggtcgaagtg
     5761 ggtggacacg tccgacgagt ggatcaccca gcgcaccggg atcaaagagc gtcggtttgc
     5821 cgcagacgac gaatcgacca tgaccctggc gacccaggcg gcagcttccg ccttgaagca
     5881 cgccgacctg accccggacg acatcgacga gatcgtcctc gccaccgaca cgcccgagat
     5941 gatgaccccg gacacggcgg ctctggtcca gaaccagttg ggctgtcgca acatccctac
     6001 ctatgacctc ggtggcagcg ggtgcgccgg gttcgtccag gccttggatg tagcccgttc
     6061 caggatcctc gtcgagccca agaaagtcct ggtcatcggg gtcgagctca tctccaggat
     6121 gatctcgatg aaggaccggg ccactgccgt cctcttcggc gatggagccg gtgcggtggt
     6181 gatgggcccc gaccggggga gagccgaggt cctgggtgtg gtggcgggaa cagacggaag
     6241 tgcggcggag attctcacac tggccgccgg cggtacccgc aaccccttca acgccgaatc
     6301 gttggcgaac ggtgactaca accgactggt gatggatggc cgaagggtct tcaccgaggc
     6361 cgtgaagcgg atgtcggagg cagcctcgga tgtgctcgcc aaggtgggtc ggtcgttgtc
     6421 agatgtgaag ctggtgatcc cgcatcaggc caacatccgg atcatcgatg cggtgcgcaa
     6481 gaagatgggt ctcgaggaga gcgcggtcta cgtcaacatc gaccggttcg ggaacaccgg
     6541 ctcggcgacc gtccctttcg ccatgtggca ggcggtgaac gaaggtcgca tcgaagaggg
     6601 cgatctcgtc gttctgaccg cattcggggc cggcttccac tgggctgcgg ccgccgtcca
     6661 gttctagaga gacgccagga acctcgccag atagcgctgg taacgctccg ggtcggcgtt
     6721 ccaactgagg acgtgaccgg ccgccggggt ctcgaccagt tcgacgagcg ccggcaaagc
     6781 cgtctgcaac tgccgactcg acgagatcgg caccctggcg tccgaggtgc cgtggaagac
     6841 gagagtcggc acacggagga attggtcggc ccgctgaacg taattggtgg tctcccagtc
     6901 gatgtgccat cgcaagtcgg caatccacat ccccatctcc ttgaccaact gtgagtgccg
     6961 caggtctcgc atggagtggc ggaaggtgtc ggcgagaacg atgttcgggg catccaggac
     7021 cacgccgatg accttgctcg ccaggtcgga ccgctccagg aatgacatca ccaatgcgcc
     7081 gccggtgctg cacccggtca tgacgaggtc tgtcgctccc cggtccagcg cgaaacgggc
     7141 cgcggcctcg acgtcttccc actcgctgag cccgaatcga taatgcccgg atgggtctgc
     7201 gggtgttccc acgtcgcctc ggtagtcgat gaccagcgag gtgatcccgt gctcgtggaa
     7261 ggtggg
//
LOCUS       DASUTA010000183         2420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_185340, whole genome shotgun sequence.
ACCESSION   DASUTA010000183 DASUTA010000000
VERSION     DASUTA010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185340"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VFZ80_03645"
     CDS             <1..256
                     /locus_tag="VFZ80_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286555.1"
                     /translation="RGAAWLAKRVGVPIVPVAVIGTGLAFGLENRLRRAPVRVVLGRA
                     IDSEIDDAEGLTRLWAGWISAQIARHADSETSGPPRAFAT"
     gene            complement(191..826)
                     /locus_tag="VFZ80_03650"
     CDS             complement(191..826)
                     /locus_tag="VFZ80_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286556.1"
                     /translation="MLFGLVLIASGIGHVVNDPADNESAEFGELVESLIDRQSDQVMD
                     TEGLASRLGYVPAVESGAWVDPDGICSTPIPVGPADFDGPCRSHDLGYDTLRLAASDD
                     MGLGAWARLGLDARLYTDLLETCDDDVGCRAKATLYFGAVTANSIRQGYRSPPAEPVL
                     PWAGLGVTVVGLSLFPRRAESPVRDRRLQSSGRKRPWGARSLGVGVPGNLS"
     gene            complement(1066..1713)
                     /locus_tag="VFZ80_03655"
     CDS             complement(1066..1713)
                     /locus_tag="VFZ80_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6286557.1"
                     /translation="MTGTLRPRVFVVEDHPLMLSGIQRALEERYDLVGSASEVAAAVE
                     MILDRRPDLVLLDVRFEGGGGSAVIEGVKQRDDDIKFLAYTVSTSAEDVVRMFRVGVD
                     GYVVKKADGDELAENIDQVLAGGRPVSRFVANYLMQLDDVVATSPELEGLTPKEREVM
                     TLIARGYRYREVAEELGISVKTLETHMKHIFDKLGVASRHEVTRIAFATGFVDPD"
     gene            complement(1710..>2420)
                     /locus_tag="VFZ80_03660"
     CDS             complement(1710..>2420)
                     /locus_tag="VFZ80_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6286558.1"
                     /translation="LSPVRIVGSVQFLIVALIVSWAFDTLRSEERLRLTAQLEAREAQ
                     RALLAEQGVAARLEERSRIATRLHDSVLQTLKLIMSNANDANEVRYLARIQERELRRT
                     INQYRSPHADGLRARLLDARADVEDRHRVEVEQVIKGDTEMTPRLEGLVAAVHEAMTN
                     AARHAGDSTIDLFVEIRDEGIQVNVRDRGPGFDAVSRLGSGIQHSIVEPLQRVGGHVN
                     VKSTVGAGTDISIYLPAE"
BASE COUNT          447 a          790 c          749 g          434 t
ORIGIN      
        1 ccgtggcgcg gcatggctcg ccaaacgggt tggtgttccg attgtccccg tggcagtgat
       61 cgggaccggc ctggcgttcg gcctggagaa ccggctgcga cgggcgccag ttcgggtggt
      121 gctcggccgc gccatcgact cagagatcga tgacgctgag ggtctcactc ggctttgggc
      181 gggatggatc tcagctcaga ttgccaggca cgccgactcc gagacttcgg gccccccaag
      241 ggcgtttgcg acctgaactc tggagacgcc gatcccgaac aggagactcg gcacggcgcg
      301 ggaacagcga caacccgacc acggtaacgc caagacccgc ccaaggcagt acgggctcgg
      361 cgggtgggct gcgatatccc tgtctgatcg aattggcagt gaccgcaccg aagtagagcg
      421 tggccttggc ccgacaaccg acgtcgtcgt cgcaggtctc gagaagatcg gtgtagagcc
      481 gagcgtcgag cccgagccgc gcccacgcac ccaagcccat gtcatcggag gctgcgagcc
      541 tcagggtgtc gtaaccgaga tcgtggctcc gacaaggccc atcgaaatca gcgggaccga
      601 ccgggatcgg cgtcgagcag atgccatccg gatccaccca agcaccggac tccacggcgg
      661 gaacataacc caacctcgag gcgagcccct cagtgtccat gacctggtcg ctttgtctgt
      721 cgatcaacga ctcgaccagt tcgccgaatt cggccgactc gttgtcggcg ggatcgttga
      781 cgacgtgacc gatgccactg gcgatcagca ccaatccgaa tagcactgag aggcggctgc
      841 cgcagccgag cctccagaag cctcggcaga ctgccgtcga catgatccgt tgcgtcacct
      901 tcgtcgcgcc ggtggccaac gacgacgtca ccaatccccg ggtcccgatc gaaatgcagg
      961 tgatctgtct cacagcccca cgatgccggg ccgctcggcc ggcgactatc ggggtcctcc
     1021 ctcacggggg atttccctag gggtttgccc tgatcccgcc gcggttcaat ccggatcgac
     1081 aaaccccgtc gcaaaggcga ttcgggtcac ctcgtggcgg ctggccaccc cgagtttgtc
     1141 gaagatgtgc ttcatgtggg tctcgagcgt cttgacactg attccgagct cctcggccac
     1201 ttccctgtac cgatagcccc gggcgatcaa cgtcatgacc tcccgctcct tcggagtcaa
     1261 gccctcgagc tcagggctcg ttgccacaac gtcgtcgagc tgcatcaggt agttggccac
     1321 gaagcgagac acgggacgcc caccggccaa cacctgatct atgttctcgg ccagctcatc
     1381 gccatctgcc ttcttcacca cgtatccgtc gacgcccacc cggaacatcc gcaccacgtc
     1441 ctctgcggaa gtggaaacgg tgtatgcgag gaacttgatg tcgtcgtcgc gctgcttgac
     1501 tccttcgatg acggctgagc cgccgccgcc ttcgaatctc acatccaaga gaaccaggtc
     1561 gggccgacgg tcgaggatca tctccaccgc agccgcgacc tcagaggcag aacccaccag
     1621 gtcgtaacgc tcctccaggg ccctttggat gccggagagc atgagcggat gatcctccac
     1681 cacaaagacg cgaggacgca gggtgccggt cattccgcag gcaggtagat cgagatgtcg
     1741 gtcccggcac cgaccgtgga tttgacgttc acgtgaccgc cgacgcgctg caggggctcg
     1801 actatggagt gctggatacc tgatccgagc cgggatacgg catcgaagcc cggtcccctg
     1861 tcgcgcacat tcacctggat accctcgtcg cgaatctcca cgaagaggtc gatggtcgag
     1921 tctccggcat gtcgcgctgc attggtcatc gcttcatgaa ctgcagcgac caggccctcc
     1981 aggcgcggag tcatctcagt gtcacccttg atcacctgct ctacctcgac ccggtgccgg
     2041 tcttcgacat cggcgcgagc gtcgaggagg cgggcacgaa gaccgtcggc atgaggcgag
     2101 cggtactggt tgatggtgcg acgtagctcg cgttcttgga tccgggccag gtaccggacc
     2161 tcgttggcgt cgttggcgtt ggacatgatg agcttgagcg tctgcaggac cgagtcgtgg
     2221 agacgcgttg cgatcctcga tcgctcttcg agtcgtgctg ccaccccctg ttcagcgagc
     2281 agggctcgtt gtgcctcccg ggcctcgagc tgagccgtga gacgcagtcg ctcctcggaa
     2341 cgcaaggtgt cgaaggccca actcacgatg agtgcgacga tcaggaattg gacgcttccg
     2401 actatgcgga caggtgaaag
//
LOCUS       DASUTA010000184         2258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1092800, whole genome shotgun sequence.
ACCESSION   DASUTA010000184 DASUTA010000000
VERSION     DASUTA010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092800"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..641)
                     /locus_tag="VFZ80_03665"
     CDS             complement(240..641)
                     /locus_tag="VFZ80_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286559.1"
                     /translation="MTDRHPGHEDHSHESKHSHEGEHSHDQDHDTNSSEAHTRAEDRT
                     HDQEHDREEPSNKWVKDAEDALAGAGEAIRTAWDASRDARMTALEAARNAVEALGDAI
                     EQGLSAARTRKQETTETEPAAPDTPRTDDEE"
     gene            781..1095
                     /locus_tag="VFZ80_03670"
     CDS             781..1095
                     /locus_tag="VFZ80_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX6286560.1"
                     /translation="MADETQLMVWIDQDLCTGDGICSEICPDVFEPRDDGLWVVKEEA
                     NNFGRRVIFDGLGNDDSGPDGARGLARIPDDQIDLAIEAAEECPGECIMIQPFQADLV
                     HS"
     gene            1137..2045
                     /locus_tag="VFZ80_03675"
     CDS             1137..2045
                     /locus_tag="VFZ80_03675"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX6286561.1"
                     /translation="MNRPRRVEITESSPRDGLQSLGVFIPTAAKIGLIDDLALAGLKS
                     FDAVSFVSPTAVPQMADGRDVVEGVARNGIRLNGLVPNLKGLEAAVEAGVDGIGVLAA
                     ASDTFSLRNINATVDEAMDRIRGILTEAPTDVATRAYVSTVTHCPYEGPTDSDRVAHL
                     VETILEWGCDDVFLGETLGKGTVADVERVLGKVLAVVPVEPIGVHFHDTYGQALANVV
                     ASLDAGINRLDSSAGGLGGCPYAPGAAGNVATEDVMWLLDGMGIDYDGELAAVAATAN
                     RFCSDWKLRYRSRAGAAVLAGNGGDE"
     gene            2042..>2258
                     /locus_tag="VFZ80_03680"
     CDS             2042..>2258
                     /locus_tag="VFZ80_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6286562.1"
                     /translation="MNIYLNEDLRAISGQIAEYVEREIVPSGEEWEQAGRVPRDVLRR
                     MGEIGFFGLRVEEEYGGIGLGPLTSVVF"
BASE COUNT          370 a          679 c          788 g          421 t
ORIGIN      
        1 ggtcggcggc gacgacttcg gtggggggat gcgggtagag cagtggcgcg acctcaacga
       61 cgccatcgat tcgggttcgg gactggccga gctccagcga cggatcagcg acctgaagga
      121 gatactctcg taatggacca gttcaaggag cgacagccct cataggccga gtgccggtga
      181 tcgaccccct tcaaggaacc ggcttggctt ccgacttccg acttccgact gaggcgcgct
      241 cactcctcgt cgtcggtccg gggagtgtcc ggggcggctg gttcggtctc cgtggtttcc
      301 tgcttgcggg tccgtgcggc gctgaggccc tgctcgatcg catcgcccag cgcctcgacc
      361 gcgttacgag ccgcctccag ggcggtcatc cgggcgtcac ggctggcgtc ccaggcggtg
      421 cggatcgcct cgcccgctcc ggccagggca tcctccgcgt ccttgaccca cttgttcgac
      481 ggctcctcgc gatcatgctc ctgatcatga gtgcggtcct cggcccgggt gtgggcctcg
      541 gacgagttgg tgtcgtggtc ctggtcgtga gagtgctcgc cttcgtggga gtgcttgctc
      601 tcgtgcgagt ggtcttcgtg acctggatgg cggtcggtca tggtcgcgcc tttcgggttc
      661 gcctgcttga tggaatcatc gcgcacttcg aggtcctatg acggtcgagg aggcgaggac
      721 gcttcggatt gatacccgga gcgaatatgg ttggatacag cgaaccaacg agaggattca
      781 atggccgacg aaacccagct catggtctgg atcgaccagg atctttgcac tggggacggg
      841 atctgttccg agatctgtcc cgatgttttc gagccgcgtg acgacggtct ctgggtggtg
      901 aaggaagagg cgaacaactt cggtcgccgg gtgatattcg atggcctcgg caacgatgat
      961 tccggacccg atggcgcccg cggtctggcc cgcatccccg atgaccagat cgacctcgcc
     1021 atcgaggccg ccgaggagtg ccccggtgag tgcatcatga tccagccttt ccaggctgat
     1081 ctcgtccaca gctaggccga atcgagccct tctcgagcgt cggtatcctg cccggtatga
     1141 accgtccccg ccgggtcgag ataaccgaat cgagcccacg cgacggtctc cagtcgctgg
     1201 gagtcttcat accgaccgca gccaagatcg gtctcatcga cgatctcgcg ctggccgggt
     1261 tgaagagctt cgacgccgtc agcttcgtca gtcccaccgc ggtgccgcag atggccgacg
     1321 gcagggacgt cgtcgaaggc gtggccagga acgggatacg cctgaatggt ctcgtgccga
     1381 acctcaaggg tctggaagcg gcagtcgagg cgggcgtcga cgggatcggc gtgctggcgg
     1441 ccgcctccga cacgttcagc ctgcgcaaca tcaacgccac cgtcgacgag gccatggaca
     1501 ggatccgcgg gatcctgacc gaagctccca ccgatgtggc cacgagagcc tacgtctcga
     1561 ccgtcaccca ttgcccctat gagggaccga ccgattccga ccgggtcgcc catctcgtcg
     1621 agaccatcct cgagtggggt tgcgacgatg tcttcctggg agagaccctg ggaaagggca
     1681 ccgtcgccga cgtcgagcgg gtgctcggca aggttctggc ggtggttccg gtcgaaccga
     1741 tcggtgtcca cttccacgac acctacgggc aggcgctggc gaacgtggtg gcctccctcg
     1801 atgcgggtat caaccgtctc gattcgtcgg cgggtggact cgggggatgt ccctacgcac
     1861 cgggagcggc cgggaacgtg gctaccgaag acgtaatgtg gctgttggac ggcatgggga
     1921 tcgactacga cggagagttg gcggcggtcg ccgcgacggc gaaccgcttc tgttcggact
     1981 ggaagctgcg ttaccggagc cgggcgggag cagccgtgct ggcgggtaac gggggagacg
     2041 aatgaacatc tacctgaacg aggatctccg ggccatctcg ggtcagatcg ccgagtacgt
     2101 cgagcgggag atcgtgccta gcggggagga gtgggaacag gcgggcaggg taccgagaga
     2161 cgtcctccgc cggatggggg agatcggttt cttcggcctg cgagtggagg aggagtacgg
     2221 ggggatcggg ctgggtccgc ttacctcggt cgtattcg
//
LOCUS       DASUTA010000185         7531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_443733, whole genome shotgun sequence.
ACCESSION   DASUTA010000185 DASUTA010000000
VERSION     DASUTA010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7531
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443733"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /locus_tag="VFZ80_03685"
     CDS             complement(<1..1514)
                     /locus_tag="VFZ80_03685"
                     /inference="COORDINATES: protein motif:HMM:NF021588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-hydrolase family protein"
                     /protein_id="HEX6286563.1"
                     /translation="MTQNDLNLFARAGIAMATRVNPVTGGPAALLKANALLDAYGPSL
                     MPRKSLHQGMAGGLAILAAELVGRGVDAVVKKAAPDSSPLSWRLGARAGLTGLGAVIA
                     RISASEDEPTSRAALRSAGRLVEVAALGGIVNEAGRDLEKKVDWPLLPVLTGLGGFGY
                     VATRLAKELETREGMIQRWTEDDTPASLPASMGIGVAVAVAGRMIGRGYMTSRDSLGD
                     YVGGDRDGPKLVGRLINAGMWAGGATALYGAVVARLARLNGKVETNFSTPPSNQFVSG
                     GPNSISPFEELGLQGRRFVTEAVPPQEIAETLGESGAVHPIRVYVGVESEPLYATGRS
                     EIALREMERLGAFDRKYLLLVSPTGTGWVDHTVAETAEILARGDIATVCIQYGKAPSF
                     LEIQGVSLGRAQFRQLLWGVNQRLTGVPQDERPKVLVFGESLGAWSSSDVIMHQGIAG
                     FDHYGIENALWFGLPGLARWSRTGMREGRNPLTPVGTVGAFYRFEKYQELPAKA"
     gene            complement(1549..2397)
                     /locus_tag="VFZ80_03690"
     CDS             complement(1549..2397)
                     /locus_tag="VFZ80_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX6286564.1"
                     /translation="MARWERLGTRGNVTDRRGLGGPGMAIGGLGGVGLIVFLLISLLG
                     GGSGLEDVLGQLATPAQPVEQSADFEGLDEYELFTRRVLGSTDKTWSAIFEGSGLTYP
                     IPELILFRGATSSACGGASSQVGPHYCPLDETIYLDETFFDELTNRFGAEGGDVAEAY
                     VIAHEVGHHVQKRLGIMEQVQSLQQQASSQEEANDLSVRLELQADCFAGIWANSIRDE
                     GVFLPGEINEALDAAAAVGDDRIQQAVTGQVSPERWTHGSSEQRVEWFTVGFDTGDPS
                     ACDTFG"
     gene            complement(2399..2776)
                     /locus_tag="VFZ80_03695"
     CDS             complement(2399..2776)
                     /locus_tag="VFZ80_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286565.1"
                     /translation="MSNYPRSVLFRLAGAFAGAALWLGLIVLALTNFDTPAGLPEELQ
                     ATWPFWLVAIVITVGVFLVRRRLEPGPGWGMFAIGVVAPFVGLVLNANMGGGGGLWFW
                     MPVLFLVFVPLPRFWAKQRAGEI"
     gene            complement(2807..3496)
                     /locus_tag="VFZ80_03700"
     CDS             complement(2807..3496)
                     /locus_tag="VFZ80_03700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX6286566.1"
                     /translation="MTLAAAVAPLAAYAVVFQTLLYLSLPQRLRTRLAPVVTGVVGAG
                     LSIAAGLIFGFDTVGLVGGELVVTLAWGAVTVAGMAVLGLAMLRRPDLRMQLADPRLA
                     DLSRSEAFSQIFVRIPVFTALIEEAFFRGVLHAALMALYPVEVAVWLGAGLFGVWHIG
                     PGVDQARAGEKSVRASAAHTALTVVATTVAGAFLVWLRIETGSIWAGVAVHAAVNMTL
                     AIFARAAGRVG"
     gene            complement(3589..3744)
                     /locus_tag="VFZ80_03705"
     CDS             complement(3589..3744)
                     /locus_tag="VFZ80_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018961044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286567.1"
                     /translation="MIILGVILLIIGALLDIGILYTIGGILAVIGVVLWLLGAFGRQV
                     GPRAHYY"
     gene            complement(3867..4079)
                     /locus_tag="VFZ80_03710"
     CDS             complement(3867..4079)
                     /locus_tag="VFZ80_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286568.1"
                     /translation="MMHPIDTTTIREHEADYPSSSEIRTGWWAIVALMILVALLVTVV
                     LVFSGDESSSDPEAPQPTEAPLDSGA"
     gene            complement(4193..4627)
                     /locus_tag="VFZ80_03715"
     CDS             complement(4193..4627)
                     /locus_tag="VFZ80_03715"
                     /inference="COORDINATES: protein motif:HMM:NF016102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEX6286569.1"
                     /translation="MRFVAVFGLIALAEMATFFWVGSQIGLAWALGLAVATAIIGSIL
                     VRRAGISVIQRIQERTRQGQLPGRELSDGAAVLVAGAFLISPGFITDMLGFLLLVPGV
                     RSLVYRLVSSRLSRRITVFGTGTSTGRWVEASGEVIDVEPED"
     gene            4684..6660
                     /locus_tag="VFZ80_03720"
     CDS             4684..6660
                     /locus_tag="VFZ80_03720"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX6286570.1"
                     /translation="MTLTNLSDKYWRKINELAPTIATVRGVHDYDTELPSFEEDRLEA
                     LASSFRQIRENAEALDESAMSTQEKITRSLLVHQCQSALDTIETPFLLASIDPFTGPH
                     TRLLSDTRQNTVSDASQAEDLLERYAKVPGFLEGAFRVQRRCSQEGMTPARAALDRVI
                     SQLDGYLDSGLEDDPFLVLSVPGDGESWRERAEKVVTETIRPAVQEYRDSLIHYLAPT
                     ARPDDRVGLSWIPYGDEIYARLIGKYTQLETSASAIHDIGQEWATGINAGEWVEIGSK
                     AFGLDSLEAVFERLHSDPDLRFSSEEHMLEHARVALDRAWKEVDAWFAHRPATPCTVV
                     PVPAAVAPAMPPAYYSQPPVDGSRPGTYFLNTYKPEERDLFEYESIHFHEGIPGHHFD
                     RSLAAELEGIPTFRRYAMVYAHTEGWGLYSERLADEMGLYSNETDRLGMVSADAWRAG
                     RLVTDTGMHALGWSRQQAIDYLMRWTPISRLTVEQEVDRYIGMPGQALAYKMGQLEIL
                     RLRDEARDRLGSSFDIKGFHDTMLVNGAMTLPMLSTAVDEWMGRSPDVSPGGPSGYSL
                     DATTEGEDMAKDHGPSIKDDERYEELREEGMSKEKAARIANTPQEEASKKGGQSPPYE
                     DWTVDDLRDRAAEIGIEGRSDMDKDELIDALRNH"
     gene            complement(6665..7159)
                     /locus_tag="VFZ80_03725"
     CDS             complement(6665..7159)
                     /locus_tag="VFZ80_03725"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HEX6286571.1"
                     /translation="MTHRLKNGEPEPKDQEEIDDQFRALMEGLRTTIPGVMVLFSFLL
                     ILPLQASFADLGGINKAVFYVAFGSAALASVLLISPSVHQRVRAPISGIKRRTWSHIM
                     YATKMAIVGTVAFLVAISAVVYLVSSLVFSDPVAVVATVVLATIAGWAWFYVPLVKFD
                     SSDE"
     gene            complement(7296..>7531)
                     /locus_tag="VFZ80_03730"
     CDS             complement(7296..>7531)
                     /locus_tag="VFZ80_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286572.1"
                     /translation="GILPPSSSRQRWRSSQTGLRCGLPLWERSLSAGQLSQLAESCPQ
                     AMFFVPQRSPFVECVLREPTASFLDLAGASSFG"
BASE COUNT         1565 a         2422 c         2328 g         1216 t
ORIGIN      
        1 tacgccttgg cggggagctc ctgatatttc tcgaaccgat agaaggcgcc caccgtcccc
       61 accggtgtca gcgggttacg cccttcgcgc atcccggtgc gcgaccaacg agcgagacca
      121 ggcaatccaa accagagggc gttctcgatg ccgtaatggt cgaagccggc gatgccctga
      181 tgcatgatga catcggagct cgaccaggct cccaggctct caccgaagac gaggaccttg
      241 ggacgctcgt cttgtggaac tccggtcaga cgctggttga caccccagag gagctgtcga
      301 aactgggccc gaccgagaga gacaccctgg atctcgagga atgaaggtgc cttgccgtac
      361 tgaatgcata cggtggcgat gtctccccgg gccaggatct ccgccgtctc cgcaactgtg
      421 tgatcgaccc atcctgtccc ggtgggcgac accaggagca gatatttgcg gtcgaaggcc
      481 ccgagacgct ccatctcccg aagtgcgatc tccgaccttc cggtcgcata gagcggttct
      541 gactccactc cgacataaac ccggatgggg tgaaccgcac cactctctcc cagggtctca
      601 gcgatctctt gtgggggcac ggcttcggtg acgaatcgac ggccctggag accgagctcc
      661 tcgaacggtg agatgctatt gggcccaccc gacacgaact ggttgctcgg tggagtgctg
      721 aagttggtct ccacctttcc attgagtcgc gccagacgag ccacgaccgc tccatagaga
      781 gccgtggcgc ccccagccca catcccggcg ttgatgagac gaccgaccag cttggggcca
      841 tctctgtctc cacccacgta atcgccgagc gaatcgcggg aagtcatgta gcccctgccg
      901 atcatccgcc ctgccacggc cactgccacg ccgatgccca tcgaagccgg caaagacgcg
      961 ggggtgtcgt cctcggtcca gcgctggatc atgccctcac gggtctccag ctccttcgcc
     1021 aaacgggtgg cgacataccc aaagccgcca agaccggtga gcaccgggag caagggccaa
     1081 tcgaccttct tctccaaatc cctgcccgcc tcgttgacga tcccgccgag agcagccact
     1141 tcgaccagac gccccgccga ccgcagtgcg gcacgggagg tcggctcgtc ctcactcgcc
     1201 gagatcctgg cgatgaccgc tcccaggccg gtaagaccgg cgcgggctcc gagccgccac
     1261 gacagcggtg aagaatcggg agctgctttc ttgactaccg catcgacacc tcggcccacc
     1321 aattcggcgg cgagaatggc gagaccaccg gccattccct gatggaggga tttcctgggc
     1381 atcagtgaag ggccgtacgc atcgagcaac gcgttcgcct tgagcagtgc ggcaggcccg
     1441 cccgtcaccg ggttcaccct ggtcgccatg gcgatgccgg cacgggcgaa caggttgaga
     1501 tcgttctgtg tcacaccgag aagttagccg cggtgtagga ggcggtcgtc agccgaaggt
     1561 gtcacaggcg ctggggtcac ccgtgtcgaa tccgacagtg aaccactcga cgcgctgctc
     1621 cgaagagccg tgagtccatc gctccgggct cacctgcccg gtcacggctt gctggatgcg
     1681 atcatccccg actgcggccg ccgcatcgag cgcctcgttg atctcgccgg ggaggaacac
     1741 cccctcgtct ctgatcgagt tggcccagat gccggcgaaa cagtccgcct gcagttcgag
     1801 acgcaccgaa aggtcgttgg cctcctcctg agacgaagcc tgttgctgta aggattggac
     1861 ctgttccatg atcccgaggc gcttctggac gtgatggccc acctcgtgag cgatcacata
     1921 ggcctcggcg acgtcgccgc cctctgcccc gaagcgattg gtcaattcgt cgaagaacgt
     1981 ctcgtcgagg tagatggtct cgtccaacgg gcagtagtgg gggccaacct gggagctggc
     2041 gccaccacaa gccgagctcg tcgcgccccg gaagaggatg agctcgggta tcggataggt
     2101 gagaccggag ccctcgaaga tggcgctcca ggtcttgtcg gtcgaaccga gcacccgtct
     2161 cgtgaacaac tcgtactcgt ccagcccctc gaagtctgcg gactgctcta cgggttgtgc
     2221 gggagtggcc agctgcccga gcacatcctc gaggcccgag ccgccaccca gcagggagat
     2281 gaggaggaag acgatcagac cgaccccgcc gagcccgccg atggccatgc ccgggccacc
     2341 caggccacgg cgatcggtta cgttgccccg cgtgcccaac ctctcccagc gtgccatgtc
     2401 agatctctcc agcccgttgc ttggcccaga aacgaggcag tggcacgaag accaagaaca
     2461 gcaccggcat ccagaaccag agccctccac ctccacccat gttggcgttg aggaccaatc
     2521 ccacgaaggg ggcgaccacc ccaatggcga acattcccca ccctggtcct ggttctagcc
     2581 ggcggcggac gaggaagaca cccacggtga tcacgatggc caccaaccag aagggccatg
     2641 tcgcctgtag ttcctcaggg agtccggccg gggtatcgaa gttggtcagc gccaagacga
     2701 tcagacccag ccatagggcg gcaccggcga aggcgcccgc gagacggaaa aggacactgc
     2761 gtggatagtt ggacacgggc cgaggatatc cacggggtct attggttcag ccgacacgac
     2821 cggcagcccg ggcaaagatg gccaacgtca tgttgactgc ggcgtgaacg gcaacaccgg
     2881 cccagataga tccggtctcg attctcagcc agacgaggaa cgctccggca acggtggtgg
     2941 caacgaccgt cagcgcagtg tgagcagcgc tggcgcgaac cgacttctcg ccggctcgtg
     3001 cttgatcgac tccaggaccg atatgccaga cgccgaacaa tcccgcaccg agccagaccg
     3061 ccacctcgac cgggtagagg gccatgagag cggcatggag gacaccgcga aagaaggcct
     3121 cctcgatgag ggcggtgaat acgggaatcc tgacgaatat ctggctgaaa gcctcggaac
     3181 ggctgaggtc ggccagccgg gggtcggcga gctgcatacg gagatcgggt ctgcgcagca
     3241 tggccaatcc gaggacggcc atgcccgcca cggtgacagc tccccacgcc aaggtgacca
     3301 ccagctcgcc accgacgagc ccgacggtgt cgaaaccgaa gatgagtccg gctgcgatgg
     3361 aaaggcccgc cccgaccaca cccgtcacca caggggcaag gcgggtgcga agacgttgcg
     3421 gaagagacag atagaggagg gtctggaaca caaccgcgta tgcggcgaga ggggcgacag
     3481 cagctgcgag agtcatagat cgggggttag aactcgagaa tacaaggggt ccatgattga
     3541 gacgatccca ccgcccggat ggttcccgaa cggtgggatc tgcttcgctc agtagtagtg
     3601 cgctctcggt ccgacctggc ggccgaacgc tcccagcaac cagagcacga caccgatgac
     3661 agcaaggatg ccgccgatgg tgtagaggat gccgatgtcg agcaaggcgc cgattatcag
     3721 cagtatcact ccaagaatga tcatttttca ctgtcctttc actcagaccg gcgccccgta
     3781 cccacatcag acgttggaga aacccgggcc ggtctccgga cagcatgagg acccccgcct
     3841 gggcttcagc gggggccctc atgacttcat gcgcctgaat cgagcggtgc ctcggtcggt
     3901 tgcggtgcct ccggatcgga ggagctctcg tcgcctgaga agacgagcac cacggtcacg
     3961 agcagcgcca cgaggatcat cagcgccacg atcgcccacc agccggtgcg tatctcgctg
     4021 gagctggggt agtcggcctc atgctcccgg atggtggttg tatcgattgg gtgcatcatt
     4081 ccgcacctcc tcggtgagga agcccgcgaa taccccgggg gtcatcgggg caaacctctg
     4141 gtggcgcgtc ggccgggtgc gtcgagaggc cgcgaatcag agccggcggg cctcaatcct
     4201 cgggttcgac gtcgatcacc tcgccggagg cttccaccca tcggcccgtc gacgtgccgg
     4261 tgccaaacac cgtgattcgt cgggagagac gggacgagac cagacggtat accagggagc
     4321 gaacacccgg aacgagcagg agaaagccga gcatgtcggt gatgaacccc ggggagatga
     4381 gaaacgcccc ggcgacgagt accgcagcac cgtcgctcag ctcacggccg ggcaactgac
     4441 cctgcctggt cctctcctgg atccgctgga tcacagagat cccggcgcga cgcaccagga
     4501 tcgacccgat tatggcggtg gccaccgcaa gaccgagagc ccacgccaac ccgatctgtg
     4561 acccgaccca gaagaacgtc gccatctctg ccaaagcgat caaaccgaag accgcaacga
     4621 acctcatgga gcaacggtac agccgtgtga tctgaaccag acctctcggt actgtccctg
     4681 ttgatgactc tcacaaacct ctcggacaag tattggcgga agatcaacga gttggcgccg
     4741 accatcgcca cggtgcgcgg cgtccacgac tacgacaccg agttgccctc cttcgaggaa
     4801 gaccggctcg aagctctggc gtcgtccttt cgccagatcc gagaaaacgc cgaggctcta
     4861 gacgagtcgg caatgtccac ccaggagaag atcacccgct cgttgctcgt gcaccaatgc
     4921 cagagcgccc tcgacaccat cgaaacacca ttcctgttgg cgtcgatcga tcctttcacc
     4981 ggtcctcaca ccaggctgct ctcggacacc agacagaaca ctgtttcgga cgcgagccag
     5041 gccgaggatc tcctcgagag atacgccaag gtgcccggct tcttggaagg tgcattccgg
     5101 gtccagcgac gctgctcaca ggaaggcatg acaccggccc gggcagcctt ggatcgggtc
     5161 atatcccaac tggacggata tctcgactcc ggcctggagg acgatccgtt cctggtcctc
     5221 agtgtcccgg gcgatggcga gtcgtggcga gagagggccg agaaggtggt caccgagacg
     5281 attcggcccg ctgtccagga gtatcgagac tccttgatcc actatctcgc gcctaccgcc
     5341 cgccccgacg accgagtcgg cctgagctgg atcccgtatg gcgatgagat ctacgcccgg
     5401 ctcatcggga agtacacaca gctcgagaca tcggcttcgg cgatccacga catcggccag
     5461 gagtgggcta ccgggatcaa cgccggggag tgggtggaga tcgggagcaa ggcgttcggc
     5521 ctcgacagcc tcgaggccgt ctttgagcgg ctccactccg accccgacct ccgcttcagc
     5581 tccgaagagc acatgctcga acatgcccgc gttgcgctcg atcgagcatg gaaggaggtc
     5641 gacgcctggt tcgcccaccg cccggccacc ccgtgcaccg tggtgccagt tccggccgcg
     5701 gtagctcccg ccatgccacc ggcctactac agccagcctc cggtcgacgg atcacggccc
     5761 ggcacctact ttctcaacac ctacaagccc gaggagaggg atctgttcga gtacgaatcg
     5821 atccactttc acgagggtat tcccggccac cacttcgatc gctcactggc ggccgagctg
     5881 gaggggattc cgacctttcg ccggtacgcc atggtctatg cccacaccga aggatggggt
     5941 ctctactcgg agcgactcgc cgacgagatg ggtctgtact cgaacgagac cgaccggctg
     6001 gggatggtgt cagccgatgc ctggcgagcg ggccggctcg tcaccgacac cggtatgcat
     6061 gcgctgggat ggagccgtca acaggcgatc gactatctca tgcgttggac tccgataagc
     6121 cgcctgaccg tcgaacagga ggtcgatcgc tacatcggga tgcccggcca ggctctcgcc
     6181 tacaagatgg gccagctgga gatactccgt cttcgcgatg aagcgaggga ccgtctgggc
     6241 tcttccttcg acatcaaggg ctttcatgac acgatgctgg tcaatggggc catgaccctc
     6301 ccgatgctga gcaccgctgt agacgaatgg atgggtcgat cccctgacgt ttcaccaggt
     6361 gggccgtccg ggtactcact cgatgccacg accgaaggag aagacatggc caaggatcac
     6421 ggcccatcga tcaaggacga cgagcgatac gaagagctcc gcgaagaggg catgagcaaa
     6481 gagaaggccg cccgcatcgc caacacccct caggaagagg catccaagaa gggtggacaa
     6541 tcacctccct acgaggattg gacggtcgac gatctccgcg atcgtgccgc cgagataggg
     6601 atcgaagggc gctctgacat ggacaaggac gaactgatcg acgccttgcg aaaccactga
     6661 ccttctattc gtcgctggag tcgaacttga ccagcgggac gtagaaccag gcccatccgg
     6721 caatcgttgc cagcacgacg gtggccacga ccgccaccgg atcgctgaat acgagtgatg
     6781 acaccaggta gacgactgct gagatggcaa cgaggaaggc cactgtgccg acaatggcca
     6841 tcttcgtcgc atacatgatg tgactccacg tacgtcgttt gatccccgag atgggggcgc
     6901 ggactctctg atggacactt ggcgagatga ggagcaccga agccagggcg gcgctgccga
     6961 atgcgacata gaacacggcc ttgttgatcc caccgaggtc ggcgaacgac gcctgcagcg
     7021 gaaggatcaa gaggaacgag aacaagacca tgacgccggg gatggtggtg cgcagaccct
     7081 ccatcagcgc ccgaaactgg tcgtctatct cctcttggtc tttcggctcc ggctccccat
     7141 tcttcaatcg atgtgtcatc cggtacctct accccaacct tggaaaaggc aaccgtactg
     7201 gtttgagagg ggggtcatcc tgggtatcga tcacgtcggg cttcggacaa cggtgcgaga
     7261 aggtccgcag agccgccacg gcgatctcgg agagatcaac cgaaagagga agcaccggcc
     7321 aggtcgagga acgacgccgt cggctccctg aggacgcatt cgacgaaagg gctacgttga
     7381 gggacgaaga acatcgcttg ggggcacgac tcggcgagtt gcgagagttg gccagcggag
     7441 agggagcgct cccacaacgg caggccgcaa cgcagaccag tctgactcga acgccatcgc
     7501 tgcctcgatg atgagggcgg cagtatccca g
//
LOCUS       DASUTA010000186         2169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_185845, whole genome shotgun sequence.
ACCESSION   DASUTA010000186 DASUTA010000000
VERSION     DASUTA010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185845"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /gene="deoD"
                     /locus_tag="VFZ80_03735"
     CDS             complement(<1..431)
                     /gene="deoD"
                     /locus_tag="VFZ80_03735"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008957683.1"
                     /note="catalyzes the reversible phosphorolysis of
                     ribonucleosides and 2'- deoxyribonucleosides to the free
                     base and (2'-deoxy)ribose-1- phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX6286573.1"
                     /translation="MPTPTPHISASPGDFAPSVLLPGDPLRAKHIADNFLDDVKQVNQ
                     VRNMLGYTGSFDGLAVSVMGTGMGIPSASIYATELVNEYGVERLIRVGSCGAVTEKVD
                     VRDVVLAIGACTDSLVNRTRYGGLDFAATADFWLLEAAARAA"
     gene            504..938
                     /locus_tag="VFZ80_03740"
     CDS             504..938
                     /locus_tag="VFZ80_03740"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX6286574.1"
                     /translation="MPTRPDLDSIAESLRQATVHTFPGHVGLELVELGTGTAVMRCEI
                     QPFHMAPNGFLHAGVVITLADTAAGYGCVASWPEGATGFTTIELKTNFTGTLLQGTMR
                     AEASMVHGGRTTQVWDVDVTAEDDGSRLALFRATQMLLYPKT"
     gene            974..1468
                     /locus_tag="VFZ80_03745"
     CDS             974..1468
                     /locus_tag="VFZ80_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX6286575.1"
                     /translation="MSDTYDFIIGLRAIRDYRSEPLSSEDLGAILEAGRWTGSSKNRQ
                     DWSFIAVTDPDQIERLAEHGDYTQPVRDSAATIVLVQETGGNMFDIGRAAQNMMLAAW
                     AVGVASCPVTFHRHRDAAAQLGVPDGAIARYGIALGYPTDDAKPRNFGGRKKAAKVVK
                     LNSY"
     gene            1611..>2169
                     /locus_tag="VFZ80_03750"
     CDS             1611..>2169
                     /locus_tag="VFZ80_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286576.1"
                     /translation="MTQPLVQARGLTKRFGDFTAVDAVDFDIEKGEAFGFLGPNGAGK
                     TSTMKMITTVSPITEGKMTVFGIDPATDGRSIRQRIGVVPQEDNLDLELTVAENLYIY
                     GRYHDMKKSEINPRIEELLEFAQLTERRDSQVEPLSGGMKRRLTIARALINDPDLVIL
                     DEPTTGLDPQARQLLWDRLYGLKRRG"
BASE COUNT          438 a          703 c          660 g          368 t
ORIGIN      
        1 gccgctctgg ctgctgcttc cagcaaccag aagtctgccg tcgccgcgaa gtcgagaccc
       61 ccatacctcg tcctgttgac cagtgagtcg gtgcacgcgc cgatggcgag gaccacatcg
      121 cggacatcca ccttctcggt cacggcgcca cacgagccca ctcggatgag acgctccacc
      181 ccgtattcgt tcaccagctc ggtggcgtag atcgacgccg agggaatccc catgccggtc
      241 cccatgaccg agaccgccaa cccgtcgaac gaccccgtgt aaccgagcat gttgcgcacc
      301 tgattgactt gtttcacgtc gtcgaggaag ttgtcggcga tgtgcttggc ccgcagcggg
      361 tcgccgggca gcaacacgga tggtgcgaaa tcgcccgggg atgccgagat gtgcggcgtt
      421 ggcgtgggca tgtcagaaag gctcctggga taggggaacg tcgagagtca acctatctga
      481 tcacagaggt atcgtcgccg accatgccga ccagacctga tctcgactca atcgcagaga
      541 gtctccgtca agcgacggtg cacaccttcc ccgggcacgt cgggctggag ctggtcgaac
      601 tgggcacggg aaccgccgtg atgcgatgtg agatccagcc tttccacatg gcacccaacg
      661 ggtttctcca cgccggtgtc gtgatcaccc tggccgacac tgcagctggc tatggctgtg
      721 tcgccagctg gcccgaagga gcgaccggct tcacgacgat cgagctcaag accaacttca
      781 cgggcaccct tctgcaggga acgatgcgcg ccgaggccag catggttcac ggggggagga
      841 cgacccaggt atgggatgtc gatgtcaccg cggaggacga cggcagccga ctggcgctct
      901 ttcgcgcaac ccagatgctc ctctacccaa agacctgagc agccggtgcc gccacctggc
      961 ataggatcga cacatgtctg acacctacga cttcatcatc ggtctgagag cgattcggga
     1021 ctatcgctcc gagccgttga gcagcgaaga cctcggcgcc atcctcgagg ccggccggtg
     1081 gaccgggagc tcgaagaacc gacaggactg gtcattcatc gccgtgaccg acccggacca
     1141 gatcgaacgg ctggccgagc atggcgacta cacccaaccg gtccgcgact cggcggccac
     1201 catcgtcctg gttcaggaga cgggtggaaa catgttcgac atcggtcgag ctgcccagaa
     1261 catgatgctc gccgcctggg ccgttggcgt cgcctcttgt cccgtcacct tccaccgaca
     1321 ccgcgacgcg gcagctcagc tcggcgttcc cgacggggcc atcgcccgct acggcatcgc
     1381 cctcggctat ccgacggatg acgcaaagcc acgcaacttc ggtggccgaa agaaggcggc
     1441 gaaggtggtc aaactcaact cgtactgatg gactctgcga cctccggtag ttggagccat
     1501 atacggctcc aactacctcg agagtgcgct caccgggttc tgttcaccga ggttcagacg
     1561 tctttttcat gtgtgaactg tcacagcaag cggttaccgt cctgagactg atgactcaac
     1621 ctctcgtcca ggcacgaggg ctcaccaaga gattcggcga cttcacggcg gtagatgccg
     1681 tcgacttcga tatcgagaag ggtgaggcct tcggcttcct cggtccgaac ggagcgggga
     1741 agacatcgac gatgaagatg atcacgacgg tcagcccgat aaccgagggg aagatgaccg
     1801 tcttcgggat cgacccggcg acggacgggc gctccatccg tcaacgaatc ggcgtcgtcc
     1861 cccaagagga caacctcgat ctcgagctga cggtcgccga gaacctctac atctacgggc
     1921 gctatcacga catgaagaag tcggagatca acccgagaat cgaggagctg ttggagttcg
     1981 ctcagctcac tgagcgacgc gactcccagg tggaaccgct gtcgggtgga atgaagcgca
     2041 ggctcaccat cgcccgcgcc ctcatcaacg accccgatct ggtgatcctg gacgaaccca
     2101 ccaccgggct cgacccgcag gcccgccagc tgctctggga ccggctctac gggctgaagc
     2161 gcaggggcg
//
LOCUS       DASUTA010000187         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1739972, whole genome shotgun sequence.
ACCESSION   DASUTA010000187 DASUTA010000000
VERSION     DASUTA010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1739972"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFZ80_03755"
     CDS             <1..257
                     /locus_tag="VFZ80_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6286577.1"
                     /translation="SLAEEIERLRAEPGEGDIAIGGAALAAEAASLGLIDEYRAVVYP
                     VLVGGGIPFFPQHEHRVDLELVETRTFSSRFVYLRYRVAR"
     gene            836..1690
                     /locus_tag="VFZ80_03760"
     CDS             836..1690
                     /locus_tag="VFZ80_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286578.1"
                     /translation="MAEESDSVSVLETEVIGTAVSVYVVALSLWAARYLTEKSRQMES
                     VVRGLSEPGKDIRWLAGVDSTVGPVVLAVLLTVLEDIGLVESLDAGDLLVLALLQFVV
                     HLPLAAGVWVVWTILVSMYRMGRLDLRLDPHAGDPTLGLKPIGRLAVLTFGIFSAGAA
                     PILLANTDSGVALVINGSTFLIVVAVFVVALWGVHRQMMRVRDRAVGEARQRLQVIST
                     RIADAPESRLNEGDATLLMASDTLERRAREIAVWPLTGGLYVRTGAILLGVLIGLITR
                     AIGTALGV"
     gene            1747..2112
                     /locus_tag="VFZ80_03765"
     CDS             1747..2112
                     /locus_tag="VFZ80_03765"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX6286579.1"
                     /translation="MSRGSGTPFARDVSGLPAASRSRPLLVDDGATIDLIARPVSKQI
                     GDATVKMLGYDGSIPGPTLYVKQGSEATVRFGNETDQPTTVHWHGLRHDNLFACDSED
                     EPSITTAPGVSETTEPVGS"
BASE COUNT          409 a          707 c          759 g          483 t
ORIGIN      
        1 acagtctggc ggaggagatc gagcggttgc gagccgagcc gggggagggc gatatcgcga
       61 tcggcggcgc ggcgctcgcc gccgaggcgg catcgttggg tctgatcgat gaatatcggg
      121 ccgtggtata tccggtgctg gtcgggggcg gcatcccctt ctttccccag catgagcatc
      181 gggtggatct cgagctcgtc gagacccgca ccttcagttc gagattcgtc tacctccgct
      241 accgggtggc gcgctagcgc tcagacgatg aggcaaacga agaagacagc agctgattgg
      301 atgaccacag aaatcggatg tggctttggc gggtgtcgat tctgatcggg tttccgatcg
      361 gcgggtatgt cgcagacccg gtctggatgg agtcgactcg gtggaagcag cgattgccgc
      421 cggtcttatc acgggcgcaa tcattggagc ggcagaatgg cttgctttgc gtcggcgtgt
      481 ctcgtggctt tggattccgg cgacaacggc tgggatggcc gccggcctca ccgccggagc
      541 ggcattgcga gctcgtccgg ccgagcttct gctgtcccgg agttgcttcg gaaatgaagg
      601 ggctgaccaa tggattgagg aatagccttc cgacgatgag tcgacccacc cggcgcgcca
      661 gtaccaattc atcagactgg cgtcaacatg ccgagccatc agcaagccac gcccctcttg
      721 tgaaccagga ccacgtccta accgagcgcg tgatggttgg ctaccggaac cgagaatgct
      781 gtggctggtg gtttgggccc tcacaccggt tggtttgaga acagtgctcg gcgctatggc
      841 agaagagtcc gactctgtct cggtgctcga gaccgaggtg atcggcaccg ctgtcagcgt
      901 ctacgtcgta gcgttgtctc tgtgggcggc tcgctacttg acagagaagt cacgccagat
      961 ggagagcgtc gtgagaggac tctccgaacc gggcaaagac atacgctggc tcgccggggt
     1021 cgacagcacg gtcggacctg ttgttctcgc cgtgttgttg accgtcctcg aggatatcgg
     1081 gctggtggaa tcgctcgacg ccggagacct tctcgtgctc gcgctactcc agttcgtcgt
     1141 ccatctcccc ttggcagccg gggtctgggt ggtgtggacc atcctcgtct cgatgtatcg
     1201 aatgggtcgt cttgatctgc ggttggatcc gcacgccggg gaccccacgt tgggactcaa
     1261 gccgatagga cggttggccg tgctcacttt tggcatcttc tcggcaggtg ctgccccgat
     1321 tctcctggcg aacacggata gtggtgttgc cctggtcatc aatggctcca ccttcctcat
     1381 cgtcgttgca gtattcgttg ttgccctctg gggagtccat cgccagatga tgcgggtgcg
     1441 ggaccgagct gtcggcgagg cgcgtcagag gctccaggtg atctcgaccc gcattgcaga
     1501 tgcccctgag tcgagattga atgaaggaga cgcgactctc ttgatggcca gcgacaccct
     1561 cgagcgtcgc gcccgcgaga tcgcggtgtg gccattgact ggaggtctct acgttcgaac
     1621 cggcgccatt ctcctcggtg tcctcatcgg tctcatcacc agagcgatcg gcaccgccct
     1681 gggggtgtga cgagggagtg gtctaggaat gcccgaagta gccaagtgag taccgagcac
     1741 cacggaatga gccgcgggtc gggaacgccc tttgcgcgtg acgtctccgg tctgcccgcc
     1801 gcctcccgtt cccgtcctct cctggtggac gacggcgcaa cgatcgatct catcgctcgt
     1861 ccggtcagca agcagatcgg tgacgccacc gtcaagatgc tcggatacga cgggtcgatc
     1921 ccggggccca ccttgtacgt gaagcaaggc tcggaggcga cagtccgttt cggaaacgag
     1981 acggaccaac cgaccaccgt gcactggcac ggtcttcgtc acgacaatct gttcgcatgt
     2041 gactccgaag acgagccctc gatcacgacc gcgccgggcg tctccgaaac caccgagccc
     2101 gtcggttcct aaacctcctc ggggatagtc gagctctgcg tcctgtggtc aatcggccca
     2161 gctagtcgcg tgattccggg tgtgctcccc tggcagcttc ctttttcgcg ccccaaacca
     2221 cctctcgccg aaagggccag tttggcactt gacacggtgc caaaatggtg ccaatatggt
     2281 gtcatgaacc ttgccacaca tgtggagaca ctgaggcagc atctggcggc cgctgccgag
     2341 acaggcggct ccgaggcc
//
LOCUS       DASUTA010000188         2160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_316501, whole genome shotgun sequence.
ACCESSION   DASUTA010000188 DASUTA010000000
VERSION     DASUTA010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316501"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="VFZ80_03770"
     CDS             <1..1336
                     /locus_tag="VFZ80_03770"
                     /inference="COORDINATES: protein motif:HMM:NF040246.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEX6286580.1"
                     /translation="TENTRDCYAALGLVHSHWVPVTGRFKDYVAMPKINLYQSLHTTI
                     IGPDGKPLEVQIRTVEMHRRAESGIAAHWRYKEGDIDARSLLDPESFDEDPEEFLDNL
                     KMDLYLDEVFVLTPDGDVKELPKSATPVDFAYAVHTEVGHRCVGAKVNGRLVPLSTKL
                     DSGDIVEIITSNSPDAAPSRDWLKFVRTGKARNKIRQWFQKERRDQAITDGREQIQSL
                     LKKEVHELTPAEQARHLAEIAEELGQRDLEALQVAVGEGNVSLDSVAVRLHRRLEPDI
                     GDDLFRPTRRRRPSESAHVVVEGQDDMLVSLAQCCAPVPGDPIVGYVTVGRGVSVHRS
                     DCTNVSSLAQRRERSVEVSWPSDASGAFVVWIQVEALDRARLLRDVTSVVSDTGGNIT
                     ASSTNTGEDRVAILKYEVELSDPSQLPRLLSDIRGVDGVYTAFRLANDPAAS"
     gene            1336..1980
                     /locus_tag="VFZ80_03775"
     CDS             1336..1980
                     /locus_tag="VFZ80_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6286581.1"
                     /translation="MDIQRIPAWVTGTNCYVATSDQSHAVLIDAPPEPDLIGQRLARM
                     GVAVVGILLTHGHVDHTGGAGKLAAQSGAITYVHPADDFLTLHPTEQLERIFGVVPPG
                     SYEVPDSFQSLAHGQRLELSGMSFEVRHTPGHTPGHCCFFVEEEGVLFSGDQLFKGSI
                     GRFDLPGGSFEDLMESMRAQVMTLDDDVEVLPGHGDETTIGRERATNPFRAEWG"
BASE COUNT          407 a          702 c          680 g          371 t
ORIGIN      
        1 caccgagaac acacgcgact gctatgccgc cctcggcctg gtccacagtc attgggtccc
       61 ggtcaccggg cgcttcaagg actacgtggc aatgcccaag atcaatctct accagtcgct
      121 gcacaccacg atcatcggcc ccgacggcaa accgctcgaa gtccagattc gaacggtgga
      181 gatgcaccgc cgcgccgaat ccgggatcgc cgcccattgg cgatacaagg aaggcgacat
      241 cgacgccagg tccctgctcg atccggagtc gttcgacgag gaccccgaag agttcctcga
      301 caacctgaag atggacctct acctggacga ggtattcgtc ctgacaccgg acggcgacgt
      361 caaagagctg cccaagagcg ccacacccgt cgacttcgcc tatgcggtgc acaccgaggt
      421 tggccatcgc tgtgtcggag ccaaggtgaa cggtcgactc gtcccgttgt cgacgaaact
      481 cgactcaggg gacatcgtcg agatcatcac ctccaactct ccggacgcgg cgccctcacg
      541 tgactggctc aagttcgtcc gtaccggcaa ggctcgaaac aagatcaggc agtggttcca
      601 gaaagagcga cgcgatcagg cgataaccga cggccgcgag cagatccagt cgctgttgaa
      661 gaaggaagtg cacgaactga cccctgccga gcaggcccgg cacctggcgg agatcgccga
      721 ggagctcggc cagagagatc tggaggcgtt gcaggtggcg gtgggcgagg gcaacgtctc
      781 gctggactcc gtcgcggtca ggctccatcg ccggctcgag ccggacatcg gcgacgacct
      841 cttccggccg acacgacggc gacgcccatc cgagtcggca cacgtggttg tcgaggggca
      901 ggacgacatg ttggtgtccc ttgcccagtg ctgtgcgccg gtgccgggcg atccgatcgt
      961 cgggtatgtc acggtcgggc gcggtgtctc ggttcatcga tccgactgca ccaatgtctc
     1021 ctcgctcgcc cagcgccgcg agcgatctgt cgaagtgagc tggcccagcg atgccagtgg
     1081 ggcgttcgtg gtctggatcc aggtcgaggc tctcgaccgg gcccgcctcc tccgtgacgt
     1141 gacttccgtg gtgtccgaca ccggagggaa catcactgcc tcttccacca acaccggcga
     1201 ggatcgggtg gccatcctca aatacgaagt cgagctctcg gacccgagtc agctcccgcg
     1261 tctgctcagt gacattcgcg gagtggacgg cgtgtacacc gcgttcaggc tggccaacga
     1321 tccggccgcg tcctgatgga tatccagcgc attccggcat gggtgaccgg gaccaattgc
     1381 tacgtcgcca cctcggacca gagtcacgcg gtcttgatcg atgcgcctcc ggagcccgac
     1441 ctcatcgggc aacggttggc gcggatgggt gtcgctgtcg tcggcatcct tctcacccat
     1501 ggccatgtcg atcacaccgg aggcgcaggc aaactcgccg cgcagagtgg agcgatcacg
     1561 tatgtccacc ccgccgacga cttcctgact ttgcacccga cggagcagct ggagaggatc
     1621 ttcggagtgg ttccaccggg atcctatgaa gtgcccgact cgttccagtc tctggcacat
     1681 ggccagcggc tcgaattgag cgggatgagt ttcgaagtcc gtcacacccc gggtcacacc
     1741 ccggggcact gctgcttctt cgtcgaggag gaaggagtct tgttcagcgg cgaccagctc
     1801 ttcaaaggaa gcatcgggcg cttcgatctg cccggaggct cattcgagga cctgatggag
     1861 tcgatgcggg cccaggtcat gaccctcgat gacgacgtcg aggtgctccc gggtcacggt
     1921 gacgagacga cgatcggccg cgagcgggcc accaacccgt tccgggcaga gtggggatga
     1981 cggccaatcg acatgtgaac cgccgggtcc gtcggtaacc tgcgccgcaa tccatgagct
     2041 tccagccccc caaaggcacc gatgacatca tcgcgcccgc gtcgcaggcg tggcgtgatg
     2101 cgttgcgctc ctgggagaca tgggcagagc gctatggcta ccccctgatc agcttgccga
//
LOCUS       DASUTA010000189         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_444267, whole genome shotgun sequence.
ACCESSION   DASUTA010000189 DASUTA010000000
VERSION     DASUTA010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444267"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VFZ80_03780"
     CDS             complement(<1..268)
                     /locus_tag="VFZ80_03780"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of L-histidinol phosphate
                     from imidazole-acetol phosphate and glutamate in histidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase"
                     /protein_id="HEX6286582.1"
                     /translation="MPRVRADIAAIRPYQVGRQLADVARAHGLDPSDIVKLTANEGPE
                     GPFPGVVEAATAVIAGSNRYPDNDCWDLGHELADELGVDFSNLMF"
     gene            326..>2376
                     /gene="pdhA"
                     /locus_tag="VFZ80_03785"
     CDS             326..>2376
                     /gene="pdhA"
                     /locus_tag="VFZ80_03785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX6286583.1"
                     /translation="MGHIETLRIIDAQGELVGDDPGLDPELYQTMYRNMVLSRTLDRR
                     MLALQRQGRLGTYAMLEGQEAVQIGSALALDPSDFVFPSYREHGVQVTRGLPMEVLLA
                     YWKGLPNAAWDIEKYRTGIVTVPIASQLPHAVGYSYMTKLRGEDTVTVTYFGDGATSE
                     ADFHSGMNFAGVWKTPTVFICANNLYAISVPYEKQTASETIAQKAGAYGFEGIRVDGM
                     DPIAMYLATRAAVQRARNGDGPTLIEALTYRYGPHATADDPTLYRSEEEVEEWKEKDA
                     IERLRRFLENRGEWDERVGEKVAMEITDSVDAAVTSLEAEPMPGRDDAVRHGFARIPP
                     HVIEQLHAMQRSHGEPETEFSESEVWHVGTDITPEGPTKSMTMAEAINAALDEAMAAN
                     EEVIVLGEDVGKAGGVFRVTEHLQKRYGEDRVIDTPLNESGIVGTAIGMALAGARPVA
                     EIQFDGFVYPAFDQIVSHLGRFRFRTRGLSAVPVIVRFPNGAGIGAHEHHCDSPEAYF
                     VHAPGLVVVCPSTAFDAKGLMAAAFAGEDPVIFLEPKVLYRAGRNDVPTGEYTLPIGR
                     ARVRLEGNDLTLVTYGGMVPVSIKAAEQSDHSIEVIDLRTLFPWDRETVLSSVAKTGR
                     LLLIQEPQGSAGVAAEIAAVVSEQAMYDLAAPIVRVTGFDVPWPQFAIEKHALVDVAR
                     V"
BASE COUNT          460 a          755 c          761 g          400 t
ORIGIN      
        1 caaacatgag gttggagaag tcgacaccga gctcatcggc tagctcatga ccgagatccc
       61 aacaatcgtt gtccggatac ctgttggagc cggcgatgac cgccgtggcg gcctcgacga
      121 cacctggaaa aggcccttcc gggccctcat ttgccgtgag cttgacgatg tccgacggat
      181 cgagcccgtg ggcgcgggcc acatcggcta gctgacgccc gacctggtag gggcggatcg
      241 ccgcgatgtc ggctcggacc ctgggcatat cgctcgaaga tagcctcacg ctccggatga
      301 cgtgctcatg tggcagactc acctcatggg ccacatcgag acgttgcgga tcatcgacgc
      361 ccaaggagag ctggttggag acgacccggg gctggacccc gagctctatc agaccatgta
      421 ccgcaacatg gtcttgtccc ggacactgga ccgacggatg ctggcgttgc agagacaggg
      481 gaggctgggg acctacgcca tgctcgaagg ccaggaggcc gtccagatcg gctcggctct
      541 cgccctcgac cccagcgact tcgtgttccc aagctaccgc gagcatggag tccaggtgac
      601 caggggactt ccgatggaag tgctcctcgc ctattggaaa ggccttccca acgccgcctg
      661 ggacatcgag aagtaccgca ccgggatcgt cacggtgccg atcgccagcc agctccccca
      721 tgccgttggc tactcctaca tgaccaagtt gcggggcgag gacacggtca ctgtcaccta
      781 tttcggagat ggcgcgacat cggaggccga tttccactcc ggaatgaact tcgcaggggt
      841 ctggaagaca ccaaccgtct tcatctgtgc caacaacctc tatgccatct ctgtgcccta
      901 tgagaaacag actgcgtcgg agaccatcgc ccagaaggcg ggggcctacg ggttcgaagg
      961 tattcgggtc gacggtatgg atcccattgc catgtatctg gccacccgcg cagcggtcca
     1021 gcgggcgaga aacggagatg ggccgacact gatcgaggcg cttacctatc gctacgggcc
     1081 gcacgccaca gcagacgacc ccaccctcta tcgatccgaa gaagaggtcg aggagtggaa
     1141 ggagaaggac gccatcgagc gtctgcgccg attcctcgag aatcgcgggg agtgggacga
     1201 gcgagtgggc gagaaggtgg cgatggagat caccgactcg gtcgatgccg ccgtcacctc
     1261 gctcgaagcc gagcccatgc cagggcggga cgatgcggta cgtcatggct tcgcccggat
     1321 cccgccgcac gtcatcgagc aactgcatgc catgcagcgc tcccacggag agcccgagac
     1381 ggagttctcg gagagcgagg tctggcacgt gggcacggac atcaccccgg aagggcccac
     1441 caagtcgatg acgatggccg aggccatcaa cgccgcgctc gacgaggcga tggccgccaa
     1501 cgaggaagtg atcgtccttg gggaggatgt cgggaaagcg ggtggcgtat tcagggtgac
     1561 cgagcatctc cagaagaggt acggcgagga ccgtgtcatc gacaccccgc tcaacgagtc
     1621 cggcatcgtc ggtaccgcga tcggcatggc actggccggg gcaagaccgg tggccgagat
     1681 ccagttcgac ggattcgtct atccggcgtt cgaccagatc gtgagtcacc tcgggcgatt
     1741 ccggttccgc acccgagggc tgtcggcggt cccagtcatc gttcgcttcc cgaacggcgc
     1801 cggtatcggg gcacacgagc accattgcga ctcgcccgag gcgtatttcg tacacgctcc
     1861 cgggctcgtt gtcgtgtgtc cgtcgacggc cttcgacgcc aaaggcctca tggcggcggc
     1921 gttcgccggc gaggaccctg tcatcttcct cgagcccaag gtgctctacc gggcgggccg
     1981 gaatgacgtc cccaccggtg agtacacgct acccatcggt cgagcccggg tccgactgga
     2041 aggaaacgac ctgaccctcg tcacctatgg gggaatggtg cccgtctcca tcaaggccgc
     2101 ggagcaatca gaccactcga tcgaagtcat cgatttgcgg acactcttcc cctgggaccg
     2161 ggagaccgtc ctcagctccg ttgccaagac cggccgtctg ctgttgatcc aggagccgca
     2221 gggcagtgcc ggcgtcgcag ccgagatcgc agccgtggtc tccgagcagg ccatgtacga
     2281 cctggctgcc ccgatcgtga gagtcaccgg attcgatgtt ccctggccgc agttcgccat
     2341 cgagaagcat gccctcgtcg atgtcgccag ggtcaa
//
LOCUS       DASUTA010000190         3145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1740250, whole genome shotgun sequence.
ACCESSION   DASUTA010000190 DASUTA010000000
VERSION     DASUTA010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740250"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="VFZ80_03790"
     CDS             complement(<1..1168)
                     /locus_tag="VFZ80_03790"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX6286584.1"
                     /translation="MTASAVAIIAFLILGWAVVSAALARRDITGPLIFSVFGYVLANP
                     DWGPIPIDVEAASIHLIAEATLALVLFSDASRVNLQELRRDPSLPLRLLGMGLPLTLL
                     LGGLLAAVLFGEFTWAIAGFVAAALAPTDAALSVQVINDERVPLRLRSGLNVESGLND
                     GIVTPIVTFTLAIAASQLGVAAESESHATGTALRELGLGIVAGVVVGLVGAVLINATS
                     RRRWIAPGGRRLATLAMAVGAFAVALAFDANGFIAAFVAGMAFGATLDEDVVDVEAAI
                     QLPELGGELLALVVWFFFGATLVPIAFNYLDAPVIGYALLSLTLIRMLPVILAMLRTG
                     LGRTEVVFLAWFGPRGLASVVFALLALEELGTSSDIVRQAVAVVALTVLFSVVLH"
     gene            complement(1292..2983)
                     /locus_tag="VFZ80_03795"
     CDS             complement(1292..2983)
                     /locus_tag="VFZ80_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX6286585.1"
                     /translation="MMTSPGKRHWLERKSVRRDATAGLVVGIESVPDGLAGGLLAGVN
                     PVYGLYAYLVGTVTGAIFTSSAFMAVQATGAMAIIVADVDVVHDAEDPGRALFTLSIV
                     TGVIMLAAGLLKLGSTLRFVSNAVMVGFISAVGVNIILGQLADFTGYEGEGGNRVVRA
                     LDTLLNLRQADGATMLIGVATIALIVMLERSRLGPLGMVVAIVATSAAVGVFGLDTVA
                     QLGDIADIPSSLPRPTWPLLGLVPALLIPAFSLAFVGLVQGASITANFPNPDGTYPDA
                     SRDFVGQGAANVAAGVFQAMPVGGSMSGTSLAVSAGARSRLALILATVVMAVVILVFG
                     DAVSLIAMPALAGLLMLVGYRTIKPGNMVSVWKTGRVQAVVLAVTFGLTMLIPLQYAV
                     LVGVGLSVVLHVIRQSNQVTIKQRVFEPSGEVTETAPPGLLPHDEVIVLQPYGSLFFA
                     SAPIFEKQMPEVTATSRNTVVILRLRGRSDLGTTFMDVLRRYALSLQDVDSKLMLVST
                     DRILEEQFAVTGVTEVVGADNIYSGTERIGTTLKRAHSDAVAWVAANKALGSEPA"
BASE COUNT          624 a         1004 c          989 g          528 t
ORIGIN      
        1 cgtgaaggac cacactgaac agcacggtga gtgctactac cgccaccgcc tggcgcacga
       61 tgtcactgct cgtccccagt tcctcaagtg cgagtagggc gaacacgact gatgcgaggc
      121 ctcgtggccc gaaccaggcg aggaagacga cttcggttcg gcccaaaccc gtccgcagca
      181 ttgccagaat gaccggcagc atgcggatga gggtcaggct gagtagcgca tatccgatca
      241 ccggggcgtc gaggtagttg aacgcgatcg ggaccagggt cgccccgaag aagaaccaca
      301 ccaccagggc gagaagctca ccgccaagct cgggaagctg gatcgctgct tcaacgtcga
      361 cgacgtcttc gtccagagtt gccccgaagg ccatgcctgc cacgaaggct gcgatgaatc
      421 cgttggcgtc gaaagcgaga gcaacggcga atgcgccgac tgccatcgcg agagtcgcca
      481 gtcgccggcc accgggcgcg atccaccttc ggcgagaggt ggcgttgatc agaactgcgc
      541 cgaccaagcc gacaaccacg ccagcaacga tccccaaacc gagttcgcgt aatgcggtac
      601 cagtcgcgtg tgactcgctc tctgcggcga ctccgagttg gctggcggcg atcgcgagcg
      661 tgaaggtgac gatgggcgtg acgatcccgt cgttgagccc actctcgacg ttgagcccgc
      721 tccgaagtcg taatggcacg cgctcgtcat tgatcacctg cacgctgaga gcggcgtcgg
      781 tcggggcgag ggcggcagcc acgaagccgg cgatggccca ggtgaactcc ccgaatagca
      841 cggctgccaa cagacctccg agcaacagag tgagcggaag gcccatgccg aggagacgga
      901 gcggcagcga cgggtctctt cgcagctctt ggagattgac cctggaagcg tccgagaaca
      961 gcacgagggc cagcgtggct tcggcgatga gatggatcga cgcagcctcc acatcgatcg
     1021 ggatcggtcc ccaatccggg ttggccagga cgtagccgaa aacagagaag atgagcgggc
     1081 cggtgatgtc gcggcgggcc agtgccgccg acacgaccgc ccaccccagg attaggaaag
     1141 cgatgatggc cactgcggag gcggtcatgt ttcctcccca ctcattgtgt gaggaatcat
     1201 gcagcccgcc ttctcacgtt cgtcgacccc agcatggacg cattggtgag tcccgtcatc
     1261 gtcttgtcgg tgtgtttgcc gcgcttgtgt ctcacgctgg ctccgacccc agggccttgt
     1321 tcgccgccac ccaggcaacc gcgtcggaat gtgctcgttt gagggtggtg ccgatccgct
     1381 cggtcccgga gtagatgttg tccgccccga ctacctctgt gactcctgtc accgcgaact
     1441 gctcttcgag gatcctgtcg gtggagacca gcatcagctt gctgtcgacg tcctgcaggg
     1501 atagcgcata tcgccgcaag acgtccatga aggtagtgcc gaggtcgctt cggccccgca
     1561 agcggaggat cacgacagtg ttgcgggagg tggcggtcac ctccggcatc tgcttctcga
     1621 atattggcgc cgacgcgaag aacaggctcc catatggctg gaggacgatc acttcgtcgt
     1681 gtgggaggag gccgggtggt gctgtctccg tgacctcccc tgagggctcg aatacccgct
     1741 gcttgatcgt gacctgattg gactggcgaa tcacgtggag cacgactgac aggccgactc
     1801 cgaccaacac ggcgtattgg agggggatga gcatggtcag cccgaaggtc acggcgagca
     1861 ctactgcctg tacccttcct gtcttccata ccgacaccat gttcccgggt ttgatggtgc
     1921 gatacccgac gagcataagc agcccggcaa gggccggcat agcgatcagc gaaacggcat
     1981 cgccgaagac caagatcacc actgccatta cgacggtggc gaggatcaga gccagccgcg
     2041 atcttgcgcc tgcggacacg gccagtgacg tcccggacat cgagcctccg accggcatcg
     2101 cctgaaacac tccggctgcc acgttggcgg cgccctggcc gacgaagtct cgtgatgcgt
     2161 cgggatacgt cccgtcggga ttggggaagt tggccgtgat gctcgctccc tgcaccagac
     2221 cgacgaacgc caatgagaag gcaggtatca gtagcgccgg caccagcccg agcagcggcc
     2281 aggtcggtct cggcaacgaa ctgggtatgt ctgcgatgtc cccgagttga gcgacagtgt
     2341 ccagaccaaa caccccgacg gcggcagacg tggcaacgat ggcaaccacc attcccagcg
     2401 ggccgagccg gcttcgttcg agcatcacga tgagtgcgat ggtggccacc ccgatcagca
     2461 tcgtcgcacc atccgcttga cgaaggttga ggagcgtgtc cagagcgcga acgacacgat
     2521 ttcccccctc gccctcgtat ccggtgaagt cggcgagctg accgagaatg atgttcacgc
     2581 caaccgcgct gatgaacccg accatcaccg cgttggagac gaatctgaga gtcgagccca
     2641 gcttgaggag gccggcggcg agcatgatga ctcccgtgac gatcgacagg gtgaacaggg
     2701 cgcgacccgg gtcctcggca tcatgcacta cgtcgacatc ggcgacgatg atcgccatcg
     2761 ccccggtcgc ctgaacggcc atgaaggcgg agctggtgaa gatcgccccg gtcacggttc
     2821 ctaccaggta ggcatacagt ccgtagaccg ggttgacccc ggcgaggaga ccaccggcga
     2881 gcccatccgg gacactctcg atccccacaa cgagccccgc cgtggcgtca cgtcgcaccg
     2941 acttacgctc caaccagtgc cgcttgcccg gcgaggtcat catccacctt cctggctgcc
     3001 gagcgccgat tgtctaggac tcggctgcag atttatcact tggctcctgg ccttctacca
     3061 accatccacc ggctcgtcct tcatcgaccg ttggggatga gcttgggagt cccgatggaa
     3121 gcaggtccat cacctctggc gcacc
//
LOCUS       DASUTA010000191         4180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1352673, whole genome shotgun sequence.
ACCESSION   DASUTA010000191 DASUTA010000000
VERSION     DASUTA010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352673"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1460)
                     /locus_tag="VFZ80_03800"
     CDS             complement(<1..1460)
                     /locus_tag="VFZ80_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX6286586.1"
                     /translation="MGGAADTSAPSRLVARVPIAATLRDYRREWIRPDLLAGLVLATM
                     LVPQGMAYADLTGLPAVTGIYTTIAALIAYGIFGPNRRLVLGPDSSLAPVIAAVVVPL
                     AAGADDAVALASAMSLMAGLVCVLAGFLKLGVVTELLSKPIRIGYLNGIAVLIFLSQL
                     PAALGLEVDGTSPLEILTGTLDLIRDGQIVPQAVTLSVAGVVVIVVLNRLSRLRPGVI
                     VAAVGSIVAVSVFGLAVEVVGDIPSGLPPLTIPAIDGDVITALVGGALAVALVSFADT
                     GSLSTATALKAGEKVDPNSEIKALGASNLLSGLFQGFATSASSSRTAVALAVGSRTQV
                     AGLIAAAGVVLIVLVFPGLVSEMPQPTLAAIVITASFTLFDWRGWLWLSRVRRSEFFL
                     STAAALAVLFVGVLEGIGIAILLSLANFIRRSWRPHSAELGKVPGVPGYHDRERHPEA
                     ETVPGLLIIRYDAPLFFANAPDFGRTLQEMLRETDRP"
     gene            complement(1573..1899)
                     /locus_tag="VFZ80_03805"
     CDS             complement(1573..1899)
                     /locus_tag="VFZ80_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286587.1"
                     /translation="MTNERRTPGIRDVWRLILAVIGVIAVIQELRKPAEERTWHGKVA
                     DFVPYDFRMPTMTRVRDTYWNRGGPLLSSKVFGVGWAPNFGALLAILGKDDESSSEDS
                     GEDPAA"
     gene            complement(1974..2273)
                     /locus_tag="VFZ80_03810"
     CDS             complement(1974..2273)
                     /locus_tag="VFZ80_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015749151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286588.1"
                     /translation="MCCLFTTLVLVGPRAAIIVWGLAQPARWDLAFSTFVWPLLGFVF
                     LPWTTLMYVGVAPGGVSGFDWFWIVIAVVIDLASYSGSVYGNRDRIKGYGPMAPA"
     gene            2420..2818
                     /locus_tag="VFZ80_03815"
     CDS             2420..2818
                     /locus_tag="VFZ80_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEX6286589.1"
                     /translation="MTRHHYGFGVETSLTPAEAEMRIRDALADEGFGILTEIDVKTTL
                     KNKLDIDIAPYRILGACNPQLAHQALTAEQSIGLLLPCNVIVYETDDGSAIEVLEPNL
                     MAELTDNPDLRELAKDARARLERALAKVES"
     gene            2889..4172
                     /locus_tag="VFZ80_03820"
     CDS             2889..4172
                     /locus_tag="VFZ80_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6286590.1"
                     /translation="MNSASEVTSDGFMGRLERRYRPAPGAVFLEDPTMMVRLPLINGA
                     SVVANTVIIATLLFTNGESAVGWTVVALGVTYLLAVLAFLITGRAAVYIHGVLWPSLV
                     QNVAAHVMLGGFIWSGATLFWGIIVSVVAALFLGRRPGAILAGSYGVAAIVFAFLEPT
                     LQARRSQPALVVSVGMAVDVFVISLLILVPIVMSLMGQVIRERGRTESLLLNVLPETI
                     AGRLKLSPGVIADDYEGCTVMFADLVGFTDHTSRISPEQLITELNLIFSRFDALVHDL
                     GAEKIKTIGDGYLAVAGAPIRRPDHAELMCELALRMQETMPEINEELGSSLQLRIGLH
                     TGPLVAGVVGVSRFAYDLWGHTVNLASRMETVSPPGGIRATDAVARAAGDRYVFEQHG
                     FSDVKGVGPVATCLLVGRKQVQVEQPALAHPETLS"
BASE COUNT          813 a         1375 c         1300 g          692 t
ORIGIN      
        1 ggccggtccg tctcccgtag catctcctga agggttcgcc cgaagtcagg cgcattggcg
       61 aagaacaagg gtgcgtcgta gcggatgatg agcaacccgg gcaccgtctc ggcctcggga
      121 tgacgctctc ggtcgtggta accagggaca cctgggactt ttcccagctc ggcgctgtgg
      181 ggccgccacg accggcggat gaagttggcc agcgagagca ggatggcgat gcctatcccc
      241 tcgagcacgc ccacgaagag gactgccaga gccgctgcgg tcgagaggaa gaactccgag
      301 cgacgcaccc gcgacagcca cagccaacct ctccagtcga aaagtgtgaa cgacgccgtg
      361 atcacgatcg cagccagtgt gggctggggc atctcggaca ccagtcccgg aaacacgagg
      421 acgatgagga ctactccggc cgcggcgatg agaccggcca cctgggtgcg tgagccgacc
      481 gccagcgcca ccgctgttcg cgacgagctg gcactggtgg caaaaccctg gaacaggccg
      541 gataagaggt tggaggcgcc cagggccttg atctcgctgt tcgggtcgac cttctctccc
      601 gctttcagag ccgtcgccgt cgagagtgaa ccggtgtctg cgaacgacac caaagcgacc
      661 gccagtgctc caccgacgag ggcggtgatg acgtcaccgt cgatggcagg gatggtcaga
      721 ggaggcaacc ctgagggaat gtcgccgacg acctcgaccg cgaggccgaa cacactgacg
      781 gccactatcg agccgaccgc agccacgatc acaccaggcc gaagccggct gagacgattg
      841 agcacaacga tgaccaccac accggcgaca gacagtgtca ccgcctgcgg cacgatctgg
      901 ccgtcacgaa tcagatccaa cgtgccggtc agtatctcca aaggcgacgt gccgtcgacc
      961 tcgaggccta gagccgcggg gagctgggag aggaatatga gcacggcgat gccgttcagg
     1021 tagccgatcc gaatcggctt ggagaggagc tcagtgacca ctccgagttt gaggaacccg
     1081 gcgagcacgc acaccagccc ggccatgagg ctcatcgccg aggccagggc gacggcgtcg
     1141 tcggctcctg cggcgagagg gacgaccacg gccgcgatca ctggagccaa cgacgagtcg
     1201 ggcccgagca cgagccgacg gttgggtccg aagatgccat aggcgatcag cgcggcgatc
     1261 gtcgtgtaga tgccggtcac cgcaggcagg ccagtcagat cggcatatgc catcccctgg
     1321 ggcaccagca tcgtggccag gaccagaccg gcgagaaggt cgggacgaat ccactcacgt
     1381 cggtagtccc gaagcgttgc cgctatcgga acccgagcga cgagacggct tggcgcgctg
     1441 gtgtctgctg ctcctcccaa gaggctttca agttaccgcc ggaacggtcc ggcaacgcgg
     1501 gcatggcaaa tcgtgatgat gcgatcgccc gggtgccgga cccgggctcc tcaggtccta
     1561 gtcggggacg attcaagccg ccggatcttc gccagagtcc tcgctggagc tctcgtcatc
     1621 ctttcccaga attgcgagga gggctccgaa gttgggggcc cagccgacac cgaagacctt
     1681 cgacgacagc agagggccgc ctcggttcca gtaggtgtca cgaaccctgg tcatcgtggg
     1741 catgcggaaa tcgtagggca cgaagtcggc gaccttgcca tgccaggtcc gctcctccgc
     1801 cggcttccgc aactcctgga tgacggcgat gacaccgatg accgcgagga tcagtctcca
     1861 aacgtcccgg atgccgggtg ttctccgttc gttggtcatg ccggcagagt acctaacacc
     1921 acgacgacac agtcacgcct tggatcattc cggaaaattc gctgctcttc cgctcaggca
     1981 ggtgccatcg ggccgtaacc cttgatccgg tcgcggttcc cgtagacgct ccccgagtac
     2041 gaggcaaggt cgatcacgac ggcgatgacg atccagaacc agtcgaagcc gctgacccca
     2101 ccgggggcga ccccgacgta catcagagtg gtccaaggga gaaacacgaa cccgagcaac
     2161 ggccagacga aggtggaaaa ggccaggtcc catctcgccg gctgagccaa cccccacacg
     2221 attatcgccg cccgcggccc tacgagaacc aacgtggtga acaaacaaca catgtcatat
     2281 tcccttccgc ggccaccgcc gcagcactgc acacgggccc tcagcccgcc tggaagttac
     2341 acgatgccca cttggtcggg tcgagcactt tccccgctga cgtcgtagcc tccgatgacc
     2401 gacgcatcag gaggcaaccg tgaccagaca tcactacgga ttcggtgtgg agacatcgct
     2461 caccccagca gaggccgaaa tgagaattcg tgacgcgctg gcagacgagg ggtttggcat
     2521 tctcaccgag atcgacgtca agaccacgct caagaacaag ctcgacatcg acatcgcccc
     2581 ctacagaatt ctcggcgctt gcaaccccca actcgcccac caggccctga ccgccgagca
     2641 gagcatcggt ctgctgctgc cctgcaacgt gatcgtctac gagaccgatg acgggagcgc
     2701 catcgaggtc ctcgagccga acctgatggc cgagctcacc gacaatcccg acctccgaga
     2761 gctcgccaag gacgcccggg ccaggctcga acgggctctc gccaaggtgg agagctgacg
     2821 gcgccggacg gccttgatca tcgattcctg cgagaaatca atcatcggga agtccgtaga
     2881 attcgccggt gaattcggct tccgaagtga catccgacgg cttcatgggg cgactggagc
     2941 ggcgttaccg gccggcgccg ggagccgtct tcctcgagga ccccaccatg atggtgcggc
     3001 tccccctgat caacggcgcc tccgtggtgg ccaacactgt gatcatcgcc acactgctgt
     3061 tcacaaacgg ggaatcagca gtgggttgga ccgtggtcgc cctcggtgtc acctacctgc
     3121 tcgccgtgct cgccttcctc atcacggggc gggcggccgt gtacatccat ggggtcctct
     3181 ggcccagcct ggtgcagaac gtggctgcgc atgtgatgct cgggggcttc atctggtcag
     3241 gcgccacgct cttctgggga atcatcgtct ccgttgtcgc tgccctcttc ctgggccgtc
     3301 gccccggggc gattctcgcc ggttcctacg gcgtggccgc catcgtgttc gctttcttgg
     3361 agccgacact tcaggcccgg cgctcacagc cggccctggt ggtctcggtg gggatggccg
     3421 tcgacgtgtt cgtgatcagt ctcttgatcc tcgtccccat cgtcatgtcc ctgatgggtc
     3481 aggtcattcg ggagcgtggc cgaaccgaga gtctcctgct gaatgtgctt ccagagacga
     3541 tcgccggtcg cctcaagcta tctccgggcg tcatcgccga cgactatgag ggctgcaccg
     3601 tgatgttcgc cgacctggtc gggttcaccg accacacgag tcgcatctct cccgaacagt
     3661 tgatcactga gctcaatctg atcttctccc gcttcgatgc cctggtgcat gacctgggag
     3721 ccgagaagat caagaccatc ggcgacggct atctcgccgt tgcgggtgcc ccaattcggc
     3781 gtcccgacca tgccgagctg atgtgcgagc tggcattgcg catgcaagaa accatgcccg
     3841 agatcaacga agagctgggg tcatcgctcc agcttcgcat cgggctccac accggcccgc
     3901 tcgtcgccgg tgtggtcggg gtctcacgct tcgcctatga cctgtggggt cacacggtga
     3961 acctggcaag tcgcatggag accgtgagcc caccaggcgg catccgcgcc accgacgccg
     4021 tcgcccgcgc cgccggggac cgctatgtct tcgagcagca cggcttcagc gatgtcaaag
     4081 gcgtcggtcc ggtggcaacg tgtctcctgg tcggacgcaa gcaggtgcag gtggaacagc
     4141 cagccctagc ccacccagag accctgagct gacctgacat
//
LOCUS       DASUTA010000192         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1740506, whole genome shotgun sequence.
ACCESSION   DASUTA010000192 DASUTA010000000
VERSION     DASUTA010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740506"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..2103)
                     /locus_tag="VFZ80_03825"
     CDS             complement(103..2103)
                     /locus_tag="VFZ80_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6286591.1"
                     /translation="MDPNTPDLTRIFREELDERTRRLVDAARSLEKGELASDAVEGIL
                     RDAHTIKGSAALLGHELIRDGARKLEQLWREVIDSSSVDPGKVVAMEAIAGRLTGALD
                     DPDDPELRRLIEDAGNAQIPHAPELVSLRPPEVGSLGGLLTSVSESLLGGATRVDTAD
                     LYRLINRIVEVSLDAEAIADLSLVSIEGVDPDVFRRSWRIQLEKLSDSVADIQAQAVS
                     LANVSFFDAVSTFPQFVRYLGRRSGKDVRLELTGHDVQLDRQIVDLLREPLRHLLVNA
                     VDHGIEPSDERLAAGKPGTGVIRVAARVVDDGVEVTVSDDGAGVDWSRVADVARQKGL
                     PFGSSDLAPLLFHPGFSTLAEATEFSGAGEGLTEAAEAATQVHGAVSLDSEPGKGTTV
                     TMTLPLSMVLQNVVVVAEGDLFWGIPEASVEGSMPLANAEFTTESNGREVRFESAAVP
                     CLSLSEAVGGDEPSDTSEILVINTRSGLVAVTVSEIIDRRRVAVKNLGPIIEGSGGTK
                     GAALLGGGQVLVVLDPDFLGVLARHRPHLVSSKPRVLVVDDSAGVRQLLSATLTGAGF
                     EVEVASSAREAMLAMTRDGFSAMVVDYSMPRSNGVELVRSMRAADVRIPIVMVSGVAT
                     QEEKDAAWAVGVDAYLDKFDLRQGVLTKTLRQLIGLEEADTA"
BASE COUNT          409 a          738 c          663 g          369 t
ORIGIN      
        1 ctccggccca gagaagacac cgttgcccgt tcgaccacat acccagggtt gcagactccg
       61 ctcagcgaag tctccggccc ggagaagaca gaagctcttc tctcaggcgg tatcggcctc
      121 ctcgagacct atcaattggc gaagggtctt ggtgaggaca ccctgacgaa gatcgaactt
      181 gtccagatag gcgtccactc ccaccgccca tgccgcgtct ttctcctcct gggtggcaac
      241 cccggagacc atcacgatgg gtatgcgcac atctgccgcc cgcatcgagc ggaccagttc
      301 gacgccgttg gagcggggca tcgagtagtc gacgaccatg gcgctgaacc cgtcgcgggt
      361 catcgccagc atcgcttcgc gagcgctgct cgccacctcg acctcgaacc cggctccggt
      421 cagggttgcc gagagcaatt gacggacccc agcggagtcg tcgacaacga gcacccgtgg
      481 cttgctgctg accagatgcg gtcgatgacg ggcgagaacg cccagaaaat cggggtcgag
      541 gaccaccagg acctgcccgc cgccgagcag ggcagcgccc ttggtcccgc cgctcccctc
      601 gatgatcggg cccaggttct tcaccgcgac acgacgacga tcgatgatct cactgaccgt
      661 cacagccacc aacccggatc gggtgttgat cacgaggatc tccgaggtat cggacggctc
      721 gtcgcctccg accgcctcgg acagcgacaa acagggcacc gccgccgact caaaacggac
      781 ttcgcgtccg ttgctctcgg tggtgaactc ggcgttggcc aagggcatgg agccctcgac
      841 agacgcttcg ggtatccccc agaacaggtc tccctccgcc acgaccacca cattctggag
      901 aaccatcgag aggggcaggg tcatcgtcac cgtcgttcct ttgcctggct ccgagtcgag
      961 gctgaccgca ccatggacct gggtcgccgc ctcggccgcc tcggtgagac cttcgccggc
     1021 gccggagaac tcggtcgcct cggcgagggt gctgaagccg gggtggaaca acaaaggtgc
     1081 caggtcgctc gagccgaagg gaaggccctt ctgccgggcc acgtctgcca cccgactcca
     1141 gtccacgccg gcaccatcgt cggagacggt gacctcgacg ccgtcgtcga cgacccgcgc
     1201 cgccacccga atgacgccgg tccccggctt ccccgcggcg agtctctcat cagaaggctc
     1261 tattccgtgg tcgaccgcgt tgaccaggag atgacgtaga ggctcacgga gcagatcgac
     1321 gatctgacga tcgagctgca catcgtgacc ggtgagctcc agccgaacgt ccttcccgga
     1381 gcgacgtccc agatacctga cgaattgcgg gaacgtggag acggcgtcga agaacgagac
     1441 gttggccagg gacaccgcct gtgcctggat gtcggccacg gagtcactca gcttctccag
     1501 ttggatcctc caggaccggc ggaagacgtc ggggtcgact ccctcgatcg ataccagcga
     1561 gaggtcggct attgcctcgg cgtcgaggga cacctcgacg atgcggttga tgagtcggta
     1621 gaggtcggct gtgtcgaccc tggtcgctcc gccgagcagc gactctgaca ccgatgtcag
     1681 cagaccaccg aggctgccga cctcgggtgg tcgcaaggaa accaattcgg gcgcatgagg
     1741 gatctgggca ttgccggcgt cctcgatgag acgcctcaac tctggatcgt cgggatcatc
     1801 caaggcgcca gtcaggcggc cggcgatcgc ctccatggcc accacctttc ccggatcgac
     1861 ggaactggag tcgatcacct ctcgccatag ttgctccagc ttgcgggccc catctcgaat
     1921 cagctcatgg ccgagcagcg ctgccgagcc tttgatcgtg tgagcgtcgc gcagaatgcc
     1981 ctccacagcg tcgctggcca actcgccctt ttccaatgaa cgcgcggcat cgaccagacg
     2041 acgtgtgcgt tcgtccagct cctcccggaa tatccgggtg agatcagggg tgtttggatc
     2101 caatttgggg tgtctccacg ccgacgaagt ctctatcggc accgatggga cccgccctaa
     2161 gacactcacc ccaggttaa
//
LOCUS       DASUTA010000193         5567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_316979, whole genome shotgun sequence.
ACCESSION   DASUTA010000193 DASUTA010000000
VERSION     DASUTA010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316979"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VFZ80_03830"
     CDS             <1..544
                     /locus_tag="VFZ80_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6286592.1"
                     /translation="AADLAEATVSLAAEPGRLIFTYVPFVDFAGHVFGQGSDEFAEAM
                     RLATAVWDGIVSALPPGAAVVATADHGLMEIGESDKLLVREPEFDALRLAGDPRGVQV
                     WGEPRLIEDLAAFTGGALIDPAPLLGPDLSTDARSRLGEKVLLAPPGKVILPRGFDKR
                     LRGYHGGLSDEEVKIPLLVG"
     gene            complement(594..1094)
                     /locus_tag="VFZ80_03835"
     CDS             complement(594..1094)
                     /locus_tag="VFZ80_03835"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX6286593.1"
                     /translation="MTSNRNRLEACLEDALLASKVTVSELRTVLAREGCSPGTAVLDH
                     LVTEHSEDAPTPDSTYLEALLERLLRRAGTPEWHREFPFSIRGAPARVDVFIQDWDLV
                     IEADGRPWHGRMRDLEADKRRDAELAGRGIQVIRFTYSMLTDEPEECLGTIRGAGKHR
                     SAPRSV"
     gene            complement(1630..2124)
                     /locus_tag="VFZ80_03840"
     CDS             complement(1630..2124)
                     /locus_tag="VFZ80_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020517758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HEX6286594.1"
                     /translation="MMHQGRFTADFDGDFVVFLIGSRVHKPFRFRQWLPVARAMTTMQ
                     REIAEHPEIGCLHIENFGAMRNISVQYWRSFEDLERFARSGEWFHLEAWRQFNKLIRD
                     SGDLGIWHETYMVRAGEFETMYGNMPRFGLAAAGRHLPLTPSSTAARRAGRRPADTAP
                     VTGY"
     gene            2186..2782
                     /locus_tag="VFZ80_03845"
     CDS             2186..2782
                     /locus_tag="VFZ80_03845"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WHG domain-containing protein"
                     /protein_id="HEX6286595.1"
                     /translation="MSPAQTDTYHHGDLRRALIDEAGKTLEKAGVDAISFRGLARALG
                     VSHAAPGHHFADRNELLSELAADGYSGLAHALEAAMQDHSPDTWLERTGMAYVRFALN
                     NPERYRMMFASRLMTEDCPDRLRVESSRAYLLLLEAAYGGVPEIDPAVYRLQTAELRA
                     WSLVHGAVMLWLDGQLGPDVDEATFLTLTEEMLSETFA"
     gene            2841..4778
                     /gene="glgP"
                     /locus_tag="VFZ80_03850"
     CDS             2841..4778
                     /gene="glgP"
                     /locus_tag="VFZ80_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX6286596.1"
                     /translation="MSPDEIRDDLRRLAANYRWTWSPPSRQLLDRLPGAHSLRHPVAV
                     VDGLTADQLDALTTDDGLVRRLAEELDALDSLLDDRPEPTIAYCSPEFGLSALLPQYA
                     GGLGVLAGDHLKTASDLRTPLVGVGLFYSEGVFRQVIEEGQQTEAYHPIDPVGVGAAD
                     TGLVVHVPFPGRDVVAKVWRVEVGRVPLLLLDTNVDENSDHDRLITDRLYTGDRAHRL
                     EQEMVLGVGGARALEAMGWDIELHHLNEGHAGFIVLELIDRINGDTPLEEARARVREG
                     LVFTTHTPVPAGIDRFHSETIRPYLEVWAKRWDRAMSELWEMGADPDDTSMFNMAALC
                     LRSARTANGVSELHGQVSRKLFAGVGIGDDIGFVTNGVHSRTWTAPHTQEMFDRTLGE
                     SWADGDQAAWDGVTAIDDEMLLANRRLSSQVLADLVTDRTGDFIDPDALIIGFARRFA
                     PYKRASLFLRRPDLLEAIMHDDHRPVHFVFAGKAHPSDQTGKDLVADLIEATGVDLCN
                     GRLTFIPDYEMDIAMAMVQGADVWLNNPIRPREASGTSGEKAVLNGSLNCSILDGWWA
                     EMYDGRNGWEIQASPSPDEPTRDLEEATAMLETLTAIATEYHESRSIFLGRIRHSWLS
                     IGARVTAARMLRDYADRVYHT"
     gene            complement(4788..>5567)
                     /locus_tag="VFZ80_03855"
     CDS             complement(4788..>5567)
                     /locus_tag="VFZ80_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX6286597.1"
                     /translation="ELDGAAELMGAHLAEAELLGRRTGELHLELAADPTSHVFAPEPM
                     STLQQRSVYQAIRSTVRTSLSLLRRRRSKIETPESELIERLVAAEGELLEKLKSITAE
                     RIECDRIRIHGDYHLGQVLFTGNDFYIIDFEGEPQRPLSQRRLKRLALRDVAGMIRSY
                     HYAVVMAMRQVSEAGLDEDAVVTLQHWAEAVHGYLSAAFLDGYRQAVEGSSIVPENPT
                     HFRLLLDALIVEKAAYELEYEINNRPDWVGIPLQGILSILE"
BASE COUNT         1007 a         1791 c         1750 g         1019 t
ORIGIN      
        1 cgccgccgat ctcgctgagg ccactgtgtc tctggcggcg gagccgggcc ggctcatttt
       61 cacctatgtg ccgttcgtcg acttcgccgg acatgtcttc ggccagggtt ccgacgagtt
      121 cgccgaggcc atgaggctgg cgacggccgt ctgggatggc atcgtctccg ctctgccacc
      181 gggagcggca gtggtggcta cggccgacca cggactgatg gagataggcg agagcgacaa
      241 gctcttggtc cgcgaaccgg aattcgacgc gctacggctg gccggtgacc cgcgcggggt
      301 ccaggtgtgg ggcgagccga ggctgatcga ggacctggct gccttcaccg ggggggccct
      361 gatcgaccct gccccactgc tgggccccga tctgtcgacc gacgcccgat cccgcctcgg
      421 cgagaaggtc ctcctcgccc cgccgggcaa ggtgatcctg ccccgcggct tcgacaaacg
      481 cctccgcggc taccacggcg gcctcagcga cgaggaggtc aagatcccac tactggtggg
      541 gtgagggggt ggcgcttcct ctaattcgtt ttgcccgcgg gcaacacgtt gcgctagacg
      601 ctacgcggtg cactccgatg ctttcctgct ccccgaatcg tgcccaaaca ctcttctggc
      661 tcgtcggtca gcatcgagta cgtgaagcgg atcacctgaa ttccccgccc ggcgagctct
      721 gcgtcacgtc tcttgtcggc ttccaggtca cgcattcgcc cgtgccacgg gcgaccgtcg
      781 gcctcgatga ctagatccca atcttgaatg aacacgtcga ctcgagccgg agcacccctg
      841 atcgagaacg ggaattctcg gtgccactca ggggtcccgg cacgtcggag aagacgttcg
      901 agcaacgcct cgagatatgt cgagtctggt gtcggtgcgt cttcggagtg ctccgtgaca
      961 aggtggtcta gtaccgcggt gcccggtgag cacccctccc gagcgaggac cgtccgcagt
     1021 tccgagaccg ttactttgct cgcgagcagg gcgtcctcga ggcaagcctc gagacggttc
     1081 cgattcgagg tcacgggcca gcatcatcag agtttccgcc gcggacgtaa ccgtgaagcc
     1141 agcgatctgc tcggtcgcca acaggagaaa gtgcttcgat ctgatgacgc gaccgatcgt
     1201 caagcgggtg ttcgcgccgt tcggaaccac caccgtcggc tttcctcttc ggaatcccct
     1261 gaacccgagc agataggcag cggagcggcc agctacgtag gcgtcttggc ggctgagaac
     1321 cgccgccgct agctgtcgct cccactttgg tggtgccgac gccaacgcga acacgttcgg
     1381 acatattcgg atccactcgc cggactcgac ccgccgcccc accgcgaatc gatcgaaccc
     1441 ggcatccctg agctggactc tgttgaacac tccgtattgg cggcttgcaa cctggttgat
     1501 ctgatggata tcccccacaa gccgaccatc cgtgatgcca gcagcacatt caaccccctt
     1561 gaccgtgact tgagtgcaga gcgttgcgct cagcgcaacg ctctgcccgc gggcaggacg
     1621 agcaacgcgt tagtacccag tcacgggggc agtgtcggcg ggacgacggc cggcgcggcg
     1681 ggcggcggtg gacgaggggg tgagggggag atggcggccg gcggcggcga ggccgaagcg
     1741 gggcatgttg ccgtacatcg tctcgaactc gccggcgcgc accatgtagg tctcgtgcca
     1801 gatgccgaga tctcccgaat cacggatcag cttgttgaac tgacgccatg cctcgaggtg
     1861 gaaccactca ccggaacggg cgaaacgctc cagatcctcg aatgagcgcc agtactgcac
     1921 cgagatgttg cgcatggcac cgaagttctc gatgtggaga cacccgatct cggggtgctc
     1981 ggcgatctca cgctgcatgg tggtcatggc acgcgccacc ggcagccact ggcggaagcg
     2041 aaaaggcttg tgaacccggg agccgatcag aaatacgacg aaatccccgt cgaaatccgc
     2101 tgtgaatctg ccttggtgca tcatgagccg gtcctatctt gtcggtgtca agatgacgat
     2161 atgtgttaat cttggcgctg tcaagatgtc accagcgcag accgacacct atcaccacgg
     2221 cgatctccgc cgtgctctga tcgacgaggc cggaaagacc ttggagaaag cgggagtcga
     2281 cgccatctcg tttcgggggt tagccagggc gctgggtgtc tcacacgccg cacccggcca
     2341 ccatttcgcc gatcgcaacg agttgctctc tgagctcgcc gctgacggat actccggtct
     2401 ggcccacgcg ctcgaagccg ccatgcagga ccactcgccc gacacctggc tggagcggac
     2461 cggcatggcc tacgtcagat tcgccctcaa caacccggag cgataccgga tgatgttcgc
     2521 ctccagattg atgaccgaag actgcccaga tcggctgcga gtggagtcga gccgggccta
     2581 ccttctactc ctggaggcgg cctatggagg tgtgccggag atcgatccgg ccgtctatcg
     2641 gctgcagacc gcggagttga gggcctggtc gttggtccat ggagccgtga tgttgtggct
     2701 cgacggtcag ctcggtcccg acgtagacga agccacgttc ctcactctta ccgaagagat
     2761 gctttccgag acgtttgcct aaactccgga aacggccttc gatggagaga tcgaaggctt
     2821 ttctgcgtac aggagtgctc ttgagccctg atgaaatccg agatgatctg cggcgtctcg
     2881 ccgcgaacta ccgctggacc tggtctccac ccagtcggca gctactcgat cgtctgcccg
     2941 gcgcccattc gttgcgtcat cccgtggcag tggtcgacgg cctcacggcc gaccaactgg
     3001 atgcactgac gaccgacgac ggcttggttc gtcgattggc cgaggagctc gacgccctgg
     3061 acagcctctt ggatgaccgg cccgagccga cgattgccta ctgctcgccc gagttcggtt
     3121 tgagcgcgct tctcccccag tatgcgggcg ggctcggcgt cctcgccggt gatcacctca
     3181 agacggccag cgatctccgg acgcccctgg tcggggtggg gctgttctac tccgaagggg
     3241 tcttccgcca ggtgatcgaa gagggccagc agaccgaggc ctatcacccg atagaccctg
     3301 tcggagtcgg ggcggcagac accggtctgg tcgtccatgt ccccttcccc ggccgcgacg
     3361 tcgttgccaa ggtgtggcgc gtcgaagtcg ggcgcgttcc gctcctcctt ctcgacacga
     3421 acgtggatga gaactcggac cacgaccggc tcatcacgga ccggctgtac accggcgacc
     3481 gggcgcatcg cctggagcaa gaaatggtgc tcggcgtggg tggtgcgcgg gcgttggagg
     3541 cgatgggatg ggacatcgag cttcatcacc tcaacgaggg ccatgccggg ttcatcgtct
     3601 tggagctgat cgacaggatc aacggtgaca cgccgctcga agaggcccgc gcccgtgtcc
     3661 gcgagggact cgtcttcacc acccacaccc cggtgccggc aggaatcgac cgcttccact
     3721 ccgagaccat ccgtccttat ctcgaagtct gggcgaagcg atgggatcgc gccatgtccg
     3781 aactgtggga gatgggcgcc gatccggacg acacttctat gttcaacatg gcggcgctgt
     3841 gcctgcgaag tgccaggaca gcgaacggcg tctccgagct tcacggccag gtcagtcgca
     3901 aactcttcgc cggagtcggg atcggcgacg acatcggttt cgtcacgaac ggggtgcatt
     3961 ccaggacctg gaccgcccca cacacccagg agatgttcga caggacgctc ggcgaatcct
     4021 gggcggacgg agaccaggcg gcatgggatg gcgtgaccgc catcgacgac gagatgctcc
     4081 tcgccaatcg gcgcctgtcc tcgcaggtcc tggccgacct ggtcacggac cggacagggg
     4141 atttcatcga ccccgacgcg ctcatcatcg gcttcgcccg tcgtttcgcc ccctacaagc
     4201 gagcatcgct gttcctgaga cggccggatc tgctggaggc gatcatgcac gacgaccatc
     4261 gaccggtgca cttcgtgttc gccggcaagg cccatccgtc ggatcaaacg ggaaaggatc
     4321 tcgtcgccga tctcatcgag gcaaccggcg tcgacctgtg caacggacga ctcacgttca
     4381 tccccgacta cgagatggac atagcgatgg cgatggtcca aggtgccgac gtctggctca
     4441 acaacccgat tcggccacgt gaggcctcgg gtacgagtgg agagaaggcc gtcctgaacg
     4501 gctccctgaa ctgctcgatt ctcgacggat ggtgggccga gatgtacgac gggcgcaacg
     4561 gctgggagat ccaggcgagc ccttcacccg acgagcccac cagggatctc gaagaagcca
     4621 cagcgatgct ggagacgctc accgccatcg ctaccgagta tcacgagtcc cgatcgatct
     4681 ttctgggcag gatcaggcac tcatggcttt ccatcggtgc tcgagtcacc gcggcgagga
     4741 tgctccgtga ctacgccgat cgtgtctacc acacctgatc accggcgtta ctcgaggatg
     4801 ctcaggatcc cttggagtgg gatgcctacc cagtcgggcc ggttgttgat ctcatactcc
     4861 agctcgtagg cggccttctc cacgatcagg gcgtcgagaa ggagccggaa gtgtgtggga
     4921 ttctccggaa cgatgctcga gccctcgact gcctgtcgat acccgtcgag gaacgcagcg
     4981 gacaggtagc cgtgtacggc ctcagcccaa tgctgcaggg tgaccactgc gtcctcatcc
     5041 aggcccgcct cggagacttg cctcatcgcc atcaccaccg cgtagtggta agagcggatc
     5101 atcccggcga catcacggag cgccaaacgc ttcagacgtc gttggctcag tggccgctgc
     5161 ggctccccct cgaagtcgat gatgtagaag tcgttgccgg tgaacaggac ctggccgagg
     5221 tgatagtcgc cgtggattcg gatgcggtca cattcgatcc gctccgctgt gatcgacttc
     5281 agcttctcga gcaactcccc ctccgccgcc accaaccgtt cgatgagctc tgactcgggc
     5341 gtctcgatct tcgatcgcct cctgcgaagc aaggagaggc ttgtcctgac cgttgaacga
     5401 atggcctggt agaccgacct ctgctggagt gtggacatcg gctccggggc gaacacgtga
     5461 ctcgttgggt cagcggcgag ctcgagatgg agctcaccgg tgcgacggcc aagaagctct
     5521 gcttcggcga gatgagcacc catcaactcg gccgcaccat ccaactc
//
LOCUS       DASUTA010000194         2265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1094145, whole genome shotgun sequence.
ACCESSION   DASUTA010000194 DASUTA010000000
VERSION     DASUTA010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094145"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            513..1256
                     /locus_tag="VFZ80_03860"
     CDS             513..1256
                     /locus_tag="VFZ80_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019434519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6286598.1"
                     /translation="MALDCAVITGAGSGIGAATAILLAERGMKVVGVDVDEGTSQTVT
                     ESIRSAGGSAWSYQGDVADEEMWAGLVSWLQRNVGPVRHFVSNAVIIDRDAAGDLSRD
                     RWDRQIAVNLTASFLGFRALVDDLKITRGSAVLVSSVQAMVGIPGHPAYAAAKAAMIG
                     LTRQLGVDYGPEVRTNAVLPGPILTGVWDVIPQEDVERSAAATVAGRLGRPSEVAQAI
                     AFLLSDEASYVTGASLVVDGGWTITKDSV"
     gene            1253..1540
                     /locus_tag="VFZ80_03865"
     CDS             1253..1540
                     /locus_tag="VFZ80_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286599.1"
                     /translation="MSRPRGADPINPGAISPASGNQLLIRIADLIEQTHAVHDLLIYL
                     PLVSREPVAIHRSPIGAFQFSRPEAAARISMELLSAASKDARHIRGEGAIR"
     gene            1537..>2265
                     /gene="dgoD"
                     /locus_tag="VFZ80_03870"
     CDS             1537..>2265
                     /gene="dgoD"
                     /locus_tag="VFZ80_03870"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HEX6286600.1"
                     /translation="MKIARIETFLVAPRWLFCRVETEDGLVGWGEPLLEGRAETVRTA
                     VGELTEILVGRDPRAIEDAWQIMTRGGFYRGGPILSSAVAGLDQALWDIAGKAAGLPV
                     YSLLGGPVRDRMRVYSWVGGDDPLGQEDAVLGEEVEQRLAAGFTAIKTNGIGPVGSPA
                     SRSQLRAAVDRIHKLREDFPSLDIGVDFHARVAISDAVQLVSRLDDAELLFIEEPTRP
                     EDVSQIGRVVSATTTPIALGERLYS"
BASE COUNT          439 a          683 c          711 g          432 t
ORIGIN      
        1 cgagcgtccg acggcccgct cgaatgacgc accctcgaca ttcatgatca cgtcgactgc
       61 accaacgccc gcgccccaaa aggcgaaccc gagggacacc aacagatacg atcgaagacc
      121 ctgggcccct attccaatca aggctatacc cgctgagaac tgcaacatcc cccagaccgt
      181 gccccggcgc gccccgagtc gcaccagtaa gtatgacccc gccgaaagcc cgacgatgga
      241 agccaacccg ccagcaaaca gcaggaggcc tacctgactc tcggtcaccc caagcgcgtc
      301 tcgaattgcc ggcagacgtg aggcccagct agcaaggcca agtccgcagt tgaagtagat
      361 cgccatcatc gaccagcgcc aacggctgag acgagcccct gtgccgaccg gctctgtagt
      421 gttcgccgag ggcgtttttt ggagtcctgg atttgagtaa tcgatttctg aactataaag
      481 gcgacaacca acacggaaac taggaatgtg agatggcctt ggactgtgcg gtgattactg
      541 gtgccggttc cgggatcggc gcggcgacgg cgattcttct tgccgaacga ggtatgaagg
      601 ttgtcggtgt cgatgtcgat gaagggacga gccagaccgt gaccgaatcg atccgatcgg
      661 ccggggggtc agcatggagc taccagggtg atgtggcaga cgaggagatg tgggccggcc
      721 tggtttcatg gctccagcgg aatgtcggtc cggtacgtca cttcgtcagt aatgcggtga
      781 tcatcgaccg cgatgctgcg ggagatctga gcagagatcg ctgggatcga cagatcgcgg
      841 tcaatttgac cgcttccttc ctcggatttc gcgctctggt cgatgacttg aagatcacgc
      901 gggggtcggc cgtgctggtg tcttcggtgc aggccatggt cgggattccc ggccatcccg
      961 cctacgccgc cgccaaagcc gccatgatcg gtctgacccg acaactcgga gtcgactacg
     1021 gacctgaggt acgcaccaat gcggtgttgc ccgggcctat cttgaccggg gtgtgggacg
     1081 tgattcccca ggaggatgtc gagcgcagcg cggcagcaac agtggccggt cgactgggac
     1141 gaccttcaga agtagcccag gccatcgcgt tcctgctttc cgatgaagcc tcgtacgtca
     1201 ccggggcctc actcgtggtc gacgggggtt ggacaatcac caaggattcg gtgtgagtcg
     1261 tccgagagga gctgacccca tcaaccccgg cgccatttcc ccggcctcgg gtaaccagct
     1321 tctcatccga atcgccgact tgatcgaaca gacccacgct gtccacgatc tactcatcta
     1381 tctcccattg gtgtctcgcg aaccggtggc gatccatcgc tcccctatcg gtgccttcca
     1441 attctctcgg cccgaagcgg ccgcccggat atccatggag ttgctgtcgg cggcatcgaa
     1501 ggatgcccga cacatccgcg gcgaaggggc gatccggtga agatcgcccg tatcgagacc
     1561 ttcttggtag cgccacgctg gctcttctgc cgtgtcgaga cagaggacgg gttggtcggc
     1621 tggggcgaac ctttgctcga gggtcgcgcc gaaacggtga gaactgcggt cggcgaactc
     1681 accgaaatcc tcgttggcag ggatccgaga gccatcgaag acgcctggca gatcatgacc
     1741 cgaggcggct tctatcgggg tggtccgatt ctctctagtg ccgtcgccgg attggaccag
     1801 gcactttggg atatcgcggg caaggcagcg ggacttccgg tctattccct tctgggtggc
     1861 ccggtgcgcg accgcatgag ggtgtactcc tgggttggtg gcgatgatcc actcggtcaa
     1921 gaggacgctg tgttgggcga ggaggtcgag caacgccttg ccgccgggtt caccgcgata
     1981 aagaccaacg gcataggtcc ggtaggttct cccgcctcgc ggtcccaact cagagcagcg
     2041 gtggaccgta tccacaagct gagagaagac tttccgtcgc tcgacatcgg tgtggacttc
     2101 catgcgagag tggcgatctc cgatgccgtg caattggtgt cgaggctcga cgacgccgag
     2161 ctcttgttca tcgaggaacc tacccgtccc gaggatgtct cccagatcgg gagggtcgtg
     2221 tcggcgacaa ccacaccgat cgcgctgggg gagcggttgt attca
//
LOCUS       DASUTA010000195         2467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1354715, whole genome shotgun sequence.
ACCESSION   DASUTA010000195 DASUTA010000000
VERSION     DASUTA010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354715"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFZ80_03875"
     CDS             complement(<1..180)
                     /locus_tag="VFZ80_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286601.1"
                     /translation="MGLIESIVFIGLGIAVIWIGITLIRQGHPEDDVERDLPENPGPT
                     GEAYPTGSRPAGPGAE"
     gene            complement(226..1014)
                     /locus_tag="VFZ80_03880"
     CDS             complement(226..1014)
                     /locus_tag="VFZ80_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6286602.1"
                     /translation="MQLDIDGETVFAATGGQPIEPDKQTVLFIHGVGQDHTIWVLPIR
                     YFARHDRNVLAVDLPGHGLSSGAPLPTIEAMADWVSEVVAAAGVERAALVGHSMGSLV
                     ALDAAARHPDKVRAVVMIGVSVPMRVADTLMSGAEANDQVAVDMLTYWGHSDGAKLGG
                     SPTPGIWMPGASSRLWERAAPGVLHTDLRACESYDSGLERAAQLRCPALLLLGEKDRM
                     TPVRGAEAIREAIPDQNTIVFPGAGHPLLLERSDQVLDELIEMV"
     gene            complement(1014..2309)
                     /locus_tag="VFZ80_03885"
     CDS             complement(1014..2309)
                     /locus_tag="VFZ80_03885"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEX6286603.1"
                     /translation="MAKPEPYDPDTLALHAGQHPDSDFGARAVPIYQTAAFVFPDADT
                     AASRFNIERTGHVYSRLTNPTNAVLEERISALEGGVGAIATSSGQAATHLGIATLMGA
                     GSHIVSSQSIYGGSHNLLAYTLPRFGIETTFVDPRDPAAFSAAIRPNTRLIFGETIGN
                     PGMEVFDVPTIAEIAHQHGVPLMIDSTFTTPVLARPFDMGADIVVHSATKFLSGHGVV
                     IGGLLVDAGAFDWQGSGKFPTMTEPYEGFHNLVFHEEFGPTAFITRARKEGARDFGPA
                     MAPMTAFLILQGIETLGLRMRRHVENAMAVAKFLEDHEAVEWVRYPGLESHPDYELAR
                     RLLPRGAGSIFSFGIRGGRAAGQRFIENVVIASHLANVGDAKTLVIHPASTTHHRMDS
                     KALEEAGISEGLIRISVGLEGIDDLIEDLGRALHASQRL"
BASE COUNT          443 a          773 c          794 g          457 t
ORIGIN      
        1 ctcggcacca ggccccgcag gtctggatcc agtcggatag gcctctccgg tcggtcccgg
       61 gttctcgggt aagtctcgct ccacatcgtc ttcggggtga ccttgacgga tcagtgtgat
      121 cccgatccag atcacggcta tcccgagccc gatgaacacg atcgactcga ttagacccat
      181 gaaccaccca ttaccaaatc ggcgatccgg gaaaccgaaa ccgattcaca ccatctcaat
      241 cagctcatcg agcacctggt cggagcgctc gagcagcaga gggtgacctg ctccgggaaa
      301 cacgatggtg ttctggtccg ggatggcctc tcgaatggct tctgctcctc gcacgggcgt
      361 catgcgatct ttctccccga gcagcaggag tgccgggcat ctcagctggg cagcgcgctc
      421 cagaccggag tcgtagctct cgcaggcccg cagatcggtg tggagaaccc ctggtgcagc
      481 tcgctcccag agcctggagc tggccccggg catccatatg ccgggagttg ggctaccccc
      541 tagcttggcg ccgtcgctgt ggccccagta agtgagcatg tcgactgcga cctggtcgtt
      601 ggcctcggcc ccagacatca aagtgtcggc tacccgcata gggaccgaga ctccgatcat
      661 gacgactgct ctcaccttgt ctggatgacg ggcggccgca tcgagagcga ccagcgagcc
      721 catgctgtgt ccgaccagcg ccgcccgctc caccccggct gccgcgacaa cctcgctcac
      781 ccagtcggcc atggcttcga tggtgggcag aggggcacca gaggaaagcc cgtggccggg
      841 gaggtcaacg gcgagcacgt tgcggtcgtg gcgggcgaag tagcggatgg ggaggaccca
      901 tatggtgtgg tcctggccca caccgtggat gaacaagacg gtctgtttgt cgggttcgat
      961 cggctgaccg ccggtggcgg caaagacggt ctccccgtcg atatcgagct gcatcacagc
     1021 ctttgcgacg cgtgaagggc cctccccagg tcttcgatga gatcatcgat tccctccagc
     1081 ccaaccgaga tcctgatcag gccctcggag atgcccgctt cctccaaggc tttcgaatcc
     1141 atccggtgat gagtcgtgct cgccgggtgt atcaccaacg tcttggcgtc tccgacattg
     1201 gcgaggtggg acgcgatgac cacgttttcg atgaaacgct ggcctgccgc tcgtccgccg
     1261 cgaataccga agctgaagat cgaacccgca cctctcggca gcagtctccg ggccagctcg
     1321 tagtcggggt gagactccag cccgggatac ctgacccatt ccaccgcttc atgatcctcg
     1381 aggaacttgg cgacggccat cgcgttctcg acatgacgtc gcatgcgtag gcccagggtc
     1441 tcgattccct gaaggatgag gaacgcggtc attggcgcca tggccgggcc gaagtcccga
     1501 gcaccctctt tgcgggccct ggtgatgaag gcggtggggc cgaactcttc gtgaaagacg
     1561 aggttgtgaa aaccttcgta gggctcggtc atggtgggaa acttgcccga gccctgccag
     1621 tcgaaggcgc ctgcgtccac cagaagcccg ccgatgacga ccccgtgccc gctgaggaac
     1681 ttggtcgcgg aatgcacgac gatgtctgca cccatgtcga atggccgggc gagaaccggt
     1741 gtggtgaacg tcgagtcgat catcaacggg acaccgtgtt ggtgggcgat ttcagcgatc
     1801 gtcgggacgt caaagacctc catacccggg ttcccgatgg tttccccgaa gatgagcctg
     1861 gtattgggcc gtatcgcagc tgagaaggcg gccgggtcgc gggggtcgac aaacgtggtc
     1921 tcgatgccga accggggcaa ggtgtaagcc aacaggttgt ggctgcctcc gtagatcgac
     1981 tgtgacgaga cgatgtgcga tccggcgccc atgagagtgg ctatgccgag atgagtcgcg
     2041 gcctgaccgc tggacgtggc aatcgcccca actccccctt ccaaagcgga gatccgctct
     2101 tccaacaccg cattggtcgg gttggtgagc cgggagtaca cgtgaccggt gcgttcgatg
     2161 ttgaaccgag aggcggcggt gtccgcatcc gggaagacga acgcggccgt ctggtagatg
     2221 ggcactgccc gtgcaccgaa gtcggagtcg ggatgctgtc cggcgtggag ggcgagagtg
     2281 tccggatcgt acggctcggg cttggccatt ccccgtacgc tagagcagta gccgcgacgt
     2341 cgagatcgct gcaatcgaag gggcccctga tcccggggcc cctccacgct cgtcgcttac
     2401 tcgtaatccc gtcttggcgg gctggcaggc cggctgctcg tggcagcctc ctcctgggtc
     2461 tccttga
//
LOCUS       DASUTA010000196         4165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1743103, whole genome shotgun sequence.
ACCESSION   DASUTA010000196 DASUTA010000000
VERSION     DASUTA010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743103"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1485
                     /locus_tag="VFZ80_03890"
     CDS             226..1485
                     /locus_tag="VFZ80_03890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6286604.1"
                     /translation="MTEREDELWEYIRSLQNQIDRLVDTDEKRRSRIDKLEEDRRILR
                     ERLERARRRWRLRRSAPAATTEPRSRTGPPSPSLEVPGVGVIGESEIRDGAPRRDLKV
                     ATVLDPISATVFGPEFHHYPVSARSWQADIEEARPDLLFVESAYRGHDGNWATRVARF
                     GAPSHHLADLVAWCRTHDIPTVFWNKEDPINFDWFVESARLFDFVFTVDGDMIPRYRE
                     VLGHDRVQLLQFFAQPAIHYPGPEEQRKGAVAFAGSYFAFKHPERREQMEMLLDPARE
                     FGLHIFDRHGDTDDERFRWPEKYRPHIMGSLTYLQTVEAYRRYKVFVNVNTVTNSPTM
                     CARRVFELAACGTPILSGPARALAGVSEDGVVEVVSDQESTSRSLNRMLSDVELRHRT
                     DRTGRRWIDSGHTAAARVEKIVSLVGG"
     gene            complement(1482..2549)
                     /gene="wecB"
                     /locus_tag="VFZ80_03895"
     CDS             complement(1482..2549)
                     /gene="wecB"
                     /locus_tag="VFZ80_03895"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017618789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX6286605.1"
                     /translation="MQSLHVVGARPNFMKAAPVVAALVERGSRPYLVHTGQHYDEAMS
                     KIFFGQLGMPEPDRNLGIGSGSHAVQTAALMTALEDLFLELDPYRVVVYGDVNSTLAA
                     ALVASKLEIPVAHVEAGLRSFDRSMPEEINRIVTDSVSDLHFVTSPEALGHLAREGIA
                     DGSMHFVGNPMIDTLMRFRDHLDVDGVREAFGLPIPYAVVTLHRPSSVDDPEAVERVV
                     EGLRRASRHIDLIVPLHPRGREQLERAGLGEFARVVEPLGYLDFMALVMGSRVVLTDS
                     GGIQEETTVLGIPCLTLRENTERPITITMGTNRLVGTDPDAIEKGVVDVSSWPRGETP
                     PLWDGRAGKRIAEILLSTVPD"
     gene            2726..>4165
                     /locus_tag="VFZ80_03900"
     CDS             2726..>4165
                     /locus_tag="VFZ80_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX6286606.1"
                     /translation="MEDPSLRQRLLEITVLSSFAVGQPLLWLVVDNPTFAVVHGLTGF
                     QLIAFGIAVVALPALALGLLASLLAVGPRPLSVWSHRLIVGVLLAAIVTPAINRALGF
                     DHWWALVAFALVSTLGILAYTRLATLRTLIRYAVIAPLLLPVLFSFSGPIRPLVRATP
                     VDLPPISAASAPPVVWLVFDQLPLALLLDSEGEIAEDRFPNFARLRSLGTWYPKATSI
                     TPNTDTSMTATLTGLYPTPGAPSVTAQYPANAFTLLGGLYEVKSHERVTGLCPETICE
                     SVEDRQPPRTRLFQDSAIVVGRLLLPDGVADLFLPEVDSQWGEFGAAGSGGGSAPRVY
                     REDRAEFLSFVEDLGSSDAPAFHYFHMEKPHEPLLMIPDGRIYDYCSCYYTEEGGRWP
                     DDEVMLAQRLQRYVMQTMYVDHELGLVLDRLERLGTLDETLILVMSDHGASMIPGNVN
                     RSVDPETADEVFSVPLFIKYPNQQSGTVDL"
BASE COUNT          779 a         1372 c         1254 g          760 t
ORIGIN      
        1 cgctgtggaa cccgtcgatc gggaacacga ggggatggat ctcggcggca agggccggca
       61 gcatcaccgc tccgacgagg agacatgaca ggatgagaac gcgtctggca aagctggtca
      121 tgtagatgac caatcggtag ggagcagggt gaccttaacg aagatgccgg agcgaggtcc
      181 aaacgaccgt cgcctaccat caccgccgat gagagcggat gagccgtgac ggaacgtgag
      241 gacgagttgt gggagtacat ccgctccctc cagaaccaga tcgaccgact cgtggatacc
      301 gacgagaagc gacggtcccg aatcgacaaa ctggaggaag accggcgcat cctgcgggaa
      361 cgtctggaac gggcccggag gcgatggcga ctgagacgat cggcaccggc agcgactacc
      421 gaacctaggt cacgtaccgg ccccccgtca cccagtctcg aagtgcccgg ggtcggcgtg
      481 atcggcgagt ccgaaatcag ggatggtgcc ccgagacggg acctgaaggt ggccaccgtg
      541 ctcgacccca taagcgccac ggtgttcgga cccgagttcc atcactatcc ggtctccgcc
      601 cgctcgtggc aggccgacat cgaagaggcc cggcccgacc tgcttttcgt cgaaagcgcc
      661 tatcggggtc acgacggcaa ctgggcgacc cgcgtcgccc gtttcggcgc cccttcacat
      721 catctggccg acctggtggc ttggtgccgg acccacgaca tcccgacggt cttctggaac
      781 aaagaggacc cgatcaactt cgactggttc gtcgagtcgg cccgcctctt cgacttcgtg
      841 ttcacggtgg acggcgacat gatcccccgc taccgggagg tcctgggaca cgaccgggtc
      901 cagctactcc agttcttcgc ccagcccgcc atccactacc ccggacccga ggagcagagg
      961 aagggggcgg tggccttcgc cggttcctac ttcgccttca agcaccccga gcggcgggaa
     1021 cagatggaga tgctgctgga cccggcccga gagttcgggc tccacatctt cgaccgccac
     1081 ggcgacaccg acgacgagcg cttccgctgg cccgagaagt accggcccca catcatgggc
     1141 agcctcacct acctgcagac cgtggaggcc taccggcgct acaaggtctt cgtcaacgtc
     1201 aacaccgtga cgaactctcc caccatgtgc gcccgccggg tgttcgaact ggcggcgtgc
     1261 ggcacgccaa tcctgtcggg acccgcgcgt gcgctggccg gtgtctccga ggacggggtc
     1321 gtggaggtcg tgagtgacca agagtcgacg tcacgctcac tgaaccggat gctcagcgac
     1381 gtcgagctcc ggcatcgaac tgaccgcacc gggcgtcgct ggatagattc cggccacacg
     1441 gccgcggccc gtgtggagaa gatcgtctcc ctggtcgggg gctagtccgg aaccgtggag
     1501 agcaggatct ctgcgatccg tttgcctgcc cggccgtccc acaacggggg agtctccccc
     1561 cggggccacg aggagacgtc cacgactccc ttttcgatcg cgtccggatc ggtacccacc
     1621 aacctattgg tgcccatcgt tatcgtgatc ggtcgctcag tgttctcgcg aagagtcaga
     1681 cagggtatgc cgaggacggt ggtctcctcc tggatccctc cagagtctgt caggacgaca
     1741 cgggagccca tcaccagggc catgaaatcg agataaccca gaggctccac gacacgtgcg
     1801 aactcaccca agccggctcg ctccagttgt tcccttcctc ggggatgcag aggaacgatc
     1861 aggtcgatat gccgagaagc tcgccgtagc ccttcgacca cccgttcgac cgcctccggg
     1921 tcgtcgacac tggaaggacg gtgcagagtc accaccgcgt aggggatcgg caacccgaag
     1981 gcctcacgaa cgccatcgac gtccagatgg tcccgaaacc gcatcaatgt gtcgatcatc
     2041 ggatttccga cgaaatgcat cgaaccgtcg gcgattccct ctcgcgccag atggcccagc
     2101 gcctccgggc tcgtgacgaa atgcagatcg gatacggaat cggtcacgat ccggttgatc
     2161 tcctccggca tggaacggtc gaagctgcgc aaacctgcct cgacgtgggc gacgggtatc
     2221 tcgagtttcg aggccacgag cgccgctgcg agcgtcgagt tgacgtcccc atagacgact
     2281 acccggtagg gatccagttc gaggaacaga tcctccaggg cggtcatcaa ggccgccgtc
     2341 tggacggcat ggctaccaga accgatcccg agattcctat ctggctccgg catccccaac
     2401 tggccgaaga agatcttcga catcgcctcg tcgtagtgct gaccggtgtg aacgagatac
     2461 ggcctggaac ccctctcgac cagggcagcc accaccggtg ccgccttcat gaagttcgga
     2521 cgggccccga cgacgtggag agactgcatt gacacagtga gagggtaacg gttgtgtgtc
     2581 gaccgatctg acccatcgga tcccgtcgtg ctacgtttca cgtcctgtcc cgtcaagacg
     2641 agaggcgcga cgtttggatg actcgatccc ccattcgccg ggaaatgcga cgaccggctc
     2701 ggcgtcggcc gagactcacc ggagagtgga ggatccttcc ctccgtcaaa ggctcctcga
     2761 gataaccgtt ctctcttcgt tcgccgtggg ccaaccactc ctctggttgg tggtcgacaa
     2821 ccccacgttt gccgtcgtcc acggcctgac cggattccag ttgatcgcct tcggcatcgc
     2881 cgtagtggct ctgccggccc tcgctctcgg tctgctcgct tccctgttag cggtgggacc
     2941 gagacctctg agcgtgtggt cacaccgcct gatcgtgggc gtgcttctcg cggcaatcgt
     3001 tactcccgcc atcaaccggg cgctcgggtt cgatcattgg tgggccctcg ttgccttcgc
     3061 tctcgtctcg accctgggga tcctggcgta cacacgcctg gcaacgttgc gcaccctgat
     3121 tcgatacgca gtgatcgctc ctctcctgct cccggtcctg ttctcctttt caggtcccat
     3181 ccggcctctc gtccgggcga cgccggtcga tctccctccg attagcgccg catcggcacc
     3241 cccagtcgta tggctcgtct tcgatcaact ccctctcgcc ctcctgttgg attccgaagg
     3301 cgagatcgcc gaagaccggt ttccgaattt cgctcggctg cgcagcctcg gcacctggta
     3361 tccgaaggca acgagcatca ctccgaatac ggacacgtcg atgaccgcaa ctctcaccgg
     3421 cctctatccc actcccggtg cgccatcggt aaccgcccaa tatccggcga acgcgttcac
     3481 ccttctcgga ggcctctacg aggtcaaatc acacgagagg gtcacgggcc tgtgccctga
     3541 aaccatctgt gagtcggtcg aagatcggca accgcccagg acgcgcttgt ttcaggactc
     3601 cgccatcgtc gtggggcggc tgctgctccc agacggagtg gccgacctat tcctccctga
     3661 agtcgactcc cagtgggggg agttcggggc ggcaggttcg ggaggaggat cggcaccgag
     3721 ggtctaccgc gaagatcgag ccgaattcct ctcattcgtt gaagacctcg gcagttccga
     3781 tgcccccgcc ttccactact tccacatgga gaagccgcat gaaccactgc tgatgatccc
     3841 cgatgggcgg atctacgact actgctcctg ttattacaca gaggagggag gtcggtggcc
     3901 ggatgacgag gtcatgttgg cgcagcgcct gcagcgctac gtcatgcaga cgatgtacgt
     3961 cgatcacgag ttgggattgg tactggatcg cctggagagg ttgggaaccc tcgacgaaac
     4021 gctgatcctg gtgatgagcg atcacggcgc ctcgatgatt cccggaaacg tcaaccgcag
     4081 cgtggacccc gaaaccgcgg acgaggtgtt ctccgttccg ctgttcatca agtatccgaa
     4141 ccagcagtcc ggcaccgtgg atctt
//
LOCUS       DASUTA010000197         2247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1484299, whole genome shotgun sequence.
ACCESSION   DASUTA010000197 DASUTA010000000
VERSION     DASUTA010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484299"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..1071
                     /locus_tag="VFZ80_03905"
     CDS             370..1071
                     /locus_tag="VFZ80_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX6286607.1"
                     /translation="MKQIFDDRSKYLDRFGLLLIVTALAVITLSLIDIGESRDDLATS
                     VAQLIVSVFVGATLLLALLASGVRRRYQVTANLIIGAGVLASLVIVLLQAFGVSEPGQ
                     IGPRSVVWTVLSLLAPILVIRRLIRRRRATVQTLLGALSAYLLIALAFNFSFLVIDAF
                     GEPFFGSAEPTTSFMYFSLVTLTTLGYGDLAAAEPIGRLASTIEAVVGQVYLVTFVAM
                     IVGLLVAQAQGSEKG"
     gene            complement(1088..>2247)
                     /locus_tag="VFZ80_03910"
     CDS             complement(1088..>2247)
                     /locus_tag="VFZ80_03910"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX6286608.1"
                     /translation="LDGLLANTSIETLKSYLRWHLVRRFAPALPQVFEEEAFDFYNRK
                     LGGQQEPRERWTRVLGAATADIGELVSRLYVDEAFSPGAKERCEEMVDHLIEAMARSI
                     QGLEWMTDTTKKAALAKVRTFTHKIGYPDEWRDYSLLVVDRTSFVANRMRAATFEFDR
                     KMSRLEDPVDKGEWELPAHVVNAYYNPLHNEVVFPAGILQPPFFYADADDAVNYGGIG
                     AVIGHEITHGFDDNGSRFDEGGRRRNWWSDEDRVEFERRAQVLVEQYSDYGVFDDLKV
                     NGKLTLGENIADLGGLAVAYDAFSSTLDGAGPDLGGLSPLQRFFVSFATIWRMNYSDE
                     YLRMLTNVDVHSPNRYRANGPLSNFPPFAEAYGVDEGSPMRRPADRLVDIW"
BASE COUNT          401 a          719 c          711 g          416 t
ORIGIN      
        1 gagctcctcg gcgtcgccct ctccaggcac aacgacgagc aaggtctccc cgacactgtc
       61 gaagaggccc gcaattgcga acgcacgccc gctcgcaccc acagcccagg tcgacgcgag
      121 atcggggagg ggctgtgagc gccaagctga caagagcggt tgcaacggcg cgggcatcga
      181 gatcaacgat cgtactgacg cctcggcact gagaccgatc cttaggatgt cggggccatg
      241 agagggttca ccggccagtg atcgagctga ccgaggcggc gcagaccgtc gagttccgcc
      301 tgctcatagc ggcaggccca cccgcaagag gagcgaccga ccgctcacca cgatcacagg
      361 aggagcctgg tgaagcagat cttcgatgac cgttcgaaat acctcgaccg gttcgggctc
      421 ctgctgatcg tcaccgccct cgccgtgatc accctgtcgt tgatcgacat cggcgaatcc
      481 cgcgacgacc tggccacatc ggtagctcag ctcatcgtca gcgtcttcgt cggagccacc
      541 ctcctgctgg ctctcctcgc ctcgggagtt cggcgccgct atcaggtgac tgccaacctc
      601 atcatcggcg cgggggtgct cgcttcgctg gtgatcgtcc tcctgcaggc gtttggagtc
      661 agcgagccgg gccagatcgg gccgagatcg gtggtgtgga cggtgctgtc actgctggcc
      721 ccgatcctcg tgatcagaag gctgatccgc aggcgccggg cgacggtgca aacccttctc
      781 ggagccttat ctgcctatct gctgatcgcg ctggcattca acttctcgtt cctggtgatc
      841 gatgccttcg gcgagccttt cttcggctcc gccgagccca ccacttcgtt catgtacttc
      901 agcctcgtga cactgacgac actcggttac ggagacctgg ccgcggcgga gcccattggg
      961 cgcctggcgt ccacgatcga ggccgtggtg ggtcaggtct atctggtgac gttcgtcgcg
     1021 atgatcgtgg ggctcttggt ggcgcaggcc caaggaagcg aaaagggcta gcacccaggg
     1081 cagacattca ccagatgtcg accaggcggt cagccggccg gcgcatcggg gagccttcgt
     1141 cgacaccgta tgcctcggcg aaagggggaa agttcgacaa aggcccgttc gcccgatacc
     1201 ggttcgggga gtgcacatcg acgttggtga gcatccggag atactcatcg ctgtagttca
     1261 tccgccatat ggtggcgaaa gagacaaaga accgctgcaa cgggctcagc ccgccgagat
     1321 cgggccccgc accgtccagg gtcgagctga aggcgtcata ggcgacggcc agaccaccga
     1381 ggtcggcgat gttctcgccg agggtcagct tgccgttcac cttgagatca tcgaagaccc
     1441 cgtagtcgga gtactgctcg acgaggacct gggctctcct ctcgaactcg actctgtcct
     1501 catcgctcca ccagttgcgg cggcgacccc cctcgtcgaa gcggctgccg ttgtcgtcga
     1561 atccgtgcgt gatctcgtgc ccgatcaccg cgcctatgcc cccgtagttg acagcgtcat
     1621 cggcgtcggc gtagaagaac ggcggctgga ggatgcctgc agggaagacc acctcgttgt
     1681 gaagagggtt gtagtaggcg ttgaccacgt gggccggaag ctcccactcc cccttgtcga
     1741 cggggtcctc gagccgtgac atcttccgat cgaactcgaa tgtggctgcc cgcattcggt
     1801 tggccacgaa cgaggtacgg tcgacgacga gaagcgagta gtcacgccac tcgtcgggat
     1861 acccgatctt gtgagtgaag gtgcgcactt tcgccagcgc ggccttcttg gtggtgtcgg
     1921 tcatccactc gaggccctgg atggagcggg ccatggcttc gatgaggtgg tcgaccatct
     1981 cctcgcatcg ctccttggcc cccggcgaaa aggcctcatc gacgtagagc cgcgaaacca
     2041 gctctccgat gtcggcggtg gccgctccga ggaccctcgt ccatcgctca cgcggctcct
     2101 gttgaccacc gagcttgcgg ttgtagaagt cgaaagcctc ctcctcgaag acctgcggga
     2161 gtgccggggc gaaacgacgc accagatgcc agcggaggta gctcttgagc gtctcgatcg
     2221 aggtattggc gagcaagccg tccagca
//
LOCUS       DASUTA010000198         2954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1355381, whole genome shotgun sequence.
ACCESSION   DASUTA010000198 DASUTA010000000
VERSION     DASUTA010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355381"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VFZ80_03915"
     CDS             <1..435
                     /locus_tag="VFZ80_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6286609.1"
                     /translation="LGCDFRYMAEDARIGQPEILLGLIPGAGGTQRLQRVIGFQKTKE
                     LVYSGRQVGAEEALEIGYADKVAPGDQVLDLAMADAREWATKATLAIAAAKKALADGR
                     GMTLDAALVIEREGFHTSFATDDAVEGVNAFIEKRQADFKGK"
     gene            complement(439..873)
                     /locus_tag="VFZ80_03920"
     CDS             complement(439..873)
                     /locus_tag="VFZ80_03920"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6286610.1"
                     /translation="MARVSVSRDIPAAHDLVWKALADLNTHTSWMKDARSIEFTSDQQ
                     RGTGTRMEVLTVIGPLRTTDIMEVVGWEEGKSIDVRHEGLVTGTGTLGVEPVGDGSRV
                     TWDENLTFPWWLGGFITAWLARPVLAAVWRGNLRRLEQTLVS"
     gene            900..2033
                     /locus_tag="VFZ80_03925"
     CDS             900..2033
                     /locus_tag="VFZ80_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX6286611.1"
                     /translation="MIYLDHAATTPMRPQVWEAMAPYAGDKFGNPSGVHGVSRSAKNA
                     LEDAREQIAELIGAAPMEIVFTSGGTESDNLAIKGAVLNASERRGLVTSAIEHEAVLE
                     TADFLKRLGMPVSIIGVDDEARVDPAEVGDAVNEDTAVVSVMMVNNETGTIEDIRAMS
                     AAVKRANPDALMHSDAVQAFGCEEVDVGSLGLDLLTISAHKFGGPKGAGILYVRDGTV
                     LEPVLHGGGQELGRRSGTHDVASAVGIATAVRLAIDDRERFETEVRSVRDHMESRLVA
                     EIDDVVVNTPRTGRSSHHLNVRFPGVRNETLLMLLDQRGVAASAGSACQSGAATVSHV
                     LEAMGPTPDQARESVRFTFGWTSTKAEADGAVDIVKDLVGRLR"
     gene            2038..>2954
                     /gene="mnmA"
                     /locus_tag="VFZ80_03930"
     CDS             2038..>2954
                     /gene="mnmA"
                     /locus_tag="VFZ80_03930"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF001138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX6286612.1"
                     /translation="MKVLVAMSGGVDSSVAAALMKSAGHEVMGVTLKLWSGPRGEAPT
                     AGCCTVSDAEDARRVAAQLDIPYYVLDHTDDFKDGVVDRFVEDYASGRTPNPCIECNR
                     TVKFDRLLGRLDDFECDVLVTGHHARTRLADGRWQLARGRDLAKDQSYVLSMLTQSQL
                     ARTRFPVGELTKREVRRVAAGMGMRTAHKPESMDICFVGHRDYRGFLRDAAPDAFRPG
                     PVVDSHGTQVGVHDGIADFTVGQRRGLGVAVGEPRFVIRIEGEGATVVIGSRDQLAVD
                     GVALDQITWTDGDFRQGAVMAQYRAHGDPV"
BASE COUNT          529 a          936 c          983 g          506 t
ORIGIN      
        1 ctcggctgtg acttccgata tatggccgag gacgcaagga tcggtcagcc agagatcctg
       61 ctcggtctca ttcccggtgc cgggggcacc cagcggctcc agcgcgtcat cggtttccag
      121 aagacgaaag agctcgtcta ctcgggccgt caggtcggag ccgaggaggc actcgagatc
      181 ggctacgccg acaaagtggc accgggggac caagttctcg acctggccat ggcagacgca
      241 agggagtggg ccaccaaggc aacccttgcg atcgcggcgg ccaagaaggc gttggccgat
      301 ggtcgcggca tgactctcga cgcggcgctg gttatcgagc gggagggctt ccacacctcc
      361 ttcgccaccg atgacgcagt cgaaggtgtc aatgccttca tcgagaagcg ccaggcggac
      421 ttcaaaggga aatagcggtc agctgaccag ggtctgctcg agtcgtcgca gattccctct
      481 ccaaaccgcg gccaggacgg gacgtgccag ccaggccgtg atgaatccac cgagccacca
      541 aggaaacgtg aggttctcgt cccaggtgac ccgcgagccg tctccgaccg gttccacgcc
      601 gagggttccg gtaccggtga ccaggccttc gtgacgcacg tcgatcgact tgccttcctc
      661 ccagccgacc acctccatga tgtcggtggt tcgaagggga ccgatcactg tgaggacctc
      721 cattcgagtg cccgtgcctc gttgctggtc tgatgtgaac tcgatcgacc gggcgtcctt
      781 catccagctc gtgtgggtgt tcaagtcggc gagtgccttc cagacgaggt cgtgagcagc
      841 ggggatgtct cgggatacag atacacgtgc catgccggtt cgtggaacaa tattgcctca
      901 tgatctattt ggatcacgct gccacgactc cgatgcgacc ccaggtgtgg gaagcgatgg
      961 ccccatacgc cggagacaag tttgggaacc cttccggggt tcatggtgtc tcccgctccg
     1021 ccaagaacgc cctggaggac gccagggagc agatagccga gttgatcggc gcggccccga
     1081 tggagatcgt cttcacgtca ggtggcacgg agagcgacaa cctcgccatc aagggagcgg
     1141 tgctcaacgc gtccgagagg agaggactgg tgacctcggc gatcgagcac gaggcggtcc
     1201 tcgagacggc cgatttcctc aagcgtctcg gcatgccggt ttcgatcatc ggagtggacg
     1261 acgaggcgag ggtcgacccg gcggaggtcg gcgatgccgt caacgaagac accgccgtcg
     1321 tctcggtgat gatggtgaac aacgagaccg gcacgatcga ggacattcgg gccatgtcgg
     1381 cggcggtcaa gagagccaac ccggatgcct tgatgcacag cgatgccgtc caggcgttcg
     1441 ggtgcgagga ggtcgatgtc ggctccctgg gcctcgatct cctcaccatc tccgcccaca
     1501 agttcggagg gcccaagggc gccggcatcc tctatgtccg tgacggcacc gtcctcgagc
     1561 ccgtcctcca cggtggaggc caggagctgg gaagacgatc aggaacgcac gacgtcgcct
     1621 ccgcggtggg cattgccacc gcggtgaggc tggcgatcga cgaccgggag cgcttcgaga
     1681 cggaggtgcg ctcggtcagg gaccacatgg agagccgtct cgtcgccgag atcgacgacg
     1741 tcgtcgtcaa cacgcccagg acggggaggt cgagccatca tctgaatgtc cggtttcccg
     1801 gggtgcgcaa cgagactctg ctcatgctcc tggatcagcg aggcgtggcc gcttcggcag
     1861 gctcagcgtg ccagtccggt gccgccaccg tctctcacgt tctcgaggca atgggcccca
     1921 cgcctgacca agcgcgtgag agcgtccgtt tcaccttcgg atggacttcg accaaggcag
     1981 aagccgacgg ggccgtcgac atcgtgaagg acctggtcgg ccggttgcgg tgacgtcttg
     2041 aaggtcctcg tcgccatgtc cgggggagtc gactcctcgg ttgccgccgc tctcatgaag
     2101 agcgccggcc atgaggtcat gggggtcacc ctcaagctct ggtccggtcc gcgtggcgag
     2161 gcgccgacag cgggctgttg cacggtctcc gacgccgagg acgcccgtcg cgtggccgcc
     2221 caactcgaca ttccctacta cgtgctcgat cacaccgatg acttcaagga cggagtggtc
     2281 gaccgcttcg tcgaggacta cgcatcgggc aggaccccga atccctgcat cgagtgcaac
     2341 cggacggtca agttcgaccg tctcctcggc cgtctcgacg acttcgagtg cgacgtcctg
     2401 gtgaccggcc atcacgcccg gacccgtctc gccgatggga ggtggcagct ggctcgcgga
     2461 cgcgacctcg ccaaggatca gtcgtacgtg ctgtcgatgc tcacccagag ccagctcgcc
     2521 cggacccgat tcccagtcgg tgagctgacc aagagagagg tgcgccgggt ggcggcgggc
     2581 atgggcatgc gcacggcgca caagccggag tcaatggata tctgcttcgt cggccatcgc
     2641 gactatcgag ggttcttacg cgacgccgcg ccggacgcct tccggcccgg tcccgtcgta
     2701 gacagccatg gcacccaggt tggggtacac gacgggatcg ccgatttcac ggtgggtcag
     2761 cgacgagggc tcggtgtcgc cgtcggcgag cctcgcttcg tgatcaggat cgaaggcgaa
     2821 ggggccacgg tcgtcatcgg ctcccgtgac cagctggccg tcgacggggt cgcactcgac
     2881 cagatcacat ggaccgatgg cgacttccgc cagggtgccg tcatggccca atatcgcgct
     2941 cacggcgatc cggt
//
LOCUS       DASUTA010000199         2313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1615388, whole genome shotgun sequence.
ACCESSION   DASUTA010000199 DASUTA010000000
VERSION     DASUTA010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615388"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFZ80_03935"
     CDS             <1..372
                     /locus_tag="VFZ80_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX6286613.1"
                     /translation="TRVTFESEDRFATHHQYTDTPEGTFFCTTHIEAGREPAHAITLG
                     VRFEDARWFRGRDTPNRETSTCPDPRCCRVPAPDLSEAWSDNVIVSARSQARILGLMA
                     PDPYPELDMPEVLAVVSSHSG"
     gene            complement(369..788)
                     /locus_tag="VFZ80_03940"
     CDS             complement(369..788)
                     /locus_tag="VFZ80_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX6286614.1"
                     /translation="MERTDRDVDAFLASIEGRPGEDIRRLDAAIVERMRGAERYLYEG
                     KFWGGSDQQIVGYGVMDYENRSGENVEWFVVGLAAQKNYISMYVNAVKDGRYLLREYE
                     KELGKAKVGSASISFGSLDDVDLDGLLALVEEAGRLS"
     gene            complement(830..1426)
                     /locus_tag="VFZ80_03945"
     CDS             complement(830..1426)
                     /locus_tag="VFZ80_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012183498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX6286615.1"
                     /translation="MSTGQPGRRPGAPDTRAEILEAARSVFAERGFDRATIRAIAANA
                     GVDPAMIHHYFGTKDQLFTASIDIPAAATERVLDLLAQDPDDLGRHLAETFFGVWENE
                     EPRTALLGILRSAMGGEDRAVLAFRQFLTSVLTAQLAPKIRGEGARLRALLMASQLVG
                     VAMTRYVMRLEPIASAPIAEVIDLVAPRLQSYVDDPDG"
     gene            complement(1423..2265)
                     /locus_tag="VFZ80_03950"
     CDS             complement(1423..2265)
                     /locus_tag="VFZ80_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286616.1"
                     /translation="MIGIEASSVSKTFGEVVAVDDLSFGVAPGEVVGLLGANGAGKTT
                     TMKMILGLITPTAGEVRIGGHPVRDVDRRDVGYVPQGLGLYRDLTVAENLQFAAQAFG
                     VQPPSLGEAGLGAIADRKVGQISLGLRRRVAFLVARCHHPSVLILDEPTSGVGPLGRP
                     RLWETIHETAETGAAILVSTHHMDEAEECDRVVLMASGRGVMTGTVSEVVDRFTTVSV
                     TYEVAPGDLMSLRKAGATVLVAHPGLRIVGLGVTEVNRILGSSAGVSETPSSFEEAFV
                     ALST"
BASE COUNT          440 a          800 c          666 g          407 t
ORIGIN      
        1 accagggtca cattcgagtc cgaggatcgt ttcgcgacac accaccaata cacggatacg
       61 cctgagggga cgttcttctg caccacgcat atcgaagcgg gacgggagcc tgcccacgcc
      121 atcaccctcg gggtccgttt cgaagacgcc cgctggtttc gtggacgcga cacccccaac
      181 agggagacat cgacatgtcc tgaccctcgg tgttgccgtg tgcccgctcc agacctgagc
      241 gaagcatggt ccgacaacgt catcgtgtcg gctcgatcac aggctcggat cctggggctg
      301 atggcacccg acccctaccc ggagctcgac atgccggaag tgttggcggt cgtgagcagc
      361 cattccggct agctcagacg cccagcctcc tcgacgagag ccaacaaccc gtccaagtcg
      421 acgtcgtcga gactcccgaa gctgatcgaa gcacttccca ccttcgcctt gcccagctcc
      481 ttctcgtact cccgcaacag atagcggccg tccttgaccg cattcacata catcgagatg
      541 tagttcttct gcgccgccag ccccacgacg aaccattcca cgttctctcc ggatcggttc
      601 tcgtagtcca tcaccccgta cccgacgatt tgctggtcgc tgcctcccca gaacttgcct
      661 tcgtacaggt agcgctcagc gccgcgcatc cgttcgacga ttgccgcatc cagacggcgg
      721 atgtcctcgc cgggccgacc ttcgatcgag gccaggaatg cgtcgacgtc ccggtctgtc
      781 ctttccattg gacgagacta tgcaaccaac ccgctcatcg gacggggcct cagccgtcgg
      841 ggtcgtcgac gtaagactgc agtcgaggtg ccaccagatc gatcacttcg gcgatcggcg
      901 cgctggcaat gggctccaga cgcatcacat accgtgtcat cgccactccc accaactgcg
      961 acgccatcag gagagctctg agccgggcgc cttcgccgcg aatcttgggg gcgagctgcg
     1021 ccgtcaagac ggaggtcagg aactgccgga aagccaacac ggcccgatcc tcgcctccca
     1081 tggccgagcg caggatgccc aatagggctg ttcgaggctc ctcgttctcc cacacgccga
     1141 agaacgtctc ggcgagatgt ctccccaggt cgtcggggtc ctgggcgagc agatcgagca
     1201 ctctctcggt ggcggccgca ggtatgtcga tggacgccgt gaacaactga tctttggtgc
     1261 cgaagtagtg atggatcatg gccgggtcga cacctgcgtt ggctgcgatg gctcggatcg
     1321 ttgccctgtc gaaccctctc tcggcaaaga ccgaccgcgc cgcctcgaga atctcggcac
     1381 gggtgtctgg agctccaggt ctcctgccgg gctggccggt gctcatgtgg agagggccac
     1441 gaatgcttct tcgaacgagg atggtgtctc ggagacaccg gcggatgacc ccaggattcg
     1501 attcacttcg gtgacaccga gcccgacgat ccgcaaacca ggatgagcca caagcactgt
     1561 ggccccggcc ttccgaagac tcatcaggtc gcccggagcg acctcgtagg tgaccgacac
     1621 ggtcgtgaag cggtcgacga cctcggagac cgttcccgtc atcacccctc tccccgatgc
     1681 catcagcacg acacgatcgc actcctccgc ttcgtccatg tggtgggtgg acaccaggat
     1741 cgccgcgccc gtttccgccg tctcatggat cgtctcccag agcctcggcc ttcccaaagg
     1801 cccgacacct gacgtcggct cgtcgaggat caataccgaa ggatggtgac accgcgccac
     1861 gaggaaagcc acgcggcgac ggagcccgag cgagatctgg ccgaccttgc ggtcggcgat
     1921 ggcgcccaaa cccgcttcgc ccaacgaggg aggttgcaca ccgaaagcct gcgcggcgaa
     1981 ctggagattc tcggcgacgg tcaagtcgcg atatagaccg agcccctggg gcacatagcc
     2041 gacatcccga cggtcgacat cccgcaccgg atgtccaccg attctcacct cgccggcggt
     2101 tggggtgatc agcccgagaa tcatcttcat cgtcgtcgtc ttccccgcac cattggcgcc
     2161 gagaaggccc accacctcac ctggggcgac accgaatgag agatcgtcca ctgccaccac
     2221 ttcgccaaag gtcttggaaa cgctcgaggc ttcgatgccg atcacgacac ctccctggcc
     2281 agagccgcca cggtgatgac gtcggcgaga tca
//
LOCUS       DASUTA010000200         2463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_707428, whole genome shotgun sequence.
ACCESSION   DASUTA010000200 DASUTA010000000
VERSION     DASUTA010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707428"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VFZ80_03955"
     CDS             <1..758
                     /locus_tag="VFZ80_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6286617.1"
                     /translation="YGWPSFVMYRFVALWAGAESREVPLGDDHSLDLEAMRDAIDDDT
                     TMVIVCNPNNPTGTIKPADEIESFIDSVPEHVLVVVDEAYHEFVTDPAYRTQVPMALE
                     RPNVVVLRTFSKIYALAALRVGYAIARADLLTELRKAQAPLTVNLVAQTAARATLGQP
                     DELERRRSLNEARRHHLVGALAERGFPMAESHTNFVYFELGDDADVVIDTMTTRGVLI
                     RGMGGGWVRVTIGDDEENRRFLEALDDAFENAR"
     gene            871..>2463
                     /locus_tag="VFZ80_03960"
     CDS             871..>2463
                     /locus_tag="VFZ80_03960"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX6286618.1"
                     /translation="MKTLINAISGAVRKAPWVVIILTVILSMALGALSGNFTPAEDQN
                     ESFAPDAPELAAQATIGDLFGSSQSSMQVLIAAESGDVITLDGWEATNAIEKSVRSSA
                     LSEFLADSQDSPAIASYMAPVAFAVSQGAPAPASDAELEALYTQSYGELPSQQRGFVD
                     LLLPTDADPASAQTDLGVLVISYESSSDFDEGARRAQLAAEAVQAAELAPGITADSFS
                     QELIFANQDDFSAEILRLFISAGFIIITVLAAVYLLRPPNRVGRSLTVIGFALLLGAV
                     VLVTLPSLAAIFPDTLPDSIANWEIGPVALIGAGTLALVFTIWSVASGRLRRTVADTM
                     LTIATIFFAISWMNGLGYLIYGEQGPMTQILPILLIGLGVDYAIHLTSRYREDVATGS
                     SVDTGIRSSIRTVGIALALATVTTSIGFLTNVFNEIPALREFGALAAIGIVASFVLAL
                     TFVPAVREVVDRIGERRGTLDRESLEGNSARLLPKAIGSTSVLAKKGSVAVVLVSLGL
                     GALGGWATVTQLEARFDFLDFVP"
BASE COUNT          448 a          763 c          731 g          521 t
ORIGIN      
        1 tctatggatg gccgagtttc gtcatgtacc gattcgtcgc cctctgggca ggcgcagaga
       61 gccgggaggt ccccctgggc gacgatcact ccctcgacct ggaggcgatg agagacgcga
      121 tcgatgacga tacgacgatg gtcatcgtct gcaatccgaa caacccgacc gggaccatca
      181 aacccgccga cgagatcgaa agcttcatcg acagcgtccc ggagcacgtg ttggtggttg
      241 tggacgaggc atatcacgag ttcgtcaccg atccggccta tcgcacccag gtcccgatgg
      301 ctctggagcg ccccaacgtg gtggttctcc gaaccttctc gaagatctat gccttggcag
      361 ctctcagggt cggctacgca atcgcccggg cagacctgtt gaccgagctg cgcaaggcgc
      421 aggctcccct cactgtgaac ctggtggcac aaacggcggc cagggcaacc ctcggtcaac
      481 ccgatgagtt ggagaggcga cggagcctca acgaggctcg tcggcatcac ctggtgggcg
      541 ccctggccga aagagggttt ccgatggcgg agagccacac caatttcgtc tacttcgagc
      601 tgggtgacga tgcggacgtg gtcatcgaca cgatgaccac tcgtggggtc ctgatccgag
      661 gcatgggagg cggatgggtc cgggtcacca tcggagacga cgaggagaat cgacgtttcc
      721 tcgaggcgct ggatgatgcg tttgagaatg cccgctgatt ggatttgaat cagcggcgcc
      781 tttcgattcc accaccaggt cgatgcaaat cggatagtcg gaccggcgct actgctcgtg
      841 ccctctgcaa caacggatta ggaaccaaac atgaagacac tgatcaatgc catctctggt
      901 gctgtgcgca aggcgccttg ggtcgtgatc atcctcactg tgattctctc gatggcactt
      961 ggtgccttgt cgggaaactt cacacccgcc gaggatcaaa acgaatcgtt cgcccctgac
     1021 gcgcccgagc tggcggctca ggcgaccatt ggtgacttgt ttgggtcgag ccagagctcg
     1081 atgcaggttc tgatagccgc cgaatcgggc gacgtcatca ctctcgacgg ttgggaggcc
     1141 accaacgcca tcgagaagtc ggtccgatcg agtgctctca gcgaattcct agccgacagc
     1201 caggattcgc ctgccattgc gagctacatg gctccggtgg ctttcgcagt ctcgcagggg
     1261 gcacccgctc ccgcttccga cgccgagctg gaggcactgt acacccagtc ttacggtgaa
     1321 cttccctcac aacagagggg cttcgtcgat ctgctcctac caacggatgc ggatccagcg
     1381 agtgcgcaga ccgacctcgg cgttctggtg atcagctacg agtcgagctc tgacttcgac
     1441 gaaggcgcta gacgagctca gctcgctgcg gaggctgtcc aagccgccga gctagctccc
     1501 ggaataaccg ccgattcctt cagccaggag ttgatctttg ccaaccagga cgacttctcc
     1561 gccgaaatcc tccgtctctt catctccgct ggcttcatca tcatcaccgt tctcgctgct
     1621 gtctacctat tgcgtccccc gaaccgggtg gggcgctctc tgactgtcat cggatttgcg
     1681 cttctcctcg gtgcagtggt cttggtcact ctcccatccc tggcagcgat cttcccggac
     1741 acgctccccg actcgatagc caattgggag atcggcccgg tagcgctgat tggggcgggc
     1801 accttggccc ttgtcttcac gatctggtcg gtggcatccg ggcgtctgcg gcgaactgtc
     1861 gctgacacga tgctcacgat cgccaccata ttctttgcca tatcctggat gaatggtctc
     1921 ggctacctga tctacggcga gcagggcccg atgacccaga tcctgccgat cctgctgatc
     1981 ggtttgggag tcgactatgc catacacctg acctctcgct accgagagga cgtggccacg
     2041 ggttcctcag tcgacaccgg gatacgcagt tccattcgta cggtgggcat tgcacttgcc
     2101 cttgccacgg tcacgacgtc gattggcttc ctgaccaacg tgttcaacga gatcccggcg
     2161 cttcgtgagt ttggtgcgct cgccgcgatc ggcattgtgg cgtcattcgt gctggccctg
     2221 acattcgtgc ccgccgtccg cgaggtggtc gatcgcatcg gggagagaag gggcactctc
     2281 gatagagagt cactcgaagg caactctgct cgtctccttc cgaaggccat tgggtccact
     2341 tccgttctcg ccaagaaggg atcggtggct gtagtcctgg tctctcttgg cctcggagcg
     2401 ctcgggggat gggccactgt cacccaattg gaggcgagat tcgacttcct cgacttcgtg
     2461 ccg
//
LOCUS       DASUTA010000201         5525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1356257, whole genome shotgun sequence.
ACCESSION   DASUTA010000201 DASUTA010000000
VERSION     DASUTA010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356257"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VFZ80_03965"
     CDS             <1..656
                     /locus_tag="VFZ80_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6286619.1"
                     /translation="WNDFCSDPMVRLDRGGADFLRSVSTHFAEAGVGSVYSPALYPSA
                     TGVWRRSGFQTHAELAVMERSLNRAPPDKPIHEVSSPTQPHWESVSRLDEAAFDGFWR
                     MSRLALEEAYQTARTSALLETFVDGRLAGYAIVGNQWGVSYLHRIAVHPDHAGQGRGA
                     SLLTAAAGWGRAHGARVMVLNVREVNKRAQTLYQRLGFSHTGTRLSVLRHQAADMLN"
     gene            661..1950
                     /locus_tag="VFZ80_03970"
     CDS             661..1950
                     /locus_tag="VFZ80_03970"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX6286620.1"
                     /translation="MPAAIPDRYQLEVRLGRDGDIEEWLATDSSLDRPVLVRSLGPES
                     SQERRRQFVTAVSGAARTSHAHLARIFAVAEVEGGAYSVTEWTGGATLADRVAANQPV
                     DLPDFLPNASGLAGALAALHAAGVIHGRIDTSAISYSGAHPAKLGAFGRTATTDAGGD
                     VRALSAALETALTGSPPGGPPPSERVDGVPKTLDRILRGGQSGMSTAHELEEALRAAP
                     TPRAPRSDTGPTSRRLLLAALGLTIVAIGLVAVGILLSGGSGLVVPTPTTEGRATTTT
                     TVVEVTTTISPGEVALSQPGTLDPFGGGGENDQMVDGLIDGDITTTWQTERYQDPIEL
                     LKPGVGVTVRLQGTPSLLELERLTEGTVAVISWSEERLEQPEGWERIAEVHATPGSTA
                     VDLPPRRDGFWLIWLTELTEQDDGTYLSSIAELRFEP"
     gene            1947..2492
                     /locus_tag="VFZ80_03975"
     CDS             1947..2492
                     /locus_tag="VFZ80_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6286621.1"
                     /translation="MTTPAQEAELLARVVDGDHDAFTQIMKTHEDRVFSVCLRIMGNR
                     EQALDATQETFLTTFRKASQFKGNSALGTWIYRIAVNTCYDQLRKQKRRRTDPMPDHV
                     DPADYSAEDAVQSAGLRPEIQRALAAIPEDFRTVVILSDIEGMGLPEVAEILEIPVGT
                     VKSRVFRGRRLLAGELGNQSV"
     gene            2514..3371
                     /locus_tag="VFZ80_03980"
     CDS             2514..3371
                     /locus_tag="VFZ80_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286622.1"
                     /translation="MRDHDLELIAALAEGRLEDESEARALIASNEEARAEYEAQKLAL
                     DALSGVSPVSMTDTERASLRRDTWSDLRAGVGTSSQRSTSPWYYRWVPVAAGMFVLVG
                     LVAVLSQGGLDGIVSADQAATSETTLAASVASTAVMSEESDGAGDDADAGDGETVTEA
                     PTDTEAPTDTIADSGDVEVSDPATPAAAAYYSAEADAIRVEEESVSRSLGGVSTTELA
                     ACLEQAGLDGYEALKANPDPGEGTEADEEVPEGAVPYVAAIPFGEDLATTQVIFVSLE
                     TCEVIHVDQ"
     gene            3416..3694
                     /locus_tag="VFZ80_03985"
     CDS             3416..3694
                     /locus_tag="VFZ80_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286623.1"
                     /translation="MQEYATKFADLLEQVASRVRAMTVDRIARYIKLTGLGILAMAMG
                     LTFVIFLVWTIFGALEIPLTTAGAFAVFGVVLVVAGGILWFTRSRDES"
     gene            3684..4610
                     /gene="trxB"
                     /locus_tag="VFZ80_03990"
     CDS             3684..4610
                     /gene="trxB"
                     /locus_tag="VFZ80_03990"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX6286624.1"
                     /translation="MNHESLIIVGSGPAGLTAALYAARADLDPLVFEGVSAGGQLMIT
                     TDVENYPGFPDGIMGPELMDQFRKQAERFGARMRQVDVTDVDFSSRPFRLHVGADEYT
                     ADAVIVATGAEAKWLGVPGEKLLTGKGVSACATCDGFFFRDQRLVVVGGGDTAMEEAL
                     FLTKFASNVTIVHRRDEFRASKIMAARALEHEKIEVLWNPVVTEIHGEDTVTGVTLED
                     TVTGETTKFETDGVFVAIGHKPTTDLFVDQLDMDANGYLEIADDGSTRTSVEGVFAAG
                     DVADHVYRQAVTAAGTGCRAAIDAERWLANQE"
     gene            4677..4994
                     /gene="trxA"
                     /locus_tag="VFZ80_03995"
     CDS             4677..4994
                     /gene="trxA"
                     /locus_tag="VFZ80_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX6286625.1"
                     /translation="MAEIKTLNDGVFNDEISGEKPILVDFWAEWCGPCRVIAPLLEEL
                     AEEQDGFDIGKLNVDHNPQTAGAHDVMSIPTLILFKDGVEKKRIVGAAPKHRLLAELS
                     EFV"
     gene            5245..>5525
                     /locus_tag="VFZ80_04000"
     CDS             5245..>5525
                     /locus_tag="VFZ80_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX6286626.1"
                     /translation="MRLYRVGDEGPAIRDIQDRLAALGFGPNGDNRALFGDGTRQAVM
                     EFQKAKGLDSDGIVGPDTWRALYEAGYRLGDRIIFMRRPMIRGEDVAEL"
BASE COUNT         1047 a         1742 c         1782 g          954 t
ORIGIN      
        1 cctggaatga cttctgctcc gacccgatgg tccggctcga tcgaggcgga gcggactttc
       61 ttcgctcggt ctccacgcac ttcgccgagg cgggagtcgg ctctgtctac tcccctgctc
      121 tatacccgag cgccaccggc gtatggcggc gaagcgggtt ccagacccat gccgagttgg
      181 cagtgatgga gcgctccctg aaccgggccc ccccggataa acccatccat gaggtctcgt
      241 ccccaaccca gccccactgg gagtcggttt cgagactcga cgaggctgcc ttcgacggct
      301 tctggcggat gagtcgcctc gcactcgagg aggcgtacca gaccgcgagg acgtcggctc
      361 tgctcgagac cttcgtcgac gggcgcctcg ccggctatgc gatcgtgggc aatcaatggg
      421 gtgtctcata cctgcaccgg atagcggtgc atcccgacca tgccggtcag ggacggggtg
      481 cctctttgct caccgcagca gcgggctggg gccgagccca tggagccagg gtgatggtcc
      541 tgaacgtgcg ggaagtcaac aagagagcgc agaccctcta ccagcggctc ggcttctccc
      601 acaccgggac gagactgtcg gtccttcgtc accaagctgc cgacatgctg aactgatccg
      661 gtgcctgcag cgatacccga ccgatatcag ctcgaggttc gactcggccg ggacggcgac
      721 atcgaggaat ggctcgccac agactcatcc ctggaccgcc ccgttctcgt ccgcagcctc
      781 ggtccagaat cgtctcaaga gagacgtcgc cagttcgtga ccgccgtgag tggtgccgcc
      841 agaaccagtc atgcccacct ggccaggatc tttgccgtgg ccgaagtcga agggggcgca
      901 tattcggtga ccgagtggac gggcggcgcc acgctcgccg accgagtcgc cgccaatcaa
      961 cccgtcgatc tgcccgactt cctccccaac gcctcgggac tggccggcgc cctcgccgct
     1021 ctacatgccg caggtgtcat ccacggacgg atcgacactt cagccatctc ctattcggga
     1081 gcacatcccg ccaagctggg ggcctttggg cgtactgcca ccacagacgc cggtggtgat
     1141 gttcgcgccc tgtcggccgc attggagacc gccttgaccg ggtcacctcc ggggggcccg
     1201 ccaccttcgg agcgggtgga tggggtgccg aagaccctcg acaggatcct ccggggaggg
     1261 cagtccggca tgtcgacggc tcatgagctc gaagaggcac tgcgcgccgc cccaacccct
     1321 agagctcccc gttcggacac gggcccgacc tcgagacgat tactgctggc ggccctcggt
     1381 ctcaccatcg tcgccattgg tctggtcgcc gtggggatac tgctgtcagg tggttcgggg
     1441 ttggtggtac ccacacccac gacggaggga agggccacca ccacgacgac agtcgtcgaa
     1501 gtcaccacga ctatctcccc gggagaggtg gcgctgtccc agccgggtac gctcgatccg
     1561 tttgggggtg ggggtgagaa cgaccagatg gtagacgggc tcatcgacgg ggatatcacc
     1621 acgacctggc agaccgagcg ataccaggac ccgatcgagc tgctcaagcc gggcgtcggc
     1681 gtcaccgtgc gcctgcaagg aacaccgtcg ctactggagt tggaacggct cacggagggc
     1741 accgttgcag tgatctcatg gtccgaggag agactggagc agccggaagg ttgggagcgg
     1801 atcgcggagg tccatgccac tcccggctca acggcagttg acttgcctcc ccggagggac
     1861 ggcttctggc tgatctggct gaccgagctc accgagcaag acgacggaac ctatctgtcc
     1921 tcaatcgcag aattacgatt cgagccgtga cgaccccagc ccaggaagcc gagctactgg
     1981 cgcgcgtcgt cgacggcgac cacgacgcat tcacccagat catgaagact cacgaggatc
     2041 gcgtcttctc ggtctgtctc cggatcatgg gcaaccgtga gcaggccttg gacgccacac
     2101 aggagacatt cttgacgaca tttcgcaaag ccagccagtt caaggggaac tcagcccttg
     2161 gtacctggat ctaccggatc gccgtgaaca cctgctacga ccagcttcgc aagcagaaac
     2221 gacggaggac cgatcccatg ccggatcatg tcgaccctgc ggactactcg gcggaggacg
     2281 ccgttcagtc tgccgggcta cgacctgaga tccagcgggc cctggcagcg atacccgagg
     2341 atttcaggac cgtggtgatc ctgtccgaca tcgaggggat ggggctgccc gaagtggcag
     2401 agatcctcga gatccccgtg ggcacggtca aatcacgtgt ttttcgggga agacgcctgc
     2461 ttgccggcga gctggggaac caatcggtgt gatgagacca tcaaagagag aacatgcgtg
     2521 atcacgacct ggagctgatc gctgccctcg ccgaggggcg gctcgaggac gagtccgaag
     2581 cgcgcgctct gatcgcgtcg aacgaggagg caagggccga atacgaggcg cagaagctgg
     2641 cgctcgatgc tctgtctgga gtgtcccccg tctcgatgac cgacaccgag cgagcttcgc
     2701 ttcgtcgtga cacctggtca gacctgcgcg ctggtgtcgg cacctcgtcg caaagatcga
     2761 ccagcccctg gtactaccgc tgggtgcctg tcgcagccgg catgttcgtc ctggttggcc
     2821 tggtggcggt gctgagccag ggtgggctcg acggcatcgt gtctgccgat caggcagcca
     2881 cctccgagac gactctcgcc gcctcggtgg ccagcacggc ggtcatgtca gaggagtccg
     2941 acggcgccgg cgacgacgcc gatgccggtg acggtgagac cgtcaccgag gcacccacag
     3001 acacggaggc acccaccgac acaatcgcag actcgggaga cgtcgaggtg agcgacccgg
     3061 cgacgccggc cgcggccgcc tactactcgg ctgaggccga cgcgattcga gtcgaggagg
     3121 agtcggtctc acgctcgctc ggaggcgtgt cgaccaccga gctcgctgca tgtctagaac
     3181 aggcaggcct cgatggctat gaggcgctga aggccaaccc ggaccccggt gagggcacgg
     3241 aggccgacga ggaggtgccg gaaggggcgg tgccgtacgt cgctgcgatc ccctttggcg
     3301 aggacttggc gacgactcag gtcatcttcg tctcgctcga aacgtgcgaa gtgatccacg
     3361 tcgatcagtg agttgacggc aggatgctgt cacaccacaa ctaacctcgc ccacgatgca
     3421 ggaatatgca acgaagttcg ccgacctcct ggagcaggtg gcatcccgtg tccgggcgat
     3481 gaccgtcgat cggatcgccc gctacatcaa gctcaccggg ctgggaatcc ttgcgatggc
     3541 tatggggttg acatttgtga tcttcctggt gtggacgatc ttcggtgcgc tggagatccc
     3601 attgaccacg gcaggagcat ttgcggtctt cggtgtcgtg ctcgtcgtcg ccgggggaat
     3661 cctttggttt acgaggtcta gagatgaatc atgagagtct catcatcgtg gggtcgggcc
     3721 cggctgggtt gacggcggcc ctatatgcgg cacgggccga tctcgatccg ctcgtcttcg
     3781 aaggcgtgag cgccggcggg cagttgatga tcacaaccga tgtcgagaac tacccggggt
     3841 tcccggatgg gatcatgggc cccgagctca tggaccagtt ccgcaagcag gcggagcgtt
     3901 tcggagcacg catgcgtcag gtcgacgtca ccgacgtgga cttctcctcc cgccctttcc
     3961 ggctccacgt cggggcagac gagtacaccg ccgacgccgt gatcgtggcc acgggtgcag
     4021 aggcaaaatg gctgggagtc ccgggtgaga agctcctgac cggcaagggt gtgtcggcct
     4081 gtgccacatg cgatggcttc ttcttccgtg accagcgtct tgtggtggtc gggggcggcg
     4141 acaccgccat ggaagaggct ctattcctca cgaagttcgc ctccaacgtg accattgtcc
     4201 atcgcaggga cgagttcagg gcttccaaga tcatggcggc ccgtgccctc gagcacgaga
     4261 agatagaggt gctctggaac cccgtcgtca cggagataca cggtgaggac actgtgacgg
     4321 gtgtcactct cgaggacaca gtcaccggtg agaccacgaa gttcgagaca gatggcgtct
     4381 tcgtcgccat cgggcacaag ccgaccaccg acctattcgt cgatcaactc gacatggacg
     4441 ccaacgggta tctcgagatc gccgacgacg gcagcactcg gacctcggtc gagggggtgt
     4501 tcgccgccgg cgacgttgcc gatcacgtct accgccaggc cgtcaccgcg gcgggcacgg
     4561 gctgccgcgc cgctatcgac gccgagcgtt ggctggccaa ccaggaatga gtgagagatc
     4621 aggcgcgttc gggtagatag acgacacaga cctcaaagga aagaacagga gaagcagtgg
     4681 cagagatcaa gaccctcaat gatggagtct tcaacgacga gatcagcggt gagaagccca
     4741 tcctcgtcga cttctgggcc gagtggtgcg gtccctgccg tgtgatcgct cccttgctcg
     4801 aggagctcgc cgaagagcag gacggcttcg acatcggcaa gctcaatgtc gaccacaacc
     4861 cgcagacagc aggggctcac gatgtaatga gcatcccgac gctgatcctc ttcaaggacg
     4921 gcgtggagaa gaagcgcatc gtcggagccg ctcccaagca tcgtctcttg gccgagctct
     4981 ccgagttcgt ctaacggttc caggccccag atatagaaca gaaggactcg accgaccggc
     5041 gtctgttcgg gtggcgctca cccgttttcc ccaccgaccg agctgatgat cgtcatctgg
     5101 ctcgagaacg ggtcgggcac gaggagacgc acacccgaag tcggtcgaca tcgaatcagc
     5161 tgcgcggccg gtgggtaccg cccgcgttca ggcaggcccc taccccatac ttcatactcg
     5221 atccctcata agatcaccgc actaatgcgt ctctatcggg ttggtgacga agggcccgcc
     5281 attcgcgaca tccaggaccg gctggctgcg ctcggattcg gcccgaatgg cgacaaccgg
     5341 gcgctgttcg gcgacgggac gagacaggcc gtaatggagt tccaaaaggc caaagggctg
     5401 gactcagacg gcatcgtcgg ccccgacact tggcgggctc tatacgaagc cggttaccgc
     5461 ctcggcgacc gaatcatctt catgagacgt ccgatgatcc gtggggaaga cgtggccgag
     5521 ctcca
//
LOCUS       DASUTA010000202         6810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_60301, whole genome shotgun sequence.
ACCESSION   DASUTA010000202 DASUTA010000000
VERSION     DASUTA010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60301"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1033
                     /gene="argC"
                     /locus_tag="VFZ80_04005"
     CDS             110..1033
                     /gene="argC"
                     /locus_tag="VFZ80_04005"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX6286627.1"
                     /translation="MPETYRVGVVGARGHTGAELMQILDDHPYVEVAFAGSRELADEP
                     VPGIEGLTFENIGPDEVAQRELDAVFLALPDGIGGPYVDEIGDDTVIVDISADHRFDD
                     DWVYGLPELFRGKVQGARRIANPGCYATALQLAIAPFAEDIDGVPTVFGVSGYSGAGT
                     TPSPRNDPENLEDNLIPYRLTGHNHEKEGIRYLGRPVRFMPHVHPAFRGLLVTAHMPL
                     AESTTVEVAHQRLTNAYDGEALVDVQEEIPTLKDGTMRKGVLIGGTTVSEDGLGLTVI
                     AVEDNLLKGAAVQAVQNLNLALGVPEISGIL"
     gene            1030..1659
                     /locus_tag="VFZ80_04010"
     CDS             1030..1659
                     /locus_tag="VFZ80_04010"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX6286628.1"
                     /translation="MSGHDSPGITVVGTGSAECVPDLMTVDIGVSVRSETVSDASSQT
                     RDHVSSLLDALTGAGLQREDIATRDFSVYPEYDHRDGQQRLLGYRVTNELSVTIRDVP
                     AAGSILDAAVSAVGDPVTINRVQLSVDDETAARDLAREAAWGDAIARAEHLARLADRS
                     LGPALSIVESTGHAPPPRPTMRAAMAEATPIEAGSASTTVTLEVRFGLD"
     gene            complement(1656..4910)
                     /locus_tag="VFZ80_04015"
     CDS             complement(1656..4910)
                     /locus_tag="VFZ80_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009884824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX6286629.1"
                     /translation="MSTYLRYPHIHDDLITFVAADDVWLAPVEGGRAWRLTDDREPVR
                     TPRFSPDGTAVAFISLRDGHPEAYVIPVDGDGGARRLTWWGAKNTLVLGWTRDGRVLV
                     ASHAGEFNLRHTVVKAVGLDGSVERLAYGPAWGVAESDEGAVALSTPGSRPPSHWKRY
                     RGGAAPRLWIDPTGDASWVQLLAGEPASLVDPMWVDGRLVFVSDRAAKLPGTAEEAGE
                     QANLWAFAGGTPDESSQAEQLTDQEAELGYVRDASTDGRRVVWHSRGRIFLLDDLDSE
                     PLEISLTTPGAAPEAFQAKSTKNLSRPRPDHGGDASLIAWRGNGYWLTHREGPARALA
                     VGSGVRVREPVLLGRTGYGVFATDVEGDDALEIQHLSGEEAPRRIAGGELGRVLSMAS
                     DSDGDRLAVISHDGSISIVDVESGSVSQIGISPEGEALSPSFSPDGRYLLWSQPIESS
                     LGLHQLMIFDTRSGEDVVPLTSGTFHDHDPAFTFDGKHVVFLSERTFDPEYDPHEFAL
                     SFAGSTRPWLIPISASEPAPFGPSAEGWRISSPENDDSKEGEEGEEAEEEASPELDAE
                     GSEERAVPLPVPSGEYRRLSTCKAGILWIEEHREAGVLGTRRAGVKDEPAPDAIQLWS
                     LQKRAVETVADKVSDYAVSGDGRNLVVRYKESVTVTPADRKPKDEKGPSVVKVDLSRL
                     RFEVEPRVEWAQMFDETARLMRDHFWREDMDGVDWDSVVDRWRPVVDSLCSHDDLVDL
                     LWETVAELNTSHAYVIPTEPPGDQKRRLGLLGADLSPAGDGWRIDRILPGESSDPAAR
                     SPLRGAGVDAHEGDLIVAVDGNPVDPNVGPARHLIGAAGKPIELTLRRNGDDRRVVVV
                     PIDDEEVLRYQDWVRSRRDYVDEHSEGRLGYVHVPDMTSTGWAQLHRDWRHATEREGV
                     IVDVRYNRGGHTSQLVLARLSKKLVGWTIARHYQSAGSYPVSAPRGPVVLVANEYSGS
                     DGDIINAASQAVGLDGVVGRRTWGGVVGIDGRYSLVDGTSVTQPRYAFWLEGKDWGVE
                     NHGVDPDIEVIHSPADFFSDADPQLDRAMEEALSILETRPALTEPPLPEPKVRR"
     gene            4948..6258
                     /locus_tag="VFZ80_04020"
     CDS             4948..6258
                     /locus_tag="VFZ80_04020"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEX6286630.1"
                     /translation="MTYTAVELIETKRDGGALSSDAIEWLISAYTEGSVPDYQMSALA
                     MAILFNGMTPDELATWTRAMLESGEVMDFSDIAASKVDKHSTGGVGDKISIPLAPLVA
                     ACGVAVPMMSGRGLGHTGGTLDKLESIPGFVTGLDADRFREILTEHGVVLAGQSGSLA
                     PADQKLYALRDVSGTVPSIPLIASSIMSKKLAEGLDGLLLDVKTGAGALMKEIEQSRE
                     LAHTMVGIGESYGVNTIAFITSMEQPLGSEVGNANEIRESIDVLSGGGPDDVIELTMA
                     FAEAMLNLGGVEGGRDRLSQAIESGAALDKFIEVTIAHGGDPRVIEDPSLLAVAPKEA
                     VIAAPYGGYVTRCDALTIGSAGVRLGAGRAEKDDVIDPGVGISVRAKLGDLVNAGDPL
                     AVVRYTEESRWADQRSHLASAWAISEERRPPPPLITQRIDATAF"
     gene            6326..>6810
                     /locus_tag="VFZ80_04025"
     CDS             6326..>6810
                     /locus_tag="VFZ80_04025"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HEX6286631.1"
                     /translation="MKVVVCVKQIPDPATPYSLEEGTNWLVRPEEQVLDDTDRYGVEM
                     GLQLAQANEGTVTLVSMGPTGNAQGIRQALAMGADKAVIVEDDSLRGSDALTTARVLA
                     KAIDGEGFDLVIAGTESTDGYSGVIPQMLAELLDVPALTYATSVNTDGSGVTIHRQTV
                     EG"
BASE COUNT         1232 a         2233 c         2186 g         1159 t
ORIGIN      
        1 aacggctgga ccatcttctg gaagggcgtc ttcgacttcg agctcatcgg gcgtgccgtg
       61 aagcgccttt cagagatgcc cccctccttc gtcgcagagc cggccgacca tgcctgagac
      121 ctacagggtg ggggtggtcg gtgcccgggg ccacaccgga gccgagctga tgcagatcct
      181 cgacgatcat ccctacgtcg aggtcgcctt cgccggctca cgtgagctcg ccgacgagcc
      241 cgtgcccggc atcgagggcc tgaccttcga gaacataggg cccgacgagg tggcacagcg
      301 agagcttgat gccgtcttcc ttgccctgcc cgatggcatc ggaggtcctt acgtcgacga
      361 gatcggagat gacacggtga tcgtcgacat ctccgcagac catcgcttcg acgacgactg
      421 ggtgtatggg ctcccggagt tgttccgggg gaaggtccaa ggggcacgtc gcatcgccaa
      481 cccgggctgc tatgccacag cgctccagtt ggccatcgca cccttcgcag aggacatcga
      541 cggcgttccc accgtgttcg gcgtatcggg atactccggc gcaggcacca ccccttcacc
      601 gcggaacgat ccggagaacc tggaggacaa cctgattccg taccggctca ccggccacaa
      661 tcacgaaaag gaaggcatcc gctacctcgg tcgaccggtc cgtttcatgc ctcatgttca
      721 ccccgcgttt cgcgggctgc tggtcacggc gcatatgccc ctggcggagt cgaccaccgt
      781 cgaggtcgct caccaacgac tgacaaacgc atatgacggc gaagccctcg tcgacgtcca
      841 ggaagagatc ccgactctga aggacggcac catgcgaaag ggcgtgctca tcggagggac
      901 caccgtgagc gaggatgggc tcggattgac cgtgatagcg gtggaggaca acctgctgaa
      961 aggagcggcg gtgcaagcag tccagaacct caacctcgcc ctgggcgtac ccgagatctc
     1021 gggaatcctg tgagcggcca cgacagcccg ggcatcacgg tcgtcggcac aggatcggct
     1081 gagtgcgttc cagacctgat gaccgtcgac atcggtgttt cggtgagatc cgagacggtc
     1141 tccgatgcct catcacagac acgcgaccac gtctcttcac tgcttgacgc cctcaccgga
     1201 gccggcctcc agcgcgagga catcgccacc cgggacttct ccgtgtatcc cgagtacgac
     1261 catcgcgacg ggcaacaacg tctgctcggc tatcgcgtca ccaatgagct gtcggtgacc
     1321 atccgggacg tgcctgcggc gggatcgatc ctcgatgcgg ccgtttctgc ggtgggtgac
     1381 cccgtgacca tcaaccgggt tcagctgtcg gtcgatgacg agaccgccgc tcgagacctc
     1441 gcccgcgagg ctgcgtgggg agatgccatc gcccgcgccg agcatctggc caggctggcg
     1501 gatcgctcac ttggcccggc cttgagcatc gtcgaatcga cggggcacgc tccccctccg
     1561 agaccgacga tgcgagccgc catggccgag gcgacgccga tcgaagcggg cagtgcatcg
     1621 acaacggtca cgctcgaggt gcggttcggg ctggactagc ggcgcacttt gggctcgggc
     1681 agcggtggct cggtgagcgc cggccgggtc tcgaggatcg acagcgcctc ttccatcgcc
     1741 cggtcgagct gtgggtccgc atccgaaaag aagtccgcag gcgagtggat cacttcgatg
     1801 tcggggtcga caccgtggtt ctcaacgccc cagtccttgc cctcgagcca gaacgcgtag
     1861 cggggttggg tgacggatgt cccgtcgacg aggctgtatc ggccatcgat gccgaccacg
     1921 ccaccccagg tcctccgtcc cacgacaccg tcgagaccca ccgcctgact cgccgcattg
     1981 atgatgtcgc catccgaccc cgagtactcg ttggcgacga gaaccaccgg cccacgcggc
     2041 gccgacaccg gataggaccc ggcggactga tagtgacggg cgatcgtcca gcccaccagc
     2101 tttttcgaca gacgagccag aacgagctgg ctggtgtgcc caccccggtt gtaacggacg
     2161 tcgacgatga cgccttctcg ctcggtggca tgtctccaat cgcgatggag ctgggcccag
     2221 ccggtgctcg tcatgtccgg gacgtggacg tagccgagac gtccctccga gtgctcatcg
     2281 acatagtccc ggcgggatcg aacccaatcc tggtagcgca acacttcctc gtcgtcgatg
     2341 ggcaccacca ccacacggcg atcgtcaccg ttgcgacgca gtgtcagctc gatgggcttg
     2401 cctgcagcac cgatcagatg gcgtgcaggc ccgacattcg ggtcgaccgg gttcccgtcg
     2461 acggcgacta tcaggtcgcc ttcgtgggcg tcgacacccg cgccgcgcag cggcgacctc
     2521 gccgcaggat cgctggactc tccggggagg attcggtcga tccgccaacc atcgcccgcc
     2581 ggagacaggt cggcaccgag taggccaagc cgccgcttct gatccccggg aggctcggtg
     2641 gggatcacgt aggcatgcga tgtgttgagc tcggcgacgg tctcccaaag aaggtccacg
     2701 aggtcgtcgt ggctgcacag cgagtcgacg acgggccgcc accgatcgac aacagaatcc
     2761 cagtcgacgc cgtccatgtc ctcccgccag aaatgatcac gcatcagcct ggccgtctcg
     2821 tcgaacatct gggcccactc gacccggggc tccacctcga accgcagccg ggagaggtcg
     2881 accttgacca ccgaggggcc tttttcgtcc ttgggtttcc ggtcggcggg ggtgaccgtc
     2941 accgactcct tgtagcggac gaccagattt cgaccgtcac ccgacaccgc ataatcgctc
     3001 accttgtcgg cgacggtctc gacagcgcgc ttctggagcg accagagctg aatcgcgtcc
     3061 ggtgccggtt cgtctttgac cccggcccgc cgtgtgccca gcaccccggc ctcccggtgc
     3121 tcctcgatcc agaggatccc ggccttacac gtcgagagac gccgatactc accggaagga
     3181 accggcagag gcacggctcg ctcctcggag ccttcggcgt cgagctcggg cgaggcctcc
     3241 tcctcagctt cttcgccctc ttcgccttcc ttcgaatcgt cgttctccgg tgagctgatc
     3301 ctccagccct cggcactcgg cccgaacggc gcaggttcac tcgccgagat ggggatcagc
     3361 caaggtcggg tgcttccggc gaaggagaga gcgaactcgt gggggtcgta ctcggggtcg
     3421 aaggttcgct cggagaggaa caccacatgc ttcccatcga aagtgaatgc cggatcgtga
     3481 tcgtgaaagg tgcccgaggt gagcgggacg acatcttctc ccgacctggt gtcgaagatc
     3541 atgagctgat gaaggccgag cgatgactcg atgggctgtg accagaggag atatcggccg
     3601 tccggggaga agctgggaga tagcgcctcg ccttccggcg agatcccgat ctggctgacc
     3661 gacccgctct cgacatcgac gatcgagata ctgccgtcgt gggagatgac cgcgagacgg
     3721 tcaccgtcgg aatcgctggc catggagaga acacgtccca gctcgccgcc ggcaatcctt
     3781 cgcggggcct cctcaccgga caggtgctgg atctccaggg cgtcgtcccc ctcgacgtcc
     3841 gtggcgaaca cgccgtaacc ggtgcgtccg agcagtacgg gctcgcgcac tctcactccg
     3901 gaacccactg ctaaggcacg tgccgggccc tcgcgatggg tgagccaata gccgttgccg
     3961 cgccaggcga tgaggctggc gtcgccaccg tggtcggggc ggggtcggct caggttcttc
     4021 gtcgatttcg cctggaatgc ttccggcgcg gcacctggcg tcgtcaggga gatctccagt
     4081 ggctcgctgt cgagatcgtc gagcaggaag atcctccctc gactatgcca cacgacacgg
     4141 cgcccgtcgg tggacgcgtc ccgcacgtag ccgagttcag cctcctgatc tgtcagctgc
     4201 tcggcttgcg acgactcgtc cggcgttccc cctgcaaagg cccataggtt tgcctgttct
     4261 ccggcctctt cggccgttcc cggcagcttc gcggcccgat cggacacgaa cacgagacgt
     4321 ccatccaccc acatcgggtc gacgagggaa gcgggctcgc cagcaaggag ctgaacccag
     4381 gatgcgtcgc cggtcggatc gatccagagc cgaggcgcgg ccccaccccg atatcgcttc
     4441 caatgcgacg gaggacgtga ccccggtgtg ctcagtgcga ccgctccttc gtcggactcg
     4501 gccacacccc aggccgggcc gtaggccaat ctctcgacgg aaccatcgag acccactgct
     4561 ttgaccacgg tgtggcgcag attgaactca ccggcgtggg aggcgaccag aacccggcca
     4621 tctctggtcc aaccgaggac gagggtgttc ttcgctcccc accaggtgag acggcgcgct
     4681 cccccatcgc catccaccgg gatcacatag gcctcgggat ggccgtcacg tagcgagatg
     4741 aaggcgacag ctgtgccgtc gggcgagaac cttggagttc gaaccggctc tcgatcgtcg
     4801 gtgagtctcc aggcgcgccc tccttcgacc ggcgccagcc agacatcgtc ggcagccacg
     4861 aaggtgatca gatcgtcatg aatgtgcgga tagcgcagat aagtgctcac cgggcgaggt
     4921 taactctgag gctgtagctt cccccccatg acatacacgg cggtggagct gatcgagacc
     4981 aagcgcgatg gaggcgcgtt gagctccgac gcgatcgagt ggctcatctc cgcctacacc
     5041 gaggggagcg tccctgacta tcagatgtcg gcactggcca tggcgatcct cttcaacggg
     5101 atgacccccg acgagctcgc cacctggacg agagccatgc tcgagtccgg ggaggtgatg
     5161 gacttctccg acatcgcagc cagcaaggtg gacaaacact ccaccggggg cgtcggcgac
     5221 aagatctcga taccgctggc gcccctggtg gcagcgtgtg gggtcgccgt cccgatgatg
     5281 tcgggccgcg ggttgggcca caccgggggc acgctcgaca agctggagtc gatcccgggc
     5341 ttcgttaccg ggctcgatgc cgatcggttc agagagatcc tgaccgaaca cggcgtggtt
     5401 ctcgccggcc agtcgggcag tctggccccc gcggatcaga agctctatgc gctccgcgat
     5461 gtctccggca ccgttccgtc gatcccgctg atagccagct cgatcatgtc caagaaactc
     5521 gccgagggcc tcgatggcct cctgctcgac gtgaagacgg gtgccggggc cctcatgaag
     5581 gagatcgagc agtcgcggga gctggcgcac accatggtcg gtatcggaga gtcttacggg
     5641 gtcaacacaa tcgcattcat cacttcgatg gaacagcccc tgggcagcga ggtggggaac
     5701 gccaacgaga tcagagagtc catcgacgtc ctgagtggtg ggggccctga tgacgtcatc
     5761 gagctcacca tggcatttgc cgaggccatg ctgaatctcg gcggggtcga agggggtcgt
     5821 gaccgcctga gccaggcgat cgagtcgggc gccgccctcg acaagttcat cgaggtcacg
     5881 attgcccacg ggggagatcc gagagtgatc gaagacccgt ccttgctggc cgtggccccg
     5941 aaagaggcgg tgatcgccgc cccatacggc gggtatgtga cccgctgcga cgccctgacg
     6001 atcggctccg ctggtgttcg cctcggggca gggcgtgctg agaaggacga cgtcatcgat
     6061 cccggtgtcg gcatcagcgt gagagccaag ctcggagacc tggtgaatgc cggtgatccc
     6121 ctggccgtcg tgcgctacac cgaagagtca cgctgggccg atcaacgctc acatctcgcc
     6181 tctgcctggg cgatctccga ggagagacga ccgccgccgc cgctgatcac acaacgcatc
     6241 gacgccaccg cattttgagc gaatactcaa ttgtgaaggc agtagttacc atcgccacgc
     6301 aatcgattca caggagaatg cggatatgaa ggtcgtcgtc tgcgtcaagc agatcccgga
     6361 ccccgccacc ccttattcgt tggaggaggg caccaattgg ctggtgcggc ctgaagagca
     6421 ggtcctcgac gacacagacc gctacggggt ggagatgggt cttcaactgg ctcaggccaa
     6481 cgaggggaca gtcacgttgg tgagcatggg acccactgga aacgctcagg gaatccgcca
     6541 ggctctggcg atgggtgcgg acaaagcggt gatcgtcgaa gacgacagcc tgcgcggatc
     6601 ggatgccttg accacggcgc gagtgctggc gaaggccatc gacggcgagg ggttcgacct
     6661 ggtcatcgca ggcaccgagt cgaccgacgg ctactccggg gtcatccccc agatgctggc
     6721 agagctgttg gatgtccccg cgctgacata tgcgacatcg gtgaataccg acgggtcggg
     6781 tgtgaccatt caccgccaga ccgtcgaggg
//
LOCUS       DASUTA010000203         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1874385, whole genome shotgun sequence.
ACCESSION   DASUTA010000203 DASUTA010000000
VERSION     DASUTA010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1874385"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1089
                     /locus_tag="VFZ80_04030"
     CDS             199..1089
                     /locus_tag="VFZ80_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6286632.1"
                     /translation="MLEVENLSKTYGEVVALDQCSFVVHPGKLTGFVGPNGAGKTTTM
                     RAVFGLVVPDEGAIRWQGSPITRTTRKRFGYMPEERGLYPKMQVREQVIHFGFITGMN
                     RTEAAHAADRILERLGLSERATASVEELSHGNQQRAQLAVALVHDPELLILDEPFAGL
                     DPIAVGNLGGMLEDLAAEGRTVLFSSHQLDLVEDLCEDVVTINNGQIVLAGKLSELRG
                     RARRWHVRVEVANAESDWFQALPGVEIIEQTNGNVELVADRSVDVGSIVSQAQEAGEI
                     RQFDYDPPTLSQLFREAVGS"
     gene            1086..2279
                     /locus_tag="VFZ80_04035"
     CDS             1086..2279
                     /locus_tag="VFZ80_04035"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6286633.1"
                     /translation="MSQVRLVLGLARREALERLRSRAFQISTVIVLLAAVAAVVLPSV
                     FLDDGPTTYRVGVAAGVSPGVEQTLAASGSTDVVVEVEAYPDRSAGEAALNDGLDVLV
                     ASDSEMVWAAEPEVRLESLVRNAMTQGQLTRRADELGLDPDELSELLAPAAIESTSLT
                     NDGTDQRRGPQALIATVGMILLFVSITYYGSHILMSVIEEKQNRVIEVLLAHVEPRHL
                     LAAKVLGHGALGLAQMIALALIAGIGLVVFDPIDVPETAFGAIASAVMWFLLGYAFYS
                     VLYGALGSLASRTEDAQSAIGPLTIVLMAAYFLTFSIIGNPSSPIAVVGSFLPPTAPL
                     MMPLRAALTDVPIWQVATAVVIQVVSIYGLVRVGGRLYRGAVLKVGKKLSIREAWNVT
                     AGDPA"
     gene            2433..>2983
                     /locus_tag="VFZ80_04040"
     CDS             2433..>2983
                     /locus_tag="VFZ80_04040"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX6286634.1"
                     /translation="MTVRSSEVAETRSTARDSIRGHPLVAFVALTYLLTWSLWLISFL
                     LGGQESIAGSVAFVAGGLGPAAAAAIVLKVSGRSLKAWGRSLVKWRVPVPFWLYALGL
                     PALILFAANLTLVMLGEPVEWSLIGERVGPYFMTFLATLFLLGAMEEPGWRGFALPRL
                     QQRFSPMKATMILGVVWGLWHLPL"
BASE COUNT          509 a          899 c          944 g          631 t
ORIGIN      
        1 ggtcctgcgg aggtcaggtt ctccaacctc acacccccct ccctggccac gtagccgccc
       61 ccggccttcg gtcacactcc cctgggactt catgctctgg agacgcgggc gcatctttcg
      121 tagattcgac cccaaagaag gacaggaacg accgatgtcc atcggcctga tcttcattca
      181 cgcccgacag gaggtcagat gcttgaggtc gagaacctct ccaagaccta tggggaggtt
      241 gtggccctgg atcaatgcag ctttgtggtt catcccggca agttgaccgg gttcgtgggc
      301 ccgaatggtg cagggaagac caccaccatg agagccgtct tcgggctggt cgtccccgac
      361 gagggtgcga ttcgctggca aggctctccc ataacccgta ctacccgaaa gaggttcggg
      421 tacatgcccg aagagcgcgg tctgtacccg aagatgcagg tgcgggaaca ggtgatccac
      481 ttcggcttca tcaccggcat gaaccgaacc gaggcggctc atgctgccga ccggatcctg
      541 gagcgcctcg ggctgtccga gcgagccact gcctccgtcg aggaattgtc ccatggcaac
      601 caacagcgcg cccagctcgc tgtggccctg gtgcatgatc cggagctgtt gatactcgac
      661 gagccgtttg ccgggctcga tccgatagcg gtcggaaatc tgggaggcat gttggaggac
      721 ctggcggcag aggggagaac cgtcctcttt tccagtcatc aactggatct ggtggaggat
      781 ctgtgtgaag acgtggtgac catcaacaac gggcagatag tgctggccgg aaagctgagc
      841 gagctacgtg gtcgcgcccg gcgctggcat gttcgtgtcg aggtggcaaa cgctgagtcc
      901 gactggttcc aggcgctccc cggcgtcgag atcatcgaac agacaaacgg caacgtcgaa
      961 ctggttgcag atcgcagcgt cgacgtcggg tcgatcgtca gtcaagctca ggaggcgggc
     1021 gagatcagac agttcgacta cgacccgcca accctctcac aactattcag agaggcggtg
     1081 ggctcatgag tcaagtgcgg ctcgtgttgg ggctggccag acgcgaggct ctggagcggc
     1141 tccgcagccg agcctttcag atctcgaccg tgatcgttct gctggctgcg gtcgcggctg
     1201 tggttctccc ctccgtgttc ctggatgatg gacctaccac gtacagggtc ggagtggcgg
     1261 ccggcgtttc gcccggtgtg gaacagaccc tggccgcatc cggatcgacg gacgtcgttg
     1321 tcgaggtcga ggcctacccc gaccgttccg ctggtgaggc cgccttgaat gacggactgg
     1381 acgttctggt ggcttccgac agcgagatgg tgtgggcagc cgaacccgag gtccgactcg
     1441 agtcgcttgt ccggaatgcc atgacccagg gacagctgac acggcgtgcc gacgagctcg
     1501 ggctcgaccc agacgagctg tccgagctgc tggctcctgc ggccatcgag tccaccagtc
     1561 tgacaaacga tggcaccgac cagcggagag gacctcaggc gctgatcgcc accgtcggca
     1621 tgatcctgct ctttgtttcg atcacctact acgggtctca cattctgatg agcgtcatcg
     1681 aggagaaaca gaaccgggtc atcgaggtgc tcctggccca tgtcgaacct cgtcatctcc
     1741 tggctgcgaa agtgctcggt cacggtgcgc tcggactggc ccagatgatc gcgttggcgc
     1801 tcatcgccgg tatcggtctg gtcgtcttcg atcccatcga cgtgcccgaa accgccttcg
     1861 gggccatagc cagcgcggtg atgtggttcc tactcgggta tgccttctac agcgttctct
     1921 acggggcgct tgggtcactc gcctcccgca ccgaggacgc ccaatcggcg atcgggccac
     1981 tgaccatcgt tctcatggcg gcctacttcc tgaccttctc gatcatcggc aacccatcga
     2041 gccccatagc tgtcgtcggg tcattcctgc ctcccacggc acccctgatg atgccgctgc
     2101 gcgccgccct cactgatgtt cccatctggc aggtcgccac ggccgtggtg attcaggtcg
     2161 tctccatata cggtctggtt cgggttggag ggcggctcta tcggggcgct gtgctgaagg
     2221 tcgggaagaa gctgtcgatc cgcgaggcat ggaatgtcac cgctggcgac ccggcatgac
     2281 gggctcgtcg catcctcgca gcttcggttg atgactctgg aacagggtcg ccggtgcaag
     2341 gacgatcgtc gtggtcaatc ggggtcaatg accctgtcgg gactccaacc tgcatgtaca
     2401 cggtggagac gtctgtcaaa ggaggcgttt gcatgactgt ccgttccagt gaggtagccg
     2461 aaaccagatc cacagctcgg gattcgattc gaggtcatcc attggtcgcc ttcgtggccc
     2521 tcacctatct attgacctgg tcgctctggc tgatttcttt tctcttggga gggcaggagt
     2581 cgatcgctgg gagcgttgct ttcgttgcgg ggggactcgg gcctgctgct gccgctgcga
     2641 tcgttctcaa ggtttcgggg aggtcgctga aggcttgggg tcggtcgctc gtgaagtggc
     2701 gtgttcccgt tccattctgg ttgtacgcgc tgggacttcc ggccctgatc ctctttgcag
     2761 ccaacctgac gctcgtcatg ttgggtgagc ctgtcgaatg gtcgctcatc ggcgaaaggg
     2821 tcggacccta cttcatgaca ttcctggcaa cactgtttct tctcggcgcc atggaggagc
     2881 ccggttggcg tggcttcgcc ttgccccggc tacagcaacg attcagtccg atgaaggcaa
     2941 ccatgatcct gggtgtggtg tgggggctat ggcatctgcc tct
//
LOCUS       DASUTA010000204         3411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1744456, whole genome shotgun sequence.
ACCESSION   DASUTA010000204 DASUTA010000000
VERSION     DASUTA010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744456"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VFZ80_04045"
     CDS             complement(<1..286)
                     /locus_tag="VFZ80_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286635.1"
                     /translation="MTVGAAFRERIDSWDTLSRWERSELGKDLRRLGLSYGEIMDLIP
                     VKKSTLATWYRDVKLTDEQVEALKIRRSPIPGIPRNTQRKRHREIEMITAQ"
     gene            348..421
                     /locus_tag="VFZ80_04050"
     tRNA            348..421
                     /locus_tag="VFZ80_04050"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:382..384,aa:Pro,seq:tgg)
     gene            446..516
                     /locus_tag="VFZ80_04055"
     tRNA            446..516
                     /locus_tag="VFZ80_04055"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:478..480,aa:Gly,seq:tcc)
     gene            554..627
                     /locus_tag="VFZ80_04060"
     tRNA            554..627
                     /locus_tag="VFZ80_04060"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:588..590,aa:Arg,seq:tct)
     gene            653..727
                     /locus_tag="VFZ80_04065"
     tRNA            653..727
                     /locus_tag="VFZ80_04065"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:His,seq:gtg)
     gene            807..2210
                     /gene="tig"
                     /locus_tag="VFZ80_04070"
     CDS             807..2210
                     /gene="tig"
                     /locus_tag="VFZ80_04070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX6286636.1"
                     /translation="MPTTVTETGPFERLVVFQLTDDQIAAGKAATARKLSHDLKLKGF
                     RPGKAPLPVVEAAVGPARLRSEVIDDLVPPVLTDILNDEELRPAVTPQLEAMNDVEGG
                     VEVEVRVTLWPVVDLPNYKNREVEVTSPEVTPEELESQLERMLEQFATVEEANRPART
                     GDFVSVDVKATSEGESVEGASAADLLYEVGSGLLVEGVDERLEGAEPGTVLTFDAPLP
                     GGFGERAGEEVSFEIVVNEVKERILPALDDEWVDENTEFETVEELRAELEERLGEAKV
                     QAVSRQFAERALSTLVDQVEIELPDALVRSEMDDQLHRFVHRLEDNELTLDDYFQASG
                     IDQEQFLDDLRQQAERSLRNQLVLEAVAKDAGIEVTPDDVSSVIQAAAAQSGDPVAYL
                     NAFRQGGRELALAGDILRNRALDAILSAANPVDEEGNPVDLKLNMTEVEAEIVEGELV
                     GDYPAGEIVDAVAEEEE"
     gene            2207..2836
                     /locus_tag="VFZ80_04075"
     CDS             2207..2836
                     /locus_tag="VFZ80_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX6286637.1"
                     /translation="MTAENQFQNYVIPTVFEQTSRGERYFDIYSRLLKDRIIFLGTPI
                     DDTIANLIMAQLLHLESEDPDKDIFLYINSPGGSITSLFAIYDTMQYIKPDVNTVCMG
                     MAASAAAVILAGGAEGKRYALPHARVMLHQPHGGAQGQASDIEIQAKLIVQMREQLNE
                     ILAHHTGQEYDKIALDTERDYWMLADAAKEYGVIDGILTRRELAAVASK"
     gene            2857..>3411
                     /locus_tag="VFZ80_04080"
     CDS             2857..>3411
                     /locus_tag="VFZ80_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007264060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HEX6286638.1"
                     /translation="MARLGDGGELLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEEFSVTEEITFTELPKPVEINSYLDDYVVGQEDAKKKLSVAVYNHYKRIRGGEIQ
                     RDVELQKSNILLIGPTGSGKTLLAQTLARQLNVPFAIADATSLTEAGYVGEDVENIIL
                     SLFQSADYNVERTMKGIIYVDEIDK"
BASE COUNT          689 a         1049 c         1086 g          587 t
ORIGIN      
        1 cctgggccgt gatcatctcg atttcgcgat gacgtttgcg ttgtgtgttc cgcggaatcc
       61 cggggatagg cgatcgtctg attttcaatg cctcgacttg ctcgtcggtc agcttcacgt
      121 cgcggtacca ggtggccaga gtcgacttct tgaccgggat caggtccatg atctcgccgt
      181 aggacagccc gaggcggcgc aggtccttgc ccaactcgga tcgctcccat cgggacaggg
      241 tgtcccacga atcgattcgc tctcgaaatg cggcccccac cgtcatggtt cgaacatagg
      301 aatggggtgg gacagaaact gacgggggaa gtagcctgac accatcacgg gctgtggcgc
      361 agcttggtag cgcgcgtgct ttgggagcac gaggtcgtcg gttcgagtcc gaccagcccg
      421 acgaggcttt acaatgcctt gtcccgcggg tgtagctcaa tggtagagct ccagccttcc
      481 aagctggcgg tgagggttcg attcccttca cccgctcgaa tgggtggccc tcttggcaca
      541 gacctggccc tttgcccctg tagctcaatg ggatagagca gcggacttct aatccgcagg
      601 ttgggggttc gagtcctccc gggggcgcag aggaagaccc attcgagaca tggtggccgt
      661 agctcagtcc ggttagagca cctggttgtg gtccaggaag tcgcgggttc gaatcccgtc
      721 ggtcaccctt tcagagggcg ctcggaaaga gcgccctcgt actgggtagt cggtacctgg
      781 taccagtaca accaaccagg aagagcatgc caaccactgt caccgagacc gggcccttcg
      841 agcgtctggt cgtttttcaa ctcacagacg accagatcgc cgcaggcaag gccgccaccg
      901 cccgcaagct ctcccatgac ctcaagctca agggcttccg accggggaag gcccccctgc
      961 cggtggtgga agccgcggtg ggaccggccc gtctccgcag cgaggtgatc gacgacctgg
     1021 tcccaccggt cctcaccgac atcctcaacg acgaggagct gagaccggcg gtcacccccc
     1081 agctcgaggc gatgaacgac gtcgagggcg gagtcgaggt ggaagtccgg gtcacgctgt
     1141 ggccggtcgt cgacctgccc aactacaaaa accgagaagt cgaggtgacc tcgccggagg
     1201 tcaccccgga ggagttggag tctcagctgg agcggatgct cgagcagttc gccacggtgg
     1261 aggaggcgaa ccgtccggcg agaactggcg acttcgtctc ggtcgacgtg aaggcgacca
     1321 gcgaaggcga gtcggtcgaa ggggccagtg ccgccgacct cctctacgag gttgggtccg
     1381 gtctgctggt cgagggtgtc gacgagcgtc tcgaaggggc cgagccggga accgtcctca
     1441 cgttcgacgc ccccctgccc ggcgggttcg gtgaaagggc gggggaggag gtcagcttcg
     1501 agatcgtggt caacgaggtg aaggagcgga tcctgcccgc cctcgacgac gagtgggtcg
     1561 acgagaacac cgagttcgag accgtcgagg agttgcgggc cgagttggag gagcgtctcg
     1621 gcgaagccaa ggtccaggcc gtgtcccggc agttcgccga gagggcattg agcacgctgg
     1681 tcgaccaggt cgagatcgag ttgcccgacg ccctggtccg ctcggagatg gacgaccagc
     1741 ttcatcggtt cgtccatcgt ctcgaagaca acgagctaac cctcgacgac tacttccagg
     1801 cctcgggcat cgaccaggag cagttcctcg acgacctccg ccagcaggcc gagcgctcgc
     1861 ttcggaatca gctggtgctg gaggctgtgg ccaaggatgc cgggatcgag gtgacgcccg
     1921 atgacgtctc ctcggtgatc caggcggctg cggcgcagtc cggagacccg gtggcctatc
     1981 tcaacgcgtt caggcaaggc ggccgggaat tggcactggc cggtgatatt ctcagaaaca
     2041 gggcgttgga cgccatcctc tcggcagcaa acccggtcga tgaagagggc aacccggtcg
     2101 atttgaaact gaacatgaca gaggtcgagg ccgagatcgt cgaaggcgag ctggtcgggg
     2161 actacccggc gggcgagatc gtcgacgcag tggcggagga agaagaatga cagccgagaa
     2221 ccagttccag aactacgtga tccccaccgt cttcgagcag accagtcgcg gcgagcgcta
     2281 cttcgacatc tacagccgtc tgctcaagga tcgcatcatc ttcctcggca ccccgatcga
     2341 cgacaccatc gccaacctga tcatggccca gctcctccat ctcgaatccg aggacccgga
     2401 caaggacatc ttcctctaca tcaactcccc gggagggtcg atcacgtcct tgttcgccat
     2461 ctacgacacg atgcagtaca tcaagccaga cgtgaacacg gtttgcatgg gcatggccgc
     2521 gtcggcggcg gcggtgatac tggccggtgg cgcggagggg aagcgatacg ccctgcccca
     2581 tgcccgggtg atgctgcacc agccgcatgg cggtgctcag ggtcaggcct ccgacatcga
     2641 gatacaggcc aagctcatcg tgcagatgcg tgaacagctc aacgagatcc tcgcccacca
     2701 caccggccag gagtacgaca agatcgccct cgacacagaa cgcgactact ggatgctcgc
     2761 cgacgcagcc aaggaatacg gggtcatcga cggaattctc acccgtcggg agcttgcggc
     2821 ggtagcctcc aagtaacgga ggaggtccgg aactcgatgg cacgactcgg tgacggcggg
     2881 gagttgctca aatgcagctt ctgcgggaag tctcaaaagc aggtcaagaa gctgatcgca
     2941 ggccccggcg tctacatctg tgacgagtgc atcgatctct gcaacgagat catcgaggag
     3001 gagttctcgg tcaccgagga gatcacgttc accgagcttc ccaaaccggt ggagatcaac
     3061 tcctatctcg acgactacgt cgtcggccag gaagacgcca agaagaagct ctccgtcgcc
     3121 gtctacaacc attacaagcg gatcaggggc ggtgagatcc agcgtgatgt ggagctgcag
     3181 aagtcgaaca tcttgctcat cgggcccacc gggtcgggca agacccttct cgcccagacc
     3241 ctggcccggc aactgaacgt tcccttcgcc attgccgacg ccaccagcct caccgaggcc
     3301 ggctacgtcg gcgaggacgt cgagaacatc atcctctccc tcttccagtc cgccgactac
     3361 aacgtcgagc gcaccatgaa gggcatcatc tacgtcgatg agatcgacaa g
//
LOCUS       DASUTA010000205         4113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_837209, whole genome shotgun sequence.
ACCESSION   DASUTA010000205 DASUTA010000000
VERSION     DASUTA010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837209"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /gene="alc"
                     /locus_tag="VFZ80_04085"
     CDS             complement(<1..728)
                     /gene="alc"
                     /locus_tag="VFZ80_04085"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HEX6286639.1"
                     /translation="MIDLISERVGGEVVECNDEFFAEAVNLIKAEEPVWREDLYTDRG
                     KWMDGWETRRRRDPGHDWCVVSLGIPGLVRRVIVDTSYFTGNYPEEFSLDVSPGSDIW
                     SELIPRTQLQGDSKASFDVESPHRVTAVRLNIYPDGGVARLRVEGDPIPSMQIVCPDG
                     PADLVDARLGGEWLEASDYHYSPPSNLLMPTESAGMWDGWETRRRRGPGHDWATFRLG
                     LPGLVESVVVDTTYFKGNCPGWVSV"
     gene            complement(725..1936)
                     /gene="allB"
                     /locus_tag="VFZ80_04090"
     CDS             complement(725..1936)
                     /gene="allB"
                     /locus_tag="VFZ80_04090"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HEX6286640.1"
                     /translation="MISIRSTRVVLPDGMRPATVRIDNGLFVDIGEGIADRDYGDHVL
                     MPGLVDSHVHVNEPGRTEWEGFATATRAALAGGTTTIVDMPLNSVPPTTTVAALEEKR
                     TAAGGKLSTDVAFWGGLVPGSESEVGRLTDAGVCGFKVFLTDSGVPEYPPMTVAALEE
                     IRTHLPLLIHAEATELLGEPGPSYSDYLASRPPEAESEAIRRVSGLDPPIHILHVSSA
                     AGVEAVGSAVGVSGETCPHYLIFTDADVTGTEFKCAPPIRDAENREALWEGLESGMLA
                     MVVSDHSPAPPAMKRGDFATAWGGIASVQLRLCATWTGAARRGVGLPALAGWLAAAPA
                     HLAGLDDRKGSIAPGKDADFVVFDPDGVTKVTGADLHHRHALTPYEGMRLRGEVVETS
                     LGSPARMLDRR"
     gene            1979..2521
                     /locus_tag="VFZ80_04095"
     CDS             1979..2521
                     /locus_tag="VFZ80_04095"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX6286641.1"
                     /translation="MTTAYNPDRTINYAFLLGGLTALIFGVILLVRQDAAIGIVALLL
                     GLWWLIQGAFLLFSVFIDRTDMWWKLIIGLIGVTAGLIVLANPVQSAEFLGSAMAVIL
                     GILAIINGIVAVIGGFRGGGFPAILFGIVTAAIGVLLLLYPEGSFTAFVTIIGFILMV
                     QGAAAIVLSIVAKPDIAVTE"
     gene            2640..3917
                     /locus_tag="VFZ80_04100"
     CDS             2640..3917
                     /locus_tag="VFZ80_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6286642.1"
                     /translation="MADLLSRHNDVLAPVIAFSTKIHAESADGLWITDPDGNRWADFA
                     CGTAVTNLGHRHPAVVEAARAQLDKLIHSGMVVHFESLVEAAERLRDITPEGIEKFGF
                     ANSGAEAVEASVKMAKYTTKRQGIVTFRGGFHGRTMGSVAYTTSKAKYRDGYHPVLGS
                     VFVTPFPHPYRWGVDADTAVEMSLHELEMMFKHVVSPANIAAFLVEPVQGEGGYYPAP
                     PRFLEQLRAIADENGILLIYDEVQTGFGRTAEWFAADHFEAKPDIVALGKGIANGFPL
                     SAYGAGKEIIDNWPPGSHGTTYGGNPVAAAASVAVMDTMRGLLPHARDLSKRAFERFH
                     QLADKHETVGDVRGLGLMIGVEFVEDRDSRAPDAKAFQHVAAHAFDNELIIIECGPDG
                     NILRFIPPLITTMDELDWAIDLVDEGLESWEAN"
BASE COUNT          750 a         1359 c         1261 g          743 t
ORIGIN      
        1 accgacaccc accccgggca gttccccttg aagtaagtgg tgtcgaccac gacagactcg
       61 accaaaccgg ggagaccgag ccgaaacgtg gcccaatcgt ggcccggtcc gcgacgccgc
      121 ctggtctccc acccgtccca catccccgcc gactcggtgg gcatgagcag gttcgaaggt
      181 ggtgagtagt gatagtccga cgcctccagc cactcgccac cgagtctggc atcgaccagg
      241 tcagccggcc catcgggaca gacgatctgc atgctcggga tcggatcgcc ttcgactcgc
      301 agacgagcca ccccgccatc gggatagatg ttcaggcgta ccgcggtgac gcgatgcggg
      361 gattcgacgt cgaacgaggc cttggagtcg ccctggagtt gggtccgggg gatgagctcg
      421 ctccagatat cactgcccgg cgagacgtcg agggagaact cttctggata gtttccggtg
      481 aagtacgacg tgtcgacaat gacccgcctg accaggcccg ggatccccag tgagaccaca
      541 caccagtcgt gaccgggatc gcgtcgtcgg cgggtctccc agccgtccat ccacttgccg
      601 cggtcggtgt acaggtcttc tctccacaca ggttcctcgg ccttgatgag attgaccgcc
      661 tcggcaaaga actcgtcgtt gcactcgacg acctcgccac cgacccgttc cgaaatgaga
      721 tcgatcatct gcggtccagc atcctcgcag gtgatcccaa cgatgtctca acgacctcgc
      781 cccggagccg catcccctca tagggagtga gtgcatgacg atggtgcaga tcggcgccgg
      841 tcaccttggt gacaccgtcg gggtcgaaca cgacgaagtc ggcgtccttg cccggcgcta
      901 tcgacccttt ccggtcgtcg agcccggcga gatgagctgg cgccgcggcg agccatccgg
      961 ccagagcagg tagcccaacc ccacgccggg ccgcccccgt ccacgtggca cagagccgaa
     1021 gttgaaccga ggcgatgcca ccccacgccg tggcgaagtc gccccgcttc atggccggtg
     1081 gggcaggtga atggtcggag accaccatcg ccagcatccc cgactcgaga ccctcccaga
     1141 gagcctcgcg attctcggcg tcgcgtatcg ggggtgcaca cttgaactcg gtgccggtca
     1201 cgtcggcgtc ggtgaagatg aggtaatggg ggcaggtctc cccgctgaca ccaacggccg
     1261 accccaccgc ctccaccccg gcagccgaag agacatggag gatgtggatc ggagggtcga
     1321 gcccactgac acgtcgtatc gcctctgact ccgcttccgg tgggcgggaa gccaggtaat
     1381 ccgagtacga cggcccgggt tctcccaata gctcagtcgc ctcggcatgg atcaggagag
     1441 gcaggtgggt ccgaatctct tccagagccg ccactgtcat aggcgggtat tcgggcaccc
     1501 cggaatcggt gagaaacacc ttgaacccgc agacaccggc gtcggtcaat ctcccgacct
     1561 cactttcgct gccaggaacc aaacctcccc agaatgcgac gtccgtggac agctttcccc
     1621 cggcagccgt ccgtttctcc tccaaagcag cgaccgtcgt agtaggcggc actgagttca
     1681 gcggcatatc gacgatcgtg gtcgtgcccc cggcgagcgc tgcccgggtc gcggtggcga
     1741 acccctccca ctcggtgcga cccggctcgt tgacatggac atgtgaatcg acgaggcccg
     1801 gcatcaacac atggtcgccg taatcgcgat cggcgatgcc ctcgccgatg tcgacgaaca
     1861 ggccgttatc gatcctgacc gtcgccgggc gcatgccgtc ggggaggacg acgcgagtgg
     1921 atcgaatcga gatcatcctg gccaacctac cggcgctcga tcggggtagt ttgttgtcat
     1981 gacgaccgca tacaaccctg atcggacaat caactacgcg ttcctgctcg gtgggctgac
     2041 tgctctgatc ttcggcgtga tcctcctggt ccgtcaggat gcagcaatcg ggatcgtcgc
     2101 cctcctgctc gggctgtggt ggctcattca gggcgcgttc ctgctcttct cggtcttcat
     2161 cgaccgcacc gacatgtggt ggaagctgat cattggcctc atcggagtca ccgccggtct
     2221 gatagtgctt gccaaccctg tccaatcggc tgagttcctg ggcagcgcca tggccgtcat
     2281 actcggaatc ctcgccatca tcaacggcat cgtggcggtg atcggaggct ttcgcggcgg
     2341 tggcttcccg gccatcctct tcggcatcgt gactgctgcg attggtgttc tcctccttct
     2401 ctatccggag gggtcattca ccgccttcgt gacgatcatc ggcttcatcc tcatggtcca
     2461 gggtgccgcg gcgatcgtcc tgtcgatcgt ggccaagccg gacatcgcag tgaccgagta
     2521 acgtccgatt cgtgaacaac ccgctgccgc gggccgctag acgaggcgtt tacgccgttc
     2581 catagggaga ctcccggaag cgaacgtaac gcccgctatc ggtcccaagg agtcaaaaaa
     2641 tggccgatct tctctcacgt cacaacgacg tcctagctcc agtcatcgct ttcagcacaa
     2701 agatccacgc cgagtcagcc gacggcctct ggatcaccga tcccgacggc aatcgttggg
     2761 cggacttcgc ctgtggcacc gctgtcacca acctcggcca ccgccatccg gcggtggtcg
     2821 aggcggccag ggctcagctc gacaagctga tccactccgg aatggtcgtc cacttcgagt
     2881 cactcgttga agcggcggag cgtctgcgcg acataacccc ggaggggatc gagaagttcg
     2941 gtttcgccaa ctcaggggcc gaggcagtcg aggcgtctgt gaagatggcc aagtacacga
     3001 cgaaacgcca gggcatcgtc actttccgcg gcggcttcca cgggcggacc atgggatcgg
     3061 tcgcctacac gacttcgaaa gccaagtaca gggacggcta ccaccccgtg ctcggctcgg
     3121 tcttcgtcac gccgttccca catccctatc gatggggcgt cgacgccgac accgccgtcg
     3181 agatgtcgtt gcacgagctg gagatgatgt tcaaacatgt cgtctcccca gccaacatcg
     3241 ctgcgttcct cgtcgagccg gtccagggtg aaggcggcta ctacccggcg ccgccacgct
     3301 ttctcgagca gttgcgagcc atcgccgacg agaacgggat cctcctgatc tacgacgagg
     3361 tgcagaccgg tttcgggcga actgccgagt ggttcgccgc cgaccatttc gaggccaagc
     3421 ccgacatcgt ggccctcggc aagggcatcg ccaacggatt cccgctctcg gcctacggcg
     3481 ccggcaagga gatcatcgac aattggccac ccgggtcgca cgggaccacc tacggcggca
     3541 acccggtggc ggccgcggcc agcgtcgcgg tgatggacac gatgaggggg ctactgccac
     3601 atgcccgcga cctctcgaag cgcgcattcg agcgattcca ccagttggcc gacaagcacg
     3661 agaccgtcgg cgatgtgcgt gggctcggcc tcatgatcgg tgtcgagttc gtggaggata
     3721 gggactcgag ggcgccggac gccaaggcct tccagcacgt tgcggcccat gctttcgaca
     3781 acgagttgat catcatcgag tgcgggcccg acgggaacat ccttcgattc atcccgccac
     3841 tcatcaccac gatggatgag ctcgactggg cgatcgatct cgtcgacgag ggtctggaga
     3901 gctgggaggc caactagccc ggaacttctc gggattccgg cgcgtctaag cgagatgaac
     3961 cggaagtcag tcatagcctt tttgtctgtc gcgatcctcc tcgccgcctg cggcgggggc
     4021 ggaggagcct cgagcgacgt cgggcctgac gaagcgctga tccgaatctc gtcagagggt
     4081 ggattcgtcc cggtcgagtt tgcgcttggc acc
//
LOCUS       DASUTA010000206         2821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1357101, whole genome shotgun sequence.
ACCESSION   DASUTA010000206 DASUTA010000000
VERSION     DASUTA010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357101"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..184)
                     /locus_tag="VFZ80_04105"
     CDS             complement(44..184)
                     /locus_tag="VFZ80_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286643.1"
                     /translation="MTDNAPKPGADDDFVFALEPAQLGAVVALLIAVIVLWRVARKDR
                     KV"
     gene            387..500
                     /gene="rpmJ"
                     /locus_tag="VFZ80_04110"
     CDS             387..500
                     /gene="rpmJ"
                     /locus_tag="VFZ80_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006042225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX6286644.1"
                     /translation="MKVRPSTKKMCEKCRIIRRRGRVWVICSNPRHKQRQG"
     gene            505..882
                     /gene="rpsM"
                     /locus_tag="VFZ80_04115"
     CDS             505..882
                     /gene="rpsM"
                     /locus_tag="VFZ80_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX6286645.1"
                     /translation="MARIAGVDIPRDKRLEISLTYIYGIGNTTAQKICADTDIDPDTR
                     VRSLTDDEVARIRRYIETNFKVEGDLRREVQQNIKRKIEIGSYQGIRHRRGLPVRGQR
                     THTNARGRKGRRVAIAGKKKVTK"
     gene            882..1286
                     /gene="rpsK"
                     /locus_tag="VFZ80_04120"
     CDS             882..1286
                     /gene="rpsK"
                     /locus_tag="VFZ80_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX6286646.1"
                     /translation="MTSRQQGGGAKRVRRRERKNISHGQAHIKASFNNTIINITDPTG
                     ATVAWTSGGSVGFKGSRKSTPYAAQVAAEEAARRAGEHGIRKLDVIVSGTGSGRDTAV
                     RTLQNMGMEVTWVKDVTPMPHNGCRPKKRRRG"
     gene            1450..2055
                     /gene="rpsD"
                     /locus_tag="VFZ80_04125"
     CDS             1450..2055
                     /gene="rpsD"
                     /locus_tag="VFZ80_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX6286647.1"
                     /translation="MARFTGPKCKACRRARTQLCQSKRCAVERRPYPPGEHGRGRVRE
                     SDYQVQLREKQKLRTMYGVLERQFRRYYEEAARRPGITGENLLQMLEQRLDNVVWRSG
                     LAATRPQARQLVNHGHFRVNGKKVDIPSYQVRPGDVISPKDRSKDLIVIQHSVDTQDR
                     LVPDWIVVSTDDRQITVNDHPRRDQIDTDINEQLIVELYSK"
     gene            2069..>2821
                     /locus_tag="VFZ80_04130"
     CDS             2069..>2821
                     /locus_tag="VFZ80_04130"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX6286648.1"
                     /translation="MIVQRPQIEIEEVGENRSRFVVEPLEPGFGYTLGNTMRRTLLSR
                     IPGAAITSIQIEGVEHEFSTVAGVVEDVVDIILNIKRIVIQMDGFDEQTVYLSAKGAG
                     DVTAADLKLPAGVEVVNPDAHIATLSASGRLEMEMTVAPGVGYRTADANKSGSTHIGV
                     IPVDSIFSPVRKVAYRVENTQVGQMTNYDKLILDVETNGAIEPKDAVSSAGKTLGELM
                     EIFADVGQGEGLELVDIVPEASGSPDLELPIEA"
BASE COUNT          586 a          926 c          835 g          474 t
ORIGIN      
        1 aggagctcgc cagtcgataa ggggcacacg tgaccccggc tgctcagacc ttgcggtctt
       61 ttctcgcaac ccgccagagc acgatcacgg cgatcaacag agcaaccacg gccccgagtt
      121 gagctggctc caatgcgaac acgaagtcgt cgtctgcacc cggtttcgga gcattatcgg
      181 tcatgtcccc aacgatacgc gcccgacttg agcaaccaca cacccctcgt tatcatttga
      241 gggtctttac cgctctttct gtggccgaaa ggcggttagt agccagggca ccgtcgcccg
      301 caaccacact gacgctccgg ttgcgattgt ttggcgccct ggcattgcac gcgttcgcgg
      361 gcaccacaaa gaattggaga aacacgatga aggtgagacc ttccacaaag aagatgtgtg
      421 agaagtgtcg gatcatccgg cgtcgtggcc gcgtctgggt gatctgctcg aacccccgac
      481 acaagcagcg acagggttga tctgatggca cgaattgcag gagtcgacat tccccgggac
      541 aagcgtctcg agatctcgct cacctatatc tacggaatcg gcaacacgac ggcgcagaag
      601 atctgcgccg acaccgatat cgatcccgac acacgcgtcc gctctctgac cgacgacgag
      661 gtcgctcgaa tccgtcgcta catcgagacc aacttcaagg tcgaaggtga cctgcgccgc
      721 gaggtccagc agaacatcaa gcggaagatc gagattggca gctaccaggg catccggcat
      781 cgccgcggtc tcccggtgcg cgggcagcgg actcacacca acgcccgggg ccgcaagggc
      841 cgccgtgtcg ccatcgccgg caagaagaaa gtgaccaagt agtgacgtcc cgacagcagg
      901 gtggcggcgc caagcgtgta cgtcgccgtg agcgcaagaa catctcgcac ggccaggccc
      961 acatcaaggc cagtttcaac aacaccatca tcaacatcac cgaccccaca ggcgccaccg
     1021 tcgcctggac ctccggcggc tccgtcggat tcaagggctc gcgcaagtcg accccctacg
     1081 cggcgcaggt cgcggcagag gaagccgccc gtcgtgctgg tgagcacgga atccgcaagc
     1141 tcgacgtcat cgtcagcggc accggatccg gtcgcgacac cgcggtgcgc accctgcaga
     1201 acatgggcat ggaggtcacc tgggtcaagg acgtcactcc gatgccgcac aacgggtgtc
     1261 gtcccaagaa gcggaggcgt ggatagtgcc taccctcgca acactccccg gcctcacagc
     1321 ggccagtgac ctcgccagcg gcgtcctcgt cctagacgca ccgtcccggt gcgccttcgt
     1381 cctgcgtcct ggctggacaa cgcccctgcc tcgctctgag gctcgacagt gtcacgagag
     1441 caggcactga tggcccgttt cacaggaccg aagtgcaagg cgtgccgacg agcccgcacc
     1501 cagctctgtc agtcgaagcg ctgtgccgtc gagcgtcgcc cctatccgcc tggagaacat
     1561 ggtcgtggcc gggtccggga gagtgactat caggtccagc tccgcgagaa gcagaagctg
     1621 cgcaccatgt atggcgtgct cgaacgtcag ttccgccgct actacgaaga ggcggcgcgc
     1681 cgtcccggca tcaccggcga gaatctcctc cagatgctgg agcagcgtct cgacaacgtc
     1741 gtatggcgct cgggtctggc cgcaacccgc ccccaggccc gtcaactggt caatcatggc
     1801 cacttccggg tcaacggcaa gaaagtggac atcccgtcct atcaggtgcg cccgggtgat
     1861 gtcatctcac caaaggaccg ctccaaggac ttgatcgtca tccaacattc cgtcgacacc
     1921 caggatcggc tggtcccgga ctggatcgtc gtcagcacgg acgatcgtca gatcaccgtc
     1981 aacgatcatc cccgccggga tcagatcgac accgacatca acgagcagtt gatcgtcgag
     2041 ctctactcga agtgaggtaa cccacttgtt gattgttcag cgaccccaaa tcgaaatcga
     2101 ggaagtcggc gagaaccgat cgcgcttcgt ggtggagcct ctcgagcctg gcttcggtta
     2161 cacgctcggg aacacgatgc ggcggacgct tctgtcccgc atccccggcg ccgccatcac
     2221 ttcgatccag atcgaaggag tcgagcacga gttctcgacg gtcgccggtg tggtggaaga
     2281 tgtcgtggac atcatcctca acatcaagag gatcgtcatc cagatggacg gtttcgacga
     2341 gcagaccgtc tacctttcgg ccaagggagc cggtgacgtg accgcggcag acctcaagct
     2401 gcccgccggt gtcgaggtgg tcaatccaga cgctcatatc gccacgctct ccgcctcggg
     2461 ccgtctcgag atggagatga cggtggcgcc gggcgtggga taccgcaccg cggatgccaa
     2521 caagagcggg agcacccaca tcggtgtcat cccggtcgac tccatcttct cacctgtgcg
     2581 caaggtcgcc taccgggtgg agaacaccca ggtcgggcag atgaccaact acgacaagct
     2641 catcctcgat gtcgagacca atggggccat cgagcccaag gacgcagtct cttctgcagg
     2701 caagacgctc ggcgagctca tggagatctt cgccgatgtc ggacagggtg aaggcctcga
     2761 gctggtcgac atcgttcccg aggcttcggg ctcgcccgat ctcgagttgc ccatcgaggc
     2821 a
//
LOCUS       DASUTA010000207         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_837910, whole genome shotgun sequence.
ACCESSION   DASUTA010000207 DASUTA010000000
VERSION     DASUTA010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837910"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFZ80_04135"
     CDS             <1..273
                     /locus_tag="VFZ80_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX6286649.1"
                     /translation="ECLGCTFQGTAAEFPDRDHPGHQVAVAHKDAVRQRFTALARQAA
                     LTEPEALGDQLLLLMDGAWVAARMYGSDNPAAGVAQAARRLIDTHR"
     gene            complement(509..781)
                     /locus_tag="VFZ80_04140"
     CDS             complement(509..781)
                     /locus_tag="VFZ80_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5661 family protein"
                     /protein_id="HEX6286650.1"
                     /translation="MFTTEDAAAALAAAGIDISGERYELKDVRDGMNVELEHGTRFPD
                     LDVSGDDPVITVKIALAHLREFPDYYERLAVMERDREAAWGATAGH"
     gene            complement(981..>2099)
                     /locus_tag="VFZ80_04145"
     CDS             complement(981..>2099)
                     /locus_tag="VFZ80_04145"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX6286651.1"
                     /translation="LSWVLDGAAETAEVTAFRSRVKKISPTVVYISIDGEVAGAFVFD
                     DQVRGDAVYTIRALRRTGVEKVVMATGDHPLVARSVGLAIGVDDVLAEATPEEKVLLL
                     EDLQRSGTTAMVGDGINDAPALAAADVGVAMGARGATASSEAADVVLVVDRLQRLVDA
                     LGIAQRTRRIALGSVVIGMGLSALAMTAAAFGLLVPIAGALVQEGIDVVAILNALRAL
                     GGETPTQSGPRLPAELSEELRVEHERLIPQLDRLRQVADALDMQSPSEGLRSLHEIEG
                     FLVEEILPHEANDERVIYPQMGEILPGDDPLATMSRGHREIFHLVEVLQRQVADLPAK
                     GIEPADITDLRRTLYGLHAILRLHFDQEEELYASLERG"
BASE COUNT          392 a          715 c          617 g          375 t
ORIGIN      
        1 gaatgtctcg gctgcacctt ccaaggcacc gccgccgagt tccccgaccg cgatcacccc
       61 ggccatcagg tggccgtagc ccacaaggac gccgtgaggc agcgattcac cgctctcgca
      121 cggcaggccg ctctcaccga gcccgaagcc ctcggcgatc agctactgct gttgatggat
      181 ggcgcctggg tcgccgcccg catgtacggg agcgacaacc cggccgccgg cgtcgcccag
      241 gctgcaagac ggctcatcga tacccaccgt tgaccggcga acacgttgtc ttgcgccccg
      301 catcctgtcg agaagcctga cgaccacccg agctaacccg ccgcggtcag gtggattcgc
      361 taacagcgcc gtactcgtcc tccagcgggc tagcgagacc gtcaccaagc accatccgac
      421 cgccgcacag gtgatcgacc gtcaccgcta tcccgcgcga cgctcgatgc gaaggtcggc
      481 atctccgctt ctgggctgtc gtgggagatc aatgcccggc cgtcgccccc caagcagctt
      541 cccggtctcg ctccatgacc gcgagacgct cgtagtagtc cggaaactca cgaaggtgag
      601 cgagggcaat cttcaccgtg atcacagggt cgtcgccact cacgtcgagg tctgggaacc
      661 gggtgccgtg ctccaactcc acgttcatac catcccgaac atccttcaac tcataccgct
      721 caccggagat gtcgataccg gctgcagcca aagccgcagc ggcatcctcg gtcgtgaaca
      781 tcactgctcc tatctggaaa cttctgtcta acactcgatc aagccaccgc gttcattgtt
      841 ggaccgtgtg acgcggttcc accagaggca aaggaccttt gtgaggagac atcggtcttc
      901 gcagcgtgcc ggagatgccg tgtccacttc ggtcgttacg gcggattcag gacatacccg
      961 tggttcggag cgtgcgacgt tcaacctctc tctagcgagg cgtagagctc ctcttcttgg
     1021 tcgaagtgga gtctgagaat ggcgtggagg ccgtaaagcg tccttcgcag gtctgtgatg
     1081 tcggccggtt cgatgccctt tgccggcagg tcggcaactt gacgctgcaa cacctcgacc
     1141 aggtggaaga tctctcgatg accccggctc atggtcgcca gtggatcatc gcccggaaga
     1201 atctcgccca tttgcgggta gatcactctc tcgtcgttgg cctcatgagg aaggatctcc
     1261 tcaacgagga agccctcgat ctcgtgcagc gagcgcaggc cctcggaggg actctgcatg
     1321 tcgagggcgt cggccacctg acgcaatctg tcgagttgtg ggatcagtcg ctcgtgttcg
     1381 acgcggagtt cctccgaaag ctcagcaggg agccggggcc cggactgagt cggggtctct
     1441 ccgccgaggg cccgaagggc gttgaggatg gcgacgacgt ctatgccttc ctgcaccaga
     1501 gcaccggcta tcggaaccag aaggccgaat gcagctgcag tcatcgccaa tgctgacaga
     1561 cccataccga tgacgacgct tcccagggcg attcgacggg tgcgctgggc tatcccgagg
     1621 gcatcgacca gcctctggag acggtcgaca accagaacca catccgccgc ctccgaggac
     1681 gctgtcgccc cccgagcccc catggcgacc ccgacgtcgg cggccgccag tgcaggagca
     1741 tcgttgatgc cgtcaccaac catcgccgtg gtcccgctgc gctgcaaatc ctcgagaagg
     1801 agcaccttct cttcaggtgt cgcttcggcg agcacatcgt cgacacctat ggccagtccc
     1861 accgaccgcg ctaccagggg atgatccccg gtcgccatca ctaccttctc cacaccagtg
     1921 cggcgaaggg cccggatcgt gtacacggcg tctcctcgca cctggtcgtc gaagacgaat
     1981 gccccggcga cctcgccatc gatcgagatg tacaccacag tcggagagat cttcttgacg
     2041 cgactccgga atgcagtgac ctcggccgtc tcggcggctc cgtcgaggac ccacgagag
//
LOCUS       DASUTA010000208         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_321458, whole genome shotgun sequence.
ACCESSION   DASUTA010000208 DASUTA010000000
VERSION     DASUTA010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321458"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VFZ80_04150"
     CDS             <1..393
                     /locus_tag="VFZ80_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6286652.1"
                     /translation="RPAMRVVTHVTHVQRLDAGERPSYGRVRELERASTVVTAPIGYA
                     DGFPRLLTQEGSVLVGGMRFPLAGTVTMDQIVIDVGDEDVDVGEEVTLLGAQGNEEIT
                     ADEWADALGTISYEVVCSIGPRVSRRYT"
     gene            390..1277
                     /locus_tag="VFZ80_04155"
     CDS             390..1277
                     /locus_tag="VFZ80_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017870530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX6286653.1"
                     /translation="MSETITAVPGVRVGHWTDSNALTGCTVVVLPEPNVAAGEIRGGA
                     PGSRETALLQPGMSVQQAQAFLLTGGSAFGLAAADGVVRGLVEDDRGHDTGVGVKVPI
                     VPAAVIFDLMHGDPSVRPGPDEGYAAYRSASEAPVESGLIGAGTGATAAKWRGLENMI
                     PGGLGSASELAGDAIVGVLAVLNPVGDVFTLEGESLTGGDHVPGPPQLAPSDLTNTTL
                     VVVATDADLPRVGLNRLIVRSHDAIAACVRPSHTRYDGDAVFAASVGGVTADHDVLGE
                     AVFVATARAIENAVRAANG"
     gene            1306..1914
                     /locus_tag="VFZ80_04160"
     CDS             1306..1914
                     /locus_tag="VFZ80_04160"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX6286654.1"
                     /translation="MSPASPAFVELRDAALVCTACRLAETRTNVVFGVGDPDARLMLV
                     GEAPGKNEDLQGEPFVGAAGHLLDELMGSIGISREEAYIANVLKCRPPGNRDPQQDEI
                     DSCKGYLREQIRMIHPDVVVTLGNFATKLLVPTEVGITRMRGQAIEWWLGATLIPTFH
                     PAAALRGRPSVKEQMQEDFRLVRQVLERTAQQVDPNQLDLGR"
     gene            1911..>2109
                     /locus_tag="VFZ80_04165"
     CDS             1911..>2109
                     /locus_tag="VFZ80_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX6286655.1"
                     /translation="MIEIRCPRPDDTEALGSRIASNLRPGDVVVLAGGLGVGKTLFTR
                     GIATGLGVDEPVVSPSFVLVRQ"
BASE COUNT          371 a          654 c          710 g          374 t
ORIGIN      
        1 cgcccagcaa tgcgcgtcgt cacccatgtc acccatgttc aacgattgga tgcgggggag
       61 cgaccttcct atggacgggt gcgagagcta gagcgagcgt cgacggtggt gacggccccc
      121 atcggctacg ccgacggctt cccgaggttg ctcacgcagg agggctcggt cctggtcggc
      181 ggaatgaggt ttccactcgc cggaaccgtc accatggatc agatcgtgat cgatgtcggc
      241 gatgaggacg tcgacgtcgg agaggaggtg actctgctcg gggcacaggg caacgaggag
      301 atcaccgccg acgaatgggc agatgcattg ggcacgatct cctacgaggt ggtttgctcg
      361 atcgggcctc gggtatcgag gcgctacaca tgagcgagac gatcaccgcg gttcccgggg
      421 tccgagtggg gcactggacg gactccaacg cactgacagg atgcactgtc gtcgtgcttc
      481 ccgagccgaa cgtagccgcc ggggagatca ggggcggtgc tccaggaagc cgggaaacgg
      541 cgctgcttca gcccgggatg agcgtccagc aagctcaggc attcctgctc acaggtggca
      601 gcgcgttcgg gctcgccgca gccgacggtg tcgtgcgtgg attggtcgag gacgaccgcg
      661 ggcacgacac cggtgtcggc gtcaaggtcc caatcgtgcc tgcagccgtc atcttcgatc
      721 tgatgcatgg cgacccctcg gtgcggccgg gcccggatga gggttacgcc gcttatcgca
      781 gcgcatcgga ggctcctgtg gaatcgggac tgatcggtgc cggcaccggt gccaccgcgg
      841 ccaagtggcg cggtctcgag aacatgatcc cgggtggtct cggctccgca tcggaacttg
      901 ccggagatgc gatcgtggga gtgctagccg tcctcaaccc ggtgggcgac gtcttcaccc
      961 tcgaaggcga gtcactcacc gggggtgatc acgtgccggg acctccacag ttggctcctt
     1021 cagacctcac caacacgacc ctcgtggtgg tggccaccga tgcagatctc cctcgagtcg
     1081 ggttgaatcg gctgatcgtc aggtcacacg acgccatcgc cgcctgcgta cggccatcgc
     1141 acacgcgata tgacggtgac gctgtctttg ccgcttccgt cggcggagtg accgctgatc
     1201 acgatgtgct gggggaggcc gtcttcgttg ccacggctcg ggcgatcgag aatgccgtgc
     1261 gagccgccaa cgggtagccg gactgtttgc cacaatggag cccaggtgag cccggccagc
     1321 cctgccttcg tagaactgcg cgatgccgcg ctcgtgtgca cggcgtgccg cctcgctgag
     1381 acacggacca atgtcgtctt cggtgtcggc gatccagacg cccgtctgat gttggttggg
     1441 gaggcgcctg gaaagaatga agacctacag ggtgagcctt tcgtcggggc cgcaggccac
     1501 cttctcgacg agttgatggg ttccatcgga atttcccggg aagaggccta catcgccaac
     1561 gtcctgaagt gccgaccacc cggcaatcgc gacccacagc aagacgagat cgactcgtgc
     1621 aagggctatt tacgcgagca gatacgcatg atccatcccg atgtggtggt gaccttgggc
     1681 aacttcgcca ccaagttgct agtccccacc gaggtcggga tcacccgcat gcgcggccag
     1741 gcaatcgagt ggtggctcgg cgccactctg ataccgacct tccacccggc agccgccctt
     1801 cgcgggcgcc cttcggtgaa ggagcagatg caggaggact tccgactcgt gcgccaggtt
     1861 ttggagcgga cggcgcaaca ggtcgatccg aaccagttgg atctgggccg atgatcgaga
     1921 tccgatgccc acggcccgat gacaccgaag cactggggag ccgtatcgca tccaacctgc
     1981 gaccgggcga tgtcgtggtt ctcgccggtg gcctcggggt agggaagacc ctgttcaccc
     2041 gggggatagc cacggggctc ggtgtcgacg aaccggtagt cagcccatcg ttcgtcctcg
     2101 tccgccagt
//
LOCUS       DASUTA010000209         2854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_449104, whole genome shotgun sequence.
ACCESSION   DASUTA010000209 DASUTA010000000
VERSION     DASUTA010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449104"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VFZ80_04170"
     CDS             <1..458
                     /locus_tag="VFZ80_04170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6286656.1"
                     /translation="VDEAGIEAYRSVMARCGEGTLDRNDRYYWEGCGPDNWAGQMTEY
                     LDPEDAPMWLLGYARGEPVGYVAVVRDEELVSTIAHIGVVPEHRGHGYAGDLLAAATA
                     VARRAGLARMLSDVDVFNRPMAETMRRAGHAEHPDWHVWAFRAQWPGLT"
     gene            1002..1478
                     /locus_tag="VFZ80_04175"
     CDS             1002..1478
                     /locus_tag="VFZ80_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX6286657.1"
                     /translation="MSTIKQAASEFLSNRRIAVTGVSRNPAGHGSNTVYDRLKARGYE
                     VFPVNPNAAEIDGDQCFSDIRSIPGGVDAVVIATSPERAMATMEECVALNIGHVWMHR
                     AFGDGSVSPEAAAWGRDNGIQVIDGGCPLMFDPVADGGHKAMRVVFTLTGKVPRKV"
     gene            1703..2071
                     /locus_tag="VFZ80_04180"
     CDS             1703..2071
                     /locus_tag="VFZ80_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286658.1"
                     /translation="MTEVTVRVPLEENMDLLRNTARACLGFTSPEGPRIEPAALRYRR
                     DRYLVGLDEGASVPDEGAEVVLVVDEGRLFFELRAVYVRGHATYARERSEGERTWIEV
                     VPTKITSWDYGRMRLDHGPD"
     gene            2058..2525
                     /locus_tag="VFZ80_04185"
     CDS             2058..2525
                     /locus_tag="VFZ80_04185"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6286659.1"
                     /translation="MVPTDPDVQEILKGCMVARLVTMSSSGRPHVNPLYFVWHDGKIH
                     LGTTTRTLAARNVVAHPMVQLLLDVESEPSRGRMVRIRGEAVLLTDPETLREYTRRDA
                     LKYFRSWNSLLMSLTHLRQLLLTRRYLSSDDPANTHSVIEIAPTDVELLTSTT"
     gene            2664..>2854
                     /locus_tag="VFZ80_04190"
     CDS             2664..>2854
                     /locus_tag="VFZ80_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286660.1"
                     /translation="MPMIVLLVLLFVASCSAEPEVSREEMVQHIQTKATAFSDLSLSS
                     EILEDLASHRVVVLGETHRL"
BASE COUNT          539 a          860 c          934 g          521 t
ORIGIN      
        1 ccgtggacga agcgggcatc gaggcctacc ggtcggtgat ggcgaggtgc ggggagggaa
       61 ccctcgatcg caacgaccgc tactactggg agggatgcgg tcccgacaac tgggcaggcc
      121 agatgaccga gtacctcgac cccgaggacg ctccgatgtg gttgctcggc tatgcgagag
      181 gcgaaccggt tggatatgtg gcggtcgtcc gcgacgagga actggtctcg acgatcgccc
      241 acatcggcgt cgtcccagag caccggggcc acggttacgc cggcgatctg ctggccgcgg
      301 ccaccgccgt agcgcgacga gccggcctgg cccggatgct gtcggatgtc gacgtgttca
      361 accggccgat ggcagagacc atgcgccggg ccggtcacgc cgagcacccc gactggcacg
      421 tctgggcctt ccgggcacaa tggccagggc tcacctgagg acgacgattc gcggccaacc
      481 gacgtcgacg acgccgatcg acgcctccag gccggaccac aggttggcga tgcctctcca
      541 ctccctgatc cggccgactc actgacctcc tcagacctcc atcctcgtgg tccacttcca
      601 gtggaccgag gctgtgcgac gctcagtgag cgaagagact cgatcggtca ggccgcgccg
      661 acgcagggcg cagccgaccg atgttgctgc ctgcgtcctg gagggggacg gccgaggttc
      721 cctgtgagta cccgccccat cgacgcccat caccttttct ctgctgtcct ccgattcccg
      781 attgtggaga tcaacatggg gctaccgctt gaggagaatg gccggagtca ggagagacct
      841 gctgcggcca cagaagatcc ggtgttgtcg ctgatcgagg gagaaggggt cattggaccc
      901 tattccgggc gtcgtatacc agatggtctg gtgtcgaggc gatgagtccg cctggtccgc
      961 atgtgacacg tgatcggtgg gcgagaagga aggaggtacc gatgtcgacg atcaagcagg
     1021 cagcatcgga gtttctgagc aaccgccgga tcgccgtgac cggcgtttcc cgcaacccgg
     1081 caggccatgg ctcgaatacg gtctatgacc gtctcaaagc ccggggttat gaggtgttcc
     1141 cggtcaatcc gaacgctgcg gagatcgacg gcgaccagtg cttctcggac atccgatcga
     1201 ttccgggagg tgtcgatgcg gtggtgatag ctacaagtcc ggagagagcg atggccacca
     1261 tggaggagtg cgtggcgctg aacattggac atgtgtggat gcaccgtgcg tttggagatg
     1321 gaagcgtttc gcccgaggca gcggcctggg ggcgggacaa cgggatccag gtgatcgacg
     1381 gcgggtgccc gcttatgttc gatcctgtgg cggacggtgg tcacaaagcg atgagggtcg
     1441 tgttcaccct caccggcaag gtccctcgaa aggtctgagg tgacgagcga tcccggcacg
     1501 gccgcaacca tcgaattggg gacctctccg cccctccaca ccggctcttc atggtgccgt
     1561 tcctccgtac accggtcctg tagcggatca tgggcaaacg ggtggcgctg atagccgtcg
     1621 accacggcgg cagtatcagc caaacggatc caaaggacct gcttggacgc ttcgccgtca
     1681 tccagatcga actggacgac gagtgaccga ggtgacggtg cgggttccct tggaggagaa
     1741 catggatctc cttcgaaaca cggcgcgggc ctgtctcggt ttcacgtcac ccgaagggcc
     1801 ccggatcgaa ccggcagcat tacggtaccg gagggatcga tatctggttg gcctagatga
     1861 gggcgcgtcg gtccccgacg aaggtgccga ggtggtcctg gtcgtggacg agggacgatt
     1921 gttcttcgaa ttgcgggccg tatacgtacg cggtcacgcg acctacgccc gcgagaggtc
     1981 cgaaggtgaa aggacctgga tcgaggtcgt cccgaccaag atcacgagct gggattacgg
     2041 acgaatgcgc ttggatcatg gtcccgactg accccgacgt ccaggagatc ctgaagggat
     2101 gcatggttgc ccgtctcgtc accatgtcga gtagcggcag acctcatgtg aacccgctct
     2161 acttcgtgtg gcacgacggc aagatccatc tcggtacgac cacgaggacg ctggcagctc
     2221 ggaatgtcgt tgcgcatccg atggtgcagc ttctcctgga cgtcgagtca gagccatccc
     2281 gagggcggat ggtgaggatc aggggcgagg ccgtgctact gactgatccg gagactctgc
     2341 gtgagtacac gcgtcgtgac gctctgaagt acttccggtc gtggaacagt ctgttgatga
     2401 gtctgaccca cctccgccaa ctgctcttga cgaggcggta cctctcgagt gacgacccgg
     2461 caaataccca ctccgtcatc gagatagcgc ccaccgatgt cgagctgctc accagcacca
     2521 cctgacggct ccgtcaatcc ccgggaggag ctggtgacaa catcgggacc ttttcccctt
     2581 acccagatcc gcccccggaa agtagatgtg gtgccagctg aggcaggagt gagtacccgg
     2641 tgcgtcgctt cccgggaacg aaggtgccga tgatcgtcct acttgtactg ctcttcgtcg
     2701 cctcctgcag cgccgaacct gaagtctcca gagaggagat ggttcagcac atccagacca
     2761 aggcgaccgc cttctcggac ttgagcctgt cctcggagat cctcgaagat ctggcatctc
     2821 accgcgtcgt ggtgttaggc gagacacatc gtct
//
LOCUS       DASUTA010000210         2159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_708925, whole genome shotgun sequence.
ACCESSION   DASUTA010000210 DASUTA010000000
VERSION     DASUTA010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708925"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VFZ80_04195"
     CDS             <1..756
                     /locus_tag="VFZ80_04195"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HEX6286661.1"
                     /translation="DEAVMPRILVRSGGPAVVVSRLLRTWGQSESMVGELLDDLYQGS
                     TNPSVAFLASAGEIKVRITAKADDRSSALAMIEPVAEEVKRRLAPWYFAEGDDTVLRI
                     LARLLGEKGWFIGTAESMTGGLVAAALTSSPGSSNYVRGGIVAYDPQLKSKLLGVSDI
                     ETVVDLETAVEMAEGGRKILEADVVVAVTGSAGPEPLERPPGTVIIAVATPEDTRARE
                     LRMPGDRERVMAYGTTSALHLARLAISGQWWES"
     gene            762..1307
                     /gene="thpR"
                     /locus_tag="VFZ80_04200"
     CDS             762..1307
                     /gene="thpR"
                     /locus_tag="VFZ80_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX6286662.1"
                     /translation="MDSPIVGRVFLAAPLPSEIRFALAERIADMSIPGKVGPADNWHI
                     TLRFLDTVDQVTYERFLAGMDPVVETSFGIRLDGFGAFPSSKKATVFWAGVGEGSTQL
                     AIINEQAEEAALAAGLLPEERPFHPHLTLSRIRPPVDVRDVVEEDLSLSWRCERLITY
                     RSHLGRGPARYEALDSLDLIG"
     gene            complement(1322..>2159)
                     /locus_tag="VFZ80_04205"
     CDS             complement(1322..>2159)
                     /locus_tag="VFZ80_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286663.1"
                     /translation="DSQFESKLDLAISPLMGLQLFVQAFFQSLDGIELSLALLGITAV
                     VLAGFSLVSRLPLVVPFGSPRYWSAVLVGRESQLEIRERPNRSSKVIYQLRPTTKGIE
                     SEPARRLMPRGWIPVETPRGQGWAVTGQLTEQVDAQSFMEDQRPSAMVDKLAAGLMRD
                     KDVTRLLARRGLIVDLGDGVEVIDPEMVGELTRSGLPRSPQTELRAEFESQVVGPFLE
                     AYRASSEISAESSHSSSVLLPTEILNFRYLTVGAPGVGSWLVLFEYEKGRPHIVGILR
                     DQ"
BASE COUNT          390 a          675 c          703 g          391 t
ORIGIN      
        1 gacgaggcag tgatgccgcg gatcctggta cggtccggag ggcccgcggt cgtggtcagc
       61 cggctcctgc ggacatgggg ccagagcgaa tcgatggtcg gtgagttgct cgatgaccta
      121 tatcagggat ccaccaaccc atcggtcgca ttcctcgcct cggcaggcga gatcaaagtc
      181 cgaatcacgg cgaaagccga cgatcgatcc tctgccctgg caatgatcga accagtcgcc
      241 gaggaggtta aacggcgtct cgccccctgg tatttcgccg agggcgacga caccgtcctt
      301 cgtatcctgg cccggttgct gggggagaag ggctggttca tcgggaccgc agagtcgatg
      361 accggtggcc tggtggcggc cgccctgacc tcttcacccg ggtcctccaa ctacgttcga
      421 ggtggcatcg tcgcctacga cccacaattg aagtcgaagc tcctcggggt cagcgacatc
      481 gagacggtgg tcgatctgga gacggcggtg gagatggcgg aagggggtcg caagatcctc
      541 gaagccgacg tggtggtcgc cgtcacggga tcggccggcc ccgagccact ggagaggccc
      601 ccgggcacgg tgatcatcgc agtggccaca cccgaggaca cgagagcgag ggagctgcgc
      661 atgccgggtg accgggagcg ggtgatggcc tacggcacga ccagcgccct ccatctcgcc
      721 cgtcttgcga tctccgggca gtggtgggag agctgacggc cgtggacagc ccgatcgttg
      781 gccgggtctt tctcgccgcc cctttgccct ctgagatccg attcgccctg gctgagcgaa
      841 tcgccgacat gtccatccca ggaaaggtgg gccctgcaga caactggcac atcaccttgc
      901 gtttcctgga cacggtcgat caggtgactt acgagagatt tctcgccggc atggatcctg
      961 tggtggagac ctcattcggc atccgcctcg acggtttcgg cgcattcccc agctcgaaga
     1021 aggcgaccgt cttctgggcc ggagtgggag agggctcgac tcagctggcg atcatcaacg
     1081 agcaagccga ggaggcagcc ttggctgcag gtctgctccc cgaggagagg ccatttcatc
     1141 cgcatctgac tctgtcacgg atacgcccac ccgtcgacgt tcgcgatgtc gtggaggagg
     1201 atctgtcgct ctcctggcgg tgcgagcggc tgatcaccta ccgaagccat ctgggacgag
     1261 gcccggcgcg ctacgaggcc ctcgattcac ttgatctcat cggctgatca cctggtcgct
     1321 gtcactggtc gcgaaggatg ccgacgatgt gaggtctgcc cttctcatat tcgaagagca
     1381 ctagccatga gcccactccc ggcgccccca ctgtcaggta cctgaagttg aggatctcgg
     1441 tgggcagcag aacgctggaa gagtgagagg actcggccga gatctcgctc gaggctcgat
     1501 atgcctcgag gaacggcccc acgacttgcg actcgaactc cgcccgaagc tcggtctgcg
     1561 gcgaccgggg cagcccactc cgggtcagct caccgaccat ttcaggatcg atgacttcga
     1621 cgccgtcgcc gagatcgacg atcaggcccc gccgggccag cagccttgtg acgtctttgt
     1681 cccgcatcaa gccagccgcc agcttgtcca ccatcgcgct cggcctctga tcctccatga
     1741 acgactgggc gtcgacttgt tcggtgagtt gaccggttac cgcccaaccc tgtcctcggg
     1801 gggtctccac agggatccag cctctcggca tcagccgtcg cgccggctcc gactcgattc
     1861 ccttggtggt gggtctgagc tgatagatga ccttgctgga gcgatttggc ctctcccgga
     1921 tctcgagctg cgactcgcgt ccgaccagaa cggccgacca atagcgtggt gagccgaagg
     1981 gcacgacgag agggagccgt gacaccaggc tgaaaccggc gaggacgact gctgtgatgc
     2041 cgagcagggc caaggagagc tcgattccgt cgagtgactg gaagaatgct tgcacgaaga
     2101 gttggagacc catcaacggc gagatggcga ggtcgagctt ggactcgaac tggctgtcg
//
LOCUS       DASUTA010000211         6032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1227700, whole genome shotgun sequence.
ACCESSION   DASUTA010000211 DASUTA010000000
VERSION     DASUTA010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227700"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="VFZ80_04210"
     CDS             complement(<1..1444)
                     /locus_tag="VFZ80_04210"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6286664.1"
                     /translation="MTIRWRHEDPGLPIAFEQCSNGEYEPEPMTPVRRQAELTARAGI
                     DDAIARTGMRRREFLRSASALAICLAAIDGSIALARGTVPGRFYPFDPLSRLDSDAAR
                     SIIGPSEFVFDVQGHLLEYDLDPSTRGDWFWGNQFPQARCEEEDDPRACFATNHFLEE
                     IFIRSDTTMTALSGLPINPEGSPLSNEVMEETRQLVQALSGDERVVVNALALPQTAPI
                     GSVLDEMTRTVEENRIAGWKTFTHFPASIHWWLDDHDPGLAQVGDRFLEHLVTIDTPI
                     LFVHKGLSNQARYGSPVDIGPAARAHPDVSFVVYHSGFEVDRREGAYTEDTSDIGVNR
                     LITSVRGAGIGPGGNVYAELGSTWWHLLRRPDEAAHVIGKLLVAFGEDRILWGTDSIF
                     YGSPQGQIEAFRAFQIPVDMQERFGYPPLTRAAKAKILGWNAAALYGIEPITEPVGFT
                     RADLELARAEHPVPSRTWGPRTVEEVREFRA"
     gene            1539..2738
                     /locus_tag="VFZ80_04215"
     CDS             1539..2738
                     /locus_tag="VFZ80_04215"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6286665.1"
                     /translation="MSRRPPLTFIFTVTLTGILSNTLVTPAIPDILDEFGLTDASAGL
                     LVAVGSLAGIVMAPTAGFLADRFGRRIVLTITLTIFGVFGGLAALAPSFEVLLLARFL
                     QGVGSAALVNLAVVLIGDNWTGLDRTRLIGRNSAILTIGLAALPLLSGALTEATSWRV
                     TFGVYTLALGTAAAAWVVLDGRRPADPPHVRDQFGEALVVLRRPVLRASILAGFLVFI
                     AIFGLFLTVLPVHLADVFGLEAGGRGLLISLPAITATIASFNLARVRLATSARTVVVF
                     SSLLLILAYVTIGLTTTLAVVVVAALTYGASEGFFIPMLQDLNMEDAPDEHRAVVIAV
                     WVGFARLGQTIGPLLAGWSVGLIGTGPTFVAGSGIAILILILGWLGPFPGERARREEP
                     VGHVPSG"
     gene            2824..4449
                     /locus_tag="VFZ80_04220"
     CDS             2824..4449
                     /locus_tag="VFZ80_04220"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX6286666.1"
                     /translation="MSEFDRHNHGESETALDAGDRAAFAAAYDRYGDRIYSFCLNMLR
                     NPEEAAEATYDVFVSAAREPDRLADPDGLQPWLFELARRETRAEGRGRAPKTGELDEA
                     EDLNDLRLLVWQAVDGLGERDVELMLLHLVEGLNGEGLASATGVDIAHLDGLVSRMSK
                     RVEKAVAPLLIARLRSDECDELGYLLGDWQGRFDSGVRARVTRHVAGCEVCQAGRATL
                     MEPASALAGIMVGPAPSALRQRVLDVVSEPAGPPPTMAPTRARRVRELSDMTMLGIFA
                     AVTLILGLIGLAVSAQFEPLEPPDPTTPPLAEDGTTTSSPGPTTTTIAIGTTTPDSNT
                     TQAPGPGEIEVSSESIDFGDDATTAEIDFTNTGGQAISFTVEPSVEAIVLSAGGEEIG
                     PGQSVTYDVLLDRETVAEGELDESIAVTWEGGSFEIAVTGVHLDNPILHNPQANPAQV
                     QVGGGSDCAGTSTTISVRVRDRSPLETVVAQWSPDGGSNRETAMAEVGGDIFEAEIGP
                     FTRVHSAQVRMVAIDELGNAGGTTIEVPVVACP"
     gene            4492..5676
                     /locus_tag="VFZ80_04225"
     CDS             4492..5676
                     /locus_tag="VFZ80_04225"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX6286667.1"
                     /translation="MKVVLAYSGGLDTSVILAWLREEYQADVVTYTADVGQGEEVEEA
                     RAKALDTGAVEAVVGDLKEEFVTDAVFPALRAAAVYETEYLLGTSLARPIITKGLVDT
                     ARSHGADAIAHGATGKGNDQVRFELSTAALAPELKTIAPWREWKMGGRADLVAYAEAR
                     GIPTPVTKESPYSMDANLYHISYEGGVLEDPWTPPPDGMFRLTTDPEDATDEPREAVI
                     GFQSGNPVSVDGEMLDPVTLLTRLNEMAGAHGVGRIDIVENRFVGIKSRGVYETPGGT
                     VLHAARRAIESIVLDREVLHLRDDLAPRYARIVYNGFWYSPERRTLQATMDRIQSAVT
                     GEARLTLYKGSVTVTGRRSPNSIYSEDIATFEADDVYDQSDAKGFINLNALRIRGFGD
                     SP"
     gene            5673..>6032
                     /locus_tag="VFZ80_04230"
     CDS             5673..>6032
                     /locus_tag="VFZ80_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX6286668.1"
                     /translation="MTLWGGRFGDEMSDVTRRFTEDTSDRRLLEFDIQGSMAHVEMLG
                     ATGTIDTEEADTLLRGLRQILEDAGSFEFTETDEDVHTAVERRLGELVGEVAGKLHSG
                     RSRNDQVALDLRLYLRRA"
BASE COUNT         1022 a         1930 c         1956 g         1124 t
ORIGIN      
        1 gagcgcggaa ttcacgcact tcctcgactg tgcgtgggcc ccacgttcga ctcgggaccg
       61 gatgctcggc acgggcgagc tcgaggtcgg ctcgtgtgaa ccccaccggt tcggttatcg
      121 gctcgatccc gtacagcgca gcggcgttcc agccgaggat ctttgccttg gcggccctgg
      181 tcagaggtgg gtaaccgaat ctctcctgca tgtcgacagg gatctggaag gctcggaaag
      241 cttcgatctg gccctgaggt gagccgtaga agatcgagtc ggtgccccac aggattctgt
      301 cttctccaaa ggcgaccagg agtttcccga ttacatgtgc cgcctcgtca ggacgtctca
      361 ggagatgcca ccaggtcgag cccagctcgg catagacatt gccacccggt ccgatgccgg
      421 ctccgcgaac agatgtgatc aggcgattca cgccgatgtc tgacgtgtcc tcggtgtaag
      481 cgccttcgcg gcggtcgacc tcgaatccgc tgtggtagac gacgaaggag acgtcggggt
      541 gggcacgggc cgcgggcccg atgtccacag gtgatccgta tcgcgcctgg ttggagagcc
      601 ccttgtgaac gaacaagatc ggtgtgtcga tcgtcactag atgctcgagg aatcggtcac
      661 cgacctgggc caggccagga tcgtggtcgt cgagccacca atggatgctc gccgggaaat
      721 gcgtgaaggt cttccagccg gcgatccggt tctcctcgac agtcctcgtc atctcgtcga
      781 gcaccgaccc gatgggagca gtctggggaa gagccagcgc attgacgacc accctctcgt
      841 ctcctgacag tgcctgcacc aactgcctgg tctcctccat gacctcattg gacagtgggc
      901 tgccttcggg gttgattggc aagcccgaca aggcggtcat cgtcgtgtcg gatcggatga
      961 agatctcctc gaggaagtgg ttggtggcga agcacgcacg cggatcgtct tcctcctcgc
     1021 aacgtgcctg ggggaactga ttgccccaaa accagtcgcc ccgagtcgag gggtcgaggt
     1081 cgtattcgag gaggtggcct tggacgtcga agacaaactc cgaggggccg atgatcgacc
     1141 tggccgcatc ggagtccagt ctggagagcg ggtcgaatgg gtagaacctt cccggaaccg
     1201 ttccccgggc gagggcgatc gaaccatcga tggcggcgag gcagatggcg agggcgctcg
     1261 ccgatcgcag aaactcacgc cgacgcattc cggtcctggc tatcgcatca tcgattccgg
     1321 cccgagcggt cagctccgct tgtcgtcgaa cgggggtcat gggctcaggc tcgtactccc
     1381 cgttggagca ctgctcgaaa gcgatcggca gccccggatc ctcgtggcgc cacctgatgg
     1441 tcatcgcaac ctcaatactc gcgcatccca cgaatcaggg gtccaagcgt tccttgaccg
     1501 tttcggcgta atcatcgacg gtccttacgc tgccccgaat gtcccgacgt cccccgctga
     1561 cgttcatctt caccgtcacg ctcaccggga tactcagtaa caccctggtc accccggcca
     1621 tcccggacat actcgacgaa ttcgggctaa ctgacgcctc tgccggactt ctcgtcgcag
     1681 tcgggtctct cgccggcatc gtcatggcgc ccacggccgg gttcctggcg gaccggttcg
     1741 gtcgccgcat cgtcctcacc atcacgttga ccatctttgg cgtgtttgga ggcctggctg
     1801 ctctggctcc gagcttcgaa gttctcttgc tggcccgatt cctccagggg gttggttctg
     1861 cggcgctcgt caatctggct gtcgtcctga taggcgacaa ctggaccggt ctcgatcgca
     1921 cacggctcat cggccgcaac tcggcgatcc tcaccatcgg attggcggct cttcccctcc
     1981 tttcgggcgc gctcaccgag gcaaccagct ggcgggtcac cttcggcgtc tacaccctgg
     2041 ctctgggcac ggctgccgcc gcctgggtcg tcctcgacgg gagacggccc gccgatccgc
     2101 cccatgtgag agatcagttc ggtgaggcgt tggtcgttct tcgcagaccg gtactccggg
     2161 catcgatcct ggccggcttt ctcgtcttca tagccatctt tgggttgttc ctgaccgtgc
     2221 tgcccgtcca cctggccgac gtatttggtc tggaggccgg ggggcgaggc ctgctcatct
     2281 ctctcccggc gatcacggca accatcgcct cgttcaacct cgcccgagtt cgtttggcca
     2341 ccagcgcccg gacggtcgtg gtgttctcct ccttgctcct gatcctcgcg tacgtgacca
     2401 tcggtctcac cacgacgctc gccgtcgtcg tggtagccgc gctgacctat ggcgcaagcg
     2461 agggcttctt catcccgatg ctccaggacc tcaacatgga ggacgcgccc gatgagcatc
     2521 gagccgtcgt catcgcggtg tgggttgggt tcgcccgtct cgggcagaca atcggcccgc
     2581 tcctcgcagg ctggtcggtt gggctcatcg gaaccgggcc cactttcgtg gccgggtcgg
     2641 gaatagccat cctcatcctg attctcgggt ggctcgggcc gtttccgggg gaacgtgcga
     2701 gacgagagga gccggtcggt cacgtaccgt ctggatgaaa tcgctgaaag ttgtttcagc
     2761 cgccgaaatt gcggttattg gtggccccgg gtgtaaggat tcaccagggc aactgcctcc
     2821 tctatgtccg aattcgatcg acacaaccac ggagaatcgg aaaccgcgtt ggacgcgggg
     2881 gatcgagctg cgttcgccgc cgcctacgac aggtatgggg atcggatcta ttcgttctgt
     2941 ctcaacatgt tgcgcaatcc cgaggaggcg gccgaggcca cttacgacgt gtttgtcagc
     3001 gcggcccggg agcccgatcg attggccgat cccgatggac tccaaccctg gttgttcgag
     3061 ctggcgcgtc gtgagaccag ggccgaaggg cgtgggcgcg ccccgaagac gggggagctc
     3121 gacgaggcgg aggatctgaa cgatctccgt cttctcgtct ggcaggcggt ggacgggctc
     3181 ggcgagcgtg acgtggagct gatgttgctt catctcgtag agggcctcaa cggagagggc
     3241 ctggcgagcg caacgggagt cgacatcgcg cacctcgacg gccttgtctc ccgcatgagc
     3301 aaacgtgtcg agaaggcggt cgcgcccctg ctcattgccc gtctgcggag tgacgagtgc
     3361 gacgagctgg ggtatttact cggcgactgg caaggccgat tcgactcggg agtgagagcc
     3421 cgggtcaccc ggcatgtggc tgggtgcgag gtgtgtcagg cgggtcgcgc cacgctgatg
     3481 gagccagcat cggccctggc agggatcatg gttgggcccg caccctcggc gctacgacag
     3541 cgcgttctcg acgttgtctc agagccagcc ggcccaccgc ccaccatggc ccccacacgt
     3601 gcgaggaggg tgcgggagct gagtgatatg acgatgctgg ggatcttcgc tgccgtgaca
     3661 ttgatcctgg ggctgatcgg cctggctgtc tcggctcaat tcgagccact cgagcctccg
     3721 gatccgacaa ctccgcctct ggcggaggac ggcaccacga cctcgagtcc gggccctacc
     3781 acgaccacca tcgccatcgg aaccaccact cccgattcca acacgaccca ggcccccggc
     3841 cctggtgaga tcgaggtgtc gagtgagtcg atcgacttcg gcgacgatgc caccacggct
     3901 gagatcgact tcaccaacac cggcggtcag gccatttcct tcaccgtcga gccgagcgtg
     3961 gaggccatcg tcttgtcggc gggtggtgag gagatcggcc cgggtcagtc cgtcacctat
     4021 gacgtcttgc tcgatcgcga gacggtggcg gagggtgagc tcgacgagtc gatcgcagtg
     4081 acgtgggagg gcggctcgtt cgagattgcc gtcacaggcg tccacctgga caatccgatc
     4141 ctccacaacc cgcaggccaa cccggctcaa gtgcaggtcg gcggagggtc tgattgtgca
     4201 ggcacctcga cgaccatctc tgtgcgagtc cgcgacaggt ctcccttgga gacggttgtc
     4261 gcacagtgga gcccggatgg gggatccaac cgcgagaccg ccatggccga ggtcggtggc
     4321 gacatcttcg aggccgagat cggcccgttc accagggttc actcggctca ggtgcgaatg
     4381 gtggcgatcg acgagctggg caacgccggc ggaaccacca tcgaagtgcc tgtggtggcc
     4441 tgcccctgac cgatctttac aagtgacagc cgacgcgcca tcgttcctgg gatgaaagtg
     4501 gtactcgcct actcgggtgg cctggacacc tcggtgatcc tcgcctggct gcgagaggag
     4561 taccaggccg acgtcgtcac ctacaccgcc gacgtcggtc agggcgagga ggtcgaggag
     4621 gcgagggcaa aggccctcga caccggggcg gtcgaggccg tcgtcggcga cctcaaggag
     4681 gagttcgtca ctgacgccgt cttccccgcc ctacgtgccg ccgccgtcta cgagaccgaa
     4741 tatctcctgg ggacctcgct ggcccgaccc ataatcacca agggcctggt ggatacggcc
     4801 cgcagccacg gcgcagatgc catcgcccat ggcgccacgg ggaaaggcaa cgatcaagtg
     4861 aggttcgagc tctcgacggc tgcgctcgct cccgagctga agaccatcgc cccatggcgg
     4921 gagtggaaga tgggcggccg cgccgatctc gtggcttacg ccgaagcgcg gggaatcccg
     4981 acaccggtga ccaaggagtc tccctactcc atggacgcca acctctatca catctcctac
     5041 gaggggggtg tgctggagga cccttggacg ccacctccgg atgggatgtt ccggttgacc
     5101 accgatcccg aggatgcaac ggacgagccg agagaggcgg tcatcgggtt tcagtcgggc
     5161 aacccggtga gtgtcgacgg cgagatgctc gatccggtga cgctgctgac ccgtctcaac
     5221 gagatggccg gggcccatgg cgtgggccgg atcgacatcg ttgagaatcg tttcgtgggc
     5281 atcaagagtc gcggcgtgta cgagacgccc ggcggcaccg tcctccatgc ggcgaggcgc
     5341 gccatcgagt cgatcgtgtt ggatcgggag gtcctccatc tccgtgacga cctcgcccct
     5401 cgctacgccc gcatcgtcta caacggcttc tggtactcac cggagaggcg caccctgcag
     5461 gcgacgatgg accggatcca atcggcggtc accggcgagg cacgattgac tctctacaag
     5521 gggtcggtca cggtcaccgg ccgccggtcg ccgaacagca tctacagcga ggacatcgcc
     5581 actttcgagg ccgacgacgt gtacgaccag tccgacgcca agggtttcat caacctgaat
     5641 gccctgcgca tccggggctt cggcgactct ccatgacgct ctggggcggg cgcttcggcg
     5701 acgagatgtc cgacgtcacc cgtcgcttca ccgaggacac ctcggaccgt cgcctcctcg
     5761 agttcgacat ccaggggtcg atggcacatg tcgagatgct cggcgcgacg gggaccatcg
     5821 acaccgagga ggcggacact ctgctgcgcg gcctccgcca gatcctcgag gatgcgggct
     5881 ccttcgagtt caccgagacc gacgaagacg tccacaccgc cgtggagcga cggctcggtg
     5941 agttggtggg ggaggtggcc gggaagcttc acagcggacg atcgcgcaac gaccaggtcg
     6001 ctctcgatct ccgtctctac ttgcgtcgtg cg
//
LOCUS       DASUTA010000212         3438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_580645, whole genome shotgun sequence.
ACCESSION   DASUTA010000212 DASUTA010000000
VERSION     DASUTA010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580645"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VFZ80_04235"
     CDS             complement(<1..751)
                     /locus_tag="VFZ80_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6286669.1"
                     /translation="MAELEFHELRLTEVTPLTDDAVAVTFDVPPELADRFDYLPGQHV
                     TVRRIIEGDDVRRSYSICAPAGSGKLRVGIKQLPGGAFSSFACSSLRAGDTLEVMSPV
                     GEFTIDIDPDRPRKAVAIAAGSGITPVLSLIATSLEQEPHSTWTLIYGNKTANSVMFL
                     DELEGLKDRYHPRFQLFHILSREGSDVPLLSGRIDDEKIRTIHDHLLGTDPVDGWYIC
                     GPYDMVMEARETLSDLGVEDSAIHDELFFAGP"
     gene            complement(751..1206)
                     /gene="paaD"
                     /locus_tag="VFZ80_04240"
     CDS             complement(751..1206)
                     /gene="paaD"
                     /locus_tag="VFZ80_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEX6286670.1"
                     /translation="MVKLAVDPRQVVASVVDPEIPVLTIEDLGILRSVEEVGDTIVVT
                     ITPTYSGCPAMRQIEDDIASALRRVGVEDFEIRVTHNPAWTTDWMSEEGHRKLADFGI
                     APPTNVSEVVCPRCRADHPRMVARFGSTACKAFMVCSSCGEPFDYFKEF"
     gene            complement(1200..1916)
                     /gene="paaC"
                     /locus_tag="VFZ80_04245"
     CDS             complement(1200..1916)
                     /gene="paaC"
                     /locus_tag="VFZ80_04245"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018738539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEX6286671.1"
                     /translation="MVPGTPEFIRLLNHADDNMVLAQRLGEWISNAPDLELDIAMGNL
                     ALDHLGVARSLYTHAGQVEGRGRTEDDLAMKRPERQFTNLLLIEQPNGDFAHTIARQT
                     FFDLYQQLLWADLAGDDDETVAGIAAKAAKENAYHLEFSGNWLIRLGDGTDESHRRTQ
                     VAVDALWMFTGEMFQGETSHYRGPWDMAVGALLEEATLSLPEDPYQRSGGRIGFHTEH
                     LGFLLAEMQWMQRSYPEMTW"
     gene            complement(1955..2218)
                     /gene="paaB"
                     /locus_tag="VFZ80_04250"
     CDS             complement(1955..2218)
                     /gene="paaB"
                     /locus_tag="VFZ80_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HEX6286672.1"
                     /translation="MWEVFVRARRGISHVHIGSLHAPDARTALENARDVYTRRGEGVS
                     VWVVPSSEISAFEPEAAAEYVEANEKIYRLATTYDIPDEVGQM"
     gene            complement(2296..3225)
                     /gene="paaA"
                     /locus_tag="VFZ80_04255"
     CDS             complement(2296..3225)
                     /gene="paaA"
                     /locus_tag="VFZ80_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEX6286673.1"
                     /translation="MGTPEERFQAIIDAESKIEPSDWMPEGYRKTLIRQIAQHAHSEI
                     IGMQPEGNWISRAPTLHRKAILMAKVQDEAGHGLYLYSAAETLGVKRNDLQELLHQGR
                     HKYSSIFNYPTLSWADMGAIGWLVDGAAITNQVMLTRCSYGPYARAMVRICKEESFHQ
                     RQGYEILIRLCNGTREQKAMAQDALDRWWWPSLMMFGPNDSDSTHSEQSMEWKIKRVS
                     NDDLRQQFVDMTVPQAEFLGLEIPDPELRWNPERGHYDFGDIDWEEFWRVVKGNGPMN
                     RERIEHKTRAWEEGAWVREAATAHAAKRAKRAA"
BASE COUNT          599 a         1051 c         1099 g          689 t
ORIGIN      
        1 tcgggccggc gaagaagagc tcgtcgtgaa tcgccgagtc ctccactccg aggtcgctca
       61 gcgtctctcg ggcttccatc accatgtcgt aggggccgca gatgtaccag ccatcgaccg
      121 gatcggtgcc gaggaggtgg tcgtggatgg tgcggatctt ctcgtcatcg atgcggcccg
      181 acagcaaggg cacgtctgag ccctcgcggc tcaggatgtg gaagagctgg aacctggggt
      241 gatagcggtc cttcaggcct tcgagctcgt cgaggaacat caccgagttg gcggtcttgt
      301 tcccgtagat cagggtccag gttgagtgtg gctcttgctc gagcgaggtg gcgatcagag
      361 agaggaccgg ggtgatcccc gagcctgcgg cgatagcaac ggccttacgg ggacgatccg
      421 ggtcgatgtc gatggtgaac tcgcccaccg gggacatgac ctcgagagtg tcgccggcgc
      481 ggagactgga acatgcgaaa ctgctgaaag cccctccggg aagctgtttg atcccgacgc
      541 ggagcttgcc cgatcctgcg ggggcacaga tcgaatagga cctgcgcacg tcgtcgccct
      601 cgatgatgcg gcggacggtg acgtgctgac ctggcaggta gtcgaaacga tcggcgagct
      661 ctggcggcac gtcgaaggtg acggcgactg catcgtcggt caaaggggtg acttcggtca
      721 gccgcagctc gtggaactcg agctcagcca tcagaactct ttgaagtagt cgaacggctc
      781 gccgcacgac gagcagacca tgaatgcctt gcacgccgtg gaaccgaatc tcgccaccat
      841 ccgcggatgg tccgcccgac atcgagggca aacgacttcg ctgacattgg taggaggggc
      901 gatgccgaaa tcggcgagtt tgcgatgacc ctcttctgac atccagtcgg tggtccaggc
      961 cggattgtga gtgacccgga tctcgaagtc ctcgacccca actcgacgca gcgccgaagc
     1021 gatgtcgtct tcgatctggc gcatcgccgg acagccggag taggtcggcg tgatcgtcac
     1081 cacgatcgtg tctccgacct cttcgaccga gcggaggatg cccaagtcct cgatggtaag
     1141 gaccgggatc tcggggtcga cgaccgatgc cacgacctgg cggggatcga ccgcgagttt
     1201 caccatgtca tctccggata gctgcgttgc atccactgca tctcggccag gaggaacccg
     1261 aggtgctcgg tgtggaaccc gatcctgccc cccgacctct gatacggatc ctcggggagg
     1321 gacagagtcg cctcctcgag caaggcgccg acggccatgt cccatgggcc gcgatagtga
     1381 gacgtttcgc cctggaacat ctctccggtg aacatccaca gcgcgtcgac ggcgacctgg
     1441 gtgcgccgat gcgactcgtc ggtcccgtca ccaaggcgta tcagccagtt gcccgagaac
     1501 tccaggtgat aggcgttctc cttggcggcc ttggcagcga ttccggcgac ggtctcgtcg
     1561 tcatcaccgg cgaggtcggc ccagagcaac tgctgataga gatcgaagaa tgtctggcgg
     1621 gcgatcgtgt gagcgaagtc gccgttgggc tgctcgatga gcaggagatt ggtgaattgc
     1681 cgctcggggc gcttcatggc gaggtcgtct tcggttcgtc ctcgaccctc gacctggccg
     1741 gcgtgtgtat agagagaacg agccaccccg agatggtcga gggcgagatt acccattgcg
     1801 atgtccagct cgaggtcggg cgcattcgat atccactccc cgagtctttg agccagaacc
     1861 atgttgtcgt cggcatggtt gagcaaacgg atgaactcgg gcgtgcctgg caccaccggg
     1921 gttccctgcg gtgccccact gactgtctgc tcggtcacat ctgccccact tcgtcgggga
     1981 tgtcataggt ggtggccagg cgatagatct tctcattggc ctcgacgtat tcggccgcgg
     2041 cttcaggctc gaacgccgag atctcgctgc tcgggaccac ccagacgctg acaccttcgc
     2101 cgcgacgggt gtatacgtcc cgggcgttct cgagagcggt tcgggcgtct ggggcatgca
     2161 ggcttccgat gtgaacgtgg gaaatgccac gacgagccct cacgaatact tcccacatgg
     2221 gccagccttc ggctgccccc tctatcgtct cgtccagctc ggccctctca cccggagcga
     2281 cggggacgtc gtaggtcatg ccgctctctt cgcccgcttg gcggcgtgag cggtcgctgc
     2341 ctcacgaacc caggcgccct cctcccaggc acgggtcttg tgctcgatgc gctcacggtt
     2401 catggggccg ttgcccttga cgacacgcca gaactcctcc cagtcgatgt ctccaaagtc
     2461 gtagtgaccg cgttcggggt tccagcgaag ctcggggtca gggatctcga gaccgagaaa
     2521 ctcggcttgc gggactgtca tgtcgacgaa ttgctgacgc aggtcatcgt tggagacacg
     2581 cttgatcttc cactccatcg actgctctga gtgggtggag tcgctgtcgt tgggcccgaa
     2641 catcatcagc gaaggccacc accacctatc gagtgcgtcc tgggccatcg ccttctgctc
     2701 tcgggtgccg ttgcacagcc tgatcagaat ctcgtagccc tgacgctggt gaaacgactc
     2761 ctccttgcat atccggacca tggcacgggc atatggccca taagaacacc gggtcagcat
     2821 cacctggttg gtgatggcgg cgccatcgac cagccatcca atggcaccca tgtcggccca
     2881 ggagagggtc gggtagttga agatcgacga gtacttgtgc ctgccctgat ggagcagctc
     2941 ctggagatcg ttgcgcttga caccgagtgt ctcggctgcg gaatagaggt agaggccatg
     3001 tcctgcttcg tcctggactt tcgccatgag gatcgccttg cgatgcagtg tgggcgcccg
     3061 gctgatccag ttgccctcgg gttgcatgcc gatgatctcg gagtgggcgt gctgggcgat
     3121 ctggcggatg agcgtcttcc gataaccctc aggcatccag tcactcggct cgatcttgga
     3181 ctcggcgtcg atgatcgcct gaaacctctc ctctggtgtt cccatgtcgg cagtgtatgc
     3241 gaacgaacgt tcgtttgtcc accctccagg ggaaccacct cgacctacgg tgacgtcaca
     3301 attcccgatg gatggagatg tgaggacttc gttcggtcgt ctcagcgcgc tggccgtggc
     3361 cggagccgtg ctcgcctctg ccatcgcggt gacgctgccc ctgcctgctc agggatgctc
     3421 gtgcgctgct ccttccga
//
LOCUS       DASUTA010000213         4018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_709782, whole genome shotgun sequence.
ACCESSION   DASUTA010000213 DASUTA010000000
VERSION     DASUTA010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709782"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..770)
                     /locus_tag="VFZ80_04260"
     CDS             complement(63..770)
                     /locus_tag="VFZ80_04260"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6286674.1"
                     /translation="MPPEPSAVLLDVGGVFLLPSHAHIRSALSRIDHVVTDDSAIDRA
                     HYVSVRVFPMDLDPGEYMSPYWTEYLNTYSRELKVDDSLIDEAIEHLRNEYVTGGLWS
                     HVVEGSPEGLRKLVDTGVPVGIVSNSDGTVEARLEEMGVLQVGPGPGVEVRCLVDSGH
                     VGVEKPDPKIFDYAFEVLDVPRENVWYVGDTPAYDIVGAQRAGLRPILMDPFGVNGDF
                     GVTCVKSLSDVAALVSG"
     gene            938..1177
                     /locus_tag="VFZ80_04265"
     CDS             938..1177
                     /locus_tag="VFZ80_04265"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX6286675.1"
                     /translation="MKQDTTSQQVHSALNDGWMKAGSFASSIIAGTLLGYFADRWLGT
                     DPWLVIAGILLGSYAGFVKMWQYLREMDDKPARDS"
     gene            1167..1583
                     /locus_tag="VFZ80_04270"
     CDS             1167..1583
                     /locus_tag="VFZ80_04270"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEX6286676.1"
                     /translation="MTADNDSVAVESILARHTVARAVYVAPVLILVFWITRGWDGAWS
                     AALGVGIVVVNFLLAGAMLSISAKISLAVYHAAALFGFFLRLGLMVLTVLLIAQLLPI
                     DRLAFGISAVVAYLVLLSWEAVAVSKGRERNLDWTP"
     gene            1580..2380
                     /gene="atpB"
                     /locus_tag="VFZ80_04275"
     CDS             1580..2380
                     /gene="atpB"
                     /locus_tag="VFZ80_04275"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX6286677.1"
                     /translation="MILAAEECDLTQPVCAPENVSELFVFDTTLWNTPITRTIFLFFL
                     AALIVIGMLYLAYRKPNLIRSKFQTAIDAVTGFVRDEVSIGIIGPEGLKYYPYLLSIF
                     MFILVGNLFEVTPLINFPITSRMAIPGFLAIVTYLIFVVVGFAKNGFTYLGDIVWPRS
                     VPVAMRPLVGLIEFVSYFAIRPFSLAVRLLANMVAGHTMLTLLLVTGWVFMTAFGDVS
                     VAKQGAGVLWFTLGLGIYVFEILVAVLQAYIFTLLSAVYIQTSVHPEH"
     gene            2437..2700
                     /gene="atpE"
                     /locus_tag="VFZ80_04280"
     CDS             2437..2700
                     /gene="atpE"
                     /locus_tag="VFZ80_04280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016469811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX6286678.1"
                     /translation="MDAVLAVVNAGVVLAQEAGGITGSLNVVGYGLAAIGPGIGVGIV
                     AGNAVQAMARQPEVAGMLRTTMFLGIAFTEALALIGFVLFFLG"
     gene            2700..3251
                     /locus_tag="VFZ80_04285"
     CDS             2700..3251
                     /locus_tag="VFZ80_04285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX6286679.1"
                     /translation="MIFAQTFLIAAEESEGGSDGLSILLPPLNELIAGIIAFAIVFFF
                     VWRFAVPSINRMLEQRQAAISGQIADAEKAKTEAESLLADYRSQMNQAKAEGNKIIDE
                     ARQQGEQVKADIIAKAEAEAEQIRSKAREEAAGEKARALAEAKSQVGDISVDLAGKIV
                     GESLDEQAHRALIDRYLADLEKL"
     gene            3263..3799
                     /gene="atpH"
                     /locus_tag="VFZ80_04290"
     CDS             3263..3799
                     /gene="atpH"
                     /locus_tag="VFZ80_04290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX6286680.1"
                     /translation="MADDRINGYARGIFEMASGEGSLDRVESEMLTISRAFETSPELR
                     SNLTDPQLPLERKQAIVDELLGGRASSLTVGVVQLLVSQNRASELPAIASAVAGAAAA
                     SRDRAVAEVRSAVPLDEATVQRLTASLSRATGKDVEVKVIVDPSVIGGIVARVGDTVI
                     DGSIARRFESVRQAVRSN"
     gene            3805..>4018
                     /locus_tag="VFZ80_04295"
     CDS             3805..>4018
                     /locus_tag="VFZ80_04295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019339.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX6286681.1"
                     /translation="MSDLTITADQITESLRSQLSGWQPDTSKETVGYVTSIADGVARV
                     EGLPSVMASELLEFPEGLVGVALNIDE"
BASE COUNT          748 a         1279 c         1186 g          805 t
ORIGIN      
        1 cgccctaccc ccacgacttc gccgggcacg tctagagaga gagatatcgc cacgatcgct
       61 gatcagccgc ttacgagcgc tgccacgtca gacaacgact tcacacaggt caccccgaag
      121 tcgccattga ccccgaaagg atccatcagg atcggccgga gaccggcacg ctgggcacca
      181 acgatgtcgt aagcaggggt atcgccgacg taccagacgt tctcacgtgg cacgtcgagc
      241 acctcgaatg catagtcgaa gatcttcggg tccggtttct cgacaccgac gtggcccgag
      301 tcgacgaggc agcgaacctc gactcctggg cccggtccaa cctggagcac acccatctcc
      361 tcgagcctcg cctcgaccgt cccatccgag ttggagacga tccccaccgg cacgccggtg
      421 tccaccagct tgcggagccc ctcgggcgac ccttcgacga catggctcca cagccctccg
      481 gtcacgtact cgttgcggag atgctcgatc gcctcatcga tcaatgagtc gtcgactttc
      541 aactcccggg agtacgtatt caggtactcg gtccagtagg ggctcatgta ttcgccagga
      601 tcgaggtcca tcgggaagac ccgcaccgac acgtagtggg cgcggtcgat cgctgaatca
      661 tctgtcacca cgtggtcgat gcggctgaga gcggatcgaa tgtgggcatg ggatgggaga
      721 aggaacactc ccccaacgtc gaggaggacg gctgatggct caggtggcat ggggttccac
      781 gctaaccgga gtgcaatgaa cacccctacg gcgtgttcat tgcacgccgg acgatgagcc
      841 tgtcgtgttt gctaggttcg atttggccta ggcaactcct atcaataatc tcttgtgaag
      901 aaattcacaa gccccctagg cgtgaggtca ggcccctgtg aagcaagaca ccacctccca
      961 acaggtccac tcggcactca acgacggctg gatgaaggcc ggctcgttcg ccagttcgat
     1021 catcgccggc accctcctcg gctacttcgc cgatcgatgg ttgggaaccg atccatggtt
     1081 ggtgatcgcc gggatcctgc ttggctctta tgccgggttc gtgaagatgt ggcaatacct
     1141 gagagagatg gatgacaaac cagcccgtga cagctgacaa cgactcagtc gcggttgaga
     1201 gcatcctcgc ccgccacacg gtcgcccgtg cggtctacgt ggccccggtc ctcatcctcg
     1261 tcttctggat cacccgtggc tgggatggcg cgtggagcgc cgcccttggt gtcggcatcg
     1321 tggtcgtcaa cttcctcctg gcgggagcca tgctctcgat ttcggccaag atctcactcg
     1381 ctgtttatca tgccgccgcc ctgtttggct tcttcctgag gttggggttg atggtcctca
     1441 ctgttctgtt gatcgcacag ttgctgccca tcgaccgcct cgccttcggg atctctgcag
     1501 tcgtggccta tctggtcctg ctgtcgtggg aagccgtggc ggtttccaag ggacgagaaa
     1561 ggaacctgga ttggaccccg tgatcctcgc ggccgaagaa tgcgacctga cacagccggt
     1621 gtgtgctccg gagaacgtct ctgagctctt cgtgttcgac accaccctct ggaacacacc
     1681 gatcactcgc accatattcc tcttcttctt ggctgcgctg atcgtcatcg gcatgctcta
     1741 cctcgcctac cgcaagccca acctcatccg ctccaagttc cagactgcga tcgacgccgt
     1801 caccggattc gtcagagatg aggtttcgat cggcatcatc ggccccgaag ggctcaagta
     1861 ctacccgtac ctgctgtcga tcttcatgtt catcctcgtg ggcaatctct tcgaggtgac
     1921 gcccctgatc aacttcccga tcacctcacg tatggcaatc ccggggttcc tggcgatagt
     1981 cacctacttg atcttcgtgg tcgtcggttt cgccaagaac ggattcacct atctgggcga
     2041 catcgtctgg ccgaggagcg tcccggtggc gatgcgcccg ctggtggggc tcatcgagtt
     2101 cgtctcctat ttcgccatcc gccccttctc cctggctgtc cggctcctcg ccaacatggt
     2161 tgccggtcac acgatgctga ccctgctcct ggtcaccggg tgggtgttca tgaccgcttt
     2221 cggtgacgtc tcggtggcca aacagggtgc cggcgtcctc tggttcaccc tgggcctggg
     2281 catctacgtc ttcgagatcc tcgtcgcggt gctccaggca tacatcttca ctcttctcag
     2341 cgccgtgtac atccagacct cggttcaccc cgagcactga caaccaagag aaagacgtct
     2401 cgtagacaac ccacgagagg gaaaggatag gaagcaatgg acgcagttct tgcagtagtg
     2461 aacgccggag tggtcctcgc acaggaggcc ggcggcatca ccgggagctt gaacgtcgtc
     2521 ggatacggtc tcgccgccat cggccccggc atcggtgttg gcatcgtcgc cgggaacgcg
     2581 gtgcaggcca tggcccgtca gcccgaggtg gccggcatgc tccggacgac gatgttcctg
     2641 ggtatagcct tcaccgaggc tctcgccctt atcggcttcg tgctcttctt cctcggctga
     2701 tgatcttcgc tcagaccttc ttgatcgcgg ccgaggaatc cgaaggaggc tccgacggtt
     2761 tgtcgatcct cctcccgcca ctgaacgagc tgattgcggg gatcatcgcc tttgcgatcg
     2821 tcttcttctt cgtctggaga tttgccgtcc cgtcgatcaa caggatgttg gagcagcgtc
     2881 aggctgcaat ctccggccag attgcagatg cggagaaggc gaagaccgag gctgagagtc
     2941 ttctcgccga ctatcgcagt cagatgaacc aggccaaggc cgagggcaac aagatcatcg
     3001 acgaggctcg ccaacagggc gagcaggtga aagcggacat catcgccaag gccgaggccg
     3061 aggcagagca gatccgctcc aaagcgcgcg aagaggctgc gggtgaaaag gcccgggctc
     3121 tggccgaggc caagagccag gtgggcgaca tatctgtgga tctcgccggc aagatcgtcg
     3181 gagagagcct cgacgagcag gcccatcgcg ccctgatcga ccgctatctc gccgacctcg
     3241 agaagctgta ggacgatcat tcatggctga cgacaggatc aacggctacg cacggggaat
     3301 cttcgaaatg gcctccgggg aaggctccct cgaccgcgtc gaatccgaga tgctcacgat
     3361 ttcgcgtgcc ttcgagacct cgcctgagct ccggtcgaat ctcaccgatc cccagttgcc
     3421 tctcgagaga aagcaggcca tcgtcgacga gctgctgggt ggtagggctt cttcgctcac
     3481 cgtgggcgtg gttcagctgc tggtcagcca gaaccgtgct tccgaactgc ccgccatcgc
     3541 cagcgcagtc gccggcgccg ccgctgcatc acgagacaga gccgttgccg aggtgcgttc
     3601 ggcagtgccc ctcgacgagg ccaccgtcca acggctgacc gcctcgctga gccgagccac
     3661 cggcaaggac gtcgaggtca aggtcatcgt cgacccatcg gtcatcgggg gcatcgtcgc
     3721 ccgggtgggc gacacagtca tcgacggttc gatcgcgaga cgtttcgagt ccgtacgcca
     3781 agccgtcagg agtaattgac cgacatgtct gatctaacaa tcaccgccga ccagataacc
     3841 gagtcgctcc ggtcgcagct ctcgggttgg cagccggaca cttccaagga aacggtcggg
     3901 tacgtcacat cgatcgccga cggtgtggcc agggtggagg gtctgcccag cgtcatggcc
     3961 tccgagctgc tcgagtttcc cgaagggctg gtgggtgtgg ccctcaacat cgacgaag
//
LOCUS       DASUTA010000214         4095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_968795, whole genome shotgun sequence.
ACCESSION   DASUTA010000214 DASUTA010000000
VERSION     DASUTA010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968795"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..958
                     /locus_tag="VFZ80_04300"
     CDS             185..958
                     /locus_tag="VFZ80_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX6286682.1"
                     /translation="MPTVADIALGIAERTNPDQTPHWDPVGLQLGDPDAVVERIAVCH
                     EVNEEVVTAVEADPVDLVVTYHPLLFSPTTRVLAGRSAGARSYRLIRANVSLLVTHTD
                     FDSAPGGTADALAAFLSLRDVQPFGGDSDSGLGEIGRFGGFQGTLATIDAQVTDRFGP
                     IGIRISGDREADIDTVAVVPGSGAAFIEMAAEVAQVLVTGDVSHHRAVAARDLGLSIV
                     DPGHTATERPGMAALVELVSALSDAEVVDLTGIDPQTWR"
     gene            1173..1880
                     /locus_tag="VFZ80_04305"
     CDS             1173..1880
                     /locus_tag="VFZ80_04305"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEX6286683.1"
                     /translation="MQLTSLTDLLDLQEIDLQIDRLLDERQSLPELALYKRTHDSVQE
                     GESRRGVAADELRQLELALDKAEGELEILENKLKEHETRLFAGGMSARETEHMRLEVQ
                     SLKGQQSALEEKVLGLLEEVDPAREKVASIQGELDDLEATKAELESAIKDQWKRIDAE
                     LARKEERKIGALAPIDPELLELYEKLRDIKEGVAVAPYDHGVCGGCHMTLSPAEQEEA
                     FASELPRCVHCRRILVA"
     gene            1893..2426
                     /locus_tag="VFZ80_04310"
     CDS             1893..2426
                     /locus_tag="VFZ80_04310"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX6286684.1"
                     /translation="MLFWHLGGAVWLFRWIFRDPKVDVRFLVLGAILPDLIDLPIGTL
                     VLADRYSTGELWFHSLIIPTLFISIVLVATRRGRRRRAWMALGVGWLFHLVLDGVWAT
                     QEVFLWPFFGDIPAGRAPYWPLAWERALNDPWRWVEEAVGLGYLVWLWFALGLGRPER
                     RSMTLQTGRLPEYVAET"
     gene            2410..3084
                     /locus_tag="VFZ80_04315"
     CDS             2410..3084
                     /locus_tag="VFZ80_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286685.1"
                     /translation="MSLRLEAALAVALLVVVSCSGDPSTDSVESLSPGVGASSPTEAV
                     TELVTAINVPDFSGASRLAMPGHAALASLAEGATFGDVAEAIREGDQAVAANFWAGFA
                     QGAGSFLGGDVNVNDAGTVSRDGVEFHQVSVATEDGGTRSVLIRESDGYRIDLFASFG
                     AGLADKMMGPVERLINTQSEDARLILANLREIVPSLLVASELPGTATDVSQQVLALVE
                     VITRIG"
     gene            complement(3086..>4095)
                     /locus_tag="VFZ80_04320"
     CDS             complement(3086..>4095)
                     /locus_tag="VFZ80_04320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6286686.1"
                     /translation="PGTRKYRVMTKFAYFCGHEQWQPEELVEHAVLAERAGFDMVVVS
                     EHFHPWVDDESASGFAFSTIGAMAAATSSIAFATGVTTPLFRYHPAVVAQAAATLDRL
                     SGGRFVLGVGTGENINEGPLGYEFPAYAERNARMTESLEIMRRLLDGEKLTYHGKYYE
                     TDRAKLYSPPLGDVPILMAAGGPKSATLAGQMAQGVITSVKDPSETMERVVEPLRAAA
                     SEAGKPTPTVLATRWSIYAADEDEAWEALHSWRGLRAPGRLEAVDPETLRKRADELPR
                     EEVLGRYSLVHSVDEIVEVYRPLVSDLGADIVTFQMASLDQPALIEMLGGEVVPRLKE
                     A"
BASE COUNT          725 a         1253 c         1333 g          784 t
ORIGIN      
        1 tcgcgtagcg gcgtcgctgc aagacggtgt ctccggggat ctcaggcatg cctccgacga
       61 ggatgcggaa aggctcacca ccggtatggg agtcgatgac ggtcagccgc cgccagtcgg
      121 agggcggttc ccagcggagg gctcgctcat ggtccatgtc tcgctgagcc tacgatgccg
      181 gacagtgcca actgtcgccg acatcgcact cgggatcgcc gagaggacga atcccgatca
      241 gaccccgcat tgggatccgg tgggtcttca actcggtgat ccggacgcgg tcgtcgagcg
      301 aatagctgtc tgtcacgagg tgaacgagga agtggtgaca gccgtggagg cggatccggt
      361 cgacctggtg gtcacctatc accctctgtt gttctctccg accacgaggg ttctggccgg
      421 gcgatcggcc ggggcgcgct cgtaccgctt gattcgggcc aacgtgagcc tcctggtgac
      481 ccacaccgac ttcgactcag caccaggagg gacggctgac gccttggccg cgttcctgag
      541 cctgagggac gttcagccgt tcggcggaga ctccgactcg ggcctcggcg aaatcggaag
      601 gttcggggga tttcagggga ccctggcgac gatcgacgcc caggtgaccg atcgcttcgg
      661 acccatcggg attcggatca gtggagacag ggaggccgat atcgacacgg ttgccgtcgt
      721 ccccggttcg ggagcagcat tcatcgaaat ggccgccgag gtggctcagg tcctcgttac
      781 cggcgatgtc tcacatcatc gagccgtggc cgcccgcgac ctcggccttt ccatcgtcga
      841 ccccggtcac accgccacag agcggccggg gatggcggcc ctcgtggagt tggtgagtgc
      901 cctgtcggat gcagaagtgg tcgacctgac aggcatcgac ccccagacat ggcggtagcg
      961 gggggtcttc tggccggtct cccatctgac gtgtgacgaa cacggtgtag gcctgtgttc
     1021 attgcacgtg ggtctatccc acccgcatcc gttcgtctga cgtgtgacga acacggtgta
     1081 ggtgtgttca ctgcacatga gtccatagga acaggctctt cgacggtgtg gcctcgttcg
     1141 tgtttcggtt gccgggctac tctgcctgat ccatgcagtt gaccagcctc actgacctcc
     1201 tcgacctcca ggagatcgat ctgcagatcg atcgtctcct cgatgagcgg cagtcgcttc
     1261 ccgagctggc cctctacaag cggacccacg acagcgtcca ggaaggcgag agcagaaggg
     1321 gcgtggccgc cgacgagctg agacaactcg agctggctct ggacaaggcc gaaggcgagc
     1381 tggagatcct cgagaacaag ctcaaggagc acgagacacg actcttcgcc ggggggatga
     1441 gtgcacggga gaccgagcac atgcggctcg aggtccagag cctcaagggg cagcagagtg
     1501 cactcgagga gaaggtcctg ggcttgttgg aggaggtcga cccggctcgg gagaaagtgg
     1561 cttcgatcca gggtgagctg gacgacctcg aggcaaccaa ggcggagctc gagtccgcga
     1621 tcaaggatca gtggaagagg atcgatgccg agctggcgag gaaggaagag cgcaagatcg
     1681 gagctctcgc tcccatcgac cccgagctgc tcgagctcta cgagaagctg cgagatatca
     1741 aagaaggggt cgcagtggcc ccctacgacc acggcgtctg tggcggttgt cacatgaccc
     1801 tctcgcctgc tgagcaggaa gaggcgttcg catcagagct gccgaggtgc gtccactgcc
     1861 ggcgcatcct cgttgcctag tgaagtgctc tgatgctctt ttggcacctc gggggagcag
     1921 tatggctctt ccgttggatc tttcgcgacc ccaaagtcga tgtccgattt ctcgtgttgg
     1981 gtgcaatcct ccctgatctg atagatcttc cgatcggcac cctcgttctc gccgatcgct
     2041 attccaccgg agagctgtgg tttcactcgt tgatcatccc gactttgttc atatcgatcg
     2101 tcctcgtcgc cactcgaagg gggcggcgac gccgcgcctg gatggccctc ggcgtgggat
     2161 ggctctttca tctcgtccta gatggcgttt gggcgacaca ggaggtgttt ctctggccat
     2221 tcttcggcga catccccgca ggccgggcgc cttactggcc gctggcttgg gagcgcgcct
     2281 tgaacgatcc gtggcgctgg gtcgaggaag ccgttggact gggatatcta gtgtggctct
     2341 ggttcgccct cgggttgggc cgaccggagc gccggtccat gaccctgcag acagggagac
     2401 tgccagagta tgtcgctgag acttgaagcg gccttggccg tcgcactcct agttgtcgtc
     2461 tcgtgctccg gtgacccatc gacagactcg gtcgaatccc tgtctcccgg agtgggggcg
     2521 tcttctccca ctgaagccgt gaccgagctg gtcactgcga tcaacgtccc cgacttctcc
     2581 ggggcctccc gactggccat gcccggtcat gcagctctcg cctccttggc cgagggagcg
     2641 acattcggag acgtcgccga ggcgattcgg gagggtgacc aggcggtggc cgccaacttc
     2701 tgggccgggt tcgcccaggg cgcgggcagc ttcctcggtg gcgacgtcaa tgtcaacgat
     2761 gccggcacgg tgagccgtga cggggtggaa ttccatcagg tgagtgtcgc gaccgaggat
     2821 ggaggaacca gatcggtgct gatcagggag tccgacggct accggatcga cctattcgcc
     2881 tcgtttggag ctggcctcgc cgacaagatg atgggaccgg tggaacgcct catcaacacc
     2941 caaagcgaag acgcacgcct catcctggcc aatctgcggg agatcgtccc ctccctcctc
     3001 gtcgcctccg agctccccgg cacagccact gatgtctctc agcaagtgct tgcgttggta
     3061 gaggtgatca cccggatcgg ctgattcacg cctctttgag cctgggcacc acttcgccac
     3121 ccaacatctc gatgagggcc ggctgatcga gcgaagccat ctgaaacgtc acgatgtcgg
     3181 cgccaaggtc tgacaccagt ggccggtaca cctcaacgat ctcatcgacc gagtgcacga
     3241 ggctgtaccg accgagaacc tcctcacggg gcaactcgtc ggcgcgcttg cgcagcgttt
     3301 caggatcaac tgcttccagt cgccccggcg ccctgagccc acgccaagaa tgcaaggcct
     3361 cccaggcctc gtcctcgtca gctgcgtata tggaccaccg ggtggcaaga accgttggcg
     3421 tcggcttgcc agcttcggat gctgcggcac gtagaggttc gacgactcgc tccatcgtct
     3481 cggatgggtc tttgaccgat gtgatcacac cctgggccat ttggcctgcg agtgtcgccg
     3541 acttgggccc tccggctgcc atcagaatcg gcacatcgcc gaggggcggc gagtagagct
     3601 ttgccctgtc ggtctcgtag tacttgccgt gataggtcaa cttctcacca tcgaggagac
     3661 ggcgcatgat ctcgagcgac tcggtcatgc gagcattccg ttccgcgtac gcggggaact
     3721 cgtagcccag cggtccctcg ttgatgttct cccctgtgcc cactccaagg acgaaacgac
     3781 cgcccgaaag acggtcgaga gtggctgccg cctgcgcgac cacggcgggg tggtacctga
     3841 agaggggagt ggtgacgccg gtggcgaatg cgatggatga ggtggcggcg gccatggcac
     3901 caatggtgga gaaggcaaaa cctgaggccg actcgtcatc cacccaggga tggaagtgct
     3961 cggagacgac gaccatgtcg aagccggccc tctcggccag gacggcgtgc tcgacgagct
     4021 cttccggttg ccattgctcg tgaccgcaga agtaggcgaa tttggtcatg acccgatatt
     4081 tgcgcgtccc gggac
//
LOCUS       DASUTA010000215         3590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1228577, whole genome shotgun sequence.
ACCESSION   DASUTA010000215 DASUTA010000000
VERSION     DASUTA010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228577"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1440
                     /locus_tag="VFZ80_04325"
     CDS             <1..1440
                     /locus_tag="VFZ80_04325"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX6286687.1"
                     /translation="FVAVSGSNVALFLGLLAVVAGPLSMGPRRRALLGLAGLPVYVAA
                     TRFEPSVIRAAVMAAIALAGRLVDVVLEAWQLLSLAVALLVVLDPPLTDNVGFQLSVA
                     ATAGVLVGARWPVEGAIRRALAMTMGAQTAVGPLLLLHFESVPLLSPIVNLLAAPLVT
                     ASTLTGALGALGVGFLIPPASWLAELVLLLARTASGWPQLDAAEFAALAVGGLVVAAL
                     PRLRIHLVVPVAVALVIAMLVPNATIRPGTVAVLDVGQGDAILMSGGDGSFALVDGGP
                     DPALIVSRLRDYGVTSLELVVLTHVHADHVTGLTGVIGALPVGAVWADTEPHTTGASA
                     DFFTHLESLGMTASAPAVGDRWQLGALHLTVEGPVRRYASPNDQSIVVTVTGSSRSML
                     LSGDIETFAQADLSHLRADVLKVPHQGAATSDPDWLAEVGADQAVISVGPNDFGHPVD
                     WVIKTLESSGASVLRTDLAGDVLVDLTTP"
     gene            1687..2064
                     /locus_tag="VFZ80_04330"
     CDS             1687..2064
                     /locus_tag="VFZ80_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX6286688.1"
                     /translation="MASRAGVDRLHALELQVLHRMIDSQDRSVIAPAASVVDSDEGRS
                     ILTRNQTIWLDAPEDVLSVRRAEGDHRREVGREDEKALERRRRPFWERIAAVRIDTSG
                     SVGEVVETVLREIRRIEAGQNRS"
     gene            2160..2975
                     /locus_tag="VFZ80_04335"
     CDS             2160..2975
                     /locus_tag="VFZ80_04335"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX6286689.1"
                     /translation="MPDALRLGSRELPLGGVTHVMGVINVSPESKNAHTVARSIDEAL
                     LMADRYRAWGAAIVDVGGQSSHLDNETIAASDEVSRVVPVIEALCAEGHIVSVDTWKS
                     DVAQAAIEAGAVIVNDTGGLAAPEMREVVADSDVAAVAVYIEAANPHAVNEVQIRDDK
                     AEHTANRFRSLLAELTKAGVANVILDPGIALNYRGDYETYSRMQLQVIAGSDHLKALG
                     RPVLIPIPRKRDGHRLAVYMGLALEHGADIIRVHDVAMACDLVDLFGRSAPLR"
     gene            2980..>3590
                     /locus_tag="VFZ80_04340"
     CDS             2980..>3590
                     /locus_tag="VFZ80_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286690.1"
                     /translation="MTLMVVAGPSSPGPGEREQMLELAAKHLVTQGVARDQVVRIDVP
                     GRGAGEDGDGSLRAELEPMIPLLQSGSLFGDAQGLELMDAQNLQKSELETLIALLDGA
                     DLSAIEVVLIYGGAVPKAVKDLGGPSVSVKKMWERQAGQWLEAEIRERGLELDKSVVQ
                     ALIQRFGSDTASLGQVLDQLAETPGKITAELVLDRFKNRPDEPT"
BASE COUNT          591 a         1099 c         1192 g          708 t
ORIGIN      
        1 ttcgtagcag tgtccggctc caacgtggca ctctttctcg gcttgctcgc agtcgtggca
       61 ggccctttga gcatgggccc gagacgcaga gctctgctgg gactggccgg tctgcccgtg
      121 tacgtggccg ccacccgctt cgagccctcg gtgatacgtg cagccgtgat ggccgcgatc
      181 gctcttgctg ggcgtctcgt ggacgtcgtc ctcgaggcct ggcagcttct gtcgctggcg
      241 gtcgcgctgt tggtcgtcct cgatccgccg ttgaccgaca acgttgggtt ccagctgtcg
      301 gtggcggcaa ccgccggagt tctggtgggg gcgcggtggc cggtcgaagg ggcgatacgc
      361 agggcgctcg caatgacgat gggagcacag accgcggttg ggccgcttct gttgctccac
      421 ttcgagtcgg ttcccctcct ctcgccgatc gtgaacctgc tggctgcccc tttggtcacg
      481 gcatcgaccc tcacgggtgc cctcggtgcg ctgggagttg ggtttctgat ccccccggcc
      541 tcctggctgg cggaactggt cctgctgctg gcgcgaactg cttcgggctg gccacagctc
      601 gacgctgccg agttcgctgc acttgccgtg ggcgggctcg tcgtcgcagc gctaccgagg
      661 ctgcgtattc acctggtcgt gccggtggcg gttgcgctcg tgattgccat gttggttccc
      721 aatgcaacca ttcggcccgg gacggtggca gtcctcgacg ttggacaggg cgatgcaatc
      781 ctcatgtccg gtggcgatgg cagcttcgcc ctggtggatg gtggaccgga cccggccctc
      841 atcgtctccc ggctgcggga ttacggagtc acatcgctcg agctcgtcgt tctgactcac
      901 gtgcacgccg accacgtaac cgggctcacc ggggtgatcg gtgctctgcc cgtcggggcc
      961 gtctgggccg ataccgaacc acataccaca ggcgcatcgg ccgacttctt cactcacttg
     1021 gagagtcttg ggatgactgc ttccgctccc gcggtgggtg atcgctggca gctcggtgcc
     1081 ctccacctca ccgtggaggg accggttcga cgctatgcct ctccgaacga ccagtcgatc
     1141 gtcgtcacgg tgacgggcag ctcccgttcg atgctgctgt caggagacat cgagaccttt
     1201 gcccaggctg acttgagtca tctcagggcg gatgtgctca aagtccctca tcaaggggcg
     1261 gccacatcag accccgactg gctggccgag gtgggtgcgg accaggccgt gatctctgtc
     1321 gggcccaacg atttcggaca tcccgtcgat tgggtgatca agaccctgga gtcgtctgga
     1381 gcctcggtac tccgaaccga tctggcaggc gatgtcttgg tcgatctgac cacaccgtga
     1441 gctgctggac aagcgtgtgc agatggcgtg ccctggccac cgtatctgca cacgctcacc
     1501 ctcttggagc cgaggtccac tggtgccagg atccgcacag aatggcgcca tgaagcatgt
     1561 agtcctggtc ggggcgatgg gggctggtaa gtccacagtt gggcggcggg tcgccgagcg
     1621 tcttgggctc cgtttgtcga ttctgacgat gagctcgaag aggcccatgg tgagactggg
     1681 gctgagatcg cctcgcgggc cggtgtcgac cggttgcacg ccctcgagct tcaggtgctc
     1741 catcgaatga tcgacagcca ggacaggagc gtcatcgcac cggcggccag tgtcgtcgac
     1801 tccgatgagg gtcgttcgat actgacccga aatcaaacga tctggctcga tgcgccagag
     1861 gatgtgctgt cggtccgccg agccgagggc gatcatcgcc gtgaggtggg ccgcgaggac
     1921 gagaaggcat tggagcggag acgacgccca ttctgggagc gcatcgcagc ggtcaggatc
     1981 gacacttcgg ggtccgtggg ggaggtcgta gagaccgtgc ttcgggagat tcgtcggatc
     2041 gaggctggtc agaaccgctc gtagagcctg acgtgacgcg tgatgccttg tgttagccat
     2101 ctcgggtcaa cgttttcgcc cttggctcgg tgaaccgaca gcccttctaa cctcgcccgg
     2161 tgcccgacgc tcttcgtctc ggatcccggg aactgcccct gggcggcgtt acccatgtca
     2221 tgggggtgat caacgtctct cccgaatcga agaacgccca caccgtcgcc cgctccatcg
     2281 acgaggctct gctcatggcg gaccgctacc gagcctgggg agcggcaatt gtcgatgtcg
     2341 gcgggcaatc cagccatctc gacaacgaga ccattgccgc ttccgatgaa gtctccagag
     2401 ttgtccccgt gatcgaggcg ctgtgcgccg aaggacacat cgtctctgtc gacacctgga
     2461 aatccgatgt cgcccaggcg gcgatcgagg cgggcgccgt catcgtcaac gacactggtg
     2521 gcctggcggc gccagagatg agagaagtcg tggccgactc cgatgtcgcc gcggtggccg
     2581 tctacatcga agcggccaat cctcatgcgg tgaatgaagt ccagatcagg gacgacaagg
     2641 ccgagcacac cgccaaccgc tttcgttcgc tgctcgcgga gctgacaaag gcgggagtgg
     2701 cgaatgtgat cctcgacccg gggatcgctc tcaactaccg gggtgactat gagacatact
     2761 ctcgaatgca gctccaggtg atcgccggct ctgaccactt gaaggctctt gggcgacccg
     2821 tcctgatccc gatcccccga aagcgcgacg gacaccggtt ggcggtgtac atgggactgg
     2881 ccttggaaca cggcgctgac atcattcgcg tccacgacgt cgcaatggct tgcgatctgg
     2941 tcgacctttt tggtcgtagc gctccgctac gttgatgcca tgacgttgat ggtggtggcc
     3001 ggaccatcca gcccgggccc gggtgagcga gagcagatgt tggaactggc cgccaagcac
     3061 ctggtcaccc agggtgtcgc ccgcgaccag gtcgtcagaa tcgatgtccc cgggcgggga
     3121 gcgggcgaag acggagacgg gagtctgcgc gccgagctgg agccgatgat tcctctcctt
     3181 cagtcgggct cgctcttcgg agatgctcag gggctcgagc tgatggatgc ccagaacctg
     3241 cagaagtccg agttggagac gctcatcgct cttctggacg gagcagacct ctccgccata
     3301 gaggtggttc tcatctatgg gggtgccgtt cccaaggcgg tcaaagacct cggtggtcca
     3361 tcggtttcgg tcaagaagat gtgggagcgt caagcgggcc agtggctaga agccgagatc
     3421 agagagcgtg gcctcgagct cgacaagtca gttgtccagg cgctgatcca gaggttcgga
     3481 agtgacaccg ccagtctcgg ccaggtgttg gatcaattgg cggagacacc ggggaagatc
     3541 accgccgagt tggtgctcga ccgtttcaag aaccgacccg acgagcccac
//
LOCUS       DASUTA010000216         3232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1228601, whole genome shotgun sequence.
ACCESSION   DASUTA010000216 DASUTA010000000
VERSION     DASUTA010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228601"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VFZ80_04345"
     CDS             <1..242
                     /locus_tag="VFZ80_04345"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HEX6286691.1"
                     /translation="LWRQTWVDSAGGYIVLSGSYDGETMELRTEAAEREGETVVQRMV
                     FSDIDADSLQWSWQGSRDGGATWNDLWNISYRRRS"
     gene            complement(265..1479)
                     /locus_tag="VFZ80_04350"
     CDS             complement(265..1479)
                     /locus_tag="VFZ80_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6286692.1"
                     /translation="MPIDRLDQTLAAELQELRQTGTDKGAESVVTEVVPPRSGVGPRY
                     RLAGDDREFLRMNSNSYLGLSSHPAVLDAEEAATRRFGAGPGAVRFISGTYQHHLDLE
                     NRLAGFHGREAAVIYSSAYAAVVSTLSSLITPETVVLSDELNHNCIINAIRMSRPADR
                     AVYPHLDTEALDRLLAEATDRGSRALVVTDGVFSMRGDYADLRAISSVADEHDSAFAE
                     NVVVVVDDSHGVGAYGSTGRGTEEVTSGQADVLIGTLGKAFGVNGGYVVGSETVTTYL
                     RERGPMYIYSNPITPGEAAAADMAVSILDSPEGAELLAHLRSMTMRFESGLVEAGFET
                     IPGPHPVVPLMVRDTETTAALVAHLFENGVLATGLNYPVVPKGDEEIRFQVNADHTVA
                     DIDYVLTVLDGF"
     gene            1435..2412
                     /locus_tag="VFZ80_04355"
     CDS             1435..2412
                     /locus_tag="VFZ80_04355"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6286693.1"
                     /translation="MELGGECLVQAVDWHRRTHTRYGGRANYVRQAMTRLGYVGTGIM
                     GHGMASNLIAAGHAVTVWNRTRSKTDDLEAKVVDTPQDVGPESDVVFVCVSDTPDVEE
                     VVFGDYGVIHGMTSGDVLVDHSTISPVATAGFAAQAAELGVEWLDAPVSGGSEGAQRG
                     TLAVMVGGDSAALERVRPFMEAYSTSIVHVGEEPGSGQMVKAVNQVLVVLNQLAASEA
                     LMLADAAGLDLEKTLAAVEGGAAGSWMLSNRGPQMIERDWSPGFTIDLQQKDLRLVLE
                     AADEVGATLPGTALVFQLYRSLQTRGLGSEGNHALVKALENLTGKELGA"
     gene            complement(2473..2919)
                     /gene="bcp"
                     /locus_tag="VFZ80_04360"
     CDS             complement(2473..2919)
                     /gene="bcp"
                     /locus_tag="VFZ80_04360"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEX6286694.1"
                     /translation="MSQTVEFKLSNQTGDQVSSEDFAGRKYVMFFYPRAMTPGCTTES
                     CDFRDAYAEFRDAGYEVVGVSPDPPSRNAEFKEQERLTFDLLSDEDHALAESLGAWGE
                     KKMYGKTTEGLIRSTFVIDEKGEVEKAYRNVRAKGHVERVKSDLLG"
     gene            complement(2930..>3232)
                     /locus_tag="VFZ80_04365"
     CDS             complement(2930..>3232)
                     /locus_tag="VFZ80_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEX6286695.1"
                     /translation="GALWLVMLAFNIDLNVWEAIAVYAVSQLVGGLPGTPGGLGVTEF
                     GMGLVLAAYGYPASTTVAPILVYRIVSYWLPAGLSLLAGGSTFLRSDEAKAVADAD"
BASE COUNT          594 a          996 c         1076 g          566 t
ORIGIN      
        1 gcctctggag acagacctgg gtcgacagcg ccggcgggta catcgtcctc agcggatcct
       61 acgacggcga gacgatggag cttcggaccg aggctgcgga gcgagaaggc gagacagtgg
      121 tgcagcgaat ggtgttcagc gacatcgacg ctgactcatt gcagtggtcc tggcagggaa
      181 gccgtgacgg cggtgcaacc tggaacgacc tctggaatat cagctaccgg cgacggtcct
      241 aggccagacg ctgggaggac cagctcagaa gccgtcgaga acagtcagga cgtagtcgat
      301 gtcggccacg gtgtggtcgg cgttgacctg gaatctgatc tcctcgtccc ccttgggcac
      361 caccggatag ttgaggccgg tcgccagcac gccgttctcg aagagatgag ccaccagcgc
      421 agcggtcgtc tcggtgtcgc gcaccatcag cggtaccacc ggatgtggtc cggggatggt
      481 ctcgaacccg gcctcgacga gcccagattc gaaccgcatc gtcatcgatc tcagatgtgc
      541 caacagttcg gcgccttccg gactgtcgag aatcgacacg gccatgtcgg cggctgcggc
      601 ctcaccgggg gtgatcgggt tggagtagat gtacatgggg cctcgctcgc ggaggtaggt
      661 cgtgacggtc tcgctcccca cgacatatcc tccgttgacc ccgaatgcct ttcccaaggt
      721 gccgatcaac acgtcggcct gtcctgacgt cacctcctca gtgccccgac cggtggatcc
      781 atatgccccg accccgtgtg agtcgtcgac gacaactacc acattctcgg cgaaagcaga
      841 gtcgtgctcg tcggcgacac tgctgatcgc ccggaggtcg gcgtagtcgc cgcgcatcga
      901 gaagacgccg tcggtgacga cgagagccct ggatcccctg tccgtcgcct cggccagaag
      961 acgatcgagc gcctcggtgt ccaggtgcgg gtagacggcg cgatcggcgg gacggctcat
     1021 tctgatcgca ttgatgatgc agttgtggtt gagctcatcg gagagcacca ccgtctccgg
     1081 ggtgatcagc gaggacaggg tggagacgac cgcggcgtac gcggaggagt agatgacggc
     1141 ggcttccctg ccatggaatc cggcgagacg gttctccagg tcgagatggt gctgataggt
     1201 ccccgagatg aaacggaccg ctcccgggcc ggccccgaaa cgacgtgtgg ccgcttcctc
     1261 ggcatcgagc accgcggggt ggctggatag ccccagatag ctgttggagt tcatgcggag
     1321 aaactcgcgg tcgtccccgg cgagtcggta tcgcggtccc acgccggatc gaggcggaac
     1381 gacctcggtg acgacggact ctgccccctt gtcggtcccg gtctgccgca gctcttggag
     1441 ctcggcggcg agtgtctggt ccaggcggtc gattggcatc gccggacgca cactagatac
     1501 ggaggccgag ccaactatgt tcggcaggcg atgacacgtc tcggatacgt aggcacagga
     1561 atcatgggcc atggaatggc ctcgaacctc atcgcggccg gtcacgcggt gacggtttgg
     1621 aacagaaccc gctccaagac cgacgacttg gaggcaaagg tggtggacac ccctcaggat
     1681 gtcggcccgg agtcggatgt cgtcttcgtg tgcgtatccg acacccccga cgtcgaagag
     1741 gttgtgttcg gcgactacgg ggtcatccac gggatgacgt ccggcgacgt cttggtagac
     1801 cactcgacga tctctccggt ggcaaccgcc ggcttcgccg cccaggcggc cgaactcgga
     1861 gtggagtggt tggacgcgcc ggtctcaggc ggatcggaag gcgcccaaag agggactctg
     1921 gccgtcatgg tcggcggcga ctctgcggcg ttggagaggg tgcggccctt catggaggcc
     1981 tactcgacct cgattgtcca cgtcggcgag gaaccgggct cgggacaaat ggtcaaggcc
     2041 gtgaatcaag tgttggtggt gctcaatcag ctggcagcgt ccgaggcgtt gatgctggcc
     2101 gacgcggccg gcctcgacct cgagaagacc ttggcggctg tcgagggagg ggcagctggc
     2161 tcgtggatgc tctccaaccg cggaccgcag atgatcgagc gcgactggag ccccgggttc
     2221 accatcgatc tgcagcagaa agaccttcgt ctcgtgctcg aggctgccga cgaggtggga
     2281 gcgacactcc ccgggaccgc tctcgtcttc cagctctacc gatcactgca gacccgaggc
     2341 ctcggctcag agggcaacca tgccctggtc aaagccctcg agaacctcac cggcaaggag
     2401 ttgggggcct gaaacaccgt tctgtgaacg ccgggtgagc cccacgctca ccggacgttc
     2461 acagaacggt gttcacccca ggaggtcgga cttgaccctt tcgacgtggc ccttggcgcg
     2521 cacattgcga taggccttct cgacctcgcc tttctcgtcg atcacgaagg tggagcggat
     2581 gagcccctcg gtggtcttgc cgtacatctt cttctcgccc catgctccca gtgactcggc
     2641 gagggcatgg tcctcgtcgg acaggagatc gaaggtgagc cgctcttgct ctttgaattc
     2701 ggcgttgcgg gacggcggat cggggctcac cccgacgacc tcgtaaccgg catcgcgaaa
     2761 ctccgcgtat gcgtcacgga aatcacatga ctccgtggtg cagccagggg tcatggctct
     2821 gggatagaag aacatgacgt acttcctgcc agcgaagtcc tcgctcgaga cctgatcgcc
     2881 ggtctgattg gacagcttga actcgacggt ctgggacatc cggacgaggc taatcggcgt
     2941 ccgcgaccgc cttcgcctcg tctgatctga ggaaagtgga cccaccggcg aggaggctga
     3001 ggcctgccgg cagccagtag gagacgattc gatatacgag gatcggggcc actgtggtgc
     3061 tcgccgggta gccgtaggcc gccagaacga gacccatgcc gaactcggtg acgccgaggc
     3121 ctccgggggt cccaggcagc ccgcccacca gctgggacac ggcatagacg gcgatggcct
     3181 cccacacatt gaggtcgatg ttgaacgcaa gcatcaccaa ccacagggcg cc
//
LOCUS       DASUTA010000217         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1099208, whole genome shotgun sequence.
ACCESSION   DASUTA010000217 DASUTA010000000
VERSION     DASUTA010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099208"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFZ80_04370"
     CDS             <1..208
                     /locus_tag="VFZ80_04370"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX6286696.1"
                     /translation="YDIVVRESARGLTLASLETGVPITNAILAVHDHQLALDRAGPGD
                     DNKGVEATNAAVNMATALSTLGAR"
     gene            522..1061
                     /gene="infC"
                     /locus_tag="VFZ80_04375"
     CDS             522..1061
                     /gene="infC"
                     /locus_tag="VFZ80_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX6286697.1"
                     /translation="MNGAIRAKEVRVVAPDGEQIGVKKINEALWLAEQLDLDLVEVAP
                     NAKPPVCRLMDYGRYKYEQSVKEREARKKQTRTQIKELRMRVRIGEHDFEITRRKTRE
                     FLDDNDKVKVTIRFRGRERERPEFGRDLMDRLAAEVAEYGTVEQAPKIEGASMTMVLA
                     PLPKSQRPKSREQHKDEEE"
     gene            1071..1265
                     /gene="rpmI"
                     /locus_tag="VFZ80_04380"
     CDS             1071..1265
                     /gene="rpmI"
                     /locus_tag="VFZ80_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005319593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX6286698.1"
                     /translation="MPKMKTHSGAKKRIKITGTGKYMRRQSGRGHLRLAKGKATFNRR
                     KGMVELAPGDAKVIEKQLGK"
     gene            1277..1627
                     /gene="rplT"
                     /locus_tag="VFZ80_04385"
     CDS             1277..1627
                     /gene="rplT"
                     /locus_tag="VFZ80_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX6286699.1"
                     /translation="MARVKRAVHAKKSHRKVLDRAKGYKGARSRRFKTANEQVMHAMQ
                     DSYRDRRARKREFRKLWIARINAAARQNGTTYSQFIAGLKAAEIEVDRKMLAEMAVND
                     PDAFAQLVEVAKAS"
     gene            1648..2394
                     /locus_tag="VFZ80_04390"
     CDS             1648..2394
                     /locus_tag="VFZ80_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX6286700.1"
                     /translation="MEPVRTHRAKTVVEAARLHRARDRKERGLTLVEGPQLLADVAAA
                     GGEVVEVFSTQSPEGEPPRAVLPTLVDQRALERIAGTKSPRGPVAVVEIPTEWLDSSR
                     NLLVSVGVSDPGNVGTLVRTAASFGWGFAYTDGSADPWAPKTIRAGAGGQFQTSVSRI
                     GSISELGEWETVAAVTRDGAEPDSLPAGRYALLIGEEAHGLPEAFVAAARHRITIATP
                     GATESLNAAVAAGIAVYALSRGSGESTRGV"
BASE COUNT          552 a          774 c          812 g          386 t
ORIGIN      
        1 ttacgacatc gtcgtcaggg agtcagctcg agggctcacc ctggcatcac tggaaacggg
       61 cgtcccgatc accaacgcca tccttgctgt gcacgatcac cagctggccc tcgaccgtgc
      121 cgggccaggc gacgacaaca aaggcgtcga agccacgaac gccgccgtca atatggccac
      181 tgcgctcagc acactggggg cccgctgaga cacttcgata ttggctcgtc ccggggcact
      241 ggtaacctga tcgggacaac agatttgcaa gagcggaggt gctgcctccc cacccgacca
      301 cgcaggacgc caacgcttcc ggctgtgagt cgaggtccac aatcagacgg gtcgatgacc
      361 cgtgatcgat cgtattggca gcagatctcg gttcgctgcc gcaaagggca gcagcaggaa
      421 tcacctgctg cttttttgat gtgcagtgca catcggttgt tgggacagaa cgcaggaaaa
      481 ggctgcaatc agcaggaggt aacagcatcg cagagctgcg cgtgaacgga gcgatccggg
      541 caaaagaggt ccgggtggtc gcccccgatg gagagcaaat cggagtcaag aagatcaatg
      601 aggccctatg gctcgcggag caactcgatc tagacctggt cgaagtagcc cccaacgcca
      661 agccaccggt ttgccggctg atggactacg gacggtacaa gtacgaacag tccgtgaagg
      721 aacgtgaggc tcggaagaag cagacccgaa cccagatcaa agagcttcgg atgcgagtcc
      781 ggatcggtga gcacgacttc gagatcaccc gtcgcaagac gcgtgagttc ctcgatgaca
      841 acgacaaggt gaaggtgacc atccggttcc gcggtcggga gcgggaacgt cccgaattcg
      901 gccgagatct gatggatcgc ctcgccgcag aagtggccga atacggcacc gtggagcagg
      961 cacccaagat cgagggcgct tcgatgacca tggtgctcgc ccccctgccc aaatcgcagc
     1021 gtcccaagag cagagagcag cataaggacg aagaggaata ggaggatcct gtgcccaaga
     1081 tgaagacaca cagtggcgcc aagaagagaa tcaagatcac cggaaccggc aagtacatgc
     1141 gtcggcagtc cggtcgcggc cacttgcgac tcgccaaagg caaggcgacc ttcaatcgtc
     1201 gcaagggcat ggtcgagctc gctccgggcg acgccaaggt catcgagaag caactgggca
     1261 agtaggagac cgacacatgg cacgtgtaaa gcgcgcagtt catgcgaaga aatcccacag
     1321 gaaggtcctc gaccgggcca agggctacaa gggggcccgc agccgccgct tcaagaccgc
     1381 caacgagcag gtcatgcatg ccatgcagga ctcgtatcgt gaccggaggg cccgcaaacg
     1441 cgagttccgc aaactgtgga tagcccgcat caacgctgca gcccggcaga acggcaccac
     1501 atacagccag ttcatagccg gcctgaaagc agcggagatc gaggtagacc ggaagatgtt
     1561 ggccgagatg gcagtgaacg atccggacgc cttcgcccag ctcgtcgagg tcgccaaagc
     1621 cagctgagcg aatcgatagt cgatccaatg gagccggtcc gcactcaccg agccaaaacg
     1681 gtggtggagg cggcccggct tcatcgcgcc cgggaccgca aggaacgggg attgaccctc
     1741 gtcgaggggc cgcagctgct ggcggatgtg gcggcggccg gtggcgaggt ggtcgaggtg
     1801 ttctcgacac agagcccgga aggagagcca cctcgggctg tcctgcccac cctggtcgac
     1861 caacgcgccc tcgagcgcat cgccggaacc aagtcgcctc ggggaccggt cgccgtggtg
     1921 gagatcccca ccgagtggct cgactcctca cgcaatcttc tcgtttcggt cggcgtctct
     1981 gaccccggga atgtgggaac cctggtccgt accgcagctt ctttcggctg gggcttcgcc
     2041 tacaccgacg gatctgccga cccgtgggct ccgaagacga ttcgggcggg agccggaggc
     2101 caattccaga cgtccgtctc gaggatcgga tcgatttcag agctggggga gtgggagacg
     2161 gtggctgccg tcacccggga tggtgcggaa ccggactcgc tcccggctgg gcgatatgca
     2221 ctcctgatcg gtgaggaggc ccatggtctg cccgaggctt tcgtggcggc ggcccgacat
     2281 cggatcacca tcgccacgcc gggagcgacc gagagcttga acgcagcggt cgccgccggg
     2341 atcgccgtct acgccctctc tcgtggttcc ggggaatcca cacgcggggt ctaacctccc
     2401 gccctcatgg acccgataga gacgctcgac aaggcgatcg ccgatgctcc tgcttcggtc
     2461 gactcaacag cctcgctcgc cgagctcgag aaggtcgaat cagagctgct cggaaaggac
     2521 tccg
//
LOCUS       DASUTA010000218         3011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_710106, whole genome shotgun sequence.
ACCESSION   DASUTA010000218 DASUTA010000000
VERSION     DASUTA010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710106"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VFZ80_04395"
     CDS             <1..311
                     /locus_tag="VFZ80_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286701.1"
                     /translation="YADRVEWADSLDGALALVFGPGGDDGDGDGGDGDGGEVPGDTST
                     LEELIEDAEDAFTAADAALRNGDLASYQQWVREARRILLEIADLVAGEEPNASLLHPL
                     "
     gene            347..574
                     /gene="infA"
                     /locus_tag="VFZ80_04400"
     CDS             347..574
                     /gene="infA"
                     /locus_tag="VFZ80_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX6286702.1"
                     /translation="MAKDSDEYIRMRGTVTKMLPDSTFEVKLESGFELLAKPSGRMRR
                     GRGIRVLPGDEVDVEVSTYDPTRGRIVWRYR"
     gene            657..1013
                     /locus_tag="VFZ80_04405"
     CDS             657..1013
                     /locus_tag="VFZ80_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6286703.1"
                     /translation="MRIGQIILRVADMDESIRFWSSAVGFEVRMRGASFSFLDGGSAQ
                     LTLNQVEEMKDDESLTEIVIEVDDITSAYEELSNRGVPFEVEPRAVMSDGGRELWATH
                     FRDPDGHLASIVGWIG"
     gene            complement(1010..1210)
                     /locus_tag="VFZ80_04410"
     CDS             complement(1010..1210)
                     /locus_tag="VFZ80_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286704.1"
                     /translation="MDAVVETTEEGVTLTHIGITVCRGPGQHRLLLTTPYHQAGQIIE
                     IPKSRIDKVTRLENGHTNGVSR"
     gene            complement(1239..1700)
                     /locus_tag="VFZ80_04415"
     CDS             complement(1239..1700)
                     /locus_tag="VFZ80_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX6286705.1"
                     /translation="MTDTPALTDPRLENLEHEVVRHGTVSSLEEAATARGVAPGQVIK
                     TMVVRRGADDYLFVLVPGDQNISWPKLRGHLAVNRISMPDADEAKSVTGYERGTITPF
                     GSTRAWPVLADRSLTGVVSIGAGAPGVSLTVDAESLIETLQATVADITDPV"
     gene            complement(1697..2137)
                     /locus_tag="VFZ80_04420"
     CDS             complement(1697..2137)
                     /locus_tag="VFZ80_04420"
                     /inference="COORDINATES: protein motif:HMM:NF042760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX6286706.1"
                     /translation="MSEGTDRIYGDLPPFHRRITSIEKFGTVLTCMDGRPQRKVADYL
                     TTSFGVRHLDLITTAGAVRHLAEETDETHRLLHNLALSTSNHGSRQIAVVAHHDCAGN
                     RVADNRQKLQVSEAVARIRNIYPDAEVTGLWLGEQWIVERVVPT"
     gene            2466..>3011
                     /locus_tag="VFZ80_04425"
     CDS             2466..>3011
                     /locus_tag="VFZ80_04425"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6286707.1"
                     /translation="MSHRPSRRLTPRQLLVDITPLRRSIPFRWLFGGLSLSWMGRQLT
                     VVAVPFQVYQLTGSTFLVGALGIAQLVPLLLASIVGGAVVDSVDRRRLLVIAQLASLG
                     TAIGLAVNASLDRPSVWLIFALSAVNAAFSAIDSPARSASLPTLVGRELLPASLAITQ
                     TVANVAKTVGPAVGGLLIAQTS"
BASE COUNT          498 a          932 c          958 g          623 t
ORIGIN      
        1 tctatgccga ccgggtggag tgggccgact ccttggacgg tgcccttgcc ctcgtttttg
       61 gaccgggagg cgacgacggt gacggtgacg gaggggatgg ggacggtggg gaggttccag
      121 gtgacacgtc cactctcgag gagctgatcg aggacgccga agacgccttc acggctgccg
      181 atgccgctct tcgcaacggc gacctggcta gctaccagca gtgggtccgg gaggcgcggc
      241 gcatcctcct ggagatagca gacctcgttg ccggggagga gcccaacgcc tccttgctac
      301 acccgctcta ggactacacc gaccgtatcg cctaacctga gagagaatgg ccaaagactc
      361 cgacgaatac atccgcatgc gtggcaccgt caccaagatg ctccccgact ccaccttcga
      421 ggtgaagctg gagagcggtt tcgagctact agccaagcca tcggggcgga tgcgccgggg
      481 aaggggcata cgtgtccttc cgggcgacga ggtcgacgtt gaggtctcga cttacgatcc
      541 gactcgggga cgcatcgtct ggcgctaccg ctgaccactc aaaccccagt cgaagatcgg
      601 cccctccctc gagggggttt catcatttct cccatcgatc tgattaggtt cacgtcgtgc
      661 gcatcggcca gatcatcctt cgcgtagcag acatggatga gtcgatccgg ttctggtcga
      721 gcgctgtcgg gttcgaggtg aggatgagag gagcgagctt ctcctttctc gacgggggca
      781 gcgcccagtt gacgctcaat caggtcgagg agatgaagga cgatgaatct ctcaccgaga
      841 tcgtgatcga agtcgacgac atcacgagcg cgtatgaaga gctctcgaac cggggcgtcc
      901 cattcgaagt cgaaccccgg gccgtcatgt ccgacggtgg ccgtgagctg tgggccactc
      961 acttccgaga ccccgacggc catctggcga gcatcgtcgg ctggatcggc tagcggctca
     1021 cgccattcgt gtgtccgttt tcgaggcgag tcaccttgtc gatccgcgat ttgggtatct
     1081 cgatgatctg tcctgcctgg tgatacggcg tggtcaatag cagccggtgc tggccgggcc
     1141 ccctgcacac cgtgatcccg atgtgggtca gggtcacccc ttcttcggtt gtctctacca
     1201 cggcgtccat ggcaaaccat taccccggcg ctgtctcctc aaaccgggtc ggtgatgtcg
     1261 gcaaccgtgg cttgaagcgt ctcgatcagg ctctctgcgt cgacggtgag cgagacgccc
     1321 ggcgctcctg cgcctatgga aacaaccccg gtcaacgacc gatcggccag gacgggccaa
     1381 gcacgagtcg aaccgaaggg tgtgatggtc cctcgctcgt atccggtcac cgatttggcc
     1441 tcgtcggcgt caggcatcga gatacggttc acggccagat ggccccggag cttgggccac
     1501 gagatgttct ggtcccccgg gacgaggacg aacaggtagt cgtcggctcc tcggcgcacc
     1561 accatggtct ttatcacttg gccgggcgca accccacgag ctgttgcggc ttcctcgagc
     1621 gacgagaccg taccgtggcg aacgacctca tgttccagat tctcgagcct gggatcggtg
     1681 agtgcgggcg tgtcggtcat gtcggcacga cgcgttcgac gatccattgc tcgccgagcc
     1741 acagcccggt gacttcggcg tcggggtaga tgttgcggat gcgggccacc gcctcgctca
     1801 cttgcagctt ctgtcggtta tcggctaccc ggttgcctgc gcagtcgtgg tgggcaacca
     1861 cggctatttg tcgtgagcca tgattgctcg tcgacagtgc gagattgtga agcagcctgt
     1921 gagtctcatc cgtctcctcg gcgaggtgcc tcactgcacc ggcggtggtg atcaggtcca
     1981 gatgacgcac gccgaacgat gtggtgaggt agtcggccac cttgcgctga ggacggccgt
     2041 ccatacacgt gaggacagtc ccgaatttct cgatcgatgt gattctacgg tgaaatgggg
     2101 gaaggtcccc atagattctg tcggtgcctt ccgacatgat cacctcatgg agatactcac
     2161 caacgttggg gcgatggtga caggggtggt gacggttctg gccggtctct tctctctggc
     2221 cacgatcctc ttcgacgatc tcatctgagc ttcaggtccg cttcggtcgg cttttcggga
     2281 cagttgtcgg cccaggactt gcaacgacca tgtcgccatc cgaggcacgg ccacggccgt
     2341 taggcagtct tcgcgggggg cgaacatgtc cattgtctgc atagaggttg gaacccactg
     2401 cgcacctggg ggacgtgggt acagtcgccg gtcgtggtcc actacctccc cggtcgaccc
     2461 ggctgatgtc gcacaggcct tcgaggcggc tgacaccgcg tcagcttctc gtcgacatca
     2521 cacctctgcg gcggtcgatc ccgttccgtt ggctgttcgg tgggctctcc ctctcgtgga
     2581 tgggtcgaca gttgactgtc gtcgccgtcc cgttccaggt gtatcagctg acgggatcga
     2641 cgtttctggt cggggctctg ggcatcgccc aactcgtccc gcttctcctc gcctcgatcg
     2701 ttggaggcgc cgtggtcgat tccgtcgacc ggcgcaggct tctcgtcatc gcgcagctgg
     2761 cgtccttggg caccgccata ggtctggcgg tcaatgcctc cctagaccgg cctagcgtct
     2821 ggctgatctt cgccctgagt gcggtcaatg ccgcattctc tgccatcgac agcccggcac
     2881 ggtccgcctc gctcccgact ctcgtgggga gggagctgtt accggcgtcg ctggcgatca
     2941 cccagacggt ggccaatgtg gccaagacgg tgggacctgc tgtcggcgga ctgctcatcg
     3001 cacaaaccag c
//
LOCUS       DASUTA010000219         2468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1359017, whole genome shotgun sequence.
ACCESSION   DASUTA010000219 DASUTA010000000
VERSION     DASUTA010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359017"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VFZ80_04430"
     CDS             complement(<1..858)
                     /locus_tag="VFZ80_04430"
                     /inference="COORDINATES: protein motif:HMM:NF042569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase kinase/phosphatase AceK
                     regulatory subunit"
                     /protein_id="HEX6286708.1"
                     /translation="MASSLSDSRLSNLTAARVLEGFEDYMSRFNAFNKRAPTRFAQAD
                     WRGMQADALGRLKIYSHVVKTVVDDIGRTLGDRIDDPLLWIAIKAVYSSLITDRQDWE
                     LAETFHNSVTRQVFDTVGVDSSIEFVDTDFDSPPSPSHEAVYVIFERQDTTADLVSRI
                     LSHFMQDTPFKRLEDDASAVASRIEERLAERGGLPVVGRAEMVRSVFYRGQSAYIVGL
                     LYAGSVRLPMVLSLRHEVGGVVVDAVLLTEDEVSVLFSFTRSYFHVEVTRPYDLVRFL
                     RRLMPRKRIA"
     gene            complement(886..1380)
                     /locus_tag="VFZ80_04435"
     CDS             complement(886..1380)
                     /locus_tag="VFZ80_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HEX6286709.1"
                     /translation="MKLPDDLARAFNDQITMELASSVAYLQMAAYFDGENLTGMSSWM
                     KAQAEEEKVHAHRFLQFVLDRGNPVKIGSIEAPRSDFESVEQVFETALAQEQRVTMAI
                     HDIYRLASDGGDLASFPFLQSFIEEQNEEEATVETILERVRLAGGESSAILLLDSELG
                     GRTA"
     gene            complement(1496..>2468)
                     /locus_tag="VFZ80_04440"
     CDS             complement(1496..>2468)
                     /locus_tag="VFZ80_04440"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX6286710.1"
                     /translation="SALDTRHAPSQGSLEKAAAAGLQDSMPRAALLSIHARVSETGPD
                     TWAKSPFVQVWGPRFSAYVIAERDVALFTLGRLSDEPKRRQQAEEIAHRLEAVLGEDR
                     MDVRDAARVMGVHDNTLRYAAPTGRFLIYWDGARQPLIWSIPAPTIEPRDARSELLRR
                     YLQVFGPATAASFASWAGIRPQPAAAAFGRLGTELVTVQTPIGEGWIRAEDESAFRSA
                     EGAISTRLLPSGDTFYLLQGADRELLIPDAEQRARLWTSRVWPGAVIWDGEIVGTWRR
                     SGHKLTIELWQPVTGGGRDAIAAEAVSMPIPGIEREITVIWEDGGSP"
BASE COUNT          449 a          779 c          783 g          457 t
ORIGIN      
        1 ggcgatccgc ttgcggggca tgagtctgcg caagaaccgg accaggtcat aggggcgggt
       61 gacctcgacg tggaagtagg agcgggtgaa gctgaagaga accgatacct cgtcctcggt
      121 gagcagcact gcatccacca cgacgccacc cacctcgtga cgaagggaca acaccatggg
      181 caaccgcacc gagcccgcat acaggagccc gactatgtag gcggattgac cccggtagaa
      241 gacggagcgc accatctcgg cccgaccaac gacgggcagg cctccgcgct cggcgagacg
      301 ttcttcgatt cgggaggcga ccgcagaggc gtcgtcttcg agacgcttga agggggtgtc
      361 ctgcatgaag tggctcagga tgcgggagac gaggtcggct gttgtgtctt gacgttcgaa
      421 tatcacgtag accgcctcgt gagatgggct cggcgggctg tcgaagtcgg tgtcgacgaa
      481 ctcgatgctg ctgtccactc ctacggtgtc gaagacctgt ctcgtcaccg agttgtggaa
      541 cgtctcggcc agctcccaat cctggcggtc ggtgatcaga ctggagtaga cggccttgat
      601 cgcgatccac aacaacgggt cgtcgatgcg gtccccgagt gtgcgcccta tgtcgtcgac
      661 aacggtcttg acgacgtggc tgtagatctt gagccgcccc aatgcgtcag cctgcattcc
      721 ccgccagtcg gcttgggcga acctcgtcgg agcacgcttg ttgaacgcgt tgaaacggct
      781 catgtagtcc tcgaagccct cgaggacgcg agcagcggtc aggttggaga gccggctgtc
      841 ggagagcgac gaggccatca gtgctctcgg ccgaccgtgg caccgctagg cggtgcgacc
      901 gcccagctca ctgtcgagaa gcaagatcgc gctcgactcg cccccggcga gtcgtactcg
      961 ctccaggatg gtctccaccg tggcctcttc ctcgttctgc tcttcgatga agctctggag
     1021 aaaggggaag ctggccaggt cgcctccgtc agaggccaac cggtagatgt cgtggatcgc
     1081 catggtcacc cgctgttcct gagccagtgc cgtctcgaac acctgctcga cgctctcgaa
     1141 gtctgagcgc ggcgcctcga tggagccgat cttcaccggg tttccccgat ccagcacgaa
     1201 ctggaggaag cggtgggcat gcaccttctc ctcctcggcc tgtgccttca tccacgagct
     1261 catcccggtg aggttctcgc cgtcgaaata tgcagccatc tgcagatagg ccaccgatga
     1321 ggcgagttcc atggtgatct ggtcgttgaa ggctctggcg aggtcgtcgg gcagtttcat
     1381 actctttgtc tcctgtctag gaaggcatcg taaacatcgg gataccggtc gttgagttca
     1441 cacgcagtgc gcaccaacgg atcatgcctc gagggaagcg agatgcctcc atgtctcatg
     1501 gtgatccgcc gtcctcccag atgacagtga tctcccgttc gatccccgga ataggcatcg
     1561 agactgcttc ggctgcaatg gcatcccgac cgcctcccgt gactggttgc catagctcta
     1621 tcgtcaactt gtggcccgac cgtcgccacg ttccgacgat ctccccgtcc cagatcactg
     1681 ctccgggcca cacccgagac gtccagagcc gtgcccgctg ctcggcatcg gggatcagca
     1741 gctcacggtc cgcaccctgg agcaggtaga aggtatcgcc gctcggcagc aggcgggtcg
     1801 agatggcccc ttccgccgag cggaacgccg attcatcctc ggcccggatc cagccttctc
     1861 cgatcggggt ctggacagtg accagttctg tgccgagcct cccgaaggcg gccgcggctg
     1921 gctgggggcg gatcccggcc caggaggcaa aggaggcggc cgtggcagga ccgaagacct
     1981 gcaggtatcg gcgcagcaac tccgagcgcg cgtcccttgg ctcgatcgtc ggtgcgggga
     2041 tgctccagat gagcggctgt ctggcaccat cccaatagat gaggaacctg ccggtgggag
     2101 ccgcgtaccg cagagtgttg tcgtgcacgc ccatgacccg agcagcatcg cgaacgtcca
     2161 tgcggtcctc cccgagaacg gcctctagcc ggtgggcgat ctcttcggct tgttgtcggc
     2221 gtttcggctc gtccgacaga cggccaagtg tgaatagggc cacgtcgcgc tcagcgatca
     2281 catacgcact gaagcggggg ccccagacct gaacaaaggg tgattttgcc cacgtatcag
     2341 gaccagtctc cgacactcga gcgtggatcg acaacagagc cgccctgggc atgctgtcct
     2401 gaaggccagc tgcggccgcc ttctcgagag agccctgtga gggtgcatgc ctcgtgtcca
     2461 gagccgag
//
LOCUS       DASUTA010000220         2213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_839505, whole genome shotgun sequence.
ACCESSION   DASUTA010000220 DASUTA010000000
VERSION     DASUTA010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839505"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..>2213)
                     /locus_tag="VFZ80_04445"
     CDS             complement(137..>2213)
                     /locus_tag="VFZ80_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX6286711.1"
                     /translation="LGLLGTLPEHPHFDVGREVFGNVPDAAVLVFYVGLAGFLWLTFH
                     LFAQRAVSWQQGGEDRRTGHLGRRVARLVEGLTMRTLMRDPRAGVMHSMIYFGFIVLF
                     LGTVTLEIDHLMPANLKFLHGNFYLGYSAVLDLAGLVFLGGLALAVVGRYVVPAWRIR
                     SKTRPEDALILSVLALIGITGLLIEAARISLAGRPDFEVWSFLGYPLSYLFAQSTAGD
                     WHLFLWVSHAVLFVVFLVVLPLTKLRHMFTSPVNMFLSVNERPKGAMRPMPNLMEAED
                     IETIGASVATDLTWKQIFDTDACTICGRCTSVCPANITGKPLDPREMVLKSGEVAANL
                     AGVAPPVSTTMVLEAAGSLFDRIRPEEVFACTTCRACDEICPVDIGILDRILDMRRYL
                     TLMESEFPSELGKAFVAMENQSNPWGLSQQARADWTSELDFPIPMFGQDGKDTAEYLW
                     FVGCAGSFDDRNTATSVALARLLNEAGIDFAILGQRELCNGDPARRSGNEFVFQQLAL
                     QNIETFEELGVKKIITQCAHCFNALGNEYPQFGGNYEVVHHSQMLAELLSQGRLSEPR
                     KANGEAKKIAYHDACYLGRHNDVYLAPREVVGGSGNDVVEMPRHGTRGLCCGAGGARF
                     WMEEQTGKKVNNERAEEALATGADEIAVACPFCFVMLDDGVKELGRDDVPVRDIAMIL
                     ADGLKGSVG"
BASE COUNT          434 a          716 c          694 g          369 t
ORIGIN      
        1 gaggggccgc tcgccgcgca gcccgccacg gtcaacacca gcagacaaat gacggatgag
       61 cggacgatgg tggaaggccg atcccacaac gcctgtcacc tgtctcgtgg ccacggtgca
      121 gactcgggcg acgacatcag ccgaccgagc ccttgagacc atcggcgagg atcattgcga
      181 tgtcacgcac cgggacatca tcgcgcccca attccttgac tccgtcgtcg agcatcacga
      241 agcagaacgg gcacgccacg gcgatctcat cggctccagt ggcgagcgct tcctcggcgc
      301 gctcgttgtt caccttcttc cccgtctgtt cctccatcca gaacctggcc ccgccggctc
      361 cacagcacag accacgggtg ccatgccgcg gcatctcgac gacgtcgttg cccgagcctc
      421 cgacgacctc acggggggcg agatagacgt cgttgtggcg gccgagatag caagcgtcgt
      481 gataggcgat cttcttggcc tcgccattcg ccttcctcgg ctcgctgaga cgaccctgac
      541 tgaggagctc ggccagcatc tgactgtgat ggaccacctc gtagttgccg ccgaactggg
      601 gatactcgtt gcccagcgcg ttgaagcaat gggcacattg ggtgatgatc ttcttgacgc
      661 ccaactcttc gaaggtctcg atgttctgca gcgcgagctg ctggaacacg aactcgttcc
      721 cggagcgtcg ggcggggtcg ccattgcaca gctctcgttg gccgagaatg gcgaagtcga
      781 tgccggcctc gttgagcaga cgcgccagag ccaccgaagt cgcggtgttc ctgtcgtcga
      841 acgatcccgc acagccgacg aaccacagat actcggcggt gtccttcccg tcctgaccga
      901 acatcgggat ggggaagtcg agctcggagg tccagtcggc ccgggcctgc tggctcaagc
      961 cccatgggtt cgattggttc tccatcgcca cgaacgcctt tcccagctcc gaagggaatt
     1021 cagactccat cagggtcaga tagcggcgca tgtccaggat gcgatcgagg atgccgatgt
     1081 cgaccgggca gatctcgtca caggcccggc aagtggtgca ggcgaacacc tcctcggggc
     1141 gaatgcggtc gaacagcgat ccagccgcct cgaggaccat ggtcgtcgac accgggggcg
     1201 ccacgccggc taggttcgcc gccacctcgc ccgacttgag gaccatctct cgtgggtcga
     1261 gaggcttccc ggtgatgttg gcgggacaga ccgaggtgca ccggccgcag atggtgcagg
     1321 catcggtgtc gaagatctgc ttccaggtga ggtcggtggc cacactggca ccgatggtct
     1381 cgatgtcctc ggcttccatc aggttgggca tcggcctcat ggcgcctttt ggccgctcgt
     1441 tcaccgagag gaacatgttc accggcgagg tgaacatgtg cctcagcttc gtgagcggga
     1501 ggaccacgag gaagaccacg aacagcaccg cgtggctcac ccagaggaag agatgccaat
     1561 cgcccgccgt cgactgcgcg aagaggtacg agagcgggta cccgaggaac gaccagacct
     1621 cgaaatcggg ccggccggca agggagatgc gcgccgcctc gattagcaag ccggtgatcc
     1681 cgatcaatgc gagaaccgac aggatgagtg cgtcctccgg cctcgttttg gaccgaatcc
     1741 tccaggcagg caccacatat cggccgacga ctgccagagc cagccctccc aggaagacca
     1801 agccggccag atcgagcacc gccgagtatc cgaggtagaa gttgccatgg agaaacttca
     1861 ggttggccgg catgagatgg tctatctcga gggtcaccgt ccccaggaac agcacgatga
     1921 agccgaagta gatcatggag tgcatgaccc ccgcgcgcgg gtcgcgcatc agggtccgca
     1981 tcgtgagacc ttcgaccagc cgggccaccc gtcttcccag atgaccggtc cgccgatctt
     2041 cccctccctg ttgccaactg acggcgcgtt gcgcgaagag atggaaggtc agccagagaa
     2101 acccggccaa cccgacgtag aagacgagga cggccgcatc ggggacgttt ccgaacacct
     2161 ctcggcccac atcgaagtgc gggtgctccg gaagggttcc caggaggccc agc
//
LOCUS       DASUTA010000221         2139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_323158, whole genome shotgun sequence.
ACCESSION   DASUTA010000221 DASUTA010000000
VERSION     DASUTA010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323158"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VFZ80_04450"
     CDS             <1..380
                     /locus_tag="VFZ80_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012184686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NDP-hexose 4-ketoreductase"
                     /protein_id="HEX6286712.1"
                     /translation="RPEFINRLDEIIVFHELEMDELKQIVDLMLSRVRDQLANQQLDL
                     ALTDQAKEYLGTQGYDPELGARPLRRAIQRLLEDPLSERVLLGEFKAGTTIVVDFDKE
                     SDELTFDGISSPDSPPVELAGQE"
     gene            444..1100
                     /locus_tag="VFZ80_04455"
     CDS             444..1100
                     /locus_tag="VFZ80_04455"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX6286713.1"
                     /translation="MPKIFALLVVLVAGLGPGEEPEYVPAGTEAYSIDLEVDGFINGR
                     MESDRLLNVRGCLLERDAAYMYALMLEAAEEDGVYLGFEDCYRSYSQQKAAYERRCPI
                     VETPLYSVDASTGEKVQTGVKKQRECTGAPVAPAGASNHGWGRAVDFTDGRGVLTCYD
                     SEFDWLRNNAHRFGWVHPGWAACGLETQEPWHWEWAGVTDPTLVPFVSIDPTLVPTLE
                     "
     gene            complement(1239..>2139)
                     /locus_tag="VFZ80_04460"
     CDS             complement(1239..>2139)
                     /locus_tag="VFZ80_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6286714.1"
                     /translation="NWIRDVAPNFGLDLEEFLSQEAIEVWLRDPANQETIQNLLFGFG
                     AGAGVVLRGVAETIAVIALAPVLAIYILMDLNRFKSQSLELTPPQHRNEVAYVGGELG
                     TAMGSFVRGQLLVAVIVGIASSIGMWAIGLPFWLLIGIIAGFLNLIPFLGPVVGGALA
                     ALVALLNGDVWQALWAVVIMIGIQQVDNHLITPMVQRARVNLSPLVIVLALIIGGSLA
                     GLLGVLVAIPATAAIRIIVGHMWRTRVLGQSWEEASEAMIELTEPPERLARLSRKSPE
                     QSRLFDTQELSAVSEDVTEDEKV"
BASE COUNT          426 a          672 c          653 g          388 t
ORIGIN      
        1 tccgccccga gttcatcaac cggctcgacg agatcatcgt gttccacgag ctggagatgg
       61 acgagctcaa gcagatcgtc gatctcatgt tgtcgagagt ccgggatcag ctggcgaatc
      121 agcagctcga cctggccctg accgatcagg ccaaggagta cctggggaca cagggatacg
      181 atcccgagct cggggcgaga ccattgcgtc gtgccatcca gcgtctcttg gaggaccccc
      241 tgtccgaaag agtcctcctc ggcgagttca aggccgggac cacgatcgtg gtcgacttcg
      301 acaaggagag cgacgagctg acattcgacg gcatctcctc gccggattcc cctcctgtgg
      361 agctcgccgg acaggaatag tcgcgaaaac ctcctcaagg gggcggaggc gctcaccgat
      421 gtctattcag cctgagcgct cctatgccca agatctttgc ccttctggtc gtcctcgtcg
      481 ccggcctcgg gccgggggag gaacccgaat acgtccccgc cggaaccgag gcctattcaa
      541 tcgatctcga agtcgatgga ttcatcaacg gacgaatgga atcggaccgt ctcctcaacg
      601 tgaggggctg tctcctcgag cgtgacgcgg catatatgta cgccctgatg ctggaggcgg
      661 ccgaggagga tggcgtctac ctggggttcg aggactgcta ccgcagctac agccagcaaa
      721 aggcggccta tgagaggcgt tgtccgattg tcgagacccc tctctactcg gtagacgctt
      781 cgacggggga gaaggtccag accggggtca agaagcagcg ggagtgcacc ggtgcaccag
      841 tggcacctgc cggcgcatcg aaccacggtt ggggccgggc cgtcgacttc accgacggca
      901 ggggcgtcct cacttgctac gactcggagt tcgactggct gaggaacaac gctcaccgtt
      961 tcggatgggt tcacccggga tgggccgcgt gcggtttgga gacccaggaa ccgtggcatt
     1021 gggagtgggc cggggtcacc gacccgacac tcgtcccctt tgtcagcatc gaccccaccc
     1081 tggtccccac cctggagtag tgggtggctt attgcgacct ccggtagtta gagccgtatc
     1141 cggctccaac tacctccaga gtcagacccg atctctctct cgagctctct ctcgaggcac
     1201 ttcggctcgt atatggttct aactaccgga ggtcgggttc agaccttctc gtcctctgtc
     1261 acgtcctcgc tgacggcgct gagttcctgg gtgtcgaaga gacgtgactg ctcggggctc
     1321 ttgcggctga ggcgagccag cctctcgggg ggctcggtca gctcgatcat cgcctcgctt
     1381 gcctcttccc aggattgacc gagcactcga gttcgccaca tgtggccgac gatgatgcga
     1441 atggccgccg ttgccgggat tgccaccaat acgcccagga gcccggcgag ggaaccaccg
     1501 atgatcaggg caagcacgat gacgagaggt gagaggttga ccctcgccct ctgcaccatc
     1561 ggggtgatta gatggttgtc gacctgctga ataccgatca tgatcaccac cgcccacaac
     1621 gcctgccaga cgtctccgtt caaaagggcg acgagggcag cgagcgcacc gcccaccacc
     1681 ggtcccaaga acggaatcag gttcagaaag ccggcgatga tcccgatcag cagccagaaa
     1741 ggcaacccaa ttgcccacat gccaatactg gaggcgatgc cgacgattac agcgaccagc
     1801 agttgcccgc gcacgaaaga gcccatggcg gtgcccagct cgcccccgac ataagccacc
     1861 tcgtttctgt gttggggcgg ggtcagctcc agagactggg acttgaaccg gttgaggtcc
     1921 atcaggatgt agatggccag cacaggagcc agcgctatga cagcgatcgt ctcggcgaca
     1981 ccacgaagca ccacacctgc gccggcgccg aacccgaaca gcaggttctg aatcgtctct
     2041 tgattggcgg ggtcgcgcaa ccacacctcg atcgcctcct gagagaggaa ctcctccaga
     2101 tcgagcccga agttgggggc gacatccctt atccagttg
//
LOCUS       DASUTA010000222         7799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1099881, whole genome shotgun sequence.
ACCESSION   DASUTA010000222 DASUTA010000000
VERSION     DASUTA010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099881"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFZ80_04465"
     CDS             <1..220
                     /locus_tag="VFZ80_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006075005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286715.1"
                     /translation="EIIAPSSGPIEVELPVERYRMHLIDEDVALVRYVSNETLDGGSR
                     STERTSIWVNTNEGWQLRFHQGTPLPDV"
     gene            381..1271
                     /locus_tag="VFZ80_04470"
     CDS             381..1271
                     /locus_tag="VFZ80_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HEX6286716.1"
                     /translation="METSQPVPGSEYPVYLLHVETWDGLYAPIGLRLPEGPGPFPTVV
                     MASGNGGGGMAWIRDAVNNRAYTMERLLEAGYACAWIRYRSEVELGYHNGGPLVRDHR
                     QGGDMFNRAPLEYEDEIAIIEYLKRRPDVDGDRVAHIGSSHGGEMALKITSEYHGLAA
                     AVANEPAAHEFLALTPDETAPMNEETGLRNIENMQMTEVEKVRSRIDTALASERIAGI
                     ETPILVIGRETDHLQGIFRTTFELLEEAGKDVEWVSYDHPIHGFVFPFRGDDGEYDVD
                     AMQDEAITAMIAFLRRHLGS"
     gene            complement(1272..2114)
                     /locus_tag="VFZ80_04475"
     CDS             complement(1272..2114)
                     /locus_tag="VFZ80_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6286717.1"
                     /translation="MARYGIKTPNHHTTWDNMLEVWREADGIELFESAWNWDHLYPLR
                     SDPHGLSLDGWVTLAALARETSRIRIGSMVNGMHFRHPSITAKMAVTLDHVSAGRAYL
                     GLGAGWFEEEAKAHGIELGSLRERFDRFDEGLQVIVSLLTNEETDFSGDFYELKGALS
                     EPKATQQPHPPIVIGGTGEKRTLRAVARWAQMWDALLVDTDEWTRLRDVLEDHCREAE
                     RDSSEIDCSVHVRYSKEDDVAEVAEQAARHLDNGVDVVIFTMGSPNDASMVSALARAL
                     EPVG"
     gene            complement(2124..3713)
                     /locus_tag="VFZ80_04480"
     CDS             complement(2124..3713)
                     /locus_tag="VFZ80_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6286718.1"
                     /translation="MPEDQYDVVVAGAGHNSLVSAAYLAKAGLRCLVLEARSTIGGNT
                     ATEELTLPGYHHDSCSTAHNIIQASPTLRFNELGLGDFGLEYIQPDPVVHIPFPDGTS
                     VTMWRDIDQTVAELERFSRSDGAAYRRMMEEYDRVKGVFADSRYTPFGFGPTVDQRLT
                     EHPEGAMWRKLIASSAWDIISREFEHPNSRAFMLWLAFMTVQPPQRVGTGRLAYSLAY
                     GRQTSSWIIPKGGSAALPLALSRVIEANGGTILAGRRVDRLIVENDRCVGVVDEIGEE
                     HRASKAVLSTIHIKHLLDMAPKERWGSDFVTGVDEWRAGVALCPAHYATTEAPAFDTP
                     EGTIRPLASGVPHSVERMLRIEHDFLTGSLALDDPPLLVLCPTVADPTRAPDGNHTLK
                     VIGFHPYELADGAEKWDEIKTDVATAHMDHLRRYSPNLTDETILASAVKSPLDLERMN
                     AHNWHGSCHGGDQDMAQSGAFRPVYGWAQHRMPIAGLYQTGATTYPGPSVSAGPGRNA
                     AWVMLDDLGIDRGKVFADTRG"
     gene            3789..5507
                     /locus_tag="VFZ80_04485"
     CDS             3789..5507
                     /locus_tag="VFZ80_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX6286719.1"
                     /translation="MAEGIHIDWDVPIEMDDGLVVRADVYRLEDDGQYPVILSYGPYA
                     KGLAFQEGYPDQWRLMTNQHPDVATGSTNRYQNWEVVDPEKWVPDGYVVVRVDSRGCG
                     RSPGYIDVFSPRETDDLVACIEWAGTQPWSTGKVGLNGISYYAINQWHAASRQPQHLV
                     AMCVWEGAADFYRDMTHHGGILSTFWANWYDMQVKTVQHGLGSRGPVSRVTGVPVTGD
                     HDLDEGRLEANRADLGAQVRDHPLDDDFHRDRSPVWPNVRVPLLSAGNWGGQGLHLRG
                     NVEGFVRSASSQKWLEIHGREHWTHFYTDYGVGLQKRFFARFLKDEDNGWDSEPPVLL
                     QIRHLDRFEERKAESWPLPETRWTTLFLDAVARSLDTDAPGSDTALSYDATGHGVTFL
                     MDPFDTETELTGPLSLKLFVSSSTEDADIFAVVRVFDPRGDEVTFQGAIDPHTPIAQG
                     WLRASHRRLDPGLSLPYRPYHVHQEREPLVPGETYGLSVEIWPTSVVIPAGYRLGLSI
                     RGNDYEYEGDLGEASLNTFKNRLTGCGPFLHNDPVDRPDEVYGGAVTIHTGPGHPSSL
                     LVPVID"
     gene            complement(5520..6542)
                     /locus_tag="VFZ80_04490"
     CDS             complement(5520..6542)
                     /locus_tag="VFZ80_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6286720.1"
                     /translation="MAVVEKRADIPERAADRRPGSRSRRVTLILSMAFVLLFPLIAYP
                     IFDSYAYVMQLGMLTLMWIAMSSSWNILGGFAGYISLGHGLFYAIGGYSAGLALAHWD
                     ISPFLFLPVAGLAAMAVGFLAGLITLRTRGPSFIVATIAMLFLGLLAFDNWNFIGASS
                     GLDLPLFQFPIEFLKVPFYYAMAVTAMFAVYLSYRVAHSKFGLGLRAISQDETKAEMA
                     GINTRWYKVWAFALSAFAIGMAGAIYGYSLTHLRPLTFFAIAVAARMVLMAIIGGRGT
                     VAGPVIGAILILGVNEVAVRQMGESALNIVFTGLILLGALLFFPEGIVGSLRKAGRLP
                     AFLDWD"
     gene            complement(6546..7418)
                     /locus_tag="VFZ80_04495"
     CDS             complement(6546..7418)
                     /locus_tag="VFZ80_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6286721.1"
                     /translation="MRWVQVLVLGLLLGGVYAMMASGLTLLFGVMRVVNLAHAAFMLV
                     ASYLTYVLFESFAVDPMISLIITMPLMFGLGVALYLLLFPRLEDNPRYVEMTVLLTFG
                     LALIVEGALTAIFTGIFRSVRPEYATTSINIGDLFIPTSQFYASMISLGLLILLWVVL
                     RFTKTGTAVRATMQNRAAAQTVGVNVRRVSAIAFGMGMALAGAAGSLLSFMFPFFPAR
                     HWQWVALLLALIVLGGMGSIKGAVIGAMVLAVASAFASTIIGPTWSPITFYLTLFLIL
                     MLRPEGLFGAKVRL"
     gene            complement(7423..>7799)
                     /locus_tag="VFZ80_04500"
     CDS             complement(7423..>7799)
                     /locus_tag="VFZ80_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6286722.1"
                     /translation="VYEAFPILAERRAQHAGNLSGGEQQMVTLGRALMIEPKIVMIDE
                     PSLGLAPMIADQIFEKIRGFSELGMTVLLVEQNAAKGLDAVDWGFVLDLGSLEFEGPA
                     DGILGDPRIRDLYLGKAMSRKR"
BASE COUNT         1529 a         2442 c         2477 g         1351 t
ORIGIN      
        1 ggagatcatc gcaccatcga gcgggcccat cgaggtggag ttgcccgtgg agagataccg
       61 catgcacctg atcgacgagg acgttgccct cgtccgatac gtcagcaacg agactctcga
      121 cggcggatcg cggtccaccg agcgcacgtc gatctgggtg aacacgaatg aaggctggca
      181 actcaggttt caccagggca ccccgttgcc cgacgtctga gaggtcgggt cgaaaagctc
      241 aggtccgccg ttccagaccc cccgggacaa cgggcttcaa cttctcgagc tggcaccaat
      301 taagctgccc gccttcgacg atcggagcca ctgtcgcgtc tctcccaaaa aaatcgctcg
      361 agggcatcgg tcacccagcg gtggagacat cgcagccggt acccggctcc gagtatccgg
      421 tctacctcct ccatgtagag acctgggatg gtctctatgc ccccataggg cttcgtctgc
      481 ccgaggggcc cggccctttc ccgacggttg tcatggcctc gggcaacggc ggagggggga
      541 tggcgtggat cagagacgcc gtgaacaacc gggcctacac gatggagagg cttctcgaag
      601 ctggatatgc gtgtgcctgg atccgatatc gcagcgaggt ggagctgggc tatcacaacg
      661 gcgggccgct ggtcagggac catcgtcagg gtggggacat gttcaaccgg gcgccgctcg
      721 agtacgaaga cgagatcgcc atcatcgagt acctgaagag acggcccgac gtcgacgggg
      781 atcgtgtggc ccacatcggc agcagccacg gtggggagat ggccctgaag atcacctccg
      841 agtatcacgg tctggccgcg gcggttgcca acgagcccgc agcccacgag ttcctcgctc
      901 tcacccccga cgaaaccgct ccaatgaacg aggagaccgg acttcgcaac atcgagaaca
      961 tgcagatgac cgaggtggag aaggtgcgct cccgaatcga taccgccctg gcgagcgagc
     1021 ggatcgccgg gatcgagact ccgatactcg tgatcgggag ggagaccgac catctccagg
     1081 gcatctttcg caccaccttt gagctactgg aggaagcggg aaaggacgtg gagtgggttt
     1141 cctatgacca tcccatccat ggcttcgtct tccccttccg gggagatgac ggtgaatacg
     1201 acgtcgacgc gatgcaggat gaagccatca ccgcgatgat cgccttcctg cgtcgccacc
     1261 tcggaagttg atcagccaac cggttcgagc gctcgggcga gcgcagaaac catggaggcg
     1321 tcgttggggc ttcccatggt gaagatgacc acgtcgacac cgttgtcgag atgtcgggct
     1381 gcctgctcgg cgacttcggc gacatcgtcc tccttggagt agcgcacgtg gaccgagcaa
     1441 tcgatctccg atgagtcgcg ttccgcctcg cgacaatggt cctcgagcac gtcacggagc
     1501 cgggtccact catcggtgtc gaccaacaag gcatcccaca tttgcgccca acgggcaacg
     1561 gcgcgcagcg tccgcttctc acccgttcct ccgatgacga tcggcgggtg aggctgctgg
     1621 gtcgccttcg gctcgctgag ggcacccttc aactcgtaga agtcaccgga gaagtcggtc
     1681 tcctcgttgg tcagcagact cacgatcacc tggagacctt cgtcgaagcg gtcgaaccgc
     1741 tcccggagag agccgagctc gatgccgtgg gccttggcct cctcttcgaa ccatcccgcc
     1801 cccagcccca ggtaagccct gccggcagag acgtgatcca gcgtcacagc catcttggcg
     1861 gtgatcgacg ggtgccggaa atgcatcccg ttgaccatgg agccgatccg aatccggctc
     1921 gtctcccgtg ccaatgccgc cagtgtcacc cagccgtcga gcgagaggcc gtgagggtcg
     1981 ctgcggaggg gatagagatg gtcccagttc cacgccgact cgaacaactc gataccgtcg
     2041 gcctcccgcc agacctccaa catgttgtcc caggtcgtgt ggtggttcgg tgtcttgatc
     2101 ccgtatctgg ccattcgatc cccctagccg cgcgtgtcgg cgaacacttt gccccgatcg
     2161 ataccgaggt cgtccagcat cacccaggcg gcgttccggc cggggcctgc cgacaccgaa
     2221 ggccccggat aggtggtggc accggtctga tagagcccgg ctatgggcat ccggtgctgt
     2281 gcccacccgt acacaggccg gaaggcgcct gactgagcca tgtcctggtc ccctccatgg
     2341 caggaaccgt gccaattgtg cgcgttcatc cgctccaggt cgagcgggct cttcactgcg
     2401 ctcgccagga tcgtctcgtc ggtcagattg ggcgagtagc ggcgcaggtg gtccatgtgc
     2461 gcagtagcca cgtcagtttt gatctcgtcc cacttttcag ctccgtccgc cagctcatag
     2521 ggatgaaagc cgatcacctt caatgtgtga ttgccgtctg gggcccgggt ggggtcggcc
     2581 accgtgggac agagcaccaa gagcgggggg tcgtccaggg cgagcgatcc cgtgaggaag
     2641 tcgtgctcga tccgcagcat ccgctcgacg gagtgcggca cccccgaggc cagggggcgg
     2701 atagtccctt ccggggtatc gaacgccggg gcctcggtgg ttgcgtaatg ggcaggacag
     2761 agggcgaccc cggcacgcca ctcgtccact cctgtgacga aatcactacc ccatctctcc
     2821 tttggcgcca tgtccaacag gtgcttgatg tggatggtgg atagcaccgc cttcgaggcg
     2881 cgatgctcct caccgatctc atcgacaact ccgacgcagc ggtcgttctc cacgatgaga
     2941 cgatcgacgc ggcgcccggc gaggatcgtc cctccgttgg cctcgatgac ccggctcagc
     3001 gccagcggca aagccgccga gccacccttg gggattatcc aagagctggt ctggcgtccg
     3061 tacgccagcg agtaggcgag acggccggta ccgacccgct gcgggggctg gacggtcatg
     3121 aacgccagcc agagcatgaa ggcgcggctg ttgggatgtt cgaactccct gctgatgatg
     3181 tcccaggcgc tggaggcgat gagcttcctc cacatcgcac cctccggatg ctcggtcaac
     3241 cgctggtcca cggtggggcc gaagccaaac ggtgtgtacc tgctgtcggc gaagactccc
     3301 ttcacccggt cgtactcctc catcatgcgc cggtaggcgg ctccgtccga cctggagaat
     3361 cgttcgagct cggcgacggt ctggtcgatg tcccgccaca tcgtcaccga cgtaccgtcc
     3421 ggaaacggga tgtgcaccac ggggtcgggt tggatgtact cgaggccgaa gtcgccgagg
     3481 ccgagctcgt tgaagcggag cgtggggctg gcctggatga tgttgtgggc ggtggaacag
     3541 ctgtcgtggt ggtagccggg gagggtgagc tcctcggtgg cagtgttccc tccgatggtc
     3601 gatctcgcct ccaatacgag gcaacgaaga ccggccttgg cgagataggc cgccgacacc
     3661 aggctgttgt ggccggcgcc tgccacgacc acgtcgtatt gatcctcagg cattctcatc
     3721 gactcctcgg gcacggcagt ttctgcaaac gattgtcagg accataatcg cctagcctgc
     3781 gggctcgaat ggccgaggga atacacatcg attgggacgt ccccatcgag atggacgacg
     3841 gtctcgtcgt gcgcgccgac gtgtatcggc tcgaggatga tgggcaatac ccggtcatcc
     3901 tgtcgtatgg gccgtatgcg aaaggccttg ccttccagga gggttatccc gaccagtggc
     3961 ggctgatgac caaccagcac cccgacgtgg cgacgggatc cacgaaccgc tatcagaact
     4021 gggaggtggt cgatccggag aagtgggtgc ccgacggcta cgtggtggtc cgggtcgact
     4081 cgagaggttg tggacgctca ccgggataca tcgatgtttt ctccccacgg gagaccgatg
     4141 acctggtggc gtgtatcgaa tgggcgggga cccaaccatg gagcaccggc aaggttggcc
     4201 tcaacggaat ctcgtattac gccatcaacc aatggcatgc cgcctcgcga cagccacagc
     4261 atctcgtcgc catgtgcgtc tgggagggag cggccgactt ctaccgcgac atgacacacc
     4321 atggcgggat cctctccact ttctgggcca actggtacga catgcaggtc aagaccgtcc
     4381 agcacggcct ggggagccgg ggaccggtga gccgggtgac gggcgttcct gtgaccggag
     4441 accacgatct cgacgagggc cgattggagg ccaaccgggc cgatctgggc gcccaagtgc
     4501 gcgaccaccc cctcgacgac gatttccatc gcgatcggtc cccggtgtgg cccaacgtca
     4561 gggtgccgtt gctctcggca gggaattggg ggggccaagg tctgcatctc cgcgggaacg
     4621 tggagggttt cgtgagatcg gccagctcac agaagtggtt ggagatccac ggtcgggaac
     4681 attggaccca cttctacacc gactatgggg tcggtctcca gaagcggttc ttcgcccggt
     4741 tcctcaaaga cgaggacaat ggctgggaca gcgaaccgcc agtcctgctg caaattcggc
     4801 acctcgaccg tttcgaggag cgcaaagccg aatcgtggcc gctgccggag acccggtgga
     4861 cgaccctctt tctcgacgcc gtggccaggt cgctcgacac agacgcacct ggatccgata
     4921 cagccctgtc atacgacgca accggacatg gcgtgacctt tctcatggac cctttcgaca
     4981 ccgagaccga gctcaccggt ccgttgtcac tgaagttgtt cgtatcgtcg tcgaccgagg
     5041 atgccgacat ctttgccgtg gtcagggtgt tcgacccccg tggcgacgaa gtgaccttcc
     5101 aaggtgcgat cgatccccac actccgatcg cccagggatg gttgcgcgcc tcccaccgcc
     5161 gtctcgaccc cgggttgagc cttccctacc ggccctatca cgtccaccag gagcgggagc
     5221 cgctggtccc gggggagacc tacgggttga gcgtcgagat ctggccgacg tcggtggtca
     5281 tcccggccgg ctaccgcctg ggtctcagta tccgtggcaa cgactacgaa tacgaagggg
     5341 acctcgggga ggcgtcattg aacacgttca agaatcgcct caccggatgc ggcccattcc
     5401 tccacaacga tcctgtcgac cgcccagacg aggtgtatgg cggtgcagtg acgatccaca
     5461 ctggccccgg ccacccgtcc tctcttctgg tcccggtgat cgattaggcc gactcgaagt
     5521 cagtcccagt cgaggaacgc ggggagccgg cccgccttgc gcaggctgcc gacgatcccc
     5581 tcggggaaga agaggagggc tcccagcagg atcaacccgg tgaagacgat gttgagagct
     5641 gactccccca tctggcggac agcgacctcg ttgactccca ggatcaggat ggcaccgatc
     5701 acgggccctg ccaccgtgcc ccgccccccg atgatcgcca tcaacaccat gcgggccgcc
     5761 accgcgatgg cgaagaaggt gagtgggcgg aggtgggtca atgagtatcc gtagatggct
     5821 ccggccatgc cgatggcgaa agcgctcagc gcaaaagccc acaccttgta ccagcgggtg
     5881 ttgatcccgg ccatctcggc cttggtctca tcctggctga tggctcgcag gcccagaccg
     5941 aacttggagt gagccacccg gtacgacagg tacacagcga acatcgccgt gaccgccatt
     6001 gcgtaataga aggggacctt caagaactcg atcgggaact ggaagagggg caggtccaga
     6061 ccactcgacg caccgatgaa gttccagttg tcaaaagcga gcaggcccag gaagagcatg
     6121 gcgatggtgg ccacgatgaa cgaagggccc cgcgtcctca gagtgatgag ccctgccagg
     6181 aaacccacag ccatggcggc gagccctgca accggtaaga acaggaacgg ggagatgtcc
     6241 caatgggcca gtgccaggcc ggcgctgtag ccaccgatgg cgtagaagag gccgtgaccc
     6301 agcgagatgt acccggcgaa gccgccgagg atgttccagc tcgacgacat tgcgatccac
     6361 atcagggtca gcatcccaag ctgcatgacg taggcgtagg agtcgaagat gggataggcg
     6421 atcagcggga agagcaagac gaaagccata gagaggatca gggtcacccg gcgagatcgc
     6481 gagcccggcc tgcgatccgc cgcccgctcc gggatgtctg ctcgcttttc gacgactgcc
     6541 atttcctaca acctcacctt ggcgccgaac agaccctcgg gtctcagcat caggatcagg
     6601 aacagagtga gatagaaggt tatgggtgac caagtgggcc cgatgatggt gctggcgaac
     6661 gcgctggcga ctgccaggac catcgcaccg atcacggctc ccttgatgct ccccatccca
     6721 ccgagaacga taagggcgag aagcagcgcc acccactgcc agtgtctggc cgggaagaaa
     6781 gggaacatga agctcaacaa cgacccggcg gctccggcca gcgccatgcc catgccgaat
     6841 gcaatcgcgg acacccgccg gacgttcacc cccaccgtct gtgccgcggc ccggttctgc
     6901 atggtggccc tgacggcggt gcctgtcttg gtgaaccgga gcactaccca gaggaggatg
     6961 aggaggccaa gactgatcat cgaggcgtag aactggctgg tgggtatgaa gaggtcaccg
     7021 atgttgatag atgtcgtggc gtactcgggc cgaacgctgc ggaatatgcc ggtgaagatg
     7081 gcggtgagcg cgccttcgac aatgagggcc agaccgaagg tgagcaacac tgtcatctcc
     7141 acgtaacggg ggttgtcttc gagacgtggg aacagcagca ggtacagcgc taccccgagc
     7201 ccgaacatca ggggcatggt gataatcaaa gagatcatcg gatccactgc gaagctctcg
     7261 aacaggacgt aggtgaggta cgaagcgacg agcatgaacg cggcgtgagc cagattgacg
     7321 acgcgcatga ccccgaacag cagagtgagc ccggaggcca tcatggcgta cacgccaccg
     7381 agcaagaggc cgaggaccaa tacctgcacc caccgcatca gctcacctct tcctggacat
     7441 tgccttgccc agatagaggt cgcgaatcct cgggtcgccg aggatcccgt ctgctgggcc
     7501 ttcgaattcg agtgatccga ggtcgagcac gaaaccccag tcgactgcat cgaggccctt
     7561 ggccgcgttc tgctcaacaa gcagcaccgt catgccgagc tcgctgaatc ctctgatctt
     7621 ctcaaagatc tgatcggcga tcattggggc caggccgaga ctgggctcgt cgatcatcac
     7681 gatcttgggc tcgatcatca acgccctgcc cagggtgacc atctgttgct cgccaccgga
     7741 gagattgccg gcgtgctggg ccctgcgctc ggcaaggatc ggaaaggcct cgtagaccg
//
LOCUS       DASUTA010000223         2529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1747612, whole genome shotgun sequence.
ACCESSION   DASUTA010000223 DASUTA010000000
VERSION     DASUTA010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747612"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFZ80_04505"
     CDS             <1..202
                     /locus_tag="VFZ80_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286723.1"
                     /translation="KKTADSVRAWADDLERSDRSRVRTYVGLGLLGALMAVVAYLMTP
                     KGKKHKERLQELGNEVVERVAS"
     gene            complement(619..1386)
                     /locus_tag="VFZ80_04510"
     CDS             complement(619..1386)
                     /locus_tag="VFZ80_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6286724.1"
                     /translation="MSQIESLLESEYQQLYRFALSITRDPSHAEDLTHDTLIRAIERE
                     DQFSGDTSKLGAWLRRILHNHAVDTARRSARELAVDEVEDRWKDDDYTVNPEVFALKA
                     QDTYELEDALARIPFIYRSTVLLHDVEGWTARQIAELQDIGLPAAKQRLRRGRMALVT
                     ALGEGAERRHLLKGIPMRCWDARQHISDYLDGSLDKSTAQIVEAHLETCPTCPPLYAA
                     LVDAHRNIHSLRDPDSVIPPDLDRRLRRLLSKRAQGS"
     gene            complement(1383..1961)
                     /locus_tag="VFZ80_04515"
     CDS             complement(1383..1961)
                     /locus_tag="VFZ80_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX6286725.1"
                     /translation="MVSPIRPFPVPHQHRNRASEGRLTDSRPQRGGPVVSPRVPNEKA
                     ETQLMLKPTLVEVTAPWCGECHAMRPTIERVAGRYPDVAFRVIDASVDHEMVGSLGVK
                     GTPTLIGCARGEEIFRFTGRRTSAELEAMFDAVSGARPIATVGRGDVLVRIGAGVALI
                     VSGLLSGPVWVLVGLGSAVGLFGLLTVRNRSR"
     gene            complement(1892..2116)
                     /locus_tag="VFZ80_04520"
     CDS             complement(1892..2116)
                     /locus_tag="VFZ80_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEX6286726.1"
                     /translation="MSFANEAAWDRIARVVLGIVLLYLGWAEVVTGGWGLVLKVIGFV
                     PLLTGLVGWCPLYALFRFHTNTETGRAKAV"
     gene            2240..>2529
                     /locus_tag="VFZ80_04525"
     CDS             2240..>2529
                     /locus_tag="VFZ80_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286727.1"
                     /translation="MSALKFPGTTEFSRRALLLEYATIAWNVGEAVFTMILGSVAGSL
                     ALISFGTVSIVEVFASSVVVWHVRSGNDGDQHHRTSLALRLLAGAFAVLAVAL"
BASE COUNT          492 a          736 c          784 g          517 t
ORIGIN      
        1 caaaaagacc gccgactcgg tccgagcttg ggccgacgat ctggagcgat ccgatcgatc
       61 tcgcgtgcga acctacgtcg gtctcggcct cctgggcgcg ctcatggcgg tagtcgccta
      121 tttgatgacg ccgaaaggca aaaagcacaa ggagcggctc caggagctcg gcaacgaagt
      181 ggtcgaaagg gtcgccagct gaggatttcg cggtcgcggt cagggtatta gaggctggcc
      241 ctcgtcagcg gcgtgaacgg atcgtgtcac gcggtcgtaa tgcaacggag aagggacgaa
      301 tcgaagaaga gaatcacagt tgcggcagtc gctggatgct tgatgttgtt cggtgtcact
      361 cctgctgcct ccgcccacgt tcgtgcgttc actccgctcg gttgcctcac cgccgacaat
      421 gcgaactccg gggcgctgtc gggtttgtga aggctgccga cgctgccact ccgctgaacg
      481 gtgccactca gataatcccc tggaatgccg gcgccaagct gcgtctggcg gccagggtgc
      541 ggccaacgcc tgctgacctg aattgatatc ggtctcgtca accacctgtg attgatgggc
      601 ttccgagcga gcagacggct aagagccctg tgctcgtttg ctgagaagtc gacgtagacg
      661 tcggtcgaga tcggggggta tcaccgagtc ggggtctctc aacgaatgga tgttccggtg
      721 ggcatccacc aaagctgcgt ataggggtgg gcatgtcggg caggtttcga ggtgggcctc
      781 gacgatctga gctgtggatt tgtcgaggct gccgtcgaga tagtcggaga tgtgttggcg
      841 ggcgtcccag cagcgcatgg ggattccttt caagaggtga cggcgttctg cgccttcgcc
      901 caaggcggtg accagggcca tgcgcccgcg cctgagtcgc tgtttggctg ccggcaagcc
      961 gatatcttga agctcagcga tttggcgggc tgtccatcct tcgacgtcgt ggaggaggac
     1021 tgtggaccgg tagatgaacg ggattctggc caaagcgtcc tcgagttcgt aggtgtcctg
     1081 cgctttcagg gcgaatactt cggggttgac cgtgtaatcg tcgtccttcc accgatcttc
     1141 gacttcgtcg actgcgagct cacgtgccga tcgacgagcg gtgtcgactg cgtggttgtg
     1201 gaggatgcgg cgcaaccatg ccccgagctt ggaggtatcg ccgctgaact ggtcttctcg
     1261 ttcgattgct cggatcagag tgtcgtgtgt caggtcctcg gcatgactcg ggtctctcgt
     1321 tatggatagg gcgaatcggt agagctgctg gtactcactc tcgagcagag actcgatctg
     1381 gctcacctgg agcggttcct gacggtgagg agaccgaaca gaccgaccgc agagccgaga
     1441 ccaaccagaa cccagactgg gccagacaac agacccgaca cgatgagggc gaccccggct
     1501 ccgatccgaa caaggacgtc tcccctacca accgtagcaa tggggcgggc tccgctcacg
     1561 gcgtcgaaca tcgcttccaa ttcggcgctg gtgcgtcggc cggtgaatcg gaagatttcc
     1621 tcgcctctgg cacaaccgat cagagtcggt gtgcccttga ctccgagact tcccaccatt
     1681 tcatgatcga cggaggcatc gatgaccctg aaggcaacgt cgggataccg gccggcgacc
     1741 ctctcgatgg tcggtctcat cgcgtggcat tcaccacacc agggtgccgt cacctcgacc
     1801 aatgttggct tgagcatgag ttgggtctcc gccttctcat tcggaactcg aggggagacc
     1861 acagggcctc ccctctgcgg acgtgaatcg gtcagacggc cttcgctcgc ccggtttcgg
     1921 tgttggtgtg gaaccggaaa agggcgtata ggggacacca tcccaccaac ccggtgagaa
     1981 ggggaacgaa accgatcacc ttcaagacca gaccccaccc cccggtgacg acttcggccc
     2041 agccgaggta gagaaggact atgcccaata cgactcttgc gattcgatcc caagcggcct
     2101 cgtttgcgaa actcatggtg tgctcctttc acttcgaatt gcacacccaa gagacgaacg
     2161 gacacagaaa ggggatacat cacctgttca accggtttcc cagaagccat tcggaggacg
     2221 atgatctgat cgaacattca tgagcgctct gaagttcccg ggcaccaccg agttcagccg
     2281 acgcgctctt ctcctcgaat acgccacgat tgcatggaac gtcggcgaag ccgttttcac
     2341 catgattctc ggctcggtcg ccggatcgct ggcactgatc agtttcggca cggtctcgat
     2401 cgtcgaagtc ttcgcatcct cggtcgttgt atggcatgtc agatcgggga atgacggaga
     2461 ccaacatcat cgaacgagtc tggccttgcg actactcgca ggagcattcg cagtactcgc
     2521 ggtcgccct
//
LOCUS       DASUTA010000224         2471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_711874, whole genome shotgun sequence.
ACCESSION   DASUTA010000224 DASUTA010000000
VERSION     DASUTA010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711874"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..712)
                     /locus_tag="VFZ80_04530"
     CDS             complement(179..712)
                     /locus_tag="VFZ80_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286728.1"
                     /translation="MRGVLTLVWTLLAGVAFIIWDCSWPIVDDPLALCPNVAGMGLPE
                     PLWVAWAFLSVSLVGLLAAWSSYLEDRHRRKTARPELALLANIGRLPDPYGTFQPDHP
                     ESDRVGELRRGVEAVEAAFSMDSTATRAMTGEWMKLLLQANTMHNEGTLETEDFKDLN
                     TRLLEAVVGPPSHRVPG"
     gene            complement(765..1856)
                     /locus_tag="VFZ80_04535"
     CDS             complement(765..1856)
                     /locus_tag="VFZ80_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020405461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HEX6286729.1"
                     /translation="MTRALRLCIVSVAMLAACSSGGAVESSTTTPRPGTTITTEAPTA
                     TTTSVTSTTSSAPSTTTTTLDAESLEIEAFPVPSGSRPHDVAPAVDGGVWYTAQSLGE
                     LGWLDPATGEIRHIPLGSGSRPHGVIVDDRGTPWVTDGGLNAIVSVDPANDEVTVYPL
                     PAEAPGANLNTAVFDDSGVLWFTGQEGIYGWLDPATGGMEVEPAPEGPGPYGITHTPS
                     GTVFYASLAGSYVGEITAGDVIVREPPTVDQGARRVWSDSMSRVWVSEWNSGQVSRYD
                     PETGEWAIWELPGSEAATYAVYVDDRDIVWLSDFGGNAIVRFDPSTETFTRYPLPHNP
                     GNVRQILGRPGEVWGAESAADHLILIRTR"
     gene            1913..2095
                     /locus_tag="VFZ80_04540"
     CDS             1913..2095
                     /locus_tag="VFZ80_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286730.1"
                     /translation="MRGLFVLAVVLLIVAVIVAVVGPSMPAGSPLRAAGDGIRGIGDW
                     LGSGFGGGYGEIAPGG"
     gene            2134..>2471
                     /locus_tag="VFZ80_04545"
     CDS             2134..>2471
                     /locus_tag="VFZ80_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286731.1"
                     /translation="MLQVLIDVLVPVFAVVAIGFGLSRWIGIEPRALATLAYWVLGPA
                     FIFDVLATAELDPDVVVKVVATSAAVMVVAGAVAAVVMRVIGSSFSELAATVLTSIYG
                     NAGNFGLAISA"
BASE COUNT          442 a          792 c          797 g          440 t
ORIGIN      
        1 gaggagttgc gtgccatcca cgggcgcctg agagccgacg acgtcgacgt cgacaacctg
       61 atcaaggatg tcgagcgcgc ctcggagctc ctcgacttct gccaaagacg gatcaccgcg
      121 gtcggcgagc agctcgaaga ggttctcacc gacttcgaaa gaccggccga ctgagagctc
      181 agccagggac gcggtgcgac ggggggccga ccactgcttc gagcaaccgg gtattgaggt
      241 ctttgaaatc ctcggtctcg agcgttcctt cgttgtgcat ggtgtttgcc tgcaggagca
      301 acttcatcca ctcacctgtc atcgctcgag tcgccgtgga atccattgag aaggcggctt
      361 cgacggcctc gacgcctcgt ctcagctcac ccacccggtc actctcggga tgatccggtt
      421 ggaacgtgcc gtagggatcg ggcagacgcc cgatattggc gaggagggca agctccggtc
      481 gagcggtctt ccgacggtgt ctgtcctcaa gataggagct ccaggcggcg agcagaccca
      541 ccagcgacac actgagaaac gcccacgcca cccaaagagg ctccggcagt cccatcccag
      601 ccacattggg gcacaacgcg agagggtcgt cgacgatcgg ccacgagcag tcccaaatga
      661 tgaacgccac ccccgccaga agcgtccaaa ccagggtcaa gacccctctc atccacatga
      721 gatcacatcg gccccagagc gccgtttctt gactcgtgcc ggccctaacg agtcctgatc
      781 aatatcagat gatcggccgc cgattcggcg ccccaaacct cacctgggcg acccaggatc
      841 tggcgaacgt tccctgggtt gtgaggcagt ggataccgag tgaaggtctc ggtcgaggga
      901 tcgaatcgga cgatggcatt ccccccgaag tcgctgagcc aaacgatgtc ccgatcatcg
      961 acgtagacgg cgtacgtcgc cgcctcgctg cccggaagct cccagatcgc ccactcgccg
     1021 gtctccgggt cataccgact gacctgaccg ctgttccact cgctgaccca cactcgggac
     1081 atcgagtccg accagactcg gcgggcgccc tggtccacgg tgggaggctc gcggacgatc
     1141 acatctccgg cggtgatctc gccgacatag gagccggcta gagacgcata gaaaaccgtc
     1201 ccgctcgggg tatgggtgat gccatagggc cccggtcctt ccggagccgg ctcgacctcc
     1261 atcccaccag tcgccgggtc gagccacccg tagatcccct cctgtcccgt gaaccagaga
     1321 acaccactgt cgtcgaagac agccgtattc aggtttgcgc ccggagcttc ggcagggagt
     1381 ggatagaccg tcacttcgtc gttcgcgggg tcgacggaca cgatggcgtt cagcccgccg
     1441 tcggtgaccc aaggcgtgcc ccgatcatcg acgatgacgc catgtggacg agagccggag
     1501 cccaagggga tgtgccggat ctcgccggtc gccgggtcga gccagccgag ctcgccgaga
     1561 ctctgggcgg tgtaccagac cccgccatcg accgccggag ccacgtcgtg aggacgcgat
     1621 ccggaaggta cggggaatgc ttcgatctcg agactctcgg cgtcgagggt cgtcgttgtc
     1681 gtcgagggtg cactgctggt ggtggacgtg acacttgtcg tggtggcagt cggcgcctcg
     1741 gtcgtgatcg ttgtccccgg gcgaggagtg gtcgttgatg actcgaccgc accacctgac
     1801 gagcacgccg cgagcatcgc gacggacacg atgcagaggc gaagggccct ggtcaccaac
     1861 aaagggtaag ggcagagccc gaataggcgt cgccgggtag cttgtgaaca cgatgcgagg
     1921 tctcttcgtg ctcgccgtgg tgctgctgat agtggcagta atcgtcgccg tggtcggacc
     1981 ctcgatgcct gccggcagcc cgctccgcgc cgcaggggac ggcattagag gcatcggcga
     2041 ctggctgggc agcggatttg gcggggggta cggcgagatc gcaccgggcg gatgagcttc
     2101 ggcggcaatc accacccgtc ctaacgtcgc ttgatgctcc aggtcctgat cgatgtcctc
     2161 gtaccggttt tcgcggtcgt cgccatcggc ttcggcctct ccaggtggat cggtatcgag
     2221 ccgcgtgctc tggcgaccct ggcctattgg gtgctcgggc ccgccttcat cttcgacgtc
     2281 ctggcgacag ccgagctcga tcccgacgtg gtcgtgaagg tggtggcaac cagcgccgca
     2341 gtcatggtcg tggccggggc cgtcgctgcc gttgtcatgc gcgtcatcgg ttcgtccttc
     2401 tcggagttgg cagcgaccgt gttgacatcg atatacggga acgccggtaa cttcggcttg
     2461 gccatcagtg c
//
LOCUS       DASUTA010000225         2108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_194545, whole genome shotgun sequence.
ACCESSION   DASUTA010000225 DASUTA010000000
VERSION     DASUTA010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194545"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VFZ80_04550"
     CDS             <1..661
                     /locus_tag="VFZ80_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006543017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX6286732.1"
                     /translation="QESIKKAMDASPGDVLLVAADRWRTAVEVLGGLRNRMGRPRGHE
                     ELAFVWIVDFPLFEEDEDGSITFSHHPFTSPVDIDQMREHPETAISKAYDAVLNGVEL
                     GSGSIRIHDPEVQRQVFEILGIDRETAQRRFGWFLEALDYGTPPHGGFAFGIDRLVMV
                     LQGESSIREVIPFPKTQTGVDPMTGAPTWVEDEQLAELGIELAPVALARREESEEEEG
                     G"
     gene            658..>2108
                     /gene="alaS"
                     /locus_tag="VFZ80_04555"
     CDS             658..>2108
                     /gene="alaS"
                     /locus_tag="VFZ80_04555"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX6286733.1"
                     /translation="MKSHEIRQAFVGFFVERDHEFRPSASLIPVDPTLLMTNAGMVPF
                     KPYFLGDEEPPWDRAVSVQKCVRTIDIDIIGTTQRHLSFFEMLGNFSFGDYFKEKAIP
                     WSYEFVTEMLGLPPERLWFTVHETDDEAHEIWVDGVGVPPDRVQRGGRDNFWQMGVPG
                     PCGPSSELFYDKGDEFGDPGGPIGGGEDRYVEIWNLVFMQNIQDEPYHVIGDLPAKNI
                     DTGMGLDRIAAVMQGVPSVFDIDTTREVLRTAERYTRLEYGASERADVSLRILADHGR
                     TVTFLIGDGVIPSNDGRGYVLRRVLRRAVRHAWQYGGEGLVIPRLSETVIDLMGDAYP
                     DLIEKRDVILDVVTREEEKFRRTLESGHQLLDTELVEGEGLSGSVAFRLHDTFGFPID
                     LTKEIAAERGVEVDLEGFDAEMEAQRSRARAAWKGGEAAGAAEVYREVLDAHGLTDFV
                     GYDFETSDGVLLSIVSDGEVIDEAEKGREVEVFL"
BASE COUNT          407 a          618 c          699 g          384 t
ORIGIN      
        1 acaggagtcg atcaagaagg ccatggacgc ctcacccggc gacgtgctgc tcgtagctgc
       61 ggatcgctgg cggacggcag tcgaggtcct cggtgggctt cgcaaccgga tggggcgtcc
      121 tcgaggtcat gaggagctcg cgttcgtctg gatcgtcgac ttcccgctgt tcgaagagga
      181 cgaggacggc tcgatcacgt tctcgcatca ccccttcacc tcaccggttg acatcgacca
      241 gatgcgagag caccccgaaa ccgccatctc caaggcatac gacgcggtcc tcaatggggt
      301 ggagctcggt tcgggctcga tcaggatcca tgatcccgag gtgcaacgac aggtcttcga
      361 gattctggga atcgaccggg agacggctca acgccgtttc ggatggtttc tcgaggccct
      421 cgactacggg acaccgccgc acggcgggtt cgctttcggg atcgaccggc tcgtgatggt
      481 gctccaggga gagtcttcga ttcgcgaggt gatccccttt cccaagaccc aaaccggggt
      541 cgatccaatg accggggcac cgacatgggt cgaggatgag cagctggcag agctcggtat
      601 cgagctcgca ccagtggctt tggccagacg agaggagagc gaggaggaag agggagggtg
      661 aagagccacg agatccgtca ggccttcgtc ggcttcttcg tcgaacgcga tcacgagttc
      721 cgaccctccg cctcgctgat cccggtggac ccgacgttgc tcatgaccaa cgccgggatg
      781 gttccgttca aaccctattt cctgggcgac gaggaacccc cctgggaccg ggcggtgtcg
      841 gtgcagaaat gcgtgcgcac gatcgacatc gacatcatcg ggacgacgca gcgtcacctc
      901 tccttcttcg agatgctggg caacttcagc tttggcgact atttcaagga gaaggccata
      961 ccttggagct acgagttcgt caccgagatg ctcggtctcc cgccagagcg tctttggttc
     1021 acagtccacg agaccgacga cgaggcacac gagatatggg tcgacggtgt cggcgtgcct
     1081 cctgatcggg ttcaacgcgg cggtcgggac aacttctggc agatgggcgt tcccggccca
     1141 tgcgggccga gctccgagct cttctacgac aaaggtgatg aattcggtga tcccggtggt
     1201 cccatcggcg ggggcgaaga ccgctacgtc gagatctgga acctggtctt catgcagaac
     1261 atccaggacg agccatatca cgtgatcggg gaccttcctg ccaagaacat cgacaccggg
     1321 atggggctcg atcgcatcgc cgcagtgatg cagggcgtgc cctcggtgtt cgacatagac
     1381 accacccgtg aggtgttgcg caccgccgag cgttacaccc gcctcgagta cggggcgtcc
     1441 gagcgcgccg acgtgtcgct tcggatcctc gccgaccacg gtcggacggt cacgttcctc
     1501 atcggggacg gggtgatccc gtccaacgac ggtaggggat acgtactgcg acgcgtcctc
     1561 cgccgcgcgg tgcgccatgc ctggcagtat ggaggagaag gtctggtcat tccgaggctg
     1621 tcggagaccg tcatcgacct catgggagac gcatatccgg atctgatcga gaagcgcgac
     1681 gtgatcctcg atgtcgtcac ccgggaagag gagaagttcc gacggacgct cgagtcgggg
     1741 catcaactgc tcgacaccga gctcgtcgag ggtgaagggc tgagtggatc ggtcgccttc
     1801 agactccacg acactttcgg atttcccatc gatctgacca aggagatcgc agcagagcgg
     1861 ggtgttgagg tggatctgga aggattcgat gccgaaatgg aggcacaacg cagccgggct
     1921 cgagcggcgt ggaaaggtgg cgaggccgcc ggcgcggccg aggtctacag ggaagtgctc
     1981 gatgcccacg ggctcaccga cttcgtcggg tacgacttcg agacttcaga cggggtgctg
     2041 ctctcgatcg tgtcggacgg cgaggtcatc gacgaagcag agaagggccg agaagtggag
     2101 gtcttcct
//
LOCUS       DASUTA010000226         2574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_194700, whole genome shotgun sequence.
ACCESSION   DASUTA010000226 DASUTA010000000
VERSION     DASUTA010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194700"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1117
                     /locus_tag="VFZ80_04560"
     CDS             56..1117
                     /locus_tag="VFZ80_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX6286734.1"
                     /translation="MSDDLFSKLFELFNQPGPVNWKLAAEIAKHLAGERQPVEPWAAE
                     EFRELARLAEYRLHDVAPFPVRPATDVLPLDPRTWAEKNLESYGSLVEPFAGSMGGEA
                     APMMAQLAPAMIGLQAGTLVGSLSAWAVAGFDAGLPPDQIGPLTLIVPNVEALVVGGV
                     DEREVRLWVVANEVAFRSVCDVAWIHDHLAQLVSDYAGAVSIDPTKLSGLMIGGTDPA
                     AIERAIEEAGGIDGLIGGEEAEAPRAELEAFLGVVTGCARLLARRAVVDMAPNFDAIS
                     RTRDEERREVTAQPAIGVGPLPPEATHLGDEFNQEVERRYGDDELQVLWADPARMPTA
                     AELRDPTAWAARVLLDGWG"
     gene            1188..1649
                     /locus_tag="VFZ80_04565"
     CDS             1188..1649
                     /locus_tag="VFZ80_04565"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX6286735.1"
                     /translation="MTLDVGTNAPDFTLLNQNREPVSLEDLKGSRSVIVFIPFAFTRT
                     CEGELCQIRDEYNLFEDAGARVVVITCNALHANRVWAEQQGFEFDILSDWWPHGEVSR
                     KYETFNETFGYAERTTYFLDGNGMITEVSRSDQLGEPRDFETYKDALTGST"
     gene            complement(1707..>2574)
                     /locus_tag="VFZ80_04570"
     CDS             complement(1707..>2574)
                     /locus_tag="VFZ80_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286736.1"
                     /translation="LDELLTRGNEATYSAEQTISCSTPDGVRDAVVRITQKDGELKVS
                     SSVTDEVEVSAGGGTWSVRSGGGLVAEAAVDAGDKEKTEPLYTVEEEEAVDYLGRAAM
                     SYLLIRDGDPRGHLTFDNETGALVEAITFTLDGEIYCERRFISIDSEVESLSAQEAPA
                     AAESAGTPPVVVESSQLPEAVAGFELLDQYEDEDGLRFSYYTDGFFSFAVFETETAVE
                     LPEAVSVELGSGEYVRAFTAGQVTYVWETVAGGMALVGDLPPDLHEAVLAEMPHPQDP
                     GFFRRWWRNLFG"
BASE COUNT          473 a          846 c          811 g          444 t
ORIGIN      
        1 tgctccggga agacagcgag ctcatcctcg aagtcactct ggggaggctg acgtcatgag
       61 cgacgacctc ttctccaagc tcttcgagct gttcaaccag ccgggaccgg tcaactggaa
      121 gctggcagcg gagatcgcca agcatctggc gggtgagcgc cagccggtag agccatgggc
      181 ggccgaggag ttcagggagc tggcccggct cgccgagtac cggctgcacg atgtcgcacc
      241 gtttccggtc aggccggcga ccgatgtgct ccctctcgac ccgcgcacct gggccgagaa
      301 gaacctggag agctacgggt ccctggtcga gccattcgcc gggtccatgg gaggcgaggc
      361 ggctccgatg atggctcaac tcgccccggc gatgatcggt ctgcaagcgg gaacactcgt
      421 ggggagcctt tctgcgtggg cggtggccgg tttcgacgcc gggctgcctc ccgaccagat
      481 cggaccactg acgttgatcg tccccaatgt cgaagcgctc gtggtcggcg gagtcgacga
      541 acgagaagtc cggctgtggg tcgtcgccaa cgaagtggca ttccgctcgg tctgtgatgt
      601 cgcctggatc cacgaccacc tggcacagct cgtctccgac tatgccggcg cggtctccat
      661 cgaccccacc aaactgagcg gcctgatgat cggcggtacg gacccggccg cgatcgaaag
      721 ggcgatcgag gaagccgggg ggatcgacgg cctcatcggc ggtgaggagg ccgaggctcc
      781 ccgagccgag ctcgaagcgt tcctgggtgt ggtcaccggt tgtgcccgtc tgctggcgcg
      841 ccgcgcagtc gtcgacatgg cgcccaactt cgacgcgatc tccagaactc gtgacgagga
      901 gcgacgtgaa gtgacggcgc agcccgctat cggcgtcggc cccctcccac ccgaggcgac
      961 acatcttggc gacgagttca accaggaggt ggagcgccgc tatggcgacg acgaactcca
     1021 ggtcctctgg gcggaccccg cgcgcatgcc caccgccgcc gagctccgcg accccaccgc
     1081 ctgggccgca cgcgtgttgt tggacggttg gggctgataa ctgcgaaccc ggggttcctg
     1141 gggccgtata tggctcgaat ccgctcatct tcgggataat gaccacgatg acactcgacg
     1201 tcggcaccaa tgcaccggac ttcacgctgc tcaaccagaa tcgcgagccg gtctccctcg
     1261 aagacttgaa ggggtcgagg tcggtgatcg tcttcatccc gtttgcgttc accaggacct
     1321 gcgagggcga gctctgccag attcgcgacg agtacaactt gttcgaggat gccggggcca
     1381 gggtggtggt gatcacctgt aacgcgctcc atgccaaccg ggtgtgggcc gaacagcaag
     1441 gtttcgagtt cgacatcctt tctgactggt ggccgcacgg agaggtgtca cggaaatacg
     1501 agaccttcaa cgagaccttc ggatacgcag agcgcaccac ctacttcctg gacggcaacg
     1561 ggatgatcac ggaggtgtcg cgatcggacc aactcgggga gccaagggac ttcgagacct
     1621 acaaggatgc cctcaccggc tcaacctgag cggatttggg ccacatatgg ccccaggaac
     1681 cccgggttcg cagatagaga ggacgttcag ccgaagaggt tgcgccacca ccggcgaaag
     1741 aagccggggt cctggggatg gggcatctcg gcgaggaccg cctcgtggag gtcgggaggg
     1801 agatcgccta ccagggccat gccaccggcg accgtctccc acacgtaggt cacctggccg
     1861 gccgtgaagg ccctgacata ctcaccgctt cccaactcga ccgatacggc ttccggcaac
     1921 tccactgcgg tctccgtctc gaatacggcg aacgagaaga agccatctgt gtagtagctg
     1981 aagcgaaggc catcctcgtc ctcgtactga tccagcaact cgaagccggc aaccgcctcg
     2041 ggcaactgcg atgactcgac tacgaccgga ggtgtcccag ccgactcggc ggcagccgga
     2101 gcttcttgtg ccgacaagct ctcgacctcg ctgtcgatgg agatgaacct ccgctcgcag
     2161 tagatctcac cgtcgagcgt gaacgtgatc gcctcgacga gcgcccccgt ctcgttgtcg
     2221 aacgtcaggt gcccccgcgg atctccgtcc ctgatgagga gataggacat cgcagcacga
     2281 cccagatagt cgaccgcctc ttcctcctcc acggtgtaga gcggctcggt cttctccttg
     2341 tccccggcgt ccaccgccgc ttcggcgacg aggcccccgc cgctgcgcac cgaccatgtc
     2401 cctccacctg cggacacttc gacctcgtcg gtcacactcg aggaaacctt caattccccg
     2461 tctttctgag tgattcgaac cactgcgtca cgaacaccat cgggtgtcga gcaactgatg
     2521 gtctgctccg ccgagtaggt ggcctcgttg ccccgcgtca gcaactcatc gagc
//
LOCUS       DASUTA010000227         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_971387, whole genome shotgun sequence.
ACCESSION   DASUTA010000227 DASUTA010000000
VERSION     DASUTA010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971387"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VFZ80_04575"
     CDS             <1..996
                     /locus_tag="VFZ80_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6286737.1"
                     /translation="GTFSSLLTGPTSAIAEQNGMVYPVPSGGAQRIWERGFENMFYFQ
                     AQPAEGVGSAPVNALLHYRDQGLISEEDFPETAAVVYADDPFAAAGRNGLAGGTVNDP
                     ATGEVLADLAPGFLADAGIEIVFDEQWPVGFTDWTTFASSIASSGAESVWAATASVDE
                     AISLVQALQQVGHQPSFVWMSQGAQSEFRDELGGAEDGITMHTVWHPTAEFVGTLGGE
                     DYSNQDFIADFTEAFGNDPSEDEAIPFAVCQGMEQAMIGAGSTDNAAMAQWLRDRTAD
                     DPVRTIMGDFHWDERGLPIGRDYLLNQWQDGTLEFIYPVGEGLDTVDMIYPKSEW"
     gene            1081..1800
                     /locus_tag="VFZ80_04580"
     CDS             1081..1800
                     /locus_tag="VFZ80_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008308439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286738.1"
                     /translation="MREISKAYGGIQAVKSCSFSVEEKSITGLIGPNGAGKTTAFDVI
                     TGIITPDEGKVLFAGTEVTDWMPHRITRRGVNRTFQITRTLGDLTVLENMVVCSRTSG
                     WRSLLRSRVLQREEDRAMDLLRFVGIDHLAFENAESLSYGQKKLLEFASVLMSEPEMI
                     MLDEPAGGVNPALLERIMDHIRVLNEQGMTFLIVEHNMDVVMTLCDTIVVMAHGEILT
                     TGTPDAIREDDTVLDAYLGRA"
     gene            1801..>2163
                     /locus_tag="VFZ80_04585"
     CDS             1801..>2163
                     /locus_tag="VFZ80_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6286739.1"
                     /translation="MALLEVDDVVAGYGAGPDILTGVSLEIEEGKSYCIVGPNGAGKS
                     TLLRTIAGFLKPRNGEIRLDGAKMNGLRPDQMLAAGVCFVPQDASLFPTMTVKENLVM
                     GGYLIEDRSLVHERLETVY"
BASE COUNT          420 a          643 c          685 g          415 t
ORIGIN      
        1 ggcactttct ccagtctgct cacgggtcct acatctgcta tcgccgagca aaacggcatg
       61 gtctacccgg ttccgtccgg tggcgctcaa cggatatggg agcgtgggtt cgagaacatg
      121 ttctacttcc aggcccagcc cgccgaaggt gtcggatccg cgccggtcaa cgctctcctt
      181 cactaccggg atcagggtct gatctctgag gaggacttcc cggagacggc ggccgtcgtc
      241 tatgccgacg acccatttgc ggcagcaggt cgcaatggcc tggcgggtgg cacggtgaac
      301 gatccagcca caggcgaagt tctcgccgat ctggcgcccg ggttcctcgc cgatgcaggc
      361 atcgagatag tcttcgacga gcaatggccc gttggcttca ccgactggac caccttcgcc
      421 agctcgattg cgagcagcgg tgcggagtcg gtttgggccg ccaccgcatc agtggatgag
      481 gcgatctcgt tggtccaggc acttcagcag gttggtcatc aaccctcctt cgtgtggatg
      541 tcccagggag cgcagtccga gttccgcgat gagcttggtg gcgcagagga cggaatcacc
      601 atgcacaccg tgtggcatcc gaccgccgaa ttcgtcggga cgctcggtgg agaggactac
      661 agcaaccagg acttcatcgc cgatttcacc gaggcattcg gcaacgaccc gagtgaagac
      721 gaggcgatcc cgtttgcggt ttgccagggc atggagcagg caatgatcgg ggcgggcagc
      781 actgacaacg cggccatggc ccagtggctg cgtgacagga cggccgacga tccggtgcgc
      841 accatcatgg gtgatttcca ctgggacgag cgcggcctcc ccatcgggcg cgactatctc
      901 ctaaaccagt ggcaggacgg aacgctggag ttcatctacc cggtcggcga gggtctggac
      961 accgttgaca tgatctatcc caagtccgag tggtaacgaa tcgaaccaga tgagggggtc
     1021 ggcgtgcgtc ggccccctcg atcaaagtgt taggagaagc acgtagctga tttgttggaa
     1081 gtgcgggaga tcagcaaggc ctatggtggt attcaggcgg tcaagtcctg ctccttctcg
     1141 gtcgaagaga agtcgataac cggtctaatc ggccccaatg gggccggtaa gactacggcg
     1201 ttcgacgtga tcactgggat catcacgccc gatgagggaa aggtgctgtt tgccggcacc
     1261 gaggtgaccg actggatgcc gcaccgcata accaggcggg gggtgaaccg cacctttcag
     1321 atcaccagga ctctcggcga cttgaccgtt ctcgagaaca tggtggtctg ctcgcggacg
     1381 agcgggtggc gcagtcttct ccggtcccgc gttctccagc gtgaagaaga ccgcgccatg
     1441 gacctccttc gtttcgtggg gatcgatcac cttgcattcg agaatgcaga gtccctgtcc
     1501 tacggccaga agaagctgct cgaattcgct tcggtcctca tgtctgagcc cgagatgatc
     1561 atgctcgatg agccggccgg aggggtgaat cccgctctcc tggagcggat catggaccac
     1621 attcgtgtcc tcaacgagca gggcatgacc tttctgatcg tcgagcacaa catggacgtg
     1681 gtgatgacgc tgtgcgatac gatcgtcgtc atggcccacg gcgagatctt gaccacgggc
     1741 acccccgatg cgatacgaga ggacgacaca gtcctcgacg cctacttggg gagggcatag
     1801 ttggctcttc tggaagtcga cgacgtcgtc gccgggtacg gggcggggcc ggacatcctg
     1861 acgggcgtga gcctcgagat agaggaaggc aagtcgtact gcatcgtcgg ccccaacgga
     1921 gcaggcaagt ccacactgct tcgcaccatc gccggatttc tcaaaccccg gaacggtgag
     1981 atccgcctcg atggggccaa gatgaacggg cttcgtcccg accagatgct cgccgccggt
     2041 gtctgcttcg tccctcagga cgccagcctc ttccccacca tgaccgtcaa agagaacctg
     2101 gtcatgggtg gctatctcat cgaagaccgc tctctggtcc acgagcggct ggagacggtc
     2161 tac
//
LOCUS       DASUTA010000228         3872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_65024, whole genome shotgun sequence.
ACCESSION   DASUTA010000228 DASUTA010000000
VERSION     DASUTA010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65024"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..471
                     /gene="rplM"
                     /locus_tag="VFZ80_04590"
     CDS             19..471
                     /gene="rplM"
                     /locus_tag="VFZ80_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017598565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX6286740.1"
                     /translation="MNKTFSPKPADIERSWHLVDATDLPLGRLASEVAQVLRGKHKPT
                     YAPHVDVGDFVVIVNADKVAVTSGKSQAKIYYRHSGYPGGIKAETFESLLERRPEAVI
                     ERAVRGMLPKNKLGRQMARKLKVYAGPDHPHAAQGPKPLELNIRKVEV"
     gene            471..863
                     /gene="rpsI"
                     /locus_tag="VFZ80_04595"
     CDS             471..863
                     /gene="rpsI"
                     /locus_tag="VFZ80_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX6286741.1"
                     /translation="MSKPLIQTTGRRKESVARVRFREGSGQVTLNGKPLETYFPTMAT
                     RMRVMEPLSLTQTQGLYDIEASLDGGGTTGQADALRLGISRGLAELDPELRATLKKAG
                     MLTRDSRVVESKKYGLRKARRAPQYTKR"
     gene            876..2225
                     /gene="glmM"
                     /locus_tag="VFZ80_04600"
     CDS             876..2225
                     /gene="glmM"
                     /locus_tag="VFZ80_04600"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009336396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX6286742.1"
                     /translation="MDLRRLLDDGKPIFGTDGVRGVAGSELTAELAMDIGRAAGSYLR
                     GGPVLVGRDTRRSGSMLSSAFQSGLHSVGIDTVDVGVLPSGGISYLTASSAATMGAVV
                     SASHNPAEDNGIKLLAARGTKLPDEVERELEGRMRSSGRQVAVGSSIGFRRENPEGYE
                     QYLAHLASASRYSLNGIGIVLDCANGAAYQAAPDLFERLNADVRVFHAEPNGLNINDG
                     CGATAPEFIARHGGGRIGLAFDGDADRLIAVDENGVIANGDVIMAIIARHLKEQGRLT
                     KNLVVATVMSNLGFRKSLADAEIEMVETPVGDRYVMEALTEHEALLGGEQSGHIIFTD
                     LASTGDGLLTAVQLLNVVAGTGKELRELRSEAIVEYPQVLVNVRVDRAVDIASIEDLW
                     NEVAVVEEELGDNGRVLVRTSGTEPLVRIMVEAPSESMASGYADRLAAVTEAATKKE"
     gene            2486..>3872
                     /gene="glmS"
                     /locus_tag="VFZ80_04605"
     CDS             2486..>3872
                     /gene="glmS"
                     /locus_tag="VFZ80_04605"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX6286743.1"
                     /translation="MCGIVGYVGPRPVVDVLVPGLAHLEYRGYDSAGIALNLGDSLDM
                     IKTAGRIADLEELVAAEGEIEAISGIGHTRWATHGAPNTTNAHPHTDQSGDVMVVHNG
                     IVENWVELKKKLIEEGHEFRSDTDTEVVAHMIGELSELPLVEAVRQVMLEAEGALALA
                     VMRKSEPELIVGARRGSPLVVGRGDGENFLASDIPAFLEHTREMVIIDDDRVVEVRPT
                     SVRIIDLDGNEIEPEHRKIEWDFDAAEKGGYATFMLKEIHEQPHAIADTLAGRVSDNG
                     RVVLNELEMDARLLRAIDKVFVVACGTSYHAAMMAKYAIERWTRLPVEIDIASEFRYR
                     DPVLDARSLVIGISQSGETIDTLAAVRYAKAQGANLIGVSNVIDSALARESDAVLYTR
                     AGLEIGVAATKTFTTQLVAMQLLGLYLAQVRGTLDPGKIELLVRDLQKLPEQVTSVLQ
                     TSDAIEELSRKF"
BASE COUNT          748 a         1203 c         1245 g          676 t
ORIGIN      
        1 ggactccaaa tcatgaccat gaacaagact ttttcgccca agcccgctga catcgagcgc
       61 tcctggcatc tcgtcgatgc cacggatctg ccgctcggtc ggctggcttc cgaggtcgcc
      121 caggtgttgc ggggcaagca caagccgacc tacgcccctc atgtcgatgt tggagatttc
      181 gtcgtcatcg tcaacgcgga caaggtcgcc gtgacctcgg gcaagagtca ggccaagatc
      241 tactaccgcc actccggata ccctggtggg atcaaggcgg agaccttcga gtctcttctg
      301 gagaggcggc ccgaggctgt catcgagcgg gctgtgcggg gcatgctccc caagaacaag
      361 ctcggtcggc agatggctcg caagctcaag gtctacgccg ggcccgatca tcctcatgcg
      421 gcccaaggcc caaagcccct cgaactgaac atccgaaagg ttgaagtctg atgtcgaagc
      481 cattgatcca gaccaccggc cgtcgcaagg agtcggtcgc ccgcgtcagg ttccgcgagg
      541 gctccgggca ggtcacgctc aacggaaagc cgctcgagac ctacttcccg accatggcga
      601 ctcgcatgcg ggtcatggag ccactcagcc tcacccagac tcagggtctc tacgacatcg
      661 aagcgtcgct ggacgggggc ggaaccacgg gtcaagccga tgcccttcgt ctcgggatct
      721 cccggggcct tgccgagctc gacccggagc ttcgggcgac cctgaaaaag gccggcatgc
      781 tcactcgcga ctcccgtgtg gtcgagtcga agaagtacgg tctgcgcaag gctcgccggg
      841 cacctcagta caccaagcgc taggagacgg ctcccgtgga tctccgtcgt ctcctcgacg
      901 atggcaagcc catcttcggg accgacgggg tccgtggggt ggcaggctcc gagctcaccg
      961 cagagctggc aatggacatc ggccgggccg ccggctccta cctccgtggc gggccggttc
     1021 tggtgggtcg ggacacccgc cgctccggat cgatgctctc gtccgcgttc cagtcgggct
     1081 tgcactccgt cgggatcgac accgtcgatg tcggcgtcct gcccagcgga ggcatctcct
     1141 atctcaccgc cagcagcgcg gcaaccatgg gtgcggtggt ctcggcgtca cacaaccctg
     1201 ccgaggacaa cggcataaag ctcctcgctg cccggggcac caagcttccc gacgaggtgg
     1261 agcgggagtt ggaaggtcgg atgcgttcct cggggcgcca ggtcgcggtc gggtccagca
     1321 tcggcttccg ccgggagaat cccgagggtt acgagcagta cctcgctcac ctcgcgtcgg
     1381 cctcgcgcta ctccctgaat gggataggga ttgtcctcga ctgtgccaac ggcgcggcct
     1441 accaggcggc gcccgaccta ttcgaaaggc tcaacgccga cgttcgcgtg ttccacgccg
     1501 agcccaacgg tctcaacatc aacgacggct gtggtgccac cgcccccgaa ttcatcgcca
     1561 ggcacggtgg gggtcgcatc ggattggcct tcgacggcga cgccgaccgg ctcatcgcgg
     1621 tcgacgagaa cggtgtgatc gccaacggcg acgtcatcat ggccatcatt gcccgtcatc
     1681 tgaaagagca aggccgattg acgaagaatc tcgtcgtggc caccgtcatg tcgaatctcg
     1741 ggtttcgcaa gtctctcgcc gatgccgaga tcgagatggt ggagactccg gtcggggacc
     1801 ggtatgtcat ggaggcgctg acggagcacg aggcgttgct cggtggcgaa cagtccggtc
     1861 acatcatctt caccgatctg gcatcgaccg gggacgggtt gctcaccgcc gtgcagctgc
     1921 tcaacgtcgt tgccgggacc ggcaaggagc tgcgggagtt gcggtccgag gcaatagtcg
     1981 aatacccaca agttctggtc aacgtacgcg tcgaccgggc ggtcgacatt gcttcgatcg
     2041 aagacctttg gaacgaagtg gcggtcgtcg aagaagaatt gggagacaac ggtagagtcc
     2101 tggtccgtac gtcgggaacc gagcccctgg tcaggatcat ggtcgaggcc ccttccgaat
     2161 ctatggcaag cggttacgct gatcgtctcg cggccgtcac cgaggcggcg acgaagaagg
     2221 aatgagcatc ggatagatcg tttgcgacag acaacacata gcgcctggcc tcgccaacaa
     2281 gcggcgaggt gacgaggagg aaggtcatcg aagtattcgg cggatgcctt cccggcccac
     2341 cagggtcgtc acggatcggg agcaaaaccc gggagcgatc ccggcacaga gacccgtccg
     2401 atctggtacc ggctgcgcgc ccacgcgcat aacagtgccg gtcctctgtc gcacccgtgt
     2461 tcctgctcca cggaaaggaa cgcatatgtg cggaatcgtc ggatatgtcg gcccacgccc
     2521 ggtcgtggat gtgctcgtcc cggggctggc acatctcgaa tatcgaggct atgactcggc
     2581 aggcatcgcc ctcaatctgg gggactcgct ggacatgatc aagaccgcgg ggcgcatcgc
     2641 cgacctcgag gagctggtgg ccgccgaagg agagatcgaa gccatctcgg gcatcggcca
     2701 cacccgatgg gccacccacg gtgcgcccaa caccaccaat gcccaccccc acaccgacca
     2761 gtcgggggac gtgatggtcg tgcacaacgg catcgtggag aactgggtcg aattgaagaa
     2821 gaagctgatc gaagagggcc acgagttcag gagcgacacc gacaccgagg tggttgccca
     2881 catgatcggt gagctctccg aacttccgct cgtcgaagct gtccggcagg tgatgctcga
     2941 ggccgagggt gctctggcat tggctgtgat gaggaagagc gagccagagc tcatcgttgg
     3001 tgcccgacgc gggtcgccac tcgtcgttgg tcgaggggac ggagagaact tcctcgcctc
     3061 cgacatcccg gccttcctcg agcacacccg tgagatggtg atcatcgatg acgaccgcgt
     3121 cgtggaggtg cgtcccacgt cggtgcgcat catcgatctc gatggcaacg agatcgagcc
     3181 cgagcaccga aagatcgaat gggacttcga cgccgccgag aagggtggct acgccacctt
     3241 catgctcaaa gagatccatg agcagcccca cgccatcgcc gacacgttgg ctggccgggt
     3301 gtccgacaac gggcgggtgg tcctgaacga gctggagatg gacgcccgat tgctgcgggc
     3361 gatcgacaag gtgttcgtgg ttgcctgtgg cacctcgtat cacgcagcga tgatggccaa
     3421 gtacgccatc gagcgttgga cccgactgcc tgttgagatc gacatcgctt cggaattccg
     3481 atacagggat ccagtcctcg atgcgaggtc gttggtgata ggcatcagcc aatcagggga
     3541 gaccatcgac actctggctg cggttcgtta cgccaaggcg cagggggcca acttgatcgg
     3601 ggtcagcaac gtgatcgact ccgcgttagc acgcgaatcc gatgccgtcc tctacacgag
     3661 ggccgggctc gagatcggtg tggcggctac gaagacgttc accacacagc tggttgcgat
     3721 gcagctgctc ggtctctatc tcgcacaggt gagggggacc ctcgatccgg gcaagatcga
     3781 acttctggta cgtgatctcc agaagctgcc cgagcaggtg acgtcggtgt tgcagacctc
     3841 cgatgcgatc gaggagcttt cacggaagtt cg
//
LOCUS       DASUTA010000229         2727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_325253, whole genome shotgun sequence.
ACCESSION   DASUTA010000229 DASUTA010000000
VERSION     DASUTA010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325253"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFZ80_04610"
     CDS             <1..358
                     /locus_tag="VFZ80_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX6286744.1"
                     /translation="PQVVHGDLHEWNVHMASSRLYAFDFEDVMLALPAQDVSICLYSS
                     RNSDIREDIRAAFRKGFESVAPWPIVDDRQLDGFHAARQIMLMNYAGRTLRMGEAADY
                     IDHVMPWLERYVKRYD"
     gene            360..998
                     /locus_tag="VFZ80_04615"
     CDS             360..998
                     /locus_tag="VFZ80_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX6286745.1"
                     /translation="MRYTDRRQAGRLLGGEIAALSPSDPVVYGLPRGGVPVAFEVADA
                     LDCPLDVLVVRKLGVPSQPELAMGALGEGGVVVSNDEIIALARIDRETFEAVVEVETR
                     ALETRVAQYRSEAPPIDPSGHTAIVVDDGLATGSTARAAIEVLRHQGAAAVWLAVPVA
                     PRGDLGGVEDTADRLVILSRPMGFGAVGVWYRDFTQTDDEEVRDLLAESRLA"
     gene            1022..1429
                     /locus_tag="VFZ80_04620"
     CDS             1022..1429
                     /locus_tag="VFZ80_04620"
                     /inference="COORDINATES: protein motif:HMM:NF023010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 family protein"
                     /protein_id="HEX6286746.1"
                     /translation="MPSLEKFCTVEASLDMRIIGQAPAGMRIDFPFEGTATSDHWAGE
                     RPVSGMDYVTVRADGNMVLDVHGTIGEKRETVAYTATGISIANEDKSADPKELIVFQT
                     GNEDLAWLNDEVGVAFGHGAGGRITLEIYLIKP"
     gene            complement(1518..2294)
                     /locus_tag="VFZ80_04625"
     CDS             complement(1518..2294)
                     /locus_tag="VFZ80_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6286747.1"
                     /translation="MTTRYELRDEVAIVTLARPERFNAIDAALSSALVADLSRAEGEG
                     RAVVLTGEGKAFCSGADLSGFVDEYDDGGPDLAKHLDEEFHPMVQAIADCRLPLVAAV
                     NGVAAGAGLGLALGCDIRVMAESAFLTSAFTAIGLAPDSGTTWLLPHHVGTSVALEMA
                     LTNRRMQADEALQRGLCVEVVPDEEVVDKAVGYAGRLAAMATDALITTRRLIRGSSTL
                     SFSDGLEAEKLEQGRLGRTPEHREGVMAFLEKREPDFRKS"
     gene            2420..2713
                     /locus_tag="VFZ80_04630"
     CDS             2420..2713
                     /locus_tag="VFZ80_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013301426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX6286748.1"
                     /translation="MNKSEMADKLATKADISKAQAAEIIDHIFSTKDGEGIIAVELDA
                     GRKVNITGFGSFSLKRRSERQGRNPATGQTITIPAKNYAHFSASKGLKDRVAE"
BASE COUNT          522 a          864 c          888 g          453 t
ORIGIN      
        1 gcctcaggtg gtccacggcg atctccacga atggaacgtc cacatggcga gcagcaggct
       61 gtacgccttc gacttcgaag acgtcatgct ggctctgccc gcccaggatg tctcgatatg
      121 tctctactcc tcacgaaaca gcgacatccg cgaggacatc cgggccgcgt tccgcaaggg
      181 cttcgagtcg gtggccccgt ggccgatcgt ggacgatcgg caactggacg gttttcatgc
      241 cgcccgccag atcatgctga tgaactatgc cgggcggacc ctgcgcatgg gtgaggcggc
      301 ggactacatc gaccacgtga tgccgtggct cgagaggtac gtcaagcgct acgactgaga
      361 tgaggtacac cgatcgccgc caggccggga gactgctcgg cggggagatc gctgcgctct
      421 caccatcaga ccccgtcgtc tacggcttgc cccgtggtgg cgtgcccgtg gcgttcgagg
      481 tggccgatgc cctcgattgc cccctcgacg tcctggtggt gcgcaagctt ggagtcccct
      541 cccaacccga attggccatg ggagccctcg gagagggggg cgtggtggtc agcaacgacg
      601 agatcatcgc tctcgcccgg atcgatcgag agaccttcga agcggtcgtg gaggtggaga
      661 cccgcgcgct ggagacacgg gtggcccagt accggtccga ggcgccgccc atcgacccgt
      721 ccggccacac cgccatagtc gtcgacgatg ggctggcgac cggctccacc gcgcgggccg
      781 cgatcgaggt gcttcgccat cagggcgccg ccgcggtctg gttggccgtg ccggtggcac
      841 cacgaggtga cctcggaggg gtagaggaca cggccgatcg tctcgtgatt ctctcccggc
      901 cgatgggctt cggggcggtt ggggtttggt atcgcgactt cacccaaacc gacgacgaag
      961 aggtcaggga cctcctcgcc gagtcccggc tagcgtgacg aaatctgctg agaggagacc
     1021 catgccgtca ctggagaagt tctgcacggt cgaggccagc ctcgacatga ggatcatcgg
     1081 tcaggcgccc gcaggcatgc gcatcgactt cccgttcgag ggaaccgcca cgagtgacca
     1141 ctgggcaggg gagcgaccgg tgagcgggat ggattacgtg accgtccgcg ccgacgggaa
     1201 catggtgctc gacgtccatg gcacgatcgg agagaagcgg gagacggtcg cctacacggc
     1261 aacggggatc tccatcgcca acgaggacaa gtcggcggat ccgaaggagt tgatcgtctt
     1321 ccagaccggc aatgaggatc tcgcctggct gaacgatgag gtcggggtgg ccttcggcca
     1381 tggcgccggc ggaaggatca ccctggagat ctacctcatc aaaccctgac cgggagccga
     1441 agccctttct gtgagtagaa cggtccagaa tctggaccgt tctactcaca gaaggtcgat
     1501 acgaggtcgg ctggccgtca cgacttcctg aagtccggct ctcgcttctc caggaaggcc
     1561 atgacaccct cgcgatgctc gggtgtccgg ccgagacgac cctgctcgag cttctccgct
     1621 tccaagccgt cggagaagct caatgtcgat gacccacgga tgaggcgacg ggtggtgatc
     1681 agggcgtcgg tggccatggc cgccagcctc ccggcataac cgactgcctt gtccaccacc
     1741 tcttcgtcgg gcactacctc cacacacagc cctcgctgca atgcctcgtc ggcctgcatc
     1801 cgtctgttgg tcaaggccat ctcgagtgcg accgaagtcc cgacatggtg gggaagaagc
     1861 caggtggttc ccgagtcggg agccaggccg attgctgtga atgccgaggt cagaaaggcg
     1921 gattcggcca tcaccctgat gtcacagccc agggcgagac cgagccccgc cccggcggcg
     1981 acgccgttga cggccgctac gagcggcagc cggcagtcgg cgatggcctg gaccatgggg
     2041 tggaactcct cgtcgagatg cttggcgagg tcgggcccgc cgtcgtcgta ctcgtcgacg
     2101 aatccggata ggtcggcccc ggagcagaag gcttttccct ccccggtgag cacgacggca
     2161 cgcccttcgc cctccgcccg gctcagatcg gcgaccagcg ccgaggagag tgcggcgtcg
     2221 atcgcgttga atcgctccgg ccgggcgagg gtcacgatcg cgacttcgtc ccgcaactca
     2281 tatctggtgg tcatttgccc tcctcggtgt cgcgaatcgt aaacgaggga aaacataggt
     2341 aaccattggg ctatccccta tcgttgggaa catcgtcgca gcagaacagc cgcgaaacat
     2401 cgacccacaa ggaggcgtcg tgaacaaatc cgagatggcg gacaagctcg ccaccaaggc
     2461 ggacatctcg aaggcacagg ccgcggagat cattgaccac atattctcga cgaaggatgg
     2521 cgaaggaatc atcgccgtcg agttggacgc gggccgcaaa gtcaatatca ccggattcgg
     2581 cagcttcagc ctcaagcgcc ggtcggagcg tcaggggcgc aacccggcaa cagggcagac
     2641 catcaccatt cccgccaaga actacgcaca tttcagtgcc tccaaaggcc tgaaggatcg
     2701 agtggccgag tagcaaccga cagtcag
//
LOCUS       DASUTA010000230         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_325345, whole genome shotgun sequence.
ACCESSION   DASUTA010000230 DASUTA010000000
VERSION     DASUTA010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325345"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2358
                     /locus_tag="VFZ80_04635"
     CDS             <1..>2358
                     /locus_tag="VFZ80_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6286749.1"
                     /translation="VSTVILVAVLALLPVALALGGFLLTRSILTRRSLTRQLEISDAL
                     AAERQRRVEVLSDYELITENSADLIARQSPDLTYLWVSPSIRNVLGYAPAEAIGTDPW
                     SVIHADDAEKVETARRELADGATTSSVEHRARHANGKWVWLHTVSRAVRDEDGRLVEI
                     QSASRDVSEQVRQRSELIESKNAVEMAAAEKTRFLSTVSHEIRTPLTAVRGLTDLLLE
                     SELDQDQREQVETIRLASTDVMTLVNDLLDLAKAEAGHLRLDAAPFDLMVTIDNVIRV
                     FAAQAEAKQLLLRSDVRNAPVGLIGDSHRLHQILVNLIGNALKFTSEGSITLRAEPLG
                     HDGESQVTIHFEVADTGLGIPGQRLDAIFEEFEQAESSTARNFGGSGLGLGISKELVQ
                     LMGGVIGVESEEGAGSTFWFDIPFPLAQPTDAGWRRCRVVGRPMTRAPLAAMLRSNGW
                     LIEEMDGIETIADVSGPVVFGGTIDDLNTIQIPEKLTFVDTLPQRGNADRARHLGARG
                     YLGSPVGVDDLAYVVNGVAEGALFATRHEIEANARPLLVLAADDAPSNRLLLERTLGK
                     RGHRVVAVPGGVRALEEVLSPTPYDVVILDGQMPDLSGMEVARRIRATESGTDRHIPI
                     VALTGRVSEQEKAEVLDAGADVWVGKPFDASSLHEVIESLAMAHPLRPRPVEGAVEPS
                     VDVELFMSQSGGMPDLAAELLEVARREWDELAPSLQPQAVAQDLETAARSAHRVKGVL
                     GMLGAVGAARCAAELEKAANAEEVIEARAAAEELQAAYLDAERALL"
BASE COUNT          443 a          745 c          774 g          396 t
ORIGIN      
        1 cgtcagcacc gtcatcctgg tggcggtcct cgcactgctg ccggtggccc ttgccctggg
       61 cggcttcctg ctcaccaggt cgatcctcac ccgaagatcg ctgacccgcc agctcgagat
      121 atccgacgcc ctggccgcgg agagacagcg ccgggttgaa gtcctctccg attacgagct
      181 gatcacggag aattctgccg atctcatcgc caggcaatcc cccgatctca cctatctttg
      241 ggtctctccc tcgatcagaa acgtcctcgg gtacgccccg gccgaagcaa tagggaccga
      301 cccgtggagt gtcattcatg ccgatgacgc cgagaaggtc gagacggcgc gacgggagct
      361 ggcggatggt gccacgacct cctcggtgga gcaccgcgcc cggcatgcca atggcaagtg
      421 ggtctggcta cacaccgtgt cccgggcggt ccgcgacgag gacggtcgtc tcgtggagat
      481 ccagtccgcc tccagggatg tcagcgaaca ggtgagacag cgttccgagt tgatcgagtc
      541 caagaatgcc gtcgagatgg ccgcggccga gaaaacgagg tttctgtcca cggtcagcca
      601 cgagatcagg actcccctca ccgcggtgag agggctcacc gacctcctct tggagagcga
      661 gctcgaccaa gatcagcgag agcaggtcga gacaatccgc ctggcgagta cggacgtgat
      721 gacactcgtc aacgatctcc tcgatctcgc caaggcagaa gctgggcatc tccgcctcga
      781 tgccgcgccg ttcgacttga tggtgacgat cgacaacgtc ataagggtgt ttgcggccca
      841 ggcagaggcc aagcagctgc tactgcgctc cgatgtgagg aacgctccgg tcgggctcat
      901 cggggactca catcgcctcc atcagatact ggtcaacctc attggcaacg ccctcaagtt
      961 cacctcggag ggaagcatca ccctaagggc cgagccgctt ggccatgatg gggagagcca
     1021 ggtcactatc cacttcgagg tggccgacac cgggctcggc ataccgggtc aacgcctcga
     1081 tgccattttc gaggagttcg agcaggcgga gagctcgacc gcccgcaatt tcggaggcag
     1141 cggcctcggt ctcgggatct ccaaggagct ggtccagttg atgggcgggg ttatcggagt
     1201 cgagagcgag gagggcgcgg gctcaacgtt ctggttcgac atcccgttcc cgctggctca
     1261 accgactgac gccggctgga gacggtgcag agtcgtcggc cgaccgatga ccagggctcc
     1321 gctggcggcg atgctgaggt ctaacggatg gctgatcgag gagatggacg gaatcgagac
     1381 catcgccgac gtctccggac cggtcgtgtt cgggggcacg atcgacgacc tcaacacaat
     1441 ccagatcccc gaaaagctga ctttcgtcga caccttgccc cagcgcggca acgccgaccg
     1501 cgctcgccac ttgggcgcaa gggggtatct ggggtcgccg gtcggggttg atgatctggc
     1561 atacgtcgtc aacggtgttg ccgaaggggc tctcttcgcc acccgccacg agatcgaggc
     1621 caacgcccga ccgcttctgg ttctcgctgc agacgacgct ccctccaacc gtcttctcct
     1681 tgagcggaca ctgggcaagc gcggacacag ggtggtggca gtccctggcg gagtccgagc
     1741 cctggaagaa gtgctgtccc ctacgcctta cgacgtggtg atcctcgacg gccagatgcc
     1801 cgatctgagt ggcatggaag tggcccgccg catccgagct accgagtccg ggaccgatcg
     1861 ccacatccca atcgtggcgc tcaccgggcg agtgagtgag caggaaaagg ccgaggtgtt
     1921 ggatgctgga gcggacgtct gggtcggcaa accgttcgac gcgagctcgc tccacgaagt
     1981 gatcgaaagc ctcgcgatgg cgcacccact gaggccaagg ccggttgagg gggccgtgga
     2041 accgagcgtc gacgtcgagt tgttcatgag ccagtccggc ggaatgcccg acctcgccgc
     2101 agagctcttg gaggtcgccc gacgcgaatg ggatgagttg gcaccgtcgt tgcagccaca
     2161 agcagtggcc caagacctcg agaccgccgc tcggagcgcc caccgggtca agggtgtgct
     2221 tggcatgctc ggcgcggtcg gggccgcacg atgcgcagcc gagctcgaga aggcggcgaa
     2281 cgccgaagaa gtcatcgagg cgcgtgccgc cgcagaggag ctgcaggctg catacctcga
     2341 cgcggagcgt gcccttct
//
LOCUS       DASUTA010000231         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1102377, whole genome shotgun sequence.
ACCESSION   DASUTA010000231 DASUTA010000000
VERSION     DASUTA010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102377"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VFZ80_04640"
     CDS             complement(<1..512)
                     /locus_tag="VFZ80_04640"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEX6286750.1"
                     /translation="MNPKVETLTDGHRWLRVADPLWSDPLDPSFAQSHGGRWNPPGSF
                     PTLYLNEDLHTARAQVVALLRGSPVEPDDLDDGFDLVTATLPRAQEVADAVDDEGLES
                     LGLPETYPRYGNGRPIRHDVCQGIGLAVKSAGLRGVHARSAATDDGSGRELAWYPARE
                     SSRAKLEERRS"
     gene            complement(509..961)
                     /locus_tag="VFZ80_04645"
     CDS             complement(509..961)
                     /locus_tag="VFZ80_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286751.1"
                     /translation="MRLRENGPMDISLAPDELARELVDSADADWLRTLVDALDREVRT
                     APLSRLVSLWDLSNAGAARIFGVSRQAFSKWLAAGPPADRVDDVAAVDNITGLLDRYV
                     KRERIAAVVRRRSENLGDRSIIECLEAGEYQQTASQVRATFDLRRVQP"
     gene            1066..1491
                     /locus_tag="VFZ80_04650"
     CDS             1066..1491
                     /locus_tag="VFZ80_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX6286752.1"
                     /translation="MAELKTTRSDADVDAFLASVDNDLRRRDALAMRDLMTEVTGEKP
                     EMWGNAIVGYGPYTYRPKAGSAEHEWFKVGFSPRKQYLTLYVMDGFSEYEDLLSRLGS
                     HTTGKACLYIKDLEKVDKDILSELIARSVAHVEGLAASD"
     gene            complement(1495..>2146)
                     /locus_tag="VFZ80_04655"
     CDS             complement(1495..>2146)
                     /locus_tag="VFZ80_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286753.1"
                     /translation="PLATAKDGVPINAVLIDTGNNTKSDLTEGLMSGVNFSDGLDARL
                     TRSTNTRSVDGYQLDNNALWKYLMRSSDHGIALCDGDTIRGLSTIEEKNDAMRACLEF
                     YATTYPAPAVGPQIITDAILESPRVGVAPRLWHDNLGSGISYRPVKQFDVVYIHGLWF
                     NDDTFFPDDGNTPIALKKTEIQQVTAYLLMDSMVSANVHDQLGGFANDTWQPEIYE"
BASE COUNT          402 a          680 c          660 g          404 t
ORIGIN      
        1 gaccgtcgct cctcgagctt ggctcgcgat gactcccgag ccggatacca ggccaattct
       61 ctacctgatc cgtcgtccgt ggcggccgac ctggcgtgga ctccgcgcag ccccgcgctc
      121 ttgaccgcca gtccgatccc ctgacacacg tcatggcgga tcggacggcc attcccatac
      181 ctgggatatg tctcgggcaa tcccagactc tccagccctt cgtcgtcgac ggcatctgcc
      241 acctcttggg ctctgggaag ggtcgccgtg acgaggtcga agccatcgtc gaggtcgtct
      301 ggctccaccg gcgagccccg gagcaacgcg accacctggg ctcgggccgt gtgcagatcc
      361 tcgtttaggt agagggtcgg gaacgagccg ggcggattcc aacgcccacc atgactctgc
      421 gcgaacgacg gatcgagtgg atcggaccac aggggatcgg ccaccctgag ccacctatgc
      481 ccatcggtca gggtctcgac cttcggattc atggctgcac tctgcgcagg tcgaaggtcg
      541 ctctgacctg actggccgtc tgctgatact cccccgcttc cagacactcg atgatcgacc
      601 gatcgcccag attctctgac cgacgtctca ccaccgcggc gatcctctct cgcttgacat
      661 acctgtcgag cagcccggtg atgttgtcca cggccgccac atcgtccaca cggtcggcag
      721 gaggccccgc ggcgagccat ttcgagaaag cctgcctcga aacaccgaag atgcgggccg
      781 ccccggcgtt ggagagatcc cacaacgaga cgagccgtga cagaggggcg gttctcacct
      841 ctcgatcgag cgcgtcgacg agggtccgca gccaatcggc atcagccgag tcgaccagct
      901 ctcgggccag ctcgtcgggg gccagggaga tgtccatggg cccattctcg cgcaaccgca
      961 tgtcaaccgc aaccatatgt caacctagat ctgcgagtcg ttccacaggt gacgattgtg
     1021 cccgggccca ccgacagatt caccggcggc gcgtatcgtt gcggcatggc agaactgaag
     1081 accacccgaa gcgatgccga cgtcgacgcg tttctcgcct cggtcgacaa cgacctgcgc
     1141 agacgcgatg cacttgcgat gagagacctg atgactgagg tcaccgggga gaaacccgag
     1201 atgtggggta atgcgatcgt tggctacggc ccctacacct accgtcccaa ggccgggagt
     1261 gcggaacacg agtggttcaa ggttggcttc tccccgagga agcaatatct gacgctctac
     1321 gtcatggacg ggttctccga gtacgaggac ctgctctccc ggctcgggag tcacaccaca
     1381 ggcaaggcct gcctgtacat caaagacctc gagaaggtgg acaaggacat cctctccgag
     1441 ttgatcgccc gctcggtggc acacgtcgaa gggctggcag cgtcggactg aggcctattc
     1501 gtagatctcc ggctgccacg tgtcgttggc gaagccgccc agttggtcgt ggacattggc
     1561 ggaaaccatg ctgtccatca ggagataggc ggtcacctgc tggatctcgg tcttcttcaa
     1621 cgcgatcggg gtgttgccgt catcggggaa gaaagtgtcg tcgttgaacc agagcccgtg
     1681 gatgtaaacc acatcgaatt gcttcaccgg ccggtatgag atcccactgc ccagattgtc
     1741 gtgccagaga cggggggcaa ctcccacccg gggtgactcg agaatcgcat ctgtgatgat
     1801 ctgggggccc acggccggcg ccggataggt ggtggcgtag aactcgagac acgctcgcat
     1861 ggcgtcgttc ttctcttcga tggtggacag gccacggatg gtgtcgccgt cacacaacgc
     1921 gattccgtgg tccgacgacc tcatcaagta cttccagagg gcattgttgt ccaactggta
     1981 tccgtcgaca ctgcgggtgt tcgtcgagcg tgtgagccgg gcatccaggc cgtcggagaa
     2041 gttgaccccg ctcataagac cctcggtgag atcagacttg gtgttgttcc cggtgtcgat
     2101 gaggaccgcg ttgatcggga caccgtcctt agccgtggcc agtggt
//
LOCUS       DASUTA010000232         2286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1491123, whole genome shotgun sequence.
ACCESSION   DASUTA010000232 DASUTA010000000
VERSION     DASUTA010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491123"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1490
                     /locus_tag="VFZ80_04660"
     CDS             144..1490
                     /locus_tag="VFZ80_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6286754.1"
                     /translation="MNKPKPWRYLAIITVLALLLAACGGDGEGGDTTEAAPDTTEAPS
                     DTTGTTAPMTDTTTAGGEPEGEPIVVGGTLSLTGFLAPTAAIHHAVGEVFVDRINAQG
                     GLLGRPVEWQVLDDESVPDQAAALYERLITEDEVDLVLGPYGTGNITAAMAVAERYGY
                     MFPHHTASLTYAYTYACHFSSWSTGLHPNITNPNLVYDAIESSGNTPETIGFVINEFP
                     GTQFIAYGQPGTDEGGAVAEAANRGYDVVLEVSFPTAITDWAPIAAQVADADPDFLYV
                     GGLGVDASNLITAMNAIDYSPSGMFTQWDAPGPLLGTEGSDGVMSVTIFEQHPPFIDD
                     PEVVEVAADINQALADAGIPFTDVETQAAASWTAWEYLQQAAEGAGSLDQQAMCDYLL
                     ENGAQTLFHGDIEFYPEEQNYYVDLSKIKQIQDGGWYVVYPEEFAAPDRSVIYPLN"
     gene            1571..>2286
                     /locus_tag="VFZ80_04665"
     CDS             1571..>2286
                     /locus_tag="VFZ80_04665"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6286755.1"
                     /translation="MDLLVQGIVTGVLIGGLYALMSLGISLSWGALKIINLAHFSFIL
                     LSAYLVYQLTVTFEVDPLLMALVVSPVFFVVGVLIQMFFHWTKIDEFKSLIVTFGIFI
                     ILQSGMQTIWSADFRRIDSSLNSYAGGSITIAGIPLPFPVLLSFIAAVAIAVAMSLVL
                     GRTYFGKAVRAVAQDPEMARAYGVDTRRVAVLLSGLSGMFSALAGVFIALGQAIFPGL
                     AVSWFGIVFSVVILGGLGNTV"
BASE COUNT          416 a          713 c          692 g          465 t
ORIGIN      
        1 taacgaacac tgaaaccggt cggcatgacc gttggtcatt ggccaacacc gcgatttctc
       61 ttaagtgcca ctacacggtg tgagatcgtt gtgtagtgtc gctgaacagc gttccggcgt
      121 gagtcctgtg aggaggatta cggatgaaca aaccaaagcc atggagatat ctcgccatca
      181 tcacagtcct ggcgctcttg ttggcggcat gcggcggcga cggagagggc ggagacacta
      241 ccgaggccgc gccggacacg acggaggcgc catcggacac gacaggcacc actgcaccga
      301 tgaccgatac gaccacggcg ggcggggagc ccgagggcga gccgatcgtg gtcggcggga
      361 cactgtcgct aaccgggttc ctggccccga cggctgcgat ccatcatgcc gtcggcgagg
      421 tattcgtgga ccggatcaac gctcagggag gcctgctcgg gcgtcccgtc gaatggcagg
      481 ttctcgacga cgagtccgtt cctgaccagg cggcagccct ctacgagcgt ttgatcacag
      541 aggacgaggt cgacctggta ctcgggcctt atggcaccgg caacatcacg gcagcgatgg
      601 ccgtcgccga gcgctacggg tacatgttcc cccatcacac ggcgagcctg acctacgcgt
      661 acacctatgc ctgccacttc agctcttggt cgaccggcct gcaccccaac atcaccaacc
      721 ccaacctggt gtatgacgcg atcgagagca gcggcaacac cccggagacg atcggcttcg
      781 tcatcaatga gttcccggga acgcagttca tcgcctatgg gcagccgggc acagacgaag
      841 gcggtgcagt cgccgaggcg gcgaaccgtg gctacgacgt cgtgctcgag gtcagcttcc
      901 cgacggcgat caccgactgg gcgccgatag cagctcaggt ggccgacgcc gaccccgact
      961 tcctctacgt cggaggtctt ggggtcgacg cctccaacct gatcaccgcg atgaacgcaa
     1021 tcgactactc cccgagtggc atgttcaccc aatgggacgc accaggcccg ctgctcggga
     1081 ccgaaggctc cgacggcgtc atgtcggtga ccatcttcga acagcatccg ccgttcatag
     1141 acgatcccga agtggtcgag gtggccgccg acatcaatca ggcactggcg gacgccggga
     1201 tcccgttcac cgacgtggag acccaagcag ccgcctcgtg gaccgcatgg gagtacctac
     1261 agcaggccgc cgagggagcg ggaagcctgg accagcaggc gatgtgtgac tacctgctcg
     1321 agaacggggc ccagaccctg ttccacggtg acatcgagtt ctacccggag gagcagaact
     1381 actacgtcga cctgtccaag atcaagcaga tccaggatgg tggctggtac gtcgtgtacc
     1441 ccgaggagtt cgccgcaccg gatcgctcgg tgatctatcc cctgaactag aacctatgtc
     1501 tgggccggcc gctctcgggc ggccggcccg aagtagggtc gattacgtcc ccatcgagga
     1561 ttagacaagc gtggatctgc tcgtccaagg aatcgtcacg ggtgtgctga taggcggact
     1621 ctatgccctt atgtctctgg gcatatcgtt gtcgtggggc gccctcaaga tcatcaatct
     1681 cgctcacttc tcgttcatcc tgctcagcgc ctacctcgtc taccagttga ccgtcacctt
     1741 cgaggtcgac ccgttgctga tggctcttgt ggtctcccca gtgttcttcg tcgtcggcgt
     1801 tctcattcag atgttcttcc actggaccaa gatcgacgag ttcaaatcgc tgatcgtcac
     1861 cttcgggatc ttcatcatcc tgcaaagcgg gatgcagacg atatggagcg ccgatttccg
     1921 ccgcatcgac tccagcttga actcttacgc aggcggctcg atcaccatcg ctggtatccc
     1981 tcttcctttt cctgttctgt tgtcgttcat cgccgctgtc gcgattgctg tcgccatgtc
     2041 gctggtgctg ggtcgcacct atttcggcaa agcggtgaga gctgttgccc aggatccgga
     2101 gatggctcgt gcctatggtg tcgacacacg ccgcgtcgct gtcttgcttt ccggtctgtc
     2161 cgggatgttc tcggcgttgg ccggggtctt catcgcgctc gggcaggcca tttttccggg
     2221 tctggcggtg agctggttcg ggattgtctt ctccgttgtg atcctcggtg ggttgggcaa
     2281 caccgt
//
LOCUS       DASUTA010000233         2839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_584572, whole genome shotgun sequence.
ACCESSION   DASUTA010000233 DASUTA010000000
VERSION     DASUTA010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584572"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /gene="rplB"
                     /locus_tag="VFZ80_04670"
     CDS             complement(<1..692)
                     /gene="rplB"
                     /locus_tag="VFZ80_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002528761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX6286756.1"
                     /translation="MAVKKRKPTSAGRRFQTVSDFAEITDSKPEKSLIAKKSKTGGRN
                     TNGRVTSRHRGGGHKQRYRIIDFKRDKDGIPARVASIQYDPNRNARIALLNYADGEKR
                     YILTPVGVGIDDRLESGSGADIRPGNALPLRNVPAGTMVHAIEMRPGGGAKMARSAGS
                     AVQLMSKEGDLALLRLPSGEMRQVPLDCKATVGQVGNTEAELIKLGKAGRNRWKGVRP
                     QTRGVAMNPVDHP"
     gene            complement(694..981)
                     /gene="rplW"
                     /locus_tag="VFZ80_04675"
     CDS             complement(694..981)
                     /gene="rplW"
                     /locus_tag="VFZ80_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX6286757.1"
                     /translation="MRTAHDIILAPVVSEKSYDLIERNNTYTFVVDPRATKPQIRDAV
                     ESVFDVKVLRVNTMNRKGKVKRTGWKLGKRKDIKRAVVTLAEGDSIDLFGV"
     gene            complement(978..1667)
                     /gene="rplD"
                     /locus_tag="VFZ80_04680"
     CDS             complement(978..1667)
                     /gene="rplD"
                     /locus_tag="VFZ80_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004601445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX6286758.1"
                     /translation="MADVKAPLFKADGAANGDIKLDGDVFGIEPNLDVLHQVVTAQLA
                     AARAGTASTKSRGEVRGGGRKPWRQKGLGRARHGSIRSPIWSGGGIAHGPKPRSYAQR
                     TPKKMKRLALCSALSARASEAAIKVVDDIDWAAPKTKQAVTLLNAMEAQGKTLVVLTH
                     LDGSAGRSFRNLPDVRMVEPGHLTAYDVLWSDQVVFTSHTVGSVGRSSKYEVSEGDFV
                     KENNRDGGEEE"
     gene            complement(1660..2373)
                     /gene="rplC"
                     /locus_tag="VFZ80_04685"
     CDS             complement(1660..2373)
                     /gene="rplC"
                     /locus_tag="VFZ80_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005193581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX6286759.1"
                     /translation="MLPLRLERRVRGGSPLLGDHGNRAVSSLNGILGTKLGMTQIYDD
                     EARSIPVTVVKAGPVHVVQIKTIENDGYNAIQISYGESGSVNKPTAGHYAKAGVAPGN
                     HLLEIKVADTSEFTVGQEIKVEEMFQVGTKADVSGVSKGKGFSGVMKRHNFSGQGASH
                     GNHKKHRAPGSIGACATPSRVFKGMRMAGRMGGEKVTTLNLEVAGVDAERGLLLLNGS
                     VPGPKGSVVFVREAVKAHG"
     gene            complement(2390..2698)
                     /gene="rpsJ"
                     /locus_tag="VFZ80_04690"
     CDS             complement(2390..2698)
                     /gene="rpsJ"
                     /locus_tag="VFZ80_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX6286760.1"
                     /translation="MAEQKIRIRLKAYDHNVVDESSKKITETVLRTQAKIRGPVPLPT
                     EIHRYCVVRGPHVHKDSREHFEMRIHKRLIDILEPTPKTVDSLMRLELPAGVEVEIKL
                     "
     gene            complement(2698..>2839)
                     /locus_tag="VFZ80_04695"
     CDS             complement(2698..>2839)
                     /locus_tag="VFZ80_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX6286761.1"
                     /translation="QMVMPGDNTEMTVELIAPIAMDEGLRFAIREGGRTVGAGRVVKI
                     TK"
BASE COUNT          483 a          904 c          868 g          584 t
ORIGIN      
        1 ggatggtcga ccgggttcat tgcgacaccg cgggtctggg gacgcacacc cttccagcgg
       61 ttccgacccg ccttgcccag cttgatgagc tcggcttctg tgttgccgac ctggccgaca
      121 gtggccttgc agtcgagcgg aacctgacgc atctccccgg agggaagtcg gagcagggcg
      181 aggtcgccct ccttggacat caactggaca gccgagcccg cagagcgagc catcttcgcc
      241 cctccccctg ggcgcatctc gatggcgtga accatcgtcc cagcggggac gttgcgcaat
      301 ggcagggcgt tgcccggccg gatgtcggcg ccggagcccg actcgagacg gtcgtcgatg
      361 ccgaccccga cgggagtgag gatgtagcgc ttctccccgt cggcatagtt gagcagagcg
      421 atgcgagcgt tgcggttcgg gtcgtactga atcgaggcga cacgagcggg aatgccgtcc
      481 ttgtcgcgtt tgaaatcgat gatccgataa cgctgtttgt gaccgccgcc gcgatgtcgc
      541 gaggtaacgc ggccgttggt gttgcggcca ccggtcttgg acttcttcgc tatcagcgac
      601 ttctcgggct tcgaatccgt gatctcggcg aaatccgaga ccgtctggaa acgacgcccg
      661 gcagatgtcg gctttctctt cttgactgcc atctcagact ccgaacagat cgatcgaatc
      721 accttcggcg agggtgacca cggcgcgctt gatgtccttg cgcttcccca gcttccagcc
      781 ggtgcgcttg accttgcctt tgcggttcat cgtgttcacc ctgaggacct tcacgtcgaa
      841 gacgctctcc accgcatccc gtatctgagg cttggtggcc ctggggtcca ctacgaaggt
      901 gtatgtgttg ttgcgctcga tgaggtcata ggacttctcc gagacgaccg gagcgaggat
      961 gatgtcatgg gcagttctca ctcttcctca cctccgtcgc ggttgttctc cttgacgaaa
     1021 tcgccctctg agacctcgta cttgctggac cggcccacgg agcccaccgt gtgtgatgtg
     1081 aagaccacct gatctgacca aagaacgtcg tatgcggtca gatggccggg ctccaccatg
     1141 cgcacatcgg gcaggttcct gaaagaccgg ccggccgacc catcgaggtg ggtgagcaca
     1201 acgagggtct tgccctgggc ctccatcgcg ttcaagagcg tgacggcctg cttggtcttg
     1261 ggcgcagccc agtcgatgtc gtcgacgacc ttgatggccg cctctgaggc tcgagcggag
     1321 agggcactgc acagtgccag tcgcttcatc ttcttcggtg tccgctgggc atagctgcgg
     1381 ggcttgggcc cgtgggcgat accgccaccg gaccagatgg gagagcgaat cgagccgtgc
     1441 cgggcccgac cgaggccctt ttggcgccac ggcttgcgac caccgccgcg gacctctcct
     1501 cgagacttcg tcgaggccgt cccggcacgg gccgcggcga gctgagccgt caccacctga
     1561 tgcaggacgt cgaggttggg ctcgatcccg aagacatcgc cgtcgagctt gatgtcgcca
     1621 ttggcggcgc cgtccgcctt gaacagaggg gctttcacgt cagccatgag ccttcaccgc
     1681 ttcccgcacg aaaacgacag agcccttggg cccgggaacc gagccgttga gaagcagcag
     1741 accgcgctcg gcgtcgacac cggccacctc gagattgaga gtggtcacct tctcgccacc
     1801 catccggccg gccatgcgca tgcccttgaa cacgcgagaa ggggtggcgc aagcgccaat
     1861 ggaaccggga gcgcggtgct tcttgtggtt tccgtgcgac gcaccctgac ctgagaagtt
     1921 gtgccgcttc atgacaccgc tgaagccctt gcctttggag accccactga cgtcggcctt
     1981 ggtaccgacc tggaacatct cctcgacctt gatctcctgg cctacggtga actcagatgt
     2041 gtccgccacc ttgatctcga ggaggtggtt cccgggggcg acacctgcct tggcatagtg
     2101 gcccgcggtc ggcttgttca ccgaacctga ctcgccgtat gagatctgga tcgcgttgta
     2161 accgtcgttt tcgatggtct tgatctggac cacgtggacc ggtcccgcct tgaccacggt
     2221 gaccgggatc gaccgggcct cgtcgtcata gatctgggtc atgcccaact tggtgccgag
     2281 gatgccgttg agggagctca cagctctgtt tccatgatca ccgaggaggg gcgacccccc
     2341 tcgaaccctc cgctcgaggc ggagtggcag catcggagct gaacagtcgt cataacttga
     2401 tctccacttc cacgcctgct gggagctcga gacgcatcaa tgagtcgacc gtctttggcg
     2461 tcggctcgag gatgtcgatg agccgcttgt ggatacgcat ctcgaaatgc tccctcgagt
     2521 ccttgtgcac atgtggacca cgcaccacgc aatagcgatg gatctccgtc ggcaatggca
     2581 ccgggcccct gatcttggcc tgagtgcgca gcaccgtctc ggtgatcttc ttcgaagact
     2641 cgtcaaccac gttgtggtcg taagccttca agcggatgcg gatcttctgc tcggccatta
     2701 cttggtgatc tttacgactc gtccggcgcc gacggtgcgg ccaccttccc ggatagcgaa
     2761 ccggagacct tcgtccatag caatcggagc tatcaactcc accgtcatct cagtgttatc
     2821 accaggcatc accatctgg
//
LOCUS       DASUTA010000234         2438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_196645, whole genome shotgun sequence.
ACCESSION   DASUTA010000234 DASUTA010000000
VERSION     DASUTA010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196645"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1460)
                     /locus_tag="VFZ80_04700"
     CDS             complement(30..1460)
                     /locus_tag="VFZ80_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6286762.1"
                     /translation="MTASSPVLTFLGGAGTVTGSKTLLDTPNGRILVDCGLFQGKKHL
                     RLQNWAPFPVPPESVDAVVLTHAHVDHCGYLPRLVRLGFRGPVFCTEGTQKLAEIVLP
                     DSGHLQEEEARFANLKGYSKHDPAEPLYTRQQALAALDQFVTVGAGEPREVLDGLNAT
                     WHRAGHILGAASVEVHLTEQDIVARFSGDLGRSSHPLLLPPAPIDKADVVVIESTYGD
                     ETHLKTDPDEAIATAVMHVVASKGVLIIPAFAVDRTEVVLWHLDRLITKGLIPKVPVF
                     VDSPMACRALDLYDEAARGGAAEIRPEFRETELFADLRPTEITTVEESKALNGRGGPM
                     IVVSASGMATGGRVIHHLAQRIGNEGNAVLLVGFQAPGTRGDALANGAPRIKMFGQYF
                     PVRAPIFSVELSAHADREELMTWLSTASPPARIVFVNHGEPSASETLARAIESKLGID
                     AVVPQQGERVRLDPLPTDTRVRGFGS"
     gene            complement(1639..>2438)
                     /locus_tag="VFZ80_04705"
     CDS             complement(1639..>2438)
                     /locus_tag="VFZ80_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX6286763.1"
                     /translation="ADAEAAEAFGIAPGMLTVMIHCGSRGLGHQTCTDQLRLMGEAMQ
                     RHGIHVPDRQLACVPIDSSEGRRYLGAMAAASNFAWANRQILAHEARQGFAAALGVPP
                     EATGMSLVFDVAHNLAKMESHEVGGRATRVCVHRKGATRAFGPGHPDLPADFADYGQP
                     VLVPGSMGTASWVLRGVEGNPAFASAAHGAGRLMSRKKASRRSPGRSVQMSLQAAGIS
                     VRPRSVRLLSEEAPYAYKDVDAVADVCEAAGLASKVARLRPLGVVKG"
BASE COUNT          451 a          802 c          728 g          457 t
ORIGIN      
        1 gtttatcccg cccacaacga caatccggct catgacccga agccgcgaac tcgagtgtcg
       61 gtcgggaggg gatcgagcct cacccgctct ccctgctggg gtacgaccgc gtcgatccct
      121 agcttggact cgattgctcg agccagtgtt tcggaagcgg atggctcccc gtgattcaca
      181 aagacgatcc tcgctggcgg tgaagccgtc gagagccagg tcatcagctc ctcgcgatcg
      241 gcatgggccg aaagctccac ggagaagatg ggtgctctga ccggaaaata ctgaccgaac
      301 atcttgattc tcggtgcccc attggccaag gcgtctcccc gtgtaccagg tgcctggaat
      361 ccgacgagca acacggcatt cccctcgttg ccgatccgct gtgcgagatg gtggatgact
      421 cttcccccgg tcgccatccc cgaggcggag acgacgatca tcggcccgcc tcgaccattt
      481 agagccttcg attcctcgac ggttgtgatc tcggtcggcc ggaggtcggc aaagagctct
      541 gtctcccgga attcgggacg gatctcagcc gctcccccgc gcgccgcctc gtcgtacaga
      601 tcgagggccc gacaagccat ggggctgtcg acgaagacag gcaccttggg aatcagaccc
      661 tttgtgatca gccgatccag atgccacaga accacttcgg tcctgtcgac cgcgaaggcg
      721 ggaatgatca agactccttt gctggctaca acgtgcatga ccgcagtcgc gatcgcctca
      781 tcgggatcgg ttttcagatg agtctcgtct ccgtatgtcg actcgatcac gactacgtcg
      841 gccttgtcga tgggtgccgg tggcaggagc agtgggtgac tgcttctacc caggtcgcca
      901 ctgaatctgg caacgatgtc ttgttccgtg agatgaacct ccacagaagc tgctcccagg
      961 atgtgaccgg ctcgatgcca ggtggcgttc aatccatcca ggacttctct tggctcaccg
     1021 gcgcccactg tgacgaattg gtccagggcg gccaaagcct gctgcctggt gtagagcggt
     1081 tcggccggat cgtgcttgga gtatcccttc aggttggcga atcgcgcttc ttcctcctga
     1141 agatgaccgc tgtcgggaag cacgatctcg gccagtttct gagtgccctc agtgcagaaa
     1201 accggacccc ggaagccgag ccggaccagt ctcggcaagt agccacagtg gtcgacgtgg
     1261 gcgtgagtca gtacgacggc atcgaccgac tcagggggca ccggaaaggg cgcccagttc
     1321 tgcagacgca ggtgcttctt gccctggaag agaccgcagt cgacgagaat gcggccattc
     1381 ggcgtgtcaa ggagggtctt gctcccggtg acggtgcccg cccctcccag gaatgtcagc
     1441 acaggcgaac tcgcagtcat cgcatccgac ccaaacgcct gttccagccc gacaggccct
     1501 cactgatgtg ggagttcacc attggcccga acccaccctc cttcgtcttc ccccgagcgt
     1561 atgcctcggc gccggcgccc gccacggacg aaagtcccga atccaggcga tcgtcgttga
     1621 cgagccgtcc cgccatgttc agcctttgac cacgccaagg ggtctgaggc gagccacctt
     1681 gctcgccagt cccgccgctt cacaaacgtc ggcaacggcg tcgacgtcct tgtaggcata
     1741 gggtgcctcc tcagagagca gtcgaacaga gcgcggtcgg accgaaattc ccgccgcctg
     1801 caacgacatc tgaacagatc gacccggcga gcgtctgctt gccttcttcc ggctcatcag
     1861 tctccctgcg ccgtgggcgg ccgaagcgaa agcagggttg ccctccacgc cacgaagcac
     1921 ccaggaagcg gtgcccatcg agcccggcac gagaacgggc tggccgtaat cggcgaaatc
     1981 cgccggaagg tcgggatgtc cgggtccgaa agctctcgtt gcccctttgc gatggacgca
     2041 cactcgggtg gcgcgtcctc cgacctcgtg gctctccatc ttggccaggt tgtgggccac
     2101 gtcgaagacg aggctcatgc cagtcgcttc cgggggaacc cccaaggccg cagcaaaacc
     2161 ctgtctcgct tcgtgggcca ggatctgtcg atttgcccag gcgaagttcg acgccgctgc
     2221 catggcaccc agataccgcc gcccctcgct ggagtcgatc gggacgcaag ccaactgacg
     2281 gtctggcacg tggattccgt gacgctgcat cgcctcaccc atcaatcgca attgatccgt
     2341 gcaggtctgg tgccccagtc ctctcgagcc acagtggatc atcacggtga gcatgcctgg
     2401 ggcgattccg aacgcttcgg ccgcctcggc atcagcga
//
LOCUS       DASUTA010000235         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_454939, whole genome shotgun sequence.
ACCESSION   DASUTA010000235 DASUTA010000000
VERSION     DASUTA010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454939"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="VFZ80_04710"
     CDS             complement(<1..934)
                     /locus_tag="VFZ80_04710"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX6286764.1"
                     /translation="MFTRVPSDYPTGRRRPVLVVLGILFAIYLLLTLAGTLWTDFLWF
                     ESVGFRDVWLRNWGLSILLGAIGVLFAFLVIWGALKLVDRFSPRWAPFDLTEEEELVE
                     RFREWVEPRIRQIRILITAGLAVIMGLTVATWRDDVFLFINSQGFGEVDAIFGVDLGF
                     YIFRLPLWETLVDWLFNLLVLTTVVVAIALYLNGAIRFDGRRVSATRGAKIYISSLFA
                     IIALVRAVSYRLDMFEILYSNRNEDFFGPGFTDITARLPVIRLLLIVALIAAVLFVVN
                     IFRRGWTLALVSVAAWIVVSIAAGTIYPAFVQRFS"
     gene            complement(1051..>2444)
                     /locus_tag="VFZ80_04715"
     CDS             complement(1051..>2444)
                     /locus_tag="VFZ80_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286765.1"
                     /translation="TMRERASLDAARWRSEAMEEADITRRQAAADAEAMRRDAWVSGS
                     ELLEQASANAEAMRAHAERDVLTIMGEAEREAHRLTSGARRESEDLIRNATMDAEKIT
                     GDATKRRDEIIDAANRQAAAAQERTRALEQRRDELLEELENVRSTLTRLEGSLDERRE
                     ALDLTAPSDSSSVRVVHPPAEAKKQWELGETVRVVPKDHPPRELPDDSLASEVSDEVA
                     RSQNAEATAEDETPTEPVAPPLPQPVAGPEPEPARSEAVDEGSSDDLSALFASLRDSD
                     EAQVPQSDQSPTEQDADEPESETEDETGGIDWIAVREERLLPITNRALRGVKKSMTEI
                     QNVALDSLRTDEEWTPDEDSIAESIHAELVAVWSESFAAGHAVAERMTDTKLKRPPTP
                     ASSAERGFAADLAAAVSGALEKAGDGPRERQSAASRVFRVWRSDEAERRIRDIALEAY
                     ETGIEESRKVATG"
BASE COUNT          446 a          785 c          774 g          439 t
ORIGIN      
        1 cgctgaagcg ctgcacgaaa gcggggtaga tcgtgccggc cgcgatcgaa accacgatcc
       61 aggcggcgac agagacgagg gccagtgtcc atcctcgtcg gaagatgttc acgacgaaca
      121 agaccgcggc gatcagggcg acgatgagca gcagacggat gaccggtaga cgggctgtga
      181 tgtcggtgaa accgggcccg aagaagtcct cgttgcggtt ggagtacagg atctcgaaca
      241 tgtccagccg ataagagacg gcccggacca gggcgatgat ggcgaacagg gacgagatgt
      301 agatcttggc tccacgtgtc gccgatacgc ggcgcccatc gaaccggata gctccgttga
      361 ggtagagggc tatggcgacg accacggtgg tcagcaccag caggttgaac agccaatcga
      421 ccaatgtctc ccacagcggg agacggaaga tgtagaagcc gaggtcgact ccgaagatcg
      481 catccacttc gccgaagccc tgcgagttga tgaacaagaa gacatcgtcc cgccatgtgg
      541 caacggtgag tcccatgatc actgcgagcc ctgccgttat caggattcgg atctggcgga
      601 ttcgtggttc cacccactcc cggaaacgct cgaccagctc ctcttcctcg gtcaggtcga
      661 acggcgccca gcgtggcgag aaacggtcga cgagcttgag ggcgccccat atgaccagga
      721 acgcaaagag gacacctatc gctccgagga ggatggacag cccccagttc cgcagccaaa
      781 cgtcacggaa gccgaccgac tcgaaccaga ggaagtcggt ccacaacgtc cctgccaggg
      841 tcaggagcag gtagatggcg aagaggatgc caaggactac caacaccggg cgacgccgtc
      901 cggtcggata gtcggaggga acccgggtga acatgagcgg cgaagtctaa gtggtgtgct
      961 atttaagcct gatcgtgtga ttcggcggcc gggcgcagga ttggaccgag agcgccgctg
     1021 agcgacgcca cgatccccgg ggggttgcag tcagccggtg gcgaccttcc tggactcctc
     1081 gatgccggtc tcgtaggcct cgagagcgat gtcgcgtatt cgcctctccg cctcgtcgct
     1141 cctccagacc ctgaagacac gtgaggctgc cgactgtctc tcccgagggc catcccccgc
     1201 cttttccagc gcaccggaca cggctgccgc caaatcggcg gcgaaaccgc gttctgcgct
     1261 cgaggcgggt gtaggcggcc tcttcagctt cgtgtcggtc attcgctccg caaccgcgtg
     1321 acctgcggcg aagctctccg accagactgc aaccagttcg gcgtggatcg attcggcgat
     1381 cgaatcctcg tcgggggtcc attcctcgtc ggtgcgaaga ctgtcgaggg ccacattctg
     1441 aatctctgtc atcgacttct tcactccacg caaggctcgg ttggttatcg gcaacagacg
     1501 ctcttcgcga accgcgatcc agtcgatgcc gccggtctcg tcctcggtct cggactcggg
     1561 ctcatcggca tcctgttcgg tcggcgactg gtcagattgg gggacttggg cctcatcgct
     1621 atcccgaaga gaggcgaaca gtgcagacag atcgtcagaa ctcccctcgt cgactgcttc
     1681 cgaccgggca ggttcgggtt ccggtccggc aacaggctga ggcaggggtg gagccaccgg
     1741 ctcggttggt gtctcgtcct cggccgtcgc ctccgcgttc tgcgaccggg ccacctcgtc
     1801 gctgacctcc gaggccaggg aatcatcggg caactccctg ggcggatggt ctttgggcac
     1861 gactcgcacg gtctccccga gctcccactg tttcttggcc tctgcgggcg gatgcacgac
     1921 gcgaacactc gaggaatccg atggagcggt gagatccagc gcctcccgtc gctcatcgag
     1981 cgacccctcc aagcgggtca gggtggagcg gacattctcc aactcctcga gcagttcgtc
     2041 acgcctctgc tccaaagccc gggtgcgctc ctgggccgcc gcggcctgcc gatttgcggc
     2101 gtcgatgatc tcgtcacgtc tcttcgtggc atcgccggtg atcttctctg cgtccatcgt
     2161 ggcgttgcgg atcagatctt cggactcgcg acgagccccg gaggtgaggc gatgcgcctc
     2221 acgctcggcc tcacccatga tcgtgaggac gtctcgctcg gcgtgggccc gcatcgcctc
     2281 ggcgttagct gaggcttgct ccagcaactc ggagcccgat acccaggcgt cacgacgcat
     2341 tgcctcggca tccgccgcgg cttggcgcct ggtgatgtct gcctcctcca tggcctcact
     2401 acgccaacgg gcggcatcga ggctggcccg ctcccgcatc gtct
//
LOCUS       DASUTA010000236         2811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_714993, whole genome shotgun sequence.
ACCESSION   DASUTA010000236 DASUTA010000000
VERSION     DASUTA010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714993"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..459)
                     /locus_tag="VFZ80_04720"
     CDS             complement(1..459)
                     /locus_tag="VFZ80_04720"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6286766.1"
                     /translation="MTTRLPPVNVSQHVTASPSEVLGFVSDTRNDSIWCPNVTGVTQI
                     DGDGVAVGSRFEFHQTVETGGRTLESDVEVEVVGLTENSVRWRVEDRFQIRDVTLTVE
                     PHGSGTKVRQQTIATFKQAPGLARWLYPLLARRTFRDQFSRLAEHFANEE"
     gene            complement(522..917)
                     /locus_tag="VFZ80_04725"
     CDS             complement(522..917)
                     /locus_tag="VFZ80_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286767.1"
                     /translation="MSLDDLNRRLAEIDAALDGEFSEASAEQYALLTERDRLRQVAAA
                     YWSSRDATRETEALEAELRELLRRRKAEVSTRSGYVMAKGGDNQGPASGAWVTLAAHS
                     RAASELSRLTTRIGEIESELERRRAANPS"
     gene            complement(935..1684)
                     /locus_tag="VFZ80_04730"
     CDS             complement(935..1684)
                     /locus_tag="VFZ80_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX6286768.1"
                     /translation="MAGHSKWANIKHRKGRQDAKRGKLFAKLAKAIEIAAREGGGSVE
                     FNASLATAVDKAKANDMPNDNIERAIKRGTGEVEGAAYEEMYYEGYGPGGVALYVHIL
                     TDNRNRAASDVRSTFTRHNSSLGEPGSVGYLFTQKGMILAKGAEDDVMLAALEAGAED
                     VRDGGDGMFEVVTQASDLRQVKEALTSAGLQVETADVTQLPSNLVPVEESEAKKLLRL
                     IDALDDLDDVQEVYSNYDIDDSVMEKALAEA"
     gene            complement(1695..2264)
                     /gene="pdxT"
                     /locus_tag="VFZ80_04735"
     CDS             complement(1695..2264)
                     /gene="pdxT"
                     /locus_tag="VFZ80_04735"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX6286769.1"
                     /translation="MTVKRVGVLALQGDVREHLHILESLGADAVGVKTPGQLAEVDGL
                     ILPGGESTTIGKLAARFGVLEPLRSAIDAGFPVYGTCAGMILLAGAVTEGDQPLVGAL
                     DVVVKRNAFGRQNESFEADLEIASLDRPFHAVFIRAPWIEKVGSEVEVLATVDDHPVM
                     VRQDDILATSFHPELTGDGRIHRMFLDSI"
     gene            complement(2261..>2811)
                     /locus_tag="VFZ80_04740"
     CDS             complement(2261..>2811)
                     /locus_tag="VFZ80_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX6286770.1"
                     /translation="EHHVDKWAFTTPFVCGARNLGEALRRIGEGAAMIRTKGEAGTGN
                     VVEAVRHMRTVTNEMRAVASMGDEQLMSAARDMGAPYELVREVAETGKLPVVNFAAGG
                     IATPADAALMMGLGVDGVFVGSGIFKSSDPQGYARAIVEATTFWNEPEVVAKVSRGLG
                     EAMPGLEMETLSESEQLARRGW"
BASE COUNT          465 a          948 c          859 g          539 t
ORIGIN      
        1 tcactcttcg ttcgcgaagt gctcggccaa ccgcgagaac tggtctcgga acgtgcgccg
       61 ggcgaggagc gggtagagcc atcgcgccag gcccggggcc tgcttgaagg tggcgatggt
      121 ctgctgtctc accttggtgc cgctgccatg cggctccacc gtgagtgtga cgtcccggat
      181 ctggaagcga tcctcgaccc gccaacgcac actgttctcg gtgaggccca ccacctcgac
      241 ttcgacatcg gattcgagcg ttcgtccacc ggtctccacg gtctggtgga attcaaaacg
      301 ggagcctacg gcgacgccat ccccatcgat ttgggtcacc cccgtcacat tcgggcacca
      361 gatggagtcg ttgcgggtgt cggacacgaa gccgaggacc tcagaagggc tcgccgtcac
      421 atgttgggag acattcaccg gtgggagccg tgtcgtcatt gcccggccgg gacgggatca
      481 tgagggtgga agcggcggga tgctcgatgc tccattgacc ctcagctcgg gttagcggcc
      541 cgtctccgct ccagttcgga ctcgatctca ccaatccttg tggtgagtcg gctgagttcg
      601 ctggcagctc gactatgtgc cgccaaggtc acccatgctc cggaagcggg gccctgattg
      661 tctccgcctt tggccatgac gtagccggaa cgggtcgaga cctcggcttt gcgccgccgc
      721 agcaactctc gcaactcagc ctcgagcgct tcagtctcac gggtggcgtc ccgtgacgac
      781 cagtaggcgg ccgcgacttg acggagccga tcgcgctcgg tcagcagggc atactgctcg
      841 gcagacgctt cactgaactc gccgtcgagg gcggcgtcga tctcggccaa gcgacgattc
      901 agatcgtcca gactcatgtc accctgcccg tccatcatgc ctcggcgagg gccttctcca
      961 tcacggagtc gtcgatgtcg tagttggaat agacctcctg gacgtcgtcg agatcgtcca
     1021 gggcatcgat gagccgcagc agcttcttgg cttcggattc ctccactgga accaagttcg
     1081 aggggagctg agtcacatca gcagtctcga cctgcaagcc ggcgctggtc agcgcctcct
     1141 tcacctgccg gagatccgac gcctgggtca ccacctcgaa catcccgtcg ccgccgtcgc
     1201 gcacgtcctc ggcacccgcc tcgagagcgg ccaacatgac gtcgtcctcc gcgcccttgg
     1261 ccaggatcat tcccttctga gtgaatagat agccgactga ccccggctca ccgaggctgc
     1321 tgttgtgtcg ggtgaaggtg gatctaacat cggaggcggc gcgattgcgg ttgtcggtga
     1381 ggatgtgaac gtagagcgcc actcccccgg gcccgtaccc ctcgtagtac atctcctcgt
     1441 aagccgcgcc ttcgacctcc ccggtccctc gcttgatcgc ccgctcgatg ttgtcgttgg
     1501 gcatgtcgtt ggccttggcc ttgtcgacag cggtggccaa ggaggcgttg aactcgacac
     1561 tcccgccgcc ctcacgtgcc gcgatctcga tcgcctttgc cagcttggcg aagagcttgc
     1621 cccgcttggc gtcctgacgc cctttgcggt gcttgatgtt cgcccatttg gaatgtccgg
     1681 ccatgactaa accctcagat cgagtcgaga aacatccggt gaatgcgccc atcaccggtc
     1741 agttccgggt gaaaggacgt cgccaggatg tcgtcctgac gcaccatcac cggatgatcg
     1801 tcgaccgtgg cgagtacctc tacctcagag cccaccttct cgatccaggg ggctcggatg
     1861 aagacggcgt ggaacgggcg gtcgagactc gcgatctcga ggtccgcctc gaacgactcg
     1921 ttctgacggc cgaacgcatt tctcttcaca accacgtcga gtgccccgac cagcggctgg
     1981 tctccctcgg tgactgcgcc ggccaagagg atcatccctg cgcatgtgcc atagacgggg
     2041 aacccagcgt cgatggctga gcgaagaggc tccagcacac cgaaacgggc ggccagtttg
     2101 ccgatggtgg tcgactcgcc tcccgggagg atcagcccgt cgacttcggc gagctgcccc
     2161 ggggtcttca cgcccacggc atcggctccg agcgattcga ggatgtgcag atgctcgcgc
     2221 acgtcgccct ggagtgccag gacaccgacg cgcttcaccg tcaccacccc cggcgggcga
     2281 gctgctccga ctccgaaagt gtctccatct cgagacccgg catggcttcg ccgagaccgc
     2341 gagagacctt ggccaccact tcaggctcat tccagaacgt ggttgcttcg acgatggctc
     2401 tggcgtaacc ctgcgggtcg gagctcttga agatccccga cccgacgaag acgccatcca
     2461 cgccgagccc catcatcagg gcggcgtcgg cgggcgtggc aatgccaccg gcggcgaagt
     2521 tgaccacggg aagcttcccg gtttcggcca cctccctgac cagttcgtag ggggcgccca
     2581 tgtcgcgtgc tgccgacatc agctgctcat cgcccatcga ggccaccgct cgcatctcgt
     2641 tggtgactgt gcgcatgtgg cgcactgcct cgacgacgtt cccggtgccg gcttcgcctt
     2701 tggtccggat catcgctgca ccttccccga tgcgacggag cgcctcaccg agattgcgcg
     2761 ctccgcacac gaacggcgtg gtgaaggccc acttgtcgac gtggtgctcc t
//
LOCUS       DASUTA010000237         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_457031, whole genome shotgun sequence.
ACCESSION   DASUTA010000237 DASUTA010000000
VERSION     DASUTA010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457031"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..515
                     /locus_tag="VFZ80_04745"
     CDS             204..515
                     /locus_tag="VFZ80_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX6286771.1"
                     /translation="MAHTLISAGSDGVYIDVHVQPRARRPGVRGIHGDRLKIAVTEPP
                     EGGRANKATVRLIARALEVKPGDVSLVAGARSRDKRLFVAGLNGHLAQRLIEKAIEGS
                     A"
     gene            559..1023
                     /locus_tag="VFZ80_04750"
     CDS             559..1023
                     /locus_tag="VFZ80_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286772.1"
                     /translation="MAPKDDSKREESSINLSWLTTGIFTIAAMMGLWWAQVQQESIQQ
                     AASTLEQPAGLWMLWSLTLVAVGICFGLAVASGSGWRRRIPFTALLWAGVPLAIVLAF
                     HLWVGQIVDLPIEFLRVLVGIQMQVASAVAVGVFLSALLSPVLPPPHSPGGA"
     gene            complement(968..2251)
                     /locus_tag="VFZ80_04755"
     CDS             complement(968..2251)
                     /locus_tag="VFZ80_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX6286773.1"
                     /translation="MAVETLDSGRPDPDVDITSHDTYVEDFPHSAFRRLRRDDPVSWV
                     TESDGSGFWAVTKHEDVVEVSRDYNRFTASRGIRIEEMDHEELEARRSMMEFDPPEHT
                     RLRRLVQPGFTPKVTATYEEAFRRLVGVVLDRVLPLGEFDFVTEIARELPIRMLCRLL
                     GIPEEDAGQMVAWGDQMISNADPEYTPVIIDKVDTAEYRLLPFRSPAALEVFRYAEEI
                     ALERRRDPRDDIITTLLTAEPDGEPLTDLEFKNFFTLMMVAGNETTRHTISHGLVYLS
                     QAPDQLAWWRDDLVGRSPAATDEILRASAVTMHFRRTVAEDTEVRGVPMSIGDRVVVW
                     YTSANYDDDVFAEPFSFDLSRKPNPHLTFGTGRHKCLGASLAELEVRVFFEEFLHRVD
                     SFQVGEPERLRSNFIRGIKHLPVSVAEATPGSAVH"
BASE COUNT          407 a          730 c          732 g          432 t
ORIGIN      
        1 tcgagaggac tctggcgtct cattgaagaa cccgccgcca tgggtgcctc tggcgggagg
       61 tgggactcga gaaaccgccg ctcgatgtca cccaacaggg ggatgaccgt atttgcgtcc
      121 cggtcgtcct tggtcgtcac gacgagaacc tatagctcga aatcgtcggg cgcgggttca
      181 cctcctctcc tacggtcgcc accatggcac acaccttgat cagcgccggc tccgacgggg
      241 tctacatcga cgtacacgtc caaccccgag ctcgtcggcc gggggtgcgc ggcatccacg
      301 gcgaccgtct gaagatcgcg gtcaccgaac caccggaggg cggaagggcc aacaaagcca
      361 cagtcaggtt gatcgcccgc gccctcgagg tgaaaccggg agacgtctcc ctggtggcgg
      421 gagctcgatc tcgtgacaag cggcttttcg tggccggact gaacggccat ctggctcagc
      481 gcctgatcga aaaggcgatc gaggggtcgg cctgacaaag acgaagagcc gggacggccc
      541 actcctactc tgggcatcat ggcccccaaa gacgactcca agcgggagga gagctcgatc
      601 aacctgagct ggctcaccac cgggatcttc accatcgcgg cgatgatggg actgtggtgg
      661 gcacaggttc agcaggagtc gatccagcag gccgcctcca ccctcgaaca gccggccgga
      721 ctttggatgt tgtggtcact gacgctggtc gctgtcggca tttgcttcgg tctcgccgta
      781 gcttcgggct cgggttggcg ccgacgcatc ccgttcacgg cactcctttg ggccggggtc
      841 ccgctggcga tcgtcctcgc cttccacctc tgggtcggcc agatcgtgga tctgccgatc
      901 gagttcctcc gggtgctcgt cggaatccag atgcaggtgg cgtccgcggt cgcagtcggc
      961 gtctttctca gtgcactgct gagcccggtg ttgcctccgc cacactcacc gggaggtgct
     1021 tgatcccccg gatgaagttg gagcggagtc gctccggctc gccgacctgg aacgaatcga
     1081 ccctgtgcag gaactcttcg aagaagaccc tcacctccaa ctctgccagg gacgcgccta
     1141 ggcacttgtg ccgcccggtg ccgaacgtga ggtgagggtt gggcttgcgt gacaaatcga
     1201 acgagaaggg ctcggcgaag acgtcgtcgt cgtagttggc tgacgtgtac cacacgacca
     1261 cccggtctcc tatcgacatc ggcacgcccc gtacctcggt gtcctcggcg acagtccgcc
     1321 ggaaatgcat cgtcaccgcc gaggcgcgaa ggatctcatc ggtggctgcc ggcgatcgtc
     1381 ccacgaggtc gtcgcgccac caagccagct gatcgggtgc ctggctcagg tagacgaggc
     1441 catggctgat ggtgtgtcgc gtcgtctcgt tgcccgccac catcatcagc gtgaagaagt
     1501 tcttgaactc gaggtcggtg aggggctcac cgtcgggctc ggcggtgagc agcgtggtga
     1561 tgatgtcgtc acggggatcg cggcgacgct ccaaggcgat ctcctcggca tacctgaata
     1621 cctcgagtgc cgccggtgag cgaaacggga gcaaccggta ctccgcggtg tcgaccttgt
     1681 cgatgatcac cggggtgtac tccgggtcgg cgtttgagat catctgatca ccccaagcca
     1741 ccatctgccc ggcgtcctcc tcggggatcc ccagcaaccg gcagagcatt cgtatcggca
     1801 gctcgcgagc aatctcggtc acgaagtcga actcgcccaa aggaagcacg cggtcgagga
     1861 cgacaccgac caatctgcgg aacgcttctt cgtaggtggc ggtcaccttt ggggtgaatc
     1921 cgggctggac cagccttcgc aggcgggtgt gctccggtgg gtcgaattcc atcatcgacc
     1981 tgcgggcctc cagctcttca tggtccatct cctcgatcct gatgcctcgg gaagcagtga
     2041 accggttgta gtcgcggctc acttcgacga cgtcttcgtg ctttgtcacc gcccagaacc
     2101 cgctcccgtc gctctcggtg acccacgaga cagggtcatc gcggcgaagc cggcggaatg
     2161 cggaatgggg gaagtcctcg acgtaggtgt cgtgagacgt gatgtcgaca tctgggtcgg
     2221 gtcttcctga atcgagtgtc tcaaccgcca tttcgcacac cgaacataat cccgggcgaa
     2281 ctcattcggg cacgtcggga a
//
LOCUS       DASUTA010000238         3604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1106789, whole genome shotgun sequence.
ACCESSION   DASUTA010000238 DASUTA010000000
VERSION     DASUTA010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106789"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1606)
                     /locus_tag="VFZ80_04760"
     CDS             complement(113..1606)
                     /locus_tag="VFZ80_04760"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX6286774.1"
                     /translation="MKNIHHWVDGSFTAGSSTRSGPVFNPATGQQTGEVAFASGEDVD
                     LAVRAAAEAYESWRFSSVTQRQNIMFAFRELVAARRREIAEVLTSEHGKTIDDALGEV
                     QRGLEVVEFACNIAHLLKGDYSEQVSSGVDTYTIRQPIGVVAGITPFNFPAMVPMWMY
                     PIAIACGNTFVLKPSEKDPSTSQLAAELLAEAGLPDGVFNVVHGDKEAVDALITHPDV
                     AAISFVGSTPIARYIYETGTQNGKRVQALGGAKNHMIVLPDADMDLAADAAVSAGYGS
                     AGERCMAISAVVAVGDAGDRLIDGVQQRIEKLNVGPGDQPGVEMGPLVTRQHLDRVRG
                     YVDAGESEGALLVADGRNFVVDGYEDGFFLGPTLFDNVRPDMSIYTDEIFGPVLSTVR
                     MEHYEEAIQLINDNPYGNGTAVFTNDGGAARKFQQEIQVGMVGINVPIPVPLSFYSFG
                     GWKNSIFGTHAIYGPEGVHFYSRQKVVISRWADPTHRGVDLGFPTHG"
     gene            1651..2037
                     /locus_tag="VFZ80_04765"
     CDS             1651..2037
                     /locus_tag="VFZ80_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286775.1"
                     /translation="MTAQEAALSAALLLGWVALVHLLMASGLRLGELVWSGQQPRLLE
                     PGLRVRSFLFGIGVAASGLVLLFATGVLDSPIPPRWDRSATFAVSAFLGVGFLYCLFA
                     GSRWERMLFAPVLLLGSALAGWLTFA"
     gene            complement(2049..2387)
                     /locus_tag="VFZ80_04770"
     CDS             complement(2049..2387)
                     /locus_tag="VFZ80_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286776.1"
                     /translation="MGWCLHSATITTITAGLAREVCETCGRVRIHYVERAVQIYPTPP
                     RLMVESLRQPSETLSSPRNRTCMLCSQSAVFMIPDGAVCEEHAWQAAARTSWDDAEVW
                     VPIRIDKSNA"
     gene            2843..3361
                     /locus_tag="VFZ80_04775"
     CDS             2843..3361
                     /locus_tag="VFZ80_04775"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX6286777.1"
                     /translation="MTQISTSLTDITTVEVDAIVNAANSRLAGGGGVDGAIHAAGGPS
                     ILAECRAWVAENGLLATGESMITTGGDLPARYVIHTVGPIYADHEPEEGERLLAGCYR
                     NSLDLGAHHSIESIAFPNISTGVYGYPKADAAATAVAAVKEWVEVNDGVDEVTFVCFD
                     EANHRIYSELLA"
     gene            complement(3340..3543)
                     /locus_tag="VFZ80_04780"
     CDS             complement(3340..3543)
                     /locus_tag="VFZ80_04780"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HEX6286778.1"
                     /translation="MPTGQPDSVETHADRLVREAMEAGQFDDLPGAGEPLPGAGTKDD
                     AGWWIRGWLARNRATPDQASNSE"
BASE COUNT          638 a         1126 c         1142 g          698 t
ORIGIN      
        1 ctcgtcttct cccatcggag cggcgagatt aggtgggctc gatcaaccgt gggtggggaa
       61 acccaggacg cctccccctc tgggggaggt ttggagtcct ccggaactcc cttcacccat
      121 gtgtcgggaa accaaggtcc actccgcggt gggtggggtc ggcccatcgt gagatgacaa
      181 ccttctggcg cgagtagaag tggacgccct cggggccata gatggcatga gtcccgaaga
      241 tcgaattctt ccaaccgccg aagctgtaga aggacagcgg gaccggtatg gggacgttga
      301 tccccaccat gcccacctgg atctcctgtt ggaactttcg ggccgcccct ccgtcgttgg
      361 tgaagacggc cgtgccgttc ccgtatgggt tgtcgttgat caactggatg gcttcctcgt
      421 aatgctccat gcggaccgtc gacagaaccg gtccgaagat ctcgtcggtg tagatcgaca
      481 tgtccggtct cacgttgtcg aagagggtgg gcccgaggaa gaaaccgtcc tcgtagccgt
      541 caaccacgaa attgcgcccg tcggcgacga gcagcgcgcc ctccgactcc cccgcatcga
      601 cataccccct cacccggtcc aggtgctgac gggtgaccag cgggcccatc tcgacacccg
      661 gctgatctcc cgggccgaca ttgagcttct cgatacgttg ttgaacgccg tcgatcagac
      721 ggtcgccggc atcgccgacc gctacgactg cggatatcgc catgcaccgc tccccggccg
      781 agccgtagcc ggcgctgact gcagcatcgg cagcaagatc catgtcggcg tctggaagga
      841 cgatcatgtg gttcttggcg ccgcccagcg cctggacccg cttcccgttc tgtgtccccg
      901 tctcatagat gtaccgggcg atcggtgttg atcccacgaa agagatcgca gccacatcgg
      961 ggtgagtgat gagggcgtcg acggcctcct tgtccccatg gaccacgttg aacacaccat
     1021 cgggaaggcc ggcttcggct agcaactcgg cggccagttg tgaggtcgaa gggtccttct
     1081 ccgaaggttt gaggacgaag gtgttgccac aggcgatggc gattgggtac atccacattg
     1141 gaaccatcgc cgggaagttg aacggcgtga tcccggccac aaccccgatc ggctgacgga
     1201 tcgtgtaggt atcgacgccc gacgacacct gctcggagta atcgcctttc agcaggtgag
     1261 cgatgttgca ggcgaactcg accacctcga gccctcgctg gacctcgccg agggcgtcat
     1321 cgatggtctt gccatgctcg gaggtgagga cttcggcgat ctcccgacgg cgagccgcca
     1381 ccagctcgcg aaaagcgaac atgatgttct ggcgctgggt gacggatgag aacctccagc
     1441 tctcataggc ttctgccgca gcacgaaccg cgaggtcgac gtcttcgcca gaggcgaatg
     1501 ccacctcgcc ggtctgctgg ccggtcgcgg gattgaagac cggaccgctg cgcgtcgacg
     1561 agccggcggt gaatgagccg tcaacccaat ggtggatgtt cttcatgctc tgaacggtag
     1621 cgaggcagct tcagtgtaag ttggctgacg atgactgccc aagaagccgc cttgagcgca
     1681 gcgttgctgt tgggatgggt cgccctggtg catcttctga tggcatcggg tcttcgactc
     1741 ggcgagctcg tttggtcggg gcagcaacca aggctcttgg agcccgggct cagggttcgg
     1801 tcctttctgt ttggcatcgg cgtcgcagca tcgggattgg tgcttctctt cgccaccggg
     1861 gtgctcgact caccaatacc ccccagatgg gaccgatcgg ccacgttcgc ggtgtcggcg
     1921 ttcctgggag ttggttttct ctactgcctg ttcgccggtt cccgctggga gcggatgctc
     1981 ttcgccccgg tcctgctgct cggctcggcg ctggccggct ggctcacctt cgcttagacg
     2041 agccgtcgct aggcgttgga cttgtcgatg cggatcggga cccaaacctc ggcgtcgtcc
     2101 cagctggtgc gtgcggctgc ctgccatgcg tgctcctcgc agacggcgcc atcagggatc
     2161 atgaagaccg ccgattgact gcacagcatg catgtgcggt tgcgcggcga actgagggtc
     2221 tcgctgggtt ggcggaggga ctcgaccatc agtcggggcg gcgtcggata gatctggaca
     2281 gctcgctcca cgtaatggat tcgaactcga ccgcacgtct cgcagacttc gcgggcgagc
     2341 ccggccgtga tcgtcgtgat ggtggcgctg tgcaggcacc atcccaagtt ccgccacttg
     2401 ttcaacctgc tgcctcccaa caggaaaccc ggtcttcaaa ctagccatcg tctgccccgc
     2461 gtgccagagg aacttcggac ttgtgcaaag ggcactagtc cgaccatttc tgggtctgcc
     2521 gcctcatgtc gcggcagcaa gtgcgtgaga gaatctccgg tcaaggtcaa ttcatctttc
     2581 tcgccaggga ggcaccgaaa gtgttaggca gaagaggcag attcgctcgc cccgattgcg
     2641 agcacctcga caccgtcacc gttcgcaaca acgggatcga acgaaccgtt tgtgagacct
     2701 gcggacatgt cagtttcaag ggcttggaaa gcctgtcagg gaaggcaagc cggagtcagt
     2761 tcgagcgggc gtcagagcgc gcctcgaccg tgaccggcta ggttcggatc gacgtcggta
     2821 actcccgacg tcgatccgtc cgatgacgca gatctccaca tcgctcacag acatcaccac
     2881 cgtcgaggtc gacgcaatcg tcaacgctgc caactcacgt ctggcgggtg gtggcggagt
     2941 cgacggagcc attcacgccg cgggcggccc atcgatcctt gcggagtgcc gggcctgggt
     3001 ggcggagaac ggtctccttg ccacgggcga atccatgatc acgacgggag gcgatcttcc
     3061 ggcccgttat gtgatccaca ccgtcgggcc gatatacgcc gaccatgagc cggaggaggg
     3121 ggagcggcta ctggctggct gttatcgaaa cagcctcgac ctcggggcac accactcgat
     3181 cgagtcgatc gctttcccca acatctcgac cggggtgtac ggatacccca aggccgacgc
     3241 cgcagccacc gccgtcgccg cagtcaagga gtgggtcgag gtcaacgatg gtgtcgacga
     3301 agtgaccttc gtttgcttcg acgaggcaaa ccatcggatc tactcggagt tgctcgcctg
     3361 atcaggagtc gcgcgatttc tcgcgagcca tcctcttatc caccagccgg cgtcgtcctt
     3421 ggtgcctgcc cctgggaggg gctcgccggc accggggaga tcatcgaact ggcccgcctc
     3481 catcgcctcg cgtacgagac gatcggcatg ggtttcgacg gaatcgggtt ggccggtagg
     3541 catggaggaa ggaccctacc ggccgttccc gactcactag gccgccggca gggtcctacc
     3601 ggcc
//
LOCUS       DASUTA010000239         6103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_846662, whole genome shotgun sequence.
ACCESSION   DASUTA010000239 DASUTA010000000
VERSION     DASUTA010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6103
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846662"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VFZ80_04785"
     CDS             <1..883
                     /locus_tag="VFZ80_04785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6286779.1"
                     /translation="RPEDDAFWEDPPSQITSAVYTPIAGYITDPQLATHNLQRAAEAK
                     GAEFLFNTAVTGIRVEDGRVAGVEIEDGTPIDAPIVVNVAGPHSFVINEMAGVADTMA
                     IKTRALRHEVHHVPSPEGLDFEKDGMLVGDDDLGTYFRPEVANHILVGSQDPDCDPQI
                     WVEDPDEFDRAITEDQWRAQVLRLARRLPNLGVPNEKKGVVDLYDVSDDWIPIYDKTD
                     LPGFYVAIGTSGNQFKNSHIASYCLTKLIQAVEAGQDHDTDPVEVNMPYTGLTIDMSA
                     FSRNREINRASSFSVLG"
     gene            complement(867..1994)
                     /locus_tag="VFZ80_04790"
     CDS             complement(867..1994)
                     /locus_tag="VFZ80_04790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6286780.1"
                     /translation="MTLPARVIQAVRPHASPGVMAGAAVVAATFAATPFLLPDLSTRM
                     GMDIGNTGFLSTAQVGSFAFSTFLAGRLLRPRRRLHYGSLGLIALATAASALVTTFPL
                     LLVTRVVAGLGMGTLTWIAWADATRSPRGMGDVAAIAPVTATIASPVLGWITGAGGYP
                     WVFGSLAALALLAALAPVDFGDLPRIGRNVSGSRSNRLLLIGLLVLSVGGSSVFIFTG
                     AAAEAVHGLSPVTVSWALSLNAICGVIATRRNATRGTAALWIGLTAASAIAVGVIASP
                     IVFFIALAVWGFAFWMAIPEVLKMLTERSLSPSERMGDAQAAMAVGRVFGPVVGAFAL
                     GAGLYTRLSLVGAALMTCAAVVIGAVELYRLRSSEPVSRGR"
     gene            2021..4330
                     /locus_tag="VFZ80_04795"
     CDS             2021..4330
                     /locus_tag="VFZ80_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HEX6286781.1"
                     /translation="MRIVTIGGGPAGLYLAILLKKADPAIEIEVHERNGPDDAYGFGV
                     VFSDETLDHFAAADEESFHGLTESFVRWGDIKVHHFSGMEVVSGGHGFAAASRRNLLH
                     ILSNRAVELGAEIRWFSDVSDVTRFEDADLVVGADGANSTVRRSLAHVLRPTVGVRTN
                     KYIWFGTPKVFDEFNFIFVDTAAGMVWAHIYPYSEEGSTFIAEMAPATWHALGFDVTE
                     NDELPLGVSDELALKQCEELFREHLDGMGLVGNNSKWLQFPTISCERWHADDVVLMGD
                     AVHTAHFSVGSGTKLAMEDAISLSRHVLAGVSLEDALTAFQEERRPEVDSLQRAAKAS
                     LEWFEGADRYRDMEPEQFVFSMLTRSQRVTYDNLRLRDSRYMESIDRWYAASDHGSPV
                     AVDPRVPPMFHPYRVRELTLPNRVVVSPMDQYSAVDGVPTTWHLVHLGSRAVGGAGLV
                     MTEMTCVSAEGRISPGDTGIWNDAQTRAWGEIVEFVHGHTEAKIGLQLGHSGRKGATK
                     LMWEGDNVPLEAGEWDIISASPLKYRPDSQVPKEATLADLATIKDQHVEAAKRGDAID
                     FDWLELHFAHGYFLSSFLSPLTNQRSDHYGGNLENRMRYPLEVFDSVRAVWPEGKPMS
                     VRFSATDWVEGGFDGDDAVVFARVLKDHGCDLIDVSTGQTTIHARPEYGRLYQTPYSD
                     RIRHEAGIATMTVGAVSSIDDIHNILVAGRADLVVMARPHLVDPYWTMNAAIDLGYTA
                     HPFPNQYLSGLGARRREQDPVAPEVFRQG"
     gene            complement(4339..5430)
                     /locus_tag="VFZ80_04800"
     CDS             complement(4339..5430)
                     /locus_tag="VFZ80_04800"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX6286782.1"
                     /translation="MTALAVLTSGGDAPGMNAAIRAVTKVAASHGVPTWGVENGYDGL
                     IDGSFRPLVRPNRVGIPTPVEEVENEGGEGGTILGSSRSKRFFERKSRVEATRQLQEH
                     SITAMIVIGGNGSMAGIHALAEETDVPIVGIPASIDNDIGLTREALGVDSALNTIVEA
                     CDNISDTARSHHRAFIVEVMGRQSGYLAMAAAVATAADAVLMPERPHTRDEVIDSVVR
                     VIETSFDAARDKTRVLIIKAEGVPVPTHELVEEATRRLTGGPREYEIRGAVLGHVVRG
                     GDASFRDRLLAGRFGRVAVEAALDGESDVMTGWNIGVELGKPTTDDWIRLFDISDVLE
                     ETKALLEGTSSVSLDRVSRMEAIQGVLAL"
     gene            complement(5465..6010)
                     /locus_tag="VFZ80_04805"
     CDS             complement(5465..6010)
                     /locus_tag="VFZ80_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX6286783.1"
                     /translation="MTYVALLRGINVGGQRKVEMGRLKAAFERAGASDVRTYINSGNV
                     IFDDDRSVADLVPILEKAIENEFDFHVKVLLRNRDAIAAVVEAIPEHWVNDKTERTDV
                     WFLWEEYDTPEVIDELAIEEGIDEVFYVDGAVVWRADGSQLTRSGRAKVIGSDLYKHL
                     TARNVNTLRKIHQLMLETSNG"
BASE COUNT         1142 a         1973 c         1934 g         1054 t
ORIGIN      
        1 gcgacccgag gacgacgcct tctgggagga cccgcccagt cagatcacca gcgccgtata
       61 cacgccgatc gccggctaca tcaccgatcc ccagctcgcc acccacaacc tccagcgcgc
      121 cgccgaagcg aagggcgccg agttcctgtt caacaccgcg gtgaccggga tcagggtcga
      181 ggacggaaga gtggccggcg tcgagatcga ggacgggaca ccgatcgatg ccccgatcgt
      241 ggtgaatgtc gccggaccac attccttcgt catcaacgag atggccggcg tcgccgacac
      301 catggcgatc aagacgaggg cgctgcgtca tgaggtccac catgtgccct caccggaagg
      361 actcgacttc gagaaggacg ggatgctggt gggtgacgac gatctcggca cctacttccg
      421 ccctgaagtg gccaaccaca tccttgtggg gagtcaggac ccagattgcg acccgcagat
      481 ctgggtggag gaccccgatg agttcgatcg ggccatcacc gaggatcagt ggcgggcaca
      541 agtgcttcga ctcgcccggc ggcttcccaa cctcggggtg cccaacgaga agaagggcgt
      601 cgtcgacttg tatgacgtca gcgacgactg gatcccgatc tatgacaaga ccgacctacc
      661 cgggttctac gtcgccatcg gcaccagcgg caaccagttc aagaactcgc acatcgcctc
      721 ctattgcctg accaagctga tacaggccgt ggaggccggt caagaccacg acaccgaccc
      781 ggtcgaggtg aacatgccct ataccggtct caccatcgac atgagcgcgt tcagtcggaa
      841 tcgggagatc aacagggcat cctcgttcag cgtcctcggc tgactggctc ggagcttcgt
      901 agccgataga gctccacggc tccgatgacg actgcggcac atgtcatcag cgccgcgccg
      961 accagcgaga gacgcgtgta caagcccgcg ccgagggcaa aggcacccac caccgggccg
     1021 aagacacgac ccacagccat cgccgcctga gcgtcgccca tgcgctcgct cggggagagg
     1081 gagcgttcgg tgagcatctt cagcacctcg gggatggcca tccagaaggc gaatccccac
     1141 acggccagcg cgatgaagaa gacgatcggc gaggcgatga ctcccaccgc gatcgccgac
     1201 gccgcagtga gcccgatcca gagagctgcc gtcccccgtg tggcgttgcg tctcgtggcg
     1261 atcactccac aaatcgcgtt gaggctcagc gcccatgaca cggtgaccgg gctcaggcca
     1321 tggaccgcct cggcggcggc gccagtgaag atgaacaccg aggagccccc gaccgacaag
     1381 accagaagtc cgatgagcag gagacgattg ctccgcgatc cgctcacatt gcgcccgatg
     1441 cggggcaggt ctccgaagtc gacgggcgcg agggcggcga gcaaggcgag ggcagcgagt
     1501 gaaccgaaga cccacggata acccccggcc ccggtgatcc atcccagcac aggcgaggcg
     1561 atcgtggccg tgacgggagc aatcgcagcc acgtctccca tgccccgtgg cgagcgggtg
     1621 gcgtcggccc aggcgatcca cgtgagggtg cccatcccca gcccggccac gacccgggtc
     1681 acgagcagga gtggaaacgt ggtcacgagc gccgaggcgg ccgtggccaa cgcgatcagc
     1741 cccagcgacc cgtagtgcaa gcgtcgcctc ggtctcagga gacggccggc aaggaacgtt
     1801 gagaaggcga aactgcccac ctgagccgtg ctgaggaatc cggtgttgcc gatatccatg
     1861 cccatccggg tcgacaggtc ggggaggaga aagggcgtgg cggcgaaggt ggcggccacg
     1921 actgccgctc cagccatcac accaggtgaa gcgtgagggc ggaccgcctg gatgacgcgg
     1981 gcggggagtg tcatgaccgc ggcagtatgg ttcgccgccg atgagaatcg tgaccatcgg
     2041 tggagggccg gcgggtctct atctggcgat cctgctcaag aaggcagatc cggccatcga
     2101 gatcgaggtg catgagcgca atggccctga cgacgcctat gggttcggtg tcgtgttctc
     2161 cgatgagact ctcgaccact tcgccgccgc cgacgaggag tcgtttcacg ggctcaccga
     2221 gagcttcgtc cgctggggcg acatcaaggt ccatcacttc tcgggcatgg aggtcgtctc
     2281 cgggggccac ggcttcgctg cagccagtcg tcgaaacctc cttcacatcc tgtcgaaccg
     2341 ggctgtggag ctcggcgctg agatccgttg gttctccgac gtgtccgatg tcactcgctt
     2401 cgaagacgca gacctggtcg tgggcgccga tggggccaac tcgacggtgc gaaggtcttt
     2461 ggctcatgtt ctccggccca cggtgggcgt gcggaccaac aagtacatct ggttcgggac
     2521 tccgaaggtc ttcgacgagt tcaacttcat cttcgtcgac accgccgccg gcatggtctg
     2581 ggcccatatc tacccatatt ccgaggaggg gagcacgttc atcgccgaga tggctcccgc
     2641 cacctggcat gccctcggtt tcgacgtcac cgagaacgat gagcttcccc tcggcgtcag
     2701 cgacgagctc gccttgaagc aatgcgagga gctctttcgt gagcacctcg acggcatggg
     2761 tctggtggga aacaactcga agtggctgca gttcccgacg atcagttgcg agcggtggca
     2821 tgccgatgat gtcgttctca tgggcgacgc cgttcacacc gctcacttct cggtcggctc
     2881 cggaaccaag ctggcaatgg aggacgccat ctccctctct cggcatgtcc tcgccggggt
     2941 ctctctggag gacgccttga ccgcctttca ggaggagcgc aggcccgagg tggatagcct
     3001 gcaacgggcg gccaaagcca gtctggagtg gttcgaaggt gcggatcgct atcgagacat
     3061 ggagccggag cagttcgtct tcagcatgtt gacccgaagc cagcgggtca cctacgacaa
     3121 cttgaggctt cgcgactccc gatacatgga gtcgatcgac cggtggtacg ccgcatccga
     3181 tcacgggtcg ccggtggcgg tcgacccgag agtgccgccg atgttccacc cgtatcgggt
     3241 gcgtgagctc actttgccca accgtgtcgt ggtctccccg atggaccagt actcggccgt
     3301 cgacggtgtt cccaccacgt ggcacctggt gcacctgggg agccgggccg taggtggagc
     3361 gggactggtg atgaccgaga tgacctgtgt ctcggccgaa ggtcggatct ctcctggtga
     3421 caccgggatc tggaacgacg ctcaaactcg agcctggggt gagatagtgg agttcgtcca
     3481 cggtcacaca gaggcgaaga tcggtctcca gctcggacat tccgggcgca aaggggctac
     3541 caagctgatg tgggagggtg acaacgtccc gctggaggca ggggagtggg acatcatctc
     3601 cgcgtcacct ctcaagtacc ggcccgacag ccaggtcccc aaggaggcca cccttgccga
     3661 cctggccacg atcaaggatc aacacgtgga agcggcgaag cgaggcgacg ccatcgactt
     3721 cgactggctc gagctccact tcgcccacgg gtacttcctc tccagtttcc tgagccctct
     3781 gaccaatcaa cgctccgacc attacggagg caacctcgag aacaggatga gataccccct
     3841 cgaggtcttc gactcggttc gggccgtctg gccggagggc aaaccgatgt cggttcgctt
     3901 ctcggcgacg gactgggtcg agggcgggtt cgacggcgac gacgcagtcg tgttcgcccg
     3961 agtcttgaaa gaccacggct gtgatctgat cgatgtctcc accggccaga ccaccatcca
     4021 cgcccggccc gagtacggaa ggctgtacca gaccccgtac agcgaccgca tcaggcacga
     4081 ggcgggcata gccactatga cggtcggtgc ggtctcctcc atcgacgaca tccacaacat
     4141 cctcgtggcg ggccgagccg acctcgtcgt catggcccgg ccccacctcg tcgacccgta
     4201 ctggacgatg aacgccgcca tcgacctcgg ttacaccgcc cacccgttcc ccaatcagta
     4261 cctgtccggg ttgggggcga gacggaggga gcaggacccg gtggcaccgg aggtgttccg
     4321 gcagggctga aacactccct acagggcgag cacgccctgg atcgcctcca tccgggagac
     4381 acgatcgagg gagaccgaag acgtgccctc cagcaatgcc ttcgtctcct cgaggacgtc
     4441 ggagatgtcg aagagcctga tccagtcgtc ggtggtgggc tttcccagct ccacaccgat
     4501 gttccatcca gtcatcacgt cactctcacc gtcgagagca gcctccacgg cgacacgccc
     4561 gaaacgaccg gcgaggagcc ggtcccggaa ggaggcatcg ccaccgcgaa cgacgtggcc
     4621 gagcactgcc cccctgatct cgtactccct gggcccgccg gtcagacggc gagtcgcctc
     4681 ttcgacgagc tcgtgggtcg gcaccggcac accttcggct ttgatgatga ggacccgggt
     4741 cttgtcacgg gcggcgtcga agctggtctc gatgactctg accactgagt cgatcacctc
     4801 atcacgtgta tggggtctct ccggcatcaa gaccgcgtcg gcggccgtgg ccacggcggc
     4861 ggccatcgcc agatagccgg attggcgccc catcacctcg acgatgaagg cgcgatggtg
     4921 ggagcgtgcg gtgtcggaga tgttgtcgca ggcctccacg atggtgttca aggcgctgtc
     4981 gacgcccaga gcctctcggg tgagcccgat gtcgttgtcg atggacgccg gtatgcccac
     5041 gatcggcaca tcggtctcct cggcgagcgc atggattcca gccatggagc cgttgcctcc
     5101 gatgacgatc atcgccgtga tcgagtgctc ctggagttgc cgtgtcgcct cgacacggga
     5161 ttttcgctcg aagaagcgct tgctccggct ggacccgaga atggtcccac cctcaccccc
     5221 ctcgttctcg acctcctcca ctggggtcgg gatgccgacc cgattgggtc tgaccagagg
     5281 gcgaaatgag ccgtcgatca gcccgtcata tccgttttca acgccccagg tggggacccc
     5341 gtgtgaggcg gcgaccttgg tgacagcacg aattgccgcg ttcatgccgg gggcgtcacc
     5401 tccggacgtg agcacagcca gcgcggtcat gacgaaacgg taactcgcgc aggcgaatcc
     5461 gtcttcatcc gttcgatgtt tcgagcatca gctggtggat cttgcgcagt gtgttgacgt
     5521 tccgagcggt cagatgcttg tagagatccg acccgatcac cttggcccgg ccactcctgg
     5581 tcagctggga gccgtcggcg cgccagacaa cggctccgtc cacatagaac acctcgtcga
     5641 tgccttcttc gatcgccagc tcgtcgatca cctcgggtgt gtcgtactcc tcccagagaa
     5701 accagacatc ggttcgctct gtcttgtcgt tgacccagtg ttcggggatg gcctcgacca
     5761 ccgcggctat ggcatcccga ttgcgaagca ggaccttgac gtggaagtcg aattcgttct
     5821 cgatggcctt ctccaggatg ggaaccaggt cggcgacgct ccgatcgtcg tcgaagatca
     5881 cgtttcccga gttgatgtat gtcctgacgt cgctggcgcc ggcgcgttcg aaagccgctt
     5941 tgagacggcc catctcgacc ttcctctggc ctccgacgtt tatcccgcgc agtagcgcta
     6001 cgtaggtcat ctcactcacc gcgtcctcaa ggtaggtggt gtgtccacat tgcggcaacc
     6061 aaaggtagaa tcgccgaagc cggcagggtg ccgtgcaggg ttc
//
LOCUS       DASUTA010000240         3897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_847078, whole genome shotgun sequence.
ACCESSION   DASUTA010000240 DASUTA010000000
VERSION     DASUTA010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847078"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VFZ80_04810"
     CDS             <1..1041
                     /locus_tag="VFZ80_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011575372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX6286784.1"
                     /translation="YVYRGELRIEISEVDQSDVSETKTKIMLNLGNPDLAFGTSSLPA
                     DGVGLARMEFIISESIRAHPMALLDPDAVEDADERAGIETMIARYESGADYFVERLSE
                     GVGTIAAAFYPRPVIVRMSDFKTNEYASLVGGRHFEPKEANPMLGFRGASRYAHPAYR
                     DGFALECAAMKRARDAMGLTNIKLMIPFCRRVEEGERVLEVMAENGLRRGENGLEVYV
                     MCEIPNNVVQVDAFSRVFDGFSIGSNDLTQLVLGVDRDSELVAFDFDERDEGVIEMLR
                     QVITGAHRNGKHVGICGQGPSDFPDLAQRLVEFGIDSISLNPDTIVQTRRVVADVESM
                     LEKSRAVMPVGG"
     gene            1242..1688
                     /locus_tag="VFZ80_04815"
     CDS             1242..1688
                     /locus_tag="VFZ80_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6286785.1"
                     /translation="MKKILLATDGSEHSIRAAETAGLLSSALDVMVDVINVVSDATLV
                     TTGPIHEYARVEQVAMTQRELHEALGADFVNEAANVVRKTGGQVGTTKVLIGSPAREI
                     VGYADENEVDCVVMGRRGLGDAAGLLMGSVSHKVGHLTERTLITTE"
     gene            complement(1729..2385)
                     /locus_tag="VFZ80_04820"
     CDS             complement(1729..2385)
                     /locus_tag="VFZ80_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6286786.1"
                     /translation="MTVSNGLAIVAATWLVIGFSIAFVMRRRGHDFWVWAALGCVLGP
                     LAVPLAIERGRYHSIEYQSPDVRDSHGSFDVLVGIDGSAESIAAAKEALLLFGGWITS
                     MTLAKVLDYDSGGSYTGAESRAAAIEELRSVASELRFEPTLTRLLFGRADEALMEYAR
                     GAGVDLIVVGACGHGLSERLFGSVTSRLVGGCDVPVFVGPRRRDADMSPGEKVRRGAI
                     "
     gene            complement(2528..2830)
                     /locus_tag="VFZ80_04825"
     CDS             complement(2528..2830)
                     /locus_tag="VFZ80_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286787.1"
                     /translation="MPTRRSHFTEMVLGIAGVLAAGVGAWMYYVPADWFLGGLAEGWY
                     FGMFIAAGLLLATAFGLFARMTYVDDRAYTTPVVVSTILALAALGGAIAFAVILII"
     gene            complement(3050..3412)
                     /locus_tag="VFZ80_04830"
     CDS             complement(3050..3412)
                     /locus_tag="VFZ80_04830"
                     /inference="COORDINATES: protein motif:HMM:NF036463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5335 family protein"
                     /protein_id="HEX6286788.1"
                     /translation="MSTTTELSLGSIDELATRLPSPGTADVTIEIVGEDIGDQIEVQR
                     LPWHALVYDRAAGILELSVGSRGRSVPVVFRHEIHGPKRLWIEEDGGLIRAISIEQGD
                     GIQTIVRFHARQAIESGD"
     gene            complement(3498..>3897)
                     /locus_tag="VFZ80_04835"
     CDS             complement(3498..>3897)
                     /locus_tag="VFZ80_04835"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HEX6286789.1"
                     /translation="RFLLEGAPLDVLGGEGLIIDQPQTRASWADLTPPILVEEPMWGS
                     TRGSDLTFAGIADLESGTVSFVVVDGDGLIIHEGEISGTPGEAVDFSEPVNLDEIPHP
                     GAGSIIAWEWAPDGSQRHVVEYPLTLLAKG"
BASE COUNT          785 a         1234 c         1176 g          702 t
ORIGIN      
        1 tacgtctatc gcggggagct tcgcatcgag atcagcgagg tagaccaatc agacgtgtcc
       61 gagaccaaga ccaagatcat gctcaacctc gggaacccgg atctcgcttt cgggacgtcg
      121 tcactacccg ccgacggtgt cggtctggcc cgcatggaat tcatcatctc ggaatcgatc
      181 cgcgctcacc ccatggccct tctcgacccc gacgcggtgg aggacgccga tgagcgcgcc
      241 ggcatcgaga cgatgatcgc ccggtacgaa agtggagccg actacttcgt ggaacggctc
      301 tcggagggag tcggcaccat cgcggctgcg ttctacccga gaccggtcat cgttcgcatg
      361 tcggatttca agacgaacga gtacgccagc ctggtcgggg gccgtcactt cgagccgaaa
      421 gaggccaacc cgatgctcgg tttcagggga gcgtcccgct acgcccatcc cgcatatcgt
      481 gatggcttcg ccctggagtg tgccgccatg aagcgtgccc gggacgcgat gggcctcacc
      541 aacatcaagc tgatgatccc cttctgtcgt cgtgtcgagg aaggggaacg agttctcgaa
      601 gtcatggcag agaatgggct tcggcgcggt gagaacgggc tcgaggtcta cgtcatgtgc
      661 gagatcccga acaacgtggt gcaggtcgat gcgttctcga gggtcttcga tggtttctcg
      721 atcgggtcga atgacctgac ccagctagtc ctcggagtcg atagggactc cgagctcgtt
      781 gccttcgact tcgacgagcg agacgagggc gtcatcgaga tgctgcggca ggtgatcacc
      841 ggagcacacc gaaacggcaa gcacgtcggt atctgtggtc aagggccatc ggattttccc
      901 gatctcgccc agcgattggt cgagtttggt atcgattcga tcagcctgaa ccccgacacg
      961 atcgtccaga cgagacgggt cgtggccgat gtcgagagca tgctcgagaa gtcgcgggcg
     1021 gtcatgcccg tgggtgggtg aagcccacag agatggtccg ttcgccggcg ctcccctgcc
     1081 cttttaaagg tgggtgccgt acagcgggct cctgagtccc agggtcggtg aacctcccgg
     1141 gttcccgccc gccgcctgcg ggcactgtcc ctgctgactc ctggctttcg gccctagctg
     1201 ctagccggtc tgagaggcac gatctcgacg gaaggagcga aatgaagaag atcttgcttg
     1261 caaccgacgg gtcggaacac tcgattcgag cagcagagac cgccggcctg ctctcctctg
     1321 cgctcgacgt catggtcgac gtgatcaacg tcgtttctga cgcgacactg gtgaccaccg
     1381 ggccgatcca cgagtacgcc cgagtcgagc aggtcgcgat gacacaacgt gagctgcacg
     1441 aagcccttgg agcggacttc gtcaacgagg cagccaatgt cgtccggaag accggtggcc
     1501 aggtgggaac caccaaagtc ctcatcggat ctcccgcgag ggaaatcgtt ggttatgccg
     1561 acgagaacga agtcgactgc gtcgtcatgg gtcggagggg gctcggagac gcggcggggt
     1621 tgctgatggg gagcgtgtcg cacaaggtcg gccatctcac cgaacgaacg ttgatcacaa
     1681 ccgagtagac cacagcccac cgtcccggga gccggcaatt gaccagcttc agatcgctcc
     1741 ccgacgaacc ttctcgcccg gactcatgtc tgcgtctctt ctcctcgggc caacgaacac
     1801 cgggacatca cagccgccga ccaggcggct ggtcacactg ccgaacagtc tttcggagag
     1861 gccgtgccca caggccccta cgacgatcag atcgacaccc gccccacggg cgtactccat
     1921 gagtgcctcg tcggctcttc cgaacaacag ccttgtcagc gtcggctcga aacgcagctc
     1981 ggaggcaaca cttcgtagct cctcgatagc cgccgcccgg gattcggctc cggtgtatga
     2041 cccaccggag tcgtagtcca gcactttcgc cagggtcata ctggttatcc acccgccgaa
     2101 caggagaaga gcctccttcg ctgcagcgat ggactctgct gagccgtcga tccccacaag
     2161 cacgtcaaag gagccatgcg aatcccgcac gtctggactc tggtattcga ttgagtggta
     2221 cctcccccgc tcgatggcaa ggggcacggc caaggggccc agcacacagc caagtgccgc
     2281 ccacacccag aagtcgtggc cccttctacg catcacgaag gcgatcgaga acccaatcac
     2341 gagccaagtc gcagcgacga tggccaggcc gttagagact gtcatctgag ctcctccttt
     2401 ccatgacctc gagtagatcg acgagctgtg ggaaagggaa gggtcgttgg tcccatctcc
     2461 caagacgtga gaacgagacg cgaaaccggg actctctgat cgagagtccc ggtttcgatc
     2521 tgtgcgtcta gatgatcagg atcacggcga atgcaatggc gccgcccaga gcggccaacg
     2581 ccaggatcgt gctgacgacc acaggcgtcg tgtacgcccg atcatcgacg taggtcatcc
     2641 gagcgaagag cccgaatgcg gttgcgagca gcagacccgc cgcgatgaac atgccgaagt
     2701 accagccttc agcaagccca ccgaggaacc aatcggccgg cacgtagtac atccacgccc
     2761 cgactcctgc cgccagcaca ccggcgatcc ccagcaccat ctcggtgaaa tgggaccgcc
     2821 gtgtcggcat ccgggtgatc tccgtagttg gcctttcgcg aagagctgtc acagagatca
     2881 cctcctttcc tggaagaccc gttgcgggtc atccatcgcc atcgtccgcg atcgaggtga
     2941 gggcgaccag aggctttcgg cccgtcttca ggtgacgttc tccgataacg gacgaaacga
     3001 tcgctgtgcc ggccgtgaat tcagtcagtt gatcgttcgg caacggtcgt caatcccccg
     3061 actcgatggc ctggcgtgcg tggaagcgaa cgatggtctg aatgccatcg ccctgctcga
     3121 tggaaatcgc tctgatcaga ccaccgtctt cctcgatcca cagccgcttc ggaccgtgga
     3181 tctcatgacg gaacaccact ggaacgctcc tgcctcgcga tccgacggat agctcgagga
     3241 tccccgccgc cctgtcgtag acgagggcgt gccacggaag tctctgcacc tcgatctgat
     3301 caccaatgtc ctcgccaacg atctcgatgg tcacatcagc cgttcccggt gaagggaggc
     3361 gagttgcgag ctcatcgatc gaaccaagtg acagttctgt cgtagtggac atgaggcctc
     3421 cttgcttgcc tgccacgagc gtgcaccgct ttgtgtgtca gggccagagg aaaaggtccc
     3481 gagagttcga gctggggtca gcccttagcc agcaatgtca gcggatactc gacgacatgg
     3541 cgctgcgacc cgtcaggcgc ccattcccaa gcgatgatcg agccagcccc gggatgggga
     3601 atctcatcga gattgacagg ttctgagaaa tcaacggcct cgcccggggt gccggagatc
     3661 tcaccctcgt gaatgatgag gccgtcgcca tccaccacga cgaacgagac cgtccccgat
     3721 tccaggtcgg cgattccggc gaaggtcagg tcgcttccac gagtcgagcc ccacatcggc
     3781 tcctcgacga gtatcggtgg cgtcagatcc gcccacgagg ctcgagtctg cggctggtcg
     3841 atgatgagac cctccccacc gaggacatcg aggggcgctc cctccaggag gaaacgc
//
LOCUS       DASUTA010000241         3409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1625341, whole genome shotgun sequence.
ACCESSION   DASUTA010000241 DASUTA010000000
VERSION     DASUTA010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625341"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3065
                     /gene="carB"
                     /locus_tag="VFZ80_04840"
     CDS             <1..3065
                     /gene="carB"
                     /locus_tag="VFZ80_04840"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX6286790.1"
                     /translation="ADATYIEPITPEVVTEIIARERPDALLPTLGGQTALNVASQLAR
                     SGVLDKHGVEMIGAGLEAIDRAEDRGLFKETMEGIGLEVPQAGYARSMSEAVKIAREV
                     GYPVMVRPSFILGGGGTGIADDEEEFLEVARKGLDTSPVHEILVEESVLGWKEFELEV
                     MRDGNGNGVIVCSIENFDPMGVHTGDSITVAPIQTLSDREYQDMRDDAIRVLEAIGVE
                     TGGSNVQFAVDPDTGRRLVIEMNPRVSRSSALASKATGFPIAKIAALLAVGFTLDEIA
                     NDITKATPASFEPALDYTVVKIPRFDFAKFPSASTRLGTSMRSVGEAMAIGRTFPEAL
                     QKALRSLENGRAGLNADPGERALFRDVSDTDLESMVATPTPDRVFAIGEALRRGWVVD
                     RVATLSKVDPWFIDQMAEVIEVRLELEESRGEDLLREAKRMGFSDRQIAHLWGVSEGD
                     VRSLRKEKGLTRTYKTVDTCAAEFEAHTPYMYGTFEDESEVQPADNPRVVVLGSGPNR
                     IGQGIEFDYCCVHASFALKEAGFETVMVNCNPETVSTDYDTSDRLYFEPLTTEDVLEV
                     IEAEQPEAVIVQLGGQTPLNLAEHLEKAGVRIAGTSPASIAMAEDREQFAEICRNLGL
                     SQPASGTAMSAADAQEVVDQIGLPVMVRPSFVLGGRKMRVVYSLEELGQYVRDIYGEG
                     DPEVTANPILIDRFLESAVEVDVDAVFDGRELLVGGIMEHVEEAGVHSGDSGCVTPPP
                     TISDEALKEILRGTNELARALSVEGLINIQFAVKGDEVYVLEANPRGSRTVPFISKAK
                     SLPLAAIAARVMMGATLAELRDEGLVPESVSEPGFVAVKDAVLPWDRFPEEDTILGPE
                     MRATGEVMGIGPDVGVAYGKALLAAGNTLADRGTVFLSFADRDKPLGLAVAQAFTMLG
                     FHLIATGGTARYLEHHAIAAELVNKIGEGPRDIGERLENSEVQLVVNTPRGGRARSDG
                     LSIRQIAKRNFIPCVTTVQGGLAVARSLRAGPGAIHRPRSLQEWHRKT"
     gene            3047..>3409
                     /locus_tag="VFZ80_04845"
     CDS             3047..>3409
                     /locus_tag="VFZ80_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX6286791.1"
                     /translation="MASQDLSTSLGALELRSPLIAASGTVGSVWEWAETCDVSLYGAA
                     VAKSVSPEPWEGRPPPRLAPTSTGMLNGIGIQNPGIGRWVDEMQPRVGSLGVPVWGSA
                     VAHDASGFGVVAKELEAAG"
BASE COUNT          635 a         1039 c         1154 g          581 t
ORIGIN      
        1 tcgccgacgc cacatacatc gagccgatca cgcctgaagt cgtaaccgag atcatcgccc
       61 gtgagcgtcc cgacgcccta ttgccgacac tggggggtca aacggctctc aacgtcgcct
      121 ctcagttggc caggtccgga gtgctcgaca agcacggtgt cgagatgatc ggggcgggtc
      181 tcgaggcgat cgatcgggca gaggatcggg gtctattcaa ggagacgatg gagggcatcg
      241 ggctcgaggt cccccaggcg ggttacgccc gatcgatgag tgaggctgtg aagatcgcca
      301 gggaagtcgg ctatccggtg atggtgcggc cttcgttcat ccttggagga ggcggcaccg
      361 gcatcgcaga tgacgaagaa gagttcctcg aggtcgcccg gaagggcctg gacacctcgc
      421 cggtccatga gatcctcgtc gaggagtctg ttctggggtg gaaggaattc gagctggagg
      481 tgatgcgaga cggcaacggc aacggcgtca tcgtctgctc catcgagaac ttcgacccca
      541 tgggcgttca caccggcgac tcgataacag tcgctcccat ccagaccctg agtgaccgcg
      601 agtaccagga catgcgggat gatgccatca gggtcctcga ggcgatcggc gtcgagaccg
      661 gcgggtccaa cgtccagttc gcggtcgatc ccgacaccgg caggagactg gtcatcgaga
      721 tgaacccgcg cgtcagccgc tcctcggcgc tggcatcgaa ggcgaccggg ttcccgatcg
      781 caaagatcgc tgctctgctc gccgttggct tcacgttgga cgagatcgcc aacgacatca
      841 ccaaggccac cccggcatcg ttcgagcccg ccctggacta caccgtggtc aagatccccc
      901 gcttcgactt cgccaagttc ccgtcggctt caacgaggtt ggggacctcg atgcggagtg
      961 tgggagaggc gatggccatc gggcgcacct tccccgaggc cctgcagaag gcgctgcgca
     1021 gcctggagaa cgggcgagcc ggtctcaacg ccgaccccgg agagagggcg ctcttccggg
     1081 atgtgagcga caccgatctg gagtcgatgg tggcaacacc gactcccgac cgggtgttcg
     1141 ccatcggaga ggccctgcgc cgaggctggg tcgtcgaccg ggtggcaaca ctgtccaagg
     1201 tcgacccgtg gttcatcgac cagatggcgg aagtcatcga agttcggctc gagctcgagg
     1261 agagccgtgg cgaggacctt cttcgcgagg cgaagcgaat gggcttctcc gaccgccaga
     1321 tcgcacatct gtggggagtg tcggaaggag acgtacgcag cttgcgaaag gagaaggggc
     1381 tcacccggac ctacaagacg gtggacacat gtgcagcgga gttcgaggct cacaccccct
     1441 acatgtacgg caccttcgag gatgagagcg aggtgcagcc ggccgacaat ccacgagtgg
     1501 tcgtcctcgg atcgggaccg aatcggatcg ggcagggcat cgagttcgac tactgctgtg
     1561 tacacgccag cttcgctctc aaggaggctg gcttcgagac cgtcatggtc aactgcaacc
     1621 ccgagacggt gtcgaccgac tatgacacct cggaccgtct ctacttcgaa ccgctgacca
     1681 ccgaggacgt tctcgaggtc atcgaggcag agcagcccga ggcggtgatc gtgcaactgg
     1741 gtggccagac ccctctcaat ctcgccgagc atctggagaa agccggggtc agaatcgcgg
     1801 gtacgtcgcc cgcttcgatc gccatggccg aagatcggga gcagttcgcc gagatctgcc
     1861 ggaatctcgg gttgagccag ccggcgtcgg gaactgcgat gtccgccgca gatgcccagg
     1921 aggtcgtcga ccagatcggc ctgccggtca tggtccggcc gtcgttcgtg ctcggtggac
     1981 gaaagatgcg cgtggtctac agcctcgagg agctcggcca atacgttcgg gatatatacg
     2041 gagaaggcga ccccgaggtc acagccaatc cgatcctcat cgatcgcttc ctcgaatctg
     2101 cggtcgaggt cgatgtcgac gcggtgttcg acggccggga gctcttggtc ggcgggatca
     2161 tggagcatgt cgaagaggca ggtgtccact ccggggactc cggctgtgtg actccaccac
     2221 ccactatctc agatgaggcg ctgaaggaga tactcagggg aacgaatgag ctagctcgcg
     2281 cattgagcgt cgagggtctc atcaacattc agttcgcggt caagggcgac gaggtctacg
     2341 tgctcgaggc caacccgaga gggagccgca ccgtgccctt catctcgaag gcaaagtcgc
     2401 tccccttggc ggcgatcgcc gccagggtga tgatgggcgc cactctcgcc gagcttcgag
     2461 atgaaggctt ggtgcctgaa tccgtctccg agcccgggtt cgtagccgtg aaggacgccg
     2521 tccttccgtg ggaccgcttc cctgaggagg acaccatcct cggcccggag atgcgggcca
     2581 ccggcgaggt catgggcata ggccccgacg tgggggtcgc ctacggaaag gcgctcctcg
     2641 ccgcaggcaa cacccttgcc gacaggggaa cggtctttct ctccttcgcc gaccgggaca
     2701 agccactggg tctggcagtt gcccaggcct tcaccatgct cgggttccat ctcatcgcca
     2761 ccggcggcac ggcgcgctac ctcgaacacc acgccatagc ggcggagctg gtgaacaaga
     2821 tcggtgaagg cccaagagac atcggagagc gcctggagaa ctccgaggtc cagttggtgg
     2881 tgaacactcc ccggggaggc cgggcccggt ctgacggcct gagcatccga cagatcgcca
     2941 agcgcaactt catcccttgc gtcaccacag ttcagggggg tctcgccgtg gcgaggagtc
     3001 tgcgcgccgg tcctggcgca atccacaggc cgaggagcct ccaggaatgg catcgcaaga
     3061 cctgagcacc agtcttggtg ccttggagct gcgctcgcct ctgatcgcgg cgtcggggac
     3121 ggtgggatcg gtctgggagt gggcggagac gtgtgatgtc tcgctctacg gggctgccgt
     3181 cgccaagtcg gtctcacccg agccgtggga gggacggccg ccgccgcggt tggcccccac
     3241 ctcgaccggg atgctgaatg ggatcgggat ccagaatccc gggatcggca gatgggtcga
     3301 tgagatgcag cctcgagtcg ggtcgctagg tgtccccgtg tggggatctg cggtggccca
     3361 cgacgcctcc ggattcggtg tcgtggccaa ggagttggag gcggctgga
//
LOCUS       DASUTA010000242         2899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1755128, whole genome shotgun sequence.
ACCESSION   DASUTA010000242 DASUTA010000000
VERSION     DASUTA010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755128"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="VFZ80_04850"
     CDS             <1..1134
                     /locus_tag="VFZ80_04850"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6286792.1"
                     /translation="YAESFINTRAMTEMGWDVTRAPGGRVTSPSDMAALRERLAVSDV
                     EPAMAGTEALLRELNRYGLTGAIDASGFGITADSYDAFLRLFAEGERGFRARLLVGAA
                     QPGREMEDLERWTSSLSLEGGHELIRYLGAGEVLDYAAHDLEGLAPKDIRDRLDGLRS
                     VSSFLADRGWPAHVHAILDSSITTLLDAWEAVDGTTLSDLRFAICHADQIGEENLRRV
                     RDLGVGITIQNGMSMRGRDCLPTWGADLVASSPPIRSMLDLGIPVAAGTDGTVACAYN
                     PWRCIAWMVTGESVDGSPPRSEEQRLTLDEALRLYTSAGAWFSFEEDSRGNLAQGSHA
                     DLAVLSDDPLRISPGSLSTIESVLTVVGGVIAHSTLESAEVGE"
     gene            1131..1529
                     /locus_tag="VFZ80_04855"
     CDS             1131..1529
                     /locus_tag="VFZ80_04855"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019075034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6286793.1"
                     /translation="MRDAINPPSLPPPPGYSQVVRISRGTTIYIAGQVAWDEEGNLVG
                     AGDFEAQTRQVFANLVAALHSASADVADLVKIGIYVVDHDPEKLDVIRRVRNALFGDI
                     TPPASTLLGVSVLAVPGLMIEVDGVAFVED"
     gene            complement(1569..2273)
                     /locus_tag="VFZ80_04860"
     CDS             complement(1569..2273)
                     /locus_tag="VFZ80_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286794.1"
                     /translation="MLEVTDLTAGYGKAQALYGISLLVDEGEIVTILGPNGAGKTTLV
                     NSIAGVIKPTGGAVVLDGTDMLTLAPHQISSHGVALVPEGRRIFPRMTVSDNLDIGAY
                     GKSARDGREENLSRVHEVFPRLAERANQEAGTLSGGEQQMLAIGRALMSRPKLLLLDE
                     PSLGLAPIIVSGIFEVLREINSSGVSILLVEQNAVEALDLASRGYVLEEGHIVGEDES
                     EALLNDERLRKAYLGL"
     gene            complement(2266..>2899)
                     /locus_tag="VFZ80_04865"
     CDS             complement(2266..>2899)
                     /locus_tag="VFZ80_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286795.1"
                     /translation="LIGPNGSGKSTLINILSGFYPCDQGSINFDGEEATELPAHRIAA
                     LGVARTYQIPRPFDTMTPRENIAVSYMFGHSRHSLSDARDAAQQWLEFTGLGDVADIS
                     IGELTLHQLKFLELARALATQPTLLLLDEVLAGLNPSEMNESLEMIRRIHEKGVTILI
                     VEHVVRAVMALSDRLVVLDQGRVISEGDPEAVMTDPAVVAAYLGEKRPGA"
BASE COUNT          513 a          945 c          897 g          544 t
ORIGIN      
        1 tatgccgagt cgttcatcaa cacccgcgcc atgaccgaaa tgggttggga cgtgacccga
       61 gctccgggtg gacgcgtgac atcgccatcc gacatggcag ctctcaggga gcgattggca
      121 gtgagcgacg tcgagccggc gatggccgga accgaggcgc tcctgcggga gctcaaccgg
      181 tacgggctca ccggggccat cgacgcctcc ggattcggga tcaccgccga ctcctatgac
      241 gcattccttc gcctcttcgc cgagggcgag cgcggattcc gtgcgagact cctcgtcggg
      301 gcggcacagc ccggacgaga gatggaggat ctcgagcgat ggacttccag cctctccctc
      361 gaaggcggcc acgagctgat ccgatacctt ggggctgggg aagtgctgga ctacgcggcc
      421 cacgacctcg aaggcctcgc tcccaaggac atccgcgatc ggcttgatgg cctgagatcg
      481 gtttcttcgt tcctcgccga ccggggctgg ccggcgcacg ttcacgcgat cctcgactcc
      541 tcgatcacga ccttgctcga tgcctgggaa gcagtggacg ggaccaccct ctccgacctt
      601 cgattcgcca tctgccatgc cgatcagatc ggtgaggaga acctgcgacg cgttcgtgac
      661 ctcggcgtgg gcatcacgat ccagaacggg atgtcgatgc gtggacgcga ctgtcttccc
      721 acctgggggg ctgacctcgt ggcctcctcc ccccccatcc gctccatgct cgatctcggg
      781 atcccggtcg ccgccggaac cgacggaacc gtggcgtgcg cgtacaaccc ctggcgttgc
      841 atcgcctgga tggtcaccgg cgagtcggtc gacggctctc caccacgcag tgaggagcag
      901 aggctgactc ttgacgaagc gctccggctc tacacgtcgg ccggggcgtg gttctccttc
      961 gaagaagact cgagagggaa cctggctcag ggaagccacg ccgatctggc ggtgctcagc
     1021 gatgaccctc tccgtatctc cccggggtca ctctcgacca tcgagtccgt gctcaccgtc
     1081 gtcggcggtg tgatcgctca ttccactctc gagtctgctg aagttggcga atgagagacg
     1141 ccatcaaccc gccttcgctt cccccacctc cgggatattc acaggtggtc aggatctcga
     1201 gaggcacgac catctacatc gccggacagg tcgcctggga cgaagagggc aacctcgtcg
     1261 gcgccggtga cttcgaggcc cagacccgcc aggtcttcgc caacttggtg gcagctctcc
     1321 actcggcttc cgccgacgta gccgatctgg tgaagatcgg gatctacgtc gtcgaccacg
     1381 accccgagaa gttggacgtg atacgtcggg tgcgcaacgc actgttcggt gatatcacgc
     1441 caccagccag cacactgttg ggggtgagtg ttctcgccgt gccagggctg atgatcgagg
     1501 tcgacggcgt cgccttcgtg gaggactgag cttcccagct acctctctct gctctcggcc
     1561 taacccctct acaacccgag ataggcctta cgcagcctct cgtcgttgag gagagcttcc
     1621 gactcgtcct cacccacgat gtggccctcc tcgaggacgt agccgcgaga ggcgagatcg
     1681 agggcctcca ccgcgttctg ctcgaccagg agaatagaga cacccgagct gttgatctcc
     1741 ctgaggacct cgaagatccc ggacacgatg atcggtgcca gacccagact gggctcgtcg
     1801 aggagtagca gctttggcct cgacatgaga gcccgcccga tcgccagcat ctgttgctca
     1861 ccgccactca gagtgcccgc ctcctggttg gcacgctcgg cgaggcgggg aaacacctcg
     1921 tggaccctgc tgaggttctc ctcgcgtccg tcccgggcgc tctttccgta ggcaccgatg
     1981 tcgagattgt cactgacggt catccgggga aagatccgcc gcccctcggg taccagggca
     2041 acaccatgac tgcttatctg gtgcggcgcc aatgtgagca tgtcggtccc atcgagcaca
     2101 actgctccac cggttggctt gatgactccg gcgatcgaat tgaccagggt cgtcttcccg
     2161 gccccgttgg ggcccaggat ggtgacgatc tcaccttcgt cgacaagcag ggaaatgccg
     2221 tataaggcct gtgccttgcc atagccggct gttagatcgg tgacttcaag cacccggccg
     2281 cttctcccct agataagccg ccaccacggc gggatcggtc atcacggcct cggggtctcc
     2341 ctcactgata acacgaccct ggtcgagcac gacgaggcgg tcggacaatg ccatgaccgc
     2401 ccgaacgacg tgctccacga tcagaatggt gacgcccttt tcatggatcc gcctgatcat
     2461 ctccagcgac tcgttcatct cactcgggtt gagaccggcg aggacctcgt cgagcagcag
     2521 cagagtgggc tgagttgcca gcgcccgggc cagctcgaga aacttcagct gatgcaaggt
     2581 gagctcgcca atgctgatgt cggcgacatc gcccaggccc gtgaactcga gccattgttg
     2641 tgcagcgtcg cgggcgtcgg acagggagtg acggctatgc ccgaacatgt agctgacggc
     2701 gatgttctcc cgtggcgtca tggtgtcgaa cggcctcggg atctgatagg tacgggcaac
     2761 gccgagggcc gcaatccggt gcgcagggag ctcagtggct tcctcgccgt cgaagttgat
     2821 cgagccctgg tcacaggggt agaagccact gaggatgttg atcagtgtcg atttgcccga
     2881 gccgttgggc ccgatcagg
//
LOCUS       DASUTA010000243         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_458903, whole genome shotgun sequence.
ACCESSION   DASUTA010000243 DASUTA010000000
VERSION     DASUTA010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458903"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VFZ80_04870"
     CDS             complement(<1..1178)
                     /locus_tag="VFZ80_04870"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286796.1"
                     /translation="MPTPSEPRRSLFDDFIDLRDSLRDKRIGGGGREVCAALTGALDK
                     GIASLAGSLPAGVAVLALGGYGRRELSPFSDVDLMLLHDMDDPAQPAADLFRPLWDAK
                     LRVGHSVRTVRESAAAARERFDTQTTLLTSRLVVGDREHFETLMEAVIAVTRARPLRR
                     HLVAEERRRRRETPFLGMAADVKDGRGGLRTLQGFEWERRRESLIGRFSAEPGPEEDS
                     ARESLLRIRNAIHMATGRRHDEFSVELREQVARWLESDVFEVSEQLVTAMQTVDGLAV
                     SRWPEVLDEEETVPRRVWHRVSRRPEPVALDRPPTLAELLWLLETGERGSIAFERLRA
                     AGQLDDILPEWEVVRGLPQLAPFHEHPVDVHLWRTVAEMRSLVARPGHYQRVAEELDD
                     "
     gene            complement(1162..1500)
                     /locus_tag="VFZ80_04875"
     CDS             complement(1162..1500)
                     /locus_tag="VFZ80_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX6286797.1"
                     /translation="MKLIIAFIKPFKLEQVGEALKSVGVSGMSVSDISGFGRQAGQRE
                     VYRGAEYEVDFVPKVRLEVLVTDDIAGVAVDAIEKSAHTGEIGDGKIIVVSVYDAVRI
                     RTGERGADAV"
     gene            complement(1497..>2301)
                     /locus_tag="VFZ80_04880"
     CDS             complement(1497..>2301)
                     /locus_tag="VFZ80_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX6286798.1"
                     /translation="GAIILGPRIGKYGRDGRPRAIPGHNIAFVVLGVLILFIGWFGFN
                     PGSELLADEFVPQIALKTLLAGAAGAVVAMTVNWTRDGKPDVSIASNGLLAGLVAITA
                     PVGAVETWAAIVIGAVGGLIVVFSVSMFDRLKIDDPVGAISVHGVVGTWGTLSIALFA
                     VYDDAFLGRENAGLLYGGGLDQLWTQLIFVVVHFVWVTAAAGLLFLLIKKTIGLRVSP
                     EEELAGLDVEEHGSPGYGHDPAHVGSGVVTGALSGADRQSIPNSEGGSE"
BASE COUNT          429 a          794 c          704 g          374 t
ORIGIN      
        1 tagtcgtcca gctcctccgc cacacgctgg tagtgacccg gcctcgccac caggctccgc
       61 atctcggcaa ccgtcctcca caggtggaca tcgaccggat gctcgtggaa cggagcgagt
      121 tggggaagac cgcgtacgac ctcccattcg ggcaggatgt cgtccagttg cccggccgct
      181 cgaagacgct cgaaggcgat gctcccccgc tcccccgtct ccagcagcca gaggagctcg
      241 gccagggtcg ggggccgatc gagcgcgaca ggctcggggc ggcgtgaaac gcggtgccag
      301 acccgccgtg gcactgtctc ttcttcgtcg agaacctcgg gccatcggct gacggcgagc
      361 ccatcgaccg tctgcatcgc cgtcaccaac tgctcggaga cctcgaacac atccgactcc
      421 agccagcgag ccacctgctc tcgcagctcg accgagaact cgtcgtgacg tctccccgtg
      481 gccatgtgga tcgcgtttcg gatgcgaaga agtgattcgc gggctgagtc ttcctcgggg
      541 ccgggctcgg ccgagaagcg cccgatcaga gactcccggc gtcgctccca ctcgaatccc
      601 tgcaacgtcc tgagaccccc gcgaccatcc ttgacgtcgg cggccatccc caggaacggg
      661 gtttccctac gcctccggcg ctcttcggcc accagatgtc gtctcaaagg ccgggcgcga
      721 gtgacggcta tgaccgcctc catcagtgtc tcgaagtgtt ctcggtcacc gacgacgaga
      781 cggctcgtga ggagggtggt ctgggtgtcg aacctctctc tcgccgccgc cgccgactcc
      841 ctcacggttc gaaccgagtg accgacgcgg agcttggcgt cccagagcgg tcggaacaga
      901 tcggccgccg gctgcgcagg atcgtccatg tcgtgcagca gcatcaggtc gacgtcggag
      961 aaggggctca gttcgcgacg cccataaccg cccagggcca ggaccgcgac acccgctggg
     1021 agggagccag ccagagacgc tattcccttg tcgagggccc cggtcagcgc agcgcacacc
     1081 tctcgacctc caccgccgat gcgcttgtca cgcagcgaat cgcggagatc gatgaagtca
     1141 tcgaacagag atctccgtgg ttcagacggc gtcggcaccc cgctccccgg tgcggatacg
     1201 cactgcgtca tagacagata cgacgatgat cttcccgtcg ccgatctcac cggtgtgcgc
     1261 cgacttctcg atggcgtcga ccgccactcc ggcaatgtcg tcggtgacca ggacctcgag
     1321 acgcaccttg ggcacgaaat cgacttcgta ctcggcaccg cgatagacct cgcgctggcc
     1381 tgcctgtctc ccgaagccgg agatgtcgct cactgacatg cccgagactc cgacagactt
     1441 cagcgcctcg ccgacctgct ccaacttgaa cggcttgatg aaggcgatga tgagcttcat
     1501 tcgctccctc cttccgagtt cggaatcgac tgcctgtcgg ctccgctgag cgcgccggtg
     1561 acaacgcccg agccgacatg agcgggatcg tggccgtagc caggtgaccc gtgctcctcg
     1621 acgtcgagac cggcgagctc ctcttcgggg ctgacccgca gaccgatggt cttcttgatg
     1681 aggagaaaca acaatccagc agcagccgtg acccagacga agtgcacgac gacgaagatc
     1741 agctgggtcc acagctggtc caggccgcct ccgtagagca ggccggcatt ctcccgaccg
     1801 aggaatgcgt cgtcgtagac cgcaaacaat gcgatcgaga gcgtgcccca ggtaccgacg
     1861 acaccgtgga ccgagatggc gcccacaggg tcgtcgatct tgagtcggtc gaacatcgac
     1921 acagagaaga ccacgatgag cccacccacg gccccaatca cgatcgcggc ccaggtctcg
     1981 acggcgccga ccggagcagt gatggcaacg agcccggcca agagaccgtt cgacgctatc
     2041 gagacgtccg gcttcccgtc tctggtccag ttgacggtca tcgccactac cgcacccgcc
     2101 gcgccggcga gaagggtctt gagcgcgatc tggggcacga actcatcggc gagcagttcg
     2161 gatccggggt tgaagccgaa ccatccgatg aacaggatca agactccaag gacgacgaag
     2221 gcgatgttgt gccctggtat ggcacggggc ctgccgtccc tgccgtactt gccgatgcga
     2281 ggtccgagga tgatcgcacc c
//
LOCUS       DASUTA010000244         2386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1496458, whole genome shotgun sequence.
ACCESSION   DASUTA010000244 DASUTA010000000
VERSION     DASUTA010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496458"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="VFZ80_04885"
     CDS             complement(<1..120)
                     /locus_tag="VFZ80_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX6286799.1"
                     /translation="MGKSPSEEIDDMIEDLGDWRAEKLSQLRRLIHEADPEVVE"
     gene            complement(204..794)
                     /locus_tag="VFZ80_04890"
     CDS             complement(204..794)
                     /locus_tag="VFZ80_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286800.1"
                     /translation="MSEVVGLFPSRAQAERAIQELDDRGYEATNLGYVDRYRDESGEV
                     VTDEGYFDDDDHTDEDHEVAHEAGKGAAGGAVGGAAVGAGTALLASAGLVLIPGIGPF
                     LAAGTLAGVLGSAAVGAAGGAALGGAAGAIFGAVDDHEHADHETSRHYRERVSAGETM
                     VSVNVDDDSDVEVTEVLSAAGASRVDIYGGQGWVAV"
     gene            complement(856..1041)
                     /locus_tag="VFZ80_04895"
     CDS             complement(856..1041)
                     /locus_tag="VFZ80_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286801.1"
                     /translation="MSDDRPIAATSFCVVPGCTDLATRERTIVQGPDVERQIAVCWKH
                     EEGELDIESLDLGDWGE"
     gene            complement(1078..2058)
                     /locus_tag="VFZ80_04900"
     CDS             complement(1078..2058)
                     /locus_tag="VFZ80_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6286802.1"
                     /translation="MAGRRTNLALLWCTVLAVVTGVGAFLVGTPNGAWIVVAHGVVAL
                     GVVILAPWKTMIASRGMERQRSGRAFSLLLTMAIVGVLVSGFALITGVRFVGPLTTMQ
                     VHVGLGLGAIVGTLVHLGQRPTPHRRADLDRRNLLRTGGLMAGAGALWLLIESTLDLA
                     HLPGGARRFTGSHEITDPNAIPATQWINDRVQHLERTSHVVRIAERTLKTSDLDGTDE
                     IEATLDCTGGWFTTQTWSGTRLDQLLDVTTGHSVVVRSTTGYWRRFPIEHASRLLLAT
                     RLAGELLPDGNGGPVRLVAPGRRGYWWVKWVEAVEVDDRPPWWQPPLPVA"
     gene            2113..>2386
                     /locus_tag="VFZ80_04905"
     CDS             2113..>2386
                     /locus_tag="VFZ80_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6286803.1"
                     /translation="MTERLDNWMDQYVLAWSSNDSEHIRALFTEEAVYDPQTADGEWD
                     GIEEIVARWQEIDDSEDNWDFEWRPVVETEEVAVVRGRTHYFEPEAI"
BASE COUNT          435 a          831 c          702 g          418 t
ORIGIN      
        1 ttcgaccact tccgggtcgg cttcatgaat caggcgccgc aattgcgaga gcttctcagc
       61 cctccagtca cccaggtcct cgatcatgtc gtcgatctcc tcagaagggc tcttgcccat
      121 ttccccccct tcgattccgc cctgtgggaa agcgaaacgg gtgccgggac ccggcacccg
      181 ttcgacagct ccaagctggg gcgtcagacc gcaacccagc cttgaccacc gtagatatcg
      241 acccggctcg cccctgccgc actcagcacc tcggttactt cgacgtccga atcgtcgtcg
      301 acgttgacag agaccatggt ctcgcccgcc gagacgcgct cccggtagtg ccgggatgtc
      361 tcgtggtcgg cgtgctcgtg atcgtcgaca gcaccaaaga tggctcccgc ggcgcccccg
      421 agggcggccc caccggccgc gccgacagcg gcgcttccca ggaccccggc gagggtgccc
      481 gcggccagga atggtccgat acccgggatg agcaccaggc cggccgaggc gagaagggct
      541 gtccccgccc ccacggccgc tccgccgacg gcgccgccgg ctgctccttt gcccgcttcg
      601 tgtgccacct catgatcttc gtccgtgtgg tcgtcgtcat cgaagtaacc ctcgtcggtg
      661 accacttcgc cgctctcatc ccgatagcgg tcgacgtatc caagattcgt ggcctcgtag
      721 ccacggtcgt cgagctcttg gatcgctcgc tcagcctgcg cacggctcgg gaagagccct
      781 actacctcac tcatgttcgc ctccaatgtc gattggcggc ggatcggtac ccatgagccg
      841 cagggtcgaa acggactatt cgccccagtc gcccaagtcg aggctttcga tgtcgagttc
      901 gccttcctcg tgcttccagc acaccgctat ctgacgctcg acgtcgggac cttgcacgat
      961 cgtccgctcc ctggtcgcca ggtcggtaca ccccgggacc acgcaaaacg aggtagccgc
     1021 gatcggtctg tcgtcactca tggccgcaac catacgcgcc gggattcggc tcccaagtca
     1081 cgccactggc aatggtggct gccaccatgg cggtcggtcg tccacttcga cggcctccac
     1141 ccacttcacc caccaatagc cgcggcgacc gggggccacc agcctcactg gccctccatt
     1201 tccgtcgggc aggagttcgc ctgcgaggcg cgtggccagc aacaggcggg aggcatgctc
     1261 gatcggaaac cttcgccaat acccggtcgt cgagcgaacc accacgctgt gacctgtcgt
     1321 cacatcgagg agctgatcga gcctcgtgcc agaccaggtc tgagtcgtga accaaccacc
     1381 ggtgcagtcg agagtcgcct cgatctcatc ggttccatcg agatcgctcg tcttcaatgt
     1441 ccgctcagcg attctgacga catgcgatgt gcgctccaga tgctgtactc ggtcgttgat
     1501 ccactgtgtg gcggggatcg cattcgggtc ggtgatctcg tgagagccgg tgaatcgacg
     1561 agcgccaccc ggtagatgtg ccagatcgag tgtgctctcg atcaggagcc agagtgcgcc
     1621 ggctcctgcc atcagccccc cagttcgcag cagatttcgt ctatcgagat cggcacggcg
     1681 atgcggcgtt ggtctctgtc cgaggtggac gagcgtcccg acgattgcgc ccagcccgag
     1741 cccaacgtgc acctgcatcg tggtcagcgg gcccacaaag cgcactcccg tgatcaaggc
     1801 gaacccggag accaggaccc cgacgatggc catagtcagc aacagcgaaa acgcccgccc
     1861 cgaccgctgt cgctccatac cccgtgaggc gatcatcgtt ttccatggcg ccagaatcac
     1921 gacaccgagc gcgactacgc cgtgtgccac aacgatccac gccccgttcg gggttccgac
     1981 caggaaggct cccaccccgg tgacgacggc gagaaccgtg caccacaaca acgccagatt
     2041 cgtgcgccgg ccggccatga caccgatgtt gcctgttgtc ttcagctagt gcagcgctaa
     2101 cttcgaacga cgatgaccga gagactcgac aactggatgg accaatacgt tcttgcctgg
     2161 agcagcaacg acagcgagca catcagagca ctcttcaccg aggaggccgt gtacgacccg
     2221 cagacggccg atggcgagtg ggacggaatc gaggagatcg tcgcccgctg gcaggagatc
     2281 gacgactccg aggacaactg ggacttcgaa tggcgcccgg tcgtcgagac cgaggaggtt
     2341 gccgtggtaa ggggccgcac ccactacttc gagccggaag ccatct
//
LOCUS       DASUTA010000245         3517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_459184, whole genome shotgun sequence.
ACCESSION   DASUTA010000245 DASUTA010000000
VERSION     DASUTA010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459184"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2002
                     /locus_tag="VFZ80_04910"
     CDS             <1..2002
                     /locus_tag="VFZ80_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286804.1"
                     /translation="AVGAMAQAIQDWRLVSRFPAGSVVFPDVSPDGSQVAVSSKNTPS
                     DVDLFAADGSLIGTLHGPNDPTVVAWQVDFSPDGRTIAVIYAGSDNIDEFRQLPDGFP
                     DLRTFDLTTMSTILELDTDHGWRYISHDLDGGRLALSFDREIRILDAVDGTEISRIEA
                     RSTVGRAQFLDDDTVLVPVGGSGFTSFDPNGGSEVDHLEVAGLIPAITAINDLRTHVA
                     YREGEQVRVTELSTGETIYDRTGESVQTLALDPDATRLAHSGFDPNITIDALDDADMG
                     LELTGILENVFSLTFMGESQLLSYGEDALLWNVSPEGVSELGGIPLQELQWGFQISPD
                     ERWLSYYVSTNSGIPPADPVDGLRWIDLSTGEETVISQGEINLVPGGFRSINPDFTMV
                     GALTQNGESTLRGLPAWDVIREFDACRGLLAITPDNTMGVLSGWSCDTPVPEAAHSVV
                     LDLESGDEIFPLPYQRLWSAEFNPDGAFEGGKYLAATDQLSLGIWDTTDGTLLGSLHR
                     ADYDEFGNIMMVSFDPTGRYLVGGSTGGPVWVADLERVVAGETMVDALVFNRQAHTGA
                     APVPAINANGIVASAGYDDMVRLWDLHSEDLILEFESELGVPVVRFSSGGTELLYPHG
                     PSMRRMPVDPYQLRALAGELLTRDFLPDECVRYAAAERCEGS"
     gene            complement(2313..2810)
                     /locus_tag="VFZ80_04915"
     CDS             complement(2313..2810)
                     /locus_tag="VFZ80_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018237642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HEX6286805.1"
                     /translation="MSAALWALETENVDEALRFAPEVAEEEIRIAFAKAVEARKAGDV
                     AKDVADLYFAETVVRLHRAGEGAAYTGLKPGGLDVGPVIPIAERAIETGSVDELSSLL
                     AETLQHQTKRRFDHVMELKAGSHLGLAESRAYTGSMLGLQVWAHKLYLAMQAAAHERH
                     DEAHE"
     gene            3126..>3517
                     /locus_tag="VFZ80_04920"
     CDS             3126..>3517
                     /locus_tag="VFZ80_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286806.1"
                     /translation="MRRLGHSLSVGISFGVTSGIITTVGLLVGLSAGTNSRAAVLAGI
                     VTIAVADALSDALGIHISEENEGVHTSREVWEATAATFASKMIMALTFVIPVLLLPLG
                     TATIVAVVWGALSLTALSFDMGRRQGIDP"
BASE COUNT          651 a         1091 c         1073 g          702 t
ORIGIN      
        1 ggcggtgggt gcgatggcac aggcgatcca agactggcgt ctggtctccc gatttccggc
       61 aggctctgtc gtctttccag acgtatcgcc cgacggatcg caggtggcgg tctcatccaa
      121 gaacactccg agcgatgtag acctattcgc cgctgacgga tccttgatcg ggaccctgca
      181 tggtccaaac gatcccacgg tggttgcatg gcaggtggac ttcagcccgg atgggcgaac
      241 catcgccgtg atctatgccg gcagcgacaa tatcgacgaa ttccggcaac tacccgatgg
      301 ctttccggat ctcaggacct tcgacctgac gaccatgtca accatcctcg agctggacac
      361 cgaccatggt tggcgataca tctcacatga cctcgacggc ggccggctcg ccctctcctt
      421 tgaccgagaa atacgcatac tcgacgctgt cgatggaacc gagatctcca ggatcgaagc
      481 gcggtcgacg gtcggtcgcg cccagttcct cgacgacgac accgttttgg tgcccgtcgg
      541 gggctccggc ttcaccagct tcgacccgaa cggcggctcg gaggtcgacc atctcgaagt
      601 ggctggactg attccggcta tcacagctat caatgacctg cgaacccacg tcgcctatcg
      661 agagggcgag caggtgcgtg tcacggagct gtccaccggt gagacgatct acgaccgcac
      721 cggtgaatcg gtgcagacgc tggccctcga cccggacgcc acgcggctgg cccactccgg
      781 attcgacccg aacatcacca tcgatgccct tgatgatgcg gacatggggt tggagctgac
      841 cgggatactg gagaacgtgt tttcgctgac cttcatgggg gagagccagc tcctctccta
      901 tggagaggat gccttgctct ggaacgtgtc accagaaggc gtgagcgagc tgggaggcat
      961 ccccttgcag gagctgcaat gggggttcca gatatcaccc gacgagcgtt ggctgagcta
     1021 ctacgtgtcg accaacagcg gtatcccgcc ggccgacccg gtcgacggtc tgaggtggat
     1081 cgacctctcc actggtgagg agactgtgat cagccagggg gagatcaacc tcgtgccggg
     1141 tgggtttcga tcgatcaatc cggatttcac gatggtcggc gctctcacgc aaaatggcga
     1201 gtcgacgctg cgaggtttgc cggcatggga tgtgatcagg gaattcgatg catgccgggg
     1261 tctcctcgcg atcacacccg acaacaccat gggcgtgttg tcgggatggt cctgtgacac
     1321 cccggtcccc gaggccgctc acagcgtggt actcgacctc gagtcgggag acgaaatctt
     1381 cccgcttccc taccagaggt tgtggtcggc cgagttcaat cccgatggag cgttcgaagg
     1441 cggaaagtat ctagccgcaa ccgaccagct cagcctcgga atctgggata ccaccgacgg
     1501 aacgttgctg ggcagcctcc atagagccga ctacgacgag ttcgggaaca tcatgatggt
     1561 gagtttcgac cccaccggac ggtatctcgt cggaggatcc acgggcggcc cggtttgggt
     1621 cgccgacctg gagcgagtcg ttgccggtga gaccatggtc gatgctctcg tcttcaatcg
     1681 tcaggcccac accggggccg cacccgtgcc cgccatcaac gccaatggaa tcgtggcctc
     1741 ggcgggctac gacgacatgg tccggctctg ggatctccac tctgaggacc tgatcctcga
     1801 attcgagtcg gagctcgggg tcccggtggt ccggttctct tctggcggca ccgagctcct
     1861 ctatccacac gggcccagca tgcgacgcat gcccgtcgat ccgtaccaac tacgtgctct
     1921 tgccggtgag ctcctcaccc gtgactttct tcctgacgaa tgcgtgcggt atgcggctgc
     1981 ggaacgctgc gaaggctcgt aggtacagct tgcagtggga gcttcgacac gacgggtgga
     2041 gtcactccga gccacctctc tagaagagtc cgaagttggc gttgacctcg cctctgctgc
     2101 agccctggcg tagccggggt gcgccaaggg cgctcagaca gctcaccggc gggcggcaag
     2161 gtctcgctat gactcgtggg ccatagacaa gccgttgccg gcaggggtcc tccgcgtctc
     2221 acggccaaac agtgagaatg cctgcagccc gggagagtgc caccccatga ttgccgatcc
     2281 ggacaaggag gagccgagac gggattcttt ccctattcgt gagcctcgtc atggcgctca
     2341 tgtgcggcgg cctgcatagc cagatacaac ttgtgtgccc agacctggag tccgagcatg
     2401 gatccggtgt aggcacggga ctcggctagc cccagatgtg aacctgcttt gagttccatc
     2461 acgtggtcga agcgacgttt tgtctgatgt tgcagcgttt cagccagaag agagctcagc
     2521 tcgtcaaccg aaccggtttc gatagctcgc tccgcaatcg gtatgactgg gccgacgtcc
     2581 aatccgccgg gttttagacc ggtgtatgcg gctccctccc cggctcggtg caatctgacc
     2641 acagtctcgg cgaaatacag atcggcgaca tccttggcga cgtcaccggc ctttcgagcc
     2701 tcgacggctt tagcaaaggc gattcgaatc tcctcttcgg ccacttcggg tgcgaatcgg
     2761 agagcctcat cgacgttttc agtctccagg gcccacaggg ctgcagacac caccggaccg
     2821 tccttggaat cacaatgcgg gggcatgaac catcacctct ttcaccggaa ccgcgggcag
     2881 acccgctccg aatctcttat ggacatcagt gttgtgcacg gccagcacgc tcaagacctt
     2941 catgttccac cgaccggggc acagctgtcg ccgtggtttc ttcaacccat cgcctaaacc
     3001 agaagagtga acggcgccag ggtcaaaggt cccaccgtct cgacggagca accagccgag
     3061 acaggatgcc ctcccatggg ttgggacaat ctccccaagc ttggcttgtg ccaggcttgt
     3121 tggagatgag acgccttggt cattcactgt cggtcgggat ctcgttcgga gtgacgtctg
     3181 ggatcatcac cacggtcggc ctcctcgtgg gactgtcagc gggcaccaac tcgcgggcgg
     3241 ccgtgctcgc aggcatcgtg accatcgccg ttgcggacgc cctctccgat gcgctcggca
     3301 tccacatctc ggaggaaaac gagggtgttc acacctctcg tgaggtgtgg gaagcgaccg
     3361 ctgcgacctt cgcgtccaag atgatcatgg ctctcacgtt cgtgatcccc gtgctgcttc
     3421 tcccgcttgg tacggccacg atcgtcgccg tcgtctgggg ggcgctgtcg ttgactgcgt
     3481 tgagtttcga catgggtcgt cgtcagggga tagatcc
//
LOCUS       DASUTA010000246         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_460203, whole genome shotgun sequence.
ACCESSION   DASUTA010000246 DASUTA010000000
VERSION     DASUTA010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460203"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1505
                     /locus_tag="VFZ80_04925"
     CDS             150..1505
                     /locus_tag="VFZ80_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX6286807.1"
                     /translation="MFIARQTDELCTFAVVNPGSAVERVSEALAKAHASQDTLNAFIS
                     IDDERAMDRAAEVDRRIGAGEDVGPLAGVPVALKDIIEHEGRVTTNGSSFFAEVAKTT
                     ATCVRRLEDAGAIIIGRTNLHEWAFGFNSENEHWGAVRNPWDPSTSAGGSSGGSGAAV
                     AAGITPIAIGTDTGGSVRVPAALCGTYGLKVTYGRIPLDGVFPLVSSIDTVGPLADSL
                     ENIELAYRAMSLDGTESPAPTGLRIGIPEPWYRDAPIEDDIRITFETVVTAFNELGHE
                     VHGMEMPDAVPSRQLIDAIGREVTAAHREYRERGESYGSAVEARIEVAESVTPEAAEA
                     ARQWQAMIRSRFSDAFATVDLLVTPTCGVRRKVIGEEDIGGKSHRTVLSYFSALVNHA
                     LHPALALPIQDSGRPPASLQVIGPLQSEVPLIEFGKTLESAGITGFTPAPANSPITGG
                     G"
     gene            1710..>2383
                     /locus_tag="VFZ80_04930"
     CDS             1710..>2383
                     /locus_tag="VFZ80_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX6286808.1"
                     /translation="MQLAGNRFTDASALFGNDLATLPSFPAEIRAPAGTLPGVSSFQV
                     QIADFDILTAGDAPDCLVAMNPAALMKNFKDLRQGGTLIVNTDAFDERNLAKAGYESN
                     PLEDDSLIGYRVIKAPMDELTKEAVKDTGVSGRNVLRSKNFFALGLMAWLFHRPTEPT
                     IEWVEKKFSKKPEVAAANKAAFQAGYTFGVATEATKNTFEVKPAALREGTYTNVNGNT
                     ALAWGL"
BASE COUNT          478 a          780 c          729 g          396 t
ORIGIN      
        1 atgaaacgga gtggttcaca ctgcgcactg agtccggcca ggtcatgaac gtgccaccgg
       61 gtcagtcctg ggtgtcgatc gtcccctcca atcggggtct gtccatcacc gagtgaccac
      121 gtcctacgtg caacgaacac ggtataggcg tgttcatcgc acgacagacc gatgagttgt
      181 gtacgttcgc agtcgtgaat ccgggcagcg cggtggagag ggtcagcgag gcgctggcga
      241 aagcgcacgc cagccaggac acgctcaacg ccttcatctc gatcgatgac gagcgggcga
      301 tggatcgggc cgccgaggtc gaccgtcgca tcggagccgg tgaggacgtc gggccgctcg
      361 caggtgttcc cgtagccctc aaggacatca tcgagcacga gggtcgggtc accaccaacg
      421 gctcgtcgtt cttcgccgaa gtcgcgaaaa ccaccgctac ctgtgtcagg cggctcgagg
      481 acgccggagc aatcatcatc ggacggacca acctccacga gtgggccttc gggttcaact
      541 ccgagaacga gcattggggc gcggtgagaa acccgtggga cccctccaca tccgcaggtg
      601 gctcgtcagg ggggtcggga gcggccgtgg ccgcaggcat cacgcccata gccatcggca
      661 ccgacaccgg tgggtcggta cgcgtgcccg cagccttgtg cggaacctac ggcctcaaag
      721 tcacctatgg gcgaatacct ctcgacgggg tgttccccct ggtctcctcg atcgacaccg
      781 tcgggccact ggccgactcg ctggagaaca tcgaactggc atacagagcc atgtctctcg
      841 atggcaccga atcgcccgcc cccactggtc ttcgcatcgg cattcccgag ccgtggtacc
      901 gggacgcgcc gatcgaagac gacatcagga tcaccttcga gacggtcgtc acggcgttta
      961 acgaattggg acacgaggta catggcatgg agatgccgga cgcagtgccc agccgccagc
     1021 tgatcgacgc cattgggcgt gaagtgactg ctgctcaccg cgagtaccgg gaacgaggcg
     1081 agagctacgg gtcggcggta gaggcccgca tcgaggtggc agagagtgtc acgcccgagg
     1141 cggccgaggc ggcacgtcag tggcaggcga tgatcaggtc gagattctcc gacgccttcg
     1201 ccacggtgga cctgctggtg actccaacgt gcggcgtgcg ccgcaaggtc atcggtgaag
     1261 aggacatcgg aggcaagagc caccggaccg tcctctccta tttcagcgcc ctcgtcaacc
     1321 atgcccttca tcccgcactg gcgttgccga tacaggattc agggcgacca ccggcttcac
     1381 tacaagtgat tggaccgctc caatcggagg tccctttgat cgaatttgga aagaccctgg
     1441 agtccgccgg aatcaccgga ttcacgcccg ctccggcgaa ttcacctatc acaggtggtg
     1501 gatagacttg gatgtacaag acatgaaccg ggcggtcagc gtctccgaca ccagcgaggg
     1561 gcaccggccg cgcgcaaacg ctagctttca aaccatcaag gatgtgaccc caagtggctg
     1621 atgaagtcgc caccgcagac ccctcctcca ctcccaccga agagctcgac caactcgtga
     1681 tccgattcgc cggcgactcc ggcgacggca tgcagctcgc cgggaaccgc ttcaccgacg
     1741 cctcggcttt gttcggcaac gacctggcga ccctccccag tttccccgca gagatcaggg
     1801 caccggccgg gactctcccc ggagtgtcca gtttccaggt tcagatcgcc gacttcgaca
     1861 tcctcaccgc cggcgacgct cccgattgcc ttgtggccat gaacccggcc gctctcatga
     1921 agaacttcaa ggacctccgc cagggcggga ctctcatcgt caacaccgac gctttcgacg
     1981 agcgcaatct ggccaaggcc ggctatgagt cgaacccgtt ggaggacgac tcgctgatcg
     2041 gctacagggt catcaaggcc ccgatggacg agctgaccaa ggaggccgtc aaagacaccg
     2101 gagtgtccgg gcgcaacgtc ctccgctcga agaacttctt cgctctcggc ctgatggcct
     2161 ggctgttcca tcgccccacg gagccgacca tcgagtgggt cgagaagaag ttctccaaga
     2221 aaccggaggt ggcggctgcc aacaaggctg cgttccaggc cggttacacc ttcggtgtgg
     2281 ccaccgaggc caccaagaac acattcgagg tcaagcccgc cgccctccgc gagggcacct
     2341 acaccaacgt caacggcaac acggctctgg catggggtct gat
//
LOCUS       DASUTA010000247         3747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_979125, whole genome shotgun sequence.
ACCESSION   DASUTA010000247 DASUTA010000000
VERSION     DASUTA010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979125"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1505)
                     /locus_tag="VFZ80_04935"
     CDS             complement(24..1505)
                     /locus_tag="VFZ80_04935"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6286809.1"
                     /translation="MTPTPSRVLSRELLPASIAVFATAALASFQALGLAAALPEIAGD
                     LGDIELLPWVITAYLLTSSLATVVAGPFIDSVGVSRMFRIAVIVFAASGFAAGLSTSM
                     PMLVALRALQGIGGGLVVSTGTAAISLVYPSHLVSRAFAANATVWGVMGVAGPAIAAA
                     MLTFLSWHWVLFINLPLGVVALVAGWRIFPGPFGEREGRLDVIGIVLVAGLTVATLFA
                     VDSLSVESLFWLAGAALATFLYVLHARRTRNPLVRLEHIVPQPYRGLALSTALMLVGT
                     MAMSDYIPLFVRAGRGASSAVTAWSVLPLTVGWTTGATISSRLGDRHSESWVMVLGFM
                     VNIPALALAWVVVTLDAALPLAFLVFFAAGVGVGMSTNAALTLLRAVTDPSKMGRVGA
                     AHLFARNQGFTFGAAVGGAVLLLVVTDRLGDLSLVRDLLAGSTTAGASGDAAAAVESG
                     YATTIAVGLVLSSLGWLSAARLRRHLTEARLAKRGPDAAATPG"
     gene            complement(1542..1766)
                     /locus_tag="VFZ80_04940"
     CDS             complement(1542..1766)
                     /locus_tag="VFZ80_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286810.1"
                     /translation="MTRSIAGGVFVCPIERGQRAFWRYHIRNWFTVFGVKVLPGPVVG
                     TYIECFSCRNTYDPRIVGSPHPALRVDQTD"
     gene            2165..>3747
                     /locus_tag="VFZ80_04945"
     CDS             2165..>3747
                     /locus_tag="VFZ80_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX6286811.1"
                     /translation="MATTRAAWGSRVGKDGWAQPRGFELLERRRFTVLLTVAALMATV
                     LFAGTSDRAAANPASVTIAGSLQSELGCPGDWDPACEVTHLALETEDDVWQAVFTVPA
                     GSWEYKAALNDSWDENYGLGAVRDGPNIPLAASAAGDVKFYYDDGTHWVTDNVNSVIA
                     TVPGSFQSELGCGSDWDPSCLRSWLQDPDGDGVASFSTSLLPPGDYEAKVAIDESWDE
                     NYGAEGVQDGANIPFTVETLGDVVFFSYDEVSHILTIEVTPVTPVDDSELVREPVRHP
                     FVDEVLYFAIPDRFNDGNPSNNCGEFEGPCLPDDTEANILSHGYLPSDKGYYHGGDLE
                     GLRRQLPYLQNLGVGAVWVGPIFQNKVVQPDSTSLFGMSAGYHGYWILDFLEVDPHLG
                     TNEEFTRLVDEAHERGIKVFMDIVTNHTADVIQLEGNAGYRNKTDFPYLDVNDQPFDD
                     SDHAYSGQSDYTFPEVDETSFPYLPVLPDGDEEAKSPAWLNDPLLYHNRGNSSFVGEN
                     SLYGDFFGLDDLWTERREVV"
BASE COUNT          731 a         1196 c         1190 g          630 t
ORIGIN      
        1 taggctcctt cgtccctctc cagctatccc ggtgtggcag cagcgtcggg tcctcgcttg
       61 gcgaggcggg cttcggtgag atggcgtcgc agcctcgccg cactcaacca cccgagtgac
      121 gacagcacca gtcccacggc aatcgtcgtt gcataaccgc tctccacagc cgcagccgca
      181 tcgccggagg cgccagcggt ggtggaaccg gccagaaggt ctcgtaccag gctcaggtca
      241 ccgagacggt ccgtcacaac gaggaggagc acggcaccac cgacggcggc tccgaatgtg
      301 aatccttggt tccgggcgaa gagatgcgcc gcccccaccc gccccatctt cgatgggtcg
      361 gtgacagccc tgagcagtgt cagtgcggcg ttcgtcgaca tcccgactcc gaccccggcg
      421 gcgaagaaga ccaggaaggc caaaggaagg gctgcgtcca gggtgacaac gacccaggcc
      481 agggcgaggg cggggatgtt gaccatgaac ccgaggacca tcacccagga ctcggaatgt
      541 cggtcgccga gacggctcga gatggtggca ccggtggtcc acccgaccgt cagcggcagc
      601 accgaccagg cggtgaccgc cgaggaagcc ccgcgacccg ccctgacgaa caacgggatg
      661 tagtcgctca tcgccatcgt tcccacgagc atcaaggcgg tggagagggc aagaccccgg
      721 tatggctggg ggacgatgtg ctccagcctg accagagggt tcctcgttcg ccgggcgtga
      781 agcacataca ggaaagttgc cagggcggca cctgccagcc agaaaaggga ctccactgac
      841 aaagagtcga cggcgaacag tgtggcgacg gtgagcccgg cgaccaggac gatgcctatc
      901 acgtcgagtc ggccctccct ctccccgaat gggccgggga agatgcgcca tccggcgacc
      961 aaggcgacga cgcctagtgg gaggtttatg aagagcaccc agtgccaact caagaaggtg
     1021 agcatcgccg cggcgatggc cggcccggcc actcccatca ccccccacac ggttgcgttt
     1081 gccgcgaacg cccgactgac cagatgcgag gggtagacca gggagatcgc ggcggtgccc
     1141 gtcgagacaa cgagaccacc cccaatgcct tgcagggcac gaagtgcaac gagcattggc
     1201 atcgaggtcg ataatccggc ggcgaagccg ctggcggcga acacgatcac tgcaatccgg
     1261 aacatccggg agacgccgac gctgtcgatg aacggcccgg cgaccacggt ggccagactc
     1321 gacgtgagga gataggcggt gatcacccag gggagcagtt cgatgtcacc caggtctcct
     1381 gcgatctcgg gaagggcggc cgccagaccc agagcctgga acgaggccaa agcggccgtg
     1441 gcgaacaccg ctatggaggc gggaagcagt tcccgggaga ggacccggga cggggtcgga
     1501 gtcacttgcg ggcagcgtag atcgacccgg gctgagcgat gtcagtcggt ctggtcgact
     1561 ctgagcgccg ggtgtggcga gcccacgatt ctcgggtcgt aggtgttccg gcagctgaag
     1621 cattcgatgt aggtgccgac caccggaccg ggcaggactt tgactccgaa cacggtgaac
     1681 cagttcctga tgtgatagcg ccagaaagcg cgctgacctc tctcgatcgg gcagacgaag
     1741 accccgcccg cgatcgacct tgtcacgctt cgacgtccga ggatgcttcc gactctgacc
     1801 tctgcgtgag cctggctgac ccgttgcatg ggagatcatc ggcagattcg acgttcgggg
     1861 aagtgggtcg gcagaaccag tccgtccggt tcattcctgg ttcggctcat tcccatagct
     1921 cccgtcgaca ggccactggc agtcgtggtc gactctttca cagatcgttc tgatcgaccg
     1981 aggcaccagc gtccaggtca gcctgtgctg cttcgaggag gtcgagcatt cgccgggggg
     2041 tgtcagggtg gctgtccagt cgtttgcgaa cacaacggag cgtctctcgg aagtcctctc
     2101 tgccacgacg tgctgaccga aggccaatcc gacttggagt gactgttcga cgttggcgca
     2161 cgagttggcg accacacgcg ccgcgtgggg gagtagggtc gggaaagacg gctgggctca
     2221 accaaggggg ttcgagttgc tggaacgccg acgcttcaca gtccttctca ccgtcgccgc
     2281 gctgatggcc acggtgctat tcgccggaac gagtgataga gcggcagcca acccggcgtc
     2341 agtgaccatt gccgggagcc tgcagtcgga gttgggctgc cccggcgact gggacccggc
     2401 atgcgaggtc acccatctcg ctctcgagac cgaggacgac gtttggcagg ccgtgttcac
     2461 cgttcccgcc ggatcctggg aatacaaagc ggctctcaat gactcctggg acgagaacta
     2521 tggcctgggc gcagttcgtg acggaccgaa catccccctg gccgcgagtg cagccggcga
     2581 cgtcaagttc tattacgacg atggcaccca ttgggtgacc gacaacgtca actctgtgat
     2641 cgccaccgtc ccgggcagct ttcagagcga gctggggtgt ggctcggact gggatccgtc
     2701 ctgtctccgc agctggcttc aggaccccga cggtgatggg gtcgccagct tctccacctc
     2761 cctcctcccg cccggtgact acgaggcgaa ggtggccatc gacgagtcgt gggacgagaa
     2821 ctacggcgct gagggagtgc aggatggcgc caacatcccc ttcaccgtcg agaccctggg
     2881 cgatgtggtc ttcttcagct acgacgaggt gagccacatc ctgaccatcg aagtcacccc
     2941 cgtcactccc gtcgacgact cggagttggt tcgggaaccg gtccgccatc ccttcgtcga
     3001 tgaggtcctc tacttcgcca tccccgatcg cttcaacgat ggcaaccctt caaacaactg
     3061 tggcgagttc gaaggaccct gtctgcccga cgacaccgaa gcgaacatcc tcagccatgg
     3121 ctacttgccg agcgacaagg gttactacca cggtggcgat ctcgagggtc tccgacgtca
     3181 gctcccctat ctgcagaacc tgggagtggg agccgtctgg gtcgggccga tctttcagaa
     3241 caaagtggtt cagcccgact ccacgagcct gttcgggatg tcggccggct atcacggtta
     3301 ctggatcctc gatttcctcg aggtcgaccc tcacctgggc accaatgagg agttcacccg
     3361 actggtcgac gaggctcatg agcggggcat caaggtgttc atggacatcg tcaccaatca
     3421 caccgccgac gtgattcaac tggaaggcaa cgccggatat cgcaacaaga cggacttccc
     3481 ctacctcgac gtcaacgatc aaccgttcga cgattcggat cacgcctact caggtcagtc
     3541 cgattacacc ttccccgagg tcgacgagac ctcgttcccc tatctccccg tcctccccga
     3601 tggcgacgag gaggccaaga gccccgcatg gctgaatgac ccgctgctgt accacaaccg
     3661 tggtaattcg agcttcgtcg gcgagaactc cctctatggc gacttcttcg ggctcgacga
     3721 cctgtggacc gagcgccgcg aggtggt
//
LOCUS       DASUTA010000248         2045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_591523, whole genome shotgun sequence.
ACCESSION   DASUTA010000248 DASUTA010000000
VERSION     DASUTA010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591523"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(279..396)
                     /locus_tag="VFZ80_04950"
                     /pseudo
     CDS             complement(279..396)
                     /locus_tag="VFZ80_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568715.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            685..1704
                     /locus_tag="VFZ80_04955"
     CDS             685..1704
                     /locus_tag="VFZ80_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX6286812.1"
                     /translation="MARPLRLQFPGGIYHVMNRGNNRQKIFLDDADKALFLAFVIEEI
                     ERCGWKLHDYALMDNHFHLLIETPEPNLSTGMHRLESTYAQQFNKRHGRTGHLFGGRY
                     KSFLVEGGGYLMEVARYIVLNPVRAGMVAHPRDYAWSSYRAKAGYEGAPPWLTMSGLY
                     HFDRDLEKAKAGYREFVDAAIGNPEIETDFLSRIVGQIVFGSGQFVQTIQAMIDSEER
                     STEHPRYQRDIGRPPVSAIMAAVCKRLEVTPEELRERRGHLSRMVFATLGYWEGLRTL
                     REIAQELGLRSYAHVSNLVRRCRRECNKDPVLKRIVEECRAAAQAHAPPLPEHYRSRD
                     PLPVW"
BASE COUNT          403 a          630 c          620 g          392 t
ORIGIN      
        1 gggtttcatt ttttgaaatg gaaagttgcc ggccgcacaa aggtgtggga ccgcgccgga
       61 agcgccacca ccggtgacga ttcgacccca gtccgcgcgt cccggaagga cgccagaggg
      121 gcgcaacaga ctcttctctc cctctccccg caagagacgc cggatagagt tcggagaggg
      181 tcggggtgag gggcccgagc cgaagccagg acggtccgac cgcgaagctc acgagaccgt
      241 gtctcgcaaa atccaccatc ctggtggccc ctcactcgct acgcgtacat gagacggtca
      301 cgtggcgtag gaatctcacg tccgagctca cgagcagtct cgatccactc ctcgaacgat
      361 ccgggcattg ccgacggctt cctcggacat tcggcccatt cggggcccat tcgccattcg
      421 gggtcacgtc ttcattcgcc attcggggtc acgtctttgg ccggcgatat tagcctgatc
      481 tttttcgccg atgtctactc attgtcacca tcaccagggt caggtctttg actgacgatt
      541 caggccgagc tgggctcgcc gatgtctact ccttgtggag tcaccgcctt cgagcttcca
      601 ttccgaggag gttttctgcc gaatttccac ctgtttcgtc taacggcttg acctcagcag
      661 agagccgagg gagcctcggt gatcatggca agacctctga gattgcagtt tccgggcggc
      721 atctaccacg tcatgaaccg aggcaacaac cggcagaaga tcttcctcga cgatgcggac
      781 aaggctctgt ttctcgcgtt cgtcatcgag gagatcgagc gctgcgggtg gaagcttcac
      841 gactacgcgt tgatggacaa tcactttcat ctgctcatcg agactccgga gccgaacctc
      901 tctacaggca tgcaccggct cgagtccacc tatgcccagc aattcaataa aagacacggg
      961 cgcactggac atctgttcgg cggtcgctac aagtcctttc tggtcgaggg tggtggctat
     1021 ctgatggagg tggcccgcta catcgttctc aatccggtgc gggccgggat ggtcgcacat
     1081 ccgcgggact acgcgtggtc gagctatcgg gcgaaggcgg gatacgaggg tgctcctcca
     1141 tggttgacga tgagcgggct ctaccatttc gatcgggacc tcgagaaggc gaaagcaggc
     1201 tatcgggagt tcgtcgacgc ggcgattggc aatcccgaga tcgagacgga cttcctgagc
     1261 aggatcgtcg gccagatcgt gttcggaagt gggcagttcg tgcagacgat ccaggcgatg
     1321 atcgactccg aggagcggag caccgagcac ccgcgatatc agcgggatat cggacggccg
     1381 ccggtgagtg cgatcatggc ggcggtgtgc aaacggctcg aggtcacacc cgaagagctg
     1441 cgagagcggc gtggccatct ctcacgcatg gtgttcgcga cgctcggcta ctgggagggg
     1501 ctgcggacgc tgcgggagat tgcgcaggag ctcggcctgc ggagctatgc gcacgtgtcg
     1561 aacctcgtga ggcgctgccg gcgagaatgc aacaaggatc cggtcctcaa gcgaatcgtc
     1621 gaggaatgcc gcgctgccgc ccaggcgcac gcgccaccac tccccgagca ttaccggtcc
     1681 cgggatcctc tcccggtctg gtagcacccc gatccgccaa caactcttct cacccgtagc
     1741 gatttagatg aagtcgtggg ggagctgcgt cccgaacggc gcggaacagg cactgagcaa
     1801 cgcgacgagt ctaaattcac cactccacca ccgagaccga gcccgagtca cgcccaaaca
     1861 gcgaggcatt catcgccctg gcggccgccc aatcaccgcc caaaacgggg ccaggtcttt
     1921 caatgtcgtc tcgatcaccg ccaatcaaag acctgtcccc tattggctat tggctatgag
     1981 ccccaggatt ttcgaggtcc atgtcaggag ccctgaagtt gagctctcgc agccggaccc
     2041 gtcgc
//
LOCUS       DASUTA010000249         2732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1627761, whole genome shotgun sequence.
ACCESSION   DASUTA010000249 DASUTA010000000
VERSION     DASUTA010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627761"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VFZ80_04960"
     CDS             complement(<1..554)
                     /locus_tag="VFZ80_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6286813.1"
                     /translation="MDFSYSDRVEDFRSRLLDFMDGHIYPSEQIFAQQMEEAGDPHHH
                     PPVLEELKSEARSRGLWNLFLPDPEDGAGLSVLEYAPLAEITGRSVQIAPEALNCSAP
                     DTGNMEILHMFGTPEQKELWLHPLLEGEIRSCFSMTEPDVASSDATNIRTRIERDGDE
                     YVITGRKWFSSGAASSRCKLSIVMG"
     gene            complement(567..1325)
                     /locus_tag="VFZ80_04965"
     CDS             complement(567..1325)
                     /locus_tag="VFZ80_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX6286814.1"
                     /translation="MPVLRFRLFNMPVTVRGSFIVIAALIGFTGVGDAAQTVAWVGIV
                     FVSILVHELGHAFTARAFGSEVSIELNGLGGLTRWATSQGQLTPGRRALIAAAGSATG
                     LLFGGAVWLVAAQFGPYRSLTAFILQSLVYVNVFWGLLNWLPIRPLDGGHLLESLLEK
                     IAPRRAETIARVIFTATAAAALALAIWQNMIFIAVLAGWLLLSEFSFGRESGVPAVLP
                     TFSYDDEIEADDGDDRPHEPDEVIDPEESGELPR"
     gene            1352..2701
                     /locus_tag="VFZ80_04970"
     CDS             1352..2701
                     /locus_tag="VFZ80_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020217426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6286815.1"
                     /translation="MHDDLKSAVAADMPRLKELLATLIRMESVSADGYDPAGVRTAGE
                     TIVKKLDEAGFDNARLLESSTGHPAVFAEKPGPAGAPTVLLYAHYDVQPPGPIEEWET
                     GPFEPFEKDGRIYGRGASDDKAGIVMHLGAIAAHAENGLPVGVQLFLEGEEEAGSVGL
                     PEILEKHSDLLAPDVIVIGDGGNWEEGTPAYISSLRGIVAVKLELRTLESAVHSGQYG
                     GVVPDALMALSRLLATLHDDEGNVAVPGLVSEEVEDVPEVSEQRARAETGAIEGLELI
                     GMGTIPSRLWSRPALSILAIDAPGTTEAINQLVPVARAKVSMRIPPGQDTEAALKALK
                     DHLMANVPWGAELSFFHEEKGDATVLGSDNFAVDIWREAFLEAFGSEAVEMGAGGSIP
                     FIATFSEMFPNAPILVVGAADPKSAFHAPNESQSVADLEKATLSEAIALRLLAERGV"
BASE COUNT          506 a          852 c          921 g          453 t
ORIGIN      
        1 cccatgacga tcgagagctt gcagcgcgag gacgccgctc cagagctgaa ccatttgcgc
       61 ccggtgatca cgtactcgtc accgtcgcgc tcgattcggg ttcggatgtt ggtggcgtcg
      121 gagctggcca cgtcgggctc ggtcatcgag aagcaggatc tgatctcgcc ctcgagcaac
      181 ggatggagcc acagctcctt ctgctcggga gtcccgaaca tgtggaggat ctccatgttg
      241 ccggtgtcgg gagccgagca gttgagcgcc tcgggggcga tctggacact acgcccggtg
      301 atctcggcca gaggcgcata ctcgagcacc gacagcccgg ccccgtcttc gggatcaggg
      361 aggaacaggt tccacagacc acggctgcgc gcctcgctct tcagctcctc gagcacgggg
      421 ggatggtggt gtggatcgcc cgcctcctcc atctgctggg cgaagatctg ctcggagggg
      481 tagatgtggc cgtccatgaa atcgagtagt cggctccgga agtcctccac ccgatcgctg
      541 tatgagaaat ccatcgtgac gggaagctag cggggtagct ccccggattc ctccgggtct
      601 atgacctcgt cgggctcgtg gggccggtcg tctccatcgt cggcctcgat ctcgtcgtcg
      661 taggagaagg tgggcaacac ggccggaacc ccgctctcgc gcccgaagct gaactcggag
      721 agcagcagcc agccggccag tacggcgatg aagatcatgt tctgccagat ggcgagggcg
      781 agagcggcgg cggccgtcgc ggtgaagatg acgcgggcga tcgtctcggc ccgtctcggg
      841 gcgatcttct ccagcaacga ctcgaggagg tggccaccgt cgagtgggcg gatgggcagc
      901 cagttcaaca gcccccagaa gacgttgacg tagaccaggc tctgcaggat gaatgccgtc
      961 agactccgat acgggccgaa ctgggcggca accagccaca cggccccgcc gaataggaga
     1021 ccagtggccg acccggccgc agcgatcaac gcccgtcgcc cgggggtcaa ctgtccctgt
     1081 gacgtcgccc accgggtgag accaccgagc ccattgagct cgatcgacac ctcggatccg
     1141 aaggcccgtg cggtgaaggc gtggccgagc tcgtggacga ggatggagac gaacacgatg
     1201 ccaacccatg ccacggtctg ggcggcgtct cccacaccgg tgaagccgat caaggcggcg
     1261 atcacgatga acgagccccg gaccgtgacc ggcatgttga acagacggaa cctgagcacg
     1321 ggcacatgtt ggcaccggta cgctcctggg catgcatgat gacttgaagt cggcggtcgc
     1381 cgccgacatg ccccgtctga aggagttgct cgcaaccctc attcggatgg agtcggtgtc
     1441 cgccgacggc tacgaccccg ccggagtgcg aaccgccggg gagacgatcg tcaagaagct
     1501 cgacgaggcc ggtttcgaca atgcccggct cctggagtca tctaccgggc atcccgccgt
     1561 gttcgcggag aaacccggac cggcgggggc tccgaccgta ttgctctacg ctcactacga
     1621 cgttcagcct cccggaccga tcgaggagtg ggagacaggc ccgttcgagc cgttcgagaa
     1681 ggacggccgt atctatggac gcggggccag tgatgacaag gcgggcatcg tgatgcatct
     1741 gggggcgatc gccgctcacg cagagaacgg ccttcctgtc ggcgtgcagc tcttcttgga
     1801 gggtgaagag gaagcaggct cggtcggtct tcccgagatc ctggagaaac actccgacct
     1861 gctcgccccc gacgtcatcg tgatcgggga cgggggcaat tgggaggagg ggacaccggc
     1921 gtacatctcg tcactgcgcg gcatcgtcgc agtgaagctc gagctgagga ctctcgaatc
     1981 tgcggtgcac tccggccaat acggcggagt ggtgcccgac gccctcatgg ccctgtcccg
     2041 gcttcttgcc acccttcatg acgacgaggg caacgtggcc gtgccaggac tcgtttcaga
     2101 ggaagtcgag gacgtgccgg aggtgtcgga gcaacgggcg cgggccgaaa cgggagccat
     2161 cgagggtctc gagctgatcg ggatgggaac gatcccttca cgcctctggt cacggcctgc
     2221 gctgtcgatc ctcgccatcg atgcaccggg caccaccgaa gcgatcaatc agctcgtccc
     2281 cgtggcgagg gcgaaggtga gcatgcgcat accccccggg caagacaccg aggccgcgct
     2341 gaaggcgttg aaggaccatc tcatggcaaa cgtgccatgg ggagccgagc tgtcgttctt
     2401 ccatgaggag aaaggtgatg cgacggtcct cggctctgac aacttcgcag tcgacatctg
     2461 gcgcgaggca ttcctcgagg ctttcggctc ggaggcggtg gagatgggtg cgggaggatc
     2521 gatccccttc atcgccacct tcagcgagat gttccccaat gcgccgatcc tggtggtggg
     2581 cgccgccgac cccaagagcg ctttccacgc accaaacgag agtcaaagcg tcgccgatct
     2641 ggagaaagcg accctctccg aggccatcgc gctgaggttg ctcgccgaac ggggtgtctg
     2701 agccgcggtt gcccgtgcac catgtctcgg aa
//
LOCUS       DASUTA010000250         2325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_849640, whole genome shotgun sequence.
ACCESSION   DASUTA010000250 DASUTA010000000
VERSION     DASUTA010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849640"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VFZ80_04975"
     CDS             <1..1011
                     /locus_tag="VFZ80_04975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6286816.1"
                     /translation="ETLVGLIGAGGEGSARKLIERLQVALDAAGITLFDTVGGTLTPV
                     TSAGDHPPDTVEAADRTIGVAGGALVAIVGGPLDPVDERLLAATATELAAVIDRAEMA
                     NHVAEVDALERANELRTALLRAVSHDLRSPLSAIQASVTSMLQEDVQWPPEDQTEFLQ
                     TISEETERLNRVVSDLLDAGRLQTGTLQVQKRPTGIEDVVSTVATAFRAQGHRLSIDT
                     EIELPDVSTDPVLLERIVENLVRNALDHSPAETPVRVTAGVVGDRIDLRIIDRGPGID
                     PNTRQVIFEPYRTLDDSQMAGVGLGLSVAQGLADALGHQIAIDDTPGGGTTMILSVEI
                     HR"
     gene            1008..1706
                     /locus_tag="VFZ80_04980"
     CDS             1008..1706
                     /locus_tag="VFZ80_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016910140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6286817.1"
                     /translation="MTRVLVVEDDRALVRALANNLKVRGYNVDVATDGISAVKRAADK
                     RPDLVIVDLGLPGLDGIEVIRSIRGWTEIPIIVLSARGSEADKIEALDAGADDYVAKP
                     FGMGELLARVRAALRRATPSDSVATVETPHFSIDLASKKVRVGGEEVHLTPTEWGMLE
                     MLVRNPGSLVSQTMILREVWGPTYEDETHYLRSYIAQIRRKLEPEPSRPRYFITEAGM
                     GYRFEPAKGSKGQV"
     gene            complement(1697..>2325)
                     /locus_tag="VFZ80_04985"
     CDS             complement(1697..>2325)
                     /locus_tag="VFZ80_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286818.1"
                     /translation="SRITVILADRVRMRSILAPFAHRHSLAIKSRLLFEPGVVVTDLN
                     VLRRSRRSRLQAVPVGRIEQVVLISDMTRPIREALTYAQSLGPMVTAVHIDVDSAQRE
                     RLEQQWQKAGYEFELEVIASPFRSIVDPLVAYLKERRRAATPGTLISAVIPEFVVPGR
                     VTQLLHNQTGLAIKGVLAAEPGIAVTSVPFHLLPESKQRDQTRKTGQT"
BASE COUNT          429 a          750 c          755 g          391 t
ORIGIN      
        1 gagacactcg tagggttgat cggcgccggc ggcgaagggt ccgctcgcaa gttgatcgaa
       61 cgacttcaag ttgctctcga cgcggccggc atcaccctgt tcgacacggt cggcggcaca
      121 ctcacaccgg tcaccagcgc aggggaccac ccccccgaca cggtcgaggc cgccgaccga
      181 acgatcggcg tggcaggcgg ggccctcgtt gccatcgtcg gcgggcctct cgaccccgtg
      241 gatgagcgcc tcctcgcggc aacggccacc gagcttgccg cggtcatcga ccgcgccgag
      301 atggccaatc acgtcgccga ggtggacgct ctggagaggg ccaacgagct cagaaccgcg
      361 ctgctgcgcg ccgtatccca cgatctgcgc agcccactct cagcaattca ggcctcggta
      421 acgagcatgc tccaagagga cgtccaatgg ccacctgaag accagacgga gttcctccag
      481 accatcagtg aggagaccga gcggctgaat cgtgtcgtct ccgatctgct cgatgcgggt
      541 cggcttcaga ccgggacact gcaagttcag aagcggccca ccgggatcga ggacgtggtg
      601 tcgactgtgg cgacggcttt ccgcgcacag ggtcaccgac tctcgatcga caccgagata
      661 gaactccccg acgtctcgac cgatccggtg ctgttggagc ggatagtcga gaatctggtc
      721 cgcaatgccc tcgaccactc tccggccgag accccggtga gggtcaccgc cggagttgtc
      781 ggcgaccgga tagacctgag gatcattgac cgagggccgg gcatcgaccc caacacgcga
      841 caggtgatct tcgagcccta ccggactctc gacgacagtc agatggccgg ggtcgggctg
      901 gggttgtccg tggcccaagg tctcgctgac gccctgggcc atcagatagc tatcgacgac
      961 acaccgggtg gcggcacgac catgatcttg tcggtggaga tccacagatg acgcgcgttc
     1021 tcgtcgtcga agacgaccgg gccctggtgc gcgcgctggc caacaacctc aaggtccgcg
     1081 ggtacaacgt cgacgtcgcc accgacggga tctccgccgt caagcgcgcc gcggacaagc
     1141 gacccgatct cgtgatcgtg gaccttgggc tgcccggact cgatgggatc gaggtgatcc
     1201 gcagtattcg gggctggacc gagatcccca tcatcgtcct gtcggcccgt ggcagtgaag
     1261 ccgacaagat cgaagctctc gacgccggag ccgacgacta cgtcgccaag ccgttcggga
     1321 tgggggagct cttggctcga gtgcgcgccg cgcttcgacg ggccacaccc agtgacagtg
     1381 tggcgactgt cgagaccccg catttcagca tcgacctggc gtcgaagaag gtccgagtgg
     1441 gcggggaaga ggtccacctg accccgacgg agtggggcat gttggagatg ctcgttcgca
     1501 acccgggctc tctggtgtcc cagacgatga tcctcagaga ggtctggggg cccacctacg
     1561 aggacgaaac ccactatctg cggtcctaca tcgcccagat ccgacgcaag ctcgaacccg
     1621 agccgtcccg gccccgatac ttcatcaccg aggcagggat gggttatcgc ttcgaaccgg
     1681 cgaagggctc aaagggtcag gtctgacccg tcttcctcgt ctgatcgcgc tgtttgctct
     1741 cggggaggag atggaacggc acgctggtta ccgcgatccc gggctctgcc gccagcaccc
     1801 ctttgatcgc cagcccggtc tggttgtgaa gaagctgtgt gaccctcccg ggcaccacga
     1861 attcggggat cacggccgag atgagagtgc ctggagtcgc ggcacgtcgc ctttccttca
     1921 ggtaggcgac gagaggatcg acgatgctcc ggaaagggct ggcgatcacc tcgagttcga
     1981 actcgtaccc ggctttctgc cactgctgct ccaacctctc cctctgggca ctgtccacgt
     2041 cgatgtggac cgctgtcacc atcgggccga gggattgggc gtaggtcagc gcctcgcgta
     2101 tgggccgggt catgtcggag atgaggacca cttgctcgat gcgacccacc ggcacggcct
     2161 ggaggcgact ccgtctcgag cggcggagga cattcaggtc ggtcaccacg acacccggct
     2221 cgaacagcaa tcgcgacttg atggcaagac tgtgtcggtg ggcgaatggg gcaaggatcg
     2281 acctcatacg gacccggtcg gcgaggatga cggtgatcct cgaat
//
LOCUS       DASUTA010000251         5122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1627811, whole genome shotgun sequence.
ACCESSION   DASUTA010000251 DASUTA010000000
VERSION     DASUTA010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627811"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VFZ80_04990"
     CDS             <1..589
                     /locus_tag="VFZ80_04990"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX6286819.1"
                     /translation="GSTGVLLAHGAGTDQEHPSMSMLREGLAAGGLRVMSFNYPYTER
                     GSKRPDRQDKLLACHLAAADVLSSEVDRLFLAGRSMGGRMGTYLVAEGLSSAGLVLYA
                     YPLHPPGKPEKLRISQFADVAVPMLFFQGTKDTLSRMDLFNEHIVPMENAEVELLEGA
                     GHGYRGGGWNLETITERYVAGTLSWVARLPSGTTE"
     gene            645..1340
                     /locus_tag="VFZ80_04995"
     CDS             645..1340
                     /locus_tag="VFZ80_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX6286820.1"
                     /translation="MSQVSLRAQAGRKPGSRESRRIRRSGDVPAVVYGSDLDPIPVQV
                     DSHDLHVALHTEAGTNAIITLEIEGGDTLTTMARVIERHPFRNEYRHVDFITVDLSQK
                     ITAEVALHWTGTPVGVKEGGVFSPRRTHVQVEVLPTEIPPFIELDVSEVEIGGSLRIE
                     DLPVLESIEYLEDPDAVVMSVTVPAAEIEEPEPEEPELLEGEELAEGEEGEEGADEAE
                     GEGGEEAEEEGGE"
     gene            1351..1923
                     /gene="pth"
                     /locus_tag="VFZ80_05000"
     CDS             1351..1923
                     /gene="pth"
                     /locus_tag="VFZ80_05000"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014926012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX6286821.1"
                     /translation="MVVGLRNPGPDYEGTRHNSGAEVVARAVEAAGESFKRGPSRISG
                     LVAQTGVGDARTLYLLPYTFMNESGSAVRAALDYYGLGPSDLIVVHDDIDLPFGRLRI
                     QVGGGSGGHNGIRSLERALGTKEFSRLKFGVGRPPGSQDPADFVLRPFAKSERAEVEE
                     MIVYAADVIERWLQDRERAQEMAALRGRED"
     gene            1926..3401
                     /gene="glpK"
                     /locus_tag="VFZ80_05005"
     CDS             1926..3401
                     /gene="glpK"
                     /locus_tag="VFZ80_05005"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016692812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX6286822.1"
                     /translation="MADLVGAIDQGTSSTRFMVFDSDANVVAVAQEEHRQILPQPGWV
                     EHDPTEIWDTTRRVIEEALGGASLQASDLAGVGITNQRETTVVWDRATGVPVANAIVW
                     QDTRTADLCSDLGGDEGIDRFRGRTGLPLATYFAGPKIRWLLDENDLTDRAQRGELAF
                     GTIDSWLAWNLTGLHITDVTNASRTLLMDLVTTGWDSDLCMEIGVPTHMLPDIVSSIG
                     EIATCRGPLDGVKLAAILGDQHAALFGQTCFSPGEAKNTYGTGNFMLMNTGTTPVGSR
                     SGLLSTVGYRIEGEDTVYALEGSIAVTGSLIQWLRDNLGIISSASEVEELATSVEDNG
                     DVYFVPAFSGLFAPHWRPDARGVIVGLTRFANKGHIARAALEASAYQSADVLEAMTED
                     SGVALSELRVDGGMTANDALMQFQADLLGVDVVRPSVTETTALGAAFGAGLATGVWAD
                     LDQLRALWSEDARWSPEMAREEAARLHDRWRQAIDRSLGWA"
     gene            3551..4114
                     /gene="pgsA"
                     /locus_tag="VFZ80_05010"
     CDS             3551..4114
                     /gene="pgsA"
                     /locus_tag="VFZ80_05010"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX6286823.1"
                     /translation="MSLNFPNALALFRIAMVIPIMSFTRADQIEGHYTIAAAIFLVAS
                     FTDFLDGYLARRTTGGTVLGAFLDTTADKIMVTGTLLALVSVGRVSIWPAAIIIFREF
                     TVMALRGVVAQRGGLIAPSTWGKLKAFTQYAAIFFAFIRFSEKWGPWYFDQYLMLIAV
                     IATIGSAWGYMSAFWSVVRHHPTDAKT"
     gene            4111..5082
                     /locus_tag="VFZ80_05015"
     CDS             4111..5082
                     /locus_tag="VFZ80_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6286824.1"
                     /translation="MSVAVTGGSGVVGSAVVRHLVDGGEEVWALARSAESSHDLSELG
                     ARVVTGDLFDRDALARLVKGRSRVFHIAGVNEMCSRDPERMWRVNVEGSNRVVAACID
                     AGVERLVHTSSAVAIGEPEGTMGTERSPHRGEFLSEYERSKTVAERMVLDRAAEIDVV
                     FVNPSSVQGPGRSTGTGAIFLAIARGDLPFLVDTTISLVDIDDCARGHLLAAEKGLRG
                     ERYLLSGPVLTIRQAVSMLRRKTGRRSSPRFLHPGVVTAVAPVVGAVAHLVGVQPPLC
                     PESARVLLHGHQYDGSRATRELGLEYTSLEETLDRTLAWFDAEGLLD"
BASE COUNT          885 a         1645 c         1654 g          938 t
ORIGIN      
        1 aggaagcacc ggcgtgttgc ttgcccacgg agcgggcaca gaccaggagc atccatcgat
       61 gtcgatgttg agagagggtc tggccgcagg ggggttgcgg gtcatgtcgt tcaactaccc
      121 ctacacggag cgagggtcga agcggcctga tcgacaggac aagctcctcg cctgtcatct
      181 ggccgcagcc gacgtgctga gttccgaggt cgaccgtctg ttcctggcgg gccggtcgat
      241 gggcggcagg atggggacct atttggtcgc ggagggcctc tcctctgccg gactcgtact
      301 gtatgcctat ccgctgcatc ccccagggaa gcccgagaag ctccggatca gccagttcgc
      361 cgatgtcgcc gtccccatgc tcttctttca aggcaccaag gacaccctga gtcggatgga
      421 tctgttcaac gagcacatcg tgcctatgga gaatgccgag gtcgagttgc tcgagggggc
      481 gggccacggc taccggggag gcggctggaa tctggagacc atcaccgagc gatacgtcgc
      541 cgggaccctc tcctgggtgg ctcggctccc atctggcaca acagaatgac tccggccata
      601 attggcgctc acccctttct cattcgtcca aggagttctt cacaatgtcg caagtaagcc
      661 tgcgcgccca agccggcaga aagccaggct cacgcgagtc gcggcgaata cgccgaagcg
      721 gagacgtccc ggccgtcgtc tacggcagcg acctcgaccc gattccggtt caggtcgact
      781 ctcacgatct gcatgtcgct ctgcacaccg aggcagggac gaacgccatc atcaccctcg
      841 agatcgaagg cggggacacg ctcaccacga tggctcgagt catcgagcgt caccccttcc
      901 gcaacgagta ccgccacgtc gacttcatca ccgtcgacct cagccagaag atcaccgccg
      961 aagtggcgct gcactggacc ggtacccccg tcggtgtaaa ggagggcggt gtgttcagcc
     1021 cccgacgaac ccacgtgcag gtcgaggtgc tgcccaccga gatcccaccg ttcatcgagc
     1081 tcgacgtgtc ggaggtcgaa atcggcggct cgctgcgaat cgaggacctg cccgttctcg
     1141 agagcatcga gtatctcgag gaccccgacg ccgtcgtcat gtccgtgacc gtgccggccg
     1201 cggagatcga agagcccgag cccgaggagc cggagcttct cgagggcgaa gagctggcag
     1261 agggtgaaga aggcgaagag ggtgctgacg aggctgaagg cgagggcggc gaagaggccg
     1321 aagaagaagg cggcgaatag cccggtgcgc atggtcgtcg ggctccgcaa cccggggccc
     1381 gactacgagg gcaccagaca caattccggg gccgaggtcg tcgcacgggc ggtcgaggcc
     1441 gccggggagt ctttcaaacg tgggccttct cgcatcagcg ggctggtggc gcagaccggc
     1501 gtcggcgacg ctcggactct ctatctcctt ccctacacct tcatgaacga gtcgggctcc
     1561 gccgtgcgcg cagctctcga ctactacggg ctcgggcctt ccgatctgat cgtggttcac
     1621 gatgacatcg accttccctt cggtcgtctt cgcatccagg tgggaggggg cagcggcgga
     1681 cacaacggaa tccgctcgct ggagcgagcc ctgggcacca aagagttctc ccgtctcaag
     1741 ttcggagtcg ggcgaccgcc cggttctcag gacccggccg acttcgtgct ccgacctttt
     1801 gccaagagcg agcgagccga agtcgaagag atgatcgtct atgccgccga cgtgatcgag
     1861 cgctggcttc aagacagaga gcgggctcag gagatggctg cgctccgggg ccgagaggac
     1921 tgacagtggc cgatctcgtt ggtgcaatcg accaggggac cagctccacc cggttcatgg
     1981 tcttcgactc cgacgccaac gtcgtggcgg tggcccaaga ggaacaccgt cagatcctgc
     2041 cccaacccgg atgggtggag cacgacccca ccgagatctg ggataccacc cgccgggtga
     2101 tcgaggaggc tctgggcggg gcgtcgctgc aagcctccga tctcgccggc gttgggatca
     2161 ccaaccagcg ggagacgacc gtggtctggg acagggcgac aggggttcct gtggccaacg
     2221 ccatcgtgtg gcaggacacc cgcaccgcag atctctgctc cgatctcggc ggcgacgagg
     2281 gcatcgaccg gtttcggggg cgcaccggcc tgccactcgc cacgtacttc gccgggccga
     2341 agatccgctg gttgctcgac gagaacgatc tcaccgaccg ggcccaacgc ggagagttgg
     2401 ccttcggcac catcgactcc tggctcgcct ggaacctgac tggcctccac atcacagacg
     2461 tgacgaatgc gtcacgcact cttctcatgg acctggtgac caccggttgg gactccgacc
     2521 tgtgcatgga gatcggcgtc cccacacaca tgcttcccga catcgtctcc tcgatcggcg
     2581 agatcgcgac atgtcggggc ccgttggatg gcgtgaagct ggctgcgatc ctcggcgacc
     2641 agcatgcagc cctcttcggc cagacctgtt tctcacccgg tgaggcgaag aacacatacg
     2701 gcacgggcaa cttcatgctg atgaacaccg ggaccacgcc cgtcggctcc agatccggcc
     2761 tgttgtctac tgtcggctat cgaatcgaag gcgaggacac ggtgtacgcc ctcgaaggat
     2821 cgatcgcggt gaccgggtcg ctgatccaat ggcttcgaga caatctgggg atcatctcct
     2881 ccgcatccga ggtcgaagag ctcgccacct cggtggagga caacggcgac gtctacttcg
     2941 tgcccgcttt ctccgggctc ttcgccccgc actggcgtcc cgatgccagg ggagtgatcg
     3001 tggggctgac ccgcttcgcc aacaagggcc acatcgccag ggctgcgctg gaggcttccg
     3061 cttaccagtc cgccgatgtt ctcgaggcaa tgaccgaaga ctcgggcgtc gctctcagtg
     3121 aactgcgagt cgatggaggc atgactgcga acgatgcgct catgcagttc caggccgatc
     3181 ttctgggagt cgacgtggtt cgaccgtcgg tcaccgagac cacagccctc ggcgccgcct
     3241 ttggggccgg tttggccacc ggtgtctggg ccgatctcga tcagctgcgt gctctctggt
     3301 ccgaggacgc ccgctggtcc cccgagatgg cgcgtgaaga ggccgcccgt ctccacgatc
     3361 ggtggcgtca ggccatcgac cggtccctgg gctgggcgtg aaggcatcac ggtccagtcc
     3421 gatgtcaccg gactggatcg gaacacagct ctggtgaggg tgtctgtcca cccctgcgag
     3481 tagacgctca cagaacggtt caaagccccc aggtgacccc ctctgcatat gatcggccgc
     3541 ggagtcgagg atgtcgttga actttcccaa tgcgctcgcg ttgttccgca tcgcgatggt
     3601 gataccgatc atgtcgttca cccgcgccga ccagatcgag ggccactaca ccatcgcggc
     3661 tgcgatcttc ctcgtggcca gcttcaccga ctttctcgac ggctatctcg cccgtaggac
     3721 caccggcgga acggtcctgg gggcgttctt ggacacgacc gccgacaaga tcatggtgac
     3781 ggggacgctc ctcgccctcg tctcggtcgg ccgtgtgtcc atatggcccg ccgccatcat
     3841 cattttcagg gaattcaccg tgatggcgct gcggggtgtc gtcgcccaac gcggggggct
     3901 gatcgcccct tcgacgtggg ggaagctgaa ggccttcacc cagtatgcgg cgatcttctt
     3961 cgccttcatc cgcttctccg agaagtgggg accctggtac ttcgaccagt acctgatgct
     4021 gatagccgtc atcgccacca tcggatccgc ttgggggtac atgagcgcct tctggtcggt
     4081 ggtgcgtcat cacccgaccg acgccaagac atgagtgtcg ccgtcaccgg gggatcgggt
     4141 gtggtgggca gtgccgtggt caggcatctg gtcgacggtg gggaggaggt atgggccctg
     4201 gcacgctcgg ctgagtcgtc gcatgacttg agtgagctcg gagcccgggt agtcacggga
     4261 gacctgttcg accgggatgc cctcgctcgt ctcgtcaagg gtcgaagccg cgtttttcac
     4321 attgccggag tcaatgagat gtgctcgaga gaccccgagc gcatgtggag agtgaatgtg
     4381 gaaggctcga atcgggtcgt cgccgcctgc atcgatgccg gtgtggaacg actcgtccat
     4441 acctcatccg ctgtcgccat cggtgagccc gagggaacga tgggcaccga gcgctcccct
     4501 caccgcggtg agttcctctc cgagtacgag cgttccaaga ccgtcgccga gcggatggtg
     4561 ctcgatcgcg ccgccgagat cgatgtggtc ttcgtcaatc cgtcttctgt gcagggacca
     4621 ggtcgatcga ccggcaccgg cgccatcttc cttgccatag cccgggggga ccttccgttc
     4681 ctcgtcgaca cgactatctc gctcgtggat atcgacgact gtgcccgtgg ccatcttctc
     4741 gccgccgaga agggcctgcg tggtgagcgc tatctcctca gtgggcccgt gctcacgatc
     4801 cgtcaggccg tctcgatgct gcgccgaaag accggacgca ggtcgtcacc tcggtttctc
     4861 cacccgggag tggtgaccgc tgtcgcaccc gtcgtcgggg cggtggccca tctcgtcgga
     4921 gtgcagccgc cgctgtgccc cgagtcggcg cgcgtgctgc ttcatgggca ccaatacgac
     4981 ggctcgcgcg ccacccgaga gctggggctg gagtacacga gcctcgaaga gacgttggac
     5041 cgtaccctgg cctggttcga tgcggagggc ttgctcgatt gaaccatctt cggtgttgat
     5101 cgggcctagc tccgctgact gc
//
LOCUS       DASUTA010000252         2279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1110155, whole genome shotgun sequence.
ACCESSION   DASUTA010000252 DASUTA010000000
VERSION     DASUTA010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110155"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VFZ80_05020"
     CDS             complement(<1..817)
                     /locus_tag="VFZ80_05020"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX6286825.1"
                     /translation="MALVEFVVFHPWRASVILVVGLVLGLGSFYAYQVSTALEAVAVE
                     DFDPAEARAAIADESDVNEQPIPILELDDEPSYDLNAELARIEEQMADVEDDTSSFNV
                     AALGRPIDDSVFDSYLLVGTDASGFLADTIILGLQPTSGSAPIMVSLPRDLFVWNQCR
                     ETFTRLNSGLGGCSSVASGSELLAIMVEDYTGIPVDHLARVNFDGFARVVDAMGGITV
                     CIDNPRRDVKSHLLLEATGCRRVGGEVALAWVRSRHPEELIDGEWRAIGGSDFG"
     gene            complement(848..1249)
                     /locus_tag="VFZ80_05025"
     CDS             complement(848..1249)
                     /locus_tag="VFZ80_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HEX6286826.1"
                     /translation="MSNFDSARVARLLEADKYANELGVELVSVTDGVIVVELEVSDRH
                     TNFLGVGHGGMVFSLADCAFSLASNNAGDAVAIDTHLVLTAASRPGDRLQATVRELSR
                     GRTLGTYRAEVTRSDGRIVGLFTGTVYINQS"
     gene            1265..2200
                     /locus_tag="VFZ80_05030"
     CDS             1265..2200
                     /locus_tag="VFZ80_05030"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007631231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX6286827.1"
                     /translation="MSSAPPFLDFGKTTLRCRGVLALPHIASGKVREIYEIDPDHLLF
                     VATDRISAYDVILNQAIPDKGRVLTGLSLHFFDLLDTPNHLVTTDLSHVGGLTDEERE
                     ELAGRAMVVRRAEVVPVECVVRGYLYGSSWREYRDGGGPTTEHLPDGLQMADKLDEPI
                     FTPATKATTGHDENITEARTREILGDDLYEELRRRSLDIYSRANEYAAEKGVILADTK
                     FEFGHANGELLLIDEVLTPDSSRYWPADQYQPGAEIPSFDKQYVREWLERQPWDKNPP
                     PPDLPDEIVAGTRERYIEAYERITDKSFDDYLAGS"
BASE COUNT          487 a          736 c          695 g          361 t
ORIGIN      
        1 gtccgaaatc ggagccgccg atcgcgcgcc attccccgtc gatgagctcc tcaggatgac
       61 gggagcggac ccaagccagc gccacctcgc caccgacgcg ccgacaaccg gtggcctcca
      121 agaggaggtg tgacttcacg tcccgtcgag ggttgtcgat gcaaacggtg atgccgccca
      181 tggcatcgac cacacgagcg aaaccgtcga agttgacacg cgccagatgg tcgacgggta
      241 ttcccgtgta gtcctcgacc atgatggcga gcagctcgga tcccgatgcc accgacgagc
      301 accctcccag acctgaattg agacgggtga aggtctcccg gcactggttc cagacgaaga
      361 gatcgcgggg caggctgacc atgatcggcg ccgatccgga tgtcggctga agacccaaga
      421 tgatggtgtc ggcgaggaag ccggaggcgt ccgttcccac cagtaagtac gaatcgaaga
      481 cagagtcatc gatgggccgg ccaagcgcgg cgacgttgaa cgagctcgtg tcgtcctcca
      541 cgtcggccat ctgctcttcg atacgggcaa gctcggcgtt cagatcgtaa gacggctcat
      601 catcgagctc gaggatcggg atcggttgct cgttgacatc cgactcatcg gcgatggcgg
      661 ctcgagcctc agcaggatcg aagtcctcga cggccaccgc ctccagcgcg gtgctcacct
      721 gataggcgta gaacgaccca agaccgagga ccagcccgac cacgaggatc acggacgctc
      781 tccaagggtg aaaaacgacg aattcgacca gagccaaaac tcgggaaaat ctaacccaag
      841 gcttcctcta actctggttg atgtagaccg tgcctgtgaa caggcccacg attcgcccgt
      901 ccgaccgggt cacctcggcg cggtatgtgc ccaacgtgcg accccgagac agctcgcgga
      961 ccgtggcctg aagccggtca ccaggacggc tggctgccgt gagcacgaga tgggtgtcga
     1021 ttgccaccgc gtcaccggcg ttgttcgagg cgagcgaaaa agcgcagtcg gccagcgaga
     1081 agaccatgcc cccatgcccg acaccgagga aattggtgtg acggtcgctc acctccagtt
     1141 ccacgacgat cacaccgtcg gtgaccgaga ccagctcgac tccgagctcg ttggcgtatt
     1201 tgtctgcttc gaggagtctc gccacccggg cgctgtcgaa attgctcacg gggacgcagg
     1261 ctagatgtcc tctgcaccgc cgttcctgga cttcgggaag accacgctac gctgccgcgg
     1321 cgtgctggca ctaccccata tcgcatccgg aaaggtcagg gagatctacg agatcgaccc
     1381 ggaccacctc ctcttcgtgg caacggaccg aatctcggcc tacgacgtga tcctgaatca
     1441 ggcaatcccc gacaaaggaa gggtgctcac cgggctcagc ctccacttct tcgatctcct
     1501 cgacacaccg aaccacctcg tgacgacgga cctgtcccat gtcggtgggc tgacagacga
     1561 ggagagagaa gagctcgccg gtcgagccat ggtcgttcgt cgagccgagg tcgtacctgt
     1621 cgagtgcgtc gtccgcggct atctatatgg ctcgtcctgg cgggagtacc gcgacggcgg
     1681 aggacccacc accgagcacc tgccggacgg ccttcagatg gcggacaagc tcgatgagcc
     1741 catcttcact cctgcgacca aggcgaccac cggccacgac gagaacatca cggaggcacg
     1801 gacccgggag atcctcgggg acgacctcta tgaggagctg cgccgtcgtt ctctcgacat
     1861 ctactcgagg gccaacgagt acgccgctga aaagggagtg atcctggcgg acaccaagtt
     1921 cgagttcgga cacgccaacg gcgagctgct gctcatcgac gaggtgctga ccccggactc
     1981 atcgcgttac tggcccgccg accagtacca gcccggtgcc gagatcccgt cattcgacaa
     2041 gcaatacgtc cgagagtggc tggaacgaca gccctgggac aagaacccac cgccgccgga
     2101 tcttcccgat gaaatcgtgg cggggacgcg agaacgctac atcgaggcat acgagcgcat
     2161 caccgacaag agcttcgacg actacctggc ggggtcatga gagtcacggt cacgatcgac
     2221 aggaggcccg agatcgctga ccccgaggga accaccgtga agagggcgct gcacgacct
//
LOCUS       DASUTA010000253         2750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_203117, whole genome shotgun sequence.
ACCESSION   DASUTA010000253 DASUTA010000000
VERSION     DASUTA010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203117"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /locus_tag="VFZ80_05035"
     CDS             <1..123
                     /locus_tag="VFZ80_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX6286828.1"
                     /translation="GGRVRDLPGVRYKVIRGALDASGVSERKQARSRYGSKKVS"
     gene            120..590
                     /gene="rpsG"
                     /locus_tag="VFZ80_05040"
     CDS             120..590
                     /gene="rpsG"
                     /locus_tag="VFZ80_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012297750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX6286829.1"
                     /translation="MMRRAPAQKRSIAPDPVFRSVQVSQVINKVLLKGKKDLARAIVY
                     DALETIEQRTSQDPTVVLKRAVDNIRPSVEVRSRRVGGSSYQVPVEVTPRRQNSLAIR
                     WLVNYARQRREPTMSLRLANEILDASNNTGAAVKRKEDVHKMAESNKAFAHYRW"
     gene            817..>2750
                     /gene="fusA"
                     /locus_tag="VFZ80_05045"
     CDS             817..>2750
                     /gene="fusA"
                     /locus_tag="VFZ80_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX6286830.1"
                     /translation="MSNDNALHHLREVPLSRTRNIGIMAHIDAGKTTLTERILYYTGR
                     NYKIGEVHEGAATMDWMQQEQERGITITSAATTCIWHEHLINIIDTPGHVDFTVEVER
                     ALRVLDGAVAVFDGVAGVEPQSETVWRQADRYHVPRICFINKLDRTGADFYFDVDSIV
                     ERLGATPLVMQLPIGKEADFTGVVDLVEMRAHVWKGDDGKAWETTDIPADLVDQATSY
                     RERLLEQVAETSETLLESYLENGDLTSKEIHEGVRNGTVANDFTPVFCGSAFKNKGVQ
                     LLLDAIVAYLPSPEDLPPTKGFKPGDEEVVLERANNDAEPFAALAFKIMSDPHVGKLT
                     YFRVYSGHLAKGDSVLNTTTGQRERIGRILRMHANHREDVDDVFAGDIVAGIGFKNTT
                     TGDTLAAEQSPIQLESMEFPAPVISVAIEPKTKSDEEKLSSSLHRLAEEDPTFLVRGD
                     EETGQTIISGMGELHLEILVDRLLREFKVDASVGTPQVAYRETIRKGIEKIQYKHVKQ
                     TGGKGQYADVVINLEPTGPGGGYEFVDQIKGGSVPREFIPSVDAGIEEALDQGILAGY
                     PLVDVRAILVDGSSHDVDSSEMAFKIAGSMVLQAAARKAGVKLLEPVMDVEVTTPEEY
                     MGDVIGDLNARRGKVEGMNQR"
BASE COUNT          583 a          870 c          851 g          446 t
ORIGIN      
        1 ggcgggcgag tccgcgacct tccgggtgtc cgctacaagg tcatacgtgg tgctctcgac
       61 gcctccggtg tcagcgagcg caagcaagcc cgcagccgat acggttccaa gaaggtcagc
      121 tgatgcgccg cgctcccgcc cagaagaggt ctatcgcacc cgaccctgtg ttccggagcg
      181 tgcaggtgag ccaagtcatc aacaaggtcc tgctcaaggg taagaaggat ctggcccgtg
      241 ccatcgtcta cgacgcgctg gagacgatcg agcagcggac cagccaggac cccaccgtgg
      301 tcctgaagag ggcggtcgac aacattcgcc cctcggtgga ggtgcgctcc cgccgggtcg
      361 gcggaagctc ctatcaggtt cccgtggaag tcactccacg ccgtcagaac agcctggcca
      421 ttcgctggtt ggtgaactat gcccgtcagc ggcgcgagcc gacgatgtcg cttcgcctgg
      481 cgaacgagat cctcgatgcc tcgaacaaca ccggggcggc tgtcaagcgc aaggaagacg
      541 ttcacaagat ggcggagtcc aacaaggcct tcgcccacta ccgctggtga cgaaccagag
      601 gcagttcgcg tagcgatctg cagcctgctc acgcagtgag caggcacagt tttacaagaa
      661 aatggcgttc cgagaggcgg acctagaagc tcagggcccg aaccacggaa caccgagcgg
      721 cagttcaacg tgaccgaagt cacgcgtgat ctgcaacctg ctcacggaga gcaggcgttt
      781 tcaaacaaat aacgagccgg agtagtagaa gaagaaatga gcaacgacaa cgcgctgcat
      841 cacctacgag aggtgccgct gagccggacc cgcaacatcg ggatcatggc tcatatcgac
      901 gcgggcaaaa ccactctgac cgagcgcatc ctctactaca ccggccgtaa ctacaagatc
      961 ggcgaggtcc acgagggcgc cgccaccatg gactggatgc agcaggagca ggagcgcggc
     1021 atcaccatca cctccgccgc caccacctgc atctggcacg agcatctgat caacatcatc
     1081 gacaccccgg gccacgtcga cttcaccgtc gaggtcgagc gcgccctgcg ggtgctcgac
     1141 ggtgccgtcg ccgtgtttga cggcgtagcc ggagtggagc cccagtcgga gacggtgtgg
     1201 cgtcaggcag atcggtatca cgtacctcga atttgcttca tcaacaagct cgaccgtacc
     1261 ggcgccgact tctacttcga cgtcgactcc atcgtcgagc gtctcggggc gacaccattg
     1321 gtgatgcagt tgccgatcgg caaggaagcc gatttcacag gcgttgtcga cctcgtcgag
     1381 atgagggcgc acgtctggaa gggagacgac ggcaaagcct gggagaccac cgacatcccg
     1441 gccgacctcg tcgaccaggc caccagctat cgcgagcggc tcctcgagca ggtcgccgag
     1501 acctccgaga ccctgctgga gtcatacctc gagaacgggg atctgacttc caaggagatc
     1561 cacgaagggg tccgcaacgg cacggtggcc aacgatttca ccccggtctt ctgcggttcg
     1621 gcgttcaaga acaaaggtgt gcaacttctc ctcgacgcca tcgtcgccta cctccccagc
     1681 cccgaggacc tgccgccgac gaagggtttc aagccgggcg acgaagaggt cgtcctggag
     1741 cgagcgaaca acgacgccga gccgttcgca gccctggcct tcaagatcat gagcgatccc
     1801 cacgtcggga agctcactta tttccgcgtc tactcggggc atctcgccaa gggagactcg
     1861 gtcctcaaca cgaccaccgg tcagcgagag cggatcgggc gcatcctgcg gatgcatgcc
     1921 aaccaccgcg aagacgtcga cgatgtcttt gcgggcgaca tcgtggccgg gatcggattc
     1981 aagaacacga ccacaggtga cacgctggcc gcggagcaga gtcccatcca gctcgagtcg
     2041 atggagttcc ccgcgccggt gatctcggtg gcgatcgagc ccaagaccaa gtcggacgag
     2101 gagaagctct cctcgtcgct ccacaggctc gccgaggagg atcccacctt cctcgtccgt
     2161 ggtgacgaag agacaggtca gacgatcatc tccggcatgg gagagctcca cctcgagatt
     2221 ctggttgacc gtctccttcg cgagttcaag gtcgatgcat cggtgggcac ccctcaggtc
     2281 gcctaccggg agacgatccg caagggcatc gagaagatcc agtacaagca tgtgaagcag
     2341 accggcggca agggccaata cgccgatgtg gtcatcaacc tcgagccgac cggtcccggc
     2401 ggcggctacg agttcgtcga ccagatcaag ggcggctctg tgccacgcga gttcataccg
     2461 tcggtcgatg ccggaatcga ggaggcactg gatcagggaa tcctggccgg ctacccgctg
     2521 gtcgatgttc gcgccatcct cgtcgacgga tcttctcacg acgtcgactc atcggagatg
     2581 gccttcaaga tcgccggctc catggtcctc caggcggccg cgcgcaaggc aggggtgaag
     2641 cttctcgagc cggtgatgga tgtcgaggtg accactcccg aggagtacat gggagatgtc
     2701 atcggtgatc tcaacgcccg ccgggggaag gtcgagggca tgaatcagcg
//
LOCUS       DASUTA010000254         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1757302, whole genome shotgun sequence.
ACCESSION   DASUTA010000254 DASUTA010000000
VERSION     DASUTA010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1757302"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..806)
                     /locus_tag="VFZ80_05050"
     CDS             complement(6..806)
                     /locus_tag="VFZ80_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003952045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX6286831.1"
                     /translation="MAPISDFIDPVAIALDQQVSDWEEAIRSSGHLLEATGVAEPAYT
                     QAMVESVHEHGPYIVVAPGFAFAHARPSEAVLRTGMSWVRLADPIEFGHSSNDPVTLV
                     VALAAKDSSTHQEAMASLARLLGDKETRAALDEAGSPEEVYALLGGSDGTATADGAAQ
                     KPAQPAARAKAGQHRILTVCGNGLGTSLFLKNTLEKVLVGWGWSRFVIVEATDTVSAR
                     GKAKDVDLIFTSGEIARTLGDVGVPMKVIENFTSTIEIDQALRESYDI"
     gene            1063..1320
                     /locus_tag="VFZ80_05055"
     CDS             1063..1320
                     /locus_tag="VFZ80_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX6286832.1"
                     /translation="MPTKSVVVGSAVGLHARPAALISEAAGELDSEVTIAVPGGEPVD
                     ASSALLIMTLGAKKGDTVEVASEDLAALDRIATMVEQDLDA"
     gene            1562..2014
                     /locus_tag="VFZ80_05060"
     CDS             1562..2014
                     /locus_tag="VFZ80_05060"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6286833.1"
                     /translation="MTTSSVTRSMEIDAPPEKVFAFVSDPEKAMEAMDTLGRVVVSDV
                     VTTPDGAVTSRRWSERFRLLPFEIHATVTRQEQFPNQRIVEKHSTGPVCVYTFEPSGN
                     GTRLTYTAELSRPFALLTKMQAFITTKGKGMEHDMDEFLAEIKQQVEA"
     gene            2111..2362
                     /locus_tag="VFZ80_05065"
     CDS             2111..2362
                     /locus_tag="VFZ80_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286834.1"
                     /translation="MLAPLVPRAEAGRRAIDRFAAVELPLRRDADDGSRLGDLLVGHV
                     PRRVRLFGEAPAQPSPLLGRWKLEVAHLHRRTEVGCGLP"
     gene            complement(2276..2626)
                     /locus_tag="VFZ80_05070"
                     /pseudo
     CDS             complement(2276..2626)
                     /locus_tag="VFZ80_05070"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(2639..>2983)
                     /locus_tag="VFZ80_05075"
     CDS             complement(2639..>2983)
                     /locus_tag="VFZ80_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014627052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6286835.1"
                     /translation="EWEQVVVHSVDPVALGQWWAEALGWVVVYSSADEFEIRPEPDRM
                     PGLDFVRLEESKKAKSRLHLDFRPDDQDAEVARLMAHGARRVDIGQGDRPWIVLADPE
                     GNEFCVLGQPHP"
BASE COUNT          529 a          979 c          959 g          516 t
ORIGIN      
        1 ctccctcaga tgtcgtagga ctcgcgaagg gcttggtcga tctcgatcgt gctggtgaag
       61 ttctcgatca ccttcatggg cactccgacg tcaccgaggg tgcgggcgat ctcaccggag
      121 gtgaagatca ggtcgacgtc cttggccttg ccccgggcgg agacagtgtc ggtcgcctcg
      181 acgatgacga agcggctcca gccccagccg acgaggacct tctcgagggt gttcttgagg
      241 aacagactgg tgccgagacc gttgccgcac accgtaagga tcctgtgctg gccggccttc
      301 gctcgcgctg ccggctgagc gggtttctgc gccgctccat cggccgtggc cgtgccatcg
      361 ctgccaccca ggagtgcgta gacctcctcc ggcgagccgg cctcgtcgag ggccgctctc
      421 gtctccttgt cgccgagaag gcgggcgagg ctcgccatcg cctcctggtg tgtcgagctg
      481 tccttggccg cgagcgcgac aaccagcgtc accggatcgt tcgacgagtg cccgaactcg
      541 atggggtccg caagccggac ccaagacatt cccgtccgaa gaacagcctc ggaggggcgt
      601 gcgtgagcga aggcgaagcc gggggccacc acgatgtaag gaccgtgctc gtgcacgctt
      661 tcgaccatcg cctgggtgta ggcgggctcg gcgacccccg tggcctccag gaggtggccc
      721 gatgaacgaa tggcctcttc ccagtccgat acctgctgat ccagcgcgat ggcaacaggg
      781 tcgatgaagt cgcttatggg cgccatagcg ggaccctacg ggatcgcgat ttccattcgt
      841 cagtctggct agaccggact atgtcagcca taaaggccac gtcttgcgag gtcgacatat
      901 gaaatgcatg actggaagaa tcactcgaat caacgagcca aaggttgttt gtcgcggtcg
      961 tcccctcgtg gtcaccctga agcgcttcac gccgagcaac gacgaggtcg aggccgccga
     1021 gtgcgcggtc cggcttctga caagacaacc gaaggagcac acatgcctac caaatccgtc
     1081 gtcgtcggct cggccgtcgg cctccacgcc cgccccgctg cgctgatctc ggaggccgcc
     1141 ggggagctcg acagtgaggt gaccatcgca gtgcccggtg gcgagcccgt ggacgccagc
     1201 tcggctctgc tgatcatgac gctcggcgca aagaagggag acacggtcga ggtcgcaagc
     1261 gaggacctgg cggccctcga ccggatcgcc acgatggtgg agcaggatct cgacgcctga
     1321 gcaagctcga gccgttgaag agagcgctcg ctgggtcccc gcaggtggca gagcagtgcg
     1381 agccctaccg cgcgagacgc tcggtgccaa gccagatgtt ccccgggctt cggcaatcgt
     1441 cgtccacgaa gggagcacgg aagcggcccg gttctcggga ctgctgcggg ccgtttgcct
     1501 ctaggccacg gttgcttcgc cggggacgct ggaggtgcat ggagccatgc gaggaggggc
     1561 catgacgacg tcgtcggtca ccaggagcat ggagatcgat gcccctcccg agaaggtgtt
     1621 cgccttcgtt tccgaccctg agaaggcgat ggaggccatg gacaccttag gtcgagtggt
     1681 ggtcagtgac gtcgtgacca ccccggacgg ggccgtcacc agtcggaggt ggagcgagcg
     1741 attccgtctt ctccccttcg agatccacgc aacggtgact cgtcaggagc agttccccaa
     1801 ccagcgcatc gtcgagaagc actccaccgg tcctgtgtgc gtctacacct tcgaaccgtc
     1861 ggggaacggc acacggctca cctacaccgc cgagctctcc aggccctttg cactcttgac
     1921 caaaatgcag gccttcatca cgacaaaggg caagggcatg gagcatgaca tggatgagtt
     1981 cctggccgag atcaagcaac aggtggaggc ttgacgcgtt tcggacagcg atcggagatg
     2041 ttgagtcggg tgctgcactg ccccgccctg accggaagcc ctgacgccag aggaccgacg
     2101 ggggccagcg atgcttgcgc cactcgtccc acgtgccgaa gccggccggc gggccatcga
     2161 caggttcgct gccgtcgagc tccccttgcg tcgggatgct gatgatggca gccgactcgg
     2221 cgatctcctc gtcggacatg tgccacgtcg tgtgcggctc ttcggcgagg cgccggccca
     2281 gccgtctccg ctgctcggcc ggtggaagct cgaagtagcg catctccacc gccgcaccga
     2341 ggtcggctgc ggcctgccgt agcgcggagc gctcgtctcg accacagaga ccgaagtcga
     2401 cgacgacgtt gatgccgagg gcgagagcgc gcatcccgat ctcgatgagt cgtccttcga
     2461 tcacgtccga ggctgccgac gggttcgcgg acccgtaaag ggccttcacc cactcgtcct
     2521 tcgtgaggcg aagagcctct tcctcgacct ctatccgccg tgcctctgtg gtcttcccgg
     2581 tgcctggcag tcctacggtc aggaacaggg tcggtcgtgt cggcatcact tccgccgact
     2641 agggatgtgg ctggccaagg acgcagaact cgttaccttc ggggtctgcc aagacaatcc
     2701 acggccgatc gccctggccg atgtccacgc gccgcgcgcc atgagccatc agacgagcca
     2761 cctcggcgtc ctggtcatca ggcctgaagt cgagatgcag tcggctcttg gccttcttgc
     2821 tctcctcgag ccggacgaag tccaaccccg gcatccgatc cggctccggg cggatctcga
     2881 actcgtcagc cgaggagtag acgacaaccc aaccaagagc ctcggcccac cactgcccca
     2941 aggccaccgg atctaccgag tgaacaacaa cctgttccca ttc
//
LOCUS       DASUTA010000255         4788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_592117, whole genome shotgun sequence.
ACCESSION   DASUTA010000255 DASUTA010000000
VERSION     DASUTA010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592117"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1795)
                     /locus_tag="VFZ80_05080"
     CDS             complement(<1..1795)
                     /locus_tag="VFZ80_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX6286836.1"
                     /translation="MHVADSHDLIRVTGARENNLKDVDIEIPKRRMTVFTGVSGSGKS
                     SLVFDTIAAESQRLINETYSAFVQGFMPTTARPDVDVLDGLTTAIIVDQERMGANARS
                     TVGTATDANAMFRLLYSRLGEPRIGPPTAFSFNVPTRTASGAMTTDKGKGERKVVRNV
                     VYQGGMCPRCEGMGSVSDIDLTQVYDETKSLNEGAITVPGYKAGGWSVRYFTESGFLD
                     PDKPIAKYSKKELADFLYKDPVKVKINDINLTYEGLVPQIQKSMLSKDVDTLQSHVRE
                     FVERAVTFTPCPDCGGTRLNEAARSSKIAGKSIAEVCAMQISDLAEWVRNLELPTAQP
                     LINALREALDSFVDIGLGYLSLDRPTGTLSGGESQRTKMIRHLGSALTDVTYVFDEPT
                     IGLHPHDVQRMNDLLVQLRDKGNTVLVVEHEPEIIEIADHVVDLGPGAGADGGQVVFE
                     GTVEGLRASGTLTGKHLDDRASVKPEFRTPTDKLEIRGADTHNLQDVDVDVPLGVLVV
                     VTGVAGSGKSSLILGSLPAGEGVITVDQTEIRGSRRSNPATYTGMLDSIRQAFAKAND
                     VKPGLFSPNSDGACPNCNGAGVIYTELGFMDT"
     gene            complement(1842..2255)
                     /locus_tag="VFZ80_05085"
     CDS             complement(1842..2255)
                     /locus_tag="VFZ80_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012296790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6286837.1"
                     /translation="MDITIHSAMLPQTDPEAALAFYRDALGFEVRNDVEYQGMHWITV
                     GPVDQPQTSVVLFPPEASPGLTDDERRVITEMMAKGTYAQINLATHDVDGAFERIEAS
                     EAGEVVQEPTDQPYGVRDCAFRDPSGNMVRIQQLA"
     gene            complement(2266..2640)
                     /locus_tag="VFZ80_05090"
     CDS             complement(2266..2640)
                     /locus_tag="VFZ80_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6286838.1"
                     /translation="MDRDYAQPLNVEVVARVAHMSAGHFSREFKRAYGESPYSYLMTR
                     RVERAMALLRLGEMSVTEVCFAVGFSSLGTFSTRFTELVGVPPSVYRNQSSGDMAGVA
                     PCIAKQVTKPIRNREAQVPVGS"
     gene            2773..3723
                     /locus_tag="VFZ80_05095"
     CDS             2773..3723
                     /locus_tag="VFZ80_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6286839.1"
                     /translation="MSLVFGVQPWNQVFSWAEARHAAIVIEELGEEQLWTWEHALAVM
                     GDPHQDTFDAYTLLAAWSGVTTKVELGVLTGANTFWNPGLLAKKVTTLDHVSDGRAIL
                     GLGAGWFEHEHTAFGIEFGSGFGDRLAWLEESVGALRGLLDGDTVSSPEEGHYSLVEA
                     TLRPRPVREHLPILIGGGGERKTLRTVARYADIWNWVGLRDLDRFRAKHAVFERRCEE
                     EGRDPQEIVRSVFFSPVIRDTEVEALEFFETQMEANNLDDSVLDDPDIYVTTPSDMTE
                     LMIAWKEVGVTSFIIEVAAPFDQETIERIATEIRPDVESA"
     gene            complement(3758..3982)
                     /locus_tag="VFZ80_05100"
     CDS             complement(3758..3982)
                     /locus_tag="VFZ80_05100"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX6286840.1"
                     /translation="MSSITKTIRLSGEAGDSIEDAVRTVLARAATTISDIHKFEIVKV
                     GGEVDDSGAPTVFSVTLDITFGIKDTVEHG"
     gene            complement(4024..>4788)
                     /locus_tag="VFZ80_05105"
     CDS             complement(4024..>4788)
                     /locus_tag="VFZ80_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6286841.1"
                     /translation="SGTSRWWSRVIDHLPTELGIVSLDVRGRGDSHESPPPFDLLTIA
                     DDIARALDHLALDQADLAGYSMGGWIAALFASQHPARAHRVLMVDGGFPIPNPPDVDP
                     DDIIQAVVGPSLARLETDFADEESFFDYWKAHPALENHWDDGMRPALEYELAKANGGH
                     RVRVNPEAIKVNAREITVDPEMRSAGERVSIPAHLIVVERGTADQPGGMIPLQTAERA
                     AREMPNLTMEYLPGVNHYTLVLGAGAPSVAAAIAAW"
BASE COUNT          831 a         1437 c         1564 g          956 t
ORIGIN      
        1 ccgtgtccat gaacccgagc tcggtgtaga tgacgccggc gccgttgcag ttcgggcacg
       61 ccccgtcaga gttggggctg aagagacccg gcttcacgtc gttggctttg gcgaaggcct
      121 gacggatgga gtccagcatc ccggtgtagg tggctgggtt gcttcgccgg gatccccgga
      181 tctcggtttg gtcgaccgtg atcacgcctt cacctgccgg gagagacccc aggatgagcg
      241 agctcttccc cgaaccggca acgcctgtca cgaccacgag gacacccaga ggcacgtcga
      301 cgtcgacgtc ttggaggttg tgagtgtcgg cgcctcgaat ctcgagcttg tcggtgggtg
      361 tccggaactc tggtttcacg ctcgcccggt cgtcgagatg cttgccggtg agggtcccgc
      421 tggctcgtag accctcgacg gtcccttcga agaccacctg accgccgtct gctccagcgc
      481 cggggcccag atccaccacg tggtcggcga tctcgattat ctcaggctcg tgttcgacca
      541 ccaacaccgt gttgcccttg tcgcgaagct ggaccaggag gtcgttcatc cgctggacgt
      601 cgtggggatg aaggccgatc gtgggctcat cgaacacata ggtcacatcc gtgagggcag
      661 acccgagatg gcgaatcatc ttcgtgcgtt gcgactcccc gcctgacagg gtgccggtgg
      721 ggcgatccag ggacagatac ccgagaccga tgtccacgaa ggagtcgagt gcctctctga
      781 gcgcgttgat gaggggctgg gctgtgggta attcgaggtt gcgcacccat tcggcgaggt
      841 cgctgatctg catggcgcag acctcggcga tgctcttgcc cgcgatcttc gaggaccgtg
      901 ccgcctcgtt gagacgggta ccgccgcagt cggggcatgg cgtgaacgtc accgcacgtt
      961 ccacgaactc cctgacgtgc gactgcaggg tgtcgacgtc cttggagagc atcgacttct
     1021 ggatttgggg gacaagaccc tcgtaggtca ggttgatgtc gttgatcttg accttcaccg
     1081 ggtccttgta gaggaagtct gcgagctctt tcttcgaata cttcgcaatc ggtttgtccg
     1141 ggtcgaggaa acccgactcc gtgaagtacc ggaccgacca tccccccgcc ttgtaaccgg
     1201 gaacggtgat ggcaccctcg ttcagcgatt tggtctcgtc gtagacctgc gtgagatcga
     1261 tatcgctcac cgagcccatt ccctcgcagc gtgggcacat gcccccctga tagacgacgt
     1321 tgcgcaccac ttttcgctcg cctttgccct tgtccgtggt catcgccccg ctcgccgttc
     1381 gggtggggac gttgaatgag aatgcggtcg ggggcccgat tctcggttca ccgagccggc
     1441 tgtagagcag ccggaacatg gcgttggcgt cggtggccgt gcctaccgtg gagcgggcgt
     1501 tggcgcccat tcgctcctgg tcgacgatga tggcggtcgt caatccatcg agcacgtcca
     1561 cgtcggggcg ggccgtcgtc ggcatgaagc cctggacgaa ggcgctgtag gtctcgttga
     1621 tcagcctctg agactcggcg gcgatggtgt cgaatacgag agagctcttc ccggagcccg
     1681 agaccccggt gaacacagtc atccggcgtt ttgggatctc gatgtcgacg tcttttaggt
     1741 tgttctcccg ggctccggtg acccgtatga gatcgtggct gtcggcgacg tgcatggcgt
     1801 tcacgctaga tgaggctcgg aaagggtctt ccgccaccgg ctcaggcgag ctgctggatg
     1861 cggaccatgt tgcccgaggg gtcacgaaag gcgcagtcgc ggactccgta tggctgatcc
     1921 gtcggctcct ggactacctc gcctgcctcg ctggcctcga tccgctcgaa tgccccatcc
     1981 acgtcgtgtg tggccaggtt gatctgggcg taggtgccct tggccatcat ctcggtgatg
     2041 acgcggcgct cgtcgtcggt gagacccggt gaggcttcgg gagggaagag caccaccgaa
     2101 gtctggggct ggtcgaccgg gccaaccgtg atccagtgca ttccctggta ttcgacgtcg
     2161 ttgcgcacct cgaacccgag tgcgtctctg tagaaggcca gagcggcctc cgggtcggtc
     2221 tggggcagca ttgccgagtg aatcgtgatg tccataccgg gcaccctaag agccgacggg
     2281 tacttgcgct tctcgattcc tgatcggttt ggtgacctgt ttggcaatgc aaggagcgac
     2341 ccctgccatg tcacccgatg actggtttcg gtacacactc ggtggcacgc cgacgagctc
     2401 ggtgaatcgg gtgctgaagg tgccgagtga ggagaatccc actgcaaaac agacctcggt
     2461 gacgctcatc tcccccaatc gcagcaaggc catggcacgc tcgacccggc gggtcatcag
     2521 atacgagtaa ggagattcgc cgtaggcccg cttgaactcg cggctgaagt ggcccgccga
     2581 catgtgagca acccgtgcca caacctccac attcagaggc tgcgcatagt cgcggtccat
     2641 cgtgtcgcga gcccggcgaa ggaggacgag gtcattgagc tcgttgggtg tcacgcagtc
     2701 agggtactgg tcgcccgatg tgtgttatct gtgatgtcag cagggcgtcg ggtcaagcag
     2761 atacagtgag cggtgtctct cgtcttcggt gtgcagccct ggaatcaggt gttcagctgg
     2821 gccgaagcgc ggcatgccgc aatcgtgatc gaagagctcg gcgaggagca gctgtggacc
     2881 tgggagcacg cgctggcggt gatgggggat ccgcaccagg acacgttcga tgcttacacg
     2941 cttctggccg cctggtccgg ggtgaccacg aaagtcgagc tcggggtgct gacaggtgcc
     3001 aacaccttct ggaatccagg cctgctcgcc aagaaagtca cgacactcga ccatgtgtct
     3061 gacgggcgtg ccatcctcgg cctcggtgcc ggctggttcg agcacgagca caccgcgttc
     3121 ggcatcgagt tcggatcagg gttcggagac cggttggcgt ggctcgaaga gtcggtcggc
     3181 gcactgcggg ggctcctcga tggcgacacg gtctcatcac ccgaggaggg ccactactca
     3241 ctggtcgagg cgacccttcg ccctcggccg gttcgagagc atctgccgat cctgatcggc
     3301 ggcggcgggg agcgaaagac cctccgcacc gtcgctcgat acgcggacat ctggaactgg
     3361 gttggccttc gtgacttgga tcgctttcga gcgaagcatg ccgtcttcga gcgccggtgt
     3421 gaggaggaag gaagggatcc gcaggaaatc gtgcgatcgg tgttcttcag tcccgtgatc
     3481 agggacaccg aggtcgaggc gctcgagttc ttcgagacac agatggaagc gaacaacctc
     3541 gacgactcgg ttctcgatga cccagacatc tacgtcacaa ccccctctga catgacagaa
     3601 ctcatgattg cgtggaagga ggtgggggtc acttcattca tcatcgaggt ggctgcccct
     3661 ttcgaccaag agacgatcga acggattgcc accgagatca ggcccgatgt cgaaagcgcc
     3721 tgagatgcgc ctgagatggt ggcgacgtcg cctccggcta tccgtgctcc actgtgtcct
     3781 tgatgccgaa ggtgatatcg agagtcacgg agaagaccgt cggcgcgccc gagtcgtcga
     3841 cctcgccgcc caccttgacg atctcgaact tgtggatgtc ggagatcgtg gtggcggcgc
     3901 gggccagcac cgtgcgcacc gcatcttcga tcgagtcacc ggcctcgcct gagagccgga
     3961 tggtcttggt tatcgacgac attgaaacaa caagatagtg cccggtaggg cgatgagact
     4021 ctgctaccag gcggcgatgg cagcggccac cgagggggcc ccggccccga gcacgagtgt
     4081 gtagtggttg acgccgggca gatactccat ggtcaggttg ggcatctccc tcgccgccct
     4141 ctccgcggtc tggagcggga tcatgcctcc gggctgatcc gccgttcccc gttccacgac
     4201 gatcaaatga gccgggatgc tcaccctttc tcccgcactc ctcatttctg ggtccaccgt
     4261 gatctcgcgg gcattgacct tgattgcctc ggggttgacc cggacccggt gaccgccgtt
     4321 ggccttggcc aactcatatt cgagtgccgg gcgcatcccg tcatcccagt ggttctccag
     4381 cgcaggatgg gctttccaat agtcgaagaa ggattcttcg tcggcaaagt cggtctcgag
     4441 tctcgccagt gagggcccta ccaccgcctg gatgatgtcg tcggggtcga cgtcgggagg
     4501 attcgggatc gggaaacccc catcgaccat cagcacgcga tgagcccggg ccgggtgttg
     4561 gcttgcgaag agggcagcta tccagccacc catcgaatac ccagccaaat ctgcttggtc
     4621 gagggccagg tgatcgagag cgcgggcgat gtcatcggcg atggtgagca gatcgaaagg
     4681 cgggggtgat tcatggctgt cccctctgcc tctcacgtcc agagaaacga tcccgagctc
     4741 ggtcgggaga tggtcgatga cccgggacca ccatcgcgag gtgccgct
//
LOCUS       DASUTA010000256         3088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_460942, whole genome shotgun sequence.
ACCESSION   DASUTA010000256 DASUTA010000000
VERSION     DASUTA010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460942"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VFZ80_05110"
     CDS             <1..227
                     /locus_tag="VFZ80_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX6286842.1"
                     /translation="RNDRYGNVHVPIGKVSFEIDQLFGNLSALTAEIQRNRPAGAKGR
                     YMRAMTVSSTMGPGVKVDPARLEEIAEKAL"
     gene            complement(603..1406)
                     /locus_tag="VFZ80_05115"
     CDS             complement(603..1406)
                     /locus_tag="VFZ80_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6286843.1"
                     /translation="MDSGYVRHGPCDIYYESHGSGSSILFIHAGVTDSRMWRDQLHMD
                     GYRSVVFDQRGYGKTEWAPGPYSNREDALAVLDHIEIDSAVVVGCSNGGEAAMQLALI
                     APDRVDGLVLVGSAPRGWEPDGGWNDDPLWDEAVKAFEAGDIEAVVELDARMWLAGQG
                     RSLDDIDPNLIELFFDMDRTPAATESERNEYVQTLDPPTNERLEEIAVPALVVVGDRD
                     DPDLVAAAHYLAGQMSERDAVIITGAAHLPSLEKPEAFNAALRSFLSTI"
     gene            1594..2199
                     /gene="rplJ"
                     /locus_tag="VFZ80_05120"
     CDS             1594..2199
                     /gene="rplJ"
                     /locus_tag="VFZ80_05120"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX6286844.1"
                     /translation="MPRPEKVQAVTDIREGIEGARAVFLTEYRGLSVQAVQDLRAALR
                     ESGAEYKVVKMTLAKLAAGDAGIDGLDEYLSGPTALAFANTDPVSTAKALKDFSKDHE
                     VFVLKAGYLSGNILSPEEVSKLAEIEPREVLLGRIAGGAKAPFFKAAGMFAAFNRNAA
                     SVFSQLLDKKESGEFLAGGAEETSIPADEPAETAADKAEEE"
     gene            2203..2601
                     /gene="rplL"
                     /locus_tag="VFZ80_05125"
     CDS             2203..2601
                     /gene="rplL"
                     /locus_tag="VFZ80_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006548361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX6286845.1"
                     /translation="MAKTKMTTDELLATFEEMTVLELKEFLDAFEEKFDVTAAAPVAV
                     AAAPGAGGGDVAAAAADEKDEFDVVITDAGSAKIQVIKEVRGLTSLGLKEAKDLVDGA
                     PAAVLEGVDEETANKAKEALEGAGATVELK"
     gene            complement(2701..>3088)
                     /locus_tag="VFZ80_05130"
     CDS             complement(2701..>3088)
                     /locus_tag="VFZ80_05130"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX6286846.1"
                     /translation="AQLTDHVTAIQGSWVLESFTENGEQTEVEVDVNTAELPVLTFDG
                     YISGTVGCNQIESSFETPYRVVDRTLTFGDVVIEASECGSRLMTAENALMQIALWGAE
                     PIHLTIVDDLMIWEVDRSSFRFGRIP"
BASE COUNT          612 a          966 c          969 g          541 t
ORIGIN      
        1 accgcaatga ccgatacggc aacgtccacg tgcccatcgg gaaggtctca ttcgagatcg
       61 atcagctgtt cggcaatctg agtgctctga cggccgagat ccagaggaac cgcccggccg
      121 gggccaaagg tcggtacatg cgagccatga ccgtgtcttc gaccatgggc cccggagtca
      181 aggtcgaccc ggcccgtctg gaggagatcg cggaaaaggc tctgtagatc ccttctgtgt
      241 cgatttcgta ggcccagaga ccacggacga cacagaacgg ctgcggatta cacttaagaa
      301 aagcccccga acccgggggc ttttctctgg ctgaaactag ggggatctcc gcgcccgtac
      361 atccgtgggc tgagggcatc ggctgccagg tccaccgtcc gcacgccgcc gacgactttc
      421 gtctgtggac cagggtaggg gctggtctat tcgccggctg aacgcggccc tcaaatattc
      481 gggtccgggt agcgggcggg ggcggtcagg gaacccgccc ggcacacgga gaccccctgt
      541 tttcaactaa tcaagtgtcg cgcgtgcctc tcaccctgtc cagggaaaat ctggaaatcc
      601 gctcagatag tgctgagaaa ggaccgaaga gccgcgttga acgcctcagg tttctccaag
      661 gacggaagat gtgcggctcc ggtgatgatc acggcgtctc gctcactcat ctggccggcc
      721 agatagtggg cggcggcaac gaggtcggga tcgtcgcgat caccgacgac caccagggca
      781 gggacggcga tctcctccag ccgctcattg gtcggcggat cgagggtctg gacgtactcg
      841 ttgcgctcac tctcggtggc tgccggggtg cggtccatgt cgaagaagag ctcgatgagg
      901 ttggggtcga tgtcatcgag cgagcgaccc tggccggcca gccacatccg ggcgtcgagc
      961 tcgacgaccg cttcgatgtc ccctgcctcg aaggccttca cagcctcatc ccacaatggg
     1021 tcgtcattcc agccgccgtc gggctcccat cctcgaggcg ccgagccgac gagcaccagc
     1081 ccatccactc ggtccggtgc aattagagcc agctgcatcg cggcctcacc gccgttggag
     1141 catccgacga caacggccga gtcgatctcg atgtggtcca aaacggcgag ggcatcctcc
     1201 cggttggagt acgggccagg agcccactcg gtcttcccat agccgcgctg atcgaagacg
     1261 acgctgcgat agccgtccat gtggagctga tccctccaca tcctcgagtc ggtgactccg
     1321 gcatgaatga agaggatcga cgagccagag ccgtgcgact cgtagtagat gtcacatggg
     1381 ccatgtctca cgtaaccgga gtccatcggg tgagcgtagg ccgaattgcg gagccgccgg
     1441 gaccggatac aattcgcctg caactccaat acctcggccg aagaccgccg gtgtcgaatg
     1501 actcaaagga cgcaccgtcc gccagcgcag gttcgctttt cgaagcccct cgtctttagc
     1561 tgaggggctt tattgatgtc tagggagacg cacatgccaa gaccagaaaa ggtccaagct
     1621 gtgaccgaca tccgagaagg tatcgagggc gcccgtgcgg tgttcctcac cgagtaccgc
     1681 ggactgtccg tgcaggctgt tcaagacctg cgcgccgcct tgcgcgagag cggtgccgaa
     1741 tacaaggtcg tcaagatgac cctggccaag ctggccgcgg gtgacgccgg aatcgacggg
     1801 ctggacgagt atctgtccgg gcccacggcc cttgcgttcg ccaacaccga tccggtgtcg
     1861 actgcaaagg ccctcaagga cttctcgaag gaccacgagg tgttcgtcct caaggcgggc
     1921 tacctatcgg gaaacatcct gagcccagag gaggtctcca agctcgccga gatcgagccg
     1981 cgcgaggtct tgttgggcag gatcgccggc ggtgccaagg cgccgttctt caaggcggcc
     2041 gggatgttcg ctgcgttcaa ccggaacgca gcctcggtgt tctcacagct tttggacaag
     2101 aaagagtcgg gcgagttcct ggccggcggc gccgaagaga cgtcgatccc ggccgacgag
     2161 cccgcagaga ccgcggccga caaggccgag gaggaatgaa gtatggccaa gacgaaaatg
     2221 acgactgacg agctactcgc cacgttcgag gagatgacag tcctcgagct caaggaattc
     2281 ctcgatgcct tcgaggagaa gttcgacgtg actgccgccg cccccgtggc cgtagccgcc
     2341 gcccccggtg ctggtggtgg cgacgtcgcc gctgcagccg ccgatgagaa ggacgagttc
     2401 gacgtcgtga tcaccgacgc cggctccgcc aagatccagg tcatcaaaga ggtccgcggg
     2461 ctcaccagcc ttgggctcaa ggaggccaag gatctcgtcg acggtgcccc cgccgctgtg
     2521 ctcgagggtg tcgacgagga gacggccaac aaggcaaagg aagccctcga gggcgccggc
     2581 gccaccgtcg agctcaagta ggacgcggac ctaccaacca cgcgacctcc ggtagttaga
     2641 cccgtatacg ggcctaacta cctcgagagt gaagtccctg acgaatgtca gggactttct
     2701 ctatgggatc cgaccaaacc ggaacgagga tcggtcgacc tcccagatca tcaggtcgtc
     2761 gacgatggtg agatggatgg gttcggcccc ccaaagcgct atttgcatga gggcgttctc
     2821 ggctgtcatg aggcgactcc cgcactccga cgcttcgatc acgacatcgc caaatgtcag
     2881 ggttcggtcg accacgcgat agggcgtttc gaaactcgac tcgatctgat tgcatcccac
     2941 cgttccgctg atgtagccgt cgaatgtgag cactggcagc tcggcggtgt tcacgtcgac
     3001 ctccacctcg gtctgctcgc cattctccgt gaagctctct aggacccagc tcccctgaat
     3061 cgccgtgacg tggtcggtca attgcgcc
//
LOCUS       DASUTA010000257         4750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1110890, whole genome shotgun sequence.
ACCESSION   DASUTA010000257 DASUTA010000000
VERSION     DASUTA010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110890"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFZ80_05135"
     CDS             <1..492
                     /locus_tag="VFZ80_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6286847.1"
                     /translation="VITDVRLPDRSGIEVCSEITARLPGTKVLILTSYADEDALFSAV
                     AAGAAGFLLKRARASDLVSSIRMVLQGQAVFDHDWAPTEHDPLLGLLSPQERMVASHL
                     ATGMTNRQIAETMGLAEKTVKNYVSSLLNKMGMGRRSEAAAYVARVETSTGRSLVGSE
                     PGR"
     gene            complement(514..1104)
                     /locus_tag="VFZ80_05140"
     CDS             complement(514..1104)
                     /locus_tag="VFZ80_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286848.1"
                     /translation="MTNARSDFLTESEESGQGQDLFRAPGGDQLLEQAREQAEASGWL
                     PAQVADSWPVDDERAMIADRRKRLYDAMRQLESSAARASGQDDWLELVSVATADLRDA
                     LQDHLEETEGDRGFLEDLVRQAPRLGAEARLLREEHRAMVNDCARLTSLIRSHPEDVG
                     AIRKAVLVLLGRLVEHRQRGAELLYDAYNTELGDGD"
     gene            complement(1109..1666)
                     /locus_tag="VFZ80_05145"
     CDS             complement(1109..1666)
                     /locus_tag="VFZ80_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEX6286849.1"
                     /translation="MYDTGMPDPVRVLVSAGSRHGSTKEIGERIGGVLAGYGLDVLVQ
                     DPQHVLDVSDFDVVVLGSAVYAGHWVREAKEVADLIAAESPKPLVWLFSSGPIGEPAK
                     PEEDPVDVTAVLELTEARGHRIFAGKIDKSKLGFGERAILIAVKAPEGDFRDWNEIED
                     WAKEIADHLVPAPAAPKSGESQEKD"
     gene            complement(1801..2193)
                     /locus_tag="VFZ80_05150"
     CDS             complement(1801..2193)
                     /locus_tag="VFZ80_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6286850.1"
                     /translation="MPLALVVADGSWVVNEVSSALAVGSWEIEVLDDPRAIEDRLAES
                     RVDAVIVDMQVGSKGGMAVIRSIRQSTDELSRPRMVLLLDRTADRFLAHRAGADASVL
                     KPINARELRSALGMVTVGAGASHADEEE"
     gene            2300..3505
                     /locus_tag="VFZ80_05155"
     CDS             2300..3505
                     /locus_tag="VFZ80_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX6286851.1"
                     /translation="MPRLVVFGILCVLALSACTEPTGGGIEVIDVSGPLDASALDFIS
                     DSIEEAAAIGQEIAVVQLNSKAVLDGEAFDRLARLVETPPLPVAVWVGPSPAYAFGAA
                     SRLTGLASHSAIAPPDSLLGHFEPAILGEDLGQTDEVVAAADSGLDLQPALRQYLQDL
                     DGATFDTVAGPVTVSTIEEIAGGVTTKPTTLRKAGLDTRFFRLAITPEAAFFFLVIGL
                     TIVVFEFYALGPGVAAGVAALSLILGGWGLLTLPTNWWAFALILLGFVVLITAHQAGG
                     IFALTLVGAVTLQLAGMFLIDGGGQIDPRWWLILPSVLGVLFFFLLAMPTVQRARLST
                     ETIGREGLIGETGVALVDFDPDGLVEVRGARWRGTAHRESGITVGDEIEVTGVDGLYL
                     EVEPRMSQP"
     gene            3763..4041
                     /locus_tag="VFZ80_05160"
     CDS             3763..4041
                     /locus_tag="VFZ80_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007301037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX6286852.1"
                     /translation="MSQASGRSSRLSHPDEWQLDALCRGNHAYLFFPPSTFERKDERE
                     RREMKAKAICGVCPVQGPCSQHALDIREPFGIWGGLTEVERRGMAVAG"
     gene            complement(4067..>4750)
                     /locus_tag="VFZ80_05165"
     CDS             complement(4067..>4750)
                     /locus_tag="VFZ80_05165"
                     /inference="COORDINATES: protein motif:HMM:NF020672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HEX6286853.1"
                     /translation="IDPSASPDHIADALLTIRGRERVQRLSAESRGGSPTAAVVVGDE
                     ALTEKLFASLTARASEMVEDEHAAHPLRPGMSLATLAERLGISPAVAEELVARSSDLI
                     RNGPDVAHKDHQVGLTPTQEEAWARARSRLGGGLSVPVESELGLESDVVAAKVRSGEL
                     VRVSADLVYLPEQIAEIKALLDSMAQDFTVAEFRDRSGLTRKYAVPILEWSDKEGLTV
                     RRGDVRSIR"
BASE COUNT          785 a         1562 c         1488 g          915 t
ORIGIN      
        1 gttatcaccg acgtccgttt gcccgatcgc tccggtatcg aagtgtgcag tgagataacg
       61 gcgcgacttc cgggaaccaa agtcctcatc ctcacctcct atgcagatga agatgccctg
      121 ttctcggcgg ttgcagccgg agcagcgggt ttccttctga aacgggcgag agcatcggac
      181 ctggtgtcga gcatccgcat ggtcttgcag ggacaggccg tcttcgatca cgactgggcc
      241 ccgactgagc acgacccttt gctgggtctg ctctcgccac aagagaggat ggtggccagc
      301 cacctggcga cgggcatgac caaccgccag atcgctgaaa ccatgggcct cgccgagaag
      361 acggtgaaga actacgtgtc cagtcttctc aacaagatgg ggatgggacg gcgtagcgag
      421 gccgccgcat atgtggctcg cgtcgaaacc agcaccggcc gatcgctcgt cggttcggag
      481 cccggacgct aagccaggcg gacgagcccg atatcagtca ccgtcaccga gttcggtgtt
      541 gtacgcgtcg tacaagagct ccgcaccacg ctggcgatgc tccaccagac gaccgagcag
      601 cacgagcacc gcttttcgaa tcgcacccac gtcttcgggg tgagatcgga tcagggacgt
      661 cagacgggcg cagtcgttga ccatggcgcg gtgctcctca cgaagcaacc gagcttcggc
      721 gccaagacgt ggggcctgtc ggaccaggtc ttcgaggaaa cctcgatcgc cctctgtttc
      781 ctcgaggtga tcttgaaggg catcccgaag atcggcagtg gctacggaga ccagctcgag
      841 ccaatcgtcc tggccactcg ccctcgccgc agaagactcg agctgtctca tcgcgtcgta
      901 gagacgtttt ctgcgatcgg cgatcatggc gcgctcgtca tccacgggcc agctgtcggc
      961 tacctgggcg ggaagccagc ccgaggcttc ggcctgttcc cgggcctgct ccaggagctg
     1021 gtcgccgccc ggcgcccgaa agagatcttg gccctggccc gactcctcgg actcggtcag
     1081 gaagtctgat cgtgcatttg tcatcagctc agtccttttc ttgggactca cccgacttgg
     1141 gagcggccgg agccggcacc aggtgatcgg cgatctcttt cgcccagtcc tcgatctcgt
     1201 tccaatcccg gaagtcgcct tcgggcgcct tcactgcgat gaggatcgct cgctctccga
     1261 agcccagctt ggacttgtct atcttcccgg cgaagatcct gtgcccacgg gcctcggtga
     1321 gctcgaggac cgctgtcacg tccaccgggt cctcttcggg tttggctggt tcgccgatcg
     1381 gcccgctcga gaagagccag acgagcggct tcgggctctc ggctgcgatg aggtcggcaa
     1441 cctccttggc ctcgcgcacc caatggcccg cgtagaccgc gctcccgagc acgaccacat
     1501 cgaaatcgct cacgtcgagc acgtgctggg gatcctggac caggacatcg agaccgtagc
     1561 cggccaggac gccgccgatt cgctccccga tctccttggt ggatccgtgc cgtgatcccg
     1621 ctgacaccag aactcgcacg gggtcgggca ttcctgtgtc gtacatgctt gctccgatcg
     1681 tctcgtgtgg tgccagatgg caccctgtca ctacctattc gaaatgacgg ggtgattggc
     1741 gaccagggcc tttggtcact attgcgtgga cctcggggct cgaggggtgc tgggcgccgc
     1801 tcattcttcc tcgtcagcgt ggctggcacc tgccccgacg gtgaccatcc cgagcgctga
     1861 gcgcagctct cttgcgttga tcggtttgag caccgaggca tcggctccgg cgcgatgggc
     1921 cagaaaacga tcggcggtcc ggtcgagcag gagcaccatc ctcggtcgac tcagttcgtc
     1981 ggtggactgg cgtatcgagc ggatcaccgc catgccccct ttcgagccca cctgcatatc
     2041 gacgatgacc gcatcgacac gggactcggc gaggcggtcc tcgatcgcac gtgggtcgtc
     2101 gagcacctcg atctcccaag aaccgactgc gagcgcagag ctgacctcgt tgaccaccca
     2161 cgacccatcc gcgacgacaa gagcaagagg catggccgat gacgttagct ccaactgttc
     2221 tgtgcccgtc tgtggcacca gcgcaacccc aaacccacac agaacggtag gtgtgcgccc
     2281 cgtcctaacc tcccgggtcg tgccacgtct cgttgtcttt ggcattctct gtgttctcgc
     2341 cctgtcggcg tgcaccgagc ccaccggggg tgggatcgag gtgatcgacg tgagcggtcc
     2401 cctggatgcg tcagcgctgg acttcatctc ggactcgatc gaggaagcgg ccgcgatcgg
     2461 ccaggagatc gcagtggtcc agctcaactc caaagcggtc ctcgacggcg aggccttcga
     2521 ccggctggcc cgcctcgtcg agaccccacc gctccccgtc gctgtctggg taggcccctc
     2581 tccggcctac gcattcgggg cagcgtcgcg gctgaccggc ctggccagtc acagcgcgat
     2641 cgccccaccc gactcgctcc tcggacactt cgagccggcg atcctcggcg aggacctggg
     2701 tcagacagat gaggtggtcg cagccgccga ctccgggctc gatctccagc cggcgcttcg
     2761 ccagtaccta caggacctgg atggcgccac cttcgacacg gttgccggcc ctgtgactgt
     2821 gagcacgatt gaagagatag ccgggggtgt gacgaccaaa cccaccacct tgcgcaaagc
     2881 cggcctggat acccggttct ttcggctcgc catcaccccc gaggcagcct tcttcttcct
     2941 ggtgatcggt ctgaccatcg tggtgttcga gttctacgcg ctgggccctg gcgtggccgc
     3001 gggagtcgcc gcgttgtctc tgatcctggg agggtggggt ctgctgactc tgcccaccaa
     3061 ttggtgggca tttgccctga tccttctcgg cttcgtggtg ctcatcacgg ctcaccaagc
     3121 aggggggatc ttcgcactca cgttggtagg cgcggtgact ctccaactcg ccgggatgtt
     3181 cctgatcgac ggtggcggcc agatcgaccc caggtggtgg ctgatacttc cttcggtcct
     3241 cggggtgctc ttcttcttcc ttctcgccat gcccacggtg cagcgggccc gcctctccac
     3301 cgagaccatc ggccgggaag gcctcatcgg cgagaccgga gtggctctcg tcgacttcga
     3361 ccccgacggt ctcgtcgagg tgcgcggggc gcggtggcgg ggaaccgctc atcgtgagtc
     3421 ggggatcaca gtcggagacg agatcgaggt aaccggcgtg gacggtctct atctcgaggt
     3481 ggagccccgg atgtctcaac cgtgacgact cgacaactcg cggtcgagcc caccgccgcc
     3541 cccgctcgtc tttccgggcg ttctcggggc ctgacacttc gctgagcgga gtctgcaacc
     3601 ctgggtttga gccacccgct cgccaatcgc gttctctggg cccgacactt cgcagagcga
     3661 agtcgccaac cctgggtttg agcacagtcc gcgaaacata agcggcccct atgccggatt
     3721 cggtcttgaa cctttgttga acattcgtta gcttcgtcca ggttgtcgca agcaagcggg
     3781 aggagttcga ggttgtccca ccctgacgaa tggcagctag atgcgctttg tcgtgggaac
     3841 cacgcctacc ttttctttcc cccatcgacc ttcgagagga aggacgagag ggagcgacgt
     3901 gagatgaagg caaaagccat atgcggtgtg tgcccggtcc aagggccgtg ctcacagcat
     3961 gctctggaca ttcgtgagcc gttcgggatt tggggcgggc tcaccgaggt tgaacggaga
     4021 ggcatggccg tagcaggctg aaggttctca cttgagcgac acgggctcac ctgatgctgc
     4081 ggacatcacc gcggcggacg gtgagccctt ctttgtcact ccactcgagg atcgggactg
     4141 cgtacttgcg ggtgagccct gatcggtcgc ggaactcggc cacggtgaag tcttgcgcca
     4201 tggagtcgag gagggccttg atctccgcga tctgctccgg gaggtagacc agatcggccg
     4261 agacacgcac cagctctccc gatctcacct ttgcggccac cacatcggat tcgagcccga
     4321 gctcggattc gacgggcacg ctcagaccgc ccccgagacg cgatcgggcc ctggcccagg
     4381 cttcttcttg agtgggtgtg aggccgacct ggtggtcctt gtgtgccaca tccgggccgt
     4441 tgcggatgag gtcgctcgat ctcgccacca gttcctcggc caccgccggc gagatgccca
     4501 gtcgctcggc caaggtggcg agggacatgc ctggcctgag cgggtgagcg gcgtgctcgt
     4561 cctccaccat ctccgaggcg cgcgccgtca gacttgcgaa gagcttctcg gtcaatgcct
     4621 cgtctccgac aaccaccgcc gcggtcggcg atccgccccg gctctcagcc gacagccgct
     4681 gtacacgctc ccgacctcga atggtgagaa gcgcgtcggc tatgtgatcg gggctcgccg
     4741 acgggtcgat
//
LOCUS       DASUTA010000258         3477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_592746, whole genome shotgun sequence.
ACCESSION   DASUTA010000258 DASUTA010000000
VERSION     DASUTA010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592746"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2499
                     /gene="polA"
                     /locus_tag="VFZ80_05170"
     CDS             <1..2499
                     /gene="polA"
                     /locus_tag="VFZ80_05170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX6286854.1"
                     /translation="FTRMLIRLIKDHHPEGVAVAWDVSRQTFRTESYPEYKANRDKAP
                     DHFRSQLPLIDEVLESLHITQLRREGYEADDIIATLTKRAVADGWEVLIVTGDRDAFQ
                     LVGGPVRVLYTRRGISDTVLADEDWVTEKYGISPSQYVEYAALRGDTSDNLPGVPGVG
                     EKTAAKLIAEYGSIDGVFTAVADQTPKLRENLSAHRDQVFLNRELMALVDDLTVGTGT
                     SDFATREWDKRTVKDLFDSLEFHTMWNDLEEALPSTGGATEVLEIESELVSTADRVGA
                     LADRERLVLGLVNDGGEPYGIAVSTGEGTAAIIPMDAAEPILERISSGDIAVAGHDLK
                     SLARALLELGHDLTNPEMDTALASYIINPSQRTYELEEIAERVLGLELVSPDDEDSEG
                     MLPFDEGPDVDLEGRRVEAVRQLVETLRDELEMREELDLFTTYELPLVPVLARMEHAG
                     IGVDRAYLEEMGADLREKIGTLEKKCHELAGEPFNVNSTDQLRAILFDQLQLPVLKKT
                     STGKPSTDASVLKKLNHPLVDALLEYRELEKLRSTYVDGYLPLVDPDGRIRTRFNQMA
                     ATTGRLSSDSPNLQNIPIRSESGRTIRRAFIAGRGNEFLVADYSQIELRVLAHMSGDP
                     FLIEAFQAGLDIHTATASRVWGVPTDEVTANQRRTAKMINFGLLYGMEAFGLADRLGI
                     GREEAKEHIDAYFSQFTDVKEYMQSVVTLARNQGYTTTLFGRRRYLPELKSDNFRIRQ
                     MGERMALNAPVQGTAADIIKAAMIELDATLREEKLATTLLLQIHDELILEVPEEERVV
                     AEKLVIETMEGVTGLDVPLKVDVAWGQDLASVKM"
     gene            2716..>3477
                     /locus_tag="VFZ80_05175"
     CDS             2716..>3477
                     /locus_tag="VFZ80_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX6286855.1"
                     /translation="MSNPQENASVAAPENEETVETPETPAAEENAEEAASTPESETAP
                     EAADSSAPETTEPAAAAPEATTEEASSDESDGEAAQGRYVEREALVAPDLSDLPDDIG
                     DDFERAIEATVLEFREGDIVEGTIVNVDSEGALVDIGYKSEGFIPTRELSIRNNIDPE
                     ETVEVGDRVESVVLDKEDDEGRLILSKKRAMYERAWGQIQKIADEDGTVEGTVIEVVK
                     GGLIVDIGLRGFLPASLVDLRRVRDLDPFIGESITA"
BASE COUNT          690 a         1149 c         1098 g          540 t
ORIGIN      
        1 ttcacccgga tgctgatccg gctcatcaaa gaccatcacc ccgagggtgt cgccgtcgcc
       61 tgggatgtct cccgccagac cttccgcacc gagtcgtatc ccgagtacaa ggcgaatcgg
      121 gacaaggcgc cggaccactt ccgcagtcag cttccgctga tcgacgaggt cctcgagtcg
      181 ctccacatca cccagctgcg tcgcgagggc tacgaggccg acgacatcat cgccacactc
      241 accaagcgag cggtagccga cggatgggag gttctcatcg tcaccggcga ccgcgatgca
      301 ttccaattgg tcggtgggcc cgtgcgggtc ctctacaccc gacggggcat cagtgacacc
      361 gtgttggccg acgaggactg ggtgaccgag aagtacggca tctccccgtc ccagtacgtg
      421 gaatacgccg cgctccgggg cgacacctcc gacaacctcc ccggtgtgcc cggggtaggg
      481 gagaagaccg cggccaagct catcgccgag tacggctcta tcgacggggt cttcaccgcc
      541 gtcgccgacc agaccccgaa gctgagggag aacctgtcgg cgcaccgcga ccaggtcttc
      601 ctcaatcggg agctgatggc actcgtcgac gacctgaccg tgggcacggg caccagtgac
      661 ttcgcgacca gggagtggga caagcggacc gtcaaggacc tcttcgacag tctcgagttc
      721 cacacgatgt ggaacgacct cgaagaggcc ctgccctcga ccgggggcgc aaccgaggtg
      781 ttggagatcg agtccgagct ggtgagcacc gccgaccggg tcggcgccct ggcagatcgg
      841 gagcgtctcg tgctcggcct ggtcaacgat gggggagagc cctatggcat cgccgtctcc
      901 accggcgaag gcacggcggc gatcatcccg atggacgccg ccgagccgat cctggagcgc
      961 atcagctccg gagacatcgc ggtggccgga cacgacctga agagcctggc tcgcgccctc
     1021 ctcgagttgg gccatgacct caccaatccg gagatggaca ccgccctcgc ctcctacatc
     1081 atcaatccga gccagcgcac ctacgagctc gaggagatcg ccgagcgggt actcggcctc
     1141 gagctggtct cacccgacga cgaggacagc gaggggatgc tccccttcga cgagggccct
     1201 gacgtcgatc tcgagggccg acgggtcgag gcggtgcgtc agctggtcga gaccctgcgt
     1261 gacgagttgg agatgcgtga ggagctcgac ctgttcacca cctacgagct cccgctggtg
     1321 cccgtcctgg ctcgaatgga gcacgccggc atcggtgtgg acagggccta tctggaggag
     1381 atgggagccg acctccggga gaagatcggc accctcgaaa agaagtgtca cgagctggcg
     1441 ggcgagccat tcaacgtcaa ctcgaccgac cagctgcgcg ccatcctctt cgaccagctc
     1501 caactgccgg tgctgaagaa gacatccacc gggaagccct ccaccgacgc ctctgtgctg
     1561 aagaagctga accacccgct ggtcgacgcc ctcctcgagt accgggagct ggagaagctc
     1621 cgttccactt acgtggatgg ctacctgccc ctggtcgacc cagacgggcg cattcgcacc
     1681 cggttcaatc agatggccgc caccacgggg cggctctcct ccgacagccc caatctgcag
     1741 aacatcccga ttcggtccga gtcggggcgg accatccgcc gcgccttcat cgccggcagg
     1801 ggaaacgagt tcctggtcgc cgactactcc caaatcgagc tgcgcgtgct tgcccacatg
     1861 agcggcgatc cgttcctcat cgaggcgttt caggccgggc tcgacatcca caccgcaacc
     1921 gcgtcgcgag tctggggcgt gcccaccgac gaggtgaccg ccaaccaacg ccgcaccgcc
     1981 aagatgatca acttcggcct tctctatggc atggaggcgt ttgggctcgc cgaccggctc
     2041 gggatcggcc gcgaggaggc caaagagcac atcgacgcct acttctctca gttcacagac
     2101 gtaaaggagt acatgcagtc ggtggtcacg ctggcccgga accaggggta cacgacgacg
     2161 ttgttcggcc ggcgccgata tctgcccgag ctgaagtcgg acaacttccg catcaggcag
     2221 atgggggaga ggatggcgct caatgcaccc gtgcagggaa ctgcggccga catcatcaag
     2281 gcggcgatga tcgagctcga cgccacgctg cgggaagaga agctggcgac gacgctgctc
     2341 cttcagatcc acgacgagct catcctcgag gtgcccgagg aggagagggt ggtggccgag
     2401 aagctggtga tcgagacgat ggagggggtc accggcctcg atgtccccct caaggtcgat
     2461 gtcgcctggg gccaggacct ggcgtcggtc aagatgtgac atgcgtgtgc agataccgtg
     2521 gcctggccac cgtatctgca cacgcttgag ccgctcaccc cccaatcccc cggttcccag
     2581 cgtcgttcac cgacgctggg aacacatggt tcgggtacta aggggttggc gaaaacctgc
     2641 cgtgtgcgta cacttcgatt gcctgccggc cgttcgcgcc cggcagagat cttcccaaga
     2701 ggtaactacc cacacatgtc caacccccaa gaaaacgcgt ctgtagccgc tccagaaaac
     2761 gaggagacgg tggagacgcc cgagaccccg gcagccgaag agaacgcaga agaggcagcc
     2821 agcacccccg agtccgagac ggcacccgaa gccgccgact catccgctcc cgaaaccaca
     2881 gagcccgcag ccgcggcacc tgaggcgaca accgaggaag catcctccga cgaatccgac
     2941 ggggaggccg cccagggcag gtacgtcgag cgtgaggctt tggtcgctcc cgatctgagc
     3001 gaccttcccg acgacatcgg cgacgacttc gagagggcga tcgaggcaac cgttctcgag
     3061 ttccgtgagg gcgacatcgt ggaagggact atcgtcaacg tcgattccga gggagccctc
     3121 gtcgacatcg gctacaagtc agagggcttc atccccaccc gcgagctctc cattcgcaac
     3181 aacatcgacc cggaagagac cgtcgaggtc ggcgaccgcg tcgagtcggt cgtgctggac
     3241 aaggaagacg atgaaggtcg tctcatcctc tccaagaagc gcgccatgta cgagcgtgcc
     3301 tggggccaga tccagaagat cgccgacgaa gacggaaccg tcgaaggcac ggtcatcgaa
     3361 gtggtcaagg gcggtctcat cgtcgatatc gggttgaggg gattcctccc tgcttccctg
     3421 gtggatctgc ggcgggtgcg cgatctcgac ccgttcatcg gcgagagcat cacggcc
//
LOCUS       DASUTA010000259         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1370853, whole genome shotgun sequence.
ACCESSION   DASUTA010000259 DASUTA010000000
VERSION     DASUTA010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370853"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFZ80_05180"
     CDS             complement(<1..323)
                     /locus_tag="VFZ80_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018567895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEX6286856.1"
                     /translation="MESSRSEGNRSEGNRLTPVKIVVAGGFGVGKTTFVGAISEIEPL
                     RSEAIITEASEGIDELDLGTSKTMTTVAMDFGRISLKHDLVLYLFGTPGQDRFHFMWN
                     ELARGA"
     gene            complement(304..681)
                     /locus_tag="VFZ80_05185"
     CDS             complement(304..681)
                     /locus_tag="VFZ80_05185"
                     /inference="COORDINATES: protein motif:HMM:NF017170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
                     /protein_id="HEX6286857.1"
                     /translation="MSVWDPPHIQVGGRIVRPYTMTGGRTEAGERAIALEALVVATAE
                     GVRNRHRYRWESAEIITLSRKETAVIELAALLDVPIGVVKVLTGDLLEQGAVSITDPP
                     ATDTEGEGYTDLLNKVLDGIKSL"
     gene            complement(678..1103)
                     /locus_tag="VFZ80_05190"
     CDS             complement(678..1103)
                     /locus_tag="VFZ80_05190"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX6286858.1"
                     /translation="MSVTEVASNLNWLLGRLTENVPGIKQAVVVSSDGLALAKSDGVD
                     RETAERLAAVSSGMIGLAYGSAGRFGAGPVSNVIVEMQRGWLFVTGIRDGSLICCLTE
                     KEIDMGAIAFEMSIFVQRVGESLTPDVRQELKALLVEPE"
     gene            complement(1090..>2215)
                     /locus_tag="VFZ80_05195"
     CDS             complement(1090..>2215)
                     /locus_tag="VFZ80_05195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6286859.1"
                     /translation="AQAFNSMQATLVDVAHQQVDVLRRGVSDIFVTMARRNRSLIDRQ
                     LAMLDEFEAEVEDPEVLAHYYQLDHMATRMRRNSESLLVLANAEPKRRRVKATEIDDV
                     VRASIGEVEDYRRIEIEALESLQVRGNVVADISHLLAELLENAVQFSPPSEPVRVTGL
                     FESDGYQVTVSDRGVGMTEARVAELNRILEKPPVLGLSVEPTLGMYVVSKLAHRHGVK
                     VELIRGVPGITARVTVPRNHLEAVDKAQIGNLERDRRRARSAPDPDDYDDAATRDYIL
                     KHTATEAEPAPVASAEPDRVIDLTREHVEDELTPASPSAGGLPIRNPGQSLGDDDSSA
                     PATGPGEGAVGIKSALSAFDAGRRAAEVKNDDEDDDRERH"
BASE COUNT          366 a          725 c          710 g          414 t
ORIGIN      
        1 gccccgcggg cgagctcgtt ccacatgaag tggaacctgt cctgtcccgg tgtgccgaac
       61 agatagagga cgaggtcgtg cttgaggctg attcggccga agtccatggc caccgtcgtc
      121 atcgtcttgg aggtcccgag gtcgagctcg tcgattccct cgctggcctc ggtgatgatc
      181 gcttcgctgc gcagaggctc tatttcggag atggctccca cgaaggtggt cttgcccaca
      241 ccgaaaccac ctgcgacgac gatcttgacc ggtgtcagac ggttcccctc ggagcggttc
      301 ccctcagagc gacttgattc catcgagcac cttgttcaac aggtcggtgt agccctcacc
      361 ctcggtgtca gtggcgggtg ggtccgtgat gctgaccgct ccttgttcga gcaggtcacc
      421 ggtgagcacc ttcacaacgc cgatcggcac gtcgagcagg gcagccagct cgatgactgc
      481 cgtctccttg cgactgagcg tgatgatctc agccgactcc catcgatacc ggtgcctgtt
      541 gcgtaccccc tctgcggtcg ccaccaccag ggcttcgagc gcgatcgctc tctcgccggc
      601 ttcggtccgt cctccggtca tcgtgtacgg tcgcacgatg cgacccccga cctggatgtg
      661 gggtgggtcc cagacgctca ttcaggctct accagcagag ccttgagctc ctgacgcacg
      721 tcaggcgtga gactctcgcc aaccctctgc acgaagatcg acatctcgaa ggcgatggcg
      781 cccatgtcga tctccttctc ggtgagacag cagatcagcg agccgtcgcg gatcccggtg
      841 acgaacagcc acccgcgttg catctcgacg atgacattgg aaacgggacc ggcgccgaac
      901 cgcccggctg atccgtaggc gaggccgatc atgcctgagg agactgcggc caggcgctcg
      961 gccgtctccc gatcgactcc gtccgacttg gccagcgcaa gaccgtccga tgacacgacc
     1021 acggcctgtt tgatgccagg aacgttctcg gtgagccggc ccaggagcca attcaggttg
     1081 ctggcaacct cagtgacgct cacggtcgtc atcctcatcg tcattcttca cctcggcggc
     1141 acgacgacct gcgtcgaatg cggagagggc cgacttgatg cccaccgcgc cctcacccgg
     1201 gccggtggcc ggagcagagg agtcgtcatc gccgagagac tggccaggat tgcgaattgg
     1261 gagcccgccc gccgatggag aagctggggt cagttcatcc tcgacgtgct ccctggtgag
     1321 atcgatcacc cgatcaggtt cggcggatgc gacgggagcc ggctcggctt cggtggcggt
     1381 gtgcttgagg atgtagtccc gggtggcggc atcgtcgtag tcgtccgggt ccggtgccga
     1441 ccgagccctc cgccgatcgc gctccaggtt tccgatctgc gccttgtcca ccgcttccag
     1501 gtggttccgg ggcaccgtca cacgggctgt gatgccggga accccgcgga tcaactcgac
     1561 tttgactccg tgacgatgcg cgagtttcga caccacatac atcccaagtg tcggctccac
     1621 ggacaggccc aacactggtg gcttctcgag gatgcggttg agctcggcga cacgggcctc
     1681 ggtcatgccg actccccggt cggagacggt gacctggtac ccatccgatt cgaacagacc
     1741 ggtcacccga accggctcgg aaggcggaga gaactgaacg gcgttctcca gcagctcggc
     1801 gaggaggtgg gagatgtcgg cgacgacgtt cccacgcacc tgcagcgact cgagcgcctc
     1861 gatctcgatc cggcggtagt cctcgacctc accgatggag gcgcgaacga cgtcgtcgat
     1921 ctcggtcgcc ttcacccgcc ggcgcttggg ctcggcgttg gcgagcacca gcagcgactc
     1981 gctgttgcgg cgcatgcggg tggccatgtg gtcgagttgg tagtagtggg cgagcacctc
     2041 ggggtcctcg acctctgcct cgaactcgtc gagcatcgcc agctggcggt cgatcagcga
     2101 ccggttgcgg cgggccatcg tgacgaagat gtcggacacg ccacggcgca ggacgtcgac
     2161 ctgttgatgg gcgacgtcga cgagcgtggc ctgcatcgag ttgaacgctt gcgcc
//
LOCUS       DASUTA010000260         2501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1370858, whole genome shotgun sequence.
ACCESSION   DASUTA010000260 DASUTA010000000
VERSION     DASUTA010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370858"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFZ80_05200"
     CDS             complement(<1..346)
                     /locus_tag="VFZ80_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX6286860.1"
                     /translation="MEIPMYGILDSPEPAESNERADNQLESLRTEVDRIDDGILGLLS
                     ERRQVARQIAAEKRKTERPFRDDTREEQLLIERLASAGRYDLEPDLVSRVWEQIMADS
                     LRVQFDYVQSTVN"
     gene            552..1571
                     /locus_tag="VFZ80_05205"
     CDS             552..1571
                     /locus_tag="VFZ80_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX6286861.1"
                     /translation="MAMRKATILGTGLLGASVGLALRSQGWSVSGWDPDPDALDQATG
                     RGAIEKRLAGPSLADTDLIVLAAPPEAVIESLQSMDTDSLVTDIAGVKVPVITAADHL
                     PHFVGGHPMAGGETSGPALAASSLFQGATWVLTSDGATEHDLESVGEIVSSFGANPVV
                     MTAQEHDAAVARVSHLPHLLAAALMSLAQSDTATLGLAGGGFRDLTRIASGDSKWWAE
                     VLTANSSQVGASIDELQRALATWKDALTSGGSLAAKLDSARTARQSLGEHNTPVRVVL
                     LDRPGEIARVGHALEKSRVDVRDFQLRHGEHGGGGILTISVRAEGKDALTAALIDEGF
                     EVRVD"
     gene            complement(1572..>2501)
                     /locus_tag="VFZ80_05210"
     CDS             complement(1572..>2501)
                     /locus_tag="VFZ80_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6286862.1"
                     /translation="AITSVLALENGEIEAYRGNYSYYLEERELRRERKERERKHQDEA
                     IARLEENIRRFKGTTEKMAKTARAWETRVERMKRELVEVQKGGKKVRLAFPQPQRSGR
                     VPLAATGLAKSFGDNLVFLDIEFAVERGERMLIIGLNGAGKTTLLRILAGVETADLGE
                     VSLGHQASLGYYAQEHEQIFDGVTVFDHMRRVAGLPDATLRSLLGHFLLADKVDQDAS
                     TLSGGEKTKLALAMLVAERPNVLLLDEPTNNLDPQAKEALLDALTMYDGTIVMVSHDT
                     DFVAQMMPDRAVLMPDGEMKFFDEELLDLVALA"
BASE COUNT          433 a          800 c          810 g          458 t
ORIGIN      
        1 cgttgaccgt gctctgcacg tagtcgaact ggacacgcag ggaatcggcc atgatctgct
       61 cccagacccg tgacaccagg tccggctcga ggtcgtaacg acccgcagaa gccagtcgct
      121 cgatcaagag ctgctcctcg cgggtgtcgt cgcgaaacgg acgttcggtc ttgcgcttct
      181 cggcggcgat ctgtctggcg acctgtctcc tctcggagag aaggccgagg atcccgtcgt
      241 cgattcgatc gacctcggtt ctcagggatt cgagttggtt atcagctcgt tcgttcgatt
      301 ctgcgggctc tggggagtcg agaatgccgt acatcggtat ctccaatgga agatgacgct
      361 cctgggagcg gtcgggcaat aagggaaagg cgggggtggg gtagtgcggc gctaggcgcc
      421 gaaatagccg gcaaagtacg cgcggaagga cagggagtcc cgaacgaatg cgcgattgcg
      481 gctcatcgag tctgaattgt taccgcgttc gtcgattacg tcaagaggac gaaccctaag
      541 gtcgcccctt catggccatg cgaaaagcga cgatcttggg taccgggctc ctcggagcct
      601 cggtcgggct cgcactgaga tcccagggat ggagcgtttc cggatgggac cctgatcccg
      661 acgccctgga ccaggcaacg gggcggggcg ccatcgagaa acgactagct ggtcccagcc
      721 tcgccgacac cgacctgatc gtcctcgctg cgccccctga ggctgtcatc gagtccctgc
      781 agtcaatgga cacagactcg ctggtcaccg atatcgcagg tgtcaaggtg ccggtgatca
      841 ctgctgcaga tcaccttccc cacttcgtcg gcggtcatcc catggccggc ggggagacca
      901 gtgggccggc actcgccgcc tcgtcgctgt tccagggagc cacctgggtc ctgacgtcgg
      961 acggcgccac cgaacatgac ctggaatcgg tcggcgagat cgtcagctcg ttcggcgcca
     1021 atcctgttgt catgacggcg caggagcacg acgccgccgt ggctcgtgtc agtcacctcc
     1081 cccacctcct ggccgccgct ctcatgagcc ttgcccagtc ggacaccgcc accttggggc
     1141 tggccggagg tgggtttcgc gacctgacca ggatcgcctc cggcgacagc aagtggtggg
     1201 cagaggtcct gacggcaaac tcgagccaag ttggagcctc gatcgacgag cttcagcggg
     1261 cgttggcgac gtggaaggat gcattgacca gtggcggcag tctcgccgcg aagctcgact
     1321 cggcccgcac cgcgaggcag tcgctgggcg agcacaacac gccggtgcgc gtcgtgctcc
     1381 tcgaccggcc gggtgagatc gcacgcgtgg gccacgccct cgagaagagt cgggtcgacg
     1441 tcagggactt ccaactgcga catggcgagc acggcggagg cgggatcctc accatctcgg
     1501 tgcgagccga aggcaaggac gcgttgaccg cggccctgat cgacgagggc ttcgaggtgc
     1561 gggtcgacta gctaagccag agccaccaga tcgagaagct cctcgtcgaa gaacttcatc
     1621 tcaccatcgg gcatcaagac cgctctgtcc ggcatcatct gggccacgaa atcggtgtcg
     1681 tggctgacca tcacgatggt cccgtcgtac atcgtcaacg catcgaggag ggcctccttg
     1741 gcctgggggt ccaagttgtt cgtgggctcg tcgagcagca gcacgttggg gcgctcggcg
     1801 accagcatgg ccagagcgag cttggtcttc tccccgccgg agagggtgct cgcgtcctga
     1861 tcgaccttgt ctgcgagcag gaagtgaccg agcagggagc gaagcgttgc atcagggaga
     1921 ccggcgacgc ggcgcatgtg gtcgaacacc gtgaccccat cgaatatctg ctcatgctcc
     1981 tgcgcgtagt agccgaggct cgcctggtgc cccagggaga cctcgccgag gtctgccgtc
     2041 tccacgccgg cgaggatcct gagcaacgtc gtcttcccgg caccgttcaa accgatgatg
     2101 agcatccgct ctccccgctc gacagcgaac tcgatgtcga gaaagacgag attgtcgccg
     2161 aacgacttgg cgagacccgt ggccgccagc gggacccggc ccgaccgctg tggctgcggg
     2221 aaagcgagac ggaccttctt gccacccttc tggacctcga cgagctccct cttcatccgc
     2281 tcgacccggg tctcccatgc tctggcggtc ttggccatct tctcggtggt gcctttgaat
     2341 cggcggatgt tctcctcgag ccgggcgatg gcctcgtctt ggtgcttccg ctcccgctcc
     2401 ttgcgctcgc gccgcagctc tcgctcctcc aggtagtagg agtagttgcc gcggtacgcc
     2461 tcgatctcgc cattctccag agcgagcacc gatgtgatgg c
//
LOCUS       DASUTA010000261         2300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1889110, whole genome shotgun sequence.
ACCESSION   DASUTA010000261 DASUTA010000000
VERSION     DASUTA010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889110"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VFZ80_05215"
     CDS             complement(<1..216)
                     /locus_tag="VFZ80_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEX6286863.1"
                     /translation="MYRKREIRGHQNDFDGYVKRIAFHFDRLEGAERSSHQEYVDAQN
                     STWLPIARGLGNVLWLILAGWWLAAVHF"
     gene            complement(268..591)
                     /locus_tag="VFZ80_05220"
     CDS             complement(268..591)
                     /locus_tag="VFZ80_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX6286864.1"
                     /translation="MVARNDIYWADLGEEGGRRPVCVLTRNSVISVLDRVTCAPITRT
                     VRGIPSEVEVGPAQGLPDDAVINCDNIITIGKQLLDPRPVGSLDEITRAKLDQALRFS
                     LDILY"
     gene            complement(585..830)
                     /locus_tag="VFZ80_05225"
     CDS             complement(585..830)
                     /locus_tag="VFZ80_05225"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEX6286865.1"
                     /translation="MPRREVLVQLDDELVERLDEIAQRTQVSRSELLRRAAQSVIEAD
                     EWAQADKRLVEGYTKYPPDPLLLQASERLAAETAPEW"
     gene            1090..>2300
                     /locus_tag="VFZ80_05230"
     CDS             1090..>2300
                     /locus_tag="VFZ80_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015301924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286866.1"
                     /translation="MARSMAFLLFVSILVLPLPASALDVTQSEHVQLGFTAELPISET
                     ISESCCLDTGEYTVEFDGEATLMVDMGADLEVTYDLADLVDGGSMPIALEYTPTNDAG
                     PELTLDVSGDFGLDFCLLLIICDGDTVSDVTFVAGAGDFTAPLAGDAPVLIPLSSDTL
                     EFFVGPFKVADAHIEGDLSLAPVPSGSFPGLGGAAAGLAVGGSGTLTDPAPIPMVEWQ
                     TAGSQPGTVLLDDPLGGEVDLTASPVVHWLAPSLDVDLVVDLVDEINFFGFDDVTESL
                     ISGDLGSELQAAGLDTLVADAIGGLAGAAVGSRIAAGFIPIPLLDPALDTISLGSTPT
                     IGSVEFVIDTDSDDDGLTDGEEIAIGTDPFDPDTDGDGLSDGDEVNVHGTDPLNPDTD
                     GDGLSDGDEVNV"
BASE COUNT          385 a          667 c          728 g          520 t
ORIGIN      
        1 gaaatggact gcagccagcc accagccggc gaggatcagc cacaacacgt tgcccaggcc
       61 gcgcgcgatg ggtaaccagg tgctgttctg ggcgtccacg tactcctggt gggacgatct
      121 ctcggcccct tcgagtcggt cgaagtgaaa tgcgattcgc ttcacatatc cgtcgaagtc
      181 gttctggtgc ccccgaatct ctcgtttcct atacatcgat cactagttag tccggacgtg
      241 agacagattc cgggccgtct gggtacgtca gtagaggatg tcgagggaga agcgaagggc
      301 ttggtcgagt ttggctcgag tgatttcgtc gaggctccca acgggccgag ggtcgagtag
      361 ttgtttgccg attgtgatga tgttgtcgca gttgatcacg gcgtcgtccg gcaggccctg
      421 ggcaggtcct acctccactt cggacgggat tccccgaacg gttcgggtga tgggggcgca
      481 ggtcacccgg tcgaggactg agatgacact gttgcgggtg aggacacaga ccggacgtcg
      541 tcctccctct tcgccgagat ctgcccagta gatgtcgttg cgggctacca ctcgggtgcc
      601 gtctcggccg ccagtctctc agacgcctgg agcaggagag ggtccggtgg gtacttcgtg
      661 tagccctcga ccagccgctt gtcggcttga gcccactcgt ctgcttcgat gactgactgt
      721 gctgcccggc ggagaagttc ggatcggctg acctgggttc gttgtgcaat ctcgtccagc
      781 ctctcgacaa gttcgtcgtc gagctggacg agcacctcgc gtcttggcat gaccatatgc
      841 taaaccatat ggtccagggt gggtcgccgt ctagaggggt cgtcagtctc cgtgacgtcg
      901 gccgccgccc gggctggatt cccgcaggat cgcctcctat gcagcattag gttttcgact
      961 cccgatattt tcatttgtca ccgagagtgg cgacttgcta cattccgtga ttgaagcggg
     1021 ggacgaggtt atcggttccg tgtggcggcc tcctgttttg gaaaccgccc gtaatcgaaa
     1081 ggaaccgaaa tggcccgctc aatggccttt ctcctgttcg tatcgatcct cgtattgccc
     1141 ttgccggcga gtgcgttgga cgtcacccag tccgaacacg ttcaactcgg cttcaccgcc
     1201 gagttgccga tcagcgagac gatcagcgaa tcctgttgtc tggacacagg ggagtacacc
     1261 gtcgagttcg acggtgaggc caccctcatg gtcgacatgg gcgcggacct cgaggtcacc
     1321 tacgacctgg ccgatctggt ggatgggggg agcatgccca tcgcgctcga gtacaccccg
     1381 accaacgacg ctggaccgga gttgactctc gatgtcagcg gggatttcgg cctcgacttc
     1441 tgtctcctgc tgatcatctg tgatggggac accgtttccg atgtgacgtt tgtcgccggg
     1501 gcaggtgact tcacagctcc gctggccgga gacgcacctg tcctcatacc gctgtcgagt
     1561 gacactctcg aattcttcgt aggtccgttc aaagtcgctg acgctcatat cgagggggat
     1621 ctctcactgg caccagtgcc gtcgggctcc ttccctggcc ttggtggtgc agctgccgga
     1681 ttggccgtcg gtggttcggg aaccctcacg gatccggctc cgatccccat ggtcgaatgg
     1741 cagacggccg ggagccagcc ggggaccgtc ctcctcgatg atcctctcgg cggagaggtc
     1801 gacctgacgg catcaccggt cgtccactgg ctggcgccgt ctttggacgt cgatctggtg
     1861 gtcgatctcg tcgacgagat caacttcttc ggcttcgacg acgtcaccga gtcgctcatt
     1921 tctggtgatc tcggctctga gctccaagct gccgggctcg acacattggt ggctgacgcc
     1981 atcggtggtc tcgccggagc ggcggtgggt agtcgcatcg cggcgggttt cataccgata
     2041 ccgctgctcg atccggctct cgatacgatc tctctcggtt cgactccaac gatcgggtcg
     2101 gtcgagttcg tgatcgacac cgattcggac gacgacggcc tgaccgacgg tgaggagatc
     2161 gccatcggaa ccgatccgtt cgatcccgat accgatggtg atgggttgag cgatggtgat
     2221 gaggtgaatg tccatgggac tgatccgttg aaccctgata ccgatggtga tgggttgagc
     2281 gatggtgatg aggtgaatgt
//
LOCUS       DASUTA010000262         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_462224, whole genome shotgun sequence.
ACCESSION   DASUTA010000262 DASUTA010000000
VERSION     DASUTA010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462224"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /locus_tag="VFZ80_05235"
     CDS             <1..1355
                     /locus_tag="VFZ80_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6286867.1"
                     /translation="TRIELTLEAVELIPQGDDFQTCDFLSEGEEPDLDETCFKAEAVV
                     VLQDGVGLLHRVSLGAVPVNTGVERLSAPLVHTTEAGDLIPPEYPLRLVAIELRSRLP
                     ELSSRQVEMSLSAISVDDGGTLTTIAVDFDDPAWVAHSETFGGLETGPSITRLPDSED
                     RLVVAIETGTGFIGAGSYFGLRPSGSQQIDSIPVVVSSDLAGALAVEPGDSFRMTPLR
                     VPVDSTIVGTFDALPTTAPSVRSTVVLDLPTYQALSYALGEPLPEPEEHWISTSGSSD
                     EVAMALRTEPFNSLSVDSQEELTDTLTGDPVALATIGALTVGFVAAAVFSVVGFAVTA
                     TVSARERLVEFALIRALGMSRRQLGGWLSLEQGALVVVSLALGTLVGVILTAVLLPLV
                     ILTQSGAIPVPEVIVIYPWGTILALELAVVAALTIVVIVMTLLLRRVGLGALLRMGED
                     "
     gene            1373..>2091
                     /locus_tag="VFZ80_05240"
     CDS             1373..>2091
                     /locus_tag="VFZ80_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286868.1"
                     /translation="MSLGARGILSRLVGQPSIPLTIGLTALLATFLLGIAPRVLENVS
                     AEDLRATVSEPHPALRNLRVERQTELTAGSDEDPLSTARSAGMTFAETEFPDSVSSVI
                     SGSYVLVDWPRMVVEALPGEDPPHPFEMLLRLRFQEGLDEHSQLVAGTEPAFHPPIEM
                     LVGEDCPEDPDERNVLAEGLSTGEILPSDDFDCRIDEVPVLEAVFTRETLEAMGLAVG
                     SEMMLTPDLQDPLYFGVPGDRL"
BASE COUNT          352 a          683 c          652 g          404 t
ORIGIN      
        1 cgacccgcat cgaactcacc ctggaagcgg tcgagttgat ccctcagggt gacgacttcc
       61 agacctgtga tttcctttcc gaaggcgagg agcccgatct cgacgagacc tgtttcaagg
      121 ccgaggccgt ggtggtgctt caggacgggg tcggcttgtt gcaccgggtc tccctgggcg
      181 cagttcccgt caacacaggt gtcgagcgcc tgtcggcccc tctggtgcac acaaccgagg
      241 caggcgatct catccctccg gaatatccgt tgcgtctcgt cgccatcgag ctccgcagtc
      301 ggttgccgga gttgtcatcg cgccaggtgg agatgtcgct gtcggcgatt tcggtcgacg
      361 atggcggcac gctcacaacc atcgcagtcg atttcgatga tcccgcctgg gtggcccata
      421 gcgagacctt cggtggtctc gagacaggtc cctcgataac gagactcccc gacagcgagg
      481 atcggctcgt cgttgcgatc gaaaccggca cgggcttcat cggcgcagga agctacttcg
      541 ggctgcgccc cagcggctcc caacaaatcg actcgatccc ggtggtcgtc tcatcagatc
      601 tggccggggc gctcgctgtc gaacctggcg attcgttccg gatgaccccg ctccgggtac
      661 cggtcgactc aacgatcgtt ggcacattcg acgcgctccc cacgacagcc ccaagcgtgc
      721 gcagcaccgt ggttctcgat ctccccacct atcaggctct cagctacgcc cttggcgaac
      781 cgctgcctga gccagaagaa cattggatct cgacatcagg atcgtccgac gaggtggcga
      841 tggcgctgcg aacagaaccc ttcaacagtc tcagcgtcga cagccaagaa gagctcaccg
      901 acaccctcac cggcgacccc gtggctctgg caaccatcgg cgcgctgacc gttgggttcg
      961 tcgctgcggc cgtcttctcc gtcgtgggct ttgccgtcac cgccacggtc tcggcacggg
     1021 aacgactcgt cgagttcgca ttgatcagag cactgggcat gtcacgacgc cagcttggcg
     1081 gctggctgag cctggagcag ggagccctgg tcgtggtcag cctggcattg ggaaccctgg
     1141 tcggggtcat cctgacagca gtgctgctac ctctcgtgat cctcacccag agcggagcga
     1201 taccggtgcc cgaggtcatc gtcatctacc cgtgggggac gatcctggct ctcgagctcg
     1261 cggtggtcgc cgcattgacc atcgtcgtca tcgtgatgac tctcctgctg cggcgagttg
     1321 gcctcggtgc acttctcagg atgggagagg actgatgaac cgccgtggct cgatgagtct
     1381 cggagcacga gggatcctct cccgcctggt aggccagcca tccattccgc tgaccatcgg
     1441 gctcaccgcg ctcctggcga cgttcctcct cggcatagca ccgcgcgtct tggagaacgt
     1501 gtcggccgag gacctgcgag ccacggtgag cgaaccgcat ccggcgctac ggaaccttcg
     1561 cgtggaacgt cagaccgagc tcacagccgg ctccgatgaa gatccgctgt cgacggcgcg
     1621 cagcgccggg atgacattcg ccgagaccga gttcccggat tcggtttcct cggtgatctc
     1681 cgggagctac gtactcgtcg actggccccg catggtcgtt gaggcactgc ctggggagga
     1741 ccctccgcac ccattcgaga tgcttctgcg tcttcgcttc caggagggcc tcgacgagca
     1801 ctcgcagttg gtcgcaggga ccgaaccggc gtttcaccct ccgatcgaga tgctggtggg
     1861 ggaagactgt cctgaagacc ccgatgaacg caacgtgctc gcggagggtc tgtctacagg
     1921 ggagatcctc ccgagcgacg acttcgactg ccggattgac gaggtcccgg tcctggaggc
     1981 ggtgttcacc cgggagacgc tggaggcaat gggtctcgca gtcggctcgg agatgatgct
     2041 gacacccgac ttgcaggacc ccctctattt cggagtcccc ggagatcgtc t
//
LOCUS       DASUTA010000263         5577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_593560, whole genome shotgun sequence.
ACCESSION   DASUTA010000263 DASUTA010000000
VERSION     DASUTA010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593560"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..2781
                     /gene="ppdK"
                     /locus_tag="VFZ80_05245"
     CDS             49..2781
                     /gene="ppdK"
                     /locus_tag="VFZ80_05245"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX6286869.1"
                     /translation="MSEKWVYGFDEVDDAQEAVGGDWDAVRGLLGGKGANLGDMTRIG
                     VPVPPGFTITTAACNAYLDSDGQFPPDLIHQVTAALSDMEAKTGKSFGDPVNPLLVSC
                     RSGAKFSMPGMMDTVLNIGLNDKVVDGLIELTGDEHFVYDSYRRLVQMFGSVVLGVRD
                     EPFEHVLEQRRARAGVDSDSDLSVEDFREITVAFKEIVQRFSGRTFPDDPVDQLELAI
                     EAVFRSWSGKRAIDYRNAAGIPHDLGTAVNVQSMVFGNMGSDSATGVAMSRNATTGEP
                     RLEGDYLTNAQGEDVVAGIRVTKPMEELATEMPELYESFAEIASRLEKHYRDMQDMEF
                     TIERGELWILQTRDGKRTAQAAVRVAVDMSDEGLITREEALMRVLPSQIDFFLHPSFV
                     PESKREAIDAKRVLATGLNVSPGAAVGAVAFDADLAERWAQDEGRKVIMVRPETKPDD
                     VHGMLAAQGILTSRGGRTSHAALVARQFGKPAVVGAADLVIDLSERQMRVGDRIVKEG
                     EIISVDGTTGEVLVGEVETQTPDLENSWLKRLLEWADEVRDIGVWANADYPDDARLAR
                     SYGAEGIGLTRTEHMFFDQARLPIVQRMITATVPADRTEALEQLLPFQREDFAGLFRA
                     MDGLPVTIRLIDPPLHEFLPSFEDLTTNLTDLKIRIRSAGSLEEIDELLAQVEEAEHM
                     LRRVDSLREANPMLGLRGVRLGILMPGLTRMQVRAIFEAACQVADEGFEVHPKVMIPL
                     VAHVNELVRQRAALEEEARTVMSERGIEIEYEFGTMIETPRAAITADEIARHAEFVSF
                     GTNDLTQMTFGISRDDAEKAFLVYYFEEGILDKNPFATIDVNGVGELMRVAIEKGKSE
                     RPELNTGVCGEHGGDPESIALCRRLGLDYVSCSPPRVPIARLAAAQAAIREPAA"
     gene            2765..3400
                     /locus_tag="VFZ80_05250"
     CDS             2765..3400
                     /locus_tag="VFZ80_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286870.1"
                     /translation="MSRLPDPSEPADRSADEARVIVHWIPLGAGGAAAVRISGRIYEG
                     MRAVTEGRRPRDLYHAALEIVLSGERFTIENAWPCPNADVASRGVVLEGPVWSPHLCR
                     LRMFRYEVRCWKDGTIPDIDHAVASPTLTDNADHARRILEAANSIPRLTWGRRAAIGG
                     EMWTSNSVISYLLTVSGVSLSTCTPPGGGRAPGWDVGVCVGRDRAPVLVST"
     gene            complement(3409..3768)
                     /locus_tag="VFZ80_05255"
     CDS             complement(3409..3768)
                     /locus_tag="VFZ80_05255"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6286871.1"
                     /translation="MSLESQLLEIEERFWQSGGDTEFWATYFNDEGVVALPMGLMEKT
                     TVIQSQENARPWNDFSIKDDHVVDLGDDVASISYTVAGRRDGEPDYSAVVTSVYAKRN
                     GEWQLMVHQQTPTDDLS"
     gene            complement(3864..4778)
                     /locus_tag="VFZ80_05260"
     CDS             complement(3864..4778)
                     /locus_tag="VFZ80_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX6286872.1"
                     /translation="MLGRNERLLHLVVDPQSNGGTLRIVITGASGQLARGVIDNALET
                     VSASDLILVSRSPDQLEPYLARGAEVRFGDFEQPESLPDAFQGGTRMLLISTDAVGDR
                     VDQHAAAIDAAVGAGMGLIAYTSMVNPVPENPAFVIPDHRATEQKLRDCGVGWVFLRN
                     SMYAEFQASSMAAAAASGILVSNEGSGQVAYVSRDDCAAAAASVLVSGTHAGSAYDIT
                     GPELLGAEGRAGIFSSITGKSIEVVDVDDDAYAQGLADATGMPLSAARGIASFGEAAR
                     RGFLAVASSDFETLTGRKPRDLRLVLTA"
     gene            complement(4762..5316)
                     /locus_tag="VFZ80_05265"
     CDS             complement(4762..5316)
                     /locus_tag="VFZ80_05265"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX6286873.1"
                     /translation="MTNQYVALLRGIGPSNPSQRNENLRRVSEELGFHNVQTVISSGN
                     VLFESDWNDSSGIESEMEGAWKDHLGFHSLTIVRSRAELQQLVELAPFGDREHGPETY
                     LLATFGKTRLEHAWEFPFQPEGKDYWVVAGTDREIFTVTDTVHSRTPDVMSWVETQFD
                     SGVTSRTWLTVLRILKRMTDARPE"
     gene            5377..>5577
                     /locus_tag="VFZ80_05270"
     CDS             5377..>5577
                     /locus_tag="VFZ80_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HEX6286874.1"
                     /translation="MDFRRIGSLPPYVFATINELKLDARRRGEDVIDLGFGNPDIPSP
                     PEAVEKLREAALNPRNHRYSSSR"
BASE COUNT         1053 a         1695 c         1789 g         1040 t
ORIGIN      
        1 aaggtttctc gagtcgaatc ctccgctgtg acaacgaaga ggttcgagat gtcggagaaa
       61 tgggtttacg gattcgatga ggtggatgac gcccaggaag ccgtcggtgg agactgggac
      121 gcggttcggg ggctgctcgg gggaaagggg gccaaccttg gtgacatgac ccgtatcggg
      181 gttcccgttc ctcccggatt cacgatcacg acggccgcct gcaacgccta cctcgactcc
      241 gacggtcagt tccctcccga cctgatccac caggtgaccg cggcgctctc cgacatggag
      301 gccaagaccg gaaaatcgtt tggcgatccg gtcaacccac tgctggtctc ctgcaggtcg
      361 ggagccaaat tctcgatgcc ggggatgatg gacaccgttc tgaacatcgg cctgaacgac
      421 aaggtggtcg acgggttgat cgaattgacc ggtgacgagc atttcgtata cgactcctac
      481 cgacgtctcg tgcagatgtt cggttcagtc gtgcttggtg tcagggacga gccgttcgag
      541 cacgtcctgg aacaacgacg cgccagggcc ggcgtcgaca gtgactccga cttgagtgtg
      601 gaggacttcc gtgagatcac cgtcgcattc aaagagatag tccagcgctt ctccggccgc
      661 accttccccg atgacccggt cgaccagctg gagcttgcca tcgaggcggt gttccgttca
      721 tggagcggca agagagcgat cgattacagg aacgcagccg gcatacccca cgacctgggg
      781 accgcggtga acgttcagag catggtgttc gggaacatgg gctccgattc cgccactgga
      841 gtcgccatgt cacgcaacgc caccaccggt gagccccgtt tggagggcga ctatctgacg
      901 aacgcccagg gggaggatgt ggttgccggc atccgtgtca ccaagccgat ggaggagctc
      961 gcaaccgaga tgcccgagct gtacgagtcg ttcgcagaga tagcgagcag gctcgagaag
     1021 cactaccggg acatgcagga catggagttc accatcgaga gaggggagct ctggatcctt
     1081 caaacgcgcg atgggaaacg aactgcacag gctgcggtcc gggtcgccgt cgacatgtct
     1141 gacgaaggcc tgataacccg cgaggaggcc ctgatgcggg ttttgccctc acagatcgac
     1201 ttcttcctcc atccgtcgtt cgtcccggag agcaagcgcg aggcaatcga tgcaaagaga
     1261 gtcctggcca ccggtctcaa tgtctccccg ggcgccgcgg tgggcgcagt ggcgttcgac
     1321 gctgatctcg ccgagagatg ggcgcaggac gagggccgca aggtgatcat ggtcaggcca
     1381 gagaccaagc ccgacgatgt gcacggcatg ctggctgctc aggggatact caccagccgc
     1441 gggggccgga ccagccatgc cgccctggtg gcccggcagt tcgggaagcc ggccgtggtg
     1501 ggtgctgccg acctggtcat agatctcagc gagcgacaga tgcgggtggg agatcgcatc
     1561 gtcaaggaag gcgagatcat ctcggtcgac gggactacag gcgaggtcct cgtcggtgag
     1621 gtcgagaccc agaccccgga cctcgagaac tcctggctga agagactgct ggaatgggcc
     1681 gacgaggtga gggacattgg tgtctgggcc aacgccgatt accccgacga tgcccgtctg
     1741 gcccgcagct atggggcgga agggataggt ctcacccgaa ccgagcacat gttcttcgat
     1801 caggctcggc tgcccatcgt gcagcggatg atcacggcga cggtccccgc cgaccgcacc
     1861 gaagccctag agcagctact gccgtttcag cgagaggact tcgccggtct cttccgagcc
     1921 atggatgggc ttccggtgac gatccgcctc atcgaccctc cgctgcacga gtttctcccc
     1981 agcttcgagg atctgaccac gaatctgaca gacctcaaga tccgaattcg atctgctggt
     2041 tccctcgagg agatcgacga gctcctcgcc caggtagagg aagcggagca catgttgcgt
     2101 cgtgtggaca gcttgcgcga ggcgaacccc atgctcgggt tgaggggagt ccgactcgga
     2161 atcctcatgc ccgggctcac ccgtatgcaa gttcgagcca ttttcgaggc ggcctgccag
     2221 gtcgccgatg agggtttcga agtccatccg aaggtgatga tccccctggt cgctcatgtc
     2281 aacgagctcg tcagacagcg cgcggccctg gaggaggagg ccagaaccgt gatgtcagag
     2341 cgggggatcg agatcgagta cgagttcgga accatgatcg agacaccgag ggctgctatc
     2401 accgcggacg agatcgccag gcatgccgaa ttcgtgtcgt tcggcaccaa cgatctgacc
     2461 cagatgacct tcggcatcag ccgcgacgat gcggagaagg ccttcttggt ttactacttc
     2521 gaagaaggca tcctcgacaa gaacccgttt gccacgatcg atgtgaacgg ggtcggcgaa
     2581 ctgatgaggg tggccatcga gaagggcaaa tcggaacggc ccgagctcaa caccggcgtg
     2641 tgcggtgagc acggtgggga cccggagtcg atagcgctct gccgtcgtct cggtctcgac
     2701 tacgtgagtt gttcgcctcc cagggtgcca atcgcccgtc tggcagcggc gcaggccgcg
     2761 attcgtgagc cggctgcctg atccctcgga gcccgccgat cgttcggcag acgaggctcg
     2821 tgtcatcgtc cactggattc cgctgggggc gggaggggct gcggctgtga ggatcagcgg
     2881 acggatctac gaaggcatgc gggcagtcac cgagggacga aggccacgcg atctctatca
     2941 cgccgcgcta gagatcgtcc tctcgggtga gcgtttcacc atcgagaacg cctggccgtg
     3001 cccgaatgcc gacgtcgcct cgaggggtgt cgtcctcgag ggtccggtgt ggagccccca
     3061 cctgtgccga ctcaggatgt ttcgatatga ggtccggtgc tggaaggacg ggaccatacc
     3121 cgacatcgat catgccgtag ctagcccaac tctgaccgac aacgcggatc atgcccgtcg
     3181 aattctcgaa gccgccaact ccatcccacg actcacatgg ggacgcaggg cggccatagg
     3241 cggggaaatg tggacgtcca actccgtgat ctcctacctg ctcaccgtct ctggggtgtc
     3301 gctatcgacc tgcacgcccc ctgggggtgg tcgtgcgccc ggctgggatg tcggcgtgtg
     3361 tgtgggcagg gaccgggcac cggtgttggt ctcgacctga gtcggagatc aactcaggtc
     3421 atctgttggc gtttgctggt ggaccatgag ctgccattca ccgttgcgct tggcgtagac
     3481 gctggtcaca accgctgagt agtcgggttc gccatcgcgt cgcccggcga ctgtgtaact
     3541 gatcgacgct acgtcgtccc cgagatccac gacatgatcg tctttgatcg agaagtcgtt
     3601 ccagggtcgt gcattctcct ggctctgaat gacagttgtc ttctccatca ggcccatggg
     3661 gagagcgaca acgccctcgt cgttgaagta cgtggcccag aactcagtgt cgccaccgga
     3721 ttgccagaaa cgctcctcga tctcgaggag ctgtgattcg agtgacatca agcctccttc
     3781 cgcaggtgag cgcgtcatac ccaatgaaga gcgccttcac acggtgtcga gaccagccag
     3841 tcttcggcca ccccggcggg ccctcaggcg gtgagcacca agcgcagatc ccggggcttg
     3901 cgtcccgtca gggtctcgaa gtcggagctc gccactgcga ggaacccccg gcgagccgcc
     3961 tctccaaagc tggctattcc gcgggcggcg gaaaggggca ttccggtggc atcggcaagc
     4021 ccttgggcat acgcgtcgtc atcgacatca acgacctcga tcgacttgcc ggtgatcgac
     4081 gaaaagatcc ctgcccgtcc ttcggcgccg agcagttccg gcccggtgat gtcatacgcc
     4141 gagccggcat gggtacccga caccaggacc gaggcggccg ccgcggcgca atcgtcgcgc
     4201 gacacatatg caacctgtcc ggatccctcg ttgctgacga ggatgccgct ggcggccgcg
     4261 gccgccatcg aggaggcctg gaattccgcg tacatcgagt tgcgaaggaa gacccatccc
     4321 actccgcaat cacgcagctt ctgctcggtg gcgcgatggt ccgggatcac gaaggccgga
     4381 ttctcaggca ccgggttgac catggacgtg taggcgatca gtcccatgcc ggcgccgacc
     4441 gcggcgtcga tggcagctgc gtgttgatcg acgcgatcac caactgcgtc ggtcgagatg
     4501 aggagcatcc gagtcccccc ttggaaggca tccgggagtg attccggctg ctcgaagtct
     4561 ccgaaccgga cttcggcccc gcgagccagg tatggctcga gctggtccgg tgacctgctc
     4621 accagtatca ggtcggatgc agatacggtc tccaacgcgt tgtcgatgac tcctctggcc
     4681 agctggccgg acgctcccgt gatcactatg cgcaaggtgc ctccattgct ctggggatcg
     4741 acaacgaggt gcaggagtcg ttcattccgg ccgagcatcg gtcatccgct tcaggattcg
     4801 aaggaccgtg agccatgttc gcgatgtgac accactgtcg aactgagtct ccacccagct
     4861 catcacatcg ggtgtccggc tgtggacggt atcggtgaca gtgaagatct cacggtcggt
     4921 tccggcgacg acccagtagt ccttgccttc aggttgaaag gggaactccc aagcgtgctc
     4981 gaggcgagtc ttgccgaagg tggccaggag ataggtctcc gggccgtgct cccgatctcc
     5041 gaagggcgcc aactcgacca actgctgcag ttccgcgcgg ctgcgcacga tcgtgagact
     5101 gtggaacccg aggtggtcct tccaggcacc ctccatctcc gactcgatcc ccgatgagtc
     5161 attccaatcc gactcgaaga gcacgttccc gctggaaatg accgtctgga cattgtgaaa
     5221 accgagctcc tcgctgaccc ggcgcaggtt ctcgttcctc tggctcgggt tggagggccc
     5281 gatgccgcga aggagggcga catattgatt ggtcatgacc gtgggtccgc caattgcgtt
     5341 ggtggctcgt cgtgcggcca ctaaacctag gtggacatgg attttcgtcg catcggatcg
     5401 ctccctccct acgtgttcgc cacgatcaac gagttgaagc tcgatgcgag acgtcgtggg
     5461 gaggacgtca tcgatctcgg tttcgggaac ccggacatcc cttctcctcc ggaagctgtg
     5521 gagaagctgc gggaggcagc cctcaatcct cgcaaccatc ggtactcgtc ttcgcgg
//
LOCUS       DASUTA010000264         3834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1759710, whole genome shotgun sequence.
ACCESSION   DASUTA010000264 DASUTA010000000
VERSION     DASUTA010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759710"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VFZ80_05275"
     CDS             <1..508
                     /locus_tag="VFZ80_05275"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6286875.1"
                     /translation="NSLESDLMPRTSLSHLECSVANTVEVVRDSWTVLVLRDAFQGVR
                     RFDQFVESLGIARNTLASRLEALVEAGVMRAEPYQDNPVRYEYRLTDKGKDLFDVLMT
                     LWAYGERWNPPERADRQNAIHLDCGHPADAVAHCSHCGQRITRHNIRIEPPNEVIART
                     EAKAASAG"
     gene            complement(509..3685)
                     /locus_tag="VFZ80_05280"
     CDS             complement(509..3685)
                     /locus_tag="VFZ80_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEX6286876.1"
                     /translation="MGALNGVQPDSPVISTPDQRLRVFVSSNLSELGEEQAAVKSAIT
                     AMRLTPVTFEPGVSPHPQAEVYRRYVEQSDVFVGIYWESYGWSAPGAEISSIEDELDL
                     AAGRPRLVYVKEPAPGREPALRKMLDRVQYQSSAIVRTFSGTEELAALVVDDLAVLVT
                     ERFHTYDDESHLASGALTFFFADLEGSTGMLQRLGDGYQRVLGTYHRIVNDSVRLHHG
                     HVVITEGDGFFCVFSGPREAMEAAHDIQVAMSEGPWPRNERPRCRIGIHTGTAARTME
                     GYVGLDVHVAARIGDAAQGGQIIVSAATADLVAEDAAGRGWTIVDLGQYDLRGVGHSE
                     RLHRIDMPGLDAVRTAPRARPKIPSTVPGTPKRLIGRRDDLRGAIEMIMRDMVRLVTL
                     TGPGGTGKTRLAIEIARSLEERFPDGVVYVDLSAVRDPERFMPVVGRALGVRESADRT
                     IAAALASVIGDGRMLVVIDNVEQLLPAVSSQVAELIEAMPGVKFLVTSRSPLRIAWEH
                     EYPVPPLEVPEPGDEDETVDGAPAVTLFVERAKAARPLFEMNEVTRPVVVDVVRRLDG
                     LPLAIELAAARLRMFSVEELEQRLDDRLNILDRGSADSPERHRTLRAAIGWSYDLLSD
                     DERLLFRRLAVFSGGWTLAGAVAVCCDDEFSEPRVLDSMEELVAKSLVVFTINEEGRP
                     RYRLLETLREFAVEQLRASGEEEQIRLRHLDWLQGVAERVLIVLPTPEFPSFLDDVER
                     ERFNIREGLAWAVRHKMGTEQALRVCGMLPLFWDTRGFVAEGLRWTRALVAMTTSQGN
                     TEARGMAHTAMGWLEMLAGEADESEWALATAERMFREMGNDNWLGRTLSMRGMTTYNR
                     GLLDEAEEQFNEAIVLCRESGLDWLADAWCTYGLAHVAMSRGDFGAAEPLLLHCFEYS
                     KVNGLSWGVGHTQLSLSVMAFMLGDLEQSVERMIESIKVRAELRDSRGLCDCIGMMAL
                     HASVRGDHDLAATLIGAAEVAREASGDHLVPWQQPLLEQAVASAKANLGDDYEVRFME
                     GRRLTMSESISLILDRFESSVERSAVSA"
BASE COUNT          721 a         1275 c         1173 g          665 t
ORIGIN      
        1 aaactcacta gaatctgatc tcatgccaag aacctcgctc tcgcatctgg agtgctcggt
       61 cgccaacact gtcgaagtgg tccgcgactc gtggacggtt ctcgtcctgc gcgatgcctt
      121 ccagggcgtg cggcggttcg accagttcgt ggagagtctc ggcatcgccc gcaacaccct
      181 ggccagccgt ctcgaggcct tggtcgaagc aggggtgatg cgagcagagc cataccagga
      241 caacccggtc cgatatgagt accggctgac tgacaaaggc aaagacctct tcgacgtcct
      301 catgacgctt tgggcgtatg gcgagcgctg gaacccaccg gagcgggccg accgccagaa
      361 cgcgatacac ctcgactgcg gtcacccggc cgacgccgtt gctcactgca gtcactgtgg
      421 gcagaggatc acccgtcaca acatccgcat cgagccgcca aatgaagtca tcgctcgaac
      481 ggaagcgaaa gccgccagcg ccggttgatc aggcggagac cgccgagcgc tcgacagacg
      541 actcgaagcg atccagtatc aacgagatcg attcgctcat ggtgagacgg cggccctcca
      601 tgaacctgac ctcgtagtcg tcgccgagat tggctttggc cgaggcgacg gcctgttcga
      661 gaaggggctg ctgccaggga acgagatggt caccagaggc ttccctggcg acttcggcgg
      721 cgccgatgag ggtggcagcg aggtcgtgat cgccacgcac cgaagcatgc agcgccatca
      781 tcccgatgca atcgcaaagg ccgcggctgt ccctgagctc ggcgcgcacc ttgatgctct
      841 cgatcatccg ctcgacggac tgttcgaggt ccccgagcat gaaggccatg accgacaggc
      901 tcagttgggt gtggccgact ccccaggaga gcccgttgac cttggagtac tcgaagcagt
      961 gaagtagcaa cggctccgcg gcgccaaagt cacctcggga catggccacg tgtgccaggc
     1021 cgtaggtaca ccatgcgtcg gccagccaat cgagcccgct ctcgcggcag aggacgatgg
     1081 cctcgttgaa ctgctcttca gcctcgtcga gcagcccacg gttgtaggtg gtcatccccc
     1141 gcatcgacaa agtccgtcct agccagttgt cgttgcccat ctcccgaaac atccgctcgg
     1201 ccgtggccag agcccactcc gactcgtcag cctcgcccgc cagcatctcg agccagccca
     1261 tggcggtgtg ggccatgccc cgggcctcgg tgttcccctg cgacgtggtc atggcgacca
     1321 gggctctcgt ccagcgaaga ccttcggcga cgaacccgcg ggtgtcccaa aacaggggca
     1381 gcatcccgca tacgcgcagg gcctgctcgg tgcccatttt gtggcggacc gcccaggcca
     1441 ggccttcgcg gatattgaat ctctcacgtt ccacgtcgtc gaggaagctc gggaactcag
     1501 gtgtgggcag gacgatcaag accctttcag ccactccctg aagccaatcg aggtgacgga
     1561 gcctgatctg ctcctcttca ccacttgcac gtagctgctc gacggcgaac tctcggaggg
     1621 tctcgagaag acgatatctg gggcggccct cctcgtttat cgtgaacacg accagcgact
     1681 tggccaccaa ctcctccatg gagtcgagca cccttggctc gctgaactcg tcgtcacagc
     1741 agacggccac cgccccagcc aaggtccagc caccggagaa caccgcgagc cggcggaaca
     1801 ggagccgctc gtcatcgctg aggaggtcat agctccaccc gatcgcggca cgcaacgtcc
     1861 ggtgcctctc gggtgagtca gccgaccccc ggtcgaggat gttgagacga tcgtccagcc
     1921 gttgctcgag ctcctccacc gagaacatgc gcagtcttgc cgcagccaac tcgatcgcca
     1981 gcggcaggcc atcgagccgc cgcaccacat ctacaacgac gggccgagtc acctcgttca
     2041 tctcgaacag tgggcgggcg gctttggccc tctcgacgaa gagcgtgacg gcgggggcgc
     2101 cgtcgacggt ctcgtcttcg tcgccgggct cgggtacttc gagtggcgga acgggatact
     2161 cgtgctccca ggcaatgcgc aggggcgatc gactggtgac gagaaacttc acgcctggca
     2221 ttgcctcgat gagctcggcg acctgactgg agactgcggg caggagctgc tcgacgttgt
     2281 cgatcacgac gagcatccgt ccatcaccga tgaccgacgc cagtgccgcg gcgatggtgc
     2341 gatctgccga ctcccgaacc cccagggccc tcccaacaac gggcatgaac cgctccgggt
     2401 cccgcaccgc ggagaggtcg acgtacacca cgccgtcggg gaaacgctct tccaacgacc
     2461 tggcgatctc gatggccagc ctcgtcttgc ccgtcccacc ggggcccgtc agtgtgacca
     2521 gacgaaccat gtcacgcatg atcatctcga tggcgccgcg gagatcgtcg cgcctcccga
     2581 tgagccgttt cggtgtgccc gggaccgtcg aggggatctt cggtctcgct cgtggcgccg
     2641 tccgcaccgc atcgagcccc ggcatatcga tccgatgcag gcgttcgctg tgccctacgc
     2701 ccctcaggtc atattgaccg agatcgacga tggtccaacc tcgcccggcc gcatcctcgg
     2761 cgaccagatc ggcagttgcc gccgacacga tgatctgtcc gccctgggct gcgtctccga
     2821 tacgtgctgc cacgtgcacg tcgaggccga cgtacccctc catcgtcctg gcggcagtgc
     2881 cggtatggat cccgattcga cagcgaggcc gctcgtttcg gggccagggc ccttcgctca
     2941 ttgcgacctg gatgtcgtgg gcagcctcca tcgcctctcg ggggcccgag aacacacaga
     3001 agaagccgtc tccttcggtg atgaccacgt gcccgtgatg aagtctcacc gagtcgttga
     3061 ctatgcggtg ataggtgccg agtacccgct gatagccgtc ccccaaccgt tgaagcatgc
     3121 cagtggaacc ctcgagatcg gcgaagaaga aggtgagggc cccagaagcg aggtgggact
     3181 cgtcgtcgta cgtgtgaaaa cgctcagtga ccaggactgc gagatcgtcg acgacaagag
     3241 ccgccagctc ctcggtgccg ctgaacgtcc gcacgatcgc cgagctctga tactggactc
     3301 ggtcgagcat cttccgcaag gcgggctccc gtcccggggc cggctctttc acgtagacga
     3361 gccgtggcct tccggcggcc aggtcgagct cgtcttcgat gcttgagatc tcggcccccg
     3421 gtgcgctcca accgtatgac tcccagtaga tgccgacgaa gacgtctgat tgctcgacgt
     3481 accgtcgata gacctccgcc tgcggatgag ggctcacccc gggctcgaac gtgaccgggg
     3541 tcagccgcat agcagtgatc gcggatttga cggcagcctg ctcttcgccg agctcggaga
     3601 gattcgagga gacgaagacg cgaagccgct gatcaggtgt gctgatcacc ggcgaatcag
     3661 gttggacacc gttcaaagca cccatggccc cccgatggga agaaccccgc acggcaacgc
     3721 cggcttcggc gattttacct ctgtttggcc caatatgaga cgccacctac cttgagtaga
     3781 aagtgaggta gatgacttac gtagcgctac tgcgcggaat aaatgtcgga ggcc
//
LOCUS       DASUTA010000265         2177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_982143, whole genome shotgun sequence.
ACCESSION   DASUTA010000265 DASUTA010000000
VERSION     DASUTA010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982143"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1120)
                     /gene="ruvB"
                     /locus_tag="VFZ80_05285"
     CDS             complement(104..1120)
                     /gene="ruvB"
                     /locus_tag="VFZ80_05285"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX6286877.1"
                     /translation="MSREESLLSPTIEDVDEITLRPKKLVDFVGQNQVKERLQISLEA
                     SIAEDRALDHVLFSGPPGLGKTTLAGIIATEKGVNLRITSGPALERPGDLAAILSNLH
                     PHDVFFIDEIHRLPRAVEEVLYPAMEDYQLDVVLGKGPTAQSIRIDLPRFTLVGATTR
                     KGKVTAPLRDRFGIEERLDYYEPQDLARIIKRSAGIIEVVVDPDAAELIAERSRRTPR
                     IANRLLARVRDYAVARASGTITLDVAGEALKVYEVDDLGLDKVDRSILNAIINKFGGG
                     PVGLSTLSIAVGEEPETVEDAYEPFLLQTGLIQRTPRGRVATTHAYAHLGVDPPADAQ
                     GSLL"
     gene            complement(1117..1689)
                     /gene="ruvA"
                     /locus_tag="VFZ80_05290"
     CDS             complement(1117..1689)
                     /gene="ruvA"
                     /locus_tag="VFZ80_05290"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX6286878.1"
                     /translation="MIGRLRGVLVSSREPGITIEVAGVGYEVAMTTRDLASLPGIAEE
                     IVIHTHTHVREDAISLYGFATESDRELFRVLLTASGVGPKVAMTMLASMTGEEIIRAV
                     NGEDPDALTVVPGVGKKGAQKIVLELGNKLAGHEIDVVGSQSLGGVRQALEGLGYSTE
                     EINTVVGELDPAEPIEAQVKSALQRLGQQR"
     gene            complement(1686..>2177)
                     /gene="ruvC"
                     /locus_tag="VFZ80_05295"
     CDS             complement(1686..>2177)
                     /gene="ruvC"
                     /locus_tag="VFZ80_05295"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX6286879.1"
                     /translation="LEHMFVLGIDPGLSVTGYGLVEGSYPPRAIGAGIIRTDTDLTIP
                     QRLAELYSGLGQVVADADPDVIAMETVFTNRNLQTAMSVGRASGIALLVAAEAGLDVH
                     EYVPTAIKAAVTGDGTAKKSQVQQMVARLLGLSEAPKPADASDALAIALCHLRAAPLE
                     VRA"
BASE COUNT          366 a          673 c          692 g          446 t
ORIGIN      
        1 tctgacatgt ctcgggtgtc gaggagcctg gcgtcgtggc ggggctcgat ggcgttctgt
       61 gctatcgccg attgcgggag gtggtagtgg aactcgctcg ctttcagagc aacgaccctt
      121 gtgcgtccgc gggcggatcg actccgagat gggcgtaggc gtgagtcgtg gccacccgac
      181 cacgtggtgt gcgctggatc aacccggttt ggagcaagaa tggctcgtag gcatcctcca
      241 ccgtttccgg ttcctcccct accgcaatcg acagggttga gaggccgacc ggtcctccgc
      301 cgaatttgtt gattatcgcg ttcaggatcg acctgtcgac cttgtcgagt ccgaggtcgt
      361 ccacttcgta taccttcagc gcttcgcccg cgacatccag cgtgatcgtc cctgatgctc
      421 tggccaccgc atagtcccga acccgggcaa ggaggcgatt ggcgatgcgg ggcgttctcc
      481 ggctccgctc ggctatcagt tcggctgcgt cgggatcgac cacgacctcg atgattccgg
      541 ccgacctctt gatgatgcgg gccaggtctt ggggctcgta gtagtcgagc cgctcctcga
      601 tcccgaagcg atcgcggagt ggtgctgtca ccttgccctt cctggtcgtg gccccgacca
      661 gggtgaaacg gggtagatcg atcctgatcg attgcgcggt tgggcccttg cccaggacca
      721 cgtccaactg gtagtcctcc atagcgggat agagcacctc ttcgacagca cgtggcaaac
      781 ggtggatctc gtcgatgaaa aacacgtcgt ggggatggag gttggagagg atcgcggcga
      841 ggtcgcccgg acgctccagc gccgggccgg aggtgatccg gaggttgacg cctttctcgg
      901 tggcgattat cccggcgagc gttgtcttgc cgagacccgg gggacccgag aacagaacat
      961 gatcgagagc tcggtcctcg gctatcgagg cctcgagtga gatctgaaga cgttccttga
     1021 cctggttctg acccacgaag tcgacgagct tcttgggacg aagtgttatc tcgtccacgt
     1081 cctcgatggt cggactgaga agactctctt cgcggctcat cgctgttgac cgagccgctg
     1141 taatgcagat ttcacctgtg cctcgatcgg ttcggccgga tcgagctcgc caaccaccgt
     1201 gtttatctct tcggtggaat accccagacc ctccagggcc tggcggacgc cgcccagcga
     1261 ttggcttccg acgacatcga tctcgtgccc ggcgagtttg ttgcctagct cgagcacgat
     1321 cttctgcgcc cccttcttcc cgactccggg gacgaccgtg agggcatcgg ggtcttcgcc
     1381 gttgaccgcc cggatgatct cctctccggt catcgacgcc agcatcgtca tcgccacctt
     1441 gggaccgact ccactggcgg tgagaaggac gcggaacagc tccctgtccg attcggtggc
     1501 gaatccgtag aggctgattg cgtcttcacg gacatgagtg tgggtgtgga tgacgatctc
     1561 ctcagcgatc ccggggagac tcgccagatc tcgcgttgtc atcgccacct cgtagccgac
     1621 accggccacc tcgatggtta tccccggctc ccggctggag acgagcacac cccgcagacg
     1681 cccgatcacg ctcgcacctc gagtggtgcc gcccgaagat gacacagtgc gatggccaac
     1741 gcgtccgatg cgtcggcggg tttgggtgcc tcggagagac cgaggagacg ggccaccatc
     1801 tgttgcacct ggctcttctt agccgttccg tctccggtaa cggctgcttt gatcgccgtg
     1861 gggacgtatt cgtgaacgtc gaggccggcc tcggccgcga ccagcagggc gattccggat
     1921 gcgcgtccga ccgacatcgc cgtctgcagg ttgcgatttg taaacaccgt ctccatggca
     1981 atgacgtccg ggtcagcgtc agcaaccacc tgaccgagcc cggagtacag ctcggcgaga
     2041 cgctgcggga tggtgaggtc ggtatctgtg cggatgatgc cggcaccaat ggctcggggc
     2101 gggtacgagc cctcgaccaa gccatatccg gtgaccgaga gacccgggtc gatgccaaga
     2161 acgaacatat gttctag
//
LOCUS       DASUTA010000266         3901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1890398, whole genome shotgun sequence.
ACCESSION   DASUTA010000266 DASUTA010000000
VERSION     DASUTA010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1890398"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VFZ80_05300"
     CDS             complement(<1..167)
                     /locus_tag="VFZ80_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6286880.1"
                     /translation="MKPGSSRRLEVRGVTKQFEGQPVLKGVDLTVEKGTSVTLLGPSG
                     CGKTTLLRVIAG"
     gene            complement(313..1896)
                     /locus_tag="VFZ80_05305"
     CDS             complement(313..1896)
                     /locus_tag="VFZ80_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6286881.1"
                     /translation="MAPIVVVGLGATSPSGDVWRQLWGGPLPEMLRTTAVLLILVGGG
                     AIFLGTALAWLTTAYEFPGRRLFTWALILPLAMPAYILGFVFLALFDFAGPVQTSLRS
                     VFGANIWFPDVTSIGGAAVVMTLSLYPYVYILARSALREQTPATYEAARMLGDTRKAA
                     LLRVVLPMARPSLAAGAALVMMEVLTDFATVQYFNVQTVSVGIYQVWRGMFDRETAVE
                     LAALVLLVAVIVIGLERLLRGPARFHQRGSPRDIERVTLHGPRRALAVGACWAVLGAA
                     ILLPIAQLLYWLSTSPAVVTDLGRGVEYLSNSLGLAVTAAVGAVFFALLAAGGSRFSG
                     RPLVRRMVHLITVGYAVPGPVVAIGVLVMIGWVTRATGGSPARLLLLSLTGLVYAYVV
                     RFVALAYGSVDASLEKITPSTVDAAHTLGANPSRVMRQVYFPLAGAGAAAGAVLVAID
                     VLKELPIVLLIRPFGFTTASIWVWERASESLWAAAAIPALLIVLTAMIPVALYLRSER
                     RSRGSALALETRLTSVGES"
     gene            complement(1964..2968)
                     /locus_tag="VFZ80_05310"
     CDS             complement(1964..2968)
                     /locus_tag="VFZ80_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6286882.1"
                     /translation="MTVGGFAFLLIVPVALAACGVVDALDNDQVIGVYTGRHYDLEEA
                     FGEYTEETGVEVDFLTGGDTELRERLRTEGESTLADVFMTVDAANLQLAADEDLLQPM
                     ESEVIESLPDNLVHPEKLWVGLSKRVRTIMYHPDRVSADDLSTYEDLADPMWSGRLCM
                     RDSTNVYTQSLVASLIHHLGYEEALRIVQGWVANETQIMTSDSLILETIAAGGCDVGI
                     TNHYYLARLLAEDPDFPVLPFWANQDGRGVHVNISGAGITSDADDPDLAADLIEWLAT
                     EGQEAFVYGNHEYPVNPDVPPPPIVRDWGEFVEDDLNAAEFASLNADAIRLMSEAGYD
                     "
     gene            complement(3070..3408)
                     /locus_tag="VFZ80_05315"
     CDS             complement(3070..3408)
                     /locus_tag="VFZ80_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286883.1"
                     /translation="MDQMTGEITVQPQPTTDSTQRLFTMSMLISGIRCVLAYVVLPFF
                     TPFLGLAPGVGPVLGLVIGAVAIAANVFSIRRFWRANHRWRKPITVLHVGVIILLLIL
                     MWFDLVELLA"
     gene            3533..>3901
                     /locus_tag="VFZ80_05320"
     CDS             3533..>3901
                     /locus_tag="VFZ80_05320"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX6286884.1"
                     /translation="MGIREGLLALLADGPRHGYQLKLDLEMATGDAVTLNVGQVYTTL
                     QRLERDGLVEDGGTDVEGRAIYRITKPGIAAIREWASRPEDLAAAGRDEISIKVLLAL
                     FTPGLDPRQVIETQRRATMGM"
BASE COUNT          816 a         1219 c         1215 g          651 t
ORIGIN      
        1 ccggcgatga cgcggagcag ggtggtctta ccgcatccgc tcggaccgag cagggtgacc
       61 gacgttccct tttccactgt caggtccacg cccttgagca caggttgacc ttcgaattgc
      121 ttggtgacgc ctcgtacctc gagacgccgg gaggacccgg gtttcacgtg accaatggta
      181 tcaacaggag ccccagaccg taaatgtacg ggaagcctta caccgacctt ccccacatca
      241 tcgagcagga tcagggttcg gctcagcatg acgacgtgca agcctggtgg gcgtcaccag
      301 acgatgaacg agtcacgatt cgcccacgct cgtgagacgg gtctccaagg ccagcgccga
      361 gccgcgactg cggcgctcgc tccggaggta gagcgcgact ggaatcatgg ccgtgagcac
      421 gatgagcagg gccgggatcg cagctgcagc ccagagcgac tcggaggcgc gctcccaaac
      481 ccagatggag gctgtcgtga acccgaacgg gcggatgagt agaacgatgg gcagctcctt
      541 caggacatcg atcgccacca gcaccgctcc ggcggctgcg cccgccccgg ccagcgggaa
      601 gtacacctgt ctcatcaccc gcgacggatt tgcgcccagt gtgtgagcgg catcgaccgt
      661 ggagggcgtg atcttctcca ggctggcgtc caccgagccg taggccaggg ccacgaaccg
      721 aaccacatac gcatatacca ggccggtgag tgagagcaga agaagcctcg cggggctgcc
      781 accagtggct cttgtgaccc acccgatcat cacgagaacg ccgatggcaa ccaccggacc
      841 cggcacggca tatccaaccg tgatcagatg caccatgcga cgcaccaggg ggcggccact
      901 gaaccgggag ccgcccgcgg cgaggagggc gaagaacacc gctccgactg cggctgtcac
      961 cgccaggccc aggctgttcg atagatactc gactccccga cccaggtcgg tgacgaccgc
     1021 cggcgaggta gacagccaat agagcaactg cgcgattggc aacaggatcg cggcgccaag
     1081 gactgcccag caggctccaa ccgccagggc tctcctcggt ccatgcagtg tcacccgctc
     1141 gatatcgcgc ggcgagccgc gctggtgaaa tcgggcgggt cctcgcagga gacgttcgag
     1201 gccgatcacg atcacggcga cgagcagaac cagagcggcg agttccaccg cggtctcccg
     1261 atcgaacatg cctctccaca cctgatagat cccgacggaa accgtttgga cgttgaagta
     1321 ctggaccgtg gcgaaatcgg tgagaacctc catcatcacc agtgccgccc cggcggcgag
     1381 cgatgggcgc gccatgggaa ggacgacgcg gagcaacgcc gctttcctcg tatcgcccag
     1441 catgcgggcc gcctcgtagg tcgccggggt ctgctctcgt agggcggacc gggcgaggat
     1501 gtacacgtag ggatacagcg acagcgtcat gacgacggcg gcgccgccga ttgatgtcac
     1561 atccggaaac cagatgttcg ccccgaacac cgaccgcaac gacgtctgca ccggcccggc
     1621 gaagtcgaac agagccagga agacgaatcc gaggatgtac gccggcatgg ccaacggcag
     1681 tatcaatgcc caggtgaaca ggcgacggcc cgggaattca tatgcggtgg tcagccaggc
     1741 gagggcggtg ccgagaaaga tcgctcctcc tccgacgaga atcagcaaaa cggccgtggt
     1801 ccgcagcatc tcgggtaatg gccctcccca tagttggcgc cagacatcgc cggaagggga
     1861 ggtggcgccg aggcccacga cgactatcgg agccacgatc agcaggacta ccccgagcgc
     1921 tattccagtc cagagagggc ggcgcccgcg ggcgagagtc tgatcagtcg tagccggcct
     1981 cgctcatgag ccgaatcgcg tcggcattca gagaggcgaa ctccgcagcg ttcaggtcgt
     2041 cttcgacgaa ctctccccag tcccgtacta tcggcggcgg cggcacgtcc gggttgaccg
     2101 gatattcatg gttgccgtaa acgaaggcct cttgcccttc ggtggccagc cattcgatca
     2161 ggtcggcagc gagatcgggg tcgtccgcat cgctggtgat ccctgcacca ctgatgttga
     2221 catgtactcc acggccatcc tggttggccc agaagggaag tacgggaaag tcgggatctt
     2281 ccgcgagaag ccgtgccagg taatagtggt tggttattcc cacgtcgcag ccaccggccg
     2341 cgatcgtctc caaaatgagg ctgtcactgg tcatgatctg ggtctcattg gcgacccatc
     2401 cctggacgat ccgcagggcc tcctcgtaac cgagatggtg gatgaggctg gccaccagtg
     2461 actgcgtgta gacattggtg ctgtcacgca tgcacagacg ccccgaccac atcgggtctg
     2521 ccaggtcctc gtatgtagat agatcatcgg cgctgacgcg atctgggtgg tacatgatgg
     2581 tccgcactcg cttcgataga cccacccata gcttttcggg atgcacaagg ttgtcgggga
     2641 gactctcgat gacctcagac tccatcggct ggagtaggtc ctcgtcggcg gcgagttgca
     2701 ggttggcggc atcgacggtc atgaacacat ccgccagcgt gctctcaccc tcggtgcgga
     2761 ggcgctcacg gagctcggtg tccccgccgg ttaggaaatc gacctcgacc ccggtttctt
     2821 cggtgtactc gccgaatgcc tcttcgaggt cgtagtggcg ccctgtatag acgccgatga
     2881 cctgatcatt gtccagcgcg tcgaccaccc cgcaggcggc gagcgccacg ggaacgatga
     2941 gcagaaaagc gaatcctcct acggtcattc ggttcatcgg tccgtcctca ctacggaatc
     3001 gaatttgcac tacagcggta gtgtttatgt gttaggtgaa cctaacacac ggcgccgagg
     3061 cacgtgaact caggcgagta gttcgacgag gtcgaaccac atgaggatca gaagcaggat
     3121 gatcacaccg acgtggagaa cggtgatcgg cttgcgccac cggtgattcg ctctccagaa
     3181 ccgacggatg ctgaatacgt tggctgcaat cgccaccgcg ccgatgacta gtcccaggac
     3241 ggggcccacc cccggagcca aaccgaggaa gggcgtgaag aagggcagga ccacgtaggc
     3301 gagcacgcaa cgaatgcccg atatgagcat cgacattgtg aacaagcgtt gggtcgagtc
     3361 cgtggtcggc tggggctgga ctgtgatctc gcctgtcatc tgatccatcc cgaggaacgt
     3421 taaccgacag ttcgcgatgg cagaacccgc atcgccacac acgtcaaatt ggcccttgac
     3481 agctacatac cgagtatgca tactccgcaa ctagtaacgg agtatgtagg aaatgggcat
     3541 aagagaagga cttctcgcac ttctcgccga cggaccccgt cacggctacc aactcaagct
     3601 cgatctcgag atggccaccg gtgacgccgt aaccctcaat gtcggacagg tctacaccac
     3661 cctgcagcgt ttggagcgcg atgggttggt cgaggatggc ggcaccgacg tcgagggacg
     3721 cgccatctac cggatcacca agcccggcat tgcagcaatc agagaatggg cttctcgtcc
     3781 ggaggacctt gctgcggccg gacgcgacga gatctcgatc aaggtgcttc tcgcgctgtt
     3841 cactcccggt ctcgacccac gtcaggtcat cgaaactcag cgccgcgcca cgatggggat
     3901 g
//
LOCUS       DASUTA010000267         4547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_853680, whole genome shotgun sequence.
ACCESSION   DASUTA010000267 DASUTA010000000
VERSION     DASUTA010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853680"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1491
                     /locus_tag="VFZ80_05325"
     CDS             <1..1491
                     /locus_tag="VFZ80_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX6286885.1"
                     /translation="DVRVLERLPAPRPWAPALAIHARTMEALRGLGVADDLISRGLTK
                     VDLEVHVGDSVVSGTLGDLELPTTEYPFVFFAPQIEVEASLRERLASLGVNVEWASTF
                     RSFRTIDDEVVCRFDVRGQESLIHARYLAGCDGADSVVRRESGTAFRGRSYRESVVIA
                     DIDAAVALKDSTAHAFIGTNGILFLFPLPSGRWRLIGPGSGTATPETVTTMVETHARR
                     EIDVSAVDRFEVVRLQHRLADRYRRGRVFLAGDAAHVHSPAGAQGMNTGIQDAVNLGW
                     KLALVLKGAPESLLDTYDRERRRVAKQVVLWTGLAFALEVSKLAPFRWGRRWMALPAA
                     RLLLTRRRLVSVFGRVASGLDTSYREGALEVAGRLGRCRPGRRLPDHLIRAGETSRVH
                     HLITADRFHLLTFDEAVDVTRVRDLADAYGDLLRWHHVGSIEHEFAVSWALVRPDGYI
                     AAANRGADLEAAERYLLKWLGTPGVFGHVGIAEKSHASDRGSGRRP"
     gene            1515..2252
                     /locus_tag="VFZ80_05330"
     CDS             1515..2252
                     /locus_tag="VFZ80_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEX6286886.1"
                     /translation="MEEPNRSLADTIAELVESIVTDFDETEICFRLVDAAVEVVDADE
                     AGLLLMDRTGELGIAAATSASTHLVELLQVQAREGPCLDAFRTGEPVRTGPLVSDRAR
                     RTWPEFAPMAVDAGFHSVVAVPMRWRDTTLGSLNLFRTGPGDADDRDIVAARALADLT
                     TISIVQAQAMDGAHEVIDQLQTALNTRVTIEQAKGIVAARTRLGVDAAFEQIRRHARS
                     SNARLAEVAAKIVSGQLDADDLTGPDG"
     gene            complement(2253..2630)
                     /locus_tag="VFZ80_05335"
     CDS             complement(2253..2630)
                     /locus_tag="VFZ80_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6286887.1"
                     /translation="MAGSDLLVVEVDPIKDGAVMVRLKGEVDISSAHLLTSQFSSLAM
                     NTPSTVVLDATEVTFMDSTGLHALVEGKRALHDNGSRIFLVPSRQVRRVLELVFPDPL
                     FAFRLDSVEDALRQLDQEKAEPA"
     gene            complement(2736..>4547)
                     /locus_tag="VFZ80_05340"
     CDS             complement(2736..>4547)
                     /locus_tag="VFZ80_05340"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX6286888.1"
                     /translation="SSGYMTSQALGFVAANVAEPLVGASIVYAACEAVDLARRRHLIW
                     FIVGAVLIGPAVGAAIGAGADRLFGGDDFLTTFGQWWLGDALGVILVGGAILAWGSSL
                     DRRRITSVSGMILITGSIALTLIAVGLSDLPVVFSVLIGVVVAGVVFGVRAVTITSLV
                     IALTIAIVLIFDSEPVLVGMAPASALVLIKLQLGLFTLAGLLIAAESHERELASKEAV
                     RAALESEAWQRQRELDRDLAVALQRGLLPDRIPTRPGVDIAARYEAAGEAQEVGGDWY
                     DTIQLRDDRLALVVGDMVGHGMEAMISMGRTRTALSALAVHSESPSALLSELDQFVSQ
                     SAGSTYATIFYAVLDLQAGTVTYSSAGHPPALLVSPDGSLNWLDEAQTEPVYGRPSSR
                     IQASIAFDPGSCLILYSDGLIEQRGQSLAVGLDLLGRHGRELVDQTPEVICDELFRRV
                     AGPDRDDDVVVLVVKPGMDPDEFHEVFPARPDELRNLRTSLREWSEARGLSDGVSDDL
                     LISVGEAMANSVRHAYRDAVGGDVTVRITLVNEHLNVSVADRGKWRDPFDEEAFPGLG
                     TSIIDSVSQNLTIDKSGSGTLVTFRIPVSAPSRDVRA"
BASE COUNT          836 a         1425 c         1461 g          825 t
ORIGIN      
        1 gacgtccggg tcctcgagcg actccccgct ccaaggccat gggctccggc actggccatt
       61 catgcccgga caatggaggc acttcgcggg cttggtgtgg ctgacgacct cattagccgc
      121 ggattgacga aagtcgacct cgaggtccac gtcggcgatt ccgtcgtctc aggcaccctc
      181 ggtgatctcg aactgccgac gaccgagtac ccattcgtgt tcttcgcgcc ccaaatagag
      241 gttgaggcgt cgttgcggga gaggctggcg tcgttgggcg tgaacgttga gtgggccagc
      301 acgttccgga gctttcgcac catcgatgac gaagtcgtct gccgcttcga cgtcagggga
      361 caggagagcc tcatccacgc ccgttatctc gcgggttgtg acggcgccga cagcgtggtt
      421 cggcgtgaat ccggtacggc ttttcggggg cgaagctatc gagagtcggt agtgatcgcc
      481 gacatcgacg cagccgttgc cctgaaggat tcgaccgcac acgccttcat agggacgaac
      541 gggatcttgt tcctgtttcc acttccctct ggacgatggc gtctcatcgg tccgggatcg
      601 ggcactgcga cgcctgagac cgtgaccacg atggtcgaga cccatgctag gcgggagatc
      661 gatgtctcgg cagtggatag gttcgaggtc gtcaggctgc agcatcgtct ggcagaccgt
      721 tatcgacgag ggcgggtgtt cctggccggc gatgcggccc acgtgcacag cccggcaggt
      781 gcccaaggaa tgaacacggg catccaggat gcagtcaact tgggatggaa gctggccctc
      841 gtgctcaaag gtgcaccgga gtcacttctc gacacatacg atcgggagcg aagacgagtg
      901 gcgaagcagg tcgtcctttg gacaggtttg gcctttgccc tcgaggtgtc gaagctggcc
      961 ccgttccgtt gggggcgacg ttggatggcg ctgcctgccg ctcgactact cctaacgcga
     1021 cgccggctcg tttcggtgtt tggccgggtc gcctcggggc tcgacacgag ttaccgcgag
     1081 ggcgccctcg aggtagctgg tcgactcggt cgctgccgac ccgggagacg gctccctgat
     1141 cacttgatcc gagccgggga aacgtcccgg gtccaccacc tcatcacggc cgaccggttt
     1201 catctgctga ccttcgacga ggcggtcgat gtgacccgtg tccgggacct cgcggatgcc
     1261 tacggtgatc tgctgagatg gcatcacgtc ggttcgatcg agcacgagtt cgctgtttcc
     1321 tgggctctgg ttcggcccga cggctatatc gcggcggcaa accgcggcgc tgatctggaa
     1381 gctgccgagc gttacttgct gaagtggctc gggacccccg gagtcttcgg gcacgtgggg
     1441 atcgccgaga aaagccacgc atccgacaga ggcagcgggc gtcggcctta gctctgatct
     1501 ctatgctcgt tcccttggag gaaccgaacc ggagtctcgc cgacacgatt gccgagctgg
     1561 tcgagtcgat cgtcaccgac ttcgatgaaa ccgagatctg cttccggctc gtcgatgccg
     1621 cggtcgaagt cgtcgacgcc gacgaagcgg gtctgctgtt gatggacagg accggcgagt
     1681 tggggattgc ggcggcaacg agcgcttcga ctcacctcgt cgagctcctc caggtgcaag
     1741 ccagggaggg cccgtgcctg gacgcgtttc gcaccggtga gccggtccgg accgggccgc
     1801 tcgtgtctga ccgagcgcgc cgaacctggc cagagttcgc gccgatggcg gttgacgctg
     1861 gctttcactc ggttgttgcg gttccgatgc gatggcgaga cacaacgctc ggctcgctca
     1921 acctcttcag aaccgggccc ggcgacgccg atgatcgtga catcgtcgca gcccgggcac
     1981 tggccgacct gacgacgatc tccatcgttc aggcgcaggc gatggacggt gctcacgagg
     2041 tgatcgatca gctgcaaacg gcgctgaaca cgagggtgac catcgaacag gcgaagggga
     2101 tcgtcgccgc acgcacccga ctgggtgtgg acgctgcctt cgagcagatc cgacggcacg
     2161 cccgatcgag caacgcccgt ctcgctgagg tcgccgcgaa gatcgtgtcc ggtcagctgg
     2221 acgccgatga cttgaccgga cccgacggtt gatcaggccg gctcggcctt ttcctggtcg
     2281 agttggcgca gcgcgtcctc caccgagtcc aacctgaagg caaacaacgg gtcgggaaac
     2341 accagttcga gaacccgacg cacctgtctg gaggggacga ggaagatgcg tgagccattg
     2401 tcgtgcagcg cccttttccc ttcgaccaac gcgtggaggc cggtcgagtc catgaaggtc
     2461 acttcggtcg catcgagcac gaccgtggac ggtgtgttca tcgccaatga ggagaattga
     2521 ctggtcagga ggtgggctga ggagatgtcc acctctccct tgagcctgac catgaccgcg
     2581 ccgtccttga ttgggtccac ctctacaacg aggagatcgc tgcccgccat atccatgttg
     2641 ctatccctct gcgagtcaaa accgaaatgt catcttcggc gcccactccc agggtccaga
     2701 ggagttgccg tggtgaaaag tgtagacgac gcggttcacg ctcgcacatc tctagagggt
     2761 gctgagacag gtattcggaa ggtcaccagg gtccccgatc cgctcttgtc gatggtgaga
     2821 ttctggctca ccgagtcgat gatgctggtg ccgaggcccg ggaatgcttc ttcgtcgaac
     2881 gggtcgcgcc atttgcccct gtcggccacg gagacattca gatgttcgtt gaccagagtg
     2941 attcgaaccg tgacgtcacc tccgaccgcg tcccgatacg cgtgacggac tgagttggcc
     3001 atcgcttctc ccacggagat gagaaggtcg tcgctgacac cgtcggacag gcctcgggct
     3061 tcactccact cccgcagtga cgtcctcagg ttgcgcaact catcgggtcg tgccgggaac
     3121 acttcgtgaa actcgtccgg atccatcccg ggtttgacca ccagcaccac aacgtcgtcg
     3181 tccctgtcgg ggccggcgac acgacgaaac agctcgtcgc aaatcacctc cggggtctga
     3241 tcgaccagct cccggccatg tctcccgagg agatcgagac ccacggccag cgattggccc
     3301 ctctgttcga tgaggccatc cgaatagagg atgagacacg acccagggtc gaacgctatc
     3361 gaggcctgga ttcgactcga agggcggccg tagaccggct cggtctgcgc ctcgtcgagc
     3421 cagttgaggc ttccgtcggg agacaccagg agcgctggag ggtggcccgc cgacgaatac
     3481 gtgacggtgc cggcttggag gtcgaggacg gcgtagaaga tcgtggcgta ggtcgacccg
     3541 gccgattggc tcacgaactg gtccaattcg ctgagaaggg cggaggggct ctcgctgtgg
     3601 actgccaggg ccgagagggc ggttcgtgtc cgacccatcg agatcatggc ttccatgccg
     3661 tgaccgacca tgtcgccgac gaccagggca aggcggtcgt cccggagctg gatggtgtcg
     3721 taccagtcac cacccacttc ctgagcctcc ccggccgcct cgtacctggc cgcgatgtca
     3781 acgcccggtc tggtggggat cctgtcgggc agcaggccac gctgcagtgc cacggccagg
     3841 tcgcggtcca gctcacgctg tctctgccac gcctccgact ccagggcagc gcgaaccgcc
     3901 tccttgctgg ccagctctcg ctcgtgagac tccgccgcga tcaggagacc ggccaatgtg
     3961 aacaacccca actggagctt gatgaggacc agcgccgagg ccggtgccat ccccaccagg
     4021 acgggctcgc tatcgaagat gagcacgatt gcaatcgtca atgcgatgac caatgaagtg
     4081 atggtgaccg cccggacccc aaatacgacg cctgccacta cgacaccgat gagaacggag
     4141 aagacaaccg gcaggtcgct gagaccgacg gcaatcagag tgagagcgat cgaacccgtt
     4201 atgaggatca ttccgctgac ggacgtgatc cttcgtcgat cgagactcga cccccaagcg
     4261 aggattgctc caccaacgag aatgactccc agggcatctc ccagccacca ctgcccgaaa
     4321 gtggtcaaga agtcgtctcc gccgaagagc cgatcggcgc ctgcgccgat cgcggctccc
     4381 actgcgggcc cgatcaacac cgcgccgacg atgaaccaaa tgaggtggcg acgccgggcc
     4441 aggtccaccg cctcacatgc tgcgtagacg atcgaggcgc ccaccaaagg ctcggcgacg
     4501 ttggcggcaa cgaagccgag agcctgggac gtcatgtacc cactgct
//
LOCUS       DASUTA010000268         2047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1761741, whole genome shotgun sequence.
ACCESSION   DASUTA010000268 DASUTA010000000
VERSION     DASUTA010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1761741"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1866)
                     /locus_tag="VFZ80_05345"
     CDS             complement(<1..1866)
                     /locus_tag="VFZ80_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286889.1"
                     /translation="MTSRRLLIWLVAMVLVSAGCRSTTAPTPREHLSAGFRYSIYGPD
                     YNPGPEYWAGVGREMAADFPGAVPGTVWIVGRLDGSGTEMSFPGTSDDPLIRFTDKDL
                     NEEALTLFDQYGFDCWLQVEPGNASVEESIHLMLGRYGHHPCVVGVGVDVEWFRSVDE
                     PDGDAVTDQEARTWLAAAREYHPEYRLFLKHWLTEKMPPTVREGILFIDDSQIFPSLD
                     AMVEEFTRWGRAFAPAPVGFQYGYPSDRPWWRNHADPPAEIGRRILEAVPNAEALYWV
                     DFTAIEVFLPPPEIAAAHAARTSVAPDGAVIGVKIYEVPADLNRAFGRWEDLGITTAF
                     ASEAVAGDGAFRMMAEERGIEVFVIVPVFQDPEALEREPELVAITSRGAEAREDWVAF
                     VCPSREPYRTKKAEAVREMVRRLRPDGLSLDFIRHFVFWEKVHPATAHGDIPNACFCP
                     SCVASFSEATGIEIPAGLDGTIAIAEWILDNHGRAWTEWKIGLIDSMAWELVRSAREE
                     RPSIEINIHAVPWTRTDFGGAILRTAGQDFASLANYADYLSPMTYAHMVQRPPSWIHS
                     VVVRLAGESSAPILPSIQVKEAYRPGVELSDEEFEQHLREALKPPSRGVVFWSWEA"
     gene            complement(1863..>2047)
                     /locus_tag="VFZ80_05350"
     CDS             complement(1863..>2047)
                     /locus_tag="VFZ80_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286890.1"
                     /translation="EAARSATIGDTALVSCLTAVLREGEADAGPKWPDGTEAARQEPR
                     PPGRMNAVMFTKGRRT"
BASE COUNT          386 a          697 c          613 g          351 t
ORIGIN      
        1 ggcctcccac gaccagaaca ccacgcctcg cgaaggaggc ttcagcgctt cacgcaggtg
       61 ctgctcgaac tcctcgtcac tgagctcgac cccgggcctg tatgcctcct tcacctgaat
      121 gctcgggagg atcggcgcag acgattcccc ggccaggcgc acaacgaccg agtgaatcca
      181 actgggcggg cgctgcacca tgtgagcata ggtcatcggc gagaggtagt cggcgtagtt
      241 cgcgagcgat gcgaaatcct gccctgcggt tctgagaatg gcaccgccga agtcggtgcg
      301 ggtccacggg accgcgtgaa tgttgatctc gatcgacggc cgttcctccc gcgccgaacg
      361 aaccagctcc caggccatcg aatcgatcag accgatcttc cactctgtcc acgcccttcc
      421 gtgattgtcg agaatccact cggcgatcgc gatcgttcca tccagtcccg cggggatctc
      481 gatcccggtc gcctcgctga acgacgcgac acagctcgga cagaagcagg cgttcggaat
      541 atccccgtgg gcggtcgccg gatgaacttt ctcccagaag acgaaatgcc ggatgaaatc
      601 gaggctgagg ccgtccggac gcagccttcg gaccatctcc ctcaccgcct ccgccttttt
      661 ggtccgatag ggttcgcgag acgggcagac gaacgccacc cagtcctccc tcgcctcggc
      721 tccacgcgag gtgatggcga cgagctccgg ctcgcgttcc aatgcttcgg gatcctggaa
      781 cacgggtacg atcacgaaga cctcgatgcc acgctcctca gccatcatgc ggaacgcccc
      841 gtccccggct accgcctcgc tcgcgaaggc cgtggtgatc ccgagatcct cccatcgacc
      901 aaaggcccga ttcagatcgg cgggaacttc gtagatcttc acgccgatca ccgcgccatc
      961 cggtgcgaca ctcgtacggg ccgcgtgcgc ggcggcgatc tcgggtggcg ggagaaagac
     1021 ttcgatcgcc gtgaagtcga cccaatagag agcttccgca ttcggaactg cttcgaggat
     1081 tcgccgaccg atctcggcag gcggatcggc atgattgcgc caccaggggc ggtcgctcgg
     1141 gtaaccgtac tggaacccga ccggagccgg tgcgaaggcc cgtccccacc tcgtgaactc
     1201 ttcgaccatg gcgtcgagcg aaggaaagat ctggctgtcg tcgatgaaga gaatcccttc
     1261 ccgcaccgtc ggaggcatct tctcggtcaa ccagtgcttg aggaagagcc gatactcggg
     1321 gtggtactcc cgggccgcgg cgagccaggt gcgcgcctcc tggtcggtga cggcatcgcc
     1381 gtccggttcg tcgaccgaac ggaaccactc gacgtcgact ccgactccga cgacgcacgg
     1441 gtggtgccca tacctgccga gcatgagatg aatcgactct tcgaccgaag catttcccgg
     1501 ctcgacctgc aaccagcagt cgaatccgta ctggtcgaac aacgtcagcg cttcctcgtt
     1561 gaggtcttta tcggtaaacc ggatcagcgg atcgtcgctg gtgccgggaa aactcatttc
     1621 ggttccgctc ccatcgagcc ggccgacgat ccagaccgtg cccgggacgg caccgggaaa
     1681 gtccgccgcc atctcccgac cgaccccggc ccagtactcg ggcccggggt tgtagtcagg
     1741 accatagatc gagtagcgga accccgccga gagatgttcg cgcggagttg gagcagtcgt
     1801 cgaccgacag ccagcagaaa cgagaaccat cgcaacgagc cagatcagca accggcgaga
     1861 cgtcatgttc gtcttccctt cgtgaacatc accgcattca tacgaccggg agggcgaggc
     1921 tcctgccgag ccgcctccgt cccgtccggc catttcggcc cggcatcggc ctcgccctcc
     1981 ctgagaacag ccgtcaggca gctcacgagg gcggtgtcgc cgatggtcgc gctccgcgca
     2041 gcttctg
//
LOCUS       DASUTA010000269         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_337596, whole genome shotgun sequence.
ACCESSION   DASUTA010000269 DASUTA010000000
VERSION     DASUTA010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337596"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VFZ80_05355"
     CDS             complement(<1..1104)
                     /locus_tag="VFZ80_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEX6286891.1"
                     /translation="MAALTEQMHLAEALGELATTLESVEFGPATADLAVERDRLVGTI
                     RSFLVPRAIDPTAPLTVVLAGPTGSGKSTIANTLTGLDITPTGALRPTTTDPVVLTSP
                     DRVGNYESIGGVRCRVIAGHAPVLDTIVLVDTPDIDSTSTAHREVAETLIDNADIVLF
                     VTSALRYADEIPWQVLRRALSRGTPVINILNRVSPSTTGAIVDFRSRLAAAGLDDRIV
                     TMSEHHLAEGSQQVPSLAIRSLRRRLFSIVADRERFADDVFTRVLRATVDQIGDVARS
                     MAQLDDELSRLEADLSRDLAVRVSSIDFSGLGEGPGEGSSNRPLLPGTRRWKSGSGPT
                     VANGSILVRRLVAIVEGDIRNWLVDGHDALHSRH"
     gene            complement(1157..2032)
                     /locus_tag="VFZ80_05360"
     CDS             complement(1157..2032)
                     /locus_tag="VFZ80_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 1,2-dioxygenase"
                     /protein_id="HEX6286892.1"
                     /translation="MGEIVGVGLISHAPTVMFDEATRRELNDGKEISLVPGLVRLRSE
                     VLNRLRPDTIVIIDSHWESTVEHILSAHAHRSGHMTSSELPRGMAGIPYDYDGDPELA
                     QLAAKIGDELGTPTHATHDDYLPVYYATVNLVHYLHQDEAIVSVSVVQTGEPDDFLTM
                     GRALGEAVEQLERRVVVLASGGMSHTFWPLSQLTKHEASDPVHIITPEARAADEKRLE
                     WMQAGDHRRIVDTMDDYMPHNPEGGFGHYLMMLGAVGGADCTAPGVLFSDYENATGTG
                     QVHVWFERPSGGWTA"
BASE COUNT          372 a          656 c          722 g          377 t
ORIGIN      
        1 gtgacgtgag tggagggcgt cgtgcccgtc gacgagccag ttgcgtatgt caccctcgac
       61 gattgcgacc agacgccgca ccaggatcga cccgttggcg acggtcggcc ccgaccccga
      121 cttccatcgc cgcgtcccag gcagaagggg gcgattcgac gaaccttcgc ccgggccttc
      181 acccagaccg ctgaagtcga tcgagctcac ccggacggcc agatcgcggg agagatcggc
      241 ctccagcctg ctgagctcgt cgtcgagctg cgccatcgat cgggcgacat cgcctatctg
      301 gtcgacggtg gcgcggagca ctcgtgtgaa cacgtcgtcg gcgaatcgct cccgatcggc
      361 cacgatcgag aagagacgtc tgcgcagaga ccgaatagcg agggagggca cctgctggga
      421 gccctcggcg agatgatgct cggacatcgt gacgatccgg tcgtcgagac ccgcagccgc
      481 cagacgagac ctgaagtcga cgatggcccc tgtcgtcgag gggctcacac ggttcaggat
      541 gttgattact ggtgttcccc tggagagggc ccggcgcagc acctgccacg ggatctcatc
      601 ggcgtagcgg agcgcggagg tcacgaatag gacgatgtcg gcattgtcga tgagcgtctc
      661 agccacctct cgatgggcgg tggaggtcga atcgatgtcg ggtgtgtcga ccaggacgat
      721 ggtgtcgagg accggagcgt gaccggcgat cacacgacac cggacaccac cgatgctctc
      781 gtagttgccc actcgatccg ggctggtcaa aacgaccggg tcggtggtgg tcggcctcag
      841 ggcgccggtc ggggtgatgt cgagcccggt cagggtgttg gcgatggtcg acttccccga
      901 gccggtggga ccggcgagga caaccgtcaa aggtgccgtc gggtcgatgg ctctgggaac
      961 gaggaacgat cggatcgtgc cgacgagacg gtctctctcg acggccaggt ctgcggtcgc
     1021 cggcccgaac tcgaccgatt ccagcgtcgt ggcgagctcg ccgagtgcct cagcgagatg
     1081 catctgctct gtcaacgcgg ccacgactcg attctggcat ggctaccggc gcaggaccgg
     1141 tagtggaaac ggtgcttcag gccgtccagc cccctgaggg cctctcgaac cagacgtgga
     1201 cctggccggt gccggttgca ttctcgtagt cgctgaacag cacaccgggc gcggtgcagt
     1261 cggcaccgcc cacggctccg agcatcatca ggtagtgccc gaaaccccct tcggggttgt
     1321 gaggcatgta atcgtccatg gtgtcgacga tgcgacgatg gtcgccggcc tgcatccact
     1381 ccaaccgctt ctcgtcggcg gccctcgcct ccggcgtgat gatgtggacc ggatcggagg
     1441 cttcgtgctt ggtcagttgg gacagcggcc agaaggtgtg gctcatgcca cccgaggcca
     1501 ggacgacgac ccttctctcc agctgctcga ccgcttctcc aagggctcgc cccatggtga
     1561 ggaagtcatc gggctccccg gtctggacaa ccgagaccga gacgatcgcc tcgtcttggt
     1621 gcagatagtg gaccaagttg acggtggcgt aatagacggg gagatagtcg tcgtgagtgg
     1681 cgtgggtcgg ggtccccagc tcgtcgccga tcttggcggc gagctgggcc agttcgggat
     1741 ctccgtcgta gtcgtagggg atcccggcca tgccccgggg cagctcagag gaggtcatgt
     1801 gcccactgcg atgagcgtgt gcggagagga tgtgctcgac ggtgctctcc caatgggagt
     1861 cgatgatgac gatggtgtcg ggacggaggc gattcagcac ctcgctgcgg agacggacga
     1921 gaccggggac caggctgatc tccttcccgt cgttgagctc acgacgtgtg gcctcgtcga
     1981 acatgaccgt tggggcgtgg ctgatgagac cgacaccgac tatttcgccc atgtgtttca
     2041 ctttaacctc gtgcggtaac gacaccccgc gctccacatg ccatcaccca gatcagaagt
     2101 ctcagacgta gccgtatccc ccgacca
//
LOCUS       DASUTA010000270         7042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_596272, whole genome shotgun sequence.
ACCESSION   DASUTA010000270 DASUTA010000000
VERSION     DASUTA010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596272"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFZ80_05365"
     CDS             <1..208
                     /locus_tag="VFZ80_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX6286893.1"
                     /translation="FLFGPDRKLVYRGRMDEARPGNEVPVTGNELRAAIDAVLAGEPV
                     TGEQHASMGCSIKWAEGRQPAYFG"
     gene            348..857
                     /locus_tag="VFZ80_05370"
     CDS             348..857
                     /locus_tag="VFZ80_05370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX6286894.1"
                     /translation="MTKQYDNAPDLTIDPTKSYVATLDTNHGDIVIDLDPGRSPLTVN
                     NFVFLANDGYYDGVVFHRVIENFMIQGGDPTGTGRGGPGYKFRDELEGDGTYSRGTVA
                     MANAGPNTNGSQFFICHTDVGLPHSYTIFGQVRSGMDAVDSIATSDTDHSDRPNEAVV
                     INSVRITEE"
     gene            complement(917..1537)
                     /locus_tag="VFZ80_05375"
     CDS             complement(917..1537)
                     /locus_tag="VFZ80_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008738085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX6286895.1"
                     /translation="MRLTSTSFNDGDPIPERYAFAKPDPESRVTLAGNTNPHLQWSDI
                     PDGTLSFALICHDYDVPSKGDDVNQPDREVPEDLPRVDFFHWVLVDLPADKASIAEGE
                     FSEGVVAGGQEKRAHVGRHGLNDFTGWFAEDPDMAGNYYGYDGCAPPWNDSILHHYVF
                     TVYALDVDRLDVDGDFTGHDVREAMKGHILAEASIEGTYTQNPRLL"
     gene            complement(1555..2442)
                     /locus_tag="VFZ80_05380"
     CDS             complement(1555..2442)
                     /locus_tag="VFZ80_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6286896.1"
                     /translation="MKIGVQLPEVERPPSWDETRDIARTIEDCGFDSIWVGDHLLYRD
                     DRGTRGPFEAWTSLAGLAEVTSRVELGPLVASTSFHSPAMLAKMAATVDEISGGRLIL
                     GLGAGWNQVEYQAFGFPYDRRVSRFEEAFTIIRTLVREGAIDWDGEFYTLRDMELVPR
                     ARSDIKILVGSNGPRMLRATMAHADMWNSWFDGFGNQVEGLAELTSVVDDACEAVGRA
                     PGEVVHTVALYVQLPGGRGRQPGDDDRGTADPISGSRSEIADGLRSFQGAGISHVQIV
                     LDPIDAASVEELAEIVALI"
     gene            2465..4111
                     /locus_tag="VFZ80_05385"
     CDS             2465..4111
                     /locus_tag="VFZ80_05385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011602756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEX6286897.1"
                     /translation="MLDETSVLNHLQRRRHLQRMAAEGVDVLVIGGGVTGSGAALDAA
                     ARGLRVGLVEQRDYASGTSSRSSKMFHGGLRYLERFEFGLVREALRERNLMLNELCPH
                     LVTPVSFIYPLQHRFWERLYVGAGVLVYEVLARLASNPLPWHRHLGRRRLQSLAPALD
                     AAGGIRYWDALVDDARHTMTVARTAAAHGALIASSTRVVGLTKTPEGRIEAVRVVDLE
                     RGVEIAVATRAIVNATGVWTDDVETLAGDHQVDVKASKGVHFLVPRDRIESDTGILLR
                     TEVSVLFVIPWGEHWIVGTTDTPWSLHRAHPAASRSDIEYLLRWVNTAIRDPISTSDI
                     VGVYAGLRPLLQGETEETSRLSREHAVEDRGDGLITVAGGKYTTYRVMASDAVDATSS
                     YLDGIPDSPTAQIPLVGAAPFRDDRLHRRYGRLVDEIEELMAIDPDLARPVVSDAPYL
                     MAEIRYAVTHEGALHLDDVLTRRTRLSIETRHRGLEAARPAAELMGHVLGWDKATIDR
                     EIAHYEARVMAEIDSQSQPDDHTADAARMGAPDVRTGSSE"
     gene            complement(4112..5437)
                     /locus_tag="VFZ80_05390"
     CDS             complement(4112..5437)
                     /locus_tag="VFZ80_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX6286898.1"
                     /translation="MNRVTSLALRTCVFIALTGCTGEPGGGGVNIELDTTSTSAPVVS
                     TSTSVTTTSTTITTTTTLPSYHVTGRVTDEDGNPVARAAVSMGGDATTTGPDGWFAFD
                     ITDPAPMTISKPGWSSVEVDYSESVDFYEAAISPLTVRGLRVSPETAGDDTRFDRLLA
                     LARDTAVNSLVFDTKQEGGLVVYDTGVEDAHQIGAVTPHYDPVARIDRAHAEGLYTIT
                     RIVAFDDAFRAGAFGEERLIGGWLSPTSDGARSYVLSLAEEACQMGFDEIQFDYIRYP
                     SGSTATRTGQLDLPQSERVDAIAAFLAEARSLLNPMGCAVGGDVFGIVMSVDDDQGLG
                     QRPEELSAQLDVLSPMIYPSHYSDGWLGFDDPNDHPYDVTHDAISDALPRLAEGSTLR
                     PWLQAFWWTNAQIRSSIQAAEDQGVGWILWNVLSNFDVAALPTDAEVGS"
     gene            complement(5476..6816)
                     /locus_tag="VFZ80_05395"
     CDS             complement(5476..6816)
                     /locus_tag="VFZ80_05395"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX6286899.1"
                     /translation="MTGSDPTNLEAIARRVCEMAGDIEAQALVTRHRYGLTRFANSFI
                     HQHHGEDTTKVAVKVAVEGRVASASTTDDSDDGLSRLVDDATTAALLQPVDVHWPGVA
                     EPADTPDFEKTSAATIGAQPSDRADGVAAFIDAGTDLSAAGYLDTSFQESAVATTAGQ
                     SASGGATRAIIDGIHRGATSAGNGHQTSVDFSQLDAAAAGRRAADLARRGTDPIDLQP
                     GEYPVVLSPECVATIAIFLAGYGFGGQSYVDGQSFVELGERQFDESFHLIDDPLDSRV
                     VSLPFDADGTPKRTLPLVEAGVSKAIAHDRRTALRAGAESTGHAIPVSYFGPFPTSVV
                     VGAGTSSAQDLIGDVERGVYVSTFNYCRILDPRSQVVTGLTRNGTFLVESGEITRPLS
                     NLRFTQSFLNALGEGSILGIGNDQRYADSEFGPGLVIAPSMSLAAWRFTGGAQG"
     gene            complement(6813..>7042)
                     /locus_tag="VFZ80_05400"
     CDS             complement(6813..>7042)
                     /locus_tag="VFZ80_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007464914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX6286900.1"
                     /translation="GREIVDGKLGRLLRNPTYTGITPRFWASLDMLAGGDEWHPWGTP
                     NCGKGQPMQVGHTGHPAAPARFRNVRVGVSR"
BASE COUNT         1293 a         2271 c         2209 g         1269 t
ORIGIN      
        1 cttcttgttc ggtcccgacc gcaagctggt ctatcgcggt cgaatggacg aggcgcggcc
       61 cggcaacgag gtgcccgtga ccgggaacga gcttcgggcg gccatcgacg ccgtccttgc
      121 aggagagccc gtcaccggcg aacaacacgc ctcgatgggt tgttccatca agtgggccga
      181 aggcagacaa cctgcctact tcggttgaca acagacaatt ccgaacctgc ggtttgcgtg
      241 tccttccgac acgttggagt ctctgagaga ctcccgacgt ctcgacgttg ttcgagacgc
      301 cacgccgtat ccggcggaga tccgctcaac taccgagaag gaccccaatg acaaagcaat
      361 acgacaacgc ccccgacctg accatcgacc caaccaagtc ctatgtggcg acgctcgaca
      421 cgaatcacgg ggacatagtc atcgacctcg acccgggtcg atcgccgctg acggtgaaca
      481 acttcgtgtt cttggccaat gacggctact acgacggcgt cgtcttccat cgagtcatcg
      541 agaacttcat gatccaaggc ggcgacccca ccgggacggg acgtgggggg cctggctaca
      601 agttccgtga cgagctcgag ggtgacggca cctactcacg aggcacggtc gccatggcca
      661 acgccggccc caacaccaac ggcagccagt tcttcatctg tcacaccgac gtgggtttgc
      721 cccacagcta caccatcttt ggacaggtga ggtccggtat ggacgccgtg gactccatag
      781 ccacatcgga caccgaccac tccgaccgcc ccaacgaagc cgtcgtcatc aactcggtga
      841 ggatcaccga ggagtgatct tggcggtcgc ccgtgcacca agtctaggaa accgcttcat
      901 cttgatatag cgacgcctac aagagacggg ggttctgggt gtaggtccct tcgatggagg
      961 cctcggcgag gatgtggccc ttcatcgctt cgcggacgtc gtggcccgtg aagtccccgt
     1021 cgacgtccag ccgatccaca tccagggcgt acacggtgaa gacgtaatgg tggaggatcg
     1081 agtcgttcca tggaggggca cacccgtcgt agccgtaata gttcccagcc atgtccgggt
     1141 cctcggcgaa ccagcccgtg aagtcgttga ggccgtggcg gcccacgtgg gcacgctttt
     1201 cctggccgcc ggcaaccacg ccttcggaga actccccttc agctatcgag gccttgtccg
     1261 ctgggagatc gacgagcacc cagtggaaga agtcgactcg aggcagatcc tcggggacct
     1321 cgcgatcggg ctggttcacg tcgtctccct tgctagggac gtcgtagtca tgacagatca
     1381 gtgcaaaaga cagggttcca tcaggtatgt cagaccactg aagatgtggg ttggtattcc
     1441 cggcgagggt caccctcgac tccgggtccg gtttggcgaa tgcataacgc tcggggatcg
     1501 gatcgccgtc gttgaagctc gtcgaagtca gtctcatgag cccaacccta tagatcagat
     1561 gagcgccacg atctcggcca gctcttctac agaggcggca tcgatcgggt cgagcacgat
     1621 ctgaacatgt gagatccccg ctccctgaaa agaccggagt ccatcggcta tctcggagcg
     1681 gcttccggag atcgggtcag ctgtgccccg gtcatcgtca ccgggctggc gccccctgcc
     1741 cccggggagc tgcacgtaca gggccacggt gtgcaccact tcgccgggtg cgcggccgac
     1801 cgcttcgcag gcatcgtcga ccaccgaagt cagctccgcc agaccctcga cctggttgcc
     1861 gaaaccgtca aaccacgagt tccacatgtc ggcgtgggcc atggtcgctc tgagcatgcg
     1921 gggaccgttc gagccgacga ggatctttat gtccgaccga gccctcggca ccaactccat
     1981 gtcacgcaag gtgtagaact ccccatccca gtcgatggca ccttccctca caagggttcg
     2041 aatgatcgtg aatgcctcct cgaaacggct cacccgccgg tcatagggaa acccgaaggc
     2101 ctggtactcg acctggttcc atcctgcccc cagccccaga atgagtcgac caccactgat
     2161 ttcgtcgacg gtcgccgcca tcttggccag catcgccggt gagtgaaagg aggtggaggc
     2221 gaccagcggt ccgagctcga ctctggatgt gacctcagcc aggccggcga gactggtcca
     2281 ggcctcgaat ggccccctgg taccgcgatc gtcccgatag agcagatgat cgccaaccca
     2341 tatcgagtcg aacccgcagt cctcgatggt tcgggcgata tcccgggtct catcccagga
     2401 gggggggcgt tcgacctcgg ggagctgcac acctatcttc atcgggcaat cgtaaggagc
     2461 gccggtgctc gacgaaacat ccgtcctcaa ccatctacaa cgtcgccgcc atctgcagcg
     2521 aatggccgcc gaaggggtcg acgtgctcgt catcggaggc ggggtgaccg ggtccggtgc
     2581 tgccctcgac gccgcggctc gtggcctccg ggtgggtctc gtcgagcaga gggactacgc
     2641 gtcaggtacc tcgagccgtt cctcgaagat gttccacggt ggccttcgct atttggagcg
     2701 tttcgaattc ggcttggtgc gagaagcgtt gcgtgagcgc aatctgatgc tcaacgagct
     2761 ctgtccccac ctggtgactc cagtgtcctt catctacccg ttgcagcatc gattctggga
     2821 gaggctctac gtcggggccg gggtcctcgt ctacgaggtc ctcgctcgtc tcgcgtcgaa
     2881 ccctctcccc tggcatcgac atctggggag acgtcgactg cagagtctgg cgccggctct
     2941 cgatgcggcc ggaggcatcc gatactggga cgccctggtc gacgatgccc ggcacacgat
     3001 gaccgtcgcc cggaccgccg ctgctcatgg tgcgctgatc gccagcagca cgagggtggt
     3061 ggggctcacc aagactcccg aaggcaggat cgaggcggtg agggtcgtag atctcgaacg
     3121 cggggtggag atcgccgtcg ccacccgcgc catcgtcaac gccaccggcg tctggaccga
     3181 cgacgtcgag acactcgccg gggaccacca ggtcgacgtg aaggcttcga agggagtcca
     3241 cttcctcgtc ccacgcgatc ggatcgagtc ggacaccgga atcctcctcc gcaccgaggt
     3301 gagcgtgctt ttcgtcatcc cctgggggga gcactggatc gtcggcacga ccgatacgcc
     3361 ctggtcgctg caccgggctc atcccgcggc cagccgcagc gatatcgagt acttgctgag
     3421 gtgggtgaac accgcgattc gagatccgat ctccacatcg gacatcgttg gcgtgtatgc
     3481 cggacttcgt ccactcctcc agggcgagac cgaggaaaca tccaggctca gccgtgagca
     3541 tgcggtcgag gatcgcggtg atgggctgat caccgtcgcc ggagggaagt acaccaccta
     3601 ccgcgtcatg gcctccgacg ccgtcgatgc caccagttcc tatctggatg gcatcccgga
     3661 ttcgccaaca gctcagattc cgctcgtggg tgcggctccg ttcagagacg atcggcttca
     3721 ccgtcgctat ggcagactgg tcgacgagat cgaggaactg atggcgatcg accctgatct
     3781 cgcccgtccc gtggtctctg atgctcccta cctgatggcg gagattcgct atgcggtgac
     3841 ccacgaaggg gcactccatc tcgacgacgt cttgaccagg aggacccgtc tgtcgatcga
     3901 gacccgccat cgaggactcg aggcggctcg cccggctgca gagttgatgg gtcacgtgtt
     3961 gggctgggac aaggccacga tcgatcgtga gatcgcccac tacgaagcga gggtgatggc
     4021 cgagatcgac tcccagtccc aacccgacga ccacaccgcc gacgcagcgc gaatgggcgc
     4081 tcccgacgtc cggaccggga gcagcgaatg atcaggatcc gacctcggcg tcggtgggga
     4141 gagcggcaac atcgaagttg ctgaggacgt tccagagaat ccaacccacg ccctggtcct
     4201 ccgcagcctg gatcgagctc cgtatctgcg cattcgtcca ccagaaagcc tgcaaccacg
     4261 gtcgcagcgt cgagccctcg gccaaacgtg gcagcgcatc gctgattgcg tcgtgcgtca
     4321 cgtcatatgg gtggtcgttg gggtcatcga acccgagcca tccatcggaa tagtgactcg
     4381 gatagatcat cggactgagc acgtcgagct gcgccgagag ctcctccggg cgctggccga
     4441 ggccttgatc gtcatccacc gacatcacga tcccgaaaac atccccaccc acggcacacc
     4501 ccatcgggtt gagaagcgaa cgggcctcgg cgagaaacgc ggcaattgcg tcgacgcgct
     4561 cggactgggg caagtcgagc tggccggtcc gggtcgcggt cgaccctgat ggatatctga
     4621 tgtagtcgaa ctggatctcg tcgaagccca tttggcacgc ctcctcggcg agggaaagga
     4681 cataggaccg tgctccatcg gaggtggggc tcagccagcc cccgatcaga cgctcctcgc
     4741 cgaaggcccc ggcgcggaag gcgtcgtcga aggcgacgat gcgggtgatc gtgtagaggc
     4801 cctcggcatg ggcccgatcg atgcgtgcca cggggtcgta gtgcggagtg acggcaccga
     4861 tctggtgagc gtcttccaca cccgtgtcgt agacgaccag tcccccctcc tgcttggtgt
     4921 cgaacacgag cgagttcact gcggtgtccc tcgccaacgc caggagtcga tcgaaccttg
     4981 tgtcgtcacc ggccgtctcc gggctgaccc gcagacctct cacggtgagc gggctgatcg
     5041 cagcttcgta gaagtcgaca ctctcggagt agtccacctc aacgctgctc cagcccggct
     5101 tcgagatagt catcggggcc gggtcggtga tgtcgaaggc gaaccatcca tcaggccctg
     5161 tggtggtggc gtcgccgccc atcgagacgg cggcgcgggc gaccgggttt ccgtcttcat
     5221 cggtgactct ccccgtcacg tggtagctgg gaagcgtggt ggtcgtggtg atggtcgtgc
     5281 tcgttgtcgt gaccgaggtc gacgtcgaga ccaccggggc tgaggtggat gtcgtgtcga
     5341 gctcgatgtt caccccgccg ccgccgggct cgcctgtgca gcctgtgagc gcgatgaaga
     5401 cacatgtgcg cagagcaagc gaggtgactc ggttcacagt ggcgatgcta agaccggtcg
     5461 cgcactcaca ctcgcttaac cctgtgcacc accggtgaac ctccaggcgg cgagactcat
     5521 ggagggcgca atgaccaaac caggaccgaa ctcgctgtcg gcgtatcgct gatcgttgcc
     5581 gatccccaag atcgaaccct caccgagagc attgaggaag gactgtgtga accgaagatt
     5641 gctcaacggt ctggtgattt caccgctctc gacgagaaac gttccgttcc gggtcagccc
     5701 ggtgactacc tgacttctcg ggtcgaggat gcggcagtag ttgaaggtgg acacatacac
     5761 gccgcgctcg acatccccga tcagatcctg cgccgaggaa gttcctgcac cgacgacaac
     5821 cgacgtcggg aaggggccga agtacgacac cggaatggca tgtcccgtgg actccgctcc
     5881 tgcccgcagg gccgtccggc gatcgtgcgc gattgccttg ctgacaccgg cctcgaccag
     5941 cggcaacgtt cgcttcggcg tcccgtcggc gtcgaacgga aggctgacga ctctggagtc
     6001 gagtggatca tcgatcagat ggaacgactc gtcgaattgg cgctcgccga gttccacgaa
     6061 cgactgccca tctacatagg actgcccccc aaagccatag cccgccagga atatggcgat
     6121 cgtggcgaca cactcgggtg acagcaccac cgggtactcc cctggctgga ggtctatcgg
     6181 gtcggtcccg cggcgggcga gatccgcggc ccggcgtccc gcggccgcag catccaactg
     6241 agagaagtcg accgatgtct ggtggccgtt cccggccgac gttgcccccc gatgtatccc
     6301 gtcgatgatg gccctcgtcg cgccgccgga cgccgactgc ccagcggtag tggccaccgc
     6361 agactcctga aacgaggtgt cgagatatcc ggcggcgctg agatcagtcc ctgcgtcgat
     6421 gaaggcggcg accccgtctg cccgatcgga cggctgagcg ccgatggtgg ctgcggaggt
     6481 cttctcgaag tcgggggtgt ccgcgggctc tgccacgccg ggccagtgga catcgactgg
     6541 ttggagaagg gcagcggtcg tggcgtcgtc taccagccgg ctcaggccgt cgtcggagtc
     6601 gtcggtggtg ctcgccgagg ccactcgacc ctccaccgcc accttgactg caactttcgt
     6661 ggtgtcctcg ccgtgatgct ggtgaatgaa ggaattggcg aatcgggtca atccgtacct
     6721 gtgtctggtc acgagcgcct gggcctcgat gtcgcccgcc atttcacaga cccgccgggc
     6781 gatggcctcc aggttcgtcg ggtccgaccc ggtcatcggg agaccccgac tcgaacattg
     6841 cggaatcgtg ctggggcggc ggggtgcccg gtgtgtccga cctgcatcgg ctggcccttc
     6901 ccgcaattgg gcgtgcccca tggatgccac tcatcaccac cggcaagcat gtccaaggac
     6961 gcccagaacc gtggggtgat ccctgtgtat gtcgggttcc tgaggagacg tccgagcttg
     7021 ccgtccacga tttcccgccc ca
//
LOCUS       DASUTA010000271         4064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1892052, whole genome shotgun sequence.
ACCESSION   DASUTA010000271 DASUTA010000000
VERSION     DASUTA010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1892052"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VFZ80_05405"
     CDS             complement(<1..715)
                     /locus_tag="VFZ80_05405"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6286901.1"
                     /translation="MTEATAPTPVRRVSRITPVSVFLWFLRIVTLLVILYGSIQSISS
                     GRLTAAQWRDLVVFGVSTGSVYALIALGYTMVYGVLRFINFAHGEVFMTGAMSAFLVA
                     DGLASTPLWASNPVASLLIVLLASMATSTAVAVIVERVAYRPLRGAPRLIPLITSIGA
                     SFTIQYVVRGLVGPSTKAFPRVEQLRGTWEIFGLVILKSQVVVILGALLMMAGLYIFV
                     ERTRTGRSMRAVAEDPETAA"
     gene            complement(783..2036)
                     /locus_tag="VFZ80_05410"
     CDS             complement(783..2036)
                     /locus_tag="VFZ80_05410"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6286902.1"
                     /translation="MKRKKALLALFVGFALFITACGDGGGEEPEVGELGAIEVADGEP
                     IKIASLQAISGDVASLGTDQVRGVELAIADKGEIAGHSIELAFNEDDLCNSEGGQTGA
                     TRIVADPDVVAVIGTSCSGAGVPASAVVSDANRVLISGSNTSPVLTSDLAGTAGPEYH
                     EGYFRTAHNDAIQGAAAATFVFDELGLTKVATVNDGDPYTQGLTGAFEAAYEELGGEI
                     VVSTAVTADQTDMRPVLTEIAAAGAELIFFPIFQPAGDFLAEQALEVAGLEDTVLMGA
                     DGLLSDTYVVLPQSKGMYFSGPQTPTTPAYDDFVAAYEEAYGEPPIQSFHAHAYDAAM
                     MVFAAIENIAEESDGNLVIDLQALRDELHSITYDGLTGSLSCDEFGDCAAPAIDIVQN
                     TDDTADIGAVRSNVQATYTREDLGL"
     gene            2161..3252
                     /locus_tag="VFZ80_05415"
     CDS             2161..3252
                     /locus_tag="VFZ80_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286903.1"
                     /translation="MAENVGVVEDGVTTEAPRRRSNPGWILAATGFVMGLGLGVLVVN
                     PPETSAPPVSEAPTNGAPTPTTTTPIEDLGIASSIPYFPDTLIAVAQTTGSTLDHILW
                     PLRGEMITRSMSGGSDVALDSTSRFIALSEPLPGDEGALLSMGRFHRLGLVAGDVTSY
                     AWHDSVSGDLAYTTESDGMWRLFVVRSSFTPRLVTESMEFGGTLVGFGDWGWAIQTSA
                     DELVLLTPDGDFKAVESGVGYASHRDGWIFVVDERPKLVSAGGGVVRVDTNLDVGEIT
                     VAAFSPDGSRVAIGGERGLAVLYLRSGVAEPFGGSSVSNVAWSSDSRFVMSSAGSGVV
                     VHDIDTIDRYQILQGRRVMAVGTAPLGDS"
     gene            3262..4005
                     /locus_tag="VFZ80_05420"
     CDS             3262..4005
                     /locus_tag="VFZ80_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEX6286904.1"
                     /translation="MAGSGPTEELAPVDSYLSTAEGHVLEIADGGIFLDRTVFYARGG
                     GQPGDVGTITWDGGSVEVVDTIRRDGKPFHLVTEGSALPDPGTAVEGIIDWERRYLTM
                     RTHTALHALSGVVFRDYGAKVTGGNMTPGEARMDFELDGISQEFGQEIERILNAELVK
                     GYPTEIITMAREIALQDPDLIRTKANLIPDFVKEIRVVDIKGLDRQADGGTHVNSTLE
                     VGRIEVVKTESKGKSNKRMRIRVTDPEAT"
BASE COUNT          775 a         1270 c         1256 g          763 t
ORIGIN      
        1 gggctgccgt ctccgggtcc tcggccacgg ctcgcatgga tcgccctgtg cgggtccgtt
       61 ccacgaagat gtagagcccg gccatcatca gcagggcgcc gagtatgacc acgacctgtg
      121 acttcaggat cacgaggccg aagatttccc aggtgccccg cagctgttcc actcggggga
      181 aggccttcgt gcttggaccg accaggccgc ggaccacata ctggatggtg aaggacgcac
      241 cgatggaggt gatgagcgga atgagccttg gcgcgcctcg cagcggccga taagcgacgc
      301 gttcgacgat gaccgcaaca gcggtcgacg tggccatgct cgccaatagc acgataagca
      361 acgaggccac agggttcgaa gcccaaaggg gagtggaggc cagaccatcg gcgacgagga
      421 acgccgacat ggcaccggtc atgaacacct cgccatgggc gaagttgatg aagcggagca
      481 cgccgtaaac catggtgtac ccaagcgcga tcagggcgta caccgacccc gtcgatacgc
      541 cgaagaccac gaggtcgcgc cattgcgctg ctgtgagtct cccagacgag atgctttgga
      601 tcgacccgta gaggatgact agcagggtga cgatccgcag gaaccacaga aaaacggaaa
      661 cgggtgtgat ccgcgagacc cggcgcacgg gcgtcggagc ggtggcttcg gtcatgctct
      721 gcccatctgt tgtgagaccg gcaccacttc gagaagtggt gccggtctcg tgcgttatag
      781 ggtcagagac ccaagtcctc tcttgtgtag gtcgcctgga cgtttgagcg caccgcaccg
      841 atgtccgccg tgtcgtccgt gttctggacg atgtcgatgg cgggcgcagc gcagtcaccg
      901 aactcgtcgc aagacagaga tccggtgaga ccgtcgtagg tgatgctgtg caattcgtcg
      961 cgcagtgcct ggaggtcgat cacgagattg ccatccgact cctcagcgat gttctcgatc
     1021 gccgcgaaca ccatcatcgc cgcgtcgtat gcgtgtgcat ggaacgactg aatcgggggc
     1081 tcgccgtagg cctcctcgta ggcagcgacg aagtcgtcgt aggcaggggt cgtcggtgtc
     1141 tgcggtccgg agaagtacat ccccttcgac tgcgggagca cgacataggt gtccgaaagg
     1201 agaccgtcgg cgcccatgag caccgtgtcc tccaacccgg ccacttccag ggcctgttcg
     1261 gcgaggaagt cgccggccgg ctggaagatc gggaagaaga tgagctcggc gcccgccgcg
     1321 gcgatctcgg tgagcaccgg tcgcatatcc gtctggtcgg ccgtcacggc tgtgctgacc
     1381 actatctccc ctccgagctc ttcgtaagca gcctcgaagg cgccagtcag accttgggtg
     1441 taagggtcac cgtcgttcac tgtggcgacc ttggtcagac ccagctcgtc gaacacgaac
     1501 gtggccgcgg ccgcaccctg gatggcgtcg ttgtgcgccg tccggaaata gccctcgtga
     1561 tattccgggc ctgccgttcc tgccaggtca gacgtgagga ccggagaagt gttcgatccg
     1621 gagatcagaa cgcgatttgc atcggagacc accgcagatg ccggcacgcc ggcaccagaa
     1681 cagctggttc cgatgactgc aaccacgtcg ggatccgcca cgatgcgcgt tgcgcctgtc
     1741 tggccaccct cagagttgca taggtcgtct tcgttgaacg caagttcgat cgaatgacca
     1801 gcgatttctc ccttgtccgc gatggctagc tcgacccctc gcacctggtc ggtgccgagg
     1861 ctcgctacgt cgccgcttat cgcttgcagc gaggcgatct tgataggttc gccatctgcg
     1921 acctcgatcg cccccagctc tcctacttcg ggctcttcgc cccctccgtc accacatgct
     1981 gtgatgaaca gggcaaatcc cacgaagaga gccagaagtg ctttcttcct tttcatgggt
     2041 tccttcctcc aatttgaaag tgacaggatc tgggtcctgt cggtggtcga ctttagacct
     2101 tgggacgttt attggcatct gagaccacac aaattcgttt ctcacgtacc gttctgagcg
     2161 atggcggaga atgtcggagt cgtcgaggac ggtgtcacca ccgaggctcc ccgccgccgg
     2221 tccaacccag gctggatact cgccgcgacc gggttcgtca tgggcctcgg cctgggcgta
     2281 ctggtcgtca atcctcccga gacctcggcc ccacccgtat cagaggcacc cacaaacgga
     2341 gcgccgacac caactaccac cactcccatc gaggacctcg gcatcgcgtc ctccatcccc
     2401 tacttccctg acacgctcat agccgtcgcc cagaccaccg ggagcactct cgatcacatc
     2461 ctctggccac tcagagggga gatgatcacg cgatcgatgt caggtggatc agacgtcgcc
     2521 ctcgatagca cctcccgttt catcgcactc tccgagcccc tgcccggcga tgagggcgcc
     2581 ctgctctcca tggggaggtt ccaccggctc ggccttgtcg ccggcgatgt cacgagttat
     2641 gcatggcacg actcggtatc gggcgacctc gcttacacca ccgagagcga cgggatgtgg
     2701 cggctcttcg tcgtccgatc cagcttcact ccccggttgg tcaccgagag catggaattc
     2761 ggggggacat tggtgggttt cggagactgg ggttgggcga tacagacctc tgcagacgag
     2821 ctggtattgc tcactccaga cggtgacttc aaagccgtcg agtcgggtgt cggatacgcc
     2881 tcacatcgtg acggctggat cttcgtcgtc gatgagcgac ccaagctcgt gtctgcggga
     2941 ggcggcgtgg tgcgtgtgga caccaacctg gatgtgggtg aaatcacagt cgctgcgttc
     3001 tcgcccgacg gcagccgtgt ggcaatcgga ggcgagcgtg ggctggcggt tctctatctg
     3061 cgatccgggg tcgccgaacc gttcggcggc tcgtcggtct caaatgtggc gtggtcctcc
     3121 gactccaggt tcgtgatgtc ctcagccggc tccggcgtgg ttgtacatga catcgacacc
     3181 atcgacaggt atcagatact gcagggccgg cgggtcatgg ccgtcggaac tgcacctcta
     3241 ggtgactcct agagtcggcg catggccgga tcaggaccaa ccgaggagct cgcacccgtc
     3301 gactcctatc tatctacagc cgagggtcac gttctcgaga tcgccgatgg tggcatcttc
     3361 ctcgatcgaa ccgtcttcta tgccaggggc ggcggacaac ccggcgacgt gggcaccatc
     3421 acctgggacg gaggctctgt ggaggtcgtc gacaccattc gcagagacgg aaagcccttc
     3481 cacctagtga ccgagggctc cgcgctaccc gaccctggca cagccgtgga aggcatcatc
     3541 gactgggagc gtcgctatct caccatgcgc actcacaccg cgttgcacgc cctctcgggt
     3601 gtcgtcttcc gcgactacgg cgccaaggtg accgggggca acatgacacc gggggaggcg
     3661 cgaatggact tcgagctcga tggcatctcc caggagttcg gccaggagat cgagcggata
     3721 ctcaacgccg agctggtgaa ggggtatccc acagagatca tcaccatggc acgggagatc
     3781 gcgcttcaag accctgatct catccggacc aaggccaatc tgatcccgga tttcgtcaag
     3841 gagattcgag tggtcgacat caagggcctg gatcgtcagg ccgatggcgg aacccacgtg
     3901 aactcgactc tcgaagtcgg gcggatcgag gtcgtcaaga ccgagtcgaa aggcaagtcg
     3961 aacaaacgga tgaggatcag agtcaccgac ccggaagcca cttgactcga acccggggtt
     4021 cctcgcgcca tatccggcgc aaatccgctc atcctcgcta cgtt
//
LOCUS       DASUTA010000272         3125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1243940, whole genome shotgun sequence.
ACCESSION   DASUTA010000272 DASUTA010000000
VERSION     DASUTA010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243940"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1186
                     /locus_tag="VFZ80_05425"
     CDS             155..1186
                     /locus_tag="VFZ80_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286905.1"
                     /translation="MKPGSSRRLEVRGVTKQFEGRPVLKGVDLTVEKGTSVTLLGPSG
                     CGKTTLLRVIAGLERPEQGTVTLGDRTLASADAFVPPEKRRIGMVFQDWALFPHLDVA
                     SNVAYGLSKDIPTRQRAVADALDMVGLSGFERRMPSTLSGGEQQRGAIARAIAPKPDA
                     LLLDEPFSNLDAALRATVRSDTRALLRELEMTTLFVTHDQGEAFVLGDEVAVMRDGVI
                     HQQAPADQLYSSPIDPWVAAFVGEANLLLADANGDTAHTILGDIPLAEQAFGRRTVLL
                     RPEQIYLSPGSSGKVTSVEFYGHDCSYRVEVNGTELIVRSIAAPVFSRGDDVSLSYEG
                     PGAVAYPAV"
     gene            complement(1248..1895)
                     /locus_tag="VFZ80_05430"
     CDS             complement(1248..1895)
                     /locus_tag="VFZ80_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX6286906.1"
                     /translation="MAEYGRYREYAEAIWEIEELDIPVIQARIADWLGVSRASVSEMV
                     HRMKEDGLVTVGDEIRLTDDGRHLAAVIVRRHRLAERFLTDILKLPWDKVHAEAEIWE
                     TTISDQVEEAMWAALEDPQTCPHGNPIPGAGYRPPKMKPISAMEPGETRPLERISEEL
                     ELDDEMLGFLYSSGFHPKADIELVGRDPKGAYTVSVGGKRVGVGEFAADRLFVAA"
     gene            complement(1941..2252)
                     /locus_tag="VFZ80_05435"
     CDS             complement(1941..2252)
                     /locus_tag="VFZ80_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286907.1"
                     /translation="MAAPFVANSAVDGREVAPPFRSHTSTCLKCQARHAAMSRTAREL
                     AAMHDQRFAAPADLEWRVLSSLEGDLEVARTWRKPIALSAAVLSMAAAIIIWRLRPRT
                     S"
     gene            complement(2267..2800)
                     /locus_tag="VFZ80_05440"
     CDS             complement(2267..2800)
                     /locus_tag="VFZ80_05440"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6286908.1"
                     /translation="MDLISRAQAGDRAAFAVLVREHQDEVYTLARRLVGDPHLASDVA
                     QEALIRAWRALPKFRGDARFSTWIYRITVNTAWTHKARAGRHQAASIEEHTQIPSTVE
                     TEHPEFAGEIVELRGRLRHALDRLPDAQREVVVLKDIYGWSHAEIAHSMGITVTAAKV
                     RLHRARARLARDLEESA"
     gene            complement(2838..>3125)
                     /locus_tag="VFZ80_05445"
     CDS             complement(2838..>3125)
                     /locus_tag="VFZ80_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX6286909.1"
                     /translation="TRDWNPVHWDHEAAVTAGFPGVVVHGLLQAGWALRAAASGTTGP
                     QPFASARFRFRNPLLPARPARVSTEAKNGAVDVVIAEDDVEYLTATVTLSE"
BASE COUNT          544 a         1010 c          985 g          586 t
ORIGIN      
        1 aaatcgtgac tcgttcgtcg tccggtgacg ccgaccaggc tgccaggtcg taatgctgag
       61 ccgagccctc agcctgctcg atgatgtcag aaagggcgat gtaaggcttc ccgtacattt
      121 acggtctggg gttcctgttg ataccattgc ccacgtgaaa cccgggtcct cccggcgtct
      181 cgaggtacga ggcgtcacca agcaattcga aggtcgacct gtgctaaagg gcgtggacct
      241 gacggtggaa aagggaacgt cggtcaccct gctcggtccg agcggatgcg gtaagaccac
      301 cctgctccgc gtcatcgccg gtctggagcg acctgaacag ggcacggtga cgttgggcga
      361 tcgaacgttg gccagcgctg acgcgttcgt ccctccggag aagcggcgta tcggcatggt
      421 cttccaggat tgggctctct tcccgcacct ggatgtggcg tccaacgtcg cctacggact
      481 ttccaaggac attcccaccc gtcaacgcgc cgtagctgac gccctggaca tggtgggcct
      541 gtcggggttc gagaggcgga tgccgtcgac cctctcagga ggcgagcagc agcgcggtgc
      601 catcgcccgg gcgatcgccc ccaagcccga tgcactctta ctcgacgagc cattctccaa
      661 cctcgatgcc gccttgcgcg caacagtgcg atccgacaca cgggccctac ttcgcgagtt
      721 ggagatgacg acattgttcg tcacccacga tcagggagag gcgttcgtcc tgggagatga
      781 ggtcgccgtc atgagagacg gtgtaatcca tcagcaggcc cccgccgatc agctctactc
      841 cagcccgatc gatccttggg ttgccgcctt cgttggcgaa gccaacctcc tcctcgctga
      901 tgcgaacggg gacacagctc ataccatcct cggtgacatt ccccttgcag agcaggcatt
      961 cggccgtcgg actgttcttc tgcgacccga gcagatctat ctctcacccg gttcgtccgg
     1021 gaaggtgacg agcgttgagt tctacggaca cgactgctca taccgcgtcg aggtcaatgg
     1081 gacggagttg atcgtccgct cgatcgccgc accggtcttc tcccggggtg acgatgtatc
     1141 gctctcatac gagggccccg gcgcggtggc ctacccggcc gtataggcga ctcgagaacc
     1201 gacgacaccc atcggtgcgg ttcgactgac gagaagagac gggagcctca cgcggcgacg
     1261 aagagccggt cggcggcgaa ctctccgact ccgacacgct tgcccccaac cgacactgtg
     1321 taagcaccct tggggtcacg tcccaccaac tcgatgtcgg ctttgggatg aaaccccgag
     1381 ctgtagagga atccgagcat ctcgtcatcg agctccagct cctctgagat gcgctcgagc
     1441 ggcctggtct caccgggctc catggcgctt atcggcttca tcttgggtgg ccgatagccg
     1501 gcaccgggga tcggattgcc gtgagggcag gtctgtggat cctcgagagc ggcccacatg
     1561 gcttcttcga cctggtccga gatggtggtc tcccagatct cggcctcggc gtggaccttg
     1621 tcccatggga gcttcaggat gtcggtgagg aagcgctcgg cgagccggtg tctccgcacg
     1681 atgactgcgg cgaggtgccg accgtcgtcg gtgagtctga tctcgtcgcc cacggtcacg
     1741 agaccgtctt ccttcattcg atgaaccatc tcgctgacac tcgcccggct cacgccgagc
     1801 cagtctgcga ttcgggcctg gatcaccggt atatcgagct cctcgatctc ccagatggcc
     1861 tctgcgtact cgcgataacg tccgtattca gccaaggtga cggggatcgt agtgccgccc
     1921 ctgctcgacc cgctccggcg tcaggatgtg cgaggtcgga gtcgccagat gatgatggcc
     1981 gcggccatcg acaggaccgc cgccgacaga gcaatcggct ttcgccaggt ccgtgccacc
     2041 tcgaggtcgc cctccaatga ggacaggacc ctccactcca gatcggccgg agcagcaaac
     2101 cgttgatcgt gcatggcggc cagctctcgg gcggtccgtg acatcgcggc atgtcgtgcc
     2161 tggcacttga gacaggtgga ggtgtgagac ctgaagggtg gggccacctc tcgcccatcg
     2221 accgcgctgt tggcgacgaa aggggcagcc atctcgcaga cgacgctcat gccgactcct
     2281 ccagatcacg tgcgagccgg gcacgggccc gatgaaggcg gacctttgcc gcggtcacgg
     2341 ttatccccat ggagtgggcg atctcggcat gggaccaccc gtagatgtcc ttgaggacga
     2401 cgacctcccg ctgcgcgtcc ggaagacggt cgagggcgtg acggagacga cctctgagct
     2461 cgacgatctc gccggcgaac tccggatgct cggtctcgac cgtggagggg atctgggtgt
     2521 gctcctcgat ggatgcggct tggtgccgac cggcgcgcgc tttgtgcgtc cacgccgtgt
     2581 tcaccgtgat gcggtagatc catgtggaga accgggcatc gcccctgaac ttggggaggg
     2641 cgcgccaggc gcggatgagc gcttcctgag cgacatctga agccaaatgg gggtcgccaa
     2701 ccagtcttcg tgccaaggtg tagacctcgt cttggtgctc tctcaccagc accgcgaagg
     2761 cggcacggtc accggcctgg gctctgctga tgagatccaa ctctctttga tcttcgaccg
     2821 gggccgaccc gctcgggtta ctcactcagc gtcaccgttg ccgtcagata ctcgacgtcg
     2881 tcctcggcga tcaccacatc gacagctccg ttcttcgctt cggtggagac cctcgccgga
     2941 cgggccggga gcagcgggtt tcgaaaccgg aatctggcgc ttgcgaaagg ctggggcccg
     3001 gtcgtcccgg acgcggcagc tcgcaacgcc catcccgcct ggagaagccc gtggacgacg
     3061 acaccaggaa accccgcagt cacagcggcc tcgtgatccc aatgcaccgg attccaatcg
     3121 cgggt
//
LOCUS       DASUTA010000273         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_338357, whole genome shotgun sequence.
ACCESSION   DASUTA010000273 DASUTA010000000
VERSION     DASUTA010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338357"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2083)
                     /gene="secA"
                     /locus_tag="VFZ80_05450"
     CDS             complement(<1..>2083)
                     /gene="secA"
                     /locus_tag="VFZ80_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX6286910.1"
                     /translation="EFESLSDQELKSKTAEFKERVANGAELDDVKVEAFATVREAAKR
                     VLGQRHYDVQVVGAAALHRGMVAEMKTGEGKTLASTMPAYLNALSGKGVHMVTVNDYL
                     ASRDAEWMGAVHTFLGLSIGLIQNNMPTADRHPAYSADITYGTNNEFGFDYLRDNMAM
                     TAENRVQRGHPYAIVDEVDSILIDEARTPLIISGRLSDSAKWYRDFAKIARRLQPEVH
                     YEVDEKKRQVLVTEEGVSRVEGILGVENLYDHAAVDFVHHLETSLKAETLYHKDVEYL
                     IDHGEIKIVDEFTGRVLEGRRYSEGLHQAIEAKEGVAIKEENQTLATITLQNYFRLYE
                     KLAGMTGTAETEAAELASIYNLEVATIPTNEPVARLDQADLVYKTEAGKFDALIEDVK
                     SRHERKQPVLLGTVSIEKSERLSKALENVGVKHEVLNAKHHAREADIIAQAGRPGAVT
                     VATNMAGRGVDIMLGGNPEGMAKTELKQRGVEPLDPSYEEMERKLSEEYADQIRPDKE
                     VVIEAGGLYVVGTERHESRRIDNQLRGRSGRQGDPGESRFYLSLEDDLMRRFQGEWVA
                     GIMERLRMPEDQPIEAKMVSKSIERAQRQVESQNFEIRKNVLKYDEVMNTQRENIYAW
                     RNSILEGTASEDLIEDWVADVISGVVETQFEGVAKSEWDWEALRRELEQFYPTSVDAS
                     GFDGSFDIDDV"
BASE COUNT          314 a          683 c          629 g          457 t
ORIGIN      
        1 atgacgtcgt cgatatcgaa tgagccgtcg aatccggatg cgtcgacact ggtgggatag
       61 aactgctcga gctcccgccg aagcgcctcc caatcccatt cgctcttggc cacaccctcg
      121 aattgcgtct ccaccacgcc ggatatcacg tctgccaccc agtcctcgat caggtcctcg
      181 gaggcggttc cctcgaggat cgagttgcgc caggcgtaga tgttctcgcg ctgggtgttc
      241 atcacctcgt cgtacttcaa gacgttcttc cggatctcga agttctgcga ctccacttgg
      301 cgctgagccc gctcgatcga cttgctcacc atcttcgcct cgatgggctg gtcctcgggc
      361 atccgcagcc gttccatgat gcccgcgacc cattcgccct ggaagcgccg catcaggtcg
      421 tcttcgagag acagatagaa ccggctctcc ccgggatctc cctgacgacc cgaccggccg
      481 cggagctggt tgtcgatgcg ccgggactcg tgtcgctcgg tgccgacgac gtacaggccg
      541 cccgcctcga tcacgacttc cttgtccggc cggatctggt cggcgtactc ctcggagagc
      601 ttgcgctcca tctcctcgta ggacggatcc aagggctcca cgccgcgctg cttcaactcg
      661 gtcttggcca tgccctcggg gttccccccg agcatgatgt cgacaccacg ccccgccata
      721 ttggtggcga cggtgactgc accgggacgt cccgcctggg cgatgatgtc ggcctcacgg
      781 gcgtggtgtt tggcgttgag gacctcgtgt ttgaccccga cgttctctag cgccttggag
      841 agtcgctccg acttctctat cgagaccgtg ccgagcagca cgggctgttt cctctcgtgt
      901 ctcgacttga cgtcttcgat cagggcgtcg aacttgccgg cttcggtctt gtagacgaga
      961 tcggcctggt cgagacgggc caccggctcg ttggtgggga tggtcgccac ttccaggttg
     1021 tagatcgagg ccaactccgc ggcctcggtc tcagccgtgc cggtcatgcc ggcgagcttc
     1081 tcgtagagcc tgaagtagtt ctgaagggtg atggtggcga gcgtctggtt ctcctccttg
     1141 atcgccaccc cctccttcgc ctcgatcgcc tggtggaggc cttcggagta gcgacggccc
     1201 tcgagcaccc ggccggtgaa ctcgtcgacg atcttgatct ccccatgatc gatcaggtac
     1261 tcgacgtcct tgtggtagag ggtctccgcc ttgagagagg tctcgaggtg gtggacgaag
     1321 tccacagcgg catggtcgta gaggttctcg acccccagga tcccttcgac ccgggacact
     1381 ccctcctctg tgaccagcac ctgtcgcttc ttctcgtcga cctcgtagtg gacctcgggt
     1441 tgcagtcttc gcgcgatctt ggcgaagtcc cggtaccact tggcactgtc gctcaggcgt
     1501 ccagagatga tcaggggtgt cctcgcctcg tcgatcagga tcgagtccac ctcgtccacg
     1561 atggcgtagg gatggccccg ctgcaccctg ttctcggccg tcatggccat gttgtcgcgc
     1621 agatagtcga agccgaactc gttgttcgtg ccataggtga tatcggccga gtaggccggg
     1681 tggcggtcgg ccgtcggcat gttgttctgg atgaggccga tgctgagccc gaggaacgtg
     1741 tgcaccgcgc ccatccactc ggcatcacga ctggccagat agtcgttgac ggtcaccatg
     1801 tgaacgccct tgccggacag tgcgttgaga taggcgggca tggtggaggc gagggtctta
     1861 ccctctcccg tcttcatctc ggcgaccatc ccgcgatgca gagcggcggc accaaccacc
     1921 tggacgtcgt agtgacgctg gccgagcaca cgcttggcgg cctcccgcac cgtcgcaaac
     1981 gcctcgacct tgacgtcgtc gagctctgcg ccgttggcta ctcgctcctt gaactcggct
     2041 gtcttggact tgagctcctg gtcgctgaga ctctcgaatt cgg
//
LOCUS       DASUTA010000274         4011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_854750, whole genome shotgun sequence.
ACCESSION   DASUTA010000274 DASUTA010000000
VERSION     DASUTA010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854750"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..1795
                     /gene="malQ"
                     /locus_tag="VFZ80_05455"
     CDS             266..1795
                     /gene="malQ"
                     /locus_tag="VFZ80_05455"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX6286911.1"
                     /translation="MASPDLHGSTAAQRSSGLLLHPSSLPGSYGIGDLGPSAHDFVRL
                     LADAGQRYWQMLPLVPIGPGNSPYLSISTFASNSLLISPERLHDRGLLDAASLAGAEL
                     GATTRVDYGLVFETKLRLLHEIAAEFSDRADPDQKSRFAAFSDEHGPLWLDDFALFTV
                     LSDRIRVPWTDWPEGLARRHRPAIERAEAEFGDEIEIEKVLQFLFFEQWSDLCAAAFE
                     NGVRIVGDMPLYVGHDSADVWAHPESFLLDADGHPLVVSGVPPDYFSETGQRWGTPIY
                     DWDEMAGGDFEWWRARMRRAIDLFDLVRFDHFRGVAGYWAIPAENETAVEGTWEEGPG
                     MRLLDAIEDELGGLPLLAEDLGVITEDVITLRDRFGLPGMRVAQFGFDDAPDTEIHHP
                     ATYPENVWGYTGTHDNDTTLGWFWAQGPRIVSQLEAKRRRLHEVTGGDVVWGLIELVA
                     GSRARTSIFPVQDILELGSDARMNTPGTAIGNWEWRLLPGQLDDHAMAKLAEVTRGAG
                     R"
     gene            2056..2955
                     /locus_tag="VFZ80_05460"
     CDS             2056..2955
                     /locus_tag="VFZ80_05460"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6286912.1"
                     /translation="MPTGFNPLDRVIGGGLRTRNLTLVGGAPGAGKTITTLQWARNLA
                     QNGHRAVFACYEHDEATLLGRLLHLELGGLPTSERLSERGRSARATLTRLDAGEISLS
                     EAMAEAPILREPQARLRDYADRLWLVRASGASTTLEELGRLVETDTDALFVDYIHKVP
                     VADDRADEKERITIVAQGLKELAMSADVAVVAVAAANVEGLAAPRLRIRHLRGSSAIA
                     YEADVIVMLNDKYDIVSRSQMTYDLSNAESFKGRLIFSVEKNREGDDMVDIEFAKQFD
                     YLRVDPEGTHVKERLIDNRLFTE"
     gene            complement(3002..3220)
                     /locus_tag="VFZ80_05465"
     CDS             complement(3002..3220)
                     /locus_tag="VFZ80_05465"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6286913.1"
                     /translation="MSDTGRIHDQLELYTTGALTETETIVFDEHLQSCRDCQARAPDL
                     FEVAAALIPDSPASDQTWDRIVAAIERR"
     gene            complement(3217..3777)
                     /locus_tag="VFZ80_05470"
     CDS             complement(3217..3777)
                     /locus_tag="VFZ80_05470"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6286914.1"
                     /translation="MGTAEEHTSHDDAALVARIADGDRNALETVFRLYGGAVKSMALR
                     VLRNETLAEDTVQDVFVRFWESPKRFDPNRGTLRTFLVTIAHRRAVDAVRSEQARFNR
                     EEKVPEDVPGSVDDEVWSRVVSDQVRAAVESLADGEREAIELAYFGHLTYVDVAKKLG
                     LPEGTVKSRIRSGMRRLSLALAEVSS"
BASE COUNT          728 a         1302 c         1260 g          721 t
ORIGIN      
        1 ccgcagcgct ggcacgtgct cggcgcgagg agtcgaggtc gattcctcta cgaccggcct
       61 catcggtaga cgctcaccgg ctgtcctgcc cttcgtcgca cagctcgaag ccgaaccagc
      121 cagagtcatc gaggaccgcc ggccagaggc gccatggata cagggttggc tgaccccgag
      181 tgtcaggctc cgggtgcgaa cccaactgga aacgactccc gatcacggcg agatcgtcac
      241 tgccggaagc tgcttgcatg acgtcgtggc cagcccggac ttgcatggat cgactgcagc
      301 gcaacgctcg agcggcttgc ttctccaccc gagctcgctc ccgggcagct acgggatcgg
      361 tgacctcgga ccatccgccc acgacttcgt cagactgttg gccgatgccg ggcagcgtta
      421 ttggcagatg ctgccactgg tcccgatcgg gcccggcaat tcgccctatc tatcgatctc
      481 gactttcgcc agcaacagcc ttctgatcag cccggagcgt cttcacgacc gagggctgct
      541 cgacgctgct tctctggccg gggccgagct cggggccacc accagggtgg actacggcct
      601 cgtctttgag accaaactcc gcctgctcca cgagatcgcc gccgaattct ccgaccgagc
      661 cgatccggat cagaagtccc gattcgctgc cttcagcgac gagcatggcc cgctttggct
      721 cgacgacttc gccctattca cggtgctcag cgatcggatc agggtccctt ggaccgactg
      781 gccagagggt ctggcccgac gacatcgccc cgcaatcgaa cgagccgagg ccgagttcgg
      841 ggatgagatc gaaatcgaga aggtgctgca attcctcttc ttcgagcagt ggagtgacct
      901 ctgcgcggcg gcattcgaga acggcgttcg aatcgtcggc gacatgcccc tgtacgtggg
      961 ccacgactcc gccgatgtct gggcgcatcc cgaatcgttc ctgctcgacg ccgacggaca
     1021 ccctctggtc gtctctgggg ttcctcccga ctacttctcg gagacagggc agaggtgggg
     1081 cacaccgatc tacgactggg acgagatggc aggaggcgac ttcgaatggt ggcgcgcccg
     1141 gatgagacgc gccatcgatc tgttcgacct cgtccggttc gatcacttcc gtggtgtcgc
     1201 cggttactgg gcgataccgg cagagaacga gacagccgtg gaaggaacct gggaggaggg
     1261 gcccgggatg agactcctgg acgcgatcga agacgagctg ggagggcttc ccttgctcgc
     1321 cgaggacctc ggtgtcatca ccgaggatgt gatcacattg cgcgacaggt tcgggttgcc
     1381 cggtatgagg gtggcccagt tcggattcga cgacgcccct gacacagaaa tccaccaccc
     1441 cgcgacctac cccgagaacg tctggggcta caccggtacc cacgacaatg acacgactct
     1501 cggatggttc tgggcccaag gcccacggat cgtgagccaa ctcgaagcga aacgccgtcg
     1561 actccatgag gtgacgggtg gggacgtcgt ctggggtctc atcgagttgg tggctggttc
     1621 ccgggcccgg acctcgatct tcccggtcca ggacatcctg gaattgggta gcgatgcccg
     1681 gatgaacaca ccgggaaccg cgatcggcaa ttgggagtgg cgtctccttc cggggcagct
     1741 cgacgatcat gcgatggcca agctcgccga ggtgacccgt ggcgccggcc ggtaggaata
     1801 gcgcgccggc gaccggcgat gaaggcccag agtctcgagg gcgagaccag gagatcagga
     1861 ccatcaacgg ctgcgtcctc cgggagaaga atcactcggc gacttctcga ctgctcttca
     1921 tccgggtaac gtctcgcctc gttgttgacg gtttctcgct ggggggcggc aatggtcttg
     1981 aaggattcaa tcgagcgtaa gtcgatctcg tctgtcctgg acgaggccgg taatggggcc
     2041 aggttccgtc ggacgatgcc gaccggattc aaccctctcg atcgcgtcat cggtgggggg
     2101 cttcgcaccc ggaacctgac cctcgtgggc ggtgctcctg gagcggggaa gaccatcacg
     2161 actctgcagt gggcccgcaa cctggcacaa aacgggcacc gggcagtatt cgcttgttac
     2221 gagcacgacg aggccacgct tctgggccgt ctgctccacc tcgagctggg cggtttgccg
     2281 acgtctgaac ggctcagcga gcgcggccgt tccgcccggg caacgctcac caggctggac
     2341 gctggggaga tcagcctgag tgaggcgatg gcggaggcac cgatactgcg cgagccgcaa
     2401 gccaggctgc gcgactatgc cgatcgactc tggctggtcc gggcctctgg tgctagcacc
     2461 actctcgagg agctggggag gctggtcgag accgataccg acgcgctttt cgtcgattac
     2521 atccataagg tcccggtcgc cgacgatcgc gccgatgaga aggagaggat caccatcgtc
     2581 gcccaagggc tgaaggagct cgccatgagc gccgatgtcg cggtggtggc agtggcggcc
     2641 gccaatgtag agggtctggc agcaccccgg cttcgcatcc gccacttgcg cggatcttcc
     2701 gcgatcgcct acgaagccga cgtcatcgtc atgctgaacg acaagtacga catcgtgtcg
     2761 aggtcacaga tgacctacga cctcagcaac gccgagtcgt tcaagggccg tttgatcttc
     2821 tcggtcgaga agaaccgcga aggtgacgac atggtcgaca tcgagttcgc caagcagttc
     2881 gactacttgc gggtcgaccc cgaaggcacc cacgtcaaag agcgcctcat cgacaaccgg
     2941 ctcttcaccg aatgacaatc caccgacaca aagcttccga cgtcggctag ctgccagatg
     3001 ttcagcgccg ctcgatggcc gccacgattc gatcccacgt ctggtccgag gcgggtgaat
     3061 cggggatcag ggccgccgcc acctcgaaca gatccggggc ccgggcctgg caatctcgac
     3121 aactttgcag atgctcgtcg aagacgatcg tctctgtctc ggtgagggca cccgtcgtat
     3181 acagctcgag ctgatcgtga atccggcccg tatcgctcat gacgacacct ccgccagagc
     3241 caggctcaga cggcgcatcc cgctacggat ccggcttttc accgtgcctt cgggcaatcc
     3301 cagcttcttg gccacatcga cgtaggtgag atgcccgaag taggcgagct cgatcgcctc
     3361 ccgctccccg tcggccagtg actcgaccgc ggcccgaacc tggtcggaga ccacccggct
     3421 ccagacttcg tcgtccaccg agccggggac gtcctcgggc accttctcct cccggttgaa
     3481 cctcgcctgc tcggagcgca ccgcgtctac cgccctgcgg tgagcgatgg tcaccaggaa
     3541 ggtccgcagg gtgccgcggt tgggatcgaa gcgcttcggc gactcccaga acctgacgaa
     3601 gacgtcctgc accgtgtcct cggccagcgt ctcgttgcgg agcacccgca gcgccatcga
     3661 cttcaccgca cctccataga gacgaaacac cgtttcgaga gcgttgcggt ctccgtccgc
     3721 gattcgcgcg acgagggccg cgtcgtcatg gctggtgtgc tcctctgcgg ttcccacagc
     3781 ggcagacgat acgtgctgat cgccttgtga agccgatgga aggcgggtgg gcctcgcact
     3841 agcctcctca tcgccgcttc aggagaggca cagttgactg ccccggactt tccagaaatc
     3901 gagttggcgc tgctctccag cgcaatgtcc ggagacgccg gaaagctgta ccggatcgcc
     3961 tccaacctga tggatcaggg ggtttccttc gactcggtcc tcttcgacta c
//
LOCUS       DASUTA010000275         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_209226, whole genome shotgun sequence.
ACCESSION   DASUTA010000275 DASUTA010000000
VERSION     DASUTA010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209226"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFZ80_05475"
     CDS             complement(<1..281)
                     /locus_tag="VFZ80_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX6286915.1"
                     /translation="MDPMQLPTEPEILTTTVGSYSPIDWLITSPSEQALLDATAAVIH
                     TQRRAGIDLPTDGELYRFDANHAETNGMIEYFVSRMGGVRSEIGRSDNAA"
     gene            596..748
                     /locus_tag="VFZ80_05480"
     CDS             596..748
                     /locus_tag="VFZ80_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286916.1"
                     /translation="MDDHNDALAAEKRGRHERTPPVFLSSFSRSESYVIQPSGGWFAT
                     SNEPVD"
     gene            complement(937..1314)
                     /locus_tag="VFZ80_05485"
     CDS             complement(937..1314)
                     /locus_tag="VFZ80_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286917.1"
                     /translation="MTQEESPAQRVSSGGGSPGLLVIGAGLMIVNWILLGLLAGEWNP
                     GALYIALALLVLASAYNIGGVSLSPSTNRVIGLFMGLAALVIVVADLRFGSFPDDALR
                     VVAYIVFVASAALMFIGARQLTD"
     gene            complement(1482..>2204)
                     /locus_tag="VFZ80_05490"
     CDS             complement(1482..>2204)
                     /locus_tag="VFZ80_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6286918.1"
                     /translation="SRTSTAARLRQSKHLEGEKAAGRTLRTATRASQRAAESVAGTTM
                     PKTVGGEIRFDFEPAPRRLLASHLGPLTAGGTTIIDHVDIGVERADRIWLRGRNGVGK
                     TTLLRALVADADLPVDKLLYLEQEMTPEQVAEVMRETATLEPKQRGEVLALVALLGVD
                     PRVLLASRSPSPGEARKLAMALGLGRGSWLVALDEPTNHLDLPSIERLQAALASYPGA
                     LIVVSHDDEFARALRMEPFDLS"
BASE COUNT          401 a          669 c          729 g          405 t
ORIGIN      
        1 gcggcattgt cgctgcgtcc tatctctgat cgaacgccgc ccatgcggga gacgaaatat
       61 tcgatcatcc cgttggtctc ggcatggttg gcgtcgaatc ggtagagctc tccgtcggtg
      121 ggcaggtcga tgccggctcg ccgttgcgtg tggatcacag ccgcggtggc atcgagtagg
      181 gcctgctcgg acggcgacgt gatcagccag tcgatgggcg aatacgaccc caccgtggtc
      241 gtgaggatct ccggttctgt gggcagctgc attgggtcca tgcgactgga ccgtatcgca
      301 gtcggccggc gccatctcac cattgctcga ggtgcctttg tgtagccggc cttggtgagg
      361 ttggatggaa cgattggcga ggctcgatgg tcgaagcagc agggaggaga cccggcccgc
      421 cgcaccggca tcagctcgcg aaacttcagc ctcacacacg gtcaaggtcg gagggtgaac
      481 ctgccgatac cggatcggga tgatcgggcg caggagaaag acagaggcgg agtggcgtcg
      541 ggaggatcga acgggccgtc acgaaggggg tagggggttc aagcctccgg tcacgatgga
      601 cgaccacaat gacgcactgg cagcggagaa gcggggtcgg cacgagcgaa cccctcctgt
      661 gtttctcagc tcgttcagcc ggtccgagtc ctatgtgatc cagcccagtg gcggttggtt
      721 cgcgacgtcc aacgaacccg tggactaggc cgacgcgacc gatcgattga cacgcgtcac
      781 gaactcgtgg ccgtgtaatc agatcgagat atcctgccag agagtccttt gggctgacga
      841 tcgtggccta tccaacacca tctatctctg atccgttggc gccgtgctgg atggcggttc
      901 gtggatgaga gatttcgccg ccatcgatcg agtggttcag tctgtcagct gtctcgctcc
      961 aatgaacatc agagcggcgg aagccacgaa cacgatgtag gcaacgaccc tcagggcgtc
     1021 atcgggaaag gacccgaagc gcaggtcggc caccacgatc acgagggcgg ccagccccat
     1081 gaacagaccg atgacccgat tcgtgctcgg gctcagggag acacctccga tgttgtacgc
     1141 ggaggccagg accaagagcg cgagggcgat gtagagagca cccgggttcc actccccggc
     1201 cagcagcccg agcaggatcc aattgacgat catcaatccc gctccgatca ccaacagacc
     1261 tggtgatccg ccaccagagc tcacccgctg ggctgggctt tcctcttgcg tcatcctcga
     1321 ccctcctttt ggtcaccaag agtgtaagga gcaagaggcc agcgaagtcg tctgaaacgg
     1381 cacctccgtc cccgaatctg tcggagctgt gcctcatgac cgatccgaac ctgcccggtg
     1441 cgcatgctgg tcgtggcgac tcgagcgttt gccgggctcg gtcacgacag gtcgaagggc
     1501 tccatgcgca atgctcgggc gaactcatcg tcgtgggaca cgacgatgag agcacctggg
     1561 taggaggcaa gggcagcttg gagtcgttcg atggaaggga ggtcgaggtg attggtcggc
     1621 tcgtcgagcg ctaccaacca ggagccccgg ccgagaccga gggccatggc cagcttgcgc
     1681 gcctctcccg gtgaaggact gcgtgaggcg aggagcacgc gggggtcgac tccgaggagt
     1741 gcaaccaagg cgagcacctc accccgctgt ttgggctcga gagtcgccgt ctctctcatc
     1801 acttcggcga cctgttcggg ggtcatctct tgttctagat agagcaactt gtcgacgggt
     1861 aggtcggcgt cggcgaccag ggcacgcagc agggtggtct tgcccacccc gttgcgtcct
     1921 ctcagccaaa tccgatcggc tctctccact ccgatgtcga cgtggtcgat gatcgttgtc
     1981 ccgcccgcag tcagggggcc gagatgggag gcgagaagcc gccgcggcgc aggctcgaag
     2041 tcgaacctga tctcaccgcc caccgtcttc ggcatggttg tcccggcgac actctccgcc
     2101 gctcgctggc tggctcgggt tgcggtgcgg agtgtcctgc cggccgcctt ctccccttcg
     2161 aggtgcttcg actggcgcag ccgagcggcc gtcgatgtcc ttga
//
LOCUS       DASUTA010000276         2949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1504303, whole genome shotgun sequence.
ACCESSION   DASUTA010000276 DASUTA010000000
VERSION     DASUTA010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504303"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VFZ80_05495"
     CDS             <1..858
                     /locus_tag="VFZ80_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX6286919.1"
                     /translation="LEALGDRVMGETVTGVRIASPSVLRTYDPPYNAPVGKEVNSLHR
                     VGKRIVLGLEADLYVVIHLMVAGRLRWDSPGKSIPRKVGLAAFDFEDGALILTEQGTK
                     KRAGIWIMSGWGEVLGLDRGGIEPLEVSSEEFAAALRSENRTLKRALTDPRIFSGIGN
                     AYSDEILWSAQLSPVKRTSQMTDDEVARLHASVVTSLDEWTQRLRDEVGDGWPTKVTA
                     FRDDMAVHGKFGQPCPRCGSRVQRIVYSDNETNYCATCQTEGKLLADRSLSRLLKEDW
                     PKTLEELEG"
     gene            complement(869..1273)
                     /locus_tag="VFZ80_05500"
     CDS             complement(869..1273)
                     /locus_tag="VFZ80_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286920.1"
                     /translation="MDAEPESHDTESVLAGAGDFTSGEGLVAFAGLVIIGVWVIFSII
                     VTEYFISWLMLLLAVAAAVLPRLDRYKVEKAQSLPVLMKVIGYAIAIIGVFALVEDLR
                     FGEFEDIGDILGGLATYAACVMAFLGARQIDI"
     gene            complement(1375..2802)
                     /locus_tag="VFZ80_05505"
     CDS             complement(1375..2802)
                     /locus_tag="VFZ80_05505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6286921.1"
                     /translation="MVADIFAWLGFATILYFIAMQVYLLGLTVLSGFVLRRNHHLRRF
                     NRGTAMLSSTNAPPVSIVIAAYNEEAGIVDAVRSISIAKYPRFEIIVANDGSQDATLE
                     TLIGAFRLEKVRIPYRPDLESEPIRAIYRGRSGVDITVIDKENGGRADALNAGINAAR
                     FPYVFCTDADVILEPDCLVHAMRRVVDDRERTVGVGGNVRPLNGSRIELGHLISANVP
                     TRLIPRMQVLEYVRTFLASRPAWSRINALPLVSGAFGIWKRSTLVDVGGFARGHMGED
                     LDLTMRVHRLHMDRGDDYRIVYEPSAVVWTEVPDTLRVLRRQRIRWHRGLMTAISDFR
                     AMTFNPRYGKLGMVTWAGFFLFEYLAPIVEFVGWFVIPVAWLLGSLDTTSLIWMLVIA
                     FGAGLANSLTALLLDESYGYFNSPADTSRLVAMALLENFGPRQMTVVWRIRALMGGSA
                     TRHWGNMERRGVANLAKPTRTARTG"
BASE COUNT          568 a          916 c          932 g          533 t
ORIGIN      
        1 ctggaggccc tgggcgatcg agtgatgggg gagaccgtta ccggggtgcg aattgcgtcg
       61 ccttcggtgt tgcgcacgta cgaccctccc tacaacgctc ccgttggcaa ggaggtcaac
      121 tcgttgcatc gggtggggaa gcggatcgtg ctcgggctcg aagccgatct ctatgtcgtc
      181 atccatctga tggtggcggg ccgtctgcga tgggattccc ccggcaagtc gattcccagg
      241 aaggtcgggc tggcagcctt cgacttcgaa gacggggctc tcatcctcac cgagcaaggc
      301 accaagaaga gggcggggat ctggatcatg tcgggatggg gcgaagttct cggcctcgat
      361 cgaggtggta tcgagccgct cgaggtgagc agtgaagagt tcgccgctgc gttgcggtcc
      421 gagaatcgga ctctcaagcg ggcattgacc gatccccgca tcttctccgg catcgggaat
      481 gcctactccg acgagatcct ctggtcggcg cagctgtctc ctgtcaagcg cacctcgcag
      541 atgaccgacg acgaggtcgc caggctccat gcatcggtcg tcacatcgct ggacgagtgg
      601 acccagcgtc tccgggacga agtcggcgac ggttggccca ccaaggtgac ggcatttcgg
      661 gacgacatgg cggtccatgg caagttcgga caaccttgcc cgagatgtgg ctcgagggtg
      721 cagcgcatcg tctactcgga caacgagacc aactactgcg ccacctgtca gaccgagggg
      781 aagttattgg ccgatcgttc gctttcgcga ctgctcaaag aagactggcc caagacgctc
      841 gaagagctcg agggctagtg aggagcgatc agatgtctat ctggcgagct cccaggaacg
      901 ccatcacaca ggctgcgtag gtggcgagcc cacccaggat gtccccgatg tcctcgaact
      961 cgccgaacct caggtcctcg acgagtgcga agacgccgat gatcgcgatt gcatagccga
     1021 tgaccttcat caagaccggc aacgactggg ccttctccac cttgtacctg tccagcctgg
     1081 gcagcaccgc cgcggccaca gccagcaaca gcatcaacca cgagatgaag tattcggtca
     1141 cgatgatcga gaagatcacc caaacgccga tgatcaccag tccggcgaat gcgaccaggc
     1201 cctcaccact ggtgaagtct cctgcgccgg cgagaaccga ctcggtgtcg tggctctccg
     1261 gctcagcgtc catgggtgga gctgctgcag ggggctctgt cggcggctgc attgggtccc
     1321 gggggtgctc gtcggtcatc tcatctcctc gccgtgtgtt ccgaccggcg gactctatcc
     1381 ggttcgagca gtccttgtcg gtttcgccag gttggccacg ccccgcctct ccatgttccc
     1441 ccaatgtcga gtggcgctac cacccatgag agccctgatc ctccatacca cagtcatctg
     1501 ccggggaccg aagttctcga ggagggccat ggccaccagc cgcgaggtgt cagccgggct
     1561 gttgaagtag ccgtaggact cgtcgaggag aagagcggtg agagagttgg ccaacccggc
     1621 tccaaatgca atgacgagca tccagatcag cgaagtcgtg tccagagagc cgagaagcca
     1681 ggcgacaggt atgacgaacc agcccacgaa ctcgacgatc ggagccaggt attcgaagag
     1741 gaagaacccg gcccatgtca ccattcccaa cttgccatag cgcgggttga acgtcatcgc
     1801 tctgaagtcg ctgatggccg tcatcaaccc gcgatgccaa cggatcctct gtctgcgcag
     1861 gacacgaagt gtgtccggga cctcggtcca cacgacagcc gacggctcgt agacgatccg
     1921 gtagtcgtcg ccccgatcca tgtggagccg atgcaccctc atggtgagat ccaggtcttc
     1981 gcccatgtgc ccgcgggcga aacctccgac gtcgaccagg gtcgaacgct tccagatgcc
     2041 gaatgcgcct gagaccagtg ggagcgcgtt gatgcgcgac caggccgggc gactggcgag
     2101 gaacgtccga acgtactcga gcacttgcat cctggggatg agccgggtgg ggacattggc
     2161 cgagatgaga tggcccaact cgattcgact tccattcaac ggccgcacgt tgcctccgac
     2221 gccaactgtc cgctcgcgat cgtcaaccac ccggcgcatg gcatgaacca ggcagtcggg
     2281 ctcgagtatc acgtcggcgt ctgtgcagaa gacgtaggga aaacgggccg cgttgattcc
     2341 ggcgttgaga gcgtcggccc ggccaccgtt ctccttgtcg atcaccgtga tgtcgacccc
     2401 cgaccggccc ctgtatatgg cacgtatcgg ctccgactcg aggtcggggc ggtaggggat
     2461 acgaactttc tccagccgga acgccccgat cagcgtctcc aacgtcgcat cctgcgaccc
     2521 gtcgttggcg acgatgatct cgaatctcgg gtatttggcg atcgatatcg agcgcacggc
     2581 atcgacaatg cccgcttctt cgttgtaggc ggcgatgact atcgacaccg gtggtgcgtt
     2641 cgtgctggac agcatggccg tgccccggtt gaaccgacgt aggtggtggt ttcgcctcag
     2701 cacgaagccg gacagcacag tgaggccgag caggtacacc tgcatggcga tgaagtacag
     2761 gatcgttgcg aatcccagcc aggcgaagat gtccgcgacc atctcaggcc ccgctctcca
     2821 tgtgggcgat caagggctcc tcgacactga tgccggcagc gtggcggcgt cgggcggcca
     2881 ccagctcgcc gatgtctgca agcgcctccg cggcggcgtc ggcggcatag gcgtcgtcac
     2941 cgtcgaggg
//
LOCUS       DASUTA010000277         3125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_79882, whole genome shotgun sequence.
ACCESSION   DASUTA010000277 DASUTA010000000
VERSION     DASUTA010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79882"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(457..1443)
                     /locus_tag="VFZ80_05510"
     CDS             complement(457..1443)
                     /locus_tag="VFZ80_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6286922.1"
                     /translation="MESRTLGDSGLEVSALGYGCYGLSSGYGLPSQRDHAIAMIRAAV
                     ERGVNFFDTAQAYGPFTNEDVVGEALAPFRDEMVIATKFGHNFVDGEWQGLNSRPDHI
                     RRMTEDSLRRLRTDRIDLYYQHRVDPRVAIEDVAGTVKALVDEGKVKHFGLSEAGVAT
                     IRRAHAVHPVAALQSEYSLWWREPEDDILPVLEELGIGFVPFSPLGRGFLTGAIDADT
                     RFESSDIRNEIPRFDPENRRSNEGLIQLLQRMADRKKATPAQIALSWLLNRKPWIVPI
                     PGTTKLQRLEENLGAVDVDLVTEDEREMEQALAEIDIQGHRYSDAAQRMIDR"
     gene            1806..2846
                     /locus_tag="VFZ80_05515"
     CDS             1806..2846
                     /locus_tag="VFZ80_05515"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6286923.1"
                     /translation="MTKAEKWRTLAQVLAITVPMGMLIGFGLGWLIGGDSSSSLAAGT
                     LIGFLVAVGMVSFDVSWAVGLIPRGWREAPFLVVLVTRSFVWLAVIVIGISLPLLTVA
                     GVSFADLVDQQFIVVVAASFFAALLFNFVAQVNRLLGRRMLVRLILGRYHRPREEIRV
                     FLLIDVRGSTQIAERLGNLQYHAFLRRFISDVTGSVVRFRGEVHRYVGDEVILTWTAE
                     EGLSDARCVKVVFAISDTLETVRTEYEADFGIVPTFWAGLHLGPVVAGEIGTIKQEIA
                     FLGDTLNAAARIEQAGKELEHQFLASAAVISALDLPSDFATESLGRIELRGIEDSLEL
                     FAITRVADRMTV"
BASE COUNT          568 a          973 c          957 g          627 t
ORIGIN      
        1 gacaccacct ccgattgcag tcgagttgtt gacgactcgc attcactgac cacgtcgacc
       61 tccaggtcaa gacgaagctg gacggggccg ccaccaaggt catcaactca tgagacccat
      121 cgcggaccgt cgtggcgaga ctcacggttc aaccaggtgc tcagtttctg tggcacaccc
      181 atcccggtcc agtgctggtc aacgtagccc agggagtgtt ggtctacgtc caggcagatg
      241 attgtgtgga gcgctcctac ccggcaggca gagctacatc ggctcgccaa gatcgctcgg
      301 gccgaccggc ggagaatcca catcgatctc accaagtggt gcagcacggc gctaggacgg
      361 ctggggagaa tgctcgaatc gaggtcggct tctccgacgg ggccaaaccc gtaatgcaaa
      421 cggagcactg aagatctttg ctccggtcag cggcactcac cgatcgatca tgcgctgggc
      481 ggcgtcggag taacggtgtc cctggatgtc gatctctgcg agcgcctgtt ccatctcccg
      541 ctcatcctcg gtcacgaggt ccacatccac cgcgccgagg ttctcttcca gtcgttggag
      601 cttggtcgtg ccagggatcg gaacgatcca gggtttgcga ttcagcagcc atgagagagc
      661 gatttgcgct ggagtcgcct tcttacgatc ggccatgcgc tggagcagtt gtatcagtcc
      721 ctcgttggag cgcctattct cggggtcgaa tcgggggatt tcgttccgga tgtcagagct
      781 ctcgaaacgg gtgtcggcgt cgatggcacc ggtgagaaag cccctcccca gaggactgaa
      841 tgggacgaaa ccgatcccca actcctcgag cacggggagg atgtcgtcct cgggttcacg
      901 ccaccaaagc gaatactcac tttgaagcgc cgcaaccggg tggacagcgt gcgctctacg
      961 tatcgtcgcc acccctgctt cggagagccc gaagtgcttg accttcccct cgtcgactag
     1021 tgccttcacc gtgcctgcta catcctcgat tgcgacgcgt gggtcgaccc ggtgttggta
     1081 gtagaggtcg atgcggtcgg tgcggaggcg acgtagcgag tcctcggtca tccttcggat
     1141 gtggtccggc cggctgttca ggccctgcca ttcgccatcg acgaagttgt ggccgaactt
     1201 ggtggcaatc accatttcgt cgcgaaaagg agcgagtgcc tcgccgacga catcctcatt
     1261 cgtgaatggc ccgtaggcct gggcggtgtc gaagaagttg acgccgcgct cgaccgcagc
     1321 gcgaatcatg gcaatggcgt ggtctcgctg cgacggtaag ccgtagccgg agctgagccc
     1381 ataacagccg tagccgagag cggagacctc gaggccgcta tctccgaggg ttcgtgattc
     1441 cattctcctc tagccccgtg gtgggtcgcg tcgaagccca atgcgaccac cgggcatccc
     1501 tctgcggttt ttgccgggca tcgagccaat agtggcggag cccggcatcg aatgcgatct
     1561 cgatcgcatt gcgtccgccg cggcccggtc cggcgaatac cccggtacca gggttgcagg
     1621 ctacgctcag cgaagtctcc ggccccccca gaaagacgcg agcggctcgt gttctatcga
     1681 gatggcagag ccaatacttg gtcctcggcc tccggtagac ccatggaaag gcgtcgacgg
     1741 ggaagtccga tcccggctgt cagcactcct acgcttggcc acccgaagcg aggtatcgca
     1801 agccaatgac gaaagcggag aagtggcgca ccctggcgca ggtcctggct atcacggtcc
     1861 ccatgggaat gctgatcggg ttcgggctcg gctggctcat cggcggagac tcgagctcgt
     1921 cgttggcagc aggaactttg atcggtttcc tcgtcgccgt ggggatggtg agcttcgatg
     1981 tgagctgggc ggtaggcctc atcccccggg gttggcgtga ggctcccttt ctcgtggtgc
     2041 tggtcacacg gagtttcgtg tggctggcgg tcatcgtcat tgggatcagc ctcccgctgc
     2101 tcaccgtcgc tggcgtgtcg tttgccgacc tggtcgacca gcaattcatc gttgtcgtcg
     2161 ccgccagctt cttcgccgct ttgctgttca acttcgtggc tcaggtgaat cgactcctcg
     2221 gccgcagaat gctggtccgt ctcattctcg gccgatacca ccggccccgc gaagagattc
     2281 gagtcttctt gctcatcgac gttcgcggat ccacccagat cgccgaacgg ctcggaaacc
     2341 tccaatatca cgcctttctg agacgattca tcagtgacgt cacaggaagc gtggttcgct
     2401 tccgcggaga ggtgcaccgc tacgtgggcg acgaggtgat cctgacgtgg accgcagaag
     2461 agggtctgag cgacgcccgc tgtgtcaagg tcgtctttgc catctccgac accctggaga
     2521 cggtgcgcac cgagtacgaa gccgacttcg gcatcgtgcc caccttctgg gccggtctcc
     2581 atctcggtcc cgtggtcgcc ggcgagatcg ggacgatcaa acaggagatc gcatttctcg
     2641 gagacaccct gaatgccgcg gcgcgaatcg aacaagcggg caaagagctc gaacaccagt
     2701 ttctcgcttc ggcagccgtc atctcggcgc tcgacttgcc gagcgacttc gcaacagaga
     2761 gcctcggccg gatcgagctg cgcggaatcg aggactccct cgagctcttc gccatcacac
     2821 gcgtcgccga tcggatgacc gtctgaaccg cggccgagtt ccgagctgct cgctaccaat
     2881 ctgacagccg tcagacgtaa ggaccggttc agaagcgtcc tgacggcgac taccggctag
     2941 aggtatccca tctcctgggc tttggcgaac gcctctgcgc ggttgcgtgc agcggtccgt
     3001 aaccattgct cagtgtcctc cgtcatcgga gtttcgctca gaacaaccga gccgaaccaa
     3061 ggcgaccgcg ctgcaagttc attgagtcca gaggttctac ctgactccag agatccatgc
     3121 atcag
//
LOCUS       DASUTA010000278         4907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1376183, whole genome shotgun sequence.
ACCESSION   DASUTA010000278 DASUTA010000000
VERSION     DASUTA010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376183"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFZ80_05520"
     CDS             complement(<1..229)
                     /locus_tag="VFZ80_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEX6286924.1"
                     /translation="MRDLYPEIEPFDSDTLQVDDRHSLYYEQCGNPDGKPVVLLHGGP
                     GAGCNAKMRRFHDPAKYRIVLFDQRGAGRSTP"
     gene            complement(475..2106)
                     /locus_tag="VFZ80_05525"
     CDS             complement(475..2106)
                     /locus_tag="VFZ80_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6286925.1"
                     /translation="MAEKTRSAAGTVLLTLAAGQFLMTLDTSVMNVAIAQVAEDVGTT
                     VTGIQTAITLYALVMAALMITGGKIGSLIGRRRAFAIGCIIYGAGSLTTALAPNLTVL
                     IIGWSFLEGTGAALIMPAIVALVASNFPPEGRPRAYGLVASAGAIAVAAGPLIGGAFT
                     TYASWRYVFAGEVLIVIAILALSRRMIDVPPETRFRLDLVGSALSATGLALAVFGVLR
                     SGEWGWLRPTPSGPDVLGIAPTFWLILAGLLVLWLFVAWEERVIAAGREPLIRPAMLQ
                     NAQLEGGLIMFFFLFMIQMGMFFVVPLFLSVSLGLTAMATGVRLLPLSITLLLAAVAI
                     PKYMPQASPRRVVRWGLLALFAGIVSLIAGLEAGAGPEIVTVPLLLAGLGIGAMVSQL
                     GAVTVSAVSDEESGEVGGLQNTMTNLGSSIGTALAGSLLIAGLTAAFIQGIQENPDIP
                     PELTATAEVELASGIPFVSDAQLEEALAATDLPANDAAAIMDENELARIAGLRMSLSV
                     LALAALVALFLSRMIPSEPVGSSSGAAEESSVPSR"
     gene            complement(2210..2518)
                     /locus_tag="VFZ80_05530"
     CDS             complement(2210..2518)
                     /locus_tag="VFZ80_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6286926.1"
                     /translation="MVRSARNSTPSLRPWSRWRLWSLESNIIAPKVMGHSVQLPPIVV
                     LISITAAYQVAGILGAIVAVPVMANAKISLCYVWAKIQSRDPFPSPRLSPAQEGTALE
                     "
     gene            2826..3962
                     /locus_tag="VFZ80_05535"
     CDS             2826..3962
                     /locus_tag="VFZ80_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6286927.1"
                     /translation="MTTRSPDKSSTGIADLIDPDRLLATFLELVAIDSPTGHEEQIGR
                     ELSERFTALGCQVRTDDIGNLVATFPGPGTDTILISTHMDTAGTDVGIEPIIEDGIIR
                     TDGSTILGADDKSGLAGCLELLTLLNANPDVEHPTIEFVVSVGEESGLVGSRQMDVGM
                     LKSSHGYVFDTAGAISAITYWAPTSVYITVTVHGKKVHAGVEPEKGINAIKVAAEAIA
                     AMPLGRIDEETVANIGTINGGEARNIVPGEVVLQGMARSHDQAKLDAQLDAMRTAFEE
                     AATRHGAAVDFEAEEIYRTYRIDESERPYRDAARAIMSLGLEVVPRKSGGGTDGNFFN
                     AKGIPCVAMATGMVDEHATTEHIAIDDMVTACRILVTIVTQEPE"
     gene            3973..4389
                     /locus_tag="VFZ80_05540"
     CDS             3973..4389
                     /locus_tag="VFZ80_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286928.1"
                     /translation="MTKLGRKMTIATTAVLILGLTGCGNVEERSLCRQYEDLREAAAQ
                     IDNLDPETATVADAAAIVEDVMVQLDQFQAASEGIYDQAVSNLNLALTELRQATFDIG
                     DESLEVARPLLEESWQNTLTAYSILEQRLDVVCGTD"
     gene            4406..>4907
                     /locus_tag="VFZ80_05545"
     CDS             4406..>4907
                     /locus_tag="VFZ80_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003940851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter family
                     protein"
                     /protein_id="HEX6286929.1"
                     /translation="MDIVFILEALVVLGAIVMGTRAGGVGIGLWGGLGVAMLVFVFGE
                     APGEAPIAAMLIILSVVTAASLMQVAGGVDWMVKVAAGVIQRHPKQITTIAPLTAFAF
                     TVGAGTSNILYPLLPVIYDVSYQNNIRPSRPLTVTVVQSGMSLAASPVSAAMAAMLTL
                     TAVEPFN"
BASE COUNT         1002 a         1517 c         1540 g          848 t
ORIGIN      
        1 ggggcgtcga gcggccggcg ccgcgctgat cgaacagcac gatccgatac ttggcggggt
       61 cgtgaaagcg ccgcatcttt gcgttgcagc cggcacccgg gccaccgtga agcaacacca
      121 cgggcttccc atcggggttg ccgcactgct cgtaatagag actgtggcga tcgtcgacct
      181 gaagtgtgtc actgtcgaat ggttcgatct cggggtatag gtcgcgcagc gaagccgagc
      241 tgggctgagt ctcagtcatg attatgggtc ctttcgatcc agcatccgat tctctccctg
      301 tctctaccgg ggtgccatcc tcctgtttgg gtgactcagc agagactcat cgttgcgggc
      361 attgacggac gcgaagtcat cgctcagtgg ggagaacgcg gcagttcggc cggttgtatc
      421 gccagagggc tcactgaagc ggagtacgcg tgcgtttcag gcttggcagt cttttcagcg
      481 gctggggaca gacgattcct cggcggcgcc tgacgagcta cccaccggct cgctcgggat
      541 catccgggat aggaagaggg caaccaatgc ggccagcgcc aacaccgaga ggctcatccg
      601 cagcccggcg atcctggcca actcgttctc atccatgatc gcggcggcgt cattggctgg
      661 caggtcggtt gcggccaatg cttcttcgag ctgcgcgtcg gagacgaacg gaatgccgct
      721 ggcgagctcc acttctgctg tggcggtcaa ttcgggaggg atgtccgggt tctcctgtat
      781 cccctggatg aaggcggcgg tcaggccagc gatgagcaac gaaccggcga gcgcggtgcc
      841 aattgacgat ccgaggttgg tcatggtgtt ctgaaggccg ccgacctcgc cgctctcctc
      901 gtcagaaacc gcggagactg tgaccgcacc cagctgcgag accattgcgc cgatacccag
      961 acctgcgaga agcagtggca ccgtgacaat ttccgggccg gcaccggcct cgagcccagc
     1021 gatgagcgag acgatcccgg caaagagggc gagcaaaccc caacgcacca ccctgcgtgg
     1081 cgaagcctgg ggcatgtact tgggaatcgc cacggcagcg aggaggagcg tgatcgacaa
     1141 tgggagcaac cggacgcctg ttgccatggc agtgagaccg agcgacaccg acaggaacaa
     1201 cggcaccacg aagaacatcc ccatctggat catgaacaag aagaagaaca tgatcagccc
     1261 gccctcgagt tgggcgttct gaagcatggc gggccgaatc agaggctccc gcccggcggc
     1321 gatgacgcgc tcctcccatg cgacgaacag ccaaagcacg agcaaccccg ccagaatcaa
     1381 ccaaaaagtc ggtgcgatcc ccaagacatc cggtccactt ggcgttggcc tcaaccaccc
     1441 ccattccccg gagcgaagga cgccgaacac cgccagggcg agcccggtcg cggagagggc
     1501 cgaccccacc aggtccaacc ggaatctggt ctccgggggc acgtcgatca tccgtctcga
     1561 cagcgccagg atcgctatga cgatcaatac ctcacccgcg aagacatagc gccaggaggc
     1621 gtaggtggtg aacgctcccc cgatcagcgg tcccgcggcc accgcgatcg cacctgcgga
     1681 tgccaccaga ccgtaggccc gtggcctccc ctcgggtgga aagttcgagg ccaccagagc
     1741 aacgatcgcc ggcatgatca gggccgctcc cgtaccctcg aggaaagacc agccgatgat
     1801 gagaacggtc aggttgggag ccagcgcagt ggtcagagag cctgccccgt agatgatgca
     1861 tccgatggca aaggcgcgcc ggcggccaat gagactcccg atcttccctc ccgtgatcat
     1921 cagagccgcc atgaccagcg catagagggt gatcgccgtc tggatcccgg tgaccgtggt
     1981 gcccacatct tcggcgacct gggcgatcgc cacgttcata acggatgtgt ctagtgtcat
     2041 caggaattgg cccgccgcca gggtcaatag gacggtgccg gccgcgcttc tggtcttctc
     2101 tgccaccgag ccaccttacc gatcgaatgt tggctgatcg ggaagctccg ttgctcggcc
     2161 atcctcgagg aaggttgagg ccgaggccgc agtcgaccct ggtgcggtac tactcgagag
     2221 ctgtaccttc ctgggctgga ctcagccttg gtgacggaaa aggatccctt gattggatct
     2281 tggcccagac atagcagagg gagatcttgg cgttggccat caccggaacg gcgacgatcg
     2341 ccccgagaat cccggcgacc tgatacgcgg cggtgatcga gatcagaacc acgatgggag
     2401 ggagctggac ggagtgcccc atcaccttgg gagcgatgat gttgctctcg agagaccaaa
     2461 gccgccatct cgaccagggc cgcaggcttg gggtcgagtt ccgtgccgac ctgaccaagc
     2521 atcaacctca tcgattgtgg cctcatcgcc gaaagtcaga cctcgcaaca gccgttggag
     2581 gtgctcagga atcgacatac gctccacctc tcgtggcatc cgcctcttca ggcagtcgca
     2641 tcattcgagc atgcaaccca catgtcatcg cccggcgtgg atgatgtctc gacctgatca
     2701 aagggggacg acacacgtat tcgtcgccga ccactccgtc ttttcatcca tcgtggacga
     2761 tgcccggaac tctgcagcga gttagttacg ggttgtgacc aacgaaagcc gaggaggtga
     2821 tagatatgac gacgcgctca cctgacaaga gctccacagg aatagctgat ctgatcgacc
     2881 cggaccgcct actggcgaca ttccttgagt tggtggccat agacagcccg acagggcatg
     2941 aggaacagat cggtcgcgag ctttcggagc gtttcaccgc actcggctgt caggtgagga
     3001 cggacgacat cggcaacctg gtcgccacat tccccggtcc cgggacggac accatcctga
     3061 tctccaccca catggatacc gcaggaaccg acgtcggaat cgagccgatc atcgaagacg
     3121 gcatcatccg cactgacggg tcgacgatcc tcggtgccga cgacaagtcc gggctcgccg
     3181 gatgcctgga gctacttacg ttgctcaacg cgaaccccga tgtcgaacat cccaccatcg
     3241 agttcgtggt gtcggttggg gaagagagcg ggctcgtggg ttcccggcag atggacgtgg
     3301 gcatgctgaa gtcgagtcac ggctatgtct tcgacactgc cggagcgatc agcgccatca
     3361 cctactgggc acccacttcg gtgtacatca ccgtgaccgt gcatggcaag aaggtccacg
     3421 ccggtgtcga gcccgagaag ggaatcaacg ccatcaaggt ggcggcagaa gccattgctg
     3481 cgatgcccct gggtcggatc gacgaggaga ccgtggccaa catcggcacc atcaacggcg
     3541 gcgaggcgcg caacatcgtg ccgggcgagg tggtcctcca gggaatggcc cgcagccacg
     3601 accaagccaa gctcgacgca cagctcgatg cgatgcgcac cgcattcgag gaggccgcga
     3661 cccgacacgg tgccgcggtg gacttcgagg cagaggagat ctaccggacc taccgcatcg
     3721 acgagtccga gcgaccatat cgagatgccg cccgggccat aatgtcgctc gggttggagg
     3781 tcgtcccccg gaagagcggg ggcgggaccg acggcaactt cttcaatgcc aagggaatcc
     3841 cctgtgtcgc tatggccacg gggatggtcg acgagcacgc caccacggag cacatcgcga
     3901 tcgacgacat ggtcaccgca tgcaggatct tggtgacgat tgtcactcag gaaccggaat
     3961 gaaagagggg ccatgaccaa gctcggccgg aagatgacga ttgccaccac tgcagtgctg
     4021 atcctggggc tcaccggatg cggcaacgta gaggagcgca gtctctgtcg gcagtacgaa
     4081 gaccttcgag aagcagcagc ccagatcgac aacctcgacc cggaaaccgc aaccgtcgct
     4141 gatgcggcgg cgatcgtcga ggatgtgatg gtccaactcg accagttcca ggcagcttca
     4201 gagggcatct acgaccaggc agtgtcgaac ctgaacctcg ccttgaccga gttgaggcaa
     4261 gcgacgttcg atattggcga tgagagcctc gaggtggcac gaccgctcct cgaggagtca
     4321 tggcagaaca ccttgacggc ctactcaatc ctcgaacagc gactggacgt cgtctgcggc
     4381 accgactgac ccgaaaggaa tcaaattgga catcgtcttc atcctcgagg cactggtggt
     4441 actcggggcc atcgtcatgg gtacccgcgc aggcggagtc gggatcggtt tgtggggtgg
     4501 tctcggtgtc gccatgctgg tcttcgtgtt cggcgaggcg cccggggagg cgccgatcgc
     4561 agcgatgctc atcattctct cggtggtgac tgctgcgtcg ctgatgcagg ttgcaggcgg
     4621 ggtcgactgg atggtgaagg tggctgcggg ggttatccaa cgccacccca agcagatcac
     4681 gaccattgct cccctcaccg ccttcgcttt cacggtgggc gccggcacga gcaacattct
     4741 ctatccgctg cttccggtga tctacgacgt ttcgtatcag aacaacatca ggccgtcgcg
     4801 tcctttgacg gtcactgtgg tccagtcggg gatgtcactc gccgcgtcgc cggtctccgc
     4861 ggcgatggcc gcgatgctga ccctgaccgc agtcgagccg ttcaact
//
LOCUS       DASUTA010000279         3444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1764808, whole genome shotgun sequence.
ACCESSION   DASUTA010000279 DASUTA010000000
VERSION     DASUTA010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1764808"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2114)
                     /locus_tag="VFZ80_05550"
     CDS             complement(<1..2114)
                     /locus_tag="VFZ80_05550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX6286930.1"
                     /translation="MSDNPFRLPRHATPTHYDIRLELDLETFTFVGTVAVDVDVHEPT
                     DALVLNAAEVEIKSASLSNGVEITEIAYDDEKERATLHLGSSLEPGSVRLEMEHSGII
                     NDQLRGLYRSVYRDAEGVEHPLATSQCQSTDARRVFPCWDEPDFKATYQTTMVVADGL
                     EAYSNTSELARTRLDDGRVEFRFDRTMKMSTYLLAFIAGPFEATEPRMVRGTPIRVIV
                     PRGNLHLTDIALENAVFCFEYLSDYYGIPYPADKLDHIAIPDFAAGAMENVGLITYRD
                     AYLVIDPVKASQGELQNSLDVIGHEIAHQWFGNLVTMAWWEGAWLNEAFASFMELKAT
                     DAMRPEWKRWLAFANLEVPWAMGTDQLQSTRPIEFEVTAPEEVDQMFDAITYGKGSAV
                     LHMIDEFIGVENFRKGVGNYLRKHEYANTVTSDLWEGLDGASEYPVSEIMDTWVYQRG
                     FPQIDVRTVDGGVQLSQRRYLVIPDETDTTIWKVPIQLRGVADGRPFEIKYLLEDDEG
                     VVAIDGSIEWVVANAGGFGFYRTSYSDDLFGALLEHVDSLGDNERHALVSDTLGFVRN
                     GQLSTTNFLDLVAQFTDEDEQAIWSVITGGLGLIEHHALEEGARKGFEKFVSDLVGPA
                     LGRLGWEVRESDSDLDRKLRGDLIATMGNLATDGATIDRCRTLVTDLLRGEPMDPEVA
                     TAALAVYARNGSAEEYETLWRV"
     gene            2214..3440
                     /gene="serB"
                     /locus_tag="VFZ80_05555"
     CDS             2214..3440
                     /gene="serB"
                     /locus_tag="VFZ80_05555"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEX6286931.1"
                     /translation="MADRSTILVNVHGPDHPGISAGLMDVLTATHAEVYDIEQIVVRG
                     RLTLNVLIGVDGEKATIRDLLLFGWEQRLQIEFEVMDPLPTPLSVMTVVTVLGERIGP
                     DDFGAVARAIAEGGGNIERIFRLSRYPVVSYELTVSNGDIHKIREQLIAAAAERPIDI
                     AIQPEGLERRAKRMVVMDVDSTLIENEVINLLAEEAGVGEKVSLITAAALRGEIDFEG
                     SLRERVALLEGLDADALQRVSKRITVTPGARTFIRTLKRMGMKTAIVSAGFTRFADAL
                     AADLGIDYSLSNTLDVDDGVLTGKLAGELVDGPRKAAFLLEIAKKEKINPSQVVAVGD
                     GANDLEMLAAAGLGIAFNAKPVVRERADTSVSVPYLDAILFLMGVRRDYVEAADAADP
                     EFKQGPLLEVPGTPPA"
BASE COUNT          595 a         1073 c         1142 g          634 t
ORIGIN      
        1 tagacccgcc acagggtctc gtactcctcg gcgctgccgt tgcgggcgta gaccgccagg
       61 gccgccgtgg ccacctccgg gtccatgggc tccccgcgga gcaggtcggt cacgagggtg
      121 cggcatcggt cgatggtggc cccgtcggtg gcgagattgc ccatcgtggc gatgaggtcg
      181 ccccgaagct tgcggtcgag gtcgctgtcc gactccctga cctcccagcc caatcggccc
      241 agcgccgggc cgaccaggtc ggagacgaac ttctcgaagc ccttacgggc cccctcctcg
      301 agggcgtggt gctcgatgag cccgagccct ccggtgatca cggaccatat ggcctgttcg
      361 tcctcgtcgg tgaactgtgc cacgaggtcg aggaagttgg tggtggagag ctggccgttg
      421 cggacgaatc cgagagtgtc ggacacgagg gcgtggcgct cgttgtctcc gagcgagtcg
      481 acgtgctcga gaagggcccc gaacaggtcg tcggagtagc tggtgcgata gaacccgaag
      541 cctccggcgt tggcgaccac ccactcgatg ctcccgtcga tggcgacgac gccctcgtcg
      601 tcctcgagga gatacttgat ctcgaagggc cgcccgtcgg ccacaccccg cagttggatc
      661 gggaccttcc agatggtcgt gtcagtctcg tcagggatca ccaggtagcg tcgttgtgac
      721 agttgcaccc cgccatcgac ggtcctgacg tcgatctgag ggaaccctcg ttggtagacc
      781 caggtgtcca tgatctcgga caccgggtac tccgatgccc cgtcgagacc ttcccagagg
      841 tcgctggtga ccgtgttggc gtattcgtgc ttgcgtaggt agttgccgac acccttgcgg
      901 aagttctcga ccccgatgaa ctcgtcgatc atgtggagga ccgccgaccc ctttccgtag
      961 gtgatggcgt cgaacatctg gtcgacttcc tccggggcag taacctcgaa ctcgataggc
     1021 cgggtcgatt gcagctggtc ggtgcccatg gcccatggga cttcgaggtt ggcgaaggcc
     1081 agccaccgtt tccactcggg acgcatcgcg tcggtggcct tcagctccat gaagctggcg
     1141 aaggcctcgt tgagccaggc accctcccac caggccatcg tcaccaggtt gccgaaccat
     1201 tggtgggcga tctcgtggcc gatgacgtcg agactgttct gcaactctcc ctggctcgcc
     1261 ttgaccgggt cgatcacgag ataggcgtcc cgataggtga tcaggccgac gttctccatc
     1321 gccccggcgg cgaagtcggg gatggcgatg tggtcgagct tgtcggcggg ataggggatt
     1381 ccgtagtagt ccgagaggta ctcgaagcag aacacagcgt tctccagggc gatgtctgtg
     1441 agatggagat tgccccgcgg gacgatgacc cggatcggtg tgcctcgcac catccgcggc
     1501 tcggtggcct cgaacggccc ggcgatgaat gccaggaggt aggtcgacat cttcatggtc
     1561 cgatcgaatc ggaactcgac acgaccatcg tcgagacggg tccgcgcaag ctcggaggta
     1621 ttcgagtagg cctccaggcc atcggccacc accatcgtgg tctgatacgt cgctttgaag
     1681 tccggctcgt cccagcaggg gaagacccgc cgggcatcgg ttgactggca ctgggaggtg
     1741 gcgagcgggt gctcgacccc ctccgcgtcg cgatacacgg accggtagag accgcgcagc
     1801 tggtcgttga tgatcccgga gtgctccatc tccagccgga ccgagcccgg ctccagggac
     1861 gagccgagat ggagagtggc tcgctccttc tcgtcgtcgt aggcgatctc ggttatctcg
     1921 accccgttcg acaaggaagc cgacttgatc tcgacctcgg cggcgttgag gacgagggca
     1981 tcggttggct cgtggacgtc tacgtctaca gcgacggtgc cgacgaaggt gaacgtctcc
     2041 agatcgagct cgagacggat gtcgtaatgc gttggcgtcg catgtcgcgg cagccgaaac
     2101 gggttgtcag acaaagggat tcctcatgtt gggtggacag ggggaatggt accggtgctc
     2161 ggcggttggt cgaagtggac ttggcccttt ccggccctaa cgtgtacggc cggatggcgg
     2221 atagatcaac gattctcgtc aacgtccacg gccccgatca ccccgggatc tcggccgggc
     2281 tcatggacgt gctgactgcg acacacgccg aggtctacga catcgagcag atcgtcgtga
     2341 ggggtcgtct gaccctgaac gtgctcatcg gcgtggatgg cgagaaggcc acgattcgcg
     2401 atctcctcct cttcggttgg gagcagcgcc tccagatcga gttcgaggtg atggatcccc
     2461 tgcccacccc cctgagcgtg atgaccgtcg tcaccgtcct cggagagcgg atcggccccg
     2521 acgacttcgg tgcggtcgcc cgtgccatcg ccgagggtgg gggcaacatc gagcggatct
     2581 tcagactgtc ccgatacccg gtggtgtcct acgagctgac cgtgtcgaac ggggacatcc
     2641 acaagatccg cgagcaactc atcgcagcgg ccgcggagcg acccatcgac atcgccatcc
     2701 agccagaagg gctggaacga cgggcgaagc gaatggtcgt gatggacgtc gactccaccc
     2761 tgatcgagaa cgaggtgatc aacctgctcg ccgaggaggc cggtgtcggg gagaaggtgt
     2821 ctctcatcac tgccgccgcc cttcggggcg agatcgactt cgaggggtcg ctgcgcgagc
     2881 gcgtcgccct actcgaaggt ctcgatgcgg acgccctgca gcgggtgtcc aagaggatca
     2941 cggtgactcc gggagctcga accttcatcc ggaccctgaa aagaatgggc atgaagacgg
     3001 cgatcgtctc cgctgggttc acccgtttcg ccgatgcact ggccgcggac ctgggcatcg
     3061 actactcgct ctcgaacacc ctcgatgtag atgacggtgt gctcaccggg aaactcgccg
     3121 gcgagctggt cgatggcccc cgcaaagccg ccttcttatt ggagatcgcc aagaaggaga
     3181 agatcaaccc gtcccaggtg gtggcggtcg gcgacggggc caacgatctc gagatgctgg
     3241 ccgcggcggg tctgggcatc gccttcaacg ccaagccggt ggtcagggag cgggccgaca
     3301 catccgtctc ggtcccctat ctcgacgcca tcctcttcct gatgggggtg cggcgggact
     3361 atgtggaggc tgctgacgcc gccgatcccg agttcaaaca gggaccgtta ctcgaggtcc
     3421 ctggcacccc acccgcctga ggcc
//
LOCUS       DASUTA010000280         2641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_987182, whole genome shotgun sequence.
ACCESSION   DASUTA010000280 DASUTA010000000
VERSION     DASUTA010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987182"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1745)
                     /locus_tag="VFZ80_05560"
     CDS             complement(<1..1745)
                     /locus_tag="VFZ80_05560"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX6286932.1"
                     /translation="MTPDQIAELRIPSDPRLSPDGGRVVYVVSRPDLEEDRYDRSLWV
                     DDRQFTEGPGDSSPRWSPDGKLLAFLRSVDGEPAQIAIMPIEGGEPRLITDYELGVES
                     LEWSPDGERLVAVVVEWVDEWADLDKEERSRRPRRIETVPHRFDNKGWIHDRKRHLWI
                     IEVNGRGDPERLTEGSFDEESPSWSPHGDKIAFISDRDPGQGLVSGNDVWEVDVASRE
                     TSQVTERGFWTVVSYRHDGALHLLGNTNPRYPIDSYLYRREEDGRLTDLTGHLDRGSV
                     SLAAGPAAVKWDGDNAVLGLEDSGTFGLIEVSPDGSVETLVDDRAVVTGFDRAGDRLV
                     YTASTWESPGELFSNGDASTQLNTGSGLDLIRPDHFRVESDGHDIDVWGYLPEGTDPV
                     PFLLNIHGGPASQYGFGFFDEFQVYAAAGYGVVACNPRGSAGRGQEFVCAVKGEGWGV
                     VDYADLKVAVEAALERHDRLDADRMGVMGGSYGGFMTAWIIGQEDRWKSAVVERALIS
                     WTSFAGTSDIGGVFPENYLEVPYPDAWGTWWEKGPLALAHNVTTPTLVLHAENDFRCP
                     IEQAEQYFMALLRNGT"
     gene            complement(2127..2402)
                     /locus_tag="VFZ80_05565"
     CDS             complement(2127..2402)
                     /locus_tag="VFZ80_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286933.1"
                     /translation="MPKRQTGLVVGKDFRPPDGAQPMDWCYLTVDISDAERISVRIRR
                     DQVSRADVGDVVQFKPPRDDQHTVSRLTRLGSDPGLLPPVDRARPRA"
BASE COUNT          475 a          843 c          820 g          503 t
ORIGIN      
        1 gtgccgttgc gaagcagcgc catgaagtac tgctcggctt gctcgatcgg gcatctgaag
       61 tcgttctcgg cgtgaagcac gagtgtgggc gtcgtgacgt tgtgagccag ggcgagcggg
      121 cccttttccc accaggtgcc ccaggcgtcc gggtagggca cctcgaggta gttctccgga
      181 aacactcctc cgatgtcgga ggttcccgca aagctggtcc acgagatcaa tgctctctct
      241 accacggccg acttccaccg gtcttcctga ccgatgatcc acgcggtcat gaatcctcca
      301 tacgacccac ccatgacacc catgcgatcg gcgtcgaggc ggtcgtggcg ctccagagcc
      361 gcctcgacag ccactttcag atcggcgtaa tcgaccacgc cccagccttc tcctttgact
      421 gcgcagacga actcttggcc gcgccccgcc gacccacgcg ggttgcaggc aacgactccg
      481 tatccggcag cggcgtagac ctggaactcg tcgaagaagc cgaacccgta ttgcgaagcg
      541 ggccctccgt ggatgttgag aagaaagggg accgggtcgg tgccttccgg caagtagccc
      601 cagacgtcga tgtcgtggcc gtcggattcg acccggaagt gatcgggtct gatcaaatcg
      661 agacccgaac cggtgttgag ctgcgtcgag gcgtcaccat tcgaaaagag ctcgccgggt
      721 gattcccagg tcgacgcggt gtagacgagt cggtcgccgg ccctgtcgaa gccggtcaca
      781 acggccctgt cgtcgaccag agtctcgacc gaaccgtccg gtgacacttc gatcagcccg
      841 aacgtcccgg aatcctcgag accgagcacg gcgttgtcgc catcccattt gaccgccgcc
      901 ggccctgcag ccaaagacac agagccacga tcgaggtgtc cggtcaggtc ggtgagtcgg
      961 ccgtcttcct cccgccggta gagataggag tcgatcggat agcgggggtt ggtgttgcca
     1021 agcagatgca gtgcgccgtc gtggcggtac gagacaactg tccagaaacc cctctcggtg
     1081 acctgcgacg tctctctgct ggccacgtcg acctcccaga cgtcattccc cgagacgagc
     1141 ccctggccgg gatctcgatc ggaaatgaat gcgatcttgt ccccatgagg cgaccatgac
     1201 ggcgactcct cgtcgaacga gccttcggtg agacgctccg ggtcccctcg accgttgact
     1261 tcgatgatcc agagatgtcg cttgcgatcg tggatccaac ccttgttgtc gaagcgatgt
     1321 ggcaccgttt cgatccgtcg ggggcggcgg gatcgttcct ctttgtcgag gtcggcccac
     1381 tcgtcgaccc actcgaccac gacggcaacg aggcgttcgc catccggtga ccactccaga
     1441 gattccacgc ccaactcgta gtcggtgatc aaccgaggtt cgccgccttc gatcggcatg
     1501 atcgcaatct gcgccggctc accatccacg ctcctgagaa aagcgagcag cttcccgtct
     1561 ggcgaccacc gcggtgagct gtcaccggga ccctcggtga actggcggtc gtccacccag
     1621 agagaccggt catacctgtc ttcttccagg tctggccgag acacgacgta gacgaccctg
     1681 ccaccgtcgg gtgagagtcg tgggtcggag gggattctga gctcggctat ctggtcggga
     1741 gtcatggagg cgaagataac gtcgataccg tcgcaggctg ttcgacagac gacagcacga
     1801 tcagagtgga ttgcagtggc cgtgaagcgg acgtcgagat cgtccatctc gaccaggcga
     1861 tcggcctgct gactgccagc ccgggtcggt cgatcgagtc aagcgatcgg aagaggtctc
     1921 actcccggag aacaccgtgg ccgactgccc gatcctcgtg atcggaggag gagcggacgg
     1981 ggtcgtcaca cagagcaccc ggcctgctat ctcgtcgagc atcctgacgc cgagtacttg
     2041 atcgtggatg acgcgtcaca cggttgcatc atggaagacc ctggctcctt cgcttcgata
     2101 gtccaccagt tcgtctcgaa gatgagctag gcccttggcc gcgccctgtc taccggaggg
     2161 agaagccccg ggtcgctacc cagccgggtc agtcgggata cggtgtgctg gtcgtctcta
     2221 ggcggcttga actggacgac gtcgccgaca tcggctcgtg agacctgatc tcggcggata
     2281 cgcaccgaaa tccgctccgc gtcggagatg tcgacggtca agtagcacca gtccatcggt
     2341 tgggcgccgt ccggtggccg aaagtccttc ccgaccacca atccggtctg tcgcttgggc
     2401 atgtcagctc cttctctgga gcctctcagc ccatccctga cgggtgcctg ctcacattct
     2461 acgtcagggt gtcacgcagg ggaagggttg ggtcttgaag ccaacgcaac gggtgttact
     2521 ttgttgcgcg ggcggtatgg gggagaggag aacgccttgc cgcgcaagag cctcgtggtt
     2581 ctcgtggtca tcgccctggc cgcagtcgcc atccctgttt ccgccgcgcc cgagtctgtc
     2641 g
//
LOCUS       DASUTA010000281         3099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1117765, whole genome shotgun sequence.
ACCESSION   DASUTA010000281 DASUTA010000000
VERSION     DASUTA010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117765"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /gene="ribB"
                     /locus_tag="VFZ80_05570"
     CDS             complement(<1..507)
                     /gene="ribB"
                     /locus_tag="VFZ80_05570"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004671523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX6286934.1"
                     /translation="MRFAPIEDIVAAIGRGEMVIMVDDEDRENEGDLIVAAEDATPDN
                     IGFMLRYTSGIICLPVVGERLDELELPMMVAKNTDVRRTAFTISIDAAHGTTTGISAA
                     DRCRTVLAVIDPETKPEDLARPGHMYPLRYEPGGVLKRAGHTEAAVDLAELAGKYPAA
                     VLAEVMNDD"
     gene            complement(504..1094)
                     /locus_tag="VFZ80_05575"
     CDS             complement(504..1094)
                     /locus_tag="VFZ80_05575"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX6286935.1"
                     /translation="MFTGIVEQIGEVVSLEETASGRRVVLRGAGLGELRVGASLAVNG
                     VCLTAIAPRGDTVSLDVIPETLGRTNLGRLEKGSQVNLERPMPASGRFDGHIVQGHVD
                     GTGTIQAIRSSDDGSVIMEVAVSPDMLRYLVEKGSVTVDGVSLTVASVSENTFTVALI
                     PHTLEVTTLGLRGEGDTVNLEMDVLAKYVERLLDRR"
     gene            complement(1095..2138)
                     /gene="ribD"
                     /locus_tag="VFZ80_05580"
     CDS             complement(1095..2138)
                     /gene="ribD"
                     /locus_tag="VFZ80_05580"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX6286936.1"
                     /translation="MTESRFLQRTYDLARTQHPHPNPRVGAIVVDRHGEIVGEGAHEG
                     PGSPHAEALALERASNVLGSTVYVSLEPCVHHGLTPPCVDRLIDEGVGRVVIGALDPD
                     PRVSGAGVESLRQAGIDVLVVDDPGAREVDPAYFHHRERGMPLVTLKYAMTLDGSVAA
                     LDRTSQWITSEEARNDAHSLRAANDAVIVGTGTLVEDDPRLDVRLPDYQGPQPRPVIV
                     AGESELPHDRAIWERNPVVVAPGDMSIPSGDLLVVDGPGRPGPETVCRALADAGFLSV
                     LLEGGPTLAGAWMRAGVVQRGVVYIGGRIGGGVGRSPLGGRFGSIGEAESAKIIDVRK
                     LGPDLRVDFALDG"
     gene            complement(2135..>3099)
                     /locus_tag="VFZ80_05585"
     CDS             complement(2135..>3099)
                     /locus_tag="VFZ80_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6286937.1"
                     /translation="SQRFEKGNRDDMSERVLVADDDPDILTVVKVNLELDGFELDTAV
                     DGEDALQKATSNPPNVIILDIMMPRMDGLTALHRLRSQASTASIPIILLTARGLPEDR
                     VRGLELGADDYITKPFDITELAARVRAVLRRTQAARDLSPLTGLPGNFKITAEIENAI
                     KESREFALVHGDLDNFKAFNDHYGFMRGDEVIRFCGSCLTEAATSLGIDNAFVGHIGG
                     DDFVAMVPPSVAESFCKEVIERFDDGILDLYDTADALRGYIEVVDRRGERYAFPVVSL
                     SLGVASTDVRDIGTQWEASAIAVEMKEFAKKQPGSTYRIDRRTG"
BASE COUNT          528 a          999 c          976 g          596 t
ORIGIN      
        1 gtcgtcgttc atgacctcgg cgaggacggc ggcggggtac ttcccggcca gctctgcgag
       61 gtcgacggcg gcctcggtgt ggcctgccct cttcaagacc cctcccggct cgtatcggag
      121 cggatacatg tggccgggcc gagccagatc ctcgggtttg gtctcagggt cgatcacggc
      181 gagcaccgtg cgacagcggt ccgccgccga gatcccggtc gtggtgccgt gtgctgcgtc
      241 gatcgagatg gtgaacgcgg tcctgcggac gtcggtgttc ttggccacca tcatcggaag
      301 ctccagctcg tcgagacgct cccctacgac ggggaggcag atgatgccgc tggtgtaacg
      361 gagcatgaag ccgatgttgt ccggtgtggc atcctcggca gcgacgatca ggtcgccctc
      421 gttctcgcgg tcctcgtcgt cgaccatgat caccatctcg ccacgtccga tggcggcgac
      481 gatgtcttcg ataggtgcga atctcatctt ctgtccagga gccgttcgac gtatttggcc
      541 agcacgtcca tctcaaggtt gaccgtgtct ccctcgccgc gcaagcccag tgtggtgact
      601 tcgagagtgt gaggtatcag cgcgactgtg aaagtgttct cactcaccga ggcgactgtg
      661 aggctgaccc cgtcgaccgt caccgatcct ttctccacga gatagcgaag catgtcggga
      721 gaaaccgcca cttccatgat cacggacccg tcgtctgatg accgaattgc ctggatcgtc
      781 ccggttccgt cgacatggcc ctggacgatg tgcccgtcga accgaccact ggccggcatg
      841 ggtcgctcca ggttgacctg ggaccctttc tcgagccttc ccaggttggt tcggcccagc
      901 gtttccggga tgacatcgag actgaccgta tctccgcgcg gcgcgattgc ggtgagacag
      961 accccgttca ccgcaagtga ggcaccgacc cgcaactcac ccagcccggc gccacgaaga
     1021 acgactcgcc ggccgcttgc ggtttcctcc agcgatacga cctccccgat ttgttcgacg
     1081 attcctgtga acactcaacc atccaacgcg aagtcgaccc gtaggtcggg tccgagctta
     1141 cggacgtcga tgattttcgc ggattctgcc tctccgatgg agccgaatct gccgcccaga
     1201 ggagacctgc ctacccctcc gccgatgcgc cccccgatgt agacgactcc ccgctggacc
     1261 actcctgctc tcatccaggc tccggccagg gtcgggccac cctcgaggag aaccgacagg
     1321 aacccggcat cggccaaggc acgacaaacg gtctcgggac cgggccggcc gggcccgtcg
     1381 acgacgagga ggtcacccga gggaatggac atatcccccg gcgccacgac caccgggttt
     1441 cgctcccaga tcgctcgatc atgggggagc tcgctctccc cggccacgat caccggtctg
     1501 ggctgggggc cttggtagtc gggcagtctc acgtccaagc gaggatcgtc ttcgaccaac
     1561 gtccccgtgc ccacgatgac ggcgtcgttg gcggcacgaa gcgaatgggc atcgttcctc
     1621 gcctcctcag atgtgatcca ctgggaggtt cggtcgaggg cggcaaccga accgtcgagc
     1681 gtcatcgcgt atttcaaggt caccaggggc attccgcgct cgcggtgatg gaagtaggcg
     1741 gggtcgacct cgcgcgctcc cgggtcgtcg accaccagga catcgattcc tgcctggcga
     1801 agagactcca cgcctgcccc cgacactcgg gggtcagggt cgagagcacc gatgactacc
     1861 cgccccaccc cctcgtcgat gagacgatcg acgcacggcg gtgtcagccc gtgatgtacg
     1921 cacggctcca gggagacgta cacggtggat ccgaggacat tcgaggcgcg ctccagagct
     1981 aatgcctcgg catggggtga gcccgggcct tcgtgggctc cttcgccaac gatctcgccg
     2041 tgcctgtcga ccacgatggc gccgactcgt gggtttgggt gtgggtgttg ggtgcgagcc
     2101 aggtcgtagg ttcgctgcag aaaccgactc tcggtcaccc cgtgcgccgg tcgatgcggt
     2161 aggtcgagcc gggttgcttc ttggcgaact ctttcatctc tacggctatg gccgacgcct
     2221 cccattgtgt gccgatgtca cgcacgtcgg tcgacgctac tccgagggac agcgagacga
     2281 cggggaaggc gtagcgctca cccctgcgat cgacgacctc gatgtatccc ctgagagcgt
     2341 cggcggtgtc gtagaggtcg aggatcccgt cgtcgaacct ctcgatgacc tccttgcaga
     2401 aagactcggc gaccgacggg ggcaccatgg ccacgaagtc atcaccaccg atgtggccca
     2461 cgaatgcgtt gtcgatgccg agactcgtag cggcttctgt gaggcaactg ccacagaaac
     2521 ggatcacttc gtcgccccgc atgaacccgt agtgatcgtt gaacgccttg aagttgtcga
     2581 ggtcgccatg caccaatgcg aactcacgac tctctttgat ggcgttctcg atctcggccg
     2641 tgatcttgaa gtttccgggg agaccggtca gtggtgacag atcgcgagcg gcctgtgtgc
     2701 gtcggagcac ggcgcgaacc cgtgccgcca gctcggtgat gtcgaacggc ttggtgatgt
     2761 agtcgtcggc tccgagctcg agaccccgaa ctcgatcctc gggaaggcca cgtgccgtga
     2821 gcaggatgat cgggatgctc gcagtggagg cctggctgcg gaggcgatgt agggcggtga
     2881 ggccgtccat acgcggcatc atgatgtcga ggatgatcac gttcggcgga ttggacgtcg
     2941 ccttctggag cgcgtcctcg ccatcgaccg cggtatcgag ctcgaaccca tcgagctcga
     3001 ggttgacttt caccaccgtc aggatgtctg gatcgtcgtc tgcgaccaga actcgttcgc
     3061 tcatgtcgtc tctgttgccc ttctcaaagc gctgactgc
//
LOCUS       DASUTA010000282         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1765274, whole genome shotgun sequence.
ACCESSION   DASUTA010000282 DASUTA010000000
VERSION     DASUTA010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1765274"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..427
                     /locus_tag="VFZ80_05590"
     CDS             215..427
                     /locus_tag="VFZ80_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286938.1"
                     /translation="MTPAESVANLAQNLAEEGLVVKAALIYDVIDEAGERNLNFVVTE
                     NLATWDLQGMLTGILDVTGLRYRKPS"
     gene            473..1351
                     /locus_tag="VFZ80_05595"
     CDS             473..1351
                     /locus_tag="VFZ80_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007336356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286939.1"
                     /translation="MEGPIDSEASSSGESESGLNRNVQLTYFGLRLGIVLLVLMLFVS
                     VIHVITSENDWLRSLSSYYYTPARAVFVGSLCAIGAALIVYRGNTDTENVLLDFAGFL
                     AFIVAFVPTDLDESCGYCSGLAAEDVSVAITNNVVAVISVGFAAVVSGLFARRLLGLK
                     EDKQSRAARVSMWVSFVAIVLAGGYFALNTDQVEEYGHEVAAIFFFVFIILVVAVNAR
                     GLSQRREWTIDAVWAAAKNRYGVIAILMVLTLVVLFLIRNGFDHWILALEALLIGQFA
                     VFWGFQTEELRGSDTR"
     gene            1428..1877
                     /locus_tag="VFZ80_05600"
     CDS             1428..1877
                     /locus_tag="VFZ80_05600"
                     /inference="COORDINATES: protein motif:HMM:NF039728.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HEX6286940.1"
                     /translation="MSESATADSMGPVELSVLAFPETTFDGTISEALRDIVERGIVSI
                     LDLLIVAKDDEGVVSVIELADADAVIAAQFEKVDGEVMWLLSDQDIAAAGEFLEPGTT
                     AVLVVWESTWARQLKGAISRAGGRLVVHDRLDANAVAASIAASPGGG"
     gene            1874..2104
                     /locus_tag="VFZ80_05605"
     CDS             1874..2104
                     /locus_tag="VFZ80_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX6286941.1"
                     /translation="MMLIRPPVVREMRHDLWVQMLGRSDGSTQGDRAREGPGEPDAVA
                     VRLANLKQLGELRDSGVLTEEEFAKEKARLLG"
     gene            complement(2207..>2656)
                     /locus_tag="VFZ80_05610"
     CDS             complement(2207..>2656)
                     /locus_tag="VFZ80_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6286942.1"
                     /translation="GADRETERRDSEDEKQADQRQRPYLAGSNSEVEFDLGDRADPRG
                     WRCRHHDCAQVFEAERVPDELSGGERQRVAVTSSLIHEPSVLFADEPTGALDSLSAER
                     LLELVVGIAEESSLTLLIVTHDPRVAAYGQREVVVRDGRTSARSMTG"
BASE COUNT          457 a          793 c          875 g          531 t
ORIGIN      
        1 actggcaggc ggaatccctg cgagcccgtg ggctcgacgc gaccgttgtc gccgacgccc
       61 tggccggccc gcttgccgat ctgtccgagg agggaagtgc cacccttgct cgggcccggg
      121 agatccgcgc cggctgagct ggcgcactca ggctccggac atcgcggagc gccaatgccg
      181 ggccgtcggg taagcggtat ttaccatcat cgtggtgaca cccgcagagt cggtggccaa
      241 cctggcgcag aacctcgccg aggagggctt ggtagtgaag gctgctctca tctatgacgt
      301 gatcgacgag gcaggggaga ggaacctgaa cttcgtggtc actgagaacc tggctacctg
      361 ggacctccag gggatgctca ccggcatcct cgatgtgacc ggtttgagat accgcaaacc
      421 cagctgatca ttcgtcccgg cccgaaggcg gtgagctgta ggctcgtcgg ccatggaggg
      481 accgatcgat tcggaggcgt catcctcggg agagagcgaa tccggtctca acagaaacgt
      541 ccaactgact tacttcggtc tgcgtctggg catcgtcctc ctggtgctga tgctcttcgt
      601 ctcggtcatt catgtgatca cctcggagaa cgattggctg aggtcgttga gctcgtacta
      661 ctacacgccg gcacgtgccg tcttcgtcgg ctcgctgtgt gccatcggag cagcgctgat
      721 cgtctaccgg gggaacaccg acaccgagaa tgtgctgctc gacttcgccg ggttccttgc
      781 cttcatcgtc gccttcgtcc ccaccgacct cgacgaaagc tgtggctact gcagcggatt
      841 ggctgccgag gacgtctctg tcgccatcac gaacaatgtg gtcgccgtca tctcggtggg
      901 gttcgccgcc gtcgtatccg gactgtttgc gcgccgactg ctcggtctga aagaagacaa
      961 gcagtcccgt gccgctcggg tgtcgatgtg ggtgtccttc gtggccatcg ttctcgccgg
     1021 cgggtacttc gccttgaaca ccgaccaggt cgaggagtat ggccatgagg tcgccgccat
     1081 cttcttcttc gtgttcatca tcctcgtggt tgcggtcaat gcccgaggcc tctcgcaacg
     1141 tcgggaatgg accatcgacg ccgtttgggc cgccgccaag aatcgttacg gtgtcatcgc
     1201 catcctgatg gttctcacgt tggtcgtcct gttcctgatc agaaacgggt tcgaccactg
     1261 gatcctggct ctcgaagctc tcctcatagg ccagttcgcc gtgttctggg gatttcagac
     1321 agaagagctg cggggcagtg atacccgtta gagaagctgg agtggataac cgttagagaa
     1381 gctggagtgt cggaagtccg tttggtgtcg ttatggttcc cgcctcgatg agtgagagtg
     1441 caaccgcgga ttcgatgggg cctgtcgagc tcagcgtgct ggcgttcccc gagacgacat
     1501 tcgacgggac gatctccgag gctctccgcg acatcgtgga acgggggatc gtttccatcc
     1561 tcgatctcct gatcgttgcc aaggacgatg agggtgtggt atcggtgatc gagctggccg
     1621 acgccgacgc agtcatcgcc gcccaattcg agaaggtcga cggagaagtg atgtggctgc
     1681 tcagcgatca ggacatcgct gccgccggcg agtttctcga gcccggaacc actgcggtac
     1741 tcgtcgtgtg ggagagcacc tgggcccgcc agctcaaggg agccatctcg cgggcagggg
     1801 gacggctggt ggtccacgat cgtctcgatg ccaatgcagt ggcggcgtcg atcgcggcat
     1861 ccccgggagg aggatgatgc tgatccgtcc tccggtggtc cgcgagatgc gtcatgacct
     1921 ctgggtccag atgctgggga ggtcagatgg ttccacgcag ggagacagag cccgagaagg
     1981 acctggcgaa ccggacgcgg tggcagtgcg actggccaat ctcaagcagc tcggtgagtt
     2041 gagagattcg ggagtcctca ccgaggagga attcgccaag gagaaggcaa gactgctcgg
     2101 ctgaacggcg tttgagcgtg tgcagatacg gtggccacgg cacggtatct gcacacgctg
     2161 gccgcccgtg tcacagcggg gcagtgacgt caccgtgatc cgaggatcag ccggtcatgc
     2221 tcctcgcaga cgtacgcccg tctctgacca ccacctcccg ttgaccgtag gcggcgacac
     2281 gtggatcgtg tgtgacgatg aggagagtca gtgacgactc ttcggcgatg cccacgacga
     2341 gctcgaggag ccgttccgca gacagagagt cgagggcgcc agttggctcg tcggcgaaca
     2401 gcaccgacgg ctcgtggatg agcgagcttg tcactgcaac ccgctgtcgc tctcctccgg
     2461 agagctcgtc cggaacccgc tcggcctcga agacctgcgc acagtcgtgg tggcgacagc
     2521 gccaaccgcg cgggtccgct cggtcgccga gatcgaattc gacttcgctg ttgctgcccg
     2581 cgaggtacgg gcgctggcgc tggtcggcct gtttctcgtc ttccgaatcg cggcgttcag
     2641 tctcgcggtc ggcacc
//
LOCUS       DASUTA010000283         2046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_469947, whole genome shotgun sequence.
ACCESSION   DASUTA010000283 DASUTA010000000
VERSION     DASUTA010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469947"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..812
                     /locus_tag="VFZ80_05615"
     CDS             72..812
                     /locus_tag="VFZ80_05615"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286943.1"
                     /translation="MAEIKVENLHKEFGVGEDRVVALEKVNMEVSGHVFVSIVGPSGC
                     GKSTLLNILSGIETPSSGTVTITQDNRDAEPGYVFQAARLLPWRTVIDNLLFVQKDRS
                     EETRARCQKYLEMVQLGDKADKYPGELSGGMQQRVGIARAFSIEPDVLFMDEPFSHLD
                     AITARTLRRELHEMWRQTRKTVLFVTHDVGEAVELSNRILVFAKGGRLEDDLQIDLPF
                     PRDPAEEEVAVAKAQVLRKFEELDLVAS"
     gene            903..1976
                     /locus_tag="VFZ80_05620"
     CDS             903..1976
                     /locus_tag="VFZ80_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6286944.1"
                     /translation="MRRRTRTWIALLTMLALVLAACGDDEEGGSDGTQDGGDTRTVIR
                     FAFAPDPVWDYLNDNGHLVEWEEENNIRIVTSTTWDEFTFFAGGHGDIVSMGTLEIPV
                     LEQETGIQTVTFGKYNALRDSMMTSPDSGFETIADIPPGSTLCVASPVSATGFWAVAA
                     NELHGLDFRVGGGDFELIVNDHFVNPQNLVENEDCVAGIFIPEAATSYMRTGDMVVMY
                     DGQSPFQLYNTFSGFESDEAHIMSNLFTATEEFYDANPEAAAAFLELWQRGIELWEEN
                     QAAIIETYPQHFAVESDEDIAFITEYMAGPNDWFVDSVYFTDEWVEAERAIWDFQTEL
                     NPDNANYVEAGFEQPRFEVIPAP"
BASE COUNT          411 a          648 c          647 g          340 t
ORIGIN      
        1 gggagagcct tctcgtcgcc gaggaccagg ccggcatcga ctcgacagtt tcgaaaggtg
       61 ggaatctgta gatggctgag atcaaggtcg agaacctgca caaggagttc ggggtcggcg
      121 aagaccgcgt cgtcgccctg gagaaggtca acatggaggt ctcgggacac gtcttcgtct
      181 ccatcgtcgg accgtcgggt tgcggcaagt cgaccttgct caacatcctc agcgggatcg
      241 agacccccag ctcgggaacc gtgaccatca cccaggacaa tcgggacgca gagccgggct
      301 atgtgttcca ggcggcgcgt ctccttccct ggcgaacggt gatcgacaac ctcctcttcg
      361 tccagaagga ccgcagcgag gagacacggg cccgctgtca gaagtacctg gagatggttc
      421 agctgggcga caaggcggac aaataccccg gtgagctctc cggagggatg caacagaggg
      481 tggggatagc ccgggcgttc tccatcgagc ccgacgtctt gttcatggac gagccgttca
      541 gccatctcga cgccatcacc gcccgaaccc tgcgccggga gctccacgag atgtggcgac
      601 agacgagaaa gaccgtcctc ttcgtcaccc acgacgtggg tgaggcggtc gagctctcca
      661 atcgcatcct ggtcttcgcc aagggaggtc gactcgagga cgatctccag atcgatctcc
      721 ccttcccccg cgacccggcc gaggaggagg tggcggtggc caaggcccag gtcctgcgca
      781 agttcgagga gctggacctg gtcgccagct gaggtcgccg atcctgtatg acaaccaagt
      841 cttgacctga tacgaaaagg ctcggaccac ggggcccgtg ccctgccaat ggaggagacc
      901 tgttgagacg acggacacga acgtggatag ccctgctcac gatgctcgcc ctggtattgg
      961 cggcatgtgg tgacgacgag gaagggggga gtgacggaac ccaagatggt ggcgacaccc
     1021 ggaccgtgat ccgcttcgcc ttcgcccccg atccggtctg ggactatctg aacgacaacg
     1081 gccatctggt cgagtgggaa gaggagaaca acatccggat cgtgaccagc accacctggg
     1141 acgagttcac gttcttcgcc ggcggccatg gcgacatcgt ctccatgggc acgctggaga
     1201 tcccggtcct ggagcaggag acgggcatcc agacggtcac cttcggaaag tacaacgccc
     1261 tgcgtgactc gatgatgacc agccccgaca gcggattcga gacgatcgcc gacatcccgc
     1321 cgggatccac cctgtgcgtg gcgagcccgg tgtcggcaac cgggttctgg gctgtcgcgg
     1381 ccaacgagtt gcatggcctc gacttccggg tcggcggcgg cgacttcgag ctgatcgtca
     1441 acgatcactt cgtcaacccc cagaacctgg tggagaacga ggactgtgtc gccgggatct
     1501 tcatccccga ggcggccacc agctacatgc gcaccggtga catggtggtc atgtacgacg
     1561 ggcagtcccc gttccagctc tacaacacct tctccgggtt cgagagcgac gaagcccaca
     1621 tcatgtccaa cctgttcacc gccaccgagg agttctacga cgccaacccc gaggctgcag
     1681 cggcattcct cgagctgtgg cagcgcggaa tcgagctgtg ggaggagaat caggccgcca
     1741 tcatcgagac ctatccccag cacttcgcag tcgagtcaga tgaggacatc gccttcatca
     1801 ccgagtacat ggccgggccc aacgactggt tcgtcgactc ggtgtacttc accgacgagt
     1861 gggtcgaagc ggagcgggcg atctgggatt tccagaccga gctcaacccg gacaacgcca
     1921 actacgtcga ggccgggttc gaacagcccc ggttcgaggt gataccggca ccctgagtgc
     1981 gtgtccccct gccctcgcag ggggacaccg ctagaccacg cccagcgccc ggcgcagctt
     2041 ggtcac
//
LOCUS       DASUTA010000284         2144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1507879, whole genome shotgun sequence.
ACCESSION   DASUTA010000284 DASUTA010000000
VERSION     DASUTA010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507879"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1206)
                     /locus_tag="VFZ80_05625"
     CDS             complement(190..1206)
                     /locus_tag="VFZ80_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX6286945.1"
                     /translation="MKAWQLEDTNGIDSYVLNDVPEPDPGRGEVRVDLRFSGLNHLDI
                     WTSMGLPAPKHFPHTTGADGAGVVEAIGEGVAGHEVGDEVIVDPSISCGQCVYCRNDD
                     IVYCSEFKILGEHVPGTFAEKIVVPAKNVVPKPSAVDWEVAGSFGLAGATALRMLEKA
                     RLGQGEKVLVVGVGGGVSASAMYLAMAFRADVYVTSRSQEKIEWAISEGAKGGFDSSA
                     EFGKEMAELGGADLVVDNVGPATLKQSMRAARPGGRIAICGATSGPKFELNLPVLWFK
                     QLELVGSSMGNHAQFARALNWIRIGKAKAPVARVFGFDQLPDALRFLDSGEQIGNVVL
                     AHDS"
     gene            1205..1675
                     /locus_tag="VFZ80_05630"
     CDS             1205..1675
                     /locus_tag="VFZ80_05630"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX6286946.1"
                     /translation="MAATVASDFPPRGESLPVEIPQDREVHTTSVKVRFYELDPYDHV
                     NHTNYFSYFETARIEYLSEMGFGLDKMKEQGWQLVVVEIEARFLIAARYGQELTIHTW
                     VEDAGRVKSIWRQTMLRGDDEVARIKVTAAFTDLEGRPRRLPTEFADRLEELAG"
     gene            complement(1672..>2144)
                     /locus_tag="VFZ80_05635"
     CDS             complement(1672..>2144)
                     /locus_tag="VFZ80_05635"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX6286947.1"
                     /translation="PNGMFAPFTIGETPIPYGQTVSGIEVPQDLEVAQEIPMVLNDAG
                     VIGLSLNGKSFPATQPYVVDEGDWLVYHYYNEGLQIHPMHQHQFPQLVFAKDGIPLDH
                     PYWADTVNVAPGERYSVLVHADRAGTWVWHCHILTHVENEEGMFGMVTALIVEP"
BASE COUNT          410 a          664 c          678 g          392 t
ORIGIN      
        1 ggctcgatat gccgacgagc acccgaaagc actggacaga ctggcgcggt tcatgctcga
       61 caatcccggc gacacacccg aagagcaggc tgagcgggtg gccgagagca tcccgatggt
      121 ccggctcccc aaatagcttt ctgtgaggat ttttggtcgc tatacgacca aaaatcctca
      181 cagaagcgtt caggagtcgt gagcgaggac gacgtttccg atctgctcac ccgagtcgag
      241 gaaacggagg gcgtcgggga gctggtcgaa accgaagacg cgtgccaccg gcgctttcgc
      301 cttgccgatt cggatccagt tgagggcccg ggcgaactgg gcgtggtttc ccatcgaaga
      361 gcccacgagc tctagctgtt tgaaccagag aacggggagg ttcagctcga acttgggacc
      421 gctggtggcc ccacagatgg cgattcgccc gcccggcctg gcggcccgca tcgattgctt
      481 caaagtcgcc ggcccgacgt tgtcgactac caggtcggcg ccaccgagct cggccatctc
      541 ctttccgaac tccgccgagg agtcgaaacc acctttggcg ccttcggaga tggcccactc
      601 gatcttctcc tgtgagcggg aggtgacata cacgtctgcc cgaaacgcca ttgccaggta
      661 catggctgaa gcggacacgc caccgccgac accgactacc agcactttct ccccttgccc
      721 caggcgcgcc ttctccaaca tgcgaagagc ggtggccccg gccagcccga atgacccggc
      781 gacttcccaa tcgacagctg agggtttggg taccacgttc ttggcgggca ccacgatctt
      841 ctcggcgaag gtccccggca cgtgctcacc gaggatcttg aactcggagc aatacacgat
      901 gtcgtcgttg cggcagtaca cgcattgacc gcaggagatg gacgggtcga ctatcacctc
      961 gtcgccgact tcgtgtccgg cgacaccttc tccgatggct tcgaccactc cggcgccgtc
     1021 ggcccctgtg gtatgaggaa agtgttttgg cgccgggagg cccatcgagg tccatatgtc
     1081 gaggtggttt agccccgaga atcgcagatc gacacgcacc tcaccccgtc ccgggtccgg
     1141 ctcgggaacg tcgttgagga cgtaggagtc gattccgttg gtgtcttcga gctgccaggc
     1201 cttcatggcg gcaacggtag cctcggactt cccgccccga ggagagagct tgccggtcga
     1261 aataccccag gacagggaag tccacacgac gagtgtcaag gtgcgcttct acgagctcga
     1321 cccctacgac cacgtcaacc acaccaacta cttctcgtac ttcgagacgg cgcggatcga
     1381 gtacctgtcc gagatggggt tcgggctcga caagatgaaa gagcagggct ggcagctggt
     1441 cgtcgtggag atcgaggcga ggttcctgat cgcggcgcgg tacggccagg agctcaccat
     1501 ccacacgtgg gtggaggatg ccggccgggt gaagtcgatc tggcgtcaga ccatgcttcg
     1561 tggcgacgac gaggtcgccc ggatcaaggt cacagccgca ttcaccgacc tcgagggcag
     1621 acctcgccgt ctgcccaccg agttcgccga ccgtctcgag gaactggccg gctagggctc
     1681 tacgatcagg gccgtgacca tgccgaacat cccttcctcg ttctcgacgt gtgtcaggat
     1741 gtggcagtgc cagacccaag tgccggcacg gtcggcatgg accagcaccg agtagcgctc
     1801 accgggagcg acattgacgg tgtcggccca gtaaggatgg tcgagtggga tgccgtcctt
     1861 ggcgaacacc agctggggga attgatgctg gtgcatcgga tggatctgta gtccttcgtt
     1921 gtagtagtgg tagacgagcc agtcaccctc gtcgaccaca tagggctgcg ttgccgggaa
     1981 ggacttgccg ttgaggctca acccgatgac acccgcgtcg ttgagcacca tcgggatctc
     2041 ctgggcgacc tccagatcct gggggacctc gatccctgag accgtttgcc cgtaagggat
     2101 gggcgtctcg ccgatggtga acggggcgaa catgccgttg ggga
//
LOCUS       DASUTA010000285         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_601854, whole genome shotgun sequence.
ACCESSION   DASUTA010000285 DASUTA010000000
VERSION     DASUTA010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601854"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VFZ80_05640"
     CDS             complement(<1..396)
                     /locus_tag="VFZ80_05640"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX6286948.1"
                     /translation="MRVILIGGGKVGSYLARELAKSGHVVTVIEESHTHAASLADHSK
                     VLVLEGDGTDIGLLESADAHRADWVLAVTGLDEVNLVACQLALTLGAHKVLARLNNPL
                     NRPTFDALDIPVVAVTDVMATLISREVEVA"
     gene            complement(393..1055)
                     /locus_tag="VFZ80_05645"
     CDS             complement(393..1055)
                     /locus_tag="VFZ80_05645"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX6286949.1"
                     /translation="MRVVIAGCGRVGSDLALTLSEEGHDVSVIDTREGVFDRLGSTFN
                     GTTHEGLGYDVRVLKAAGIEYADAFIAATDSDNANAMAVQVAERVFGVPKTIARLDDP
                     AREEAYRALGVEFVPGAKLTSKVIHEQIVREEFNFHVTFSGGDVEIVDMTVGPEGAGR
                     KVAELEIPDDLRVAAIYRGHRTFIPDSDFVLAEGDMVVAAARTGARRKIRRFLALTED
                     EG"
     gene            complement(1075..1485)
                     /locus_tag="VFZ80_05650"
     CDS             complement(1075..1485)
                     /locus_tag="VFZ80_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286950.1"
                     /translation="MTRDPSLPHDPRTSRRRLAFALALTVALTAPACGDENEPAGEWD
                     AIWRNTVATVAGATGGDLTPEQCQDLLGYLRVQRTVMTPVPLEDLQTPVDTWFNEAEG
                     IFFECDLDSDGAQDSLLTLEAVEGEVDIVLEVEQ"
     gene            1565..1948
                     /locus_tag="VFZ80_05655"
     CDS             1565..1948
                     /locus_tag="VFZ80_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6286951.1"
                     /translation="MTHEQVQSWLDDYVSAWASNDADRIAELFSEDAAYSYRPWRDDA
                     NTVIGRDEVVSSWQDDPDDPDTWEAEYAPYAVDGDRAVAVGRTRYFAHDDEPERVYHN
                     AFLLQFDDEGRCAEFAEFYVREKRA"
     gene            complement(1945..>2216)
                     /locus_tag="VFZ80_05660"
     CDS             complement(1945..>2216)
                     /locus_tag="VFZ80_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEX6286952.1"
                     /translation="VRSQALTLRQVFSQVALFAPDDYLAGSAGGNFILVAGDQAIDID
                     DIEERIDRRGGSERGIEGVELTAFIGEAQVLRDDFAPVDQMLGRP"
BASE COUNT          412 a          716 c          689 g          399 t
ORIGIN      
        1 ggccacctcc acctccctcg agatcaaagt ggccatcacg tcggtaacgg cgactaccgg
       61 gatgtcgagg gcatcgaagg taggacggtt gagtgggttg ttcagccttg cgagaacctt
      121 gtgggcgccc agcgtcaacg ccaactgaca tgcgaccaga ttgacttcgt cgagaccggt
      181 cacagcaagg acccaatcgg cacggtgcgc atcggccgac tcgagaagtc cgatatccgt
      241 cccgtcaccc tccaggacca ggaccttgga gtgatcggcc agactggcag cgtgggtgtg
      301 tgactcctcg atgacggtca cgacatgacc cgacttggcc agctcacgag cgaggtagga
      361 gccaaccttc ccgcctccga tcaagatgac tctcatccct cgtcctcggt cagggcgaga
      421 aagcgacgga tcttgcgccg ggcgccggtg cgggccgcgg ccaccaccat gtctccctcg
      481 gcgagaacga agtcactatc gggaatgaac gtccgatgcc cccggtagat cgccgcaact
      541 ctcaggtcgt cgggtatctc gagctctgcc acctttcgcc ctgccccctc cggccctacc
      601 gtcatgtcga cgatctcgac gtcgcctccg gagaaggtca cgtggaagtt gaactcctct
      661 cggacgatct gctcatgtat caccttcgag gtgagtttgg cgcccgggac gaactcgaca
      721 ccgagggcac gatacgcctc ctcccgggca ggatcatcga gacgggcgat ggtcttgggc
      781 acgccgaaga cccgctcggc gacctggacg gccatcgcgt tggcgttgtc cgagtcggtg
      841 gcggcgatga aagcgtcggc atactcgatg cccgccgcct tgagcacccg cacgtcgtag
      901 cccaggccct cgtgagtggt gccgttgaac gtggagccga gccggtcgaa gacaccttcg
      961 cgggtgtcga tgactgagac gtcgtgcccc tcttcggaca gggtgagggc caggtcactg
     1021 ccgaccctcc cacatcctgc gatcaccaca cgcattcggc acctgtctgt gtcatcactg
     1081 ttcgacctcg agcacgatgt cgacctcgcc ttccacggcc tcgagagtga gtagcgaatc
     1141 ctgggcgccg tccgaatcga ggtcgcactc gaagaagatc ccttcggcct cgttgaacca
     1201 ggtgtcgacc ggtgtctgca ggtcctcgag tggcaccggc gtcatgaccg tgcgctgcac
     1261 tcgcagatat ccgagtaggt cctggcactg ctcaggggtg aggtcgccac cggtggcccc
     1321 ggccactgtg gccacagtgt tcctccagat cgcatcccat tcaccggcgg gctcgttttc
     1381 atctccgcaa gccggggcag tgagcgcgac cgtgagagcg agggcgaatg cgagccgccg
     1441 cctggaggtt ctggggtcat gaggaagact tgggtctcga gtcaaacccg tcaaccctac
     1501 atcgactcgg gaggtgggtc gcttcactcg ggcgacgtct ggaaggcgtg cgtatcctcg
     1561 gatcatgact catgagcaag tccaatcatg gctcgacgac tatgtctcgg cctgggccag
     1621 caacgacgcc gaccgcatcg cagagctctt cagtgaagat gccgcctaca gctaccgacc
     1681 gtggcgtgac gacgccaaca cggtgatcgg gagggatgaa gtcgtctcca gctggcagga
     1741 cgatcccgac gatcccgaca cctgggaagc ggaatacgcc ccctatgcag tcgacggaga
     1801 cagggcggtg gcggtggggc ggacccgcta tttcgctcac gacgacgagc ccgagcgcgt
     1861 ctatcacaac gctttcctcc tccagttcga cgatgaggga cgatgcgccg aattcgccga
     1921 gttctacgtg cgggagaaga gggcctaggg ccgtcccagc atctgatcca cgggggcgaa
     1981 gtcgtccctg aggacctgtg cctctccgat gaaggcagtc agctcgacgc cttctatacc
     2041 tcgttccgat cccccacggc ggtcgatccg ctcctcgatg tcgtcgatgt cgatggcctg
     2101 gtcgccggca acgagaatga agttgccacc cgccgacccg gctagatagt cgtccggagc
     2161 gaagagggcg acctggctga acacctggcg cagagtgagc gcctgtgatc tcacga
//
LOCUS       DASUTA010000286         3938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1249313, whole genome shotgun sequence.
ACCESSION   DASUTA010000286 DASUTA010000000
VERSION     DASUTA010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249313"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1912
                     /locus_tag="VFZ80_05665"
     CDS             188..1912
                     /locus_tag="VFZ80_05665"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015622793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX6286953.1"
                     /translation="MRWSQAFIPTLREPPADSEAVSHKLLVRGGFIRQLAAGSYTMLP
                     LGQKVRLKIAQIIREEMNAIGGQELMLPTLHPAEIWRKSGRYELMGDALFKLQDRKGA
                     DLALGMTEEEIFGMLASELFSYKQLPQIWYQMHTKFRDEPRPKSGLLRIREFTMKDSY
                     TLDIDLPGLDRGFDRHHDAYVRIFARMGLDAVDVEASSGAMGGTESIEFIQRSEAGED
                     WVVTCESCDYRANVERAVSALEPIDDGETTELERFPTPGLRTIRALAEAHPDIASPRR
                     QIKSLVYIVEGEPVLVLLRGDHDLQEQKLRDATGAPEDGARPAKPEEIRELLGADPGS
                     LGAVGVDDIEIVADRALEGRVNMTTGANEDDWHYKGVDVSRDIDVKTWGDLREVTDGE
                     RCVKDGGRLELWKGIEVGHIFKLGTQYSEAFGAYVQDEAGASHPIIMGSYGIGLERDM
                     AAVVEASHDEKGIVWPVSIAPYEVVVTVIRPDDEATMAAANRLYDDFDSAGVDVLLDD
                     RDERPGVKFADSELIGIPYRVTVGPKGVESGVAEITERNGMAKSEMPFGEVVASLTER
                     IEAARFGI"
     gene            2032..2502
                     /gene="rimP"
                     /locus_tag="VFZ80_05670"
     CDS             2032..2502
                     /gene="rimP"
                     /locus_tag="VFZ80_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX6286954.1"
                     /translation="MTQIADIWSVVEPYLAEERLELDDLELSGRGRGRILRVTVDGDD
                     VNVDRLAELSRGLSRLLDDEPALQDAYQLEVSSPGLERKLRRPSHFRKSLGREVVVKT
                     TVDDHKATYRGTLTTAGENTFTVDSEQGVTEVPYDDVISAKTVFRWEKAPKPGH"
     gene            2511..3704
                     /gene="nusA"
                     /locus_tag="VFZ80_05675"
     CDS             2511..3704
                     /gene="nusA"
                     /locus_tag="VFZ80_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX6286955.1"
                     /translation="MQLDSMMEAIEVLARERGVEVDAILDAFANALVAAYKRSPGAAE
                     EARVTIDPESGEVTVYAQELDEDGNVIDEWEDTPEGEAFGRIAAQSFKQVMQFKLREA
                     KREKVFEMYEGREGDLVTGIVQQVDYRSVILDLGDAEAILPGSERIPFERLDRGNRVK
                     ALILEVRNETKGSQIVVSRSHPDLVRRLLELEVPELTDGTVEIVNIAREPGHRTKIAV
                     ISHDPNVDPKGACVGARGSRVRQVVNELRGEKVDVVQWREDVSQFIAEALGPAKVKEV
                     HIDEEEKTADVIVSEHQLSLAIGKEGQNARLAARLSGYKVDIRSDTEEVAEPVAEETD
                     ESAETAGDAGEVGASGVAGETDAEVAAAAAEEEQAQVDVEEKPEASLPEVGQTEESET
                     DTESE"
     gene            3712..>3938
                     /locus_tag="VFZ80_05680"
     CDS             3712..>3938
                     /locus_tag="VFZ80_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006906268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEX6286956.1"
                     /translation="MRVYELARELGVESKDVLSLAEELGLDLKTASSGMDDDAAELIR
                     LAFAPEEDTTETAEPEAEEAAEEETAEPEVV"
BASE COUNT          812 a         1179 c         1309 g          638 t
ORIGIN      
        1 ggcacctcga aaggtgcagt cgggcatgct gaccgtgagg atcaccggtt ccacctgacc
       61 acatgcggcg agagcaagca tcgccatcaa ggtcacgacc ggggcgatcg agcgcatcgt
      121 gtcaacgtaa tgcagcacct aaatcgatcg gggtaaaggg agactccatg actaccgtcc
      181 tccacccatg cgttggtcac aagcgttcat acccaccttg cgggagccac cggcggactc
      241 agaggccgtc agccacaagc tcctggttcg aggcgggttc atccgccagc tggcggcagg
      301 gtcttacacg atgctgccac tcggtcagaa ggtccgtctc aagatcgccc agatcatccg
      361 ggaggagatg aacgccatcg gcggccagga gctcatgctg cccaccctgc atccggccga
      421 gatctggagg aagtccggcc gctacgaatt gatgggggac gctctcttca agctccagga
      481 tcgcaaggga gccgacctcg ccctgggcat gaccgaggag gagatcttcg ggatgctcgc
      541 aagcgagctc ttctcctaca agcaacttcc gcagatctgg taccagatgc acaccaagtt
      601 cagggacgag ccccgtccca agtcgggcct gctgcggatt cgggaattca ccatgaagga
      661 ctcctacacc ctcgatatcg acctgcccgg tctggatcgt ggtttcgaca gacaccacga
      721 cgcatacgtc cgcatcttcg ctcgcatggg tctcgacgca gtcgatgtcg aagcctcatc
      781 cggtgcgatg ggcgggacgg aatcgatcga gttcatccag cggagcgagg ccggtgaaga
      841 ctgggtggtc acgtgtgaat cgtgtgacta tcgcgccaac gtcgagcgag cggtttccgc
      901 cctcgaaccg atcgacgacg gcgagaccac agagctggag cgattcccca cgccagggtt
      961 gcgcacgata agggccctcg ccgaggctca ccccgatatc gcatcccctc ggcggcagat
     1021 caaatccctg gtctacatcg tcgaaggaga gccagtgttg gtcctgctgc gcggtgatca
     1081 cgatctccag gagcagaagc tgcgcgatgc gaccggtgca cctgaagacg gagcccgccc
     1141 cgccaagccg gaggagatcc gtgagttgct gggggcggac cccggcagtc tcggagccgt
     1201 tggcgtggac gacatcgaga tcgtcgcgga tcgtgccctc gagggccggg tcaacatgac
     1261 caccggggcc aacgaggacg attggcatta caagggtgtc gacgtgagcc gtgacatcga
     1321 cgtcaagaca tggggcgatc tacgtgaagt caccgacgga gagcgatgcg tgaaagatgg
     1381 cggacgactc gagctgtgga agggcatcga ggtgggtcat atcttcaagc tgggcaccca
     1441 gtattccgag gcgttcgggg cctacgtcca ggacgaggca ggagccagcc accccatcat
     1501 catgggctcc tacggcatcg gtctcgagcg agacatggcg gctgtcgttg aagcctctca
     1561 cgacgagaaa ggcatcgtct ggcccgtctc gatagccccc tatgaagtcg tcgtcaccgt
     1621 catccgaccc gacgacgagg ccaccatggc cgctgccaac aggctctacg acgacttcga
     1681 ctccgccggt gtcgacgttc tcctcgacga ccgcgacgag cggcccggcg tcaagttcgc
     1741 cgactccgag ttgatcggca tcccctatcg ggtgacggtg gggcccaaag gggtggagtc
     1801 cggggttgct gagatcaccg agcgcaacgg gatggcgaag agcgagatgc ccttcggtga
     1861 ggtggtggcg agtctgacag agcggatcga agcggccaga ttcggcattt agcctggcta
     1921 tcggcatcac ctataatcgc ggtctacaac agggacgctt ttcggtcagg tgggcgcctt
     1981 tgggcaccca cctctttgtt gtggagtgac tgaccgaata ggtgattgcg agtgacgcag
     2041 atagctgaca tttggagcgt ggtagagccc tacctcgccg aggaaagact cgaactggac
     2101 gatctcgagt tgtccggccg cggtcgcgga cgaatccttc gcgtcacagt cgacggggac
     2161 gatgtgaatg tcgatcgtct cgccgagctc tctcgagggc tctcccgcct gctcgacgac
     2221 gagcccgcgc tgcaggacgc gtatcagctg gaggtcagct cccccggact cgagcgcaag
     2281 ctgcgccgcc cgtcccattt ccgcaagtcg ctgggccgtg aggtggtcgt caagaccacg
     2341 gtcgacgatc acaaggccac ctaccgcgga accctgacca ccgccggtga gaacaccttc
     2401 accgtcgatt ccgagcaggg cgtcaccgaa gtcccctacg acgacgtaat cagcgccaag
     2461 acggtcttcc ggtgggagaa ggcgccgaag ccaggccact aaggagagag atgcaactcg
     2521 acagcatgat ggaggcgatc gaggttctcg cccgggagcg aggcgtcgaa gtagacgcga
     2581 tcctcgatgc gttcgccaac gccctggtgg cggcctacaa gcggagcccg ggcgccgccg
     2641 aggaggcacg cgtcaccatc gaccccgaat cgggcgaggt gaccgtatac gcacaggagc
     2701 tcgatgagga cggcaacgtc atcgacgagt gggaggacac gcccgaggga gaggccttcg
     2761 gtcgcatcgc cgcgcagtcg ttcaagcagg tgatgcagtt caagctgcgt gaggcgaaac
     2821 gggagaaggt cttcgagatg tacgagggtc gcgaggggga ccttgtgacc gggatcgtgc
     2881 aacaagtcga ctatcgatcg gtgatcctcg atctgggcga cgccgaggcc atactccccg
     2941 gcagcgaacg catccctttc gagcggttgg accggggcaa tcgggtcaag gcgctcatcc
     3001 tcgaggtgcg caacgagacc aagggctccc agatcgtggt ctcccgaagc caccccgacc
     3061 tggtgcgccg cctcctcgag ttggaggtcc ccgagttgac cgacgggacg gtggagatcg
     3121 tcaacatcgc tcgtgagccc ggtcatcgca ccaagatcgc cgtcatctct cacgacccca
     3181 atgtggatcc caagggtgcg tgtgtgggag cccgcggatc gagggtccgc caggtcgtca
     3241 acgagctacg tggcgaaaag gtcgacgtgg tgcagtggcg tgaggacgtc tcgcagttca
     3301 tcgccgaggc cctcggccct gccaaggtga aagaggtcca tatcgacgag gaggagaaga
     3361 ccgccgacgt gatcgtctcc gagcatcagc tctctctcgc catcggcaag gaaggccaga
     3421 acgccagatt ggccgcccgg ctgtcggggt acaaggtcga catccgatcc gacaccgagg
     3481 aagtcgcaga gcccgtggcg gaggagaccg acgagagcgc ggagacggct ggagatgccg
     3541 gggaagtcgg tgcgagtggg gttgctggag agactgatgc agaggtggcc gccgcggccg
     3601 ccgaagaaga gcaagcacag gtcgacgtcg aggagaagcc tgaggcgtcc ctacccgaag
     3661 tggggcaaac tgaagaatct gagacagaca ccgagagtga gtgattgctg aatgagggtt
     3721 tacgagctcg cccgtgagtt gggcgttgag tccaaggacg tgttgtcgct ggccgaggag
     3781 ctcggcctcg acctgaagac ggcgtcctcg ggcatggacg acgacgcagc agagctgatc
     3841 cgtctcgcct tcgcaccgga ggaggacacg accgagaccg cggaacccga ggccgaggag
     3901 gcagcggagg aggaaactgc cgagcccgag gtcgttga
//
LOCUS       DASUTA010000287         3205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_602069, whole genome shotgun sequence.
ACCESSION   DASUTA010000287 DASUTA010000000
VERSION     DASUTA010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602069"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VFZ80_05685"
     CDS             complement(<1..465)
                     /locus_tag="VFZ80_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286957.1"
                     /translation="MVFVIVAAAGVLLVALYHLVAGWLFSNGLKRETFVVGPRAKDLG
                     VWVRAVGADRVELESKQPRQDIGHPGTLGMRWEGGYARVGDVIAASNGRIVRKFVPGP
                     DGVPPVCSGDLDECEPIELDGFAYPHDPHDAGLEFSEVEYESPLGSMGAWLVP"
     gene            complement(492..1133)
                     /locus_tag="VFZ80_05690"
     CDS             complement(492..1133)
                     /locus_tag="VFZ80_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6286958.1"
                     /translation="MTHRIFLVDDHDVVRAGMKSVLDASFDIVGEAADVNEAVEGILR
                     TEPEMVVLDVKLPDGSGADVIERVRRIRPEVKFMALTVSTSRDDVVRLMQAGVDGYIT
                     KTTASDELPHLIDQTLEGLRPVSPDVAAYMLDIDEDIEAGSTIQRLTPREREVVNLIA
                     RGYSYRETAARLSISVKTLESHMGHIFEKLSVASRHELAALAYEEGYVDPPAV"
     gene            complement(1135..2253)
                     /locus_tag="VFZ80_05695"
     CDS             complement(1135..2253)
                     /locus_tag="VFZ80_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6286959.1"
                     /translation="MLRGSFTKRGSPELETTLRRMVVVFRFLGWIWMVLLVVVTVLAD
                     PPPRLWIVYAALGLATLWTLLTWYMARSHPKQLASGGWFAIDTLVMLAIGAASVAAGA
                     TELFHGGLPLSYVFVGALWGGLPGSLIAAILLAAEQFAVHVIAELGAVRAAGSIIFFI
                     VAAIVGWTFDRLRDYDLARQRAEGALAAEREAVAAHEARVTLVNKLHDSVLQTFHAIR
                     MGADDPAQSRYLARRQERELRRNIEEWRSQYKHSFRAAMLAVRDDVEDIHRVEIEAVI
                     RDDAELTPELEAGIEAAREALTNAVKHSGANRISVFSELVDGTAQIHVRDDGRGINGS
                     DIGRLSTRLNQRVESVGGLVTVDSGNSDGTEVKITVGV"
     gene            complement(2464..3009)
                     /locus_tag="VFZ80_05700"
     CDS             complement(2464..3009)
                     /locus_tag="VFZ80_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286960.1"
                     /translation="MIGSLAWGLYSYGELQDRLDELPRTLVPGVVDVEVDEPQTLTVF
                     YEDPASSGTFAVQSGGSNTLTAPPVELAVSSPSGQEVDVLPYERDLRFDHEGRVLTAI
                     STFSADSAGIYIVEVEGDVPASARVSVGRVVESSLVATVIGVVGSFVLSVIGLLIAVV
                     ALSIRRATGVATPGREPLVRV"
BASE COUNT          589 a         1025 c         1010 g          581 t
ORIGIN      
        1 gggcaccagc caggcgccca tcgagcccag tggtgactcg tattccactt cggagaactc
       61 gaggcccgcg tcgtgcgggt cgtggggata ggcaaagccg tcgagttcga tcggctcaca
      121 ctcgtccagg tccccgctgc agaccggtgg cacgccgtcg ggccccggca cgaacttccg
      181 gacgattcgt ccattggagg cggcgatgac atcaccgacc cgggcatagc ccccctccca
      241 acgcatgccc agggttccgg gatgcccgat gtcctggcgc ggctgcttcg actcgagctc
      301 gacccggtcc gccccaacgg cgcgaaccca cacgccgaga tcctttgctc ttggacccac
      361 gacgaaggtc tctctcttga gtccgttgga gaagagccaa ccggcaacca ggtgatacag
      421 agcgaccaga aggacgccgg cggcagccac gatgacgaag accacgtcgc cagtttggcc
      481 gttttctcag gtcagacggc gggagggtct acgtagccct cctcgtacgc gagggcggcc
      541 agctcgtgtc ggctggccac cgacagcttt tcgaagatgt gacccatatg gctctccagt
      601 gtcttcaccg agatgctgag cctggccgcc gtctcccggt aggagtagcc gcgggcgatc
      661 aggttgacca cttcccgctc tctcggggtc agcctctgga tggtcgagcc ggcctcgatg
      721 tcctcgtcga tgtccagcat gtaggcagcc acgtcggggg acaccggccg cagaccttcg
      781 agggtctggt cgatcaggtg cggaagctcg tcgctggccg ttgtcttggt gatgtagccg
      841 tcgactccgg cctgcatgag ccggacgacg tcgtcgcgtg aggtcgagac cgtgagcgcc
      901 atgaacttga cctcgggcct gattctgcgc actcgctcga ttacgtctgc gccgcttccg
      961 tcgggaagct tcacatcgag cacgaccatc tccggctcgg tgcggagaat gccttcgact
     1021 gcctcgttga cgtcggccgc ctcaccgacg atgtcgaatg acgcgtccag gaccgacttc
     1081 atgccggcgc ggaccacatc gtggtcgtcg acgaggaaga tgcggtgtgt catttcagac
     1141 tccaacggtg atcttgactt cggtcccatc ggaattgcca ctgtcgactg tcaccagccc
     1201 accgaccgac tcgacgcgct ggttgagccg ggtggagagc cttccgatgt ctgacccgtt
     1261 gatcccacgg ccgtcgtctc tgacgtgaat ctgggcggtg ccatcgacca gctcggagaa
     1321 gaccgagatg cgatttgccc ccgaatgctt gaccgcgttc gtcagcgcct ctcgagcggc
     1381 ttcgatgccg gcctccagct ccggtgtcag ctccgcgtcg tcacggatca cggcttcgat
     1441 ctcgactcgg tggatgtcct cgacgtcgtc gcgaaccgcc aacatcgcgg cacggaacga
     1501 gtgcttgtac tgcgagcgcc actcttcgat gttgcgtcgc agctcacgct cctggcgacg
     1561 tgccagatag cgtgactggg ccggatcgtc ggctcccatg cggatagcat ggaaagtctg
     1621 gagcaccgag tcgtgcagct tgttgaccag ggtcactcgg gcttcgtggg cggcgacagc
     1681 ctcgcgctcg gcggcaaggg cgccttccgc cctttgacgc gccaggtcgt agtcacggag
     1741 acggtcgaag gtccatccga cgatggcggc gacgatgaag aagatgatgc tgcctgccgc
     1801 ccgaaccgct cccagttcgg cgatgacatg gaccgcgaac tgctcggcgg caaggaggat
     1861 ggcggcgatc agtgagccgg gcagaccccc ccacagcgct ccgacgaaca catatgacag
     1921 aggcaggccc ccatggaaga gctcggtggc acccgctgcg accgatgctg cgccgatggc
     1981 cagcatgacc agcgtgtcaa tcgcgaacca ccctccgctc gccagctgct tggggtggct
     2041 tcgggccatg taccaggtga ggagagtcca gagagtggcc aaacctaacg cggcgtagac
     2101 gatccacagg cgcggtggcg ggtcggcgag aaccgtcacc accacgagca ggaccatcca
     2161 gatccagccg agaaagcgga acaccacgac catgcgtcgc agggtcgtct cgagctcggg
     2221 gctgccccgc ttggtgaaac tgccgcgcag catggtgagg aaggtagttg aaggtctgat
     2281 gcgaagttct ccagaacggg cctgaagggc tgcagatgca gcccttcagg tgaggtcccc
     2341 attgtcgctt cgagggacgt atcggttgcc ctgaaggtcc ccctatgtga cccatcatgt
     2401 atcgcccatg cctgtctgta tatagggaat ttcccctatc aactggatgg gtgggggtcg
     2461 tgtctacacg cgtaccagcg gttctcgtcc tggcgtcgcg acaccagtcg cacgccggat
     2521 cgacaacgcg accacggcaa tcagcaaacc aatcaccgac agtacgaacg agccaaccac
     2581 acctatgacg gtggcgacca ggctgctttc cacgacacgg ccgaccgata ctcgggccga
     2641 tgccggcacg tcaccctcca cttccacgat gtagatgccc gcggagtctg ctgagaatgt
     2701 cgagatcgca gtgagaaccc gtccctcatg gtcgaaacga agatcacgct cgtagggcag
     2761 tacgtctact tcctggcccg agggtgagct cacggccagt tcaaccgggg gagcggtgag
     2821 cgtattcgag ccaccgcttt ggacggcgaa cgtgccgctc gacgctgggt cctcgtagaa
     2881 cactgtcaat gtctgcggtt catcgacctc gacatcgacg acgcccggca cgagggtcct
     2941 gggcagctcg tccaatcggt cctggagctc tccgtagctg tagaggcccc aggcgaggga
     3001 tccgatcatc acaacgacgg agatccagag aacggcccag ggccatgacg actttcgact
     3061 tcgaattggc attctcgtgc cctccttggg tcacagcgat aggaacctgc caatgagctg
     3121 gttggcgagc tcgggttggg ccaaagccgt gatgtggtgt gcatcgggga ctcgggccac
     3181 ctcgaggttg ggcatgagcc gtcgc
//
LOCUS       DASUTA010000288         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1379826, whole genome shotgun sequence.
ACCESSION   DASUTA010000288 DASUTA010000000
VERSION     DASUTA010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379826"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFZ80_05705"
     CDS             complement(<1..270)
                     /locus_tag="VFZ80_05705"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6286961.1"
                     /translation="MSQLKELLRIRDFRYLWLAQILSDFGDNLTFLTLLILIQRLTGS
                     TVALAGLMVAIALPTLLFGTLAGVYVDRIDRKKAMMVSDLLRAIVV"
     gene            complement(270..863)
                     /locus_tag="VFZ80_05710"
     CDS             complement(270..863)
                     /locus_tag="VFZ80_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6286962.1"
                     /translation="MSEQAEFMEIEDIEVFEVLNNTTRFRIIRYLEEPRSIREVAELL
                     DVPTTRLYYHFNLLEEAGIISVVETRKAGAMLQKVYRSNAKGFRPSPKVAQGDHEPHE
                     LAKITAGVVLDGARVDAEEGLTEHFARVRSGDDEAKLPGSIGRSLTFMTEEQAKTFTE
                     KLQKLVEEEFDTEEEGDGRPYGFTFVFFPLAGSGESD"
     gene            complement(863..1096)
                     /locus_tag="VFZ80_05715"
     CDS             complement(863..1096)
                     /locus_tag="VFZ80_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286963.1"
                     /translation="MIAAAPRRRVIEGAEERVMYRETVHTARYIQEERQRQIQPRRRQ
                     ERHSFAPMLGRVLVDMGERLQRGTSVSKPVTEV"
     gene            1184..1771
                     /locus_tag="VFZ80_05720"
     CDS             1184..1771
                     /locus_tag="VFZ80_05720"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286964.1"
                     /translation="MRLGIDLDGVVANFTKGWMSFYNEQFGTALAVEDSKRWHDLVEL
                     THFDHIGEFWEWSADLDGRSVFWHLEPFPGAVEALRSLADAGHHVIVVTTKPDFAVGD
                     TYEWVERHRIPAAEIHILEDKWLVECDVYLDDGPHILPGLVEHRPDSTVCRYVRPWND
                     PVPGAIDVNDFVEFREVVDQLAGGEAAPLASPHDL"
     gene            1844..>2388
                     /locus_tag="VFZ80_05725"
     CDS             1844..>2388
                     /locus_tag="VFZ80_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX6286965.1"
                     /translation="MNRRALWALIAVLALVVAACGDGEGGDDTTTTAAPGTTAPDETT
                     TTEDMTTTTGGEMSDWPDTLVFGFVPSQEATELQDDVDTFAAILSDALGITVEGTVTT
                     DYTALALAMGTGQADMGAFGPAGYVIASREFDNLELLAQSVRFGDSTYHAQYFTNDPS
                     ICGDAGTEEGAFYYDDSGAVVP"
BASE COUNT          462 a          748 c          723 g          455 t
ORIGIN      
        1 gaccacgatg gcccgcagga gatcggagac catcatcgcc ttcttacggt cgatgcgatc
       61 gacgtagacc ccggcgaggg ttccgaagag aagagtgggg agggcgatgg ccaccatgag
      121 cccggccagc gccaccgtgg agcccgtcag ccgttgaatg aggatgagga gcgtgagaaa
      181 ggtgaggttg tcgccgaagt cggagaggat ctgggccaac cagagataac ggaagtcccg
      241 aatgcgcagg agctctttga gctggctcat cagtcgctct cgccgctgcc ggcgagtggg
      301 aagaagacga aagtgaagcc atagggccgg ccatcgccct cttcctcggt gtcgaactcc
      361 tcctcgacca gcttctgcaa cttctcggtg aatgtcttcg cctgttcctc ggtcatgaag
      421 gtcagagaac ggccgatcga accgggcaac ttggcttcat cgtcacctga tctcaccctg
      481 gcgaagtgct cggtgagacc ctcttcggca tcgacgcggg cgccgtcgag gacgacaccg
      541 gcggtgatct tcgccagctc gtggggctcg tgatcaccct gtgccacctt tggcgacggc
      601 cggaagcctt tggcgttgct ccggtagacc ttctgcaaca tggccccggc tttccgggtc
      661 tcgacaaccg agatgatccc ggcctcctcc agcaggttga agtggtagta gagccgcgtg
      721 gtgggcacgt cgagcagctc cgccacctct cttatcgacc tcggttcctc gagatacctg
      781 atgatgcgaa aacgagtggt gttgttgagg acctcgaaga cttcgatgtc ttcgatctcc
      841 atgaactccg cttgttccga catcagacct ccgtgaccgg tttcgagacg ctggtgcctc
      901 gttgcagacg ctctcccatg tccacgagca ctcgtcccaa catcggtgcg aaggaatgac
      961 gctcctgacg ccgacggggt tggatctgac gctgccgttc ctcttggatg tatctcgctg
     1021 tgtgcaccgt ttcgcggtac atgactctct cctcggcccc ctcgatcact cgtcttcggg
     1081 gtgccgccgc gatcatactt tcaactgatc tgaatattca agcgatttga aacgtatgac
     1141 gttcaattga aagtgaccac ggatgaatgc ttaacctcgc catgtgcggt tagggatcga
     1201 tctcgacggc gtcgtcgcca acttcaccaa aggctggatg agcttctaca acgagcagtt
     1261 cggcacggcc ctggcagtgg aggattcaaa acgctggcac gatctcgtcg agctgaccca
     1321 cttcgatcac atcggtgagt tttgggagtg gtccgccgac ctcgacgggc gctcggtgtt
     1381 ctggcacctc gagcccttcc ccggagctgt cgaggcgctg cgctccctgg cggacgccgg
     1441 ccaccacgtg atcgtcgtca ccaccaagcc ggatttcgcg gttggcgaca catacgaatg
     1501 ggtagaacga catcgaatcc ccgctgcgga gatccacatc ctcgaggaca agtggctcgt
     1561 cgaatgcgac gtctatctcg acgatggccc tcacatcctt cccgggctgg tcgagcaccg
     1621 accggactcg acggtgtgcc gttacgtccg accatggaac gacccggtcc ccggggccat
     1681 cgacgtcaac gacttcgtcg agtttcgcga ggtcgtcgat caactcgccg gaggcgaggc
     1741 cgcgccacta gcctcgccgc acgatctctg atcatccctg ggagcgggcg aaagcaccag
     1801 gggtggacag atcacccatg gggaaacaca aggagcggaa aacatgaacc gaagagccct
     1861 ttgggctttg atcgcggtgc ttgccctcgt agtagcggca tgcggtgacg gcgagggtgg
     1921 cgatgacacc accaccaccg ccgcacccgg tacgacggca cccgatgaga ccactaccac
     1981 agaagacatg acgacaacca ccgggggcga gatgtccgat tggccagaca cgctggtctt
     2041 cgggttcgtg cccagccagg aggcgaccga gctccaagac gacgtcgaca cgttcgccgc
     2101 gatcctgagc gacgccctgg ggatcacggt cgaaggcacg gtgaccaccg actacacagc
     2161 cctcgctctg gcgatgggga cgggacaggc agacatgggc gccttcggtc ccgccggtta
     2221 cgtcatcgcc tcacgggagt tcgacaacct cgagctgttg gcgcagtcgg tgcgcttcgg
     2281 cgactccacc taccacgccc agtacttcac caacgatccg tcgatctgtg gcgatgccgg
     2341 gaccgaggag ggcgccttct actacgacga ctccggagcc gtggttcc
//
LOCUS       DASUTA010000289         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_860123, whole genome shotgun sequence.
ACCESSION   DASUTA010000289 DASUTA010000000
VERSION     DASUTA010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860123"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VFZ80_05730"
     CDS             complement(<1..625)
                     /locus_tag="VFZ80_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6286966.1"
                     /translation="MISTIGDRAEEAARGSGNLALAISDGTQLSWSEMFDEFAPALTS
                     YARSRGVREAEDLVQDVFVAAFKQLPGFVGDRSGLRSLLFTIAYRRIADHHRQRYRRR
                     ETLVADHPPRADPGPTVEQIVTLGDTAGRAVQALALLSDRERRVMEMRIFEEDSPANV
                     GRALGLSSGNVRVIQARALAKIRKHLRSTGDNGFALPIVTYGALSDSF"
     gene            801..1091
                     /locus_tag="VFZ80_05735"
     CDS             801..1091
                     /locus_tag="VFZ80_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286967.1"
                     /translation="MTTIKTVCVRCGGIRLTPDDVALELRPGRREGAYRFKCPTCAVP
                     HRKPANPRVVRVLLATGVAFDVVNPDPITEVEITAFAVALEAETDPLRLLTG"
     gene            1116..>2303
                     /locus_tag="VFZ80_05740"
     CDS             1116..>2303
                     /locus_tag="VFZ80_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX6286968.1"
                     /translation="MGGRPGSIAPYLRIGGLIALSLAVALLLAASIGGDEIADDTTVL
                     RRAESVLGTTTLVDLTARETLANARTWSHGETTSDQLDRANAASADMVGRLRDQTSRL
                     EEAVAAKHGLGGSSIAQSMADLDRAAEEMATAIDARRIDEAGQVAETRLEPAIAALMT
                     KVTSLRDDREAHIASVSEGLGLVVDAGRFLVALTLAVLVWTLIVGAKRLGQKDTLPTS
                     LDTESELHRKREVFLAAASHHINTPLTGVVGLAELLEDRTRDLNGGVRSEIIDLLAIQ
                     ARETAHVVDDILVAARSDLSELIVFEDKVDVRRVVEQAISGIGSTLRSRVTIRGHGIA
                     DADRKWVEHIVRNLIRNAGSYGGENIGVRIHTVHSKVVVDVADDGDGLPTGAEDVVFR
                     TPYY"
BASE COUNT          450 a          724 c          755 g          374 t
ORIGIN      
        1 ggaaggagtc agacagggcg ccatacgtga cgatgggcag ggcaaagcca ttgtcgcccg
       61 tcgagcgcaa gtgcttgcga atcttcgcca acgcacgcgc ctggatcacc cggacattcc
      121 cgctgctgag acccagcgca cggccgacgt tggccggtga gtcctcctcg aagatgcgca
      181 tctccatgac ccgtcgctcc cggtcgctga gcagggctaa ggcctgtacg gcccggccgg
      241 ccgtatcacc cagggtcacg atctgttcga cggtcgggcc cggatcggcc cttggcgggt
      301 gatcggcgac cagggtctcg cgacgccgat agcgctgacg gtggtgatcg gcgatgcgcc
      361 ggtaggcgat ggtgaacaga agtgagcgca gacccgacct atcgcccacg aaccccggca
      421 gctgcttgaa cgcggccacg aacacatcct ggacaaggtc ctcggcctcc cgcactccac
      481 gggagcgggc ataggaggtg agcgccgggg cgaactcgtc gaacatctcg ctccacgaca
      541 gctgggtgcc atcggagatg gccaacgcca ggttgccgga acctctcgca gcttcctcag
      601 ctcgatcgcc gatggtggat atcaccaagt catgtctcgg gttggggttt cccaagacac
      661 tacccccaca cccactggaa gcctatgggg aaaagaccgt gtaacgccac cggagcagcg
      721 gcgatagcaa gggaagggcg gacagacgat ggcgaggagg acccgaaaga ctggccagca
      781 accaacgccg gagaacgagg atgacgacca tcaagacagt ctgcgtccga tgcggaggca
      841 tcagattgac gccggacgac gtggccttgg agctgcgccc cggccgccgc gagggtgcgt
      901 accggttcaa gtgcccgact tgcgccgtcc cgcaccgcaa acctgccaac cctcgggtgg
      961 tacgcgtgct gctggccacg ggggtggcat tcgatgtggt caatccggac cccatcacgg
     1021 aggtcgagat cacagccttt gcggtcgccc tcgaggccga aacggaccca ctgcggctcc
     1081 tgaccggctg agccggtcaa tgagaggagg gactgatggg tgggcgaccc ggctccattg
     1141 ctccatacct tcggatcggt ggcctgatcg cgctgtcgct ggctgttgcg ctactgctag
     1201 cggccagcat tggtggagac gagatcgcag acgacaccac ggtcctgcga cgggcagagt
     1261 cggtcttggg cacgaccacc ctggtcgacc tgacggctcg agagacgttg gcgaacgctc
     1321 gcacctggag ccacggtgag acaacgtcgg accagctgga tcgggccaac gccgccagcg
     1381 ccgacatggt cggacgactt cgcgaccaga cgtcacgtct cgaggaggct gttgccgcca
     1441 aacacggcct cggcggctcc tccatcgccc aatcgatggc cgacctggat cgggccgcgg
     1501 aggagatggc gactgcaatc gacgcgcgtc gcattgacga ggccggccag gttgcagaga
     1561 ccaggctgga accagcaata gccgcgctga tgacgaaggt gacctcattg cgtgacgatc
     1621 gggaagctca catcgcatct gtgtccgagg gtctcggcct cgtggtcgat gcaggtcgat
     1681 tcttggtcgc actgaccctg gcggtactgg tgtggaccct catcgtgggc gcgaaacggc
     1741 tcgggcagaa agacaccctg cccacaagtc tcgatacgga gagcgagctc catcgcaaga
     1801 gagaggtctt cctcgccgcc gcgtcccatc acatcaacac accgctgacc ggtgtggtcg
     1861 gcttggccga gctgctcgag gacaggacca gggatctgaa tggcggtgtc cgcagcgaga
     1921 tcatcgatct attggcgatt caggcacggg agaccgctca tgtcgtcgac gacatcctcg
     1981 tcgctgctcg gtcggatctc agcgagttga tcgtgttcga agacaaggtg gatgttcgtc
     2041 gtgtcgtcga gcaggcaatc tccgggatcg gcagcacgct tcgctcccgc gtaaccatcc
     2101 gcggccatgg aatcgctgat gccgatcgca aatgggtgga gcacatcgtc cgcaacctga
     2161 tcagaaacgc cggttcctac ggaggtgaga acatcggcgt gaggatccac acggtgcaca
     2221 gcaaggtcgt agtcgacgta gccgacgacg gcgacggtct gcccaccggg gccgaggacg
     2281 tcgtctttcg gactccctac tac
//
LOCUS       DASUTA010000290         2775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_990695, whole genome shotgun sequence.
ACCESSION   DASUTA010000290 DASUTA010000000
VERSION     DASUTA010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990695"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VFZ80_05745"
     CDS             <1..927
                     /locus_tag="VFZ80_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX6286969.1"
                     /translation="LYEMAKERDVPGRSGMDKDELVEALADGRDEEGPGARVQSPGRA
                     ETTERSIWRGAITFGLITIPVGLYSATQDRDISFRLLSSEDGSRIKYKRVSSETGEEV
                     AWDDIVKGYEYEKGHFVTFTHEEMDRMPVDSAHTIDVVQFVNETEIDPIGFEKSYYVA
                     PEPTASKAYELLVRALVRSGRVAVAKITLREKESLCELRPVDGVLVLQTMHWPDEIRI
                     PSFSQLEERAEVSEAELKMAEQLIDQLSGSFDPSSFEDSYRMALEKAIQDKIEGNEIE
                     LSPEEPEPAKVTDLLEALKASVEATKQEKKSA"
     gene            complement(919..1398)
                     /locus_tag="VFZ80_05750"
     CDS             complement(919..1398)
                     /locus_tag="VFZ80_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX6286970.1"
                     /translation="MLLGEQPGDREDHDGEPFVGPAGELLDAALTQAGSERKEVYITN
                     VVKHFRFEERGQRRLHKKPLRRHVNACRSWLNAESAHVRPDVIVALGGSAAQSVLGRS
                     IRVMSDHGVPIEWEGVLVLSTIHPSFVLRSSGDSERRDELFDLLVSDLKTSRELASG"
     gene            complement(1534..2550)
                     /locus_tag="VFZ80_05755"
     CDS             complement(1534..2550)
                     /locus_tag="VFZ80_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEX6286971.1"
                     /translation="MTTETADLAIEAGLRYVEAGEPGFRRVRRGRGFSYENQRGVAIN
                     GSKRKWIESLVIPPAWDQVWICADTQGHILATGVDKAGRKQYIYHPEWETIRDAVKFD
                     RLGAFGRGLSALRKQIDSDLRTRGLPRARVVALATAVLDRTLLRVGNGKYARENESYG
                     LTTLTTDHIQVNGNHVHLEFAGKGGADRQLAFRDRRLSSLVARCQELAGQTLFSYETE
                     AGDVGSVTSSDINRYLSASMGARYTAKDFRTWGASALVVTELARAGEGADREDALRSA
                     VDRAAERLGNTRAVCRNSYLHPAVITATETGSLSAAWKRSRPGKWIDRSESALRLVLE
                     AEGG"
BASE COUNT          536 a          878 c          872 g          489 t
ORIGIN      
        1 ctctacgaga tggccaaaga gcgagatgtc cccggccgtt ccggaatgga caaggacgag
       61 ctcgtcgaag cactggccga tggacgagac gaggagggcc ccggcgcccg ggtgcagagc
      121 ccgggtcgag cagagaccac cgagcgttcc atttggagag gcgcgatcac atttgggctg
      181 atcaccatcc cggtcgggct gtacagcgcc acgcaggacc gtgacatctc cttccggctg
      241 ctgtccagcg aggacggttc gcgaatcaag tacaagcggg tgtcctccga gaccggcgag
      301 gaggtcgcat gggacgacat cgtcaagggc tatgagtacg agaagggtca cttcgtcact
      361 ttcacccatg aagagatgga ccggatgcct gttgacagtg cgcacacgat cgacgtggtg
      421 cagttcgtga acgagacgga gatcgacccg atcgggttcg agaagtccta ctacgtagcg
      481 cccgagccga cagcctccaa ggcctacgag ctcctggtgc gggccctggt tcgatcgggc
      541 cgggtagccg tggccaagat cacattgcgt gagaaggagt cgctgtgcga acttcggcct
      601 gtcgacgggg tcctggtgct ccagacgatg cactggccag acgagatacg aatcccgagt
      661 ttcagccaac tcgaggaacg cgccgaggtt tccgaagccg aattgaagat ggcagaacag
      721 ctgatcgacc agctcagcgg ttccttcgac ccatcctcat tcgaggactc ctatcgcatg
      781 gccctggaga aggccattca ggacaagatc gagggaaacg aaatcgagct ctcgcccgaa
      841 gagccggaac cggcgaaggt gaccgacctt ctcgaagcgc tcaaggccag cgtcgaagcg
      901 acaaaacagg aaaagaagtc agcctgacgc caactctcgt gaagtcttca gatcggagac
      961 gagcaggtcg aacaactcgt ccctgcgttc actgtcaccg gaggacctga ggacgaacga
     1021 ggggtgaatg gtcgacagca ccagcacccc ttcccactcg atggggactc cgtgatcgct
     1081 catcacccgg atcgatctcc ccagaacgga ttgcgcggcc gagccaccga gggcgacgat
     1141 cacatcaggt cgaacatgcg ccgactcggc gttgagccag gaccggcatg cgttcacatg
     1201 acggcgcaac ggcttcttgt gtaatcgccg ctgcccacgc tcctcgaacc tgaagtgctt
     1261 gacgacattg gtgatgtaca cctccttccg ctcgctgccc gcctgggtca gcgccgcatc
     1321 cagcagctca cccgccggcc cgacgaaagg ctccccatcg tgatcttccc ggtcaccggg
     1381 ctgctctccg aggagcatga tcgaggcacc aacgggtccc taaccgaaca tgacctgagt
     1441 cgccttctcg tggagctcac aggcggcagc cttcggcgag atcggccgct tgctccaagc
     1501 tcgtactctc gagatcgagc tcggcaggat cggtcatccg ccctcggcct ccaggaccag
     1561 tcgtagtgcc gactccgagc gatcgatcca cttcccgggc cgggacctct tccatgcggc
     1621 cgagagactt ccggtctcgg tggcggtgat gaccgctggg tgcaggtatg agttgcggca
     1681 cacggcccgg gtgttaccga gacgctcggc ggcccggtcg acggccgagc gcagggcgtc
     1741 ctcacggtcc gctccttcac cggcgcgagc cagctctgtg accaccaggg cgctcgcacc
     1801 ccaggtccgg aagtccttgg cggtatagcg ggcgcccatt gacgcggaga gatagcgatt
     1861 gatgtccgag gaggtgacgg agcccacgtc gccggcctcg gtttcgtaac tgaacagggt
     1921 ctgtcctgcc agctcctggc accgggcgac gagcgaggag agacgacggt cacggaaggc
     1981 gagttggcga tcggcgcccc ctttcccggc gaactcgagg tggacatggt tcccgttgac
     2041 ctggatgtgg tcggtggtga gtgtggtgag cccgtatgac tcgttctccc gggcgtattt
     2101 tccgttcccg acccgtagaa gtgtcctgtc gagaaccgcc gtggccagag cgacgacgcg
     2161 ggcccgaggc agacctctcg tacgcagatc cgagtcgatc tgcttccgca gagcagagag
     2221 cccgcgccca aaggctccga gccggtcgaa cttgaccgcg tctcggatgg tctcccactc
     2281 ggggtgatat atgtactgct tgcgcccggc tttatccacg cctgtcgcca ggatgtgccc
     2341 ttgggtgtcg gcgcagatcc aaacctgatc ccacgcaggc gggatcacca gactctcgat
     2401 ccacttgcgc ttgctcccgt tgatcgcgac tcccctttgg ttctcgtagg agaatccacg
     2461 tccgcgacga acacggcgaa agccgggctc gcctgcctcc acatagcgga gcccagcctc
     2521 gatggcgaga tcggcggtct cggtcgtcat atgggcacag ggttcccaag cacgaatgcc
     2581 tccaaaccac tccgtccggg ttttcaccaa gtcccacagg ggtaatcgcg gtcaatgagc
     2641 ctgaccgggc cgacgacacg tcccagcggt gacacgactc cgcgtcggtg ggagacagtt
     2701 ctgaggacac tgaccattgc gctggtgatc ggtctcgtcc tggcgactgg ggtagggctt
     2761 ctcggtgtgc gctct
//
LOCUS       DASUTA010000291         3878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1250560, whole genome shotgun sequence.
ACCESSION   DASUTA010000291 DASUTA010000000
VERSION     DASUTA010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250560"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VFZ80_05760"
     CDS             <1..388
                     /locus_tag="VFZ80_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003987603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX6286972.1"
                     /translation="EAEGLVRAAGELFRVAAELSVPTGTIVTRRGFGLGAQAMAAGGF
                     HEPRFTVAWPTSEFGPMGLEGAVKLGFRRELEAIEDPDERQTELERRVEEMAEAGSGI
                     NVAMAFEIDDVIDPADSSRWITTLLF"
     gene            complement(445..1413)
                     /locus_tag="VFZ80_05765"
     CDS             complement(445..1413)
                     /locus_tag="VFZ80_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEX6286973.1"
                     /translation="MNRQLFPQNVIAMIWDFDKTLIPGNMPGPLFRHFDIDQSVFWAE
                     VDGLEQFYRSHGSTLIQREHLYLNHILTYVREGRMPGLGNGLLRELGAEIEFYTGMPD
                     FLELARKTIESQPRFAEHQIRLEHYVVSTGLRQMILGSSIAPQLDGVWACEFTELVAP
                     PGYGEVQDRLFSPEGEIQQLIYTIDNTSKTRAIFEINKGTNKNANIDVNAKIAREDRR
                     VPFQNMIYVADGPSDVPVFSVVEANGGRTYAVYRPGSQSEFDQAARLLEQDRVDAFGE
                     ADYSDGSHTAMWLMHAAETIANRIVSDREEALNERMGLPPRHVGED"
     gene            complement(1458..1865)
                     /locus_tag="VFZ80_05770"
     CDS             complement(1458..1865)
                     /locus_tag="VFZ80_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEX6286974.1"
                     /translation="MESTEIQIHTGGEKAAADITAQAAEFCRGKGDGLLSIFVPHATA
                     GVAIFETGAGSEADVLSALDDLIPAETGRWRHEHGSPGHGRDHVLPAFISPSTSIPVI
                     EGRLALGTWQSIVLVDTNVDNPSRRVRFSFLPG"
     gene            1900..2316
                     /locus_tag="VFZ80_05775"
     CDS             1900..2316
                     /locus_tag="VFZ80_05775"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX6286975.1"
                     /translation="MPFDLTPEQVDVFVAEVFPASYADGMRCIAIAEGYAEVRWPFDE
                     KKLRPGAYIPGPVMFALADSALWYATFSVIGLEPMAVTSEMSIRYLRPAQGADLLGRA
                     TINSVSTRRIVGTIEMWVDGKPEKLVAVAQGSYARP"
     gene            complement(2329..3204)
                     /locus_tag="VFZ80_05780"
     CDS             complement(2329..3204)
                     /locus_tag="VFZ80_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286976.1"
                     /translation="MTDLDRTVLRTSINQLYAAELEALGERTYQFLDEARKWDFGSTL
                     ANGGVVSFAHVNVGDCGLHVAAAVNAALDSGADTLLGIGVLHAFTAEMELARRDVSSG
                     GGSPSSHSLWGIQGPGLDYRDEWTGDHSMRALRHFWAAETERRGLADRRLVERYAFLA
                     GGHPEELPNVEETAALAENAVIVATGDQFHHGIAYGTPEQDALPMEPDGLEAARASMQ
                     TGIDLIAAQDHGGYDRHCVTAKSDDRDTVQLFSFLRGPLQGELIEIGASDATELYDAP
                     APSWAAGGFIKFERL"
     gene            complement(3230..>3878)
                     /locus_tag="VFZ80_05785"
     CDS             complement(3230..>3878)
                     /locus_tag="VFZ80_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286977.1"
                     /translation="TANSLDSTSDSIVSASVTLDGALTTLEGVADFLEGELPETLESV
                     QTSMPAAIQAANAVDGTLRALSLVGVEYDPDEPFGESLSRVNTALSTLPNEIRTQSES
                     LRLLIPSAEQLAGDTAGLASSLEEVEESLAGFTSLTATYQATIDEAETTIAATSETID
                     LNIWMIRALILTTGLLGVLVGMVLWSLAQRLDTLTGTVDLLHHERQEVVVVTPSD"
BASE COUNT          652 a         1159 c         1320 g          747 t
ORIGIN      
        1 cgaggcggaa gggttggtgc gtgccgccgg cgagctgttt cgggtggctg ccgagttgtc
       61 ggtgccgacc gggaccatcg tcacccgtcg tggtttcggg ctcggggccc aggccatggc
      121 cgccggtggc ttccacgagc cccgcttcac cgtggcttgg ccgacgtcgg agttcgggcc
      181 gatgggtctc gaaggcgcgg tgaagctcgg gtttcgaaga gagctcgaag cgatcgagga
      241 tcccgacgag cgtcagaccg agctggagcg ccgggtcgag gagatggcag aggcaggtag
      301 cggcatcaac gtggccatgg ccttcgagat cgacgacgtg atcgaccccg ccgactcctc
      361 acgctggatc accactctgc tgttctagaa ccgttctgtg aacgcagcgc gacacatagt
      421 gtcgcgtctc gttcacagaa cggttcagtc ctcgccgacg tggcgtggcg gcagccccat
      481 gcgctcgttg agggcttctt cgcggtcgga gacgatccgg ttggcgatgg tctcggctgc
      541 gtgcatgagc cacatggcgg tgtgtgagcc gtcggagtag tcggcttcac cgaacgcgtc
      601 gacgcggtcc tgttcgagaa gacgtgccgc ctggtcgaac tcgctctgag atccggggcg
      661 atagacggcg taggtccgac ccccattggc ctccaccacc gagaacaccg ggacgtctga
      721 cgggccatcg gcgacgtaaa tcatgttctg gaacggcacc cttcggtctt cccgggcgat
      781 cttggcgttg acgtcgatgt tggcgttctt gttggtcccc ttgttgatct cgaagatggc
      841 acgtgtcttc gacgtgttgt cgatggtgta gatgagctgt tggatctcgc cctcggggga
      901 gaagagacga tcctggacct cgccgtatcc cggcggtgcg accaactccg tgaattcgca
      961 tgcccacact ccgtcgagct ggggtgcgat ggacgatccg aggatcatct gccgcagccc
     1021 ggtggacacc acgtagtgct cgagacggat ctggtgctcg gcgaatcggg gctgggactc
     1081 gatggtcttc cgggccagct cgaggaagtc cggcatcccg gtgtagaact cgatctcggc
     1141 cccgagctcg cggaggagac cgttgccgag accgggcatc cggccctccc tcacgtaggt
     1201 gaggatgtga ttcaggtaca ggtgctcccg ctggatcagt gtggagccgt gcgacctgta
     1261 gaactgctcg agcccgtcga cctctgccca gaagacggac tggtcgatgt cgaaatggcg
     1321 aaacaggggc cccggcatgt ttccggggat caaggtcttg tcgaagtccc agatcatggc
     1381 aatgacgttc tgggggaaga gttggcggtt catgccggta cgaaggctat tcggtaatcc
     1441 acaggcttca acctcggcta cccgggcagg aagctgaacc tcaccctgcg tgacgggttg
     1501 tcgacgttgg tgtcgaccag cacgatcgac tgccaggtgc ccagggcgag tcggccctcg
     1561 atgaccggga tcgatgtcga cgggctgatg aacgcgggca agacgtgatc gcgaccgtgc
     1621 ccgggactgc cgtgctcatg acgccaccgc ccggtctcgg cggggatcag gtcgtcgagc
     1681 gcagagagca cgtcggcctc gctgccggcg cctgtttcga agatggcgac accggcggtg
     1741 gcgtggggga cgaagatcga gaggagcccg tctcctttgc cgcggcagaa ctcggctgcc
     1801 tgggcggtga tgtcggcggc ggccttctcg ccgccggtgt ggatctgaat ctcggtcgat
     1861 tccatgaaac cagtttcaca gaacgagggt ctattgacag tgccgttcga tttgacccct
     1921 gagcaggtgg atgtgttcgt ggccgaggtc ttccccgcct cctacgcaga cggcatgcga
     1981 tgcatcgcca tcgcggaggg atacgccgag gtgagatggc ccttcgatga gaagaagctg
     2041 aggccgggcg cctacatccc ggggccggtg atgttcgcgt tggccgacag cgctctctgg
     2101 tatgccacct tctcggtgat cggtctcgag ccgatggcgg tgaccagtga gatgtcgatc
     2161 aggtacctgc gaccggctca gggagcggat ctgctcgggc gggcgacgat aaattcggtg
     2221 agtacccggc gcatcgtcgg caccatcgag atgtgggtgg atggcaagcc ggagaagctg
     2281 gtggcggtgg cccaggggag ttacgcacgg ccctgaccca gtgcggattc agagcctctc
     2341 gaacttgatg aaaccgccgg ccgcccacga cggcgccggc gcgtcgtaga gctcggtggc
     2401 gtcggatgct ccgatctcga tgagctcgcc ctgcaggggc ccgcgcagga agctgaacaa
     2461 ctgaactgtg tcgcgatcgt cgctcttggc cgtgacgcag tgccggtcgt atccgccgtg
     2521 gtcctgagcc gcgatgagat cgatccccgt ctgcatgctg gcacgcgccg cctccaggcc
     2581 gtcgggctcc atgggcagag cgtcttgttc cggggtcccg taggcgattc cgtggtgaaa
     2641 ctgatccccg gtggcgacta tgaccgcgtt ctcggcgagg gcagccgtct cctcgacgtt
     2701 gggaagctcc tcaggatgtc cccctgcgag gaatgcgtac cgctccacca accggcggtc
     2761 ggcgaggcca cgtcgctctg tctctgccgc ccagaaatgt cgcagcgctc tcatggagtg
     2821 gtccccggtc cactcgtcgc gatagtcgag gccgggccct tggatccccc agagggaatg
     2881 gctcgacggg gatcctccac ccgatgacac gtcacggcga gccagctcca tctccgcggt
     2941 gaaggcgtgg aggactccga tgccgagaag ggtgtcggcc ccactgtcga gcgccgcgtt
     3001 gacagcggct gcgacatgca aaccgcagtc gcccacgttg acgtgggcga agctgaccac
     3061 gccaccgttt gccagtgtcg agccgaagtc ccacttcctg gcctcgtcga ggaattggta
     3121 ggtccgctcg cccagggctt ccagctctgc cgcatacagc tggttgatcg aggtgcgcag
     3181 aacggtccgg tcgaggtcgg tcatgaccgg aaccttacgg cttcttcgat cagtcgctcg
     3241 gcgtcaccac gacgacctcc tggcgttcat gatgtagaag gtccaccgtt ccggtgagtg
     3301 tgtccagacg ttgcgccaaa gaccacagga ccatccccac cagcacgccg agcaatcccg
     3361 tggtgaggat gagcgctctg atcatccata tgttgaggtc gatcgtctca ctcgtggcgg
     3421 cgatggtggt ctctgcttca tcgatggtcg cctggtatgt cgctgtcaga gaggtgaagc
     3481 ccgccaggct ctcttcgacc tcctccaagg aggaagcaag gcctgcagtg tccccggcga
     3541 gctgttctgc ggaggggatg agcaggcgca atgactcgct ttgggttctt atctcgttgg
     3601 gaagcgtcga caacgcggtg ttgacccggg acagagactc gccgaagggc tcgtcggggt
     3661 catattcgac accgaccaaa gagagggctc gcagggtgcc gtctacggcg ttggcagcct
     3721 gtatcgccgc cggcatagag gtctgcaccg attccagcgt ctcaggtagt tcgccttcga
     3781 ggaagtcggc aacgccttcg agtgtggtca aggctccgtc gagagtgact gatgccgaga
     3841 cgatgctgtc cgaggtggag tcgaggctgt tcgcggta
//
LOCUS       DASUTA010000292         3105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_732997, whole genome shotgun sequence.
ACCESSION   DASUTA010000292 DASUTA010000000
VERSION     DASUTA010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732997"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1352
                     /locus_tag="VFZ80_05790"
     CDS             162..1352
                     /locus_tag="VFZ80_05790"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEX6286978.1"
                     /translation="MPSDRSDFETLIGPDRRRVPRPRGLAAIVLAVGGAILVGLVALW
                     PTDTPVLDLSEVGFADEVVRAETVTATVGPCSYDATLDCRLYQLTLIEAPVGDIALLE
                     FPDEPGQPDLMEGDIVYLAVVEFDDGTVDYQYAGLDRRVLLVVLALVFAVAVIGLGRL
                     RGVAALIGLALSIGILLWFILPAIISGEDAVMVALVGGGAIAIVSLYLAHGYNPLTHV
                     AALGAFSALALTTALSWLTVEVANFSGLVTEESFFLLAIPDLDLNGLLLAGIVLGAIG
                     ALDDVTVTQASAVWEVHGANPTMGFDELFESGLRVGRDHIVSTVNTLLLAYAGASLPL
                     LLFFTLSNQSLGFIASSEVVAIEIVRTLVGSIGLVAAVPVTTWLSARVVSTLADQSST
                     RVSS"
     gene            complement(1413..1598)
                     /locus_tag="VFZ80_05795"
     CDS             complement(1413..1598)
                     /locus_tag="VFZ80_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286979.1"
                     /translation="MLRDFTSTSRYMHAEYVRRRVNHPEPLEQPRHGLRKALGQSLIH
                     LGERLARIDPQPVDEAA"
     gene            complement(1591..2730)
                     /locus_tag="VFZ80_05800"
     CDS             complement(1591..2730)
                     /locus_tag="VFZ80_05800"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6286980.1"
                     /translation="MSESTSPLERVRTLTTAPRRIPIEVEGNVSHEVILTIWAVFSPK
                     DTHSAADLGPEWIEEVRAKTPDDLAAELISLGGPWFYVWLSISAFLLSAPHPHDPDHV
                     FNWLESIDEQRLRRWILGHTCHSTDQALIEQAATGDIDAVVELVSPNGDEEESAEANE
                     MVEYARQVLFSDGLPERFARVIKRFREEVFTEYEDEFAGAISRAAAARRAAPARGSAK
                     EVVEEVTSGIDFEIPIGITRVVLVPSVITRPLSLVDSARGTLVVYYGIADEFVDSDPE
                     APPSWLVRMYKALSDDKRLRILRRLSEGETSLDELTEMLGLSKSTVHHHLSQLRGAGL
                     VRVRIPSDESKSKQKHYTLREQSLSDAGAFLDSYLRTGDLEEQHA"
BASE COUNT          511 a          982 c          965 g          647 t
ORIGIN      
        1 cacgagcggc accgatcgac gacccggacg tgaggagagg catcttcaca cactcttcca
       61 cgtcgtggta cacgacccag gcggagttgg atcgcctggt cgccacatct ccgatggtgg
      121 agatcatctt cgaggactga catcgggaat accatcgtct ggtgccaagc gaccggtccg
      181 acttcgagac cctgatcggg cccgaccgcc gccgggttcc tcggccccgc gggctggccg
      241 ccatcgtgct cgcagttgga ggtgcgatcc tggtggggtt ggtcgccctc tggccgaccg
      301 acacgccggt gctcgacctg tccgaggtcg ggttcgccga cgaagtggtc cgggcggaga
      361 ccgtgaccgc caccgtaggc ccgtgttcct acgatgccac gctcgactgt cgtctgtacc
      421 agctgactct catcgaagcg cctgtcggcg atatcgcact tctcgagttt cccgacgagc
      481 ccggacagcc cgatctcatg gaaggcgaca tcgtctacct cgcggtggtc gagttcgacg
      541 atgggacggt cgactatcag tacgccggcc tcgaccgccg ggtgcttctg gtggtcttgg
      601 cgctggtctt cgccgttgcc gtgatcggtc tcggtcggct caggggggtt gccgctctca
      661 tcggtctcgc actgagcatc ggcatcctcc tctggttcat cctccccgcc atcatctccg
      721 gcgaagacgc cgtgatggtc gccttggtag ggggaggggc tatcgccatc gtcagcctct
      781 atctggctca cggctacaac ccgctcactc atgtcgccgc tctcggcgca ttctccgcac
      841 tcgccctcac cacggccctc tcctggctca cggtcgaggt cgcaaacttc tccgggctcg
      901 tcaccgagga gtcgttcttc ctgctggcga ttcccgacct cgatctgaac ggactactcc
      961 tcgccgggat cgtcttgggg gccatcggtg ccctggacga cgtgacggtg actcaggcct
     1021 ccgcggtgtg ggaggtgcat ggcgccaatc cgacgatggg cttcgatgag ctgttcgagt
     1081 cgggtctccg tgtggggcgt gatcacatcg tctccaccgt caacaccctg cttctcgcct
     1141 atgccggcgc ctcactgcct cttctgctct tcttcacatt gtccaaccag tcgctcggct
     1201 tcatcgccag ctcggaagtg gtggcgatcg agatcgtccg aacactcgtc ggttccatcg
     1261 gtctcgttgc tgccgttccg gtgacgacgt ggctcagcgc tcgggtggtc tcgacgttgg
     1321 ctgatcagtc gtcgacccgg gtctcctcct aggagaaagg agacccaggg cgacggagtc
     1381 cgaaaccgcc tccccagttc ggaggttctg ggtcaagcag cctcgtcgac gggttgggga
     1441 tcgatcctgg cgagacgctc acccaggtgg atgagactct ggcccagggc ctttctcaac
     1501 ccgtgacggg gctgttcgag tggctccgga tgattgaccc ggcgacggac gtattcggcg
     1561 tgcatgtatc tcgaagtgga cgtgaaatcc ctaagcatgt tgttcctcca ggtcgcctgt
     1621 cctcaagtag gagtcgagga aggctccggc gtctgaaagc gattgttcgc gcagcgtgta
     1681 gtgcttctgt ttcgacttcg actcatcgct cgggatgcgg actctgacca gtccggcgcc
     1741 cctcagctga ctgagatggt gatgaacggt ggacttcgac aagccgagca tctcggtgag
     1801 ctcgtcaagc gacgtctcac cttcactgag acgacggagg atgcgaagac gcttgtcgtc
     1861 gctcaatgcc ttgtacatgc gcaccagcca cgagggcggg gcttcggggt cgctgtcgac
     1921 gaactcatcc gcgattccgt aatagacgac gagtgtcccc cgggcgctgt cgacgaggga
     1981 gagcggccgg gtgatgacag agggcacgag aaccactcga gtgattccga tcgggatctc
     2041 gaagtcgatg ccactggtga cctcctcgac cacctccttg gcgctgcctc tcgccggtgc
     2101 ggcccgccgt gctgcggcgg cccggctgat tgcgccggcg aactcgtcct cgtattcggt
     2161 gaagacctct tcccggaatc tcttgatgac tcgggcaaaa cgctccggca ggccgtcgct
     2221 gaacaggacc tggcgggcgt actccaccat ctcgttcgcc tcggccgact cctcttcgtc
     2281 cccattgggt gacaccagct ccacaacggc gtcgatgtcg cccgtggccg cctgctcgat
     2341 gagcgcctgg tcggtgctgt ggcaagtgtg accgaggatc cagcggcgga gccgctgctc
     2401 atcgatcgac tccagccagt tgaatacgtg gtcggggtcg tggggatgag gcgccgacag
     2461 aagaaaggcg gaaatggaga gccagacgta gaaccagggc ccaccaagac tgatcagctc
     2521 ggcggcgaga tcatccggag tcttcgcccg aacctcttcg atccactccg ggcccagatc
     2581 ggcggcggag tgggtgtctt tcgggctgaa caccgcccaa atcgtcaaga tcacctcgtg
     2641 ggacacattg ccctcgactt cgatcgggat acgacgcgga gcggtggtca gagttcgaac
     2701 tcgttctaat ggtgatgtgg attcagacat ttctgatgcc agcgggtgat tcgatcgata
     2761 tcgaatggga gtattgcaca tcgacatcga tctgtccaac gaaattcgat gaacatcgaa
     2821 ttcagcttcg atgaaccagg agtttcgatc ccacatgtgg tttctgcagg ggtggtgccc
     2881 tggcccgggg actgaccaac ctgagatctg tcgtccgagt acccctacac tcgccgccca
     2941 tggtcgaggt tcgtcgtccc actgcggcac aggtgttgga gaccctgggc gcgctgagcg
     3001 cggtggggac cttggccctg ggcttcctcg gcaccctgcc cgagcatcca catttcgaga
     3061 tcggtcgtga ggtcttcggc aacatccccg atgcggtcgt cctcg
//
LOCUS       DASUTA010000293         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1510459, whole genome shotgun sequence.
ACCESSION   DASUTA010000293 DASUTA010000000
VERSION     DASUTA010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510459"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..421)
                     /locus_tag="VFZ80_05805"
     CDS             complement(68..421)
                     /locus_tag="VFZ80_05805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX6286981.1"
                     /translation="MIADHEELARHRTIDLTTIGRKSGMPRTIEIWWFQVGGRFVITG
                     TPGKRDWLANVLANPNVTIHVAGRDIAARAAPIDDPDVRRGIFTHSSTSWYTTQAELD
                     HLVATSPMVEITFED"
     gene            complement(456..1340)
                     /locus_tag="VFZ80_05810"
     CDS             complement(456..1340)
                     /locus_tag="VFZ80_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX6286982.1"
                     /translation="MANSAPIEKLFTSTGPGRAVAERFVAGDTLDSAVSVAKTLNESG
                     LTVSLDLLGEEVTDVDSAGAAVAEYLTCLDRIESEGLDANISIKPTQLGLAIDDSAAR
                     EAVTRLGRRAAAVGTTVTIDMEDSRYTEATLRLFELGQSETANFGVALQTYMKRTPHD
                     LERLIPLGGHIRLCTGAYVEPEEVALQSKVLVDAAFEQQLRTLMAADTVVPAIATHDS
                     KLLDLARDLAPGRTAPFEFQMLYGVRKDVQRELVAAGYPLRIYLPFGSQWYPYLTRRL
                     AERPANAWFLARALFSRS"
     gene            complement(1367..2179)
                     /gene="proC"
                     /locus_tag="VFZ80_05815"
     CDS             complement(1367..2179)
                     /gene="proC"
                     /locus_tag="VFZ80_05815"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019666514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX6286983.1"
                     /translation="MSDRSTIAVIGVGAMGEALVAGLLGAGWEPGDLALCVRRLDRRD
                     ELAHRTGCAVGLDLLETIAGRDVIVVAVKPRDVAEVLKTIAGEVGEDQTVISLAAGVT
                     TQAYETQLGPVPVVRAMPNTPALVREGVTGIAPGSHARAHHLGVAQEILGAVGSVRVM
                     EETLLDAVTAVSGTGPAYVFLLAEALTEAAMREGLPRDVAESFVHQTIRGAGHLLTDT
                     NKSPAQLRYEVTSPGGTTAAAMHVLEEKGFRAVVEDAVRAAAIRARELGGGA"
BASE COUNT          397 a          714 c          711 g          380 t
ORIGIN      
        1 ggtcgggccc gatcagggtc tcgaaatcgg accggtcact tggcaccaga ggatggtatg
       61 ccggatgtca gtcctcgaag gtgatctcca ccatcggaga tgtggcgacc aggtgatcca
      121 actccgcctg ggtcgtgtac cacgaggtgg aagagtgcgt gaagatgcct ctcctcacgt
      181 ccgggtcgtc gatcggtgcc gcccttgctg caatgtcgcg gccggccacg tggatcgtga
      241 cgttcgggtt ggcgagaaca tttgccagcc aatcacgttt cccgggggtg ccggttatga
      301 cgaagcgtcc gccgacctga aaccaccaga tctcgatggt tcgcggcatc ccgctcttgc
      361 ggccgatggt ggtcagatcg atcgtgcgat gtcgggcaag ttcttcatga tccgcaatca
      421 cggccgacag ggtacgactt cgagtgcagt tgaagtcaag accgggaaaa cagggccctt
      481 gccaggaacc aggcgttggc cggtcgctcg gcgaggcgtc gagtgaggta gggataccac
      541 tgcgacccaa acggcagata gatgcgcagg ggatacccgg cggcaaccaa ctccctttgc
      601 acgtccttgc ggacgccgta cagcatctgg aactcgaacg gtgctgtcct ccccggggcg
      661 agatcgcggg cgaggtccaa cagcttcgaa tcgtgggtgg caatggctgg aaccaccgtg
      721 tcggcggcca tcagcgtcct caactgttgc tcgaaggcgg catccacgag caccttggac
      781 tgaagggcga cctcctccgg ctcgacgtag gcacccgtgc agagacgtat gtgaccgccg
      841 agcggaatca gccgctccag atcgtgtggg gtgcgcttca tgtaggtctg cagcgccacc
      901 ccgaagttgg cggtttcaga ctggcccagc tcgaacagac gcaatgttgc ctccgtgtat
      961 cgggagtcct ccatgtcgat ggtgaccgtc gtgccgacgg cggcggctcg cctgcccaga
     1021 cgcgtcacag cctcccgggc cgccgagtcg tcgatggcca gaccgagctg cgtcggcttt
     1081 atcgagatgt tggcgtcgag gccctccgac tcgatgcgat cgagacaggt caggtattcg
     1141 gccaccgcgg caccagccga gtcgacgtcg gtgacctcct ccccgagcag atcgagggag
     1201 accgtgagcc cgctctcgtt gagggtcttc gccaccgaaa cggcgctgtc gagagtgtca
     1261 ccggcaacaa agcgctccgc caccgcgcgg ccgggaccgg tcgaggtgaa cagcttctcg
     1321 atgggggctg aattggccac ggcgaccgtg gcccgcgaga agagactcag gcgccgcctc
     1381 cgagctcgcg cgctctgatc gcggccgcac gaaccgcgtc ttcgacgacc gccctgaagc
     1441 ccttctcctc gaggacatgc atggcggcgg cggtggtccc gccaggcgag gtgacttcgt
     1501 agcggagctg cgccgggctc ttgttcgtgt cggtgaggag atggccggcc cctcggatcg
     1561 tctgatgaac gaaactctcg gcgacgtctc tcggcagtcc ctcccgcatc gcagcctcgg
     1621 tgagagcctc ggccaggaga aagacgtacg ccgggcctgt ccccgacacc gcggtcaccg
     1681 cgtcgagcaa tgtctcctcc atcacccgga ccgacccgac cgccccgagg atctcctggg
     1741 caactccgag atgatgggcg cgagcgtggc tccccggcgc aattcccgtc actccctccc
     1801 tgaccagtgc cggggtgttg ggcatggccc ggaccaccgg gaccggaccg agttgagtct
     1861 cgtacgcctg tgtggtgacc cctgcagcaa gtgagatgac cgtctgatcc tccccgacct
     1921 cgccggcgat ggtcttcaag acctcggcga catcacgggg tttcacagcg acaactatca
     1981 catcgcgtcc tgcgatggtc tcgaggagat cgagaccgac tgcacagccg gtccggtgag
     2041 ccaactcatc gcgacggtcg aggcgacgaa cgcacaaagc caggtcccca ggctcccaac
     2101 cagctccgag gagaccggcg acgagggcct ctcccatggc accgactcca atcacggcga
     2161 tggtcgacct gtcagacatg ggtccagatt ctaggaatgg gt
//
LOCUS       DASUTA010000294         2625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1382174, whole genome shotgun sequence.
ACCESSION   DASUTA010000294 DASUTA010000000
VERSION     DASUTA010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382174"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /gene="rpiB"
                     /locus_tag="VFZ80_05820"
     CDS             <1..535
                     /gene="rpiB"
                     /locus_tag="VFZ80_05820"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX6286984.1"
                     /translation="DGVVDSAEGQQAQPGSEATPVMPSGGEGIAIGADHGGYPLKERI
                     AFKLKEAGWELTDCGTDSHETVDYPDFAHAVAKKVADGECRWGIVIDGAGIGSSMVAN
                     KVPGVRAALCYDISSARNSREHNHANVLTLGAGLIGEGLAWQIVEEWLATEWGGGRHA
                     RRVSKIDSIEKQYSRPG"
     gene            696..1379
                     /gene="deoC"
                     /locus_tag="VFZ80_05825"
     CDS             696..1379
                     /gene="deoC"
                     /locus_tag="VFZ80_05825"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017813539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX6286985.1"
                     /translation="MPLDLAKYIDHTLLGADATAADIDRLCDEADRYRFASVCINPTW
                     VKRAANNLRGTGVPVCTVIGFPLGATTPEVKAMEARKALRDGAREVDMVINIGALKSG
                     DHDLVYDDIVKVVDAAHEVGAICKVILETALLTDEEKVVASALAKRARADFVKTSTGF
                     GPGGATVYDVALMRETVGPEMGVKASGGVRTADDAEDMIAAGATRIGASAGVQIVSGN
                     DGGDSGERY"
     gene            1351..1992
                     /locus_tag="VFZ80_05830"
     CDS             1351..1992
                     /locus_tag="VFZ80_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019487259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286986.1"
                     /translation="MEEIVAKGIELRSYAFLDSLQPQYAAFLGTVAQGFLPLAGDASL
                     WIEVAPGIEINRLTDVALKATTVKPGLQIIERYYGVLEVHSPDQAETRAAGEAILDSI
                     EHTEDDRIKPRILSSQIIRRLDDHHAQLINRTRHGQMIIPGQTLYVLECEPAAYASLA
                     ANEAEKAAEINVLEVRSFGSMGRIYLGGEERDIDVGWQAAVAALEGLTGRDST"
     gene            2032..2334
                     /locus_tag="VFZ80_05835"
     CDS             2032..2334
                     /locus_tag="VFZ80_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HEX6286987.1"
                     /translation="MAEALGMIEARSFPAMVEAADAMVKAAKVELVSMEKTGGGYVTA
                     IVRGDVAAVKAAVDAGVRGAEKVGEVTATHVIARPHVNVDLTLPLGRQEQAQDDLE"
     gene            2349..>2625
                     /locus_tag="VFZ80_05840"
     CDS             2349..>2625
                     /locus_tag="VFZ80_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX6286988.1"
                     /translation="MLLGRVAGTLVATRKEALMSGMKFLVVRQIDEDNSETGSYVVAV
                     DAVGAGPGEVVLYASGSSARQTEVTRDKPCDAVIMAIVDSWDVGGEVR"
BASE COUNT          521 a          763 c          900 g          441 t
ORIGIN      
        1 cgatggtgtt gtggattcgg ccgagggtca gcaggcccag ccgggctccg aagccacgcc
       61 tgtgatgcca tccggagggg agggaatcgc catcggagcc gaccacggcg gttaccccct
      121 gaaggagagg atcgcgttca agctgaaaga agccggttgg gagctgaccg actgtggcac
      181 cgactcccac gagaccgtcg actatcccga cttcgcccac gccgtggcaa agaaggtggc
      241 agacggagag tgccggtggg ggatcgtcat cgacggtgcg gggatcggct cctcgatggt
      301 ggccaacaag gtgcccgggg tcagggcggc cctgtgttac gacatctcct cggctcgaaa
      361 cagccgtgag cacaaccacg ccaacgtgct cactctggga gcgggcctga ttggcgaagg
      421 gctagcgtgg cagatcgtcg aagagtggct ggcgacggag tggggaggcg gtcgtcatgc
      481 caggcgggtc tccaagatcg actcgataga gaagcaatac tcgaggccgg gatgaaggca
      541 caacgatgaa cagagaggcc ctggtcgagg cgatcacccg cgaggtgctc gccgccctct
      601 ccactcgcta cgacatggct gagacgggag acgaggtgcg ccaggtcgtg gccaatggcg
      661 cagaccgcgt tgccttccac ggtgaggccg ccgacgtgcc gctcgatctc gccaagtaca
      721 tcgatcacac tctcctggga gccgatgcca cagccgcaga catcgaccga ctctgcgacg
      781 aggccgaccg atatcggttc gcctcggtct gcatcaaccc gacatgggtg aaacgcgctg
      841 ccaacaacct gcgggggacc ggcgtgccgg tgtgcacggt catcggattc cctctcggcg
      901 ctaccacccc ggaagtcaag gcgatggagg cacgcaaggc cctccgcgac ggggcccgcg
      961 aggtcgacat ggtgatcaac atcggcgccc tcaagtcggg tgatcacgat ctcgtgtatg
     1021 acgacatcgt gaaggtcgtg gatgcggcgc acgaggtggg tgccatttgc aaggtcattc
     1081 tcgagacagc tctcctcacc gatgaggaga aagtggtggc atcggcgctg gccaaacggg
     1141 ccagggcaga ttttgtgaag acatccaccg gattcggtcc cgggggtgcc accgtctacg
     1201 acgtggcact gatgcgagag acggttgggc ccgaaatggg agtgaaagcc tcgggcgggg
     1261 ttcggaccgc cgacgacgcc gaggacatga ttgctgccgg cgccaccagg atcggggctt
     1321 cggcgggagt tcaaatcgtc agcggtaacg atggaggaga tagtggcgaa aggtattgag
     1381 ctcaggtcct acgcgttcct ggacagcctt cagccgcaat acgcggcatt tctggggacc
     1441 gtagcccagg ggttccttcc cctcgcaggc gatgcttccc tctggatcga ggtagctcct
     1501 ggcatcgaga tcaaccgtct caccgatgtc gcattgaagg cgacaacggt caagcccggc
     1561 cttcagatca tcgagcgtta ctacggggtt ctcgaggtgc actcacccga ccaggccgag
     1621 acccgtgccg ccggcgaggc gatactcgac tcgatcgagc acaccgaaga cgatcggatc
     1681 aagccccgga tcctcagctc gcagatcatc aggagactcg atgatcacca tgctcagctg
     1741 atcaaccgca cccgtcacgg gcagatgatc atccccggcc agactctcta tgtcctcgag
     1801 tgtgagccgg ctgcctatgc gtcgctggca gctaacgagg ccgagaaggc cgcggagatc
     1861 aacgtcctcg aggtgcggag ctttgggtcg atgggcagga tctacctcgg tggcgaagag
     1921 cgggatatcg atgtcggctg gcaggccgcg gtggcggccc tcgagggtct gaccggtcga
     1981 gactccacgt gatgacaatg ttccagagga acgaacgaaa ggaggagctt catggctgaa
     2041 gctcttggaa tgatcgaggc ccggagcttt ccggcgatgg tcgaggcggc tgatgccatg
     2101 gtcaaggcgg ccaaggtcga gctcgtgagc atggagaaga caggtggtgg ttatgtgacc
     2161 gccatcgtgc gtggcgatgt ggccgcggtg aaagctgccg tggacgcagg cgttcgcggc
     2221 gccgagaagg ttggcgaagt caccgccacc cacgtcatcg cacgtcccca tgtcaacgtg
     2281 gacctcactc ttccgctggg ccgtcaggag caggctcagg acgacctcga gtaggaggcc
     2341 ctccactcat gctgttaggc agggtcgccg gaactctggt ggctacgcgc aaagaggccc
     2401 tcatgtcggg gatgaagttc ctcgtggtca gacagatcga cgaggacaac tccgagacag
     2461 gctcatacgt ggtggcagtg gacgccgtgg gcgccggtcc cggggaggtg gtgctctacg
     2521 cgtcggggag ctcggcacgt cagacagagg tcacccggga caagccttgc gatgccgtga
     2581 tcatggcaat cgtggactct tgggacgtcg gtggcgaagt caggt
//
LOCUS       DASUTA010000295         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_86487, whole genome shotgun sequence.
ACCESSION   DASUTA010000295 DASUTA010000000
VERSION     DASUTA010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86487"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..793
                     /locus_tag="VFZ80_05845"
     CDS             185..793
                     /locus_tag="VFZ80_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286989.1"
                     /translation="MAVNPDPKPGRWILPLVILGMIAFTYFFVRELPEASTETSLVSG
                     PDTTTTLGGGGDGSTSTTQSQTPLDPETQAYLDTIDTINEDLQVQRTELVTVNEGFNA
                     DPREIEFDDAETRFEAVTTETQALADQLGGLTPPAALEGNHASLQAAVDLAAVSATEA
                     LAGLRSTDTGERRNTAVDSYVQAATDFGTEVTNTHNAAESNA"
     gene            complement(832..>2223)
                     /gene="glnA"
                     /locus_tag="VFZ80_05850"
     CDS             complement(832..>2223)
                     /gene="glnA"
                     /locus_tag="VFZ80_05850"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX6286990.1"
                     /translation="VENEGYEFVDLRFCDLPGQVQHFTVPVHQLTEDSFDEGFGFDGS
                     SIRGFQDIQESDMILIPDADTAVEDVFRMRKTLILYCFVNDPVTGTPYEKDPRYVAKK
                     AEDYLVSTGIADTSFWGPEAEFYIFDDVRFGQNQNSGYYFVDSIEGAWNSGEFEEGGN
                     LGYKPRYKEGYFPVPPTDHYQDLRSEMTARLEAAGIEIEVQHHEVGTAGQAEIDMKYD
                     TLLRTADNVTLYKYIVKNTAWEMGKSVTFMPKPIFEDNGSGMHTHQSLWRDGEPLMYD
                     ESGYAGLSDLARWYIGGLLKHAPAVLAFAAPTTNSYHRLVPGYEAPVNLVYSSRNRSA
                     AVRIPQYSQSPAAKRLEFRCPDPSCNPYLAFSAMLLAGLDGVQNQIEPHDPVDKDIYD
                     LPPAELEGLPSVPGSLPEALAALEDDQEFLLQGGVFTEGLIESWIAYKQEHEVDPVRL
                     RPHPYEFHLYYDI"
BASE COUNT          412 a          703 c          700 g          408 t
ORIGIN      
        1 aacggcgtat accagccatt gacgattccc acccttgggc tcgagtgtct cccaggggat
       61 ccgctcgtat acctcgtctc gttccggctc catgatctga acgtatggaa tgccgaatgg
      121 tgcttcaagg gggttggtac agaaatcagg gaaccgaacg ggccttggct atcgtcattc
      181 ggtaatggca gtcaaccccg acccgaaacc gggccgttgg atcctccctc tggtcatcct
      241 ggggatgatc gcgttcacct acttcttcgt gcgcgaactg cccgaggcct ccaccgagac
      301 ctccctcgtg tcgggccccg acaccactac gacgctgggc ggtggcggcg acgggtcgac
      361 gagcaccacc cagtctcaga cacccctcga tcccgagacc caggcttacc tcgacacgat
      421 cgacacgatc aacgaggacc tgcaagttca gcgcaccgag ctcgtcacgg tcaacgaggg
      481 cttcaacgcc gacccccgcg agatcgagtt cgacgatgct gagaccaggt tcgaggccgt
      541 gactacagag actcaggcgc tggccgacca gctcggcggt ctgacacccc ctgctgcctt
      601 ggagggaaac cacgcctctc tgcaggcggc agtcgacctc gcagcggtca gtgctaccga
      661 ggcgcttgcc ggcctgcgct ccacggacac cggcgaacgg cgcaacacag cagtcgactc
      721 ctatgtgcag gcagccacgg acttcggtac cgaggtcacc aacacccaca acgccgccga
      781 gtcgaacgcg tagaacgcgg ggtgctgctg ccgcagcacc ccgacgttca cctagatgtc
      841 gtagtacagg tggaactcgt aggggtgagg ccggagacgg accggatcga cctcgtgctc
      901 ctgcttgtag gcgatccatg actcgatcag gccctcggtg aacaccccgc cctgcaacag
      961 gaactcctgg tcgtcctcca gggctgcgag cgcctcgggg agactccccg gtacggaagg
     1021 aagcccctcg agctcagccg gcgggaggtc gtagatgtcc ttgtcgaccg ggtcgtgcgg
     1081 ctcgatctgg ttttggaccc cgtcgagacc ggcgaggagc atcgccgaga acgccagata
     1141 tgggttgcac gaagggtccg ggcaacggaa ctcgagccgc ttcgccgccg gcgactgcga
     1201 gtactggggg atgcggacgg cggctgagcg gttgcgactg gagtaaacca ggttcaccgg
     1261 agcctcgtag cccgggacca ggcgatggta cgagttcgtc gttggtgccg cgaacgccag
     1321 gaccgcaggg gcgtgcttga gcagcccacc gatgtaccac ctggccagat cggagagccc
     1381 ggcgtaacca ctctcgtcgt acatgagggg ctcgccgtct ctccacagcg actggtgggt
     1441 gtgcatgccc gacccgttgt cctcgaagat cggcttgggc atgaacgtca ccgacttgcc
     1501 catctcccat gccgtgttct tcacgatgta cttgtagagc gtcacgttgt ccgcggtgcg
     1561 gagcagagtg tcgtacttca tgtcgatctc ggcctggccc gcggtgccca cctcgtggtg
     1621 ctgaacctcg atctcgatgc cagccgcttc gaggcgtgcg gtcatctcgg agcgaaggtc
     1681 ctggtaatgg tccgtcggag ggacggggaa gtagccctct ttgtaccggg gcttgtagcc
     1741 gaggttcccg ccctcctcga actcacccga attccaggcg ccctctatcg agtcgacgaa
     1801 gtagtagccg gagttctggt tctggccgaa acggacgtcg tcgaagatgt agaactctgc
     1861 ctcgggtccc cagaatgagg tgtcggcgat gccggtggac accagatagt cctccgcttt
     1921 cttggcgacg tagcgagggt ccttctcata cggggtgccg gtgaccgggt cgttcacgaa
     1981 gcagtagagg atgagggtct tcctcatcct gaacacgtcc tcgaccgcgg tgtcggcgtc
     2041 gggtatcagg atcatgtcgg attcctggat gtcctggaac ccgcggatgg acgagccgtc
     2101 gaagccgaag ccctcgtcga aagagtcctc ggtcaattga tgaaccggca cggtgaagtg
     2161 ctggacctgg ccgggcaggt cacaaaagcg caggtcgacg aactcatagc cctcgttctc
     2221 gac
//
LOCUS       DASUTA010000296         3891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1510861, whole genome shotgun sequence.
ACCESSION   DASUTA010000296 DASUTA010000000
VERSION     DASUTA010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510861"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VFZ80_05855"
     CDS             complement(<1..417)
                     /locus_tag="VFZ80_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6286991.1"
                     /translation="MRARGLAVTYGTTVALAPSDFEIPAGAVTAVIGPNGSGKSTLLN
                     AVAGLVEPSAGVIEVDVEPGRLSYVMQATKINENLHVTVREVVAMGRFAALGPYRWMR
                     EDDKAAVESALDRLGIRDLATRHLHDLSGGQRQRALV"
     gene            complement(429..869)
                     /locus_tag="VFZ80_05860"
     CDS             complement(429..869)
                     /locus_tag="VFZ80_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX6286992.1"
                     /translation="MTKSTVDRQVELRLLEHGVRYTKGRNAVVESLARASGPLSASEL
                     SDELSPDVPLSSIYRTLSVLEDAGVVAHHLGAKGLTRFELAEWLTGHHHHLVCVQCGS
                     VSDVDIPPSQEESVRSLVSQIAALAEFEATDHALEIEGRCSKCA"
     gene            903..1877
                     /locus_tag="VFZ80_05865"
     CDS             903..1877
                     /locus_tag="VFZ80_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX6286993.1"
                     /translation="MITILIPDKNPRTRRRTVTGLLLGLTIVLAACGSPGTGGADDLT
                     IVATTSIWGDVVSQLVGDDAEVEVLIPRGSDPHDYQTSSSQVATLHAADLVIANGLGL
                     EEGLHDVLESAKSDGVAVFEVGPLLDPLPFSGEDDHEGDEESEESHDEDPHVWLDPQR
                     VSTAADFIAQRLAEIEPSVDWQARANEYVAELEQTDSQIATTLAAVPDSDRKLVTNHD
                     ALGYFADRYGFEVIGVVIPGGSTLSDPSSAALAALVAEIEHEGVHAIFAETTQPTRLA
                     EAVAAEVGSDVSVVELYTGSLGEPGSGAETLVGMLLTDTTLIADALAR"
     gene            1898..2743
                     /gene="aztB"
                     /locus_tag="VFZ80_05870"
     CDS             1898..2743
                     /gene="aztB"
                     /locus_tag="VFZ80_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012689473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease AztB"
                     /protein_id="HEX6286994.1"
                     /translation="MDWLIAPFELAFQQRALTAGLLAAVALALVGTWVVIRGMSFLGD
                     ALVHGVVPGIAVALLLDLNVLLGAVVAAVVMLLGINLIHRQTTFSEDTGIGLLFVGML
                     GLGVIIISRTDSYSGDLTAILFGDALGVTIDDIWLLVGLVTVTLIVSIVLYRPFVALA
                     FNEQKAELLGMRPRLAHAALLGLITLAIVGSFQTVGTLLVFGLLVGPAATAALLVRRV
                     PAMMVVAALIGVVSVVTGLIVSYHANTSGSATMGVTPIVLFFVVLATKGLRKRLPPAP
                     TPTNG"
     gene            2816..>3891
                     /locus_tag="VFZ80_05875"
     CDS             2816..>3891
                     /locus_tag="VFZ80_05875"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX6286995.1"
                     /translation="MLRRLTTLLGLVVAVLVILGSSPSSAELPPGGTFIDDDGNVHEG
                     SIEAVFAAQITSGCDERGIRFCPDLVVTRGQMAAFLARALNLAPASEDYFTDDDGSTF
                     EGAIDKIAEAGITVGCNPPDHDEYCPDKRVTRAEMATLLVRAFPELLLETAPDVFTDD
                     GDSVHHTNINRIAAERITLGCNPPDNDNFCPDEGVSRAQMATFLTRALKLQAVKPPAQ
                     FPIERVSRFTTFYDCCQPRVTNIRRMAAEVDGYVVMPGETFSIDQVVGPRTSSGGYVP
                     APYLQNGQGRCCAIGGGVSQFGTTIHNAVFWGGYQIDDHRPHTGWISRYPLGIEATLV
                     YSSIDFKFTNDTVTPVYIETSSTGT"
BASE COUNT          683 a         1293 c         1195 g          720 t
ORIGIN      
        1 caccagcgct cgctgccgtt ggcccccgga taggtcatgc agatgtcgcg tggcgaggtc
       61 gcgtattccc agccggtcca gggcggactc gacggcggcc ttgtcgtcct ccctcatcca
      121 tcgataaggg cccagtgcgg cgaaccggcc catggccacc acctcccgca ccgtgacatg
      181 aagattctcg ttgatcttgg tggcctgcat cacgtatgag agccgaccgg gctccacatc
      241 gacctcgatg acgccggccg atggctcgac cagcccggcg acggcgttga gaagagtcga
      301 cttgcccgat ccgttgggcc cgatgacagc ggtgacggcg cccgcgggga tctcgaaatc
      361 cgaaggggcg agtgccacgg tggtcccgta ggtcacggcg agccctcggg cacgcacggc
      421 agtccggctc aggcgcattt cgagcagcgc ccttcgatct cgagggcgtg gtcggtggct
      481 tcgaattcgg cgagtgctgc gatctgggac accagactgc ggacactctc ctcctgtgac
      541 gggggtatgt cgacatcgga caccgagccg cactggacac agaccagatg gtgatgatga
      601 ccggtcagcc attccgccag ctcgaaccgg gtcagccctt tggcgccgag atggtgagcg
      661 accactccgg catcctcgag cacagagagt gtgcggtaga tcgaagagag ggggacatcg
      721 gggctgagct cgtcactcag ctccgacgca gacagcggcc cggaggccct ggcgagactc
      781 tcgaccacgg cgttgcgtcc cttggtgtaa cgcacaccat gctcgaggag cctcagctcc
      841 acttgacgat cgaccgtcga tttggtcaca agcccacctt aatgattatg attctcattg
      901 ccatgataac gattctcatt cccgacaaga acccgagaac cagacgtaga accgtgacgg
      961 ggttgctgct gggtctcacc atcgtcctgg cggcatgcgg aagccccggc accggcggag
     1021 ccgatgacct gacgatcgtc gccaccacct cgatctgggg tgacgtggtc agccagttgg
     1081 tgggcgatga cgccgaggtc gaagtgctca tccccagagg cagcgaccct cacgactacc
     1141 agacctcctc gagtcaggtc gccaccctgc atgcggccga tctcgtgatt gccaacgggc
     1201 tcgggctgga ggaaggcctg cacgacgtcc tcgagagcgc aaagagcgat ggcgtcgccg
     1261 tcttcgaggt cggaccactt ctcgacccgc tccccttctc cggtgaggac gaccacgagg
     1321 gagacgagga atcggaggaa tcacacgacg aggacccgca tgtgtggctc gaccctcagc
     1381 gagtgtccac ggccgcagat ttcatcgccc agcgtctcgc cgagatcgag ccgtcggtcg
     1441 actggcaagc cagagccaac gagtacgttg ccgagctcga acagacggat tcccagatcg
     1501 ccacgaccct ggccgccgta ccggattcag atcgaaagct ggtgaccaac cacgatgccc
     1561 tcggctattt cgccgacaga tacggcttcg aggtgatcgg tgtcgtgatt ccaggaggat
     1621 cgaccctctc cgacccgagc tcggcggctt tggccgcgct cgtggcggag atcgagcacg
     1681 agggcgtgca tgccatcttc gccgagacca cccagccgac gcggctcgcc gaggccgttg
     1741 ccgccgaggt gggatccgac gtctcggtcg tcgagctcta caccggatct ctcggcgagc
     1801 ccggctcggg cgccgaaacc ctggtcggga tgcttctcac cgacaccacg ctcatcgccg
     1861 acgccctcgc gagatgacct gcggttaagt tctcgatgtg gactggctga tcgccccgtt
     1921 cgagctcgcc tttcagcaga gagcactgac tgcgggattg ctggcggcgg tggccctcgc
     1981 actcgtcggg acctgggttg tcatcagagg gatgtcgttc ctgggcgacg ccctcgtcca
     2041 tggagtggtg cctgggatag ccgtggccct cctcctcgac ttgaacgtcc tcctcggcgc
     2101 ggtcgtcgcc gcggtggtca tgctgttggg aatcaacctc atccatcgcc agaccacctt
     2161 ctccgaagac acgggcatcg ggctcctctt cgtcggcatg ctcggtctcg gagtcatcat
     2221 catctcgaga accgactctt acagcggcga cctcactgcg atcctgttcg gcgacgcgct
     2281 gggggtgacc atcgacgaca tatggctcct cgtcgggttg gtgactgtca ccctgatcgt
     2341 gagcatcgtc ttgtaccggc cgttcgtcgc cctggcattc aacgagcaga aggcggagtt
     2401 gctgggcatg cgaccgagat tggcccacgc cgccctcctc gggctcatca ccctggccat
     2461 cgtgggctcc tttcaaaccg tcggcacatt gctcgtgttc ggtttgctgg tgggcccggc
     2521 cgccaccgcg gcgctgctgg tgcgtcgggt cccggcgatg atggtcgttg ccgccttgat
     2581 cggagtggtc tcggtggtca ccgggctgat cgtgagctat cacgccaaca cctcgggatc
     2641 ggcgaccatg ggagtgacac ccatcgtgct cttcttcgtg gtgctcgcca cgaagggact
     2701 tcgcaagagg ctcccgcccg ccccaacccc gaccaacggg tgacgggctc gaattcctga
     2761 gtcattccgg gcgatatcgc ttggagtagg taaccacctg tgcgacactc gcacgatgct
     2821 tcgtcgtctg accactctcc ttggcctcgt ggtggctgtc ctggtgatcc tcggtagctc
     2881 cccctcatct gcggagcttc cgccgggagg cacgttcata gacgacgacg gcaacgtcca
     2941 cgaaggttcg atcgaagccg tcttcgccgc gcagatcacc agcggatgtg acgaacgtgg
     3001 tatccggttc tgccccgacc tcgtggtcac ccgcggtcag atggcggcgt tcctggcgcg
     3061 ggcactcaat ctcgcaccgg cgtcggagga ctacttcacc gacgacgacg gctcgacttt
     3121 cgagggagcc atcgacaaga tcgccgaagc gggcatcacc gtcggctgca atccaccgga
     3181 ccacgacgag tactgcccgg acaagagagt cacccgcgcc gagatggcca ccctcctcgt
     3241 gcgggcgttc cccgagctgt tgctcgagac cgcccccgac gtcttcaccg acgacggcga
     3301 cagtgtccat cacaccaaca tcaaccgcat cgccgccgag cgaatcaccc tcggatgcaa
     3361 tccgcccgac aatgacaatt tctgccccga tgagggggtc tcccgggccc agatggccac
     3421 cttcctcacc agggccttga agctccaggc cgtgaagccg ccggcgcagt tcccgatcga
     3481 gcgagtgtca cgcttcacca ccttctacga ctgctgtcag ccccgagtga ccaacatccg
     3541 ccggatggcc gccgaggtcg acggctacgt cgtgatgcct ggtgagactt tctcgatcga
     3601 tcaggtggtc ggcccccgga cgtcctcggg tggctatgtg ccagccccct atctccagaa
     3661 tggccagggt cgttgttgtg cgatcggtgg tggtgttagt cagttcggca ccaccatcca
     3721 caacgccgtc ttctggggtg gctatcagat cgacgaccac agaccccaca ccggatggat
     3781 atcgagatat ccgctgggga tcgaggcgac gctggtgtat tcgtcgatcg acttcaagtt
     3841 cacgaacgac acggtcacgc cggtctacat cgagacatcg agcaccggca c
//
LOCUS       DASUTA010000297         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1510889, whole genome shotgun sequence.
ACCESSION   DASUTA010000297 DASUTA010000000
VERSION     DASUTA010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510889"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="VFZ80_05880"
     CDS             complement(<1..1142)
                     /locus_tag="VFZ80_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286996.1"
                     /translation="MTATVDSPGTSESSLHGEIAISVRDLKKHFPIREGALQRVGGHV
                     RAVDGVSFDIPRGETVGLVGESGCGKTTLGRCVSGLTDPTAGGVYFDLARSEHDRLDA
                     ILAVAEQDRSEQMRSDLAHLESEHRVDQLRKGAWTRYRRNCQVVFQDAFASLNPRHLV
                     REIVGRPLKVHKEASGDQLTERVVELLERVGMGRQHLYRYPHQFSGGQRQRLSIARAL
                     ALEPELIILDEPTSALDVSVQAQILNILNDLQRQLHLTYLFITHDLSVVRHMADRVVV
                     MYLGKVAEAGTTADIFDDGRHPYTQALHRASPDVEDDPDTALVTLEGSVPDPARPPQG
                     CRFHTRCPVATPVCGWEIDDVVRWLEDEPGMFDALTGITREDEFKAA"
     gene            complement(1139..>2166)
                     /locus_tag="VFZ80_05885"
     CDS             complement(1139..>2166)
                     /locus_tag="VFZ80_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6286997.1"
                     /translation="GSGCEVCAGTGRLPVACGKCAGAGCPHCNDTGFETIDLSRIPER
                     QLREIRGDRIAMIFQDPAKALNPALTVRSQIAEVFSQHRSDEMLERAGLGRTRNPLLR
                     RHVSQKTRLVERWLLALPPMRGQRRRIEQVIDGMVAAALAETQIPNPAKVMERYPHEL
                     SGGMKQRAMIAQALACNPDLLIADEPTTALDVTVQARIIELIKQLQVRHESAVLYISH
                     DLSLVRRVCDRVAVMYAGRIVEIGPTEEIFANPLHPYTRGLLAAIPSRAHARGQLVAI
                     KGTVPELIDPPPSCRFVQRCSYAVSICGEFDPELASGPGRHAVACFIHNPPEDAAELP
                     SFSRVTT"
BASE COUNT          373 a          678 c          712 g          403 t
ORIGIN      
        1 gcggccttga attcgtcctc ccgggtgatc ccggtcaatg cgtcgaacat gcccggctcg
       61 tcttcgagcc agcgcaccac gtcatcgatt tcccacccgc ataccggcgt cgccaccggg
      121 cagcgtgtgt gaaaccgaca accctggggt gggcgggccg gatcggggac cgacccctcc
      181 agagtcacca gcgcggtgtc agggtcatcc tcgacgtcgg ggctggcccg atgcagggcc
      241 tgggtgtagg ggtgtcgccc atcgtcgaag atgtccgccg tcgtgcccgc ctctgccact
      301 ttccccagat acatcaccac cacgcgatcc gccatgtggc gaaccacgga gaggtcatgg
      361 gtgatgaaca ggtaggtgag atgaagctgt ctctgaagat cgttcaagat gttgaggatc
      421 tgggcctgga cggagacgtc gagagccgag gtcggctcgt cgaggatgat cagctccggt
      481 tcgagagcca gagcgcgtgc gatggagaga cgttgtcgct gaccgccgga gaactggtgg
      541 gggtatcgat ataggtgctg gcgtcccatg cccactcgct ccagcaactc aacgacacgc
      601 tcagtgagct ggtccccaga cgcctccttg tggactttga gtgggcgtcc gacgatctca
      661 cggaccaggt gtcggggatt cagcgaggca aaggcgtcct ggaacacgac ctgacagttc
      721 ctccgatacc gggtccaggc accttttcgg agttggtcga cgcggtgttc gctctcgaga
      781 tgcgccaaat ctgagcgcat ctgctccgac cgatcttgct cggcgacggc caggatggcg
      841 tcgagtcggt cgtgctcgct tctggcgagg tcgaagtaga ccccaccggc ggtgggatcg
      901 gtgagaccgc tgacgcatcg ccccaacgtg gtcttgccac acccactctc cccgaccagg
      961 cccaccgtct cacccctggg gatgtcgaaa ctgacgccgt cgacggcgcg gacgtgtcct
     1021 cccactcgct gcaaggctcc ttcccggatt gggaagtgct tcttcaggtc tctcaccgag
     1081 atggcgatct caccgtgcag ggaggattca gaggttcccg gtgagtcgac ggtggccgtc
     1141 acgtcgtcac tctggagaac gagggaagct ctgcagcatc ctccggcggg ttgtggatga
     1201 agcacgccac ggcatgtcga cctgggccgg aggcgagctc ggggtcgaac tcaccgcaaa
     1261 tcgagactgc ataagagcag cgctgcacga atcgacacga cggcggcggg tcgatcaact
     1321 cgggcacggt ccctttgatg gccaccagtt gcccgcgggc atgggctcgg gagggaatgg
     1381 cggcgaggag gcctcgtgtg taggggtgga gaggatttgc gaagatctcc tccgtggggc
     1441 cgatctccac gatacggccg gcatacatga ccgctacgcg atcacacacc cgccgaacca
     1501 gggagaggtc gtggctgatg tacaggaccg ccgattcgtg ccgaacttgg agctgtttga
     1561 tgagctcgat tatccgggcc tgcacggtca cgtcgagcgc cgtggtcggc tcgtctgcga
     1621 tgagcagatc ggggttacag gcgagtgctt gagcgatcat cgcccgctgc ttcatcccac
     1681 ccgacagctc gtgcgggtag cgctccatca ccttcgctgg attggggatc tgggtctcgg
     1741 ccagggcggc agcaaccatc ccgtcgatca cctgctcgat ccgccgacgt tggccccgca
     1801 tcggggggag ggcgagcaac cacctctcga cgagccgtgt cttctgggac acgtgccgtc
     1861 gcagcagcgg gttgcgcgtt ctccccaggc cggctcgctc cagcatctca tcactgcggt
     1921 gttgggaaaa gacctccgca atctggctgc ggacggtgag cgccgggttc agggccttgg
     1981 cggggtcctg gaagatcatc gcgattcgat cgccccgtat ctcccgtagt tgtcgttcgg
     2041 ggatccggct gaggtcgatt gtctcgaagc cggtgtcgtt gcagtgagga catccggccc
     2101 cggcgcactt tccgcatgca accggcagac gcccggtgcc cgcacacact tcgcatcccg
     2161 acccag
//
LOCUS       DASUTA010000298         2493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1640932, whole genome shotgun sequence.
ACCESSION   DASUTA010000298 DASUTA010000000
VERSION     DASUTA010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640932"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..740
                     /locus_tag="VFZ80_05890"
     CDS             165..740
                     /locus_tag="VFZ80_05890"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6286998.1"
                     /translation="MSTRSILVLVVSALFVAGCGGGDGGATTTEAPADTPITTTQSPA
                     TTGAEMTTTTAGDETSTTEAQMMDGVHASETDLGTILVDAEGFTLYIFTNDTNGTSTC
                     YETCAATWPPVPGDITISSDLDASIFGTTTRDDGSEQLTVNGMPLYLYAPDTEPGDTA
                     GQGLGGVWFVVDAEGEVIEASAGGRRIDYGY"
     gene            781..1431
                     /locus_tag="VFZ80_05895"
     CDS             781..1431
                     /locus_tag="VFZ80_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020556473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX6286999.1"
                     /translation="MDLTEALYTTRAMRRVGDKPIPDEVKARILDAAIRAPSGGNSQN
                     WRMVVIDDAGVRSRLGPLYREAYRQLQETFYAGRREAAEKTGDEAALRVMRSSDWLAE
                     NFESVPMWVLFFSRNDPTGSSIYPAVWSAMLAARGEGVGTCLTTILGNFKSAEVFEML
                     DIPTDKGWEFKAAVSCGYPTGRWGLAQRAPAEKVAYSNRWGQPLDFAVDGPMWSEN"
     gene            1546..>2493
                     /locus_tag="VFZ80_05900"
     CDS             1546..>2493
                     /locus_tag="VFZ80_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX6287000.1"
                     /translation="MASESLTSRPPSAWWLVVTVITWALAGGCLVAAIVHQDSATAPI
                     GEGEVFVDDAATASALISESSSLDQGVRRARNSLAVEAVSVVASDGRVLASTSTTLDD
                     AVITNPLLVYGLADGRFTALANPIAYRIEIDGVEEWPRDSILYEVLAPRADDETSVLL
                     HYDVSELLSRRTRPGEIQPLTIQLLALGGVFALLGTAVFVGQTRAARRHREMSIESEL
                     LREQSRQLEEANTELAEARRRAERALALAEEKMRIRSEFVLMINHELRTPLTSVVTGA
                     ELMQDGSLGEDDRQELIDSMIAHGRRLNEIIDQILAVARI"
BASE COUNT          485 a          791 c          774 g          443 t
ORIGIN      
        1 cggaacgttg ccgggccgac cacgagatcc atggggccca aacgcccctt ccaggtgatg
       61 gtctcaacag tcatggtgaa accttagctg tgtaccccaa acccgcgttc acgaaccgtc
      121 ccgagagctc gtgcgtagat cccaggagaa cacccaggag acccatgagc acacgttcca
      181 tactcgtcct ggtcgtttcc gccctctttg tcgcagggtg cgggggaggc gacggagggg
      241 caacgacgac cgaggcccca gctgacactc ccatcacgac gacccaatcc cccgcgacca
      301 ccggggcaga aatgaccacc acgacggccg gtgatgaaac ctccacgacc gaggcgcaga
      361 tgatggatgg cgtccacgcc tccgaaaccg atctgggaac gatcctcgtc gacgccgaag
      421 gattcaccct ctacatcttc accaacgaca ccaacggcac cagcacctgt tatgagacct
      481 gcgccgccac atggccaccg gtgccgggtg atatcacgat ctcatctgat ctcgacgcgt
      541 cgatcttcgg aaccaccacc cgggacgatg ggagcgagca actgacggtc aatggcatgc
      601 cgctctatct gtatgcgccc gacaccgagc ccggagacac ggcggggcag ggtctcggcg
      661 gtgtttggtt cgtggtcgat gccgaaggcg aggtcatcga ggcctcggct ggaggtcggc
      721 gcatcgacta cggctactga cgaggggtca gccgtgagcc ggacttcgta ggctggccac
      781 atggatctca ccgaagctct ctacacgacg cgcgcgatgc gccgcgtcgg cgacaagccg
      841 attcccgacg aggtgaaggc ccgcatcctc gacgcagcca tcagggcacc gtcgggcggc
      901 aactcccaga actggcggat ggtcgtgatc gacgatgccg gggtgcgcag ccggctcggt
      961 ccgctctacc gcgaggcgta ccggcagttg caagagacct tctatgcggg acgtcgggaa
     1021 gctgctgaga agacgggcga cgaagcagcg ttgcgggtga tgcgatcctc tgattggctg
     1081 gccgagaact tcgagtcggt cccgatgtgg gttctcttct tcagccgtaa cgatccaacg
     1141 ggctcatcga tctacccggc ggtgtggagc gccatgctgg cggcgcgtgg agagggcgtc
     1201 ggcacatgtc tcaccaccat cctgggcaac ttcaagagtg ccgaggtgtt cgagatgttg
     1261 gacatcccca ccgacaaggg gtgggagttc aaggccgccg tgtcgtgcgg gtacccaacc
     1321 ggaaggtggg gcttggctca gcgggctcca gccgagaagg tcgcctactc aaatcggtgg
     1381 ggtcaacctc tcgacttcgc cgtcgatggg ccgatgtgga gcgagaacta gaaggaggag
     1441 acctcctagg acagcaacgc gcgtctcgaa aacctccaat ctcgctcaca acgattgagc
     1501 cgctaggtcc ttacaggaat taggtgactc cgccgatatt ctcgcgtggc gtctgaatct
     1561 ctcacctcgc gcccgccatc ggcatggtgg ctggtggtca ccgtgatcac ctgggcgttg
     1621 gctggtggtt gcctggtcgc ggccatcgtc caccaagact cggcaaccgc tcccattggc
     1681 gaaggtgagg tatttgtaga tgatgccgcg actgcttccg ctctcatcag cgagagctcc
     1741 tctctggatc aaggggtgcg gagggcacga aacagcctcg ccgtcgaggc cgtctctgtc
     1801 gttgcttccg atgggcgagt gcttgcctcg acctccacca cgctcgacga cgcagtgatc
     1861 accaacccgc tgctcgtcta tgggctcgcc gacggccgat tcactgcatt ggccaacccg
     1921 atcgcgtacc gaatcgagat cgatggcgtc gaggagtggc ctcgcgacag catcctctac
     1981 gaggtcctag cgccacgggc cgatgacgag acgtccgtac ttctccatta cgacgtgtcg
     2041 gagcttctct ccagacggac ccgtccaggt gagatccaac cgctgacgat ccagctgctc
     2101 gctctcggcg gtgtcttcgc cctcctcggc actgctgtct tcgtcggaca gactcgcgcc
     2161 gctcgtcgcc accgcgagat gtcgatcgag tccgagctgc ttcgcgagca atcccgccag
     2221 ctcgaggaag ccaataccga gctggcggag gcgagacggc gagcagaacg tgcattggcc
     2281 ctggccgagg agaagatgcg aattcgttcc gagttcgttt tgatgatcaa ccacgagttg
     2341 aggaccccgc tcacctcggt ggtcacgggc gccgagttga tgcaggacgg aagcctcggc
     2401 gaagatgacc gtcaggagct catcgactcg atgatcgccc acggcagacg actcaacgag
     2461 atcatcgacc agatcctggc ggtagcacgg atc
//
LOCUS       DASUTA010000299         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1511150, whole genome shotgun sequence.
ACCESSION   DASUTA010000299 DASUTA010000000
VERSION     DASUTA010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511150"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFZ80_05905"
     CDS             <1..439
                     /locus_tag="VFZ80_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6287001.1"
                     /translation="LYLGWMLTDNAKAADILDRDLIGFLTAVNESGQPQTAPVWFWRD
                     GDDLVVYNRPNSPRLTSIAANPKVSFSVRGDIRAAGALILEGTAAIGGLPAAVDIPEY
                     VAKYESEIERLGWTPEVFSDMYSEGVRITVTRVWAWGLDALGQ"
     gene            496..927
                     /locus_tag="VFZ80_05910"
     CDS             496..927
                     /locus_tag="VFZ80_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019521742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEX6287002.1"
                     /translation="MALESTGRTHWEGDLFSGSGTTSFDSGAAGPLPVTWAARAEEHG
                     SVTSPEELIAAAHSACYSMAFSNGLAKNETPPTSLDVTATATFVPGTGITTMVLDVVG
                     KVDGIDEEKFLELAEAAKNGCPVSQALAGNVEISLNASLAS"
     gene            complement(961..1506)
                     /locus_tag="VFZ80_05915"
     CDS             complement(961..1506)
                     /locus_tag="VFZ80_05915"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEX6287003.1"
                     /translation="MDFSHYTTKPVELAVSLTNTKGIIKDELENFDQLVEFLDGYKEL
                     WEGVAKPPNPSDLEAVQELRASLREVIESEDENTAAKKVNVLLESYGAIPRISTHSGS
                     PHLHFEPTETSIVCWLGAITAMGLASVIVEHGVERFGVCDSTTCIDVYVDTSRNRSRR
                     HCSNTCSTREAVAAYRKRKTD"
     gene            1567..1755
                     /locus_tag="VFZ80_05920"
     CDS             1567..1755
                     /locus_tag="VFZ80_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287004.1"
                     /translation="MKSLRHEMVNAMLDLAKSIQEDRRHEADIQRIVSNLPRERSLSV
                     GRYRVTVSRRAGRTLPAA"
     gene            1789..1995
                     /locus_tag="VFZ80_05925"
     CDS             1789..1995
                     /locus_tag="VFZ80_05925"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ family domain-containing protein"
                     /protein_id="HEX6287005.1"
                     /translation="MPTGQPDSVETHADRLIREAMEAGRFDDLPGVGEPIPGAGSKDD
                     AGWWIRGWLERNRANGDQGASNSE"
BASE COUNT          410 a          637 c          677 g          400 t
ORIGIN      
        1 gctctacctt gggtggatgc tgacagacaa cgccaaggcc gcggacattc tcgatcgaga
       61 tctcatcggc ttcctgaccg ccgtcaacga gtccgggcaa ccgcagaccg ctccggtttg
      121 gttctggcgg gacggcgacg acctcgtcgt ctacaaccgc cccaacagcc ccagactgac
      181 ctcgattgcg gccaatccga aagtctcgtt cagtgtgcgc ggtgacattc gcgccgccgg
      241 cgcactgatt ctggagggga ccgcggcgat cggtggtctg cccgccgcag tcgacatccc
      301 ggagtacgtg gccaagtacg agagcgagat cgaaagactc ggttggactc cggaggtttt
      361 ctctgacatg tatagcgaag gtgtccggat cacggttacc cgcgtctggg catgggggct
      421 cgacgctctg ggccagtgag gggtaggcgt cggcgccggt agtgtcggag gcaatcgcaa
      481 acagaaaggt ctgagatggc tctcgagagc accggtcgca cccattggga gggcgatctc
      541 ttcagcggct ccggcacgac cagcttcgat agcggcgcgg ccgggcccct tcccgtcacc
      601 tgggcggcac gtgccgagga gcatggttcg gtcaccagcc cggaggagtt gatcgcggcg
      661 gcgcactcgg cgtgctactc gatggcgttc tccaacggcc tcgccaagaa cgagacacca
      721 ccgaccagcc tcgacgtgac ggcgacggcc acgttcgtgc ccggcaccgg gatcacaacg
      781 atggtcctcg atgtcgtcgg caaggtagac ggaatcgacg aggagaagtt cctggaactg
      841 gcagaggctg cgaagaacgg atgcccggtg tctcaagccc tcgccggcaa tgtggagata
      901 tcgctcaacg ccagcctggc gagctagatc gccgcaccat agtcacggct acaccgtgac
      961 tcagtccgtc ttccgcttgc ggtaggcggc cacagcctct ctggtagagc aggtgttcga
     1021 gcagtgacgt ctcgaacggt ttcgcgaggt gtcgacgtag acgtcgatgc acgtcgtgga
     1081 gtcgcagaca ccgaaccgct cgactccgtg ctccacgatc acgctggcca gacccatagc
     1141 cgtgatggcg ccgagccagc acacgattga tgtctccgtc ggttcgaagt ggagatgggg
     1201 agacccgcta tgtgtcgaga tccgcgggat cgctccatag gactcgagca gtacgtttac
     1261 cttcttggcc gccgtgttct cgtcctccga ctcgatgact tcacggagcg aggcgcgtag
     1321 ttcctgaacc gcttcgagat cggaggggtt aggaggtttg gccacgcctt cccacaactc
     1381 cttgtagccg tcgaggaact cgaccagttg gtcgaagttc tccagctcgt ctttgatgat
     1441 ccccttcgtg ttggtcaggg aaacggctag ctcgacgggc ttggttgtgt aatgagagaa
     1501 atccatttga ctcgttacgc cgatctgtta ctgtaatgag tgtaagcggt aatgcatctt
     1561 accgcaatga aaagtctcag acacgaaatg gtgaacgcca tgttggacct agcaaagagc
     1621 attcaagaag accgccggca tgaggccgac atccagcgaa tcgtctccaa cctgcccagg
     1681 gagcggtcgt tgtcggtggg tcgctaccga gtcaccgtca gtcgacgggc cggtaggacc
     1741 ctgccggcgg cctagtgagt cgggaacggc cggtagggtc cttcctccat gcctaccggc
     1801 caacccgatt ccgttgaaac ccacgccgat cgtctcatac gcgaggcgat ggaggcgggt
     1861 cggttcgatg atctccccgg tgtcggtgag cccattcccg gcgcaggcag caaagacgac
     1921 gccggctggt ggataagagg atggctcgag agaaatcgcg ccaatggtga tcagggtgcg
     1981 agcaactccg agtagagccg atggttggcc tcgtcgaagc agacgaagtt cacctcgtcg
     2041 acaccgtcgt tggcctcgac ccactctttg accgcggcga tggcagcggc cgcggcgtcg
     2101 gccttggggt acccgtaaac cccg
//
LOCUS       DASUTA010000300         9538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_474464, whole genome shotgun sequence.
ACCESSION   DASUTA010000300 DASUTA010000000
VERSION     DASUTA010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474464"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFZ80_05930"
     CDS             complement(<1..191)
                     /locus_tag="VFZ80_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287006.1"
                     /translation="MLRFTDPFDEMDRLMSSLGGRWRGGIMPVDAYEKDGIYTLRFDL
                     PGVEPDNVDLTVEGSVLTVT"
     gene            641..1021
                     /locus_tag="VFZ80_05935"
     CDS             641..1021
                     /locus_tag="VFZ80_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6287007.1"
                     /translation="MLLEREGELGLLTDLLADVGSSGGKVVLIRGEAGTGKSSLVGGF
                     LSSAADIAHTQFGFCDDLQTPQPLGPLWDISRSEPALRKALESRDRQAVLESFFDLLA
                     GSLRPTVVVIEDTQWSDEATRDVI"
     gene            1037..3202
                     /locus_tag="VFZ80_05940"
     CDS             1037..3202
                     /locus_tag="VFZ80_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6287008.1"
                     /translation="MASANGLLLLTYRIGEVDRDHPLRGVIGDLPPQNVVRIELGGLS
                     RSGVAELVSDSGLDPDLVFSATNGNPFLVTELARFGGEDVPASVRDSVVSRIGKLSST
                     ARDVLRVMSVIPDRISREDIPRLTGGSDDDLAEGERLGLLEVDGDLVSFRHELIRRAV
                     ESSLTISESVGIHRRLLDVLPDGTDPSRLVHHARGANDIDRLVVHAPVAALAAAEVGS
                     NREAAAHFRALEPHLSLFEPHRRADILSRWALIEHYLEMVESIEILDRAIELYREAGS
                     VEDLARALVLGVEVNRTHGRPDVARACALEAIRLLESGQPSHRLAHALAAYSWLLIHS
                     GQIDQAEAAADRAISVAEENGAELALISALGVKGTLVYVRGESGGLEIMERLQQRARL
                     GGYRYEEVLAQLRMAEIALEIRDIDRASDFSVRAKSTASRYELPILETKASMVRAEAL
                     LWAGAWSEAEDLATETIGRRSTADVRLAAILGLLRTRTGRGSAVEYLEQAWSLAVESG
                     EIDHLLRSASAVAERMWVVDTTDDALLEEFRDLVDRGIRFEYPWPAGSLALWLWKSGD
                     LDEPPEGLPAPYRDLLSGRVDQSASFWTSRQIPYEKAIALMSGEVSQRLEALELLNTL
                     GAVAVAARVSKELRDEGVPTPRGKGRATRGHAAGLTARQAEVLQSLSEGLTNTEIADR
                     LFVSPRTIEHHVSAILAKLDSSTRDEAVQRARDEALIET"
     gene            3309..3560
                     /locus_tag="VFZ80_05945"
     CDS             3309..3560
                     /locus_tag="VFZ80_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEX6287009.1"
                     /translation="MSLYMDVHTIAGGVSVADVADAHVKDLQTGPKYGVSYLRYWVDE
                     TAGKIFCLVEADNAEDANTVHREAHGLVADEIYPVSEHN"
     gene            complement(3659..3826)
                     /locus_tag="VFZ80_05950"
     CDS             complement(3659..3826)
                     /locus_tag="VFZ80_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287010.1"
                     /translation="MVEVGWAPLRATGFSGMDVVVFDVVAMVAQWTAAEVYSRACWLP
                     GVGRRLTGECD"
     gene            complement(4706..4780)
                     /locus_tag="VFZ80_05955"
     tRNA            complement(4706..4780)
                     /locus_tag="VFZ80_05955"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4743..4745),aa:Ile,seq:gat)
     gene            complement(5388..6557)
                     /locus_tag="VFZ80_05960"
     CDS             complement(5388..6557)
                     /locus_tag="VFZ80_05960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6287011.1"
                     /translation="MTRLVIVGGASMGWASAFHLTRMDPGLEVVVVERDPTLRYSSTM
                     LSDGNVRIQFNLAENIAISQYAMETLETFTREFSVGGFVPDPQMKKQGNLFLVDEAGR
                     EAALSGLATQQEMGCDAMWLDKTDVEARFPALRSDDLIGGTFGPSDGSVDPNAVVEGY
                     RRTALASGVATIAGEVDSITVGRDRVEGVSLADGSSLEADFVLVCAGAWSPQLLETVG
                     VDVPVEPVMRTVYVVAGDVIDGGSLPSVFLPSGVYVIPEHDGTYLIAWSTDDDPVGFD
                     FTPAPRSRFYDLIWPELAGSLPAFDRLEVVRSWAGLYAQNRLDANAIVGEWPHNPGLY
                     LATGFSGHGFQQCHAVGRYLAELITGTEPTLDLGRLGPRRILDNQPLLEHAGRII"
     gene            6656..6976
                     /locus_tag="VFZ80_05965"
     CDS             6656..6976
                     /locus_tag="VFZ80_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287012.1"
                     /translation="MARAESLIESRQYVLRSEWGEVQPRAEDENDFLEKHGWGEYSEW
                     HLGLTVGATEETKGRYAFVYGDFRRLHRMGLIACRYRAAEWGHKDIELAAHDLLQILD
                     SKRS"
     gene            complement(6977..8035)
                     /locus_tag="VFZ80_05970"
     CDS             complement(6977..8035)
                     /locus_tag="VFZ80_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEX6287013.1"
                     /translation="MTASRWMRATDILDGFALPAELEAARPPEKRGVARDGVRLMVTT
                     CEGVRHRRFTELVDVLSPGDLLVVNNSKTLPAAVALDDSRVVHFSTDLPGGLRVVELR
                     RSGIEGSHPDFDVDPGTTRLPDGSSLELLSPFPVDSTTRRLWVAHVDGGDGVDSLLRR
                     YGRPIGYSHTARDLPLEAYQTVFATTPGSAEMPSAGRPFSHRLVTGLVSRGVSIAPLT
                     LHTGVSSLESGEPPFPETYAVPQTTVDMVDLTRKRGGRVIAVGTTVVRALETITDPSG
                     RAHAGSGWTELVIGRDTALRAVDGMITGWHEPHSTHLDILVAVAGRRAVARSYETALE
                     HGYLWHEFGDSHLLINEG"
     gene            complement(8032..8718)
                     /locus_tag="VFZ80_05975"
     CDS             complement(8032..8718)
                     /locus_tag="VFZ80_05975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6287014.1"
                     /translation="MITGASRGLGRALADALARRDWDLVIDARTESDLTRAAGELDAL
                     GSGAVTSIAGDITDLHHVAALTSTAGETGRLDLLVNNASILGPSPQPTLDIYPIDVLR
                     DVIEVNLIGPLRLTQTSLPLLRETGGTVVNIPSDAAVEGYEGWGGYGSSKAALEQMSK
                     VLAAEEPALRVYWFDPGDMNTRMHQEAFPDEDISDRPNPEDRVPGLLTLIENRYPSGR
                     YTASELLVSA"
     gene            complement(8878..>9538)
                     /locus_tag="VFZ80_05980"
     CDS             complement(8878..>9538)
                     /locus_tag="VFZ80_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6287015.1"
                     /translation="GAIQIPGIDYREFLMAGIFAQTVVFGATFTGAGLAEDMQKGIID
                     RFRSLPMSRSAVLIGRTSSDVVFNVLSIAIMALTGLVVGWGIHTSVLEAAAGFGLLLL
                     FAYSFSWVMATVGLLVPSPEVVNNASFMVIMPLTFISNAFVPSDTLPTPLRIFAEWNP
                     VSAVTQAARNLFGNTSPLAPVPDVWSLQNAELYTLIWVVVLVAVFAPVSVHLYKRAAT
                     Q"
BASE COUNT         1829 a         2891 c         3075 g         1743 t
ORIGIN      
        1 gtgacagtga ggacactccc ctccaccgtc aagtcgacat tgtctggctc cacgccgggg
       61 agatcgaaac gaagtgtgta gatgccatcc ttctcatagg cgtcgacggg catgatccct
      121 ccacgccagc gccctccgag cgacgacatc agccggtcca tctcgtcgaa ggggtcggtg
      181 aaacgcagca tcgtgacctc ctaactcctt tcgtcatttc gatggcatcg atccagtcga
      241 gaacgtatgc cttcagatgg ccttacataa tctatgtata accgactcag attatgactg
      301 tcaagctccg ccatgatcgc agaggtcagt tgagcggtcc ggagatcgcg acaacacaac
      361 gagtatcgag ctcatcctca gctcgacctc atgtttcggc ctcgccggga agacgacatc
      421 ccagtcggtg ctcggtcacc agcggtggct cctctacctg ggcttcgccg ctctccctcg
      481 tctattgcac atcttgacac acgccggttt ccctccgacc gggggaacct catgccgagc
      541 ggaagctcac cggtccaacg gaacaggatc cgccctcgat tcgtcggcct ccgcgtgttc
      601 aacgccctcc ggatttcgca tgacatgtag cctcgggtcg gtgcttctgg agagagaggg
      661 agaactcggt cttctcactg accttctcgc cgacgtcggc tcatcgggcg gcaaggtcgt
      721 gttgatccgg ggtgaggccg ggaccggcaa gagctctctg gttgggggat ttctgagctc
      781 agctgcggac atcgcacaca ctcaattcgg tttctgcgac gacttgcaga caccgcagcc
      841 gttgggccct ctttgggaca tctcccgctc cgaaccggcg cttcggaaag cactggagag
      901 ccgtgaccgc caggcggtgt tggagagttt cttcgacctc ttggcagggt cgctgcgtcc
      961 caccgtggtg gtgatcgaag atacccagtg gtccgacgag gcgacccggg acgtcatcta
     1021 gtatgggggc cgtcggatgg ccagcgccaa tgggctgctt cttctcacct atcgcatcgg
     1081 agaagtcgac cgtgatcacc cgctgcgggg ggtgataggg gatttgccac cgcaaaacgt
     1141 tgtccgtatc gagctcgggg gtctttccag atccggcgtg gctgagctcg tcagcgactc
     1201 agggctcgat ccggacctcg tgttctcggc gaccaacggc aacccgtttc tggtgactga
     1261 gctggcccgg tttgggggtg aggacgtgcc cgcctcggtg cgggactctg tggtttctcg
     1321 gatcgggaaa ctgtcctcga ccgctcgtga cgtgttgaga gtcatgtcgg tgatcccgga
     1381 ccggatctcc cgggaggaca ttccccggct caccggtgga tcagatgatg acctcgccga
     1441 aggtgagagg ctggggctgc tcgaggtaga cggcgatttg gtctcgtttc gtcatgagtt
     1501 gatcaggcgc gccgtcgagt cgtctctgac gatcagtgaa agcgtgggca tccaccgtcg
     1561 tctcctcgac gtgctccccg atgggacgga tccgtccagg ctggtgcatc acgcccgcgg
     1621 agccaatgac atcgaccgcc tcgtggtcca cgcccctgtg gcagctctgg cggcggcgga
     1681 ggttggaagc aatcgtgagg cggcagccca tttccgtgcc ctcgagccgc acctgagcct
     1741 gttcgagccg cacagacgcg ccgacattct cagccggtgg gctctgatcg agcactatct
     1801 ggagatggtc gaatccatcg agatcctcga ccgggcgata gagctgtatc gggaggccgg
     1861 ctccgtcgag gacctggccc gggcactggt attgggtgtc gaggtgaacc ggacccacgg
     1921 gcggcccgat gttgccagag catgtgccct cgaggcgatc cggctcctgg aatccgggca
     1981 gccgagtcat cgtctggccc atgccttggc tgcgtactcc tggctgctga tccactcagg
     2041 tcagatagac caggccgagg ctgccgccga ccgcgcgatc agtgtcgccg aggagaacgg
     2101 agccgagttg gccctgatca gtgccctcgg cgtgaaggga actctggtgt atgtgcgagg
     2161 ggagtccggc gggctggaga tcatggagcg attgcagcag cgcgcccgtc tgggcggcta
     2221 ccgctacgag gaagtgctgg cccaactccg gatggcggag atcgccctgg agataaggga
     2281 catcgacagg gcgtccgact tctcggtgcg ggcgaagagc accgccagcc gctacgaact
     2341 acccatcttg gagacgaaag cgagcatggt gcgcgccgag gccctgctct gggcgggcgc
     2401 atggtcagag gccgaagacc tcgcgacgga gacgatcggc cgacgctcca cagccgacgt
     2461 gcggctcgca gccatactcg gtctgttacg aacccggacg ggtcgtggca gcgccgtcga
     2521 atacctggaa caggcatggt ccctggcagt cgagagtggt gaaatcgacc acttactgcg
     2581 ttctgcttcg gccgtcgccg agcggatgtg ggtcgtcgat acaaccgacg acgccttgtt
     2641 agaagagttc cgggacctcg ttgaccgcgg aatcaggttc gaatatccct ggccggcggg
     2701 atctttggcc ctgtggctgt ggaagtcggg tgatctcgac gagccgccgg aggggcttcc
     2761 cgcaccgtac cgggacctcc tgtcgggccg ggtcgatcag tcggcgtcgt tttggacgtc
     2821 gagacagatc ccgtatgaga aggcgatcgc cctcatgtca ggagaggtgt cacagcgtct
     2881 agaggcgctg gaactgctga acactctcgg cgcagtggcc gtggcagccc gggtgagcaa
     2941 ggaactgcga gacgagggtg ttccaactcc gcgcggcaaa ggccgagcga ctaggggcca
     3001 cgccgcgggt ctcaccgccc gccaggcaga ggtcctccaa tctctctccg agggcttgac
     3061 taacacagag atcgcggacc gcttgttcgt ctctccgagg accatcgagc accacgtttc
     3121 ggcgattctg gcgaaactcg actcctccac ccgagacgag gcggtgcaaa gggcccgaga
     3181 cgaagcgctc atagagacct aaagcgcgta cctccccacc catatctggg ggtatctccc
     3241 cgatggcccg taccccggtc gtagatacgg tcatggagag atatcgacct agaactcagg
     3301 aggttcatat gtcgctgtac atggatgttc acaccatcgc gggaggcgtc tccgtcgctg
     3361 atgttgcgga cgcgcatgtc aaggacctac agaccgggcc caaatacggg gtgagctacc
     3421 tccgctattg ggtcgacgag acggcaggaa agatcttctg tctcgtggag gccgacaacg
     3481 cagaggacgc caacaccgtt caccgggagg ctcatgggct cgtcgccgac gagatctacc
     3541 cggtcagtga acacaactga ccggaccgaa agaaagtcaa cagagaaatc aggagagaca
     3601 aatgaagaga atcatcacag tggggatgct gacggcaacg atggtgctgg ccactgcatc
     3661 agtcgcattc gccggtgagc cgccggccaa cgccgggcaa ccaacatgcg cgggagtaga
     3721 cctcggcagc ggtccactgg gcaaccatgg cgaccacatc aaagacgact acgtccatcc
     3781 cggagaaccc ggtggcgcga aggggggccc agcccacttc aaccatccac ccgaggtagg
     3841 tccaggcgca tcgttctgcc tcgagcaggc gcaggctgag tcgccgtcga caccccgagg
     3901 acaggcataa acacaaagca ccccatacga ggaccaggaa aggaagcacg atgctccgtt
     3961 acagatccgc ggttgccctc caccgtctga ataacaccgt ggcgaatccg tggaatgtgt
     4021 cgagcggact gatccgaagg atgagcgaag gcaagatgag accgaaccga tcctcgaact
     4081 tcgcgacaga gccgagatcc aggaggcaaa agatgagatt cgtcaagcgc taccgccgtg
     4141 tcaccactgc cagggcattg aagccatgga cccccttatt cacgctcgac ggccacgagg
     4201 tcaccaccac attcgccaaa ggccggttgt gaggatcccg ttctccctcc gttccatccc
     4261 ggcgctggcc ttcgggcctg gctgacaccg ccgactctgg cctccatcgc cgcacagctc
     4321 gacatcgctc cacgtcgatc ttgcggggga aggttctgga gcgctgacgc ccgtcgggcc
     4381 gcgggagggg ccaggcctgg tccggtgggg cttacgctct gatgacgatc ccatccagtt
     4441 tccctgtctg ttgcaccgat gttgcacatg actctgaaac agtgaagttc gccgacctgg
     4501 gatgatccct agaaataggg cgtgactagg gaaaatgcac cgtgatccgc aactcactgg
     4561 gtctcacgac gatggacctc atccttttcg ggaatgggcg ctagaacatt gtccaggtcg
     4621 caacggatcc gacaacgggg ctatgagttc ggggacctcc acctggtcaa aagcagtttc
     4681 tgagtctttc caggtgaatg aattgtgggc ccacgtggac ttgaaccacg gacctcatcc
     4741 ttatcaggga tgcgctctaa ccgggctgag ctatgggccc cgactggtag tcaaggatct
     4801 cgaagtgtag aagcgacccc ggccttcatg aagtcggttc gcaggtgaga agggactcct
     4861 gacacgggca cctttgtggt tggagggatg cccacgccga gtgggccgtt tccgcatggg
     4921 cgtccacggg taggcctcgg cggcagtaac ggatcgttgt gagttcaaat ggagatctcg
     4981 acaagcagcc aggcaacggg cgggtttgat cgtcgcgacc gtcctcttcg tggagtaggg
     5041 cggctacgtg gtcttgcgga tgagcctggg ccagaagccg gccatccgtt ccaggtcacg
     5101 tttgccaggg ccggcgatgg tcacccggct gtgctcctgg tgttggcact ggtaggcccg
     5161 atcctcgccg atttcaccga cctctcaggc gtttggcgag tcatcgcgcg cagtggcatc
     5221 tgggctgccg ccatcctctt tccggcaggt ttcttcctcg cctcggccgg cccgggcagg
     5281 gaaaccgcca gtcggtggat ggcactcgtc tatgtcggtg ccggagtcct ggcgctggct
     5341 gcggccaccc tcggtgtggg tctccttctg gcggccggtt tatcgcgtca gatgatgcgc
     5401 ccggcgtgct ccaggagcgg ctggttgtcg aggatccgtc gagggccgag tctgcccaga
     5461 tcgagggtcg gctcggtccc ggtgatcagt tctgccagat atctgccgac agcgtgacac
     5521 tgttggaacc catggccgga gaaacctgtt gccaggtaca gaccaggatt gtggggccac
     5581 tcgccgacga tggcgttggc gtcgagtcgg ttctgcgcgt agagaccggc ccaggagcgg
     5641 accacctcga gtcggtcgaa tgctggcagg gagccggcca gctcgggcca gatcaggtcg
     5701 tagaacctcg accttggcgc cggtgtgaag tcaaaaccaa ccgggtcgtc gtcggtggac
     5761 catgcaatca ggtaggtccc gtcatgctcg ggaatcacgt acacgccgga gggcaggaac
     5821 acactcggga gggagcctcc gtcgatgacg tcgccggcaa cgacatagac ggtgcgcatg
     5881 acaggctcca ccgggacatc gaccccgacg gtctcgagga gttgggggga ccaggctccg
     5941 gcgcaaacca gcacgaagtc ggcttcgagg ctggagccat cggcgaggga tacgccttcg
     6001 actcgatcgc gaccgacggt gatcgagtcg acttcgccgg caatggtggc gactcctgac
     6061 gccagggcgg tccgtctgta cccctccacc accgcatttg gatcgaccga gccgtcgctg
     6121 ggcccgaagg tgcctccgat caggtcgtct gatcgcaggg cggggaacct cgcctcgaca
     6181 tctgtcttgt ccagccacat cgcgtcacac cccatctcct gctgcgtcgc cagaccggat
     6241 agggccgcct ctctgcccgc ttcgtcgacc aagaagagat tgccttgctt cttcatctgc
     6301 gggtcgggaa cgaagcctcc gaccgagaat tcccgggtga aggtttcgag cgtctccatg
     6361 gcgtattggg agatggcgat gttctctgcc agattgaact ggatccggac attgccatcg
     6421 gagagcatgg tcgaggaata gcggagggtg ggatctcttt cgaccacgac gacttcgaga
     6481 cccgggtcca ttcgggtcag atggaatgca gaagcccagc ccatcgaggc tccgccgacg
     6541 atgacgagtc gggtcatggg ccgccagggt aaatgagcgt ttcgcctccc gatgccccgg
     6601 gtaggccttt cggccagagg aggagaacat cgagtacgaa gtcaatcccg ccggtgtggc
     6661 ccgagcagag agcctcatcg agtcccgtca atacgtgctc cgcagtgagt ggggcgaggt
     6721 gcaaccccgc gccgaggatg agaacgactt cttggagaaa cacggttggg gcgagtactc
     6781 agagtggcat ctcggtctca ccgtgggagc aaccgaggag acaaagggcc gatatgcatt
     6841 tgtgtacggg gacttcaggc ggctccaccg gatggggttg attgcatgca gataccgggc
     6901 agccgagtgg ggtcacaagg acatcgagct cgccgcccac gatctcttgc agatcctcga
     6961 ttcgaagaga agctgatcaa ccttcattga tcaggaggtg gctgtcaccg aactcgtgcc
     7021 acagatagcc atgctccagc gcggtctcat agcttcgtgc caccgctcga cgcccggcca
     7081 ccgccaccag gatgtcgaga tgggtcgagt gcggctcatg ccagccggtg atcatcccat
     7141 cgaccgcccg caaggccgta tcccggccga tgaccagctc ggtccagccc gaaccggcgt
     7201 gggctcgacc cgaagggtcg gtgatggtct cgagtgctcg gacgactgtc gtgccgactg
     7261 cgatcaccct tcctccgcgt tttcgtgtca ggtcgaccat gtcgacggtg gtctgtggga
     7321 ccgcgtatgt ctccggaaat gggggctcgc ccgattcgag agatgagacc ccggtgtgga
     7381 gggtcagcgg tgcgatcgag acgcccctgc tgaccagacc cgtgacgagc cggtgactga
     7441 aaggccggcc agcgctgggc atctcggcgg acccaggcgt tgtggcgaag accgtctggt
     7501 acgcctcgag gggaaggtca cgtgcggtgt gtgagtatcc gatcggccga ccgtatcgac
     7561 ggagcagcga gtccactccg tcaccgccat cgacatgtgc cacccaaaga cgtctcgtcg
     7621 tggaatcgac cgggaacggc gagagcaact ccaaactcga cccgtcgggc agccgggtcg
     7681 tccccggatc gacgtcaaaa tcggggtgcg agccctcgat tcccgacctt cgcagctcga
     7741 ctacccgaag ccccccgggt agatccgtgg agaagtggac gacgcgtgag tcgtcgagag
     7801 ccacggcggc ggggagggtc ttcgaattgt tcaccaccag gagatcgccc ggagacagca
     7861 catcgacgag ttcggtgaac cggcgatgcc ggactccttc gcaggtcgtc accatgagtc
     7921 gcaccccgtc ccgagctacg cctcgcttct caggcggtct ggccgcttcc agctccgcag
     7981 ggagcgcgaa tccgtcgagg atgtcggtgg cccgcatcca gcgagatgcg gtcacgccga
     8041 caccaggagc tccgaagcgg tgtaacgccc gctcggatac ctgttttcga tgagagtgag
     8101 gagaccgggg acacggtcct ccggattcgg ccggtcggag atgtcctcgt cgggaaaggc
     8161 ctcctgatgc atgcgggtgt tcatgtcacc cggatcgaac cagtagaccc tcaaagcagg
     8221 ctcctcggcg gcgaggacct tcgacatctg ctcgagagct gctttcgatg aaccgtatcc
     8281 gccccagcct tcatatccct cgaccgcggc atccgagggg atattgacga cggtgccgcc
     8341 tgtctcgcgc agcaagggga gggaggtctg agtgaggcga aggggaccga tcaggttgac
     8401 ctcgatcaca tcgcgaagga catcgatggg gtagatgtcg agagtgggct gcgggcttgg
     8461 cccgaggatg gaggcgttgt tgaccagtag gtctaaccga cccgtctccc cggctgtcga
     8521 ggtcagggcg gccacatggt gcaggtcggt gatgtccccg gcgatcgagg tcaccgcgcc
     8581 agatccaaga gcatccaact caccggctgc ccgggtcaga tcggactcgg tcctggcatc
     8641 gatcaccaga tcccaatctc gtcgggccaa cgcgtccgcc aaagctctac cgaggcctcg
     8701 ggaggctccg gtgatcaacg cagtgctgat aggtactcca aacgtccagt tccgtcagag
     8761 tacgacttga agttaacttg aagtcaaggg gatttgtccc caaaggacct tgcgaggcgt
     8821 tgctatagca agatgtcgtg gaatccgagt cctgatgcta ttgcaacgca cgcggaccta
     8881 ctgggtggcg gctcgcttgt agagatggac ggagaccggg gcgaagactg cgaccagcac
     8941 gacgacccag atgagggtgt acagctcggc gttctgcagc gaccacacgt cgggcaccgg
     9001 ggccagtggg ctggtgttgc cgaacaggtt tctcgccgcc tgtgtgaccg ccgacaccgg
     9061 gttccattcg gcgaagatcc gcagtggagt gggcagcgtg tcgctgggga cgaaggcgtt
     9121 cgagatgaac gtcagcggca tgatcaccat gaacgaggcg ttgttgacca cctccgggct
     9181 ggggaccagc aaaccgaccg tcgccatcac ccatgagaac gagtaggcga acaacaggag
     9241 caacccgaat ccggctgcgg cctcgaggac cgaagtgtgg ataccccatc cgaccaccag
     9301 tcctgtgagg gccatgatgg cgatcgagag cacgttgaag accacatcgc tgctggtgcg
     9361 accgatcagg accgccgagc gcgacatcgg aagcgatcgg aatcgatcga tgatgccctt
     9421 ctgcatgtcc tctgccaggc cggctccggt gaacgtggct ccgaacacga ccgtctgggc
     9481 gaagatcccg gccatgagga attcgcggta gtcgatccca gggatctgga tcgcgccc
//
LOCUS       DASUTA010000301         3204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_605770, whole genome shotgun sequence.
ACCESSION   DASUTA010000301 DASUTA010000000
VERSION     DASUTA010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605770"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /gene="pheS"
                     /locus_tag="VFZ80_05985"
     CDS             <1..233
                     /gene="pheS"
                     /locus_tag="VFZ80_05985"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010288230.1"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 1 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX6287016.1"
                     /translation="CDGGEPGCRVCGGKGWLELLGCGMVDPYVLDAAGYDSSQLSGFA
                     FGMGPERLAMTRHGIDSLRHFIENDIRFLEQF"
     gene            286..2676
                     /gene="pheT"
                     /locus_tag="VFZ80_05990"
     CDS             286..2676
                     /gene="pheT"
                     /locus_tag="VFZ80_05990"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX6287017.1"
                     /translation="MKVSLSWLQEFIDLPTTDVNELSYAFDMLGHTTEGIEYLEIDWS
                     DVYVGEVLEIDSHPDADKVRVCQVDSGEGPTQIICGAWNFESGAMVAVARPGAVLPGD
                     FEIGQRSIRGVESNGMICSERELGLGDDHAGILVLDGDPKIGSPFAELVELPDVVFDL
                     DVPTSRPDALSLVGIARDLAAWFEVDFRVTPVDMPTVPGATAISVEIADPTGCRRFTA
                     REIRGVTVGRAPLKMRHRLHKVGTRSVSDVVDVTNYVMYELGHPLHAFDADSIVGDRL
                     VVKRAEPGEKLETLDHVNRALSTDDLIIYDEAGPTSMSGTMGGARSEVSESTKRVLME
                     AASWDPPTIMHMWRRHDLRSEAATRFERGVDPNLADIANQRASAMVQAISGGEILEGA
                     VDEIAVHVEPWTVELPAGECERLLGPGFEPPYVTSILERLGMSVDGDDPLIVTVPTYR
                     PDLTRPADLVEEVARIHGFDKFDATLPTGPAGGLSESQRRQRTLRATLTGAGLTQAIN
                     LPFVSVAELVGLGHETDGSDLLTIKNPLRDEESKLRPTLLPGLLNDLRYNQSHGNASI
                     GLFEVGKVFFSDPDPTDPRLPHEYDRLAWAVVGDVGARGLDGSPFRADAAFSLGVWRL
                     LSEALGLDATVEQATRPGFHPGRTAQISIGDEVIGHVGELAPSTARFFGVEGRVAVAE
                     LNLEPLLREVPHRLFSTPSVFPHVDFDLSFLVSSSTPASDLLAATTDAAPGLVESARV
                     FDEFRSESVGEGQKALAITYRLRASDRTLEQKEIGSIRQNMIEAAAEVGAKLRGAE"
     gene            2673..>3204
                     /locus_tag="VFZ80_05995"
     CDS             2673..>3204
                     /locus_tag="VFZ80_05995"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX6287018.1"
                     /translation="MTKTGALASILTSPPEEAAPRLLGSLLVSTVDGHEVVARINEVE
                     AYKGSDDPASHAFGGETRRNTSMFQEPGTLYVYRSYGIHNCANSAAGPKGVGWGILIR
                     GGDVVEGSGVAARRRGRKTELANGPGKLCQALGITLDLNGTDLLDSTSIVHLEPGEPP
                     DYVMATPRIGISRARDL"
BASE COUNT          585 a         1001 c         1031 g          587 t
ORIGIN      
        1 catgtgatgg cggtgagccg ggatgcagag tctgtggggg gaaagggtgg ctcgagctcc
       61 tcggctgtgg aatggtcgat ccctacgtgc tcgacgccgc cggctatgac tccagtcagc
      121 tgagcgggtt cgcattcggc atgggcccgg agcgtctggc gatgacacgc cacggcatcg
      181 attcactccg tcacttcatc gagaacgaca tccgcttcct ggagcagttc tgatgcgtgg
      241 cgcttctcgc gcggcggtag caccgaccga ccgggagcag ttctcatgaa ggtttcactg
      301 agttggcttc aggagttcat cgatctgccg accacagacg tgaacgagct ctcatatgcc
      361 ttcgacatgc tcgggcacac cacggagggc atcgagtacc tcgaaatcga ttggagcgac
      421 gtctacgtgg gtgaggtgct ggagatcgac tcacatcccg acgccgacaa ggtcagagtc
      481 tgtcaggtcg acagcgggga gggccccact cagatcatct gtggtgcctg gaacttcgag
      541 tcaggagcga tggttgcggt cgcccggccg ggcgccgtct tgcctggcga cttcgagatc
      601 ggccagcgct ccataagagg agtcgagtca aatgggatga tctgctcaga gcgggagctg
      661 gggctgggcg acgatcatgc cggaatcctc gtcctcgatg gagatcccaa gatcggctcg
      721 cccttcgccg agctcgtcga gctgcccgat gtcgtgttcg atctcgacgt tcccaccagc
      781 cgtcccgacg ccctctctct ggtcgggatc gcccgtgacc tggcagcctg gttcgaggtc
      841 gattttcggg ttactcccgt ggatatgccc accgtccctg gggcgacggc catatcggtc
      901 gaaatcgccg atcccaccgg ttgtcgtcgc ttcacggcgc gggagatcag gggggtgacc
      961 gtcggccggg caccactgaa gatgcgccac cggctgcaca aggtgggcac cagatcggtc
     1021 tccgatgtcg tggacgtgac caactacgtg atgtacgagc tcggtcaccc ccttcatgcc
     1081 ttcgacgccg actccatcgt cggcgacaga ctcgtggtca agagagccga gccgggcgag
     1141 aagctggaga ccctcgatca tgtcaaccgg gcattgtcaa cggacgacct catcatctat
     1201 gacgaagcgg ggccgacctc gatgtcggga accatgggtg gtgcccgcag cgaagtgtcg
     1261 gagagcacca agcgggttct gatggaggcc gcctcatggg atccgccaac catcatgcac
     1321 atgtggcgtc gtcacgatct ccgatccgag gcggcgaccc gcttcgagcg cggagtcgac
     1381 cccaacctgg ccgacatcgc caaccagcga gccagcgcca tggtccaggc gattagtggt
     1441 ggcgagatcc tcgaaggtgc tgtcgatgag atagccgtac atgtagagcc ctggacagtc
     1501 gagctcccgg cgggcgaatg cgagaggctc ctcggacccg ggttcgagcc tccatacgtg
     1561 acatcgatcc tggagcgctt gggcatgtct gtcgacggtg acgacccgct gatcgtgacc
     1621 gttcccacct atcgccccga cttgacgcgg cccgccgacc tggtggagga agtcgcccgt
     1681 atccacggct tcgacaagtt cgatgccacc ctccccaccg ggcccgccgg cggcctcagt
     1741 gagtcgcagc gacggcaacg cacgttgcgc gccaccctca ccggtgcggg tctcactcaa
     1801 gccatcaacc tgccgttcgt cagcgtggcc gagctggtgg gattgggaca cgagacagac
     1861 ggctcggacc tcctcacgat caagaaccca ttgcgggacg aggagagcaa gttgaggccg
     1921 acgctcctgc ctgggctcct caacgatctc cgctacaacc aatcgcacgg caacgcttcc
     1981 atcggtctct tcgaagttgg caaggtcttc ttctcagacc cagacccgac cgatccccgg
     2041 ctgcctcacg agtacgatcg tctcgcctgg gccgtggtgg gcgacgtcgg agccagaggg
     2101 ctcgatggat cacctttcag ggccgatgcg gccttctcgt tgggagtctg gcgactcctg
     2161 tccgaagcac tcggcctgga tgccacggtc gaacaggcga cacgtccggg tttccacccg
     2221 ggacgcactg cacagatctc catcggtgac gaggtcatcg gccacgtcgg cgagctggcg
     2281 ccatcgacag cccgcttctt cggggtcgaa gggcgagtgg ctgttgccga gttgaatctc
     2341 gagccgttgc tgagagaggt tccacaccgt cttttctcca cgccgtccgt cttcccacat
     2401 gtggacttcg acttgagctt cctggtgtca tcctcgactc cggcgagcga cctcctggcc
     2461 gctaccacgg atgcggctcc aggactcgtg gagtccgctc gggtcttcga tgagttccgg
     2521 agcgagtccg tcggagaagg acagaaagcg ctggcgatca cctatcggct aagggcatcc
     2581 gatcgcacgc tcgaacagaa ggagatcggc tcgattcgtc aaaacatgat cgaagccgcg
     2641 gccgaggtgg gtgcgaaact cagaggcgcc gagtgaccaa gaccggcgcg ctggcatcga
     2701 tcctgacgag tccgcccgag gaggccgcac cacgtctgct cggttcactt ctggtttcca
     2761 ccgtggatgg tcacgaggtc gtggcacgaa tcaacgaagt cgaggcttac aagggctcgg
     2821 acgatcccgc ctcgcacgct ttcggcgggg agacgaggcg caacacttcg atgttccaag
     2881 agccgggaac gctgtacgtc taccggtcct atgggatcca caactgtgcc aactcggcgg
     2941 ccgggccgaa gggagtcggg tgggggattc tgatcagggg aggcgatgta gtcgaaggct
     3001 caggcgtcgc cgccaggcgc cgggggagga agaccgagct cgccaatggg ccaggaaagc
     3061 tgtgccaggc cctgggtatc accctcgacc tgaatgggac cgacttgttg gactcgactt
     3121 cgatcgtgca tctcgagccc ggggagccgc ctgactacgt gatggccacg ccgcgcatcg
     3181 gaatctcgag ggcacgtgat ctgc
//
LOCUS       DASUTA010000302         2397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_218437, whole genome shotgun sequence.
ACCESSION   DASUTA010000302 DASUTA010000000
VERSION     DASUTA010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218437"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFZ80_06000"
     CDS             complement(<1..197)
                     /locus_tag="VFZ80_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEX6287019.1"
                     /translation="MSQVFLANLGADARHVPICLLLSGAMTTGSPIWDGIIIGVAVTV
                     LVGFAGYLLRNRRRVRNERLP"
     gene            216..440
                     /locus_tag="VFZ80_06005"
     CDS             216..440
                     /locus_tag="VFZ80_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287020.1"
                     /translation="MGRLERDRAYRITARRGGTELTVIGIFDHEYRAYDPPILVFRHT
                     EAPMPAFGFPQGFLVRSDEVVSIVPLSNGE"
     gene            complement(491..564)
                     /locus_tag="VFZ80_06010"
     tRNA            complement(491..564)
                     /locus_tag="VFZ80_06010"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(528..530),aa:Ala,seq:ggc)
     gene            complement(1020..1370)
                     /gene="rsfS"
                     /locus_tag="VFZ80_06015"
     CDS             complement(1020..1370)
                     /gene="rsfS"
                     /locus_tag="VFZ80_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX6287021.1"
                     /translation="MARAAADALFAKNGADIVLLDVEEAFFLSDVFVIATGSSRINVQ
                     ALADHVEERLGELYGVKPLRVEGRTEGEWCLVDFGDIIVHIFQGEPREYYSLERLWGD
                     AGRIMWEEPVVTDG"
     gene            complement(1412..1993)
                     /gene="nadD"
                     /locus_tag="VFZ80_06020"
     CDS             complement(1412..1993)
                     /gene="nadD"
                     /locus_tag="VFZ80_06020"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX6287022.1"
                     /translation="MRIGILGGTFDPIHIAHLHAAECARHQLGLDRVLVMPAGDPWQK
                     AQRRVTSDEHRVAMCRLAVEGAEGIEVDEREIQRVGPTYTVDTLATFSDEDQLFLILG
                     GDAASRLHTWHRWKEVVNRATIVIVPRPGAPVGDVSGSVQIDMGALEVSGTEIRQRAA
                     DGRPFRYLVTRQVYDYIASHGLYAKEQRDDMVG"
BASE COUNT          503 a          762 c          705 g          427 t
ORIGIN      
        1 aacggaagcc gctcattccg tacgcggcgg cggttgcgaa gtaagtagcc agcaaagccg
       61 accagcaccg taacggcgac cccgataatg atcccgtccc agataggaga tccggtcgtc
      121 atggctccgg agaggagaag gcagatagga acatgacgtg cgtcggctcc caggttggcc
      181 agaaaaactt gactcacgcg cttagggtca ccggcttggg cagactcgag cgcgacaggg
      241 cctaccgaat cacagcgaga cgcggcggca ccgaactaac cgtgatcggc atattcgatc
      301 acgagtaccg ggcttacgat ccgccgattc tcgtgttcag gcataccgaa gcgccaatgc
      361 ccgcctttgg gtttccacaa gggtttttgg tgcgatcaga cgaagtagtc agcatcgtgc
      421 ccttgagcaa cggggagtga taggcaccca cctgggcacc caagcgacgg ggaagaccaa
      481 gaaaagccag tggagctgag gggatttgaa cccctgaccc cttgactgcc agtcaagtgc
      541 tctgccgaac tgagctacag ccccagacgc cctgtgcagg tttacatgac aacgcggccg
      601 tatctgcaca cgctaggagc ggccagtgta ttgccgggca gactcgaagg tgaaaccaac
      661 caagggtgcg cgatcggcga agtttggccg aggtagagaa ggggtacaca cggtcggagg
      721 tatcgacatg agactcctac caaccgcaca ccgagagcaa caacccctcc atcggagcag
      781 cagactgtgg gggttggcca ccagcctccg tgagcttgca caagagatga gggccgatgg
      841 cctgagggat gaagccgaca gtctcgaaac cacgattcgc gatctcgact ggcagtacga
      901 agtcttgatc gacagagaac tacttgcgga gtggcccttc ggtccgcggc gtgcttcacg
      961 cacaccactc cgagccgtcc gctgaccacc acccctcgtt gccatcgaac tacggtgact
     1021 caaccgtcgg tgacgacggg ctcctcccac atgattctcc cggcgtcacc ccagagtcgc
     1081 tccagtgagt agtactcgcg gggctcgcct tggaatatgt gaacgatgat gtccccgaag
     1141 tcgacgagac accactcccc ctccgtccgt ccctccaccc gcaatggctt gacaccgtag
     1201 agctcgccaa gtctctcctc cacgtggtcg gccagagcct ggacgttgat ccgtgacgaa
     1261 ccggtggcga taacgaagac atcggagagg aagaacgctt cttccacgtc gagcaagacg
     1321 atgtcggcac cgttcttggc gaacagggcg tcggcggccg cacgggccaa ttcgacactg
     1381 tgctctatga cgtggtctcc agcttcatga gtcagcccac catatcgtct cgttgttctt
     1441 tcgcatagag accgtgcgac gctatgtagt catacacctg gcgggtcacc aagtagcgga
     1501 atgggcgtcc gtctgcggcg cgttgcctga tctctgtgcc gctcacctcg agagccccca
     1561 tgtcgatctg caccgagcct gaaacatcgc cgaccggagc tccaggtctg ggcacgatca
     1621 cgatcgtggc ccggttcacg acttccttcc agcggtgcca ggtgtgaaga cgagaggcgg
     1681 catcgccacc gagaatcaag aagagctggt cctcatcgct gaatgtggcc aaagtgtcga
     1741 cggtgtaggt gggcccaacg cgttgaatct cgcgttcgtc gacctcgatg ccctccgccc
     1801 cctccactgc gagacgacac atcgccacgc gatgctcatc tgaagtaacg cgacgctggg
     1861 ctttttgcca cggatcgccc gccggcatca ccaggacccg gtccaagccc agttggtgtc
     1921 gagcgcactc cgctgcgtga aggtgggcga tgtggatagg gtcgaaggtt ccaccgagga
     1981 ttccaatgcg cacggcgacg agcataggga cgcggtggcg tgatcagccg aaggcccctc
     2041 gagatatccg ttttggcaca gccgagaagg gtcgtcggat cgacatactt cccgtcgatc
     2101 ctcgttgaca tgtgaccacc ggggacgccg tgtggtgttc cggccctccc aacttgtgca
     2161 ccccgccgga cctgttctcc gcgcgacacg aggatcgtcg ccagatagga caccgacacc
     2221 ttgtagccag gtacaggctc gatcgtcaca gttctcatcc ctgccaccga cccggcgaac
     2281 gtcactgtgc ccgacaccgg cgctctcacg acctcaccga ccgcggttgc gtagtcgacg
     2341 ccccaatggc ccgcatactg tcccgatggt gcgtacccaa cggtcaccgg cccgtcg
//
LOCUS       DASUTA010000303         4607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1512708, whole genome shotgun sequence.
ACCESSION   DASUTA010000303 DASUTA010000000
VERSION     DASUTA010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512708"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..4056
                     /locus_tag="VFZ80_06025"
     CDS             214..4056
                     /locus_tag="VFZ80_06025"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX6287023.1"
                     /translation="MELLRVHLFGGFLLERGGIALPPIASRAGRSLFAYLVMHRDRPL
                     NRELVAGTFWPELSETRARRRLSHTLWQIQDVVNTSSSSYLDAATDTLAFDANSAYWL
                     DVEEFDRQYAVAQGSPDDTRIATSYRASVLRTCVELYRGDFLAGFFDDWVVAEQDHYR
                     QRYMTALSRLIEATKSAGAYEEALGYARRMTHHDPLSEEAHREVMRLCFLLGRTGDAV
                     EQYRRCRSVLEDDLGADPSPATVELYEKIVRQRRAGIRVGFEESKPALSMRSDSPFVG
                     REDERRLLIDAVERVLAGPGGVALIEGEPGVGKTRLANEVVDDAKWRGFEVSWGSCRP
                     GALRPFAPVVEILESLTPIRLEQLAEQVAPIWFNQALRLAPQLAHRLDGSEEPAPLRP
                     AEELTRTQEALVHTLGALGRIAPHLLVIDDVQWADRDTLSVLTQLASRLAGTRVLVLA
                     VFRSEEARGEPEVWDVIRDLDRVAGLGRAVLSPLSVFELDEMVRRILEVPRLDPSVAT
                     RLHRETGGNALFALETLLAMRDRGRLEGGNDLLAGLEPPITGKELPVAPRVRAVIESR
                     ISLLGDEVAEVYQVASVIGDPVRLELLEAAAELARPVILDAVDELLHRGLLREVGNSV
                     YRLAHDQIRQVAYEAIASDRRERTHLAIAESLIRLDADQVEEIGHHFLMGGDYDRSAR
                     YLLRAGLRAVELSAFATAASHLQEARLAAIDAGWSDADRYALLGHLEDVLGVLGRRQE
                     SSAVIDEMEDLVDAVTEPELARRRSWLLAHEGRLSDAEEMAARSVALEEEAVSADLAD
                     SLVALGTIRRWSGRPLEAVSVLEDAVDAAGDNERLRARALTELASTLFALQRHADATP
                     LLEQAASIHERLIDLRGQAEVGGIRARALGEQGRRDEAQEQYSNTIELCRRIGYRHGE
                     GMNLVNLANLHQRHGNALAALEGYDRAAVIFKELNNPRGEAVVLANAASARHSILGDD
                     QRAKEDAQRAMGLFEGIGDLGHMAQCEEILAGITARSGDLDKALSMIEHSLRRLEGVG
                     DVALEVQHLRSLALVELELGDYASAMAAVDRAEQLAADVAVGDIAVDLWSVRGIIAIA
                     RGNTFEGLTEIRHAVERLGPGVERQYLIHHRHARAAHAAGEREEARLAAMRASTELEN
                     VIAGLDAEARRQAIEMVPEHRSIDEWARFYSPSTIDVALPATVAPMGRRLEPEEMVEL
                     RWTIEHPDDRSIEEGIDRRRHRLMRLLDEAGAYAASPSVEDLARALGVSASTVRRDIA
                     QLRRSGHDVRTRGQRNVS"
BASE COUNT          808 a         1416 c         1526 g          857 t
ORIGIN      
        1 ttgggtcatg cccgaaaatc ggcattttcg gcagtggctt gagggaccag gggtcaggta
       61 gcggtgtctg caaatcctgg gttggagact tcgctctgcg aagtccgcgg gcccggagaa
      121 cgagcggcga gtaggagcgc gtcaatcgcg gtcatcgact cagtcgggga ttggggacat
      181 tccgacccga cccatccggt taccgtttgg tgaatggagt tgcttcgtgt ccatctattc
      241 ggcgggtttc tcctcgagcg cggaggcatt gcactgcccc caatcgcctc cagggctgga
      301 cgttcactgt tcgcctatct ggtgatgcac agagaccgtc ccctcaatcg cgagttggtg
      361 gcgggcacct tctggccgga gctttccgag acgagggctc ggcgacgttt gagccacacg
      421 ctgtggcaga tacaagacgt tgtaaacacg tcttcttctt cctatctcga cgctgccacc
      481 gacaccctcg ccttcgacgc caacagcgcg tactggttgg atgtggagga gttcgaccgg
      541 cagtatgcgg tcgcccaggg gtcgccggat gacacgcgga tcgccaccag ctaccgggcc
      601 tcagtgctgc ggacatgcgt ggagctctat cggggtgact tcctcgccgg attcttcgac
      661 gattgggttg tcgccgaaca ggaccactac cgacagcgct acatgactgc gctcagtcgg
      721 ctcatcgaag ccaccaagtc tgccggcgcc tacgaagagg ccctggggta tgcccgacgt
      781 atgacccacc acgatcccct cagcgaggag gcacatcgcg aagtcatgag actgtgcttc
      841 ctgctcggtc ggacgggcga cgccgtcgag cagtaccgga gatgccgatc ggtgctcgaa
      901 gacgatctcg gagccgaccc gtctcctgcc accgtcgagc tctacgagaa gatcgtcagg
      961 cagaggcgtg ccgggatccg cgtcggcttc gaggagagca aacccgccct gtccatgcgt
     1021 tctgattctc ccttcgtggg tcgcgaggac gagcgtcggc ttctcatcga cgcggtggag
     1081 cgggttctgg cggggccggg tggagtggct ctgatcgaag gtgagcccgg cgtcggaaag
     1141 acgaggctgg cgaacgaggt tgtggacgac gccaagtgga ggggattcga ggtcagctgg
     1201 ggatcgtgtc gccccggtgc gttacgcccc ttcgcccccg tggtcgagat cctggagtcg
     1261 ctcaccccga tccgcctgga gcagctggcg gagcaggtag cccccatctg gttcaatcag
     1321 gcgcttcggc tcgccccgca gttggctcat cggttagatg gctccgagga gccggctccc
     1381 ttgcgtccag ccgaagagct gaccaggacc caagaagcgc tggttcacac tctcggggcg
     1441 ctggggcgga tcgcccccca tctcctggtc atcgatgatg ttcaatgggc cgaccgggac
     1501 actctgtccg tcctgaccca gcttgcctcc cgcctcgccg gcacccgggt cctggtactt
     1561 gcggtcttcc ggagcgagga ggctcggggt gagcccgagg tctgggacgt catcagggac
     1621 ctcgatcgtg tggcgggctt gggtcgggct gtcctctctc cgctgtcggt cttcgaactc
     1681 gacgagatgg ttcgtcgcat cctggaagtc ccccggttgg atccatcggt agctacacgg
     1741 cttcaccgtg agaccggcgg caacgcgctg ttcgcactcg agaccctgtt ggcgatgaga
     1801 gatcgcggaa ggctcgaggg aggcaatgac ctgctcgccg ggctcgaacc gcccatcaca
     1861 ggcaaagagc tgccggtcgc cccgagggtt cgggcggtga tcgagtcgcg gatctctctc
     1921 ctgggtgacg aggtggcaga ggtgtatcag gtcgcatcgg tcatcggaga tccggtgcgg
     1981 ctcgagctcc tcgaggcagc cgcggaactg gcgagaccgg tgatcctcga cgctgtcgac
     2041 gagctcctcc atcgcggact gctccgcgag gtcggcaaca gtgtctacag gctcgcacat
     2101 gaccagatcc gtcaggttgc ctacgaggcc atcgcgtctg atcggcggga acggacccac
     2161 ctggcgatag ccgagagcct cattcgactc gacgccgatc aggtcgagga aatcgggcac
     2221 cacttcttga tgggagggga ctacgacagg tcggcccgat atcttctcag agccggtttg
     2281 cgtgccgtcg agctcagtgc attcgccacc gcggccagcc atttgcagga ggctcgactt
     2341 gccgccatcg acgccgggtg gtcggacgcc gatagatacg cccttcttgg tcatctcgaa
     2401 gacgtgctcg gggttctcgg acgcaggcag gagagctccg cggtcatcga cgagatggaa
     2461 gatctggtgg atgccgtcac cgagcccgag ctggcccgtc ggcgatcctg gcttctggca
     2521 catgaaggtc gtctctcgga tgccgaagag atggctgctc gatccgtcgc cttggaggag
     2581 gaggcagtga gtgcggacct ggccgactca ttggtcgctc tgggcacgat ccggcgatgg
     2641 tcgggcaggc cgttggaggc agtctcggtg ctggaggatg cggtcgatgc ggcaggagac
     2701 aacgagcgtt tgcgggctcg ggccctcacg gagctggcca gcacgctctt cgcgttgcag
     2761 cgtcacgctg atgccacacc acttctcgag caggccgcct cgatccacga gcgtctgatc
     2821 gacctgcggg gccaggcaga ggtcggcggg atacgagctc gcgccctcgg cgagcaaggg
     2881 agacgagacg aggcgcagga gcagtacagc aacacgatcg aactctgcag gaggatcggc
     2941 taccggcacg gcgaggggat gaacctggtc aatctcgcca atctccatca gagacacggc
     3001 aacgccttgg ccgccctcga aggatatgac cgagccgcgg tcatcttcaa ggagttgaac
     3061 aacccccgcg gcgaggccgt tgtcctcgcc aatgcggctt cggcccggca cagcatcctc
     3121 ggagacgacc aacgcgccaa ggaggacgcg cagcgtgcca tgggtctctt cgaggggatt
     3181 ggcgaccttg gccatatggc ccagtgtgaa gagattctgg ccggaatcac cgcgcgttct
     3241 ggcgacctgg acaaggcgtt gagcatgatc gagcacagtc ttcgcaggct cgagggtgtt
     3301 ggagacgtgg ccctcgaggt tcagcatctt cgctcgctgg ctcttgttga gctggagttg
     3361 ggggactatg cgtcggccat ggccgccgtg gaccgggccg agcaacttgc cgccgacgtg
     3421 gcagttggtg acatcgccgt ggacttgtgg tcggtgcgag gcatcatcgc tatcgcgcgg
     3481 ggaaacacgt tcgagggtct gaccgagatt cgacacgccg tcgaacggct cggtcccggg
     3541 gtcgagcggc aatatctcat ccaccatcgc cacgcccggg cggcccacgc cgcgggagaa
     3601 cgcgaagagg cccgcctggc ggcgatgagg gcttcgaccg agttggagaa cgtcattgcc
     3661 ggcctcgatg ccgaggcccg tcgccaggcg atcgaaatgg ttcccgagca tcgctcgatc
     3721 gatgaatggg cccgcttcta ctccccatcg accatcgatg tcgctctccc ggcgactgtc
     3781 gctcctatgg gtcgacgttt ggagcctgaa gaaatggtcg agttgcggtg gacgatcgag
     3841 caccccgacg atcgctccat cgaagagggg atcgatcgac ggcgtcatcg gctgatgcgg
     3901 ttgctagacg aggcgggtgc ctatgcggcc tcaccgtctg tcgaagacct ggctcgggcg
     3961 ctgggtgtga gtgcatccac cgttcgtcgc gacatcgccc aattacgccg gagcggccat
     4021 gacgtcagaa ctcgaggtca gcgcaacgtg tcgtgacgga aggtcgggtg cccggccctc
     4081 ccatcactga gacaatgacg agacagcctc ccggcatagt cgattgcgac cgtgtctata
     4141 tggagctagg aggttcctca aatgcgtcgt cgccttaccg tgatcgctct ggccgtgatg
     4201 tccgccctcg caatcgctgc tcccgccctg gcctccgtaa aagacatgac ctgatctgcg
     4261 cagccggctg atgattcggc tcaggtcgga agatcggccg gcgcgagcct tcatctgggc
     4321 gtccgtagga cccgatttcg cgaaccgaat aaccggcctt cgggcaccca acccacggac
     4381 gcccagatga aagctctgtg atcgcgcgtc cctgaagcac acaccccagc cacgcaacgt
     4441 gagccgacgt gtgactttaa gtggcgcccc cggagattgc cctcgaatcc tggatctatc
     4501 ttctggatct atcttccatc gctctcgctc gagcggacgc ggccggtgtg gacatgctcg
     4561 atgggaagga agccatggac atcccagaag tggtgcgcaa gaaggcc
//
LOCUS       DASUTA010000304         3025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1902448, whole genome shotgun sequence.
ACCESSION   DASUTA010000304 DASUTA010000000
VERSION     DASUTA010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902448"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFZ80_06030"
     CDS             <1..389
                     /locus_tag="VFZ80_06030"
                     /inference="COORDINATES: protein motif:HMM:NF014077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX6287024.1"
                     /translation="ISLGQSDPWHWETAAQYAPAVARWMCRRKEGPEVLNVNVPGRPV
                     EEIRGVHWADLDEFGYFRVATADPEAGRLQFVVGPRTERSDPGCDTLLCDEGYVTLTP
                     LRTVEPAPFPPVDASAVAGKSVGVSS"
     gene            389..883
                     /locus_tag="VFZ80_06035"
     CDS             389..883
                     /locus_tag="VFZ80_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287025.1"
                     /translation="MGVGYGELAMTMTRPSMFSERRGFTIVGGQPSTVGPIVTRLSGS
                     RSSFVAMRVAAGHAMTRSCGMIILDETGDMGFPFDDDAVDERERSVAEGILANDHVSR
                     VPIGETGLREAILRGIEAEASLLVLGPEDILELTDTPALMTALIDAPFDVLLLASTGT
                     DRLR"
     gene            complement(880..1965)
                     /locus_tag="VFZ80_06040"
     CDS             complement(880..1965)
                     /locus_tag="VFZ80_06040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6287026.1"
                     /translation="MPGYFSGLGASEGLIGLLYSASALVSLSFRPLLGRVLDLTHRRT
                     VILVAAMANIVVLLGLSTTEVWGPWLWVLFLMQRTAQVALFTTMLTYGADSIPLKRRT
                     QGLAIFGLSGLIPIAIGGYFGDVVIEEFGFSGLFICAAAVSFLSWLVVWALPVLPVRG
                     RQPRRSFWAALAQRNLLPIWLATLLFTVGLESLFTFTRTFVDERDVGTAGLFFAVYGI
                     SAAVTRIVGGSMYDRLPHRPLLVGSLAAYGVGVGCMAIAQDVPLLFAAALITGTAHGA
                     AFPLLSSEVVNRARVSERGSAMATFTSIFDIALLAGAPAVGFLIDGFDYLVAFGVVSI
                     TLVIGSLAYRLWDRRIDPASIVAEEAV"
     gene            2147..2424
                     /gene="ffs"
                     /locus_tag="VFZ80_06045"
     ncRNA           2147..2424
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFZ80_06045"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(2493..>3025)
                     /locus_tag="VFZ80_06050"
     CDS             complement(2493..>3025)
                     /locus_tag="VFZ80_06050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6287027.1"
                     /translation="LDLGSGTGDTDFGNRSVIALDPVIEMLSLSPVPARVVGVGESLP
                     LADESVDGVFSAFVFRNLTSVDATLTEMARVLRPGAAAVVVDLGRPSNPVLRWLHRIG
                     TAVALPLVGLLFAGAPGEYWYLHKTLDSLPQPEELYAGRSLALEAVWRSGLFGFVYGV
                     RLRKAPAALEEEPEAA"
BASE COUNT          623 a          967 c          990 g          445 t
ORIGIN      
        1 ccatcagcct ggggcaatcc gacccctggc actgggagac agcggctcag tacgcacccg
       61 ccgtcgcccg atggatgtgc cgacgcaaag aggggccaga ggtactgaac gtcaatgtcc
      121 cgggccgccc tgtcgaggag attcggggcg tccactgggc ggacttggac gagtttgggt
      181 actttcgcgt agctaccgcc gaccccgagg cggggcgtct ccagttcgtg gtcgggcccc
      241 gcaccgagcg atcagacccg gggtgcgaca ccctcctctg cgacgagggg tacgtcacgc
      301 tgactccgtt gcgaacagtc gagcctgcgc ccttcccgcc cgtcgatgcg agcgcagtag
      361 ccggtaagag cgttggggtt tcgagctgat gggcgtcggg tatggcgaat tggcgatgac
      421 catgacccga ccatcgatgt tctccgagcg tcgcggcttc accatcgtcg gtgggcaacc
      481 cagcaccgtc gggccgatcg tcacccgact cagcggctcc cggagcagct tcgttgcgat
      541 gcgtgttgct gcgggccacg ccatgacccg cagttgcggg atgatcatcc tcgacgagac
      601 aggtgatatg gggttcccgt tcgacgacga tgcggtcgat gaacgcgaac gttccgtcgc
      661 cgaagggatc cttgccaacg accacgtctc cagggtgccg atcggggaga ccgggctccg
      721 cgaagcgatc ctgcggggta tcgaggcaga ggcgtcgctg ctggtgctcg gtcccgagga
      781 catcctcgag ttgaccgaca cgccggcact gatgaccgca ctcatcgacg cacccttcga
      841 tgtcctcctc ctggcatcga cgggcaccga cagactccgc tagaccgcct cctcggcgac
      901 gatcgacgcc ggatcgattc tccggtccca gaggcgatat gccagggagc cgatgaccaa
      961 cgtgatcgac acgactccga aggccaccag atagtcgaag ccgtcgatga ggaacccgac
     1021 ggctggagcc ccggccagca gtgcgatgtc gaagatcgat gtgaatgtgg ccatggcaga
     1081 gccgcgctcg ctgacccggg cacggttcac gacttcgctc gagagcaacg ggaaggccgc
     1141 accatgtgcg gttccggtga tcagggcggc agcaaacagc agtggcacat cctgggcgat
     1201 cgccatacag cccactccga ccccataggc ggcgagtgac cctaccaaga gcggtcgatg
     1261 tgggagacgg tcgtacatcg aaccgcccac tatccgggtg accgcggcag agatcccata
     1321 gacggcgaag aagagccctg cggtgcccac gtcacgctca tcgacgaatg tcctggtgaa
     1381 ggtgaacaac gattcgaggc caacggtgaa caagagggtc gccagccaga tcggcagcag
     1441 attgcgttgg gccagcgcag cccaaaacga gcgtcttggc tgacgacctc tgactggtag
     1501 caccggaagg gcccacacca ccaaccagga gagaaatgag accgcagcgg cgcatatgaa
     1561 cagcccggag aagccgaact cctcgatcac gacatcgccg aagtatccgc cgatcgcgat
     1621 gggtatcagg ccggacaggc cgaaaatggc caacccctgg gtcctcctct tcaggggaat
     1681 cgagtcggcg ccgtaggtca gcatcgtcgt gaacaaggcc acctgcgccg tccgctgcat
     1741 caagaagaga acccaaagcc agggacccca cacttcagtg gtggacagac ccaacagcac
     1801 gacgatgttg gccattgccg ccaccaggat gacagtccgg cgatgagtca gatcgaggac
     1861 acgacccaac aagggtcgga acgacaggga gaccagggcg ctcgccgagt agaggaggcc
     1921 gatgagccct tcggacgccc ccaggccact gaagtagccg ggcacgtgga cgagcagcgc
     1981 aaaagacagc tcctcgagga tcgccgccgt gaagagcctg gggaagtgtc ggccgaaacg
     2041 ctgggtcgca gtgagaatcc aggcaaggta acagtgggtc ggcagagggc atattcaccg
     2101 attcggtgtt gggatgaggt tgtggcgccg gtaccctcgt aggtgatcgt gtaagcgggg
     2161 cgctaggggt gccctgaacc cgaaaccctc ttcatgcggg gcccaatccc ccgcccgagg
     2221 gccacgacgc gtcggggcgc acagcccgcg acggcggtgt cggggtgatg acaatcgggt
     2281 cctgcgcggc gggacaccac gaaccgggtc aggtccggaa ggaagcagcc ctaagtggat
     2341 cgtctcgggt gctgcagggt ggcctggttc gagcctgcgg ccgccggcaa cgacgcccac
     2401 cgtgtctcga aggcggggtc acgatcgagc ccattccacg aacccggggt tcctcaggcc
     2461 ggatacggcc caaatccgct catctacggc aactacgccg cctccggctc ctcctccagg
     2521 gcagccggcg ccttgcggag acggactcca tacacgaacc cgaacagccc gctcctccac
     2581 accgcctcca gggcgaggga ccgtccggca tacagctcct caggctgagg cagcgagtcg
     2641 agggtcttgt gcagatacca gtactcaccg ggtgctcccg cgaagagaag accgacgagc
     2701 ggaagggcaa cagcggtccc gatccggtgc agccaacgca agacgggatt ggaggggcga
     2761 ccgagatcga ccacgactgc tgcggcccct ggtctcagga cccgagccat ctcggtaagc
     2821 gtggcgtcga cagatgtgag attgcggaac acgaaggcgc tgaacacacc gtcgactgac
     2881 tcgtcggcga gtggcaggct ctccccaaca ccgacgacac gagcaggcac cgggctcagg
     2941 gagagcattt ctatcaccgg gtcgagggcg atcaccgagc ggttcccgaa gtctgtgtca
     3001 ccggtgccac tccccagatc gagca
//
LOCUS       DASUTA010000305         3741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1514591, whole genome shotgun sequence.
ACCESSION   DASUTA010000305 DASUTA010000000
VERSION     DASUTA010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514591"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VFZ80_06055"
     CDS             complement(<1..537)
                     /locus_tag="VFZ80_06055"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6287028.1"
                     /translation="MTAIILDGIVLGLQFGLLAAGLTLVYGLGGVLNLAYGQIAVLSA
                     MVVLFSMDAGVPLTAAIALGLVAGGVIGLILDRTILRPIYRRQGEARVLLSLLLTLGV
                     AFVVDGFLTWRYPFEGLSLQIGGGPIELFGVPMRRGSIVGSIIILVVFGLLFAFFRGT
                     SLGRAVRSVIEDEEGSRLV"
     gene            complement(534..1232)
                     /locus_tag="VFZ80_06060"
     CDS             complement(534..1232)
                     /locus_tag="VFZ80_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018347940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6287029.1"
                     /translation="MLDVNGLAAGYQGNDVVHDISFHLDEGEAVMIIGSNGAGKTTLF
                     RAMCGLSQPSSGTVEFEGEDVTGLPAQRLARKGLGFVPAERHLFPRMTVHENLSLGAY
                     PKRPDPETMSVVFDLFPRLDERRRQHAGTLSGGEQQMLAVGRALMSKPRLLVLDEPTT
                     GLAPKLAHEAYEALAVLRGQGLTLLVAEQQVPLAMAFTDRGYVLENGVISLEGTSQAL
                     ASDPRVRSAYLGVT"
     gene            complement(1225..1965)
                     /locus_tag="VFZ80_06065"
     CDS             complement(1225..1965)
                     /locus_tag="VFZ80_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6287030.1"
                     /translation="MALLELDGVGKHFGGLPAVDGVTMRADDGEILAVVGPNGAGKST
                     LLKLIVGLESPSEGTISFDGDDITRAPTHRIRQKGIAMVQQTPRSFPNMTVLENATLG
                     AMFGLSSGIRDEVEATAIGYEMLDFVGLGDRRDDRVGNLNLHEQRFLELARAITGEPR
                     ALLLDEVMAGLNDAELDASVDIVRTIRDRLGITVIWVEHVMKAVLRLAERVVVLDFGK
                     VLTEGQPETVMRDRRVVEAYLGAEIEGA"
     gene            complement(1973..3271)
                     /locus_tag="VFZ80_06070"
     CDS             complement(1973..3271)
                     /locus_tag="VFZ80_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6287031.1"
                     /translation="MRTRPKSGVLALLAALALVVAACDGGTADTTEPDTSTTEAPTDT
                     TAAPTDTTAAPTDTTAAPSGEPIRIGALTDLTAFTPWAVNARDGMLLAAEEINAEGGV
                     DGRMIEIVIQDSANDADAGIEGFERLVEDGVVAVGGTISSTVGAAVAPLAEQAQMPYF
                     LIKSGTEAALTADSRYTFRTCLPAAPMTAGPVLQYAEEQGLTNVGVIVADYAWGQSFA
                     AAVESTFAGSDVDYGEIQVAPVPPDTDFTPFVRNLAGSEMVIATGHPPGNAAILALSA
                     DLVGDVPVTGAWTPPDLAVGAQPDVAIGRYADFACADYTSDGYAALAERYLAFSDNAM
                     MSDDAVAGFGIVTMVAEAVAEVGDDPVAIAEYMHGTSFDLEGYAHPMSWTEWGELAEA
                     QPIFFQITEGAAPGGLNEAGDWWIEVLTQSEPLEPYTPGS"
     gene            complement(3480..>3741)
                     /locus_tag="VFZ80_06075"
     CDS             complement(3480..>3741)
                     /locus_tag="VFZ80_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018461149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEX6287032.1"
                     /translation="IENRLMDHEAVHEAAVVSVVVDDISRIKASVILSDGREGNDQLV
                     RELQEWCKDTLLRYQYPHVVEFVDDFPRTATGKIQRFKLRGP"
BASE COUNT          680 a         1211 c         1172 g          678 t
ORIGIN      
        1 gaccagcctc gatccctcct cgtcttcgat gaccgagcgc accgccctgc ccaaggaggt
       61 gcctctgaag aaggcaaaga gaaggccgaa gacgacgagg atgatgatcg agcccacgat
      121 gctgccccgc ctcatgggca ccccgaagag ctcgatgggc ccgccgccga tctgcaggct
      181 caaaccctcg aatggataac gccaggtcag gaatccgtcg acgacgaacg ccactcccag
      241 cgtcagcaac aagctgagta agacacgtgc ctcgccctgc cggcggtaga tcgggcgcaa
      301 gatggtccgg tcgaggatga gtccgatcac cccacccgcg accaaaccga gagcgatggc
      361 cgccgtgagc ggtacgccgg catccatgga gaagaggacc accattgcag agaggaccgc
      421 gatctgcccg taagcgaggt tgaggacacc acccagccca tacaccaggg tgagccccgc
      481 ggccaggagg ccgaactgaa gcccgaggac gatgccatcg aggattatcg cggtcatgtc
      541 acaccgaggt acgcgctgcg gacgcgagga tcggacgcga gggcctggga cgttccctcc
      601 aagctgatga cgccgttctc caggacgtaa ccacggtcgg tgaaggccat cgccagggga
      661 acctgctgct cggcgacgag aagggtcaaa ccctggccac ggagcacggc caacgcctcg
      721 taagcctcat gagccagctt gggggccagc ccggtggtgg gctcgtcgag gaccaagagg
      781 cgaggcttgg acatgagggc gcggcccact gccagcattt gctgctcgcc accgctcaga
      841 gtccctgcat gctgacgacg tcgctcatcg agtcgtggga agaggtcgaa gacgaccgac
      901 atcgtctccg ggtccggtcg cttggggtag gcaccgagcg acaggttctc gtggacggtc
      961 attcgtggaa acagatgacg ttcggcgggg acgaagccaa gccctttgcg cgccagacgc
     1021 tgggcgggca gcccggtcac gtcctcaccc tcgaactcca ccgtccccga gctcggttgg
     1081 ctgagaccgc acatcgcccg gaagagagtc gtcttacctg ccccattgga gccgatgatc
     1141 atcaccgcct cgccttcgtc gagatggaag gagatgtcgt gcaccacatc gttgccttgg
     1201 tagccggcag ccagcccgtt gacgtcaagc accttcgatt tccgctccca agtaggcctc
     1261 gacgactctc cggtctctca tcactgtctc gggttggccc tctgtcagga ccttgccgaa
     1321 atcgaggacg acaacacgct cggccagtct caacacggcc ttcatgacgt gctcgaccca
     1381 gatgacggtg atgccgagcc ggtcgcggat agtcctcacg atgtcgacgg aggcatcgag
     1441 ctcggcgtcg ttgaggccgg ccatgacctc gtccaacagg agagcccgcg gctctccggt
     1501 gatggcccgt gccaactcga ggaatctctg ctcgtggagg ttgagattgc cgactcggtc
     1561 gtctcgccga tcgcccagac cgacgaagtc gagcatctcg tatccgatgg ccgtcgcttc
     1621 gacctcgtcc ctgattccac tcgaaagacc gaacatcgca ccaagggtcg cattctcgag
     1681 cacggtcatg ttggggaagg agcgcggcgt ctgctgcacc atcgcgatcc ccttctgacg
     1741 gatccggtgt gttggggctc gggtgatgtc gtccccgtcg aaactgatcg tgccctcgct
     1801 cggagactcg aggccgacga tcagcttgag cagggtgctc tttccagcac cattggggcc
     1861 gacgacggcg agtatttcgc catcgtcggc cctcatggtc actccgtcaa cggccggtaa
     1921 gccgccgaag tgcttgccga ccccgtcgag ctctaatagg gccacccgat gattacgacc
     1981 cgggggtgta aggctcgagc ggttcggact gggtcaggac ttctatccac cagtcgcctg
     2041 cctcgttcag ccccccgggc gccgcaccct cggtgatctg gaagaagatg ggctgagcct
     2101 cggccagctc accccattcc gtccagctca tgggatgggc gtagccctcc aggtcgaacg
     2161 aggttccatg catgtactcg gcgattgcca ccgggtcgtc cccaacctcc gcgactgcct
     2221 cggccaccat ggtcactatg ccgaagccgg cgacggcgtc gtcggacatc attgcgttgt
     2281 ccgagaatgc caggtagcgc tccgccagcg cggcatagcc gtcgctggtg tagtcggcac
     2341 aggcgaagtc ggcgtagcga ccgatggcga catcgggctg cgcgcccacc gccaggtccg
     2401 gcggtgtcca ggctcccgtc acgggcacgt ccccgacgag gtcagctgac aatgcgagga
     2461 tcgctgcgtt tcctggcggg tgaccggtgg cgatgaccat ctcggagccg gccaggttcc
     2521 gcacgaatgg tgtgaagtcg gtgtcgggag gaaccggcgc cacctggatc tctccatagt
     2581 cgacatcgga gcccgcgaac gtgctctcca cagcggcggc gaacgactga ccccaagcgt
     2641 agtcggcaac gatcacgccg acattggtga ggccctgctc ctccgcatac tggagcaccg
     2701 gccctgcagt catgggtgcg gcagggagac aggtgcggaa cgtgtacctg ctgtcggcgg
     2761 tgagtgcagc ctcagtgccc gacttgatca ggaaataggg catctgggct tgttcggcca
     2821 gaggtgccac cgcggcaccg acggtgctcg agatggtgcc tcccacggcg acaacgccat
     2881 cttccaccag acgctcgaag ccctcgatgc ctgcgtctgc atcgttcgcc gagtcctgga
     2941 tgacgatctc gatcatccgg ccatcgactc cgccttcggc gttgatctct tcggcggcga
     3001 gcagcattcc gtcccgggcg ttgacagccc atggcgtgaa cgcggtcagg tcggtgagag
     3061 caccgatcct gatcggctcg ccggaaggcg ccgccgtcgt gtcggtcggt gcagcggtgg
     3121 tgtccgttgg agcagcagtg gtgtcggtag gagcctcggt cgtcgaggta tccggctcgg
     3181 tcgtgtccgc cgtgcctccg tcacatgccg ccaccaccag ggctagcgcc gccaacagcg
     3241 ccagcacccc ggacttgggg cgtgtcctca ttgttcctcc tcgtttctgt tgtgctatgt
     3301 gtcatctctc ccggtgatca acgacactac gcacgctcag tgtcactttg cggtgacact
     3361 ccaccgacca tcgcactcgg ttcgctctgc gaacatatgt gcgacaggcg gtcaagctgc
     3421 gagagtacgt cctagggatc gtgaccgcaa caggtatcag gggttttgcc ggatgcacgt
     3481 cagggacccc tgagcttgaa ccgttggatc ttcccggttg cggttcttgg aaagtcgtcg
     3541 acgaactcga cgacatgcgg atattggtat ctgaggaggg tgtctttgca ccactcttga
     3601 agttccctca ccagttggtc gttaccttct cgcccgtcgg agaggatgac cgaggccttg
     3661 attcgggaga tgtcgtcgac taccacgctg accacggccg cctcgtgaac cgcctcgtgg
     3721 tccatgagac gattctcgat c
//
LOCUS       DASUTA010000306         2564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_996682, whole genome shotgun sequence.
ACCESSION   DASUTA010000306 DASUTA010000000
VERSION     DASUTA010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996682"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VFZ80_06080"
     CDS             <1..564
                     /locus_tag="VFZ80_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6287033.1"
                     /translation="NPYGWTKIVMERVLADVQAANQSWSVSLLRYFNPVGAHSTGLIG
                     EDPRGVPNNLMPYIARVAAGLLPRVRVFGGDYGTPDGTGVRDYIHVSDLAAGHVSALD
                     QKADVPGVHVYNLGTGRGHSVLEVIKTYEEESGQPIPYEIVERRPGDVAASWADVSKA
                     RDELGWVAERDLAAMCADSWNWQQKAR"
     gene            592..2277
                     /locus_tag="VFZ80_06085"
     CDS             592..2277
                     /locus_tag="VFZ80_06085"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010638410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEX6287034.1"
                     /translation="MSDLIERARIWIEADPDPGTRAELEAVISSGDREELQNRMNTVL
                     DFGTAGIRGVVGAGPGRMNRAVVIRTTYGLARHLGSTDRPVVVGFDARPTSKQFAEDT
                     CGVLVASGIEVLYFPTFTPTPLVAFAAKHLDAVAAVVVTASHNPPADNGYKVYGRQAS
                     QIVSPVDLEIQRNIADAPAADEIARIDGVFSGVSDLARPVPDDIVDHYWSEVSATRSR
                     HSGSRLSIVYTPIHGVGKATLFDIMGRAGHSEIEAVPEQAEPDGTFPTVAFPNPEEPG
                     ALDLALEKATRDRADLVIANDPDADRLAAVVPHEGRWRPLTGNEVGALLGEYLLSNDK
                     EPSTAIVASSIVSSPMLSKIAESFGARHEATLTGFKWIVMAGLELESRGEGRFIFGYE
                     EALGYTVGSIVRDKDGISAALLFSDLAADLADEGRTVIDRLHALWRRHGLWVSSQSSI
                     VRPDVPDQLTTAVERLADSPPDTVGGHRVVAVTDYRKDAGSRPPWLGEQALVELDLGA
                     MGRVLVRPSGTEPKLKVYVDLAGDPGDDPDASHREMSALASSIGEDLAGSLGV"
     gene            complement(2280..2459)
                     /locus_tag="VFZ80_06090"
     CDS             complement(2280..2459)
                     /locus_tag="VFZ80_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287035.1"
                     /translation="MFGPLNGVRTALVIAALLAALVSLIAGYPGAAAVLGAGVALHGL
                     GWLYLYLTRDRARVG"
BASE COUNT          493 a          791 c          834 g          446 t
ORIGIN      
        1 aatccttacg gatggaccaa gatcgtcatg gagcgagttc ttgctgacgt tcaagcggcc
       61 aatcagagct ggagcgtgtc gctgctcagg tacttcaatc cggtaggtgc ccactccacc
      121 gggttgattg gtgaagaccc acgaggtgtc cccaacaacc tcatgcctta catcgccagg
      181 gtggccgccg gcctgttgcc aagagtccga gtgtttggcg gcgactacgg aacacctgac
      241 ggcaccggtg ttcgcgacta catccatgtc agtgatctcg ctgctggtca tgtctccgcg
      301 ctggatcaga aggcagatgt gccgggagtc cacgtctaca acctggggac gggtcgaggt
      361 cacagtgtcc tcgaggtgat caagacctat gaggaggaat cgggccaacc gatcccatat
      421 gagatcgtgg aacgacggcc aggcgacgtg gccgccagtt gggcagacgt atcgaaggcc
      481 cgggacgagt tgggctgggt ggcggagaga gatctcgccg ccatgtgcgc cgactcctgg
      541 aactggcagc agaaggcccg ttgacagagc ggtagttacc ctcccagagc gatgagcgac
      601 ctgatcgaac gggcccgaat ctggatcgaa gccgaccccg accccgggac cagggccgaa
      661 ctggaggccg tcatctcctc cggtgaccgc gaagagctgc agaatcgcat gaacacggtt
      721 ctggacttcg gaacagcggg aatacgcggc gtggtgggtg ccgggcccgg aaggatgaac
      781 cgcgccgtcg tcatccggac cacttacgga ctggcacgtc atctggggag caccgaccgc
      841 ccggtagtgg tcgggttcga cgcccgacct acgagcaagc agttcgccga agacacctgc
      901 ggggtgctgg tggcttccgg catcgaagtc ctctacttcc cgactttcac gccgacgccg
      961 ctggtcgcct tcgctgcgaa acacctcgat gccgtcgccg cggtggtggt gactgcatct
     1021 cacaatccac ccgccgacaa cggttacaag gtgtacgggc gtcaggcgtc tcagatcgtc
     1081 tcgccggtcg acttggagat ccaacgaaac atcgcagacg caccagctgc tgacgagatc
     1141 gcacggatcg atggcgtctt cagtggggtc tctgacctgg cacgacccgt gccggacgac
     1201 atcgtcgatc actactggag cgaagtgtca gccaccaggt cccggcattc ggggtcaaga
     1261 ctaagcatcg tctacacccc catccacgga gtgggcaagg ccaccctctt cgacatcatg
     1321 ggccgggccg gccacagcga aatcgaggcc gttcccgaac aggccgagcc ggatgggacc
     1381 tttccaaccg tcgcgttccc caatcccgag gagccaggtg cgctggatct cgcgctggag
     1441 aaggcaaccc gggatcgagc cgaccttgtg atcgccaacg atccggacgc cgatcgtctc
     1501 gccgcggtcg tcccccacga aggtcgatgg cggcccttga cgggaaacga ggtcggggcc
     1561 ctgctcggtg agtacctgct ctccaacgac aaggaaccat cgacggcgat cgtcgccagc
     1621 tcgattgtct ccagtccgat gctgtcgaag atcgccgagt cctttggtgc gcgacatgag
     1681 gcgacgctca ccggcttcaa atggatcgtc atggccggtc tggagctgga gtcacgaggt
     1741 gagggacgat tcatcttcgg ctatgaagag gcactcggat acacagtcgg ttcgatcgtc
     1801 cgggacaaag atggcatctc ggcggcactt ctgttctccg atctggctgc ggacctcgcc
     1861 gatgagggaa gaaccgtgat cgaccggctg cacgcattgt ggcggagaca cgggttgtgg
     1921 gtcagctcgc agtcttcgat cgtccgcccc gatgtaccgg atcagctcac gacggccgta
     1981 gagcgactcg ccgacagccc tcctgacacc gtcgggggcc acagggtcgt ggcggtcacc
     2041 gactatcgga aggacgccgg ctccagaccc ccatggctcg gtgagcaggc gctggtagag
     2101 ctcgatctgg gcgccatggg ccgtgttctg gtgcgaccga gcgggaccga gccgaagctc
     2161 aaggtctatg tggatctggc cggagatccc ggtgacgacc ctgacgccag ccaccgcgag
     2221 atgagcgcgt tggcgtcatc gataggcgag gacctcgccg gttcgcttgg cgtgtgagat
     2281 cagccgaccc gggcccggtc tcgggtcaag tagaggtaga gccatcccaa accgtgcaag
     2341 gcaacaccag cgcccagcac tgcagcggct cccgggtatc cggcgatgag tgagaccagg
     2401 gcggcgagga gggcggcgat gaccagggcc gtgcggacac cgttgagtgg gccaaacatg
     2461 ggcatcaggt tacgccgtta gctcccaata catgcgaagg tgtcctccga tgtcccgtct
     2521 catgctggct tcggccgtgc tcgtcgtgat cgtcggctgc accg
//
LOCUS       DASUTA010000307         2053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_738515, whole genome shotgun sequence.
ACCESSION   DASUTA010000307 DASUTA010000000
VERSION     DASUTA010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738515"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFZ80_06095"
     CDS             complement(<1..196)
                     /locus_tag="VFZ80_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003983319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEX6287036.1"
                     /translation="MSGKLGQTPSQTVGPYFAMRIATGDEWRLAVPDGARRIVITGRV
                     LDGDGEPVPDALVETWQANPE"
     gene            complement(193..912)
                     /gene="pcaH"
                     /locus_tag="VFZ80_06100"
     CDS             complement(193..912)
                     /gene="pcaH"
                     /locus_tag="VFZ80_06100"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HEX6287037.1"
                     /translation="MSQTIIRHYGPRAYPDHPPFEYPAYKSTIKRNPKQDLLSIVETL
                     TETTGPGPVWTEIVEEDSDLTTNAGTGGQAIGERIIVTGRVLDENGTPVPNTVLEIWQ
                     SNAAGRYVHWKETNLKAPLDPNFIGVGQCKTGTDGVYRFTSVKPGPYPWGNHPNAWRP
                     SHIHFSVFGQSLGSRLVTQMYFEGDPLLTLDPIYQSAPEHSRTRMVAKYDHDVTEEGW
                     ATGWRWDIVLRGEESLLEVPE"
     gene            complement(909..1277)
                     /gene="pcaC"
                     /locus_tag="VFZ80_06105"
     CDS             complement(909..1277)
                     /gene="pcaC"
                     /locus_tag="VFZ80_06105"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007238767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HEX6287038.1"
                     /translation="MTSFEDGMEVRRRVLGDEHVDRATEAMTDLDRDFQAWITENVWG
                     GVWNRPGLDLKTRSIITIAILGALNHEELALHLRAAKNTGVSVDEITEILLHVAVYSG
                     VPAANRAFKVAKEIYEEGES"
     gene            complement(1305..>2053)
                     /locus_tag="VFZ80_06110"
     CDS             complement(1305..>2053)
                     /locus_tag="VFZ80_06110"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019567003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HEX6287039.1"
                     /translation="NGKPHSISCDFVIGCDGVRSSTREYVPSSRRREFERLYPFVWIG
                     VLSPTPPVNDELIYANHPEGFALCSMRNENLSRYYVQGDADDSPEDWPDERFWEALRT
                     RLPDGPAQRLVTAPTLETIVTPLRSWVMEPMRHERLFLAGDAAHIVPPTGAKGLNLAV
                     SDVVYLARALTEFYGSGSTGGLDGYSDRALARVWKAVRFSWWMTQTMHRFPGMSDDFD
                     RRLQQTELEYLFQSENARKSLAENYVGLPL"
BASE COUNT          339 a          647 c          654 g          413 t
ORIGIN      
        1 cttcgggatt ggcctgccat gtctcgacga gggcatctgg cacaggctcc ccgtcgccgt
       61 cgagcacccg accggtgatg acgatgcgtc tcgccccgtc gggcaccgcg agacgccact
      121 cgtcgccggt ggctatccgc atggcgaaat aggggccaac ggtctgggag ggagtctggc
      181 ccagcttgcc gctcattccg ggacctcgag caggctctcc tcaccccgga ggacgatgtc
      241 ccaacgccat ccggtggccc agccttcctc ggtcacatcg tgatcgtatt tggcgaccat
      301 tcgagttcgt gagtgctcgg gtgccgactg gtagatcggg tccagcgtga gcagcgggtc
      361 tccttcgaag tacatctgag tcacgaggcg gcttcccaga gattgcccga acactgagaa
      421 gtggatgtgc gatggtcgcc aggcgttggg gtggttgccc cacggatagg gccccggttt
      481 cacgctggtg aaccggtaga ccccatcggt gccggtcttg cactggccaa ctccgatgaa
      541 gttggggtcc aatggcgcct tgaggttggt ctccttccag tggacgtagc gaccggcagc
      601 gttcgactgc cagatctcca gcacggtgtt cgggaccggg gtcccgttct cgtcgaggac
      661 tcgaccggtg acgatgatcc gctccccgat cgcctggccg ccggtcccgg cattggtcgt
      721 gagatccgag tcctcttcga cgatctcggt ccaaaccggc cccggtcccg tggtctcggt
      781 gagagtctcg acgatcgaca gcagatcctg tttcgggttg cgtttgatcg tcgacttgta
      841 ggccgggtac tcgaacggcg ggtggtccgg atatgcgcgc ggtccgtagt gcctgatgat
      901 cgtctggctc atgactcacc ttcctcatat atctctttgg ccactttgaa tgctcgatta
      961 gccgccggga cacccgaata gacggcgacg tggagcagga tctcggtgat ctcatcgacg
     1021 ctgacgccgg tgttcttcgc cgccctgaga tggagggcca gttcctcgtg gttgagggcg
     1081 ccgaggatgg cgatggtgat gatgctccgc gtcttgagat cgagcccagg acggttccag
     1141 accccacccc agacgttctc ggtgatccag gcctggaagt cccggtcgag atcggtcatc
     1201 gcctccgttg cccgatcgac atgctcgtca ccgagcaccc ttcgtctcac ttccatgccg
     1261 tcctcgaagc tggtcatatt gatctcccta accatacgcc caggtcagag tggcagtccc
     1321 acgtagttct ccgccaggga ctttcgggcg ttctccgatt gaaacaggta ctcgagctcg
     1381 gtctgctgca accgccgatc gaagtcgtcg ctcatgccgg ggaatcgatg catcgtctgc
     1441 gtcatccacc acgagaagcg gaccgccttc cagacccggg ccagtgcccg gtccgaatag
     1501 ccgtcgagcc cgccggtgga gccgctgccg tagaactcgg tcagggcacg ggccagatag
     1561 accacgtccg agacagccag attgagaccc ttggcgcctg ttggcgggac gatgtgtgcc
     1621 gcgtcaccgg ccaggaacag gcgctcgtgc ctcatcggct ccatgaccca gcttcgcaga
     1681 ggggtcacga tggtctccag cgtcggtgcg gtgacgagtc tctgcgccgg accatcgggg
     1741 agtcgggtcc gcagcgcctc ccagaagcgc tcgtccggcc agtcctcagg tgagtcgtcg
     1801 gcgtccccct ggacgtagta gcgactcagg ttctcgtttc gcatcgagca cagtgcgaag
     1861 ccctcgggat gattggcgta gatgagctcg tcgttgaccg ggggcgtcgg ggacagcacg
     1921 ccgatccaca cgaacgggta tagacgctcg aattcgcgac gacggctcga gggcacgtac
     1981 tcccgggtgc tgctgcgaac tccgtcgcag ccgatcacga agtcacatga gatggaatgg
     2041 ggtttcccgt tgc
//
LOCUS       DASUTA010000308         3147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_478950, whole genome shotgun sequence.
ACCESSION   DASUTA010000308 DASUTA010000000
VERSION     DASUTA010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478950"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VFZ80_06115"
     CDS             complement(<1..324)
                     /locus_tag="VFZ80_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287040.1"
                     /translation="MSEVTLGPYRLGVPGEFGPRILSLRRGDGPEIFATSVEATIQRA
                     DGSYTFYGGHRLWASPEVPEFTYASEDNPCRVETASDVVVVRGSVDGAGYSKEISVTL
                     EDDRLR"
     gene            complement(326..1648)
                     /gene="xylA"
                     /locus_tag="VFZ80_06120"
     CDS             complement(326..1648)
                     /gene="xylA"
                     /locus_tag="VFZ80_06120"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEX6287041.1"
                     /translation="MTFFDQVTEVGPPGSDAQLSFKVYDPNRQVLGKRMEEQLRIAVC
                     YWHSFNWPGSDVFGQGTFDRPWLETTDDPMTAARQKMDAAFEFFTKLGVPFFCFHDVD
                     IAPTGATFAESAGNLDEMVGYAEEKMAETGVRLLWGTANLFGHPRYAAGAATNPDPEV
                     FAYAAAQVKHALEATHRLGGANYVLWGGREGYETLLNTDLKREQEQLARFLHMVADHK
                     RTIGFEGTLLLEPKPAEPTKHQYDYDCQSVHGFLVNYDLVDDYRVNIEGNHATLAGHS
                     FHHEVAYAVANGIFGSIDANRGDPQNGWDTDQFPNSVDEVALAVYEIMKGGGFSTGGF
                     NFDTKLRRQSIDRSDLFHGHIGGIDTLARALLVAEKLIEQETLSASKEERYRGWTEPL
                     GSGILDGSETLATLESSVMSGAIDPAPVSGRQELLENRVNEAIWSIED"
     gene            complement(1657..2364)
                     /locus_tag="VFZ80_06125"
     CDS             complement(1657..2364)
                     /locus_tag="VFZ80_06125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6287042.1"
                     /translation="MGDLQGRTALVTGASRGIGAAVARALDAEGVNLGLASRSGDDLG
                     IENAVARSADVRDPAALEDLAEATAARFGGIDILVINAGVGAYGPFLDLPFEHLEEMI
                     DVNVKGAIYSARAALPYLMRSDAADLVMLASEAGRRGLPLEAVYCSSKFAQVGLTRAL
                     DHELREQGVRCTTVAPGGVATDFAMGRGRTPDMPQLADMMRPEDVAEAVLYVLTRPRN
                     HRILEVAFRPMSEASMG"
     gene            complement(2402..>3147)
                     /locus_tag="VFZ80_06130"
     CDS             complement(2402..>3147)
                     /locus_tag="VFZ80_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018897224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6287043.1"
                     /translation="NVSFGGVRAVDGVTVDLYPGEIVGIVGGNGAGKSTLIKTLSGAQ
                     KPDSGQIFIDGKPVTIHGPMDAKNLGVETIYQTLALADNIDAPANVFLGREMLTPLRT
                     LDDSAMEDATRRVMARLNPRFDNFKVPVESLSGGQRQSVAIAKAVQFNARILIMDEPT
                     AALGPAETEVVRGLMRQLKEEGIGIFLISHDIHDVFDLADRISVMLQGKLVGTVDKDD
                     VTIDEVLAMIIIGKLPEEVTETELRDLHG"
BASE COUNT          532 a          967 c         1019 g          629 t
ORIGIN      
        1 ccggagacga tcgtcttcga gggtaacgga gatttccttg ctgtagccgg cgccgtcgac
       61 gctgccgcgg acgacgacga catcgctggc tgtctcgacg cgacaagggt tgtcctcgct
      121 ggcgtaggtg aattcgggca cctcagggga ggcccaaagt cgatgtcctc catagaacgt
      181 gtatgagccg tcggcgcgtt ggatcgtggc ctcgaccgat gttgcgaata tctcgggccc
      241 gtcaccgcgg cgaagactca ggattcttgg cccgaactcg cccggaaccc ccaaccgata
      301 gggaccgagc gtgacttcgc tcaactcagt cctcgatcga ccagatggcc tcgttcaccc
      361 ggttctcgag aagctcctgc cggcctgaca ccggcgccgg gtcgatcgca ccggacatga
      421 cgctggactc caacgtcgcc agcgtctccg agccgtccag gatgccgctg cccaacggct
      481 cggtccatcc tcgatatcgc tcttctttcg acgccgaaag ggtctcttgc tcgatcagct
      541 tctcggccac gagcagagct cgagccagcg tgtcgatgcc accgatgtgg ccgtggaaga
      601 gatcggagcg gtcaatcgac tgacgtctca gcttggtgtc gaagttgaag ccgccggtgc
      661 tgaacccgcc gcctttcatg atttcgtaga cagccagggc cacttcgtcg accgaattgg
      721 ggaactgatc ggtgtcccag ccgttctgcg ggtcgcctct gttggcgtcg atcgaaccaa
      781 atatcccgtt ggcgaccgcg tatgccacct cgtgatgaaa cgagtgaccg gccagggtgg
      841 cgtggttgcc ttcgatgttg actctgtagt catcgaccag gtcgtagttg accaggaagc
      901 cgtgtaccga ttgacagtcg tagtcgtact gatgcttggt cggctcggcc ggctttggtt
      961 cgaggagcag ggtgccctcg aaaccgatcg tccgcttgtg gtcggcgacc atgtggagaa
     1021 atcgggcgag ctgttcctgt tctcgtttca ggtctgtgtt gagcagggtc tcgtagcctt
     1081 cgcgaccgcc ccagaggacg tagttggctc cgccgaggcg atgcgtcgcc tccagggcgt
     1141 gttttacctg ggcagcggca taggcgaaga cctcgggatc ggggttcgtg gcggcgcctg
     1201 ccgcatatcg gggatgtccg aagaggttgg cggtgcccca gaggagtcgg acgccggtct
     1261 cggccatctt ctcctctgca tagccgacca tctcgtcgag gtttcccgcc gactcggcga
     1321 aggtcgcccc cgtcggcgcg atgtcgacgt cgtgaaagca gaagaacggc actccgagct
     1381 tggtgaagaa ctcgaaggcg gcatccatct tctgtcgggc agcggtcatg gggtcatctg
     1441 ttgtctcgag ccagggacgg tcgaaggtcc cttgcccgaa cacgtcggag cccggccagt
     1501 tgaacgagtg ccagtagcag acggcaatgc ggagctgttc ctccatcctc ttcccgagca
     1561 cctggcggtt cgggtcgtag accttgaagg agagctgcgc atctgagccg gggggcccta
     1621 cctcggtcac ctgatcgaag aaggtcatga gcgctctcaa cccatcgatg cttcggacat
     1681 cggccggaag gcgacctcga gtatccgatg gttgcggggc cgagtcagca catagagcac
     1741 agcctcggcc acatcctctg gtctcatcat gtctgccagc tgcggcatgt caggcgtccg
     1801 ccccctcccc atggcgaagt cggtggcgac accacccggg gcgaccgtgg tgcaccgaac
     1861 accctgttcg cgcaactcgt ggtcgagtgc tctggtcagg ccgacctggg cgaacttcga
     1921 tgagcaatac acggcctcga gcgggaggcc gcgtctccct gcctcgctgg ccagcatgac
     1981 cagatcggct gcatcggacc tcatcagata ggggagtgcg gcccgcgccg agtagatggc
     2041 ccctttgacg ttgacatcga tcatttcttc gagatgctcg aagggaaggt cgaggaacgg
     2101 gccatatgcg ccgaccccgg cattgatgac gaggatgtcg atgccgccga accgagcggc
     2161 agtcgcttct gcgaggtctt ccaaagccgc gggatcgcgc acgtcggccg acctggccac
     2221 ggcgttctcg atgccgaggt cgtcgccact ccgcgaggcc aaccccaggt tgacgccctc
     2281 ggcgtcgaga gccctggcta ccgcggcgcc gatgccgcgg ctggcgccgg tgacgagagc
     2341 cgttcttcct tgcaggtcgc ccatgtcggg tacctccttt gcgaattgag atggttcggg
     2401 actacccgtg gaggtcgcgg agctcggtct cggttacctc ttcgggcagc ttcccgatga
     2461 tgatcatggc gaggacttcg tcgatcgtga cgtcgtcttt gtcgaccgtc ccgacgagct
     2521 ttccctggag catgacgctg atgcgatcgg ccaggtcgaa cacgtcgtgg atgtcgtggc
     2581 tgatgagaaa gatgccgatc ccctcttcct tgagctgtcg catcagcccg cggacgacct
     2641 cggtctcggc agggcccagc gctgcggttg gctcgtccat gatgaggatg cgagcgttga
     2701 actggactgc tttggcgatc gccaccgact ggcgctgtcc tccggagaga gactcaacgg
     2761 ggaccttgaa gttgtcgaat ctcgggttga gtcgtgccat cacccggcgg gtggcgtcct
     2821 ccatcgccga gtcgtccaag gtgcggagcg gggtgagcat ctcgcgaccg aggaagacgt
     2881 tcgccggtgc gtcgatgttg tcggcgaggg cgagggtctg gtagatggtc tccacgccga
     2941 gattcttggc atccatcgga ccgtggatgg tcacgggttt gccgtcgatg aagatctgac
     3001 ccgagtcggg tttctgcgct ccagacagcg tcttgatcag cgtcgatttg ccggcgccgt
     3061 ttccaccgac gatgcccacg atctcgcccg ggtacaggtc cacggtgacg ccatcgaccg
     3121 cgcgaacgcc accgaaggag acattga
//
LOCUS       DASUTA010000309         2555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1129471, whole genome shotgun sequence.
ACCESSION   DASUTA010000309 DASUTA010000000
VERSION     DASUTA010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129471"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VFZ80_06135"
     CDS             <1..576
                     /locus_tag="VFZ80_06135"
                     /inference="COORDINATES: protein motif:HMM:NF037625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6287044.1"
                     /translation="ITGTMSRAQATRHRLQTTAFELFTSKGFDNVTVEEIAAAAGVSH
                     MTFYRNFPTKEAVVLDDPYDPLVFEAVAGQQPGIPAMEKVRRAVLEAFGRLDEPEDRT
                     MRQKIRLAANHPGLRARVWENNLRTEGIIVDALVGGGTSRLEAAVAAGAVLGALTAAL
                     FEWALDDSGGPLGERIGFALDVLATPTVGAR"
     gene            573..1307
                     /locus_tag="VFZ80_06140"
     CDS             573..1307
                     /locus_tag="VFZ80_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6287045.1"
                     /translation="MTAMLHAQGLVRSFGDERAVDGVDLVVDQGEVHAIVGLNGAGKT
                     TLMRLLLGMLRSDQGTAFIGGTDVTSAGASTWGNVGYLVETPFSYPELTTRENVTVAG
                     ILHGLEKAALESAVTEVIAMLELGHWADRRARGLSMGNRQRLGLAAALVHQPAVLLLD
                     EPANALDPAGVVLVRDLLRDLAGRGAAILVSSHHLDQLARVADVITVMHRGRVVGGLN
                     PTEHDLEQRFFDLVRVADERIPLEQR"
     gene            1304..2071
                     /locus_tag="VFZ80_06145"
     CDS             1304..2071
                     /locus_tag="VFZ80_06145"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6287046.1"
                     /translation="MIRAVQVELLKFRRSPVVIVSTLLMVVLLPAIGLGMFSVASEAR
                     AGPLAAKVEGLLIGEGWSAYLGLISQVAAAAAFVGVGVVTAWTFGREHSDKTFPSLFA
                     LTVSRRSIAMAKFGVLCIWVLVLGAVVTVFAVAAGAVVGVGSSDGGDVGAPLLRLLFV
                     VTTTGLLALTAGYVASVGRGYLPAVGAIVIVVAAAQISVLFGVGGWFPFALPGLVAIT
                     GSEAAPALSWMQFALVPVVVVAVVWLTVRWWKRAEVV"
     gene            2123..>2555
                     /locus_tag="VFZ80_06150"
     CDS             2123..>2555
                     /locus_tag="VFZ80_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene cyclase"
                     /protein_id="HEX6287047.1"
                     /translation="MDQVMEWLLDSDPAIRWQVMRDLTGDRRASTERSRISEEGWGSR
                     LLAAQTPDGMWGGGAYSPKWISTTYSLLLLRHFEPDPSDERVVDAVDRVRHGVDMRGP
                     FFEYSREMCITGMVLALRSYFFADPSDPPQVGYVTENQRDDG"
BASE COUNT          409 a          778 c          870 g          498 t
ORIGIN      
        1 attacgggta ccatgagccg agctcaagcc accaggcacc gtctccagac aacggcgttc
       61 gaactcttca cgagcaaggg tttcgacaat gtcaccgtgg aggagatcgc ggcagcagcc
      121 ggggtgtcgc acatgacctt ctatcgcaac ttccccacca aggaggcggt ggtcctcgac
      181 gacccatacg accccctcgt cttcgaggcg gttgccggcc agcaaccggg gatccccgcc
      241 atggagaaag tgcgacgagc ggtcctggag gccttcggtc gactggacga gcccgaagat
      301 cgaacaatgc gccagaagat cagactcgcc gcgaaccacc ccggtctccg ggctcgggtg
      361 tgggagaaca acctgcgcac agaggggatc atcgtcgatg ccctggtcgg tgggggcaca
      421 tcgagactgg aagcggctgt cgccgcgggg gcggtgctcg gggcactgac tgctgccctg
      481 ttcgagtggg ccctcgacga ctctggcgga ccgcttggcg agagaatcgg attcgccctg
      541 gatgtgctcg ccaccccgac agtgggggcg agatgactgc catgctgcac gcacagggcc
      601 tggttcggtc tttcggcgac gagagggcgg tggacggagt cgacctcgtc gtcgatcagg
      661 gagaggttca cgcaatcgtc ggtctcaacg gggcagggaa gacgactctg atgcgtcttc
      721 tcctcggcat gctccgctca gaccaaggaa cggctttcat cggcgggacg gacgtgacat
      781 ctgctggagc gtcgacctgg ggcaacgtcg gctacctggt ggagactccc ttctcctatc
      841 ccgagctcac cacccgggag aacgtcaccg ttgccggcat cctccatggc ctcgagaagg
      901 ctgcgttgga gtcggcggtg accgaggtca ttgccatgct cgagctgggg cattgggccg
      961 atcgacgagc ccgtggtctc tctatgggaa acagacagcg cttgggactg gccgcggctc
     1021 tcgtccatca gccggcagtc cttctcctcg acgagccagc caacgcgctc gaccccgccg
     1081 gtgtcgtgct ggtacgcgat ctgctccgtg atctcgcggg acgaggagca gcgatcctcg
     1141 tctccagcca tcacctcgat cagctggccc gagtcgccga cgtgatcacc gtcatgcatc
     1201 ggggtcgggt ggtcggtggg ctcaacccca cagaacatga cctcgagcag cgattcttcg
     1261 acctggtgag ggtcgccgat gagcggatcc cgctggagca gcgatgatca gggccgtgca
     1321 ggtcgagttg ctcaagttcc gccgatcccc ggtcgtcatc gtctctacct tgttgatggt
     1381 tgtgttactg cccgcgatcg ggctcgggat gttctcagtc gcctcggagg cgagggccgg
     1441 gccgttggcg gcgaaggtcg agggactcct gatcggagaa ggttggtcgg cctatctcgg
     1501 actgatctct caggtggcag cggcagccgc cttcgtaggc gtcggggttg ttaccgcttg
     1561 gacgttcggc cgcgagcact cggacaagac attcccttcg ctattcgctc tgaccgtgtc
     1621 acgtcgctcg atcgccatgg ccaagttcgg agtcctctgc atttgggtgc tggtccttgg
     1681 agctgtggtg acagtgttcg cagtggcggc cggcgcagtg gttggtgtcg gatcctctga
     1741 cggcggcgac gtgggggctc cacttttgcg gctccttttc gtggtgacga cgacggggct
     1801 actcgccctc accgccgggt atgtggccag cgtcggacgc ggctatctac cggctgtggg
     1861 cgccatcgtc atcgtcgtgg cagcggcgca gatctcggtc ctgttcggcg tgggaggttg
     1921 gtttccgttc gccctgcccg gtctggtggc tatcaccggg agcgaggcgg cgcccgcatt
     1981 gtcctggatg cagttcgcgc tcgttcccgt cgtcgtagtc gcggtcgttt ggctgacggt
     2041 tcgttggtgg aagagggcgg aggtggtctg acagaaccgc agcggagacc aaccggccag
     2101 tgtgaggtgc gacaatccca gcatggacca ggtcatggaa tggctactgg actcggatcc
     2161 cgcgatcaga tggcaggtga tgcgggatct gaccggcgac cggcgggctt cgacggagcg
     2221 ctcccggatc tccgaggagg gttgggggtc acgactgctc gccgcccaga cccctgacgg
     2281 gatgtggggg ggtggcgcct acagcccgaa gtggatctct accacctaca gcctgcttct
     2341 actccgtcat ttcgagcccg acccgagcga cgagcgcgtg gtggatgcgg tggatcgggt
     2401 gcgtcacggc gtcgacatgc gggggccatt cttcgagtac tccagagaga tgtgcatcac
     2461 cgggatggtc ttggccttgc gctcctactt cttcgcagat ccctccgacc ctccacaggt
     2521 cggctacgtc accgagaatc agcgcgacga tggtg
//
LOCUS       DASUTA010000310         7106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_93179, whole genome shotgun sequence.
ACCESSION   DASUTA010000310 DASUTA010000000
VERSION     DASUTA010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7106
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93179"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="VFZ80_06155"
     CDS             <1..1288
                     /locus_tag="VFZ80_06155"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX6287048.1"
                     /translation="MNYEQAIAYVDKHIGVGGEPGLDRMRDLLDLMGRPHEGYPVIHV
                     AGTNGKTSTSRLATLILVAHGLTTGTYTSPHLQHIEERLSVNGRVSTSEEFALAVSDV
                     AAFADLREDAGEVPNTYFELTTASAFAYFADQAVNAAVIEVGLGGRLDATNVVDAEVC
                     VVTSIGKDHTEFLGEDLASIAAEKLAIAGSSSILVTGPLPEEALDEANRKARELGIQH
                     RRYDRDYGIEAYERGVGGWLVTLKGAEATYEDIFLPLHGRYQLTNLAVAVAACEALVG
                     RRLDTKALRDAAAVATAPGRMEPLASSPFVIVDGAHNADGVRTLVESLQEEYPTTKWH
                     ILLAVMGEKNVDLMIELLAPIATGIVTTAVDSERALPALQLAEKVTQHLPGVPVLVSE
                     SVEYGLDMVRAEAGAEGAVLVTGSLYMVGEVREILT"
     gene            1335..1739
                     /gene="ndk"
                     /locus_tag="VFZ80_06160"
     CDS             1335..1739
                     /gene="ndk"
                     /locus_tag="VFZ80_06160"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013859508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX6287049.1"
                     /translation="MPVEHTFIMVKPDGVERGLVGEVISRFERKGLKLEKIRGLTISD
                     DLARTHYAEHVDKPFFSDLLEFITSGHVVAMEWSGEDAIAVGRRIMGATNPREAAPGT
                     IRGDFGLEVTENLVHGSDGPDSAQRELELFFG"
     gene            1935..2966
                     /locus_tag="VFZ80_06165"
     CDS             1935..2966
                     /locus_tag="VFZ80_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX6287050.1"
                     /translation="MAETLLSFAGQDMAIDLGTANTLVYVRGGGIVLNEPSVVAINSQ
                     NNKALAVGMEAKRMIGRTPANITAIRPLRDGVIADFDITEKMLRYFIQKVHRRKWARP
                     RIVVCVPSGITPVERRAVEEAAYHAGARRAYTIEEPMAAAIGSGLPIGEPSGSMVVDI
                     GGGTTEVAVISLGGIVVSRSIRVGGDELDSAIIDWVKKEHNVLVGERTAEQLKIAVGS
                     AWPYSDEPSAEVRGRDLVTGLPKTILLSSQEVREAIEEPTQAVVDAVKYTLDKTPPEL
                     AADIVERGIVLTGGGALLVGLDHRLAHETGMPIVTAAQPLQSVVLGSGKCLEEFDTLH
                     NVLVSSARP"
     gene            2989..3912
                     /gene="mreC"
                     /locus_tag="VFZ80_06170"
     CDS             2989..3912
                     /gene="mreC"
                     /locus_tag="VFZ80_06170"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX6287051.1"
                     /translation="MLSRQSGDRSVPTLITLLVIGVLLMTFDIRSEGQGVTSVLRQGT
                     QTIVAPLQEAAAFVVNPVLDLVDSLANIATLRETNVALQREVAELEAAIIALDDDMAR
                     LEFYEQLYDLETTGTDIGRTVAEVIGRPDPLDNALIIDKGTNEGIAVGQPVIDTNGYV
                     VGSVQQVTGGTARVVPITVGADGVAVTVGEQLGVLTPQINDNILRLEVNPARQPVLVG
                     DQVVTSSQSVRFPSGLPVGEVILDAAPGGAATLSTLVEPYMNPDNIRVVVVLAWPPDP
                     VAAVTEETTTTTSTTSTTLGGSTTSTTGGDG"
     gene            3912..4415
                     /gene="mreD"
                     /locus_tag="VFZ80_06175"
     CDS             3912..4415
                     /gene="mreD"
                     /locus_tag="VFZ80_06175"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX6287052.1"
                     /translation="MRLRTVLVVATLLLVAVVVQTTLFSQTEVFVPDLVMLVVIFLSL
                     TRIRPEAVLGIAFLGGLAVDLLGSSVLGLRAMVFTIVAYLAIRTRERADLGRVVVGLW
                     VGLLTLAGVLLILIVGTLFGQTVLLGDGVVGLLLLVPAANTILAWIFAPMVVRLIDRD
                     TTAFRYA"
     gene            4445..6376
                     /locus_tag="VFZ80_06180"
     CDS             4445..6376
                     /locus_tag="VFZ80_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX6287053.1"
                     /translation="MAFIAMFSIIGLRLWFVQVAQGPAIAQAAEEQTWLSKTTHAPRG
                     DIFDRNNHLLVTSRLTPAVFIDRTFVQPDDQNILIQRLSALLAMDPADLTHLYDEAGV
                     NGRFEVATVDNEVANQISEQLGSLPGVEIVNVPERVYLSGDTLAHVIGHLGLPDQADL
                     DAREDLDPTARIGKLGVERSYDDVLQGESGSVEYRVRLGEIIEQRPAIDPIPGNSLIL
                     TTDSDLQQVVELALEEGIRLSNEVKAAAPPDEEFFTETKRAAAVVLDPQTFDVLALAS
                     VPDFDPALFVSGIETEKFDELNESLAFNNLAVSGQFAPASTFKAITYTAFVEEDLPLP
                     EDTDFIDPSAKEVNCDGEWVFTLQDGSPQRKFDWYYPRTLGWLDLHGALENSCNKFFW
                     SIGLGAWQNRATVGETVLQDWAMDLGYGSETGIDLGSEAAGIVPTPALFEEWAEYQRE
                     NPDEPPRLDADRLDPTLVSPFFGGDLMDFAIGQGAFTATPLQAAVSYAILANGGRVME
                     PRVVDKIISPDGELIEDVSSEVVRTVPISDTTRQQLLADLNKVVTTGTAAAAFESFGP
                     GLELVAGKTGTTQVSQTRDDHAWFIGLAPIDDPQFVVAVVVEEGGSGGAIAAPVARHI
                     LQYLMGNEPTEIIAGDQTD"
     gene            6376..>7106
                     /locus_tag="VFZ80_06185"
     CDS             6376..>7106
                     /locus_tag="VFZ80_06185"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX6287054.1"
                     /translation="MTAVLTRIREVAVVRRNNRTDWILVGTVLLLSGFGLLMIYSATR
                     SVGTFSMERQMIFVAAGLIIFAIVSNIDYREYRSIVPAASIIILLLLAAVFFFPEING
                     ARRWIPIGFFALQPAEFAKIVVIVALASLLAPPASREDLMSGPRQLSWAKVGQALLVV
                     AVPGALIYLEPDLGTTLVFGFIVFGMLFVAGASWRQLTALATGVGVAGLLIWRLDLLQ
                     PHQLNRLEVLWNPDIDPQGIGYQLR"
BASE COUNT         1289 a         2268 c         2223 g         1326 t
ORIGIN      
        1 tatgaactac gaacaggcga ttgcctacgt cgacaagcac atcggggtgg gtggggagcc
       61 aggtctcgac cgaatgcgag accttctcga cctcatgggt cgtccccatg agggctaccc
      121 ggtgatccat gtcgccggga caaacgggaa gacctcgacc tcgcggctcg ccacactcat
      181 cttggtcgcc cacggcctga ccaccggcac ctacacctca ccgcatttgc agcacatcga
      241 ggaacgactg tcggtgaatg gtcgggtttc gacgagtgag gagttcgcct tggccgtgtc
      301 ggacgtggct gctttcgccg atcttcgtga ggatgccggt gaggtcccaa acacgtattt
      361 cgagctgacg actgcttccg cctttgccta cttcgccgac caggcggtca acgccgctgt
      421 catcgaagtg ggcctcggtg ggcgcctcga cgccaccaac gtcgttgacg ccgaggtgtg
      481 cgtcgtcaca tcgatcggca aggaccacac cgagttcttg ggtgaggacc tggcgagcat
      541 cgccgccgag aagttggcga tcgccggttc gagctcgatc ctcgtgaccg ggcctctgcc
      601 cgaggaggcc ctcgacgagg ccaacagaaa ggcgcgcgag ctcggcatcc agcatcgtcg
      661 gtacgaccgc gactatggca tcgaggcgta tgagagaggt gtggggggct ggctcgtcac
      721 cctcaagggc gccgaggcga cctacgagga catcttcctc ccgctccacg ggcgatatca
      781 actgaccaac ctcgcggtgg ccgttgccgc ctgcgaggcc ctggttggtc ggaggctcga
      841 caccaaggct ctgcgagacg cagcagcggt ggcaaccgcg ccaggaagga tggagccgct
      901 ggcgtcgtcg cccttcgtga tcgtcgacgg tgcccacaac gccgacgggg ttcggactct
      961 cgtcgagtcg ctgcaggagg agtacccgac aaccaaatgg cacattctct tggcggtgat
     1021 gggcgagaag aacgtcgatc ttatgatcga gctgctggcg cccatcgcca ccggcatagt
     1081 cacgaccgct gtcgactcgg aaagagctct tcccgctctc cagttggcgg agaaggtgac
     1141 acagcacctg ccaggggttc ccgtgctggt gtcggagagc gtcgagtacg ggctggacat
     1201 ggtcagggca gaggccggtg ccgaaggcgc tgttctcgtc acgggatcgc tctacatggt
     1261 gggcgaggtg cgggagatcc tcacctgaca cccgacttcg ccgaagacgg ccgcgccgaa
     1321 taccgtgctc aggcatgcca gtcgaacaca ccttcatcat ggtcaagccc gacggggtcg
     1381 agcgcggtct cgtcggagag gtgataagcc gattcgagcg caagggactg aagctcgaga
     1441 agattcgggg cttgaccatc agcgacgacc tcgccagaac ccactacgcg gagcacgtcg
     1501 acaagccttt cttctctgat ctcctcgaat tcatcacatc ggggcacgta gtggccatgg
     1561 aatggtccgg tgaagacgcc atcgccgtgg ggcggaggat catgggtgcc accaaccccc
     1621 gcgaggcggc cccggggacg atcaggggcg actttggcct cgaggtgact gagaacctgg
     1681 tccatggttc cgacgggcct gacagcgccc aacgcgagct cgaactgttc ttcggctgaa
     1741 cgcgacctcc ggtagttgga gccgcatttg agcccaacta cctcgaaaga gaagggcgga
     1801 cctgtctccg agcgactttc gaggcgatta gacccgcatt cggttctaac aaccggaggt
     1861 cgcggtgacc acctctgtgc agaagatggc ccacacctct acactccgta ggtccgcccg
     1921 ggagtcttcc ctcaatggct gaaacacttc tctcattcgc tggccaggac atggccatcg
     1981 acctcgggac cgctaacacg ctggtttacg tccggggtgg tggcatcgtg ctcaacgagc
     2041 cgtccgtcgt cgccatcaac tcccagaaca acaaggcgtt ggccgtcggc atggaggcaa
     2101 agcggatgat cgggaggacc ccggccaaca tcaccgcaat ccggccgctc cgcgacggtg
     2161 tcatcgcaga tttcgacatc accgagaaaa tgctgcgcta cttcatccag aaggtgcacc
     2221 gacgcaaatg ggcccgtcct cgaatcgtcg tctgtgtccc gtcaggaatc acaccagttg
     2281 agcgacgcgc cgtcgaggag gccgcctatc acgccggggc ccgtcgcgcc tacacgatcg
     2341 aagagcccat ggccgcggcc atcggctcag ggcttcccat cggcgagccg tccggctcga
     2401 tggtcgtcga tatcggtggt ggcaccacgg aggtggcggt catctcgctc ggcggcatcg
     2461 tcgtatcccg ttcgattcgc gtcggaggtg acgagctcga ctccgccatc atcgactggg
     2521 tcaagaagga gcacaacgtg ctggtcgggg agaggacggc cgaacagctc aagatcgcgg
     2581 tcgggtcggc ctggccgtac tcggatgagc cgtcggcaga ggtccgagga agagacctgg
     2641 tgaccggtct gcccaagacc atcctcctct cttcacagga ggtcagggag gcgatcgagg
     2701 agcccaccca ggcggtcgtc gacgcggtca agtacacgct ggacaagacg cctcccgagt
     2761 tggccgccga catcgtcgag cgtggaatcg tgctcactgg aggaggagcg ctcttggtgg
     2821 ggctggacca tcgtctggcc catgagacgg ggatgcccat cgtgactgct gcccaaccgc
     2881 tgcagtcggt ggtgctcggc tcggggaaat gcctggagga gttcgacacg ctgcacaacg
     2941 tgctcgtgtc atccgcccgg ccgtgactct gcagtcccgt cgcatgtcat gctgagtcga
     3001 caatccggag atcgctcagt ccccacactc atcacccttc tggtcatcgg tgtgctgctg
     3061 atgacattcg acattcgatc ggagggacag ggggtgacca gcgtccttcg ccagggcacc
     3121 cagacgatcg tcgccccgct gcaggaggca gcggcgttcg tggtgaatcc ggtactcgac
     3181 ttggtcgact cgctggccaa tatcgccaca ctccgggaga ccaacgtcgc gctgcagagg
     3241 gaggtcgccg agctggaagc cgcgataatc gcgctcgacg acgacatggc ccgtctcgag
     3301 ttctacgagc agctctacga cctggagacc acagggacag acatcggtcg aacggtggcc
     3361 gaggtcatcg ggcgccctga cccactcgac aacgctctga tcatcgacaa gggcaccaat
     3421 gagggcatcg cggtgggtca gcccgtcatc gacaccaacg gctatgtcgt gggttctgtg
     3481 cagcaggtca ccggtggaac ggcacgggtc gttcctatca ctgtgggtgc cgacggggtg
     3541 gcggtgacag tgggggaaca gctgggggtg ctcacacctc agatcaacga caacatcctc
     3601 agactggagg tcaatccggc gcgtcagccc gtgctcgtcg gcgaccaggt ggtcacgtcg
     3661 tcgcagtcgg tcaggttccc ctctggctta cctgttggcg aggtgatcct cgatgcggca
     3721 ccgggtggcg ctgccaccct gagcacgctg gtggagccat atatgaatcc ggacaacatc
     3781 cgggtggtgg tcgttctcgc ctggcccccg gatccggtgg ctgcagtcac ggaggagacg
     3841 acgaccacca cgagcacgac ctcgacgact ctcggcggct cgacgacgtc gaccaccggt
     3901 ggtgacggct gatgcgactc aggacagtat tggtcgtcgc cactctgctt ctcgtagctg
     3961 tcgtggttca gacgacgctg ttcagccaga cggaggtgtt cgttcccgac ctggtgatgc
     4021 tcgtggtcat atttctctcg ctgacacgaa ttcgacccga agcggtactc gggatcgcct
     4081 ttctgggagg cttggcggtc gacttgctcg gatcgtcggt cttgggcctg cgggcaatgg
     4141 tcttcaccat cgtcgcctac ctggcgatac gcacccggga gcgggccgac ctcggccgtg
     4201 tcgtcgttgg gctctgggtc ggcttgctca ccctggccgg ggtattgctc atcctcatcg
     4261 tcgggacgct cttcggccag accgtcctcc tcggcgacgg tgtcgtcggt ctcctcctgt
     4321 tggtgccagc tgcgaatacc atcctggctt ggatcttcgc acccatggtg gtccggctta
     4381 tcgaccgtga cacgaccgcc ttccgatacg catgaaattc tcagttcgcc tttcgattct
     4441 cggcatggca ttcatcgcca tgttctcgat aatcggccta cgactgtggt tcgtccaggt
     4501 cgcacagggc ccggccatcg cccaggccgc cgaggagcaa acctggctca gcaagaccac
     4561 acatgcgccg cgaggcgaca tcttcgaccg gaacaaccat ctcctcgtca ccagccgcct
     4621 cactccggcg gtcttcatcg atcgaacctt cgtccagccg gacgaccaga acatcctgat
     4681 tcagcgtctg tcggctctgc tggcgatgga cccggccgac ctcacccacc tgtacgacga
     4741 agccggcgtg aacggtcgtt ttgaggtggc caccgtcgac aacgaggtgg cgaatcagat
     4801 aagcgagcag ttgggaagcc tccccggagt ggagatcgtg aacgtccccg agcgggtcta
     4861 cctgtcgggt gacacactcg ctcacgtgat cggccatctc ggtctgccgg accaagccga
     4921 cctcgacgcc cgcgaggacc tcgatccgac cgcccgcatc gggaaactag gagtcgagag
     4981 gtcctatgac gatgtcttgc agggcgagtc tggctctgtc gaatacagag tcaggctggg
     5041 tgagatcatc gagcagaggc cggccatcga tccgatcccg ggcaactcac tgatcctgac
     5101 caccgactcc gatctccagc aagttgtcga gctcgcactc gaagagggga ttcgtctctc
     5161 caacgaggtg aaggcggctg cgcccccgga cgaggagttc ttcaccgaga ccaaacgggc
     5221 ggcggccgtc gtcctcgacc cacagacctt cgatgtgctc gcccttgcct cagtgcccga
     5281 cttcgacccg gcgctgttcg tttcgggaat cgagacggag aagttcgacg agctgaacga
     5341 gtctcttgcc ttcaacaacc tggcggtgag cggtcaattc gccccggcct cgaccttcaa
     5401 ggcgatcact tacaccgcct tcgtcgagga ggacctgcct ctgcccgagg acaccgactt
     5461 catcgatcct tcggccaagg aagtcaactg tgacggggag tgggtcttca cgctccagga
     5521 cggctcgccg cagcggaagt tcgactggta ctacccgcga acgctcggct ggctcgatct
     5581 ccacggcgca ttggagaatt cctgtaacaa gttcttctgg tcgattggtc tcggtgcctg
     5641 gcagaaccgg gccacggtgg gagagacggt tctccaggac tgggcgatgg acctcggtta
     5701 cggctcggag accggcatcg acctgggcag tgaggcagcc ggcatcgtgc ccacaccggc
     5761 cctgttcgag gagtgggccg agtatcagag ggagaacccc gacgagccgc cccgactcga
     5821 cgccgaccga ttggatccga cactggtcag cccattcttc ggtggcgacc tcatggactt
     5881 cgcgataggc cagggagcgt tcaccgccac cccgctccag gccgcggtgt cgtatgcgat
     5941 cctggccaac ggtggccggg tcatggagcc gagagtcgtc gacaagatca tctctccaga
     6001 cggggagctc atcgaggacg tttcctccga ggtggtacgg accgtgccga tctcagacac
     6061 gacgcgtcaa caactgctgg cagacctcaa caaggtggtg accaccggca ccgcagccgc
     6121 ggccttcgag tcattcggcc ccggcctcga actggtggcg ggcaagaccg gaaccaccca
     6181 ggtctcccag accagagacg accatgcctg gttcatcggc ttggccccca tcgacgaccc
     6241 tcaattcgtg gtggcggtgg tggtcgaaga gggcggctcc ggcggggcaa tcgcggcccc
     6301 tgtggcccgt cacatcctcc aatacctgat gggcaatgag ccgaccgaga tcatcgcagg
     6361 agatcaaaca gactgatgac tgctgtactc actcgcatcc gtgaagtggc cgtggtccgg
     6421 cgcaacaacc ggaccgattg gatcttggtc ggtacggtgt tgcttctctc gggcttcggc
     6481 ctgttgatga tctactcggc cacccgcagc gtgggcacct tctcgatgga gcggcagatg
     6541 atcttcgttg ctgccggcct gatcatcttc gccatcgtgt cgaacatcga ctatcgggag
     6601 taccggagca tcgtccccgc ggcgtcgatc atcatcctcc tgcttctcgc cgctgtcttc
     6661 ttctttccgg agatcaatgg ggcgagaagg tggatcccta ttggattctt cgctctgcaa
     6721 ccggccgagt tcgccaagat cgttgtcata gtggccctgg cctcattgct cgccccaccg
     6781 gcatcgcggg aagacctgat gagcgggccc cgtcagctca gctgggcgaa agtcggccag
     6841 gcgctactgg tcgtggctgt gcccggggcc ctcatctacc tcgagcccga tctgggaacc
     6901 acactcgtct tcgggttcat cgtgttcggc atgctgttcg tcgccggggc ctcctggcgc
     6961 cagctcaccg ccctggcgac cggcgtcggc gtcgccggac tgctgatctg gcgcctggac
     7021 ctcctccagc cccatcagct caaccgcctc gaggtcctct ggaacccgga tatcgatcca
     7081 caagggatcg gctatcagtt gcgcca
//
LOCUS       DASUTA010000311         2136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1647977, whole genome shotgun sequence.
ACCESSION   DASUTA010000311 DASUTA010000000
VERSION     DASUTA010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1647977"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VFZ80_06190"
     CDS             <1..747
                     /locus_tag="VFZ80_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007458184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6287055.1"
                     /translation="FRLLRPDSGSVTWNGEPIKPEDLLRFGYMPEQRGLYPKMRAGDH
                     LAYLGRLHGMSKPAARESAELWLERFGLGERVGDPVEALSHGNQQRVQLAAALVHDPE
                     LLVLDEPFSGLDPIAVETMSDVLREQAAAGKAVVFSSHQLDLVEDLCEEVAIIDDGHV
                     VVRGNVERLKDSAPIRHLELETEGDPSELLGFLDGVQSTVMDGERLTAIIAAETDVRG
                     FIAHAQQAGKVRHFAYKTPSLSDLFREAVR"
     gene            744..1958
                     /locus_tag="VFZ80_06195"
     CDS             744..1958
                     /locus_tag="VFZ80_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6287056.1"
                     /translation="MTGFATLWQVALREISERVRSKAYLITTGLTVLIVLGLIVAPSI
                     FGSETTESTVGSVGAGNDVIISTAVDLGNANDEPGDPPSVAIETVEFEDRESAETALD
                     DGEVDAVIIDGEEVIVQSVGGFTGNSLLSGLQRAAGTVQIQEIVEEEGQQAADIIEIL
                     TSDPLETTTLTEGDPEDGDSRTLIAYFGLLLLYLAVLLYGTWILTGVTEEKSNRVVEV
                     LLSSVRPWQILGGKILGIGSLALLQLLGTIVVALVALRVTGAFDLPPIEIEAVLNLLL
                     WFVVGFVIFAVLFGAAGSLVSRTEEANTIAMPMSMAAVVGFFVSISALNDPDGVVATI
                     FTFVPITAPFVVPVRAALQSIPAWQYILALVICLVSIGVLTVLAGRIYAGGLLRYGGR
                     VGVREAWQSAAE"
BASE COUNT          369 a          693 c          678 g          396 t
ORIGIN      
        1 ttccgcctcc tccgccccga ctcggggtcg gtgacgtgga atggcgagcc gatcaagccc
       61 gaggacctcc tccgctttgg ctacatgccc gaacagcgcg ggctctaccc gaagatgagg
      121 gccggcgacc atctcgccta tctcgggcgt ctccacggga tgtcgaagcc ggccgcacgg
      181 gagtcggcgg agctttggct ggaacggttc gggctcggcg agagagtagg tgatcctgtc
      241 gaggcgttgt cccacggcaa ccagcaacga gtccagttgg ccgcggcact ggtccacgac
      301 ccagagctcc tcgtcctcga cgagcccttc agcgggctcg acccgatcgc ggtcgagacg
      361 atgtccgacg tcctccgcga gcaggccgcc gccgggaagg ccgtcgtgtt cagctcccat
      421 caactcgacc tcgtcgagga cctgtgcgag gaggtggcga tcatcgacga cggtcacgtc
      481 gtggtcaggg gcaacgtcga gcggctcaag gactccgctc ccatccgcca cctcgagctg
      541 gagaccgagg gtgatccgtc ggagttgctc ggattcctcg acggggtcca atcgacggtg
      601 atggacgggg agcgtctcac cgccatcatc gccgcagaga ccgacgtcag aggtttcatc
      661 gcccacgccc agcaggcggg caaagtgaga cacttcgcct acaagacgcc ctcgttgtct
      721 gacctcttcc gagaagcggt gcgatgaccg gtttcgcgac gctctggcag gtggcactcc
      781 gagagatctc cgagcgagtt cgctcgaagg cctacctgat caccaccggg ctcaccgtgc
      841 tcatcgtcct cggcctcatc gtcgctccga gcattttcgg ctcggaaacg acggagagca
      901 cggtcggctc ggtcggtgca ggcaacgacg tcatcatctc caccgccgtc gacttgggca
      961 acgccaatga cgagcccggg gacccgccga gtgtcgccat cgagacggtc gagttcgaag
     1021 accgggagag tgcagagacg gcactcgacg atggggaggt cgacgcagtc atcatcgatg
     1081 gggaggaggt catcgtccag agcgtcggcg ggttcaccgg caactctctt ctcagtggcc
     1141 tgcagcgggc tgccggaacc gtccagatcc aggagatcgt cgaggaagaa ggccagcagg
     1201 ccgcggacat catcgagata ctcacatccg acccgctcga gacgacgacc ctgaccgaag
     1261 gtgatccgga ggatggcgac agtcgcacat tgatcgctta cttcggtctg ttactcctct
     1321 acctggcggt cctcctgtac gggacatgga ttctcaccgg cgtcaccgag gagaagtcga
     1381 accgtgtcgt cgaggtgttg ctctcgagtg tccggccatg gcagatcctt ggtgggaaga
     1441 tcctcggaat cggctccctg gccctgctcc agcttctcgg caccatcgtg gttgctctgg
     1501 tggcgttacg ggtcaccggc gcattcgacc tgccgcccat cgagatcgag gccgttctca
     1561 acctcctctt gtggttcgta gtgggattcg tgatcttcgc ggtcctattc ggtgccgccg
     1621 ggtcgctggt gagccgaacc gaggaggcaa acaccatcgc catgccaatg tcgatggcgg
     1681 cggtcgtcgg cttcttcgtc tcgatcagcg ccctgaacga ccccgatggc gtggtggcca
     1741 cgatcttcac gttcgtgccg atcaccgctc ccttcgtcgt gccggttcgg gccgccctgc
     1801 agtcgatccc ggcctggcag tacatcctgg ccctggtcat ctgcctggtc tcgataggtg
     1861 tcctcaccgt tctcgccgga cggatctacg ccggtggcct cctccgctac ggcggacgag
     1921 tcggagtcag ggaggcttgg cagtcggcag ccgaatgaga ttcggcgtcg aatagcctgc
     1981 ctgcgatgcc ctatgaacac ctggagttgg agaagaccgg ccacgtagcc acgctgtggg
     2041 tcaaccgccc cgacaaactg aacgccctat ccgaagacat gtgggccgac atcccggcgg
     2101 ctgtctccga gctcgatcat gacgatgacg ttcgcg
//
LOCUS       DASUTA010000312         6321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1648046, whole genome shotgun sequence.
ACCESSION   DASUTA010000312 DASUTA010000000
VERSION     DASUTA010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648046"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..995
                     /locus_tag="VFZ80_06200"
     CDS             66..995
                     /locus_tag="VFZ80_06200"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6287057.1"
                     /translation="MPTNLASGAEYDTVGQRRWVWPAFAIPGVVWLSLLFLVPFYAIF
                     AVAMGTIDPILSRPVPEWNPIYWNPTIFAEVLGELFGGQLGSTALRTIGYVFVASALC
                     LLVGYPVAYFVSRKAGRFRWLWLLLILAPFWINYLMRMMSWVNLLSPDGQINQVLSVF
                     GVESFPDWLSGHHFVVILGLVYGYIPFFILPLFAALDRIDDATLEASRDLGANNWQTF
                     WRVTWPLSRNGVLGGLVIIMLPMFGDYYTPNLLSGSPRTRMLGNTIDQFINQTTSQGA
                     VGAALTIVLMAFVAGLMIYYLVTINRATEEARR"
     gene            992..1861
                     /locus_tag="VFZ80_06205"
     CDS             992..1861
                     /locus_tag="VFZ80_06205"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6287058.1"
                     /translation="MRNQWGKPRGLVLLTALYVLWSLIPVAIAVRFSFNAGRSRSTWQ
                     GFSTMWYVGDPADASSVLYDPSLRFALNQSIKLALLTMLVATPIGVALAIGLARWRGR
                     GSKSSNFLMLFPLVTPEIVMGVALFLVFDNLYDFVTFGTMAQFLGHVTFSISFVVIIV
                     RGRLFAIGRDYEEAAMDLGASPSEAMRRVLLPLLTPALIASFAIVFALSIDDFVISQF
                     LVGGADSVTIPVRLYSGARLAPPPSLNALATVLMVITIGAIVIAGLGFKYLGRRFGGG
                     EDSALETVGRFEI"
     gene            complement(1858..2250)
                     /locus_tag="VFZ80_06210"
     CDS             complement(1858..2250)
                     /locus_tag="VFZ80_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006345749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6287059.1"
                     /translation="MPRITTTNVHVDDQGQALDFYTNVLGFVVKDDIDLGEARWLTVV
                     SADEPNGTELLLEPSDHPAVEPYRSALVADGIPAAMFAVTDVEVEYERLRDLGVHFTQ
                     KPMNAGPVTTAVLDDTCGNLIQLVSPNG"
     gene            complement(2257..2529)
                     /locus_tag="VFZ80_06215"
     CDS             complement(2257..2529)
                     /locus_tag="VFZ80_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6287060.1"
                     /translation="MGDVFKALADPTRRKILDELVEEEPQTLFEICARLTMKHGIGST
                     RQAISQHLDVLEDAGLVRTERSGRYKLHHLDTTALDQITTRWPRKE"
     gene            complement(2658..3755)
                     /locus_tag="VFZ80_06220"
     CDS             complement(2658..3755)
                     /locus_tag="VFZ80_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018461718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6287061.1"
                     /translation="MSGDVELVGLVKTFGEVTAVDNIDLHVEDGEFFSLLGPSGCGKT
                     TTLRLIAGFERPDAGAILIDGVDMATTPPHKRPVNTVFQSYALFPHLSVEENVGFGLK
                     YQEMSKAEARVKIGRALELVRLGGMEKRRPNQLSGGQQQRVALARSLVLNPSVLLLDE
                     PLGALDAKLRKALQIELKTLQEEIGITFMYVTHDQEEALTMSDRMAVMSHGRVEQVGT
                     PSDVYELPVSSYIADFLGVSNLMEVMVKSQNGDGSAIVQLGSHELTTSSSYPWPGPAM
                     VSIRPERIKIVGDGSTLPNQVPATVDRLVYAGPVLNVLVQIEDLNEITVALPNEGHLT
                     RYQHGETVNLHLPAEAIRVLDRAEPEELPAS"
     gene            complement(3777..5144)
                     /locus_tag="VFZ80_06225"
     CDS             complement(3777..5144)
                     /locus_tag="VFZ80_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX6287062.1"
                     /translation="MLSPVDQASTVLDELIQEEEATFVERSPKAAEYAARANRILPGG
                     VTSSWQISRPRPIWIEEGSGSRVRDVDGNEFIDYHGGYGAMLAGHAHPAIVAAVTERV
                     RKGTHFAQPTPDALAVTEELVERYGQPLWRFGNSGTEATMDAIHLMRAVTGRDLILKF
                     EGAYHGHHDAAMVSVWNEDGLGPEHRPVAVAAGGGIPQAMVDLTLVATFNDLELVERL
                     FEEHPGQIAGVITEPVLQNCGVLLPGDGFLQGLRDITRRHDALLTFDEVKTGLTLGRG
                     GATRRFGVQPDIVCLAKALGGGLPISAIGGIEEVMGAITDGTYEQVGTFNGNPLSMAA
                     AKAMLTEVMVPETYQHLETLQERMREGTEEIIDRHELVAHVATAGAKGSVIYSAEPTR
                     TYRDFLRVDGRYAHAAWLYQFNRGVFLPPWAKGEQWLISVQHTVEDIERYLETLDGFA
                     SVISS"
     gene            5225..>6321
                     /locus_tag="VFZ80_06230"
     CDS             5225..>6321
                     /locus_tag="VFZ80_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX6287063.1"
                     /translation="MSVAPLSQIEIARVVAPFGQNSTLPADAYISEEVFAWEMDNIFK
                     ATWLCVGRGEELVAPGQIRAIDVAGEGVLLARDREEGALTAFSNVCRHRGHELAPVGD
                     PIDARLIRCPYHSWSYRFNGELRAAPTFTQTPGFDMSDYPLIGIAVAEYLGWVFVDLS
                     GEAPPIEQFFGNLADFAKPYETDRIRTAAVHDYVVDANWKIIVENYNECYHCSSIHPE
                     LCEVTPPDSGLDREPTGIWCGGTMILKDHAVTMSLDGSSKGVNFAGLDAEAARDVWYL
                     TVMPNLLLSMHPDYVMTHRLTPLSTGQTHIECSWLFPPEAFELEGFDPAYAVDFWDIT
                     NREDWTACENVMKGVRYQGYRPGPLSNWEGTV"
BASE COUNT         1037 a         1975 c         2002 g         1307 t
ORIGIN      
        1 cgagaccgag gccctgtggt cggatcagtg gaccagagcc accagcggat gaagtgagct
       61 agatcttgcc cacaaacctg gcctccggcg ccgaatacga caccgtcggc cagaggcggt
      121 gggtctggcc ggccttcgcc atccccggcg tggtctggct atctcttctc ttcctggtcc
      181 cgttctacgc catcttcgcg gtggcgatgg ggacgatcga ccccatcctg agccgaccgg
      241 tccccgagtg gaatccgatc tactggaacc cgaccatctt cgccgaagtg ctcggcgagc
      301 tgttcggcgg tcagctgggg agcactgctc tgcgcaccat cggctatgtc ttcgtggcct
      361 cggcgttgtg tctgctcgtc ggttatccgg tggcctactt cgtctcccgc aaggcgggcc
      421 gttttcgctg gttgtggctg ctgctgatcc tcgccccttt ctggatcaac tatctgatgc
      481 ggatgatgtc gtgggtaaac ctgctcagcc ccgacggcca gatcaaccag gtcctgagcg
      541 tgttcggcgt ggagagcttt cccgattggc tctcgggcca tcacttcgtc gtgatcttgg
      601 gcctggtgta cgggtacatc cccttcttca tcctgccctt gttcgccgca ctcgaccgca
      661 tcgacgacgc caccctcgag gcgagccgcg atctcggcgc caacaactgg cagacgttct
      721 ggcgggtgac atggcccctg tcccgtaatg gggtcctggg cgggctggtg atcatcatgc
      781 tccccatgtt cggcgactac tacacgccga acctgctctc tggctcgcca cgaacccgga
      841 tgctgggcaa cacgatcgac cagttcatca atcagaccac gagccagggt gcagtggggg
      901 cggcgctgac catcgtcctc atggcgttcg tcgccggtct gatgatctac tacctggtga
      961 ccatcaaccg agccaccgag gaggcccgca ggtgaggaac cagtggggca agccgcgcgg
     1021 gctggtgctg ttgacggcgc tatatgtgct gtggtcgctg attccggtgg cgatcgcggt
     1081 gaggttctct ttcaatgccg gtcgatcgcg gtcgacatgg cagggctttt cgacgatgtg
     1141 gtacgtgggc gaccccgccg atgcgtcctc ggtcctctac gacccgtcgt tgcgcttcgc
     1201 cctgaaccag agcatcaagc tcgccctgct cacgatgctc gtggcaaccc cgatcggtgt
     1261 cgccctggcc atcggtctgg cccggtggcg gggaaggggc tcgaagtcgt cgaacttctt
     1321 gatgctcttc ccactggtga ctcccgagat cgtgatgggt gtcgccctct tcttggtgtt
     1381 cgacaacctc tacgacttcg tcaccttcgg cacgatggcg cagttcctgg gtcacgtcac
     1441 gttctcgatc tcgttcgtgg tgatcatcgt gagaggaagg ttgttcgcca tcggcaggga
     1501 ttacgaggag gcggctatgg acctcggtgc ctccccatcc gaggcaatgc gccgcgtcct
     1561 cctgccgctg ttgaccccgg cgttgatcgc ctcctttgcc atcgtgttcg ccctctccat
     1621 cgacgatttc gtgatcagcc agttcctggt gggcggcgcc gactcggtga cgatccccgt
     1681 ccgtctctac tcgggagcac gattggcacc gcccccgtca ttgaacgcac tggcgacggt
     1741 gctcatggtg atcaccatcg gggccatcgt catcgctggt ctcgggttca agtacttggg
     1801 ccgccgcttc ggcggtggtg aggattccgc cctggagacg gtgggccgat tcgagatcta
     1861 gccattgggg ctgaccaact gaatgaggtt gccgcaggtg tcgtcgagga cggccgtggt
     1921 caccggacct gcattcattg gcttctgcgt gaagtggaca ccgaggtccc tgagccgctc
     1981 gtactcgacc tccacatcgg tgacggcgaa catcgctgcc ggaatgccgt cggcgacgag
     2041 tgccgaccgg tatggctcca cagccgggtg gtccgacggc tcgagaagaa gttcggtgcc
     2101 attgggctcg tcggctgaca cgacggtgag ccatctcgcc tctccgaggt cgatgtcgtc
     2161 cttcacgacg aagccgagca cgttggtgta gaagtcgaga gcctggcctt ggtcgtcgac
     2221 atggacgttc gtagtggtga tcctgggcat cgctggtcac tcctttcgtg gccatctcgt
     2281 ggtgatttgg tccagtgcgg tggtgtcgag gtgatggagc ttgtatcggc cgcttcgctc
     2341 ggttctgacc aagccggcgt cctccaatac atcgaggtgt tgagatatcg cctgccgggt
     2401 agatccgatg ccgtgcttca tggtcaaacg agcacatatc tcgaatagtg tctgtggctc
     2461 ctcttcgacc aactcgtcga ggatcttgcg acgtgtcggg tcggcaagcg ccttgaagac
     2521 atcacccacc cgccgagcat atgcaagcaa tcacttgcct gtcaacccgc cgagaggctc
     2581 acggagatga ctctcgtttc cgtcttcggc cctcccttgg agggcaccga gcagactgaa
     2641 cggacacggc gatgggccta cgacgcgggc agctcctctg gctcggcccg gtcgagcacc
     2701 cggatcgcct cggcaggcag atgcaggttg accgtctctc cgtgctggta acgggtcaga
     2761 tgcccttcgt tgggaagcgc aacggtgatc tcgttgagat cctcgatctg gaccagcacg
     2821 ttgagcaccg ggccggcgta gacgagacgg tcgacggttg ccggcacctg gttggggagc
     2881 gtcgagccgt ctccgacgat cttgatccgc tccggtcgga tcgagaccat cgccggccct
     2941 ggccacggat aggaagacga cgtggtcagc tcatgggagc cgagctggac gatggccgat
     3001 ccgtcaccgt tctgggattt gaccatcact tccatgaggt tggagacacc gaggaaatcg
     3061 gcgatgtagc tgctcaccgg cagttcgtat acatccgacg gggtgcccac ctgttccact
     3121 ctgccgtgtg acatcactgc catccggtcg gacatcgtca gcgcctcttc ctgatcgtgg
     3181 gtcacgtaca tgaaggtgat gccgatctct tcctgaaggg tcttcagctc gatctggagc
     3241 gccttgcgca gcttggcgtc gagagcgccg aggggctcgt ccaacagaag caccgagggg
     3301 ttgagcacca atgagcgtgc cagggcgacc cgttgttgct ggccgcccga cagctgattg
     3361 ggtctgcgct tctccattcc gccgagtcgc accagctcca gggctcggcc aatcttcact
     3421 ctcgcctcgg ccttgctcat ctcctgatac ttgagaccga acccgacgtt ctcctcgacg
     3481 ctcaggtgag ggaacagtgc gtaactctgg aagacggtgt tgacgggacg tttgtgcgga
     3541 ggggtggtgg ccatgtcgac gccatcgatg aggatggcac ctgcgtcggg acgctcgaac
     3601 ccggcgatga gtcgcagcgt cgtcgttttg ccgcatcccg aagggccgag cagggagaag
     3661 aactcaccgt cctcgacgtg caggtcgatg ttgtcgacgg cggtgacttc tccgaacgtc
     3721 ttgaccagcc cgaccagctc tacgtcacca ctcatggcga cggcgagtgt atggcttcag
     3781 gatgagatga cactcgcaaa accgtccagt gtctccagat atctctcgat gtcctcgacg
     3841 gtgtgctgga ccgagatcag ccattgctcg ccttttgccc acgggggcag gaagacgccc
     3901 cggttgaact ggtagagcca ggcggcgtgg gcatagcggc cgtccacccg gaggaagtcg
     3961 cgataggtcc gcgtcggctc ggctgagtag atgacacttc ccttggcgcc ggctgtggcc
     4021 acatgggcga ccagctcgtg gcggtcgatg atctcctcgg tgccttcgcg catccgctct
     4081 tgcagtgtct ccaggtgctg gtacgtctca ggcaccatca cctcggtcag catcgccttc
     4141 gccgcggcca tgctgagcgg gttgccattg aacgtgccga cctgctcgta tgtcccgtcg
     4201 gtgatggcgc ccatcacctc ttcgatcccg ccgatcgcag agatggggag acctccgccc
     4261 agggccttgg cgagacacac gatgtcgggt tgaactccga aacgcctcgt ggcgccgcct
     4321 ctgccgagcg tgagcccggt cttgacctcg tcgaaagtga gcagggcgtc gtgacgacgg
     4381 gtgatgtcac gcagcccctg gaggaaaccg tcgccgggga ggagcacgcc gcagttctgc
     4441 aggacgggct cggttatgac tccggcaatc tggcccgggt gctcctcgaa gagcctttcc
     4501 acgagctcga ggtcgttgaa agtggccacc agcgtgagat cgaccatcgc ctgagggatg
     4561 ccgcctccgg cggcgacggc gacggggcgg tgctccggac cgagaccgtc ttcattccag
     4621 accgagacca tggctgcgtc gtggtgaccg tgataggcgc cttcgaactt gaggatgagg
     4681 tcgcgcccgg tgaccgcccg catcaggtgg atggcgtcca tggtcgcctc ggtgccggag
     4741 ttgccaaagc gccagagggg ctggccgtag cgctcgacca actcctcggt cactgccaag
     4801 gcgtcgggtg tgggttgggc gaagtgggtt cctttgcgaa ccctctcggt caccgcggcc
     4861 acgatggcgg gatgggcgtg gccggcgagc atcgctccgt atccgccgtg gtaatcgatg
     4921 aactcgttcc cgtcgacatc cctgacgcgg gacccgctgc cttcctcgat ccaaatcggc
     4981 ctgggacgtg agatctgcca gctggaggtg accccgccgg ggagtatccg gttggctcga
     5041 gccgcgtatt cggcggcttt gggggagcgc tcgacgaatg tggcctcttc ttcctggatc
     5101 agttcgtcga gcactgtcga cgcctgatcg accggggaga gcatgatgac ctttctgtct
     5161 gtagagtatc ctgaaccgcc attcaggata caccggcgtc gtctaccctg tcggagataa
     5221 aacgatgagc gtcgcacctc tcagccaaat cgagatcgca cgggtggtgg cacccttcgg
     5281 ccagaactcg accttgcccg cggatgcata catctcagag gaggtcttcg cttgggagat
     5341 ggacaacatc ttcaaggcga cctggctgtg cgtcggccgg ggtgaggagc tggtcgctcc
     5401 cggtcagatc agggcgatcg atgtcgcagg tgagggtgtg cttctcgccc gtgatagaga
     5461 ggagggggcc ctcaccgcgt tctccaacgt ctgccggcac cgcggtcacg agctggcgcc
     5521 tgtcggcgac cccatcgatg cccggctcat ccgctgtcca tatcactcct ggtcgtatcg
     5581 cttcaatggc gagctcaggg ctgcgccgac attcacacaa acccccggtt tcgacatgag
     5641 tgactacccc ctcatcggga tcgcggtggc cgagtatctc ggttgggttt tcgtcgatct
     5701 cagcggcgag gcgcccccca tcgagcagtt cttcggcaat ctggcggact tcgccaagcc
     5761 ttacgagacc gatcggatcc ggactgccgc cgttcacgac tacgtggtcg acgccaactg
     5821 gaagatcatc gtcgagaact acaacgagtg ctaccactgc tcgtccatcc atcccgagct
     5881 gtgcgaggtc acgccaccgg acagtggtct cgatcgggag ccaaccggca tctggtgcgg
     5941 ggggacgatg atcctcaagg accacgcagt gaccatgtcg ctcgatggca gctcgaaggg
     6001 tgtcaacttc gccggtctcg acgcagaggc ggcccgggac gtctggtact tgacggtgat
     6061 gccgaacctg ctgctctcca tgcaccccga ttacgtgatg actcaccggt tgaccccgct
     6121 gtcgacgggt cagacccaca tcgagtgctc ctggctgttc ccgccggagg cgttcgagct
     6181 cgagggcttc gatcccgcct acgccgtcga cttctgggac atcaccaacc gtgaggactg
     6241 gacggcctgt gagaacgtga tgaagggtgt tcgctaccag ggataccgcc ccgggcccct
     6301 ctccaactgg gagggcacgg t
//
LOCUS       DASUTA010000313         4284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1648456, whole genome shotgun sequence.
ACCESSION   DASUTA010000313 DASUTA010000000
VERSION     DASUTA010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648456"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFZ80_06235"
     CDS             complement(<1..330)
                     /locus_tag="VFZ80_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6287064.1"
                     /translation="MRLLVVEDEPDLADVIARGLRRDGHAVDVAHRSGEADLKLRSAG
                     YDVVLLDWNLPDGSGLQLCRDLVSGELPTLEGDRPKILMLTARDDVEDRVAGLDSGAD
                     DYLVKPFA"
     gene            467..637
                     /locus_tag="VFZ80_06240"
     CDS             467..637
                     /locus_tag="VFZ80_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287065.1"
                     /translation="MTFITEDRDGFHTYTGHVVSRGPSTIVVLLTEPFSLAGRSVQFP
                     IDNVVSEKVISS"
     gene            671..940
                     /locus_tag="VFZ80_06245"
     CDS             671..940
                     /locus_tag="VFZ80_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287066.1"
                     /translation="MFLEASTLQALAIVGGVGAIAGLVAGFLASADNLVGTVLMGIIG
                     GIALSAIFRIAGFPAIYSVGEEEFSLVWAAVGGLVLGFVVGRSNA"
     gene            1114..1683
                     /locus_tag="VFZ80_06250"
     CDS             1114..1683
                     /locus_tag="VFZ80_06250"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX6287067.1"
                     /translation="MPSFEALIRDVDAERLAHSYTPDCPVSPSEVVLISLNHWGFDGE
                     VHEGSIVVARDEGDRVVAIFGRLFDIGYPIESVIPIGDLPEGIEDDDPDYNNTSGLHC
                     RRATGSTRWSEHSRGLAIDINPLLNPFITPNILWPKNSAKYLDRTLGEPGMIVAGDEV
                     VRAFEDNGWLWGGYWTSIKDYQHFSVSGR"
     gene            complement(1721..3115)
                     /gene="sufB"
                     /locus_tag="VFZ80_06255"
     CDS             complement(1721..3115)
                     /gene="sufB"
                     /locus_tag="VFZ80_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX6287068.1"
                     /translation="MDLGGYQLGWRDEVELAFEPRKGLDEDVIRDMSAMKREPEWMLD
                     FRLKAYKRFLRKPIPQWGGGGALNTIDFDDIYYYVKPEGEQQKDWDMVPESIKETYEK
                     LGIPEAERKYLAGVTAQYESEVVYHRNREDLEKLGVLFTDMDTAVREYPEIVKEHFGT
                     VIPPNDNKFAALNSAVWSGGSFIYVPPGVHVDQPLQAYFRINSENMGQFERTLIIVDD
                     GAYVHYVEGCSAPTYTTDSLHSAVVEIIVKEGGRCRYTTIQNWSNNVFNLVTKRAAAY
                     RNATMEWVDGNLGSRLTMKYPAVWLMEPGAHGEVLSIAFAGEGQHQDAGAKMVHAAPD
                     TTSTIVSKSISKGGGRSGYRGLVRVEPGAENAKSFVRCDALILDEDSRSDTYPYMEIE
                     ENDAEIGHEATVSKVGEDQLFYLMSRGLTEDQATSMIVAGFIEPIIKELPMEYAVEMN
                     RLIELNMAAAGAIG"
     gene            3285..3725
                     /locus_tag="VFZ80_06260"
     CDS             3285..3725
                     /locus_tag="VFZ80_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287069.1"
                     /translation="MPMIVVGADTTPGAVLLSQLDASAREIRVFVTDEARGLELKGAG
                     FKVATGDVSDDSHVEAAATRCFSAVLISEAAHDDRERSFARNAADVLEGWAKAVSNSK
                     VRRVIWVTDGDYPDTSVPEVAVVDPSETDFARKIVDLDEAAKLS"
     gene            complement(3738..>4284)
                     /locus_tag="VFZ80_06265"
     CDS             complement(3738..>4284)
                     /locus_tag="VFZ80_06265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6287070.1"
                     /translation="PARAQSDPPFVVVFPQETSKTYFSSSYGDYRSGGRYHKGNDLMA
                     PRLTEVYAMADGVVFHVGINRLSGRNVKIDHGGGWESYYLHLNNDNPGTDDGDAPWSL
                     TVAPGIEEGTAVEAGQLIGWVGDSGNAERTAPHTHFEIRHDGSAIDPYQILIDAYERE
                     VAAEQYLAMMLEADRSVYPVE"
BASE COUNT          784 a         1332 c         1329 g          839 t
ORIGIN      
        1 cgcgaacggc ttcaccagat agtcgtcggc tccggagtcg agaccggcga cgcggtcttc
       61 tacgtcgtcg cgcgcggtga gcatgaggat cttgggccgg tcaccctcaa gcgtcggcaa
      121 ctcacccgag accaggtcac ggcacagctg gagccctgac ccgtcgggga ggttccagtc
      181 gagcaaaacc acgtcgtatc cagcagagcg cagcttgaga tcggcctcac ccgacctgtg
      241 ggcaacgtcg acggcatgcc catcgcggcg cagaccccgg gcgatgacgt cagcgaggtc
      301 gggctcgtct tcgacgacta ggagacgcat cgtgtctcaa gataagcggg tgcacctaat
      361 cggcgaataa acacggattc ggatcgcacg gcgcgagtat ggacttccga cccatatgga
      421 aacggcaacc ttttgccgag tctggtagtc ggagtagcaa atgatcgtga ctttcatcac
      481 ggaagaccgg gacgggttcc acacatatac aggccatgtc gtcagccgag ggccgtcgac
      541 cattgtcgtc ctgctgaccg agccgttcag cctcgcgggt cgttcggtgc agtttccgat
      601 cgacaacgtc gtgtcagaaa aggtgatctc gagctaggga agcgccctgg atccggaata
      661 ggctgacatc atgtttctcg aggcttcgac tctccaggct ctcgccatcg tcggaggcgt
      721 gggcgccatc gccgggctcg tggccggatt cctcgcatca gcagataatc tcgtcggcac
      781 cgtgctcatg gggatcatcg gagggatcgc cttatcagcc atatttcgca tcgccgggtt
      841 tcccgccatc tactcggtcg gcgaggagga gttctccctg gtgtgggcgg cggtgggcgg
      901 tctcgtcctc ggattcgtgg ttggtcgctc caacgcctga cctcactgct tctagctgcc
      961 tccttgctgg tggcgtgcgc cgggcctgag cccgctgcac cgaccacgga ttcgatctcc
     1021 accacagcgc cagtcatccg tacgacgagc accagctcga ccacgaccac atcatcgacc
     1081 acgacgacct cgccacccac caccacgacc acggtgccgt cgttcgaggc tctcatccgg
     1141 gatgtcgatg ccgagcggct ggctcacagc tacacaccag attgtccggt gagccccagc
     1201 gaagtcgtgc tgatctccct gaaccattgg ggattcgacg gcgaggttca cgagggctcc
     1261 atcgtggtgg cccgagatga gggcgatcgc gttgttgcca tcttcggtcg gctcttcgac
     1321 atcggctatc cgatcgagag cgtgatcccg atcggagatc tccccgaggg catcgaggac
     1381 gatgaccccg actacaacaa cacatcgggg ctgcattgcc gccgcgccac cggatcgacc
     1441 cgatggtccg agcattcgcg aggcctggcc atcgacatca accctctcct caaccccttc
     1501 atcactccca acatcctctg gccgaagaac tcggcgaagt acctggaccg aacgctcggg
     1561 gaaccgggga tgatcgtcgc cggcgacgag gtggtgcggg cgttcgagga caacggctgg
     1621 ctctggggcg gctactggac ctcgatcaag gactaccaac acttctcggt cagcggccgg
     1681 tgaccccaca ttgcgaaccc cgggttcggg aaagcgggtg ctagccgatg gcgccggcgg
     1741 cggccatgtt gagctcgatg aggcgattca tctcgactgc atactccatc gggagctctt
     1801 tgatgatcgg ctcgatgaag ccggccacga tcatcgaggt ggcctggtcc tcggtgagac
     1861 cgcgtgacat cagatagaag agctggtcct cgccgacctt ggagacggtc gcctcgtggc
     1921 cgatctcggc atcgttctcc tcgatctcca tgtaggggta ggtgtcggag cggctgtcct
     1981 cgtcgaggat gagcgcgtcg cagcggacga agctcttggc attctccgca cccggctcga
     2041 cccggaccag accgcgatag cccgaccgcc cgccaccctt cgagatcgac ttggagacga
     2101 tcgtcgaggt ggtgtccgga gcggcgtgga ccatcttggc cccggcgtcc tggtgctgtc
     2161 cctcaccagc gaatgcgatg gagaggacct cgccatgggc gcctggctcc atgagccaaa
     2221 cggccgggta cttcatggtc agtcgcgagc cgaggttgcc gtcgacccac tccatggtgg
     2281 cgtttcgata ggccgcggcg cgtttggtca ccaggttgaa cacgttgttc gaccagttct
     2341 ggatggtggt gtagcggcaa cgcccgcctt ccttgacgat gatctcgacc accgccgagt
     2401 gaagggaatc tgtggtgtac gtcggtgccg agcacccctc gacgtagtga acgtaggcac
     2461 cgtcgtcgac gatgatcaag gtccgctcga actgacccat gttctccgag ttgattcgga
     2521 agtaagcctg gagtggctga tcgacgtgca ccccgggcgg cacatagatg aacgacccgc
     2581 ccgaccacac ggccgagttg agggcggcga acttgttgtc gttgggtggg atgacggtcc
     2641 cgaagtgctc cttgacgatc tcgggatact cccggacggc ggtgtccatg tcggtgaaca
     2701 aaacacccag cttctccagg tcctcccggt tgcggtggta gacgacctcg ctctcgtatt
     2761 gagcggtcac cccggcgagg tacttgcgct cggcttcggg gatgcccagc ttctcgtagg
     2821 tctccttgat cgattccggg accatgtccc agtccttctg ctgctcacct tccggcttga
     2881 cgtagtagta gatgtcgtcg aagtcgatgg tgttgagcgc gccgccaccg ccccactgcg
     2941 ggatcggctt gcgcaggaag cgcttgtagg ccttgagacg gaaatcgagc atccactcgg
     3001 gctcccgctt catggcggac atgtcccgga tgacgtcctc gtccaggcct ttgcggggct
     3061 cgaaggcgag ctcgacttcg tctcgccagc cgagctggta tccccctagg tcgatatcaa
     3121 ctgtcgccac ttggcaatca cctcaaaaga gtgtcttcgt gtagttcaac aagcggaaag
     3181 cccgacgaat ttccactatc cggataggaa atatcctagg tgcgtgatgg ctcatcgtaa
     3241 aagtggcgct cggattcgct gcagcggcca ctactttgga tgacatgccg atgatcgtcg
     3301 tcggggcaga caccacgcca ggagccgtgc tgctcagcca gctcgacgct tcagctcgag
     3361 agataagggt cttcgtcacc gacgaggctc gtgggctcga actgaaggga gcgggtttca
     3421 aggtggccac cggtgatgtc tccgatgaca gccacgtgga ggccgctgcc acccgatgct
     3481 tctcggcagt tctcatttcg gaggctgccc atgacgatcg agagcgctcc tttgcccgta
     3541 atgctgccga tgtgctcgaa ggctgggcga aggcggtctc caactcgaag gtcaggaggg
     3601 tgatctgggt cacagacggt gactacccgg acacatctgt gccggaagtc gccgtagtcg
     3661 acccgtccga gaccgacttt gcgaggaaga tcgtcgatct cgacgaggcg gcgaagctga
     3721 gctgagccga ccgcccgtca ctccaccgga tacaccgacc ggtcagcctc cagcatcatc
     3781 gcgagatact gctccgcagc cacctcgcgc tcataggcgt cgatcaggat ctgatacggg
     3841 tcgatcgcag atccgtcgtg acgaatctcg aagtgagtgt gcggagccgt ccgctcggcg
     3901 ttacccgagt cacccaccca accgatgagc tggcccgcct cgaccgctgt cccttcttcg
     3961 atcccgggag cgactgtcaa cgaccaagga gcgtccccgt cgtcggttcc agggttgtcg
     4021 ttgttgaggt ggaggtagta gctctcccaa ccgcccccgt ggtcgatttt gacattcctg
     4081 cccgagagac gattgatccc gacatggaag accacgccgt cggccattgc atagacctcg
     4141 gtgagacggg gggccatcaa gtcgttcccc ttgtggtagc ggcctccgga tcggtagtcg
     4201 ccgtatgagg acgagaagta ggtcttcgag gtctcttgag ggaagacgac cacgaacggt
     4261 gggtcggact gtgcccgtgc cggg
//
LOCUS       DASUTA010000314         3330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1390248, whole genome shotgun sequence.
ACCESSION   DASUTA010000314 DASUTA010000000
VERSION     DASUTA010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390248"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFZ80_06270"
     CDS             complement(<1..435)
                     /locus_tag="VFZ80_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6287071.1"
                     /translation="MEPLTREQAFDLLGREPVAHLGLILDGEPCVYPMSYVLDGERIL
                     FRTMAGAKLDGIRANPRVCIEVSTFDDETGDWQSVIVRGTASFVDSDTARQNLIAQLF
                     AKYEKVMGSPLSAGGGLMPLGGQPHVIEVPIEEISGMSSHGGF"
     gene            complement(438..824)
                     /locus_tag="VFZ80_06275"
     CDS             complement(438..824)
                     /locus_tag="VFZ80_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6287072.1"
                     /translation="MELRELLSGEVITIGHDETVAEGAAEMVREGIGSLAVIADDEIY
                     GIFTERDVVRCVAAGYDSVDAPIGEWMTPYPDSFSPDMDVNEAADWMLAVGYRHLPVV
                     ENGHLIGMTSIKDILWALTEEAAKVR"
     gene            965..1330
                     /locus_tag="VFZ80_06280"
     CDS             965..1330
                     /locus_tag="VFZ80_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287073.1"
                     /translation="MDYDKWCEHKPVWVGRIAVAACEECGRVEWFSNEGPVDPAEAMA
                     ALFGSYDLMGSLDALGAPAPEVLAYSAPSARKRRNLDALPRKVWLKAAPHLWMSTDGV
                     TLLLAPTQRLMFDNLTRGA"
     gene            complement(1355..3169)
                     /locus_tag="VFZ80_06285"
     CDS             complement(1355..3169)
                     /locus_tag="VFZ80_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6287074.1"
                     /translation="MLLRVWQYGRPYLSGLLGILVTIVVISTLSVVPAILIRDIIDEA
                     IPNEDFRQLTFLGAGMIGVALVNALVGVIQRWLTSRVGEGIIFDLRSELYAHLQRMSL
                     RFFTATKTGELMNRLNSDVVGAQQAITGTFVTIVSNVISVTVILVVMLQEDWRLTLLA
                     IAALPLFVIPARRVAKVLRRITQQQMEYEARMSGILQESFNVSGAQLMRLFGRWAEER
                     ERFGGAAMLVRDYGVRRAMVGRGFFAALGLVSAIGTAAVFWAGAAFVINGEIEVGVVV
                     MFSTLLAQFYGPLSALTNSRVEFATSLVSFERVFEVLDLETDIAEPTSPKALSGVGRV
                     EFNDVWFRYQGDGPEGLAAVQRFSWHRANDTGAAVSQTSTRDWALEGVDFVAQPGSLT
                     ALVGPSGAGKTTISYLLPRLYDVTKGQVLVDGVDVRDIALLDLAGEIGVVTQETYLFH
                     DTIEANLRYARPDATREQLEAAARAANIDEMIDRLPDGYQTVVGERGYRLSGGEKQRI
                     AIARVVLKDPKILILDEATSHLDARSEALIQQALEQLMRDRTSIVIAHRLSTVRAADQ
                     ILVLDEGRIVERGDHESLVKADRLYAELNRTQFASADV"
BASE COUNT          655 a         1058 c         1016 g          601 t
ORIGIN      
        1 gaagccaccg tgcgacgaca tcccggagat ctcttcgatg ggaacttcga tcacatgggg
       61 ctggcctccg aggggcatca gaccaccccc ggcagacaag ggggagccca tgaccttctc
      121 atacttggcg aagagctggg cgatgagatt ctgtctcgcg gtgtcactgt cgacgaaact
      181 ggctgtgccg cgcacgatga cgctctgcca gtcacctgtc tcatcgtcga aagtggagac
      241 ctcgatgcaa acccgcggat tggcccggat tccgtccagc ttggcgccgg ccatggtcct
      301 gaacaggatt cgctcaccgt ccaggacata tgacatcggg tacacgcacg gctcaccatc
      361 gaggatcaac ccgaggtgag ctaccggttc gcgaccgagc aggtcgaatg cctgttctct
      421 ggtgagcggt tccatggtca gcgaaccttt gccgcctcct cagtcagtgc ccagaggatg
      481 tccttgatcg aggtcatccc gatcagatgc ccgttctcga cgaccgggag atggcgatat
      541 cccaccgcca gcatccagtc cgccgcctcg ttcacatcca tgtccggtga gaaggaatcc
      601 ggatacggag tcatccactc cccgatcggg gcgtcgacgg agtcatagcc ggcggcgaca
      661 caacggacca catcgcgctc ggtgaatatc ccgtatatct catcgtcggc gatgacggcg
      721 agcgacccaa tgccctccct gaccatttcg gcggcgcctt cggcaaccgt ctcgtcgtga
      781 ccgatcgtga tcacttcacc gctcaacagc tctcgaagct ccatcacaac ctccttggac
      841 tctctgtgag ctgcctcgat tctccagcag gccggggacc gatgacatgg tcgaaagtcc
      901 ccatcgcatg ggacttcccg ccacacgaga attcctgtca cacccggctt ctactttgat
      961 ggccatggac tacgacaagt ggtgcgagca caagccggtg tgggtggggc gcatcgcagt
     1021 ggcggcgtgt gaagaatgcg gacgggtgga gtggttctcc aacgagggcc ccgtcgaccc
     1081 ggccgaggcc atggcagccc tgtttggcag ctacgacctc atgggctctc tcgacgccct
     1141 cggcgcaccc gcccccgagg tgctcgccta ttcggctccc tcagctcgca agagacgcaa
     1201 cctcgacgcc ctcccccgca aggtgtggct gaaggcggcg ccccatctct ggatgtcgac
     1261 cgacggtgtg acgttgctat tggcacccac acagcgtctc atgttcgaca acttgaccag
     1321 aggcgcctga gtgagctgag gacctaccgc gccgctagac gtcggcggat gcgaactgag
     1381 tgcggttgag ctcggcgtag agacggtcgg ctttcaccaa tgactcgtga tctccgcgct
     1441 cgacgatccg accctcgtcg agaaccagga tctggtcagc agcgcgaaca gtggagagtc
     1501 gatgggcgat cacgatcgac gtccgatccc gcatcaactg ctccagagcc tgctggatga
     1561 gtgcctcgga ccgggcgtcg agatgggagg tggcctcgtc gaggatcaga atcttcggat
     1621 ccttcaacac cacccgggca atggcgattc gctgcttctc tccccctgag aggcggtaac
     1681 cgcgttcacc gacgacggtc tgatacccgt ctgggagccg gtcgatcatc tcgtcgatgt
     1741 tggcggcccg ggcggccgcc tccagctgtt cccgggtggc gtcgggtctg gcgtagcgga
     1801 gattggcttc gatggtgtcg tgaaagaggt aggtctcctg ggtgacgact ccgatctcac
     1861 cagcgaggtc gagaagagcg atatcgcgca cgtccacacc atcgaccaag acctgaccct
     1921 tggtcacgtc gtagagacgg ggcaagagat atgagatggt cgtcttgccg gcgcccgacg
     1981 ggcccacgag ggcggtcagc gatccgggct gggccacgaa atcgacgcct tcgagggccc
     2041 agtccctggt cgacgtctgg gaaaccgccg ctcccgtgtc attggcccga tgccagctga
     2101 acctctgcac tgccgccagg ccctcgggac cgtcgccctg ataccggaac cacacatcgt
     2161 tgaactccac ccttcccaca ccggagaggg ccttcggact cgtgggctcg gcgatgtcgg
     2221 tctccagatc gagcacctcg aagacccgct cgaatgagac gagcgaggtg gcgaattcga
     2281 ctctggagtt ggtgagggcc gacagtgggc catagaactg ggccaagagg gtggagaaca
     2341 tgaccaccac tcctacctcg atctcgccgt tgatgacgaa agcggctccg gcccagaaga
     2401 cggcggcggt gcctatcgcc gacaccaacc cgagggctgc gaagaagccc cgtcccacca
     2461 tggcgcggcg cactccatag tctcgaacca gcatggctgc cccgccgaaa cgctctcgtt
     2521 cctcggccca tctcccgaag agccgcatca gctgggctcc tgacacattg aaggactcct
     2581 ggagtatccc cgacatgcgt gcctcgtact ccatctgctg ctgggttatg cgacgcaaga
     2641 ccttggcgac acgacgggct gggatcacga agagaggcag ggctgcaatc gccagaagag
     2701 tcagtcgcca gtcttcttgc agcatcacga cgaggatcac cgtgacggag atcacattgg
     2761 agacgatggt gacgaacgtc cccgtgatcg cctgctgagc acccacgaca tcactgttca
     2821 gccggttcat gagctcgccg gtcttggtgg ccgtgaagaa gcgaagggac atgcgttgca
     2881 gatgggcgta cagctctgag cgcagatcga agatgatccc ctctccgacc cgggaagtca
     2941 gccaccgttg gatcacgccc accagggcgt tgaccagggc gacgccgatc atgccggccc
     3001 cgaggaaggt cagctgtctg aagtcctcgt tcgggatggc ctcatcgatg atgtcgcgga
     3061 tcaagatggc gggcaccacc gaaagggtcg agatcacgac gatcgtgacc agtatcccca
     3121 gcagaccgga gagatagggg cgaccgtact gccagacgcg taggagcaac gcccgatcga
     3181 tattcgggcg atcctgctct tcgtcgtact tcaggtagct ccaccagcca ccagagtgat
     3241 agatgcatgc ctcctggctc ggccaggaaa ggctatcgac aggatcctgc taccggacag
     3301 gtgcgggtca tgaggggatg gttgtcaggc
//
LOCUS       DASUTA010000315         3091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1260091, whole genome shotgun sequence.
ACCESSION   DASUTA010000315 DASUTA010000000
VERSION     DASUTA010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260091"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFZ80_06290"
     CDS             complement(<1..309)
                     /locus_tag="VFZ80_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287075.1"
                     /translation="MREGGSRWLLSAPMIALLVIGAAVSAAVPALAPTIGFVGLAVAI
                     PVAVVLFSVRSRGLPKREQTAWRYMAIGLLLFWAGVVVIAALTESGVDLPAFGPLDGF
                     F"
     gene            complement(371..1636)
                     /locus_tag="VFZ80_06295"
     CDS             complement(371..1636)
                     /locus_tag="VFZ80_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6287076.1"
                     /translation="MFPPSHNMTQNIATPSARLPDGFLQQSLFAHESGLSSQVIEDKE
                     FTGDLITIKGRELYNFGLCSYLGLSDDPRLVEGAIDAIRRYGNSYSSSVAYTSLPLYD
                     ELRERLSAMIGAPVMVAGTTTLAHMAALPVLVRSGDLVLVDRLAHASLLGVIPTLQAR
                     RAKVERVPHNDLDLVSQLVRSHDGPVWYVIDGLYSMHGDQAPADQLRELIDQHESLWV
                     YCDDAHGFSWSGRHGRGTFLDEFGWHPRLVMSYGLSKSFGALGGVVAAQDPNLIRAIE
                     ITGGPMVFGGPVPPASLGAGVVSGDIHLSSELPILQRDLYARIDFVNEYAGRIGLPLS
                     AREPTPLWFVEIGGTSTTASVGGRIMRDGYFVNVATYPVVGKGRSGVRFTVTRYNTIS
                     QIRGLLDSINDARLQYDRDEDVLDLTVFE"
     gene            1749..2102
                     /locus_tag="VFZ80_06300"
     CDS             1749..2102
                     /locus_tag="VFZ80_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287077.1"
                     /translation="MTLQPTDLPPVAAAAFHDLTEEQKAAFVSAYEAKRRSMPLMMAF
                     AILLPIQLFLLGKVLLGILFLLTGGGLGVWYLIEWFMTPQRVRDYNTKVAGETMTLVK
                     AGSDTAAAIAEPEED"
     gene            2126..>3091
                     /locus_tag="VFZ80_06305"
     CDS             2126..>3091
                     /locus_tag="VFZ80_06305"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX6287078.1"
                     /translation="MSPVLARSLVFFTSAAVLVIEILAARMLAPYLGVSLEVFTGIIG
                     VILAGISVGAWLGGRSADRTDPLRLPGPLLIAGGLTALASPLIIDTIGPSLRINAVSI
                     VFAAAAGFFVPAAILSAVPPVIVKIQLGSLDETGRVVGTYSAIGTAGAILGTFVTGFV
                     LIAAFPTRPIVVVLGAALTVTGAAISLTRGSWTVMSVAAAGVLGFALVAVGGPCEYET
                     TYHCAIVEEDPANSSGRTLVLDRLRNSYVDLDDPTYLEFRYIKLIADIIEVEAAEGAL
                     EVVSIGGGGFTMPGYVEETRPGSTNTVLEIDSKLVDIGYDELALTE"
BASE COUNT          580 a          971 c          968 g          572 t
ORIGIN      
        1 gaagaaaccg tcgagcgggc cgaacgcagg cagatcgact ccggattcgg tgagggctgc
       61 tatcacaacg actccggccc agaagagcaa cagcccgatc gccatgtatc gccatgccgt
      121 ctgctctctc ttgggaagac ctcgggagcg gacagagaag aggacgacgg cgactgggat
      181 agccacggcg aggccgacga acccgatagt gggggcgagc gcgggcacgg cggcagacac
      241 tgctgctcca atgacgagca gcgcgatcat tggcgcggag aggagccagc gactgcctcc
      301 ctctctcatc gagataccca cgacgtctta tcggaccgtt tcggggtctt attgagccgg
      361 gaagggtgat ctactcgaag accgtcaggt cgaggacgtc ctcgtcgcgg tcatattgga
      421 gtcgagcatc gttgatgctg tcgaggagcc ctcgtatctg actgatcgtg ttgtagcggg
      481 tgaccgtgaa ccggacgccg gaccgaccct tgcccacgac cgggtacgtt gcgacgttca
      541 cgaagtatcc gtcgcgcatg attcgacccc ccaccgatgc cgtggtgctg gtgccaccga
      601 tctcgacgaa ccagagcggt gtcggctccc gggctgagag aggcaggcca atgcgtcccg
      661 catattcgtt gacgaagtcg attcgcgcgt agagatcccg ctgcaggatg ggcagttcgc
      721 tggagagatg gatgtctccg gagacgacac ccgcgccgag gctggccgga gggacggggc
      781 caccgaagac catcggccca ccggttatct cgatggccct gatcaggttg gggtcctggg
      841 cggccaccac tcccccgaga gcaccgaacg acttggacag cccataggac atgaccagtc
      901 gcggatgcca gccgaactcg tcgaggaacg ttccccgacc gtgtcgaccg gaccaggaga
      961 acccgtgggc atcgtcgcag tagacccata gggactcgtg ttggtcgatc agctcgcgca
     1021 attggtccgc gggcgcctgg tctccgtgca tcgagtagag cccgtcgatg acgtaccaga
     1081 ccggaccatc gtgggagcga acgagctggg acacgagatc caggtcgttg tgcgggacgc
     1141 gctcgacctt ggctctcctc gcctggagtg tcgggatgac cccgagcagc gaggcgtgag
     1201 ccaaacggtc gacgagcacg aggtcgccag atcgcacgag caccggaagc gcggccatat
     1261 gcgccagagt cgtcgtccct gcaaccatga ccggggctcc gatcatcgcc gaaagacgct
     1321 ctcgcagctc gtcgtagagg ggaagcgatg tgtaagccac cgacgacgag tacgagttcc
     1381 catagcgtcg gatcgcgtcg atggcccctt cgacaagcct ggggtcgtcg ctcagcccca
     1441 ggtaggagca gagaccgaag ttgtagagct cacggccttt gatcgtgatc aggtcaccgg
     1501 tgaactcctt gtcctcgatg acctgcgagc tcagacccga ctcatgggcg aatagggact
     1561 gttggagaaa cccgtccgga agacgggccg agggcgttgc gatgttctgg gtcatgttgt
     1621 gcgacggggg gaacatcaaa ctaggccacg tgtcaaggct ataggtgtca atccttcacc
     1681 caccgcaaca gatgcccaca gagagtgaga gagacgtcgc tcatcacgat gtcggatatc
     1741 cttgggccat gactcttcaa ccgacggatc tgcctccagt tgctgcggcc gccttccacg
     1801 atctgaccga agagcagaag gcagcgtttg tctccgccta tgaagccaag cgacgcagca
     1861 tgccgctgat gatggcgttt gccatcctcc tccccatcca gttgttcctc ctgggaaagg
     1921 tgctcctggg aatcctcttc ctgctcaccg gtggcggact cggcgtctgg tatctgatcg
     1981 agtggttcat gaccccgcag cgggtgcgtg actacaacac caaggtcgcc ggcgagacca
     2041 tgaccctggt gaaggctgga tctgacaccg cggccgctat cgccgagccc gaagaggact
     2101 agctccagcc ctaccctgac cggacttgag ccctgttctc gcccgctcgc tcgtcttctt
     2161 cacatccgcc gccgtgctgg tcatcgagat cctggcggca cgaatgctgg ccccgtattt
     2221 gggtgtaagt ctcgaggtgt tcaccggcat catcggcgtg atcctcgccg ggatatctgt
     2281 tggtgcctgg ctcggtggcc gctctgcaga ccggaccgac cctctgcgtc ttcccggacc
     2341 cctcctcatc gccggaggcc tcaccgcgct ggcctcgcct ttgatcatcg acacgatcgg
     2401 cccctcgctg cgaatcaatg cggtatcgat cgtctttgct gcagccgcag gtttcttcgt
     2461 gcctgcagcc atcctctccg cggtcccgcc cgtgatcgtc aagatccagc tcggttcact
     2521 cgacgagaca gggagggtgg tgggcaccta ctcggcgatc gggaccgccg gggcgattct
     2581 ggggaccttc gtcaccggtt tcgttctcat tgcagccttc cccacgagac ccatagtcgt
     2641 cgtcttggga gcagccctca ccgtgaccgg ggccgccata tcactgacac gaggcagttg
     2701 gactgtgatg tcggtggcgg cggcaggagt cttgggcttc gccttggtcg ccgttggcgg
     2761 cccctgcgag tacgagacca cctaccactg cgccatcgtc gaggaagacc ctgcgaactc
     2821 gtcggggcgg acactggtcc tcgatcggct ccgcaacagc tacgtcgatc tggatgatcc
     2881 gacgtatctg gaattccgct acatcaaact gatcgccgac atcatcgagg tcgaggcagc
     2941 cgaaggagcc ctcgaagtcg tctcgatcgg cgggggtggc ttcaccatgc cgggctatgt
     3001 cgaggagacg aggcccgggt ctaccaacac cgtcctcgag atcgactcga agctggtcga
     3061 catcggctac gacgagctcg ccctcaccga a
//
LOCUS       DASUTA010000316         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_354474, whole genome shotgun sequence.
ACCESSION   DASUTA010000316 DASUTA010000000
VERSION     DASUTA010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354474"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..754)
                     /locus_tag="VFZ80_06310"
     CDS             complement(236..754)
                     /locus_tag="VFZ80_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287079.1"
                     /translation="MLHSDSSQLVKIYLDDHWAGAGAGLNLARRMMRNNQGTAWIDDL
                     SWVAAQIEADHRVLADIRKRLGVEGGGVKRRLAVLGERVARLKPNGRIIGYSPLSRVL
                     EAEALISGVVAKRNLWAGLHQGLVGVEFPGVDFESLQERADEQLELLRSIHREAVSLA
                     FGADAHRRVVSS"
     gene            962..1312
                     /locus_tag="VFZ80_06315"
     CDS             962..1312
                     /locus_tag="VFZ80_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX6287080.1"
                     /translation="MVPRGARQGQTTQLLHGVLEMCLLSIIEEEASYGYEMVSKLRER
                     GLDLASEGSIYPLLSRLQKQGLIEGYLVQSSEGPARKYYRMSDQGRKTLEQWKLVWRE
                     FSRSVDAVLEGGTR"
     gene            1309..2088
                     /locus_tag="VFZ80_06320"
     CDS             1309..2088
                     /locus_tag="VFZ80_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX6287081.1"
                     /translation="MTDRSHALAEIEHYWLETGLTPDSVEEMKVELEQHLIEAELDGR
                     SVDDVIGNRAAFAEGWAAARRGRSVETWADVQSGRTKRRKETRRDLVLYGSGAVAMIA
                     AVAVATQGGSDVDNELWRWMWTIFAIVLAIGEMFTAGFFLLPFAIGGAAAAILAWLGA
                     NVLAQWLVFFGVSTISLVYLRRFIGRQDEGEQPRVGANRWIGSEGVVLQGIDPHSGSG
                     MVRILNEEWRATAPGKIPAGTKIIVTQVDGARLIVEALEEV"
BASE COUNT          444 a          593 c          655 g          419 t
ORIGIN      
        1 gaggcatgat tgtgatcacg ggccaggttg gtcgccgtga cgagggcttc ccttgttcgt
       61 gcagtcagtt tctcaggatt cataggttgt gagaattgta atagaaaacc tgagtgctgt
      121 catatcaagt taggcaaccg atcgtaacgg gtcatcgacg aatggccccg ggggatctcg
      181 ccaagcgact ccatttggcc ggatctcgat gttgccccgg cttgggactg atgagctacg
      241 acgaaaccac cctcctgtgc gcatctgcac cgaaggcgag ggagactgcc tctctatgaa
      301 tcgagcgaag aagctccagc tgttcgtcgg cgcgctcttg cagcgactcg aagtcgacac
      361 ccgggaactc cacccccacg agcccctgat gcaaaccggc ccacaggttg cgtttggcca
      421 cgacaccgga gatcaacgcc tcggcttcga ggacccggct caatggcgag tagccgatga
      481 tccttccgtt cggcttgaga cgagccaccc tctccccgag cacagcaaga cgtcgcttca
      541 caccgccgcc ttcgactcca agccgtttcc ttatgtcagc cagcacacgg tggtccgcct
      601 cgatctgggc tgcaacccag gagagatcgt caatccaagc cgtcccctga ttgttgcgca
      661 tcattcgcct tgccagattg agaccagccc cagcaccggc ccaatggtcg tcgagataga
      721 tcttcaccaa ctgggatgag tctgaatgaa gcatggccgt ccctctttga gtgcatcccg
      781 gtttacccgg agcccggggg ccacgaaaca acggagggat ggccggccag agcggcgatg
      841 gccccggtgg agatatcgag cggttgctgg aagattctca gatgctgcct tgtctgaata
      901 ggcgtcgctc tacatatctt gtgccattga cttcttagca ccatcgagta gcatgttgta
      961 catggtacct agaggtgcta gacagggtca gaccacccag ttgctccacg gggttctcga
     1021 gatgtgtcta ctttcaataa tcgaagagga ggcctcctac ggctacgaga tggtcagcaa
     1081 gttgagagag cgcggtctcg acctggcgag cgaaggctcg atatatcccc ttctctccag
     1141 acttcagaaa cagggtctga tagaggggta tctggttcag agctcggagg gccccgcccg
     1201 caagtactac aggatgtccg accagggtcg gaagacccta gagcaatgga agctggtttg
     1261 gcgcgagttc agccgatcgg tggacgcagt acttgaaggg ggaacgagat gacagacaga
     1321 agtcatgccc tcgccgagat cgagcattat tggctggaga ccgggctgac cccggacagc
     1381 gtcgaggaga tgaaggtcga gctcgagcag catttgatcg aagccgagct cgacgggcgc
     1441 agtgtcgacg atgtcatagg gaaccgcgcc gctttcgccg aaggctgggc ggccgcgcga
     1501 agaggcagat cagtcgaaac ctgggcagat gtccagagtg gaagaaccaa gagacgaaag
     1561 gaaacccgtc gggacctggt cctgtatggg tcgggagcgg tagcgatgat cgccgccgtt
     1621 gccgtcgcaa cacaaggagg tagcgacgtg gacaatgaac tttggcgttg gatgtggacg
     1681 atcttcgcca tcgtcctcgc aatcggggag atgttcaccg ccggcttctt cctgctgccg
     1741 tttgcgatcg gaggtgctgc cgccgccatc cttgcgtggc tcggagccaa cgtgttggcg
     1801 caatggctgg tcttcttcgg tgtgtcgacg atctccctcg tatacctgcg tcgcttcatc
     1861 ggccgccagg acgagggcga gcagcctcgt gtaggggcca acaggtggat cgggtcggaa
     1921 ggcgtcgtcc tccagggcat cgatccgcac tcgggctcgg gaatggttcg catcctcaac
     1981 gaggagtgga gagccactgc tcccgggaag attcccgctg ggacgaagat catcgtcact
     2041 caggtcgacg gggcgcggct catagtcgag gccctggaag aagtttgaaa gacaagtccg
     2101 aattttcgag a
//
LOCUS       DASUTA010000317         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1778782, whole genome shotgun sequence.
ACCESSION   DASUTA010000317 DASUTA010000000
VERSION     DASUTA010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1778782"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VFZ80_06325"
     CDS             complement(<1..558)
                     /locus_tag="VFZ80_06325"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEX6287082.1"
                     /translation="MFDTLTERFESVFSTLRGKGKLSEKDVDAALREVRLALLEADVN
                     VSVVKAFLGRVRDRALGEDVSKSITPGQQVIKIVHEELVNTLGSETADLVKTSPPLVI
                     LMVGLQGSGKTTTAGKLAMHLQAKGKRPLLVAADLQRPAAIDQLETLGQRVDVPVLVD
                     RKGKAAKLAKSALKEAEKTGRDVIII"
     gene            604..1011
                     /locus_tag="VFZ80_06330"
     CDS             604..1011
                     /locus_tag="VFZ80_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018788660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6287083.1"
                     /translation="MEKVNGIGGVFFRAQDPEALAVWYETHLGVRKTPESYEEGSWWQ
                     EKGPTVFAPFESDTDYFGDTVRQWMINFRVDDLDAMAAQLSDADIDVDVVPETFPNGR
                     FAHLTDPEGNRIELWEPGGTDLTEPETHRRTDA"
     gene            1021..1899
                     /locus_tag="VFZ80_06335"
     CDS             1021..1899
                     /locus_tag="VFZ80_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX6287084.1"
                     /translation="MIPNTIEELKRSLDSQDYFAGDDLTVAIHLALQLGRPLFLEGEA
                     GVGKTEVAKVLASLAGEDLIRLQCYEGLDVSHALYEWDYALQMMEIRLLEARGEGERA
                     TAKDIMSRDFLIARPLLQAIEAARGGRRPVLLIDELDRADEEFEAFLLELLSDFQVSI
                     PEIGTIKADDPPIVVLTSNRTREIHDALKRRCIYHWIDYPTPDLEASIVFRKVPGIEG
                     ELASSLAAAMFKLRQMELFKPPGVAETLDWAESLQLLHDGGLTPAMIERTLGVVLKYK
                     DDIDTVRDNGLEALVG"
     gene            1896..>2099
                     /locus_tag="VFZ80_06340"
     CDS             1896..>2099
                     /locus_tag="VFZ80_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287085.1"
                     /translation="MRPGALTDLTGFATALRRRGLTVTPDQVSDMARALTLVDPSRRS
                     QVHASLRALAITDPDQRLPFDEEF"
BASE COUNT          410 a          674 c          630 g          385 t
ORIGIN      
        1 gatgatgatc acgtcacggc ccgtcttctc tgcttccttc agcgcggact tggcgagctt
       61 ggctgccttg cccttgcggt cgacgagcac cggcacatcc acgcgctggc ccagggtctc
      121 gagctgatcg atggcggcgg gccgttgcag gtccgcggcc acgagaagcg gccgcttccc
      181 tttggcctgc agatgcatgg ccagcttccc tgcggtcgtc gtcttacccg agccctgcag
      241 ccccaccatg agaatcacca gcggcggaga cgtcttgacg agatcggcgg tctcgcttcc
      301 cagggtgttc accaattcct catgcacgat cttgatcacc tgttggccag gtgtgatcga
      361 cttggacacg tcctcgccca gtgcccgatc tctgaccctg ccgagaaatg ccttgaccac
      421 ggacacgttc acatcggcct cgagcaacgc cagacgcacc tcccgcaaag cggcatcgac
      481 gtctttctcg gagagcttgc ccttgcctcg aagagtggaa aagacggatt cgaatctttc
      541 ggtgagcgtg tcgaacatcg gctctatagg ttatggccca tgtactttca atcggagcgc
      601 tgagtggaga aggtcaacgg catcggtggc gtgttcttcc gagcacagga cccagaggca
      661 ctggccgtct ggtacgagac acacctcggc gtgcggaaaa cgcccgagag ctacgaggaa
      721 ggctcttggt ggcaggagaa aggccccacc gtcttcgcac cgttcgagtc ggacaccgat
      781 tacttcggag acaccgtgag gcagtggatg atcaacttca gggtggacga tctcgatgcc
      841 atggcggccc agctgagcga tgcggacatc gatgtcgatg tcgtgccgga gacatttcca
      901 aacgggcgtt tcgcccatct caccgaccct gagggcaacc ggatcgagct ttgggaaccg
      961 ggcgggacag acctgaccga gccagagaca catcgccgca cggatgctta acctggcgat
     1021 gtgatcccga acaccatcga ggagctgaaa cgctctctcg acagccagga ctacttcgcc
     1081 ggcgacgatc tcaccgtcgc gatccacctc gccctgcagt tgggacggcc gctgtttctc
     1141 gaaggcgagg cgggagtggg caagacggag gtggcgaagg ttctcgcttc gctcgccggt
     1201 gaggacctga tcagactcca gtgttacgaa ggcctggacg tcagtcacgc cctctatgag
     1261 tgggactacg cccttcaaat gatggagatc agactcctcg aggcccgtgg cgagggcgag
     1321 agagcaacgg ccaaagacat catgagccgc gacttcctga tcgccagacc tctcctgcag
     1381 gcgatagaag ccgcccgcgg cggtcggcgc ccggtactcc tcatcgacga gctggatcgc
     1441 gccgacgagg agttcgaggc gttccttctc gagctcctgt cggacttcca ggtctcgatc
     1501 cctgagatcg gcacgatcaa ggccgatgat cccccgatcg tcgtactcac ttcgaaccgg
     1561 actcgtgaga tccacgacgc cttgaagaga cgctgcatct accactggat cgactacccg
     1621 acgcccgatc tcgaggcctc gatcgtcttc cgcaaggtgc ccgggatcga aggcgagctc
     1681 gcctcgagcc tggcagccgc catgttcaaa ctccgccaga tggagttgtt caagccaccc
     1741 ggggtcgccg agaccctcga ttgggcagag tcgctgcagc tgcttcacga cggggggctg
     1801 accccggcga tgatcgagag gactctcggg gtcgttctca aatacaaaga cgacatcgac
     1861 accgtgaggg acaacggcct cgaagccctc gtgggatgag accgggtgcg ctgaccgatc
     1921 tgaccggttt cgccacggct ctccgccgac gcggcctgac cgtgacaccc gaccaggtct
     1981 ccgacatggc cagggcattg accctggtcg acccatcgag gagatcccag gtccatgcct
     2041 cgctcagggc tctggcaatc accgaccccg atcaacgcct cccattcgac gaagagttc
//
LOCUS       DASUTA010000318         3322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1519226, whole genome shotgun sequence.
ACCESSION   DASUTA010000318 DASUTA010000000
VERSION     DASUTA010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519226"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..>3322)
                     /locus_tag="VFZ80_06345"
     CDS             complement(147..>3322)
                     /locus_tag="VFZ80_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287086.1"
                     /translation="SAGTRVLLGRSWESGGAPPYWPWVQAIRGYLRVAEPALLESQLA
                     GRATELVAMLPELDELIPAPSPVSLGEAASERFRLFDAASTFLINVASSEPVVIVLED
                     LQAADVPSLLLLDFLSNHIAEAAILIVATYRDVELSPDHPLTPILGQLHRLPATSELA
                     LRGLDPAEVALYLEAAAGRHAASTLSTALYGRTSGNPLFLSEAVRLMTMGASDVSLAS
                     LQRVAIPAEIRAVIQRRLDGLSTSCKELLVTLSVLGDEFDVEMAAKFADLQVDEMLER
                     IGEALTAGLVIDSRGGPGTFAFAHDLVRQALYASLPPATRVRLHRSAAAVLEMSDGGA
                     DSENLAELALHYFEAAAGGEHVKAVEYGRLAGEQAIRRLAYEEAVRLFEMAVQTLESS
                     EVSDPQTLGSLLLSLGDARVRAGDLERAGKAFLRAAHEARRSGDARLMARAAVGYGGR
                     FVWARAGSDSEMVPLLQDALVMLGGEDDKLRVRLLSRLACATRSVGDREHGAALARQG
                     VALAREIGDPETLIYALSGLAGAIWWPENPKERIDLGTEMISVGESAGIVDGEIDGHM
                     TLCSAYAELGDFAAAREELRLLSRAGGSLRLDTQRWLEGAMSGVFALSDGGFARAETL
                     IDEMLHKAAVTPARDNIAAASFQLFLLRREQARLPEIEPSVRLAAEEFTWYPIHQLAL
                     AELLRLTGRLGEARSMLSELGAGGFARFHRDNYWIPSMCLASELVTGLGEVDLARVLM
                     DVLAPFSHLNAIGFPEGSLGAVARYVALLATLLGDHEVALATFDEAVAANERNGALPW
                     LAHTYYDKAKTLIDRGSPDDLVTAQGLLADSLELCEEVGLVALGESVRVAMSPDARSG
                     PVEATANGRDVFRREGDYFSISFRGDVFRLKDAKGLRYLSSLLAAPGREIHVLDLVAG
                     ERGVEPTRRGMVSDLDGLHDGGNAAPVLDRVARQAYESRLIEIEDDIDEATRFGDQER
                     VANAMFEREFLVNELAGAIGIGGRDRAAASDAERARINVTRAIRAAVAKVDSNSRALG
                     AHLDATVRTGVFCSYNPDPLQTVTWYT"
BASE COUNT          631 a         1112 c         1008 g          571 t
ORIGIN      
        1 ggtgtgggaa tgtccaccct cgtagtttcg gtgatcatga gcgcctcctc gtgctctcgc
       61 cagccggtgc ggctggtctc tacccaggag gcgttcgaaa cgaaggaacg agtgttcagt
      121 cggagcgaac atcggattga acgatttcag gtataccaag tgaccgtctg gaggggatcc
      181 gggttgtacg agcagaagac cccggtccgg accgtcgcat cgaggtgggc gcccaaagcc
      241 cggctgttcg agtccacttt cgccactgcg gcgcgaatcg cccgggtcac gttgattcgc
      301 gctcgctcgg catcggatgc cgcagctcta tcccgccctc cgatccctat cgcgccggct
      361 agctcgttga ctaggaactc tcgctcgaac atggcgttgg caactcgctc ctgatcccca
      421 aatcgcgttg cctcatcgat gtcgtcctcg atttcgatca gtcggctctc gtaggcttgg
      481 cgggcgaccc tgtcgagcac cggcgcggca tttccaccat cgtggagccc atcgaggtca
      541 ctgaccattc cgcgtcgcgt tggttcgaca ccacgttcgc cagctacgag atcgagtacg
      601 tgtatctctc gacctggggc ggccagcaac gaagacaggt atcgcagccc tttcgcgtcc
      661 ttgagcctga acacgtcgcc tcgaaaggag atcgagaaat agtccccttc gcgccggaat
      721 acatcgcgac cattggccgt cgcctcgact ggtcctgacc gggcgtccgg cgacatcgcg
      781 actcggaccg attccccaag agctacgagc ccgacctctt cgcacaactc cagcgaatcc
      841 gcgagtagtc cctgtgcggt cacgagatcg tcgggtgagc cgcgatcgat cagcgtcttc
      901 gccttgtcgt agtaggtgtg ggcgagccac ggcaatgcgc cgtttcgctc gttggcagcg
      961 acagcctcat cgaaggttgc gagggccact tcgtgatcgc cgagaagtgt cgccaacagc
     1021 gccacatagc gcgccaccgc ccccagggat ccctccggga atccaatcgc gttcaagtgc
     1081 gaaaacggtg cgagcacgtc catgagcact ctggcgaggt caacctcgcc gagtccggtc
     1141 accaactcgg atgccagaca catggaaggg atccagtagt tgtctcggtg gaacctggcg
     1201 aatccccctg caccgagctc actcagcatc gagcgggcct ctccaagcct gccggtgagc
     1261 ctcagcagct cggccagggc aagttgatgg atcgggtacc acgtgaactc ttcggcagcc
     1321 aggcgtaccg acggctcgat ctcgggcagc cgggcttgct cccgtcgaag caggaagagt
     1381 tggaaagacg ccgcagcgat gttgtcccga gcgggggtga ctgccgcttt gtgcaacatc
     1441 tcgtcgatca gggtctctgc cctggcaaat ccaccgtccg acagtgcaaa gactccgctc
     1501 atcgcaccct cgagccaacg ttgggtatct agccgcagcg acccgcctgc cctactgaga
     1561 agacggagct cctctctcgc cgccgcaaag tcgcccagct cggcgtaggc ggagcagagg
     1621 gtcatgtgcc cgtcgatctc cccgtcgacg attccagccg actctcccac agagatcatc
     1681 tccgtaccga ggtcgatccg ctccttggga ttctcgggcc accaaatggc gcccgccaac
     1741 ccggacaggg cgtagatgag cgtctccggg tcgccgatct cacgagcgag ggcgacgccc
     1801 tgacgggcca gcgcagctcc gtgctcgcga tcgcccacgc ttcgagtggc gcacgcaagg
     1861 cggctcaaca gcctcacgcg aagcttgtcg tcctcgcctc ccaacatgac cagggcatct
     1921 tggaggaggg gaaccatctc cgagtcgctc cccgcacgcg cccacacaaa ccgccctcca
     1981 tatccgactg ccgcccgagc catcagacgg gcgtcaccgg agcggcgcgc ctcgtgcgcg
     2041 gctcgcagga acgccttgcc tgctctctcc aggtccccgg cgcgcacacg tgcatctccc
     2101 agactcaata gcagcgagcc caaggtctga ggatcggaga cctccgagct ctccaacgtc
     2161 tgaaccgcca tctcgaagag gcgaacggct tcctcgtacg ccagtctccg aatcgcttgc
     2221 tcccctgcca gccgaccgta ctcgacggct ttgacgtgct caccaccggc ggcagcttcg
     2281 aagtagtgaa gcgccaattc ggcgagattc tcactgtcgg ctccgccatc gctcatctcg
     2341 agaacggcag ccgcacttcg gtggaggcgc actctcgtcg ccggagggag actcgcgtaa
     2401 agcgcctgtc gcaccaggtc gtgcgcaaag gcgaacgtgc ctgggccacc acgagagtcg
     2461 atgacgagac cggccgtcaa cgcctcaccg attcgctcga gcatctcgtc gacttgcagg
     2521 tcagcgaatt tcgctgccat ttcgacgtcg aactcgtcgc ccagcaccga gagtgtcacg
     2581 agcagctcct tgcaggaggt cgacagcccg tcgaggcgcc gttgaataac ggcccgaatc
     2641 tccgctggta tggccacccg ctgcagtgac gccagagaga catccgatgc acccatggtc
     2701 atcaggcgaa ccgcctcaga gaggaacaac ggattgccac tcgtgcggcc atacagggca
     2761 gtggacaggg tcgacgccgc gtgtcttccc gctgcggcct cgaggtacaa cgccacctca
     2821 gccgggtcta ggccacgcag cgcgagctcg gacgtggcgg ggagacggtg gagttggccg
     2881 agtatcgggg tcagggggtg atcaggcgac agctcgacgt cccgatacgt cgcaacgatc
     2941 agaatggccg cctccgctat gtgattgctg aggaagtcga ggaggaggag cgacgggacg
     3001 tctgccgcct ggaggtcctc gaggacgatc acgaccggtt cggaagaagc cacgttgatg
     3061 agaaaggtcg aagcggcgtc gaacaaccga aatcgctctg acgcagcttc gccgagagaa
     3121 acagggctcg gtgcgggaat gagctcatcc aactcgggca gcatcgccac gagctccgtg
     3181 gctctcccgg cgagctggga ctcgagcagc gcaggctcag caacgcgcag atacccccgg
     3241 atggcctgga cccatggcca atatgggggt gctccgccgg actcccagct cctcccgagg
     3301 aggacccgtg tgcccgcaga cg
//
LOCUS       DASUTA010000319         2857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_613237, whole genome shotgun sequence.
ACCESSION   DASUTA010000319 DASUTA010000000
VERSION     DASUTA010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613237"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VFZ80_06350"
     CDS             <1..649
                     /locus_tag="VFZ80_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEX6287087.1"
                     /translation="RFLSVQSVDHAASIVVHERVKIGRDATSRVGEVGLGADLGRLDL
                     AVNLDGDGASSDVVGLYFGEGSQTLDYRMVINHVGRNTNSDVFLKGGLEDDSQSVFTG
                     LLRIEKGAEKTSTFETNRNLVLSANAKAHSVPNLEILCNDVICGHASSVGPLEEEHLY
                     YLQSRGLRKERAERLLIRGFFTEVIDRLPIAGLAEPVSTEVFRRFVEAQEEGRLS"
     gene            646..957
                     /locus_tag="VFZ80_06355"
     CDS             646..957
                     /locus_tag="VFZ80_06355"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HEX6287088.1"
                     /translation="MKVQVTELEKLPRGRGVRVTIGDTRIAMFRIEDDVYAIGDRCSH
                     AEASLAEGEIWDTSVECPRHGSEFDLKTGEPHALPATEPVPVYEVSIEDGTVYLELEN
                     G"
     gene            954..1760
                     /gene="sufC"
                     /locus_tag="VFZ80_06360"
     CDS             954..1760
                     /gene="sufC"
                     /locus_tag="VFZ80_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX6287089.1"
                     /translation="MSTALAVKDLDAAIDGKQILNDLSIEVPFGEIHALMGPNGSGKS
                     TLCHVLTGKPEYEVLDGTAFVDGSDIMTLDVDERSRVGLLQSFQYPTEVPGVRLREFL
                     LVAADERGIPADEAEARLNQEAKRFGMEPFLDRSLNEDLSGGEKKRSEIFQMAVLRPR
                     MAMLDEIDSGLDIDAVKQVAEAVEGMRSPELGVLIITHYSRILRYLTPDRIHVMIDGR
                     IVDSGGKELAEELEAGGYEAVRERLGIERPVTNEPTRKPADFFTDTPFDV"
     gene            1778..2851
                     /locus_tag="VFZ80_06365"
     CDS             1778..2851
                     /locus_tag="VFZ80_06365"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6287090.1"
                     /translation="MTTRATAEKWLSELTNTPTASGLEDAVVDWVEKWASRRDDIRIE
                     RDSGGNLFLTQKGRKSKPLVLAVAHMDHPAFVITDTDGAFEFRGGVDAVYFENARLDV
                     VSRLDGPTGQVTSYDEESATGTADFLGQVSPGDIAMWRFRNTRQKRGRFRAPACDDLA
                     GAAAALAALDRARGRRHLRHFGVMLTRAEEVGLIGAIHAAKNKTIPEDARLLSIETSR
                     ELPNARIGAGPIIRIGDRSTVFDREMSNRISQAAQGTGLAHQRKLMDGGGCEATAFGV
                     YGYRSTGLCLALGNWHNRGNLSAVESGDAAAAVPMLEEISVDDYHGLIDLLLLAAESL
                     DSAEGIVDRLDRLYEANRHYLSR"
BASE COUNT          555 a          884 c          908 g          510 t
ORIGIN      
        1 gcgctttctg tcggtgcaga gcgtcgacca tgccgcgtcg atcgtcgtcc acgaacgagt
       61 gaagatcgga cgcgatgcca cgtcacgagt cggtgaagtc ggacttgggg ccgacttggg
      121 gcgtctcgac ctggccgtca acctggatgg cgacggcgcc tcctccgacg tcgtcggcct
      181 ctacttcggc gaaggatccc agacgctcga ctaccgaatg gtcatcaacc acgtcggtcg
      241 gaacaccaac tcggatgtct tcttgaaggg cgggctcgaa gacgactccc agagcgtgtt
      301 caccggtctg ctccgaatcg agaaaggtgc cgagaagacc tcgaccttcg agaccaaccg
      361 caatctcgtc ctctccgcca acgccaaagc gcactccgtg cccaatctcg agattctctg
      421 caatgacgtc atctgtggcc acgcctcttc agtggggccg ctcgaggagg agcatctcta
      481 ctacctgcag agtcggggat tgcgaaagga acgtgcggag cggcttctca ttcgcggctt
      541 tttcaccgag gtgatcgatc gtctgccgat cgccggtttg gccgagcctg tctccaccga
      601 agtgttcaga cgcttcgtgg aggcacagga agagggtcgg ctttcatgaa ggtgcaggtc
      661 accgagctcg agaagctgcc acgggggaga ggcgttcgag tgacgatcgg cgacacccgg
      721 atcgccatgt tccgcatcga ggacgacgtc tacgccatcg gcgaccgctg ctcgcacgcc
      781 gaggcgtctc tggccgaagg cgagatctgg gacacgtcgg tggagtgtcc ccgtcacgga
      841 tcggagttcg atctcaagac cggagaacct cacgcgcttc ctgccaccga gccggtaccc
      901 gtctacgagg tctcaatcga agatgggacg gtctacctcg aattggagaa cggatgagca
      961 ccgccctcgc agtgaaggat ctcgacgccg ctatagatgg aaaacagatt ctcaacgatc
     1021 tgagcatcga ggtgccgttt ggcgagatcc atgcattgat gggcccaaat ggctccggga
     1081 agtccaccct ttgtcacgtt ctgacgggca agccggagta cgaagtcctc gacggcaccg
     1141 ccttcgtcga tggcagcgac atcatgaccc tcgatgtcga cgagcgttcc cgtgtggggc
     1201 tgttgcagag cttccagtac ccaaccgagg tccccggagt gagacttcgg gagttcctgc
     1261 tcgtagcggc agatgaacgc ggcatccccg ccgacgaggc agaggcgcgc ctgaaccagg
     1321 aggcgaagcg gttcggaatg gagcccttct tggaccggtc cctcaacgag gacctgtctg
     1381 gaggcgagaa gaagcgatct gagatcttcc agatggccgt gctccgtccc cggatggcca
     1441 tgctcgatga gatcgactcc ggtctcgaca tcgacgccgt gaagcaagtg gcggaagcgg
     1501 tggaaggcat gcgctcgccc gaactgggtg ttctgatcat cacccactac agccgcatcc
     1561 ttcgctacct gacgcctgac cgaattcatg tgatgatcga cggccgaata gtcgacagcg
     1621 gcggaaaaga gctggccgaa gagctcgagg ccggcgggta cgaggcggtg cgggagcgtc
     1681 tcgggatcga gaggccggtg acaaacgagc cgaccagaaa gccggccgac ttcttcacgg
     1741 acaccccctt cgacgtgtga gctccaaggg gtgagtgatg acgacccggg ccaccgctga
     1801 gaagtggctg tccgagctga ccaacacccc caccgcctca ggtctcgagg acgcagtcgt
     1861 cgactgggtc gagaaatggg cgagccggag agacgacatc cgcatcgaga gggactccgg
     1921 tgggaacctg ttcttgaccc agaagggccg gaagagcaag cccctggttc tggccgtcgc
     1981 tcacatggac cacccggcgt tcgtcatcac tgacacagat ggcgcgttcg agttcagggg
     2041 aggcgtcgac gccgtctact tcgagaatgc ccgtctagac gtggtctccc gtctcgatgg
     2101 ccccaccggt caggtcacgt cctacgacga ggagtcggcc accgggaccg ccgactttct
     2161 cggacaggtg tccccaggtg acatcgcgat gtggcgattc cgaaacaccc gtcagaagcg
     2221 cggtcgattc cgcgctccgg cgtgtgacga cctggccggc gcggccgccg cattggccgc
     2281 actagatcga gctcgcggcc gtcgccacct ccgacatttc ggcgtcatgc tcaccagggc
     2341 cgaggaggtc ggtctcatcg gcgccatcca cgccgccaag aacaagacga tccccgaaga
     2401 tgcccggctg ctgtcgatcg agacttcgcg agagcttccc aacgcccgaa tcggagcggg
     2461 gcccatcatc cgtattggcg atcggtcgac ggtcttcgac cgggagatgt ccaacagaat
     2521 cagccaggcg gctcagggga ccggccttgc acaccagcgc aagctgatgg atggtggagg
     2581 atgtgaggcc accgctttcg gcgtctacgg gtatcgatcg acgggtctct gtcttgctct
     2641 gggcaactgg cacaaccgtg ggaacctctc tgctgtggag tcgggagacg ccgcggcggc
     2701 cgtcccgatg ctcgaggaga tctcggtcga cgactatcac ggtctcatcg acctgctgct
     2761 gctcgcggca gagtccctgg acagcgccga gggcatcgtc gatcgtctgg atcggctcta
     2821 tgaggcaaac cgtcactatc tgtcgcggta gcgttcc
//
LOCUS       DASUTA010000320         4529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_613455, whole genome shotgun sequence.
ACCESSION   DASUTA010000320 DASUTA010000000
VERSION     DASUTA010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613455"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..844)
                     /locus_tag="VFZ80_06370"
     CDS             complement(68..844)
                     /locus_tag="VFZ80_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6287091.1"
                     /translation="MDVSIAGKVALVTGGSRGIGQATALEFAQSGAKGVVITSRRQEN
                     VDEARAELIEAGAPEDVVLALAARADDEDAAHEAVKATVTAFGSCDILVNNAGTNPAF
                     GNLMEVDLGALDKTWAVNMRAPLLWVRAAYDEWMRDHGGSIVNVASVAGLRPSPLMGA
                     YNISKAGLVHLTRQLALELAPRIRVNAVAPGVVKTRLAGALLEDEDATAAMHPLNRVG
                     DPEDVARLIVFLGSDASSWMTGAIVPIDGGVTGAGSSGIS"
     gene            complement(859..1380)
                     /locus_tag="VFZ80_06375"
     CDS             complement(859..1380)
                     /locus_tag="VFZ80_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX6287092.1"
                     /translation="MPGYAEEIVDPETINKVTWKIPNALALVGSAAGEEWNGMTTSWI
                     TQLSMEPVLIGIGVDNKAVTHRLITEGGSFTVNLWSAEDTRPFVKFSKPAEREGNSLN
                     GKPVSTGKTGAPIFDEAIAWLECEIRQSHDLGTHTLFIGEVVDAGIRDEEQRAAAMSD
                     TRMKYGGVKRGGH"
     gene            1401..1907
                     /locus_tag="VFZ80_06380"
     CDS             1401..1907
                     /locus_tag="VFZ80_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6287093.1"
                     /translation="MRILIATTGVLPAAPTADFCRQLIGDQTSVTVMTVIRVPRSFLE
                     SLGDEMRRSFLDSADNGASEEEKAAAYLEERGRRAVDPICAALAAEGIECDIRFVEGD
                     DPAEAIISTAEKVGAEMVVMGATRRLFTEEAWRSVSARVMVNSHLPVLLVPGARIEDT
                     MEMPVIEL"
     gene            1955..2362
                     /locus_tag="VFZ80_06385"
     CDS             1955..2362
                     /locus_tag="VFZ80_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019667966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX6287094.1"
                     /translation="MSNWNDGIVDEFRSNSGEVGGIFAGKPLLLLHHRGARTGRERVS
                     PLMYQRVGDSYAVFASKGGAETNPDWYHNLRAHPEVEIEVGEFTLDVRARVARGEEQD
                     TIWERQKREFPQFAGYEEKTSREQIPVIVLDPA"
     gene            complement(2380..3048)
                     /locus_tag="VFZ80_06390"
     CDS             complement(2380..3048)
                     /locus_tag="VFZ80_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017621150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6287095.1"
                     /translation="MTLRVSLIDDHEVVRAGLKALIDSQEDMTVVGEAGSVDEGVRRV
                     GFDEPDVVVLDVRLPDGSGVEACRTIRERFPDVKVLMLTSFADEEALMAAIMAGASGY
                     VLKRIKTEELVSDIRRVGAGESLLDPEMTDRLFTRLRQGDSEDPLLGQLTAQERTIVE
                     HIAGGMTNKQIAEEMFLAEKTVKNYVSNILAKMGMSRRSEAAAYVARIQAEKESSELP
                     ESWS"
     gene            3082..4203
                     /locus_tag="VFZ80_06395"
     CDS             3082..4203
                     /locus_tag="VFZ80_06395"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX6287096.1"
                     /translation="MVDRVAVTRLSPLIEEAAAVGGTADLDRLLRTLLSEAKTATGAR
                     YAALGVIGDHGVLSDFIYDGIGDDVAQQIGPPPSGRGVLGTLIRDRQSLILDVISGHP
                     DSVGFPEHHPPMATFLGVPISAGDESFGNLYLTEKDGGFTEDDLIAVKALSAIAGTAI
                     QSARLQRRLQQMAVVEDRQRIARDLHDSVIQDLFAVGLGLQALSLVVDEAVAASIDSA
                     IDTLDSSVATLRRYIFELKETPRPAQRLDERVRDVVERMSKAYSTRVELTVEDVHHGP
                     WVEDAVLLTTEALSNALRHGRADLIEISLRVEDDHQVIEVTDDGNGFDLGDVSTGMGL
                     SSMKARASATGGWADIESTPERGTRVTARLPIRPADPPG"
     gene            complement(4178..>4529)
                     /locus_tag="VFZ80_06400"
     CDS             complement(4178..>4529)
                     /locus_tag="VFZ80_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287097.1"
                     /translation="RQGEALQAKVGPTETLAKVVTLEIGVAEIHTYGLAYPVRWSAVG
                     ARLLFPELTADLILANAGKGATKLTLRGTYQPPLGALGRIADRAVLGRVAEATVTSWM
                     DRLAEALSPEGQRV"
BASE COUNT          819 a         1465 c         1417 g          828 t
ORIGIN      
        1 ccagagggca gcacctgcgg ctacgagcag gaatccagtt ccgatcttct tcattacctg
       61 gtctttctca actgatgccg gacgagcccg cgcctgtcac cccgccgtcg atcgggacaa
      121 tcgccccggt catccaactg gaggcgtcgg atcctagaaa gacgatgagc cgggcgacat
      181 cctcaggatc cccgacgcgg ttcaacgggt gcatggcggc ggttgcgtct tcgtcttcga
      241 gcagggcgcc ggcgagcctc gtcttgacca ctcccggcgc tactgcgttg actcgtatcc
      301 gaggagcgag ctcgagggcg agctggcggg tcaggtgaac caagccggct ttggagatgt
      361 tgtaggcgcc catcaatggg ctggggcgga gccccgctac cgacgcgacg ttcacgatgg
      421 agccgccgtg gtcacgcatc cactcgtcgt aagccgccct gacccaaagg agaggagcgc
      481 gcatgttgac cgcccaggtc ttgtcgaggg cgcccaggtc gacctccatc aggttgccga
      541 acgccgggtt ggtgccggcg ttgttcacca ggatgtcaca cgatccgaaa gctgtcaccg
      601 tggccttcac cgcttcgtgg gcagcatcct cgtcgtcggc gcgcgccgcc aaggccagaa
      661 ccacgtcttc gggtgcgccg gcttcgatca gctcagcccg agcctcgtcg acgttctcct
      721 gcctgcgaga ggtgatcacc acgcccttcg ccccgctctg ggcgaactcg agggcagtcg
      781 cctggccgat gccacggctc ccacccgtca cgagggcgac cttgcccgct atcgaaacat
      841 ccaattgctc tccctgtatc agtggccgcc gcgtttgacg ccgccgtact tcatcctcgt
      901 atcggacatt gccgcggcgc gttgctcctc gtcacggatg ccggcgtcga cgacttctcc
      961 gatgaacagc gtatgcgtac cgagatcatg ggattgcctg atttcgcact cgagccaggc
     1021 gatcgcctcg tcgaagatcg gggcgccggt cttacctgtg ctgacgggct tgccgttgag
     1081 ggagttgccc tcccgctcgg ccggcttcga gaacttgacg aacggacgag tgtcctcagc
     1141 cgaccacagg ttcacggtga acgacccgcc ctcggtgatg agccgatgag tcaccgcttt
     1201 gttgtcgacg ccgattccga tcagaaccgg ctccatggag agttgtgtta tccaactggt
     1261 cgtcatcccg ttccactcct ctccggcagc cgagccgacc agggcgagcg cgttcgggat
     1321 cttccatgtc actttgttga tggtctcggg gtccactatc tcctctgcgt aaccaggcaa
     1381 tgcaccctaa tgttccggcc gtgcggatcc tcatagccac cacaggcgtg ttgcccgcgg
     1441 cccccactgc tgacttctgc cgccagctca tcggtgatca gacttcggtc accgtcatga
     1501 ccgtgatcag ggtcccccgc tccttcctcg aatcgttggg agacgagatg cgccgctctt
     1561 tcctcgactc ggcggacaac ggtgcgagcg aggaggagaa agctgcggcc tatctcgaag
     1621 agcgcggccg gcgtgcggta gacccgatct gtgccgccct ggctgccgaa ggcatcgaat
     1681 gcgacatccg gttcgtcgag ggtgacgacc ccgcagaggc aatcatctcg acggcggaga
     1741 aagtaggcgc cgagatggtg gtgatgggcg caacacgccg tcttttcacc gaggaggcgt
     1801 ggcgaagcgt ctcggcccgg gtgatggtca actcccacct ccccgtcctg ttggtccccg
     1861 gcgccaggat cgaagacacc atggagatgc cggtcatcga gctctagtga gaccagcagc
     1921 gaataacggg cgatcctgtc atgttggctg acgcatgagc aactggaacg acggaatagt
     1981 cgacgagttc aggtcgaaca gcggagaggt gggcggcata ttcgcaggca agccgttgct
     2041 cctcctccat cacagggggg cccgtaccgg cagagagcga gtcagcccgc tcatgtatca
     2101 gcgagttggc gactcgtatg cagtctttgc gtcgaaaggc ggtgccgaga ccaaccccga
     2161 ctggtatcac aatctccggg cccaccccga agtcgaaatc gaggtcggag agttcacact
     2221 cgacgtgcgg gcacgggtgg ccaggggcga agagcaggac acgatctggg agagacagaa
     2281 gcgggaattc ccgcaattcg ccgggtatga ggagaagacg tcccgcgaac agataccggt
     2341 catagtgctc gatccggcct agtgccggcc atcaccggtt caggaccagg actcgggtag
     2401 ctctgaggac tctttctcgg cctggatgcg ggcgacatat gccgccgcct cgctgcgccg
     2461 gctcattccc atctttgcca ggatgttcga cacgtagttc ttgacggtct tctccgccag
     2521 gaacatctcc tcggcgatct gcttgttggt catcccaccg gcgatgtgct cgacgatggt
     2581 gcgttcctgg gcagtcagtt ggccgagaag gggatcctcg gagtcgccct ggcgaagtct
     2641 ggtgaacagc cgatcggtca tctcggggtc gagtagcgac tcacccgccc cgacacgccg
     2701 tatgtcactc acgagctcct cggtcttgat ccgcttgagg acgtaacccg aggctccggc
     2761 catgatcgcc gccatcaaag cctcctcgtc ggcgaacgac gtgagcatga ggaccttgac
     2821 atcggggaag cgctcccgaa tcgttcggca ggcctccaca ccgctcccgt cgggaaggcg
     2881 gacatcgagc acgacgacat cgggctcatc gaatccaacc cggcggacac cctcgtcgac
     2941 cgaacccgct tcgccgacga ccgtcatgtc ctcctgagag tcgatcagag ccttgagccc
     3001 tgccctgacc acctcgtggt catcgatcag agatacgcgc aacgtcacgg ttcatacggt
     3061 aatggcctgt aacgtcgact catggtcgat cgcgtggctg tcactcgcct cagcccccta
     3121 atcgaggaag ctgccgcggt cggtgggacc gccgacctcg accggctcct caggactctg
     3181 ctgtcggagg cgaagactgc gacgggagcg cgttatgcgg cactgggcgt catcggagat
     3241 catggagtgc tgtccgactt catctacgac ggcatcggcg acgatgtggc gcaacagatc
     3301 ggtccccctc cgagcgggcg tggcgtcctc ggcactttga tccgagaccg acagtccctg
     3361 atcctcgatg tcatcagcgg ccatcctgac tcggtcgggt ttcccgagca ccacccgccc
     3421 atggcgacct ttctaggcgt acccatcagc gcgggagacg agagtttcgg caatctgtac
     3481 ctcacggaga aggacggggg tttcaccgag gacgatctga ttgcggtcaa agcgctgagc
     3541 gccatcgcgg gcacggccat ccagagtgct cgtctgcaac gccgattgca gcagatggcc
     3601 gtggtcgagg atcggcagcg gatcgcccgc gacctccacg actcggtcat ccaggacttg
     3661 ttcgccgtag gactgggtct ccaggcgctc tcgttggtcg tggacgaggc agttgcagcc
     3721 agcatcgact cggcgatcga cacactcgac tcctcagttg ccacgctgcg caggtacatc
     3781 ttcgagctca aagagacgcc ccggcccgcc cagcggctcg atgagcgagt cagggatgtg
     3841 gtcgagcgca tgtccaaggc ctactccacc cgggtcgagc tcaccgtcga agacgtccat
     3901 cacgggccat gggtggagga cgccgttctc ctcaccaccg aggccctgtc gaatgctctg
     3961 cgccatggga gggcagacct catcgagatc tcacttcggg tggaggatga tcaccaggtc
     4021 atcgaggtga ccgacgacgg caacggattc gacctcggcg acgtcagcac ggggatgggg
     4081 ttgagcagca tgaaggcgcg cgccagtgcc accgggggat gggcggacat cgagagcaca
     4141 ccggaacgag gaacccgggt cactgcccga cttccgatca gacccgctga ccctccgggc
     4201 tgagagcctc ggcgagccgg tccatccagc ttgtgacggt tgcctcggcg acgcgcccga
     4261 ggacggcacg atctgcaatg cgacccaagg ctcccaaagg gggctgatat gtgccgcgca
     4321 gagtcagttt ggtggcacct ttgccggcgt tggcgaggat gaggtctgca gtcagctccg
     4381 ggaagaggag ccgcgccccg acagcactcc atcgcaccgg atacgcgagg ccgtaggtgt
     4441 ggatctcggc cacaccgatc tcgagggtga ccaccttggc aagggtctcg gtcggcccca
     4501 ccttcgcctg cagtgcctca ccctggcga
//
LOCUS       DASUTA010000321         5621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1909539, whole genome shotgun sequence.
ACCESSION   DASUTA010000321 DASUTA010000000
VERSION     DASUTA010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1909539"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="VFZ80_06405"
     CDS             complement(<1..1177)
                     /locus_tag="VFZ80_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6287098.1"
                     /translation="MGLQGKATAEWFDRASANLINGVSSQFRYWGDDDTLVIDRGEGG
                     HVFDMDGKRYIDYQCGFGPIILGHAEEAVVAAVAEAASEGTTFAMSQKREVEAAERVM
                     EALAWADGMRFTNTGTEATMHAIRLARGFTGRDLVLKFEGQYHGMHDYVLFSTAGSSP
                     DGLGSRFRPIPTQSSSGIPDSIRSYIRTLPFNDLELAQRLFADEGRRIAAVIVEPMLG
                     NAFGLMPVDGFLQGIRDLCDDAGAVLIFDEVKTGFRIATGGANEYFGVTPDIGTYAKA
                     MGNGFPVAAIATRGDLVQGWKQGGIAQAGTYSGNGIAAAAANATVTQLLTGEPLARVE
                     KVGTALMEGLGRVLADKGVEGEVTGHPSMFSIYLGEGEPRDFRDSAGHDQDLYEDVCM
                     "
     gene            1322..2758
                     /locus_tag="VFZ80_06410"
     CDS             1322..2758
                     /locus_tag="VFZ80_06410"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003953440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6287099.1"
                     /translation="MVQDLKLFIAGEWTEGSGDDHYEVSSPVTGEHIYNVPKASQADI
                     DRAVAAAREATEEMRHWTAFERAELCLRIYDLWQEQIESVARILTTEQGKPLAEESID
                     DIAESADYFQIAAEDVKRLSGEVIPTTERTRRMFTVHRPVGVWAAITPWNFPVMIPME
                     YVGPGLATGNSLIVKPPEFTCWALLEIAKVFEEAGVPKGAISVIPGAGEIGEALVTHP
                     GIDAVGFTGSSSTGARIVSQMGLKRSIMEMSGNGPTVVTADADIQKAAEAAVYGAYYN
                     AGQVCVATERLIVLDEVHDEFVEATMKAAEVVRLGDPFDDNTNMGPLNNTATAEKMDR
                     HVADAVERGAEILVGGKRASGFPTDLYYEFTVIDRVPEDSAVSQEESFGPVLPILSAR
                     DDEDAVAVANRTRLGLQAAVFTNDLSKAFYYADRIRSGTVIVNDSTDTWETFQPFGGA
                     AGTDTGWGRQRIDDFTDLQTIVFSLRDE"
     gene            complement(2761..4218)
                     /locus_tag="VFZ80_06415"
     CDS             complement(2761..4218)
                     /locus_tag="VFZ80_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6287100.1"
                     /translation="MERKPRVLVTGATGYIGGRLVPRLLESGAEVVCLARTPAKLDGV
                     WWRDEVEVREGDLLDPASLAGALEECDYAFYLVHSMEQKGADFSDRDRRAAKNFAAAA
                     DDSGLKRIIYLGGLGEGDLSSHLASRQEVGQILAEGKVPVTELRAAVIIGSGSVSFEM
                     LRYLTEVLPVMITPKWVRTRCQPLAIADLLEILVGALGDTGPSVIREIGGPQALTYEQ
                     MMRTYAEVAGLPRRWIIAVPALTPKLSSLWIGLVTPLPTAVARPLVDSLRVEVVVKDN
                     TYAEEKTSGLTSYRDAVSGALHGASELEVPTRWSGVSGRPALPYPTDPDWSGGTVETD
                     VQTVPSRASAQDVFGVVTSLGGDVGYLTMNWAWGIRGLIDSLVGGVGLRRGRRHPRDL
                     RTGDSVDFWRVVRVVPNSVLELYAEMRLPGEAWLSFEVDGTETGSVLTQTALFRPRGL
                     LGRLYWWAMFPFHVLIFGRMARQIARRAEASRGTV"
     gene            complement(4275..4502)
                     /locus_tag="VFZ80_06420"
     CDS             complement(4275..4502)
                     /locus_tag="VFZ80_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287101.1"
                     /translation="MAEHSEGDTSTDSRMGKAVARGVLIGFPVCLIGLTIAIWLITDL
                     DIFDSFATALLPGILLGGFAGGFAGVAATMD"
     gene            4577..5581
                     /gene="speB"
                     /locus_tag="VFZ80_06425"
     CDS             4577..5581
                     /gene="speB"
                     /locus_tag="VFZ80_06425"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX6287102.1"
                     /translation="MFTPYEERQSQIPFASAEYISFASRNSFLGCPAVTFDELGDGDV
                     VIVGAPFDWGTTYRPGARFGPSAIRNADYGAMDGYRPHLPTGLDPFEVLGVVDIGDIY
                     VQPGLLEGSIERIADVVEGIARKGKVPVILGGDHTITWPDALGVARVHGFGEVALIHF
                     DAHADTGFTQNGGLIGHGTPMRRLIESGAIPGHRFVQIGLRGYWPEPEVMEWMREHKM
                     RSFLMNEIVDRGLDEVVDEAVRYSLEGGAKGVFISVDIDVVDPGLAPGTGTPEPGGLN
                     SREILDTVRRLSKDLNVLGADIVEVSPPYDGPGEQTAYLANRVVLEVLNGMSERKTGA
                     "
BASE COUNT         1060 a         1857 c         1747 g          957 t
ORIGIN      
        1 tcatgcagac gtcctcgtag aggtcctggt catgcccggc gctgtcccgg aaatctctcg
       61 gctcgccctc ccctaggtag atcgagaaca tcgagggatg tccggtcacc tcaccttcga
      121 ctcccttgtc ggcgaggacg cggcccagcc cctccatgag cgccgtcccc accttctcca
      181 cgcgagccag cggctcacct gtcagcagct gggtcacggt cgcgttggca gcggcggcgg
      241 cgatcccatt gccggagtag gtgccggcct gggcgatgcc gccctgtttc caaccctgga
      301 ccaggtctcc cctggtggcg atcgccgcca ccggaaaccc gttgcccatc gcttttgcgt
      361 acgtcccgat gtcgggcgtc actccgaagt actcgtttgc gccgccggtg gcgatgcgaa
      421 aaccggtctt cacctcgtcg aagatcagaa ccgcaccggc gtcgtcgcac agatctcgga
      481 tgccctgcaa gaacccatcg accggcatca acccgaaggc attccccagc atgggctcga
      541 cgatcacggc cgcaatacgg cggccttcat ctgcgaagag acgttgagcc agctcgaggt
      601 cgttgaacgg gagtgttcgg atgtagctcc gaatcgagtc cgggataccc gagctcgact
      661 gcgtcgggat cgggcggaac cgtgagccga gaccatcggg agacgacccg gcggtggaga
      721 agagcacgta gtcgtgcatg ccgtggtact ggccctcgaa cttcaacacg aggtccctcc
      781 cggtgaaccc gcgcgccagt cgaatggcgt gcatcgtggc ttcggtcccc gtgttggtga
      841 aacgcatccc gtcggcccag gcaagcgctt ccatgaccct ctcggctgcc tcgacctcac
      901 gcttctgtga catggcgaag gtggttccct cggaggccgc ctcggcaacg gcggcgacaa
      961 cggcttcttc ggcgtgaccc aggatgatcg gcccaaaccc gcactggtag tcgatgtagc
     1021 gcttgccgtc catgtcgaag acatggccgc cctcgccgcg gtcgatgacg agggtgtcgt
     1081 cgtctcccca atagcgaaac tggctggaga ctccgttgat caagttcgcc gaagcgcggt
     1141 cgaaccactc agccgtcgcc tttccctgca gacccatgag actccttgga cgaagtgggt
     1201 caggatacag agggttgcct catttgcttt cctcccttcg gcggatcgga ggtggtctca
     1261 cagttgcatg tgcgaaacgg tctcagggct tcatgagtgc cgtggtgact acctttgagt
     1321 catggttcag gatctgaaac tcttcatcgc cggcgaatgg acagaaggca gcggtgacga
     1381 ccactacgag gtctcgtcgc cggtcaccgg cgagcacatc tacaacgtgc cgaaagcatc
     1441 gcaggccgac atcgaccggg cggtggccgc ggcccgggag gcaaccgagg aaatgcgcca
     1501 ctggaccgcg ttcgagagag ccgaactctg tctcaggatc tacgacctct ggcaggaaca
     1561 gatcgaatcc gtcgcacgca tcctcaccac ggagcagggt aagccactgg ccgaggagtc
     1621 gatcgacgac atcgcggaat cagccgatta cttccagatc gccgccgaag acgtgaagag
     1681 actctccggt gaagtgatac cgaccacaga gcgcacgaga aggatgttca ccgttcacag
     1741 gcccgttggc gtgtgggccg cgatcacccc gtggaacttc cctgtgatga tcccgatgga
     1801 gtacgtcggg cccggtctcg ccaccgggaa ctcgttgatc gtgaagccgc ccgaattcac
     1861 atgctgggct ctgctcgaga tagccaaggt cttcgaggaa gcgggcgtgc ccaagggtgc
     1921 catctcggtg atcccgggag ccggggagat cggcgaggcc ctggtcactc atcccgggat
     1981 cgatgccgtc gggttcaccg gttcctcgtc gacaggtgct cgcatcgtct cccagatggg
     2041 tctgaagcgg tcgatcatgg agatgtcggg caatgggccc acggtggtga cggccgatgc
     2101 cgacatccag aaggcggccg aggcagccgt gtacggcgcc tactacaacg ccggtcaggt
     2161 ctgtgtcgcc accgagcggt tgatcgtcct cgacgaagtc cacgacgagt tcgtcgaggc
     2221 caccatgaaa gcggccgagg tggtgaggct gggtgacccc ttcgacgaca acaccaacat
     2281 ggggccgctc aacaacaccg caactgcgga gaagatggac cggcatgtgg cagacgccgt
     2341 cgagcggggc gccgagatcc tcgtcggggg caagagggcc agtggcttcc ccaccgacct
     2401 ctactacgag ttcacggtca tcgacagggt gccggaggat tcggccgtgt cccaggagga
     2461 gtcgttcggg ccggtgctcc ccatcctctc cgcacgagac gacgaggacg ccgtcgccgt
     2521 ggcgaatcga acccgtctcg gcctgcaggc ggcggttttc accaacgacc tgagcaaggc
     2581 cttctactac gccgatcgaa tcaggtcggg caccgtgatc gtcaacgact ccaccgacac
     2641 ctgggagacc ttccaaccgt tcggcggagc agccgggacc gacaccgggt ggggccgaca
     2701 gcggatcgac gacttcaccg atctgcagac gatcgtcttc tcactgcgcg acgagtagac
     2761 ctagacagtc ccccgggacg cctccgccct gcgggcgatc tggcgcgcca tgcgcccgaa
     2821 gatcaacacg tggaacggga acatcgccca ccaatagagc cggcccaaca gcccccgcgg
     2881 ccggaagagg gcggtctgcg tgagcacgct cccggtctcg gtcccgtcta cctcgaaaga
     2941 gagccaggcc tcaccgggga gtctcatctc ggcgtacagc tcgagcactg agttgggcac
     3001 cacgcggacc acacgccaga agtcgactga atcgccggtc ctcaagtctc tcgggtgccg
     3061 gcggccccgc cgcagaccca cgcctccgac gagagaatcg atcagaccgc gtatgcccca
     3121 cgcccagttc atcgtcaagt agccgacatc accgccgaga ctggtgacga caccgaacac
     3181 gtcctgggcc gatgcccggg acgggaccgt ctggacgtcg gtctcgacgg tccccccgga
     3241 ccagtcggga tcggtgggat agggaagcgc cggccgccca ctcaccccag accaccgggt
     3301 ggggacttcc aactcgctgg ctccatgaag agcgccggag acggcatccc gataggaagt
     3361 gaggcccgac gtcttctcct ccgcatacgt gttgtccttc acaaccacct cgacacggag
     3421 actgtcgacc aacggccttg ccaccgcagt gggaagtggt gtgaccagcc cgatccacag
     3481 gctggacagc ttcggagtga gagccgggac tgcgatgatc cagcgtcggg gcagaccggc
     3541 cacctcggcg tatgtgcgca tcatctgttc gtaggtgaga gcttgggggc caccgatctc
     3601 cctgatcacc gacgggccgg tgtcgccgag ggccccgacg agtatctcga ggaggtcggc
     3661 gatggcgaga ggttgacacc tggtccgcac ccatttgggc gtgatcatca cggggaggac
     3721 ctcagtgaga tagcgaagca tttcgaaaga gaccgagccc gacccgatga tgactgcggc
     3781 tctcagctcg gtgaccggca ccttcccctc ggccaggatc tgcccgactt cttgccggct
     3841 ggcaaggtgc gagctgaggt cgccttcgcc caggcccccg agatagatga tccgcttcag
     3901 gcccgaatcg tcggccgccg cagcgaagtt cttggcggcc ctccggtctc gatcgctgaa
     3961 gtcggctcct ttctgttcca tcgaatggac cagatagaag gcatagtcac attcttcgag
     4021 agcaccggcg agcgacgccg ggtcgagcag atccccctcc cggacttcca cctcgtcgcg
     4081 ccaccagacg ccgtccaatt tggcaggcgt cctcgccaga cagacgacct cggcccccga
     4141 ttccagcagc cggggcacca atcgccctcc tatgtatccg gtggcacctg tgaccagaac
     4201 tcgaggtttt ctctccatca ccacggattc gtagctgagt cacgcacgga tgagcgaaga
     4261 cgacgacgga tggatcagtc catcgtggcg gcgacaccgg caaacccgcc ggcgaagccc
     4321 ccgaggagaa tccctgggag gagagccgtg gcaaacgagt cgaagatgtc gagatcggtg
     4381 atcagccaga tggcgatcgt caatccgatc agacacaccg gaaagccaat aaggacgcca
     4441 cgggcaacag ccttgcccat gcgggagtcc gttgaggtgt cgccttcgct gtgctctgcc
     4501 atcgggcaat catgccagat cgacttctcg tgtgacaccc ccgcatatcc accggtccag
     4561 ttagcgtacg acgtcgatgt tcacgccata cgaggagcgc cagtcacaga ttccgttcgc
     4621 ttcggcggag tacatctcat tcgcttcccg taactcgttc ctcggatgtc ccgccgtcac
     4681 tttcgacgag ctcggcgacg gagatgtcgt gatcgtgggt gccccattcg actggggcac
     4741 cacctacagg cccggtgccc gcttcgggcc ctccgccatc cgaaacgccg actacggggc
     4801 gatggacggc taccggcctc atctccccac aggtctcgac cccttcgagg tcctcggcgt
     4861 cgtcgatatc ggagacatct acgtccaacc cggcctgctc gaagggtcga tcgagcgcat
     4921 cgccgacgtc gtcgagggga tagctaggaa aggcaaagtc cccgtcatcc tcggtggcga
     4981 ccacacgatc acctggcccg acgcgctcgg tgtggcgcgt gtacacgggt tcggtgaggt
     5041 cgcactcatc catttcgacg cccacgccga caccggcttc acccagaacg gggggctgat
     5101 cggccacggg accccgatgc gccgtctcat cgagtcaggt gcgatccccg gccatcgctt
     5161 cgttcagata ggcctgcgcg gctactggcc cgagcccgaa gtcatggagt ggatgcggga
     5221 gcacaagatg cgctcgttcc tcatgaacga gatcgtcgac aggggcctcg acgaagttgt
     5281 cgacgaagcg gtccgctact cactcgaggg cggggcgaag ggcgtgttca tctcggtgga
     5341 tatcgacgtg gtcgatccgg ggctggcgcc cggaaccgga acccccgagc ccggcggtct
     5401 caactcgcgg gagatcctcg acaccgtgcg gagactctcc aaggatctga acgtcctagg
     5461 cgccgacatc gtcgaggtgt cccctcccta cgacgggccc ggtgagcaga ccgcctatct
     5521 cgccaaccgc gtggtcctcg aggtcctcaa cggcatgtca gagcggaaga cgggggccta
     5581 gcgcccctca gcgaacccgg ggttcctcgg gccggatatg a
//
LOCUS       DASUTA010000322         5099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1391435, whole genome shotgun sequence.
ACCESSION   DASUTA010000322 DASUTA010000000
VERSION     DASUTA010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391435"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..819)
                     /locus_tag="VFZ80_06430"
     CDS             complement(52..819)
                     /locus_tag="VFZ80_06430"
                     /inference="COORDINATES: protein motif:HMM:NF040399.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6390 family protein"
                     /protein_id="HEX6287103.1"
                     /translation="MEETGSEPSVISGPELFFRYAQPPNRLGYCGPNRGSEIAVAASG
                     DSLPFEEAVALALAFDGAWPYLELIASLTGHDPLSRQVVEAYWMGTELLDGIDLHHWG
                     HSISDRFKTRAGSRWPAVLEALNRGGSPTHAFHVFCVYPWVGLLRSGFSAPAVEVLDR
                     CRIAVGEVMTADGELAVVRRRPLIWEDDSLRPGAPIAEPFRATSVVHPGDVVSLHWDY
                     ICQRLDPRQKRRLIASHDRHLQIANDELRRARMEPAH"
     gene            complement(789..1718)
                     /locus_tag="VFZ80_06435"
     CDS             complement(789..1718)
                     /locus_tag="VFZ80_06435"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6287104.1"
                     /translation="MTRDLSTRRKGILLAAGTAVISGFAVFLNGYAVRAWAEVSDATT
                     YTTAKNLLAGLAIGLFGLGMSKAASPRRPSIPAGTKSRVMLVAIAVIGGSVPFALFFE
                     GLARASSAQAAIIHKTLVVWVTLLALVFLRERIGWPHVGAIGLLVWGQTVLAGGLGAL
                     SWGSGETLILVATLLWSVEVVVAKRLLPEVSYATVAKARMIGGAAVLVAWTLVRGADV
                     EWAALTSSRMIWVVVAAVFLSGYVLTWFAALSMAPAVDVTAVLVGGALITAFLQSTLE
                     GDPLPDPTGALLVLAGMALAAAASWRRPGASRL"
     gene            1805..2914
                     /locus_tag="VFZ80_06440"
     CDS             1805..2914
                     /locus_tag="VFZ80_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6287105.1"
                     /translation="MVTLVIDTDGLSSLFDTLEEEGYTLIGPTVADQAIVYDTIHSPD
                     DLPRGVTDVQEGGRYRLEKREDDARFGYNVGPTSWKRYLFPPRTELLRARRRDGRLVF
                     ETSEEIPPRYAFIGVRPCDLAAISIQDRVFLGSGRVDRTYASRRREVFMVAFNCGQAG
                     ATCFCASMGTGPRATDGYDLVITELNEAEGPRYVVETGSARGETVAKRLPGGLATEDD
                     LAGVASAVARAESEMGRRLDTNGIRDLLVENPDHPRWDEVAQRCLTCGNCTLACPTCF
                     CSTTEDAVTLDGTAVRSRRWDSCFGLDFSGLHGHPVRSAAKSRYRQWMTHKLATWHDQ
                     FGTSGCVGCGRCITWCPVGIDITAEVAAIRETVRA"
     gene            2911..3369
                     /locus_tag="VFZ80_06445"
     CDS             2911..3369
                     /locus_tag="VFZ80_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6287106.1"
                     /translation="MRLESLREQTPFLAGLSNQALDLLEPVTADAEFAAGESIFEEDG
                     PADTFYVIRSGKVALEIELPSRGTSVVETLGPGSLLGVSWPFPPARWNWRARAVDTTD
                     TIAFDASAVRRLCDANQELAAHVYRSVAAEAVRRLHATRIRLLDLYGSDR"
     gene            3366..4214
                     /locus_tag="VFZ80_06450"
     CDS             3366..4214
                     /locus_tag="VFZ80_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018877004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEX6287107.1"
                     /translation="MTTQLMAHPDEAMLPRSFRLVSKSHETADTVTLIFEDPDGMPAA
                     FEPGQFMMIYVFGVGEIPISIAGNPHMPHDLVHTVRAVGAVTDVLCALEPGDMVGMRG
                     PFGTSWPLEQALGRDLVIVTGGIGLAPLRPAVLEALNRNADFNSIALVYGARTPSDLL
                     YRDDLLGWVSTPEMRVEVTVDRAGTDWWGDVGLVTGLIPRLAFEPASTAAFVCGPEVM
                     MRVVARDLRDRGVPADHIFLSIERNMKCAIGFCGHCQYGSDFICKTGPVGSYERYEQR
                     LRVSEI"
     gene            4211..4984
                     /locus_tag="VFZ80_06455"
     CDS             4211..4984
                     /locus_tag="VFZ80_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017290643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX6287108.1"
                     /translation="MNKRPKLAVWKFASCDGCQLSLLDCEEELLAIAEAIEIAYFPEA
                     SRAVVEGPYDLSLVEGSVTTSHDAERIQEIRAQSGALVTIGACATAGGIQALRNWADV
                     SAFTSIVYAEPSFIDTLETSTPIGAHVDVDLELRGCPISKYQLLEVVDAFLHERRPNV
                     PRHSVCVECKARGTPCIMVRDGIPCLGPVTQAGCGAICPAYDRGCYGCFGPAETPNLD
                     ALAAWWEGLGVEKPDLVRALRTFNAYAPEFREASERYAQ"
BASE COUNT          938 a         1673 c         1636 g          852 t
ORIGIN      
        1 cagctgtagc tcagctcgga gtgcggctca tcagagacga ccgtcaggct ctcagtgagc
       61 gggctccatt cgggcgcgac gcagctcgtc gttcgcgatt tggagatgtc ggtcgtggga
      121 agcaatcagc cttcgtttct ggcgcgggtc gaggcgctgg cagatgtagt cccaatgcag
      181 agacaccaca tctccagggt gaacaaccga cgtggcacgg aaaggttccg caatcggtgc
      241 cccaggacgc aggctgtcgt cttcccagat gagggggcga cgtctcacca cggccagctc
      301 cccgtcagcc gtcatcacct cgccgacagc gatgcggcag cggtccagca cctcgaccgc
      361 cggagcggag aaacccgagc ggagaagacc cacccatggg tatacgcaga agacgtggaa
      421 ggcgtgagtg ggcgagcctc cccggttcaa tgcttccagc accgccggcc agcgactgcc
      481 ggctcgcgtc ttgaatcgat ctgagatcga atgtccccaa tgatggagat ctatcccgtc
      541 caacagctcg gtgcccatcc aataggcctc gacgacctgt cgggacaggg gatcgtggcc
      601 ggtcaggctg gcgatgagct cgaggtaggg ccacgctccg tcgaaggcga gagcgagtgc
      661 gacggcctcc tcgaacggaa ggctgtcccc ggatgcggcc acggcgatct cggagcctcg
      721 gttcgggccg caatagccaa gacgattcgg cggctgggcg taacggaaga agagctcggg
      781 tccactgatc acagacggct cgctcccggt ctcctccacg atgcggccgc ggccagagcc
      841 atccctgcga gcacaagaag cgccccggtc gggtctggta gcggatcgcc ctcgagcgtc
      901 gactggagaa aggctgtgat caacgcccct cccaccagaa ctgcggtgac atcaacggct
      961 ggggccatcg acagggcggc gaaccaggtg agcacatatc cggagaggaa aacggcggcg
     1021 acaacaaccc agatcatccg ggatgacgtc aaggctgccc actcgacatc tgccccccgg
     1081 acgagggtcc acgccaccag gacagcagcc ccgccgatca tgcgcgcttt ggcgacagtg
     1141 gcataggaca cctctggaag cagacgcttg gctaccacca cctccacgga ccagagcagt
     1201 gtcgccacca ggatcagtgt ctcgccgctt ccccaggaga gggcgcccag tccaccggcg
     1261 aggaccgttt gaccccaaac gaggagaccg atggcgccga catgcggcca cccgatgcgc
     1321 tcgcgaagga acaccagcgc cagcagcgtc acccagacga ccagcgtctt gtggatgatc
     1381 gctgcctgtg ccgagctggc tcgggccaaa ccctcgaaga agagggcaaa gggcacgctt
     1441 cctccgatta cggcgatcgc cacgagcatc acccgcgatt tcgtaccggc cgggatcgac
     1501 ggtcgccgag gcgaagctgc tttggacatt ccgaggccaa agagcccaat ggccaacccg
     1561 gcgaggaggt tcttcgcggt ggtgtaggtc gtggcgtcgg acacctcggc ccacgcccgg
     1621 accgcatagc cgttcaagaa cactgcgaat ccgctgatga cagcggttcc ggccgcgagg
     1681 aggatgccct tgcgacgagt gctgagatct ctggtcataa tcccatcatc tctgggtcca
     1741 acacccgtca aacagggtcg ttggccccta aacacgggtc tcaccggccc atagcgtcag
     1801 cgtcgtggtc acactcgtca tcgacaccga cgggctctcc tccctgttcg acactctgga
     1861 agaggagggc tacaccctca ttggtccgac cgtcgccgac caggccatcg tgtacgacac
     1921 gatccactcc ccggacgatc ttccccgtgg ggtgaccgac gtccaggaag gcggcaggta
     1981 ccggctcgag aagcgggaag acgacgcccg cttcggatac aacgtgggcc ccacctcctg
     2041 gaagcgctac ctcttcccac cgcggaccga actactgcgc gcccgacgac gtgacgggcg
     2101 tctcgtcttc gagaccagcg aggagattcc gcccagatac gctttcatcg gagtccgccc
     2161 gtgtgatctg gcggcgatat ccattcagga tcgggtcttc ctgggatccg gaagagtgga
     2221 taggacctac gcgtcccggc gtcgcgaggt gttcatggtc gcgttcaact gcggccaggc
     2281 cggagccacc tgtttctgcg cctcgatggg aaccggtccg agggcgaccg acggttatga
     2341 cctcgttatc accgaattga acgaggccga agggccgcgg tatgtcgtgg agaccggaag
     2401 cgccaggggc gagaccgtcg ccaagaggct tcctggtggt ctcgccaccg aagacgacct
     2461 cgccggcgtg gcctcagccg tcgccagggc agaaagtgag atgggacgcc gcctcgacac
     2521 caacggaata agagatctcc tggtcgaaaa ccctgatcac cctcgttggg acgaggtggc
     2581 ccagcgctgt ctgacctgtg gcaactgcac actggcctgc ccgacctgtt tctgctcgac
     2641 cacggaggac gcggtcactc tcgacgggac cgccgtgcgt agtcgccgtt gggacagctg
     2701 cttcggcctc gacttctctg ggctccatgg tcacccggtg aggagcgcgg cgaagtcgcg
     2761 ctatcgccag tggatgaccc acaagctggc gacatggcat gaccagttcg gcacgtcggg
     2821 gtgtgtcgga tgtgggcggt gcatcacctg gtgcccggtg gggatcgaca tcaccgccga
     2881 agtagccgcg atcagagaga ccgtgcgggc atgagactcg aatccctgcg cgagcagacc
     2941 cccttcctcg ccggactctc caaccaggct ctcgatctgc tcgagcccgt gaccgccgat
     3001 gccgagttcg ccgcaggtga gtcgatcttc gaagaggacg gtccggccga cacgttctat
     3061 gtcatcaggt ccggaaaggt cgcactcgag atcgagcttc cgtcccgggg aacctcggtc
     3121 gttgagacgc tcgggccggg cagcctgctc ggagtcagct ggcctttccc gccggcccgg
     3181 tggaactggc gcgcccgagc cgtggacacc acggacacga tcgccttcga cgcgtcggca
     3241 gtccgccgtc tgtgcgacgc caaccaggag ctggccgcac acgtgtaccg gagtgtggcg
     3301 gccgaggcag ttcgccggct ccacgccacc aggatccggc tactcgacct ctacgggagt
     3361 gaccgatgac cactcagctc atggctcacc ccgacgaggc aatgctgccc cgttcgttcc
     3421 ggctggtctc caagtctcac gaaacggccg acacggtgac gctgatcttc gaggaccccg
     3481 atggcatgcc agccgcattc gaacccgggc agttcatgat gatctacgtc tttggagtgg
     3541 gtgagattcc catctcgatc gccggtaatc ctcacatgcc ccatgatctg gtgcacacgg
     3601 ttcgtgcggt gggtgcggtg accgacgtct tgtgtgccct ggagcccgga gacatggtgg
     3661 gcatgcgagg ccccttcggg acgtcatggc cgctggagca ggccctgggg cgtgacctgg
     3721 tgatcgtcac cggcggtatc gggttggcgc ctcttcgacc cgcggtgctc gaggccctca
     3781 accgcaacgc cgacttcaac agcattgccc tcgtctatgg ggcgcgtacc ccgagcgacc
     3841 tcctctaccg cgacgacctc ctcgggtggg tttcgactcc ggagatgcgt gtggaggtga
     3901 ctgtcgaccg cgccggcacg gactggtggg gtgatgtggg gctggtgacg gggctgattc
     3961 cccgcctcgc cttcgaaccc gcctcgacgg cagcgttcgt ttgtggcccg gaggtgatga
     4021 tgcgggtcgt cgccagggac ctgcgtgacc ggggggtgcc ggcggatcac atcttcctga
     4081 gcatcgaacg caacatgaag tgcgccatcg gtttctgcgg tcactgtcag tacgggtccg
     4141 acttcatctg caagacaggg ccggtcggat cgtacgagcg ctacgaacag cggctgagag
     4201 tgagcgagat atgaacaagc gacccaagct cgccgtgtgg aagttcgcct cttgcgacgg
     4261 ctgccagctc agcctgctcg actgcgaaga agagctgctg gccatcgccg aggccatcga
     4321 gatcgcctac ttccccgaag ccagccgtgc cgtcgtcgaa gggccctacg acctctccct
     4381 cgtcgaggga tcggtcacca cctcccacga cgccgagcgg atccaggaga tcagggcaca
     4441 gtcgggtgcc ctggtcacga ttggcgcctg cgccacggcg ggcggaatcc aggccctgcg
     4501 caactgggcc gatgtttcgg cgttcacctc gatcgtctac gcggagccct ccttcatcga
     4561 cacgctcgag acgtcgacgc ccatcggcgc tcacgtcgat gtcgacctcg aactgcgggg
     4621 atgcccgatc tccaagtatc agctcctcga ggtggtagac gccttcctcc acgagcggag
     4681 gcccaacgtt ccccggcaca gcgtgtgtgt cgaatgcaag gcgcggggaa cgccctgcat
     4741 catggtgcgc gacggaatcc catgtctcgg tccggtgacc caggccggct gtggggcgat
     4801 ttgccctgcc tacgaccgcg gctgctacgg ctgcttcggg ccggccgaga cgcccaacct
     4861 cgatgccctc gccgcatggt gggaagggct aggggtggag aagccagacc tcgtgcgggc
     4921 gctgcgcacc ttcaacgcct acgcacccga gttcagagag gccagcgagc gctatgcaca
     4981 atgaacgtga gatcgacgtc tcctatctcg cccgggtcga gggcgagggg gccatgtacc
     5041 tcaagctcgt cgacgacgag gtggccgacc tgaaattcaa gatcttcgag ccaccccga
//
LOCUS       DASUTA010000323         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1780038, whole genome shotgun sequence.
ACCESSION   DASUTA010000323 DASUTA010000000
VERSION     DASUTA010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780038"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2111
                     /locus_tag="VFZ80_06460"
     CDS             <1..>2111
                     /locus_tag="VFZ80_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX6287109.1"
                     /translation="SGRGKLVVTATGARTQLGAIAEMTGSLAETPSPLQRELKRLSVA
                     VSVIAVGVGVLLFGLALVLADMPVARGFVFALGMIVAFVPEGLLPTVTLSLAMATQRM
                     AERSALVKKLSSVETLGSTTVICTDKTGTLTANEMTVRSVTTSGRTYRFEGVGYEPVG
                     DVSPSPDDSLRVLLSSAALASNARLTRDIDGRWSLVGDPTEGAILTAALKAGIDLDDL
                     STDNPRLDEIPFDPNRKRMSTVNRVDGVLMMCTKGAPAELLERCDSVASRGGPVPLTE
                     LDRKAALEEMDRLAVEGLRVLGVARRRLAELPDLDEGVESGMEFLGLIAMQDPPRPEV
                     QAAIAKCRSAGVRIVMITGDYGMTAEAVGRRIGMIEPGQVRIVNGTDLDALTDAELES
                     ILDHEVVFARVAPDHKLRVVAALQARGEVVAVTGDGVNDAPALKKADIGVAMGVSGTD
                     VAREAADIILLDDNFASIVAAVEEGRAVYDNIKKFTTYILTSNTPEAVPFAVFAFSGG
                     RIPLALDVMHILAIDLGTDLAPALALGAEKPEPGIMERPPRPRDGHLIDRPLLTRAYL
                     WLGPLQAAFVMAAFFGAYRLVGIDGWLDLPSEGPIYASATAMALAAVVATQIGNLFAQ
                     RSDRVSLFRIGLGGNRLLWWGILSELIVIALIVYVPFLQNVIGTAPFPAVGWAWLLLG
                     LPLLPMADEVRKLVVRSTRRR"
BASE COUNT          354 a          628 c          716 g          413 t
ORIGIN      
        1 tcgggacggg gaaagctggt ggtcaccgcc actggagctc gcacccagtt gggggctatt
       61 gccgagatga ccggttctct ggcggagacc ccgagcccgc tgcagaggga gctcaaacgg
      121 ctcagtgtcg cggtcagcgt catcgccgtc ggtgtcggcg tgttgttgtt cgggttggcg
      181 ctcgtcctgg cagacatgcc ggtggcgaga ggattcgtct ttgccctggg tatgatcgtc
      241 gccttcgtgc ccgagggcct actgccaacg gtgacgcttt cgttggcgat ggcaacgcag
      301 cgcatggcgg agagaagcgc gttggtgaag aagctgtcgt ccgttgagac actaggtagc
      361 acgactgtca tctgcacgga caagaccggc accctgaccg ccaacgagat gaccgttcga
      421 tcggtgacga cttctggccg gacctatcgg ttcgagggcg tgggatatga gcctgtcgga
      481 gatgtgagcc catcgccaga cgactcgttg cgagtgttgc tgagttcggc cgctctcgcc
      541 tccaacgccc ggctgaccag ggacatcgat gggcgatggt cgttggtggg cgaccccacc
      601 gagggggcga tcttgaccgc ggcgctcaag gctgggatcg acttggacga cctctcgact
      661 gacaaccccc gcctcgatga gatcccgttc gacccgaatc ggaaacggat gagcacggtg
      721 aatcgtgtgg acggcgtact gatgatgtgc accaagggtg ctcccgccga gctactggag
      781 cgatgcgact ccgtggcttc ccgaggggga ccggtgccct tgaccgagct cgatcgcaaa
      841 gctgccctcg aggagatgga cagactcgcc gtcgagggtc tgagggttct gggagtggcc
      901 cggcgacgac tggccgagct gcccgacctt gacgagggcg tcgaatccgg gatggagttc
      961 ctcggcttga ttgcgatgca agatccacct cgaccggagg tacaggcggc catcgccaaa
     1021 tgtcggtccg ccggggtccg catcgtgatg atcacaggcg actatgggat gactgccgag
     1081 gccgtcggca gacgcatagg catgatcgag ccgggacagg ttcggatcgt caatgggacc
     1141 gacttggacg cgttgaccga cgccgaactg gaatcgatat tggaccacga ggtggtcttt
     1201 gcccgcgtgg ccccggatca caaactgcgc gtggtcgccg ccctccaggc ccgaggtgag
     1261 gtcgtggccg tcaccggaga tggcgtgaac gatgcaccag ccttgaagaa ggcggatatc
     1321 ggcgtggcga tgggtgtgag cggaacggat gtggccagag aggcagccga catcattctc
     1381 ctcgatgaca acttcgcctc gatcgtggct gctgtcgagg agggccgcgc cgtctacgac
     1441 aacatcaaga agttcaccac ctacatcctc acgtcgaaca ctcctgaagc ggtgccgttc
     1501 gcggtgttcg ccttctctgg cggccgcatt ccgcttgctc tcgacgtcat gcacatcctc
     1561 gccatcgatc tcggcaccga cctggcaccc gctctggcgt tgggcgccga gaagcccgag
     1621 cccggcatca tggagcgccc ccctcgtcca cgagacgggc acctcataga tcggcccttg
     1681 ctgacacgcg catacttgtg gttggggccg cttcaggcgg ccttcgtcat ggcggcgttc
     1741 ttcggcgcct atcgattggt gggcatcgac ggttggctcg atcttccctc cgaggggccg
     1801 atctacgcgt cggcgactgc gatggccctg gcggcggtgg tggctaccca gatcgggaat
     1861 ctcttcgctc agcgatcgga tcgagtctcc ttgtttcgaa tcggcttggg tgggaaccgg
     1921 cttctctggt gggggatact ctcggagctg atcgtgatcg cgctcatcgt ctacgttccg
     1981 ttccttcaga atgtgatcgg gaccgcgccg ttcccggcgg tgggatgggc ctggctgctg
     2041 ctcggcctcc ctctgctgcc gatggcggac gaggtcagga agctcgtggt cagatcgacg
     2101 aggaggcgat a
//
LOCUS       DASUTA010000324         7210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_613964, whole genome shotgun sequence.
ACCESSION   DASUTA010000324 DASUTA010000000
VERSION     DASUTA010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613964"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1808)
                     /locus_tag="VFZ80_06465"
     CDS             complement(<1..1808)
                     /locus_tag="VFZ80_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6287110.1"
                     /translation="MCGVIADPDDRFCRNCGNPLGEDASSSEPTPVARAEEDLSRYLP
                     EELLAKMRSARDGHAMEGERRTVTMLFADVQGSTSSAEQLDPEDWADIMNGLFERLIA
                     PIYRYEGTLAQLRGDAVLAFFGAPIAHEDDPVRALRAGLEIVAATAGYSETVEKRWGV
                     PAHVRVGIHTGLVVVGAMGSDLRVEYTALGDAINVAARMEQTAEPDTVRVTGHTLSLT
                     NGVFEAEELGPIDVKGKSEPVPAYRVIRYLGRPSASGEGPIVGRADELAVLDEVRSVV
                     TSGSGKIVSVIADAGVGKTRLVEEFGRRTEEAVDRAYSYDQAGQVNWLRAASRSYDSS
                     RPYSTVADLLQRWWFSDGSEPAFDRVTEAVVAEGIDDPDVAAYLGFVADVPLPERAEG
                     FIDNLATQLLNSRAQAAVISYLQALVNRRPTIVVLEDLHWSDDLSLAVIEEMMGLAET
                     TPLGLVLVMRPYRDEPSWRLHEVAERTHPHRYIGLSLEPFDIEESSNLFDALIEGSPV
                     DSRIKEGILERSQGNPLYIEQMVRALSEIGSEEFDESQVPSSLRGLLTARLDRLGEEQ
                     RYLIQMASVLGSEFEKATLAALASSPNLESSLAHLLR"
     gene            2021..4978
                     /locus_tag="VFZ80_06470"
     CDS             2021..4978
                     /locus_tag="VFZ80_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX6287111.1"
                     /translation="MTEKGNARDRLLSSVGIRPDEVTLAGWMAILFMVTQASHAVGAN
                     AADTMFFLRYGVEDLPLMILLSGPAVMVAIVGHSAGLAWRGADTWLMIVMALSAAWVG
                     LLRAGILTGSAGIYPVIWISTQVLIFLTLTVMWNAAGSACTTRQAKRLFPVFATAAVA
                     GGVIGNLLVGPLTTLFGAENLLLVQGLLLVIATFLVVRVRSFFTSGSGEREPVRVEMT
                     RAFSSIRNSRLLGLAAGVVFLLFALFYLVVFPFSEIVTATFPAETEMAGFLGLFSSVA
                     TAATFLFSLLATGRLFSRLGLIVTLITVPVVYLLGFSTWLVAFGILTAVLFRGLQWVA
                     VNSVQTTAYSALFNVLSTRRRGPVLALMTAVPAQLGTMAAGAILLLGADGLSQAGLFW
                     VGALLAAAAVGLVAAMRPAYLAAVVEAVRRGLVTVWDSPHTGVVVTPVDRDVVRILES
                     HLSDHRPRARAIAAAGLGSLGDDSVLAGVEVLLDDDDPLVRSAAFTAVCQIEPAALEP
                     RLEAAIADESPEVRLNAVRYLDSIGERDLPVAALATVLDDPDPRVRAAGAWLAPEEAG
                     SPVAVDMVTSGRPDAVRAVFEEMLRHPGQTLGLRPRDFVADDDPAVRAAAAAAIAASG
                     SDLEAVLPSLDDRSVRVRRAAARALASSHEGRELLLDVLEGGSVIATEAALDAMTPVE
                     DLDQRFTAWAASEAQRASYLAGHRRLLETRLDSPQGRYLVRVLDSRVNRLVHWVLMAM
                     TTDATKEIMPLVARGVEADDPETNAQAIEALESVADRSVLSVLLPLLEQSGEGAGSDA
                     HEDTLRELSGDFDPWLSRLATLCLDRYASGNDDLASLGEMAGQELDTLDDMGRVLVLQ
                     QVPMFSGLDPEDLILVARFTKEARFDPNDPIYREGDPGTELLVIVEGSAVVSRTRAEA
                     RHLIDTYHAGEHVGELSLLTGGRRSADVEAGSDGLHGLVLGKSELMAILEERPAVALG
                     MLGTLASRLIEQT"
     gene            5018..6976
                     /locus_tag="VFZ80_06475"
     CDS             5018..6976
                     /locus_tag="VFZ80_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6287112.1"
                     /translation="MTLEVARDAIRSKDWSSAIQEFEDADEELSPDDLVLMADALWWS
                     ARPDEAVDAYQRAFEVFVRSDRKGRAAAVAATLSYLAMRRMSESVAMGWFSRAQRLLE
                     GEPEGPGHAWISLLEAAQSLFVMSDLDAVKEAADRTIDIARRQGVAGVEALALSFKGV
                     ATTNQGDWREGMRMIDEATVIATAQDGDLRSSSDVYCIMMGACSSLADYKRASEWTEQ
                     AERWMAVNSLGGFTGECQIHRAEFKRLHGDWFQAEHDARMACTELERFHLLNSLGYAN
                     YEIGEVRRRMGDLDAAEEAFTRAYEYGHPAQPGFSLLMMDRGDLDRAASSIEAALETT
                     RADGNRGDLLTRGHLLPAQIEIALARDDLDTAQRAVTQLEQVAETYDSPAWQAMLLTC
                     RGALSLAEGRSAEAVTALDRAWRLWQNVELPYETARSRELLGRARMAVGDETAAGLEL
                     RAAWSTYEKLGARLDLERLQESMGPAGAGTSSSGERETKAFMFTDIVTSTDLVGLIGD
                     DAWQRLLDWHDRELRAVISAHAGDEIRHTGDGFFVVFESARQALDAAVDIQRRLADHR
                     LQHGFSPTIRIGIHYTEATRQRGDYVGQGVHVAARIGDIGAGDEIVVSDALSRAVGTV
                     PYQMSQVRQADLKGVSDPVTVMSVDWHR"
     gene            7060..>7210
                     /locus_tag="VFZ80_06480"
     CDS             7060..>7210
                     /locus_tag="VFZ80_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6287113.1"
                     /translation="MTTDPVALTSQASLRDAAAAMRDRNIGDVVVTKPDGMVCGIVTD
                     RDIVVR"
BASE COUNT         1211 a         2288 c         2347 g         1364 t
ORIGIN      
        1 cgaaggaggt gagcgagtga cgattccagg ttcggcgagg aggcgagcgc cgccaaggtc
       61 gccttttcga actccgaacc gagcaccgag gccatctgga tcagatagcg ctgctcctcg
      121 ccgagacggt cgaggcgggc cgtgagcagc ccgcggagac tcgatggcac ctggctctcg
      181 tcgaactctt cgctgccgat ctcactcaag gctcggacca tctgctcgat gtagagcggg
      241 ttgccctgtg atcgctcgag gattccctct ttgatccggc tgtcgacggg agatccctcg
      301 atcagggcgt cgaacaggtt cgagctttcc tctatgtcga acggctccag ggagagacct
      361 atatagcgat gcggatgggt gcgctctgct acctcatgga gtcgccagct cggctcgtcg
      421 cgataaggcc gcatcaccaa caccagaccg agcggcgtgg tctcggcgag acccatcatc
      481 tcctctatga cggcgagcga gagatcgtcc gaccaatgca ggtcctcgag cacgacaatg
      541 gtgggtcgtc gattgaccaa ggcttgcagg taggagatca ctgcagcttg ggccctgctg
      601 ttgagaagct gggtggcgag attgtcgatg aacccttcgg ctcgctcggg aagtggaaca
      661 tcggcgacga acccgaggta tgccgcgacg tcagggtcgt cgatcccctc cgcaaccact
      721 gcttctgtca ccctgtcgaa ggccggttca gagccgtccg agaaccacca cctctggaga
      781 aggtcggcga ccgtcgagta ggggcgactc gagtcgtatg agcggctcgc cgctcgaagc
      841 cagttcactt gaccggcctg gtcataggaa taagcccgat cgactgcttc ttctgtgcgt
      901 cggccgaact cctctacgag ccgcgtcttg cctacgccgg cgtcggcgat gacggagacg
      961 atcttgcccg atccacttgt cactaccgag cgcacttcgt cgaggacggc gagctcgtcg
     1021 gcacgtccga cgatgggccc ctcacccgaa gccgaggggc gccccaagta tcgaatcacc
     1081 cggtatgccg gcacgggctc ggacttcccc ttgacatcga tggggccgag ctcctcggcc
     1141 tcgaaaacgc cgttggtcag agacagggtg tgtcccgtga cccggaccgt gtccggttca
     1201 gcggtttgct ccatccgggc cgccacgttg atggcatctc ccagagctgt gtattcgacc
     1261 cgaaggtcag accccattgc acccacgact accagaccgg tgtggatgcc cacccggacg
     1321 tgggctggca cgccccagcg cttttcgacg gtctcgctgt agccggcggt ggcagcgacg
     1381 atctcgagac cggcgcgtag tgcgcgcacc ggatcgtcct cgtgggctat aggtgcgcca
     1441 aaaaaggcga gcacggcatc accgcgaagc tgagccagcg tcccctcgta gcgatagata
     1501 ggggcgatca gcctctcgaa gaggccattc atgatgtcgg cccaatcctc ggggtcgagc
     1561 tgctccgccg acgaggtgga gccttgaacg tcagcaaaca gcatcgtgac ggttcggcgc
     1621 tcaccctcca tggcgtggcc gtcacgggcc gagcgcatct tggccaggag ctcctcgggg
     1681 agatacctgc tgagatcctc ctcggcgcgt gcaaccggcg tgggctcaga cgacgaagcg
     1741 tcttcaccga gggggttgcc gcagttgcgg cagaatcggt cgtccgggtc ggcgatcaca
     1801 ccgcacacgg ggcatgggta gggcaatgcg ttgccgcaat gggagcagaa tcttgctccc
     1861 cccgagttct catgcccgca attgcggcac ttcacactct cgtccttccg gccgtgagaa
     1921 tcgctgccga gcgacgccac ccgatcgccc tggtttcagg aggatagaga accctctgac
     1981 ccgtcgcctc cgacacggtc cggctggtag aaccgtcgcc gtgacggaaa agggaaacgc
     2041 gcgagaccgt ctgctctcgt ctgtcggcat tcgccctgat gaggtgaccc tcgccggttg
     2101 gatggcgatt ctgttcatgg tgacacaggc cagtcacgcg gtgggcgcca atgcggctga
     2161 cacgatgttc tttctccgct acggcgtcga ggaccttccc ttgatgatcc tcctgtccgg
     2221 accggcggtc atggtggcca tcgtcggtca ttcggcgggt ctcgcctgga ggggtgcgga
     2281 cacctggctg atgatcgtca tggcgttgtc ggcggcgtgg gtcggtctgc tccgggccgg
     2341 catcctcacc ggttcggccg gcatctaccc ggtgatctgg atctcgaccc aggtgctcat
     2401 cttcttgacg ctcacggtca tgtggaacgc cgcgggctcg gcgtgcacga cgcggcaggc
     2461 aaaacgcctg ttccccgttt tcgccaccgc cgccgtggcg ggcggagtca ttggaaacct
     2521 cctcgtaggt ccgctgacga cgctgttcgg cgccgagaac ctgttattgg tccagggtct
     2581 tctcttggtc atcgccacct tcttggtggt tcgagtccgc tcgttcttca cgtccggatc
     2641 aggcgagagg gaaccggtga gggtggagat gacccgggcc ttctcctcga tccggaacag
     2701 tcgtcttctc gggttggccg ccggcgtcgt gtttctcctg ttcgccctct tctacctggt
     2761 ggtgttcccc ttcagcgaga tcgtgactgc caccttcccg gccgaaacgg agatggccgg
     2821 gtttctcggg ctgttctcat cggtggccac ggctgcgacc ttcctcttct cattgctggc
     2881 gacaggtcgc ctcttctcgc ggctggggct gatagtgacc ctgatcaccg tgccggtcgt
     2941 ctatctcctg ggcttctcga cgtggctagt cgcgttcggg atcctcaccg cggtcttgtt
     3001 ccgtggcctg caatgggtgg cggtgaactc ggttcaaacg accgcctaca gcgcactctt
     3061 caacgttctc agcacgcgaa gacgggggcc ggtgctggca ctgatgaccg ccgttcccgc
     3121 tcagctgggc accatggcgg ctggtgccat cctcctactg ggcgcagatg gtctctccca
     3181 ggccggtctg ttctgggtcg gtgccctctt ggcggcggcc gcagttggcc tggttgcggc
     3241 gatgcgaccg gcctatctgg cggcagtggt cgaagcggtc cgccggggac tggtcacagt
     3301 ctgggactct cctcacaccg gtgtagtcgt cacacccgtc gaccgcgacg tggttcgcat
     3361 cctggaatca cacctgtcag atcatcgacc cagagccaga gccatagcgg ctgcgggtct
     3421 tggctcgctg ggcgacgatt cggtgctcgc tggtgtcgag gtgttgctcg acgacgacga
     3481 tcccctggtc aggtcggccg cgttcaccgc ggtctgccag atcgagccag cggccctgga
     3541 gcctcgtctc gaagctgcca ttgcagacga atcacccgag gtgaggctca acgccgtccg
     3601 ctatctggac tcgattggtg agagagatct tcccgtggcc gcgctcgcca ccgtgctcga
     3661 cgatcccgac ccgcgagttc gcgccgccgg ggcttggttg gcgcccgaag aggcaggctc
     3721 gccggtggca gtcgacatgg tgacctctgg cagacccgat gctgtccggg cggtcttcga
     3781 ggagatgctg cggcatccag gacagacgct cgggttgcgc cctcgagact tcgtggccga
     3841 tgacgatccg gcggtgcgag ctgctgcggc ggcggcgatc gcggcatcgg gctcggatct
     3901 cgaagccgtc ctgcccagcc tcgatgacag gtcggtgcgc gtgcgccgag cagctgctcg
     3961 ggctcttgcc agctcccatg agggaagaga gctcctgctc gatgtgctcg aaggcggcag
     4021 tgtcatcgcc accgaggctg cgctcgatgc catgaccccg gttgaggacc tggaccagcg
     4081 cttcactgcg tgggcagctt ccgaagcgca gcgggccagc tacctggccg gtcatcgacg
     4141 cctcctcgag acgcggctcg actctccgca gggccgctac ctggtacgcg tgctggacag
     4201 ccgggtgaat cgtctcgtcc attgggtgct gatggcgatg accaccgatg cgacgaaaga
     4261 gatcatgccg ctcgtggcac gcggggtcga agccgacgac cccgagacga acgcccaggc
     4321 gatcgaggcg ctcgagtcgg tggccgaccg ttcggtcctc tccgttctcc tgcctctgct
     4381 agagcaatcc ggcgaaggag ccggatccga tgcccacgag gacaccctcc gcgagctgtc
     4441 cggagatttc gacccttggc tgagccgtct cgccacccta tgcctcgatc gatacgcatc
     4501 cgggaatgat gacctggcaa gcttgggaga gatggccggc caagaactgg acacactcga
     4561 cgacatgggg cgagtcctcg tgttgcagca ggtgcccatg ttctccggtc ttgaccccga
     4621 ggacctgatc ctggtggccc ggttcaccaa ggaagcccgc ttcgacccca acgacccgat
     4681 ctatcgagag ggcgacccgg gaactgagct gctggtcata gtcgagggct cggcagtcgt
     4741 gagtcgaacc agggctgaag cccggcacct gatcgacacc taccacgccg gtgagcatgt
     4801 cggtgagctg tcgctgctga ccggtggtcg ccgctccgca gacgtcgagg caggatccga
     4861 cggtcttcac ggcctggtct tgggcaagtc ggagttgatg gcgatccttg aggagcggcc
     4921 cgccgtcgcc ctggggatgc tggggaccct ggcaagtcga ctcatcgagc agacctgatc
     4981 tttcacgtca ctacgctcaa caccgaggag gtgtggtgtg actctcgaag tcgcccgcga
     5041 cgcaatccgg agtaaagact ggtcgtcggc gatccaggaa ttcgaggatg ccgacgagga
     5101 gctttccccc gatgacctcg tcttgatggc cgacgctctc tggtggtcgg cccgtcccga
     5161 tgaagccgtg gacgcgtatc agagggcgtt cgaggtcttc gtgcgctcgg atcggaaggg
     5221 cagggcggct gccgttgccg ccaccctctc ctacctggcc atgcgtcgca tgtccgaatc
     5281 ggtggccatg ggctggttct cccgggcgca gcgcttgctc gaaggcgagc cggagggtcc
     5341 cggtcatgcc tggatatctc ttctcgaggc cgctcagtcc ctatttgtga tgagcgacct
     5401 cgacgcggtg aaagaggctg cggatcgcac catcgacatc gccaggagac agggagtggc
     5461 cggcgtcgag gcactggccc tcagcttcaa aggggtggct accaccaacc agggcgattg
     5521 gcgcgaggga atgaggatga tcgacgaggc aacggtgatc gcgaccgccc aagacgggga
     5581 tctccggtcg tcgagcgacg tctactgcat catgatgggg gcatgttcga gtctcgccga
     5641 ttacaagcgc gcctcggaat ggaccgagca ggccgagcgc tggatggctg tcaactctct
     5701 cggaggcttc accggggagt gtcagatcca tcgtgccgag ttcaagaggc tgcacggaga
     5761 ttggtttcag gcagagcacg acgctcggat ggcgtgcacg gagctcgagc gcttccatct
     5821 cctcaacagc cttgggtacg ccaattacga gatcggcgaa gtgcggcgtc gcatgggcga
     5881 cctcgatgca gccgaggaag ccttcacgcg ggcctacgaa tacggccatc ccgcacagcc
     5941 gggattctca cttctcatga tggatcgagg ggacctggac cgagccgcat cctccatcga
     6001 agctgccctc gagacgacgc gggccgacgg caatcgcggc gatctcctca ccaggggcca
     6061 tctgcttcct gcccagatag agatcgcact ggctcgtgac gatctcgaca ctgcgcagag
     6121 ggcggtaact cagctggaac aggtggcgga gacctacgac tccccggcct ggcaggcgat
     6181 gttgctcacc tgccgcggtg cattatcgct tgcagaaggt aggtcggccg aagcggtgac
     6241 cgcgctcgac agggcctggc gactctggca gaacgtcgag cttccctacg agactgcccg
     6301 gtcgcgagag ctcctcggcc gcgcccggat ggccgtcgga gacgagaccg cagccggtct
     6361 cgagctgagg gcggcctggt ctacgtacga gaagttggga gcgcgtctcg acctggagcg
     6421 tctccaggag tcgatgggtc cggcgggcgc ggggacgtcg agctcgggcg agcgagagac
     6481 caaggcgttc atgttcaccg acatcgtcac gtccaccgac ctcgtcgggc tgatcggaga
     6541 cgatgcgtgg cagcgtctgc tcgactggca cgatcgtgag ctccgcgccg tcatctcggc
     6601 acacgccggt gacgagatcc gtcatacagg cgacgggttc ttcgtcgtct tcgagtcggc
     6661 ccggcaagcc ttggatgctg cggtcgacat ccaacgccgc ctcgctgacc atcgtctgca
     6721 acatggcttc tcaccgacga tacgaatcgg catccattac acggaggcca cccgccaacg
     6781 tggcgactat gtgggccaag gtgtccatgt cgcagcccgg atcggcgaca tcggggccgg
     6841 cgatgagatc gtcgtttccg acgcattgtc ccgtgctgtg ggaaccgtcc cctatcagat
     6901 gagccaggtg cgtcaggccg acctcaaagg tgtgagcgat cccgtgacgg tgatgagcgt
     6961 cgactggcac cggtagacct gagttttcgc acgggggcag gcgggtaatc cccttcctcc
     7021 aaaggaggag attccatgcc acaacgcata tccgaactga tgaccactga tcccgtcgcc
     7081 ctcaccagtc aggcctccct tcgagacgcg gccgcagcta tgcgcgaccg caacatcggt
     7141 gacgtcgttg tcaccaagcc ggatggaatg gtttgcggca tcgtcaccga tcgcgacatc
     7201 gtggtgcgtg
//
LOCUS       DASUTA010000325         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1649954, whole genome shotgun sequence.
ACCESSION   DASUTA010000325 DASUTA010000000
VERSION     DASUTA010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649954"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VFZ80_06485"
     CDS             complement(<1..603)
                     /locus_tag="VFZ80_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012015575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6287114.1"
                     /translation="MTTHFFGDTAVLLGRSLRHITRSLDTIITTAIMPTAFLLLFVYV
                     FGGAIDTGSESYVNYLLPGILLITVASGVAYTAYRLFMDKKTGIFERFQSMPIARSSV
                     LWAHVLTSLVANLTSIAVVVFVALVIGFRSGAGVLGWLAVVGILILFTLALTWLAVIA
                     GLGAKSVDGASAFSYPLILLPFISSAFVPTDTMPGPVRAFA"
     gene            complement(613..1425)
                     /locus_tag="VFZ80_06490"
     CDS             complement(613..1425)
                     /locus_tag="VFZ80_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6287115.1"
                     /translation="MTTTAQSPAIRVQGLEKSFEELEVLRGADFDVKRGSIFALLGSN
                     GAGKTTIVRILSTLLEADAGSAVVNGFDVATQPAEVRGSISLTGQFAAVDEILTGREN
                     LVLIARLRRVENPAGTADDLLRRFGLSDAADRKVSTYSGGMRRRLDIAMSLIGDPPII
                     FLDEPTTGLDPEARIEVWEAIRELAGQGTTVLLTTQYLDEAERLADRIAILHDGRIIV
                     DGTVAELKQLIPPAKVEYVEKQPTLEDVFLALVGGGGTNRDAGHDHDPHASR"
     gene            complement(1422..1805)
                     /locus_tag="VFZ80_06495"
     CDS             complement(1422..1805)
                     /locus_tag="VFZ80_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1048 domain-containing protein"
                     /protein_id="HEX6287116.1"
                     /translation="MAAGWIEKITGSMEEKRRYRRYKARTAQLPANYRTAIEAVERYL
                     MYAGSITKGDIIVSMLEDLGDLFEQSAADGTPIREVVGEDPVEFAETFLRNYSDGQWI
                     NKERKRLTEAIDSVADETGTEEELE"
     gene            complement(1808..2137)
                     /locus_tag="VFZ80_06500"
     CDS             complement(1808..2137)
                     /locus_tag="VFZ80_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX6287117.1"
                     /translation="MGKQMTEMLKGTLEGIVLAILSGRSAYGYEITSWLRDQGFSDIA
                     EGTVYALLVRIEQRGLVDVEKVPSEKGPPRKVYSLNAQGRDYLEEFWRNWSFLAERLE
                     QLHEGGN"
BASE COUNT          400 a          727 c          745 g          424 t
ORIGIN      
        1 ggcgaacgcg cgcaccgggc cgggcatggt gtcggtgggc acgaacgccg agctgatgaa
       61 cggcaggagg atcagcgggt aggagaaggc gctcgcaccg tccaccgact tggctccgag
      121 tccggcgatc acggccaacc aggtcaaggc gagggtgaac aggatcagga tccccaccac
      181 cgccagccac cccagcactc ccgcgcccga tcggaacccg atcacgaggg cgacgaacac
      241 cacgaccgcg atagaggtca ggttcgccac cagcgacgtc agcacatgcg cccaaagcac
      301 cgacgaccgg gcgatcggca tggactggaa ccgctcgaag atcccggtct tcttgtccat
      361 gaacaggcgg taggcggtgt aggcgactcc cgaggcgacc gttatgagca ggatgccggg
      421 gagcaggtag ttcacatagg actcggaccc ggtgtcgatc gccccgccga agacgtagac
      481 gaacagcaac aggaaggcgg tcggcatgat cgcggtcgtg atgatcgtgt ccaggctgcg
      541 ggtgatgtgc cgcagggacc gccccaacag gacggcggtg tctccgaaga aatgcgtggt
      601 cacggtcgct ccttatctgc tcgcgtgggg atcgtggtca tgcccggcgt cgcgattggt
      661 tccgcctccc ccgacgaggg cgaggaacac gtcctcgagg gtcggctgct tctcgacgta
      721 ctcgaccttg gcgggcggga tgagctgctt gagctcggcg accgtgccgt cgacgatgat
      781 gcgcccgtcg tgaaggatcg cgatgcggtc ggcgagccgc tctgcctcgt cgagatactg
      841 ggtggtgagc aggacggtcg tgccctgccc ggcgagttcc ctgatggcct cccacacctc
      901 gatacgcgcc tcggggtcca gaccggtggt cggctcgtcg aggaagatga tcggcgggtc
      961 gccgatgagg ctcatcgcga tgtcgagacg gcgacgcata ccgcccgagt acgtagagac
     1021 cttgcggtcg gcggcgtcgc tcaggccgaa acgccggaga aggtcgtcgg cggtcccggc
     1081 cgggttctcg acgcgccgta ggcgggcgat gaggacgagg ttctcacgcc cggtgaggat
     1141 ttcgtcgaca gcggcgaact gtccggtgag gctgatcgat cctcgcacct ccgccggctg
     1201 ggtggcgacg tcgaaaccat tgacgaccgc cgatcccgcg tccgcctcca gcagcgtgga
     1261 gaggatcctg acgatcgtgg tcttgccagc tccgttggag ccgagcaggg cgaagatgct
     1321 gccccgtttc acgtcgaaat cggctccgcg cagcacttcg agttcctcga aggatttctc
     1381 caggccctgg acacggatcg ccggactctg tgctgtggtc gtcattcgag ttcctcctct
     1441 gtgccggtct cgtcggcgac actgtcgatg gcctcggtca gtcgctttcg ttccttgttg
     1501 atccactgac catccgagta attgcggagg aacgtctccg cgaattcgac ggggtcctcc
     1561 ccgacgactt cgcgtatcgg ggtgccgtcg gcggcactct gttcgaacag gtcaccgagg
     1621 tcctccagca tcgacacgat gatgtcgccc ttcgtgatcg atccggcgta catcagatac
     1681 cgctccaccg cctcgatcgc cgtgcggtaa ttcgcgggaa gctgtgccgt gcgtgccttg
     1741 taccggcggt aacgcctctt ctcctccatc gatcccgtga tcttctcgat ccatcctgcg
     1801 gccatggcta gttgcctcct tcatggagtt gttccagtcg ctccgcgagg aagctccagt
     1861 tcctccagaa ctcttcgagg tagtcccgtc cctgagcatt gagcgagtac accttgcgag
     1921 gcggtccctt ctcggagggc accttctcca cgtcgaccag accgcgttgt tcgatcctga
     1981 cgagcagcgc gtagacggtg ccctcggcga tgtcggagaa tccctgatcc ctcagccacg
     2041 aggtgatctc atagccgtag gcggaccggc ccgagaggat ggcgaggacg atcccctcca
     2101 gcgtgccctt gagcatctcc gtcatctgct tgcccacgcg gacccttctt actactcagc
     2161 gttactgact actggtacat agtaacactg aataccgcac ctgcaatggc agggcgaccg
     2221 tggtgtagga gtagagacgg aagcggagag gcgttgcccg aagggtccta ttgctctagc
     2281 cgggagcagg gtctcg
//
LOCUS       DASUTA010000326         4477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_483198, whole genome shotgun sequence.
ACCESSION   DASUTA010000326 DASUTA010000000
VERSION     DASUTA010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483198"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..640)
                     /locus_tag="VFZ80_06505"
     CDS             complement(44..640)
                     /locus_tag="VFZ80_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX6287118.1"
                     /translation="MSTAAVVLAAGGSKRLGRTKQLEPWGETTLLSQVVARTREFPVD
                     EVWVVLGHDAERILEETDLGDAYVVENLEWEEGIASSLRVVLDALTRLSRAEQVLIVI
                     GDQPDVPVETVETLMDSHAREGRPVTLPKYRYNWGNPVLVDRSLWPRLMSLEGDEGAK
                     RLWQAHPEWVNEVWFADTGPRDVDTEADVAELRPRHSA"
     gene            complement(702..1817)
                     /locus_tag="VFZ80_06510"
     CDS             complement(702..1817)
                     /locus_tag="VFZ80_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287119.1"
                     /translation="MEGDRIAGLRSRDGSLISVYAGRPSPGGFGALLSDLVKPLRERS
                     EDLDRRVRMSVRSDTERIHDLADQLEIDSAPGYAIFASDIDGTFLIQPLSHPAPNVST
                     IGPRPYMRPLRAMPRALRSGIIVADNTVARTFVAMEGIVEEIHAPIGADVGKRSFGGF
                     SGYDEHNVRARADEVTAKLWREAGERLLDRHVERPFDYLAIGSHDEKVDEISRNLHPY
                     LTRLKRETFTASPQTISVPGLRAEVLEMDKRVRRHRQAALAGRVCDTAWSGGNAVIGL
                     NEVLDAANAQAIDTLVVAGPFSRPGAICNECGYLARGGVECPVCGSALFAVDDVVGAV
                     MDATVSAGGTVSQIRVASPLDREGVGALTRFPVPVAR"
     gene            complement(1897..2352)
                     /locus_tag="VFZ80_06515"
     CDS             complement(1897..2352)
                     /locus_tag="VFZ80_06515"
                     /inference="COORDINATES: protein motif:HMM:NF020989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HEX6287120.1"
                     /translation="MSHFTTIDGKIEVRLSSEERLFLGDVLPLLAGIGARGSDPAADR
                     LQVPVYLDDPEANEEWWRLMGSDLDAGRRSDRAVFERTISTEGTVALSNAEADAVLRV
                     LNEARLALGARFGIDVEEDHDELPEESRQVLDYLGWIQEELTLELMRAL"
     gene            complement(2349..2633)
                     /gene="clpS"
                     /locus_tag="VFZ80_06520"
     CDS             complement(2349..2633)
                     /gene="clpS"
                     /locus_tag="VFZ80_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX6287121.1"
                     /translation="MLAQTTPVIVPDTDEGIDLDEPWQVVVWNDPVNTMTYVVYVFRK
                     LFGYDEPKATELMLQVHHEGRAIVSDGPREQAELDCFRLQNHGLWATIEQ"
     gene            complement(2670..3737)
                     /locus_tag="VFZ80_06525"
     CDS             complement(2670..3737)
                     /locus_tag="VFZ80_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6287122.1"
                     /translation="MARDRSGVTPDGPVRIFFSNPIAVIGFAFILLFASMVVIHPLLM
                     NTLWAGEDDVYDPVTGYDAPILEVEVVRTVTDPEIQVDVATARIADITASVGDVIEIR
                     VQPAPPSGAHWFGTDVFGRDVFSMILAGAWPTFVVGLSAALVSAAVAVTFSISSATFK
                     GRVDRTLSRVSDVLLLLPAPLAMIILAGGTTGEFLTPLNFGVTYGLLAGASTAAIVLR
                     SHALATVERPFMDAARVSGASGWYLARRHLLPHMIPLAAVTMVSSVVGAVVAHGFASW
                     LAYSDDLTNWGAIMFVAIGFSELQGVFAWNVLIAGAGAISMFCAGFYMVSLGLKDVAY
                     RGSEEQRSTRPGWARRPEVRV"
     gene            complement(3727..>4477)
                     /locus_tag="VFZ80_06530"
     CDS             complement(3727..>4477)
                     /locus_tag="VFZ80_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6287123.1"
                     /translation="LRNSGWSALGRLAELDVETWASSQLTPNQLLWRIFFVFVASALV
                     LWLIESLTWRLTRRRIPRLVFVVAMVAVPLLIWSQMGITGQVLDVVSAMSLLIVAVVI
                     LTFGEVLLVTKASMDDVMMEDYVMVARAKGLPERQVRDHHAARTALLPVLSRFTVAIP
                     YFLTGLVILEVAFGGGANLNIGGVTMRFQPPVGLGSLLFESLSNQDTPLLIGALLIVG
                     VLTLMIRIVLDVVHAALDPRIRFAGGESNGT"
BASE COUNT          906 a         1467 c         1387 g          717 t
ORIGIN      
        1 gaacgatcaa agaacgaata tagacaacga gactcatggc gaatcaggca gagtgccggg
       61 gcctcaactc ggccacgtcc gcctccgtgt ccacgtcgcg agggccggtg tctgcgaacc
      121 agacctcatt cacccactcc ggatgtgcct gccacaaacg cttggctcct tcgtctcctt
      181 cgagcgacat gagccgcggc caaagtgaac ggtcgacgag aaccgggttc ccccagttgt
      241 acctgtactt ggggagagtc accggccgac cctctctggc gtgagagtcc atgagggtct
      301 ccacggtctc caccgggaca tccggctgat cgccgatcac gatgagcacc tgctcggccc
      361 gggagagcct ggtcaaggcg tcgaggacga cgcgaagaga agatgcgatg ccttcctccc
      421 actccaggtt ctccaccacg taagcgtcgc ccagatcggt ctcctcgaga atccgctcgg
      481 catcgtgacc gaggaccacc cagacctcgt cgacggggaa ctccctggtt ctggcgacga
      541 cctggctgag cagggtggtc tcgccccacg gctcgagctg cttggtgcga ccgagacgct
      601 tcgagccgcc cgcggccagg acgacggcgg cagtggacat ggctcgggaa tctagccaac
      661 gaccctctcc taccgagaga tggccgggcc gcacaccgac attatcgggc gacggggacc
      721 gggaaacggg tgagagcgcc gacgccctcc cggtcgagag ggctggccac tctgatctgc
      781 gaaacggtcc ctccggcgga gactgtggcg tccatgaccg ccccgacgac atcatcgacc
      841 gcgaacaggg ccgacccgca gaccgggcac tccaccccgc cacgagccag gtagccgcat
      901 tcgttgcaga tcgcaccggg ccgactgaag gggccggcca ccaccaaagt atcgatcgcc
      961 tgtgcgttgg ccgcatcgag aacctcgttg agcccgatga cggcgttacc cccactccat
     1021 gccgtgtcgc aaaccctccc cgccagagca gcctggcggt gacggcgaac acgcttgtcc
     1081 atctccagga cctcggcgcg cagacctggg accgagatgg tctgcgggct cgcagtgaac
     1141 gtctcgcgct tgagccgggt cagataggga tggaggttgc gcgatatctc gtcgaccttc
     1201 tcgtcgtggc tgcctatcgc caggtagtcg aatggccgct cgacatgcct gtcgaggagg
     1261 cgctcccccg cctccctcca cagcttggcc gtcacctcgt cggcacgggc tctcacgttg
     1321 tgctcgtcat agccggagaa cccaccaaag ctacgtttgc cgacgtcagc gccaatcggg
     1381 gcgtgaatct cttcgactat cccttccatg gcgacgaacg tgcgggccac cgtgttgtcg
     1441 gcaacgatga tgccgctgcg gagagcgcgg ggcatcgccc gcagcggtct catgtagggg
     1501 cggggcccga tcgtggacac gttgggagcg ggatggctca gcggttggat caagaaggtc
     1561 ccgtcgatgt cggaggcgaa tatggcgtaa ccgggtgcgg agtcgatctc gagctggtct
     1621 gccaggtcgt ggattcgctc ggtgtcggac cgaacactca tccgcacccg ccgatccagg
     1681 tcctcggaac gctctctcaa gggcttgacc agatcggaga ggagagctcc gaaacccccg
     1741 ggactgggtc ggccggcata cacggagatg agggagccgt cacggctccg gaggccggct
     1801 atccggtcgc cttccactga acctcccttt cgacactgca gaagtgccaa gctctcggac
     1861 tcagtcaccg aacctacaca ctcgagcgag aacgcgtcac agagctcgca tcagctccaa
     1921 tgtgagctcc tcttggatcc agcccagata gtcgagaacc tggcgactct cctcggggag
     1981 ctcgtcatgg tcctcctcga cgtcgatccc gaacctggcg cccagagcaa gtcgcgcctc
     2041 gttcaacacg cgtaggaccg catcagcctc ggcattggag agggcgacgg tgccttcggt
     2101 ggagatcgtc ctctcgaaga cggcacggtc ggaccgacgc ccggcgtcga ggtcggagcc
     2161 catgagcctc caccactcct cgttggcctc gggatcgtcg agatagacag gcacctggag
     2221 acggtcggcg gcgggatcgg acccacgggc cccgataccg gcgagaagcg ggagcacatc
     2281 gccgaggaag agacgctctt ccgagctcag cctcacctcg atcttcccgt cgatcgtcgt
     2341 gaagtgactc attgctcgat cgttgcccaa agtccgtgat tttggaggcg aaagcagtcg
     2401 agctctgcct gctcccgcgg gccgtcggag acgatcgctc ttccctcgtg atgaacctga
     2461 agcatcaact ccgttgcctt cggttcgtcg tagccaaaca gcttccggaa gacatagacg
     2521 acataggtca tcgtgttcac cgggtcattc cagaccacga cctgccatgg ttcgtcgagg
     2581 tcgatgccct cgtcggtgtc tgggacgatt actggagtgg tttgagcaag catgtgaaga
     2641 aggctccgcg gccaacctta ggtgggtgct cagactctca cctctgggcg gcgagcccag
     2701 cccggccgtg tcgagcgctg ctcctcgctg cctctgtatg cgacgtcctt caaccccagc
     2761 gagaccatgt agaacccggc gcagaacatg gaaatcgccc cggccccggc gatgaggacg
     2821 ttccatgcga acaccccctg caactcggag aagccgatgg cgacgaacat gatggcaccc
     2881 cagttggtga ggtcgtcgct gtaggcgagc cacgaggcga agccatgggc caccacggcc
     2941 ccgaccaccg atgacaccat ggtgaccgcc gccaggggga tcatgtgtgg cagcaggtga
     3001 cggcgggcga gataccagcc ggaggccccc gagactcggg cagcatccat gaaaggacgc
     3061 tcaacggtgg cgagggcatg ggaccgaagg acgatcgcgg cagtgctggc tccggccagg
     3121 agcccgtagg tgactccgaa gttgagcggt gtcaggaact caccggtcgt gccaccggcg
     3181 aggatgatca tcgccagcgg ggcggggagg aggagcaaca catccgagac cctggagaga
     3241 gtccgatcca cccttccctt gaaggtcgca gaggagattg agaaggtcac ggcgacagct
     3301 gcggaaacca atgcggcact cagaccaacg acgaatgtgg gccacgcccc ggcgaggatc
     3361 atcgagaaca cgtcgcgccc gaatacatcc gtgccgaacc agtgtgcgcc gctcggcggc
     3421 gcgggctgca ccctgatctc gatgacatcg cccacactgg cggttatgtc cgcgatccga
     3481 gcagtggcca catccacctg aatctcagga tcggtgacgg tccggaccac ctcgacctcg
     3541 aggatcgggg cgtcgtaacc ggtcaccggg tcgtacacat cgtcctcacc ggcccagagg
     3601 gtgttcatca gcaacgggtg gatcacgacc atcgaggcga acagcagaat gaacgcgaac
     3661 ccgatgaccg cgatcgggtt gctgaagaag atcctcaccg gcccatcagg ggtgacacca
     3721 cttcggtcac gtgccattcg attcaccacc tgcaaaccga atacgtgggt cgagggcggc
     3781 atgcaccaca tcgagcacga tgcgaatcat gagggtcagc acgccaacga tgagcagggc
     3841 accgatcagc agcggggtgt cctgattcga gagagactcg aagagcagtg acccgagccc
     3901 caccggtggc tggaagcgca tcgtgacccc gccgatgttc aggtttgccc cgccgccgaa
     3961 cgccacctcg aggatcacca gcccggtcag gaagtagggg atcgccacgg tgaagcggct
     4021 gagcactggc aacagggctg tgcgcgccgc gtgatgatct cgcacctggc gctccggcaa
     4081 ccccttggcc cgggccacca tcacgtagtc ctccatcatc acgtcatcca tcgatgcctt
     4141 ggtgacgagg agaacctcgc caaacgtcag aatcacgacg gcgacgatca gcagcgacat
     4201 cgccgagacc acgtcgagca cctgacccgt gatgcccatc tggctccata tcagaagagg
     4261 gaccgcgacc atggcgacca cgaagaccaa ccggggaatc ctccgtctcg tcaatctcca
     4321 cgtcaggctc tcgatgagcc aaaggaccaa tgcgctggcg acaaacacga aaaagatccg
     4381 ccacaggagc tgattcggcg tcaactgtga actcgcccag gtctccacgt cgagctcggc
     4441 cagacggccc aacgcagacc aacccgagtt gcggagg
//
LOCUS       DASUTA010000327         2339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_97403, whole genome shotgun sequence.
ACCESSION   DASUTA010000327 DASUTA010000000
VERSION     DASUTA010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97403"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VFZ80_06535"
     CDS             complement(<1..350)
                     /locus_tag="VFZ80_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX6287124.1"
                     /translation="MKSLLVANRGEIAVRVIRTAQDMGIRTIAIFSELDRDARHVDIA
                     DEAWNVGGAPAAESYLNQEAILRVAHESGAEAIHPGYGFLSENAGFARAVDTAGIIWV
                     GPSGDAIEAMGDKIT"
     gene            complement(347..1897)
                     /locus_tag="VFZ80_06540"
     CDS             complement(347..1897)
                     /locus_tag="VFZ80_06540"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014556696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX6287125.1"
                     /translation="MGTEARIDQLRTLRDEALHSGTQRAVARQHEAGKLTARERLDVL
                     LDQGSFNEIDQFVKHQSTGFGIEDKKPPGDAVVTGHGTIDGRPVFVFAEDFTVFGGSL
                     GKVVADKICKILDMTLETGAPVIGLKDSGGARIQEGVSSLDGYGRIFERNVRASGVIP
                     QISVIMGPCAGGAVYSPALTDFVFQVDQTSHMFITGPDVIKAVTGEEVTMEDLGGASA
                     HASRSGVTHFVYPNGQDALEAVRYLLSFLPRNNMEMAPYYAPADRSDRADDALDAIIP
                     DESNQPYDMREVIETVLDSEEFFEVHEQWAKNIIVGFGRLDGHSVGIVANQPAVLAGT
                     LDIAASEKAARFVRFCDAFNIPILTFVDVPGFLPGVDQEHGGIIRHGAKLLYAYCEAT
                     VPRISVITRKAYGGAYLVMNARGIRADLVFAWPSAEIAVMGAPGAVNIIHRRELAQSD
                     DPESRRTELIADYEKRFNNPYVAAELGLVDEIIEPRETRLKLIRSMEMLRTKRESLPP
                     KKHGNGPQ"
     gene            1841..>2339
                     /locus_tag="VFZ80_06545"
     CDS             1841..>2339
                     /locus_tag="VFZ80_06545"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287126.1"
                     /translation="MEGFVSKRSELIYARLGAQDFSLSSRSASVSYDSPGDKLATPYF
                     QLRLESVPSTQDVAREQFDRLPLLVIASSQTHGRGRSGVDWENADRALAASLIFEHDE
                     GDHRPFSLMAGVAAVRSTEGTRLKWPNDVLTEEGLKAGGILVERDRATTVIGLGMNLW
                     WPSPPE"
BASE COUNT          418 a          724 c          735 g          462 t
ORIGIN      
        1 gtgatcttgt cgcccatggc ttcgatggcg tcacccgatg gcccaaccca gatgatgccc
       61 gccgtatcca cggcgcgtgc gaaccctgcg ttctcggaga gaaacccata gccgggatgg
      121 atggcctccg cacctgactc atgcgcaact cggaggatcg cctcttggtt gaggtagctc
      181 tccgccgccg gggcgccacc gacgttccac gcctcatcgg cgatgtctac atgtcgggca
      241 tccctgtcga gctcggagaa gatggcgatg gtgcggatgc ccatgtcctg ggcggtgcgt
      301 atcaccctga ccgcgatctc cccgcgattg gcgacgagca ggctcttcac tgcggcccgt
      361 tgccgtgctt cttcggtgga aggctctcgc gcttggtgcg cagcatctcc atgctgcgta
      421 tcagtttgag gcgtgtctcc cggggttcga tgatctcgtc gacgagtccg agctcggcgg
      481 ccacgtacgg gttgttgaat cgcttctcgt agtcggcgat caactcggtt ctcctcgact
      541 cgggatcgtc ggactgcgcc agctcacgtc ggtgaatgat attcaccgca ccgggagcac
      601 ccatgaccgc gatctccgcg gatggccagg cgaacacgag atcggcccgg attccccggg
      661 cgttcatgac caggtaggca ccgccgtagg cctttctggt gatgaccgag attcggggga
      721 cagtcgcctc gcaatatgcg tagaggagct tcgccccgtg acggatgatt cccccatgct
      781 cttgatcgac tccggggaga aagccgggaa cgtcgacaaa ggtgaggatg gggatgttga
      841 aggcgtcaca gaagcgcacg aaccgtgccg ctttctccga tgcggcgatg tccagagtcc
      901 cggccagcac cgccggctgg ttggcaacta tcccaacgga gtggccgtcg aggcggccaa
      961 agccgacgat gatgttcttg gcccactgct cgtggacctc gaagaactcc tcggagtcga
     1021 ggacggtctc gatgacctcg cgcatgtcgt atggctggtt cgactcgtcc gggatgatgg
     1081 cgtcgagagc gtcgtcggca cgatcggagc ggtcggcggg ggcgtaatag ggagccatct
     1141 ccatgttgtt gcgaggaagg aacgacagga gatatctcac tgcctccaga gcgtcctgtc
     1201 cgttggggta gacgaaatga gtgacccccg acctggaggc atgggcagag gcgccgccga
     1261 ggtcctccat ggtcacctcc tcaccggtca ccgccttgat gacatcggga ccggtgatga
     1321 acatgtgcga ggtctgatcg acctggaaca cgaagtcggt gagagccggc gagtacaccg
     1381 cccctccggc gcagggcccc atgatcaccg agatctgtgg aatgaccccg gatgccctca
     1441 cattgcgctc gaagattcgc ccatatccgt cgagcgatga caccccctcc tggatgcggg
     1501 ccccacccga gtccttgagc ccgatcaccg gggccccggt ctccagggtc atgtcgagga
     1561 tcttgcagat cttgtcggcg acgacctttc cgagactgcc tccgaaaacg gtgaagtcct
     1621 cggcaaagac gaacaccggt ctgccatcga tagtcccgtg acccgtaacc acggcgtctc
     1681 cgggtggctt cttgtcctcg atgccgaatc cggtcgattg gtgtttgacg aactggtcga
     1741 tctcattgaa cgacccttgg tcgagaagga cgtccagtcg ctcgcgggcg gtgagtttgc
     1801 ctgcctcgtg ctggcgggcg acggctcgct gggttccgga gtggagggct tcgtctcgaa
     1861 gcgttctgag ttgatctatg cgcgcctcgg tgcccaagac ttctccctat cctcgcgatc
     1921 ggcctcagtt tcgtatgact cgccgggaga caagttggct acaccatatt tccagcttcg
     1981 cctcgagagc gtcccatcca cacaggatgt cgcccgggag caattcgaca gactcccttt
     2041 gctcgtaatc gcatcgtccc aaacccatgg ccgaggcaga tcaggggtgg attgggagaa
     2101 tgccgaccgg gcattggctg cttcactcat cttcgaacac gacgaaggcg accatcgccc
     2161 cttctccttg atggccggcg tggccgcggt ccgctccacc gagggcaccc ggctcaaatg
     2221 gcccaacgac gtgttgaccg aggagggtct gaaagcaggg gggatcctcg tcgagagaga
     2281 ccgggcaacg actgtgatag gtctcggcat gaacctctgg tggccgagcc cgcctgaag
//
LOCUS       DASUTA010000328         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1133854, whole genome shotgun sequence.
ACCESSION   DASUTA010000328 DASUTA010000000
VERSION     DASUTA010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133854"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="VFZ80_06550"
     CDS             <1..845
                     /locus_tag="VFZ80_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6287127.1"
                     /translation="SLAVASARHRWKLLSRSPGFWIGAFLVLMWTVFAIFGDFITPYD
                     PLGTPQDILNRLQPPSGEHWFGTDSLGRDVQSRVLAGARQVLTVSLAATVLGTVLGTI
                     IGLVIGYFGGIVDDAISRILDAFLSIPLVITGMIALAALGPNTLGVIIVIGLVFTPII
                     AKTVRAAVLGERQLDYVTAAKLRKENSLYILFVEILPNVFSVVIVEFTVRLGYAIFTV
                     LALSFLGFGVEPQTPDWGKDIFDHYGFLTGNIWWPVLFPALAIASLVVGVNLLADAIS
                     QVYE"
     gene            859..>2073
                     /locus_tag="VFZ80_06555"
     CDS             859..>2073
                     /locus_tag="VFZ80_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX6287128.1"
                     /translation="MSDQPVLEVKDLAIDYKIGTEWKTAVKGINFAIRPGESYGLVGE
                     SGCGKTTIAMAIMHYLSRNGRVSAGSIHLNGREFTELDRRQLRDARVNEISMVYQDPG
                     TALNPSIKIGAQVADVFELMGVPKGQHRDRVIEILRKVQIADPGRVVDRYPHQLSGGM
                     LQRVVIAMAIASRPSLLVLDEPTTGLDATVEAEVLDLVAGLRDEIGTSVLFISHSLGV
                     IRKMCDRTCVLYAGRMMAEGPTEEIFTEPRHPYTVGLLRCLPNPDAGTRDELSLDTIP
                     GFLPPLGAEIQGCVYADRCAMSEDICLEVEPGLNDLGGGRVSRCHFPEEAPNLPRTVA
                     PEPVKRAHSDQTLVEIKGASKTFMQEGHPVYALSDIDLEINVGETVGLVGESGSGKST
                     LAKVLLGIHAPDD"
BASE COUNT          356 a          676 c          636 g          405 t
ORIGIN      
        1 tgtctctcgc cgtagcctcc gcgcgccacc gatggaagct cctctctcgg tcgcctggat
       61 tttggatcgg cgccttcctc gtcctgatgt ggacggtctt cgcgatcttc ggcgacttca
      121 tcacccctta cgatcctctg ggaacgccac aggacatcct caaccgcctt cagccgccat
      181 ccggcgagca ctggttcggc accgactccc tgggtcgaga cgtccaatcc agggtcttgg
      241 ccggcgctcg acaggtattg acggtctcgt tggccgccac tgtcctcgga acggtgctcg
      301 gcacgatcat cgggctcgtc atcggctact tcggcgggat cgtcgacgat gccatcagtc
      361 gcatcctcga cgccttccta tcgataccgc tcgtcatcac agggatgatc gccctggcgg
      421 ccctggggcc gaacactctt ggggtcatca tcgtcatcgg gctggtcttc acgccgatca
      481 tcgccaagac cgtccgtgcc gcagtgctcg gcgagcggca gctcgattat gtgaccgcgg
      541 caaagcttcg caaggagaac tcgctctaca tcctcttcgt cgagatcctc cccaacgtct
      601 tcagcgtcgt gatcgtcgaa ttcacggtca ggctgggata tgccatcttc accgtgctcg
      661 ccctctcctt cctcgggttc ggcgtcgaac cgcagacgcc agactggggg aaggacatct
      721 tcgatcacta cggcttcctc accggcaaca tctggtggcc ggtccttttc cctgccctgg
      781 ccattgcgtc tctggttgtc ggggtgaatc tgttggcgga tgcgatcagc caggtctacg
      841 aatgactcga gagcacccat gagcgatcaa cccgtactcg aggtgaagga cctcgccatc
      901 gattacaaga tcggcacgga gtggaagacg gccgtgaagg gcatcaactt cgcgatccgt
      961 cccggcgagt catatggtct ggtgggggag tcgggctgcg gtaagaccac catcgccatg
     1021 gcgatcatgc actacctctc ccgcaacgga cgtgtcagcg ccgggtcgat acatctaaac
     1081 ggtcgcgagt tcaccgagtt ggacagacgg cagctgcgcg atgccagggt gaacgagatc
     1141 tcgatggtct atcaggatcc ggggaccgca ctcaacccct cgatcaagat cggcgcccag
     1201 gttgccgacg tgttcgagct catgggcgtg cccaaggggc agcatcgaga tcgggtgatc
     1261 gagatcctgc gcaaggtcca aatcgccgat cctggccgag tggtcgaccg gtacccacat
     1321 cagctgtccg gcgggatgct gcagcgggtc gtcatcgcca tggcgatcgc atcccggcct
     1381 tcgctgctgg tgctcgacga gccgacgacc ggtctcgatg ccaccgtcga ggccgaggtc
     1441 ctcgatctgg tggccggttt gcgtgacgag atcggcacct cggtgctgtt catcagtcac
     1501 agcctgggag tcatccgcaa gatgtgcgac cgcacctgtg tcctgtacgc cgggcggatg
     1561 atggcagaag gccctaccga ggagatcttc accgaaccgc gtcacccgta cacggtcggg
     1621 ctgcttcgtt gtcttcccaa ccccgacgcc gggacccgtg acgagctgag tctcgacacc
     1681 attcccgggt tcctgccccc gcttggagcc gagatccagg gttgtgtcta cgccgatcgc
     1741 tgcgccatgt ctgaagacat ctgcctcgag gtggagcccg ggctgaacga cctcggcggg
     1801 ggccgggtga gccgttgcca cttcccggag gaggctccca acctgccccg aaccgtggcc
     1861 ccggagccgg tgaaaagggc ccacagcgac cagaccctgg tcgagatcaa gggggcctcg
     1921 aagacgttca tgcaggaggg gcatccggtc tacgccttgt ccgacatcga cctggagatc
     1981 aacgtcggcg agacggttgg gctggtgggg gagtcgggct cgggcaagtc gacgctggcc
     2041 aaggtcctcc tcggcatcca tgcccccgac gac
//
LOCUS       DASUTA010000329         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1003700, whole genome shotgun sequence.
ACCESSION   DASUTA010000329 DASUTA010000000
VERSION     DASUTA010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003700"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFZ80_06560"
     CDS             <1..556
                     /locus_tag="VFZ80_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287129.1"
                     /translation="VAVDDLPSDDGGSGDGGGDDGGFPLAGAVPGSFTVDGESFDGVD
                     VYACEPFSFGSDPDPRDLSLFGYVGGTSGLNVDVSHSQGFDMSDGTQFDQMTLNVFYS
                     RQGSAGLEQFEAGAQNRADGSWFTTDPETTEQADLAEPPVAIDGNRVSGGLASLEQTW
                     PDEGAATVDVTFDFGIPTETNEEC"
     gene            complement(636..908)
                     /locus_tag="VFZ80_06565"
     CDS             complement(636..908)
                     /locus_tag="VFZ80_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287130.1"
                     /translation="MADSYEGPVRIMGGDGILLTTGQAVLENDEELGNWTGVVQTLRG
                     TAVAGKALIVELEIPEGGRGRAQLTPLRVVEDRAQSTVTGFGTPPF"
     gene            complement(908..1279)
                     /locus_tag="VFZ80_06570"
     CDS             complement(908..1279)
                     /locus_tag="VFZ80_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012797304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6287131.1"
                     /translation="MTDTTRAVFVISVAAELAGMHPQTLRIYERKGLIDPFRTPGGTR
                     RYSQEDIERLQLIQEMTAEGMNLEGVRRVLALQEENRRLKRKLDRLRDKIEEMEEAAE
                     TSGRPPEILLRSQVRTIYGER"
     gene            complement(1293..>2056)
                     /locus_tag="VFZ80_06575"
     CDS             complement(1293..>2056)
                     /locus_tag="VFZ80_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX6287132.1"
                     /translation="QRGRGTGPSRGADLTTELTIGFEDAAFGVETEVVVEGAAPCSRC
                     AGSGAEPGSSVTTCPTCGGRGVVAQNQGFFSFSQPCPQCHGSGRLIEDPCSKCRGSGT
                     ENRRRNVKLRVPAGVKDSQTLRLRGKGAPGSNGGPSGDLMVRIRVAPSRTFGRRGNDL
                     TLKVPITYTEATLGTKLEVPTLNGGVKVKIPSGTPTGKTFRVRGKGINPDRGKTGDLL
                     VTVEVAVPQKLSKDEKRLLEELAEHETDDIRSHLR"
BASE COUNT          345 a          667 c          637 g          407 t
ORIGIN      
        1 ggtcgcggta gacgatctgc cgagtgacga cggcggctcg ggtgacggtg ggggcgacga
       61 cggtggcttc cccttggcag gagccgtacc tgggtcgttc acggtggatg gagagtcgtt
      121 cgacggtgtc gatgtctatg cctgtgagcc tttctcattc ggctccgacc ccgatccgcg
      181 agatctctcg ttgttcgggt acgtcggcgg cacctccggg ctcaacgtgg acgtgagcca
      241 cagccagggc ttcgacatga gcgatgggac tcagttcgat cagatgacct tgaacgtctt
      301 ctactcacga cagggctcgg ccgggctcga gcaattcgaa gccggcgcac agaatcgtgc
      361 cgacggctcc tggttcacga cagaccccga gaccaccgaa caggccgacc tcgccgagcc
      421 tccggtagcg atcgacggca accgtgtctc tgggggtctg gccagtctgg aacagacctg
      481 gcccgacgag ggggcggcga ccgtggatgt caccttcgat ttcggtatcc cgaccgagac
      541 caacgaggag tgctgacgtt cctccgaacg cgaacccagg gttgcagaca ccgctcagcg
      601 aagtctgaaa ccctgggttt ggaaactggg ctggatcaga acggtggcgt cccgaacccg
      661 gtgaccgtcg attgcgcccg gtcctcgaca acacgcagtg gtgtcagctg ggcgcgtccc
      721 cgcccaccct cgggaatctc caactcgacg atcaaagcct tcccggcgac ggcggtgcca
      781 cgcagggtct gaacgacgcc ggtccagttt cccaactcct cgtcattctc gaggaccgcc
      841 tgaccggtgg tgagaaggat tccgtcacca cccatgatcc gaaccggccc ctcgtagctg
      901 tcggccatca tcgctccccg tagatggtcc gaacctggct gcgcagcaag atctctggag
      961 gtcggcccga cgtctcggcg gcctcctcca tctcctcgat cttgtcgcgg agacgatcga
     1021 gcttcctctt gagacgtcga ttctcttcct ggagggcgag cactcttcgc actccttcga
     1081 ggttcattcc ctccgcggtc atctcctgga tgagctggag ccgttcgatg tcctcctggg
     1141 agtaacgccg tgttccacca ggcgttcgga acgggtcgat caggcccttg cgctcataga
     1201 tgcgcagggt ctgagggtgc atcccggcca gctccgccgc caccgagatc acgaacaccg
     1261 cacgtgtggt atctgtcatc gtgtcgaccc catcatctca gatgagaccg aatgtcatcg
     1321 gtctcatgct cggccaattc ctcgagcagt cgtttctcgt ccttcgagag cttctgaggc
     1381 actgctacct cgacggtgac caggagatct ccggtcttgc cacgatcagg gttgatgccc
     1441 ttgcctctga cccggaatgt ctttccggtg ggcgtgcccg aggggatctt gaccttgacc
     1501 ccgccgttca gggtgggcac ctcgagcttg gttcccagcg tggcctcggt gtaggtgatc
     1561 ggcactttga gggttagatc gttgcccctc cggccgaatg tccggctcgg ggccacccga
     1621 atgcggacca tgaggtctcc gctgggacct ccgttggagc caggcgcgcc cttgcctcgc
     1681 aatctgagag tctgggagtc tttgacaccg gcaggaaccc gaagtttcac gttgcggcgg
     1741 cgattctcgg ttccagatcc tcgacacttg ctgcaagggt cttcgatgag ccgccccgag
     1801 ccgtgacact gcgggcatgg ctgggagaac gagaagaatc cctggttttg cgctaccact
     1861 ccacgtccgc cacatgtcgg gcaggtggtg accgaggagc ccggctcggc accagatccg
     1921 gcgcaacggg aacagggcgc cgctccctcg acgacgactt cggtttcgac accgaaggca
     1981 gcgtcctcga agccaatcgt gagctcggtc gtcagatcgg cgccacgact gggcccggtg
     2041 cctcggcccc gctgct
//
LOCUS       DASUTA010000330         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1003821, whole genome shotgun sequence.
ACCESSION   DASUTA010000330 DASUTA010000000
VERSION     DASUTA010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003821"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1721)
                     /locus_tag="VFZ80_06580"
     CDS             complement(<1..1721)
                     /locus_tag="VFZ80_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX6287133.1"
                     /translation="MANLNDVVLLLAAEGGSAAAPLSEHEVLVFLVQLALLVGVARMF
                     GWLMKSVGQPPVVGELLAGVVLGPTLFGRIAPDTFEWVFREPTVTSVVFGIAWLGVIM
                     LLVVIGFETDLGIIARFRNAALSVSAGALLIPLAATATLAFFVPESFIGAGPGGEGVD
                     RAVFAGFLALALSVSALPVVAKILQDMGYLRRNFGQVTLAAGMTMDAVGWLILAALSG
                     IALDGLQLDRLGISFGGLVIFLLLSFTVLRWVIDRLFRYVMARGSSITAALSISIVAA
                     LIGGAVTQALNIEAILGAYIVGILLAGLRHQLPQVRSILETVTAALLAPVFFAFSGLR
                     VDVGLLDTSTVILWAGVLIVVAITAKILGTVVGGFFAGVRGREALALGSGLSALGAMG
                     VVVAIVGLNIGVVSETGYTVMVLAAVVTSIVAPQLLKLVVRGWDIPHEEAQRLNEEQV
                     KEASEILRSRRILIPTRGGANSRYAAQLVSEVFPDAEMTVLVVDLAKPGMFRRMMRRV
                     EGEPGDPEDVLAELPEARTIRRLARDPAAAIAKESSLGYDLVVLGATETGEGTFSSVI
                     DRVLSMI"
     gene            complement(1725..>2056)
                     /locus_tag="VFZ80_06585"
     CDS             complement(1725..>2056)
                     /locus_tag="VFZ80_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018830673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEX6287134.1"
                     /translation="LMKTLGQDEPGLDAMVRVAYETLGLQSFLTAGEKEARAWTIPTG
                     STAPEAAGVIHTDFQRGFIAAEVIDYDKLIEAGSWLAARSGGAVRTEGKSYVMRPNDV
                     VEFRFNV"
BASE COUNT          431 a          682 c          641 g          302 t
ORIGIN      
        1 tcgatcatcg acagcacgcg gtcgatcaca ctgctgaacg tgccctcacc ggtctcggtg
       61 gctcccagga caaccaggtc gtagccgagg gagctctcct tggcgatcgc ggcggcggga
      121 tcacgtgcca gacgtcgaat cgtcctcgcc tcagggagct cggccaagac gtcctcgggg
      181 tctccaggct caccctcgac ccgacgcatc atccggcgaa acatccccgg cttggccaga
      241 tcgactacca ggactgtcat ctcggcgtca gggaacacct cactcaccag ctgggcggcg
      301 tatcggctgt tggcgccacc gcgtgtcggg atgaggatgc gtcgcgagcg aaggatctcg
      361 ctggcctcct tgacctgctc ttcgttgagt ctctgtgcct cctcgtgggg gatgtcccat
      421 cccctcacca ccagcttgag cagctgtggc gccacgatcg aggtgaccac ggcggcgaga
      481 accatcacgg tgtagcccgt ctcgctgacc accccgatgt tgaggccgac gatcgccacc
      541 actacaccca tcgctcccaa agccgagagc ccggaaccga gagccagagc ctcccggcct
      601 cgcaccccgg cgaagaagcc accgacgacc gtccccagga tcttggcggt gatcgccacc
      661 acgatcagca cgcccgccca aaggatcaca gtggacgtgt cgaggaggcc gacatcgacc
      721 cggagaccgg aaaaggcgaa gaagacaggg gcgaggagag cagcggtcac ggtctccagg
      781 atcgagcgaa cctggggaag ctgatgacgg agcccggcaa ggaggatccc cacgatgtag
      841 gcgccgagaa tcgcctcgat gttcagagcc tgggtgaccg caccaccgat cagcgccgcc
      901 acgatggaga tcgaaagagc ggcggtgatc gacgagcccc gagccatcac atatctgaag
      961 agccgatcga tgacccaacg aagcaccgtg aacgagagca gcaggaagat gacgagcccg
     1021 ccgaaggaga tgccgagccg gtcgagttgg agcccgtcca gggcgatccc tgacaacgcg
     1081 gcgaggatga gccaccccac ggcgtccatg gtcattcctg ccgccagggt cacctggccg
     1141 aagttgcggc gcagatagcc catgtcctga aggatcttgg ccaccaccgg gagagccgag
     1201 actgacagcg ccagggcgag gaaaccagcg aagaccgctc ggtcgacccc ctcaccccct
     1261 gggccggccc cgatgaatga ctcggggacg aagaaggcga gcgtggccgt ggccgccagc
     1321 gggatgagca atgcacccgc ggagaccgac agtgccgcat ttcggaacct ggcgatgatg
     1381 ccgagatcgg tctcgaaacc gatgacgacg agaagcatga tcaccccgag ccaggcgatg
     1441 ccgaacacga ccgaggtcac cgttggctca cgaaagaccc attcgaaggt gtcaggggca
     1501 atccttccga agagggtcgg cccgagaacg acaccggcga ggagctcgcc cacgaccgga
     1561 ggctgaccga cgctcttcat gagccagccg aacatgcgcg ctaccccgac cagcaacgcg
     1621 agttgcacca ggaagacgag tacttcgtgt tcgctcaacg gcgccgccgc cgagccaccc
     1681 tcggcggcga gaagaaggac cacgtcattc aagttagcca cctgtcagac gttgaaccgg
     1741 aactcgacga cgtcgttggg ccgcatgaca taggactttc cttcggttct caccgcgccg
     1801 ccggatcgcg cagcgagcca ggatccggcc tcgatcagct tgtcgtagtc gatgacctcc
     1861 gctgcgatga acccacgctg aaagtcggtg tggatgactc ctgcggcctc gggtgccgtg
     1921 gatccggtcg gaattgtcca cgccctggcc tccttctctc ctgcggtgag gaaagattgg
     1981 agcccgagtg tctcgtaggc gacccggacc atggcatcca gccccggctc gtcctgaccg
     2041 agagtcttca ttagat
//
LOCUS       DASUTA010000331         3187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1522465, whole genome shotgun sequence.
ACCESSION   DASUTA010000331 DASUTA010000000
VERSION     DASUTA010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522465"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VFZ80_06590"
     CDS             complement(<1..940)
                     /locus_tag="VFZ80_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6287135.1"
                     /translation="MITSISPTDGSILAEYQETAAAEVQTRIEVAAGLQREWEQTTFA
                     ERAIPMMKMADLLESHRDDLSVMMSDEMGKPIVQARAEVDKCAWVCRHYAEHAERILQ
                     PEHIEADRSKSYVAYRPLGVVLAVMPWNFPLWQVYRFLAPALMAGNGGLLKHASNVTG
                     SALWIDRLTVEAGFPDGLFRTLVLPSNRVDEVLEHPDVVAATLTGSDPAGRAVAKKAG
                     SLLKKTVLELGGSDPYVVLADADLEQAAATCSTSRLINGGQSCIAAKRFIVVAPVVEQ
                     FTELMAAGLEKAVMGDPRDEATTLGPMARVDLRDELH"
     gene            complement(988..1557)
                     /locus_tag="VFZ80_06595"
     CDS             complement(988..1557)
                     /locus_tag="VFZ80_06595"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX6287136.1"
                     /translation="MSRRSEESLGRVERILEEPSVWADPPGELEPALLAAISGSERIE
                     VVPTPEPSSRRWWGYAAAALVAAVIALIVFVPLGGDEPVPAAIIALSGVGVEGEAAVG
                     AADAGWWIQMKVTGLDAAPEGSFYEGWVSDGQDMISVGTFHMREGSYVTLWSGVPLRE
                     YPELLVTLQEVGAGPAPSSEVVVSGRLEG"
     gene            complement(1554..2072)
                     /locus_tag="VFZ80_06600"
     CDS             complement(1554..2072)
                     /locus_tag="VFZ80_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX6287137.1"
                     /translation="MDRAVLEQFRDGDALAVKAVYDEFGGPVYALSLSVLRDPDLAAD
                     ATQQTFLKAWRASSTYDPEREFRPWIYAIARRTAIDIYRKRKRIVPSNDIDGVTTPPG
                     LEVTWEVFEVRAALDRLPDAERVVVQLSHLEGLTHAEIADRLGIPIGTVKSRSHRAHQ
                     RLAALLSHVNEI"
     gene            complement(2284..2679)
                     /locus_tag="VFZ80_06605"
     CDS             complement(2284..2679)
                     /locus_tag="VFZ80_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX6287138.1"
                     /translation="MKARSLALVAVIALFTACSGDGDGATSTTAGSADQTTRAVDTTA
                     GGGSAAAASITIANFAFTGAESVEVGATVTVTNEDDVSHTWTSEDDVFDSGSLSQGES
                     FQYTFDEAGEYSFVCTIHPGMSGSITVEG"
     gene            complement(2742..>3187)
                     /locus_tag="VFZ80_06610"
     CDS             complement(2742..>3187)
                     /locus_tag="VFZ80_06610"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HEX6287139.1"
                     /translation="LLGVGQDATDEGQIQGTQVSVFDVTDLSDPTRLDTFTLDNGTSS
                     QVEYDHHAFLYWDGLAVVPVQQYWWEEGKDERFMGAIGLRIEDSGEINVLGDIVHPGG
                     DDTSWDWRSQILRSVVIGDHLFTVSAKGILKSDLDTLDEITWMGF"
BASE COUNT          580 a         1065 c          953 g          589 t
ORIGIN      
        1 tgtgcaactc gtcacgaaga tcgacacgtg ccatgggacc gagtgtggtg gcctcgtcgc
       61 gtgggtcccc catgaccgcc ttctccagac cggcggccat cagctcggtg aactgctcga
      121 cgaccggcgc tacgacgatg aacctcttgg ccgcgatgca cgattgacca ccattgatga
      181 gacgtgaagt cgagcaggtg gccgccgcct gttcgaggtc ggcatcggcc aagaccacat
      241 aaggatcgga gccgcccagc tcgagcacgg tcttcttcag tagggagccc gccttcttgg
      301 caaccgcccg accggccgga tcggagccgg tgagcgttgc cgccaccacg tccggatgct
      361 cgagcacctc gtcgacacgg ttcgagggga gcaccaatgt gcggaacagg ccatcaggga
      421 agcccgcctc taccgtcagc cgatcgatcc agagggccga accggtgacg ttggaggcat
      481 gcttcaacag tcctccgttg ccggccatga gcgcgggggc gaggaagcga tagacctgcc
      541 acagggggaa gttccacggc atgacggcga gaaccacgcc gagcggccga tacgccacgt
      601 acgacttgct ccgatcggct tcgatgtgct ccggctgcag gatcctctcc gcgtgctccg
      661 cgtagtgccg gcacacccag gcgcacttgt caacctcggc ccgagcctga acgatgggct
      721 tgcccatctc atcagacatc atcacggata ggtcatcacg atgagactcg agcaaatctg
      781 ccatcttcat catcggtatg gcccgctcag cgaaggtcgt ctgctcccac tcacgttgca
      841 ggcctgcagc gacctcgatc cgggtttgca cctccgccgc cgctgtttcc tggtattcgg
      901 cgagaattga gccgtcggtg gggctgattg atgtgatcat gagctgatta tgcccaactg
      961 tggccggcgc cgagagggag gagcccgtca accctcgagc cgaccagaca cgaccacctc
     1021 tgatgagggg gcaggccccg cgcccacctc ttggagcgtc accagcagct cgggatactc
     1081 ccgaaggggg acccctgacc acaaggtgac gtaactgcct tccctcatgt ggaaggtgcc
     1141 aacactgatc atgtcttggc catcgctgac ccatccctcg tagaagctgc cctcgggggc
     1201 agcgtccaga ccggtgacct tcatctggat ccaccatccc gcatcggcgg caccgacggc
     1261 ggcctccccc tccactccga ctccgctgag agcgatgatc gctgcaggca caggttcgtc
     1321 gccgcccagc gggacgaaga cgatcaaggc gatcacggcc gccaccagcg ccgcggcggc
     1381 atatccccac cacctcctgg acgagggctc aggcgtgggg accacctcga tgcgctctga
     1441 cccgctgatc gctgccagga gggcgggttc cagctctccg gggggatcag cccagacgga
     1501 tggctcttcg aggatcctct ccacccggcc gagactctct tcgctgcgtc tgctcatatc
     1561 tcgttcacat gactcagtag ggcggccagt ctctgatggg cgcgatgtga gcgcgacttg
     1621 acggtgccga tcggaatacc caaccgatct gcgatctcgg catgggtgag accctcgaga
     1681 tggctcagct gaaccacgac gcgttctgcg tccggaagcc ggtcgagggc agcgcgcacc
     1741 tcgaatacct cccaggtgac ctccagtcct ggcggcgtcg tgacaccgtc gatgtcgttc
     1801 gaagggacga tgcgctttct ctttcggtag atgtcgatgg cggtgcgtct tgctatggcg
     1861 tagatccacg gccggaactc acgttcaggg tcatatgtcg aggacgccct ccacgccttg
     1921 aggaacgtct gttgtgtggc atcagccgca aggtcgggat cgcgcaggac gctcagtgac
     1981 agcgcgtaga cgggaccgcc gaattcgtcg tagacggcct tgaccgcaag ggcgtcgcca
     2041 tctctgaact gttcgagcac cgctcggtcc acgccaccga ttctgccaga tggcacgggc
     2101 tgtggtcccc ggtggcgaat ccgcccgtcg gaccgctcga gcactcggcc gctcccgagt
     2161 ggcctgatcg tctcgactag gtcggctctc atatccggtg cctcacccac cgttacgtca
     2221 cgatccgccg gccggttcgc atcccccctg gatccccgac gctccaggcg tcggctcgtg
     2281 acatcagccc tccacggtta tggaccccga catccccggg tggatggtgc acacgaacga
     2341 gtattcaccg gcctcatcga acgtgtactg gaacgactca ccctgactca acgacccact
     2401 gtcgaagaca tcgtcttccg aagtccaggt gtgactcacg tcatcctcgt tggtgacagt
     2461 caccgtggct cccacctcca cactctcggc gcctgtgaag gcgaagttgg cgatagtgat
     2521 gctggccgct gccgccgagc ctcccccagc ggttgtgtcc acggcccggg tggtctgatc
     2581 ggccgacccg gccgttgtcg aagttgcccc gtcgccgtca ccggagcaag cggtgaagag
     2641 ggcgatgaca gcaacaaggg cgagagaacg agctttcatt gatccttcct ggacgattcc
     2701 gttgtctcta cgaaacgcga ctcggttcgg ttcgcccgcg ctcagaaccc catccaggtg
     2761 atctcgtcca gtgtgtccag gtcgctcttc aagatgcctt tcgccgacac cgtgaagagg
     2821 tgatcaccaa tgaccacaga tcggaggatc tgagaccgcc agtcccagct ggtgtcatcc
     2881 ccgccgggat ggacgatgtc acccaagacg ttgatctcgc cagaatcctc gatgcgcagc
     2941 ccaatcgctc ccatgaacct ctcgtccttc ccctcctccc accagtattg ctggaccggg
     3001 acgaccgcca ggccatccca gtagaggaag gcatggtggt cgtactcgac ctgcgagctc
     3061 gtcccattgt cgagtgtgaa ggtgtcgaga cgggtggggt cggagagatc ggtcacatcg
     3121 aacaccgaga cctgcgttcc ctgtatctga ccctcgtcgg tggcgtcttg accgactccc
     3181 aggagga
//
LOCUS       DASUTA010000332         4985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1652850, whole genome shotgun sequence.
ACCESSION   DASUTA010000332 DASUTA010000000
VERSION     DASUTA010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1652850"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VFZ80_06615"
     CDS             <1..658
                     /locus_tag="VFZ80_06615"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6287140.1"
                     /translation="RPPGEINVFARPGVDRVPLLRWAQRRLDGQGELAWISDADDEMV
                     SHLEGEGYEVVHIDRSYRWDLTGDLPEPVLPDGWALRAVLGENEANERRRASHAAFES
                     TMPEAMHLQRYLDFMRSPAYVPERDLVAVAPDGRIGSFMVWWGDRTGVAQIEPFGTHP
                     DFHRRGIGRALLYHGLREMKAAGMHTCRVVTDDDREATSFYEGVGFRDVGRLHWWARR
                     "
     gene            765..1613
                     /locus_tag="VFZ80_06620"
     CDS             765..1613
                     /locus_tag="VFZ80_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6287141.1"
                     /translation="MSDAYRPLEGKVALITGGSRGLGAATARHLARWGVRVIITYRQN
                     SEAAQQVVSSCSELTPRARAIPMDLLEEESVREGFASLGEERLDFLIANAAATALKPL
                     LALKMHQIDKTFAISVRHFILMTQLAFPLLEKTGGRVIAVSGADTVGYIPTHGLLAAA
                     KSSMETLIRYLAAELGPSGVTAMGVLPGYIDTDSIQMMTGPLYELLKEAEIETHPLRE
                     AATPEQAAEAVALMCLPEARWLNGQVVHNDGGGLYAMQGRFFQMAAKAKGASPDESSP
                     IIPGRW"
     gene            1649..3985
                     /locus_tag="VFZ80_06625"
     CDS             1649..3985
                     /locus_tag="VFZ80_06625"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEX6287142.1"
                     /translation="MFTGYKGRVRRPRDVIMAVVGTALTIWAVAAVDRLPDWAISLTE
                     LVATAPDWVTSLLAVGYLLSLLYSVVVLGGVVLRAKTRNGALRDVILVVTISVALAVL
                     LSWLVNRGWPYFLPEIDLENAEPRFPVTRVTVVTGLLVVVSTYLTRPLRRFGWLAILI
                     TAIAAVGLGYGSPIHVMGSFGLGLAAAGLLQLAVGTPRGYPAPDAVAQGLAGLGVPST
                     ELKASADQTWGLVRFDATGTDGTAIDLKVYGRDAFDSQLAAKLWRTLIYKEMSRTISY
                     SRLQTVEHEALVSLMAARRGVSVPDLLAVGNASAEIALIAFAHAGEPLAVLEPEAVSD
                     DFLVAVWRQVGTMHGGSVSHGRLNTKTVSIANGSPVFTDFDLGSLAPGDDDRAGDTVE
                     LLFSLSLLVGHERAVRTALDGLGRDSLIAVLPYLQVPAVSATSRRQVDKPKQIVNALR
                     DEILEMTGATAPPRVELRRVTLKSLGTVLLLLLVAYALLPLLTEVDYAQIWDVLQSAD
                     WMLLVLGVIVGHLQFFPSATATMFAVPATLPFWPLVTLQTASQFISLAIPSSAGRVAM
                     NAAFLHKFGVPVTVALAQGAIDGFSGFLVQVLILLVVLLTGDVDLGLSIDPSDLPWLI
                     ILAVVVGVVALAVLLVLRVQKLREKVLPVVKQAWGALMVVLRQPSRALGLLGSNFVYW
                     NTLGLTLWVLLDAIGSPIGYGSALFVAVGTSLFAGFMPVPGGVGVAEATMVALLATFG
                     VDQAAAFAATAVYRAATFYLPAIEGMFGTRWLRSNDYI"
     gene            complement(3982..4683)
                     /locus_tag="VFZ80_06630"
     CDS             complement(3982..4683)
                     /locus_tag="VFZ80_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287143.1"
                     /translation="MSIEGLQAFALGGLAQSSLLLAGLVVYWVKPTKRVIGALAGYGA
                     GALIAAVAFDLVPEAEELLNWELAIWMLGGALVFVVSDRIVESKFGEGDSAGPLGIVV
                     GSVVDGIPESIIFGIQIGAGSPIGAAFIAAVFISNIPQALAPSASLAESGWPRARTTA
                     MWSIVVLACAVTAWLGYLLGSAGDATGARPAALAAGGLLAMLTDSLMPYAVKEGGSPA
                     GVWTVVGFVTALAMA"
BASE COUNT          818 a         1592 c         1622 g          953 t
ORIGIN      
        1 tcgccctcct ggcgagatca acgtcttcgc ccgccccggt gtcgatcggg tcccgttatt
       61 gcggtgggca caacgtcgtc tcgatgggca aggggagctg gcgtggatca gcgatgcgga
      121 tgatgagatg gtgagtcacc tggagggaga gggctacgag gtggttcaca tcgaccgctc
      181 atatcgatgg gacctcaccg gtgatctgcc cgagcctgtg ttgcccgatg gttgggctct
      241 gcgtgccgtc ctcggtgaga acgaggccaa cgagcgacga cgcgcctctc acgccgcctt
      301 cgaatcgacc atgcccgagg cgatgcactt gcagcggtat ctcgacttca tgaggtcacc
      361 ggcgtacgtg cccgaacgcg atctggtcgc cgtcgccccc gacgggcgga tcggctcctt
      421 catggtgtgg tggggcgaca ggacaggggt ggcccagatc gagcccttcg gcacccatcc
      481 cgactttcac cgtcggggca tcggccgggc cctcctctat cacggactgc gtgagatgaa
      541 ggcggcgggg atgcacacct gccgggtagt gaccgatgac gacagggagg ctacgtcctt
      601 ctacgagggg gtcgggttcc gcgacgtggg tcgtttgcac tggtgggcac gccggtagga
      661 gtccccctgg ttctgtgccc gggggtgtgt gagcctgctg acggcctact gaagtggagg
      721 gactttgatc ccccgacagg ggacccctcg atatggtcac cggcatgtcg gacgcatatc
      781 ggcccctcga gggcaaagtc gccctgatca cggggggcag ccgtgggctg ggggcggcca
      841 cggcgagaca tctggcccgt tggggggtcc gggtgatcat cacctatcga cagaactccg
      901 aggcggcaca gcaggtcgtg agctcgtgct cggaactgac acccagagcg cgcgctatcc
      961 cgatggatct gctcgaggag gagagcgtga gagaaggctt cgcctcactc ggcgaggagc
     1021 ggctcgactt cctgatcgcc aatgccgccg ccaccgcgct gaagccgttg ctggctctga
     1081 agatgcatca gatagacaag acattcgcca tctcggtccg tcatttcata ctcatgaccc
     1141 aactcgcctt tccccttctc gagaagaccg ggggacgcgt catcgccgtc tccggcgccg
     1201 acacggtggg ttacataccg acccacggtc tcctcgccgc ggccaagtcg tccatggaga
     1261 ccctcatacg atatttggcg gctgagctcg ggccctccgg ggtgaccgcg atgggcgtcc
     1321 ttcccggata catcgacacc gactcgatac agatgatgac cggaccgctc tacgagcttc
     1381 tcaaggaggc cgagatcgag acccatccgc tccgggaggc ggccacaccg gagcaggcag
     1441 ccgaggcggt ggcgttgatg tgtcttcccg aggcgagatg gctcaacggc caggtcgtgc
     1501 acaacgatgg cggaggtctc tacgccatgc agggcaggtt cttccagatg gccgcgaagg
     1561 ccaagggggc gagccccgac gagagctctc cgatcatccc cgggaggtgg tgatcagagc
     1621 tcttagcgcc cccctcgcgt cgtacttcgt gttcaccgga tataagggtc gggtcagacg
     1681 cccacgcgac gtcatcatgg cggtcgtcgg caccgccctg accatatggg ccgtcgccgc
     1741 ggtcgacaga ctgcccgatt gggcgatttc tctcaccgag ttggtggcca ccgctccgga
     1801 ttgggtcacg tcgctcctgg ctgtcggtta cctgctcagc cttctctact ccgtggtcgt
     1861 gttgggtggg gtggtcctgc gagcgaagac acgaaatggg gcattacgcg acgtcatcct
     1921 cgtggtcacg atctccgtgg ctctagcggt cctcctttcc tggctggtca acagagggtg
     1981 gccctacttc ctgcccgaga tcgacctgga gaacgccgag ccccggttcc cggtgacacg
     2041 ggtgacggtc gtgaccgggc ttctcgtggt ggtctccacc tacctgacgc gcccgctgcg
     2101 caggttcggc tggctggcga ttctgatcac ggcgatagcc gccgtgggtc tcggctacgg
     2161 cagtcctata catgtgatgg gatcgttcgg cctcggtctc gccgccgccg gtctgctcca
     2221 gttggccgtg ggcaccccga ggggttaccc ggcgccggac gccgttgccc aaggcctggc
     2281 gggcttggga gtccccagca ccgagcttaa ggcgtcagcc gaccagacct ggggcctggt
     2341 ccggttcgac gccacaggga cggacggtac ggccatcgac ctcaaggtct atgggcgcga
     2401 tgccttcgat tcacagttgg cggcgaagct gtggcggacc ctcatctaca aagagatgag
     2461 ccggacgatc agctactcgc gtctgcaaac cgtcgagcac gaggccctgg tctcgctgat
     2521 ggcggcacgg agaggggtct ccgttcccga cttgctggca gttgggaatg cctcagccga
     2581 gatcgccctc atcgcgtttg cacacgcagg tgagccgctg gccgttttgg agccggaggc
     2641 agtctccgac gatttcctgg tggccgtgtg gcgacaggtg gggacgatgc acgggggatc
     2701 tgtcagccac ggccgtctca acacaaagac cgtttcgatc gcgaatggct ctcctgtctt
     2761 caccgacttc gaccttggat ccttggcacc gggcgatgac gatcgtgccg gcgacaccgt
     2821 ggagctgctc ttctctctga gcctgttggt gggtcacgag cgtgccgtgc gaactgcttt
     2881 ggacggtttg gggcgagact ctctgatagc cgttctcccc tacctccaag ttccggcggt
     2941 gagcgccact tcgagacggc aggtcgacaa acccaagcag atcgtcaacg ccctgcggga
     3001 tgagattctc gagatgaccg gcgcaacggc tccccctcga gtcgagctga gaagggtcac
     3061 tctcaagagc ctggggacgg tcctccttct cctcctcgtc gcctatgccc tattgcctct
     3121 tctcacagag gtcgactacg cccagatctg ggacgttctc cagagtgcgg attggatgct
     3181 cttggtcctc ggggtgatcg tagggcactt gcagttcttc ccctccgcca ccgcgacgat
     3241 gttcgccgta cccgcaaccc tcccgttctg gccgctggtg accctgcaga cggcgagtca
     3301 gttcatcagc ctggcgatac cgagctcggc aggccgggtc gccatgaatg ccgccttcct
     3361 gcacaagttc ggtgtccccg tcaccgtggc cctcgcccag ggggcgatcg atggcttctc
     3421 gggatttctc gtccaggtgt tgatcctcct ggtggtcctg ctgaccggag acgtcgatct
     3481 gggcttgtcg atcgacccgt ccgacctgcc gtggttgatc atcctcgccg ttgtggtcgg
     3541 agtcgttgcc ctcgctgtct tgttggtcct tcgggtccag aagctgaggg agaaggtcct
     3601 gcccgtcgtc aaacaggctt ggggcgctct gatggtggtc ctgcggcagc cgtctcgggc
     3661 cctgggcctg ctcggaagca acttcgtcta ttggaacacc cttggactga cactgtgggt
     3721 gctgctcgat gccatcggct cgccgatcgg atatggcagc gcactgttcg ttgcggtggg
     3781 gacgagcctc ttcgccggat tcatgcctgt gccgggaggc gtcggagtcg ccgaggcgac
     3841 catggttgca ttgctcgcca ctttcggagt cgatcaggca gctgcgttcg ccgccaccgc
     3901 cgtgtatcga gcggcgacgt tctatctccc ggcgatcgag gggatgttcg ggacgcggtg
     3961 gctgcgcagc aacgactaca tctaggccat ggccagggcg gtcacgaacc cgacgacggt
     4021 ccacactccc gccggtgacc ccccttcctt gaccgcataa ggcatcaggg agtcggtcag
     4081 catcgccagc agccccccag ctgcgagagc agcgggtcgc gccccggttg catcccctgc
     4141 gcttccgagc aggtagccga gccatgcggt gaccgcgcag gcgagaacca cgatgctcca
     4201 catcgcggtc gttctggcgc gcggccaccc ggactcggcg agtgaggcag acggcgccag
     4261 ggcctgggga atgttggaga tgaaaacggc tgcgatgaat gctgcgccga tagggctgcc
     4321 ggccccgatc tgaatgccaa agatgatcga ctccggaatc ccgtcgacga ccgatccgac
     4381 gacgatgccc agtggccctg cgctgtcgcc ttcaccgaac ttcgattcga cgattcggtc
     4441 ggagacgacg aaaacgagag ctcccccgag catccagatg gcgagctccc agttgaggag
     4501 ctcctcggcc tccgggacga ggtcgaacgc caccgccgca atcagcgcac ccgccccata
     4561 gccggcgagc gccccgatca cacgcttggt cggcttgacc caatagacga ccagcccggc
     4621 gagcagcagc gacgattggg ccaacccgcc gagagcgaat gcttggagac cttcgatact
     4681 cacgagcgct gtcggtctgg tcccatccgc tcgggatcat acggtcggga tcatacggtc
     4741 ccgtatctct gcggcctcgc cccctccgcg ctcgacacct agtgtccaca ccgaaggacc
     4801 gaatcgcggt cacgaggcga tcgaggagga agtgtggcta cacgagacgg gagcagcggc
     4861 tgggcggcgg cctggaccat ctacgcagct gtctggatgt ggattcttgg attcatgcat
     4921 ggcatcgccg gctttgccgg gatcgtcgaa gacgaggtcc tggtggcgac acccaactac
     4981 ctctt
//
LOCUS       DASUTA010000333         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1783812, whole genome shotgun sequence.
ACCESSION   DASUTA010000333 DASUTA010000000
VERSION     DASUTA010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783812"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VFZ80_06635"
     CDS             complement(<1..559)
                     /locus_tag="VFZ80_06635"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF024489.4"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEX6287144.1"
                     /translation="MELISPDTFINRELSWLDFNKRVFALTGREDFPLLEKAKFLAIW
                     SSNLDEFFQVRVAGLKEQVAHGLRSATPDGLTPGQQLAAIRDEVDAQYQEISKRFLSE
                     IAPALAREGIVFSDYSTLDDEDREFLDKQFADLVFPVVTPLAVDPAHPFPYISNLSLN
                     LAIFVRNPETTLTRFARVKIPPVLPR"
     gene            complement(598..1557)
                     /locus_tag="VFZ80_06640"
     CDS             complement(598..1557)
                     /locus_tag="VFZ80_06640"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX6287145.1"
                     /translation="MSLRAGKTSSEVAASPVERIRIRLDGLIEIDYLTTLCGDDDHFS
                     PGKRGHQEDPNGTMATMSAVRPMGDRACQPDQDSEDCASERLAPRLLQTLLDRASNPG
                     PGPIEAIPVLARYPWRMPKIRAASIDEHKELTRRALLDAAYHVIDDAGTAEVPLGEIA
                     LLAGVGRTTFYDYFDDRDDVIASLVEEKLPGVIADLIEAVPDDLATTERLARLASRTV
                     EFVATDRVFGVILHREAGRMGTEAQQRIRRSHSQLAIEMASIYQKGVAEGVFVAMPDY
                     LAGRLIQDTIMSAARALIDDESRIAEVTAGVERFLLGGLGGNR"
     gene            1573..1878
                     /locus_tag="VFZ80_06645"
     CDS             1573..1878
                     /locus_tag="VFZ80_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX6287146.1"
                     /translation="MTAGRSFAELSPHIATEVVADWRNRAACSGYPNTLFFPASEGAD
                     EKSVEKAKGVCAVCPVTEDCLQYALETNQRSGIWGGTTEKERKSLRRKWLAERRRSA"
     gene            1954..>2135
                     /locus_tag="VFZ80_06650"
     CDS             1954..>2135
                     /locus_tag="VFZ80_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004931998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX6287147.1"
                     /translation="MSKQSDYVMRTVEERGIRFIRLWFTDVLGFLKSVAISPAELEVA
                     FDEGMTFDGSSIDGYAR"
BASE COUNT          402 a          640 c          703 g          390 t
ORIGIN      
        1 accggggcag gaccggcggg atcttgaccc gggcgaacct ggtcaaggtg gtctcgggat
       61 tcctgacgaa gatcgccagg ttgagcgaga ggttcgagat gtacgggaac ggatgtgcgg
      121 gatcgacggc caggggggtc actaccggga agacgagatc ggcgaattgc ttgtccagga
      181 actctcggtc ttcgtcatcg agcgtcgagt agtcgctgaa cacgatgcct tcccgggcga
      241 gggctggcgc gatctcggag aggaaacgct tcgagatctc ctgatattgg gcatccacct
      301 cgtcccgaat ggccgccagc tgctgacccg gcgtgagacc gtcgggcgtg gcagagcgga
      361 ggccgtgagc cacttgctct ttgagcccgg cgacacgcac ctggaagaac tcgtcgagat
      421 tcgatgacca aatggcgagg aatttcgcct tctccaggag cgggaagtcc tcccggccgg
      481 tcagcgcaaa gacgcgtttg ttgaaatcga gccatgacag ctcgcggttg atgaaagtgt
      541 cgggggagat gagttccatc gtttcagtca tgatcgtcga ccacgattgc caaacggtta
      601 acgatttccg ccgaggccac ccaggagaaa cctctccact cccgcggtga cctcggcgat
      661 acgggactcg tcgtcgatca gggcacgtgc cgccgacatg atcgtgtcct ggatgagacg
      721 ccccgccaga tagtcgggca tcgcgacgaa gacaccctcg gcaacaccct tctggtagat
      781 cgacgccatc tcgattgcga gttgggaatg ggatcgtcgg atccgctgct gagcctctgt
      841 tcccatacgc cccgcctctc tgtggaggat gactccgaac acgcggtcgg tggccacgaa
      901 ctcgacggtc ctcgaggcga gacgggccaa acgctcagtc gtggcgaggt cgtctggtac
      961 ggcctctatc aggtcggcga tgaccccggg gagcttctcc tcgaccagtg acgcgatcac
     1021 gtcgtcgcgg tcgtcgaagt agtcatagaa ggtggtccgg cccacaccgg cgagcagcgc
     1081 gatctccccc agcggcacct cggcagtccc ggcgtcgtcg atgacgtggt acgccgcatc
     1141 gagcagagcg cgcctcgtga gctctttgtg ctcgtcgatc gaggcagcgc ggatcttggg
     1201 catccgccaa gggtatcggg cgagaacggg aatggcttcg atcggtccgg ggccggggtt
     1261 gcttgctcgg tcgaggagag tctggagtag acgcggagca agccgctcac tggcgcagtc
     1321 ctcggaatcc tgatcgggtt ggcaagcgcg atctcccatc ggcctgaccg cgctcatcgt
     1381 ggccatcgtc ccgttgggat cctcctggtg cccgcgttta ccgggcgaga agtggtcgtc
     1441 atcaccacag agagtggtga ggtagtcgat ctcgatcagc ccgtccagtc tgattcggat
     1501 gcgctcgacg ggggaagcag cgacctccga gctcgttttg ccagcgcgca atgacaccac
     1561 caatatggcc tggtgaccgc aggtcgaagc tttgcggagc taagcccgca tatcgcaacc
     1621 gaggtggttg ccgactggag gaaccgcgca gcctgctcgg gttaccccaa cacgctcttc
     1681 ttcccagcct ccgagggagc cgacgagaag tcggtggaga aggcgaaggg cgtctgcgcc
     1741 gtctgtcccg tgacggagga ctgtctgcag tacgccctgg agaccaacca gcgctccggc
     1801 atatgggggg gcacgacgga gaaggagcgc aagtccctcc gtcgcaagtg gctcgccgaa
     1861 cgacgccgca gcgcctagag cccgccccct atttgcgaac ccggggtacg tggggtcata
     1921 tatggctcaa atcagctcga gttcggtgga cagatgagta agcagtccga ctatgtgatg
     1981 cgaaccgtcg aggagcgggg tatccgattc atccggctct ggttcacaga cgtcctcggc
     2041 ttcctcaagt cggtggccat cagccccgca gaactggaag tcgccttcga cgaggggatg
     2101 accttcgacg gctcctccat cgacgggtat gcccg
//
LOCUS       DASUTA010000334         6832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_617659, whole genome shotgun sequence.
ACCESSION   DASUTA010000334 DASUTA010000000
VERSION     DASUTA010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6832
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617659"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="VFZ80_06655"
     CDS             complement(<1..148)
                     /locus_tag="VFZ80_06655"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6287148.1"
                     /translation="MTLGRPLLLEGEVGVGKTELAKALAEVFDRELIRLQCYEGIDTN
                     QALYE"
     gene            complement(266..517)
                     /locus_tag="VFZ80_06660"
     CDS             complement(266..517)
                     /locus_tag="VFZ80_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287149.1"
                     /translation="MTAQDETTAEVLQSVWMVNHDHFELDEDCSPTKPCRNMRGVSFD
                     GYAEPGSDIGAAWRAQLAREGKLDKSAGTIRDLVLGKDE"
     gene            complement(591..1490)
                     /locus_tag="VFZ80_06665"
     CDS             complement(591..1490)
                     /locus_tag="VFZ80_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX6287150.1"
                     /translation="MYDIAMTVTACLQAGTRADVAWVVGAEGLAVPDWSDAVVFTPGG
                     GRTGSLLEGAIDGKLIEQAGRLSKGRLVDLEITQVDVLIAELPSTGSVRCILAPADTL
                     PAELWSLAVGRERFGLVLDLDGDEVVDVSVYSQLTVSEADRDVRESFADRPSGSVVTD
                     SRVISVFEAVPQMVIVGGGPIADALAELAPLVGWQAQIAASRDMATGLITPLSSHDKV
                     VIAAHDLELAGSGLTAALESSCGYIGSVGSRKMQADRADWLAYRGITDLSRVHGPAGL
                     DIGAANPGEVAVAIVAEAIASGR"
     gene            1551..1859
                     /locus_tag="VFZ80_06670"
     CDS             1551..1859
                     /locus_tag="VFZ80_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX6287151.1"
                     /translation="MRSQLAMRNKWREEGKDVAVATVIKVHGSAPRPEGSKFLVSSAG
                     DMEGSVSGGCVENDVFLHAQEVLETGKPKKVTYGIADEDAFEVGMSCGGTIDVFIEPW
                     "
     gene            1870..2643
                     /locus_tag="VFZ80_06675"
     CDS             1870..2643
                     /locus_tag="VFZ80_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX6287152.1"
                     /translation="MTDLNQAIEELLDAELFGSVVTVVDGPSVGEKAVVDSNGKVIAG
                     SVPAAISSDVLEDAITLMSHEQHRTLDYGDHSVFIETLAPPPDLIIFGAVHIGQALTS
                     FGTQMGYRVTVVDSRAPFVTEDRFPAAHKLLVGWPRDLMDQLTFDRRTWVVVLSHDPR
                     HETPPLEAALKADTRYIGAMGSRRTHDLRVERLQELGFGADDIARIHSPVGLDIGAET
                     PQEVAVSILAEMTLVRYGAGTGVSLRGVEGRVHKQRGNR"
     gene            2693..3364
                     /locus_tag="VFZ80_06680"
     CDS             2693..3364
                     /locus_tag="VFZ80_06680"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX6287153.1"
                     /translation="MFQGENWEWLALLAIAAALGALVGGERESRGHPAGIRTQALVAL
                     AAALLTRVGMTGFIGPAVDLSRVASQVVTGIGFIGAGVILKHRGTVTGLTTAATLFLS
                     AGIGVAVGAGLVVPAATATGIALIVIYGLRLLKPWLRSQVSRTVQVDYLQGQGTFGPL
                     VRTIQDSGATIQDLRVEDGTSADGESLRHVSIQMLTKQDADFDALLQSFLARPEVRDI
                     AIFER"
     gene            complement(3383..3820)
                     /locus_tag="VFZ80_06685"
     CDS             complement(3383..3820)
                     /locus_tag="VFZ80_06685"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6287154.1"
                     /translation="MRFQQTKFLAGDVESLTEFYVDALDCEVVVNPQTVDQVVSRAVG
                     VPDATIKLSILRPPGQGDHGPVLELYSVDGSRPDGWEYHPGSGQIAFEVEDLEAGIGR
                     VIAAGGSLLGEVVEWTGPSGAVAKFVYLNDPEGNIIDLFVRVG"
     gene            3868..6447
                     /locus_tag="VFZ80_06690"
     CDS             3868..6447
                     /locus_tag="VFZ80_06690"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6287155.1"
                     /translation="MLLEREGQLAALRDHLDAAGVGDGSLVLVAGEAGAGKTSLIDAF
                     VESLDHKTLVIQGACDPLTTPRPLGPLHDFAADSGSGLSDLAVDAADPFQMFESVLDR
                     LKSTVRPILMVIEDIHWADDATLDFLRFVGRRVADAKSVVVCTYRDDEVGPDHPLRPV
                     LGQLIPLPTTDRLIVPPLTIEAVRRLVSGHEVDPDALIAITDGNAFFVTEVLAAGYVV
                     PDTVQEAVLSRVARLDESPRRVVEAVSIAPRSLDVEHAMAMVGGAIGHVDAALAAGVL
                     IGDGRSLRFRHELARSSVEDSLPPGRRLSLHLRMLRLLDEDPNPDLARMAHHAIRAGD
                     SERIIEHAPQAAREASRRGAHREAISFLEAAIKEIDRSGQDDLAARLRIDLAEEYKTI
                     DQTRKAIGVAEEAVAYFREAREVGELGLALTTLSRLRWRVNEDRLGMEVLNEALSLPY
                     DDSENRVLAEAYAQLAYLLMLGRHGSEGRRALTTARAIAGEDITTDLAWDMDLLDSTL
                     RIVMGDSQQGVDGLTESLWSARKLGDPGRVALALSMLGSGGGEVRLYPESVAALKESI
                     EHGLAHDEDYSVAYSRSWLARIAHERGQWDEAVRHAELVEATTTQVESIAYVTAMSAL
                     GRVRVRRGDPGGISLLDELADEGAHHELQHIWNTLCGRSEYHWLRGRPESGLDLLKPA
                     FERALDTESEWARGEIGYWMWKAGEIDGPPEGAAEPFALQMSGRWREAAQTWREIGCP
                     YEVGLSLAEGDVDAMKEALEVFDSLEARPMADRVRSQLREIGVESIPRGPLRSTRENP
                     AGLTNRQVEVLELMADGLANAEIAEKLFISKKTVEHHVSAIYTKLGVDSRTKATATLH
                     QQK"
     gene            6552..>6832
                     /locus_tag="VFZ80_06695"
     CDS             6552..>6832
                     /locus_tag="VFZ80_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020514578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEX6287156.1"
                     /translation="MPRYIVERDFPQGLEIPSTPDGQKAMLGVVGNNATEGVTWVQSF
                     VTDDHTKTFCIYDGPSPEAIRRAAGLNSLPVNAISEISVLDPYFYVGASS"
BASE COUNT         1272 a         2200 c         2164 g         1196 t
ORIGIN      
        1 actcatagag ggcctgattg gtgtcgatgc cctcataaca ctggagacgg atcagttccc
       61 tgtcgaacac ctccgccaga gccttcgcca gttcggtctt gcccacgccg acctcgccct
      121 cgagtagcaa aggccgtccc agggtcatgg cgacgtagac cgccgtagcc aggccgcgat
      181 cggccagata gtgacttgct cgcagcaggt cggtcagttc ggcgacattt gacggcgtcg
      241 cccgggaacc gggtgcagag ctcagctact cgtccttgcc gagaacgaga tcgcggatcg
      301 tcccggcgga cttgtcgagt ttcccctcac gcgccagttg tgcccgccac gcggcaccga
      361 tgtcgctgcc cggctcggcg tagccatcga agctcacgcc tcgcatgttg cgacatggct
      421 tggtcgggct gcagtcctcg tcgagctcga agtggtcgtg attcaccatc cacaccgatt
      481 gcagcacttc ggccgtggtc tcatcctggg cagtcatcac gagctcctcg tcggaaaaca
      541 gctgagcgct cagtctaatg gcgacggcgt gttggctcca tgagcgagcc tcatctaccg
      601 gaggcaatcg cctcggccac aatggccacg gccacttccc cggggttagc tgccccgatg
      661 tcgagacctg ccggcccgtg aacccgactc agatcggtga ttcccctgta cgcgagccaa
      721 tcggcacgat cggcctgcat cttgcggctt cccacagacc cgatgtagcc gcagctgctc
      781 tccaacgcag ccgtcaagcc cgaccccgcc agctcgagat cgtgagcggc gataacgacc
      841 ttgtcgtggc tggagagtgg agtgatcagc ccggtggcca tgtctcgtga agccgctatc
      901 tgtgcctgcc agcccacgag tggagccaac tcggcgagtg cgtcggcgat ggggcccccg
      961 ccaacgatca ccatctgagg gacggcctcg aagactgaga tgactctgga atcggtgacg
     1021 accgacccac tggggcgatc ggcaaaggat tcccgaacat cccgatcggc ctccgagacc
     1081 gtgagctgtg aatagacact gacgtcaacc acctcgtcgc cgtcgagatc cagcacaagg
     1141 ccgaaccgct ctcgcccgac ggcgaggctc cacagctcgg cgggcaaggt gtcggctgga
     1201 gccagaatgc accgcaccga gcccgtcgag ggcagttcgg cgatcaggac gtcgacctgg
     1261 gtgatctcca gatccaccaa ccggcccttg ctcagccttc ccgcctgttc gatcagcttc
     1321 ccatcgatag cgccttcgag gagcgagccg gtcctgcccc cgccgggggt gaacacgacc
     1381 gcgtcggacc aatcgggcac tgcaagccct tcggcgccca ccacccaggc gacatcggct
     1441 cgggtaccgg cttggagaca tgcggtgacc gtcatggcta tgtcgtacac ggaagcgact
     1501 ctacggccgg cccgcgacat cgggcccgtc ccacgaccgt tcaggttgag atgagaagcc
     1561 agctcgcaat gcgcaacaaa tggagagaag agggcaaaga cgttgcggtg gccaccgtca
     1621 taaaggtgca cggcagcgca ccgcgccccg agggctccaa gttcttggtg tcatcggccg
     1681 gtgacatgga gggctcggtg agcggcggct gtgtcgagaa cgacgtcttc ttgcatgccc
     1741 aggaggtcct cgagaccggc aagccaaaga aggtgaccta cgggatcgcc gatgaggatg
     1801 cattcgaggt gggcatgtcg tgtggcggca cgatcgacgt cttcatcgag ccctggtagc
     1861 ccctcgcaag tgactgatct caaccaggcc atcgaggaat tgctggatgc cgagctgttc
     1921 ggctccgtcg tcaccgtggt cgacggcccg agtgtgggcg aaaaggcggt ggtcgactcc
     1981 aacggcaagg tgatcgcggg ctccgtgccc gcagcgatct cctcagacgt cctcgaggac
     2041 gccatcaccc tgatgagcca cgagcaacac aggacgctcg actacgggga tcactccgtg
     2101 ttcatcgaga ccttggctcc cccaccggac ctgatcatct tcggcgcggt gcacatcggt
     2161 caggctctca cctctttcgg gactcagatg ggataccggg tcaccgtcgt cgactccagg
     2221 gctccgttcg tcacagagga ccgttttccg gctgcccaca agctcttggt cggctggccc
     2281 cgcgacctga tggaccagct cacattcgac cggaggacct gggtggtagt cctgtctcac
     2341 gacccccgcc acgagacccc acccctggag gcggccctga aagccgacac ccggtacatc
     2401 ggggccatgg gcagccggcg cactcacgat cttcgggtcg agcgcctcca agagctcgga
     2461 ttcggcgccg acgacatcgc ccgcatccac tctccggtgg gtctcgacat cggcgccgag
     2521 actccgcagg aggtcgcggt gtcgatcctc gccgaaatga ccctggttcg ttatggagcc
     2581 ggcaccgggg tgtcgctgcg aggtgtcgag ggcagggttc acaaacagcg cggcaacagg
     2641 taactccagc atcccgtttg ggtctcgtgg gttccgggga aaaagctgtc ccatgttcca
     2701 gggagagaat tgggaatggc tggcgcttct cgccatcgct gccgcgctcg gcgctctggt
     2761 cggcggcgag cgggagagtc ggggccatcc ggcggggatc aggacccagg ccctggtggc
     2821 tctggccgcc gccctgctga ctcgagtggg gatgaccgga ttcatcggtc ccgccgtcga
     2881 cctgagtcgc gttgcctctc aggtggttac cggcatcggg ttcatcggcg ccggcgtgat
     2941 cttgaaacac cgcgggacgg tcacggggct gactactgcg gccactctct ttctcagtgc
     3001 cggtatcggc gtcgccgtgg gcgccggcct ggtcgttcct gccgcaactg ccaccggcat
     3061 cgccctgatc gtcatctacg ggctgcgtct cctcaagccg tggctgagaa gccaggtttc
     3121 ccgcacggtg caggtcgact acctgcaggg gcagggcacc ttcgggccct tggtcaggac
     3181 catccaggac tccggggcga ctatccagga tctccgtgtc gaggatggca cgtcggccga
     3241 tggtgagtcg ctccgacacg tctcgatcca gatgctgacc aaacaggacg ccgacttcga
     3301 tgcactgctc cagtccttcc tggctcggcc cgaggttcgt gacatcgcca tcttcgagcg
     3361 ctgagtccga aaggatctgg actcagccga cccggacgaa caagtcgatg atgttgcctt
     3421 cggggtcgtt cagatagacg aacttcgcca ccgccccgga cggtccggtc cactcgacaa
     3481 cttcaccaag caggctgccc ccggccgcga tcacccgccc tatacctgcc tccagatcct
     3541 ccacctcgaa tgcgatctga ccactgcccg ggtgatactc ccacccatcc ggtcgggatc
     3601 catcgaccga atacagctcg agaaccggcc cgtggtcccc ttgtcccggc ggtcgcagga
     3661 tcgagagctt gatcgttgca tcggggaccc ccaccgcccg cgacaccacc tgatccaccg
     3721 tctggggatt gaccacgacc tcgcagtcga gagcgtcgac gtagaactcg gtgagacttt
     3781 cgacatcgcc ggccaggaac ttcgtttgtt ggaaccgcac ggagtcgacc ataccgctca
     3841 gcgggagggc cggatacgct cattgtgatg ctgttggagc gagagggaca gttggcagcg
     3901 ctgagagacc atctcgatgc tgccggcgtc ggagacggat cgctggtgct cgtcgccggc
     3961 gaagcgggtg ccggcaagac atctctcatc gatgcgttcg tcgaatcgct agaccacaag
     4021 acgctcgtca tccagggcgc gtgcgatccg ttgaccaccc ctcgaccgct gggcccacta
     4081 cacgacttcg ccgccgactc cggctccggt ctgtccgatc tcgctgtcga tgccgccgac
     4141 cccttccaga tgttcgaatc ggtgctcgat cgcctcaaga gcacggtccg gcccatcctc
     4201 atggtcatcg aggacatcca ctgggccgac gacgcaactc tggatttcct gcgattcgtg
     4261 gggcgacgtg tcgcagacgc caagtctgtg gtggtctgta cctatcgcga cgacgaggtc
     4321 gggcccgatc accctcttcg gccggtcctc ggacaattga tcccgttgcc gaccaccgac
     4381 cgtctcatcg tgcctccact gacaatcgag gcggttcgac gactggtgtc gggacacgag
     4441 gtggatcccg atgcattgat tgcaataacc gacgggaacg ccttcttcgt gacggaggtg
     4501 ctggcggccg gctacgtggt gccagacaca gtccaggagg cggtcctgtc acgagtcgcc
     4561 cggctcgacg agagcccgag acgggtggtg gaagcggtgt cgatcgctcc gagatcactg
     4621 gacgtcgaac acgcaatggc aatggttggc ggtgcgatcg gccacgttga cgcggcactc
     4681 gccgccggag tgctcatcgg tgacggccgc tcgcttcgat ttcgccatga gctggccagg
     4741 tcgtcggtag aggactctct gcctcccggc agacggctca gcctgcatct gagaatgctc
     4801 cggctcctcg acgaggaccc gaacccggac ctggcccgaa tggctcacca cgcgattcgc
     4861 gcaggtgaca gcgaacgaat catcgagcat gccccacagg cggcacgcga ggcttcgaga
     4921 cgaggggcac atcgcgaggc gatctcattc ctcgaggcgg cgataaagga gatcgatcga
     4981 tcggggcagg acgatctcgc cgccagactg cgcatcgatt tggccgagga gtacaagacc
     5041 atcgatcaga cacgtaaggc gatcggggtc gccgaggaag ccgtcgccta cttccgcgag
     5101 gcaagagagg tcggggaact cggactcgcg ctgaccaccc tttctcgtct caggtggcgt
     5161 gtcaacgagg accgactggg catggaggtc ttgaacgagg cactgagcct gccttatgac
     5221 gattcagaga accgggtgct tgccgaggcc tatgcccagc tcgcatacct gctcatgctg
     5281 gggcgacatg gatccgaggg acgacgggcc ctcacaacgg ctcgagcgat cgccggcgaa
     5341 gatatcacca ccgacctggc ctgggacatg gatctgctcg acagcacgct gcgcatcgtg
     5401 atgggcgatt cgcaacaggg cgtagacggc ctcactgaat ctctgtggtc ggccaggaag
     5461 ctgggcgatc cgggaagagt ggcgctagcc ctctccatgc tcggatccgg tggtggagag
     5521 gtgcgtttgt acccagagtc tgtggccgcc ttgaaggagt cgatcgagca cggattggcc
     5581 catgacgagg actactcggt ggcctacagc cgctcatggc tcgcccgaat agcccacgaa
     5641 cgaggacagt gggacgaggc ggtccgacat gccgagctgg tcgaggccac gaccacccaa
     5701 gtggagagca tcgcttatgt caccgccatg agcgctctgg gacgggtacg tgtgcgccgc
     5761 ggcgatcctg gagggatctc gctcctcgat gagctggcag acgaaggggc ccaccacgaa
     5821 ctgcaacaca tctggaacac actctgcggg cgcagcgagt accactggtt gcgaggtcga
     5881 ccggagtcag ggctcgactt gctgaagccg gcattcgaga gggcgctgga cacagagagt
     5941 gagtgggctc gcggcgagat cggctactgg atgtggaaag ccggtgagat cgacggccca
     6001 cctgaaggag ctgccgagcc ttttgcactc cagatgtcgg gccggtggcg ggaagcggct
     6061 cagacctggc gagagattgg ctgcccctat gaggttgggc tctccctcgc tgaaggcgac
     6121 gtcgatgcca tgaaagaggc cctggaagtc ttcgactccc tggaagcacg cccgatggcg
     6181 gaccgtgtcc gctctcagtt gcgggagatc ggtgtcgagt cgatcccacg cggccccctc
     6241 cgctcgacac gagagaatcc ggcagggctg acgaaccgac aggtcgaggt tctcgagctg
     6301 atggcagacg ggctcgccaa cgcggagatc gcagagaagc tcttcatctc caagaagacc
     6361 gtcgagcatc acgtttcagc gatctacacg aaactgggtg tcgacagccg caccaaggca
     6421 acggcgaccc ttcaccagca gaaatagggg cgattcgccc cgatctaggt aaagacaccg
     6481 atgtcatccg agcctcaggg ccgtaccttc gccgccacgc cggaaggacc ggctggtaaa
     6541 aggaggaaca gatgcccaga tacatcgtgg agagagactt cccccagggt ctcgagatcc
     6601 catccacccc tgacgggcag aaggcgatgc tcggtgtagt cggcaacaac gcgaccgaag
     6661 gcgtgacgtg ggtccagtcg tttgtgacgg atgatcacac caagacgttc tgcatctacg
     6721 acgggccttc gccggaggcg atccggcggg cggcgggcct gaactcgctc ccggtgaacg
     6781 cgatctccga gatcagcgtt ctcgacccgt acttctacgt gggggcgtcg tc
//
LOCUS       DASUTA010000335         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1653749, whole genome shotgun sequence.
ACCESSION   DASUTA010000335 DASUTA010000000
VERSION     DASUTA010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653749"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..890)
                     /locus_tag="VFZ80_06700"
     CDS             complement(117..890)
                     /locus_tag="VFZ80_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287157.1"
                     /translation="MSLVHAEYLKVTRRKLFPVMTLILGLLMGFFGVLFFVILPALPE
                     SAGGGAVVPQRPGAFIFGAQQVAGQAWWFAVILATAVFGGELATTSWATSLARESRKV
                     RHIVSKFGVFTVASWLALLIGTAIWTVATYVFADGTGSLAASEWIGFVWKFLVIAAAW
                     TSIGLGAIGLTRSIPSAMAIALGLSFVDSILAPFVELYENVSLTAATNGIFGLAADGP
                     FGALIPGGDMSLAHALSIMAGWTVLGLGLMWWGLQRRDA"
     gene            complement(887..1786)
                     /locus_tag="VFZ80_06705"
     CDS             complement(887..1786)
                     /locus_tag="VFZ80_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6287158.1"
                     /translation="MSTGSPVVRVSNLHKRYGSTQALRGLDFEIPSGQLTGFLGPNGA
                     GKTTTFRAILGLTRPDEGAVEVLGMDVPSGLPEIVKRIGVIVEEPGLIKALSGRVNLE
                     IAADTLGFGHGRIDEQLEFVGLTADAHRKLGDYSKGMRQRLALAAAMLGDPELLILDE
                     PLDGLDPAGQMAFRARLRALSSEGKSVVVSSHDLADIEALADYVVVISQGRLVSQGPL
                     EQLLGDGRIRVVVGDRESAARVLTAKGMSATIDDSGIFVEASDGSEVVKALAEAGIYP
                     SEVKPARETLESVFLGITGEGGL"
     gene            complement(2144..>2372)
                     /locus_tag="VFZ80_06710"
     CDS             complement(2144..>2372)
                     /locus_tag="VFZ80_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2469 family protein"
                     /protein_id="HEX6287159.1"
                     /translation="LPMFSYVVETERRFYLANKVDVEVKNTEGSTYFEVTMTDAWVWD
                     MFRPARFLEKVQVLTFRDVNVEKLEKTELKL"
BASE COUNT          486 a          769 c          724 g          393 t
ORIGIN      
        1 accatcccgg ccgggacctt ccatctcgtg gtgattcccg accacgccgt cgagatgcgt
       61 cgtcactggc atcatggctg ctggcacaag gagttgcgac gcacgctggg taaggctcag
      121 gcgtcccggc gttgaagccc ccaccacatg aggccgagac cgagaaccgt ccacccggcc
      181 atgatcgaga gggcatgtgc cagtgacatg tccccacctg ggatgagggc cccaaacggg
      241 ccatcggcgg ccagaccgaa gatgccgttc gtcgccgcag tcagggacac gttctcgtag
      301 agctcgacga aaggggccag gatcgagtcc acgaaggaca agcccagtgc aatggccatc
      361 gccgagggga tcgatcgggt caagccgatc gcgccgagcc caatcgatgt ccatgcagcg
      421 gcgatcacga ggaacttcca cacgaagcca atccactccg aggcggccag ggacccggtc
      481 ccatcggcaa acacgtaggt ggccaccgtc cagatggcgg tgccaatgag gagggccagc
      541 catgaggcca ccgtgaagac cccgaacttc gagacgatgt gacggacttt tcgcgactca
      601 cgagctagtg atgtggccca cgaagtcgtc gccaactctc cgccgaatac ggcggtggcg
      661 aggatgaccg cgaaccacca tgcctgaccc gcaacctgtt gggcaccgaa gatgaacgca
      721 cccggacgct gagggaccac cgctccaccc cccgccgact cgggcagcgc cggcagtatc
      781 acgaagaaga ggactccgaa gaaacccatg aggagaccga ggatcaaggt catcacggga
      841 aagagcttgc gccgggtgac cttcaggtat tcggcgtgga cgagactcaa agcccaccct
      901 cccctgttat gccgaggaag acggactcca gagtttcccg agccggcttg acctcgcttg
      961 ggtagatgcc ggcctctgcg agggctttga ccacttcgga gccgtcgctg gcctcgacga
     1021 agatgcctga gtcgtcgatg gtggcgctca tgcctttggc cgtgagcacc cgcgcagccg
     1081 actccctgtc accgacgacg acacgtatcc gaccgtcgcc cagcagttgc tccaagggcc
     1141 cttgggagac gagacggccc tggctgatca ctacgacata gtcagcaagc gcctcgatgt
     1201 ctgcgagatc gtggctgctc accacgacgc tcttcccctc gctcgaaagg gctcgaagac
     1261 gggctcgaaa cgccatctgc ccggccgggt ccagcccatc caatggctcg tcgagtatca
     1321 gcagctcggg atccccgagc atggccgcgg ccaaggccaa acgttgtctc attcctttgg
     1381 agtagtcacc gagtttgcgg tgggcatcag cagtgagacc gacgaactcg agttgctcat
     1441 cgatgcggcc atgcccgaaa ccgagcgtgt cggcggcgat ctccagattg acccgaccac
     1501 tgagtgcctt gatcaagccc ggctcttcga cgatgacacc gatcctcttc acgatctcgg
     1561 ggagcccgga cggcacatcc attcccaata cctcgacggc gccctcgtcg gggcgggtga
     1621 gaccgagaat cgcccggaag gtggtggtct tgccggctcc gttgggtccg agaaacccgg
     1681 tcagctgccc tgaggggatc tcgaagtcga gaccgcgaag ggcctgcgtc gacccatatc
     1741 gtttgtgcag attgctgacc ctgacaaccg gcgatcccgt ggacatcgac tcgagagaat
     1801 aggaagcggc ggtcgggtta ccaaagcccg cccggaaccc agtggacctc tattccccac
     1861 cgacgaagac ccacccctat gagacccagc cgagataccc cgagccggcg ggccaaagac
     1921 gctacgtgcc gacgcttggc cgggtcgact gcgtcaatgg gatctcctgg ccctgtgatg
     1981 gtgcgaactt cgatggcgac ccggtcccga ccgtggcgag cgacgagatc gatctctcca
     2041 tcgtcgagga tgacgttgcg cctttcgatg acaaacccac gttcggtgag ccagtgcgcc
     2101 gcgatcgact ccccgatagc gccaacggcg atgcggcgat cggtcagagc ttgagctctg
     2161 tcttctccag cttctccacg ttcacgtcgc ggaatgtgag cacctggact ttctcgagaa
     2221 accgggcagg gcggaacatg tcccataccc aggcgtcggt catcgtgacc tcgaagtaag
     2281 tcgagccctc agtgttcttg acctcgacgt cgaccttgtt ggcgagatag aagcgacgct
     2341 cggtctcgac gacgtaggag aacatgggca gg
//
LOCUS       DASUTA010000336         7592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_874652, whole genome shotgun sequence.
ACCESSION   DASUTA010000336 DASUTA010000000
VERSION     DASUTA010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7592
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874652"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /gene="folK"
                     /locus_tag="VFZ80_06715"
     CDS             <1..970
                     /gene="folK"
                     /locus_tag="VFZ80_06715"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX6287160.1"
                     /translation="RRTIPLTRYAIGLGSNLGDRLEYIVSAVAEISEIGRDWTVSSLY
                     ETEPVGGPEQDPFLNAVMVVDTDLGPRALLERLQDIEQLHGRERKTKWGPRTLDLDIV
                     SSDGGGHRDEKLTVPHPRAAERAFVLAPLAEVWPDAPVGETISAAEALDSIGTEGVDR
                     LSSAWMPPVSPAVPRALVAAQFVMFIAAALALAADGRLPEGEVTVSGVMGALLAMVGL
                     VLAFISSRRLGPSLTPSPVPRKNAQMVMSGPYRYARHPIYGGIFLVLLGTALFLDSVL
                     GVVAAVVAFVFFWAKSTFEERQLRMRFAGYKRYREVVHRRLIPFLI"
     gene            1071..1403
                     /locus_tag="VFZ80_06720"
     CDS             1071..1403
                     /locus_tag="VFZ80_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287161.1"
                     /translation="MAKRSREHRQEIPGYDESRPTREERKLQHRATRHATNQMLHTIE
                     DPEEVVLPQERRTRNNETGNGNAEPAKRRFRVWKTRFWKRRDDYRDEKAALDSNWPVI
                     TPNQLQEE"
     gene            1447..2238
                     /locus_tag="VFZ80_06725"
     CDS             1447..2238
                     /locus_tag="VFZ80_06725"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HEX6287162.1"
                     /translation="MRLVIVGPGRAGQSIAIASERSGHEVVGVLARTPGSTPWPDLGW
                     DIPLPETDIVFIAVRDDDIAEVSEQIAPIVEHAGVVAHLSGFTPVLALHHLQERDIAV
                     GGFHPLVSLPDPQTGAGALAGGFVGIGGDPLAVDMLTHFASTLGMEHFRLDDEARPAY
                     HAAAAAAANFVVASLALSGDLFDSAGIDPRVSRPLVERVVANVFDKGPAASLTGPIVR
                     GDIETVIGHLTAAHEVSDYVGRQFKLMAEATAIRAGREEDLRRWK"
     gene            2235..3116
                     /gene="panC"
                     /locus_tag="VFZ80_06730"
     CDS             2235..3116
                     /gene="panC"
                     /locus_tag="VFZ80_06730"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX6287163.1"
                     /translation="MTRTFDETRGRYEGRVGLVPTLGYFHEGHLRLMDILRGQCDTLV
                     VSLFVNPTQFNETEDFEAYPRDEQRDGDLAAQHGVDLLFAPAVEEVYPGPSVTTVEVI
                     GVTDEMEGRHRPGHFKGVATVVAKLFAGLQPDVAMFGRKDAQQLAVLQAMVRDLRFPI
                     EIVEAPIIREPDGLALSSRNVRLGPKSRYTALAISRGLFQAAEQVDAGERRGDRLEGT
                     VERSMKGLEVDYVKLASQETMKPIGTLDQPAVLAAAAHVDGVRLIDNVAFDMVDGRPV
                     PDRGILLDEPSGLTALP"
     gene            3130..4608
                     /gene="lysS"
                     /locus_tag="VFZ80_06735"
     CDS             3130..4608
                     /gene="lysS"
                     /locus_tag="VFZ80_06735"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX6287164.1"
                     /translation="MSENGDSGEHVLVEGRRQKHEQVIAAGGYPHRFERTHLAADLQE
                     RYSDLGPEQETADRVTVAGRLMLHRSFGKLQFGTIQDVSGVIQLFVDKRVAGDAADEF
                     AEYDLGDWVGASGVIMTTKRGELSVKVDELTLLQKALRPLPDKWHGLVDVEQRSRRRY
                     VDLMVNDEARHVATTRAKIVSELRRQFESRGFIEVETPVMLSQATGALARPFVTHHNA
                     LDLDLQLRIATELYLKRLVVGGLEKVFEIGRIFRNEGIDSTHNPEFTMLEAYQALADY
                     RDIMELVEDVFVAVVEAAVDTSHPTYKGRELSFEKPFRRVSLIELVSEFVGEDVSLDT
                     ESQRLSSLLADRGATVSAGGGVGKLLFELYELAHREIWDPTFVIDFPTEVSPLSRKRD
                     DDPRLTERFELVIAGAEYCNAFSELNDADDQLARFETQAAARNAGDDAAHVIDRDYVE
                     ALEYGLPPTGGLGIGVDRLVMLLTDQAHIREVILFPTLRPEA"
     gene            complement(4690..5676)
                     /locus_tag="VFZ80_06740"
     CDS             complement(4690..5676)
                     /locus_tag="VFZ80_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX6287165.1"
                     /translation="MEDLQSLVGIPRIWEKLNVVEERLLEASTADDPYLTKIAQHLLL
                     AGGKRFRPLLALLAAEFGDSEDGRPVEAAVAVELIHVGSLYHDDVIDEAATRRGATSA
                     NANWTNTVAILAGDFLMARASEVAATHLSQESVRLLAATYAELVEGQTRELQLTDDLT
                     HSLDDYHQVIGGKTASLIRTSARLGAMAAEADKKTVDALSTWAFEVGMVFQIADDALD
                     LVADEDAIGKPAGSDIREGTFTGPVLRAVNGPDGDRVRGLLETGRPYSDDTVAEVIDL
                     VKGGGHIDHALETAKTRLTIAESALATLPKSKPKSILESLGVYLLERVDAVR"
     gene            5818..>7592
                     /locus_tag="VFZ80_06745"
     CDS             5818..>7592
                     /locus_tag="VFZ80_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012184686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX6287166.1"
                     /translation="MFERFTDRARRVVVLAQEEARFLNHNYIGTEHILLGLLNEGEGI
                     AAQALESLDIDLASVRDEVVKIIGQGQQSPSGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLQQLGAELQKVRQTVIQLLSGPGGSEEQQPKAAGSGG
                     RESSSSGSTVLDQFGRNLTQSAREGTLDPVIGRAREIERVMQILSRRTKNNPVLIGEP
                     GVGKTAIVEGLAQRIVSDEVPENLKGKNLYTLDLGALVAGSRYRGDFEERLKKVLKEI
                     KSRGDIVLFIDEIHTLVGAGAAEGAIDAASILKPMLARGELQTVGATTLEEYRKYLEK
                     DSALERRFQPIQVEEPSLTDTIEILKGLKDRYEAHHSVRFTDQAIVSAANLADRYLAD
                     RFLPDKAIDLIDEAGSRMRIHRKSDVPEIVEIDDKIAGVRRDKSEAVGSENYERAASL
                     RDEERSLLLARAEHESGNMDSGWVIDEEEIAEVLAQWTGIPVHRLTEEETARLVHMED
                     ELHKRIIGQHDAIQAVSKAIRRTRAGLKDPRRPSGSFIFLGPSGVGKTETAKALTEFL
                     FGQEDALIQLDMSEYMEKHTVSRLVG"
BASE COUNT         1425 a         2410 c         2408 g         1349 t
ORIGIN      
        1 ccgtcgaacg atcccgttga cccgatacgc catcggactc ggctccaacc ttggagaccg
       61 cctcgagtac atcgtttctg ccgtcgctga gatttccgag attggcaggg actggaccgt
      121 ttcttctctc tatgagaccg agccggtggg tggcccggaa caagacccgt ttctgaatgc
      181 cgtcatggtg gtcgacaccg atctcgggcc acgggctctt ctcgaacgtc tgcaggacat
      241 cgagcagctg catggtcggg agcggaagac gaagtggggg cctcgaaccc tcgacctcga
      301 catcgtgagc tccgatgggg gaggccatcg ggatgagaag ctcaccgtgc ctcatccgag
      361 ggctgcggag agagcgttcg tcctcgctcc tctggccgag gtctggcccg atgcgccagt
      421 gggagaaacc atctcggctg ccgaggcact ggactccatc ggaaccgaag gcgtcgatag
      481 gctctccagc gcctggatgc caccggtgtc gccggccgtg ccgcgagcat tggtggccgc
      541 ccagttcgtg atgttcattg cggcggcgct ggccctcgcc gccgatggcc ggctgcccga
      601 aggcgaggta accgtctctg gagtcatggg cgccctcctg gcgatggtcg ggttggtctt
      661 ggccttcatc tcgtcgcgac ggctcggacc gtcgttgacg cccagcccgg tgccccggaa
      721 gaacgcccag atggtgatga gtggtcccta caggtatgcg cgtcatccga tctacggcgg
      781 gatctttctc gtgttgctcg gcactgccct tttcctggac agcgtcctag gggtggtagc
      841 cgccgtggtg gcgttcgtgt tcttctgggc gaagtcgacc ttcgaggagc ggcaactccg
      901 aatgaggttt gccggctaca agcggtaccg agaagtagtg caccgtcggc tgatcccatt
      961 cctgatctga cctctgacga gcgcgcgcct gctagttttc atgcatggtg cgagccagac
     1021 ctccgccaaa tcgctcctgt gagtgaccag acgacatcag gaggtaatga atggccaaga
     1081 ggtcgcgaga gcaccgccag gagatcccgg gatacgacga gagtcgaccc acgcgcgaag
     1141 agcgcaaact tcagcatcgg gcgacgagac atgccaccaa ccagatgctc cacacgatag
     1201 aggaccccga ggaggtcgtg cttcctcagg agcggcgaac caggaacaac gaaacgggca
     1261 acggcaacgc cgagcccgcc aagcgccgat tccgggtgtg gaagaccagg ttttggaagc
     1321 gccgggatga ctaccgcgac gagaaggcag ccctcgactc gaactggccg gtgatcacac
     1381 cgaaccagct gcaggaggaa tagacaccac cactaccctc gccgccgaga tggtgcgagg
     1441 acccgattga gattggtgat cgtcggcccg gggagggccg gccaatccat cgcgatcgca
     1501 tcggagcgct ccggccacga agtggttggt gttctcgccc ggactccagg gtcgacacct
     1561 tggcccgatc tcggctggga cataccactg cccgaaaccg acatcgtctt catcgcggtg
     1621 cgcgacgacg acatcgcgga ggtgtccgag caaatcgcac cgatcgtcga acacgcgggc
     1681 gtggtagccc atctatcggg gttcaccccc gttctcgccc ttcaccatct tcaggaaaga
     1741 gacatcgcgg ttggggggtt ccaccccctg gtgtcgctcc ccgatccgca gaccggtgcc
     1801 ggcgcactgg ccggcggctt tgtcggcata ggtggggatc cgttggcggt ggacatgctc
     1861 acccatttcg cctccaccct gggcatggag cacttccggc tcgatgatga agcccggcct
     1921 gcctatcacg ccgcggccgc tgccgcggcc aacttcgtcg tggcctctct ggcgttatcg
     1981 ggcgacttgt tcgactcggc gggcatcgac ccccgggtat cgaggccgct ggtggaaagg
     2041 gtcgttgcca acgtattcga caagggcccg gcggcatcgt tgaccggccc gatcgtgcgt
     2101 ggcgacatcg agacggtcat cggccatctg accgcggcac acgaggtctc cgactacgtg
     2161 ggtcgccagt tcaagctgat ggcggaagcg acggccattc gggccgggcg ggaggaggac
     2221 cttcgccgat ggaaatgacc cgcaccttcg acgagacacg aggacggtat gagggtcgag
     2281 tcggtttggt tcccaccctt ggctacttcc acgagggcca tttgaggctg atggacatcc
     2341 tccgggggca atgcgacacc ctggtggttt cgcttttcgt caatcccacc cagttcaacg
     2401 agaccgaaga cttcgaggcc tatccgagag acgagcaacg tgacggtgac ctcgccgccc
     2461 aacatggcgt cgatctcctc tttgcccccg cagtcgaaga ggtctatccg ggcccctcgg
     2521 tcaccaccgt ggaggtgatc ggggttaccg acgagatgga gggacgccat cgtcccggtc
     2581 acttcaaggg ggtcgccaca gtcgtcgcca agctgttcgc cggccttcaa ccggatgtcg
     2641 ccatgtttgg ccgaaaggac gctcagcagc tcgcggtgct gcaagcaatg gtgcgggatc
     2701 tgcgcttccc tatcgagatc gtggaagccc caatcatcag agagcccgat ggcctcgccc
     2761 tgtccagccg gaatgtgcgt ctcggcccta agtcccgcta caccgccctc gcaatctcac
     2821 gaggtctgtt ccaggcagcc gagcaggtcg atgccggaga acggcgtggc gatcgactgg
     2881 aggggacggt ggagcgctcg atgaagggtc tcgaagtcga ctacgtcaag ctggcctcac
     2941 aggagacgat gaagccgatc ggcaccctcg atcagcccgc agtcctggcc gcagccgctc
     3001 acgtcgacgg ggtgcggctc atcgacaacg tcgccttcga catggtggat ggtcgtcccg
     3061 ttcccgatcg aggcatcctc ctcgacgagc cgagcggctt gacggctctg ccatgatcct
     3121 tggctcacga tgagcgagaa cggcgatagc ggcgaacacg tcctggtcga gggaagacgc
     3181 caaaaacacg agcaggtgat cgctgccggt ggttatcccc atcgattcga gcgcacacac
     3241 ctggccgccg acctccagga gcgctattcc gacctcggcc cggagcagga gacggctgac
     3301 cgggtaaccg tggcgggccg tctcatgctg cacaggtcat tcgggaagct gcagttcggc
     3361 acgattcagg atgtcagtgg cgtcatccag ctgttcgtcg acaagcgagt cgcaggtgac
     3421 gcggccgacg aattcgccga gtacgacctt ggggattggg ttggtgcctc cggcgtgatc
     3481 atgaccacga aacgaggtga gctgagcgtg aaggtcgacg agctgacctt gctgcagaag
     3541 gctctgcgcc ctttgcccga caagtggcac gggctcgtcg acgtggagca gagaagtcga
     3601 cgcaggtacg tcgacctgat ggtgaacgac gaggcccgcc atgtggccac gaccagggcc
     3661 aagatcgtct cggagttgcg tcggcagttc gagtcgagag gattcatcga ggtggagaca
     3721 cccgtcatgc tcagccaggc caccggggct ctggcccgcc ccttcgtgac ccatcacaac
     3781 gcactcgacc tcgacctcca actccgcatc gcaaccgagc tctatctgaa gaggctcgtg
     3841 gtgggtgggc tggagaaggt cttcgagatc ggaaggatct tccgcaacga gggcatcgac
     3901 tccacccaca accccgagtt caccatgctc gaggcgtatc aggcccttgc cgactatcgg
     3961 gacatcatgg agctggtgga ggacgtcttc gtcgcagtcg tcgaggccgc cgtcgacacc
     4021 tcgcacccga cctacaaagg tcgagagctc tcgttcgaga agcccttccg gagggtctcc
     4081 ctcatcgaac tggtctccga gttcgtcggt gaggacgttt cactggacac agagagtcag
     4141 cggctctcgt cactgctcgc cgatcgggga gcgacggtct ccgccggagg tggcgtcggg
     4201 aagctcctct tcgagctcta cgagctggcc caccgcgaga tctgggaccc gaccttcgtg
     4261 atcgatttcc ccacggaggt ctcacccctg tcccggaaga gagacgacga cccgcggctg
     4321 accgagcgat tcgagttggt gatcgccggc gccgaatact gcaatgcctt ctccgagctc
     4381 aacgacgccg acgaccagct ggccaggttc gagacccagg ccgcggcgcg aaacgcaggc
     4441 gacgacgccg cccacgtaat cgatcgtgac tatgtggaag ctctggagta cgggttgccc
     4501 ccgaccggcg gcctgggcat cggcgtcgat cggctggtga tgctgctcac cgaccaagcc
     4561 catatccgcg aggtgatcct cttccccacg ctccgcccgg aggcatgaga accgttctat
     4621 gaaggtgcgg cggagtattt gcgcgttcag tccctcagga cggttgacgg cccgactgcc
     4681 cgggcgatct caacgaactg cgtcgacccg ctccagcagg tagacgccaa gactctcgag
     4741 gatggacttc ggcttcgatt tgggaagcgt ggcgagcgcc gactcggcga tggtgagtcg
     4801 ggtctttgcc gtctccaacg catggtcgat gtgaccccca cccttgacta ggtcgatgac
     4861 ctcggcgacg gtgtcgtcgc tgtaggggcg acctgtctcc aataggcccc gaacccggtc
     4921 cccatcgggg ccgttcactg cgcgcagcac ggggccggtg aaggtgccct ccctgatatc
     4981 ggagccggcc ggtttcccga tcgcgtcctc gtcggcgacc agatcgaggg cgtcgtcggc
     5041 gatctgaaag accatgccca cctcgaatgc ccaggtggac agggcgtcga ctgtcttctt
     5101 gtcggcctcg gccgccatgg cgcccaacct cgcagaagtg cggatcagag atgcggtctt
     5161 gccaccgatc acctggtggt aatcgtccag agaatgggtg agatcgtcgg tgagctgcag
     5221 ttcccgggtc tggccttcga ccaactcggc ataggtggcc gccagcaacc tcacggattc
     5281 ctggctgagg tgggttgcgg ccacctctga tgcccgggcc atgaggaagt ctcctgcgag
     5341 gatggcaacc gtgttggtcc agttggcatt ggccgaggtg gcaccacgac gcgtggcagc
     5401 ctcgtcgatg acgtcgtcgt gatagaggga gccgacatgg atcaactcga cggccaccgc
     5461 ggcctcgacc ggtcgcccgt cctcggagtc gccgaattcg gcggcgagga gagcgagaag
     5521 agggcggaac cgcttgcccc ctgccagcag gagatgctgg gcgatcttgg tcaaatatgg
     5581 gtcgtccgct gtcgaggcct cgagaagccg ctcctcgaca acattcagtt tctcccagat
     5641 tcggggaata ccaacaagac tttggaggtc ttccattggt gacgatgcta ttacgtcatg
     5701 ggaggcaccc aacctcgagg aaggcgaaga cgatgaccac aggtccactg ccccacgaga
     5761 tgagggtctg ttcccagccg atgctaaaat tgcatatgcc aggctcagga gtagcgaatg
     5821 tttgaacgct ttaccgaccg ggcccgaagg gttgtcgtac tggcccagga agaagcccgg
     5881 tttctaaacc acaactacat cgggaccgag cacatcctgt tgggtctcct caatgagggc
     5941 gagggaatcg ctgcgcaagc gttggagtct ctcgacatcg atcttgcctc cgttcgcgac
     6001 gaagtcgtca agatcatcgg ccagggacag cagtccccct ccggtcacat ccccttcacc
     6061 ccccgggcga aaaaggtctt ggagctctcc ttgcgggagg ctctccagct cgggcacaac
     6121 tacatcggca ccgagcacat cctgctgggt ctcatccgcg aaggcgaggg tgtcgccgca
     6181 caggtgctcc agcagctcgg ggcagagctc cagaaggtcc gccagaccgt catccagctt
     6241 ctctccggcc ccggtggctc cgaagagcag cagcccaagg cggcaggctc cggcggccgg
     6301 gagtcgagct cgtccgggtc caccgttctc gatcagttcg gacgcaacct cacccaatcg
     6361 gcccgcgaag gcaccctcga tcccgtcatc gggcgcgctc gcgagatcga acgtgtcatg
     6421 cagatcctct cccgccgcac caagaacaac ccggttctca tcggtgagcc cggagttggc
     6481 aaaaccgcca ttgtcgaggg tctcgctcag cgcatagtct ccgacgaggt cccggagaat
     6541 ctcaagggga agaacctcta cacactcgat ctcggggccc tggtcgccgg gtcccgttat
     6601 cgcggcgact tcgaggagcg tctcaagaag gtcctgaagg agatcaagag tcgaggtgac
     6661 atcgtcctgt tcatcgacga gatccacaca ctcgtcgggg cgggggctgc cgagggcgcc
     6721 atcgacgcag cttcgatact caagccgatg cttgcccgcg gtgagctgca gaccgtcggg
     6781 gccaccacac tcgaggagta ccgcaagtac ctggagaagg actccgctct ggagcgccgt
     6841 tttcagccga tccaggtcga agagccttcg ctcaccgaca ccatcgagat cctcaagggc
     6901 ttgaaggacc gttacgaggc ccatcactcg gtgcgtttca ccgatcaggc aatcgtgtcc
     6961 gccgccaacc tggccgaccg ctatctggcc gaccgcttct tgccggacaa ggccatcgac
     7021 ctcatcgacg aggccggctc ccgcatgcga atccatcgca agagcgacgt gcccgagatc
     7081 gtcgagatag acgacaagat cgccggagtc cgccgggaca agtcggaagc ggtgggaagt
     7141 gagaactacg agcgggccgc cagtctccgt gatgaggagc gctcgctgct cctggcgcgg
     7201 gccgagcacg agtccgggaa catggattcg ggctgggtga tcgacgagga agagatcgcc
     7261 gaggtcctgg cgcagtggac cgggatccct gtccatcgtc tcaccgaaga ggagaccgcc
     7321 cgtctcgtcc acatggagga cgagctccac aagcggatca tcggccagca cgacgccata
     7381 caggccgtgt ccaaggcgat ccgccgcacc cgggccgggc tcaaggaccc ccgccgtccc
     7441 tccggttcgt tcatcttcct gggtccgtcg ggtgtcggca agaccgagac cgccaaggct
     7501 ctgaccgagt tcttgttcgg tcaggaggac gccctcatcc aactcgacat gtcggagtac
     7561 atggagaagc acaccgtcag ccgtctggtc gg
//
LOCUS       DASUTA010000337         2648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_874934, whole genome shotgun sequence.
ACCESSION   DASUTA010000337 DASUTA010000000
VERSION     DASUTA010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874934"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VFZ80_06750"
     CDS             <1..837
                     /locus_tag="VFZ80_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6287167.1"
                     /translation="LGPNGAGKTTAIKVLLGLSRPTSGQATVFGLDAVRDSTAIRSRV
                     GYLAQDPRFYTHLTARETLEFTARFFFKGPRTAIDDRIEETLELVGLTDKADRPTKGF
                     SGGERQRLGIAQAQVNHPDLLVLDEPAAALDPLGRRDVLNIMERLREHTTIFYSTHIL
                     DDVQRVSDHVVILHNGRKVADATTSQLLDGGCTYQLDLRGNTARAKTTLEAEPWVSEI
                     VLSATESGDRMMVTVNDQKAGDALLLRSILGDNALTVTSFGRKQHNLEDVFLHLVEGD
                     ET"
     gene            834..1637
                     /locus_tag="VFZ80_06755"
     CDS             834..1637
                     /locus_tag="VFZ80_06755"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6287168.1"
                     /translation="MTATTRVEAIDLLPAGGSGWTVGLNNLLRKEFGQWWTTKLWWIQ
                     TLIWLIILNGVTTVIMLDSSGMAPTDLVNEAVQTFFLVAATAVTIGIVLTLQGSIVGE
                     RELGTAAWVVSKPVSRTAFVASKLIAHFTGFAITAILVPSAVFLVTARLLLPEPVAYG
                     PFAMGMAVTALNVLFYVVLTLALGCMFKGRGPIAGIGITLILMGQFFKGMLPEAIVLA
                     TPWLLGDVAASFAIQQPPAFDRTVPMIVAGIEIVILAIVGIWRFNREEF"
     gene            1691..>2648
                     /locus_tag="VFZ80_06760"
     CDS             1691..>2648
                     /locus_tag="VFZ80_06760"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX6287169.1"
                     /translation="MLDLIRNLAILFAFAWLARSLLGTREITWRRTLLATLLGLLSGA
                     IGAAALLVSDLDTFDAFDTIRTQFYLLMLLFAVPATMALMVFFELLLATRDVHRRFRP
                     FRTLKRWFRMWVRGFQVTRIATRHGVAPLLGLGRGESSARTPAEIARRTRLSLEEAGG
                     MFVKLGQLLVTRPDLLPPEAVRELSLLHADVPPIPDEHVKAIVHEDTGRPVEEVFSEV
                     TWEPLGSASIGQAHAARLLDGTEVVVKVRRPGLEEQVDTDLAIVRWLADVAKRRTGWG
                     KAYDVTNLAAEFATALRAELRFSVEARYAVEIGAACAGDPLIR"
BASE COUNT          479 a          845 c          831 g          493 t
ORIGIN      
        1 ctggggccaa acggcgcagg caagaccacc gcgatcaagg tgttgctggg cctcagccgt
       61 cccacctccg ggcaggcaac tgtgttcggt ctcgatgccg tgcgtgacag cactgcaatc
      121 aggtcacgag ttggctacct cgcccaggat ccgcgcttct acacccacct gacggcgcgg
      181 gagaccttgg agttcaccgc acgcttcttc ttcaaaggac ctaggacggc aatcgatgac
      241 cgaatcgaag agactctcga gctggttggt ctcaccgaca aggccgaccg accgaccaaa
      301 gggttctctg ggggcgagcg acagaggctg ggaatcgccc aggcccaggt caaccatccc
      361 gacctgctcg tgctcgacga accggccgcg gcactcgacc cactggggag acgcgacgtg
      421 ctcaacatca tggagcgtct gcgcgagcac accaccatct tctattcgac tcacatactc
      481 gacgacgttc agcgggtcag cgaccacgtg gtgatcctcc acaacggacg gaaagtcgca
      541 gacgccacca cgtcacagct cctcgatggc ggatgcacct accagctcga ccttcgtggt
      601 aacaccgcca gggccaagac cacgctcgag gcagagccct gggtgagcga aatcgtcctg
      661 tcggcgacgg agagcggaga ccggatgatg gtcaccgtca acgaccagaa ggccggcgac
      721 gccttgctcc tgcggtcgat cctcggcgac aacgccctga ccgtgaccag cttcggccga
      781 aagcaacaca atctggagga cgtcttcctc catctggtgg aaggagacga gacatgacgg
      841 cgacaacacg ggtcgaggcc atcgatctac tgccggccgg cgggagtggc tggacagtcg
      901 ggctcaacaa cctcctgcgc aaggagttcg gtcagtggtg gacgacgaag ctgtggtgga
      961 tccagaccct gatctggctg atcatcctca acggggtcac caccgtgatc atgctcgaca
     1021 gttcgggaat ggccccgacc gacctggtca acgaagcggt gcaaacgttc ttcctggtcg
     1081 cggcgaccgc cgtgacgatc gggatcgttc tcaccctcca ggggtccatc gtcggcgagc
     1141 gggagctggg aaccgcggca tgggtggtgt ccaagccggt gtcacgcacc gccttcgtgg
     1201 ccagcaagct catcgctcat ttcacgggct ttgccatcac cgccatcctc gtcccgtctg
     1261 ccgtgttcct cgtgacagcg cggctcctcc tgcccgagcc cgtggcttac ggccccttcg
     1321 cgatgggcat ggcggtcaca gcgctcaatg tgttgttcta cgtcgtactc acgctggcgc
     1381 ttggctgcat gttcaagggg agagggccca tcgccggcat cggcatcacc ttgatcctga
     1441 tggggcagtt cttcaagggg atgctcccgg aggcgatcgt gctcgccacc ccatggttgc
     1501 tcggggacgt ggccgcgtca ttcgcgatcc agcagccgcc ggccttcgac cgcacggtgc
     1561 cgatgatcgt cgccgggata gagatcgtca tcctggcaat cgtgggcatc tggcgattca
     1621 accgtgagga attctgacaa caccgaatcg gcgaccggcg cgaccagtcg cagaaaggtg
     1681 agcgagccgg atgcttgacc tgattcgcaa cctcgccatc ctgttcgcct tcgcctggct
     1741 ggcccgatcg cttctcggta cacgtgagat cacgtggcga aggactctcc tggccacgct
     1801 cctggggctg ctctcggggg ccattggcgc ggcggccctt ctggtaagcg acctcgacac
     1861 gttcgacgcg ttcgacacga tcaggaccca gttctacctc ctgatgctcc tcttcgccgt
     1921 gccggcgacg atggcgctca tggtgttctt cgagctgctg ctggcgaccc gggacgtcca
     1981 tcggcgattc cgaccgtttc gcacgttgaa gagatggttc cggatgtggg tcagaggttt
     2041 tcaggtaacc cggatcgcga ctcggcatgg agtcgctccc ctgctcggtc tgggcagggg
     2101 agagtcatct gctcggactc cggccgagat tgcacgacgg acacgtttgt cgctcgagga
     2161 ggcaggcggg atgttcgtca agctgggtca gcttctcgtg acccgacccg acctcttgcc
     2221 gcccgaagca gtgagagagt tgagcctgct ccacgccgat gtaccgccca tacctgacga
     2281 gcatgtgaag gcgatcgtcc atgaggacac gggaaggcca gtcgaggagg tcttctccga
     2341 ggtcacatgg gagccgctgg gctcggcctc gatcggtcag gctcacgctg cccgactcct
     2401 cgatggcacc gaggtggtgg tcaaggtacg ccgtcccggt ttggaagagc aggtggacac
     2461 cgacctggcg atagtccgct ggctggccga tgtcgccaaa cgccgaaccg gctggggtaa
     2521 ggcgtacgac gtcaccaacc tggctgcaga gttcgcaacc gctctacgtg ccgagcttcg
     2581 tttctcagtt gaggctcggt atgccgtcga gattggagca gcctgtgccg gcgatcctct
     2641 gattcgtg
//
LOCUS       DASUTA010000338         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_747471, whole genome shotgun sequence.
ACCESSION   DASUTA010000338 DASUTA010000000
VERSION     DASUTA010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747471"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFZ80_06765"
     CDS             <1..288
                     /locus_tag="VFZ80_06765"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEX6287170.1"
                     /translation="STKNPAYSDLLYVESLVARDTVNTMPLDTIDAYQDHGDPTPASF
                     DAVDIVRANDLLDKMAEVGIDYEDVVETLEREGVEKFTASWLQLLDEVEKA"
     gene            289..996
                     /locus_tag="VFZ80_06770"
     CDS             289..996
                     /locus_tag="VFZ80_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287171.1"
                     /translation="MSHAREDDQLILGASDRIELIATIVMALAAIATAWCAFQATKWS
                     GIMAIEFSNANAGRVESTRVDSLANTQRSIDVGVFTSWLDAVAAEVDAGEIPPVRESG
                     YVPVEGTLSAFYYERMRDEFRPALDAWLAADPLNDPEAPATPFAMEEYELAAATEADE
                     LLEQSAQDRDDALDANQNGDNYVLTTVGFALVIFFAGVSSKLKETRNRWIAISVALVL
                     FAGGLIAVFSLPIVTPF"
     gene            complement(1116..>2092)
                     /locus_tag="VFZ80_06775"
     CDS             complement(1116..>2092)
                     /locus_tag="VFZ80_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX6287172.1"
                     /translation="KRVINEPRRGIGSTTVAHVDRYTQTKGITFFEGLRRASDIPQLN
                     SRAVKSVQEFVALIDMLSEKAEEGGVQAAVQAVLDDTGMVATYESERTIEAMGRVENL
                     RELVGVAAEFEASNEGAVIGDEEFDDLEDLRRLEIFLESTALVADIDEWEEGAGAVTL
                     MTLHTAKGLEFPAVFIVGMEDGVFPHMRSLGEPAELEEERRLAYVGITRAQDKLYLTS
                     AWQRMLFGGSSYNPPSRFLQEIPDALMSKAPKRQKQRPADRAAASGPRTTVSAGQVAP
                     GDRVRHDKWGLGTVREIVGEGDRAEAEVMFDTQGKKRLLLAWAPLEKA"
BASE COUNT          364 a          696 c          640 g          392 t
ORIGIN      
        1 tcgacgaaga acccggccta cagcgacctc ctctatgtgg agagcctggt ggccagggac
       61 acggtcaaca cgatgccact cgacaccatc gacgcctatc aggatcacgg cgaccccact
      121 cccgccagct tcgacgccgt cgacatcgtc cgggccaacg acctgctgga caagatggcc
      181 gaagtgggca tcgactacga ggatgtcgtc gagactctcg agcgcgaggg ggtcgagaag
      241 ttcacggcga gctggctcca gcttctggat gaggtggaga aggcgtagat gtcccatgcc
      301 agggaggatg accaactcat cctcggggcc tcagatcgaa tcgagctgat cgccacgatc
      361 gtgatggccc tggccgcgat cgccactgcc tggtgcgcct ttcaggccac gaagtggagc
      421 ggcatcatgg ccatcgagtt cagcaatgcc aacgcgggca gggtcgagtc cactcgtgtc
      481 gactcgctgg cgaacacaca acgatcgatc gatgtcggcg tgttcacttc atggctcgat
      541 gccgtggccg cggaggtcga tgccggggag atcccgccgg tgcgggagag cggttacgtg
      601 cccgtcgagg gcaccctgtc ggccttctac tacgagagaa tgcgggatga gttcaggccg
      661 gccctcgacg cctggctggc ggccgacccg ctcaacgatc ccgaggcacc ggcgactccg
      721 tttgccatgg aggagtatga gttggcagcg gcaacggagg ccgacgagct cctggagcag
      781 tccgcccaag accgtgacga tgccctggat gccaatcaga acggcgacaa ctacgtgttg
      841 accaccgtgg gcttcgccct ggtcatcttc ttcgcaggtg tcagctccaa gttgaaggag
      901 acacgcaacc ggtggattgc gataagtgtc gccctcgtcc tgtttgcggg gggcctgatt
      961 gcggtgttca gccttccgat agtgacgccg ttctagggcg gtcgcccgtc agtcccagtc
     1021 gtcccagctc agccccgcgc tctcgttacc cgggcccctg actccggtca gcgaagtctg
     1081 caaccccggg ttgaaccgca ccgtgtcgaa ggggatcacg ccttctccag cggggcccag
     1141 gccagcagca accgcttctt cccttgggtg tcgaacatga cttcggcttc ggcccgatcg
     1201 ccctcgccga cgatctccct cacggtgccc aggccccact tgtcgtgtct gacccggtcc
     1261 ccgggcgcca cttgaccggc ggacaccgtt gtcctcggcc ctgaagccgc agcacggtcg
     1321 gcgggcctct gcttctgtcg cttcggggcc ttgctcatca gcgcatcggg aatctcctgg
     1381 agaaaacgtg acggcgggtt gtagctcgac ccaccgaaca acatcctttg ccaggcggat
     1441 gtcagataga gcttgtcctg ggcacgggtg atgcccacgt aggcgaggcg acgctcctct
     1501 tcgagctctg cgggctcacc gagagaccgc atgtgaggga acacaccatc ctccatgccg
     1561 acgatgaaca cggcagggaa ctcgaggccc ttggccgtgt gcagagtcat gagagtcacc
     1621 gctccggcgc cctcttccca ctcgtcgatg tcggcgacga gtgccgtcga ctccaggaag
     1681 atctcgagac ggcggaggtc ctccagatcg tcgaattcct catcgccgat caccgcgccc
     1741 tcatttgatg cctcgaactc ggctgccacg ccgacgagct cacgcaggtt ctcgacccgt
     1801 cccatcgcct cgatggttcg ctccgactcg taggtggcga ccatcccggt gtcgtcgagc
     1861 acggcctgca ccgccgcctg aacaccgccc tcctccgcct tctcgctcag catgtcgatg
     1921 agggcaacga actcctggac cgatttgacg gctcgcgaat tgagttgcgg gatgtcgctc
     1981 gcccgccgca gcccttcgaa gaaggtgatt cccttcgtct gggtgtatcg gtcgacgtgg
     2041 gcgacagtgg tactcccgat ccctcttctc ggctcgttga tcaccctctt ga
//
LOCUS       DASUTA010000339         3088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_100765, whole genome shotgun sequence.
ACCESSION   DASUTA010000339 DASUTA010000000
VERSION     DASUTA010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100765"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..358)
                     /locus_tag="VFZ80_06780"
     CDS             complement(50..358)
                     /locus_tag="VFZ80_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX6287173.1"
                     /translation="MGQLALIVTSTAKDGRRDEIRELYEEIMAPRATENDSQEVVVWC
                     ADQHDPNTFYLFEIYSDPAAMGANAQAGWFGEYMARVGPLLAGEPKVAMATPMWTKGV
                     "
     gene            451..804
                     /locus_tag="VFZ80_06785"
     CDS             451..804
                     /locus_tag="VFZ80_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287174.1"
                     /translation="MANAKYSGPPEKLELYEELVATVPGLDRKGAANPYTSRSGWMQS
                     FLDKEGSISIRLGKEDLETFLERYDTELSVQYGSVMKDFAVVPDRLLGTDELSDWFAR
                     SWEWVGTLKPKPTTK"
     gene            complement(937..1362)
                     /locus_tag="VFZ80_06790"
     CDS             complement(937..1362)
                     /locus_tag="VFZ80_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6287175.1"
                     /translation="MEQRLSLVTLGVADLELSRTFYRNLGWKEGPGSSDEVVFFQVNG
                     MVVGLWDRDSLAEDSTVDDDGGWGGITLAYNTRSQSQVDAVLEEAQFAGATIGRPGAE
                     TFWGGYSGVFIDPDGHPWEVAYNPGWSIDDDGNVSLGEG"
     gene            complement(1403..1888)
                     /locus_tag="VFZ80_06795"
     CDS             complement(1403..1888)
                     /locus_tag="VFZ80_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287176.1"
                     /translation="MKSALAGLALIVSVASCTASGAESTDPPELTDAYGCGHGFYLGA
                     PDQTAGLFIISQSGFGEEVPTGAHQLPSDAWVSELQFGTDLFANWCDDVLEPGEPEPV
                     TEEIWQVSGRLEITSLPAPGECGPASGFLSGTVARNDVGDEIPLGDIDLENGSWGCFA
                     G"
     gene            1980..>3088
                     /locus_tag="VFZ80_06800"
     CDS             1980..>3088
                     /locus_tag="VFZ80_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX6287177.1"
                     /translation="MTRNIPDKPSLEGLEAKWDQHWEDEGTYRFDRTKTRDEIFAIDT
                     PPPTVSGSLHMGSVFGYVQTDTHARYRRMSGKEVFYPMGWDDNGLPTERRVQNYYGVR
                     CDPTLPYDQAFTPPEQPHDPPISISRRNFIELCHVLTNEDEKIFEDIWRSLGLSVDWT
                     QTYATIDVHSQRTSQRAFLANLERGEAYSSDAPVLWDVDFQTAVAQAELEEREKGGAY
                     HRVAFHKTDGERIYIETTRPELICSCVALVANPEDERYQPLFGSTVTTPLFGVEIPVV
                     AHELADPEKGTGVAMICTFGDTTDVTWWRELDLPTRAVIDHDGRFQEETPEWLTGAAR
                     EHYASLAGLFANQARHKMAEMLAESGDLDGEPRQI"
BASE COUNT          581 a          981 c          975 g          551 t
ORIGIN      
        1 ccgagctcga gtcacgtgcc gaggtcgccg cttgctggta cggcggtctc tagacgccct
       61 tcgtccacat cggggtggcc atggccacct tgggctctcc ggccaggagc ggcccgaccc
      121 gcgccatgta ctcgccgaac cagcccgctt gcgcgttggc gcccatggcg gcggggtcgg
      181 agtagatctc gaacaagtag aaggtgttcg ggtcgtgttg gtcggcgcac cagaccacca
      241 cttcctgaga gtcgttttcg gtggcgcgcg gcgccatgat ttcctcgtac aactccctga
      301 tctcgtccct cctgccgtct tttgccgtcg acgtgacgat gagtgccagt tgtcccatct
      361 catgatcctt ctgtcgctga ggcgaacgta ggctcgctgt ggcaggccgg ccatccatcg
      421 aaaggtgggt ctgcgtaggg tcggggggtc gtggccaatg ccaagtactc cgggcctccc
      481 gagaagctgg agctgtatga ggagctcgta gcgacggtgc cggggctcga tcgcaagggc
      541 gccgccaacc cgtacacctc acgcagcggt tggatgcagt ccttcctcga caaagagggg
      601 tcgatatcga tcaggttggg caaggaagac ctggagacgt tcctcgaaag gtacgacacc
      661 gagctctctg tccaatacgg gtcggtgatg aaggatttcg ccgtggtgcc cgaccggctt
      721 ctcggcaccg acgagttgag cgactggttc gcacgcagct gggagtgggt ggggaccctg
      781 aagccgaagc cgaccacgaa atagccacaa ggcgctcacc aacggtgacc ctggccaaac
      841 ctccggatgg gcgtgcacga cgttgcggaa aacgcaacgc tgtgcacacc caggcgaagt
      901 tcacccgggc gtcggggccg cagcccgggg tcggaatcag ccctcaccca ggctgacgtt
      961 tccgtcgtcg tcgatgctcc agccggggtt gtaggccacc tcccaggggt ggccgtcggg
     1021 gtcgatgaac accccggaat atccgcccca gaaggtctcc gccccggggc gaccgatggt
     1081 ggctccggcg aactgggcct cctcgagcac cgcatcgacc tggctctggc tccgggtgtt
     1141 gtaggcgagt gtgatgccac cccagccccc gtcgtcgtcg acggtgctgt cttcggccag
     1201 ggagtcgcgg tcccagaggc cgacgaccat cccgttgacc tggaagaaca ccacttcatc
     1261 ggaggaaccg gggccttcct tccatccgag gttgcggtag aaggttcggg agagctccag
     1321 gtcggccaca cccagggtca cgagactgag acgttgttcc atacccggtc aggctaggca
     1381 gacgtggtga caggaactcg actcagccgg cgaagcagcc ccatgagccg ttctcgaggt
     1441 cgatgtcgcc gagcggtatc tcgtcgccga catcgtttcg tgcgaccgta ccggacagga
     1501 acccggatgc aggcccacac tcgcctgggg ccggcaagga cgtgatctcg agtctccccg
     1561 agacctgcca tatctcctcg gtgacgggtt ctggctcgcc cggctcgagg acatcgtcgc
     1621 accagttggc gaacaggtcc gtgccgaact gcagctcaga gacccaggca tccgacggca
     1681 gctgatgagc ccctgtcgga acctcctccc cgaacccact ctgggagatg atgaagagac
     1741 cggcggtctg gtcaggtgcc ccgaggtaga acccgtgccc gcagccgtat gcatctgtca
     1801 actcgggcgg gtcggtgctc tcggcaccgc tcgcggtgca tgatgcgacc gagacgatca
     1861 gggccaggcc ggcaagtgcg ctcttcatgg agattcgacg tgagcggtga atcggcagtt
     1921 cccggccaat ccaccgactc cctatccgaa agcgtcaacc gttaccctcg gtcgggacga
     1981 tgacgagaaa catccctgac aagccgagtc tcgagggtct cgaggcgaaa tgggaccagc
     2041 actgggagga cgagggcacc taccgattcg acaggacaaa gactcgtgac gagatcttcg
     2101 ccatagacac cccaccgcca accgtgtcgg gctcattgca catggggtcg gtgttcggct
     2161 atgtgcagac cgacactcac gcccgctatc gccgcatgtc gggcaaggaa gtgttctatc
     2221 ccatgggttg ggacgacaac gggcttccga ccgagcgccg cgtgcagaac tattacgggg
     2281 ttcggtgtga cccgacgttg ccttatgacc aggccttcac accacccgaa cagccgcacg
     2341 acccacccat ctcgatcagc cggcgcaatt tcatcgagtt gtgtcatgtc ctcacaaatg
     2401 aggacgagaa gatcttcgag gacatctggc gcagtctcgg gttgtccgtg gattggaccc
     2461 agacctacgc caccatcgac gtccacagcc agaggacgtc acagcgcgcc ttcctcgcca
     2521 atctggaaag aggagaggcc tactcgtcgg atgcgccggt cctctgggac gtggactttc
     2581 agacggctgt cgcacaggca gaactcgagg agagggagaa aggtggcgcc tatcaccgtg
     2641 tcgccttcca caagaccgat ggcgagcgga tctacatcga gacgaccaga ccagagctga
     2701 tctgttcctg tgtcgccctg gtcgccaacc ccgaagacga gcgatatcag ccactcttcg
     2761 gctcgacggt gacaactccg ctcttcgggg tcgagatccc tgtcgtcgct cacgagctgg
     2821 ctgatccgga gaagggcacg ggtgttgcca tgatctgcac cttcggtgac accaccgacg
     2881 tcacatggtg gcgtgagctc gatcttccga ctcgggccgt catcgatcac gacggccggt
     2941 tccaagagga gactccggag tggctgaccg gtgctgctcg cgagcactac gcctcgctgg
     3001 cggggctctt cgccaaccag gcacgtcaca agatggccga gatgctggcc gagtcgggcg
     3061 atctcgatgg tgagcctcga cagatcat
//
LOCUS       DASUTA010000340         4403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1914977, whole genome shotgun sequence.
ACCESSION   DASUTA010000340 DASUTA010000000
VERSION     DASUTA010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1914977"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VFZ80_06805"
     CDS             complement(<1..312)
                     /locus_tag="VFZ80_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEX6287178.1"
                     /translation="MAFPDGLADFRSDTVTRPTPAMLEAMASAPLGDDVYHDDPTVNL
                     LEEESAASVGKEGAVFVPTGTMGNQLSIMFHTNPGEEVLANAASHLRSIEAGAAQAFS
                     GV"
     gene            complement(461..892)
                     /locus_tag="VFZ80_06810"
     CDS             complement(461..892)
                     /locus_tag="VFZ80_06810"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEX6287179.1"
                     /translation="MTNEETSRVRPDEVLPPDGKSIQSRELVKDAVFMMPNIVKLVGR
                     LLRDPRVPRRAKITLGLAAAYIMSPIDLIPEVIPVVGWADDVIIMMFAIDSLIDRAGP
                     EIVEEHWDGPGDLLGLVRDVVAISRNIVPKRLGQIIDRLSG"
     gene            complement(889..1059)
                     /locus_tag="VFZ80_06815"
     CDS             complement(889..1059)
                     /locus_tag="VFZ80_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287180.1"
                     /translation="MAALSRAGRRASIHLVQALIEGLKAVEAVIEEIGGIADQSDHDD
                     SDNDHLERIEIE"
     gene            complement(1077..2255)
                     /locus_tag="VFZ80_06820"
     CDS             complement(1077..2255)
                     /locus_tag="VFZ80_06820"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEX6287181.1"
                     /translation="MRIVLVTGKGGVGKTTVAAATALAAADSGYRTLVTSTDPAHSLA
                     DALEVHLESDPTQIVERLDGQQIDTQKQLDRYWGSIRDQLMDVLDWGGAAGIEAEEFL
                     VFPGMDELFALLEVNRHARSGEYDVLVVDCAPTAETLRLLSLPEVLSWYFEKVLPTER
                     KLMKAARPLLNRITNLPLPEDEVFTAAQSVFESVDGVKELMSKPEITSARLVVNPEKM
                     VIDEARRTYSYLGLFGYGVDGVVVNRVLPDLVADPYFERWRSIQKDHLDNIDDAFAEV
                     PRLRLRLFDDEMVGVDRLRVMASELYQDRDAIADFSATTPFRVVEDGATVTMELDVPF
                     FDKSDLDVFRHGHELYVQLGSYRRSFLLPDALQRRQVSRARLDAGTLRVSFVKPEKSK
                     "
     gene            complement(2257..2697)
                     /locus_tag="VFZ80_06825"
     CDS             complement(2257..2697)
                     /locus_tag="VFZ80_06825"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6287182.1"
                     /translation="MTEGTFSTLEIDAPRETLYDVAADVAAYPDWASGVKEVEILALD
                     PEGRVDRARFVLEGFVKEIEYVLKYTHDRPDNLSWVAEESDDLKMLEGSYQFTSSDSG
                     ATEVVYSLTVEPNFIIPGFIRRQAEKQIVSTALRGLRKRVSSGH"
     gene            complement(2743..3528)
                     /locus_tag="VFZ80_06830"
     CDS             complement(2743..3528)
                     /locus_tag="VFZ80_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6287183.1"
                     /translation="MIFVSDVHDSPPALRRLTALGEEIVILGDLVNLTDYRTGEGAVA
                     AVMGTEFASRSSAARGKGAYSEMRSLWEERSAGSAEELRQLIGAELTEQYRNAGEALT
                     GGHGLVIHGNVDRPGPLQEILPDGFRYVHGEVVVRDGMRLGFVGGGVPTPLQAAGEVS
                     DEDMKAILHSIGPVDVLCTHVAPALTPLRRDVVTGREERGSDQVYEYLLENQPRYHLF
                     GDVHQPQASTWRIGRTRCFNAGYFRATGRYLRLDDGLVHLGLV"
     gene            complement(3525..3821)
                     /locus_tag="VFZ80_06835"
     CDS             complement(3525..3821)
                     /locus_tag="VFZ80_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287184.1"
                     /translation="MVKVYAVVLVLGIVGLLIYILGGAFAENLGREAKGPDARFGLGG
                     KLLVAAAVGFGMGGMSAEFSPLDLSWPIALVIAVVAAGLSMLWVRYAVGQSGTR"
     gene            complement(3885..4073)
                     /locus_tag="VFZ80_06840"
     CDS             complement(3885..4073)
                     /locus_tag="VFZ80_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287185.1"
                     /translation="MLIDCEQCAMRDTSACDDCVVTCLLGGGPVDLSDEQAEAVANLA
                     EAGLVPKLRLVPAERRVS"
     gene            4202..>4403
                     /locus_tag="VFZ80_06845"
     CDS             4202..>4403
                     /locus_tag="VFZ80_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEX6287186.1"
                     /translation="MLIVNAFFVAGEFALVTVERSKVARRAGEGDRRAKRIHNGLSRL
                     SFHLSGAQLGITVSSLILGLVAE"
BASE COUNT          809 a         1381 c         1378 g          835 t
ORIGIN      
        1 cactcccgag aatgcctgag cagcgccggc ctctatggag cggaggtggc tggcggcgtt
       61 ggccagcacc tcctcgcccg ggttggtgtg gaacatgatg gagagctggt tacccatggt
      121 gccggtcggc acgaaaaccg ctccctcctt gcccaccgag gctgcggact cctcctcgag
      181 aaggttcacc gttgggtcgt cgtgatagac gtcgtcaccc agcggggccg atgccatcgc
      241 ctcgagcatg gccggcgtgg gccgggtcac ggtgtcggat ctgaagtcgg ccagaccgtc
      301 gggaaaagcc atgaggggat ggtaggccct accgcgacct ccggtagttg ggctcatatc
      361 cggctcttac tgcctccaga gactctggag gcagtaagag cagtggctct cgaggtagtt
      421 agagccatat atggctctaa ctaccggagg tcgagttggg tcagccgctt aggcggtcga
      481 ttatctgacc gagccgtttc gggacgatgt tgcgtgatat ggcgactacg tcgcggacca
      541 ggccgaggag gtccccgggc ccatcccaat gctcctcgac gatctccggc ccggcgcgat
      601 cgatgagcga gtcgatggca aacatcatga tgatcacatc atcggcccag ccgactacgg
      661 ggatgacctc ggggatgaga tcgatcgggc tcatgatgta ggcagcggcg agtccaaggg
      721 tgatcttggc ccgtctcgga accctgggat ccctgaggag ccgtcccacc agcttcacga
      781 tgttgggcat catgaagacg gcatccttga cgagctctcg cgactggatg ctcttgccgt
      841 ccggcggcag cacttcgtcg ggtcgcaccc gactcgtctc ttcgttggtc actcgatctc
      901 gattctctca agatggtcgt tgtcggagtc atcatggtct gactgatcgg caatgccacc
      961 gatctcttcg atgaccgcct ccacggcctt gagaccttcg atgagagcct gtacgagatg
     1021 aatcgaggcg cggcgtccgg cgcggctcag ggcagccatc gttggagatt ggtccctcac
     1081 ttcgatttct ccggtttgac gaacgagact cgcagtgtcc cagcgtccag acgcgcccga
     1141 ctgacctgac gtcgctgcaa cgcatcggga agcaggaacg accgacgata cgagcccagt
     1201 tggacataga gctcgtgtcc atggcgaaag acatcgagat ctgacttgtc gaagaagggc
     1261 acgtctagct ccatggtgac cgtcgcacca tcttcgacga cgcgaaaggg cgtggttgcg
     1321 gagaagtcgg cgatggcatc ccggtcctgg tagagctccg aggccatcac ccgaagccgg
     1381 tcgacaccca ccatctcgtc gtcgaagagc cggagccgca accgggggac ctcggcgaag
     1441 gcgtcgtcga tgttgtccag gtggtccttt tggatggagc gccacctctc gaagtagggg
     1501 tcagcaacca gatcgggcag gaccctgttg accacgacgc catcgacacc gtatccgaac
     1561 agccctagat agctgtaggt gcgtctcgcc tcgtcgatga ccatcttctc ggggttgacc
     1621 acgaggcgag ccgaggtgat ttctggcttc gacatcagct ctttgacccc gtcgacggac
     1681 tcgaacaccg actgagcagc tgtgaacacc tcgtcctcgg gcagagggag gttggtgatc
     1741 cggttgagca agggccgtgc agccttcatc aacttacgct cggtggggag caccttctcg
     1801 aagtaccaag agagcacctc gggcagggag agaagccgca gggtctccgc agtgggtgcg
     1861 cagtcgacta ccaggacgtc gtattcaccc gatcgagcgt gcctgttcac ctcgaggagg
     1921 gcgaacagct cgtccatccc ggggaacacc aggaactcct cggcctcgat gccagcagcg
     1981 cccccccaat cgagcacatc catcaactgg tccctgatcg acccccagta tcgatccagt
     2041 tgtttctggg tgtcgatctg ctggccgtcg aggcgctcga cgatctgggt cggatcactt
     2101 tcgagatgga cttccaaggc gtccgccagg gaatgggccg ggtccgtgga cgtgacgagg
     2161 gtcctgtagc cggagtcggc ggcggccaat gccgtggcgg cggcgactgt tgtcttgccg
     2221 actcccccct tgccggtgac caggacgatt cgcatgtcag tggccgctgg aaactcgttt
     2281 tcgcagtccg cgcagtgccg tggacacgat ctgtttctcc gcctgacgcc ggatgaaccc
     2341 gggaatgatg aagttgggct cgaccgtgag gctgtacacg acctcggttg cgccgctgtc
     2401 ggaactggtg aactgatatg agccctcgag catcttcaag tcgtcgctct cctcggcgac
     2461 ccagctcagg ttgtcaggtc ggtcgtgggt gtacttcaag acgtactcga tctctttgac
     2521 gaagccttcc aggacgaatc gggctcgatc gacgcggccc tccggatcga gggccaggat
     2581 ctcgacctcc ttcaccccac tcgcccaatc cggatatgcg gcgacgtcgg cggccacgtc
     2641 gtagagagtc tctcggggcg cgtcgatctc cagggtggag aaggttcctt cagtcatctg
     2701 aatgcctgaa tctagtgcgg cgccgcgctt ggcgatcctc cactagacca acccgaggtg
     2761 gacgaggccg tcgtcgagtc tcagatatcg tccggtcgcc cggaagtagc cggcgttgaa
     2821 gcacctcgtg cgtcctattc gccaggtgga cgcttggggt tggtggacat caccgaacaa
     2881 gtgatagcgg ggctggttct ccaggaggta ttcgtacacc tggtccgagc ctcgctcctc
     2941 tcgccctgtc acgacgtccc gtcgcaacgg tgtgagggcg ggagcgacgt gggtacagag
     3001 aacatcgacc gggccaatgg agtggaggat cgctttcata tcctcatcgg acacctcgcc
     3061 cgcggcttga aggggggtgg ggacgccccc gccgacgaag cccagccgca tcccatccct
     3121 gacgacgacc tcgccgtgca catatcggaa accgtccggg aggatctctt gcagcgggcc
     3181 gggccggtcg acgttgccat gaatgaccag gccatggccg cctgtcaggg cctcgccggc
     3241 gttccggtat tgctcggtca gctctgcgcc gatcagttgg cggagctcct cggccgaccc
     3301 ggcggagcgt tcctcccaga gagagcgcat ctccgagtac gccccttttc cccgtgcggc
     3361 ggacgagcgc gaggcgaact cggtccccat gaccgccgcg accgcgccct cgccggtgcg
     3421 atagtcggtg agattcacca gatcgcccag gatgacgatc tcctcgccga gggctgtcag
     3481 cctccgcagt gccggaggtg agtcgtgaac atcggagacg aagatcatcg agtcccgctc
     3541 tgacccacgg catagcgaac ccagagcatc gacaggccgg cagcgaccac cgctatgacc
     3601 agggcaatcg gccaggagag atcgagtgga gagaactcgg ccgacatgcc acccatgccg
     3661 aacccaaccg cggcagcgac gagcagtttc ccgccgagcc cgaaacgggc gtcggggcct
     3721 ttcgcctctc tccccagatt ctcagcgaag gcgccaccga ggatgtagat cagaagcccg
     3781 acgatgccaa gaacgagcac tactgcatag accttgacca cggttcggca gggtaaccgt
     3841 ggtctcgctg gtctagcgag acccgatcat catgcggtct cgggtcacga gacacgtctc
     3901 tctgcaggca ccaaacgcag cttcgggacc agaccggcct cggccagatt ggccaccgcc
     3961 tcggcctgct cgtcactcaa gtcgacgggg cctcctccga gcagacaggt gacgacgcag
     4021 tcatcgcaag cactcgtgtc ccgcatcgcg cattgttcgc aatcgatcag catctcgacc
     4081 tcctggtggc caagttacgg ggcgaccggg acagattctg cggggttagt ctgttcccat
     4141 tccgtgagca tcgaggttcc gtcctattag tacgaccgcc ggtctggctc tggtcgcgtt
     4201 cctgctcatc gtcaacgcct tcttcgtcgc gggcgagttc gccctcgtca cggtggagcg
     4261 gagcaaggtc gcccggagag ctggagaagg cgatcgcagg gcgaagcgga tacacaacgg
     4321 attgtccaga ctctcttttc atctctccgg ggcccagctc ggtataaccg tctcctctct
     4381 gatcctcggt ctcgtggcag agc
//
LOCUS       DASUTA010000341         2458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1266772, whole genome shotgun sequence.
ACCESSION   DASUTA010000341 DASUTA010000000
VERSION     DASUTA010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266772"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VFZ80_06850"
     CDS             complement(<1..461)
                     /locus_tag="VFZ80_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX6287187.1"
                     /translation="MLQPRSVAVVGASERRGSVGDQTVRQLISGGFEGPIYPVNPGYR
                     TIAGLTSYPTIGDIGEPIDVAVLAVSNANLESEAERAVAAGARSLVIFASCHGRAADG
                     SPLRERVTSIVNEAGIPVCGGNGMGLLNVEAGLRLCGFFQPPGLRSGGISFL"
     gene            556..1164
                     /locus_tag="VFZ80_06855"
     CDS             556..1164
                     /locus_tag="VFZ80_06855"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6287188.1"
                     /translation="MTESRRHDILSATVTAIAERGLCETRISDVAGIVGVSPALVLYY
                     FPTKAALLTEALVYLDRLFYESVAAKLEEASSAEDRLVILIEESCPVPAKPGELPDSW
                     ILWPAMWEMARLDPALAAARARLDEAWRAQIVAIVEDGIANGEFGEVDVKAFTLQLAA
                     MLDGLAIEAMLGDPTVDAARMSELARSFARSSLKGPFSRHRR"
     gene            1211..>2458
                     /locus_tag="VFZ80_06860"
     CDS             1211..>2458
                     /locus_tag="VFZ80_06860"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6287189.1"
                     /translation="MAYHPRENWNPGRRKRVYTRRDFLQRAAVLGIAVPALPSILAAC
                     AEREGGEGAELAVGTPANPVQQPLFEDNPAIESGLEPEAGPLRLYNWEDYINPEIIPL
                     AAEALGVEIEETTFFNEEEALSRLSSGEVSYDVWFPTSQRVPVAVAANLIQPINHDYL
                     PNLSANVWPQLADPYYDQGSRYTAPYVLYTTGIGWRTDMVDSADIEGQENPWDVFWNP
                     DYAGVMGLLDSFTDTIALALFRNGVADPSQATEADLEAAGDALVELVDLMNVRYTIDG
                     AYAGIPEGRFALHHAWSGDLVNSQYYWPEDGDYTVTRYLWPASSEGSTVNGPISNDTM
                     AIPVNAERPVLAHQFINFMLDADNSLLNFEWVGYQPPQNQITPDFLIENEYAADFLRP
                     ALVTPEDFELDSAWVQGPLDAETE"
BASE COUNT          456 a          794 c          775 g          433 t
ORIGIN      
        1 aggaagctga taccgccgct cctcaagccc ggcggctgga agaagccaca gagacggagg
       61 ccggcctcga cgttgagtag tcccatgccg tttcccccac agaccgggat gccggcttcg
      121 ttgacgatcg aggtgacccg ctcgcgcagc gggctcccgt cggccgccct tccgtggcag
      181 ctggcgaata tcacgagcga tcgagcaccc gcagctacag cccgctccgc ctcgctctcg
      241 aggttggcat tggagacagc aaggacggcg acatcgatcg gctctccgat gtcgccgatc
      301 gtcggatacg aggtgagccc ggcgattgtc cggtatccag ggttgaccgg gtagatgggt
      361 ccttcgaaac caccggagat gagttgtcga accgtctggt cgcctacgga gccgcgacgt
      421 tccgaggcgc cgaccacggc cacggaacgg ggttggagca ttggatccag tggatgcatc
      481 tcgacctttg aactgccgtt cagggtaacc gcgggcgcat tgtggcatga tcaggtggtg
      541 agcccgcgtc cgagaatgac cgagagtcgg aggcacgaca tcctctccgc cacggtcacc
      601 gccatcgccg aacgcgggct gtgcgagacg cgcatatcgg atgtggccgg catcgtcggg
      661 gtgagcccgg cgctggttct ctactacttc cccacaaagg ccgctctgct caccgaagcc
      721 ctcgtctatc tggaccgact gttctacgaa agcgtcgccg ccaagctcga ggaggcatcg
      781 agtgcggagg atcgactggt catcctgatc gaggagagct gtccggtccc tgcgaaaccg
      841 ggtgaattgc ccgatagctg gatcctgtgg ccggcgatgt gggagatggc ccgcctcgac
      901 cctgctctgg ccgcggcgcg ggcccggttg gacgaggcct ggcgcgccca gatcgtcgcg
      961 atcgtcgagg acgggatcgc caatggtgag ttcggcgagg tcgacgtcaa ggccttcaca
     1021 cttcagctcg ccgccatgct cgacggtctc gctatcgagg cgatgctcgg agaccccacg
     1081 gtcgatgccg cccgtatgag cgagttggcc cggtctttcg cacgatcttc tctcaagggt
     1141 ccatttagcc gtcatcgacg ttgaacggcc gtttaggatt cggcacccag cgtcgaacga
     1201 ggagcgaacg atggcctatc acccgaggga gaactggaac ccaggaaggc ggaaaagggt
     1261 ctacacccga agggatttcc tgcagagagc ggcggttctc gggatcgcgg tgccggcatt
     1321 gcccagcatt ctcgctgcct gcgccgaacg ggaagggggt gagggagcgg agctcgctgt
     1381 tggcaccccg gccaacccgg tgcagcagcc gctcttcgag gacaaccctg ccatcgagtc
     1441 gggtctggag cctgaggccg gtcctctacg cctgtacaac tgggaggact acatcaatcc
     1501 cgagatcatc ccgctggctg cggaagccct cggggtcgag atcgaagaga ccacgttctt
     1561 caacgaagag gaagctctga gcaggttgtc ctccggtgag gtaagttacg acgtctggtt
     1621 ccccacgtca cagcgcgtcc cggtggcggt tgccgccaac ctgatccagc caatcaatca
     1681 cgactacctg cccaatctgt cggccaacgt gtggcctcag ctcgcagatc cctattacga
     1741 ccagggctcg cggtacacag ccccatatgt gctctacacc accgggatcg ggtggcgcac
     1801 cgacatggtg gacagtgccg acatcgaggg ccaggagaat ccgtgggacg tgttctggaa
     1861 cccggactac gccggtgtca tgggtctgct cgactcgttc accgacacca tcgcattggc
     1921 cttgttccgc aacggcgtcg ccgacccgtc acaggccacg gaggccgatc tagaggcggc
     1981 gggcgatgcc ctggtcgagc tggtcgacct gatgaatgtc aggtacacca tcgacggcgc
     2041 ctacgccggc atccccgagg gtcgcttcgc actacaccat gcctggtcgg gagacttggt
     2101 gaactctcag tactactggc ccgaggacgg cgactacacg gtgactcgct acctatggcc
     2161 ggcgagctcg gaggggtcga ccgtgaacgg tccgatctcc aacgacacca tggccatccc
     2221 cgtgaacgcc gagcggccgg ttctggccca tcagttcatc aacttcatgc tcgacgccga
     2281 caactcgctc ctcaacttcg agtgggtcgg ctaccagcca ccccagaacc agatcacgcc
     2341 cgacttcctc atcgagaacg agtacgccgc cgatttcctt cgaccggcac tggtcactcc
     2401 cgaggacttc gagctcgaca gcgcctgggt gcagggcccc ctcgatgccg agaccgag
//
LOCUS       DASUTA010000342         2568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_102075, whole genome shotgun sequence.
ACCESSION   DASUTA010000342 DASUTA010000000
VERSION     DASUTA010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102075"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            600..2003
                     /locus_tag="VFZ80_06865"
     CDS             600..2003
                     /locus_tag="VFZ80_06865"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEX6287190.1"
                     /translation="MTFALFLVVVMITSWLLRPRHRIWKWWLLVASWVFYAWWDWRFV
                     LLLVAVIGTTHLAATAMDRWPRSQRTWLALGIAADIAILAFFKYYGFFVTSWLELLGT
                     VGLSPEVPLFEVILPIGVSFYTFESIAYLVELRRGVVERMRLLDLATYLSFFPKLLSG
                     PITRASEFGPQLNEPTAASELDGTTAFWLLGRGLFKKMVIAGYLAAAITDGVFTTPGQ
                     YSSLEVLAGIYGFAALIYIDFSAYTDMARGVAILLGFRLPENFRSPYVASSVREFWTR
                     WHMTLSRWLRDFLFQPLVISNSSGRLVRMANLMIVMLLAGLWHGAAWTFVLWGGIHGA
                     GLAWERLRREHRRSRGEKPIPVTRWVTLRRRFLTFQFVAFAWVFFRADSVSAAFDVFA
                     RLGSFGPAPAVTPLLIVVLVAVFALQYFPEGWSTRMYAGFRRVGPVAQTLALGGLLLV
                     VDAFGPDGVPAFIYTRF"
     gene            2098..>2568
                     /locus_tag="VFZ80_06870"
     CDS             2098..>2568
                     /locus_tag="VFZ80_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287191.1"
                     /translation="MLVLVGALLLGGILNGHSLLDTASALEPGWQRNVATRAAQLLVA
                     AAETLHLDNPRDWIDRQLGRGPKPTVPLVIPPLATTTSTTSTTVATTITTTPAVTTQP
                     PQTTSTLPTTTTLPGVFIPTAERPVRLWVTGDSLTERFGPALVNLLGDTGVVEAR"
BASE COUNT          421 a          854 c          762 g          531 t
ORIGIN      
        1 ggcgcactca agtggagcca ggagggtgac caacctgtcc cggatctgga aagtctggca
       61 cgtcgccggc gtctcccgct accgatgaaa ccgccgtata tgcagccgca agagctgatc
      121 tcgctggctt ggagtggagt ctctctgctc atggcctggc gagcttcgct ggacgtccgg
      181 tgccgcggcc tcggttctga acccaacgta aaccccacgc cgtcgccgac ggtgtgatgt
      241 atgtagtgac cgggaaccgc catctctagc catcgacatg ttcccttacc ctggtgcccg
      301 cgtcctccgg gtcctggacc acagaacaac gcttgatcgg gccgactgtt ccacagtcgt
      361 tcacaaaccg ccaagcatcg gcgggatctg gacgcagctg gtcgtcgcgc ccagcccgat
      421 tgatccgctg caccacttcg tgaggcggga ggtttcgagc cgaagtgagg ttctgaggtc
      481 gacaaaaggc ccaaacaagg acccatccac tttctgacca tcggccctgg tgacaacgac
      541 ggacacgcaa gacactcgga ctgttagcaa gggaaggaag acgattgcca ttcccgaccg
      601 tgaccttcgc gttgttcttg gtagtcgtga tgatcacgtc gtggctgctt cgcccacgcc
      661 accgaatctg gaaatggtgg ctgctcgtcg ccagctgggt cttctacgcg tggtgggact
      721 ggcgattcgt gcttctgctg gtggcggtga tcggcacaac tcatctggcg gcgacggcca
      781 tggaccgatg gccgcggtcg cagagaacgt ggctcgcgct cggcattgcc gccgatatcg
      841 cgatcctcgc ctttttcaag tactacgggt tcttcgtcac ctcgtggttg gagctactgg
      901 gcaccgtcgg actatctccc gaagtcccac tcttcgaagt catcctcccg atcggcgtct
      961 ccttctacac cttcgagtcc atcgcatacc tggtcgagct gcgtcgtggt gtcgtggagc
     1021 gaatgcgtct tctcgatctg gccacctacc tcagcttctt ccccaagttg ctctccgggc
     1081 cgatcacgag ggccagtgag tttggccccc agctgaatga gcccaccgcc gcctccgaac
     1141 tcgacggaac gacggcgttc tggcttctgg ggcgtggatt gttcaagaag atggtcatcg
     1201 ccggctatct ggcggccgcc atcacagatg gcgtcttcac cacccctggt cagtattcga
     1261 gcctcgaggt cctggccggc atctacgggt ttgccgcact catctatatc gacttcagcg
     1321 cgtacaccga catggcccgc ggcgtggcga tcctcctcgg attcaggctg ccggagaact
     1381 tccgcagtcc atatgttgcc tcttcggtaa gggagttttg gactcgttgg cacatgacgc
     1441 tgagtcgctg gttgcgggac ttcctgttcc agcccctggt catttcgaat tcgagcggcc
     1501 gactcgtgcg tatggccaac ctcatgatcg tcatgcttct cgccggtctc tggcatgggg
     1561 cggcgtggac gttcgtcctc tggggcggca tccacggcgc cggcctggcc tgggagagat
     1621 tgcgacgaga gcaccggagg agccgggggg aaaagcccat cccggtgact cggtgggtaa
     1681 cgctgcggag gagattcctc acgtttcagt tcgtcgcctt cgcctgggtg ttcttccggg
     1741 ccgactccgt ttcggccgcc tttgatgtct ttgctcggct cggctcgttt ggacctgctc
     1801 ccgcggtgac tccgctattg atcgtggtcc tcgtggccgt gttcgccctc cagtacttcc
     1861 ccgaaggctg gtcgaccagg atgtatgccg ggttccgccg ggtgggtccc gtcgcacaga
     1921 cactggccct cggaggctta ctcctggtgg tcgatgcctt cgggcccgat ggcgttcccg
     1981 ccttcatcta tacgaggttc tgagatggct tcctcaggac cctcgacgct cgaagcccgg
     2041 accgcggcga ggcacccaag acacatcgcc aagcgggcac cgttcccatg gtcacccgtg
     2101 ctcgttctcg tcggagcatt gctgcttggc gggatactca acggacactc gctgctcgac
     2161 acggcctctg cactcgaacc cgggtggcaa cgcaacgtgg cgacacgagc cgcccagctt
     2221 ctggtcgccg ccgccgagac gctgcacctc gacaatcccc gggactggat cgatcgccag
     2281 ctgggacggg gacccaagcc caccgtgccc ttggtcattc cccctttggc gaccaccacg
     2341 agcaccacga gcaccaccgt cgcaacgacg atcacaacca ccccggcagt caccacccag
     2401 cctccacaga cgacctcgac cctccccacc accaccacgc tgcccggcgt gttcatccca
     2461 accgcggaga ggccggtccg gctgtgggtt acaggtgact ctctcaccga gaggttcggg
     2521 ccggctctgg tcaatctcct cggcgacacc ggcgtagtcg aggcgagg
//
LOCUS       DASUTA010000343         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_876916, whole genome shotgun sequence.
ACCESSION   DASUTA010000343 DASUTA010000000
VERSION     DASUTA010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876916"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VFZ80_06875"
     CDS             complement(<1..353)
                     /locus_tag="VFZ80_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287192.1"
                     /translation="MSDKPRTTSVVIRRAFAEAPSLGEGLKLTLVLAMTGQAVTVITP
                     IVLQILIDEQINNPAGVSMAGVLQLAAIALAALVVGVIVGRISLLRLVNTSSTGLSDL
                     RIKTFGHIMNQSVLHV"
     gene            complement(350..2137)
                     /locus_tag="VFZ80_06880"
     CDS             complement(350..2137)
                     /locus_tag="VFZ80_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6287193.1"
                     /translation="MSDRAMTPTAVLRDGVKLIGRFVKMHPMAFALAVFGAAAYAGAI
                     LLSSWVIGWATDNVIIPVLDEGQEPDLLVMAGLVILGVAVFKAAGIVLRRTSAAYLTG
                     ATRRDIRNRLLAHQLTLKMSWFNRQAIGDLLAVADSDTDQGTGVLGPLPYATGVSLLL
                     VGTIVLIASIDIWLGVGAVIGLAVAIVIDIHGSWVTFSLWEDVQKHRGIVSSIAHESF
                     DGALTVKALGRESHVSDQFGVASDELRDRIITVNSTWTKYQVIMRALPQMLIIVLLVV
                     GAARVTVGAVSAGDIVTIAYLLTLLAFPIQLIGFVLWDLAGSLAGWRRVQVVLDADDH
                     VVWGSRSASMETGAAPVSGRTVGFAYDDDPVLSELDLDLEAGTTLAVVGPTASGKTTL
                     GLLLARLWDPDSGSIRIEGRDLRQFARSELPKEVAYVAQNAFLFDASVRENITLDGVF
                     SDDEVARALRLAGADEFVSQLPNGLETELGERGTTLSGGQRQRLALARALIRKPRLLI
                     LDDATSAVDPSVEAEILRALKSAELPSTIVVVAYRPSSIRLADEVVYVDQKRILAHGK
                     HDDLIESEPGYARLVKAYEEEARRLREAS"
BASE COUNT          458 a          719 c          708 g          363 t
ORIGIN      
        1 acatggagca ccgactggtt catgatgtgc ccgaatgtct tgatgcgcag gtcggagagg
       61 ccggtcgacg aggtgttcac cagacgcaac agcgatatgc ggcccacgat gacgcccacc
      121 accagggccg ccagcgcaat ggcggcgagc tgaagcaccc cggccatgga gaccccggcc
      181 gggttgttga tctgctcgtc tatcaggatc tggagaacga tcggtgtgat gacggtcacc
      241 gcctggcccg tcatcgccag caccagtgtc agcttcaatc cctcacccaa cgaaggcgcc
      301 tcggcgaagg cgcgtctgat gacgacactg gtggtgcggg gcttgtcgct catgacgcct
      361 ccctcaaccg acgagcctct tcctcgtatg ccttgaccaa tcgggcatag ccgggctctg
      421 actcgatcag atcgtcgtgc ttcccgtggg cgaggattcg cttctgatct acgtacacga
      481 cttcgtcggc gaggcgaatc gacgacggcc ggtaggcgac cacgacgatc gtcgagggaa
      541 gctcggcgct cttgagtgcc cggaggatct cggcctccac cgaggggtcg acggccgacg
      601 tcgcatcatc gaggatcaac aaccttggct tccggatcag agcgcgtgcc agggcgagac
      661 gctgcctctg acctccagac aacgtcgtgc cccgctcccc cagttcggtc tccagcccat
      721 ttggcagctg ggagacgaat tcgtccgcac cggcgagacg aagggcacgg gccacctcat
      781 cgtcagagaa gacaccgtcg agggtgatgt tctcccgcac cgaggcgtcg aacagaaatg
      841 cgttctgtgc gacataagca acttccttcg gcaactccga tcgggcgaac tggcgcagat
      901 cccgtccctc gatgcggatc gaccccgagt caggatccca aagccgcgcc agcagcaaac
      961 cgagtgtggt cttgcccgat gccgtggggc cgacgaccgc cagggtggtg ccggcctcca
     1021 ggtcgaggtc gagctcgctg aggacgggat cgtcgtcata ggcgaatcca acggttcgac
     1081 ccgagaccgg cgctgcgccg gtctccatgc tggccgagcg cgagccccac acgacgtgat
     1141 catcggcgtc gagcaccact tggacgcgcc gccatccggc caacgaccct gccaggtccc
     1201 agagcacgaa cccgatcaac tggatgggga atgcgagaag cgtcaacagg taggcgatgg
     1261 tcacgatgtc gccggctgac acggctccga ccgtgacccg ggcggcgcca accacgagca
     1321 ggacgatgat cagcatctgg ggcagggcgc gcatgatcac ctggtacttg gtccacgtcg
     1381 agttgactgt gatgatccgg tcccggagct cgtcgctggc gacgccaaac tggtcggaca
     1441 catgggactc ccggcccagg gccttgaccg tcagggcacc gtcgaagctc tcatgtgcga
     1501 tcgacgagac gatcccacgg tgcttctgaa cgtcctccca gaggctgaag gtgacccaac
     1561 tgccgtggat gtcgatcacg atcgccaccg caaggccgat caccgcaccg acaccgagcc
     1621 agatgtctat cgatgcgatc aagacgatcg tccccaccag cagcaacgac acacctgttg
     1681 cgtatggaag ggggccgagg acgcccgtcc cttgatcggt gtcggaatcg gccacggcga
     1741 ggagatcgcc gatggcctga cgattgaacc aggacatctt gagggtcagc tggtgggcga
     1801 ggagccggtt gcggatgtct cggcgggtgg cacctgtcag gtatgccgca gaagtgcgtc
     1861 tcagcacgat gcccgcggcc ttgaacaccg cgacgccgag tatcaccaga cccgccatga
     1921 ccaggagatc cggctcttga ccctcatcga ggaccgggat gatcacgttg tcggtcgccc
     1981 acccgatgac ccaggaggag aggaggatgg ccccggcata tgcggcagcc ccaaagacgg
     2041 cgagcgcaaa ggccatcggg tgcatcttca cgaagcgccc gatgagcttc acaccgtcgc
     2101 gcaggacagc agttggggtc atggcacggt cagacatcac aaaccaaggt agaacttgaa
     2161 gaacggttta agtcaagccc gtcgccgcat cgggctgggt cgacatgata cgagatgccc
     2221 ccaggagacg tcgacgaata atccctag
//
LOCUS       DASUTA010000344         3049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1656073, whole genome shotgun sequence.
ACCESSION   DASUTA010000344 DASUTA010000000
VERSION     DASUTA010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656073"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VFZ80_06885"
     CDS             complement(<1..275)
                     /locus_tag="VFZ80_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287194.1"
                     /translation="MSQLRSVIDDFMSFDPDDASAEELAASIREAIHGQQMLHTAISG
                     WVKTLADRGDHHSLGYSSPSAFLCEQTGMSPGTAKRMVSHGNAKTRAP"
     gene            486..746
                     /locus_tag="VFZ80_06890"
     CDS             486..746
                     /locus_tag="VFZ80_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287195.1"
                     /translation="MSQKSDPPDIHSSKVTERIEATALELLDANPDGIRWADLNRKIE
                     ESDPSFHPKTINGCVWRLIEKYPDRVYKPSKGLFRLMKYRAR"
     gene            complement(764..2086)
                     /locus_tag="VFZ80_06895"
     CDS             complement(764..2086)
                     /locus_tag="VFZ80_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX6287196.1"
                     /translation="MRLGAVILALVCAAAASGCGGSVGANDWTLIGMTPDERHLLVTT
                     LFGGVVSDCTRWKGWEVDESADRVEVRALLWRRTLPSGCTTEGVIETIEVDLDEPLGD
                     RPLVGCGSDDCLGFEFDSAFGPWIVDVAPTSAGVAVTNDGALWGFGGDGEMIWNHDPG
                     SADLLQGRFDVVVAYDGGATSGYDPLTGERIWRVDGYFAAADDENVYVCSGEDAGVVS
                     AIEPGTGAERWQTNLPCGLVAAGEDVVTIVAIDPNVDGGHELLLVDPATGEVLLREKF
                     DDGTDDRVNGFEGAVLVGDDVVVSGIQADFVVLDNEGNEVTREPGAIGTPIGQVEGLV
                     ILASHDGVTAVDPGSGEIVWESVDFNHESLAVSEETLWALEASTGGVSRLDPRSGQPL
                     WTAPVGITSGFDIATDGPIAYVATSTAVIALDSATGELVWWKHVPFEG"
     gene            complement(2149..>3049)
                     /locus_tag="VFZ80_06900"
     CDS             complement(2149..>3049)
                     /locus_tag="VFZ80_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287197.1"
                     /translation="STSTTSAATTTTGVSPEIAEIGYPVSDEWVVETVARDIDAGTGG
                     LAVDDEGVIYMGDFGYTTNTGNGIYRITPDGEVTVFAGADEMSQLTTTKIGPDGSLYQ
                     SGYGSNTVFRVDGEGIATVVTDDILGPTGIVALEDGTLVVASWSQGIIHRVHPDGTVE
                     DWVAPHPGFNGVNSLTMGPDGTIYLVNLRDGALFAIDPEGAVSELHFFPRANSHVVYH
                     DGSLFITSRGDYVIFRYDLTTREAEIVAGNGEPGDADGRGVESSFGRPNAIAVGPNGA
                     LYFNHTEGTGDTPVHIKRLTYQP"
BASE COUNT          581 a         1000 c          895 g          573 t
ORIGIN      
        1 ggggctctgg tcttggcgtt gccatgagag accatccgtt tcgccgtgcc cggtgacatg
       61 ccggtctgct cacacagaaa cgccgatggc gacgagtacc ccaacgagtg gtgatcacca
      121 cgatccgcca aggtcttcac ccatcccgaa atggcggtgt ggagcatctg ctggccgtgg
      181 atcgcctctc tgatcgaagc ggccaactct tcagccgagg catcgtccgg atcgaacgac
      241 atgaagtcat cgattacgga acgcagctga ctcatctagt tcccctcgtc ctgtcaccaa
      301 cgtacatgcc gtcaatgaca gatttcctct gtcaacaccc tcactcatca acctacgacc
      361 ccggtgtgac aagaattcga ccatgagctc ggggtcgctg atcagagcag aagtcgtgag
      421 gggcgacgtc gagcttccga ctgcgtttca gcttcctgga cccgagtcac gatggagccc
      481 caccgatgag ccagaagtct gatccgcccg acatccattc ttccaaggtc accgaacgca
      541 tcgaggccac ggcattggag ctgttggatg cgaaccccga cggcattcgg tgggctgatc
      601 tcaaccggaa gatcgaggaa tcagacccga gcttccatcc caagacgatc aacgggtgtg
      661 tttggaggct gatcgagaag taccccgaca gggtctacaa accgtccaag ggcctgtttc
      721 ggctgatgaa atacagggcc aggtgagggt cagcgacgat gcgtcaaccc tcgaaaggca
      781 cgtgcttcca ccaaacgagc tcaccggtcg cgctgtccag agctatcacc gctgtcgagg
      841 tagcgacgta cgcgatgggg ccgtcggtcg cgatgtcgaa gccgctcgtg atgccaactg
      901 gcgccgtcca gaggggttgg ccggatcggg ggtcgagccg tgacacgccc cccgtcgagg
      961 cttcgagggc ccacagagtc tcctcggaga ccgcaagcga ctcgtggttg aagtcgactg
     1021 actcccaaac gatctcgccg ctcccagggt cgacagcagt gacaccgtcg tgggaagcca
     1081 ggatcaccag gccttcgact tgaccgatcg gtgttccgat cgctccaggc tctcgggtta
     1141 cctcgttgcc ttcattgtcg agcaccacga aatcggcttg gatccccgac accaccacat
     1201 cgtccccaac gagaactgca ccctcgaagc cgttgacccg gtcatcggtc ccatcgtcga
     1261 acttctctcg caggaggact tccccagttg ccggatcgac cagtaacagc tcgtgtccac
     1321 cgtcgacatt cggatctatc gccacgatcg tgaccacgtc ttcgccagcg gcgaccaacc
     1381 cacagggcag gttcgtctgc cagcgctcgg cacctgtccc gggctcgatg gccgacacga
     1441 cgccggcatc ctctccagag cacacgtaca cgttctcgtc gtcggcggct gcgaagtacc
     1501 catccacccg ccagattctc tctccggtga gcgggtcgta cccggacgtg gctccgccgt
     1561 cgtacgccac gacgacatcg aagcggccct gcaggaggtc ggccgacccg gggtcgtggt
     1621 tccagatcat ctcaccgtcg cctccgaagc cccatagtgc tccgtcgttc gtcacagcga
     1681 caccggccga cgtgggagcc acgtccacga tccagggacc gaatgcagaa tcgaattcga
     1741 atccgagaca atcgtcgctg ccacagccaa cgagtggccg atcgccgagc ggctcgtcca
     1801 ggtcgacttc gatcgtctcg atgacaccct cggtagtgca gcccgacggc agcgtcctgc
     1861 gccagagcag ggcccgcact tcgacgcggt cggccgattc gtccacctcc caacccttcc
     1921 agcgtgtgca gtcgctgaca acgccgccga agagtgtcgt gaccaggaga tgtcgctcat
     1981 cgggtgtcat gccgatcaga gtccagtcgt tggcgcctac cgagccgcca catccggagg
     2041 ctgctgccgc gcagaccaga gccagaatca ctgcaccaag ccgcatccat gatgtttgac
     2101 gtctgacgcc ggattcgggt ttcctccact gcacctgatc gtcgcaggtc agggttggta
     2161 tgtcagtctc ttgatgtgga ccggcgtgtc gcccgtacct tccgtgtggt tgaagtagag
     2221 cgccccgttc ggcccgacag cgatggcatt gggccgaccg aacgacgact ccacgccgcg
     2281 gccgtccgcg tcgcccggtt caccgttgcc ggcgacgatc tcagcctccc tcgtcgtcag
     2341 gtcatagcgg aagatcacgt agtcgccacg gctggtgatg aacaacgagc catcgtgata
     2401 gacgacgtgg gagttcgccc tggggaagaa gtgcagctcc gagactgcac cttcaggatc
     2461 gatggcgaac aatgctccgt cacggagatt gaccagatag atcgtaccgt cgggccccat
     2521 ggtcaggctg ttcactccat tgaaaccggg atgtggcgcc acccagtcct ccacggtgcc
     2581 gtcggggtgg acccggtgga tgattccttg gctccacgag gcaaccacca gcgtcccatc
     2641 ttcgagagcc acgattccgg ttgggccgag gatgtcgtcg gtgaccaccg tcgcgatgcc
     2701 ctcgccatcc actcgaaaga ccgtgttcga gccgtagccg ctctgatata ggctgccgtc
     2761 ggggccgatc ttggtcgtgg tgagctgact catctcgtcg gcaccggcaa acacggtgac
     2821 ctcaccgtct ggggtgatcc tgtagatccc gttgccagtg ttggtggtgt acccgaaatc
     2881 gcccatgtag ataacgccct catcgtcgac agccaggccg ccggtgccgg catcgatatc
     2941 gcgggcgacg gtctctacga cccattcgtc ggagaccggg tagccgatct cggctatctc
     3001 tggcgagacg cctgtcgtgg tcgttgccgc cgaggtggtg gatgtcgag
//
LOCUS       DASUTA010000345         5781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_620094, whole genome shotgun sequence.
ACCESSION   DASUTA010000345 DASUTA010000000
VERSION     DASUTA010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620094"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /locus_tag="VFZ80_06905"
     CDS             <1..132
                     /locus_tag="VFZ80_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014987713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEX6287198.1"
                     /translation="LPFTSSFMALGDGTHKLPVRADVQRAIGKGPGDDVLIELTERL"
     gene            complement(158..1081)
                     /locus_tag="VFZ80_06910"
     CDS             complement(158..1081)
                     /locus_tag="VFZ80_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX6287199.1"
                     /translation="MSQNFYEVLGVPRNASQGQVLDAYFSRLRGAHPDVSVSSDHHVV
                     RDRYEAFMVLSDSATREKYDRALAGAELCPWCGNPLAAYGLDQHVADHVVKDASEGCI
                     VCGRLPARHFRFKANTGLVVWRKVDQVDGNLCKTCATGVYRAMQTRNLSRGPWSIISI
                     FTTPVDLVRNWVSHRKAASLAGPRPHDQSYDRGPGLGRPVLRNGSVWASLLGISVLIL
                     ATAFLIQSNDTPSPIPDAEVSQTTTTIDPNDGWVVGGCAEFDMAGRVFPVECGDHFAE
                     VVALVSTTAECPESTDFSVPLTEGVACFEES"
     gene            1169..2746
                     /locus_tag="VFZ80_06915"
     CDS             1169..2746
                     /locus_tag="VFZ80_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287200.1"
                     /translation="MTTVAQRSIRIGERKIPVVLPNRRDARLHTASVILSIHTIGILF
                     LGFEVSVPQIISAIATAALIDAGLTYYRSGSLVWPASGMLTGSGVALILRYVDTNAGD
                     YWSWSGWYWFTAVAGVSVLTKYLVRWRGSHIFNPSNVGLVAAFLIAGSGLIEPLDFWW
                     APLDFWMVLAYALIIGGGMLITRRLRLLETALVFWVVLVTGVGVLAASGHCMITTWST
                     TPVCDSRFFTTLATSPEVLIFLFFMITDPKTIPERRTARVVFAGTLAIVATLLMAPQS
                     LEYGAKVALLGSLVFLSPLRWLFDRMFAGFHERGSGLADLMRRLAPSGRPVLTFSRGL
                     ALGSISVLVAIAIVAAGAPARDAALAAQPQSPQDIPVQVDPGELPEVAIDESTRQLDI
                     TIDESAAQQLALMLAENLALEAEAIRTADAELLALADGGERLTELQARIDDAITSGDR
                     VADHYELESLEVRAAGESEGQSSAALAFDAGGVVTTVAYDPSGQELDSSTHEFDTTFV
                     MRQLAGERWLIVIEIDD"
     gene            complement(2751..4187)
                     /locus_tag="VFZ80_06920"
     CDS             complement(2751..4187)
                     /locus_tag="VFZ80_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEX6287201.1"
                     /translation="MGARIGPQPLARFGRLKDMEQDRRIRLANVELPDFGMPSTIPSL
                     PASMYEQRLSELARRADEAGHDIVVVYADREHSANMSYLTGFDPRFEEALLIVEPDRD
                     PILLVGNECVGIAKAAPLAMDVRLFPGLSLPGQPDPVGLDLTEALSDAGVARGTRVGA
                     IGWKEMESADRLDLPAFVVDDLRQLTGDVDLVRNATRLLIDPADGMRVINEVEQLAYF
                     EYAACQTSSGVRRLLLELEPGMSEQEAVQLLRWNGAPLSCHLMLTAGERARLGLLSPG
                     DRAIERGDPLTVAFGIWGALNCRAGFVVASESQLPVDVSDYVDRLVAPYFGAIVEWYE
                     ALRVGQTGRVLWDIVARRLGDPFFGIFLNPGHQIHLDEWVNTPVRPNSEIELRSGMAL
                     QVDVIPATGTSYFTTNIEDGIALADRDLRERFARSYPDAWDRIQARRAFMRDVIGIDL
                     HADVLPFSNIPAFLPPLLLDAGRAMTAA"
     gene            complement(4139..>5781)
                     /locus_tag="VFZ80_06925"
     CDS             complement(4139..>5781)
                     /locus_tag="VFZ80_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006939080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEX6287202.1"
                     /translation="AWSSTLWLVLGLVVGAGVTMGAVMAAAAWLSEPTGGEALEPPLF
                     VEVGDIAGIVHRYDGEFEYFVGGGVATFDCNSDRLPDLYLAGGSRPAALYVNETVPKG
                     DLSFVSQPGSETDLVGVTGAYPIDIDSDGHMDLVVLRRGENVVLRGTGRCQFERANET
                     WNIQGGDEWTVAFSAFWESGEELPTLAFGNYLRIQGSGDRDECEDHFLFRPADADSYA
                     GPETLSPGHCTLSILFSDWNRSGSPDLRMTNDRHYYTEGEEQLWEIDDGARRYGAGDG
                     WRHLKIWGMGIASQDLDGDLLPEVFLTSQGDNKLQTLVGDGSRPEYEDIALASGVTAH
                     RPFQGDNLRPSTAWHAEFDDVNNDGLWDLFVTKGNVDAQIDFAAEDPNNLLIGQRGGL
                     FAERAAEAGLLDMNRSRGAVVVDLDLDGALDVVVVERRVPARIWQNTGQTSSGGSVGN
                     WLSLEVGQPGWNRNAVGSWVEIQSDDGVVTREMTVGGGHASGEMAPIHFGLGDRAEAR
                     VRVIWPDGETTPWRTLPANGRFSWERGSDPNPLPGSAD"
BASE COUNT         1040 a         1910 c         1783 g         1048 t
ORIGIN      
        1 ctccctttca ccagttcgtt catggcgcta ggtgacggaa cgcacaaact ccccgtgagg
       61 gccgacgtgc agagagccat cggcaagggt cccggtgacg acgtgctgat cgagttgacc
      121 gagcgtctct gactcgatcg cagcagcgcc cgacccgcta ggactcctcg aagcaggcca
      181 cgccttcggt caacggcacc gagaaatcgg tgctctccgg gcattccgcc gtcgtcgaca
      241 ccagggccac gacctctgcg aagtgatcac cgcattccac ggggaagacc cggcccgcca
      301 tgtcgaactc ggcgcaaccg cccaccaccc agccgtcgtt cgggtcgatg gtggtggttg
      361 tctggctcac ctccgcgtcc ggaatcggtg aaggcgtgtc gttgctctgg atcaggaacg
      421 cagtagccag aatcagaacc gagatcccca agagagaagc ccataccgaa ccgttccgta
      481 ggacagggcg gcccagaccg gggccccggt cgtaggactg gtcgtgaggt ctggggccgg
      541 ccagcgaagc cgccttccga tgagagaccc agttgcggac cagatcgacc ggagttgtga
      601 agatcgagat gatgctccaa ggtccccggc tgagattcct cgtttgcatt gcccgataca
      661 cgccggtggc gcaggtcttg cacaggtttc catcgacctg atcgaccttg cgccagacca
      721 ccaacccggt gttcgccttg aatcggaaat gtcttgcggg gagtcgaccg caaacgatgc
      781 agccctcgct ggcatccttg accacatggt cggccacgtg ttgatcgagg ccataggcgg
      841 cgagggggtt cccgcaccaa gggcacagct cggcaccggc caaggcacgg tcgtacttct
      901 ccctggtggc agagtcggac agcaccatga acgcctcgta tcggtccctc accacgtggt
      961 ggtcactgct cacactcacg tcgggatgcg cccctcggag ccgtgagaag tatgcgtcga
     1021 ggacctgtcc ctgagacgcg ttcctcggca cccccaatac ctcgtagaag ttctgactca
     1081 tcggctcctg tgcctgagac ggcgggtggg caagttacgc cagtccagct cagacaccaa
     1141 gctcgacgtg acttctaacc tcgtcccgat gacgactgtg gcccagcgat cgatccgtat
     1201 cggggagcgg aagataccgg tcgttctccc caatcgacgc gatgccaggc tccacacggc
     1261 gtcggtgatt ctcagcatcc acaccatcgg gatcctcttc ctcggattcg aggtctccgt
     1321 accccagatc atctccgcca tcgccaccgc tgccctcatc gacgccggac tcacctatta
     1381 ccgctccgga agtctcgttt ggccggccag cggaatgctc acgggaagcg gtgtcgccct
     1441 catcttgcgc tatgtcgaca ccaatgcagg cgattactgg tcgtggtccg gctggtactg
     1501 gttcaccgct gttgccggag tctcagttct cacgaaatac ctggtccgtt ggcggggcag
     1561 tcacatcttc aatccctcga atgtggggct ggtcgccgca ttcctgatcg cgggcagcgg
     1621 gctgatcgag cccctcgact tctggtgggc tcctctcgac ttctggatgg tcctcgccta
     1681 cgccctgatc atcggaggtg gcatgctgat cacccggcgg ctccggctgc tggagacggc
     1741 tctggtcttc tgggtcgtcc tagtgacagg tgttggggtc ctggccgcgt ccggtcactg
     1801 catgatcacc acctggtcga ccacaccggt gtgcgactct cgcttcttca ccaccctggc
     1861 gacttcgcca gaggtgctga tctttctctt cttcatgatc accgacccca agacgattcc
     1921 agagaggcgg acggctcgtg tggtgttcgc gggcactctg gccatcgtgg ccaccctgtt
     1981 gatggctccg cagtccttgg agtacggagc caaagtggcc ctgttgggga gcctggtctt
     2041 tctgagcccg ctccgctggt tgttcgatcg catgttcgcg ggcttccacg agcgaggcag
     2101 cggcctagcc gacctgatga ggaggctggc tccgagcggt cggccagtcc tcaccttctc
     2161 gagaggtctg gctctcggat cgatctccgt gctggtggcg atcgcgatcg tggccgcagg
     2221 tgcccccgcc cgtgacgcgg cgctcgctgc tcagcctcag agccctcagg acatccccgt
     2281 tcaagtcgac cccggggagc ttcccgaagt tgccatcgac gagtcgaccc gacagctcga
     2341 catcaccatc gacgagagcg cagcccagca gctcgccttg atgcttgccg agaatctcgc
     2401 cctcgaggcc gaggcgatca ggacggccga cgccgaactg ctcgctctgg cggacggtgg
     2461 cgagaggctc accgagctgc aggcgcgcat cgacgatgcg atcacgagcg gggatcgtgt
     2521 tgccgaccac tacgagctgg agagcctcga ggtgagggcg gccggggaat cagagggtca
     2581 gagctcggca gcgctcgcct tcgacgccgg cggagtcgtc acaactgtcg cttacgaccc
     2641 gagcggacag gaattggata gttcgaccca tgaattcgac acgacgttcg tcatgcgcca
     2701 actcgcaggt gagcgctggc tgattgtgat cgagatcgac gactgagcgg tcatgccgcc
     2761 gtcatcgccc tgcccgcgtc gagcaggagt ggcggcaaga aggcagggat gttggagaat
     2821 ggcaggacgt cagcgtgcag atcgattccg atcacgtctc gcatgaacgc gcgtcgagct
     2881 tggatccgat cccaggcgtc ggggtacgac cgggcgaacc gctctcgcag gtctcggtcg
     2941 gccagggcta tgccatcttc gatgttggtc gtgaaatacg acgttccggt ggcagggatg
     3001 acatcgacct ggagggccat gcctgatcgc agctcgattt ccgagttggg acggaccgga
     3061 gtgttgaccc actcgtcgag atggatctgg tggcccgggt tgaggaagat cccgaagaac
     3121 ggatctccca gacgcctggc aacgatgtcc cacaggacgc ggcccgtctg accgaccctc
     3181 aacgcctcat accactcgac gattgcgccg aagtagggag cgaccaaccg gtccacatag
     3241 tcggagacgt cgacgggaag ttggctctcg ctggccacca cgaaccctgc ccggcagttg
     3301 agggcgcccc agatgccgaa ggcgacagtg aggggatcgc ctctctcgat ggcccgatcc
     3361 cccgggctca acaacccgag acgagcacgt tctcctgcgg tcagcatcag atggcacgac
     3421 agaggtgccc cgttccagcg caacagttgc accgcctcct gctcggacat ccccggctcg
     3481 agctcgagga gcagacggcg cactcccgat gacgtctgac acgccgcata ctcgaagtag
     3541 gcgagctgct cgacttcgtt gatcactcgc atcccgtctg cggggtcgat caagagcctt
     3601 gtggcattgc gcaccagatc aacgtctcct gtgagctgtc tcaggtcgtc gacgacgaaa
     3661 gcggggaggt cgagtcggtc cgccgactcc atctccttcc aaccgatggc accgacccgc
     3721 gtccctcgtg ccacgcccgc atcggagaga gcctcggtga gatcgagccc caccgggtca
     3781 ggctgacccg gaaggctcag gcccggaaac agcctcacgt ccatggccag aggcgccgcc
     3841 ttggcaatgc cgacgcactc attgccgacg aggaggatcg gatcacggtc cggctccacg
     3901 atgagcaggg cctcctcgaa acgggggtcg aacccggtga gataggacat gttggcgctg
     3961 tgctcacggt cggcgtagac gacgactatg tcgtgacccg cctcgtcagc cctgcgagcc
     4021 agctctgaca gcctctgttc gtacatggag gcaggcaagg acgggatcgt ggaaggcatc
     4081 ccgaagtcgg gtagctcgac gttcgccaga cgtatccgcc ggtcctgctc catgtccttc
     4141 agtctgccga accgggcaag gggttggggt ccgatcctcg ctcccatgag aagcgaccgt
     4201 ttgccggcag ggtgcgccaa ggggtggtct cgccgtcggg ccagatcacc ctgacccggg
     4261 cctcggctcg atcgcccagc ccgaagtgga tgggtgccat ctcccctgag gcgtgaccac
     4321 cgccaacggt catctcccgt gtgactacac cgtcgtcact ctggatctcg acccacgacc
     4381 caaccgcgtt tcgattccat ccaggctggc cgacctctag tgacagccag ttcccgaccg
     4441 aaccgccgga gctggtctgc cccgtgttct gccaaatccg agccggaacc cgccgttcca
     4501 ccacgacgac atccagggca ccgtcgagat cgagatccac cacaactgca ccgcggctcc
     4561 ggttcatgtc gagaaggccc gcctccgccg ccctttctgc aaacagaccg ccccgctgac
     4621 cgatgagcag gttgttcggg tcctcggctg cgaaatcgat ctgggcgtcg acgttgcctt
     4681 tggtgacgaa caagtcccag agcccgtcgt tgttgacgtc atcgaactcg gcatgccatg
     4741 ccgtcgacgg tcgcaggttg tcgccctgga agggccggtg cgcggtgacc ccggaggcga
     4801 gggcgatatc ctcgtactcg ggtcgtgatc cgtcacccac caaggtctgg agcttgttgt
     4861 ccccctgact ggtcaggaat acttcgggaa ggagatcgcc gtcgagatcc tggctggcga
     4921 tccccattcc ccagatcttg aggtgccgcc agccatcgcc ggccccatat cggcgggcac
     4981 cgtcgtcgat ctcccacagc tgctcctccc cctcggtgta gtagtgacgg tcgttggtca
     5041 tcctcaggtc gggcgacccg gagcgattcc aatcgctgaa gaggatcgag agggtgcagt
     5101 gaccgggact gagggtctcc gggccggcgt acgagtctgc atcggcggga cggaacaaga
     5161 aatggtcctc gcactcgtca cgatcaccgg agccctggat ccgcaggtag ttgccgaacg
     5221 ccagtgtcgg tagctcctca cccgactccc agaaggcgct gaaggccacg gtccactcgt
     5281 ccccaccctg gatgttccag gtctcgttcg ctcgctcgaa ctggcatcgg ccagtccccc
     5341 gcagcaccac gttctccccg cgacggagaa cgaccaggtc catgtggccg tcggagtcga
     5401 tgtcgatcgg gtaggcaccg gtgaccccga cgaggtcggt ctcactaccg ggttgggaca
     5461 cgaatgagag atcacccttt ggcaccgtct cattcacgta caacgcggcg ggccgggaac
     5521 cgccggcgag atacaggtca gggagccgat ccgagttgca gtcgaaggtg gcaacgcccc
     5581 cgcccacgaa gtactcgaac tcgccgtcgt atcggtgcac gatgcctgcg atgtccccga
     5641 cttcgacgaa gagaggcggc tcgagagcct cacctcctgt cggctcggat agccacgcgg
     5701 ctgcggccat gaccgcaccc atggtgactc cagccccgac cacgagaccc aggacgagcc
     5761 acagtgtcga gctccaagcg g
//
LOCUS       DASUTA010000346         2492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1008117, whole genome shotgun sequence.
ACCESSION   DASUTA010000346 DASUTA010000000
VERSION     DASUTA010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008117"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..369
                     /locus_tag="VFZ80_06930"
     CDS             70..369
                     /locus_tag="VFZ80_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HEX6287203.1"
                     /translation="MTTDQDSRTEEVDRVYRWARERAEMLQALYIHMLVYVVVNLGLF
                     GINYFTRGESGDWWFYWPMFLWGIGLLIHVAVTVAPFFTEEWVERKTETIAASRR"
     gene            422..2026
                     /locus_tag="VFZ80_06935"
     CDS             422..2026
                     /locus_tag="VFZ80_06935"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003995715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX6287204.1"
                     /translation="MKADLVLRGGRIFTARTREPFVNALAVSGDRIVATGSDAEELID
                     SRTRVIDLEGALATPGFIDAHVHPGTSGLDKLRCNFDDAHDAETAVAAVASYAEANPD
                     VPWILGAGWSSSWFANACPSKELLDSVVADRPVLLPNTDGHGAWANSKALEIAGIDER
                     TPDPADGRFERYADGSPQGTLHEGAFDVVMAFAPQDTVEDFVAGLLRGQDELLRYGIT
                     GWQDANVGDTMHEAYLRISGDDRLRGRVVGAMWWDRQKGLEQVDDLVERRRHGADRFR
                     PSSVKLMLDGVAENFTAAMLEPYFGENGMATANSGIDFIDPAQLREIVTRLDAEGFQC
                     HFHAIGDRAVRNALDAVEAARERNGPSTNRHHIAHIQVVHPDDVPRFAALDVVANAQP
                     LWANNDEYQTELTIPYIGPRRASWQYPFESLRREGARLAMGSDWGVSTANVMEEIHVA
                     VTRTWSSDQDPFVPAEALSPVDALNAFTAGSAYVNHAEGDSGSVAVGMLADLAVLDRD
                     PLRDGSFRDARVVTTIVGGQVVFEGP"
     gene            2026..>2492
                     /locus_tag="VFZ80_06940"
     CDS             2026..>2492
                     /locus_tag="VFZ80_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6287205.1"
                     /translation="MERAAVELVGVTKRFGDEVTAVDNLELIINDGEFFSLLGPSGCG
                     KTTTLRMIAGFEFPTEGSVRIHGSEMGLKPPNERPVNTVFQSYALFPHMTVFDNVAFG
                     LEMKKVAKSDVETRVRRVLDQVQLAQRADAKPSQLSGGMQQRVALARALVNEP"
BASE COUNT          467 a          753 c          816 g          456 t
ORIGIN      
        1 gggcagatgt ggctttcgac cctggtgctg gaggtcggtt ctgcgaagaa tcgaaccagg
       61 aggtgaaaga tgactaccga tcaagacagc cgaaccgagg aggtcgatcg cgtctaccgc
      121 tgggcccgag agcgagccga gatgctgcag gctctctaca tccacatgct ggtctacgta
      181 gtggtgaatc tggggctctt cgggatcaac tacttcacgc ggggtgaatc cggcgactgg
      241 tggttctact ggccgatgtt cttgtggggg atcgggcttc tgattcatgt cgcggtgacc
      301 gttgccccgt tcttcaccga agagtgggtg gagcgcaaga cggagaccat cgcggcgtcc
      361 aggaggtaga aagcccgacc atcgaggcgg cgtccctcac caggggccat actgccgccc
      421 gatgaaagct gatctcgtcc tcaggggtgg ccgcatcttc acggcacgaa cccgggagcc
      481 cttcgtgaac gcgctggcgg tgagtggcga tcgcatcgtg gccaccgggt ccgatgccga
      541 ggagctgatc gatagccgga cccgggtcat cgacctcgag ggagcactgg ccacacccgg
      601 tttcatcgac gcacacgttc accccgggac cagcgggctc gacaagctcc gctgcaattt
      661 cgacgacgca cacgacgcgg agacagcagt cgctgctgtg gcttcgtacg ccgaggcgaa
      721 ccccgatgtt ccctggatcc tcggtgctgg ttggtcctct tcgtggtttg ccaacgcatg
      781 tccgtcaaag gagctgctcg attcggtggt agccgaccgt cccgtgctcc ttcccaacac
      841 agacggacat ggggcttggg ccaactcgaa ggctttggag atcgcaggca tcgatgagcg
      901 gaccccagac cccgccgacg gacgtttcga acggtatgcc gacggctctc cccagggaac
      961 cctccacgag ggggcgttcg acgtggtcat ggccttcgct ccacaggaca cggtggagga
     1021 cttcgttgcc gggctcctac gaggtcaaga cgagttgctt cgatatggca tcaccggctg
     1081 gcaggacgcc aatgtcggtg acacgatgca cgaggcatac cttcggatct cgggagacga
     1141 ccgtcttcgc ggccgggtgg ttggtgccat gtggtgggat cggcagaaag gcctcgagca
     1201 ggtcgacgac ctcgtggagc ggcgccgaca tggagctgac cgattccgtc cctcgtcggt
     1261 gaagctgatg ctcgatggtg tcgcagagaa tttcaccgcg gcgatgctcg agccctattt
     1321 cggcgagaat ggcatggcca ccgcgaacag cgggatcgat ttcatcgacc cggcgcagct
     1381 acgcgagata gtgaccaggc tcgacgccga gggattccaa tgccacttcc atgcaatcgg
     1441 tgaccgggct gtgcgcaacg cgctcgacgc ggtcgaggca gcacgagaac gcaacggccc
     1501 atcgaccaac cggcatcaca tcgcccatat ccaggtggtc caccccgacg acgtgcctcg
     1561 tttcgccgca ctcgacgtgg tggccaacgc acaacccctc tgggccaaca acgacgagta
     1621 tcagacggag ctgaccatcc cctacatagg cccgagaagg gcatcctggc agtacccgtt
     1681 cgagtcgctg cgtcgggagg gtgcgcggct ggcgatgggg agcgactggg gagtgtccac
     1741 cgcaaatgtc atggaggaga tccatgtcgc agtgacccgt acctggagct ccgatcagga
     1801 ccccttcgtc ccggccgagg ccttgagccc cgtcgatgct ctgaatgcat tcaccgccgg
     1861 atcggcctac gtgaaccacg ccgagggcga ctccgggtct gtggccgtgg gtatgctcgc
     1921 cgacctcgcg gttctcgacc gggacccctt gcgggacggg tcgttccgcg acgcccgtgt
     1981 ggtgaccacc atcgtgggtg gacaagtggt ctttgagggt ccttgatgga gcgagctgcg
     2041 gttgagttgg taggagtcac caaacgtttc ggtgacgagg tcactgcggt ggacaatctg
     2101 gagctcatca tcaacgacgg tgagttcttc tccctcctcg gcccgtcggg ttgtgggaag
     2161 accacgacgc ttcgaatgat cgctgggttt gagtttccca ccgaaggcag tgtcaggatc
     2221 cacggaagcg agatggggct gaagccaccc aacgagcgac cggtgaacac agtcttccag
     2281 tcgtatgcgc tgtttccgca catgaccgtg ttcgacaacg tggcatttgg gctcgagatg
     2341 aagaaggtgg ccaagtccga cgtcgagacg cgggtccgcc gggtcctgga ccaggttcaa
     2401 ctggcgcaac gggccgacgc caagcccagc cagttgtccg gcggcatgca acagcgagtt
     2461 gccctggccc gtgccctagt gaacgagccg ga
//
LOCUS       DASUTA010000347         2591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1008299, whole genome shotgun sequence.
ACCESSION   DASUTA010000347 DASUTA010000000
VERSION     DASUTA010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008299"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFZ80_06945"
     CDS             complement(<1..284)
                     /locus_tag="VFZ80_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287206.1"
                     /translation="MLKRPLVGGIALALLLVACSSELTTGQHLLGNHTVDRNRDMPYL
                     LWLPEGMETGGEEHPMILSLHGTGPTEYSPEFVMSYGLPAVLALEEQPPA"
     gene            338..967
                     /locus_tag="VFZ80_06950"
     CDS             338..967
                     /locus_tag="VFZ80_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6287207.1"
                     /translation="MAQIRRLTDSCLHVITDDAATLFDPGFHTFLEGEIDLEGIGEVT
                     RVLISHEHRDHVHPDFVRWLVDRGEDVTVYSNQAVVDLLAEAGIDAHVDEPDGVTIED
                     VLHGMLPTGAQPPNRAFTIDGLITHPGDSREPTRTAPILALPLMAPWGSTTDAVDHAR
                     RLGPSQVVPIHDFYLNEGGRSWLRGLAKNVLGADGIEVVELDWGGSYTV"
     gene            complement(1023..1547)
                     /locus_tag="VFZ80_06955"
     CDS             complement(1023..1547)
                     /locus_tag="VFZ80_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX6287208.1"
                     /translation="MTGQSPRDIDWPTRRWLLWGLLAVLVVTAITLFFVFLGNAENEQ
                     TDNEAAPPEGAEQLVWSITLVDMTGDEVTLKGDLPLGMPTETWQNDFQVTRTVGGAFE
                     WDVEQELPATVVEIDQAAGDCEALNAILAGLVSEGDQALEAELWHLRAFAQHTINTMR
                     DQGCTIDDAALQGL"
     gene            complement(1558..2496)
                     /locus_tag="VFZ80_06960"
     CDS             complement(1558..2496)
                     /locus_tag="VFZ80_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013962423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6287209.1"
                     /translation="MATTQEQIEQQTGIQEPGSGEQHRSLRQDVWRQFKKHKGAVVGL
                     ILLTIIVLATIFGPVLWPIDPYAIETANGSAPPSAAHPLGTDNLGRDVLARLLVGGRI
                     SLSVGFAAMIFGLFLGTAVGVISGFFRKLDGPLMRITDLFLALPILPLLLVVILLFRE
                     PLQERFGEELGIFLLVVFIIGVTSWMQTARVVRGDVLSLKEEEFVLAANSIGTRRRNI
                     IVRHILPNTLSPVMVSAALGVAVAIITESALSFLGLGFPASFPTWGRLLFDANQYIQI
                     SPMRVIAPGVMISLTVLCVNYIGDGLRDALDPRLRS"
BASE COUNT          479 a          760 c          891 g          461 t
ORIGIN      
        1 aaggcggggg gttgctcttc gagtgcgagg acggccggga gaccgtagct catgacgaac
       61 tccgggctgt actccgttgg cccggtcccg tgcagcgaga ggatcatggg atgctcctca
      121 ccgccggtct ccatgccttc cgggagccag agcaggtatg gcatgtcccg attccggtcc
      181 acggtgtgat tccccaacag atgctggccg gtggtgagct cggagctaca agcgacgagg
      241 aggagtgcca gcgcgatccc accaaccagc ggccgcttca gcatgaggac agggtaggcc
      301 tcccttcccg gctctgcggg atgcgtaggt ttcgggtatg gcgcagatca gacgtttgac
      361 cgattcctgt ctccacgtaa tcaccgatga cgcggcgaca ctcttcgatc ccgggttcca
      421 cacttttctc gaaggggaga tcgacctgga aggcatcggc gaggtgaccc gggtcctgat
      481 cagccacgag caccgtgacc atgtccaccc cgacttcgtg agatggctcg tcgatcgcgg
      541 tgaggatgtg acggtctact cgaatcaggc ggtcgtggac ctgctggccg aggcgggtat
      601 cgacgctcat gtcgacgagc cggacggagt caccatcgag gacgtactcc atgggatgct
      661 gcccaccggg gcccaaccgc cgaacagggc cttcacgatc gatgggctga tcacgcatcc
      721 cggcgacagc cgagagccga ccaggacggc cccgatcctg gccttgcctc tgatggcgcc
      781 gtggggctcg accaccgacg ccgtcgacca cgccaggcgg ctcgggccta gccaggtggt
      841 acccatccac gatttctatc tcaatgaggg cggccggagc tggctgcgcg ggttggccaa
      901 gaacgtcctc ggggccgacg ggatagaagt agtcgaactc gattgggggg gctcttacac
      961 ggtctgactc gagccgtgct gtgcggtagt gtcggggtcg gtgaccccgc acagacagcg
     1021 gtctacagac cttggagagc ggcgtcgtcg atggtgcagc cctgatcacg catggtgttg
     1081 atggtgtgct gggcgaatgc gcgcagatgc cacaactcgg cctcgagagc ttggtcgcct
     1141 tcggaaacga ggccggcgag gatcgcattc aaggcttcgc agtcgccggc ggcctgatcg
     1201 atttccacga ccgttgcggg cagctcttgt tccacatccc actcgaaggc ccctccaacc
     1261 gttcgagtca cctggaagtc gttctgccat gtctcggtgg gcatgcccag tgggagatcc
     1321 cccttcagtg tgacctcgtc accggtcatg tcgacgaggg tgatcgacca caccaactgc
     1381 tcggcgccct cgggtggtgc ggcctcgtta tcggtctgct cgttctcggc gtttccgagg
     1441 aagacgaaga agagggtgat agcggtcacc acgagcactg cgagaaggcc ccacagcagc
     1501 cagcgtcggg ttggccagtc gatgtcccga ggggattgtc cggtcattcg acgcccgcta
     1561 gctgcgaagc cgggggtcga gggcgtcgcg gaggccgtcg ccgatgtagt tcacgcagag
     1621 gacggtcagc gagatcatga cgccgggagc gatcacccgc atcggggaga tctggatgta
     1681 ctggttggcg tcgaagagga gacgtcccca ggtcgggaaa ctggccggga acccgagccc
     1741 gaggaacgaa agcgccgact cggtgatgat cgccaccgcc acgccgaggg cggcagacac
     1801 catgaccggg ctcagtgtgt tggggaggat gtggcgcacg atgatgttgc gtcgccgggt
     1861 gccgatgctg ttggcggcga gtacgaactc ttcttctttc agggagagga cgtcgccacg
     1921 taccacgcgg gcggtctgca tccagctggt cacgccgatg atgaagacca cgaggaggaa
     1981 gatgcccagc tcctccccga atctctcctg gaggggttcc cggaaaagga ggatgacgac
     2041 caggaggagg ggaaggatgg gaagggcgag gaacaggtcg gtgatgcgca tcagaggccc
     2101 gtcgagcttg cggaagaacc cggagatgac tccgaccgcc gtgcccagga agagaccgaa
     2161 gatcatggcg gcgaaaccga ccgagagtga gatacgcccc ccgacgagga gtcgagcgag
     2221 gacgtcgcgg cccaggttgt cggtgcccag cggatgggcg gccgatggtg gagcagagcc
     2281 attggccgtc tcgatggcgt aggggtcgat tggccagaga acgggcccga agatggtggc
     2341 gaggacgatg atggtcagga gtatcagccc gaccacggcg cccttgtgct tcttgaactg
     2401 tcgccagacg tcctgacgca acgagcggtg ctgctcgcca cttcccggct cctgtatgcc
     2461 ggtctgctgc tcgatctgct cctgtgtggt tgccattgat tgtccgattc tgttcgatca
     2521 gtcgtatctg attcgcgggt ccagtatgcc gtaggtgacg tcggccacca gattgaagac
     2581 cacgatgagg a
//
LOCUS       DASUTA010000348         2570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_232545, whole genome shotgun sequence.
ACCESSION   DASUTA010000348 DASUTA010000000
VERSION     DASUTA010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232545"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VFZ80_06965"
     CDS             <1..329
                     /locus_tag="VFZ80_06965"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HEX6287210.1"
                     /translation="RPGFLEFNGVSTGQEVIVAVDPPSESEVNRVTTFSGYTRAFESM
                     VTVIVTQGDAVITETSTTAADSTDAWGEFEIELTLPPGPIEVFLGEANPEDGSLSGVI
                     LDLTAS"
     gene            complement(295..810)
                     /gene="rimM"
                     /locus_tag="VFZ80_06970"
     CDS             complement(295..810)
                     /gene="rimM"
                     /locus_tag="VFZ80_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013907.4"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX6287211.1"
                     /translation="MGSRGVVPPHVVGRLGRPNGLDGFIGIYVDDEDLVHLQPDSIVH
                     VAGEPHTVRAIRRGKKGHQVAFLEVTSREGAEAIRGSDVSVHERRDLSEGEYWPEDLV
                     GLEVRPGGGVVAGVSHGQAQDRLVVERAGLKFEVPFVDELVPVVDVAEGYVEVVEIEG
                     LSSPSDRGSPR"
     gene            complement(848..1105)
                     /locus_tag="VFZ80_06975"
     CDS             complement(848..1105)
                     /locus_tag="VFZ80_06975"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX6287212.1"
                     /translation="MTVAKGEIVERVVTYVVKSIVEDSDAVQVTLVEQGPDEFVAEVR
                     TAKSDMGRVIGRRGRVAKAIRAAAGAAGEEEGINAGVEFLD"
     gene            complement(1102..1500)
                     /gene="rpsP"
                     /locus_tag="VFZ80_06980"
     CDS             complement(1102..1500)
                     /gene="rpsP"
                     /locus_tag="VFZ80_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009400861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX6287213.1"
                     /translation="MAVRLRLMRMGKKKQPVYRVVVMDQRAPRDGRYIEQIGRYDARE
                     DPSIVEIDNERAADWLAKGAQPSETVEKLLNISGAMNTMKIQTGGIHVVGVDRQTAPA
                     EAAAAEATADAEEAGDAEDVPADSANTEEE"
     gene            complement(1519..>2570)
                     /gene="ffh"
                     /locus_tag="VFZ80_06985"
     CDS             complement(1519..>2570)
                     /gene="ffh"
                     /locus_tag="VFZ80_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX6287214.1"
                     /translation="TAPPLVILMVGLQGSGKTTTAGKLAKHLQAEGKRPLLVAADLQR
                     PAAIDQLETLGQRVDVPVLVDRKGKAAKLAKSALKEAEKMGRDVIIIDTAGRLQIDEA
                     LMRELASVRKAAEPDEVLLVVDAMTGQDAVNVADGFQRQIGLTGLILSKLDGDARGGA
                     AISVREVTGAPVKFAGVGEGLGDLEAFHPERMASRILGMGDVLTLIEKAETAFDQDKA
                     EKAAQRLQKGDFTLEDFLEQFEAMKSMGPLQDILAMLPGSGSLLRDVEIDDRDLKRVE
                     AVIQSMTPEERRAPKIIGGSRKRRIASGSGTTPQDVNRVLKQFAETQKMMKTFAGGRG
                     LPSLEALTGGRRRKR"
BASE COUNT          417 a          898 c          763 g          492 t
ORIGIN      
        1 tgcgacccgg gtttctcgag ttcaacgggg tgtcgacggg ccaggaagtg atcgtggcgg
       61 tggacccgcc atcggagagc gaggtgaacc gtgtcaccac cttctccgga tacacgcgcg
      121 cattcgagtc catggtcacc gtcatcgtga cccagggcga cgcggtgatc accgagacct
      181 cgacgacggc cgccgattcg accgacgcat ggggagaatt cgagatcgag ctgacgctcc
      241 ccccgggccc gatcgaggtg ttcctgggtg aggcgaaccc cgaagacggc agtctcagcg
      301 gggtgatcct cgatctgacg gcgagctgag cccttcgatc tcgacgacct cgacgtaacc
      361 ctctgcgaca tcgacgacgg gcacgagctc gtcgacgaac ggaacctcga acttcaggcc
      421 ggcccgctca accacgagcc tgtcctgcgc ctggccatgg ctcaccccgg ccaccacccc
      481 tccaccgggg cgaacttcga ggccgacgag gtcctcaggc cagtactcgc cttcggagag
      541 atcgcggcgc tcgtgaaccg acacgtcgct gcccctgatg gcctcggccc cctcacgcga
      601 cgtcacttcg aggaaggcaa cctgatggcc cttcttgccg cgccgaatcg cccgcaccgt
      661 atggggctct ccagccacgt ggacgatgct gtccggctgg agatggacca ggtcctcgtc
      721 gtcgacgtag atcccgatga acccgtcgag cccattggga cggcccaggc ggccgacaac
      781 atgagggggg acgacccccc tcgaacccac cgcggcggct tcaccgccgg atgagggctg
      841 cccgtcttca gtcgaggaat tcgactccag cgttgatccc ctcttcttcg ccggcggcgc
      901 cggcggcggc cctgatcgcc ttggcgacac ggcctcttcg tccgatgaca cggcccatgt
      961 ccgatttggc cgtgcgcacc tcggcgacga actcgtcggg gccctgctcg accagggtga
     1021 cctgaacggc gtcagagtcc tcgacgatgc tcttcaccac ataggtgacg actcgttcga
     1081 cgatctctcc tttggccaca gtcactcctc ctcggtgttg gcggaatccg ccgggacatc
     1141 ttcagcatcc ccggcttctt cggcgtcggc agtcgcctcg gcggctgcag cctcggccgg
     1201 agccgtctgc ctgtcgacac cgacgacgtg tatcccaccg gtctggatct tcatggtgtt
     1261 catcgcccct gagatgttca ggagcttctc gacggtctcg gacggctgag cacccttggc
     1321 cagccaatcg gcggcgcgct cgttgtcgat ctcgacgatg gacggatcct cccgggcgtc
     1381 ataacgcccg atttgctcga tatatcgacc atcgcgcgga gcacgctggt ccatgacgac
     1441 gacgcgatac acgggctgct tcttcttgcc catccgcatc agtctcaacc tgacagccat
     1501 caattcacct ctatcgactc atctcttgcg acgccggccg ccggttaggg cctcgagtga
     1561 cggcaagccc ctgcctccgg caaacgtctt catcatcttc tgtgtctcgg cgaactgctt
     1621 gaggacccga ttcacgtcct ggggcgttgt gcccgacccg gacgcgattc ggcgtttgcg
     1681 gctcccgccg atgatcttgg gggcccggcg ctcttcgggg gtcatcgact ggatgacggc
     1741 ctccaccctc ttcaggtcgc gatcgtctat ctcgacatca cgaaggagcg atccggaccc
     1801 gggcagcata gcaaggatgt cctggagagg ccccatcgac ttcatggcct cgaactgctc
     1861 gaggaagtcc tcgagcgtga agtcaccctt ttggagacgc tgggcggcct tctctgcctt
     1921 gtcctgatcg aaggctgtct cggccttctc gatgagagtg agcacgtccc ccatcccgag
     1981 gattcgactc gccattcgct cggggtgaaa cgcctcgaga tcgcccaatc cctcaccgac
     2041 gccggcaaac ttgaccggcg caccggtcac ctcacggacc gagatggcgg cacctcccct
     2101 ggcgtcgccg tccagtttgg agagaatgag cccggtcagg ccgatctgtc tctggaatcc
     2161 gtccgcgacg ttcactgcgt cctgaccggt catggcatcg accaccaaca gcacctcgtc
     2221 cggctcggct gccttgcgca ccgaggcgag ctctctcatc agggcttcgt cgatctggag
     2281 acgtcccgcg gtgtcgatga tgatcacgtc gcggcccatc ttctccgctt cctttagcgc
     2341 cgacttggcg agcttggctg ccttgccctt gcggtcgacg agtaccggga catccacgcg
     2401 ctggcccagg gtctccaact gatcgatggc ggcgggtcgt tgcaggtcgg cagctacgag
     2461 aagcggccgc ttcccttcgg cctgcaggtg cttggccagc ttccctgcag tcgtcgtctt
     2521 acccgacccc tgcaacccca ccatgagaat cacgagcggt ggagccgtct
//
LOCUS       DASUTA010000349         4199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1398801, whole genome shotgun sequence.
ACCESSION   DASUTA010000349 DASUTA010000000
VERSION     DASUTA010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398801"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VFZ80_06990"
     CDS             <1..729
                     /locus_tag="VFZ80_06990"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6287215.1"
                     /translation="RDADADVEAAMSATRPDVPDEADLEELLESGLTQLRRMGPINPL
                     AAQEYRELEERHTFLSEQMADVESSRAELRKVISALETEIQSRFDEAFTEVAEAYERY
                     FEVLFPGGRGRIRLVDPDDRHSGVAIDAQPLGKKVSHMTLLSGGERSLAALAFLFAIF
                     DARPSPFYVLDEVEAALDDANLRRFIRVVDEFRQRAQLIIVTHQQQTMEAADVLYGVT
                     MEPGGSSQAIRKDMRDAVVSENVA"
     gene            750..1721
                     /gene="ftsY"
                     /locus_tag="VFZ80_06995"
     CDS             750..1721
                     /gene="ftsY"
                     /locus_tag="VFZ80_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019174820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX6287216.1"
                     /translation="MDNTVTVVLVVAVVVVVGVAVGFLVIRRRPETDTVPTVPVTAPP
                     RTGLGAAIRRALGTSIDTHTWEAIEEALISADVGVAASADIVARARQEKPETADEAWL
                     AIGNSLKQELAGKHRDLRLDGNPAVVLVVGVNGTGKTTTIAKLAKRFMDEGRSVTLGG
                     ADTFRAAAAEQLETWGERLGVRVVRGQEGSDPASVAYDTIDSARARGVDVVIIDTAGR
                     LHGKKNLMSELEKIHRVSGGDAVSEVLLVLDATAGQNGLTQVSEFSEAIPVSGIVLTK
                     LDGTARGGIVVAVERTLGVPIKLVGVGERLDDLESFDPRSFVDSLIA"
     gene            1718..2113
                     /locus_tag="VFZ80_07000"
     CDS             1718..2113
                     /locus_tag="VFZ80_07000"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX6287217.1"
                     /translation="MTMDTTDLVDAARAAALGSYSPYSKFRVGAVVVGEGDEVFTGAN
                     VENAAYPASSCAEATAISAAVAHGVRKIDTVAVACIDADDVDGAYPCGRCRQIMSEFD
                     VDNVIVTAGDGAEVREHTLDELLPFRFGL"
     gene            complement(2157..2579)
                     /locus_tag="VFZ80_07005"
     CDS             complement(2157..2579)
                     /locus_tag="VFZ80_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HEX6287218.1"
                     /translation="MAGYSETPLFKKLGIVEGMTVAMLNAPNYFDFDFGDLPEGVEVL
                     RDSDPEAADIYLIFASRIAEAERGFERAVTRLPADGAIWFAWPKKTSGVDTDLTEDTL
                     RDLFLPSGMVDNKVCAIDETWSGLRFVVRKENRADWPH"
     gene            2591..3769
                     /locus_tag="VFZ80_07010"
     CDS             2591..3769
                     /locus_tag="VFZ80_07010"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX6287219.1"
                     /translation="MAVMTCVDDLLDVASDVLSARVGRTVDLLDAMQLESKSTVIRAR
                     VSSTGEDLPSTVIIKHVTDEEFDQPGHTGPPSRFLNEWSALEFLTDLDGEVAPRLIAA
                     DEGKGLTIVEDLGDLPTLENLLSAGLESSAMDGLEVAGELLGRLHAMSRDLEDEFAFI
                     QSRLGTRSPLSDSTVDQRGREDVFLAILHSLGIEPGKQFWDDLHGLESLIHDGSPFRS
                     LIHADAGPHNVLVNAADSRLIDFEFAVYGDVFSDAVGARLGFPQTMSVGQVPMRAVET
                     LEASYREAIAAGIPQAEDDALFGRHLTAACGHWALNRWRAAWRDHPSAAPSREPPAEA
                     VVRGSRSLWLVIDGFVAAVDEFGEFEALADALCRFRARATARWPELEPAPVYPAFRNL
                     "
     gene            3794..>4199
                     /locus_tag="VFZ80_07015"
     CDS             3794..>4199
                     /locus_tag="VFZ80_07015"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEX6287220.1"
                     /translation="MDNRELLEFREGKDEFFRTNHHSPLGHGDRSEFTGLDYFEPNSD
                     LIFTVPIENGDGSEVRFPTTDDAEKVYTNAGTVSFEVQGQEVELTLYETGHPGFFLPF
                     RDATSGKTTYGAGRYLDLEPNDDGTLTIDFNYA"
BASE COUNT          773 a         1266 c         1383 g          777 t
ORIGIN      
        1 cgcgatgccg atgccgatgt ggaagcggcg atgtcggcga ccaggcccga cgtcccggac
       61 gaggccgatc tcgaagagct gctcgaatca gggctcacac agctgagacg aatgggcccg
      121 atcaacccgt tggctgccca ggagtaccgc gagctcgaag agcggcacac gttcctctcc
      181 gagcagatgg ccgacgtcga gtcttcccgg gccgagctgc gcaaggtgat ctcggcactc
      241 gagaccgaga tccagagccg cttcgatgag gctttcaccg aggtcgccga agcctacgag
      301 cggtacttcg aagtcctgtt ccccggtggc cgggggcgaa tccgcctcgt cgaccccgac
      361 gatcggcatt caggtgtggc catcgacgcc caaccgctcg gcaagaaggt ctcccacatg
      421 actcttctct ccggtggcga gcgcagtctg gccgccctcg ccttcttgtt tgcgatcttc
      481 gacgcccgac ccagcccgtt ctatgtcctc gacgaggtgg aagcggctct cgacgacgcc
      541 aacctgcgga gattcatccg tgtcgtcgac gagttccgcc agcgagccca gttgatcatc
      601 gtcacccatc aacagcagac gatggaggcg gctgacgtgc tctacggggt caccatggag
      661 ccggggggct cgtcgcaggc gatccgcaag gacatgaggg acgcggtcgt ctccgagaac
      721 gtcgcctagc tctctagatt cagcgaccaa tggacaacac agtcacagtc gtattggtcg
      781 tggcggtcgt ggtcgtggtc ggggtcgccg tcgggttctt ggtcatcagg cgaaggccgg
      841 agacagacac ggtgccgacc gtccctgtca ccgcaccccc gaggacgggt ctcggcgcgg
      901 cgatacgacg ggcgctcgga acctcgatcg acacgcacac atgggaggcg atcgaggagg
      961 cgctgatatc ggccgatgtc ggagtggctg catcagctga catcgtggcg cgagcccgcc
     1021 aggagaagcc tgagaccgca gacgaggcat ggctggcgat aggcaattca ctgaagcagg
     1081 agctcgccgg caagcacagg gacctgagat tggatggaaa ccctgcggtg gtgctcgtcg
     1141 tcggggtcaa cggcacaggc aagacgacga caatcgccaa gctggccaag cgattcatgg
     1201 atgaggggcg gagtgtcacg ctcggcggtg ccgacacgtt tcgagccgcg gccgcagagc
     1261 agttggagac gtggggcgag cgactcgggg tcagagttgt tcgtggccag gaaggaagcg
     1321 acccggcgtc ggtcgcctac gacaccatcg actcggcgcg tgctcgtgga gtcgacgtgg
     1381 tgatcatcga cacagccggg cgtctccacg gcaagaagaa cctcatgtcg gagctggaga
     1441 agatccatcg agtctcgggt ggcgacgcag tcagcgaagt ccttctcgtc ctcgacgcga
     1501 ctgccggcca gaacggtctc acccaggtga gtgagttctc cgaggcaatc ccggtctcag
     1561 ggatcgtcct cacgaagctg gacgggacgg cgcgcggcgg catcgtggtc gccgtcgaga
     1621 gaaccctcgg cgtgcctatc aagttggttg gtgtcggaga gcgcctcgac gacctcgagt
     1681 cgttcgatcc gcgtagtttc gtggattcat tgatcgcatg acgatggaca ccaccgatct
     1741 cgtcgatgct gctcgtgccg cggcgctggg ctcgtactcg ccatattcga agttccgcgt
     1801 gggtgcggtg gtagtcggtg agggtgacga ggtcttcacg ggggcaaacg tcgagaacgc
     1861 ggcgtatcca gcgtcttcgt gcgccgaagc cactgcgata agcgctgcgg tcgcccacgg
     1921 ggtgcgcaag atcgacacgg tcgcggttgc ctgcatcgat gccgatgacg tcgatggtgc
     1981 ctacccatgt gggcgatgcc ggcagatcat gtccgagttc gatgtggaca acgtgatcgt
     2041 caccgccggc gacggcgccg aggtcaggga gcacactctc gacgagctgc tcccgttccg
     2101 tttcggtttg tgagcgtgtg cagatactgg cctgacaggc cagtatctgc acacgctcag
     2161 tgcggccagt cggcccgatt ctccttacgc accacgaaac ggaggcccga ccaggtctcg
     2221 tcgatggcac acaccttgtt gtccaccatc ccggaaggca ggaagaggtc gcgcaaggtg
     2281 tcctcggtca ggtcggtgtc cacacccgac gtcttcttcg gccaggcgaa ccagatggct
     2341 ccgtcagcgg gtaggcgagt cacggctcgc tcgaacccac gctcggcttc ggcgatccgc
     2401 gacgcaaaga tcaggtagat gtccgccgcc tccggatcgg agtcacgcag gacctcgaca
     2461 ccctccggca ggtcaccgaa gtcgaagtcg aagtagttcg gagcattgag catggcgacc
     2521 gtcatgcctt ccacgatccc aagcttcttg aagagcggtg tctcgctgta gccggccatg
     2581 gtcgacgatt atggcggtca tgacgtgtgt cgacgatctc ctcgacgtcg caagcgatgt
     2641 cctgagtgcc agggtcggca gaacggtcga tctgttggat gcaatgcagt tggagagcaa
     2701 gagcacggtg atccgggctc gcgtctcttc aaccggcgag gacttgccgt ccaccgtgat
     2761 catcaagcac gttaccgacg aggagttcga ccagcccggc cacaccgggc cgccgtcacg
     2821 attcctcaac gagtggagcg ctctcgagtt cctgaccgac ctcgatgggg aggtcgcacc
     2881 gagattgatt gcggccgacg aaggcaaggg gctcacgatc gtggaggact tgggcgacct
     2941 gcccacgctg gagaacctgt tgtccgccgg actcgagagc tccgccatgg atgggctcga
     3001 ggttgccggc gaattgctcg gtcgcctcca tgcgatgtcg agagatctcg aggatgagtt
     3061 cgctttcatc cagtcgcggt tgggcactcg gtcaccgctg tccgattcca cggtcgacca
     3121 gcgggggcgt gaggacgtgt ttctcgcgat tctccacagt ctgggtatcg agccagggaa
     3181 gcaattctgg gacgacctcc acgggctgga gtcactgatt cacgacggat cacctttccg
     3241 ttctctgatc catgccgatg cggggccgca caacgtgttg gtgaacgctg cggactcacg
     3301 tctgatcgat ttcgagttcg ccgtctacgg cgacgtgttc agcgatgcgg tcggtgctcg
     3361 actcggcttc ccccagacca tgagtgtggg gcaagtgccg atgagggccg tcgagaccct
     3421 ggaggcttcc tatcgggagg ctatcgccgc gggcattccg caagccgaag acgatgctct
     3481 gttcggacgt catttgaccg cggcgtgcgg ccattgggca ctcaatcggt ggcgggctgc
     3541 gtggcgagac cacccgagtg ccgccccgag ccgtgagcct cccgccgaag ccgtggtcag
     3601 gggttcgaga agcctttggt tggtgatcga cggcttcgtt gctgccgtcg acgagtttgg
     3661 tgagttcgaa gccctcgccg atgccctgtg ccggttcaga gccagggcga ctgcgcgttg
     3721 gccggagctc gagccggcac cggtctaccc ggcattccgg aatctgtgaa ggcggggccg
     3781 ggttgacatg ctcatggaca atcgagagct gctggagttt cgtgagggca aggacgagtt
     3841 cttccgaacc aaccatcact cgccgctggg ccatggcgat cgctccgaat tcacggggtt
     3901 ggattacttc gagcccaact ccgatctgat cttcaccgtg ccgatagaga atggtgacgg
     3961 gtcggaggtg cgatttccca ccaccgatga cgccgagaag gtgtacacga acgccggtac
     4021 ggtgagcttc gaggtccaag gacaggaagt cgagctgact ctctacgaga cggggcaccc
     4081 ggggttcttt ctccccttca gggacgcaac atctggcaag accacctatg gagccggtcg
     4141 ctatctcgat ctggaaccga acgacgacgg gactctcaca atcgacttca actatgcgt
//
LOCUS       DASUTA010000350         4954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_103023, whole genome shotgun sequence.
ACCESSION   DASUTA010000350 DASUTA010000000
VERSION     DASUTA010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103023"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFZ80_07020"
     CDS             complement(<1..525)
                     /locus_tag="VFZ80_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287221.1"
                     /translation="MILVAVPAVALAAVTLLSFLGRWVWWLDVLANFRAQYVVALAIL
                     GLIVAMSKWRKTGYAILGVALVNLVVVLPLYIGSPAEARIDADTIRVMSFNLLSDNEQ
                     YSDVIDYIRTIDPDLVLLHEASRPWEVAIDSAELEYEVVRARSEDLIFGTLVLVRGEG
                     ITAVSHGFAAGAARA"
     gene            complement(711..2132)
                     /locus_tag="VFZ80_07025"
     CDS             complement(711..2132)
                     /locus_tag="VFZ80_07025"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX6287222.1"
                     /translation="MIPERLRYLSRPELPPAQLAEIFAGAGEQLYLVGGSVRDAILGR
                     DLEALDFDFATSARPDRVRALVDPWAEVVYEVGAQFGTIAARRSGRTVEITTFRKEVY
                     RDDSRKPHVTYSDDIETDLGRRDFTINAIALSLPDLEAVDPHDGLGDIARTSLRTPLD
                     PEVSFGDDPLRMLRLFRFQAMLEFAPEESALAAVREMAARLEIISAERIRDELSKLLV
                     APAPGEALEGIVESGLAGYFLPELPRLAMEQDPDHHHKDVLAHTFAVVDKTSPDLVLR
                     LAALFHDVGKPDTREFGPGGVSFHHHEVVGARMARRRLKQLRYPKEVVDDVSQLVYLH
                     LRPHTLKLGWTDSAVRRYVRDAGDLLEPLNELVRCDVTTANARRERVIQARLDELEAR
                     IEHLSAQEELARLRAPIDGNEVMSYLGIDPGPKVGEIMDILLEKRIDDGPYSRREAYG
                     VARTWAIERGLADPGEPPPDEEE"
     gene            complement(2192..3271)
                     /locus_tag="VFZ80_07030"
     CDS             complement(2192..3271)
                     /locus_tag="VFZ80_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX6287223.1"
                     /translation="MENNVDVLLKQAVTHGASDIHLKVGSAPIVRIEGELRRLDGYEP
                     LRPADTRAYAEAMFTEKAARDFDETGSTDFAYGRHELGRFRATAFRQRGSVSMVLRRV
                     VPGSRSFMELGLPRAMERIAGFESGLVLVTGPSGSGKTTTMASLIDWINSNKGRAIIT
                     IEDPIEVLHPDKRSVVVQREVGVDTRDIAGAIRAAMRHDTDVILVSEISAPDVAQAAI
                     DAAETGHLVISSMRTSDPADTVNRLISMFTQQQQGIIRGQLASQLKAVISQILVDTGQ
                     TGQVLGCEILTSNERSQEWILNGAEASHLIEVMKEGGFHGMQTFDQALLEHVLEGGVD
                     IESILPYVRNTHEVKAKALAAGVAV"
     gene            complement(3343..4350)
                     /locus_tag="VFZ80_07035"
     CDS             complement(3343..4350)
                     /locus_tag="VFZ80_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX6287224.1"
                     /translation="MEVVVASHPSALLHDTGRRHPERPARVEAVRAGIEESGLEVHSI
                     EAPAIERSELALVHDSSYIEMIHRFCAEGGGALDMDTFVSYESWEAALTAAGGVRALV
                     EELESRSNATGFALSRPPGHHALAAKAMGFCLFNNVAVTAAYLRSRGERVAILDWDVH
                     HGNGTQAMTADDPGTLYVSIHQDHFYPFEGSVGDIDHGDAKGTVVNIPVPAATAGDVY
                     RRAWSDLVIPVVSQFEPSWVFLSAGFDAHAADHLAHLRLESSDYGWMAAKLCEIHPVN
                     RTVVVLEGGYDLDALRDSARATVRGLAGETPTEGGRVSPPQSETAIETAATAIARHWS
                     I"
     gene            4369..>4954
                     /locus_tag="VFZ80_07040"
     CDS             4369..>4954
                     /locus_tag="VFZ80_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6287225.1"
                     /translation="MASLLRVDGSWPGPITLSSGFFRARARPWNDFCTDPMVRLDRGG
                     ADFLRLVSTHFAEEGVGSVYSPALYPNATGVWRRSGFQTHAELAVMERSLNRAPDKPT
                     HEVSSPVQPDWKSVSRLDDAAFDGFWRMSRLALEEAYETARTSALLETHVDGRLAGYA
                     IVGNQWGISYLHRIAVHPDHAGQGRGASLLTAAAG"
BASE COUNT          854 a         1584 c         1635 g          881 t
ORIGIN      
        1 ggctcgagcc gcaccggcgg cgaagccgtg agagaccgcg gtgatcccct cacctcgcac
       61 cagcaccagg gtcccgaata tgaggtcctc cgaacgggcc cgcaccacct cgtactcgag
      121 ctcggcggag tcgatcgcga cctcccacgg tcgacttgcc tcgtgcagca gcactagatc
      181 gggatcgatc gtcctgatgt agtcgatgac gtcggagtac tgctcgttgt ccgacagcaa
      241 gttgaaagac atcaccctga tggtgtctgc gtcgatgcga gcttccgccg gtgagccgat
      301 gtagaggggg aggacgacga ccaggttgac caaggccact cccaggatcg catagccggt
      361 cttgcgccac ttggacatgg ccacgatcag gcccaggatc gccagtgcca ccacgtactg
      421 cgcccggaaa ttggcgagaa catcgagcca ccagacccat cgaccgagaa atgagagcaa
      481 tgtcactgcc gccagggcga cggcgggaac tgcaaccagg atcacgcggt caggctaggt
      541 ccggccggat ctctacgatc ggtcggagtc cttcgacccc tgcgagcctg atcgaccgat
      601 ctcgggcctg gggggcccgt ggtgagttgt accggactcg gccagctcgt cgaggtcggg
      661 ccaggatcca accagctccc gcacccgatc cccagtgatc gaacccgggt ctactcctcc
      721 tcgtcaggcg gaggctcgcc cggatcggcg agacctcgtt cgatggccca tgtccgggcc
      781 actccgtagg cctctcgtcg actgtagggg ccgtcgtcga tccgcttctc caagaggatg
      841 tccatgatct caccgacctt cggccccgga tcgatcccga gatacgacat cacttcgttt
      901 ccatcgatcg gggctcggag cctcgccagc tcttcttgcg cggaaagatg ctcgatgcga
      961 gcctcgagct cgtcgaggcg ggcctggatg actctctctc ttcgcgcgtt ggcggtggtc
     1021 acatcacaac gcaccagctc gttgagcggc tcgaggagat cccccgcgtc ccgcacgtaa
     1081 cggcggacgg cggagtcggt ccagcccaac ttgagcgtgt gaggccgaag atggaggtag
     1141 acgagttgag agacatcgtc gaccacctct ttcgggtacc gcagctgctt caggcggcgg
     1201 cgggccatcc gcgccccgac tacctcgtgg tgatggaagc tgacaccacc gggcccgaac
     1261 tcgcgggtgt ctggcttgcc aacgtcgtgg aagagggcgg cgagacggag caccaggtcg
     1321 gggctggtct tgtcgactac cgcgaacgta tgtgccagga cgtccttgtg gtggtgatcc
     1381 ggatcctgct ccatggccaa gcgggggagc tccgggagga aatacccggc gagaccggac
     1441 tcgacgatgc cctccagggc ctccccgggt gcaggggcga cgagcaactt ggagagctcg
     1501 tcccgaatcc gttctgcact gatgatctcg aggcgagcgg ccatctcacg caccgctgcc
     1561 agagcggact cctcgggagc gaattcgagc atcgcctgga accggaacag gcgcagcatg
     1621 cgaagcgggt cgtcgccgaa ggagacctca gggtcgagtg gagtgcgcag agaggtgcgc
     1681 gcaatgtcgc cgagaccgtc atgagggtcg accgcctcga ggtcggggag ggagagcgcg
     1741 atggcgttga tcgtgaagtc ccgccggccg aggtcggtct cgatgtcgtc ggagtaggtg
     1801 acatgaggct ttcgcgaatc gtcccggtac acctccttgc ggaaggtggt tatctcgacg
     1861 gtgcgcccgg agcgtcgggc ggcgatggtc ccgaactggg ctccaacctc gtacaccact
     1921 tccgcccagg gatcgaccag cgctctcacc cggtcgggcc gtgcgctggt ggcgaagtcg
     1981 aagtcgagcg cctcgaggtc gcggccgagg atggcgtcac ggaccgaacc ccccaccagg
     2041 tagagctgct caccggcccc ggcgaagatc tccgccaatt gggcaggcgg gagctcaggt
     2101 ctgctcaggt atcggagacg ctccgggatc acggttggca ggttatcggc ggcctcgctc
     2161 ggccccaggg gaataggaga ggaccgattc gtcagacggc gacgccggcg gccagtgcct
     2221 tggccttgac ctcgtgggtg ttgcgaacgt atggcaggat cgactcgatg tcgacgccgc
     2281 cttcgaggac atgctcgaga agggcctggt cgaatgtctg catgccatgg aagccgccct
     2341 ctttcatgac ctcgatcagg tgggaggcct ccgccccatt caagatccac tcctgggagc
     2401 gctcgttgct ggtgaggatc tcacagccca ggacctggcc ggtctgaccg gtgtcgacga
     2461 ggatctggct gatcacagcc ttcaactggc tggccaattg cccgcgaatg atcccttgct
     2521 gctgctgggt gaacatcgag atcaggcggt tgaccgtgtc ggcgggatcg ctggtgcgca
     2581 tcgacgagat gacgaggtga ccggtctcgg ctgcgtcgat cgccgcctga gcgacgtccg
     2641 gtgcgctgat ctctgagacc aggatcacat cggtgtcatg tcgcatcgcg gcgcgtatcg
     2701 cgccggcgat atcccgggtg tcgacaccga cctcccgctg gaccaccacg gagcgcttgt
     2761 cggggtgcag cacctctatc gggtcttcga tggtgatgat cgcccggcct ttgttggagt
     2821 tgatccaatc gatgagcgag gccatcgtcg tggtcttgcc agacccggac ggcccggtca
     2881 ccaggacgag tccgctctcg aatccggcaa tgcgctccat cgctctggga agccccagct
     2941 ccatgaagga ccgcgatccg gggacgactc gacggagaac catcgagacc gagccacgct
     3001 gtctgaacgc ggtggcacga aaacggccga gctcgtgcct gccgtaagcg aagtcggtcg
     3061 acccggtctc gtcgaagtcg cgggccgcct tctcggtgaa catcgcctcg gcataggcgc
     3121 gggtgtcggc gggtcggagc ggctcatagc catctagccg gcgcaactcg ccctcgatgc
     3181 gcacgatcgg cgccgagccc accttgaggt ggatgtcgga tgcgccatgt gtgactgcct
     3241 gcttcaacag gacgtcgacg ttgttctcca tcggctcaga tacctcgcct agctccgagc
     3301 atcgttatcg ggcgaagggg gctgcgggtt tagggaatcg gctcagatcg accaatggcg
     3361 agcgatcgcg gtggccgcag tctctattgc cgtctccgac tgcggcgggg agacccggcc
     3421 cccctccgtc ggcgtctcac cggctagccc tctcacagtc gcccgggccg agtcgcgcaa
     3481 cgcgtcgaga tcgtatccgc cctccaagac gacgaccgtc cgattgaccg gatggatctc
     3541 gcagagcttt gcggccatcc agccgtagtc ggaggactcg agacggaggt gggcgagatg
     3601 gtcggcggca tgcgcgtcga acccggcgct caggaacacc cacgagggct cgaactggga
     3661 gacgaccggg atgacgaggt cagaccaggc cctgcggtag acgtcgccgg cagtcgctgc
     3721 gggtaccggg atgttcacca ccgtgccctt ggcgtctccg tggtcgatgt cgccgacgct
     3781 gccctcgaac gggtagaagt gatcctggtg gatcgagacg taaagcgtcc ctgggtcgtc
     3841 cgctgtcatc gcctgggtgc cgttgccgtg atgcacgtcc cagtcgagga tggcgacccg
     3901 ttctccccgg gatcggaggt atgcagccgt cactgcgacg ttgttgaaca ggcagaaccc
     3961 catcgccttg gcagccaggg cgtggtgacc gggagggcgg gacagagcga atcctgttgc
     4021 gttgctcctc gactcgagct cctcgaccag ggcacgaact ccgccggcgg cggtcagggc
     4081 ggcttcccag gattcgtacg agacaaacgt gtccatgtcc agcgcacctc caccttccgc
     4141 acagaaacgg tggatcatct cgatgtagga ggaatcgtga accagggcca gctcgctgcg
     4201 ctcgatcgca ggggcctcga tcgagtggac ctccagccca ctctcctcta tcccggcccg
     4261 gaccgcctct actcgcgccg gtcgctcggg gtgtcgtcga ccggtgtcat ggagtagcgc
     4321 cgaagggtgg ctggcaacta cgacttccat gttcatactc tcccacaagt ggcgtctctt
     4381 ctccgcgtcg acgggtcatg gccgggaccc atcaccctca gttccgggtt cttccgagca
     4441 agggcgagac cttggaacga cttctgcacc gacccgatgg tccggctcga tcgtggagga
     4501 gcggacttcc ttcgcttggt ctcaacgcat ttcgccgagg agggagtcgg ctctgtctac
     4561 tcgcctgctc tatacccgaa tgccaccggc gtgtggcggc gaagcgggtt ccagacccat
     4621 gccgagttgg cagtgatgga gcgctccctg aaccgggccc cggacaaacc cacgcatgag
     4681 gtctcgtccc cggtccagcc cgactggaag tcggtttcgc gactcgacga cgctgccttc
     4741 gatggtttct ggcggatgag tcgcctcgca ctcgaggagg cgtacgagac cgcgaggacg
     4801 tcggctctgc tcgagaccca cgtcgacggg cgcctcgccg gctatgcgat tgtgggcaat
     4861 caatggggta tctcatacct gcaccggata gcggtgcatc ccgaccatgc cggtcaggga
     4921 cggggtgcct ctttgctgac cgccgcggcg ggct
//
LOCUS       DASUTA010000351         2552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_103914, whole genome shotgun sequence.
ACCESSION   DASUTA010000351 DASUTA010000000
VERSION     DASUTA010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103914"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VFZ80_07045"
     CDS             <1..455
                     /locus_tag="VFZ80_07045"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEX6287226.1"
                     /translation="SPLGHGDRSEFTGLDYFEPNSALVFTVPIENGDGSEVRFPTTDD
                     AEKVYTNAGTVSFEVQGQEVELTLYETGHPGFFLPFRDATSGKTTYGAGRYLDLEPND
                     DGTVTIDFNYAYNPSCVYGDGFSCPLPPVNNWLQVPIEAGEKDYPPET"
     gene            complement(437..973)
                     /locus_tag="VFZ80_07050"
     CDS             complement(437..973)
                     /locus_tag="VFZ80_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6287227.1"
                     /translation="MPRHILTGAPGTGKTTVLELLATRYVTVTEPAREVIAEHMAATG
                     ETTLDHRPESFVEKLIERSLRDYESASDTEITLFDRGLPDCAAYAAIFGLDEAPTLEI
                     ASKYRYESPVFMTRPWRAIYTTDEMRRATYQQVEAFHEILRQVYLRLDYEIAEVPQIS
                     PGGRAAFIEERLGQVSGG"
     gene            complement(978..1478)
                     /locus_tag="VFZ80_07055"
     CDS             complement(978..1478)
                     /locus_tag="VFZ80_07055"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEX6287228.1"
                     /translation="MPRRYLLFVLDGEAETVQRLRRQWDPVMADRIDAHLTLVYPQEF
                     DDEELLLERAAAAASHTAPFEVRPGTVERSNLGGVWARVLDDSGAWAELRSSILTPPF
                     TPYPVTPHITIVHPRTSDRSREALASLNGTRIWQPLRVEELFYTETGKAGTRVLDRFP
                     LTACLE"
     gene            1664..>2552
                     /locus_tag="VFZ80_07060"
     CDS             1664..>2552
                     /locus_tag="VFZ80_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEX6287229.1"
                     /translation="MQRSGIQFGPEHDACGVGFIAARDRVASFHMTRLAVECLQRLDH
                     RGAKAADGTGDGAGLLTQIPHKLIARELAARGKHIEPEHVGVVMCFLPPDDPAPSRYL
                     VSEALEAEGIRLMHWRSVPMDPSVLGEHAREVLPLIEQAVVTSDLTGDEFERALFLAR
                     KRMERGAPPGFSIPSASSRTVVYKGLFTASRIAEFYWDLRDPDFETAFALFHQRFSTN
                     TFPSWDNAQPFRALAHNGEINTIQSNRSWMVARERGATPGVWGDRLADVFPFLQPDLS
                     DSASLDNAFELLLQSGRSLP"
BASE COUNT          442 a          822 c          807 g          481 t
ORIGIN      
        1 actcgccgtt gggccatggc gatcgctccg aattcacggg gttggattac ttcgagccca
       61 actccgctct ggtattcacg gtgccgatag agaatggtga cgggtcggag gtgcggtttc
      121 ccaccaccga tgacgccgag aaggtgtaca cgaacgccgg tacggtgagc ttcgaggtcc
      181 aagggcagga agtcgagctg actctctacg agacggggca ccccgggttc tttctcccct
      241 tcagggacgc aacgtctggc aagaccacct atggagccgg tcgctatctc gatctggagc
      301 cgaacgacga cgggactgtc actatcgact tcaactatgc gtacaacccg tcctgtgtat
      361 acggagatgg cttctcgtgc ccattgccac cggtgaacaa ttggctgcag gtgccaatcg
      421 aggcggggga gaaggactac ccgccggaga cttgaccgag gcgttcctcg atgaacgctg
      481 ctctcccacc tggtgagatc tgtggcacct cggcgatctc gtagtcgagc ctgagataga
      541 cctgtctgag gatttcgtga aaggcctcga cctgttggta cgtcgccctg cgcatctcgt
      601 cggtcgtgta gatcgctcgc cacggtcgag tcatgaacac cggggactcg taccgatact
      661 tcgaagcgat ctcgagcgtg ggtgcttcat cgaggccgaa gatggcggcg taggcggcgc
      721 agtcggggag accacgatcg aagagggtga tctcggtgtc ggaagccgac tcgtagtccc
      781 gtagcgatcg ctcgatcagc ttctcgacga acgactccgg acgatggtcc agggtggtct
      841 cgccggttgc tgccatgtgc tcggcgatga cctcgcgagc cggctcggtg actgtcacgt
      901 atcgggtcgc caacagctcg aggaccgtgg tcttccctgt gcccggggca ccggtcagga
      961 tgtggcgcgg catcagctca ttccaggcat gcggtcagcg ggaaccggtc gaggactctc
     1021 gtgcctgcct tgccagtctc cgtgtagaag agttcctcga cacgaagtgg ctgccagatt
     1081 cttgtcccat tcagcgatgc cagggcttcc cggctgcggt ccgaggtccg cgggtggacg
     1141 atggttatgt gcggggtcac cggatagggc gtgaatggtg gcgtgagaat cgagctcctc
     1201 aactctgccc aggcgccgga gtcgtccaga acgcgggccc atacccctcc caagttggat
     1261 cgttcgacgg ttcccggtct cacctcgaag ggagcggtgt gcgatgcggc ggcggctgcc
     1321 ctctccagca gcagctcctc gtcgtcgaac tcttgggggt agaccagcgt gaggtgggca
     1381 tcgattcgat ccgccatgac gggatcccac tgacgccgta gcctctgcac agtctcagcc
     1441 tcgccgtcga gcacgaagag cagataacgg cggggcatcg gacccattgt ggccggagag
     1501 cccctcctgc gcgcaacgct ggcgatgacg gcgagccagc ccaatctccg caacccgggg
     1561 ttgcgcaacg aagcccgtgg ctccgggtag gagaccgcac tgccacctga ctcgggagat
     1621 cggggcacct gaccctaaga tccgcagttc cctcgaggtg gatatgcaga ggtccggtat
     1681 ccaattcggc cccgagcacg acgcctgtgg cgtcgggttc atcgctgccc gagaccgggt
     1741 tgcttcgttc cacatgactc gtctcgccgt ggagtgcctc caacgtctgg accaccgtgg
     1801 cgccaaggca gccgacggaa ccggagacgg cgccgggctc ctcacccaga tccctcacaa
     1861 gctgatcgcc cgtgagctgg cggcccgggg taagcacata gagccggagc acgtcggtgt
     1921 ggtcatgtgc tttctcccac ccgacgatcc ggcgccctcg cgctacctcg tctcagaggc
     1981 tctcgaggcc gaaggaatcc ggctcatgca ttggcgctcg gtccccatgg acccatccgt
     2041 cctcggtgag catgcccgcg aagtgctgcc gctgatcgaa caggcggtgg tcacctcgga
     2101 tctgaccggg gacgaattcg aaagggcctt gttcttggcc cgaaagcgga tggagcgcgg
     2161 cgctcccccc ggcttctcca tcccatcggc atccagccgc acggtggtct acaagggtct
     2221 cttcaccgcg agccggatcg ccgagttcta ctgggacttg cgggatcccg acttcgagac
     2281 ggccttcgcc ctcttccacc agcggttttc cacgaacacc ttcccgagct gggacaatgc
     2341 ccagcccttc cgcgcccttg cccacaacgg ggagatcaac accatccagt ccaatcgctc
     2401 gtggatggtg gctcgggagc gcggcgccac gcccggggtc tggggagacc gactggccga
     2461 tgtcttcccg ttcctccagc ccgacctgtc cgactcggca agcctcgaca acgccttcga
     2521 gttgctgctg cagagcggcc ggtctctacc cc
//
LOCUS       DASUTA010000352         2027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_364435, whole genome shotgun sequence.
ACCESSION   DASUTA010000352 DASUTA010000000
VERSION     DASUTA010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364435"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VFZ80_07065"
     CDS             complement(<1..433)
                     /locus_tag="VFZ80_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020020229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX6287230.1"
                     /translation="MRADNYVIPTVFEQTSRGERYFDIYSRLLKDRIIFLGTPIDDTI
                     ANLIMAQLLHLESDEPDKDIFLYINSPGGSITSLFAIYDTMQYIRPEVNTVCMGMAAS
                     AAAVILAGGAPGKRYALPHARIMLHQPHGGAQGQASDIEIQA"
     gene            complement(430..1806)
                     /gene="tig"
                     /locus_tag="VFZ80_07070"
     CDS             complement(430..1806)
                     /gene="tig"
                     /locus_tag="VFZ80_07070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX6287231.1"
                     /translation="MPTTVTETGAFERRVRFQLTDEQINEAKKGAARKLASEVKIHGF
                     RPGKAPLQIVEATVGADRVRQEAIDDLLNPVLNEVLDAEEITPAINPELESIDDVDGG
                     VEVEVKVTLWPTIQLPKYKDRKVEVSNPAVTDEELAEQTRRMLEQFSTVEEVERAARL
                     GDFVSIDLEASKDGQDVEEAKAADILYEVGSGLLIEGMDDEIKGAEAGDRVSFTGRLP
                     DGFGERGGEEVVFTVTVHEVKERILPELTDEWVDENTEFDTVDELTAALSLRLAHIKL
                     LAVSREYSEKAMSTLRDQIEVDLPEGLILSEMDRHLHNFLHRLEEAELTLDDYLETSG
                     VSQATFVADLRSQADLSLRNRLVLEAVIEAEDLKIEPEELSVALQSLAAQSEDPVAYL
                     RAIRESGQELAVASDILRNRALDAILSNTNPVDEDGNPVDLSLNVNEVEAEIIDDEHV
                     VMAEEEEE"
     gene            complement(1884..1960)
                     /locus_tag="VFZ80_07075"
     tRNA            complement(1884..1960)
                     /locus_tag="VFZ80_07075"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1924..1926),aa:His,seq:gtg)
BASE COUNT          355 a          640 c          620 g          412 t
ORIGIN      
        1 tggcctggat ctcgatgtcg ctggcctgcc cctgagcacc accgtgtggc tggtggagca
       61 tgattcgggc gtggggcagg gcataacgct tgccaggggc gccacccgcg agaatgaccg
      121 cggcggcaga ggcagccata cccatgcaca ccgtgttcac ctcgggtctg atgtactgca
      181 tggtgtcgta gatcgcgaaa agtgacgtga tcgagccacc aggcgagttg atgtacaaga
      241 agatgtcctt gtccggctca tccgactcga ggtggagcaa ctgagccatg atgaggttgg
      301 cgatcgtgtc atcgatggga gtccccagga agatgatgcg gtccttgagc agccggctgt
      361 agatgtcgaa gtagcgctca cccctgctcg tctgctcgaa gacggtgggg atcacatagt
      421 tgtcggctct cattcctctt cctcctcggc catcacgacg tgctcatcat cgatgatctc
      481 ggcctcgact tcgttgacgt tcaaactcag gtcgaccggg ttgccgtcct cgtcaaccgg
      541 gttggtgtta gacaagatgg cgtccagcgc tctgtttctg agaatatcac tggccacggc
      601 caactcttga ccgctctccc ggatcgctcg cagataggcc acggggtctt cggactgagc
      661 cgccaacgac tggagcgcca cggacaactc ctcgggctcg atcttgaggt cctccgcctc
      721 gatgacggcc tcgaggacga gacggttccg gagcgacaaa tcggcctgtg aacgcaggtc
      781 ggccacgaag gtcgcctgac tgacccccga ggtctcgagg taatcgtcca gggtgagctc
      841 cgcttcttcg agacgatgca agaagttgtg aaggtgtcgg tccatttcgg aaaggatcag
      901 accctcgggc agatcgactt cgatctggtc acggagcgtg ctcatcgcct tctccgagta
      961 ctcgcgggag acggccaaga gcttgatgtg ggcgagacgc aacgaaaggg ccgcggtcag
     1021 ttcgtcgacc gtgtcgaact cggtgttctc gtctacccat tcgtcggtca gctcgggaag
     1081 gattcgctcc ttgacctcgt ggacggtgac ggtgaacacc acttcctcac cgccgcgctc
     1141 cccgaatccg tcgggcaacc tacctgtgaa cgagacccgg tcacccgcct ccgctccctt
     1201 gatctcgtca tccatgccct cgatcagcag accagacccg acctcataga ggatgtccgc
     1261 ggccttggcc tcctcgacat cctgcccgtc cttggaagct tcgagatcga tcgagacgaa
     1321 gtcccccaac cgggcagcgc gctccacctc ctcgaccgtg gagaattgct cgagcatcct
     1381 cctcgtctgc tcggcgagct cctcgtccgt gacggccgga ttggacacct cgaccttgcg
     1441 gtccttgtac ttggggagct ggatggtggg ccagagtgtc accttcacct ccacctccac
     1501 tcccccgtcg acgtcgtcga tcgactcgag ctcggggttg atggcgggcg tgatctcctc
     1561 ggcgtcgagc acctcgttca acaccgggtt gagcagatca tcgatggctt cctgacggac
     1621 ccgatcggcg ccaacggtgg cctcaacgat ctggagaggg gccttgcccg ggcggaaccc
     1681 atggatcttg acctcgctgg ccagcttgcg ggcagccccc ttcttggcct cgttgatctg
     1741 ctcgtcagtg agctggaagc gcacgcggcg ctcgaaggcg ccggtttcgg tgacggtggt
     1801 gggcatgtgt ttcctgtgga tagtcgtgtt attcggcaac gggtaccgaa ttcgggccgc
     1861 ccggcattgc cgggcggccc ctgtggggtg accgacggga cttgaacccg cgacttcctg
     1921 gaccacaacc aggcgctcta ccaggctgag ctacggccac catgtggcga atgggtctcc
     1981 agcgcccccg ggaggactcg aacccccaac ctgcggatta gaagtcc
//
LOCUS       DASUTA010000353         2196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_879260, whole genome shotgun sequence.
ACCESSION   DASUTA010000353 DASUTA010000000
VERSION     DASUTA010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879260"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VFZ80_07080"
     CDS             complement(<1..549)
                     /locus_tag="VFZ80_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287232.1"
                     /translation="MRRKAVVWMLVALTISACAQSTLSPSTTEEGSTTSSPTPTSTLP
                     PVVDCPGAGEFGEGSGIASVEGESSDATRLGRVSWERSELCESFVFEFETSEGAPATS
                     VPDITVEHLPSLQVIRISMNVTSTVITDQLVETDMVQRLYVVRSLDDGMFVDLHLNTP
                     AAARARVQSSPGVLTVDLRPGFL"
     gene            621..1784
                     /locus_tag="VFZ80_07085"
     CDS             621..1784
                     /locus_tag="VFZ80_07085"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX6287233.1"
                     /translation="MSTAVIVATARSPIGRAYKGSLIDVRADDLAGFAINALMERVPD
                     LDRASVDDVMLGCGLTHHEQGFNLARPASLLGGMPDTVPATTVNRFCASSLQTIRMAY
                     HAIERGEGDTYIAAGTESVSRTYGKAMDSDDLNPRFTDPERDDYINNVYIPMGNTAEN
                     VARKYGISRERQDEFGKRSQDRAVEAQKNGFFDWEITPIDLPDGRTVRDDDGPRPNTT
                     LEKLAELPPVFDPNGTVTAGNSCPLNDGSAAVLVMAEEKAAALGLQPLARIIASSVAG
                     LEPEYMGMGPVYAVRRVLDQSGMTLDDVDVIELNEAFAAQVLACVDELGIDMDKLNPH
                     GGAIALGHPFGMTGARIMGTLLNDLRSLDGSIGLETMCVGGGQGMAMLVERLS"
     gene            1863..>2196
                     /locus_tag="VFZ80_07090"
     CDS             1863..>2196
                     /locus_tag="VFZ80_07090"
                     /inference="COORDINATES: protein motif:HMM:NF013874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX6287234.1"
                     /translation="MRISVVTMFPGYFDGPLQVSIVGRAIEDGELDVELIDLREHGIG
                     KHRQLDDTPFGGGPGMVMMVEPLASALGPLEHTHRVLFTPTGSPLTQDTLDRWASQPH
                     LTLVCGRFE"
BASE COUNT          424 a          688 c          686 g          398 t
ORIGIN      
        1 gagaaacccg ggtcgcagat cgaccgtgag cacacctggg gatgattgga ctcgtgcccg
       61 ggccgcagca ggtgtgttga gatggagatc gacgaacatg ccgtcgtcga gcgaccggac
      121 gacatagaga cgctggacca tgtcggtttc gaccagctga tcggttatca ccgtggaggt
      181 gacgttcatc gagatccgga tgacttgcag tgacgggaga tgctcgaccg tgatgtccgg
      241 cacggacgtg gccggggctc cctccgaggt ctcgaactcg aatacgaagc tctcacacag
      301 ctccgatcgc tcccatgaga cccgtccgag ccgagtggca tccgagctct ccccttcaac
      361 gctcgcgatg ccgctgccct ctccaaactc gcccgcaccg gggcagtcga ctaccggtgg
      421 aagtgtcgac gtcggggtgg gcgacgatgt agtggaccct tcttcggtgg tcgaggggga
      481 gagcgtgctc tgagcacagg cgctgatggt caacgcaacc agcatccaga cgaccgcttt
      541 cctcctcatg gcctcagagt atgcacggtg actagagttt gagtgcacgc tcaagatcgt
      601 ccccgtgaat cgaggtccac gtgtccactg cagtcatcgt cgccacagct cgttccccga
      661 tcggccgggc ctacaagggc tccttgatcg acgtcagagc cgacgacctc gccgggttcg
      721 ccatcaacgc tctgatggag agagttcctg atctggaccg ggcctcggtc gatgacgtca
      781 tgctgggttg tgggctgact catcacgaac agggtttcaa cctcgcccgg cccgcatcac
      841 tgctcggggg catgcccgac acggtgccgg cgacgacggt gaatcgattc tgcgcctcgt
      901 ccctccagac catccgcatg gcctaccacg ccatcgaacg aggagaggga gacacctaca
      961 tcgcggcggg gaccgaatcg gtgagcagaa cctatggcaa ggccatggat tcggatgacc
     1021 tgaaccctcg tttcaccgat cctgagcgag acgactacat caacaacgtc tacatcccca
     1081 tgggcaacac tgcggagaac gtggcaagga agtatgggat ttcgcgggaa cggcaggacg
     1141 agttcggcaa gcgctcccag gaccgggctg tggaagcaca gaagaacgga ttcttcgact
     1201 gggagatcac cccgatagac cttcccgatg gtcggaccgt tcgcgacgat gacggccctc
     1261 gccccaacac gacattggag aagctggcgg aactcccgcc ggtcttcgac cccaacggca
     1321 ccgtaacggc cggaaactcc tgccctctca acgatggctc ggcggcagtc ctcgtgatgg
     1381 ctgaggaaaa ggccgcggcg ctggggctgc agccattggc ccgcatcatc gcctcctccg
     1441 tcgccggtct cgagccggag tacatgggga tgggcccggt gtacgccgtg cgcagggtcc
     1501 tcgatcagtc cgggatgaca ctggacgatg ttgacgtcat cgaattgaac gaggctttcg
     1561 cggctcaggt ccttgcctgt gtcgacgagc tgggcatcga catggacaag ctcaatcccc
     1621 acggaggcgc gatcgcactc ggccacccct tcggcatgac aggtgcccgc atcatgggca
     1681 cactcctcaa cgacctgcgc tctctcgacg gctcgattgg tctcgagacg atgtgtgtcg
     1741 gcggcggcca ggggatggcg atgctcgtgg agcggctcag ctgaagcgtg tgcagatagc
     1801 ggggccagag cacagtatct gcacacgctc gagccgcctg cccgtcgttc tacccttcca
     1861 ccgtgagaat ctccgtggtc accatgttcc ccggctactt cgacgggccg ttgcaggtct
     1921 cgatcgtagg aagggccatc gaggacggtg agctggatgt cgagttgatc gacctgcgag
     1981 agcatgggat cggcaagcac agacagctcg atgacacacc ttttggaggg ggaccgggaa
     2041 tggtgatgat ggtcgagcct ctggcgtcgg ctctcggtcc gctcgagcac acgcatcggg
     2101 tcctcttcac cccgacaggt tcgccattga ctcaggacac cctcgaccga tgggcctccc
     2161 agccacacct cacgctcgtc tgcggccgat tcgaag
//
LOCUS       DASUTA010000354         2781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_879479, whole genome shotgun sequence.
ACCESSION   DASUTA010000354 DASUTA010000000
VERSION     DASUTA010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879479"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /gene="rsmH"
                     /locus_tag="VFZ80_07095"
     CDS             complement(<1..686)
                     /gene="rsmH"
                     /locus_tag="VFZ80_07095"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX6287235.1"
                     /translation="MNQGQSKEQAGFHRPVLVDEVVELMAPVLPGVVVDATFGGGGHT
                     RRMLDEFGDEVNVIGIDRDPDAVAQAVGIRATVVEGNFGDLLDVVGRVTDEPISGVLF
                     DFGVSSHQLDEPERGFSYRQEGPLDMRMGPDAGLTADEVINEWPAEDIARIIRDYGEE
                     PLARRIAGAIVAARPIDSTAMLSTVIANAMPAARRRAGHPARRTFQAIRMVVNAELDA
                     VSSGVGQAVDL"
     gene            complement(787..1206)
                     /locus_tag="VFZ80_07100"
     CDS             complement(787..1206)
                     /locus_tag="VFZ80_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX6287236.1"
                     /translation="MFLGEYFHSLDGKGRVVMPSGFRRRLEDGCVITKGQDGQLIIFA
                     ADDFESKAAEVMARPQDKEGRRFSRTIFAGADLQTPDKSGRVLVKPDLREFAGLETGT
                     EVAVLGLFDHIELWEKDRHLQEKASGDESYIDQEEDE"
     gene            complement(1569..2072)
                     /locus_tag="VFZ80_07105"
     CDS             complement(1569..2072)
                     /locus_tag="VFZ80_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX6287237.1"
                     /translation="MARALVLNASYEPLSVVSERRAVVLVLNRKASMVVSRDEMWSSE
                     STIVELPSVVRLNRYVKVPYRRAVPVTRRAVFGRDGHACQYCGGPAESLDHVTPRSQG
                     GDHTWENVVACCRSCNVRKGARTPDESGLRLRSQPGPPRRFAWIYASTGYQVDPSWHP
                     YLLGKSA"
     gene            complement(2139..2699)
                     /locus_tag="VFZ80_07110"
     CDS             complement(2139..2699)
                     /locus_tag="VFZ80_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287238.1"
                     /translation="MSSSLASRLGDIPGVASVVVDLDGFGRGIDVRLEPGADEALVME
                     RLRALLAAYGVRSERRPRREVGRAARHVSDLGVDVTITPTDGGARIEVATGLVRSSRV
                     VSADPMAVAQGLADVWCQVIGRVPIEIVSVEVGEDGSLAVVGNDGGEQVRSSRAAESG
                     WIDALTGAIGDVIGATGRPNLELAVS"
BASE COUNT          487 a          938 c          868 g          488 t
ORIGIN      
        1 aggtcgactg cctggcccac acccgatgag acggcgtcga gctcggcgtt gaccaccatc
       61 ctgatggcct ggaaggtgcg tcgggcagga tgacctgccc gacgccgggc cgcgggcatc
      121 gcatttgcga tgacggtgga cagcatggca gtggaatcga tggggcgggc ggcgacgatc
      181 gctccggcga tgcggcgggc gaggggctcc tccccgtagt cacggatgat gcgggcgatg
      241 tcctcggcgg gccactcgtt gatgacctcg tcggccgtga gcccggcatc tgggcccatc
      301 cgcatgtcga gcgggccttc ctggcggtag gagaatccac gctccggctc gtcgagctga
      361 tgtgaggaga ccccgaagtc gaagagaacc cctgagatcg gctcgtccgt gacacggccc
      421 acgacatcga gaaggtcgcc gaagtttcct tcgacgactg tcgcccggat tccgacggcc
      481 tgggccaccg catcggggtc gcggtcgatg cctatcacgt tcacctcgtc tccaaactca
      541 tccagcatgc gtctcgtgtg gcccccgccc ccgaaggtgg cgtcgaccac cacaccgggg
      601 agcaccggag ccatcagctc gacgacttcg tccaccagga ccggccggtg aaagccggcc
      661 tgctccttgc tctggccctg gttcattgcg agccctccgg gcgctggtac caactagagg
      721 cgggcggagt caggggccat ccaattggtt ccggagggct cgcaatgagc ccagacctgt
      781 tcgtcgctat tcgtcctcct cctgatcgat gtagctctca tcgccggagg ccttctcctg
      841 taggtgacgg tccttctccc agagctcgat gtgatcgaac agcccgagga ctgcgacctc
      901 ggttccggtc tcgagacctg caaactctct gagatcgggc ttcaccagga cacgacccga
      961 cttgtccggg gtctggaggt cggcgccggc gaagatggtc cgggagaaac ggcgtccctc
     1021 tttgtcctgg gggcgggcca tcacctcggc ggccttcgac tcgaagtcgt cggcagcgaa
     1081 gatgatgagt tgaccgtcct gtcccttggt gatgacacaa ccgtcttcga gacgacggcg
     1141 aaaaccggag ggcatgacca cccgtccttt gccgtcgagg ctgtggaagt actcaccgag
     1201 gaacacttct gctcttttcc gttcgtcgtc actgtcctgt gcccccaatt cgcggggtgt
     1261 gcgccccctt gctgagcgct tgcgccacta tgacccactt ccccccactt gtcaaccctt
     1321 tcccccacaa tgcttatggc gaccgacttc gacggcccac ctcctcgtga cgaacggcct
     1381 cgaggagcag ggggtcaccc tcacgaatac ctcccaaggg gtgttctgcc gtcgaacaga
     1441 cgccggtcgg tcgcattgac acggcgaccg acttcggaat ggtcttcagg tcagggtgac
     1501 gcggacgaga agccgcgacg agtccgggtc ctgatggcag gtatcaagta ctgcgaggga
     1561 agcgagccct aagcgctctt gccgagcagg tagggatgcc aggaggggtc gacctggtag
     1621 ccggtcgacg cgtagatcca ggcgaagcgg cggggtgggc cgggctggct gcggagtcgg
     1681 agaccgctct cgtcgggagt ccgcgcaccc ttgcgcacgt tgcaggagcg acagcaagcc
     1741 acgacgttct cccaggtgtg gtcgccgcct tgagatcgcg gcgtgacgtg atcgagcgat
     1801 tcagccggtc cgccgcagta ctgacaagcg tggccgtcgc gaccgaacac ggcccggcgg
     1861 gtgaccggga ccgcacgtcg atacgggacc ttgacatagc ggttgagccg aaccaccgat
     1921 ggcagctcga cgatggtcga ctccgacgac cacatctcgt ctcgactcac caccatgctc
     1981 gccttgcggt tgaggacgag gacgacggcc cgccgttcgc taactaccga tagcggctcg
     2041 taactcgcgt tcaagaccaa tgctcgggcc atctgttcag tcctaccgaa tcgaagcgta
     2101 ataggtggct cgacttgctc gggcgatcgc gcctcaggtc agctgactgc cagctccaga
     2161 ttcggtctgc ccgtggcgcc gatcacatcg ccgatggcac cggtcagtgc gtcgatccaa
     2221 ccgctctcgg cggcacgtga gctccgcacc tgctccccgc cgtcgttgcc tacaacggcc
     2281 agggacccgt cttctccaac ttcgacggag acgatctcga tcgggacccg gccgatgacc
     2341 tgacaccaga cgtccgccag tccctgagcc acggccatcg ggtcagcaga caccacccgc
     2401 gacgagcgga ccaggccagt ggccacctcg atgcgggcgc cgccgtcggt aggtgtgatg
     2461 gtgacatcga ccccgaggtc ggagacatgc cgggcggccc tccccacctc tctgcgcggc
     2521 cttcgctccg aacgcacgcc atacgctgcg agcagagcgc gcagtctctc cataacgagc
     2581 gcctcgtccg ctccgggctc gagacgaacg tcgattcctc ggccaaaacc atcgagatcg
     2641 acgacgaccg aggcaacacc gggtatgtcg ccgagtcgac tcgccaaact cgagctcatg
     2701 ccctccccca cttccgaatg actctcatct ccctacgcca ggagagatag tcaagcagat
     2761 cttgcgcgct cgcggtggcc a
//
LOCUS       DASUTA010000355         2229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_622965, whole genome shotgun sequence.
ACCESSION   DASUTA010000355 DASUTA010000000
VERSION     DASUTA010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622965"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VFZ80_07115"
     CDS             <1..296
                     /locus_tag="VFZ80_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX6287239.1"
                     /translation="PSFGDIFSNNCVNVGLLTVVLPPDAVDRIHDLAQNPQETITIDL
                     EHQVVEVDDHVFEFDFDDEPKQRLLKGLDPIGLTLEEDAAITAYEARRPAWMA"
     gene            complement(321..1373)
                     /locus_tag="VFZ80_07120"
     CDS             complement(321..1373)
                     /locus_tag="VFZ80_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX6287240.1"
                     /translation="MRPPPRPVRRLIIAPGIVLGVVAAAVTLPVIVVGAAFISRYVPG
                     KWRVLRIVWFLFLYLLVEAAALTMLFSLWVISGFGLRIGAPGFIDAHYGLMAWMLRRI
                     VASAKFTFKLNIVREGSERSSAGARPGRRPIMVLSRHAGPGDSILLMDALANSFKRHP
                     RIVLKEFLQWDPMIDVMLNRLPSAFVASGKAGRNELLEKIEEMASTMDDNDAFVIFPE
                     GANYTEKRAKRSIEKLREMGRPDLAERAEELKNTLPPRSTGVKTALAVAPENTDVFFV
                     GHAGLETFVTPGDIWRGIPVGTNVAARVWRVPAEEIPPPELQEDWLFDVWAEIDDWIT
                     DRLIDTEEPFDDPHAY"
     gene            complement(1370..2221)
                     /locus_tag="VFZ80_07125"
     CDS             complement(1370..2221)
                     /locus_tag="VFZ80_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX6287241.1"
                     /translation="MVYRGRALTTAFVLGGGGRWGAVEVGMIQALHEAGVVPDLILGT
                     SIGAFNGAVIADYPGQEGVDRLTGFWEEVTGVDLFQTSFMDRAMRVATMKPSLHDSDE
                     LRSLIEHAIHPDTKIEDLHTRFQCVAASIEKSREHWFDSGSLVDAVLASSAVPALFPP
                     VEIGGEHFYDGGLVDSVPLGRAVDMGADVIYVLQVGRIESPLRPPERLYEAALISFEI
                     ARRHRFAMTMERLPDGIGVHLLPTGSPVSWDDRRQLRWKDTGGIEERMQVAYESTSAY
                     LQEQGIG"
BASE COUNT          433 a          737 c          658 g          401 t
ORIGIN      
        1 ccccttcgtt cggcgacatc ttctccaaca actgcgtgaa cgtcggtctg ctgaccgtgg
       61 tgctgcctcc cgacgcggtc gaccgcatcc acgacctggc ccagaatccc caggagacca
      121 tcaccatcga cctggagcat caggtcgtgg aggtcgacga ccacgtcttc gagttcgact
      181 tcgatgacga gcccaagcag aggctgttga aaggtctcga cccgatcggg ctcaccctgg
      241 aagaggacgc cgccatcacc gcctatgaag cgagacgccc cgcctggatg gcttgaaccc
      301 cgggttcgtg gcagggagac tcagtaggcg tgggggtcgt cgaagggctc ctcggtgtcg
      361 atgagacgat cggtgatcca gtcatcgatc tcggcccaga cgtcgaacag ccaatcctcc
      421 tggagctccg gtggggggat ctcctcggcc gggacccgcc agacccgggc ggccacattg
      481 gtgcccaccg ggatccctcg ccagatgtca ccgggtgtga cgaaggtctc caacccggcg
      541 tgcccgacga agaagacatc ggtgttctcc ggagccacgg ccaacgccgt cttcaccccc
      601 gtcgaccgtg gcggaagcgt gttcttgagc tcctcagctc tctcagccag gtcaggtcgt
      661 cccatctccc tcagcttctc gatcgagcgc ttggctcgct tctcggtgta gttggcacct
      721 tcggggaaga tcacgaaggc gtcgttgtcg tccatcgtcg aggccatctc ctcgatcttc
      781 tcgagcagct cgttacgtcc cgccttgcca ctggccacga aggccgatgg aagacggttc
      841 agcatcacgt cgatcatcgg gtcccattgc aggaactcct tgaggacgat cctcggatgg
      901 cgtttgaatg aattggccaa tgcgtccatg agcaatatcg agtcgcccgg cccggcatgc
      961 cgactcaaca ccatgattgg tcgccggcct ggacgggcgc cggccgacga gcgctcactc
     1021 ccctcacgga cgatgttgag cttgaaggtg aacttcgctg aggcgacgat gcgtcgcagc
     1081 atccaggcca tgagcccata gtgggcgtcg atgaaccccg gtgcgccaat tctgagccca
     1141 aagcccgaga tcacccagag agagaacagc atcgtgagtg cggctgcctc taccagcagg
     1201 tagaggaaga ggaaccagac gatgcgcagg acacgccact tccccggcac gtacctggag
     1261 atgaatgcag ctcccacgac gatcaccggc agagtcaccg cggcggcgac cactccgaga
     1321 acgatgcccg gggcgatgat cagtcttcga accggccgcg gaggcggtct caaccgatcc
     1381 cttgctcttg caggtatgcc gaggtcgact cgtatgccac ctgcatccgc tcctcgatcc
     1441 ctccggtgtc cttccacctg agctgacgtc ggtcgtccca tgacaccgga ctgcccgtgg
     1501 gcaggagatg cacgccgata ccgtcaggca acctctccat ggtcatggcg aacctatgcc
     1561 gcctggctat ctcaaatgag atgagagcgg cctcatagag ccgctccggt ggccgcaaag
     1621 gggattcgat ccttccaacc tgcaggacat agatcacgtc ggcccccatg tccacggctc
     1681 tgcccaaagg caccgaatcg accaggcctc cgtcgtagaa gtgctcgcca cctatctcga
     1741 ccggtggaaa cagcgcaggg acggccgaag aggcgagaac cgcgtcgacc aatgagccgg
     1801 aatcgaacca gtgctctctc gacttctcta tcgaggcagc tacacactgg aaccgtgtgt
     1861 gcagatcctc gatcttggtg tcggggtgga tggcgtgctc gatgagactg cggagctcgt
     1921 ccgagtcatg cagcgacggc ttcatcgtgg ccacccgcat cgcccgatcc atgaagctgg
     1981 tctgaaagag gtcgacccct gtgacctctt cccagaaccc ggtgagacga tccacgccct
     2041 cctggccggg gtagtcggct atcacagcgc cgttgaaggc accgatcgag gtcccgagga
     2101 tgaggtcggg gacgactccg gcctcgtgca gagcctggat catgcccacc tcgactgcgc
     2161 cccatcgccc gccaccgccg aggacgaagg ctgtcgtcaa agctcttcct cgatatacca
     2221 ctctgtgaa
//
LOCUS       DASUTA010000356         3780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1400557, whole genome shotgun sequence.
ACCESSION   DASUTA010000356 DASUTA010000000
VERSION     DASUTA010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400557"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1787)
                     /locus_tag="VFZ80_07130"
     CDS             complement(132..1787)
                     /locus_tag="VFZ80_07130"
                     /inference="COORDINATES: protein motif:HMM:NF005907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HEX6287242.1"
                     /translation="MAQPQPIAFQPSFDDLSTPLFDVTFCVLDLETTGGSPRDCEITE
                     IGAVKYQHGELLGTFQTLVDPGLPIPPSITILTGITQAMVIDAPRIAAVLSLFLEFIG
                     DSVIVGHNVRFDMSFLNAAASRLGYDKLANKTCDTAALARRLVRSEVRNLRLQSLAAH
                     FRSPTTPNHRALEDARATAHVFHALLERAGSLGVTNLDDLLQLPTARGSAHYSKIGLT
                     DGLPRRPGVYTFRDRNGKAIYVGKASNLRARVRQYFYGDTRRTISNMMKELDSIEHQV
                     CDSVLEAEVTELRLIHAHRPRYNRKSRPPKSSHFVKLTAERYPRLSVVRTVKEDGLAY
                     LGPFRSKKSADMVVNAIWDAFPIRRCRSRPGSRTGKCAPAQMGLARCPCDGDLDEGQY
                     ARVVSGLIEAVTARPQRLLTNLEDRMGRLAREQRYEEAARARDRHDALVRALQRRWEW
                     VALSSAGWLELEHADGTVAVVDHGVLVETRPPGTSPRLRPAKRPAMSPSPPVAPSVES
                     AEEAHLIWRWIESAPVRVVECTGLFAYPIERLERLTALYDLAA"
     gene            complement(1853..2638)
                     /locus_tag="VFZ80_07135"
     CDS             complement(1853..2638)
                     /locus_tag="VFZ80_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX6287243.1"
                     /translation="MDHGITSPTNDRIKWLVRLRDRKHRDAEGVFVVEGSRLFQRALA
                     AGLEPIVTFVSDPDIEVTIGERVGVAPDILDKVSYRSRSQGLISVFEQFDVPLDRLDP
                     GPVPLLLIVENVEKPGNLGAMCRTASAAGADAIIAVGDTVDRWNPNAVRSSTGAVFTL
                     PVVTTCWDDVAPWLADRGVEIVAATPRADEKLWDIDLTGPVAIAIGAEDEGLSARAEA
                     TADHLVAIPQSSTSVDSLNASVAAGVGLFEAVRQRDVGAETAG"
     gene            2673..2900
                     /locus_tag="VFZ80_07140"
     CDS             2673..2900
                     /locus_tag="VFZ80_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX6287244.1"
                     /translation="MDLVVLGTYNSLTEANLVKARLESEGIEAIVQSDDVGSMTPTLN
                     TVRGVQVLVRSGDRAEAMESLERMLGAGDEA"
     gene            complement(2905..3501)
                     /locus_tag="VFZ80_07145"
     CDS             complement(2905..3501)
                     /locus_tag="VFZ80_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX6287245.1"
                     /translation="MEFKDVLARRRMVRNYTESPVDHNKLDRIAAAALRAPSAGNSQA
                     IGVVVVTDPSLRSEIAGLADEPAYVEMGFDPWISKAPAHLVISVSEKAYRDRYSEPDK
                     LGEDGSEIDWPVPYWWVDAGASMMAVLLATVDEGLAAGFLGVHSVPALSALLEIPEHF
                     HPIGVVTVGHPAPDRRSGSLDRPKKTPGDVVFNDRWGA"
BASE COUNT          704 a         1164 c         1245 g          667 t
ORIGIN      
        1 acctcgggca gatcctcgag ctcgctgccc gatccgtacg agcccgagca gccggcgacc
       61 agagcgggaa cgatcgcgag gagaaggagt cttcgtcgca tcgatcgggg ccaacacggc
      121 gcgccggcga cttatgccgc caggtcatat agagcggtca gacgctccaa tcgttcaatc
      181 ggataggcaa agagcccggt gcactccacc acccgaaccg gggcactctc gatccagcgc
      241 cagatcagat gtgcttcctc tgcgctctcc accgacggtg cgacaggagg agaagggctc
      301 atcgctggtc ttttcgcggg acgaagacgt ggagaggtgc ctggaggccg ggtctcgacg
      361 agcaccccgt ggtcgacgac ggccaccgtc ccgtccgcat gctccagctc gagccagccg
      421 gcggaggaca gtgccaccca ctcccatctc ctctgtagtg cccgaaccag ggcgtcgtgc
      481 cgatcccgcg cccgggccgc ctcctcatat ctctgttctc gggcgagtct gcccattcga
      541 tcttcgagat tggtgagcag gcgctgtggg cgtgcagtca cagcctcgat cagacccgac
      601 accaccctgg cgtactggcc ctcgtccaga tcgccatcgc aagggcatcg agccaggccc
      661 atctgagccg gtgcgcactt cccggtgcgg gaaccaggtc tcgatcggca ccggcgaatc
      721 gggaacgcgt cccaaatggc gttgacgacc atgtctgcgg acttcttgga gcggaaaggc
      781 cccaggtagg cgagaccgtc ctctttgacg gttcgcacca cagagagacg tgggtagcgc
      841 tcagcggtga gcttgacgaa atgggagctc ttcggggggc gtgacttccg gttgtatcga
      901 ggacgatggg cgtggatcag acgcaattcg gtcacttcag cttcgaggac cgagtcgcac
      961 acctggtgct ctatcgagtc gagttccttc atcatgttgg agatggtgcg acgtgtgtcc
     1021 ccgtagaagt actgccgcac ccgcgcccgg agattggacg ccttcccgac gtagatcgcc
     1081 ttgccgtttc ggtccctgaa tgtgtacacc ccgggacggc ggggaagacc atcggtcagc
     1141 ccgatcttgg agtagtgagc actgccgcgc gccgtcggca gttgaagcag gtcgtcgaga
     1201 ttggtgaccc cgagtgagcc cgcgcgttcg agaagggcat gaaagacgtg agcggtggca
     1261 cgtgcgtctt ccagggcccg atgattcggt gtcgtcgggc ttcggaagtg agcagccagg
     1321 ctctgcaagc gcagattgcg cacctctgag cgaaccaagc ggcgggcgag agcggccgtg
     1381 tcgcaggtct tgttggcaag cttgtcgtac ccgagtctcg atgccgctgc attcagaaac
     1441 gacatgtcga atcgaacgtt gtgacccacg atcaccgaat cgccgatgaa ttcgaggaac
     1501 aacgacaaga cagcggcgat cctcggtgcg tcgatcacca tcgcctgagt gatccccgtg
     1561 aggatggtga tcgaaggcgg gatgggcaga cccgggtcga ccagggtctg gaaagtgccg
     1621 agcaactccc cgtgttgata tttgaccgct ccgatctcgg tgatctcgca atccctgggc
     1681 gaccctccag tcgtctccag gtcgagtacg caaaaggtga cgtcgaagag cggggtcgac
     1741 aggtcgtcga aactgggctg gaaagcgatc ggctggggct gggccaaggc tcggatgagg
     1801 ctaactcgaa catatgttcg agtcaaccac gctgatacgc tcatcttcgc gctcagccgg
     1861 ccgtctcagc tcctacgtct ctctgcctga cagcctcgaa gagacccaca cctgccgcca
     1921 ccgacgcgtt cagcgagtcc acactggtcg agctctgtgg tatcgccacc agatgatcgg
     1981 cggtcgcctc ggcccgcgct gacaaccctt cgtcctcggc gccgatcgcg attgccaccg
     2041 ggccggtgag gtcgatatcc cagagcttct cgtcggcgcg tggggtagcc gccacgatct
     2101 ccacaccgcg gtcggccagc cacggtgcga cgtcgtccca acatgtcgtc acgaccggca
     2161 gggtgaacac cgcacctgtg gacgatcgga cggcgttggg gttccaccgg tcgacagtgt
     2221 caccaaccgc gatgatcgcg tcggctccag cagctgaggc ggtgcgacac atggcgccga
     2281 gattgcccgg cttctcgacg ttctcaacga tcaggagcag cggaacaggg cccgggtcga
     2341 gtcgatcaag ggggacatcg aactgctcga acaccgagat caggccctgg gaccgggacc
     2401 ggtaggagac cttgtcgagg atgtcggggg cgacgccgac acgttcgccg atggtcactt
     2461 cgatgtcagg gtcggagacg aaggtgacga tgggctcgag gccggcggcc agagcgcgct
     2521 ggaagagacg ggatccttcg accacgaaca ccccttctgc gtcccggtgc ttgcggtctc
     2581 gaagacggac cagccatttg atccggtcgt tggtcggaga agtgatcccg tggtccaccg
     2641 gcgcagtatc ccaccgacct actctccggc tgatggatct ggtagtgctc ggcacctaca
     2701 actcactcac cgaggccaac ctggtgaagg cgcgtctcga gagcgagggg atcgaggcga
     2761 tcgtgcagtc cgatgatgtg ggctccatga cgcctaccct caacacggtc cgtggagtgc
     2821 aagtgctggt ccgatcgggc gatcgggccg aggcgatgga gtcgctggag cggatgctcg
     2881 gcgccgggga cgaggcgtga ccggctaagc gccccagcga tcgttgaata ccacgtcgcc
     2941 gggtgtcttc ttgggccgat ccaggcttcc cgaccgacgg tccggggcgg ggtgacccac
     3001 ggtgacgact ccgatcggat ggaagtgctc cggtatctcc agcagtgccg agagggcggg
     3061 gaccgagtgg actcccaaga agccggcagc gaggccctcg tccacggtgg cgaggaggac
     3121 ggccatcatc gaggcgccgg cgtccaccca ccagtacgga accggccagt cgatctccga
     3181 accgtcctca ccgagcttgt cgggctccga gtagcggtcc ctgtacgcct tctcactgac
     3241 cgagatgacc aggtgggcag gcgctttgga gatccagggg tcgaagccca tctcgacgta
     3301 ggccggttca tcggcgagac cggcgatctc tgatcgcaat gacggatccg tcaccaccac
     3361 gaccccgatg gcctggctgt tccctgccga gggagcgcgg agcgcggctg cggcgattcg
     3421 gtcgagtttg ttgtgatcga ccgggctttc cgtgtagtta cggaccatgc gtcgtcgtgc
     3481 caggacatct ttgaactcca tggcgcccga ttctgtcacc tcgggcggtc aagctggtca
     3541 cggaactctc ttacgaatcg aggagttgag acctacagtg acacccgtgg ttgtgacacc
     3601 aggcagaaac cacacggtgg gatcggcgtc gtctgcgggc gactcgttgg gatcaacgtc
     3661 gtgaaggtcg tatggaccag aggcaagggt tgcctcgagt tggccgaggc cctaggtggg
     3721 ggagagacga gccgaatcca aggtgaccta tccgaggagt gcatatccga gcgcgccgat
//
LOCUS       DASUTA010000357         2715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1789793, whole genome shotgun sequence.
ACCESSION   DASUTA010000357 DASUTA010000000
VERSION     DASUTA010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789793"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..457
                     /locus_tag="VFZ80_07150"
     CDS             185..457
                     /locus_tag="VFZ80_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287246.1"
                     /translation="MVDRIAEGWKTADLDPVDVALCEYTEKLTRAPAAVGPEDIEALR
                     RAGLDDQGISSATQVIAYFNYINRIAEGLGVPMETWLNDDGTLEDA"
     gene            469..1944
                     /locus_tag="VFZ80_07155"
     CDS             469..1944
                     /locus_tag="VFZ80_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HEX6287247.1"
                     /translation="MAKDKKKKKKPKPKHPPELKDRKVSKQFEGTFGGKKVSYTTTAA
                     TQVFESNDEKVSFFYVSYTRDEADPAERPVLFCFNGGPGSSTVWLNLGLYGPKRMEFD
                     DEGFKTGMQGRLVDNEHSILDVTDVVCIDAMGTGFSRTAEKDSETKFHHFKKDIEAFS
                     EFIVHYLNRNGRWASPKYLSGESYGTLRGAGIARELFATHNVEFNGIVFISMILNYQT
                     SGIDADNMLIHPGNDLPFALYLPTYAATARYHGRLSEKYQKMALPDFLDEVEEFALGE
                     YWSALALGDRIDDGRKKEVAERLSAYTGLDAGFIDHYDLRIQIHRVCKELLRDQHRTV
                     GRIDSRYVGIDRVKAGNVIETDPSLDATGGVAASALNHYLRDEIGFDTHDIYRIMGPN
                     VWKNWDYEDFKGRYVDTSENFRELLSRSRGTKVLVANGYYDLATPHFATEYTFSHMGL
                     DPEVRENVALTYYEAGHMMYVHKPSLEQLATDLREFVTTTS"
     gene            2055..>2715
                     /locus_tag="VFZ80_07160"
     CDS             2055..>2715
                     /locus_tag="VFZ80_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6287248.1"
                     /translation="MILKEFRQVGRDRRTLAMLIVMPVVLLLVFGYAASFDVEEIPTI
                     VVGENAELAADALPEILVVEESDAAATREEAIAGLVAGRAHVAVVADGSDPVVLIDGS
                     ELFMARAAVQVATSMSSRVPGLTHEVLFNPELDTSAALVPGLAGLIIVFVGTLVTSMS
                     VVREREAGTLEQLAVMPFKPKDVFVGKIAPYFLIAAFDIVMVLIIGTLIFDVPFNGSV
                     WP"
BASE COUNT          534 a          881 c          832 g          468 t
ORIGIN      
        1 gagccagctt cccgaggtga tgcgtgattc gatgcgcatc taccagtcga tcatgttcgg
       61 gccgtcgcca ttgaccaggg cgcaacggga gatgatggcc gtcgtcacct cgcaggccaa
      121 cgactgtcac tactgaatag cggcgcacct acacgacctc cgggatgagg tcgaagacga
      181 agcggtggtc gaccggatcg cggagggttg gaagaccgcc gacctcgacc cggtcgacgt
      241 ggccctgtgc gagtacaccg agaaactcac ccgcgccccc gccgcggttg ggcccgagga
      301 catcgaggcc cttcgccggg ccgggctcga tgaccagggc atctcgagcg ccacacaggt
      361 catcgcctac ttcaattaca taaaccgcat tgcagagggt ctcggcgtcc ccatggagac
      421 gtggctgaac gacgacggga ccctcgagga tgcctagcct cctcgcccat ggccaaggac
      481 aagaagaaaa agaagaagcc caagcccaag cacccgcccg agttgaagga ccgcaaggta
      541 agcaagcagt tcgagggcac gttcgggggg aagaaggtct cgtacacgac cacggccgcc
      601 acccaggtgt tcgagtcgaa cgacgagaag gtgtccttct tctacgtctc ctacacccgg
      661 gacgaggccg acccggcgga gcgaccggtg ctgttctgct tcaacggcgg gcccggatcg
      721 tccaccgtgt ggctcaacct agggctctat ggccccaagc gcatggagtt cgacgacgag
      781 ggcttcaaga cggggatgca gggccgtctc gtcgacaacg agcactcgat cctcgacgtg
      841 accgacgtcg tctgcatcga cgccatgggg accggtttca gcaggacggc cgagaaggac
      901 agcgaaacca agttccacca cttcaagaag gacatcgagg ccttcagcga gttcatcgtc
      961 cactacctca accgcaacgg ccgctgggcc tcacccaagt atctctccgg cgagagctac
     1021 ggaacgttgc gcggcgcagg catcgcccgc gagctgttcg ccacccacaa cgtggagttc
     1081 aatgggatcg tcttcatctc gatgatcctc aactaccaga cctcgggcat cgacgcagac
     1141 aacatgctga tccatcccgg caacgatctt cccttcgccc tgtatctgcc gacgtacgcc
     1201 gccaccgccc ggtaccacgg ccggttgtcc gagaagtacc agaagatggc gctgcccgac
     1261 tttctcgacg aggtggagga gttcgccctc ggtgagtact ggtcggctct ggccctgggc
     1321 gaccggatcg acgacgggcg caagaaggag gtcgccgagc gtctcagtgc ctacaccggt
     1381 ctcgacgccg ggttcatcga tcactacgac cttcgaatcc agattcatcg agtctgcaag
     1441 gaactgctgc gcgaccagca tcggacggtg ggccgcatcg actccagata tgtcgggatc
     1501 gaccgggtca aggcggggaa cgtgatcgag acagacccca gcctcgatgc caccggcggg
     1561 gtggccgcct cggcgctcaa ccactacctc cgcgacgaga tcggcttcga tacccatgac
     1621 atctacagga tcatgggacc gaacgtctgg aagaactggg actacgagga cttcaagggg
     1681 cgctacgtcg acacctcgga gaacttccgt gagctgctct cccgctcccg ggggaccaag
     1741 gtgctggtgg ccaatggcta ctacgacctc gccacaccac acttcgccac cgagtacacc
     1801 ttctcgcaca tgggtctcga cccggaggtt cgcgagaacg tggcgctgac ctactacgag
     1861 gccggccaca tgatgtatgt gcacaagccg tcgctcgagc agctggccac cgatctcaga
     1921 gagttcgtca ccaccacttc ctaagcctgt ggagccggat atccggctct gacggataca
     1981 gaaagggact tgaccaagcc atcgcagcct cgcataattc aacaagcgtt gaattaggag
     2041 ggactgtgct ggcgatgatc ctcaaggagt tccggcaggt tggccgggac cggcggaccc
     2101 tggcgatgct catcgtcatg ccggtcgtcc tcctcctggt gttcggctac gcggcgagct
     2161 tcgacgtcga ggagatcccc accatcgtcg tcggcgagaa cgccgagttg gccgccgatg
     2221 ccctccccga gatcctggtg gtcgaggaat cggatgccgc agcgacgcgc gaagaggcga
     2281 ttgccggtct ggtcgccggc cgagcccatg tcgccgtcgt ggccgatggg tcagacccgg
     2341 tggtgctcat cgacgggtcg gagctcttca tggcgagggc ggcggtccag gtggccacat
     2401 ccatgtcatc gcgagtgccc ggcttgaccc atgaggtcct cttcaatccc gagctggaca
     2461 catcggccgc cctggtcccc ggtctcgccg ggctgatcat cgtcttcgtc ggcaccctgg
     2521 tgacgtcgat gtcggtggtg agggagcggg aggccggcac cctcgagcaa ctcgccgtga
     2581 tgcccttcaa gcccaaggac gtcttcgtcg gcaagatcgc cccctacttc ctgatcgcgg
     2641 ccttcgacat cgtgatggtc ctgatcatcg ggaccctgat attcgacgtc ccgttcaacg
     2701 ggtcggtctg gcccc
//
LOCUS       DASUTA010000358         3055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1270852, whole genome shotgun sequence.
ACCESSION   DASUTA010000358 DASUTA010000000
VERSION     DASUTA010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270852"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VFZ80_07165"
     CDS             <1..383
                     /locus_tag="VFZ80_07165"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX6287249.1"
                     /translation="IIFLGLVYLFVWQVARAIGSHLGISVKRKRKEGSRVLFVRSESQ
                     KGKEFEVKDVVVMGRSEETDVVLDDPYASEFHLRFVSHENGMMLHDLGSTNGTYVNGR
                     RISAPTQLRQGDTVQVGKTVMEIR"
     gene            380..883
                     /locus_tag="VFZ80_07170"
     CDS             380..883
                     /locus_tag="VFZ80_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6287250.1"
                     /translation="MTTEIPVDARLERAVALREQGRDAEARSLLLELHDEFPGDARVN
                     LQCAWIHDKLGLESEAVPYYERAIAAGLEPDDRNDALLGLGSTYRALGRYDDAVQTLS
                     RAAHEFPDDRGVQAFLALAEYNTGAAKQAVERLLSLLIETTTDPGISRYEAALTEYAA
                     DVDRTWS"
     gene            880..1587
                     /locus_tag="VFZ80_07175"
     CDS             880..1587
                     /locus_tag="VFZ80_07175"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEX6287251.1"
                     /translation="MRYLFSTATHKGMVRDNNEDAIFPSSSGESTDHLLVIVADGMGG
                     HVAGEVASRLAINAAASSELDPADRVAAGNRAIREEVAREPELEGMGTTMTLLAIEGT
                     TATIGHIGDSRAYLLREGDFSQLTEDHTVAAEYVALGQLDPEDAATHPQRHMLTRTLG
                     LTRFVNVDEFKFDVLAGDRILLCSDGLTEMVSDEAIGSTLGNGTLEEIVWKLVEMAND
                     AGGVDNISVVAVEAVSD"
     gene            1592..2887
                     /locus_tag="VFZ80_07180"
     CDS             1592..2887
                     /locus_tag="VFZ80_07180"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX6287252.1"
                     /translation="MERRAEAITTAAAALLAAFAVSLVNFANGGSVDAQTGLTFLVFL
                     LAFGTIFVAIRAFAPRATPLLIPPVVALIAIGFLEIYRLAPERAGLQRWWLLIASAVA
                     AVTLMLLARGTTSILRRYRNLILLLSIGLLLLPNLPSDWGLPLRGLEVNGSRLWIVLD
                     LSFTQMQFQPAELAKLGMVIFFAGYLADHQRALREAHRRIGPIRLPEPRQLIPLLLAW
                     GGSIFILIWQRDLGASILLFAGFVLLLYAATGATGYLVAGGLMTLTGAAAAAYLFDHV
                     QRRIVAWLAPFEHYQDEGFQIAQGLFALGTGSLTGAGPGLGRPDLIPNASTDFIFAAV
                     GEELGLAGTVAVLALFALVIAVGFGISFRSRDTFRKLLAAGLTFVMAVQVFLIVGGVL
                     RLVPLTGITLPFMSYGGSALVGNMVLLVLLLRISHEEAT"
BASE COUNT          542 a          994 c          938 g          581 t
ORIGIN      
        1 ggatcatctt cctgggcttg gtctacctct tcgtctggca ggtggcccgt gccataggct
       61 cacatctggg gatctcggtc aagaggaaac gaaaggaagg gagcagagtt ctcttcgtgc
      121 gatccgaaag ccagaagggc aaggaattcg aggtcaagga cgtggtggtg atgggtagat
      181 ctgaggagac cgacgtggtc ctcgacgatc cctacgcttc tgagttccac ctgcgattcg
      241 tttcccacga gaatggaatg atgctgcacg atctgggcag caccaatggc acgtatgtca
      301 acggcaggcg gatatccgca cccacacaac tgcgacaggg cgacacagtc caagttggaa
      361 agaccgtcat ggagatcaga tgacgaccga gatcccggtt gacgcccgtc tggagcgggc
      421 tgtcgcgctg cgcgagcagg gccgcgatgc cgaagcccgc tctcttctcc tcgaactgca
      481 cgacgagttt cccggcgatg cccgggtcaa cctgcagtgt gcctggatcc acgacaagct
      541 cggtttggag agcgaagccg tcccctatta cgagcgcgcg atcgccgcag gtctcgaacc
      601 cgacgacagg aacgacgccc ttctcggcct gggcagcacc taccgggccc tgggacgcta
      661 cgacgacgcc gtccagaccc tgagccgggc tgcgcacgag tttcccgacg acaggggagt
      721 ccaggcattc ctcgccctgg ccgagtacaa cacgggagcg gccaaacagg cggtcgagcg
      781 gctcctctca ctcctgatcg agaccaccac ggatcccggt atctcgagat acgaagccgc
      841 tttgaccgag tacgccgcgg acgtggaccg aacctggtca tgagatatct cttctcgacc
      901 gcaacgcaca aggggatggt gcgtgacaac aacgaagacg ccatcttccc gtcttcctcg
      961 ggcgagtcca ccgaccatct cctggtcata gtcgccgacg gaatgggcgg acatgtcgcc
     1021 ggcgaagtcg cctccaggtt ggcgatcaac gcagccgcgt ccagtgagtt agacccggcg
     1081 gaccgggtcg cggcaggaaa ccgagcgata agagaagagg tcgcacgaga acctgagctg
     1141 gagggaatgg gaaccacgat gactcttctc gccatcgagg gaactaccgc cactatcggt
     1201 cacattggcg acagccgggc ctacctgctg cgtgagggag acttcagcca actcaccgaa
     1261 gaccacacgg tggctgcgga gtatgtcgct ctcgggcagc tcgaccccga agatgcagcc
     1321 acccacccgc agagacacat gctcacgagg acacttggcc tgacccggtt cgtcaacgtc
     1381 gacgagttca agttcgacgt tctcgcggga gaccgcatcc tcctctgctc cgacggcttg
     1441 accgaaatgg tctccgacga ggccatcggc tccacactgg gcaatggcac cttggaggag
     1501 atcgtctgga agctcgtcga gatggccaac gacgcaggcg gggtcgacaa catcagcgtc
     1561 gtcgcggtcg aagccgtctc agactgagcg catggagcgt cgagccgaag ccataaccac
     1621 cgcagcagcg gctttgctcg cagcctttgc ggtgtccctg gtgaacttcg ccaacggtgg
     1681 cagtgtcgat gcgcagaccg ggctcacgtt cctcgtcttc cttcttgcgt tcggcaccat
     1741 cttcgtggcg atcagggctt tcgctccccg ggcaactcca ctgctcatcc ccccggtggt
     1801 tgccctgatc gccatcggtt tcctggagat ctacaggctg gcccccgaga gagcaggatt
     1861 gcagcgctgg tggctgctga tcgcgtcggc cgtggccgca gtgacgctca tgctcctcgc
     1921 tcgggggacc acctcgatcc ttcgccgcta ccgaaacctg atcctgctgc tgtccattgg
     1981 cttgctactg ttgccaaatc tcccatccga ttggggtctg cccctgcgcg ggctcgaggt
     2041 caacggctcc cggctctgga tcgtgctcga cctttccttc acccagatgc agttccaacc
     2101 cgccgagctg gcaaagctgg ggatggtcat cttcttcgcc ggctacctgg cggaccacca
     2161 aagagcgctg cgcgaggcac accggcgcat cggcccgatt cgcctgccgg agccacggca
     2221 actgattccc ctgttgctgg cctggggtgg gagcatcttc atcctcatct ggcagcgcga
     2281 tctcggagcc tcgattctcc tgttcgccgg gttcgtcttg ctcctgtacg cggcgaccgg
     2341 agccaccggc tacctggtgg cgggcggtct gatgaccctc accggtgccg cggcagcggc
     2401 ctacctcttc gatcacgtgc aacgacgcat cgtggcctgg ttggccccat tcgagcacta
     2461 ccaggacgag ggctttcaga tcgcccaagg cctgttcgca ctgggcaccg ggtctctcac
     2521 cggggccggg cccgggctgg gacgacccga tctcatcccc aacgcctcca cggacttcat
     2581 cttcgccgca gtcggcgagg agctcggcct cgccgggact gtcgcggtgc tcgccctctt
     2641 cgccttggtg attgcagtgg ggttcggcat ctctttccgg agccgggaca ccttccgcaa
     2701 actgctcgcc gccgggctca ccttcgtcat ggcggttcag gtctttctga tcgtgggtgg
     2761 agttctcaga ctcgtcccac tgaccggcat caccctcccc ttcatgtctt acggcggctc
     2821 ggcgctggtg ggcaacatgg tgcttctcgt cttgcttctc aggatcagcc atgaggaggc
     2881 gacgtgaacg ggccgatccg gcgggtcgcc atcggagtct tcgtgtgcat gtcgctcttg
     2941 ctcgccgggg tgacctggta tcaggtcgtg agggcggatg agcttcgcga cgaccagcgc
     3001 aacccccggc ccctgttgtt ggagcgcgga aaagagcgcg gtctgatcgt cacag
//
LOCUS       DASUTA010000359         3152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_752523, whole genome shotgun sequence.
ACCESSION   DASUTA010000359 DASUTA010000000
VERSION     DASUTA010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752523"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VFZ80_07185"
     CDS             complement(<1..419)
                     /locus_tag="VFZ80_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEX6287253.1"
                     /translation="MADVTLFGRVDTTRYPISGGLLMGEIFNVLVDALGSVLAFFFDF
                     LHSFMDDGVAYGISIILLTIVINIVVFPLTLKQTRATRAFQALQPEIKRIQAEYKDDP
                     QEMQKRLMAAQKEAGATPGGCLLPLLVQMPIWFALFRL"
     gene            complement(346..597)
                     /gene="yidD"
                     /locus_tag="VFZ80_07190"
     CDS             complement(346..597)
                     /gene="yidD"
                     /locus_tag="VFZ80_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003023846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX6287254.1"
                     /translation="MPEALRPRSWVVAAIYVYQKLVSPNLGANCRYQPTCSSYAAEAI
                     ARFGVLKGGWLGMKRIGRCHPLRPGGHDPVPDQWRASNG"
     gene            complement(590..958)
                     /gene="rnpA"
                     /locus_tag="VFZ80_07195"
     CDS             complement(590..958)
                     /gene="rnpA"
                     /locus_tag="VFZ80_07195"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX6287255.1"
                     /translation="MVAKRAESGSLPSSSTGYESLRNSRDFRRVLNDGTRRRIGGIVV
                     VDSPGKTGPPRVGLVVSRSCGSAVARNRIKRRLRSAASAIELEPGIDYVIIANRQVAE
                     APYEQLVGWLRRATSEDGHA"
     gene            complement(921..1055)
                     /gene="rpmH"
                     /locus_tag="VFZ80_07200"
     CDS             complement(921..1055)
                     /gene="rpmH"
                     /locus_tag="VFZ80_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004823743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX6287256.1"
                     /translation="MKRTYQPKVRRRKRRHGFRHRMRTRAGRATIKNRRQKGRKRLAA
                     "
     gene            1548..2924
                     /gene="dnaA"
                     /locus_tag="VFZ80_07205"
     CDS             1548..2924
                     /gene="dnaA"
                     /locus_tag="VFZ80_07205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX6287257.1"
                     /translation="MHPERADDRWADFRGALKAGVSESNWETWLSRLEPESEGERLTL
                     VAPSEFHLQWVRDKHGDLIASTYRQYFGGEPAYRVNDAAPSEQPLPLPTEEPPPSAEV
                     RDDAASPSRATNLMGRYRFESFVVGQSNRFAWAAAMAVTEQPGTHYNPLFIYGSAGLG
                     KTHLLNAVGHQALEMYPGLVVRYVSSENFLNDFIESIRRKRPDDFKSRYRGVDVLLLD
                     DVQFFEGKEQILEEFFHTFNSLYESGKQMVISSDRHPRNLSSLEDRLRSRFEWGLLTD
                     IQPPDMETRIAILRRNAEFAPRPVPVDVLEYIAGLVSDNIRELEGALTRVTAWSALNR
                     QDIDLETAERVLKDMVTTDEPVPLGPETIIRTTATAYSFSVEDVLSSSRKQPLVLCRQ
                     ISMYLCRELTDLSLPKIGEHFNRDHTTVLHSVEKVKRILRSDRAVFDRVNQLTQDLRK
                     GGGVSTGS"
BASE COUNT          685 a          969 c          907 g          591 t
ORIGIN      
        1 agtcgaaaca gcgcaaacca gatcggcatc tgcaccaaga gggggagcag acagccaccg
       61 ggggtggccc cggcctcctt ctgcgccgcc atgagacgct tctgcatctc ttgtgggtcg
      121 tccttgtact cggcctggat ccgcttgatc tctggctgca aggcctggaa ggcgcgagtg
      181 gcacgggtct gcttgagcgt gagcgggaac accacgatgt tgatgacaat cgtgagcagg
      241 atgatggata tgccgtaggc gacaccatcg tccatgaagc tgtggagaaa gtcgaagaaa
      301 aaggccagga ccgacccgag cgcatcaacg agaacattga agatctcacc cattagaagc
      361 cctccactga tcgggtaccg ggtcgtgtcc acccggccga agagggtgac atctgccaat
      421 gcgcttcatc cccaaccatc cacctttgag aactccaaac cgagctattg cctcagccgc
      481 gtaggaggag caggtcggct gataacgaca gttggccccg aggttgggac ttaccaattt
      541 ctggtaaacg tagatggcag cgaccaccca ggaccgtggg cggagagcct caggcatggc
      601 cgtcttcgga cgtggcacgg cgcagccagc cgaccagctg ttcgtagggt gcttctgcaa
      661 cctgtctgtt ggcgatgatc acgtaatcaa ttcctggttc gagctcgatg gcactcgccg
      721 ccgagcgaag tcgtctcttg atgcgattcc gggcgacggc tgagccgcaa ctcctggaga
      781 ccaccagccc gacacgcggg ggccctgtct ttcctggcga gtcgactacg actatgccac
      841 caattcggcg ccgggtccca tcgttgagaa ctctacgaaa gtcgcgagag ttgcggaggg
      901 attcgtagcc ggtcgatgag ctaggcagcg agccgctttc ggcccttttg gcgacgattc
      961 ttgatcgtcg cccgcccggc gcgcgttcgc atccggtgac ggaaaccatg acgtcgcttt
     1021 ctgcgacgga ctttgggttg ataggtcctc ttcatgacaa atcaaggggg ttggggaggg
     1081 gaactcgcac agactacgga aacaccggta gttgcgcaat gtgagctgag cccacctgac
     1141 gtcccaggca tggccgaaat gagacggacg tatcaattag cgcccctcgt cgcgaagaaa
     1201 cgacataagg cgcgaaaaat cgattaggcg gaattggtcg ggtcttgccc ggggggaagc
     1261 caaccatcat ctggaaaatg accgggctag cagggaaatc gttgtggata cctcggcaag
     1321 ggttcatcca caacggcgat tctcgcgttc ttgactcgtc tcatacggca tctaatactc
     1381 gctcgactca cccgggagtg ccccacttcg gtgtcgttgc cggatgacac ggcgaccaac
     1441 ccgggccgac aagtaagcga atcgctggtg gatgtgcaca gagatttcca catgtgtgga
     1501 ctgttctgtg gacaaaccgg cccgtgggag gagtggcctt caaattgatg catcccgaaa
     1561 gagctgacga cagatgggct gatttccgtg gtgccttgaa ggccggtgta tccgagagca
     1621 actgggagac ctggctttcc cggctcgagc ccgaatccga aggtgagcgg ctgactctcg
     1681 tcgcccccag cgagttccac ctgcaatggg tgagagacaa acacggcgac ctgatcgcct
     1741 ccacataccg tcagtatttc ggaggcgaac cggcatacag ggtcaacgat gctgctccct
     1801 ccgagcagcc gttgccgctc cccactgagg aaccgccacc ttcggcagaa gtcagagacg
     1861 atgcggcttc gccgtctcgc gccaccaacc tcatgggccg ctaccgattc gagagtttcg
     1921 tggtcgggca gtcgaatcga ttcgcctggg ccgcggccat ggcggtcacc gaacagccgg
     1981 ggacccacta caacccgctg ttcatctatg gaagtgccgg gctcggtaag acccatctcc
     2041 tcaacgctgt cgggcatcag gcacttgaga tgtatccggg gctcgtcgtt cgctacgtct
     2101 cctccgagaa cttcttgaac gacttcatcg aatcgatccg tcgaaagcga ccggatgatt
     2161 tcaagtcgcg gtatcggggg gtcgacgtcc tcctcctcga cgatgtccag ttcttcgaag
     2221 gcaaggagca gatcctcgag gagttcttcc acaccttcaa ctccctctac gagtcgggca
     2281 agcagatggt catcagctcg gaccgccacc cccgcaatct ctcatccctc gaggacaggc
     2341 tgcgcagcag gttcgagtgg gggctcctga ccgacatcca gccccccgac atggagaccc
     2401 ggatcgccat tctccgccga aacgcggagt tcgccccacg gccggtccca gtcgatgtgc
     2461 tcgagtacat cgcgggcctg gtctccgaca acattcgcga gctcgaaggc gctctcaccc
     2521 gggtcaccgc ctggtcggcc ctgaaccgtc aggacatcga tctcgagacc gccgagcgtg
     2581 tgctcaagga catggtcacc accgacgagc ccgtaccgct cgggcccgag accatcatcc
     2641 gaaccaccgc caccgcctac agcttcagtg tcgaagacgt gctcagctcc agtcgcaaac
     2701 agcccctggt gttgtgcagg cagatctcga tgtatctctg ccgagagctg accgacctct
     2761 cgcttcccaa gatcggcgag cacttcaacc gcgatcacac cacagtcctg cactcggtgg
     2821 agaaggtgaa acggatcctg aggtccgatc gcgccgtgtt cgaccgtgtg aatcaactca
     2881 cacaggacct gaggaaaggt ggtggtgttt ccacaggctc ctgagttata caccggctct
     2941 ccccagatcg cgaagaatcg tctacaggga agtccacaag agaaccgtca agcgtcaact
     3001 gcccaaaacc gacctatcca cacttccaca accccctact acaactaata acgtttcaat
     3061 gaagatgagc agcaacagaa gaagggacaa ctgcctgtga gaatccgagc tcagcgagac
     3121 gatctcgccg acttactcgc acgagcgaac cg
//
LOCUS       DASUTA010000360         3026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_106138, whole genome shotgun sequence.
ACCESSION   DASUTA010000360 DASUTA010000000
VERSION     DASUTA010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106138"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /gene="engA"
                     /locus_tag="VFZ80_07210"
     CDS             <1..157
                     /gene="engA"
                     /locus_tag="VFZ80_07210"
                     /inference="COORDINATES: protein motif:HMM:NF026064.4"
                     /note="synchronizes cellular events by interacting with
                     multiple targets with tandem G-domains; overexpression in
                     Escherichia coli suppresses rrmJ mutation; structural
                     analysis of the Thermotoga maritima ortholog shows
                     different nucleotide binding affinities in the two binding
                     domains; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome-associated GTPase EngA"
                     /protein_id="HEX6287258.1"
                     /translation="QAGTAPPTFVLFVSGGELGDDYLRFLENRLRAEYDFTGNPIHFV
                     TRNRAER"
     gene            154..285
                     /locus_tag="VFZ80_07215"
     CDS             154..285
                     /locus_tag="VFZ80_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287259.1"
                     /translation="MTDQHDQEDVKTPLSFRIMIVLVVLYLGWRLVEVIVWLWQRLF"
     gene            463..765
                     /locus_tag="VFZ80_07220"
     CDS             463..765
                     /locus_tag="VFZ80_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287260.1"
                     /translation="MGAVIIAVLFVAGGIFFAAFTGGLASTIIGGGREDNSLLTNAFI
                     GFIGWLIAGFIWAQTYGGWPEELSIGFLFLTLACSVGVAWFRGRRIRRAEKQDISS"
     gene            complement(755..1261)
                     /locus_tag="VFZ80_07225"
     CDS             complement(755..1261)
                     /locus_tag="VFZ80_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287261.1"
                     /translation="MIDDRRSEVRIKGNAVRLGLDIDHLEPAEVAPVDLAESDAREES
                     LRTAAPALPSAWLSMRGIAVALATDSQPYDLVVTLPDGIKRVQVKTTTSRRDSTWLAG
                     VGHRPYSGDAREGKLPYDPASLDPFAIINGDGEIDLVPVGAIAGRTGIYLSAYERYRV
                     GDVSSLMS"
     gene            complement(1537..1614)
                     /locus_tag="VFZ80_07230"
     tRNA            complement(1537..1614)
                     /locus_tag="VFZ80_07230"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1577..1579),aa:Pro,seq:ggg)
     gene            1727..2878
                     /locus_tag="VFZ80_07235"
     CDS             1727..2878
                     /locus_tag="VFZ80_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010278847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6287262.1"
                     /translation="MSTSDRLEPFGVTIFTEMSALALEHDAINLGQGFPNWDGADFVK
                     EAARKSMADGGHDQYPPMPGIPELRGALAERYSGLLGREIDMGTEVTVTSGCTEALAA
                     ALLGLVNSGDEVILIEPYYDAYPADASMAGAVPRYVSLRPPGFELDLDELASLFSDKT
                     KAIVVNNPHNPTGRVFTDVELAAIARLCVEHDVIAICDEVYEEMTYGVTHLRLATYPG
                     MWERTLTLSSLGKTFSLTGWKVGWAIGPPHLSAGLRSAHQFLTFTTPTPVQHGAAAAL
                     GAEPRFYEEMRSSYRAKRDLLAAGLSDTGFEVHLPMGTYFLMAGHAEAADDRAFCRRL
                     VVEFGVAAIPPSVFYSDPGRGSGLVRFAFCKDEATLEEAIERISGIAQP"
BASE COUNT          545 a          958 c          923 g          600 t
ORIGIN      
        1 gcaggcgggc acggcgcccc cgaccttcgt cctgttcgtt tcgggcggag agttggggga
       61 cgactacctc cgcttcctcg agaaccggct gagggcggag tacgacttca cgggcaatcc
      121 catccacttc gtgacacgca accgggccga gcggtgaccg atcagcacga ccaggaggat
      181 gtcaagactc cgctgagctt caggatcatg atcgtactcg tcgtgctcta tctgggttgg
      241 cgtctcgtcg aggtcatcgt ctggctctgg caacggctgt tctgaaacag cgcaggtcca
      301 tggcgaatcg acttcgccac ctttctgtga gggagtcgtc gccgcgcctg gctccgccag
      361 ggtctatacc tggaattggg actcgaacag gtctatccag tcggatgccg acttcgtaga
      421 atcaaagggc cgcacctaga acggcagaaa gacagctcgg aattgggtgc cgtgatcatc
      481 gcggtgttgt tcgtggcggg tgggatcttc ttcgcggcct tcaccggggg actggcaagc
      541 accatcatcg ggggcggtag agaggacaac tccctcctca ccaacgcctt catcggtttc
      601 atcggctggc tgattgccgg gttcatctgg gcccagacgt atgggggctg gccggaagag
      661 ctatccatcg ggttcctgtt cctcacgctc gcctgctccg tcggcgtcgc ctggtttcgg
      721 ggcagacgga ttcgccgggc tgagaagcag gacatcagct catgaggctc gagacatctc
      781 ccactcggta gcgctcgtat gcgctcaagt agatcccggt ccttcccgca attgctccta
      841 ccggtaccaa gtcgatctct ccatcgccat tgatgatggc aaaggggtcg aggctcgcgg
      901 ggtcgtacgg cagcttgccc tcgcgtgcat cccccgagta gggccgatga cccacgcctg
      961 cgagccaggt gctgtcacgc cgggaggttg tcgtcttgac ttggactctc ttgatgccgt
     1021 ccggaagcgt gacgacgagg tcgtatggct gagaatccgt ggccaaggcc acggctattc
     1081 ctcgcatcga caaccaggcc gatggcaatg caggcgcagc ggttctgagg ctctcttctc
     1141 tggcgtcgga ttcagctagg tcgaccggcg cgacctctgc cggttcgagg tggtcgatgt
     1201 ccaatccgag ccgaaccgca ttgcccttga ttctgacttc cgagcggcga tcgtcgatca
     1261 actcgatgcg ccgaaggact tcgccccacg atttgattcg gtgactgctc ggaccagttg
     1321 acggtcggac cagcgccgag gtccagcaaa atgtgacgtg tcgaggccaa ggcggctcga
     1381 gtgcctccgc acaacttgta tcgacccccc ggagtcactg tctaggccga gagctcgaag
     1441 aacaccgcgc cacgactcct caacggcacg tcgctactcc gtccatttga ctccactgat
     1501 accgccgaca gtagaacggc cttgtgacaa gaattctggt cgggccggcg ggatttgaac
     1561 ccgcgacctc gtgtccccca gacacgcgcg cttaccaagc tgcgccacgg cccgtgatta
     1621 catggccgct caaagtgcgc catgtcccgt gtttggctga gccgaggata ccccacgcac
     1681 gtgatcacgg ccagaaccgg gccgccggct ccctaatgtc tccgccgtgt ccacatcaga
     1741 ccggctcgag ccattcggcg tcaccatctt caccgagatg tctgctctgg ctctggaaca
     1801 cgatgcgatc aacctgggtc agggctttcc caactgggac ggtgccgact tcgtgaaaga
     1861 agctgcccgg aaatccatgg cagacggcgg ccacgatcag tacccgccga tgccagggat
     1921 ccccgagctc cgaggcgccc tcgccgagcg gtacagcggt ttgctcggtc gagagatcga
     1981 catgggcacc gaggtcacgg tcaccagtgg atgcaccgag gcactggctg ccgccctcct
     2041 cggcttggtc aactccggtg acgaggtgat cctgatcgag ccttactacg acgcataccc
     2101 cgctgacgca tcgatggcgg gtgcggttcc tcgttatgtg tcgctgcgcc cgccgggctt
     2161 cgagctcgat ctggacgagc tggcatctct attcagcgac aagaccaaag cgatcgttgt
     2221 caacaaccct cacaacccca cgggccgggt cttcaccgac gtcgagctgg ctgcgatagc
     2281 caggctctgt gtcgaacacg acgtgatcgc catctgtgat gaggtctacg aggagatgac
     2341 ctatggcgtt acccatcttc gactggccac ctatcccggt atgtgggaac gaacgctgac
     2401 cctctcgtcg ctgggaaaga ccttctcact gacaggctgg aaggtgggat gggccatcgg
     2461 tcctccacac ctgagcgccg gtctgcgctc ggctcatcag tttctcactt tcaccacgcc
     2521 cacaccggtg caacacggcg cggcggcggc cctcggcgca gagccacggt tctatgagga
     2581 gatgaggtcc tcctaccggg cgaagcgaga tcttctcgcc gccggtctct ccgacacggg
     2641 cttcgaggtg catctgccga tgggaaccta cttcctgatg gcgggtcacg ccgaggcggc
     2701 cgacgacagg gctttctgcc gccgcctcgt ggtcgagttc ggggtggcag cgatcccacc
     2761 cagcgtcttc tacagcgatc ctgggcgcgg atcggggctg gtcaggttcg ctttctgcaa
     2821 agacgaggcg actctcgagg aagcgatcga gcgcatctca ggtattgctc aaccgtaggg
     2881 ggtgcatgcc gatcttctga tgggaggttg gtttgcaaga caagaccctc agaacaattg
     2941 gagcttcggt agtcctcttc ggtatcgccc tggccggcat cgcccaggcc ggctccgccg
     3001 agaaccagac gccgactgag atcgtc
//
LOCUS       DASUTA010000361         2299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1401908, whole genome shotgun sequence.
ACCESSION   DASUTA010000361 DASUTA010000000
VERSION     DASUTA010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401908"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VFZ80_07240"
     CDS             <1..648
                     /locus_tag="VFZ80_07240"
                     /inference="COORDINATES: protein motif:HMM:NF016557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287263.1"
                     /translation="MVFDIIFLDGKDLTGRPLAARRLILEEAMVVSDRIQISPATEGS
                     GVALFEAAAAQGLEGIMAKRKSSTYRPGARSKDWLKAKVTFDADVVIVGWTEGEGGRA
                     GTLGSLVMAVYDGDELRYVGNVGTGFDKRSLSETKEQLEALQETEAPFPADVVRSRTE
                     LRRAHWVEPDLVARVEHRQLTEAGRLRAPSFQGFREDKAPRDCTWEQLASESSLS"
     gene            659..1138
                     /locus_tag="VFZ80_07245"
     CDS             659..1138
                     /locus_tag="VFZ80_07245"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX6287264.1"
                     /translation="MPDISGLVDAVRRHYDDGCFACGRENPIGLHLDDFSLNDGRVCA
                     RFTPRPEYRGAGKTLHGGIAATALDEILVWAGILTESVLTVTGTLEIRFRRPVRVDDP
                     IAVSAVVDGRSGRRLQISGSLAVGEETRVEAAGLYLVSMSLDEILAGHRVEGQGEPA"
     gene            1207..1602
                     /locus_tag="VFZ80_07250"
     CDS             1207..1602
                     /locus_tag="VFZ80_07250"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6287265.1"
                     /translation="MSLRETLSGARRADETMYQHEQALLDPDVRGSRMRLESLFHADF
                     SETGSSGEVYDRATMIEMMVGESPGNVIMRDFTTSYLSADVALVTYRSVGTGGQEARR
                     SSVWVFEDERWQLRHHQGTRVPDRWSRVH"
     gene            complement(1617..2165)
                     /locus_tag="VFZ80_07255"
     CDS             complement(1617..2165)
                     /locus_tag="VFZ80_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287266.1"
                     /translation="MPTPVSPSSMEGRRVIVEFRAATPAEVRAIDTTADALVLPAHLL
                     MTKAEWRRANSALPAHHPTLHSRSNRPPGSLIWAQLASRSGSMMVGGRDSVGMPSVPL
                     PAQSGDIVFVSTVPAEGARPIATARPLPTMAVEHVIVEPDACQPIIAADGEIICGKST
                     CASCLTVLTRNAWYSSIACLCE"
BASE COUNT          423 a          703 c          771 g          402 t
ORIGIN      
        1 atggtgttcg acatcatctt cctcgacggc aaggacctga ccggtcgccc attggcagcg
       61 agacgtctca tcctcgagga agccatggtg gtctcggacc gcatccagat ctcgccggcc
      121 accgagggaa gtggggtcgc actcttcgag gctgcagcag cacagggtct cgaagggata
      181 atggccaaga ggaagtcctc gacataccgg ccgggagcac gctccaagga ctggctcaag
      241 gcaaaggtga ctttcgatgc cgatgtcgtc atcgtcggct ggacagaggg cgagggtggg
      301 cgcgccggga cgctcggctc gttggtgatg gcggtatacg acggcgacga gctgcgttat
      361 gtgggaaacg tcgggaccgg tttcgacaag cgatccctct ctgagaccaa ggagcaactg
      421 gaagccctgc aggagacaga ggcgcctttc cctgccgacg tcgtgcggtc acggacagag
      481 ctgcgccggg cgcactgggt cgagcccgat ctggttgctc gggtggaaca tcgtcaactc
      541 accgaggccg ggaggttgcg ggcgccctcc ttccaggggt tccgggagga caaggcaccg
      601 agagattgca catgggaaca gctcgcatcg gagtcgtcgc tctcgtagcg tggcgacgat
      661 gccagacatc tccgggctgg tcgacgcggt cagacgtcac tacgacgatg gatgctttgc
      721 ctgtggtcgg gagaacccga tcggtctcca tctcgacgac ttctccctga acgacgggag
      781 agtctgcgct cgcttcaccc ctcgccccga gtatcgcggc gcggggaaga cacttcacgg
      841 cggcatcgca gcaacggccc tcgacgagat cctggtgtgg gccggcatcc tgaccgagag
      901 cgtgctgact gtcaccggca cgttggagat ccgcttccgt cgacctgtcc gtgtcgatga
      961 cccaatagcc gtgtccgcgg tcgtggacgg tcgcagcggt cggcgtctcc agatctcggg
     1021 ttcactcgct gtcggcgaag agacccgggt ggaggccgcc gggctctatc tggtctccat
     1081 gtcgttggat gagatcctcg ccgggcatcg ggtggagggg cagggcgagc cggcctgact
     1141 gggccgttcg accgatatgg cgccacgctg tgacagtcga tactgatcct gtgaccgaag
     1201 gcaagcatga gcctgcgtga gacgctgtcc ggcgcccgac gcgccgacga aaccatgtac
     1261 cagcatgaac aagcgctgct cgacccggat gtgcgcggct cccggatgcg tctcgagagc
     1321 ttgttccacg ccgacttctc cgagaccgga agctcgggag aggtgtacga ccgagcgacg
     1381 atgatcgaga tgatggtcgg tgaatcgccc ggcaatgtga tcatgagaga cttcaccacc
     1441 agttacctgt cggcggatgt ggcactggtc acctaccgct cggtaggcac gggtgggcag
     1501 gaggccaggc ggagctctgt ctgggtcttt gaggacgagc gatggcagct gcgccatcac
     1561 caaggcactc gggtcccgga ccgttggagc cgggtgcact gacacccggg actcgcctac
     1621 tcgcagagac aggcgatcga cgagtaccag gcgttccggg tgagcacggt cagacagctt
     1681 gcgcaggtcg acttcccgca gatgatctcc ccgtcggccg cgatgatcgg ctgacaggca
     1741 tcgggctcga cgatcacgtg ctcgactgcc atggtgggga gcggtctcgc cgtcgcaatc
     1801 ggtcttgcac cttcagcggg caccgtcgag acgaacacga tgtccccact ctgcgcgggc
     1861 agaggcaccg atggcatccc cacactgtcc ctccccccga ccatcatcga gcctgagcgg
     1921 gaggcgagtt gtgcccaaat caacgaaccg ggcggccggt tggaccggct gtgaagagtc
     1981 ggatggtgtg ccggaagcgc tgagtttgcc cttcgccact ccgccttggt catcaacaga
     2041 tgtgcgggca ggacgagcgc gtcggcggtg gtgtcgatgg cacggacttc ggccggcgtc
     2101 gccgcccgga actctacgat cacccgcctg ccctccatgg aggaaggtga gacgggtgtc
     2161 ggcatcaccg atgtggtaac aggcataact gatagagccg ccagactcac ccctgataca
     2221 cgcgagggcc gtttcgaggc tttgctatgg catcaggact cggaaaccac gtcatcatgc
     2281 catagcaacc ccagtcccg
//
LOCUS       DASUTA010000362         2629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_493196, whole genome shotgun sequence.
ACCESSION   DASUTA010000362 DASUTA010000000
VERSION     DASUTA010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493196"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VFZ80_07260"
     CDS             complement(<1..598)
                     /locus_tag="VFZ80_07260"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287267.1"
                     /translation="MKVRTLCVWFPMWSLAVARKTRQDAPSGEPVLVADDRVTGATED
                     VLEAGVSLGMPRREAEALAPFATVLNRDIGEETRHFEPVVAVVEELVPRVEVVSPGLL
                     FVPVAGAVKFYGGEKALAVKVEEALREHLEDEDTSEASAGAVSIEGGRRLPIWQAGVS
                     LETPYRSSEAREAPSARYARYPPPELRSGNSSGWDHHLG"
     gene            complement(513..1241)
                     /locus_tag="VFZ80_07265"
     CDS             complement(513..1241)
                     /locus_tag="VFZ80_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287268.1"
                     /translation="MCSIGWIALRLESVLRIEGVTTAGVASSELGLTRAVLAGSRGLL
                     DLQPGRITELIGDPGTGLTRLGLELIAPYSSHAPVVALDTRGWISPEAAWETGVGSDR
                     FVVVRCDDPVLWVKVMASLLEGVRAVYAEVPARIRDHDLRRLAALARARRVAVAMRAM
                     GNGLPAGIAFLRLRALGVTWEGADRGHGRLTRRRLVLEASGKGVAGTLRRFEVEDEGT
                     DVMRVVSDVVVGSRPENAARRAVG"
     gene            1305..1658
                     /locus_tag="VFZ80_07270"
     CDS             1305..1658
                     /locus_tag="VFZ80_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6287269.1"
                     /translation="MPKILVADDSETIQTLLRTALGRAGYEVETAGDGPTAHSMAREG
                     GFDLIILDQLMPGLLGLDVISQLREDGVEAPVLMLTGVDDGTTAAESLDRGAVDYIRK
                     PFDMNELLARIKSRL"
     gene            1729..>2629
                     /locus_tag="VFZ80_07275"
     CDS             1729..>2629
                     /locus_tag="VFZ80_07275"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX6287270.1"
                     /translation="MLDIRIAEIGTLVIYLIDVVLLLLLVGLKTIHRRRVLHHEQRRQ
                     EYLRLLSRHVSYDDCTDPITPRVAEDPAFLDALIDVREVLSGHELNNLEAIVAEHGVG
                     ARLVKHLSSPFPLGRRLRAAVALAEIGDISVAPAMIAHLSDREPDIRIECARGLARLR
                     WTPAIDDIVARFGIEETAVRARFADSLVGFGTTATWPLLAYVRVNHRSEDAGPALALR
                     TVGRIRELGAARSILEVMGEKPSLEVMIAAIDALGDIASPDSTGRLQSLLADDRWQVR
                     AKAASALGRISESSSIPLLQTALQ"
BASE COUNT          495 a          926 c          761 g          447 t
ORIGIN      
        1 tgccgagatg gtgatcccaa cctgaagagt tcccagagcg aagctccggc ggggggtagc
       61 gagcgtagcg agcggagggt gcctctcgag cttcgctcga gcggtaggga gtttctaacg
      121 aaactccagc ctgccaaatg ggcaggcgtc gtcccccctc gatcgatact gcccccgcac
      181 tcgcttcgct cgtgtcttca tcctcaaggt gttctctcag cgcctcctcc accttcacgg
      241 ccagagcctt ctctcccccg tagaacttca cggctccggc cactgggacg aacaacagcc
      301 cgggcgacac cacctctacc cgtggcacca gttcctccac cacggccacc acgggctcga
      361 aatgtcgggt ctcctccccg atgtcacggt tgagcacggt ggcgaaagga gccagggcct
      421 cggcctcccg tcgtggcatg cccagggaga ctccggcttc gaggacatct tccgtcgccc
      481 ccgtcacacg atcgtcagcg accagtaccg gctcacccga cggcgcgtct tgccgcgttt
      541 tccgggcgac tgccaacgac cacatcggaa accacacgca taacgtccgt accttcatct
      601 tccacctcga aacgccggag agtcccggct acccccttac cgctcgcctc caggacgaga
      661 cgacgacggg tcaaccgccc gtgcccacgg tcggcaccct cccaggtgac acccaaagcc
      721 ctcaacctga ggaaggcgat acccgcgggc aatccgttgc ccatcgcccg cattgccacc
      781 gcaaccctcc tggcccgggc cagggcagcg agacgacgca agtcgtgatc acggatccga
      841 gcgggcactt cggcgtacac ggcgcgaacc ccctcgagaa gcgatgccat gaccttgacc
      901 caaagcaccg ggtcgtcaca cctgacgacc acgaaccgat cactgccgac accggtctcc
      961 cacgccgcct ccggtgagat ccatccccgg gtgtcgagtg cgaccacagg agcatgtgag
     1021 gaataggggg cgatcaactc gaggccgagc cgggtcagac cggtcccggg atcaccgatc
     1081 agctctgtga tccgaccggg ttgcaggtcg agaagacccc tcgagcccgc caggactgct
     1141 cgggtcagac ccaattcgga tgaagcgaca ccagccgtcg tcacaccctc gattcgcaag
     1201 acagactcca atctgagggc gatccatcct atcgaacaca tgttcgagtg gcaacaggca
     1261 accgaccctt cgccatccgg agtagcctct ctccattcag ggatatgccc aagatcctcg
     1321 ttgccgacga ctcagagacc atccagacct tgcttcgcac tgcattgggc cgcgccggtt
     1381 acgaggtcga gaccgcaggt gacggcccca ccgcccattc catggccagg gaaggcgggt
     1441 tcgatctcat catcctcgac cagctcatgc ccgggcttct cggcctcgac gtgatcagcc
     1501 agcttcgcga ggacggggtg gaggcgcccg tgctgatgct caccggagtc gacgacggga
     1561 ccacggccgc agagagcctc gatcgggggg cagtcgacta catccgcaaa cccttcgaca
     1621 tgaacgagtt gctggcgcga atcaaaagcc ggctctagac agacggtccc cctcctcccg
     1681 cggcgtttca cttcacgccg agccctgcgc agccgatatc agcatggaat gctggacatc
     1741 cgaattgccg agatcggcac tctggtcatc tacctgatcg acgtcgtcct tctcctgctt
     1801 ctggttggcc tgaagaccat ccaccgccga cgtgtcctcc atcacgagca gcgacgacag
     1861 gagtatctcc gcctgctcag tcgtcatgtc tcgtatgacg attgcaccga cccgatcacg
     1921 ccacgagtgg cggaggatcc ggcattcctc gatgccctca tagacgtacg ggaagtcctc
     1981 tccggtcacg agctgaacaa tctcgaggcc atcgtcgccg aacatggagt cggcgcccga
     2041 ctggtgaagc acctgagctc acccttcccg ctgggtcggc gactgcgagc cgcggtggct
     2101 cttgccgaga tcggggacat ctcggtcgca ccagcgatga tcgcgcactt gtccgaccgt
     2161 gagccggaca tacgaatcga gtgtgcccgg gggctggctc gtctcaggtg gactccggct
     2221 atcgatgaca tcgtcgcccg atttggcatc gaggaaacgg ccgtgcgagc ccgcttcgcc
     2281 gattctctcg tcggtttcgg caccacggcg acatggccct tgctcgccta cgtccgggtg
     2341 aaccatcgat ccgaagacgc cggtccggct ctcgccctca gaaccgtcgg gcgcattcgc
     2401 gagctcggcg cggctcgctc gatcctcgag gtgatgggag agaaaccgag tctcgaggtg
     2461 atgatcgcgg ccatcgacgc cctgggagac atcgccagcc cggatagcac gggaaggctc
     2521 cagtccctgc tggcggacga tcgctggcag gtgcgtgcca aggcggcgtc cgcgctcggg
     2581 aggatatccg agtcgtcctc gatccccctt ctccagacgg cgctgcaag
//
LOCUS       DASUTA010000363         2387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1012555, whole genome shotgun sequence.
ACCESSION   DASUTA010000363 DASUTA010000000
VERSION     DASUTA010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012555"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFZ80_07280"
     CDS             complement(<1..435)
                     /locus_tag="VFZ80_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX6287271.1"
                     /translation="MPPQNITPKKMPFEKYRPFVPLVLEDRTWPNVTIEEAPRWCSVD
                     LRDGNQALIDPMDPARKLRMFQELVAMGFKEIEVGFPAASQPDYDFVRQLVEDDLIPE
                     DVTIQVLVQCRDELIERTYDCISGAPRAIVHFYNSTNPLQRDV"
     gene            complement(641..796)
                     /locus_tag="VFZ80_07285"
     CDS             complement(641..796)
                     /locus_tag="VFZ80_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287272.1"
                     /translation="MRSSRRTTSPARRRLFGIRRQKPDTGQYEISIDEVMADLMGKGD
                     VRNWHLL"
     gene            complement(1067..1543)
                     /locus_tag="VFZ80_07290"
     CDS             complement(1067..1543)
                     /locus_tag="VFZ80_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017200634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6287273.1"
                     /translation="METWTVAIDDPRREDIVALLQRHLEFARGVTPPEDVHALDLDGL
                     LDPKVTFFSLRDDGRLLGIGAIKELGEGHMELKSIHTAEEARGNGVGRTVVNHLLSVA
                     AERGARRVSLETGSMEAFAPSRALYARVGFVECDPFGDYNPSRSSTFMTLALSPDG"
     gene            1619..>2387
                     /locus_tag="VFZ80_07295"
     CDS             1619..>2387
                     /locus_tag="VFZ80_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6287274.1"
                     /translation="MKFGLFLQPLHHVSEDPTKALERDLDLIVHLDQLGYSEAWIGEH
                     HSTGWENIAAPEVVIAAAAERTEQIRFGTGVVQAGLHHPLVALDRMILLDHLTRGRVS
                     FGIGVGGGIPSDLAVFGLSPESASRRMRESLDVMLRLLEGTEAVTEKTDWFELRDARL
                     QLRPYSTPHMPFAVASGDPENVAVMGRIGGKVLLGGTPDRVPQVLESLEKGAADAGIE
                     AGRDQIMLSYVMHLDESHRQALEDFESGAIREFYEFQV"
BASE COUNT          422 a          686 c          816 g          463 t
ORIGIN      
        1 gacgtcgcgc tgcagggggt tggtcgagtt gtagaagtgg acgatcgctc ggggggcgcc
       61 cgagatgcag tcgtatgttc tctcgatcaa ctcgtcccgg cattggacca ggacctggat
      121 ggtcacgtcc tcgggaatga gatcgtcttc gaccaactgg cggacgaagt cgtagtcggg
      181 ttggctcgcg gccggaaacc cgacctcgat ctccttgaat cccattgcca cgagctcttg
      241 gaacatgcgg agctttcgtg ccgggtccat ggggtcgatc agggcctggt tgccgtcacg
      301 gaggtccacc gagcaccacc gtggtgcttc ctcgatcgtg acgttgggcc aggtgcggtc
      361 ttcgagcacc aggggcacga agggtcggta cttctcgaat ggcatcttct ttggtgtgat
      421 gttttgggga ggcatatggc tcctatgtcg ggaggcggag acttggaccg ggaaaagaaa
      481 aagcccccgg tcttcggggg cgggcaaaca cgaatacgcg cgtctgccct acatgaggag
      541 aaggagagcg cggttcgatg tcatgttcac ggggcccact gtaggggtcc aatgccccgg
      601 acgcaacggc acgggggagt gggggaggac tggcatctcc tcagaggaga tgccagttgc
      661 gaacgtcgcc tttccccatc aggtcggcca tgacctcgtc gatcgagatc tcgtactgcc
      721 cggtgtcggg cttctggcga cggatgccga agaggcggcg ccgggcgggt gaggtcgtcc
      781 tgcgtgatga gcgcatggct atcccccttc cgggcggttg tcgagcgaat ggcgccgcag
      841 agtctcctgc cagcggcgca ctgcaccaac gcttctcgct acttgggccg aggctccgat
      901 cggttcttga ccgtgattgc tcacgtgggt tcctttctca tatgcgcgcc cgggggcgca
      961 cgtgggtcaa catcgagaac gtaggagtcg gtgctcggat ctcgcttgga tgcgagatgc
     1021 agatcgcttg caggggagtc gggcggggat cgggcgtgcg ggccaatcag ccgtcgggcg
     1081 agagcgccag agtcatgaat gtgctgctgc gactggggtt gtagtcgccg aaggggtcgc
     1141 actcgacaaa accgacccgg gcgtagaggg cccgggacgg ggcgaaggcc tccatcgagc
     1201 ccgtttccag gctcacccga cgtgctccac gctctgcggc cacgctgagt agatggttga
     1261 ccacggtgcg tcccactccg tttccgcgag cttcctcggc ggtatggatg gacttgagct
     1321 ccatatgacc ctcaccgagc tccttgatgg ctccgatgcc cagaagcctg ccatcgtcac
     1381 ggagactgaa gaacgtgacc ttcgggtcga gcaagccgtc gaggtcgagg gcgtgtacat
     1441 cttcgggagg tgtcactccc cgggcaaact ccagatgacg ctgcaacagg gccacgatgt
     1501 cttcccgccg gggatcgtcg atggcaacgg tccaagtttc catcgccgga tgatatgagc
     1561 cggctcaggt tcccgacata ttgacgattg tcgatatgac gacctacatt ggagctccat
     1621 gaaattcggc ctgttcctgc aacccctgca tcacgtctcc gaggatccga cgaaggcgtt
     1681 ggaaagggac ctcgacctca tcgtccactt ggaccagctg gggtactcgg aggcctggat
     1741 cggggagcac cacagcaccg ggtgggagaa catcgccgcg cccgaggtcg tcatcgccgc
     1801 agcggccgag cgcaccgagc agatccgttt cgggaccggg gtcgtgcagg caggtctgca
     1861 ccacccgctg gtggcactcg acaggatgat cctgctcgat catctcaccc ggggccgggt
     1921 cagcttcggg atcggtgttg gtggggggat cccgtccgac ctcgcggtgt tcggtctcag
     1981 tccagagagt gcttctcgcc ggatgcggga gagtctcgat gtgatgctgc ggcttctgga
     2041 gggaaccgag gccgtcaccg agaagacgga ttggttcgag ttgcgagatg cccgactgca
     2101 gcttcggccc tattccacac cgcacatgcc gtttgcggtg gcttctggcg acccggagaa
     2161 cgtggctgtg atggggagga tcggtggcaa ggtccttctc gggggcacac cggaccgggt
     2221 accccaggtg ttggagagtc tcgaaaaggg cgccgcggac gcaggtatcg aggctggaag
     2281 ggaccagatc atgctcagct acgtcatgca tctcgacgag tcacaccgcc aggccctcga
     2341 ggacttcgaa tcgggtgcga tcagagagtt ctacgagttc caggtgg
//
LOCUS       DASUTA010000364         5455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1273600, whole genome shotgun sequence.
ACCESSION   DASUTA010000364 DASUTA010000000
VERSION     DASUTA010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273600"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFZ80_07300"
     CDS             complement(<1..731)
                     /locus_tag="VFZ80_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287275.1"
                     /translation="MSRDGDYSDEMFTKRLSDEDVEAVLSGQAAHDPELAEIARVLSA
                     LDPERFIPETGEKAAEFARKASAVARTGEPAKLAATARFRRGLSLTPRLAPAAVAAIL
                     LFAVSGVAVASNAAVPGDALYGIDRALERIGIGNGGAAERIEEASVLTENGDGAEALE
                     LLAETFDVESSQASDALRKASDRIRENEAANSEVADMLDWMAGEDSRDRQFGQDVAEE
                     ARELGNGEGGEDRGQSGEAPGQDKDT"
     gene            complement(728..1273)
                     /locus_tag="VFZ80_07305"
     CDS             complement(728..1273)
                     /locus_tag="VFZ80_07305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX6287276.1"
                     /translation="MGQPFEEVLVGAKRGDEWAWNQIYHSVSGQLIGYLTMKGAPEPE
                     DQASETLLQVARNIDSFEGDEESFRSWVFVIAHRRLIDSRRRSGRRLETTELEPDLTA
                     ESGDVEQEALASLSTEEVRRMLEILTEEQREVIVLRTLVDLSLEQTADVMGKRVGSIK
                     AMQRRAIARLQKHLDALGVSQ"
     gene            1400..2152
                     /locus_tag="VFZ80_07310"
     CDS             1400..2152
                     /locus_tag="VFZ80_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287277.1"
                     /translation="MKRRLTIMLAVVGMLASAGIAAAAEEDPEDRDDTVFNYGYDEEN
                     HVLLWGAHPTDGLYDCSLEMQGNLIASYGLTEDGLVIVETLHDDDEAVVVFQPTPEGE
                     LSDEFEEPAELPAEYTGPGGECVVGGGVVEGPNGQVNHGMIMKLFNSVYEGPGRGCLV
                     RHLAKSDFGKHDQQINVPDVDTEENGDLSEVDDAEIAFQTALTDCEHGNANKEDKVTG
                     QEKAAAKKAEKANGNRGNSANAPGHNKNNGEG"
     gene            complement(2274..2585)
                     /locus_tag="VFZ80_07315"
     CDS             complement(2274..2585)
                     /locus_tag="VFZ80_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX6287278.1"
                     /translation="METITGDTAEMWGNSIVGFGCYRPKAGGADQEWFEVGFSPRKAA
                     LTLYIMDGFSEYGSLLERLGPHGTGKACLYIKDLSQADTDVLTEIITRSVAAVKEGES
                     I"
     gene            2817..3245
                     /locus_tag="VFZ80_07320"
     CDS             2817..3245
                     /locus_tag="VFZ80_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287279.1"
                     /translation="MDASIDPGILARDLIESADVDWLRDLVDALDREVRVAPLDRLVA
                     LWDLSNASAARLFGVSRQAFAKWLADGPPAGRADDIAAVDNITELLDRYVKRERIPAV
                     VRRPAENLGGASILEIVESGDYSTAAEMVRRAFDLRRVQP"
     gene            3242..3781
                     /locus_tag="VFZ80_07325"
     CDS             3242..3781
                     /locus_tag="VFZ80_07325"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEX6287280.1"
                     /translation="MTPRVKRLSDGHEWLRVADPDWVDPLDPSFAAERGGRWNPPGSH
                     PTLYLNEDIATARSQVEKLLEGSPIGPDDLDPGFDLVVATLPRSQDVADAISDEGLES
                     LELPVTYPRYGNGRPIQHEVCQTVGVMVHDVGLRGVHARSAADGAGLGRELAWFPART
                     TSRASQVSRFRFEDWWYRP"
     gene            complement(3788..4606)
                     /locus_tag="VFZ80_07330"
     CDS             complement(3788..4606)
                     /locus_tag="VFZ80_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX6287281.1"
                     /translation="MLGRLDDRVSLAVAPYMNRPRLVAALVVVVALASLVAFALQIED
                     APRARVYAAEISTTTTSEPDFKALVHSGVISTLPPEEQELTEEALSRATSTTTTIATT
                     TTTTTTTTTAAPSTTTTAASGASTSPPANSTPTTQTTSPPTTSPPTTAASQEGFVGSA
                     ESDFASRINSVRSSDGLGALQRNGSLDSYARDWARHMGRNGSLDHSNIGSLLGAWSGV
                     GENIGFGGSVSVIFDALVGSSGHYQNMVGDWTHMGVGVWRDAQGALWTCQVFAR"
     gene            complement(4635..>5455)
                     /locus_tag="VFZ80_07335"
     CDS             complement(4635..>5455)
                     /locus_tag="VFZ80_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287282.1"
                     /translation="TFLLTLLALAIPAVADEFSSCWVTEVETGFGNTETITRCRIAGG
                     ETVDYASDTDVPTVLYPNVGTDITGQCWYLTSAATAYLVLNRYADGSADIGLDTDPSE
                     PGGIIAIGPTLPRCVSEPTPVADPSADAWNYVMSYIHDPPTPDLNPEPGQGITGLDTF
                     VGVAVPDDHVASLASGVSTLEVEIEVDAVIVSWGDGRTDTYPPSNAILAGYPDGSATH
                     IYEVKNAEGADLTVEYDWTARWRLVGGAWTPLPVPNTTTTVVYPIAEVVSRLTD"
BASE COUNT         1007 a         1638 c         1732 g         1078 t
ORIGIN      
        1 gtgtctttgt cctggcccgg tgcctctcca ctctgacctc gatcttcgcc gccttcgccg
       61 ttgccgagtt ctctcgcctc ttcggcaacg tcctgaccga actggcggtc ccgagagtct
      121 tcacccgcca tccaatcgag catgtcggct acttcggagt tggcagcctc gttctccctg
      181 atccggtcag atgccttacg aagcgcatct gaggcctggc tggactcgac atcgaaggtc
      241 tcggcgagga gctccagggc ctctgctccg tcgccattct cggtgaggac actggcctcc
      301 tcgattcgct cggcagcgcc gccattgcct attccgattc gttccaaagc gcggtcgatg
      361 ccatagagcg catcaccggg aaccgcagcg ttggatgcca ccgccactcc gctcaccgcg
      421 aaaaggagaa ttgcggcaac cgcggctggt gcaagacgcg gtgtcaggga taggccacgc
      481 ctaaagcgcg cggttgccgc aagctttgcg ggttcacctg tccttgccac agcggatgct
      541 ttgcgagcaa actcggctgc cttctctccg gtctcgggta tgaatcgttc cggatcgagg
      601 gcgctcagga cgcgcgcgat ctcggcaagt tctgggtcgt gggctgcttg tccagagagg
      661 acagcctcga cgtcttcgtc cgagaggcgc ttggtgaaca tctcatccga gtaatcgcca
      721 tcgcggctca ttgagatact cccagcgcgt ccagatgctt ctggagacgg gcaatcgccc
      781 ttctctgcat tgcctttatc gatccgactc gtttccccat cacatctgcc gtctgctcca
      841 ggctgaggtc gacgagagtg cgcaacacga tgacttctcg ctgctcttcg gtgaggatct
      901 ccagcatgcg cctgacctct tcggtgctga gcgaggccag agcctcctgc tcgacgtcgc
      961 ccgactccgc ggtcaggtct ggctccaatt cggttgtttc cagtcgtcgt cccgagcgac
     1021 ggcgcgagtc gatcaacctt ctgtgagcga tgacgaacac ccaggagcga aaggactctt
     1081 cgtcaccctc gaacgaatcg atgttgcggg ccacctggag gagggtttca gaagcctggt
     1141 cttcaggttc gggcgcgccc ttcatggtga gatatccgat cagctggccg gacaccgagt
     1201 ggtagatctg attccacgcc cactcgtccc cccttttggc gccgaccagg acttcttcga
     1261 acggttgacc catgtgtgat tgaccgtacc gaggcgtgtt gggatagctc accttcaccc
     1321 gtatccctat cgacatctcg agcgattacg tgagtggttc gtggggtaac cgctgggacg
     1381 aaagcgacca ggagagaatg tgaagagacg actgaccatc atgctggcag tagttgggat
     1441 gctcgccagt gcagggattg cagccgccgc cgaagaagac cccgaagacc gcgatgacac
     1501 cgtcttcaac tacggctacg acgaggagaa ccatgtcctg ttgtggggag cccatcccac
     1561 ggacgggctc tacgactgca gtctcgagat gcaagggaac ctgatcgcca gttatggcct
     1621 caccgaagac ggtctcgtga ttgtggagac gcttcacgat gacgatgagg ctgttgtcgt
     1681 gtttcagccg actcccgagg gagagttgtc agacgagttc gaggaaccgg cggaattgcc
     1741 tgccgagtac accgggcccg gcggagaatg cgtagtcggc ggtggagtgg tcgaaggacc
     1801 caatggccag gtcaaccacg ggatgatcat gaagctgttc aactcggtct acgagggacc
     1861 tggacgcggc tgtctggtac ggcatctcgc caagtccgat tttggcaagc acgatcagca
     1921 gatcaacgtg cctgacgtcg acacggagga aaacggggat ctctcagaag tcgacgatgc
     1981 cgagatcgcg ttccagactg ctcttaccga ctgtgagcac gggaacgcca acaaggaaga
     2041 caaggtgacc ggccaggaga aggcagccgc caagaaggca gagaaagcca acggcaaccg
     2101 cggcaactcg gcaaatgccc ccggtcacaa caagaacaac ggagagggct agcccaaggc
     2161 ccggaggaaa tggatcaggt cacccgccat cgaccgggtg acctgattcg cgtgaagacg
     2221 gccatgggag gctctctcga gcccgctcat tcaggtgtcc tacggccagt cggtcagatc
     2281 gactctcctt ccttcacagc cgccacggac cgtgtgatga tctcagtcaa gacgtcggta
     2341 tcggcttgcg agagatcctt gatgtagaga caggctttac ccgtgccgtg cggacctagt
     2401 cgctccagca gcgacccgta ttcggagaaa ccgtccatga tgtagagggt gagagccgcc
     2461 ttacgtggcg agaagcccac ctcgaaccac tcctggtcgg ctccgcccgc cttcggccgg
     2521 tagcacccga atccgacgat tgaattcccc cacatctcgg ccgtgtcgcc agtgatcgtc
     2581 tccataggtc gcgaacggct atggcgtctc tcttacgggt ttcgttctcg actgtcccga
     2641 ggaactcgtc gacgtcaccg gcgtttcgca ttgtctttcg ctccgccttg tcctcggagt
     2701 caggcggggc tgagaaatct gtcgtggccg ggggagagaa tgtccagatg acgagaacga
     2761 ccttcttcga ttgacatatg gttgcggttg acattcgatt gcgcgataat ggagccatgg
     2821 acgcgtcgat cgatcctggg atcctggctc gagatctcat cgagtctgcc gatgtcgact
     2881 ggctgcgcga cctggtggac gccctcgatc gtgaggtgcg tgtcgcccct ctggatcgac
     2941 tcgtggcact ctgggatctg tccaacgcca gcgccgctcg actcttcggc gtttcgcgtc
     3001 aagccttcgc caagtggctt gccgacgggc cacccgccgg ccgagcggac gatatcgccg
     3061 cggtcgacaa catcaccgag ctgctcgacc gctatgtgaa acgtgaacgc atacccgctg
     3121 tggtgcgtcg tcccgccgag aatctgggcg gcgccagcat tctggaaatc gtcgagtcgg
     3181 gtgactactc gaccgcagcc gagatggtga gacgggcgtt cgaccttcgt cgcgttcaac
     3241 catgaccccg agggtgaagc gactgtccga cggccacgag tggctccggg ttgccgaccc
     3301 cgattgggtg gatcctctcg acccttcctt cgccgccgaa cgcggcggtc gctggaatcc
     3361 gccggggtcc catcccacgc tctacctcaa cgaggacatt gccacggcgc ggtctcaggt
     3421 tgagaagctg ctcgaagggt cgccaattgg gcccgacgat ctggacccag gattcgacct
     3481 cgtcgtggcg acactgcccc gctcccagga cgtggccgac gcaatatcag acgaaggtct
     3541 tgagtccctg gagctccctg tcacctatcc acgctacgga aacggccgac cgattcagca
     3601 tgaggtgtgt cagaccgtcg gtgtgatggt ccatgatgtg gggttgcgtg gtgtgcatgc
     3661 tcgctccgcc gccgacggtg cagggttggg tcgggagctc gcctggttcc cggctcgaac
     3721 gacttcgcgg gcttcgcagg tcagtcggtt tcgattcgag gattggtggt atcggccctg
     3781 atcgccgtca ccgggcgaac acttggcagg tccagagagc accctgggcg tcccgccaca
     3841 cacccacacc catgtgagtc cagtctccaa ccatgttctg gtagtgacct gagctgccga
     3901 ccaacgcatc gaagatcacc gaaaccgagc cgccgaagcc gatgttctcg ccaaccccac
     3961 tccacgcccc gagcagcgag ccgatgttgg agtggtcgag agagccattg cggcccatgt
     4021 gcctcgccca gtcacgggca taagagtcca gtgacccgtt tcgctgcaaa gcacccaggc
     4081 cgtcggagct gcgcaccgag ttgattcggg aagcgaagtc gctctccgcg ctcccgacga
     4141 aaccctcctg agacgctgcc gtggttggag gcgatgtcgt gggaggagaa gtggtttgag
     4201 tggtgggagt ggagttcgca ggcggcgacg tcgaggctcc ggaagctgcg gtcgtcgtgg
     4261 tggacggcgc agccgtggtg gtcgtggtgg tcgtggtggt cgtggtggcg atggtggtcg
     4321 tcgtggatgt ggcccggctg agcgcttcct cggtgagctc ctgctcttct ggaggaaggg
     4381 tggagatgac tccagaatgc acgagcgctt tgaaatcggg ctcagaagtg gtcgtggtcg
     4441 agatctcagc ggcgtagacc ctcgcacgtg gcgcatcctc tatctggaga gcgaacgcta
     4501 cgaggcttgc gagggcgaca acgacgacca gcgcggcgac gaggcggggg cgattcatat
     4561 aaggggcgac ggcgaggcta accctgtcgt ccagccttcc gagcatcgat cgcagatggg
     4621 ttttcgcgac ctcctcagtc ggtcagacga gaaacgacct ctgcgatcgg atagacgaca
     4681 gtcgtcgttg tgttgggaac tggcagggga gtccaagccc cgccgaccag tctccaacgg
     4741 gcggtccagt cgtactccac ggtgagatcg gccccttcgg cgttcttcac ctcgtagata
     4801 tgggtggccg agccgtcggg gtagccggcg aggatggcgt tcgagggtgg gtaggtgtcg
     4861 gtccgtccgt caccccaaga gacgatgacc gcatcgacct cgatctccac ctcgagcgtg
     4921 ctgacgccac tcgccagcga ggcgacatgg tcgtctggca cggccacccc aacgaaagtg
     4981 tcgaggccag ttatgccttg accgggctcg ggattgaggt cgggggtcgg cggatcgtgg
     5041 atgtaggaca tcacgtagtt ccaggcatcg gcggaggggt cggcgacggg ggtcggctct
     5101 gatacgcaac gggggagcgt tggtccgatg gcgatgatcc cgcccggttc gctcgggtcg
     5161 gtgtcgagcc cgatgtctgc gctgccgtcg gcatatcggt tgaggaccag gtaggccgtg
     5221 gcggccgagg tgaggtacca gcattggccg gtgatgtccg taccgacgtt tgggtagagg
     5281 acagtaggca cgtcggtgtc ggatgcgtag tcaacggttt cgccaccggc gatacgacag
     5341 cgggtgattg tctcggtgtt tccgaaccct gtctccactt ctgtcaccca acatgacgaa
     5401 aactcatctg ccacagcggg aatcgcaagt gccaatagcg tcagtaagaa agttg
//
LOCUS       DASUTA010000365         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1662872, whole genome shotgun sequence.
ACCESSION   DASUTA010000365 DASUTA010000000
VERSION     DASUTA010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662872"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VFZ80_07340"
     CDS             complement(<1..638)
                     /locus_tag="VFZ80_07340"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287283.1"
                     /translation="MKRSTLVRGAVFLATVAASWAVLSLGTADPEPDFEVGEPAPQTF
                     YAERDASNVLDEATTEQRRQEAEDEVDPDDARERNQAVETSVSASVDAVFSDVAMLVV
                     GDGAGFAQSPVPELPEPTEGTEGSEGSTTTTEAPDPVLLSGRVFVDLDADGVFDSAAE
                     DAQGDEGLQGVSIGIETYDDSFTVTSGSDGVWTAEVNPGPAVVTLSSRAAIP"
     gene            complement(635..1513)
                     /locus_tag="VFZ80_07345"
     CDS             complement(635..1513)
                     /locus_tag="VFZ80_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005110199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX6287284.1"
                     /translation="MEDLIPEVRFVARGDEVVLTGSPGDVERARTVLDEMLILVQEGQ
                     KLDERRVAEVVKMVTDEVPSPSGVLSDGIPVGRGRMVRPKTHGQQRYMEAIRENTLTF
                     GIGPAGTGKTYLAMAAAVDSLINGSVRRIILTRPAVEAGERLGFLPGDLTAKVDPYLR
                     PLYDALWDMLGPEETASLIDRGTIEIAPLAYMRGRTLNDACVILDEAQNTTPEQMKMF
                     LTRLGFNSKMIITGDISQTDLPTGKHSGLRVVRDILRDIPGVSFVELGARDVVRHRIV
                     ASIVEAYQRFESSESG"
     gene            complement(1693..2100)
                     /locus_tag="VFZ80_07350"
     CDS             complement(1693..2100)
                     /locus_tag="VFZ80_07350"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6287285.1"
                     /translation="MPLVEDFNYALGQRLRAARRHRGWSLGDVESQTEGEFKASVVGA
                     YERGERAISVQRFARLSEIYGTPASELLPAFNEPDLIIDLDAFANDEGDVVERYLAAI
                     QMLRKKPGSDEVRDSDRAVISSLLQSVRATQSD"
     gene            complement(2151..>2414)
                     /locus_tag="VFZ80_07355"
     CDS             complement(2151..>2414)
                     /locus_tag="VFZ80_07355"
                     /inference="COORDINATES: protein motif:HMM:NF016347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX6287286.1"
                     /translation="ATEQSRGAWLMDVVPDLVTLDDLEPGWVVCHPGGERSTPATQTV
                     VIGPEGGFAEEELPDDAAKWDLGPTILRVETAAVVAVAKLSAR"
BASE COUNT          444 a          794 c          715 g          461 t
ORIGIN      
        1 tcgggtattg cggccctact cgacaacgtc accacggcgg gtcccgggtt gacctccgcg
       61 gtccagacac catctgaccc gctcgtcacg gtgaatgagt cgtcataggt ctcgatcccg
      121 atgcttaccc cctgcagccc ttcgtcgcct tgggcatctt cggctgcact gtcgaagacc
      181 ccgtcggcat cgaggtcgac gaacacccgg cccgacaaga ggaccgggtc gggagcctcg
      241 gtggtcgtcg tcgacccttc gcttccttca gtgccctcgg tgggctcggg gagctctggc
      301 accggcgact gtgcgaagcc ggctccgtca ccaacgacca gcatggcgac atcggagaac
      361 accgcatcca ccgaggcact gacggacgtc tcgaccgcct gattccgctc gcgtgcgtca
      421 tcggggtcca cttcgtcctc ggcctcctgc cgacgctgct ccgtggtggc ctcatccagg
      481 acattgctgg cgtcccgctc cgcgtagaaa gtctgaggag caggctcgcc aacttcgaaa
      541 tctggttcgg gatccgcggt gcccagagac aggactgccc aagacgcggc cactgttgcc
      601 agaaagaccg caccacggac cagcgtggaa cgcttcatcc cgactcggag gactcgaacc
      661 gctggtaggc ctcgacgatc gaggcgacga tgcgatggcg aacgacatcg cgggcaccca
      721 actcgacgaa tgagacgccc gggatgtccc ggaggatgtc acgaaccacc ctcaagccgg
      781 agtgcttgcc ggtcggcaag tcggtctggc ttatgtcgcc ggtgatgatc atcttggaat
      841 tgaacccgag tcgagtgaga aacatcttca tttgctcggg cgtcgtgttc tgggcctcgt
      901 cgaggatgac gcatgcatca ttgagagtcc ttcctcgcat ataagcgagc ggagcaatct
      961 cgatagttcc ccgatcgatc agggaggctg tctcctccgg gccgagcatg tcccaaaggg
     1021 cgtcgtagag ggggcgcaga tacgggtcga ctttcgccgt cagatcacca ggtagaaagc
     1081 cgagacgctc acctgcctcg acggcgggtc gagtgagaat gatgcgtcgc accgagccgt
     1141 tgatgaggct gtcgacagcg gcagccatcg ccagataggt cttgccggtt cccgccggcc
     1201 cgatcccgaa tgtgagtgtg ttctcccgga tcgcctccat gtatctctgc tggccatggg
     1261 tcttgggtcg aaccatgcgt ccccgtccca cggggatgcc atcactgaga acacccgatg
     1321 ggctgggcac ctcatcggtc accatcttca ctacctcggc gacacgacgc tcgtccagct
     1381 tctggccttc ttgcaccagg atcagcatct cgtcgagaac ggtcctcgcc cgctccacgt
     1441 ccccgggcga cccggtcagg accacctcat cgccgcgcgc gacaaaacgg acctcgggta
     1501 tgaggtcctc cacctgcctc aggtgtcgat ccgactctcc tatgaggtcg agaagcaggt
     1561 gatgactcgg tacgcgtaac cgcgattcaa tggggtctgg ggacacgggt agacgccttg
     1621 ttcaggcggg tgaatcaacc attctaccga cgtttcgccc tagagccaga atagcttttg
     1681 ggtcccgatt gctcagtcgg actgagtcgc cctcacggac tggagaaggg agctgatcac
     1741 ggcgcggtcc gagtccctga cctcatcact gcctggcttc tttcgcagca tctgaattgc
     1801 cgccagatag cgctcgacaa catcgccctc gtcgttggcg aacgcgtcga ggtcgatgat
     1861 cagatctggt tcgttgaaag cgggtagcag ctcggaggcc ggtgtcccgt atatctctga
     1921 gagacgggcg aatctctgca ccgaaatcgc ccgctcccct cgctcatagg cgcctaccac
     1981 agaagccttg aactcgccct cggtctgtga ctcgacgtca cccagagacc aaccacggtg
     2041 gcgacgcgcg gcacggagcc tctgtccaag cgcgtagtta aagtcttcga ccaacggcat
     2101 ccgctcccat ttcgcctacg tcatcctatt ccccgacgga ggggattgca ctatcttgcc
     2161 gacagtttcg ccaccgcgac cactgccgcc gtctcgacgc gcaggatggt aggaccgaga
     2221 tcccatttgg cggcgtcatc ggggagttct tcctcagcga agccgccctc gggaccgatc
     2281 acgaccgtct gggtagccgg cgtcgaccgt tctcccccag ggtgacagac gacccagccc
     2341 ggctcgagat cgtcgagagt gaccaggtcc ggaacaacgt ccatgagcca ggctccccgt
     2401 gattgctcgg tggc
//
LOCUS       DASUTA010000366         3923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_496131, whole genome shotgun sequence.
ACCESSION   DASUTA010000366 DASUTA010000000
VERSION     DASUTA010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496131"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFZ80_07360"
     CDS             <1..370
                     /locus_tag="VFZ80_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX6287287.1"
                     /translation="AAELVAEALRRLVSLTPQEVSEELAGGTLVVDLREEEELHVVGW
                     IRDSVWAPRGMLEFWADPLDRRHRVEFHPERRLILCCSTGERSALAAATLRTMGYRDV
                     GVLDGGIEAWKQEGLPVEMP"
     gene            367..1326
                     /locus_tag="VFZ80_07365"
     CDS             367..1326
                     /locus_tag="VFZ80_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287288.1"
                     /translation="MRAPPEEVTMRRSVTIVVTVTAMLLSGALAAVAFGGFGDWGAAV
                     DIESYGAGAHEDFNTTSTEGCPFVSPDGKTFFMASTRTGGLGGLDIWVSTRASEDEPW
                     GEPVNVGAPINSADNDFCPTIARDGATFFFASNRPGYCGASVNADIYTTRFGPGLTAG
                     NLIHLSCDVNSGWDEHSPFPAQIPGEGTMLFYSSARPADANDTPGDHDLYMSPRQGGV
                     YMAGIPLSSVNTTANEGQPNVRRDGQELFFWSDRAGGVGGADIYSVQKDSPTGDWGTP
                     QNLGPDVNSAAGETRPSLSWDGTTLYFGSTRVGGQSDVYVTSR"
     gene            1788..2738
                     /locus_tag="VFZ80_07370"
     CDS             1788..2738
                     /locus_tag="VFZ80_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287289.1"
                     /translation="MIEAGVAAFYSGDAERAAELFELADRTDDQIRAESAYQAAIGGR
                     LSLSCTESSAGEFNCLTPYQNAMTDAIGEGGGHDTWPVVVEDGVITRFGFTEHTGMLI
                     EMATFLASEGRFDGYENCVFGPFPESCATIQLENLEDWVEWRQDVEPADHVTVALESW
                     YSGDCEPAVLLAWETDTDCSESSLPAQAIEYESILGAEVSVENCETTSRGDHSNLSCE
                     VHYSNAMSSAVGKPPSVTAREFVLMFGVLTAGANEEPWYDVDYPEDTELRDSFRMYAD
                     EGDLSVEYAAAGCESARTPECANLIIDNLDEWAAWYEANG"
     gene            complement(3142..>3923)
                     /locus_tag="VFZ80_07375"
     CDS             complement(3142..>3923)
                     /locus_tag="VFZ80_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287290.1"
                     /translation="SPGDERVAPEPSTTTLSVAGSAYGLPQGNEPVDLEPAEFTVEID
                     NPYWPMEPGSQWVYREVDEDGTSLEVIVTVTTETKEIANGVTARIVRDTVTEEGELIE
                     DTFDWYAQDSAGAIWYLGEATAEFEDGEITSTSGSFEAGVDGALPGIIMPAEPADGMR
                     YRQEYYQGEAEDNGEILSIEEQAQVPAGHYEDAILTKDSITIEPDVLEYKLYARGVGP
                     VLVFGVSGGGGWEELIEFHQVDASIAEEAGTTPLGEPYLDT"
BASE COUNT          719 a         1175 c         1282 g          747 t
ORIGIN      
        1 cgcggccgaa ctggtggccg aagcgctgcg gcgcctggtc agcctgactc cccaggaggt
       61 gagcgaagag ctggctgggg gaaccctcgt cgtcgatctg cgtgaggaag aagaactcca
      121 cgtggtgggc tggatccgtg attcagtttg ggctccgagg gggatgctcg agttttgggc
      181 cgaccccttg gatcgacgtc accgcgtcga gttccacccc gaacgccgtc tgatcctctg
      241 ctgctcgacg ggggaacgct cggccctggc cgccgccaca ctgcggacca tgggctaccg
      301 ggacgtcggc gtgctcgacg gtgggatcga ggcatggaaa caggaaggac taccggtgga
      361 gatgccatga gggcaccacc agaggaggta accatgagac gcagcgtgac catcgttgtc
      421 acggtgacgg cgatgttgct ctccggggcg ctggccgcag tcgcgttcgg cggttttggg
      481 gattgggggg cggcggtgga catcgagagc tacggagccg gcgcgcacga ggacttcaac
      541 accaccagca ccgagggatg tccattcgtg tcgcccgacg ggaagacatt cttcatggcg
      601 tcgacccgaa ccggcggcct cggcggcctc gacatatggg tgtccacgag ggctagtgaa
      661 gacgagcctt ggggtgaacc ggtcaacgtc ggtgcgccga tcaattcggc ggacaacgac
      721 ttctgcccga ccatcgcaag ggacggggcg acgttcttct tcgccagcaa ccgaccgggg
      781 tattgcggag cgtcggtcaa tgccgacatc tacacgacga gattcggacc cggattgacg
      841 gcggggaacc tcatccatct ctcgtgcgat gtgaacagcg gttgggacga gcacagcccg
      901 ttcccggcac agatcccggg cgaggggacg atgctgttct attcgagcgc aagacccgcc
      961 gacgccaacg acacgcctgg agaccacgat ctctacatgt cgccccggca aggtggggtc
     1021 tacatggccg gtatccctct gtcgagtgtc aacacgaccg ccaacgaggg gcagccgaat
     1081 gtgagacgtg acggccagga gttgttcttc tggagcgacc gggccggtgg tgttgggggc
     1141 gccgacatct attcagttca gaaggactct cccaccgggg attgggggac accgcagaac
     1201 ctcggccccg atgtcaattc cgccgccggt gagacccgac cttcgctgtc ttgggatgga
     1261 accaccttgt acttcggatc gacccgggtc ggggggcagt cggatgtcta cgtgacctcg
     1321 cgctgaatcg aaaacgacgt cgaggagggc tgaccctgct ctcctcgact tcggtgagaa
     1381 ggaacaccag tgaacaagac acagcaacca cagatagcga atgaaccaag ccgaacagag
     1441 gtctggcatg ggaggcagca ggattggtcg ccgcggcagt tgcagtcggc cactggcggg
     1501 tgtgagcgag ggttcgaacg aagcgagccc atgttcgact tcgagagtcg agagccctcg
     1561 cttggtgttt cggagaaagc ggaagttggg ggtacacacc tgatgagccg cctactgagg
     1621 agagcggaga cgagatgaga ggactgagac cggcgctatt ggcagtggca cttgtagcga
     1681 tgactgcatg taacagcggg ggagctgtat caacgacagc gcccactacg accagcgcac
     1741 ctgcgaccag tgcgcccacg accagtgctt cagatggcga tctcgccgtg atcgaagccg
     1801 gcgtggctgc tttctactca ggggacgcgg agcgcgccgc cgagttgttc gagctcgccg
     1861 accgcaccga cgaccagata cgtgcggagt cggcctatca ggcagccatc ggtgggcgat
     1921 tgagcctgag ttgcacggag agctcggccg gggaattcaa ttgcctcacg ccgtaccaga
     1981 acgccatgac ggatgcgatc ggagagggcg gtggccacga cacgtggccg gtcgtggtcg
     2041 aagatggggt gatcacccgg ttcggcttca ccgagcacac ggggatgctg atcgagatgg
     2101 cgaccttcct tgcgagcgaa ggtcgcttcg acggttacga aaactgcgtg tttggtccgt
     2161 tccccgagtc atgcgccacc attcagctgg agaacctcga ggactgggtc gaatggcgcc
     2221 aggacgtcga gccggccgac catgtgacgg tcgcattgga gtcctggtac tcaggggact
     2281 gtgagccagc cgtcctgctg gcgtgggaaa ccgacaccga ttgctcggaa tcgtccttgc
     2341 cggcgcaagc catcgaatac gagtcgatcc tgggtgcaga ggtttcagtt gaaaactgtg
     2401 aaaccacatc tcgcggggat cactcgaatc tctcatgcga ggttcactac tcgaatgcaa
     2461 tgagcagcgc ggtgggcaag ccgccctcgg tgaccgctcg ggagttcgtc ctcatgttcg
     2521 gcgtattgac ggcgggggcg aatgaagagc cctggtatga cgttgactac ccggaggaca
     2581 ccgagctgcg tgacagcttc aggatgtacg ccgatgaggg cgacctgagc gtcgagtatg
     2641 cggccgccgg ttgcgaatcc gcacgaacgc ccgaatgcgc caacctcatc atcgacaacc
     2701 tcgacgaatg ggccgcctgg tacgaggcga acggctgacc gacggagctg atctctcggc
     2761 ttcgacgaac agcgacggcg aaaatagttc ttgctgccct cgacgtgagt gtcaggatac
     2821 ggccaatgcg tcatttctca cgctgcctcg cgctgtctcc cgtggctcgc cccacatcca
     2881 ccccacagac cttcagaacc cctgcgtttc ccctggtcag ggtggtgccc gcatcgttcg
     2941 caacgagggg gtcacgggtt tcaaatcccg tcagctccac catttggtca gggcggtttt
     3001 cggagttccc gcccgtcgct cgagtctcga ttcctcttgc ctcgccctcc acagacggcc
     3061 cacaagacgc tgccgcgagg cacctcgctt attgagaggt gctctcgggt ttgcagtcgc
     3121 cgtgcgcccc gctgtttccg gctacgtatc gaggtagggc tcgccgaggg gagtggttcc
     3181 tgcctcctcg gcgatcgatg cgtcgacctg gtggaactcg attagctctt cccaaccgcc
     3241 tccgccggag acaccgaata ccaggacagg cccgacgccg cgggcgtaca gcttgtactc
     3301 gagaacgtcc ggttcgatgg tgatgctgtc tttggtgagg atggcatcct cgtagtggcc
     3361 ggccgggact tgggcctgct cctcgatgct gagtatctct ccgttgtcct cggcttctcc
     3421 ctggtagtac tcctggcggt atcgcatgcc gtccgccggt tcggccggca tgatgatgcc
     3481 gggcagtgcc ccgtccacgc cggcttcgaa cgaccctgat gtgctggtga tttcgccatc
     3541 ttcgaactcg gcggtggcct cacctaggta ccaaatcgct cccgccgagt cttgggcgta
     3601 ccagtcgaag gtgtcttcga tcaactcgcc ctcttcggtg actgtatctc tcactattcg
     3661 ggcggtgact ccgttggcga tctctttggt ctccgtggtg accgtcacga tgacctccaa
     3721 tgaagtgccg tcttcgtcga cctctcggta cacccactgc gatccgggtt ccatcggcca
     3781 gtaggggttg tcgatctcga ctgtgaactc agcgggctcg agatcgactg gctcgttgcc
     3841 ttgtggcaga ccgtaggccg agccggcaac gctcagagtc gttgtggagg gctccggcgc
     3901 gacacgttcg tcacctgggc tgg
//
LOCUS       DASUTA010000367         2408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1405048, whole genome shotgun sequence.
ACCESSION   DASUTA010000367 DASUTA010000000
VERSION     DASUTA010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405048"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFZ80_07380"
     CDS             complement(<1..242)
                     /locus_tag="VFZ80_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEX6287291.1"
                     /translation="MKTESLNELLYQAMETELGGEQIYLKALECAQNEDLTKEWEKYL
                     EETREHQKILQRVFEAADLDAEVETPGRRIVRHKGEA"
     gene            420..2018
                     /locus_tag="VFZ80_07385"
     CDS             420..2018
                     /locus_tag="VFZ80_07385"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX6287292.1"
                     /translation="MADQVAAPGPMTTVDPKVERRLASAGRMFRWLAVVVAVIAMLGL
                     VYLPLADVPPGPELLRETPRGSAIALGVLSFGALIAGGTSTTVNVAWRRLGLVMCLGA
                     AGFGVFIIYAFLAGLTDIWGPGVAIPAFSVGVILVVLGVAVPLNVSRVDWHVIAGQAC
                     ALLVFSLTMSIFLGYAYGDPSVGRLFLQPPISFQASVISVLLAVGIFLMRPAAGLLST
                     ASSPGAGGRLLRWFGPLVLLTPGLILLITEFVPPTERVDVVAFISVGFGLLLLILLSL
                     FVRALDQTAIEAATAAAQAERARTGLKQEAPVVSGMTEMLHLVDLDDVEGWDVATRYR
                     PGSGAVAGDTSAVQILPDGSVGAVLVDLTGHGADPAVWAIRIRDLLVQSLIAGAAPTQ
                     ALGMVGWAVPEDLLASAIVIKLDPETGWVLLSSAGHPPAIVVSEQEAVMKSPTGPLLY
                     LEHASLYEEHEFELGVGDTLVAFSDGIADVQRTVNARTEPELLADLLLSEGGVATRTA
                     DLVLGFADPEPADDQTVVVLRRER"
     gene            2069..>2408
                     /locus_tag="VFZ80_07390"
     CDS             2069..>2408
                     /locus_tag="VFZ80_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287293.1"
                     /translation="MTEIHDFDDTVIAVLDDPSDAVSDLKAAGYEVEVIKGPEGKDHI
                     DPGGETGGAGSTLKRLLNAFGDQYRVIERLNDELDAGRQVVSVAATPDEGTEAARILQ
                     EHDGDYIWKLG"
BASE COUNT          379 a          742 c          775 g          512 t
ORIGIN      
        1 gcctctcctt tgtggcgcac gatccgtcga cccggggtct cgacctccgc atcgagatcg
       61 gccgcctcga agacacgttg gaggatcttc tggtgttccc gcgtttcctc caggtacttc
      121 tcccattcct tggtcagatc ctcgttctgg gcgcactcca gggccttcag atagatctgc
      181 tcgccaccga gttctgtctc catggcttgg tagaggagct cgttcaggct ctcggttttc
      241 atcgtccctc cttgtcggac tgttgaagcc cgccgcagat acccatgtga cgcttaggcg
      301 aaacaactgc tcagttggag cactggcctg tcgcttccca gacttccgcc agagcagcgg
      361 ccggccagat ttgctagcga ttccctgcgg tgtcagctgc tgtgtaccgt cgccacgaga
      421 tggccgatca ggtggcagcc ccgggaccca tgacgaccgt cgacccaaag gtcgaacgtc
      481 ggctcgcctc cgccggacga atgttccgat ggttggctgt ggtggtagcc gtgatagcga
      541 tgctcggcct cgtgtatctg cccctcgccg acgtgccgcc tggcccggaa ttgctccgcg
      601 agacaccgcg gggctccgct atcgccctcg gcgttctctc gtttggagcg ttgatcgccg
      661 gggggacgtc gacaacggtg aacgtggcat ggcgccggct tggcttggtg atgtgtctcg
      721 gtgcagcggg tttcggtgtc ttcatcatct acgccttctt ggcgggcctt accgacatct
      781 gggggccagg agtggcgatc cccgctttct cggtcggcgt catcctcgtc gtgttgggtg
      841 tggcggtgcc tctcaacgtc agtcgtgtgg attggcacgt catcgccggt caggcgtgcg
      901 ccctgctggt cttctccctc actatgtcga tattcctcgg ctacgcctac ggcgaccctt
      961 cggtgggaag actcttccta cagcccccaa tctcgtttca ggcctcagtg atctcagtgt
     1021 tgttggcggt cggaatcttc ttgatgcgac ctgcggccgg cctgctctcc actgcatcca
     1081 gcccgggagc cgggggccgg ctacttcgtt ggttcggccc cttggtgctg ctcacccctg
     1141 ggctgatcct gctgatcacc gagttcgtcc cacccaccga gcgcgtcgac gtcgtcgcct
     1201 tcatctcagt cggcttcggg ctcttgctcc tgatcctgtt gtcgctcttc gtccgcgctc
     1261 tcgatcagac cgccatcgag gcggccaccg ccgctgcaca ggccgagaga gcccgaaccg
     1321 gtctgaagca ggaggctccg gtcgtcagtg gaatgactga gatgcttcat ctggtcgacc
     1381 tcgatgatgt cgagggatgg gacgtggcaa cccgatatcg tcccggctcc ggggcggtcg
     1441 caggtgacac cagcgctgta cagatactcc cggacgggag cgtgggtgcg gttctcgtcg
     1501 atctgaccgg tcatggagca gaccctgcag tatgggcgat ccgaatacgg gaccttctgg
     1561 tgcaatcgct gattgcgggt gcggccccta cccaggcgtt ggggatggtg gggtgggcgg
     1621 tgcccgaaga cctgttggca tcggcgatcg tgatcaagct cgacccggag actggttggg
     1681 tccttctcag ctccgcaggc catcctccgg cgatcgtggt cagtgagcag gaggccgtca
     1741 tgaagtcgcc aaccgggccc cttctctact tggagcacgc ctcgctctat gaggaacacg
     1801 agttcgagct cggggtgggc gacaccctgg tggccttcag tgacgggatc gccgacgtgc
     1861 agcgcacggt gaatgctcgc accgagccag aactgttggc agacctcctt ctatccgagg
     1921 gcggtgtggc gacgagaacg gccgatctgg tgctcggctt cgccgacccc gaaccggcag
     1981 acgatcagac ggtggtcgtg ttgcggcgcg agcgttgagt ttgtgacggc tcgggggcgg
     2041 gtagttccgc agagaaggag ggccaaccat gactgaaatc cacgatttcg acgacacggt
     2101 catcgccgtc ctcgacgacc cctccgatgc agtatccgat ttgaaagcgg ccggctatga
     2161 ggtggaggtc atcaaaggac ccgaagggaa ggaccatatc gatcccggtg gtgagaccgg
     2221 aggagcgggc tcgaccctga aacgcttgct gaatgcgttc ggagatcagt accgggtcat
     2281 cgagagactg aacgacgagc tcgacgccgg gaggcaggtg gtttcggtgg ctgccacccc
     2341 ggatgagggc accgaagcgg cgagaatcct tcaggagcac gacggcgatt acatctggaa
     2401 gctcggca
//
LOCUS       DASUTA010000368         3823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1015539, whole genome shotgun sequence.
ACCESSION   DASUTA010000368 DASUTA010000000
VERSION     DASUTA010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015539"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VFZ80_07395"
     CDS             complement(<1..673)
                     /locus_tag="VFZ80_07395"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HEX6287294.1"
                     /translation="MTIDALRTEDSRFDSIPDWPYQPRYIEDLPGYQGLRMHYIDEGA
                     SDGHVFLCLHGEPTWVYLYRRMIPIFLGAGARVVAPDLFGFGRSDKPVDDSVYTWDFH
                     RGSLLAFVERMELDDFTLVVQDWGGILGLTLPVEMPEKVSGLLIMNTAFGTGAEPSQG
                     FVEWRDYVAKTPDLAVDRLMKRSCPHLSDAEAAAYEAPFPGPEYKAGVRTFPALVPTS
                     ADMDGV"
     gene            complement(703..1695)
                     /locus_tag="VFZ80_07400"
     CDS             complement(703..1695)
                     /locus_tag="VFZ80_07400"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX6287295.1"
                     /translation="MPDTVVFEDPTGVGKPIELEVFDIPSDLEDAELRIRKVPEPDPH
                     YVDLGMLYRFAQVEAARRSEAEGLVPLHVAVRGHMGTGKDHDIEQFAAAMNLPYFRIP
                     LTGEVRDVTLIGSTHLHGDGKGGTESRWEDGDITRALRGPALLNLSELNAAGAETLFA
                     LHGLLDRYAALDLPNGDTVRLRDDVRMFGTMNPTDIRDYAGTQTLNKAFADRWVIWEK
                     NFPGEEQVRAMLERRYPSLKDVYVEAIARLTVEINASFDATDTGTRVETPLSLRSVLD
                     RLPTGLLIYADGDDPLREAWEEFVLPQVDRYDRDHYETVWNAVVRKGPSEKPFT"
     gene            complement(1688..3772)
                     /locus_tag="VFZ80_07405"
     CDS             complement(1688..3772)
                     /locus_tag="VFZ80_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287296.1"
                     /translation="MRARDQTFLTDLIGPEDQAIEAVVGRYQRVAPAVTRLARSLSDN
                     PNLRVRLGPEASASSDEVVCDPRIFQAAYHRNAPVTPDEVALASALHEVLHLVSTNLE
                     DVRELPADWPVDGQVTDAQVDLLTALERTDRPVAEVLFFSLEDARQESQGLSIYPGAR
                     SVLQDLYLSSLGQAIERSGALSQFVLGCFLLTGGYVEREVLEKRFEARAAVALDDATG
                     HCLEAAESDDPWYVAQLAIELEAIARANGLITEVPETATNAQRKAAEKMDADKAADGV
                     DSVRLVSPIVADAESYQNTKRAADARSGRSARKGASDLADDESTNQLLRVSQAPDVFL
                     PTGQGGKLVVTSIPSAFADFGKEGIDAIADASRRWSVDQRHVSGELFPLFAANQRRGL
                     RSGFDQGDVSPHAALFIGAGLYQRLYERRASRTRRRYAVSLLVDGSASMLSPGRSSRT
                     GTQWAMPAALLGAWSMARLCDELQIDFEVAVFNRGFAARPADTEETYVKARSAATAGL
                     KRTQGSAADRLTSTVNHYMVKPFDRRWRDSQHTLAGLFFTAMSPRRASTQARRDTKSA
                     PPVSLFEKASNVDEFNVMHAAERMGRLGADVRLLMVLADGMTRGSVEALTKTVQSVED
                     SGTTVIGIGIGDHTVDQAYQRHEVVDEPDQLTTAMVEGTRSALRRSLAYSGMDTWWLR
                     ASGFQHKEQSVA"
BASE COUNT          656 a         1195 c         1258 g          714 t
ORIGIN      
        1 cgacaccatc catgtccgcc gaggtcggga ccagagccgg gaaggtgcga acccccgcct
       61 tgtactcagg gccggggaag ggtgcttcgt aggcggcggc ttcggcatcg ctgagatggg
      121 ggcacgaccg tttcatcagg cgatccacgg ccagatcggg ggtcttggcc acgtagtccc
      181 tccattcgac gaaaccctgg gacggttccg ctcccgtgcc gaaagcggtg ttcatgatga
      241 ggaggccgga caccttctca ggcatctcca cgggcagggt cagccccagg atgccacccc
      301 agtcctggac caccagggtg aagtcatcga gctccatgcg ctcgacgaag gccaggagcg
      361 agccacgatg gaagtcccat gtatagacgg agtcatcgac tggtttgtcg gagcgcccga
      421 atccgaagag atccggtgcc acgacacgag ctcccgcccc caggaagatc gggatcatcc
      481 tccgatacag atagacccat gtcggctcgc catgaaggca caggaaaacg tgcccgtcgc
      541 tcgccccctc gtcgatgtag tgcatgcgca gcccttggta gcccggcaag tcctcgatgt
      601 atctcggctg gtacggccag tccggtatcg aatcgaacct ggagtcctcg gtgcgcagtg
      661 cgtcaatggt catcggccga ggctatccca acctctcccg agtcaggtga atggcttctc
      721 ggacggcccc ttgcggacga cggcgttcca cacggtctca tagtggtccc ggtcgtaacg
      781 gtcgacctgg ggcaggacga actcctccca ggcctcacgt agtggatcgt cgccgtcggc
      841 atagatgagc aagccggtgg gcagccggtc gagaaccgaa cggagggaga gcggtgtctc
      901 cactcgggtc ccggtgtccg tggcgtcgaa tgatgcgttg atctcgaccg tcaggcgggc
      961 aatggcctcg acatagacgt ccttgaggct ggggtagcgc ctttccagca tggccctcac
     1021 ctgttcttcg ccggggaagt tcttctccca gatgacccag cggtcggcga atgccttgtt
     1081 gagggtctgg gtgccggcat agtcccttat gtcggtgggg ttcatggtcc cgaacatgcg
     1141 gacatcgtca cggagtcgaa cggtatcccc gttagggagg tccagggcgg cgtagcggtc
     1201 gagcaggcca tggagagcga acagggtctc ggcaccggcg gcgttcagct cggacaggtt
     1261 gagcagtgct gggcctctta gcgcccgggt gatgtccccg tcttcccatc ggctctccgt
     1321 gcctccttta ccgtctccat gcaggtgggt cgacccgatc agggtcacat cccgtacctc
     1381 cccggtgaga ggaatccgga agtaggggag gttcattgct gcggcgaact gttcgatgtc
     1441 gtggtccttg ccggttccca tgtggcctct gaccgcgacg tggagtggga cgaggccctc
     1501 tgcctcgctc cgtcgcgccg cctcgacctg ggcgaaccga taaagcatcc cgaggtcgac
     1561 gtaatgcggg tcgggttcgg ggaccttgcg gattcgaagc tcggcgtctt cgaggtccga
     1621 tgggatatcg aagacctcga gctcgatggg tttccccacg ccggtcgggt cttcgaaaac
     1681 gactgtgtca ggcaactgat tgctccttgt gttgaaatcc cgatgctcgc agccaccagg
     1741 tgtccattcc cgagtaggcg agactcctgc gcagggcact ccgggtgccc tcgaccatgg
     1801 ctgtggtcaa ctgatcaggc tcatcaacga cttcatgacg ttgatatgcc tggtccaccg
     1861 tgtgatcgcc gatgccgatc ccgatgacgg tggtcccgga gtcttcgacc gattgaaccg
     1921 tcttggtcag ggcttccaca gagcctctgg tcataccgtc ggcgaggacc atgagtaacc
     1981 gcacgtctgc gccgagccgt cccatccgct cggcggcatg catcacgttg aactcgtcga
     2041 cattcgacgc cttctcgaac agggacaccg gtggagccga cttcgtgtcg cggcgggcct
     2101 gagtcgaggc gcgccgtggg ctcatcgcgg tgaagaacag gccggcgagc gtgtgttggg
     2161 aatctcgcca gcggcgatcg aacggtttga ccatgtagtg gttgaccgtg gaggtgaggc
     2221 ggtccgccgc agagccctgt gttctcttca gcccagccgt ggctgccgac ctcgctttca
     2281 cgtaggtctc ctcggtgtca gcgggtctgg cggcgaatcc ccggttgaac acggcgacct
     2341 cgaaatcgat ctggagctca tcacagagac gggccatgct ccacgccccg agtagggctg
     2401 ccggcatggc ccattgcgtc ccggtgcgag agctacgacc cggactgagc atcgaagcgg
     2461 agccgtcgac caggagtgag accgcatagc ggcgcctggt ccgcgaagcg cggcgctcat
     2521 aaagacgttg gtacaagccg gcaccgatga acagagctgc gtgtggtgag acgtcgccct
     2581 gatcgaaccc ggagcgcaga cctcgccgct ggttggcggc gaacagcggg aacagctctc
     2641 cggagacgtg acgctggtcg acggaccacc gtctcgaggc gtcggcgatg gcgtcgatgc
     2701 cttctttccc gaagtcggcg aaagccgacg ggatcgaggt gaccacgagc ttgccgccct
     2761 gaccggtggg gaggaagacg tccggcgcct ggctgacacg caacagctga ttggtcgact
     2821 cgtcatcggc caggtccgag gcacccttcc gggccgaccg gcccgacctg gcgtctgcgg
     2881 cgcgcttggt gttctggtac gactcggcgt cggcgacgat cggtgagacc aatctcacgc
     2941 tgtccacacc gtcggcagcc ttgtcggcat ccatcttctc agctgctttc cgctgagcgt
     3001 tggtcgcggt ctcaggaacc tcggtgatca atccattcgc cctggcgatc gcttccagct
     3061 cgatggccag ctgagcgacg taccacgggt cgtcggattc ggccgcttcc aggcaatggc
     3121 cggtggcgtc gtccagggcc actgcagccc ttgcctcgaa tcgcttctcg aggacctccc
     3181 gctcgacgta cccgcccgtc aggaggaaac acccgaggac gaactggctc agggcgccgg
     3241 agcgttcgat cgcctggccc agagatgaca ggtagaggtc ctggagcacc gagcgcgctc
     3301 cgggatatat cgacagcccc tgtgactcct gacgggcgtc ctcgagtgag aagaagagca
     3361 cctcggcgac cggcctgtcg gttcgctcca gcgcggtgag taggtcgacc tgagcgtcgg
     3421 ttacctgacc gtcgaccggc cagtccgcag gcagctcgcg cacgtcctcg agattggtcg
     3481 agaccaggtg caggacctcg tgcagtgcgg aggcgagggc gacttcgtcg ggagtgacgg
     3541 gggcgttgcg gtggtacgcc gcttggaaga tgcgcgggtc acaaacgacc tcatcggagg
     3601 atgcagacgc ctcgggcccg agtcgcactc tcaggttcgg gttgtcggag agactccggg
     3661 ccagtcgggt gaccgccggc gcgacgcgtt ggtaacgacc gaccacggct tcgatggcct
     3721 ggtcctcggg accgatcagg tcggtgagga atgtctgatc gcgtgcgcgc acagtcgttc
     3781 attgtctcat gccttgtcaa gcctccccaa ccgacttccc tct
//
LOCUS       DASUTA010000369         2743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1923857, whole genome shotgun sequence.
ACCESSION   DASUTA010000369 DASUTA010000000
VERSION     DASUTA010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923857"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2743)
                     /gene="gltB"
                     /locus_tag="VFZ80_07410"
     CDS             complement(<1..>2743)
                     /gene="gltB"
                     /locus_tag="VFZ80_07410"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX6287297.1"
                     /translation="LIPAAWENVTDLSPELSAFYEYHAFLTEPWDGPAAIAVTDGVSL
                     LAGMDRNGLRPARWTLTPDVVIVASEVGVCPEEEAHALETGQLGPGELIFFDGVTGEV
                     ARSDDVKERLARALPYSDWVSTEALHIQAPFDSLNDDRFDAGALTRVFGYTAEERRLI
                     LAPLAQGTTPTGSMGDDTGLAVLAERPRRLTRYFHQMFAQVTNPPMDPIREKLVMSLR
                     IQLGRRGSLLEDSPSRAHLIELASPILSDEELNSIVRTGDRRFFSHWIAATWEASDGP
                     DGMKQRIDEICTESADAVRLGASILVLSDRETSAAEAPIPILLIVGAVHHHLIDEGVR
                     GDVSLVVVSGEPRDAHDIAALIGFGASAVNPYLAIDQVIDLARSGMIDIDPVTAQENY
                     RSGLETGLLKIMSKMGICTLSAYRGSELFEVMGLSEGVCHRAFRNAPRRLRGVGFSHL
                     ANQALRAHADYRSGEPMPGGFYKHRRGEEFHITGPKIVLDLQKSVRSGETEAWGKYLN
                     TIGDRSPALVRDLLTFVDVDPIPIEEVEPAERIMRRFVTAAMSLGALSKEAHETLAEA
                     MNSIGGLSNSGEGGEDPGRFGTPRNSGIKQVASARFGVTPAYLSSAEELQIKMAQGSK
                     PGEGGQLPGHKVSSEIARLRHTEPGVTLISPPPHHDIYSIEDLAQLVYDLKSFKPTVR
                     VSVKLVSEPGVGTIAVGVAKADADVITVSGSEGGTGASPLVSIKHAGSPWELGVAEAH
                     QALVSKGMRAKVILETDGGLRTGRDVIVAALLGAERFGFGTLPLLALGCKMVRQCHLN
                     TCPVGIATQDLELRAKYAGSVDQVIILFRHLAEEIRHHLAALGAGTLDEIIGRADLLR
                     PIDSGHPLASDLAAMLISAQGRRRHEGYTPHPYSRLSERIAADTRKAVETGEPVELI"
BASE COUNT          493 a          852 c          925 g          473 t
ORIGIN      
        1 taaatgagct cgacgggctc accggtctcg accgcctttc gagtgtcggc ggcaatccgc
       61 tcactgagac gggagtaggg atggggggtg tacccctcgt gacgtcgtcg gccttgggca
      121 ctgatcagca tggctgcgag gtcgctggcg agcgggtgtc cggagtcgat cggccgaagg
      181 agatcggccc gcccgattat ctcgtcgaga gtgccggccc ccagggctgc gagatggtga
      241 cggatctcct cggcgagatg ccggaacagg atgatcacct gatccaccga gcctgcgtac
      301 ttggcgcgca actcgaggtc ctgtgtggcg atacccaccg ggcaggtgtt gaggtggcat
      361 tgacggacca tcttgcagcc gagagcgagc agaggaagag tgccgaagcc gaatcgctcc
      421 gcaccgagaa gggcggcgac gatcacgtcg cggcccgtcc ggagaccccc atccgtctcc
      481 aggatcacct tggcgcgcat gcccttggag acgagggcct gatgagcctc ggccaccccc
      541 aactcccaag gagatccggc gtgcttgatc gagacgagcg gtgatgcgcc ggtcccgccc
      601 tccgagccgg acaccgtgat gacgtcggca tcggccttgg ccactcccac ggcgatggtg
      661 ccaacgcccg gctcgctgac cagcttgaca gagacccgga cggtcggctt gaacgacttg
      721 aggtcgtata cgagctgggc gagatcctcg atcgagtaga tgtcgtggtg aggcggtggc
      781 gagatgaggg tgacgccggg ctcggtatgc cggagccgtg cgatctcgga cgacaccttg
      841 tgacccggaa gctggccccc ctccccgggt ttcgagccct gagccatctt gatctgcagc
      901 tcctcggccg agctgagata ggccggagtc acaccgaatc gcgctgatgc gacctgcttg
      961 atgccggagt tcctcggcgt tccgaaccgt ccaggatcct cgccgccttc gcccgagttg
     1021 gacaggccac cgatcgagtt cattgcctct gccagagtct cgtgggcttc tttggagagg
     1081 gcaccaagcg acatggcggc tgtcacgaag cggcgcatga tccgttcggc gggctcgact
     1141 tcttcgatcg ggattggatc gacgtcgacg aaggtcagca gatcccgaac cagggcgggc
     1201 gacctgtcgc cgatggtgtt cagatacttg ccccaggcct cggtctcccc cgagcggacc
     1261 gacttctgca gatcgagaac gatcttgggc ccggtgatgt ggaactcctc accgcgtcga
     1321 tgcttgtaga agccaccggg catgggctca ccggagcggt agtcagcgtg cgcccggagg
     1381 gcctgattgg ccaggtggct gaacccgaca ccgcggagac gtcgtggagc gttgcggaaa
     1441 gcgcgatggc agacaccctc ggaaagaccc atcacctcga agagctccga cccgcgataa
     1501 gcggagagag tgcaaatgcc catcttggac atgatcttga ggagaccggt ctcgaggccg
     1561 gagcggtagt tctcctgggc ggtcaccggg tcgatgtcga tcattcccga ccgtgccaga
     1621 tcgatcactt gatcgatcgc caggtaaggg ttgaccgcgg atgctccgaa gccgatgagg
     1681 gcggcgatgt cgtgggcgtc tctcggctcg ccggagacga cgaccaggga gacatccccc
     1741 cggacgcctt cgtcgatcag gtgatgatgc acggcgccga cgatgaggag gatcggtatc
     1801 ggagcctcgg cggcactcgt ctcccgatcg gagagcacca atatcgaagc accgagccgc
     1861 accgcatctg cggactcggt gcagatctcg tcgatccgct gtttcattcc atcagggccg
     1921 tcactcgcct cccaagtggc ggcgatccaa tgagagaaga agcgacggtc tccggtgcgg
     1981 acgatcgagt tgagctcctc gtcggagagg atggggcttg ccaactcgat caaatgcgcc
     2041 cggctgggcg agtcctcgag cagcgagccg cggcgaccca gctggatgcg cagcgacatg
     2101 accagcttct ccctgatggg gtccatcggc ggattggtca cttgggcgaa catctggtgg
     2161 aagtagcggg tcaggcgtct cggacgctcg gcgaggaccg ccagtccggt gtcgtcaccc
     2221 atcgagccgg tcggggtcgt cccctgcgcc agtggagcga gaatcagccg gcgctcctcg
     2281 gctgtgtaac cgaacacccg ggtcagagcg cccgcatcga accgatcgtc gttgagggag
     2341 tcgaacggtg cctgtatgtg cagcgcctcg gtgctcaccc aatccgaata tgggagtgct
     2401 cgggcgagtc tctccttcac gtcatcggag cgtgctactt caccggtaac gccgtcgaag
     2461 aagatgagct ctcccggccc cagctgtcct gtctccaacg catgcgcttc ctcctcgggg
     2521 cacaccccga cctctgaggc gacgatgacg acgtcggggg tgagagtcca ccgagccgga
     2581 cggaggccgt tgcggtccat gcccgcaagg agggagacac cgtcggtgac ggcgatggcc
     2641 gccgggccgt cccacggctc ggtcaggaat gcgtggtact cgtagaaggc cgacagctcg
     2701 ggagagagat cggtgacgtt ctcccacgcc gccgggatga gca
//
LOCUS       DASUTA010000370         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1794429, whole genome shotgun sequence.
ACCESSION   DASUTA010000370 DASUTA010000000
VERSION     DASUTA010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1794429"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..1025
                     /locus_tag="VFZ80_07415"
     CDS             57..1025
                     /locus_tag="VFZ80_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6287298.1"
                     /translation="MQRVSIYLAVLGLVATGCSDLEPISESVTEVIEIESVDLPGTLW
                     EPLMPPLSAGSAVTVEGTLTLPPTDVPVPAVILVHGCGGVGGSERGWVPDLRDEGYAS
                     LLLDSFGGRGLTEICTGAETLNVASPIVDLFRATEVLRDHPYIDGNRIGVIGFSFGGR
                     TALWSAMTRFREAYEGAELAVHVAFYPSTCFIRLQGETEVSEAPIRILHGSEDDWTPI
                     DQCQEFIARMSSSGADAELIAYPGAHHGFDNETLPPSGTVPLEAVSPRNCQFEEIDGS
                     IIDPDTGAVAGVGSPCVEVGVQVGYNRDAHMAAEADLFQLLAEVFG"
     gene            1684..>2031
                     /locus_tag="VFZ80_07420"
     CDS             1684..>2031
                     /locus_tag="VFZ80_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287299.1"
                     /translation="MPMTEAHKAALAEGRKQARAIKSYLKAIDSRKPGRPVTKEGLES
                     RLARIAEKLEQSDNPLDEIDLIQSRLDIESALADLEDARDLEDLEAGFIEHAAAYSDR
                     KGISYTAWREYGVP"
BASE COUNT          382 a          612 c          645 g          392 t
ORIGIN      
        1 gaatcaagcc ttctggtggg caaggcgtct tggcggccta tcgctaaatt cctgcgatgc
       61 agcgcgtctc gatttatctg gcggtgttgg ggctcgtagc cacgggatgc tcggacttgg
      121 agcccatctc ggagtcggtg accgaggtga tcgagatcga gagtgtcgat cttcccggga
      181 cactatggga gccattgatg ccaccgctct ccgcagggtc cgcggtaacc gtggaaggaa
      241 ccctgaccct gcctcccacc gatgtgcctg tgcccgcggt gatactcgtc cacggatgtg
      301 gcggcgtcgg tggttctgaa cggggctggg tcccggatct tcgggacgag ggctatgcga
      361 gtctcctcct cgacagtttc ggtggcaggg gcctaacgga gatctgcacc ggagcggaga
      421 ctctcaacgt cgcctcgccc atcgtggacc tctttcgagc caccgaggtt ctcagagatc
      481 atccctacat cgacggcaat cgcatcggag tcatcggttt ctcgtttggt gggcggacgg
      541 cgctgtggtc ggcgatgacc cgttttcgag aggcatacga aggggccgag ctggcggtac
      601 acgtcgcctt ctatccgtcg acctgcttca ttcgtcttca gggtgagaca gaggtgagtg
      661 aggcacctat ccggatcctg catgggagtg aagacgactg gacgccgatt gatcaatgtc
      721 aggagttcat cgccaggatg tcatcgtcgg gcgccgacgc agagctgatc gcgtatccgg
      781 gggcgcacca cggcttcgac aacgagactc ttcctccatc ggggacggtg cccctcgagg
      841 cggtgagccc ccgcaattgt cagttcgagg agatcgacgg cagcatcatc gaccccgaca
      901 cgggcgcggt cgccggggtc ggttcgccgt gtgtcgaggt gggagtccag gtcggataca
      961 acagggacgc acacatggcg gccgaggcgg atctgttcca gctcttggcg gaggtcttcg
     1021 gttgagctat cccggcgaaa tctcttctac ccgatcctga tcccgacgca gctcagagcc
     1081 cggaaacccc ctcttgccgg gttcccgggg tctgacgtcg accgaagacc gtcgagttcc
     1141 ggctcaccca gcgcgaggcg gattcggcgc ccatcggttc gtctcaggag tggaagccag
     1201 tcttcttctg ccgccagcat ctcggtggca tgtgctgagc agagcctgcc ataaggcgtg
     1261 atggcgacag gaccactccc acagcgccca cagctgtcga gagaggagga agaaaggtct
     1321 gccggggagt ggtccttcat cgcatccctg acatcgtcaa ccaggaccgt tggcttgggt
     1381 gtcattaccc aaacccccag atggactagt catttgtcgc gaaatcgggc agaatgttgg
     1441 gaaccgaggt caggttttca atccaggaac agttgtcacg ggcgggcccg atttccgcca
     1501 atcacggggc aagtggttct agtttgaagg cttccgggcg actctggatt gcccccacgt
     1561 cgctcggtgt gacgggccaa tcaaggggct ccgcgactct cgccccccaa tcccaatcgc
     1621 ggagccctgg cccgttttgg tcacccgccg acatgaccag ctcatgtgtg tcatgatccg
     1681 ccgatgccaa tgaccgaagc gcacaaggcc gcactggctg agggccggaa gcaagcacgg
     1741 gccatcaagt cgtacctgaa ggcgatcgac tccagaaagc ctgggcggcc cgtgaccaag
     1801 gagggcctcg agtcccgtct cgccaggatt gcagagaaac tcgagcaatc agacaaccct
     1861 ctggacgaga tcgacctgat tcaatcgcgt ctcgacatcg agtctgctct ggcagatctc
     1921 gaggatgccc gagacctcga agacctcgaa gcgggtttca tcgagcatgc cgccgcgtac
     1981 tcggaccgga aagggatcag ctacacggca tggcgcgagt acggggtccc c
//
LOCUS       DASUTA010000371         3691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_628171, whole genome shotgun sequence.
ACCESSION   DASUTA010000371 DASUTA010000000
VERSION     DASUTA010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628171"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(572..1681)
                     /locus_tag="VFZ80_07425"
     CDS             complement(572..1681)
                     /locus_tag="VFZ80_07425"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017200318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6287300.1"
                     /translation="MSYGAPERLNTLVIGGGQAGLAVGYLLTQAGVPFLIVDANQRTG
                     DAWRNRWDSLRLFTPNRYNSLPGMTIPGEDWGFPTKDELADYLESYAEHFELPIRHGV
                     RVDHLTRDGNCFLATSGDMSFESDNVIVAMASYQVPKRPGFANELDSRIVQLHVDDYK
                     NPGQLEEGDVLVVGLGNSGAEIAKELAKGRRVWLSGKPSAVQPFRPERLSGRILMPFV
                     GPVILNRVLTSSTPMGRRFHSKMRHRAMPLVRVKPKDLVAAGVQRVGRTTGVVDGYPQ
                     LEDGTVLEVPNVVWSTGFAPGFSWIDLPVFDEAGDVIHNRGVVDQVPGLYFTSLKFLY
                     SVLSDTLFAIRRDAGYVVDHLVERQQENERSQLGS"
     gene            complement(1771..3333)
                     /locus_tag="VFZ80_07430"
     CDS             complement(1771..3333)
                     /locus_tag="VFZ80_07430"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX6287301.1"
                     /translation="MRETVPKTTEEVRAAEVIASLCLATDLGMGIPFEHGLHATLVTM
                     RLCKALNADQETLSRTYYASLLMYAGCTVDGDVRSEIFGGSLTEHHAHRQFGSRVESL
                     SGVVGALPSPDANLPHRAWEVVTRLPRAARFMSGHFAPLCEVAGMLAERLGMPKSIHE
                     MFPVLTERWDGKSVLKRASGDEIPLPLRIIHVGRDAAYQRLIGDDAYVKEVIRSRSGK
                     AFDPDVVSIFLDNADEVLGPTEPPTTAWEDVLDAEPRPPMMLHGPAIDRALRAMGAFS
                     DLVSPYLSGHSSGVADLAATAAELHGFADDEVKTIRRAGFVHDLGRTAVSSRVWNKPG
                     SLTADEREQARLHPYHTERVLSRSPFLAEIGSIAMAHHERLDGSGYHRALDVTSLSPA
                     SRLLAAADAFQSKIEPRPYRKALSPEEATSILADKAKQGGFDPAMVTAIAEAAGQKPP
                     TIERPDGLTERELEVLGLLARGHQTKQIARHLEISPKTVDRHIQNSYRKMGVSSRAAA
                     TLFAAENGLVSR"
BASE COUNT          696 a         1179 c         1131 g          685 t
ORIGIN      
        1 actgccgcag atccagtgcg tacgccccga gcgtcttacc ggaatagccg gccaagaatc
       61 cgcccatcgc cacccgaagc ccgaaacggg tcaaagcccg cctccaccac catgggcacc
      121 aacccgtaat ctgagttgaa catcgcatac tttccttcat ccccatcgga cgaaggaccg
      181 gacctgcaca gccggtgtca gctcaagtca acacctaacc gagcacgagg tgccgatatc
      241 ggtcggtccc aaatgtgggc ggtttcgcac gctatcgagt gatctgtgcg gcccattctg
      301 tccattggaa cctcatcccg accactgacc tcgaagaatc caccgacgcc gtcggctatc
      361 aaacaccgac acttggttcg ctgtacatcg tcttgggtcg atgtgtgact ggctggtgcg
      421 ccaaggaaca gcctcggctg tcggcccaca tccacccaga tgaggccgca tcaaatgcag
      481 ttgccgccaa gctcgatctc gatccgacgg cgagggcgag atgatctgga agtagcagtg
      541 gcttgagcag ctcatgcccc acaccttcgc ttcaggaccc cagctgagac cgctcgttct
      601 cctgttgccg ctcgacgagg tgatcgacta cgtagccagc gtctcggcgg atggcgaaca
      661 acgtgtctga cagcaccgag tagaggaact tcaaactcgt gaagtagaga ccgggcacct
      721 ggtcgacgac gccccgatta tggatgacgt cacctgcctc atcgaacacg gggagatcga
      781 tccaagagaa gccgggagcg aagccggtgc tccaaaccac gttgggtacc tcgagaactg
      841 tcccatcctc caactggggg tagccgtcga cgacacccgt tgttcgaccc actcgctgaa
      901 ctccggcggc gaccaggtcc ttcggcttga ctcgaaccag gggcatggct ctgtggcgca
      961 tcttggagtg gaaccttctg cccatcggcg tgctgctggt gagcacgcgg ttgaggatca
     1021 ccggcccgac gaagggcatg aggatcctcc cggagagacg ttccggtcgg aagggctgga
     1081 ctgcgctggg tttgcccgag agccagaccc gtcgcccctt ggccaactcc ttggcgattt
     1141 cggcgcctga gttgcccaga ccaacgacga gaacatcgcc ttcctcgagc tggcccgggt
     1201 tcttgtagtc atcgacatgg agctggacga tccgcgagtc cagttcattg gcgaagccag
     1261 gtctcttcgg cacctggtat gacgccatgg ccacgatcac attgtcggat tcgaaagaca
     1321 tgtcaccgga tgtggccaag aaacaattgc cgtcgcgggt gagatggtcc accctcaccc
     1381 catgacggat gggcagctcg aagtgctcgg cgtacgactc gaggtagtcg gcgagctcgt
     1441 ctttggtggg gaagccccaa tcctcaccgg ggatggtcat gcccggcagc gagttgtatc
     1501 ggttgggggt gaacaagcgc agggagtccc atcggtttct ccaggcgtca ccagtcctct
     1561 ggttggcgtc gacaatgagg aaaggcaccc ccgcctgggt taggagatac cccacggcca
     1621 aaccggcctg acctcccccg atgaccaaag tgttcagtcg ctctggtgct ccgtagctca
     1681 tgtcactacc tccatgcctc gtcgcatgtc tcgaaggtaa ggacagagcc ccggactgtg
     1741 aatacggaat actccccata tgggagtggc ctatctagag accaagccgt tctcggcggc
     1801 gaacagggtg gcggccgccc gcgacgacac ccccatcttg cgatagctgt tctggatgtg
     1861 acggtcgact gtcttcgggg agatttcgag atgacgggcg atctgcttgg tctggtgtcc
     1921 ccgggccagc aaaccaagca cttccagttc gcgctcggtc aaaccgtctg ggcgctcgat
     1981 cgtgggcggc ttctgcccgg cggcctcggc gattgccgtg accattgccg ggtcgaatcc
     2041 gccctgtttg gctttgtcgg ccagaatcga ggtcgcctcc tccggagata gtgctttccg
     2101 ataagggcgt ggctcgatct tggactggaa ggcgtccgcc gccgccagca gccgggaggc
     2161 tggcgacagg gaagtgacgt cgagagcccg gtggtagccg gacccatcca aacgctcatg
     2221 gtgggccatg gcgatcgagc cgatctcggc gagaaacggt gaccgggaca gcacgcgctc
     2281 ggtgtgatag gggtgaagcc ttgcttgctc gcgctcgtcg gccgtcagag atcccggctt
     2341 gttccagact cgcgatgaga cggccgtcct cccgaggtcg tgcacgaaac cggcgcgccg
     2401 aatcgtcttc acctcatcgt cggcgaaccc gtgcagctcc gccgcagtcg cagccaggtc
     2461 ggctacgccc gaggagtgac cggagaggta gggggagacc aggtctgaga acgcacccat
     2521 cgccctcaaa gcccggtcga ttgctggccc atgcagcatc atcggcggcc ggggctcggc
     2581 gtcgaggacg tcctcccacg cggtggtcgg gggttcggtc ggccccagca cctcgtcggc
     2641 gttgtccagg aatatggaca ccacatctgg gtcgaaagcc tttccagatc tggaccgaat
     2701 cacctccttc acgtaggcgt cgtcaccgat cagtcgctgg tatgcggcgt cccggcccac
     2761 gtggatgatg cgcaacggca gcgggatctc atcgccactg gcgcgtttga ggacgctctt
     2821 gccgtcccaa cgctcggtga gcaccgggaa catctcgtga atcgacttcg gcatcccgag
     2881 acgctcggca agcattcccg cgacctcgca caacggggcg aaatgcccgc tcatgaaccg
     2941 ggctgcccga gggagcctgg tgaccacctc ccacgctcgg tgagggaggt tcgcatcggg
     3001 ggatggcaaa gctccgacca ccccggagag actctcgacc cgagagccga actgccggtg
     3061 cgcgtggtgc tcggtcaggc tgcccccgaa gatctccgat cgcacgtccc cgtcgacagt
     3121 gcagccggca tacatgagca gggaggcgta ataggtgcgc gacagcgtct cctggtcggc
     3181 gttgagggcc ttgcagagcc gcatcgtgac caacgtggca tggagcccat gctcgaaagg
     3241 gatccccatc ccgagatcgg tggccagaca caacgaagca atcacctcgg cggcccgaac
     3301 ctcctcggtg gtcttcggta cggtctccct cacctggaga acagtagttc ggttgccccc
     3361 accggccggc ggcccacgcg ataatgaagt gccgtactgc tcggctggcg gaagcgtgcg
     3421 ttatcgctcg gtaatggtga tcagcatggc tggatttgcc agacctctgc tggctgggct
     3481 caacaaggag cggtcgaaac cagaacaaga cgggccccag gtcggacggc gacccaccta
     3541 gtggttactc ccggctgcct tcttcggagt ctcgtgaccg ttagccggac gtctgccgat
     3601 tctcagctct gaccgatgcc gtggagttcc ttgtcggtcg cgatcccatc aaccctcatt
     3661 gtctccctgc tctccacgct ggtctctctg g
//
LOCUS       DASUTA010000372         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1664140, whole genome shotgun sequence.
ACCESSION   DASUTA010000372 DASUTA010000000
VERSION     DASUTA010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664140"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VFZ80_07435"
     CDS             <1..856
                     /locus_tag="VFZ80_07435"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6287302.1"
                     /translation="LLAANSVSQTSRIVFNLLGTGAAGVLAAVAPTLAPAFIIDSATF
                     FASFILISRIRTSGVPDKAKQSRVWAEMKAGFRVMIGSRSLRAVMIALSVTMLGLGAV
                     NVLFVPFLIEDLDVSEAFLGAVEASQVAGLVVSGTVVALLATRLRPSTLVSAGLLGVG
                     VFVGAISGATAVWHIMVMLFFVGLSVGPVQAGANTLSQTLIEDSMRGRVGGALNTLVS
                     GANIVSMGLAGIAAAAFGTRNVFVLSGALVFLSGLLAFVLFRELRSTRSGDEAVAVAT
                     GGEQAGHE"
     gene            complement(859..1551)
                     /locus_tag="VFZ80_07440"
     CDS             complement(859..1551)
                     /locus_tag="VFZ80_07440"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HEX6287303.1"
                     /translation="MFSIFRDTNQLATVEQQITEMLASCQDTFRLATSALFGEEDVTV
                     AGDQLEETDKDLNRTERAIRRELLIHGTVRGAEVDLGLMLAYMSIAKDIERIGDYCKN
                     IWNLAQMGVSFQDADDLTELNRHRMRVAALLERALRAFSEQDGDAVHEMIPAIREDLQ
                     HYDAHIMQFVASELPGRYSAPRALWYRYLKRISAHLSNTLSSVVMPVDRLDFYKPSKA
                     VVEPDDGFETDE"
     gene            complement(1574..>2414)
                     /locus_tag="VFZ80_07445"
     CDS             complement(1574..>2414)
                     /locus_tag="VFZ80_07445"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HEX6287304.1"
                     /translation="MGANIGTTVTNTLASLGHVRQGLYFERAFAAATIHDYFNLITVA
                     ILLPLEVSFGLVSRIATWTADTVGDLLPRGGTGSSPIKDAISAPVSWLEDALTSRGWE
                     TAAGAFFLILGLGLIFIALWMITRQMRRVMSGRIESAINGILSKGAGLGALTAGLLMT
                     VAVQSSSITTSILVPLVAAGVLTLHNAFPVTLGANIGTTITAVLASLATESPDALVIA
                     LAHVTFNVLGILIIYPWPPIRAIPLWLAERTATAATRHKSLVAGYVIGIFVLAPLAIL
                     LIA"
BASE COUNT          398 a          718 c          808 g          490 t
ORIGIN      
        1 gctgctcgcc gccaactcgg tctctcagac ctcccgtatc gtcttcaatc tgctcggcac
       61 gggagcagcc ggggtccttg ccgcggtggc cccgacattg gcgccggcgt tcataatcga
      121 ctcggcgacc ttctttgcct cgttcatcct gatcagtcgc atccgcactt cgggtgtccc
      181 ggacaaggcc aagcaatccc gggtctgggc cgagatgaag gctggtttcc gagtgatgat
      241 cgggagccgg tccttgcggg ccgtgatgat tgccctatcg gtcaccatgc tcgggctggg
      301 tgcggtgaac gtgctgttcg tgcccttcct catcgaagat ctcgacgtgt ccgaggcgtt
      361 tctgggcgcg gtcgaggcca gccaggtcgc cggtctcgtg gtctccggga ccgttgtcgc
      421 cttgctcgcc acgagactga gaccctcgac gctcgtcagt gccggcctcc tcggtgtggg
      481 tgtgttcgtc ggcgccatct cgggagccac cgcggtgtgg cacatcatgg tgatgctgtt
      541 cttcgtcggg ctgtcggtgg gaccggttca ggccggggcc aacaccctga gccagacact
      601 gatcgaggat tcgatgcgtg gacgcgtagg tggcgccctc aacaccctgg tctcaggggc
      661 caacatcgtc tcgatgggtc tggccggaat cgccgcggcc gccttcggca cgcggaacgt
      721 gttcgttctc tccggggcac tggtctttct ctccggcctg ctcgccttcg tgctcttccg
      781 ggagctgagg agcacccgat ccggggacga ggccgttgcc gtcgctaccg gcggtgagca
      841 agccggtcac gaatagttct actcgtccgt ctcgaagccg tcatcgggct cgacgaccgc
      901 cttcgagggc ttgtagaagt cgagtcggtc gaccggcatg accaccgagc tcagggtgtt
      961 ggagaggtgg gcgctgattc gcttgaggta gcgataccag agtgcacgag gcgccgagta
     1021 acgaccgggc agctcggagg ccacgaactg catgatgtgc gcgtcgtagt gctggagatc
     1081 ctcacggatc gccgggatca tctcgtggac tgcgtcgccg tcctgttcgg agaaggcgcg
     1141 gagggctcgt tccagcagcg cggcgactct catccgatgc ctgttgagtt cggtcaggtc
     1201 gtcggcatcc tggaacgaga cccccatctg ggccaggttc cagatgttct tgcagtagtc
     1261 acctatgcgc tcgatgtcct tggcgatcga catgtaggcg agcatcagac cgaggtcaac
     1321 ctcggcgccg cgcaccgtgc cgtggatcaa cagctcccgt cggatcgccc gctcggtccg
     1381 attcaggtcc ttgtcggtct cctcgagctg gtcgccggcc accgtgacgt cttcctcccc
     1441 gaagagagcg gacgtggcga ggcggaatgt gtcctgacag ctggcaagca tctccgtgat
     1501 ctgctgttcc acggtggcca gctggttggt gtctctgaaa atggagaaca tggcttcctc
     1561 ctctagtgac gcactaggcg atcaacagta ttgccagggg ggcgagaacg aatattccga
     1621 tcacataacc ggcgacgagg gacttgtgtc tcgtggcagc ggtagcagtc cgttcggcca
     1681 gccacagcgg gatagcccgg attggcggcc atgggtagat gatcaggatc ccgagcacgt
     1741 tgaacgtgac gtgtgccaga gcgatcacca gggcatcggg ggattcggtg gcgagggagg
     1801 cgaggacggc agtgatagtg gtgccgatgt tcgctccgag ggtgaccggg aaggcattgt
     1861 gaagagtgag aacgccggct gccacgagcg gtacgaggat cgacgtcgtt atcgacgagc
     1921 tctggacggc aacggtcatc agtagtccgg cggtgagggc gccgagcccc gcgcctttgc
     1981 tgaggatccc gttgattgcg ctttcgatcc tccccgacat cacgcgtctc atctgacggg
     2041 tgatcatcca caaggcgatg aatatgaggc ccaggccgag tatcaggaag aaggcgccgg
     2101 ccgctgtttc ccaaccccgg gaggtcaatg cgtcctccag ccacgacacc ggtgccgaga
     2161 tggcgtcttt gatagggctc gaccccgtgc ctcctctcgg taggaggtct ccgacggtgt
     2221 cggcggtcca tgtggcgatt ctcgagacca gcccgaaaga gacctccagt gggaggagga
     2281 tggctacggt gatcaggttg aaatagtcgt ggattgtcgc tgccgcgaag gcgcgctcga
     2341 agtaaaggcc ctggcggacg tgtcccagcg aagccagcgt gttggtgacc gtcgttccga
     2401 tgtttgcgcc catg
//
LOCUS       DASUTA010000373         3898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_497682, whole genome shotgun sequence.
ACCESSION   DASUTA010000373 DASUTA010000000
VERSION     DASUTA010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497682"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VFZ80_07450"
     CDS             complement(<1..431)
                     /locus_tag="VFZ80_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX6287305.1"
                     /translation="MSLEFLAIIMFAVFLVIILSGYPVAFSFAGTAIVFFVLGVVFGD
                     LAFRELNVLFSQWFRNSVTNFTFLAIPFFVFMGAVFEKSGLAEKLLTTIGYLMGPLRG
                     GMAVAVVFVGTLLAAATGVVAATVIVMGILALPVMVRYNYS"
     gene            complement(439..993)
                     /locus_tag="VFZ80_07455"
     CDS             complement(439..993)
                     /locus_tag="VFZ80_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012596249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX6287306.1"
                     /translation="MTYQRLADAFSEWTGKIAAWVVIPTVVVGFVNVILRYTGAVVGQ
                     RLTSNAIIEAQLWLYSSIFLLGFAYVLKHQINVRVDFWYAHQPVTRRVWIDFWGNWVA
                     LIPFCIIGMWASFPQAIQSIRVGEVSADAEGLPQGPIKMLLALSFLLLLIQALSEQIK
                     LYAVLTGRGDMVHYEEAQEHLRIE"
     gene            complement(1202..2194)
                     /gene="glpX"
                     /locus_tag="VFZ80_07460"
     CDS             complement(1202..2194)
                     /gene="glpX"
                     /locus_tag="VFZ80_07460"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX6287307.1"
                     /translation="MAEPIERNLGLDLVRVTETAALGAARWLGRGDKHAVDQAAVDGM
                     RLHLSTIDVDGQVVIGEGEKDEAPMLFNGEKVGNGAGMVVDVAVDPVDGTELTAAGLP
                     NAIAVIALSEGRGSMYAPGSLVYMDKIAVGPEAADVIDLEAPVAHNLSQVAKANGKEM
                     NDLRVLILDRPRNRRYIEEARKAGARISLFRDGDVAGAIATAQESHEADILIGIGGSP
                     EAVIAAAALRCVGGEIQCRLWPRDEDEEDFAREEGLDLDQVLTSADLVDSNNIFFAAT
                     GVTNGEFLKGVNYRGEERAVTHSVIMRSKTGSVRFMTAVHNLERLRAIASVTVD"
     gene            complement(2194..3378)
                     /locus_tag="VFZ80_07465"
     CDS             complement(2194..3378)
                     /locus_tag="VFZ80_07465"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX6287308.1"
                     /translation="MTNSVITGIARTPIGRYGGVFQPLEAVDLGGVAIAAAVQRSETD
                     PSVFDEVIFGHVLQAGAGQITSRQAAVKGGLPMTVPSVTVNKVCLSGLSAIGLADRAV
                     KLGDSKFVLTGGMESMSNAPHVIRELRWGARIGNVDVIDVMQHDGLFCAFDQCTMGES
                     SDLKNSRLGIGREEQDEWAARSHQRAEEATKNGQFATEIAPVPVPQRKGDPVVIVEDE
                     GIRPGSTADSLSGLSPAFNNEGTITAGNASQISDGAAAVIVADREAAEAAGSPVVAEI
                     LGYGQIGGVDATLHERPAEALLVALKKAGLDPSDLALVEINEAFAAVSLWSARMLEIP
                     ETKVNVNGGAVALGHPLGATGARIVVTLINALRHRGGGIGAATLCGGGGQGDAIVVRV
                     EG"
     gene            3440..3850
                     /gene="mce"
                     /locus_tag="VFZ80_07470"
     CDS             3440..3850
                     /gene="mce"
                     /locus_tag="VFZ80_07470"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX6287309.1"
                     /translation="MKLLNLDHVAIAVADLDAAIDGYRDRYRVEPMYRERVESQGVEE
                     AMIPVGGSFIQLLEPLGAETPVGRFLSKNGEGLHHVAYAVVSIDDALAHLAETGAKLI
                     DTEPRIGGRGARIAFVNPGDLGGTLIELVELSDD"
BASE COUNT          713 a         1253 c         1250 g          682 t
ORIGIN      
        1 tggctgtagt tgtatctcac catcaccgga agcgccagga tgcccatcac gatcacggtg
       61 gcggcgacca caccggtggc ggcagccagg agcgttccga cgaagaccac ggcgaccgcc
      121 atcccgcccc tcagcgggcc catgagatac ccgatggtgg tcagcagctt ctctgcgagc
      181 ccggatttct cgaagaccgc ccccatgaac acgaagaacg ggatcgccag gaacgtgaag
      241 ttggtcacag agttgcggaa ccactgactg aacaggacgt tcagctcacg gaatgccagg
      301 tcgccgaaga ccacacccag cacgaagaag acgatggcgg tgccggcgaa ggagaaagcc
      361 accgggtaac cgctgaggat gatcaccaag aagacggcga acatgatgat ggcgaggaat
      421 tcgaggctca tcggtcgtct actcgatcct caagtgctct tgggcttcct catagtggac
      481 catgtccccc cggcctgtga ggacggcata gagcttgatc tgctccgaaa gcgcctggat
      541 gagcaggagc agaaaagaca acgcaagcag catctttatc ggtccctggg gcagaccctc
      601 ggcgtctgcg gacacctcac cgactcgaat cgattggatc gcctgtggga aggaggccca
      661 catcccgatg atgcaaaacg ggatcaaagc gacccagttg ccccagaagt cgatccagac
      721 ccgccgcgtc acgggctgat gggcatacca gaaatcgacc ctgacgttga tctgatgttt
      781 gagcacgtag gcaaacccga gcaggaagat cgacgaatag agccagagct gggcctcgat
      841 gattgcgttg ctggtcagtc tctgtccgac caccgcgccg gtgtagcgga ggatcacgtt
      901 gacgaacccc acgacgacag tggggatcac gacccaggcg gcgatcttgc ccgtccactc
      961 cgagaaggcg tccgccaagc gctgataggt caacagcgcc ccgaagaccg gccctacctc
     1021 tctgctcccg gtttcgcttg tcgtcgcatc ctcctggcgg gggtgggggg caccctacat
     1081 gggaacgacg agagtggttg gagaactgga ccgatgtctc tcgcgtcgag cccgcgaggg
     1141 gttgctcgta tcccggacgc tgtgccggtt cgaagtgtgc aggtgaagtc cgtggctggc
     1201 gtcagtcgac tgtgacgctg gcgatggcgc gaagtcgctc caggttgtgg accgcggtca
     1261 tgaagcgaac cgacccggtc ttggaccgca tgatcaccga gtgggtgacg gctcgctcct
     1321 ccccccggta gttgacgccc ttcaagaact cgccgttggt gaccccggtc gccgcgaaga
     1381 atatgttgtt cgagtcgacg agatcagcgc tggtcagcac ctggtcgagg tcgagaccct
     1441 cctcccgggc gaagtcctcc tcgtcctcgt cacggggcca gagccggcac tggatctcac
     1501 cgccgacgca acgcagagcg gcggcggcga tcacggcctc gggtgacccg ccgatgccaa
     1561 tcaggatgtc ggcctcgtga ctctcctgtg ccgtggcaat ggcgccggcc acgtctccat
     1621 cccggaacag cgatatgcgg gctcctgcct tgcgcgcctc ttcgatgtag cgtcgattgc
     1681 gaggacgatc gaggatgagg acccgcaggt cgttcatctc cttcccgttg gctttggcga
     1741 cctggctcaa gttgtgcgcc acgggcgcct cgagatcgat cacgtccgcg gcctccgggc
     1801 cgacggcgat cttgtccatg tacacgagtg agcccggggc atacatcgac ccccggccct
     1861 ccgacaatgc gatgacggcg atggcattgg gcaggccggc cgcggtcagc tcggtgccgt
     1921 ccaccgggtc gacggcgacg tcgacgacca tgccggcacc gttgcccacc ttctccccat
     1981 tgaagagcat cggcgcctcg tccttctctc cctcgccaat gaccacctgt ccatcgacgt
     2041 cgatcgtgga gagatggagg cgcataccgt ccacggctgc ctggtcgacc gcgtgtttgt
     2101 cgcctctgcc cagccatcga gcggcgccga gggcggcagt ctcggtcacc ctgaccaggt
     2161 cgaggcccag attgcgctcg atgggctcgg ccatcagcct tcgactctca cgacgatggc
     2221 gtccccctgt ccgccaccac cgcacagagt ggcggcccct atccccccac cacggtgacg
     2281 cagggcattg atcagggtga cgacgatgcg ggcgccggtg gctccgaggg ggtgaccgag
     2341 cgcaacggcg ccaccgttca cgttcacctt ggtctccggg atctcgagca tgcgcgccga
     2401 ccagagtgac accgcggcga acgcctcgtt gatctcgact agagcgaggt cggacgggtc
     2461 gagaccagct ttcttgagtg ccaccagcag agcctcggcc ggccgctcgt ggagtgtggc
     2521 gtcgacacct ccgatctgcc cgtagcccag gatctcggcg actacgggcg aaccggccgc
     2581 ctccgccgcc tcccggtcgg cgacgatcac tgcggcggca ccgtccgaga tctgtgaggc
     2641 gttgccggcg gtgatggtgc cctcgttgtt gaaagcaggc gagagccccg acaaggagtc
     2701 cgcggtggaa ccgggtcgaa tgccctcgtc ctcgacgatg acgactggat cgccctttcg
     2761 ctgcgggacc ggtaccggag cgatctcggt ggcgaactga ccgttcttgg tcgcctcctc
     2821 ggcgcgctga tggctgcgtg cggcccattc gtcctgctcc tcgcgcccga tcccgagccg
     2881 ggagttcttg agatcggacg actcgcccat ggtgcattgg tcgaaagcgc aaaacaggcc
     2941 gtcgtgctgc atcacgtcga tgacatccac gtttccgatt cgagcacccc aacgcagctc
     3001 gcggatcaca tgaggggcgt tggacatcga ctccatgccg ccggtgagga cgaacttgga
     3061 gtcaccgagc tttaccgcgc gatcggcgag accgatcgcc gacaaccccg ataggcagac
     3121 cttgttgacc gtcaccgagg gaacagtcat gggcaggccg cccttcaccg ctgcttgtct
     3181 cgacgtgatc tgaccggcgc ccgcctgtag gacatggccg aagatgacct cgtcgaagac
     3241 ggagggatcg gtctccgatc gctgcactgc ggcggcgatg gcgacaccgc cgagatccac
     3301 ggcctcgagc ggttggaaga cccctccgta tcgtccgatg ggtgtccggg cgatgccggt
     3361 gatgactgag ttggtcacga tgtcgtgatg gtaccgggcg caagtgacag tcccgccccg
     3421 tcgcaatacg ctccgtgacg tgaagctcct caacctcgat catgttgcga tcgccgtcgc
     3481 cgatctggac gcggcgatcg acgggtaccg cgaccgctac cgcgtcgagc cgatgtatcg
     3541 ggagagagtg gagagtcaag gtgtcgagga ggcgatgatc ccggtcgggg ggtcgttcat
     3601 ccagctgctc gagcccctcg gggctgaaac cccggtggga cgtttcttgt cgaagaatgg
     3661 tgaaggtctc catcacgtcg cctacgcggt cgtctcgatc gacgatgccc tcgcccattt
     3721 ggccgagacg ggggcgaagc tcatcgacac cgagcctcgt atcggaggac gtggcgcccg
     3781 gatcgccttc gtcaaccccg gagacctcgg gggtaccctc atcgagttgg tggaactgag
     3841 cgatgactga tgagatcgag atcaaaggcg gcgccggcga gttcgaggct gccgtgat
//
LOCUS       DASUTA010000374         2046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1925159, whole genome shotgun sequence.
ACCESSION   DASUTA010000374 DASUTA010000000
VERSION     DASUTA010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1925159"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /gene="aceK"
                     /locus_tag="VFZ80_07475"
     CDS             <1..1213
                     /gene="aceK"
                     /locus_tag="VFZ80_07475"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HEX6287310.1"
                     /translation="ASRIEERLAERGGLPVVGRAEIVRSVFYRGQSAYIVGLLYAGSV
                     RLPMVLSLRHEVGGVVVDAVLLTEDEASILFSFTRSYFHVEVTRPYDLVRFLRRLMPR
                     KRIAELYISIGQNKHGKTELYRDLLRHLNSTDERFEYVPGTQGLVMIVFGMPGYDDVF
                     KVIRDSFPPPKRTTRNTIMEKYRLVSQHDRAGRLIDAQNFQHLSFHRSRFDPALLDEL
                     VDQAARTVRLDGEEVVISHLYVERRVIPLDLYAREAVPSAATSAVIDYGQAIKDLACT
                     NIFPGDLLLKNFGVTRHGRVVFYDYDELSALTDVIFREMPEPRDETEALSEAPWFSVG
                     DNDVFPEEHQRFLGLKRELREALHERHGDLFEVEPWKAIQKRITAGELMEIFPYGDEA
                     RLPATEDVRGW"
     gene            1295..2020
                     /locus_tag="VFZ80_07480"
     CDS             1295..2020
                     /locus_tag="VFZ80_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6287311.1"
                     /translation="MTDLVVVTGASSGLGLALARSVPFPAQVVDISRSGPPEGGGIDH
                     FPADLSQPSDWASVGDELKRLINQLEPERAVMIHAAGTLTPIGFAGEVDALAYTSNVL
                     LNSAAGQVLGHHFLSAVAGLEGDYDLVMISSGAASSVYAGWSPYGAGKAALDQWVRNV
                     GAEQKLRGGVRVSAIAPGVLDTAMQEEIRSTTQDDFPNVDRFHDLHDQGDLVDPDVVA
                     GRVWEVIENGIETGSVLDVRRLQ"
BASE COUNT          378 a          660 c          630 g          378 t
ORIGIN      
        1 cgcgtcccga atcgaagaac gtctcgccga acgcggtggc ctgcccgtcg ttggtcgggc
       61 cgagatagtg cgctccgtct tctaccgggg ccaatccgcc tacatagtcg gactcctgta
      121 tgccggctcg gttcggttgc ccatggtgct gtcccttcgt cacgaggtgg gcggcgtcgt
      181 ggtggatgca gtgctactca ccgaggacga ggcatcgatt ctcttcagct tcacacgctc
      241 ctacttccac gtcgaagtca cccgtcccta tgacctggtc cggttcttgc gcagactcat
      301 gccccgcaag cggatcgccg agctctacat ctcgatcggc cagaacaagc atggcaagac
      361 agagctctac cgcgacctcc tacgtcacct gaactccact gacgagcgct tcgagtacgt
      421 gcccggcacc cagggcctgg tgatgatcgt gttcgggatg cccggctatg acgatgtctt
      481 caaggtgatc agagacagct tcccgccacc gaagcgcact acccgtaaca cgatcatgga
      541 gaagtatcgg ctcgtctccc aacacgaccg ggccggccgt ctcatcgacg cgcagaactt
      601 ccaacatctc tcgttccacc gatcgaggtt cgacccggct ctgctcgacg agctggtcga
      661 ccaggcggcg cgcaccgtcc ggctggatgg agaggaggtc gtcatcagcc acctctacgt
      721 ggagagacgg gtcatcccac tcgatctgta cgcccgggag gccgtgccct cggcagccac
      781 ctcggcggtg atcgattacg gccaggcgat caaggacctc gcctgcacca acatcttccc
      841 cggggacctc ctgctgaaga acttcggagt cacccggcat ggccgtgtcg tgttctatga
      901 ctacgacgag ctcagcgctc tgaccgatgt catctttcgt gagatgcccg agccgagaga
      961 cgagaccgag gcgttgagcg aggcgccatg gttctcggtc ggtgacaacg acgtgtttcc
     1021 cgaagagcac cagcggttcc tcgggctcaa acgagagctg cgcgaagcgc ttcacgagcg
     1081 acatggggat ctcttcgagg tcgagccgtg gaaggcgatc cagaagcgca tcaccgcggg
     1141 cgagctcatg gagatcttcc cctatgggga cgaggcgagg ttgcccgcca ccgaggacgt
     1201 gcgaggctgg tgaccgaggc gttgctatag caagatgact cggaatccac gaccctgtgc
     1261 acatccatca cgaggtgggt catagcgtgc ggacatgacc gacctcgtcg tcgtcaccgg
     1321 tgcatccagc ggcctcggcc tggccctggc ccgatcggtc ccgttcccgg ctcaggtcgt
     1381 cgacatcagc agatcgggcc caccggaagg cggcgggatc gaccacttcc ccgctgacct
     1441 ctctcagcct tccgattggg ccagtgtcgg agacgagctg aagcgtctga tcaaccagct
     1501 cgagcccgag agggcagtaa tgatccacgc cgccgggaca ctgacaccga tcgggttcgc
     1561 cggcgaggtc gacgcactcg cctacacgtc caacgtcctc ctcaacagtg ctgcgggaca
     1621 ggtcttgggc catcacttcc tctcggcggt agccggtctc gaaggcgact acgacctcgt
     1681 gatgatcagc tccggagcag cctctagcgt ctatgccggg tggtccccgt acggagcagg
     1741 caaggctgct ctcgaccagt gggtgcgaaa tgtgggagcc gagcagaagc tccggggcgg
     1801 tgttcgggtg tcggcgatcg cccccggggt gttggacacg gcgatgcaag aggagatccg
     1861 gtcgacgact caagacgatt tccccaacgt cgaccggttt cacgacctcc atgaccaggg
     1921 cgatctggtc gaccccgatg tcgtcgccgg tcgagtatgg gaagtgatcg agaatggaat
     1981 cgagaccggc tcggtcctcg atgtcagacg gctccaatga ctctgggctc gatccgcagt
     2041 cgctct
//
LOCUS       DASUTA010000375         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1276609, whole genome shotgun sequence.
ACCESSION   DASUTA010000375 DASUTA010000000
VERSION     DASUTA010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276609"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VFZ80_07485"
     CDS             <1..665
                     /locus_tag="VFZ80_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287312.1"
                     /translation="ESEGVLPISGGEDYQTIVGDSLGMTTAGSQTIVCDPLELIGVVL
                     EDPALLGEYPGPYGVAISAPFDLQPYLFDADDADDGTYAAFASEILADRGLEVDAPVI
                     KQLVRTDLEGDGVNEVLVVAEEVTPGLLLQPGDYSILFMRKVIEGEVQTAILEVTVVL
                     DEDDQFLGAHSVGTVADLNGDGKMEIVSNSAFFEGFGVVIWEYVNDDLGLNLRLERGC
                     GS"
     gene            665..1072
                     /locus_tag="VFZ80_07490"
     CDS             665..1072
                     /locus_tag="VFZ80_07490"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013474933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX6287313.1"
                     /translation="MENPFTNMTTFVSHSGLDLTDASADSAAGRIEIADYHQQPYGVV
                     HGGVYCMMIETLASTGAALWAMENGMAGAVGLSNKTDFIRATTDGVIVGVATPIHRGR
                     TQQLWQVDITRESDGKLVAQGQVRLQNVSEIAF"
     gene            1106..1906
                     /locus_tag="VFZ80_07495"
     CDS             1106..1906
                     /locus_tag="VFZ80_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX6287314.1"
                     /translation="MPEGDSVAGHASRLRPILLGRKITDVKGTAPAVRANSHRILDAT
                     VEDIRTVGKNLVIDLSGGFSIRVHLGMSGRWRMIPPDRTIPGSARLALSAGGSAAVCS
                     AAPTIDVDRTPVIDYSLSRIGPDVLGDFELDEFVRRARTRRSVAVADVLLDQRVLAGI
                     GNVYKSELLFLARIDPHTPIDEISDDRLRRVAKDAIRLMTANVGPKPRTTTGSRARDR
                     GMWVYGRARQPCRRCGTAVSMENQGGRVTYWCSKCQPSSPPGPVSGSM"
BASE COUNT          368 a          596 c          656 g          380 t
ORIGIN      
        1 cggagtcgga gggcgttctc ccgatctccg gcggggagga ctaccagacc atcgtcggag
       61 acagtcttgg gatgaccacc gccgggtcgc agaccatcgt atgcgatccg ctcgagctga
      121 tcggagtggt gctcgaggat ccggccctgc tcggtgagta cccggggccc tatggcgtgg
      181 cgatctcggc acccttcgat ctccagccct atctcttcga tgcagatgac gccgatgatg
      241 ggacgtatgc cgcattcgca tccgaaattc tcgccgaccg aggcctcgaa gtggacgctc
      301 ccgtgatcaa acagctggtt cgcacagatc tggagggtga tggagtcaac gaggtgctcg
      361 tcgtggccga agaggtgacg ccgggtctgc ttctgcagcc gggcgattac tccatcctct
      421 tcatgagaaa ggtgatcgaa ggcgaggtgc agaccgcgat cctggaagtg acggtggtgt
      481 tggacgagga cgatcagttc ctcggggcac attcagtggg caccgtggcc gacctcaacg
      541 gcgatgggaa gatggagatc gtgtccaact cggccttctt cgaggggttc ggtgtggtga
      601 tctgggagta tgtgaacgat gatctcgggc tgaacctgcg tttggagaga ggctgcggta
      661 gctgatggag aacccattca cgaacatgac gacctttgtc agccactccg gtctcgacct
      721 caccgacgcc tcagctgact cagccgcagg tcgtatcgag atcgccgact atcaccaaca
      781 gccgtatggg gtggtccacg gtggggtcta ctgcatgatg atcgagacgc ttgcatccac
      841 cggcgccgcc ctgtgggcaa tggagaatgg gatggcgggc gccgtcggtc tgtccaacaa
      901 gacggacttc ataagggcga cgaccgatgg ggtgatagtc ggcgtggcaa ctccgattca
      961 tcggggtcgc actcagcagt tgtggcaggt cgacatcacg agagagtcgg atggcaagct
     1021 cgtggcccag ggccaggtgc gcctccagaa cgtcagcgag atcgccttct gatggacacc
     1081 ttcgaagtag tcaggaggca accgcttgcc tgagggcgac tcggtagccg gacatgcctc
     1141 caggctgcgc ccgatattgc tcggtcgcaa gatcaccgac gtcaagggga cggctcccgc
     1201 agtcagggcg aactcccacc gcatcctcga cgccacggtc gaggacatca ggacggtagg
     1261 aaagaacctg gtcatcgacc tctccggtgg cttctcgatc agggtgcatc tcgggatgtc
     1321 cggccgatgg cgaatgatcc ccccggaccg gacgattcca ggctcggctc gccttgcgct
     1381 ctcggccggc ggctcagcag cggtatgcag cgccgcacca acgatcgacg tcgatcgcac
     1441 accggtcatc gactacagcc tgagccggat cggccccgac gtcctcgggg atttcgagct
     1501 cgatgagttc gttcgtcgag cccgcacccg acgttccgtc gccgtcgctg atgtgctcct
     1561 cgatcagagg gtgctggcag gcatcggcaa tgtgtacaag tcggagcttc tgttccttgc
     1621 gcggatcgac ccgcatactc cgatagacga gatctccgac gatcgtctgc gtcgggtcgc
     1681 gaaggacgcg atccggctca tgacagccaa tgtcggtccg aagccacgca cgacaaccgg
     1741 ctcccgtgcc cgggatcggg ggatgtgggt ctacgggcga gcacggcagc cctgtcgtcg
     1801 ttgtggaacc gctgtttcga tggaaaacca ggggggaagg gtcacctact ggtgctcgaa
     1861 gtgtcagcct tcgtcgccac cggggcctgt gtctggctcg atgtgaaggc cctcggctgc
     1921 cctcgccagg cgaggcaagt agtgcttcca cccccatcat gtggacttcg acctcgtcgt
     1981 cgggcaagcg atgggcgctg
//
LOCUS       DASUTA010000376         4180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_886355, whole genome shotgun sequence.
ACCESSION   DASUTA010000376 DASUTA010000000
VERSION     DASUTA010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886355"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..907)
                     /locus_tag="VFZ80_07500"
     CDS             complement(212..907)
                     /locus_tag="VFZ80_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6287315.1"
                     /translation="MLDVDQIDVSIDSKPILSDVSLQVADSKIVAILGPSGSGKSTLL
                     RAIAGLVDVAGGEIRWDGRSITQTPPHRRDFGLMFQGYALFPHMTVAGNVGFGLRMAG
                     RDDIDRRVSEALSWVGLDGFEPRQVDSLSGGEQQRVALARTLASEPRLVMLDEPLGAL
                     DRNLRQQLVADTRQVLEEREVTAIVVTHDREEAVALSDRLALMREGTIVQTGRLDEIL
                     DMPADDWVRRFIS"
     gene            complement(907..2457)
                     /locus_tag="VFZ80_07505"
     CDS             complement(907..2457)
                     /locus_tag="VFZ80_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6287316.1"
                     /translation="MRPVRWGWVAVAAIPTIFIGYFFVYPLVRILGLGLSELSIGASG
                     VEARLFRVGWFTVWQAVVSTILTFLAAAPLTWAVSTYRFKGRRVAMALATVPFVMPTV
                     VVGTAFVALGFEESIVAILAAHVFFNVAVVVRTVSTLWSRIDPRILDAGRVLGASNWQ
                     VFRTITLPLLRPALAAAASIVFLFTFTSFGVVLILGGFQYATLEVEIYRQAVTLFDLP
                     LAATLAIVQLVGVSGALYWYSRYQERTAVAWTLERGTNLRRPRGRSRWAVATAVIGSL
                     CVLMIPLGVLVGRSLSGDFYRELFSDDRVAGTPISSVGNSLVFATLAMVLATFIGVLA
                     ATVISERSGSLSRWFDLTLMLPLGTSAVTIGFGFIVALDWPVDLRGSYWLIPIAHALV
                     AVPFVVRSTVPTLRSVPNETREAAAVLGASPFRVWREIDLPIVTRAALIGAGFAFVVS
                     LGEFGATSFIARPNTATIPVMIFRLLGRPGSSSFGTAMALSVVLALLTAGVVLAIDRA
                     RFGELGSF"
     gene            complement(2457..3512)
                     /locus_tag="VFZ80_07510"
     CDS             complement(2457..3512)
                     /locus_tag="VFZ80_07510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6287317.1"
                     /translation="MRHTRILTALLIALATVLTACGDDEPEQTGTPETLTILAHDSFA
                     DAVSDETFSAFESDTGIVVEVLAGGDAGSVVNQAVLSADNPIADVLFGVDDTFLSRAL
                     EEEIFVPHESEHIGTVPDSFIEEENRVTPIDYGDVCFNYDKEYFAESDIGIPDDLDDL
                     TADRYAGLVTVEHPATSSPGLAFMLATIDRYGEDGWLDWWSEMKAAGINVVSDWDTAY
                     YAAFTRYGGDSPIVMSYASSPPAEVIFAEEPLEEAPTGVIEAGCYRQIEYAGVLSGTE
                     WPQSAGDLIDFMLSVEFQETIPENWFVFPANQEADLPQSFVEHTVIPEDPADLDADVI
                     ADNRDEWIDDWISVMEG"
     regulatory      complement(3535..3645)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3700..>4180)
                     /locus_tag="VFZ80_07515"
     CDS             complement(3700..>4180)
                     /locus_tag="VFZ80_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="FabF, beta-Ketoacyl-ACP synthase II, KASII;
                     catalyzes a condensation reaction in fatty acid
                     biosynthesis: addition of an acyl acceptor of two carbons
                     from malonyl-ACP; required for the elongation of
                     short-chain unsaturated acyl-ACP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEX6287318.1"
                     /translation="ARVLGLVLGCGERADSFHRTRSNPDGSAIIGAMRGAIEDAGLEP
                     SDVAYINAHGTGTDLNDKTEGAVIHRIFGDDQPAVSSIKGSTGHGLGASGSIEAAIVV
                     EAIRRRELPMNTGLTNQDPDIPLNNIVREVRSWEPGPVLSNSFGFGGHNTVVAIGPV"
BASE COUNT          807 a         1304 c         1395 g          674 t
ORIGIN      
        1 cggacgagct ccgagagatc ttctccgatc tcaaagactc tcgatccgac gagaacgcct
       61 agggcttcaa actcctccat tcgacaaccg caagcccagg tacagacggg gctccgggtc
      121 actcctcccg gggccccgtc tctcgtcgtg ctcgcgcgca cgacgttgcg ctcagcgcaa
      181 cgcggtgccc gcgggcagag cgaattgccg gtcagctgat gaatcgccgg acccaatcat
      241 cggccggcat atcgaggatc tcgtcgagtc gcccggtttg gacgatggtg ccctccctca
      301 tgagggcgag tcgatcggag agggcgaccg cctcttcccg gtcgtgggtg acgacgattg
      361 cagtcacctc acgctcctcg agcacctgcc gggtgtcggc gacgagttgc tgtcgcagat
      421 ttcggtcaag tgccccgagt ggctcgtcga gcatcacaag tcgtggctcg gaggcgagag
      481 tcctcgccag ggcgacgcgc tgttgttcgc caccggagag cgagtcgacc tgacggggct
      541 cgaagccatc caggcccacc cacgacaggg cctcactcac cctccgatcg atgtcgtcgc
      601 ggccggccat gcggaggcca aagccgacgt tgccggcgac agtcatgtgt gggaacagcg
      661 cgtatccctg gaacatgagc ccgaagtcgc gacgatgggg tggcgtctgc gtgatcgagc
      721 gaccgtccca ccggatctcc ccgccagcca catccaccag accggctatg gcgcggagaa
      781 gggtcgactt ccccgagccc gaggggccga ggatggccac gatcttgcta tcggcaacct
      841 ggagggacac gtctgacaga atcggcttgg agtcgatgct gacgtcgatt tggtcgacat
      901 cgagcatcag aaggatccca actcgccaaa gcgggcgcga tcgatcgcca ggacgacgcc
      961 agcggtgagc aaggcgagga cgaccgacag ggccatggcc gtgccgaacg acgagctacc
     1021 aggcctgccg aggagacgga agatcatcac cgggatggtt gcagtgttgg gcctggcgat
     1081 gaaagacgtg gctccgaatt caccgagcga taccacgaag gcgaacccgg ccccgatcag
     1141 tgcggctcgg gtgacgatgg ggaggtcgat ctcacgccac acccgaaatg gcgaggcgcc
     1201 aagcaccgct gctgcctctc gcgtctcgtt cgggaccgag cgcagtgtgg ggactgtcga
     1261 cctcaccacg aaaggcacgg cgaccagggc gtgcgcgatc gggatcagcc agtaagaccc
     1321 cctgagatcg acaggccagt cgagggcgac gatgaaaccg aaaccgatgg tcaccgccga
     1381 ggtccccagc gggagcatca gggtcaggtc gaaccaacga gacaaggacc ccgacctctc
     1441 actgatcacg gttgcagcga ggacgccgat gaaagtggca aggaccatcg ccagggtggc
     1501 aaacaccagg ctgttcccga cggatgagat cggcgttccc gcgacgcggt cgtcgctgaa
     1561 gagctcccgg tagaagtcgc cggagagtga tcggccgacc agaacaccca aggggatcat
     1621 cagcacgcag agtgagccga tcaccgccgt cgccacggcc cagcgggacc gtcctcgagg
     1681 acggcgcagg ttcgttcccc gctcgagggt ccaagccacc gcggtccgct cttgatatcg
     1741 cgagtaccag tagagggctc cgctgacgcc gaccaactgg acgatggcca gggtcgcggc
     1801 gagagggagg tcgaagagag tcactgcctg gcggtagatc tccacttcga gggtggcgta
     1861 ctggaacccc ccgaggatca gcacgacgcc gaaggatgtg aacgtgaaca ggaagacgat
     1921 tgaggcggcc gcggccaggg cggggcggag gagagggagc gtgatggtcc ggaacacctg
     1981 ccagttggag gcaccgagca ctcggccggc atcgaggatg cgggggtcga tgcgtgacca
     2041 cagggtggaa accgtcctca ccacgacggc gacattgaag aacacgtggg cggcgaggat
     2101 ggccacgatc gactcttcga agcccaacgc cacgaaagcg gtgccgacga ccactgtggg
     2161 catgacgaat gggaccgtgg ccagggccat cgccacccgt cgtcccttga accgataggt
     2221 agagacagcc caggtcagtg gggccgcagc caggaaggtg aggattgtcg agaccacggc
     2281 ctgccagacc gtgaaccagc cgacacggaa cagccgcgcc tccactccgc tggctccgat
     2341 gctgagctct gacaggccga ggccgaggat gcggaccagc gggtatacga agaagtagcc
     2401 gatgaagatg gttgggatgg cggccacggc cacccatccc catctgactg gtcgcatcag
     2461 ccttccatca cggagatcca gtcatcgatc cactcgtctc ggttgtcggc gatgacatcg
     2521 gcgtcgagat cggcgggatc ttcggggatg acggtgtgct cgacgaagga ctggggcaga
     2581 tcggcctcct ggttcgccgg gaagacgaac cagttctcgg ggatggtctc ctggaattcg
     2641 accgagagca tgaagtcgat caggtccccc gccgattgtg gccactcggt gccggaaagg
     2701 accccggcat actcgatctg gcgatagcag ccggcctcga tgactccggt cggagcctcc
     2761 tcgagtggct cttcggcgaa gatgacctcg gcaggcgggg aggaggcgta ggacatgacg
     2821 atcgggctgt ctccgccgta gcgggtgaag gctgcgtagt aggccgtgtc ccaatcactc
     2881 acgacgttga tgcccgcggc tttcatctcg ctccaccaat cgagccagcc gtcctcgcca
     2941 tatcggtcga tggtggccag catgaaggcc agacccggcg acgaggtggc agggtgctcg
     3001 acggtcacca ggccggcata ccggtcggcc gtgaggtcgt cgaggtcatc ggggataccg
     3061 atgtccgatt cggcgaaata ctccttgtcg tagttgaagc aaacgtcgcc gtagtcgatc
     3121 ggggtcacac gattctcctc ttcgatgaag gagtcgggaa ccgtgccgat gtgctccgac
     3181 tcatgaggaa cgaagatttc ctcttcgagg gcgcgggaca ggaaggtgtc gtccacaccg
     3241 aacaatacgt cagcgatcgg gttgtcggcc gacagcaccg cctgattgac caccgagccg
     3301 gcgtcaccgc cggcgaggac ctcgacgacg atcccggtgt ccgactcgaa ggcggagaag
     3361 gtctcgtcac tcacggcgtc ggcgaacgag tcgtgggcga gaatcgtcag cgtctccggc
     3421 gtgccggtct gttccggctc gtcgtcgcca caagcggtta ggaccgtggc cagcgcgatc
     3481 agtagggctg tcaggatgcg tgtgtgtctc atcgtctcct tttcgttccg ggagcgatga
     3541 gcaatccgag tccctgcgcc ggaattaccc gtctcaggtt caagcggtcg gagtctctca
     3601 gactccctct cagcccggtg tcaccgagct cccgttgtca ccagtgtagg agctgacccg
     3661 gctatcccga ccagtccact cgtccgacac gtcagacgtc taaacgggcc cgattgccac
     3721 cacggtgttg tgaccgccga aaccgaagga gttggagaga accggtcccg gttcccagga
     3781 gcgcacctcg cgcacgatgt tattgagggg gatgtcgggg tcctggttgg tgagaccggt
     3841 gttcatcggc agctctctgc gccggatcgc ctccacgacg atggccgcct cgatcgaccc
     3901 cgaggcgccg agaccatgcc ccgtcgaccc tttgatcgag gaaaccgccg gctgatcgtc
     3961 tccgaagatg cgatggatga cggcgccctc ggtcttgtcg ttgaggtcgg tccccgttcc
     4021 gtgagcgttg atgtaggcga cgtcggacgg ctcgagcccg gcatcctcga tggcgccgcg
     4081 catggcgccg atgatggcgc tgccgtccgg gttgctgcgg gtgcggtgga agctgtccgc
     4141 ccgctcgccg cagcctagga ccaggccgag cacccgcgcg
//
LOCUS       DASUTA010000377         6369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1148049, whole genome shotgun sequence.
ACCESSION   DASUTA010000377 DASUTA010000000
VERSION     DASUTA010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148049"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFZ80_07520"
     CDS             <1..240
                     /locus_tag="VFZ80_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6287319.1"
                     /translation="FLFSGTFFPITQLPDWLEPVAYITPLFHGVELTRMVALPGEGSP
                     VITGMPAWIHLAYLLAMAGLGIYFSIKSLDRRLRL"
     gene            237..1046
                     /locus_tag="VFZ80_07525"
     CDS             237..1046
                     /locus_tag="VFZ80_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6287320.1"
                     /translation="MSVSAPSRITPVGRITPFRAQRIWEKNYLAYRRLWKVVFSGFFE
                     PVFYLFAVGVGVGALVGDVPLPDGTLVPYAAWVAPALMAASAMNGAVIETTFNIFFKL
                     QFGKVYEGILTTPMQPMDIAIGEIGWALLRGLLYSTGFIVIMFLMGMTESWTAIFAVP
                     SATLVGFAFGAVGLAAATWMKSWQDFDLVTLVTMPLFLFSGTFYPLDVYPPVLQTVAK
                     FSPLYHGTELLRGFTLGPIDWTMIGHAAFLVVMGLVGASIAARRLDGMLRK"
     gene            1111..2778
                     /gene="hutU"
                     /locus_tag="VFZ80_07530"
     CDS             1111..2778
                     /gene="hutU"
                     /locus_tag="VFZ80_07530"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX6287321.1"
                     /translation="MLDLAFPLHAPRGSELNTKGWLQEGALRCLLNNLDPDVAEDPEN
                     LVVYGGRGKAARNIDALHAIVRTLERLENDETMLVQSGKPVGVFRTHDLAPRVLIANS
                     LLVPRWATLDHFWDLEAKGLMMYGQMTAGSWIYIGTQGILQGTYQTFVAIAEKRFGGT
                     LAGTLTLTGGLGGMGGAQPLAITMNGGVALCVEVDPHRIERRLETGYLDVQADSVEHA
                     IELATEAKAAKKALSIGLLGNAAELFPQLLESDLDVDIVTDQTSAHDPLYGYIPSGYT
                     LEEAASLRQHDPDTYIKAARESMATQCEAMVGFQRAGAEVFDYGNNLRGEAQTAGFSD
                     AFAYPGFVPAYIRDLFAEGSGPFRWVALSGEPADIETTDRALVELFPDNEPLLRWLRL
                     AGERVHYQGLPARICWLSYGQRHLAGLRFNELVANGEVSAPIVIGRDHLDSGSVASPF
                     RETEAMADGSDAVADWPILNAMLNVSSGAAWVAVHHGGGVGIGNSIHAGAQVVADGTD
                     QGAFRVERVLTNDPGTGVMRHADAGYEKAQRVARERGVDIPMLDEGD"
     gene            2771..3670
                     /locus_tag="VFZ80_07535"
     CDS             2771..3670
                     /locus_tag="VFZ80_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HEX6287322.1"
                     /translation="MIDPHWPRAGVWLASDAPEPDLVVVGVPNSRSSLTHSDAALAPL
                     RMRERLDRFSTFHGETETDLSAVKVSDFGNWPVSEIDPIALIGEIQQWAAELPDVALR
                     IYLGGDNAITRPLVAAQSDEPERIGLITFDAHHDVRTLETGPSNGNPIRGLIEEHGLP
                     GRNIVQIGIHSFANSNAYREYCEQEGVTTVTVAQIENIGMEEAVDVALSRLSATCDRI
                     YVDVDIDVLDRAYAPACSGARPGGLSVRQLAHGVTRCASHPIVRAIDFVEVDPSADRD
                     DQTIDVMAHLFLSAVAGLATQPG"
     gene            complement(3667..4782)
                     /gene="dusB"
                     /locus_tag="VFZ80_07540"
     CDS             complement(3667..4782)
                     /gene="dusB"
                     /locus_tag="VFZ80_07540"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX6287323.1"
                     /translation="MTLSIGSLRVDPPVVLAPMAGVTNAPFRRLCREFGAGLYVSEMI
                     GARGLVERNEKTLALARFDEDEVPRSIQLYGTDPGALGEAVSFLVEEQGVHHIDMNFG
                     CPMGKVTRHGGGAALPWKTDLFRSIVRAAVSNAGDVPVTVKFRKGIDDEHLTYIDTGL
                     VAEDEGAAAVALHARTARQLYSGEADWTAIARLREAVQSIPVLGNGDIWTADDAVRMM
                     ESTGCAGVVVGRGCLGRPWLFRDLADGFAGRQLQEPPSLGEVGQIMIRHAQLLVDHFG
                     ERFGIRLFRKHPSWYLKGFPAGPAIRDAMSRVSSVDELADLVGKLDSDVAFPEAANRM
                     VRGHSHGPRPVRLPHDFLIDRSTHHLAPEADQLVSGG"
     gene            4803..6227
                     /locus_tag="VFZ80_07545"
     CDS             4803..6227
                     /locus_tag="VFZ80_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287324.1"
                     /translation="MELPIPTGTSKPQMRRALIAGLPLALLLAACGGIGPGTEPARTS
                     QSPGTSAPPVTTPATPPPTTGALLRCDQVPLLTADPSYYRDTPTYIGNEMPVDEVRRW
                     AAQYADFVDIWIDRDHNGWVTVAFTEDVADRQAEIEREFPEDGVVALQIELTEEELSS
                     LQKEVVEGLDGVVDVLGAWTDVIRGHVNVQIPVLSEENLSAIEALFPDAPICVEGLPP
                     EMFVEPGPQPESGDGWQLIYEQKHAGFPYVTGLAWDQESFHALLDDIEGIAELDVDVD
                     FENQVVIWFGAVFGSSCPNIRMDDVVIDGDTIYPLIVDTDNSFACTSDANPHTYLVGL
                     DRDRLPAPPFYVQIYPDLDFDRVLVEADLRPAGSTALPGEVGPDPNPPGPKPERSGAI
                     IEPGYPWQYEIDLTCGWEWLGEINSYQWVATGPIPESWLAKTDGESAVTVEVTLNEGG
                     PDPFVEVVFDGQSVIYEIGRSPGC"
BASE COUNT         1135 a         2063 c         2020 g         1151 t
ORIGIN      
        1 ttcctgttct cgggcacgtt cttcccgatc acccagctac ccgactggct cgagcccgtg
       61 gcctacatca caccgctgtt ccatggtgtc gaactgacgc ggatggtggc tcttcctggc
      121 gaaggctccc cggtgatcac cggcatgccg gcatggatcc acctcgccta tctgcttgct
      181 atggccggtc tgggcatcta cttctcgatc aagtcgctcg acaggagact ccggctgtga
      241 gcgtctcggc tccgagccga atcacccccg tcggcaggat caccccattc cgcgcccaga
      301 gaatctggga gaagaactac ctcgcatacc gacggctgtg gaaggtcgtg ttcagcgggt
      361 tcttcgagcc agtcttctat ctcttcgccg tcggagtcgg ggtcggggca ctcgtgggtg
      421 acgtcccgct ccccgacggg acactcgtgc cgtatgccgc atgggtggcg ccggcgctga
      481 tggcggcctc ggcgatgaac ggggcggtga tcgaaaccac gttcaacatc ttcttcaaac
      541 tgcaattcgg caaggtgtac gaagggattc tcaccactcc catgcaacct atggacatcg
      601 ccatcggaga gatcggatgg gcactgctcc gcgggcttct ctactcgacc gggttcatag
      661 tcatcatgtt cctcatgggg atgacggagt cgtggaccgc cattttcgcc gtgccctcag
      721 cgaccctcgt cggctttgcc ttcggcgccg tcggcctcgc cgcggccacc tggatgaagt
      781 cgtggcagga cttcgatctg gtgactctgg tgaccatgcc cctgttcctc ttctccggga
      841 cgttctaccc gttggacgta taccccccgg tcctgcagac ggtcgccaag ttctcaccgc
      901 tgtatcacgg cacggaactg ctccggggct tcacgctggg cccgatcgat tggacgatga
      961 tcggccacgc cgccttcctc gtcgtcatgg gtctcgtcgg cgcctccatc gccgcccgtc
     1021 gtctcgacgg catgctccgg aagtagtcaa gcgtgtgcag gtagcggggc cagagcacgg
     1081 catctgcaca cgcttatagg ctctcaccac atgctcgacc tcgccttccc tctccacgcc
     1141 ccacgcgggt ccgaactgaa caccaagggg tggctgcagg aaggcgccct gcgctgtctc
     1201 ctcaacaacc tggaccccga cgtcgcggaa gatccggaga atctcgtcgt ctatggcgga
     1261 cgcgggaagg ccgcccgcaa catcgacgcc ctccacgcca tcgtccgcac cctggaacgt
     1321 ctcgagaacg acgagacgat gctcgtccag tccggcaagc cggtgggagt cttccggacc
     1381 catgacctgg cgccacgtgt gctcatagca aatagcctgc tggtccccag atgggcgacc
     1441 ctcgaccact tctgggatct ggaggccaag ggtctgatga tgtatggcca gatgacggca
     1501 ggctcctgga tctacatcgg gacccagggg atccttcagg ggacctatca gaccttcgtt
     1561 gccatcgcgg agaagcggtt tgggggaacc ctcgccggca ctctcactct caccggtggc
     1621 ctgggaggta tgggtggtgc ccaaccactc gccatcacca tgaatggcgg ggttgcgctc
     1681 tgcgtcgagg tagatccgca tcgaatcgag cggcgtctgg agaccggcta tctcgacgtt
     1741 caggcagact cggtggaaca cgccatcgag ctggcgaccg aggccaaggc ggccaagaag
     1801 gccctttcga tcgggttgct cggcaatgcc gccgagttgt tcccccagct tctcgagtcc
     1861 gatctcgacg tcgacatcgt caccgatcag acctcggctc acgatcctct ctacggctac
     1921 atcccgagtg gatacaccct cgaggaggct gcttcgctgc gccaacacga ccctgacacg
     1981 tatatcaagg cggcccggga gtcgatggcc acccagtgtg aggcgatggt cgggtttcag
     2041 agggcaggag ccgaggtctt cgattacggc aacaacctcc gtggcgaagc ccagaccgcc
     2101 gggttctccg atgcgttcgc ctaccccgga ttcgtccccg cctacattcg agacctgttc
     2161 gcagagggct cggggccgtt ccgatgggtc gccctcagcg gggaacccgc ggacatcgag
     2221 accaccgacc gggctctggt cgagctgttt ccggacaacg agccgctcct ccgctggctg
     2281 cgcctcgcgg gagagagggt gcactaccag ggtctgcccg cccgaatctg ctggctgtcg
     2341 tacggacaga gacatctcgc cggattgcga ttcaacgagc tggtggccaa cggggaggtc
     2401 tccgcgccga tcgtgatcgg ccgtgaccat ctcgattcgg gctcggttgc ctcacccttt
     2461 cgggagacag aggccatggc cgatggctcc gacgccgtcg ccgactggcc gatcctcaac
     2521 gccatgctca atgtgtcgtc gggcgcggcc tgggtcgccg tccaccatgg aggcggcgtc
     2581 gggatcggca actcgatcca tgccggggcc caggtggtcg cagatggaac agatcagggc
     2641 gcgtttcggg tcgagcgcgt gctcaccaac gaccccggca ccggtgtgat gcgtcatgcc
     2701 gacgccggct acgagaaggc gcagcgagtg gcgcgcgagc gtggtgtcga catcccgatg
     2761 ctcgatgaag gtgattgacc cccactggcc ccgggccggg gtctggctgg ccagtgacgc
     2821 ccccgagccc gatctggtgg tggtcggtgt ccccaactcg cggtcgagtc tcactcactc
     2881 cgatgcagcc ctggcccccc tgaggatgcg cgagcgtctc gatcgcttct ccacgtttca
     2941 cggagagacg gagaccgatc tgtctgcggt gaaggtgagc gactttggca actggccggt
     3001 ctccgagatc gacccgatcg cactcatcgg cgagatccaa cagtgggctg cggagctgcc
     3061 cgatgtcgcc ctccggatct atctgggcgg cgacaacgcg atcacacgtc cccttgtggc
     3121 ggcgcagtct gatgaaccgg agcgcatagg gttgatcaca ttcgacgccc accacgacgt
     3181 gcgaacactc gagaccggcc cgagcaatgg caacccgatc agggggttga tcgaagagca
     3241 tggcctgccg ggccggaaca tcgtccagat cggcatccac tccttcgcca actcgaatgc
     3301 ctatcgggag tactgcgagc aagagggggt caccactgtg acggtcgccc agatcgagaa
     3361 catcgggatg gaagaagcgg tagacgtcgc tctcagccgg ctcagtgcaa cgtgtgatcg
     3421 gatctatgtc gacgtcgaca tcgacgtcct cgaccgcgcc tatgccccgg cgtgctcggg
     3481 agcccgaccg ggtgggctga gcgtccgtca gctggcgcac ggagtcactc gatgtgcctc
     3541 ccacccgatc gtgcgtgcga tcgacttcgt cgaggtcgat ccgtctgctg accgcgatga
     3601 tcagaccatt gacgtgatgg cccatctctt cctctccgcc gtggccggcc ttgcaaccca
     3661 acccggctag cccccgctga ccaactggtc tgcctcgggg gcgagatgat gggtggagcg
     3721 gtcgatgagg aagtcatgag ggagacggac cgggcggggg ccgtggctgt gaccacggac
     3781 catgcggttg gctgcctcgg ggaaggcgac gtcggaatcg agcttcccaa ccagatcggc
     3841 caactcgtct accgatgaga cccgcgacat ggcatccctg atcgccggac ctgccggaaa
     3901 acccttcaga taccaggatg ggtgcttgcg gaagagtcgg atgccgaacc tctccccgaa
     3961 atggtcgacg aggagttggg catggcggat catgatctga ccgacttcgc cgaggcttgg
     4021 aggctcttga agctggcgtc ccgcaaaccc gtcggcgagg tcgcggaaga gccagggacg
     4081 acccaagcat ccacgaccca ccacgacgcc ggcgcaaccc gtggactcca tcatccggac
     4141 tgcgtcatcg gcggtccaga tgtcgccatt tccgaggacc gggatcgact ggaccgcttc
     4201 gcggagacga gcgatggccg tccagtccgc ttcgcccgaa tagagctggc gggcggtgcg
     4261 ggcgtggagg gccaccgcag ccgctccctc gtcctcggcg acgagaccgg tgtcgatgta
     4321 agtcaggtgc tcgtcgtcga tccccttgcg gaacttgaca gtgaccggga cgtctccggc
     4381 gttggagacc gcggcgcgga cgatcgagcg aaagagatcg gtcttccagg gaagtgccgc
     4441 cccgccacca tgacgggtga ctttgcccat cgggcatcca aagttcatgt cgatgtggtg
     4501 aacgccctgt tcctccacca ggaatgacac ggcttcgccc agcgcaccgg ggtcggtgcc
     4561 atagagctga atcgagcgag ggacttcgtc ctcgtcgaac cgggccaggg cgagcgtctt
     4621 ctcattcctc tccaccagac cgcgagcgcc gatcatctcc gacacgtaca gaccggctcc
     4681 gaactcgcga cacagtctgc ggaagggcgc gttggtgacg ccggccatgg gcgcaaggac
     4741 cacgggcggg tcgacgcgaa gagagccgat ggagagggtc atgaagggaa gatgatattt
     4801 gcgtggaact gccaatcccc accgggacgt cgaaaccgca gatgagaagg gctctgatcg
     4861 cgggcttgcc gcttgccttg ctgctcgccg catgtggcgg gatcgggccc ggcacggagc
     4921 cggcccggac ctctcagagc ccgggcacat cggctccccc cgtcaccaca ccggccacgc
     4981 ccccaccgac caccggggcc ttgctccgct gtgatcaggt tccgctactc accgcagacc
     5041 cctcttacta ccgggacaca cccacgtaca taggcaacga gatgccggtc gacgaggtgc
     5101 gacggtgggc cgctcagtat gcggacttcg tggacatctg gatcgatcgt gaccacaacg
     5161 gttgggtcac ggtggcattc accgaggacg tggctgaccg acaggccgag atcgaacggg
     5221 agtttcctga agatggcgtc gtcgcccttc agatcgagct gaccgaagaa gagctctcga
     5281 gtttgcagaa agaagtcgtc gaggggctgg acggagtggt cgacgtcctc ggcgcatgga
     5341 ccgacgtgat caggggccat gtcaacgtcc agatcccggt tctcagtgaa gagaacctct
     5401 cggccatcga agctctgttc cctgatgccc cgatctgcgt ggaagggctc ccacccgaga
     5461 tgttcgtcga gcctggcccc caacccgagt cgggcgacgg ttggcagctc atctacgagc
     5521 agaagcatgc cggattcccc tatgtgaccg ggctggcctg ggatcaggag agctttcacg
     5581 ccctcctgga tgacatcgaa ggcatcgccg agctcgatgt cgacgtcgac ttcgagaacc
     5641 aggtcgtgat ctggttcgga gctgtcttcg gcagcagctg tcccaacatc cggatggatg
     5701 acgtggtgat cgatggcgac accatctacc cgttgatcgt cgacaccgac aactcctttg
     5761 cctgcacctc ggacgccaac ccacacacct acctcgtcgg tctcgaccgc gaccggctgc
     5821 cggccccgcc gttctacgtc cagatctacc ccgacttgga cttcgatcga gtgctcgtcg
     5881 aagcggacct gcggccggcg gggtccacgg cgttgcccgg tgaggtgggg ccggacccca
     5941 acccgcccgg ccctaagccg gagcgctccg gagccatcat cgagccggga tatccgtggc
     6001 agtacgagat cgacctgacg tgcggctggg agtggctcgg tgagatcaac tcatatcagt
     6061 gggtagccac tggcccgatc ccagagtcat ggctggcgaa gacagacggg gagtccgccg
     6121 tgacggttga agtcacgctc aacgaaggcg gcccggaccc gttcgtcgaa gtcgtcttcg
     6181 acgggcaaag cgtgatctac gagatcggga gatcgcctgg ttgctgagct tcagttgacc
     6241 acggtggggt tggcgcgccc ggactgcgct acatcgacca ggttgttgag gaatcccacg
     6301 cgggccgggg tgatgacggc gattatcgga tcgtttggct cgaagccccc gtgggtgaag
     6361 agcgacgac
//
LOCUS       DASUTA010000378         2607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1276956, whole genome shotgun sequence.
ACCESSION   DASUTA010000378 DASUTA010000000
VERSION     DASUTA010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276956"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VFZ80_07550"
     CDS             <1..603
                     /locus_tag="VFZ80_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6287325.1"
                     /translation="EEIVQRILSSVGKVDSMKLRSGQLGPLWQRVVDAAGRMYKAPIY
                     IDDSPVVTVTDIRAKCRRLKRKKGLSLVVVDYLQLMEAHGPENRQQEIAQISRNLKNL
                     ARELEVPIIAASQLNRSLESREDKRPRLSDLRESGSIEQDSDVVMFVYRHEYYHPEDQ
                     DKKGIAEIIVAKHRAGSTGPVEMTFQPEFTRFANLGRDVT"
     gene            complement(788..1351)
                     /gene="dcd"
                     /locus_tag="VFZ80_07555"
     CDS             complement(788..1351)
                     /gene="dcd"
                     /locus_tag="VFZ80_07555"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX6287326.1"
                     /translation="MIFSDRSIKEAIDAGTIQIDPYEPSYVQPSSVDLRVGDGFRIFA
                     NHKYSEIDPRSPQDDLTQLVEVGEGPFMLHPGEFVLGSTLERVKLGEDVVARLEGKSS
                     LGRLGLLIHSTAGFIDPGFEGHITLELSNVATLPIAIYPGMKIGQISFYQMTTAADHP
                     YGSPELGSKYQGQSGPTASRSHRDFDN"
     gene            complement(1497..1567)
                     /locus_tag="VFZ80_07560"
     tRNA            complement(1497..1567)
                     /locus_tag="VFZ80_07560"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1533..1535),aa:Gly,seq:ccc)
     gene            1685..>2607
                     /locus_tag="VFZ80_07565"
     CDS             1685..>2607
                     /locus_tag="VFZ80_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6287327.1"
                     /translation="MTNGAFRAFVLRWGDVAWRLVAMGVVVFFAFRILKTVSVVVFAV
                     VFALFLAAVLWRPSQWLMQRGWKPALASMATIMVAALILAGVIVFIIPQVVDSFDTLS
                     QDVNETWESFHDWLIDGPLGLTQQDLDDYTERIAGWLQGIGGDRLIGGATAILEFITG
                     AFLVIVITFFMLKDGRLLLEKALDRTPDETASKIAIGAQVARDSLAKYMGGIALVGLF
                     DATLIAIALWIVGAPLVLPLALLVFFGAYIPLVGAFVSGLLAVAVTFVNVGSIQALII
                     LAVVIIVQQFEGDVIMPLVFGSRLRLHPLVVL"
BASE COUNT          472 a          781 c          832 g          522 t
ORIGIN      
        1 gaggagatcg tgcaacgcat cctctcgtcg gtgggcaagg tcgactcgat gaagctccgc
       61 tccggccagc tcggccctct ctggcagcgg gtggtcgacg ccgccggccg gatgtacaaa
      121 gcacccatct acatcgacga ctcaccggtg gtcacggtca ccgacatccg ggcgaagtgc
      181 agacggctca agaggaagaa gggcctcagt ctcgtcgttg tcgactatct gcagctgatg
      241 gaggcccatg ggcccgagaa ccgccagcag gaaatcgccc agatttcgag gaacctgaag
      301 aacctggcgc gggaactgga ggtcccgatc atcgcagcct cccagttgaa ccgatcgctg
      361 gagtcgagag aagacaagag gccgcgtctg tccgaccttc gtgaatctgg ttcgatcgag
      421 caggactcag acgtggtgat gttcgtctat cgacacgagt actaccaccc cgaggaccag
      481 gacaagaagg gcatcgccga gatcatcgtg gccaagcatc gcgccggctc gacgggcccg
      541 gtggagatga ccttccagcc cgagttcacc cggttcgcca acctgggccg cgacgtcacc
      601 tgatcggcaa agaggatgtg cggcagcccg gtggctgccg cggaggggtt tgtcacttgc
      661 cctgtgaagc ctggaaagct tcgagttggg gactcaaatc gggcaacgga ccaacgctgt
      721 cagtctcgcg gatgtgcggg atctcgtgtt gtcgatgccc ggaccaatcc gaatacacac
      781 attcgcacta gttatcgaaa tcgcgatggg agcgactggc cgtcggcccc gattggccct
      841 gatacttcga gcccagctcc ggtgagccgt aagggtgatc ggccgccgtg gtcatctgat
      901 agaagctgat ctggccgatc ttcatccctg ggtagatcgc aattgggagt gtggcgacgt
      961 tggagagctc cagggtgatg tgaccctcga agccgggatc gatgaaccct gcagtggagt
     1021 ggatgaggag accgagacga cccagggagg acttgccctc caatcgggcc acgacatcct
     1081 caccgagctt gacccgctcc agcgtcgagc ccaagacgaa ctccccgggg tggagcatga
     1141 atggcccctc tcccacctcg acgagttggg tgaggtcatc ttggggagac ctcgggtcga
     1201 tctcggagta cttgtggttg gcaaagatgc ggaacccgtc gccgactcgg aggtccaccg
     1261 aggaaggctg gacataggaa ggctcgtagg gatcgatctg gatcgtgccc gcgtcgatgg
     1321 cctccttgat cgatctatca gagaagatca ttggcgaggc atggtaggcg gcctcgagcg
     1381 tgtgcaggaa gtgtgacgtg gccacggctc ctgcacacgc tcactgagcg ggtgacggga
     1441 atcgaacctg cgtcttgagc cgttgggcac ggcctcacgt ttgaacgtga gctctgagcg
     1501 ggtgacggga atcgaacccg cgtcttgagc ttgggaagct cacgttctgc cattgaacta
     1561 cacccgcgag gaccatcaga gtagtcaggg tgggggtgaa ggaatctcgg caagtcgggc
     1621 cgtgttccct cgggaccgtt cccgggtatg ggacactcga cctactggaa agggagtccg
     1681 caccatgacc aacggcgcct tccgcgcttt cgtcctccga tggggggacg tcgcctggcg
     1741 cctcgtggcc atgggggttg tggtcttctt cgcctttcgg atcctcaaga cggtcagtgt
     1801 cgtcgtcttt gcggtggtct tcgccctgtt cctggcggct gtgttgtggc ggccatcgca
     1861 atggctgatg caacgtggct ggaagcccgc cctcgccagc atggcgacga tcatggttgc
     1921 cgcgctcatc ttggccggtg tcatcgtctt catcattccc caggtcgtcg acagcttcga
     1981 tacgctgagc caggacgtca acgagacctg ggagtcgttc cacgactggc tgatcgacgg
     2041 ccccttgggc ctcacccaac aagatctcga cgactacacc gagcgcatcg ccgggtggct
     2101 ccagggaatc ggaggcgatc gcctcatagg aggcgccacc gcgatcctcg agttcataac
     2161 cggcgccttc ctcgtgatcg tcatcacctt cttcatgctc aaggatggcc ggctcctgtt
     2221 ggagaaggcg ttggatcgaa cgcccgacga gaccgcgagc aagatcgcga tcggagccca
     2281 agtggccagg gactcgctgg ccaagtatat gggaggcatt gccctggtcg gcctgttcga
     2341 cgccacgttg atcgccattg ctctctggat agtcggtgcc ccgctcgtgt tgcctctcgc
     2401 cctcctcgtg ttcttcggtg cgtacatacc gttggtgggt gcgttcgtct ccggcctgct
     2461 cgccgtcgcc gtcaccttcg tcaacgtcgg ctcgatccag gcgctgatca tcctcgcggt
     2521 cgtgatcatc gttcagcagt tcgaggggga tgtgatcatg cccctggtct tcggctcccg
     2581 gttgcgtctc cacccgttgg tcgtcct
//
LOCUS       DASUTA010000379         2199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_112232, whole genome shotgun sequence.
ACCESSION   DASUTA010000379 DASUTA010000000
VERSION     DASUTA010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112232"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VFZ80_07570"
     CDS             <1..809
                     /locus_tag="VFZ80_07570"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEX6287328.1"
                     /translation="GGWIGSPVADGSDLSVLFLHNDGFSTMCGHGIIAMGKVLIETGM
                     IDAVEPETSIVIDTPAGTVRAVSSVRSGTVSATRFRNVPSFVADLDASVEVEGLGPVR
                     YDLAFGGAFYALVNATDIGVSLDDAAGLADAGRRVKRAVTESRDIAHPDHPDLGFLYG
                     VIFTGPAESPSNHSRNVCVFADGEIDRSPTGTGVSCRLAVMSARGEIGVDEEITIESI
                     VGSTFTGRIVERSDSPATIVPEVSGTAHITGRSEYWLDPEDDLGGGFLLR"
     gene            complement(806..>2199)
                     /locus_tag="VFZ80_07575"
     CDS             complement(806..>2199)
                     /locus_tag="VFZ80_07575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6287329.1"
                     /translation="FFLAAYGAIIVAVYRLARLDSGGREWVLTILDAAVGGIALAALV
                     WSALFADLMATLEGAPWWEIAIAASYPILDVMVVVGLIIMIMRRSNYHFDPRLVLVAI
                     GGGAQVLADLIYLRRSIGEQFAAAEPAYALNLLAVAFLLTAATIVDRSARRREFAEAP
                     TPIWVLMWPYVIAGVLLVVHFKNYRDLAPGSSEILLLDAVILIGVVILLRQVYVIYRD
                     RNRVDRKRAELVASVSHELRTPLTAMVGFLTLLDEHADEFPADARNEMLSEAAVQARH
                     MSRLVSDLLVLARGDTSLMSMERKEVPAMSIVLSVLRGTDTGNVRIEEDLDASVQVHV
                     DPDRMKQALGNLLNNAIRYGGDRCLVVAKVMGRDLVFEVHDNGEGVPARFEAAIWQQF
                     ERGAHRLDAVTPGLGIGLSIVQAIMEAHGGRAEYRQSERLGGACFSLSIPGSVLEIER
                     LDRRVRSVVNRGG"
BASE COUNT          416 a          707 c          700 g          376 t
ORIGIN      
        1 atggaggatg gatcgggtcg ccagtcgccg acgggtccga tctgtcggtt ctcttcctcc
       61 acaacgacgg cttctccacg atgtgcggtc acgggatcat cgccatgggc aaggtgttga
      121 tcgagaccgg gatgatcgac gcagtcgagc cggagacctc catcgtcatc gatactccgg
      181 cggggactgt ccgggccgtg tcctcggtga ggtcgggcac cgtgtcggcg acgcgtttcc
      241 gcaacgttcc ctcctttgtt gccgacttgg acgcctcggt cgaggtcgaa ggtcttggcc
      301 ccgtgcgata cgacctggcg tttggtggag ccttttacgc acttgtgaat gcgaccgata
      361 tcggtgtttc gctcgatgat gcagcaggcc tggcagacgc cggccgccgc gtgaagcgtg
      421 cggtcaccga gagccgtgac atcgcccatc cggaccaccc ggacctgggg tttctatacg
      481 gcgtgatctt cacgggcccg gctgagagcc cgtcgaatca cagcaggaat gtgtgcgtct
      541 tcgccgacgg cgagatcgac cgctccccaa cgggcaccgg ggtcagctgt cgtctcgcgg
      601 tgatgtcggc gcgcggggag atcggtgtcg acgaggagat aaccatcgag agcatcgtcg
      661 gaagcacatt cacggggagg atcgtggaga ggtcggacag cccggcgacg atcgtccccg
      721 aagtcagcgg caccgcgcac atcacgggcc gctccgagta ctggttggac cccgaggacg
      781 atctgggagg cgggttcctc ctccgttagc cgccccggtt gacgaccgac cggacccgac
      841 gatcgagcct ctcgatctcg aggacgctcc ccgggatcga cagtgagaag catgcgcccc
      901 cgagcctctc agattgccgg tactcggccc ttccaccgtg tgcctccatg atcgcctgca
      961 cgatggacag cccgattccc agcccaggtg tgacggcgtc gagacgatga gcccccctct
     1021 cgaactgttg ccagatcgca gcctcgaacc gggctggcac accctccccg ttgtcatgga
     1081 cttcgaagac caggtcacgg cccatgacct tggccacgac caggcaccga tcgcctccgt
     1141 agcgaatcgc gttgttgagg aggttgccca acgcctgctt catacgatcg gggtctacgt
     1201 gaacctgcac cgaggcatcg aggtcctctt cgatgcggac gtttccggta tccgttcccc
     1261 gcaacaccga gaggacgatc gacatcgccg ggacctcctt gcgctccatc gacatcaggc
     1321 tcgtgtcccc ccgagccagc acgagcagat cggagacgag acgtgacata tgacgagctt
     1381 ggaccgcagc ctcggagagc atctcattgc gggcgtcggc tggaaactcg tctgcgtgct
     1441 cgtcgagcag tgtcaggaat cccaccatgg ctgtgagcgg ggtacgcagc tcgtgactca
     1501 ccgatgccac cagctcggca cgcttccgat cgactcggtt gcgatcgcgg tagatcacat
     1561 agacctggcg cagcaggatc accaccccga tcagaatcac tgcgtccaac aggaggatct
     1621 cgctcgaccc gggggcgaga tcgcggtagt tcttgaaatg gaccaccagg aggacaccgg
     1681 cgatcacgta gggccacatc aggacccaga tcggcgtcgg cgcctcggcg aactcccgac
     1741 gacgagcact gcggtccacg atcgtcgccg cggtcagcag gaaggccacg gcgagcaggt
     1801 tcagagcata tgcgggctcg gcggcggcaa actgctcacc gatgcttctc ctgagataga
     1861 tgagatcggc gaggacctgg gcgcccccgc cgatggcgac caggacgaga cgggggtcga
     1921 agtgatagtt cgaccggcgc atgatcatga tgatcagtcc cacgaccacc atcacgtcga
     1981 gaatgggata gctggcggcg atagcgatct cccaccacgg tgccccttcc agggttgcca
     2041 tcagatcggc gaatagggcg ctccagacga gagcggccag cgcaatcccg ccaaccgcgg
     2101 cgtcgaggat ggtgagcacc cactcgcgtc ctccgctgtc gagacgggcc agccgataga
     2161 cggcgacgat gattgcgcca taggcagcca ggaagaaac
//
LOCUS       DASUTA010000380         2393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1407965, whole genome shotgun sequence.
ACCESSION   DASUTA010000380 DASUTA010000000
VERSION     DASUTA010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407965"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..919
                     /locus_tag="VFZ80_07580"
     CDS             107..919
                     /locus_tag="VFZ80_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6287330.1"
                     /translation="MQRRADRYGPKEYDHLLEVRDIHLSFKGVKAVDGVSFTVDDGEL
                     FAIIGPNGAGKTSLFNSINQVYHPQQGDILWKGESLMGLKPNVTAERGIARTFQNIAL
                     YPHMTVLENILTGRHVRMGAGWIAGALWIGKAKSEEMENREIVESIIDFLEIEQWRKY
                     PVGLLPYGIQKRVELGRALAMDPELLMLDEPVAGMNLEETEDMARFILDVQDELRIAI
                     ILVEHDMGLVMDIADRVLVLDFGQQIATGKPAEIQANPNVIKAYLGEELETS"
     gene            complement(1042..1611)
                     /locus_tag="VFZ80_07585"
     CDS             complement(1042..1611)
                     /locus_tag="VFZ80_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEX6287331.1"
                     /translation="MTSAVISQRAFPRSGIVSVILVVAAAALTALAAQWRIQLPFTPV
                     PITGQTFAVLLTGAALGWKLGAAGQLLYLAVGVLGAPVFADGESGVDYIQGATGGYLI
                     GFVFAAGLVGWMAEHRQDRTFATMFTAFILGSAVIYVFGVIGLMIATDWTLSEAVARG
                     VVPFLLGDLIKAAAAGLLLPGAWRLLGDR"
     gene            1718..>2393
                     /locus_tag="VFZ80_07590"
     CDS             1718..>2393
                     /locus_tag="VFZ80_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX6287332.1"
                     /translation="MEAETIKKSNASWPLYLIGALFFVGVLGIAAWNVELPYLAYSAG
                     PVSDAADTVVAEEVPVYPPDGELLMLTVVSQDVNVFEALIAGVDPTIDLVRKQAVRRE
                     GETDEEYRNRVLQQMDDSNFRAIAVALQHLGYEMVPTEVVINDIAENVPAADVLERGD
                     TVLSVNGVPIETVDDFAPALEGYQVGDTITMTLLRNGTEVEVDVVLAEKEAEPGEPMI
                     GVFLGEL"
BASE COUNT          497 a          714 c          766 g          416 t
ORIGIN      
        1 aagcggagga aacgttggct gtcgtgaggg gtctcttcgc tcgcggtatg aagtatgaag
       61 tatcgagtac gaagactcgg gcggtgttga attgagctcg aagctcttgc agcgacgggc
      121 ggatcgctac gggccgaaag agtatgacca cctgctagag gtgagggaca tccacctgtc
      181 gttcaagggc gtcaaagcgg tcgacggggt ctcgttcacc gtggacgacg gtgagctgtt
      241 cgccatcatc gggcccaacg gagccggcaa gacctccctc ttcaactcga tcaaccaggt
      301 gtatcacccc cagcagggcg acatcctctg gaagggggag tcgctgatgg ggctgaagcc
      361 caacgtcact gccgagcgag ggatcgcccg gacgttccag aacatcgccc tctacccgca
      421 catgaccgtg ctggagaaca ttctcaccgg tcgacacgtg cggatgggag cgggatggat
      481 cgcaggtgcg ctctggatcg gcaaggccaa gagtgaggag atggagaacc gcgagatcgt
      541 cgagtcgatc atcgacttcc tcgaaatcga gcagtggcgc aagtacccgg tagggctgct
      601 tccctatggc atccagaagc gagtcgagct gggccgagca ctggccatgg atcccgagct
      661 cctgatgctc gacgagccgg tagctgggat gaacctcgag gagaccgagg acatggcccg
      721 attcatcctc gacgtccagg acgagctgcg gatcgccatc atcctcgtcg agcacgacat
      781 gggcctggtc atggacatcg cagaccgcgt gctggtcctc gacttcggcc agcagatcgc
      841 aaccggcaaa cccgcagaga tccaggccaa tcccaatgtg atcaaggcct acctcggcga
      901 agagctggaa acctcctgat acccgaaccc ggggttcctg cgtgtccttc ggaacgttgg
      961 agtttcctgg ggaaactcgc tacctgtcga acttcgttcg acaggtagcc ggatacggcc
     1021 caaatcggct caactacgtg atcaacggtc cccgaggagt cgccaggcgc ctggtaggag
     1081 tagccccgct gctgccgcct tgatcagatc accgaggagg aaggggacga ctccgcgagc
     1141 gacggcctcg gagagcgtcc aatcggtggc gatcatcaga ccgatcaccc cgaatacgta
     1201 gataactgcc gagccgagga tgaacgcggt gaacatggtg gcaaaggtcc gatcctggcg
     1261 atgctccgcc atccagccga cgagacccgc ggcgaagacg aagccgatga ggtagccacc
     1321 ggtggctccc tggatgtagt cgacaccgct ctccccgtcg gcgaagacag gggcgccgag
     1381 gacgccgacg gccagataga gcaattgccc ggctgcaccg agcttccagc ccaacgcagc
     1441 gccggtcaac aggacggcga atgtctgccc ggttatcggg accggggtga agggcagctg
     1501 gatccgccat tgggcggcca gggcggttag ggctgctgcc gccaccacca ggatgaccga
     1561 cacgatcccg gagcggggga aggctctttg gctgattact gcggacgtca cgttcaactc
     1621 ctcgaaaccg tttgccgttg agaatacgtc tcaggtgctc acagccacgg gtagcactga
     1681 gacccgtagt gccatgtcat cgttactctc gccgcccatg gaagccgaaa ccatcaagaa
     1741 atccaacgct tcctggcccc tctacctgat cggggccctg ttcttcgtgg gcgtgctggg
     1801 aatcgccgcc tggaacgtcg agcttcccta tctcgcctac tcggcgggcc ccgtttctga
     1861 tgccgccgac acggtcgttg ccgaagaagt gcctgtgtac ccgcccgatg gcgagctgct
     1921 catgctgact gtggtgagcc aggacgtgaa tgtcttcgaa gcgctcatcg ccggagtcga
     1981 tcccaccatc gatctggtga ggaagcaggc ggtgcgccgg gaaggtgaga ccgacgagga
     2041 atatcgcaac cgtgtcctcc agcagatgga cgactccaac ttccgggcga tagcggttgc
     2101 actgcagcat ctcggatatg agatggttcc cacggaggta gtcatcaacg acatcgccga
     2161 gaacgtcccc gctgccgacg tcttggagag gggcgacacg gttctcagcg tcaacggagt
     2221 gccgatcgag actgtcgacg acttcgcccc agctctggag gggtaccagg tgggggacac
     2281 catcaccatg accctcttgc gcaatggcac ggaggtcgaa gtcgatgtgg tgcttgcaga
     2341 gaaggaagcc gagcccggcg agccgatgat cggcgtcttc ctgggggagt tga
//
LOCUS       DASUTA010000381         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_499109, whole genome shotgun sequence.
ACCESSION   DASUTA010000381 DASUTA010000000
VERSION     DASUTA010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499109"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VFZ80_07595"
     CDS             complement(<1..696)
                     /locus_tag="VFZ80_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287333.1"
                     /translation="MSSTYTSGSRVDSARQTLRKILRNARDKEKWRKVVKFRLWMPVA
                     LQIILIIGLLWYTSTRFEGFLNSTNIFNILILALPLMVAAMAQTHAILVGYLDLSVGA
                     MISFGVVLASFMIGAEASLSQILVGSAIVLLAGVGLGLANAGLIRGVKIPSIIATLAT
                     LSILDGISLTLRPTAQGIISGDLVGFLTTTVGPIPIAFFVILAGAGALDLWLHASGSG
                     LQVRAVGFDETAAK"
     gene            complement(693..>2126)
                     /locus_tag="VFZ80_07600"
     CDS             complement(693..>2126)
                     /locus_tag="VFZ80_07600"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX6287334.1"
                     /translation="KSTLLGIASGFVDPDLGEIRIGGERLRTDSPAQARRLGLAMAYQ
                     DGSSVGMEPVKNNLYFAAPPEQRPPYWRRKKWARKLLADFDLDIELFPDVPAGFLTMA
                     DRQLFEVAKALVTDPKVLLLDEPTTALGPDEVEALHRTILSCKERGVGVVYVSHRLPE
                     VLEVADRVTVLRDGRYQGTFDARTTTEHELVERIVGRPFEAAFPEPKHVEGEREEVLV
                     VDGLQGQSFGPVSFTLDRGEIVGIAGAEGNGQSQLFDTLAGRTPPRAGLVKCRDKELT
                     LISTHETVEAGIMLLPGNRKTEALMGVLGVRVNSTVQSLRRFSALGLLRRVHERNKVG
                     ELVDELEIRTPSLDQPVEFLSGGNQQKVAVARSFLEEPHVILAYEPTQGVDVGSRFDI
                     YKALKARSDAGAAILAKSSDPIELAGLCDRVLVMSRGTIVEEIPGDELDEVRIVEAIV
                     RGPGMSKAARSPLGVAMPKTASAAETS"
BASE COUNT          404 a          667 c          677 g          378 t
ORIGIN      
        1 tttcgccgcg gtctcgtcga acccgacggc ccgcacctga agaccagacc cggaagcgtg
       61 aagccacaga tcgagggcgc cagccccggc caagatcacg aagaaggcga tcggaatcgg
      121 gccgaccgtc gtggtgagaa atccgacgag gtcgccgctg atgattcctt gagcggtggg
      181 ccgcagagtg agagagatcc cgtccaggat ggagagggtg gcgagggtgg cgatgatgga
      241 agggatcttc accccccgga tgagcccggc gttggccaaa ccgagcccga ccccggcgag
      301 gaggacgatg gcgctgccca cgaggatctg agagagcgag gcttcggcac caatcatgaa
      361 tgaggcgagc acgacgccga agctgatcat cgctcccacg gagagatcga ggtatcccac
      421 gaggatggcg tgggtctggg ccatggcggc caccatcaga ggcagagcca ggatcaggat
      481 gttgaagatg ttcgtggagt tgaggaagcc ctcgaagcgg gtggacgtgt accagagcaa
      541 cccgatgatg aggatgatct gaagtgccac cggcatccag agccggaact tgacgacctt
      601 gcgccacttc tccttgtctc gcgcgttgcg aaggatcttc cgcaacgtct ggcgagcact
      661 gtccaccctc gatccggagg tgtaagtgct gctcatgatg tctccgcagc cgaggccgtc
      721 ttgggcatgg cgactccgag aggggaccgt gccgcctttg acatcccagg gccgcggact
      781 attgcctcga cgatccgaac ctcgtccagc tcgtctccgg ggatctcttc gacgatcgtc
      841 cccctggaca tcaccaggac acggtcgcac aggccggcga gctcgatcgg gtcgctggac
      901 ttggccagga tcgcggctcc ggcgtcactc ctggccttta atgctttgta gatgtcgaaa
      961 cgcgatccga catcgactcc ctgggtcggc tcataggcga gaatcacatg tggttcctcg
     1021 agaaacgacc gagccaccgc gaccttctgt tggttcccgc ccgagagaaa ctcgactggc
     1081 tgatccaaag aaggagtcct gatctcgagc tcgtcgacca actctccgac tttgttccgc
     1141 tcatggacgc ggcggagcaa gcccagcgcg gagaagcgtc gcagggactg gaccgtcgag
     1201 ttgaccctga ctccaaggac ccccatcaag gcttcggtct tacgattgcc gggaaggagc
     1261 atgatcccgg cctcaaccgt ctcgtgagtc gagatgaggg tgagctcctt gtctctgcac
     1321 ttcaccagtc cggcgcgcgg tggcgtccga ccggccagtg tgtcgaagag ctgcgactgt
     1381 ccgtttccct cggctccggc gataccgacg atctcgcctc tgtccagagt gaagctgacc
     1441 ggaccgaaag actgaccctg caagccgtcg accaccagga cctcttccct ttcgccttcg
     1501 acgtgtttcg gctccgggaa agcggcctcg aatgggcgcc cgacgatcct ctcgacgagc
     1561 tcgtgctccg ttgtcgtcct ggcatcgaag gtgccctgat agcgaccgtc ccggagcacc
     1621 gtcacccggt ccgcaacctc caacacctca ggaaggcggt ggctgacgta aacgacaccc
     1681 acaccccttt ccttgcagga gaggatggtg cggtgaagcg cttccacctc gtcgggcccg
     1741 agtgccgtgg tgggctcgtc gagcagcagc actttcggat cggtcaccaa tgccttggcc
     1801 acttcgaaga gctgtcgatc ggccatggtg agaaagccag cgggcacgtc ggggaagagc
     1861 tcgatgtcga ggtcgaagtc ggccaggagc ttgcgcgccc acttcttccg tcgccagtac
     1921 ggggggcgct gctctggcgg cgccgcgaag tagaggttgt tcttcaccgg ctccatgccc
     1981 accgaggagc catcctggta cgccattgcc aggccaagtc gtcgggcctg agccggggag
     2041 tcggtcctga gacgttctcc cccgatccgg atctcgccca ggtcgggatc gacgaaccct
     2101 gaggcgatcc cgaggagagt cgactt
//
LOCUS       DASUTA010000382         4056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_887452, whole genome shotgun sequence.
ACCESSION   DASUTA010000382 DASUTA010000000
VERSION     DASUTA010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887452"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            425..853
                     /locus_tag="VFZ80_07605"
     CDS             425..853
                     /locus_tag="VFZ80_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287335.1"
                     /translation="MSSVRRTRLALVLLAVALFLTACASESETVESPITEDQATEMAE
                     NALQGFNDRDYAAWSRDWSDTMKAAIDEDAFYAFRDQYHGELGDWVSINDLSGGPGAD
                     AGTYRWTYDLEFENGDYQMWFGFKEGSALIEGVTFEEPSS"
     gene            complement(960..1757)
                     /locus_tag="VFZ80_07610"
     CDS             complement(960..1757)
                     /locus_tag="VFZ80_07610"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6287336.1"
                     /translation="MVFEPAIGDVGLTWGLVQPAVSEHTSTFTHDRPGLGGSEPSDSP
                     RTVDVMADELRAALHATRFEPPYLLVGHSFASLTVQAFAHRHRAEVSGLVLVDGAHED
                     QMERFPEELDPRSMLAGVADQLRGLADSVRQGEAPPELIPVPGTFPETLAAAYREATS
                     PTPDRLEAAAAEYEGLEASQRQVRELSKSSLGDIPLIAIRHGVPQSMRGVADEINRRY
                     EETWQELQSELAGRSSSGRVVVADGAGHLIHHDRPDVVVDAILGLLR"
     gene            1887..3092
                     /locus_tag="VFZ80_07615"
     CDS             1887..3092
                     /locus_tag="VFZ80_07615"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6287337.1"
                     /translation="MVRRLQATELADEAGASLELIETLTQVGVLKPETDGSYGSGDLI
                     RLEAANAFLDAGVTLEQLDAALSSGMFTFEYLDRFHPEPGPPSGHTFAGFANSLGVSP
                     ELVGNIYLAMGLAQPRPDVVMRQEEVDLIRGFLEAWEIGGDEETFLRAARLVGEPARQ
                     VSAGWTRLFVEKVSDPLVAEDISVEQRISAVVGSTERLTKLAPPLLLWLFNRHLRHAI
                     DQANIDGMERELVAHGLVIPGPDQPTAIAFVDISGYTSMTEARGDELAAQAAERLRHL
                     AALATHSHSGTVVKLLGDGVMLHFDTVDAGLAGVFELVESLEASDLPAHAGIHAGPVI
                     EHDGDYYGRTVNLASRVAGVATAGTVLATGGVVSAVETGDYTFHPFPEVELKGVSEPV
                     SLYKATKVG"
     gene            3250..>4056
                     /locus_tag="VFZ80_07620"
     CDS             3250..>4056
                     /locus_tag="VFZ80_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX6287338.1"
                     /translation="MTHVSRDLLETISPSEWSVASPEQIIEAPADGVYPLLGSSPDGL
                     SIAEAELRLGEYGPNTLSPPARRPLISRFVSNFTHFMALLLWVGGAVAFAADLPELGV
                     AIWIVNLINGVFSFWQEYKAEKATEALLRLLPTTATVLRGGLPLEVVATDLVPGDVVI
                     LEEGDSISADGRLVEHVALRVDQSTLTGESHLVRKASDPVDPRGSGRLEIPNLVHAGT
                     TVASGRGKLVVTATGANTQLGAIAEMTGSLAETPSPLQRELKRLSVVVSVI"
BASE COUNT          747 a         1224 c         1300 g          785 t
ORIGIN      
        1 attccttcga cggggggagt ggtcgtcggt acggatctcg tggaggtcgt ctccgcgggt
       61 gtggtcgtgg tggacgcaac aggtgattcc gcacaggcgg tcgagatcgt caggaacaga
      121 gcggctgccg cgatgccgaa cctcttcctc gggcgatccg tcacgtcgct gttctttctg
      181 tcacgtcctc gaaggaaagg atcgacgaag acggccggca gggccagggc cgttggtcac
      241 cgaaaggaca gtccaacgtc aggttgctga cgattgacac aacatccgag acacggactg
      301 cattgagagt cttcaggccc tgagcacagg ccccctctat gagtcacgat ggctcccgtc
      361 caatcaccgg acgcgatact catccagatg caaaggagga atgatgaccc gtactgttga
      421 tcgcatgtcg tctgtgcggc gaacccgctt ggccttggtt ctgctcgccg tggccctgtt
      481 cctgactgcc tgcgcttcag agtcggagac ggtcgaatca ccgatcacag aggatcaggc
      541 cacggagatg gccgagaacg ctctccaggg gttcaacgac cgcgactatg ccgcttggtc
      601 gagggactgg tcagacacta tgaaagcagc catcgacgag gatgccttct acgcgtttcg
      661 ggaccaatac cacggtgaac tcggcgactg ggtctcgatc aacgatctca gcggtggacc
      721 gggcgcggat gcgggaacct accggtggac atacgacctc gagttcgaga acggcgacta
      781 ccagatgtgg ttcgggttca aggaggggtc ggcgctgatc gagggggtta cgttcgaaga
      841 gccgagcagc tgaaggctgc gggagccacc ctcgccttgc cttctgggcg gatccttgac
      901 cggaatccgg acacttatgc acccagaaag tgaaaggact ggctccgtgg aagccaccgt
      961 caacgcagca gtccgaggat ggcatccacg acaacatccg gacggtcgtg atgtatcaga
     1021 tgcccggctc cgtcggcgac gacgacccgt cccgacgatg accgccccgc cagctcactt
     1081 tgcagctcct gccacgtttc ctcgtatcgt cggttgatct catcggcgac accgcgcatg
     1141 gattggggga ccccgtggcg aatggcgatg agcggaatgt cacccagcga tgacttgctc
     1201 agttctcgaa cctgtctctg gctcgcctcc aacccttcgt actcggccgc cgccgcctcc
     1261 aggcggtcgg gtgttggtga agtggcttcc ctgtacgcag cagccagggt ctcagggaat
     1321 gtgccgggca cgggaatgag ctcgggcggc gcctcgccct gccggacaga gtcggcgagc
     1381 ccacggagct gatcggcgac tcctgccagc atcgaccttg gatcgagctc ttcggggaac
     1441 ctctccatct ggtcttcgtg ggcgccgtcc accagaacca gccctgacac ctcggcccgg
     1501 tgacggtgcg cgaacgcctg caccgtgagc gatgcgaagg agtgacccac gagcaggtat
     1561 ggcggctcga atcgggtggc gtgcagcgcg gcacgaagct cgtcggccat cacgtcgaca
     1621 gtccttggag aatcagatgg ttcactccca cccagaccgg gccggtcgtg agtgaacgtc
     1681 gacgtgtgtt cggagacagc gggctgtacc aggccccagg tcagcccgac gtcgcctatt
     1741 gccggctcga agaccaccgc cggggaacct tctccagcga cgtacacact atgcgatcga
     1801 ctgttcacag cgaccaggcg agaagggagt tggttcgtcg tcactcgttg gactctatgt
     1861 cgccacctca acctacgatc cggggcgtgg tgcgcagact gcaggcgaca gagctggccg
     1921 atgaggcggg tgccagtctc gagctcatcg agactctgac tcaggttggt gttctgaaac
     1981 cggaaacaga cggctcgtat ggatcggggg acctgatccg tctcgaggca gccaacgctt
     2041 tcctcgacgc cggcgtgacc ctcgagcagc tggacgcggc gctttcctcg ggcatgttca
     2101 cattcgagta cctcgatcgc tttcatcccg agccaggccc accgtccggc catacgttcg
     2161 ctgggttcgc gaacagcctc ggagtgtcac cggaactggt ggggaacatc tacctggcga
     2221 tgggtttggc tcagccgaga cctgacgtgg tcatgcggca agaggaagtc gatctcatca
     2281 ggggttttct ggaggcgtgg gagattggtg gcgacgagga gacctttctc agggcggctc
     2341 gtctggttgg tgagcctgct cgccaggtgt cggcaggatg gacgagactc ttcgtcgaga
     2401 aggtgtccga tcccctcgtc gcagaagaca tctctgtcga gcagcgcatt tccgctgtcg
     2461 tcgggtcgac tgagaggttg accaagcttg ctcctcccct gttgctttgg ctcttcaatc
     2521 gccaccttcg ccacgccatc gaccaggcca acatcgatgg catggaacgc gagttggttg
     2581 cgcacgggct cgtcatcccg ggccccgacc agccgaccgc catcgccttc gtcgacatct
     2641 ccggctacac gagcatgacc gaagctcgcg gggacgagct ggctgcccaa gctgccgaac
     2701 gtctccggca tctggcggcc cttgccaccc actcccattc gggcacggtc gtcaagctcc
     2761 tgggagacgg tgtcatgctt cacttcgaca cggtcgatgc tggtctagcg ggtgtgttcg
     2821 aactcgtgga gagtctcgaa gcatcggatc tacctgcgca cgccggaatt catgccggcc
     2881 ccgtcatcga gcatgacggc gactattacg ggcgaaccgt gaacctggct tcccgggtcg
     2941 ccggtgtggc caccgccggg acggtcctgg cgaccggagg agtggtctcg gcagtcgaga
     3001 ccggtgacta cacgtttcac ccgttccccg aagtcgagct caagggcgtc tctgagccgg
     3061 tctcgttgta taaagcgaca aaggtgggct gacgaagcgg ttggattgcg cggatagtcg
     3121 gtgccctgcg tctgacgtgt aacgaacacg cctatgccgt gttcgttaca catcagttgg
     3181 aggaatgagc gtggccatat gatcggaaca gaatgacggg caccggaaca gcggttccca
     3241 acctcgggaa tgacccatgt gagtcgtgac ttgctcgaga cgatctcacc ctccgagtgg
     3301 tcggtggcat caccggaaca gatcatcgag gcacctgctg atggcgtcta tccgctgctc
     3361 ggtagttccc cggacggtct gtctatcgcc gaagcggaac tgaggttggg ggagtacggg
     3421 ccaaacactc tgagcccgcc ggcgagaaga cccctgatca gtcggttcgt ctccaatttc
     3481 actcacttca tggcgttgtt gctctgggtg ggtggcgccg tggccttcgc cgctgatctg
     3541 cccgagctcg gtgtggcgat ctggatcgtc aacctgatca acggggtctt cagcttctgg
     3601 caggagtaca aggcggagaa ggccaccgaa gccctgctcc gattgcttcc gaccacagcg
     3661 actgtgttga gaggaggtct gcccttggag gtggtcgcca ctgacttggt gcctggggat
     3721 gttgtcatcc tcgaggaagg cgactcgatt tcggcggacg gccggttagt cgagcacgtt
     3781 gcgcttcgag tcgaccagtc gacactcacc ggcgagtcgc acctggtccg caaggcctca
     3841 gatcctgtgg atccaagggg tagcggtcgc ctcgagattc caaatctggt gcacgcaggc
     3901 accacagtgg cctcgggacg gggaaagctg gtggtcaccg ccactggagc caacacccag
     3961 ttgggggcta tcgccgagat gaccggctct ctggcggaga ctccgagccc gctgcagagg
     4021 gagctcaaac ggctgagtgt cgtggtcagc gtcatc
//
LOCUS       DASUTA010000383         2336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1927244, whole genome shotgun sequence.
ACCESSION   DASUTA010000383 DASUTA010000000
VERSION     DASUTA010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1927244"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VFZ80_07625"
     CDS             <1..902
                     /locus_tag="VFZ80_07625"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018396255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6287339.1"
                     /translation="LPGGSEDTVSEGTAAARSTDRVLGVNAWLVFLFLYAPILLLIFF
                     SFNDNDNVAIWTEPSLRWYGEMFRNDQVMAALRNSLLVALVATAVSTVLGTALAISLE
                     RYRYRGRGTLDGLAYLPIIIPDVTMAVMLLIFFFQAFGILDSLTGIRLTTGLHTIMLA
                     HIAFNISFVAVVVRARIAQFDDALEEAAADLYATRWQTFRKVTLPLIAPGVAGGALLA
                     LTLSLDDVVVTQFVSGAGSTTLPVYVFGLVRRGVTPLINAVSVVMLVASMVLVAVSLG
                     LQRSTAGRQRLITEPSAVYERAT"
     gene            973..2067
                     /locus_tag="VFZ80_07630"
     CDS             973..2067
                     /locus_tag="VFZ80_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6287340.1"
                     /translation="MKRAKKLVVALAAFSLFITACGDGGDAGADPTTCELDQVDGDLN
                     FYNWSEYIDPELITAFEEEYGVDVTEDFYESNEAMLAQLQSGAIYDLIVPSDYMVGIM
                     IEEGLLHEVNDAAVPNLANLDPKFNELPYDPGPEYSAAYQFGTTGLGVNTAVVGEDFT
                     PSWALLFDPELTTNFPGGVSVLNDPRETMGAALKYLGYSLNDTDLDHLAEARDLIAAA
                     KDGIATFDSDQYDEALVNQEVAVSHGYSGNMIVAIGEAENPDDFQYVLPEEGATFWID
                     NMAVPTSAESPCTAFTFMNYLLDAENGATLTNWNYYGSPNKASIDAGLIEQEVVDFYA
                     DTFTAEGLEIIEDTGSYEINFTDYLAEAKS"
     gene            complement(2129..>2336)
                     /locus_tag="VFZ80_07635"
     CDS             complement(2129..>2336)
                     /locus_tag="VFZ80_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287341.1"
                     /translation="EPWAPGETVRVAVGNLVEGLQGAADWLIGFGIYALPMLLITLGP
                     LALIGLVVYRRFFRKPHDPAPASS"
BASE COUNT          445 a          733 c          705 g          453 t
ORIGIN      
        1 tacttccggg cgggagcgaa gacactgtga gcgaggggac cgcggccgcc cgatccacgg
       61 accgggtgct gggagtcaat gcctggttgg tcttcttgtt cctctacgca ccgatcctcc
      121 tcctgatctt cttctcgttc aatgacaacg acaacgtggc gatctggacc gagccctcac
      181 tgcgctggta tggcgagatg ttccgtaacg atcaggtgat ggcggctttg cgcaattctc
      241 tgctggtcgc cctggtagcc actgcggtct ccacggtcct ggggacggct ctcgcgatat
      301 cgctcgagcg ctatcgctat cgagggcggg ggaccctcga cgggctggcg tatctcccca
      361 tcatcatccc tgacgtcacc atggcggtga tgctcctgat tttcttcttc caggccttcg
      421 ggatcctcga ctcgttgacc ggaatccgac tcacgaccgg tctgcacacg atcatgctcg
      481 cccatatcgc cttcaacatc tcgttcgtcg ccgtggtggt cagggcgaga atcgcacaat
      541 tcgacgacgc tctcgaggag gctgcggcag acctctacgc cacacggtgg cagacatttc
      601 gcaaggtgac cctgcccctc atcgctcccg gcgtggctgg gggcgctctc ctcgctctga
      661 ccctttccct cgacgacgtc gtggtcacac agttcgtatc cggggccggc tcgaccacgt
      721 tgccggtata cgtattcggt ctggtccggc gtggtgtgac tccactcatc aacgcggtga
      781 gtgtcgtgat gctggtggcc tcgatggtct tggtggcagt ctcgctcggc cttcagaggt
      841 ctactgcggg caggcagaga ctgatcaccg agccctctgc ggtctacgag cgggcgactt
      901 gacgttgcga tttgccctaa ggtctccgac gccggcatgc cggcacccct tgttgcttgg
      961 gaggatgagc atatgaaaag agcgaaaaaa ctcgttgttg ccttggcggc tttctcgctg
     1021 ttcatcaccg cgtgtggtga tggcggcgat gctggcgctg atccgacgac atgtgagctc
     1081 gatcaggtcg atggtgacct gaacttctac aactggtccg agtacatcga ccccgaactg
     1141 atcaccgcat tcgaagagga gtacggggtg gatgtgaccg aggacttcta cgagtccaac
     1201 gaggccatgc ttgcccagct ccagtctggc gccatctacg acctcatcgt cccctccgac
     1261 tacatggtgg ggatcatgat cgaggaagga ctccttcacg aggtcaacga cgcggccgtc
     1321 cccaacctcg caaacctcga cccgaagttc aacgagctgc catatgaccc gggacccgag
     1381 tactcagcgg catatcagtt cgggacgacc gggctcggag tgaataccgc ggtggtcggc
     1441 gaggacttca cgccgagttg ggcactgctg ttcgatcccg agctgacgac gaacttcccg
     1501 ggcggtgtct cggtgctcaa cgacccgagg gagacgatgg gcgcggccct gaagtatctc
     1561 ggttactcac tcaatgacac cgatctggac catctcgccg aggcgcgcga tctgatcgca
     1621 gcagccaagg atgggatcgc cacattcgac agcgatcagt acgacgaggc cctggtgaac
     1681 caggaagtcg cagtgtcaca cggctactcc gggaacatga tcgtggcgat cggcgaggcg
     1741 gagaaccccg acgacttcca atacgtcctg cccgaggaag gcgcgacgtt ctggatcgac
     1801 aacatggcgg ttccgacgtc cgcggagagc ccatgcacgg ccttcacgtt catgaactac
     1861 ctcttggacg cggagaacgg ggcgacgctc accaactgga actactacgg aagccccaac
     1921 aaggcttcga tcgacgccgg tctcatcgag caggaagtcg tcgacttcta cgctgacacc
     1981 ttcaccgccg aaggcctcga gatcatcgag gacaccggga gttacgagat caacttcacc
     2041 gactacctgg ccgaggcgaa gagctagcca gaatctgacc cgtagccagt gaatggcccc
     2101 gggcaatgcc cggggccatt cacaatcgct atgagctcgc cggcgccggg tcgtgtggct
     2161 tgcggaagaa ccgcctgtac acgaccaggc cgatcagggc caacgggccc agagtgatca
     2221 gcagcatcgg aagggcgtag atgccgaagc cgatcagcca gtcggcggcg ccctgtagac
     2281 cctcgacgag gttgcccacc gcgacgcgga cagtctcccc tggagcccac ggctcg
//
LOCUS       DASUTA010000384         2905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_500289, whole genome shotgun sequence.
ACCESSION   DASUTA010000384 DASUTA010000000
VERSION     DASUTA010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500289"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..865)
                     /locus_tag="VFZ80_07640"
     CDS             complement(134..865)
                     /locus_tag="VFZ80_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6287342.1"
                     /translation="MALRSFADGALFAEVYGSGPPTVLALHGWGRRGSDFAKSLEGLS
                     GIAPDLPGFGASPVPREPIGADAYADIVVEILDSFDRPPVLVGHSFGGRVAVCLAAKH
                     PGRVGDLVLTGVPLIRLETGRRPPAVYRLVRWLNSIGLVSDERIEREKKRRGSADYRA
                     ADGVMRDILVKVVNESYEGQLGRIDKRVHLLWGADDTEVPVSVAEAASALLSNSKLEV
                     LPGVGHFVPLQAPGELRHAIDVALA"
     gene            927..2738
                     /locus_tag="VFZ80_07645"
     CDS             927..2738
                     /locus_tag="VFZ80_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6287343.1"
                     /translation="MTNENVSVSVHESMARFRASLLRRASAVAWIVLIIVGYQAVRVT
                     VDDNGTQLFTQPEFFIPMASLAGLLILSTAIRWDRIMRSGAAPWAATAWLLGLIAGIS
                     ALAIIPELQPTAEPILFGITAVSGLLLAWWAHALVTVIIALAIGFISISVGEASQAID
                     VLAQIVVIFVVAAATALVGFEFEKEAVTSFGKEAKLEQQRLDFERLYAVSATLARAES
                     LAEIVPHLIGTICKYLDAQVGVVLLHDGEMQRLEVMSPIWVNGYPLETDPISVEINAP
                     SVVAQTYRAAKPIYVKRVDEDTESHILLKDLGLKQALVAPLKVESMRVGAIVVGDPYE
                     GDFTEEQLEQLGSLAAPAGLVLSQIGRYEAQAEMTRRLEEVAQMKTDFVSTVSHELRS
                     PLTSIIGSLDTVNRPELAPTEPTSQQLLISARRQAGRLQRLIEDLLVVSRIDRGAIPV
                     HPEPVEIKAMFDEVNRVVSIEPAMTVEPSNLAVMADRDHFSRILINLVENAAKYAPDS
                     PVELYAWQKGQRAMVAVVDHGPGIPDHARESIFERFTQLDQSDTRSKGGTGLGLSIVK
                     SLAEVMHGTVRVEATEGGGATFVLDLPAAAVATVPSV"
     gene            complement(2730..>2905)
                     /locus_tag="VFZ80_07650"
     CDS             complement(2730..>2905)
                     /locus_tag="VFZ80_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6287344.1"
                     /translation="EVWSERSPLRWDTSDPDPVARMVCVLLSDWAKMTSGEIVHVDGG
                     FHAVAAGVDPSSD"
BASE COUNT          536 a          968 c          905 g          496 t
ORIGIN      
        1 agcgtcgatc gccaccatcg ccacgcaaag gtgctgacct cgccgggcaa gtagtgctcg
       61 cgctgagcca cgcgcagcca tcgcataccc gccgggatcg aggcgagtgc acagaccgcg
      121 gcgaggagcc agatcatgcc agggccacgt cgatggcgtg tcgcagctcg ccgggcgcct
      181 gcagcgggac gaaatgacct accccgggaa ggacctccag cttggagttc gacagcaacg
      241 ccgacgccgc ctcggccacc gagaccggca cctccgtgtc gtcggcgccc cacaggagat
      301 gaacccgttt gtcgattcgc cccaactggc cctcgtagct ctcgttgacg accttgacga
      361 ggatgtcgcg catcacgccg tccgcggccc gatagtcggc ggaccctcgt ctcttcttct
      421 ctctttcgat tcgctcatcg gagaccagcc caatcgagtt cagccagcgc accagccggt
      481 agacggccgg gggtcttcgc ccggtctcga gccgtatcag gggcacgccg gtcagcacca
      541 gatcgcccac ccgtcctggg tgcttcgccg cgaggcacac agcgacccgg cccccgaagg
      601 aatgacctac gaggaccggg ggcctgtcga aggaatcgag gatctcaacg acgatgtcgg
      661 cataggcatc cgctcctatc ggctctctcg gaactgggga ggctccgaat ccagggaggt
      721 cgggtgcgat gccggacagg ccctcgagtg actttgcgaa gtcactcccc cgtctccccc
      781 agccgtggag ggcgaggacg gtgggtggcc ccgatccata gacctcggca aagagcgcac
      841 cgtcagcaaa tgaacgcaga gccaataaca atcctcgata agaccgggag aaacgccgag
      901 aatacttttg tgcggctccc aatcccatga ccaacgagaa cgtctctgtg agcgttcacg
      961 agtcgatggc ccgattccgg gcgtctctgc ttcgacgcgc ctcggcggtg gcctggatcg
     1021 tgctcatcat cgtcggctac caggcagtac gggtcaccgt tgacgacaac ggaacccagc
     1081 tgttcaccca acccgagttc ttcatcccga tggcttcact cgccggtctg ctcatcctct
     1141 cgacagcgat tcggtgggat cggatcatgc gctcgggcgc ggcgccgtgg gcggcgacgg
     1201 cgtggctgct cgggctgatc gccgggatca gcgcccttgc gatcatcccc gagcttcagc
     1261 cgacggccga gccgatcctg ttcgggataa cagcagtctc gggtcttctc ctcgcctggt
     1321 gggcccatgc gctggtcacc gtcatcatcg ccctggccat cgggttcata tcgatctccg
     1381 tgggagaggc cagccaggcc atcgacgtgt tggcgcagat agtcgtgatc ttcgtggtgg
     1441 ccgcggccac tgccttggtc ggcttcgagt tcgagaagga ggcggtgacc tcgttcggga
     1501 aggaagcgaa gctcgagcaa caacgtctcg acttcgagag gctctatgcg gtgagcgcca
     1561 cactcgcccg ggcagagtcg cttgctgaga tcgtccccca cttgatcggc accatctgca
     1621 agtacctcga cgcacaggta ggagtggtgc tcctccacga cggggagatg cagcgtctcg
     1681 aagtcatgag cccgatctgg gtgaacggct atccactgga gaccgacccg atctctgtcg
     1741 agatcaacgc gcccagcgtc gtcgcccaga cctaccgggc ggccaagccg atctacgtga
     1801 agagggtcga cgaggacacc gagtcgcaca tcttgttgaa ggatctcggt ctgaaacagg
     1861 ccctggtcgc gccgctcaag gtcgaatcga tgcgggtcgg cgccatcgtc gtcggcgatc
     1921 catatgaggg agacttcacc gaggagcaac tcgagcaact cggctccctg gccgcaccgg
     1981 ccgggctggt cttgagccag atcggccgtt acgaggcgca ggcggagatg acgcgccgtc
     2041 tcgaggaggt cgcccagatg aagacagact tcgtgtcgac cgtcagccac gagttgcgca
     2101 gccccttgac atcgatcatc ggctcactcg acaccgtcaa ccggcccgaa ctggccccca
     2161 ccgagccgac ttcccagcag ctgctgatca gcgcccgacg tcaagcaggg cgtctccaga
     2221 gactcatcga ggacctgctg gtcgtttccc ggatcgaccg gggggcgatt ccggtccatc
     2281 ccgagccggt ggagatcaag gcgatgttcg acgaggtcaa ccgggtcgtg tccatcgagc
     2341 ccgcgatgac tgtggagccc tcgaacctgg cggtgatggc cgaccgggat cacttctcgc
     2401 ggatactcat caacctggtc gagaacgcgg cgaagtacgc accggactca cctgtcgagt
     2461 tgtacgcctg gcagaagggt caacgcgcca tggtcgccgt ggtcgaccat gggccgggaa
     2521 tccccgatca cgcccgggag agcatcttcg agcgcttcac ccaactcgat cagtccgaca
     2581 cccgatcgaa gggtggcacc ggcctcggtc tctccatcgt caagagcctg gcggaggtga
     2641 tgcacggcac ggtccgcgtg gaggcgaccg aagggggtgg cgccaccttc gtcctcgacc
     2701 tgccggccgc cgccgtggcg accgtcccgt cagtctgagc tgggatcgac tcctgcggct
     2761 acggcgtgaa agccaccatc tacatgcacg atctcgcccg aggtcatctt cgcccaatcg
     2821 ctgagaagca cacagaccat ccgggccacc gggtccgggt ccgaagtgtc ccatcggagc
     2881 ggagatctct cactccagac ttcat
//
LOCUS       DASUTA010000385         2978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1019633, whole genome shotgun sequence.
ACCESSION   DASUTA010000385 DASUTA010000000
VERSION     DASUTA010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019633"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VFZ80_07655"
     CDS             complement(<1..941)
                     /locus_tag="VFZ80_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6287345.1"
                     /translation="MATDTRKEPKEKPRRSRLQSKLTDERGRLTTDLVSQYLTAIGEY
                     DLLTAEQEVELAQKIEAGEEAAEKLEEGDYKGKREEMELKRLARRGAQAKDDFLTANL
                     RLVVANARRYANTSGIDFLDLIQEGNLGLIRAVEKFDWRKGFKFSTYATWWIRQAITR
                     AIADKSRTVRIPVHLHDTLAAVRAAQASLKAELGRDPRPDEIAEEAGVTVDKVELALS
                     VADTVSLEQPIGEDGAQLGDFIEDEDAADPVQMTEEMDVANSLRQSIDRLPEREGRIL
                     ALRYGFFDGVPRTLEEIGDEFNLTRERIRQLEKLALCR"
     gene            complement(1334..2158)
                     /locus_tag="VFZ80_07660"
     CDS             complement(1334..2158)
                     /locus_tag="VFZ80_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6287346.1"
                     /translation="MTWKLDCGSYTLFVIEDGYYWRDVATYDDRATEHDWRFHPRNEK
                     GQARANFGCCLITNGDRTIMIDAGIGEVTQEGMTAGLMPQAVDALGISPESVETVVYS
                     HMHYDHIGGSSRNGEIVFPEARHVFHTKEWDHWKSVDTEFGKAAQNIMRPLFDADLVD
                     FIDSDTEVIPGVTAVESFGHTPGHLTVSIISDGTRTLIGGDLSNHPFQVEHPHWSLPV
                     DNDKELAAKTRDRVFESIKDTDTTFVAGHYPMPGIGKIVTDDGIRVYQPGSAPKVG"
     gene            complement(2251..2739)
                     /locus_tag="VFZ80_07665"
     CDS             complement(2251..2739)
                     /locus_tag="VFZ80_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6287347.1"
                     /translation="MRQLLLMRHAKSDWSADYSTDHDRPLNDRGVRSARAMGRVLRDN
                     DQVPDLVVTSSATRAKTTAQLAADAGAWESEIVVDSRLYGAGADSVVEMATEFGETKR
                     LMLVGHQPTWSIVVSLLTGESVEMKTATVAVLRFDLSAWSELPGATGEVEKVFQPRDY
                     ID"
     gene            complement(2769..>2978)
                     /locus_tag="VFZ80_07670"
     CDS             complement(2769..>2978)
                     /locus_tag="VFZ80_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX6287348.1"
                     /translation="VQSMPASVSDEGPNLRGVEDKIEQRKAEAMARAELHEATPEGAE
                     AELRKAVNMSEANAKLDELRAELGL"
BASE COUNT          504 a          947 c          920 g          607 t
ORIGIN      
        1 cgacacaacg ccaacttctc cagctgacgg attcgctccc gggtgagatt gaactcgtca
       61 ccgatctcct cgagcgttcg aggcaccccg tcgaagaacc catagcgcag ggcgagaatg
      121 cggccttcgc gctcggggag gcggtcgatc gactgacgga gcgagttggc gacgtccatc
      181 tcctcggtca tctggaccgg gtccgcggcg tcctcgtcct cgatgaagtc gccgagctga
      241 gcgccgtcct cgccgatcgg ctgctcgagt gacaccgtgt ccgccacgga gagcgccagc
      301 tcgaccttgt cgacggtgac acctgcctct tcggcgatct catcgggacg ggggtcacga
      361 cccaactcgg ctttgagcga agcctgagcg gcgcgaaccg ccgccaacgt gtcgtgtagg
      421 tgcaccggga tgcgcaccgt gcgcgatttg tcggcgatgg ctcgagtgat cgcctggcgg
      481 atccaccagg tcgcgtatgt ggagaacttg aatcccttgc gccagtcgaa tttctcgacc
      541 gcacggatga gacccaggtt cccctcttgg atcaggtcga ggaagtcgat gccggaggtg
      601 tttgcgtacc ggcgggcgtt ggccacgacg agacgaaggt tcgctgtgag gaagtcgtcc
      661 ttggcctggg caccccgacg agcgagcctc ttcagctcca tctcctcacg cttgcccttg
      721 tagtcgccct cttccagctt ctccgccgcc tcttcgccgg cctcgatctt ctgggcgagc
      781 tccacctcct gctcggcggt gagcaggtcg tactcgccga tcgcggtgag atactgcgag
      841 acgagatcgg tggtgagccg tccccgctcg tcggtcagct tggattgaag ccgggagcga
      901 cggggtttct cttttggttc tttacgcgtg tcagtggcca ttgattttcc gtgtcctcca
      961 gggcaagtca ggtcgtcggg gaagtcgacc cgagcgttca actatacgca agggtggggg
     1021 gatgtgaacc cccctctttt ccacaagggt aggacgaacg atcggttgca tcggttcccg
     1081 accttcgttg cccactgaag acaacggttc ccgggtggga ggcattcccg agtcttcgct
     1141 gagacaccag cggccccggt cgggccagaa tgcccgagcg cagctgtgtt atgacggatc
     1201 tccgccgagc aaggccacgt gcggagatcc gtcgtgaagg agggccctca tcactgtccc
     1261 ccggctctcc ttcgtccccg gtgcacccca gaagagtcac cagatggcga tgtgatacct
     1321 cgagttgcag cagctaaccc accttcgggg cagatccggg ttgatagacg cggatgccgt
     1381 catcggtgac gatcttgccg atgccgggca tcggatagtg gccggccacg aacgtggtgt
     1441 cggtgtcttt gatcgattcg aagaccctgt cgcgtgtctt ggcggcgagc tccttgtcat
     1501 tgtccacagg caggctccag tggggatgct ccacctggaa cgggtgatta ctcaggtcac
     1561 cgccgatcaa cgtccgggtg ccatcggaga tgatgctcac ggtcaggtgc ccgggagtgt
     1621 gcccgaatga ctcgaccgcg gtcactcccg ggatgacctc ggtgtcgctg tcgatgaagt
     1681 cgacgaggtc cgcgtcgaag agcggtcgca tgatgttctg tgccgccttt ccgaactcgg
     1741 tgtcgacact cttccagtgg tcccattcct tggtgtggaa cacgtggcgt gcctccggga
     1801 agacgatctc cccgtttctg ctgctgcccc cgatgtggtc gtaatgcatg tgggagtaca
     1861 cgacggtctc gacagactcg ggcgagatcc cgagtgcatc taccgcctgg ggcatgagcc
     1921 ccgccgtcat cccctcctgg gtcacctctc cgataccggc gtcgatcatg atggtcctgt
     1981 ctccattggt gatcagacag cacccgaagt ttgcccttgc ctgtcctttc tcgttcctgg
     2041 gatgaaaacg ccagtcgtgc tcggtggcac ggtcgtcata agtggcgacg tcacgccagt
     2101 agtaaccgtc ttcgatgacg aaaagggtgt aggagccgca gtcgagtttc caggtcatgg
     2161 gctcgatcat gccagaaccg aggcgcacca gtgctgggct ctcatccgga aagggcggcg
     2221 atgcgatatg tccaaatcgg atcgggatga ctaatcgatg tagtcgcgag gctggaagac
     2281 cttctccacc tcaccggtgg caccaggcaa ttccgaccaa gcggaaaggt cgaaacggag
     2341 cacggcaacc gttgcggtct tcatctcgac gctctcgccg gtgagtagcg agaccacgat
     2401 ggaccacgtc ggctggtgcc cgaccagcat cagcctcttc gtttctccga actcagtcgc
     2461 catttcaacc acggaatcgg cacctgcgcc gtagagacgt gagtcgacga cgatctccga
     2521 ctcccaggcc ccggcatcag cggccagctg agcggtcgtc ttcgcccgag tcgccgatga
     2581 cgtgaccacc aggtcgggaa cctggtcgtt gtcgcgcaac accctgccca ttgcccgagc
     2641 gctgcgaacc ccgcgatcgt tgagcggacg gtcgtggtca gtggagtagt cggccgacca
     2701 atcagatttg gcgtggcgca tcagaagcag ctgcctcatg ccgccgccgc cggtatccca
     2761 gaaacgggtt acagccccag ctcggcgcgc aattcgtcga gcttggcgtt cgcctccgac
     2821 atgttcactg ccttgcgaag ctctgcctct gcaccctccg gggtcgcctc gtgcagctcg
     2881 gccctcgcca tcgcctcggc tttgcgctgt tcgatcttgt cctccacgcc gcgcaggttg
     2941 ggcccttcgt cggagaccga cgccggcatc gactgcac
//
LOCUS       DASUTA010000386         3193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_243946, whole genome shotgun sequence.
ACCESSION   DASUTA010000386 DASUTA010000000
VERSION     DASUTA010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243946"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..1145
                     /locus_tag="VFZ80_07675"
     CDS             255..1145
                     /locus_tag="VFZ80_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018843269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX6287349.1"
                     /translation="MDIPEVVRKKAEVAGAHEWIRHLPTLIADLEDEWACRVIRPLDG
                     ATEAYVAQVALGGGELAVLKLLIPRDSIAVANEIEVLRLVDGDGCPRLYRADPSRGAI
                     LMELLGDSLESMGLSMAHRHQILCEAARRIWRHVDVDLPTGAEKGEWLIESIVRKWES
                     LDRPLPERVIDHAVDCGRKRIAAHDPTRAVLVHGDVHQWNALATDDGFKLIDPDGLLA
                     EPEYDLGVIMREDPAELMESDPFQRAVRLASLSGLDPNAIWEWGVVERVSTGLILTEI
                     GLQPVGSQMLAAAEYVTLSA"
     gene            complement(1146..1514)
                     /locus_tag="VFZ80_07680"
     CDS             complement(1146..1514)
                     /locus_tag="VFZ80_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX6287350.1"
                     /translation="MTLPRWLAKFNRRFINPKAERGGQWPVLVHVGRNSGVTYRTPIG
                     AIPVDRSYVTFVNYGRRTDWLQNVLAAGEARLETDGESVPVANPRLIPYEVGLEMVGP
                     DGDAPPGWVGVEECLIFDRS"
     gene            complement(1511..2533)
                     /locus_tag="VFZ80_07685"
     CDS             complement(1511..2533)
                     /locus_tag="VFZ80_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018637999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX6287351.1"
                     /translation="MEDTNHHVLSEHRTKTMRAAVQNGFGNARDVIEIKEVPVPDLEP
                     DRVLVEVAAASLNALDHHYLTGTPRIARLDMGLRRPKRTIPGADIAGTITAIGSDVTD
                     WEVGDEVFGEIGGGGFAEFARPRASVLAPKPANVTMAEASTLGVAALTALQGLRDWGG
                     LQPGQTVLINGASGGVGTFAIQIAKALGADHVTGVCSSANVETASALGADRVIDYTKD
                     DFTALNDRFDLFFDNAGSRSLGRSRKMIEDDGVLVMVTGEKGGWIRPVDRVLAGVIRS
                     RFWSQRFVSKTARADRSDLLVLKDMVESGLVKPVMDRTFSLEEAADALAYQSEGHARG
                     KSVVIP"
     gene            2624..>3193
                     /locus_tag="VFZ80_07690"
     CDS             2624..>3193
                     /locus_tag="VFZ80_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020640738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX6287352.1"
                     /translation="MVSAERSSPRTPLSRDRVLAAAVELADRDGIEALTMRGLADRLG
                     VEAMSLYHHVANKEDLLDGVCELVLEEINEAVAGQPLATGPDDWKQVMRLRILTARSV
                     VLRHRWVPAILETRTTMSPATLHYHHGILAIFRAGGFSYDLAHHALHALGSRAIGFTQ
                     ELFDPSPGSAEEEQAMMAFEEMAAQVPLLL"
BASE COUNT          564 a         1006 c         1010 g          613 t
ORIGIN      
        1 ttcatctggg cgtccgtagg acccgatttc gcgaaccgaa taaccggcct tcgggcaccc
       61 aaccccacgg acgcccagat gaaagctccg agatcgcgcg tccctgaagc acacacccca
      121 gccacgcaac gtgagccgac ctgtgacttt aagtggcgtc ctcggggatt gccctcgaat
      181 cctggatcta tcttccaccg ctctcgctgg agcgaacgcg gccgttcggg acatatctcg
      241 atgggaaggg agccatggac atcccagaag tggtgcgcaa gaaggccgaa gtcgccggtg
      301 cccacgaatg gatacggcat ctgccgacgc tgatcgctga tctcgaagac gaatgggcct
      361 gccgtgtgat ccgccccctg gacggggcca ccgaggctta tgtcgcacaa gtcgccctcg
      421 gcggcggtga gcttgccgtg ctgaagttgc ttatcccccg cgattcgata gccgtcgcca
      481 acgagataga ggtactgagg ctcgtggacg gcgacggctg tccccgcttg tatcgggcag
      541 atccgtctcg aggagcgatc ctgatggagc tgctcggtga ttcgctcgag agcatgggat
      601 tgtcgatggc ccatcgccat cagatcttgt gtgaggcggc ccgtcgcatc tggcgacatg
      661 tggatgtcga cctacccacc ggtgccgaga aaggggagtg gctcatcgaa tccatcgtcc
      721 gcaagtggga gtcactcgat cgtccgttgc ccgagcgcgt catcgaccat gccgtcgatt
      781 gcggacgcaa gcgaatcgcg gcccatgacc cgacgcgagc cgttctggtc cacggtgatg
      841 tccaccaatg gaatgcgcta gccacagatg acgggttcaa gctgatcgac cccgatggtc
      901 tcctcgccga gccggagtac gacctggggg tgatcatgcg ggaggacccg gccgagctga
      961 tggagtccga tccgttccaa cgggcggtcc gtctggcctc tctcagcgga ctcgacccca
     1021 acgcgatttg ggagtggggg gtggtggaga gagtctccac cggcctcatc ctcaccgaga
     1081 tcggtcttca gccggtcggc agccaaatgc tggctgcggc tgagtatgtc acgctctcgg
     1141 cctgatcagc ttcgatcgaa gatcaggcac tcctcgaccc cgacccatcc cggcggtgcg
     1201 tcgccgtccg ggcccaccat ctccaatccc acctcgtacg ggatcagcct ggggttggcg
     1261 accggcaccg actcgccgtc ggtctccagc ctggcctcgc cggctgccaa cacattctgc
     1321 agccagtcgg tcctccgtcc gtagttgacg aaggtgacgt acgaccggtc cacagggatg
     1381 gccccgatcg gcgtgcggta tgtgacaccc gagttcctgc cgacgtgcac gaggaccggc
     1441 cattggcctc ctcgctctgc ctttgggttg atgaagcgtc tgttgaactt ggccaaccat
     1501 cgaggcaggg tcatgggatc accacggact tgccacgggc gtgcccttcg ctctggtagg
     1561 ccagtgcatc ggcggcctcc tccagactga acgtgcgatc catgacaggt ttgacgagtc
     1621 cggactcaac catgtccttc aggacgagga ggtcggatcg gtcggctctt gccgtcttgc
     1681 tcacgaaccg ctgactccag aatcgcgacc tgatcacgcc agcgagcacg cgatcgacgg
     1741 ggcgaatcca gccacccttc tctccggtga ccatgacgag aacaccgtcg tcttcgatca
     1801 tcttccgcga acgaccgaga gaccggctgc cggcgttgtc gaagaagagg tcgaagcggt
     1861 cgttgagggc ggtgaagtcg tccttggtgt aatcgatgac ccggtcggcc cccaaggcag
     1921 aggcggtctc gacattggcg ctgctgcaga ctccggtcac atggtcggcg cccagcgcct
     1981 tggcgatctg gatggcgaag gtccccacac ctcccgaagc gccgttgatg agcacggtct
     2041 ggcccggttg aagaccgccc caatcccgga gaccctgcag ggccgtcagg gccgccaccc
     2101 cgagtgtgga tgcttcggcc atggtcacgt tcgccggctt gggtgcgagc acggaggctc
     2161 ggggtcttgc gaactcagcg aatccaccgc caccgatctc gccgaacacc tcgtctccga
     2221 cttcccagtc ggtgacatcg gagccgatgg ctgtgatggt gccggcaatg tctgcgcccg
     2281 ggatcgttct ctttggtcga cgcaggccca tgtccagtct ggcgattcgt ggcgtgcccg
     2341 tcaggtagtg gtggtcgagt gcgttgaggg aggccgcggc gacttcgacc aggacccgat
     2401 cgggctccag gtcgggcacg ggtacctctt tgatctcgat cacgtcccgg gcattcccga
     2461 agccattctg aaccgctgcc ctcatggttt tggttcgatg ttctgacagg acatggtgat
     2521 tcgtgtcttc cattcgcgct ccattgtcgt acactgtaag gcaacttacc catacggcgt
     2581 aaggttgtca atcgacttac gacgtacgac ttagagttga gtcatggtca gcgcagagag
     2641 atcaagccct cgcaccccgt tgagccgcga tcgggtgctg gctgcggcgg ttgaattggc
     2701 cgaccgcgat ggcatcgagg cgttgaccat gcgcgggctg gccgaccgac tcggggttga
     2761 ggcgatgtcg ctctatcacc acgtcgccaa caaggaagat ctcctcgacg gtgtctgtga
     2821 attggtcctc gaggagatca acgaggcggt tgcgggccag ccgttggcca cgggccccga
     2881 tgactggaaa caggtcatgc gactgaggat cctgacggcg cgttcggtcg tgctcagaca
     2941 cagatgggtg ccggccattc tcgagacgcg gaccacgatg agcccggcga cgctgcacta
     3001 ccaccacggg atactggcca tcttccgggc cgggggcttc agctacgacc tggcgcatca
     3061 tgcgctccac gcactgggga gtcgggccat tgggttcacc caggagctgt tcgatccgtc
     3121 tccgggtagc gccgaggagg aacaggcgat gatggccttc gaggagatgg ccgctcaggt
     3181 ccccttactg ctc
//
LOCUS       DASUTA010000387         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1668013, whole genome shotgun sequence.
ACCESSION   DASUTA010000387 DASUTA010000000
VERSION     DASUTA010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668013"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VFZ80_07695"
     CDS             complement(<1..504)
                     /locus_tag="VFZ80_07695"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6287353.1"
                     /translation="MDLLAKGIVTGVLIGGLYALMSLGVSLSWGALKIINLAHFSFIL
                     FSAYLVYQLTITFEVDPLLTMVVVLPLFFVVGALMQIFFHKAKVDEFKSLIVTFGIFI
                     ILQSLMQTIWSADFRRIGSELNTYASGSLFIGEIALPIPSLMAFIGAVLIAIVMSLLL
                     SRSYFGKA"
     gene            complement(594..1931)
                     /locus_tag="VFZ80_07700"
     CDS             complement(594..1931)
                     /locus_tag="VFZ80_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6287354.1"
                     /translation="MRRQQSWRYFAIATVLMLVVAACGDGGTSDTSAPEGTDAPGTTG
                     ATETTEASGTTEAGGEASGEPITIGGSLALTGFLAPTAAIHQAVGEVFVERLNEQGGL
                     LGRPVEWELLDDESAPDTAAALYERLITEDEVDLVIGPYGTGNITAAMAVAERYGYVF
                     PHHTASLTYAYNYACHFSSWSTGLHPNITNPNLVYDAIESSGNTPETIGFVINEFPGT
                     MFIAYGQEGTDEGGAVSEAEKRGYDVVLDITFPTAITDWAPIAAQVADADPDFLYIGG
                     LGVNASQLITAMQAIDYTPKGMFTQWPAPGPLLETEGADGIMSVTIFEEHPPFTDDPE
                     AVEVAALINEALADAGVPYTKVETQAAASWTAWEYLQQAAEGAGSLDQQAMCDWLLEN
                     GADTLFHGEIEFYPEEQNYYVDLSKVKQIQDGDWYVVYPEEFAAPDRSVNYNP"
     gene            2146..>2554
                     /locus_tag="VFZ80_07705"
     CDS             2146..>2554
                     /locus_tag="VFZ80_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX6287355.1"
                     /translation="MSDTPADRPSPESPESQIESHPEREGISRRRFITGAAAAGAAGV
                     LGVPSIASAATPSTFLRVNGPDPETDLIFVNGKIHTMNDKGTIARTATVRDGRFVAVG
                     RGARARGPRSSVINLRGRTVVPGIIDNHNHIVLM"
BASE COUNT          486 a          792 c          816 g          460 t
ORIGIN      
        1 tgccttgccg aagtagctgc ggctgaggag cagggacatg acgatggcga tcagcaccgc
       61 cccgatgaag gccatgagcg agggtatggg cagagcgatt tcgccgatga acagcgaccc
      121 gctcgcatat gtgttcagct cggagccgat ccgtcggaag tcggcgctcc agatggtctg
      181 catcaggctc tggaggatga tgaagatccc gaacgtcacg atgagcgact tgaactcgtc
      241 gaccttggcc ttgtgaaaga agatctgcat caatgccccc acgacgaaga agagtgggag
      301 caccaccacc atcgtcagta gtgggtccac ctcgaacgtg atggtgagtt ggtagacgag
      361 ataggcgctg aagaggatga aggagaagtg ggcgagattg atgatcttca gcgcacccca
      421 agacagggac actccaaggc tcatcagcgc atacagaccg ccgatcagga cacccgtgac
      481 gattcccttg gcgagtaggt ccacgctctc ctcttacctg gtttcgggga ctcggaccct
      541 acccgggccg gccgcaggtc agcgaccggc cctgagcacg gttctacttg gtatcagggg
      601 ttgtagttga ccgaccggtc gggagcggcg aactcctcgg ggtacaccac gtaccagtcc
      661 ccatcctgga tctgcttcac cttggacagg tcgacgtagt agttctgctc ttccggatag
      721 aactcgatct cgccgtggaa cagggtgtcg gcaccattct ccaggagcca gtcgcacatc
      781 gcctgctggt cgaggctgcc tgcaccttcg gctgcctgct gcaggtactc ccaagcggtc
      841 cacgaagcgg cggcctgtgt ctccaccttg gtgtatggga caccggcatc ggccagggcc
      901 tcgttgatca acgcggccac ctcgacggcc tcggggtcgt cggtgaacgg tggatgctcc
      961 tcgaagatcg tcaccgacat gatcccgtcg gcaccctcgg tctcgagcag aggaccgggg
     1021 gcaggccact gggtgaacat ccccttcggg gtgtagtcga tggcctgcat cgcggtgatc
     1081 agctgagacg cgttgacgcc caggcccccg atatagagga agtcggggtc ggcatcggcg
     1141 acctgggcgg cgatcggcgc ccagtcggtg atggcagtcg ggaacgtgat gtcgaggacc
     1201 acgtcgtagc cacgcttctc ggcctcggaa acggcaccgc cctcgtcggt cccctcctgg
     1261 ccgtatgcga tgaacatcgt gccggggaac tcgttgatga cgaacccgat cgtctcgggc
     1321 gtgttacccg agctctcgat ggcgtcgtag acgaggttgg ggttggtgat gttgggatgg
     1381 agaccggtcg accaagagct gaagtggcag gcgtagttgt acgcgtaggt gaggctggcg
     1441 gtgtgatgcg ggaacacgta cccgtaccgt tcggcgacgg ccatcgcagc tgtgatgttg
     1501 cccgtgccgt agggcccgat caccaggtcg acctcgtcct cggttatcag acgctcgtac
     1561 agggcggcgg cggtgtccgg cgcagactcg tcgtccagca actcccattc cacgggacga
     1621 ccgagcaggc caccctgctc gttcagacgc tcgacgaaga cctcacccac tgcctggtgg
     1681 atcgccgcgg taggagcaag gaatccggtc agggcaagag agccaccgat ggtgatcggc
     1741 tccccgctgg cctcaccgcc cgcttcggtg gtaccgctcg cttcggtcgt ctccgtcgcc
     1801 ccggtcgttc ccggggcatc ggtgccttct ggtgcggatg tgtcggaggt gcctccgtca
     1861 ccacaggcgg ccacgaccaa catcagaacg gtcgcgatcg cgaagtatct ccagctctgc
     1921 tgacgtctca tcctcaatcc tcctcacgag agcttcggct ttcgccgttc agcgacacta
     1981 cacaagccca acaggtcgtg tagtgatact gatgaactca tttccgaacc ggcaagagcg
     2041 agatgactgt catgcactgc acagtgacac tgcacggatc cagtcgattc gtataggttc
     2101 acttcatcgc cggaggaccg cccggcgccg agtgaggaga aggtcatgtc agacacaccg
     2161 gccgacaggc ccagcccgga gtcacccgag agtcagatcg agagccaccc ggagcgtgaa
     2221 ggaatctccc gtcgccgctt catcaccggg gccgcggccg ccggcgccgc aggcgtcctc
     2281 ggtgtcccgt cgatcgcctc ggcggcgaca ccctcgacgt tcctccgggt gaacggtccc
     2341 gatcccgaga cggatctcat cttcgtcaac gggaagatcc acaccatgaa cgacaagggg
     2401 acaatcgcca ggacggccac cgttcgcgac gggcgcttcg tcgcagtggg caggggagca
     2461 cgggctcggg gaccgaggtc ctcagtgatc aacctgaggg gacggaccgt ggtcccgggc
     2521 atcatcgaca accacaacca catcgtgttg atgg
//
LOCUS       DASUTA010000388         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1668402, whole genome shotgun sequence.
ACCESSION   DASUTA010000388 DASUTA010000000
VERSION     DASUTA010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668402"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFZ80_07710"
     CDS             <1..288
                     /locus_tag="VFZ80_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287356.1"
                     /translation="KSEIELALLEYDLDERNERRIRLGAKAAAELESQERENAEKITH
                     TVPAELIGIGVRIEDDILITQDGHENMTAGTPKELDEVEAMCAESSLLPTS"
     gene            complement(372..1040)
                     /locus_tag="VFZ80_07715"
     CDS             complement(372..1040)
                     /locus_tag="VFZ80_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX6287357.1"
                     /translation="MDRITLGRMQNPIRGFLHGSAALAALVGTIYLVARTWGDVAALV
                     GSLIFGGALLVMYTVSSLYHSVPWSEIWKGRLQRVDHSMIYLVVAGTFTPIAIGGLEG
                     LAIWWCLSLVWAIALVGISLKFVLPEVSTKLSVTLQLVMGWTVILWLPQISTSLGTGA
                     IAFIAVGGIFYTSGTVIFMTKRPRLFPRSFSYHELFHVFVVLASVFHFMAVALYAVPA
                     ATAL"
     gene            1152..1919
                     /gene="uppS"
                     /locus_tag="VFZ80_07720"
     CDS             1152..1919
                     /gene="uppS"
                     /locus_tag="VFZ80_07720"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007319815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX6287358.1"
                     /translation="MALGLLYKIYERNLLAGLDPDRLPNHIGVVLDGHRRHARAEGLP
                     SYSDAYRVGMARFEDFLGWSNELRIPAVTAWVLSLENLSRPPEELAPYFEVLIDLFER
                     LPEKCAEYDMALRFIGSLDQLPVDLVEAAKKAEARNQGGSRRLNVAMGYGGRQEIVDA
                     TKDLVTKLAAEGVPADEITDHIDADGLARHMYSADLPDVDLIIRTSGESRLSGFLLWQ
                     SAYSEFVFVDVNWPAFRRVDFLRALRDYAGRTRRFGQ"
BASE COUNT          435 a          673 c          685 g          358 t
ORIGIN      
        1 aagagcgaga tcgagctcgc cctcctcgaa tacgacctcg acgagcgtaa cgagcgccgc
       61 atccgccttg gggcgaaggc ggccgccgag ctcgagtccc aggaacggga gaacgcggag
      121 aagatcacac acacggtgcc cgctgagctc atcggcatcg gcgtccgtat cgaggacgac
      181 atcctgataa cccaagacgg gcacgagaac atgacggcgg gaacgcccaa ggagctcgat
      241 gaggtcgagg cgatgtgcgc agagagctcg ctgcttccga cttcctgacc gcgacccgct
      301 ctggccgccg ggggcacggc gcttggggat atcgcgaccc tgtgcactga gacggccttg
      361 gaaccgtgct cctagagggc cgttgccgca ggaacggcgt agagagccac cgccatgaag
      421 tggaacacgc tggccagcac gacgaagacg tggaatagct cgtgatacga gaagctgcga
      481 ggaaacagac gtgggcgttt ggtcatgaag atgaccgtgc cggaggtgta gaagattccc
      541 ccgacagcga tgaaggcgat cgctcccgtt cctagcgaag tcgatatctg gggcagccag
      601 aggatgacgg tccaacccat caccagctgg agagtcaccg agagcttcgt agacacctcg
      661 ggcaggacga acttcagtga gatcccgacc agggcgatcg cccacaccag gctcagacac
      721 caccaaatcg cgagaccctc cagacctccg attgcgatcg gcgtgaaggt gccggcgacc
      781 accaggtaga tcatggagtg gtcgacccgc tgcagacgcc ccttccatat ctcgctccac
      841 ggcacggagt gatagaggga cgacacggtg tacatgacga gcagggcgcc gccgaagatc
      901 agcgagccga cgagggcggc cacatctccc caggtcctgg cgaccaggta gatcgtcccg
      961 accaaggcgg ccagagctgc ggagccgtgc agaaagccac gtatcgggtt ctgcatgcga
     1021 cccagcgtga tgcggtccat catcgacaga ctaagcccgc cgggtgacaa ggccatatgg
     1081 cggtccgttg cgatctgggc ctttggcctc ttaccgaagt ggcagggcca tcggtaccat
     1141 aaggcttcgg tatggccttg ggtcttctct acaaaatcta cgagcggaac ctcctcgccg
     1201 gcctcgaccc cgatcgactt cccaatcaca tcggagtcgt gctcgatggt caccgcagac
     1261 acgcccgcgc cgagggtctg cccagttaca gcgatgccta tcgggtgggg atggcgcggt
     1321 tcgaggattt cctgggatgg tcgaacgagc tgaggatccc cgccgtgacc gcctgggtcc
     1381 tctccctcga gaacctctcc cgaccccctg aagagctcgc cccttacttc gaggtgctga
     1441 tcgacctctt cgagaggctc ccggagaaat gcgccgaata cgacatggca ctccgattca
     1501 tcggcagcct ggatcagctg cccgtcgacc tcgtcgaggc ggcgaagaag gccgaggcga
     1561 ggaatcaggg cggaagtcgc cggctcaacg tcgccatggg gtacggaggt cgtcaggaga
     1621 tcgtcgacgc caccaaggac ctggtgacca aactggcggc cgagggtgtg cccgctgatg
     1681 agatcaccga ccatatcgac gccgacggcc tggctcgaca catgtactcg gccgatctgc
     1741 ccgatgtcga cctgataatc cgcacttcgg gggagtcccg tctctccggt ttcttgctct
     1801 ggcagtccgc ttactcggag ttcgtcttcg tcgacgtcaa ctggcccgcc ttccgacggg
     1861 tcgactttct ccgcgccttg cgtgactacg ccggtcgcac ccgtcgcttc gggcagtgat
     1921 gactgagggg gacgaacccc ctcaccgatt gggcgtaccc ggggttcctg gggtcatatg
     1981 tggcccgaat ccgctcatct acgaggtgag ccgggtctca ggcggtcacc atgcctgggt
     2041 ggagatggtt gacatcaccc aattgacgca gcgagaaggc ccagccggtg ctcactcgaa
     2101 aggtggtgag ccgggacaca ccggtgtggg gctgacggaa acggtcccat t
//
LOCUS       DASUTA010000389         3626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_375151, whole genome shotgun sequence.
ACCESSION   DASUTA010000389 DASUTA010000000
VERSION     DASUTA010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375151"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..127
                     /locus_tag="VFZ80_07725"
     CDS             <1..127
                     /locus_tag="VFZ80_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX6287359.1"
                     /translation="LEEVGREFRLTRERIRQIESEALSKCRHPSRGRKLISYLDY"
     gene            complement(444..1466)
                     /locus_tag="VFZ80_07730"
     CDS             complement(444..1466)
                     /locus_tag="VFZ80_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX6287360.1"
                     /translation="MRTRFTATVAVAGMVVMLAMVTPAAAGPAPKSKTISFCSPGAYT
                     VTEGEGDSVTINVCRSVKQKTASVTVDVYVTGTDAEVNEDFTWSGPKVVSFARNRTTA
                     SFTVPILIDGDMGAEPNEMIVFELSDPSTGYALVSGPTVRAFVTIADMQAPGTPTLEA
                     WTSADTTISLGWTEVNPAGSTYVVSRSTDGYNWGELTSGTDFYSTDDSGLDLATTYYY
                     EVYAVSLDGAMSLSSYASATTAFPVPPAPLITGTDNLYAATIYWAIDYSYPVEFEVFR
                     SDSGPNASLESFYSVADQIYGSEWTDKSTTAGSVYYYRVRAVIPGDSGVQSELSEEVE
                     VAVIGS"
     gene            3012..3266
                     /locus_tag="VFZ80_07735"
     CDS             3012..3266
                     /locus_tag="VFZ80_07735"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX6287361.1"
                     /translation="MRFLYFYLMKDAPDRVRAVAPDHSAYWKGLGLRGYLGGPFEDRS
                     GGLITFEVGSADEAERLVADDPFVQEDLLERRWVKAWLID"
     gene            3373..>3626
                     /locus_tag="VFZ80_07740"
     CDS             3373..>3626
                     /locus_tag="VFZ80_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6287362.1"
                     /translation="MRRAWERIDSCPTQRLETRIGTVEYVDQGEGVPLLVVHGVLGCH
                     VDTVDGWWANLPGAGFRVIGPSRFGYFGSTLPPGASPADQA"
BASE COUNT          625 a         1154 c         1212 g          635 t
ORIGIN      
        1 gttggaagag gtgggccgcg agtttcgcct cacccgtgag cggatccggc agatcgagtc
       61 ggaagcgctc tcgaagtgtc ggcatccatc gcgggggcgg aagctcatct cctacctcga
      121 ctactgatga cggccttggg ccgcaccgag ctgagggcgg cttgggacgt acccaggact
      181 cgtcaggtgt ccaccagctg gccatcctcc cgcgaaggta gtcacagcgt gatacagggc
      241 acccggtgac agagggtgtc agggattgcc tccacacact ctgaactgca aagttgctgg
      301 tgaatagcca tatggagctt cccttacaag gaagaggtcg ggggatcgac accctcagcg
      361 cccacctcag tcgcacctac aagggacaag gacctcggcg taggccgagg tccttggtca
      421 tcacctcgcg ggatacgtag cggtcaagag cctatgaccg ccacctcaac ttcttctgac
      481 agctcgctct gcactcccga atcgccgggg atcacggcgc gaaccctgta gtagtagaca
      541 gagcccgcgg tcgtggactt gtccgtccac tcactgccgt agatctggtc ggccaccgag
      601 tagaaggact caagcgatgc attcgggccg ctatccgaac ggaacacctc gaactccacc
      661 gggtaggagt aatcgatcgc ccagtagatc gtggccgcgt agaggttgtc tgtgccggtg
      721 atgagcggcg ccggcggaac cgggaacgcg gtcgtcgccg aggcatagga agacaacgac
      781 atcgcaccgt ccagactcac ggcgtacacc tcgtagtagt aggtggtcgc cagatcgaga
      841 ccggagtcat ccgtcgagta gaagtcagtc ccgctcgtga gttcccccca gttgtaccca
      901 tccgtcgagc gcgacacgac ataggtactt ccggccgggt tgacctccgt ccagcccagc
      961 gagatcgtgg tgtcagcgct tgtccacgcc tcgagcgtcg gggttccagg agcctgcata
     1021 tcagcgatcg tcacgaacgc tcgcacggtc ggtccagaga caagggcgta tccggtgctc
     1081 gggtcgctca gctcgaagac gatcatctcg ttcggctccg cccccatgtc gccgtcgatc
     1141 aggatcggga ccgtgaacga cgcggtcgtc ctgttcctcg cgaaggagac gaccttcggc
     1201 ccggaccagg tgaagtcctc attgacctct gcgtccgtcc cggtcacgta cacgtcaacg
     1261 gtcacgctcg cggtcttctg cttgaccgag cggcagacgt tgatcgtcac ggagtcgccc
     1321 tcaccctcgg tcacggtgta cgcaccaggg gagcagaacg agatcgtctt cgactttgga
     1381 gctggcccag ccgcggccgg cgtcaccatc gccagcatca cgaccatgcc cgcgacggcg
     1441 acggtcgccg tgaatcgggt cctcatagct gatcactccc tccggtcggg catgctcccg
     1501 acacatcgat gccatagaag gacgagggaa tcgcagggct tggggggaca ggttgtgggt
     1561 cggggcctca tggcctcatc tggagaaccc ctcggtcacc cgcgggcgct ctgctgcggt
     1621 cgtccccccg actctgtagg ccggctgcgc cgatggcaca ccccaagtgg cccccttcat
     1681 gtcgggggcg gccttagggc gtgcccgccg tacgcaagat cgccacgaag tcctttcttc
     1741 gcggcgagcg ctccgcgccc agagtctccc acggcgggtt tgacccacgg ggcccccatt
     1801 tccgtcaagc aggccaaggc cgaaatgggc agatatcgtg gaagttgggt atctgccgcc
     1861 gccagggcgc tcggaaggac ttcaccaagc agtccgtcga ccaccggcag tgtccgcaga
     1921 tcgtggtcca cgccaacatc gcgatctgga acgagacgaa cgcagtgctg ggaacgagtg
     1981 ctgaggaact ccgtctgcac gagcgacatc tgagtcgtgc gggagattgg aacgagggcc
     2041 ggcgaatcag gccggcggtc tcgctcacct cgaatcagcg cagacagcgc cgccgctgct
     2101 gggtgactcc aagcccgacg gcgagggcta atcgttcgaa gcccgtgagc tgccctgcaa
     2161 gagagaggcg ctgccagtct cggcgtcgga tcgccgcagg cgtccaggtg cgtcatcgac
     2221 cttcgccgaa cacctgcatg ttcgagccct ccaacgccca cggatcccag ctcaaaggac
     2281 agccctgtcg tctcgcctga gttggctctt cgtcgttcga atcgcccggg ataggctcgt
     2341 gcggttggac cggcgacccc gaggagaaga tgacaggttc ggcgacgggg agagggccat
     2401 cgaacaccgg cgttgccatg tactggctgc ctcttggcgt gcgcggccgg tgggtgcgct
     2461 tggggggaag gatgtacgag gccgtcgcag ccgcgctcgc tcgccgagag cgactcgaca
     2521 tctaccactc ggtcctggaa gttcacgcga gcgacggacg cttcgtgatc gagatgggcc
     2581 ccgccctcga cgagcatggg acgcggcgcg gggtcgtcgc gcacggcccc gtcgccttcg
     2641 gttggggcat ccgtcctgcg catcctccgc tacgaggtgc gctgctggcc gaagggagtc
     2701 acccgcgtac gagcatgctg tcgagagtcc gttgcggctg acgcgcgacc cagccgttgc
     2761 ggcgcggatc ctcgaactcg tgcacgatgt cccgacaccc gtgtgggggc gggacgactt
     2821 gatggcaggg gagatatgga cgtgcaaact ccttgacctc gtggctcctc ggtcgagccg
     2881 gtctcgctgg gtcgatccga ccgcccatcg gtggacgtgc gcctggctgg gatgcgggac
     2941 tcacggtcgc ccatcgagga gtggccgcct ccatcgatcg cgacggaggt tgacagcatg
     3001 ggaggtctcg gatgcgcttc ctgtacttct acctgatgaa ggacgcgccc gatcgggtcc
     3061 gagcggtcgc tcccgaccat tcggcgtact ggaaaggtct cgggctgcgc ggatacctcg
     3121 gcggaccctt cgaggacagg tcgggtgggt tgatcacgtt cgaggtcggc tcggccgacg
     3181 aggcggaacg actggtggcg gatgatccgt tcgtccagga ggatcttctg gagcgccggt
     3241 gggtaaaggc ttggctgatc gactgaggcg ttccgggacg ccttcgagat agagagggct
     3301 caccgccctt ggtgcctcct cgaggaggta ccgaggtcgc tgggagggat catgtccgcc
     3361 tatcgggagg acatgcgccg agcgtgggag cggatcgact cgtgcccgac gcagaggctg
     3421 gagacccgca tcggcacggt cgagtacgtg gatcagggcg agggagttcc tctcctcgtt
     3481 gtccacgggg tcctcggctg ccacgtcgac acggtggacg gctggtgggc gaacctaccc
     3541 ggggcggggt tccgcgtcat cggtccgtcg cgcttcgggt acttcggctc gaccctcccg
     3601 cccggtgcga gcccggccga ccaggc
//
LOCUS       DASUTA010000390         2133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_762189, whole genome shotgun sequence.
ACCESSION   DASUTA010000390 DASUTA010000000
VERSION     DASUTA010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762189"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VFZ80_07745"
     CDS             <1..390
                     /locus_tag="VFZ80_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HEX6287363.1"
                     /translation="SLTMVLAHNALTRHDQNGARTWLFATAALGAVFLGGQVFEFTEF
                     IVEYDMTLSTNPAASAFYVLTGFHGTHVAIGVIMLLSLWGISRRQGGLSERHGLNLEL
                     VGLYWHFVDIIWIVIFTVVYLLSVPVT"
     gene            422..733
                     /locus_tag="VFZ80_07750"
     CDS             422..733
                     /locus_tag="VFZ80_07750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX6287364.1"
                     /translation="MTSDQHDNHPSPAQYWKIAAFLALLTAIEVSLFYIDHALGLGGF
                     NAIMLLGLSAVKFLVVVGWFMHVRYEKPMISRFFTAGFILACSLYLVVLASLGVVAIR
                     G"
     gene            complement(800..>2133)
                     /locus_tag="VFZ80_07755"
     CDS             complement(800..>2133)
                     /locus_tag="VFZ80_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287365.1"
                     /translation="PDIESWVTAGNELTDHFINYGYGLGEPFQIFASRSNEILVENGI
                     EEMTVTLDVWANQDCEWRIEVHSPIASPNPCDIHEVFTDRDLEVCLGPFPPIAGHHAI
                     WTGSEVMVFGGTSGALDWKRPPDGFLFDPSTREVSPIPPAPVAEGWSVHEMLWAGDRA
                     LVIGQSLFTGESTDFRPSIVAFDPVTGEWSQVAVFPDEHQVVGAVALAGDRLVFIGGD
                     QNGPSDQVWEYSLADDEWRHLEGAPIPTVEEARAVWTGTEVIVVGGYSGVDETLYMAY
                     DPEDGGWRDLSEPGYQYIEYHHLFWTGEKVIVAPLHVYTEDLGVHNSPTLLVYDPVTD
                     TWTQTPENPARPPIRGAVSWTGTELLSWGGLSGTWHPISEGSAYDPGSDTWRLLSDSP
                     LSGRADHTGTWTGDRWIVIGGSEHAGSPGAPSLSDGAIYDPTSDAWEYLGG"
BASE COUNT          363 a          713 c          636 g          421 t
ORIGIN      
        1 tcgctgacca tggtgctcgc ccacaacgcc ctgacccggc acgaccagaa cggcgcccgt
       61 acatggctct tcgccaccgc cgccctggga gcggtcttcc tcggtggaca ggtgttcgag
      121 ttcaccgagt tcatcgtcga atacgacatg actctctcca cgaaccctgc tgcctctgcg
      181 ttctacgttc tcaccggctt tcacggcact cacgtcgcga tcggggtgat catgcttctc
      241 tcgctctggg gcatctcccg tcgtcagggt ggcctgagtg agaggcacgg gctcaatctc
      301 gagctggtcg gtctctactg gcacttcgtg gacataatct ggatcgtcat cttcaccgtc
      361 gtctatctgc tttcggtgcc agtgacatga acatccatca cccctgccac caaccgatca
      421 gatgacctca gaccagcacg acaaccaccc cagtccggcc cagtactgga agatcgccgc
      481 gttcctcgcc ctgctcaccg ccatcgaggt ctcgctcttc tacatcgacc acgctctggg
      541 cctcggcggc ttcaacgcca tcatgctgct cgggctgtct gcggtgaagt tcctggtcgt
      601 cgtgggctgg ttcatgcacg tccgctatga gaagccgatg atcagccgct tcttcactgc
      661 cggattcatc ctggcctgct ctctctatct ggtcgttctc gcctccctcg gcgtggtggc
      721 gatcaggggc tgaaccgctg aatcagcgaa cccggggttc ctcgggccgt atatggcgcg
      781 aatccgctca tgttcgcggt cagcctccca gatactccca ggcgtccgac gtcgggtcgt
      841 agatggcgcc gtcgctcagc gagggcgctc ccggggagcc ggcgtgctcc gacccgccga
      901 tcacgatcca tcggtcgccg gtccaggtcc cggtgtgatc cgcccgaccg ctgagtggtg
      961 aatcggagag gagtcgccag gtgtcggagc ccgggtcata tgccgaaccc tccgagatcg
     1021 gatgccacgt gccagagagc ccgccccagg acagaagctc ggttccggtc cagctgactg
     1081 caccccggat cggcggccgc gccgggttct caggggtctg cgtccacgta tcggtgacag
     1141 ggtcgtacac gagcagcgtc ggcgaattgt gcactccgag atcctcggtg tagacgtgga
     1201 ggggggccac gatcactttc tctccggtcc agaagagatg gtgatactcg atgtactgat
     1261 agccaggctc ggacaagtcg cgccaaccgc cgtcctcggg gtcgtaggcc atgtaaagcg
     1321 tctcgtcgac accggagtac cccccgacga cgatcacctc agttccggtc cagacggcgc
     1381 gtgcttcctc cacagtcggg attggggcac cttcgagatg gcgccactcg tcgtcggcga
     1441 gggagtattc ccagacctga tcgctgggcc cgttctgatc tcctccgatg aataccaatc
     1501 gatcgcccgc gagggccacg gcacccacca cctggtgctc atcggggaac accgcgacct
     1561 gagaccactc gcccgtcacc ggatcgaagg cgacgatcga cggccgaaag tcggtgctct
     1621 cgccggtgaa cagactctgg ccgatcacca gggcacgatc acccgcccac agcatttcgt
     1681 ggacactcca tccctccgct accggtgccg gcggaatcgg gctcacctcc cgtgtggatg
     1741 ggtcgaagag aaagccgtcc ggtggtcgct tccaatccag ggcgcccgat gtcccgccaa
     1801 agaccatgac ttcgctcccg gtccaaatgg cgtggtggcc ggcgatcggc gggaacgggc
     1861 cgagacacac ttcgaggtcg cggtcggtga acacctcgtg gatgtcacat ggattggggc
     1921 tggcgatggg ggagtgaacc tcgatgcgcc actcgcagtc ctggttggcc cagacatcga
     1981 gggtcacggt catctcctcg atgccgtttt ccaccaggat ctcgttgctc ctcgatgcga
     2041 agatctggaa gggctcgccg aggccatatc cgtagttgat gaagtgatcg gtgagctcgt
     2101 tccccgcagt cacccacgac tcgatgtcgg gat
//
LOCUS       DASUTA010000391         2748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_115411, whole genome shotgun sequence.
ACCESSION   DASUTA010000391 DASUTA010000000
VERSION     DASUTA010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115411"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2748)
                     /gene="nifJ"
                     /locus_tag="VFZ80_07760"
     CDS             complement(<1..>2748)
                     /gene="nifJ"
                     /locus_tag="VFZ80_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEX6287366.1"
                     /translation="ATLESRIPFLHFFDGFRTSHEVSKIETIDVDQVRRLVDEDLIAA
                     HRLRALDPNRPVLRGSAQNPDVFFQAREAANRFYDDVPTVVSDTMKRFAQETGRGYDL
                     FDYAGSAEPETVLVIMGSGFGAAHEAVGRLVEEGRRVGVVQVRLYRPFSVDDFVASIP
                     ETAKRVVVFDRTKEPGAVGEPLYQDVVASLIEAGRRGLEVFGCRYGLSSKEFTPDMVA
                     GVVDEVEGPSPRRRFTVGIVDDVTGLSVPYDSSWWAEPEDVTTAVFYGLGSDGTVGAN
                     KNSVKIIGERTDLFAQGYFVYDSKKSGSRTVSHLRVSPRPIESTYLIRRADFVAVHHF
                     DIIREIDTLEVAAPGAKVLINTPFPAEMVWSELPIEAQRHIVDKDLEVWAIDADSIAT
                     EAGLGKRVNTVLQTCFFHLTDFMLTDEAIASIKEHIRMTYSKRGEVVVRRNDDAVDHA
                     VAGLARVSPGGVDSEVAIGLPVADDAPDFVKRVTAMVMAGRGDLLPVSALPVDGTFPT
                     DTARWEKRSIAAEIPIWDPDICIDCGRCALVCPHAAIRFKVFDAFEQIPEGFLWKDPT
                     GKDFPGKRVAIQVAPDDCTGCAICVEVCPAKSKEQVKHKALNMEAKEPHLERERRNFE
                     VFLNLPEIDRSEVNSASVKGSQLLQPLFEFSGACAGCGETPYLKLLTQMMGDRMVVAN
                     ATGCSSIYGGNLPTTPWATNGEGRGPAWSNSLFEDNAEFGLGMRLAADAQRDDAVLLV
                     TALAPELGGLAVEILELIENPDYSDTDGLIAHQRDLVDRLKARLADLEGPLPRRLEGV
                     ANSLIETSIWIVGGDGWAYDIGFGGLDHVMASGRNINILVLDTEVYSNTGGQASKSTP
                     RAAIAKFAAGGKTTGKKDLGQIAMSYGNVYVGQIAMGANPNQTVKTMAEAEAHRGVSL
                     VI"
BASE COUNT          486 a          875 c          879 g          508 t
ORIGIN      
        1 gatcaccaat gacacgccgc ggtgggcttc ggcttcggcc atggtcttca cggtctgatt
       61 cgggttggcg cccatcgcga tctgaccgac atacacgttg ccgtaggaca tggcgatctg
      121 cccgaggtcc ttcttgccgg tcgtcttacc cccggcggcg aacttggcga tggcggctcg
      181 tggggtggac ttggaggctt ggcccccggt gttggaatag acctcggtgt cgagcacgag
      241 gatgttgatg ttccgtcccg aagccatgac gtgatcgagg ccgccgaacc cgatgtcata
      301 ggcccatccg tcccctccca cgatccagat gctggtctcg ataagtgagt tggcgacacc
      361 ttcgagtcgc cgcggaagcg gcccctcgag gtcggcgagc cgggccttca gacgatcgac
      421 caggtcgcgc tgatgagcga tcaggccatc ggtgtccgag tagtcggggt tctcgatcag
      481 ctcgaggatc tccaccgcca gaccccccag ctctggagcg agggcggtca ccagtaacac
      541 ggcatcgtct cgctgcgcat ctgccgcgag cctcatcccg agcccgaatt cggcgttgtc
      601 ctcgaacagg ctgttcgacc acgccggtcc gcggccttcg ccgttggtgg cccatggtgt
      661 cgtggggagg ttgccgccgt agatcgagga acagccggtg gcattggcca cgaccatccg
      721 gtcgcccatc atctgggtga gaagcttcaa gtagggggtc tcgccgcacc cggcacatgc
      781 cccggagaac tcgaagaggg gctggaggag ctgggagcct ttgacagaag cggaattgac
      841 ctctgagcgg tcgatttcag gcagattgag gaagacttcg aagtttcgcc gctccctctc
      901 caggtgcggt tccttggcct ccatgttcaa cgccttgtgc ttcacctgct ccttcgattt
      961 ggccgggcac acctcgacac agatagcgca tccggtgcag tcgtcgggcg ccacctggat
     1021 ggcgacccgc ttccccggga agtccttccc cgtcgggtcc ttccacagaa agccttcggg
     1081 aatctgctcg aaagcgtcga agaccttgaa ccggatcgcg gcatgcgggc agaccagagc
     1141 gcaacgtccg cagtcgatgc agatgtcggg gtcccatatt gggatctcgg cggcgatcga
     1201 tcgcttctcc catctcgccg tgtccgtggg gaaggtccca tcgaccggca gggccgagac
     1261 cgggaggaga tcgccgcgac ccgccatcac catcgccgtc acccgcttca cgaagtcggg
     1321 tgcatcgtcg gcgacgggga gcccgatcgc cacctcactg tccaccccgc caggtgacac
     1381 cctggcgagc ccggccacgg catgatcgac ggcgtcgtca tttcgccgaa ccacgacctc
     1441 tccgcgcttg gagtaggtca tgcggatgtg ttccttgatc gaggcgatcg cctcgtcggt
     1501 gagcatgaag tcggtgagat ggaagaaaca cgtttggagg acggtgttca cccgcttgcc
     1561 gaggcctgct tcggtggcta tggagtcggc gtcgatagcc cagacctcca ggtccttgtc
     1621 gacgatgtgg cgttgggcct cgatcggcag ctcactccag accatctcag cggggaaggg
     1681 agtgttgatc aggacctttg ccccgggtgc agccacttcg agggtgtcga tctcccggat
     1741 gatgtcgaag tggtggactg cgacgaagtc cgcgcggcga atgagatagg tcgactcgat
     1801 cggtcggggt gacaccctca ggtgggagac ggtgcgcgat cccgacttct tggagtcgta
     1861 gacgaagtag ccctgggcga acaggtcggt cctctcgccg atgatcttca ccgagttctt
     1921 gttggccccg accgtcccat ccgagcccag cccgtagaag acggcggtgg tgacatcctc
     1981 gggctccgcc caccaggacg agtcataagg gacagatagg ccggtgacgt catcgacgat
     2041 gccaacggtg aagcgccgcc gtggcgacgg tccctccacc tcatcgacca cgccggccac
     2101 catgtcgggc gtgaattcct tcgacgagag gccataccgg cagccgaata cctcgagccc
     2161 acgccgtccg gcctcgatga gtgaggcgac aacgtcctga tagagaggct caccgacagc
     2221 ccctggttcc ttagtgcggt cgaaaaccac gactcgtttg gccgtctccg ggatcgaagc
     2281 gacgaaatca tccaccgaga aggggcggta gagacgcacc tggacgactc cgactctgcg
     2341 gccttcctcc acgagccgcc ccaccgcctc atgagctgct ccgaaccccg accccatgat
     2401 cacgaggacc gtctccggct cggccgatcc ggcgtaatcg aagaggtcgt agcccctccc
     2461 ggtttcctgg gcgaaccgct tcatcgtgtc gctgaccacc gttgggacat cgtcgtagaa
     2521 gcgattcgcg gcctcgcgcg cctggaagaa gacgtcaggg ttctgggcgc taccgcgaag
     2581 caccgggcgg ttggggtcga gcgcccgaag ccgatgggcg gctatcaggt cttcgtccac
     2641 cagacggcgc acctgatcca cgtcgatggt ctcgatcttc gacacctcgt gggacgtgcg
     2701 gaagccgtcg aagaagtgga ggaagggaat cctggactcg agggtggc
//
LOCUS       DASUTA010000392         3091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_115545, whole genome shotgun sequence.
ACCESSION   DASUTA010000392 DASUTA010000000
VERSION     DASUTA010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115545"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..722
                     /locus_tag="VFZ80_07765"
     CDS             216..722
                     /locus_tag="VFZ80_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019434704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX6287367.1"
                     /translation="MAEDAAPIRMEVVGVRVELPSNQPIVLLREEGGTRYLPIWIGAN
                     EATAIATAMEGVKPPRPLTHDLMRSILDAAGVLPVSVVITEMRDAVFFADLALDVSGE
                     ETKVSSRPSDAIALAVRTGTPVFAAAAVLDEAAVHFEDEDEEEEVARFREFLEEVTVE
                     DFLGGEQG"
     gene            735..1733
                     /locus_tag="VFZ80_07770"
     CDS             735..1733
                     /locus_tag="VFZ80_07770"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HEX6287368.1"
                     /translation="MSSRRQIVFGLGLLSIAVLVGTLGYVLIEGVPYFDAFYMVVITV
                     STVGFQQEFALSTMGRIWTMVVIGMGLGATLYTAIASIEYLVDLREVRRNRLMERDIA
                     RLSGHIVLCGFGRVGRGVWAALRDRELDVVIVEADGERAESARDGGALVIHGDATHND
                     VLELAGVASAEAVLACVADDSDNLVIALSVKSLRPELRVICRATELESERKLRLAGAD
                     AVVAPQAVGAERMAMMAIQPELAQVFDVVVSGNAVEFRVEELDIDPESSVAGKTIRDS
                     GLRQDTGALILAVEGVGDTVVVNPGPDQVLTPGERLVVVGTKDQVDRAAELLKSVR"
     gene            2014..2490
                     /locus_tag="VFZ80_07775"
     CDS             2014..2490
                     /locus_tag="VFZ80_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018215538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6287369.1"
                     /translation="MIDERELGGYRAPQVCNLVGITYRQLDYWARTGLIVPSLAQAHG
                     SGSQRLYSFTDVVQLKVIKNLLDAGMSLKKIRSAVEILRAEMDSDQPLADVTLLSDGQ
                     TIYAAHSDEEVVDVLRKGQGVFSIAIGPVQDQVQGAIHQLFPEENELPGVVEVGAP"
     gene            2658..2921
                     /locus_tag="VFZ80_07780"
     CDS             2658..2921
                     /locus_tag="VFZ80_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287370.1"
                     /translation="MPEDDLFIEITTMNQKLVTKKNKKVKLLKKYHPDVKCKIFYQRD
                     YLHLVVKYGLEEPDNLGDVVAPTRMPGEPEIVRLEDRGERLTG"
     gene            2918..>3091
                     /locus_tag="VFZ80_07785"
     CDS             2918..>3091
                     /locus_tag="VFZ80_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX6287371.1"
                     /translation="MNRSPLHDLNQRLGARFVDFGVWEMPVQYESVLAEHRAVREGVG
                     IFDVTHLGRFELSG"
BASE COUNT          574 a          924 c          993 g          600 t
ORIGIN      
        1 ggacgtgccc atcgagtatc gcgggttcga ggtgggtgat gagttcctgg tgggctacgg
       61 gctcgattgg cagggcttct atcgcaatct ccgctcgatc tgggcggttc tcgacatgga
      121 gaagctcgtc gccgagccga ctgcgctgct cagagagctc ggccacacct gatcgctgcc
      181 cttgggtctg gctccaccgg tgataacctc ggagcatggc agaagacgca gccccgatcc
      241 gcatggaggt ggtgggcgtg agggtggaac tgccctccaa tcagcccatc gtgctgttgc
      301 gcgaagaggg agggactcgc tatctcccga tctggatcgg cgccaacgaa gccactgcca
      361 tagccaccgc catggaaggc gtgaagccac cgcgaccgct gacccacgat ctcatgaggt
      421 cgatactcga tgcggccggg gtccttccgg tgagtgtcgt gatcaccgag atgcgcgacg
      481 ccgtgttctt cgccgacctc gccttggacg tgtcgggtga ggagaccaag gtctcttcga
      541 ggccttcgga tgcgatcgcc ctcgcggtcc gaaccggaac tccggtgttt gctgcagctg
      601 ccgttctgga cgaggcggcg gtgcacttcg aagacgagga cgaagaagag gaagtagctc
      661 gattccgcga gtttctcgag gaagtgaccg tcgaggactt cctcggcggc gaacaaggct
      721 gagcatcggg ccaggtgagc tcgcgacgcc agatcgtctt cgggttaggc ctgctttcga
      781 tcgcggtcct cgttgggacg ctcggttatg tgctcatcga gggcgtcccg tacttcgatg
      841 ccttctacat ggtggtgata acggtctcca ccgtcggatt ccaacaggaa ttcgccctct
      901 ccacgatggg tcgaatctgg accatggtgg tgatcgggat gggcctgggg gcgactcttt
      961 acactgcgat cgcctcgatc gagtacctgg tcgatctacg cgaggtgcga aggaaccggc
     1021 tcatggaacg tgacatcgcc cgactctcgg gccacatcgt gctctgtggt ttcgggcggg
     1081 tcggtcgggg agtctgggcg gcccttcgag atcgtgagct cgacgttgtg atagtcgagg
     1141 cggacggaga gcgcgccgaa agcgccagag acggtggggc tctggtgatt catggagacg
     1201 ccacccacaa cgacgtgctg gagctcgccg gcgtagcatc ggccgaggcg gtcttggcat
     1261 gtgtggctga cgactccgac aacctcgtca tcgctctctc ggtcaagtcg ctgcgtcccg
     1321 agcttcgcgt catctgccgg gccaccgagc tcgagtccga gaggaagcta cggcttgccg
     1381 gcgccgacgc ggttgtcgcc ccgcaggctg tcggggcgga gcgcatggcg atgatggcga
     1441 ttcagccgga gttggcacag gtgttcgacg tcgtggtcag cggcaacgcc gtcgagtttc
     1501 gcgtcgagga gctcgacatc gaccctgagt cctcggtcgc cggcaagacc atccgtgact
     1561 cagggctccg tcaggacacc ggagcgctga tcctcgccgt cgagggggtt ggcgacacgg
     1621 ttgtggtcaa ccccgggcca gaccaggtcc tgacccccgg ggagcgtctg gtcgtggtcg
     1681 gcaccaaaga ccaggtcgat cgggccgcag agttgttgaa gtcagtccgc taggccgcgc
     1741 cggtacccaa atccaacgac gttctgacct cattgcgcgt gcgcacagcg ttgcgttctc
     1801 cgcaacgtct tgcactcgga ggaccttgtc ttcttgccct tgcgcacagc gttgcgctat
     1861 ccgcaacgtc gtgcactcgg acccagctct ggcgacctgt cacaagcgcg tcgggggatg
     1921 tcgcgagttt tccacaggaa agcattgaca cgtggaggga ggtgcaagta gctttcccgg
     1981 ggcggagtta cactgatgta acccggagga caaatgatcg acgagcggga actgggcggc
     2041 taccgcgccc cacaagtgtg caatctggtc ggtatcacat accgacaact cgattattgg
     2101 gccagaaccg gcctcatcgt gccctcattg gcgcaggcgc atggatcggg gtctcagcgg
     2161 ctgtactcat tcaccgacgt cgtccagttg aaagtcatca agaacctcct cgacgcaggc
     2221 atgagcctga agaagatccg ctccgccgtc gagatcctcc gtgccgaaat ggactcggac
     2281 caaccccttg cggacgtgac gctgctcagc gacggccaga ccatctatgc cgcgcactcg
     2341 gatgaggagg tcgtcgatgt ccttcgcaag ggtcagggcg tgttcagcat cgccatcggg
     2401 cccgtccagg accaggtcca gggtgcgatc catcagttgt ttccagaaga aaacgagcta
     2461 cccggggtcg tcgaggtagg agccccctga ccactgaacc cctgaccgca cacgaggtcg
     2521 agttcgctca caacagtgag cgccagtttg ccctcctcct cgacttctac ggcgtcccct
     2581 gggagtacga gccgcgcagc tttcccatcg gattcgagga cgagaggcca gtcaagttct
     2641 tcactccgga cttctacatg ccggaggacg acctcttcat cgagatcaca acgatgaacc
     2701 agaagctggt gaccaagaag aacaagaagg tcaagctgct caagaagtat catcccgacg
     2761 tcaagtgcaa gatcttctat cagcgcgact atctccacct cgtggtgaag tacggcctcg
     2821 aggagccaga caatctgggg gacgtcgtcg ctccgactcg aatgccaggt gagcctgaga
     2881 tcgtccgtct cgaagaccgg ggcgaacgtc tcaccggatg aaccggtccc cacttcacga
     2941 tctgaaccag aggctcggag cgaggtttgt ggacttcggc gtctgggaga tgcccgttca
     3001 atacgagtcg gttctcgcgg agcatcgtgc ggttcgagag ggcgtcggca tctttgacgt
     3061 gacccatctc gggcggtttg agctatccgg a
//
LOCUS       DASUTA010000393         2527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_246351, whole genome shotgun sequence.
ACCESSION   DASUTA010000393 DASUTA010000000
VERSION     DASUTA010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246351"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VFZ80_07790"
     CDS             <1..647
                     /locus_tag="VFZ80_07790"
                     /inference="COORDINATES: protein motif:HMM:NF014804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX6287372.1"
                     /translation="VRVDSWITPGTSVSQYYDNLMAKLVVWGADRQSAINRGRRALKE
                     FEVSGVSTTIPAHLAVLDHEDFIEGRHHTKWMEEVVKVEGKTPSVAPALPEEEEVSKR
                     DITVEIGGRRFRVSYWAPEPGTGGQIRRRPPKLSSSGPSVASDGVVSAPMQGTIVKVS
                     VKAGESIKAGDPICVLEAMKMENEVSSPASGEVVDLRVEPGDTVAAGQVIAIVR"
     gene            complement(795..1874)
                     /locus_tag="VFZ80_07795"
     CDS             complement(795..1874)
                     /locus_tag="VFZ80_07795"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019050164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6287373.1"
                     /translation="MVEDTQTTEAFAGRIFDSVLGLIDSWSIYVGDKLGLYDALDQGS
                     LTRDELAGQTQTHARYIGEWLEQQVTTGILEVDDPSADPADRIYSLPEAHREVLVDRD
                     SLNYLTPFVRLMTAGGLQLPAILDAYRNGGGVPWERYGDDMRTGQGEMNRPWFVNAMA
                     TDWFPNAPEIHERLSRPGARVADIGCGEGWSSIAMARAYPNLTVDGYDIDEASVLAAR
                     RHADDAKLSDRLRFHHVDGEAIGEKGVFDVATLFECVHDMSDPIGVLGSVRELVKPDG
                     HVVVMDEAVADAFGDSTDDVERLMYGFSLFVCLPDGMSHPGSAGTGTVMRPSKLEEYA
                     MAAGFSGISVLPIENDLWRFYQLEL"
     gene            complement(1933..>2527)
                     /locus_tag="VFZ80_07800"
     CDS             complement(1933..>2527)
                     /locus_tag="VFZ80_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6287374.1"
                     /translation="VIRGAHALLAVGSVPLTEGLGLKAAGVSSDRGFVEVDEFQRTSA
                     PHIYAAGDVTGQMPLSSVASAQGRKIARHALDLPVQPIDYDKVTEAIFTEPEIASVGL
                     ADVVAAAQGRKVRTMKVPFSSNARSVLQDFTRGFVKVTSDPATGVVLGGTVVGHRASE
                     LIGIISLAVQARVTTQTLVETLLVHPSMIEAISDAAE"
BASE COUNT          470 a          802 c          819 g          436 t
ORIGIN      
        1 gggtccgcgt cgactcgtgg atcactccgg gaaccagtgt ttcccagtac tacgacaacc
       61 tcatggccaa gctcgtcgta tggggagccg acaggcaatc ggccatcaat cggggacgac
      121 gagctctcaa agagttcgag gtgtctgggg tgtcgacgac cattcccgcc cacctcgcag
      181 tgctcgacca cgaggacttc atcgaggggc gccatcacac caagtggatg gaggaggtcg
      241 tcaaggtcga aggcaagacg ccgtcggtgg cccctgccct tcccgaagaa gaggaggtgt
      301 cgaagcggga catcacggtg gagatcgggg gccggcgatt tcgggtctcg tattgggctc
      361 cggagccggg caccgggggc cagatccgca gacggccccc caagctctcc tcctccggtc
      421 cctcggtggc ctccgacggc gtcgtctccg ccccgatgca gggaaccatc gtgaaggtga
      481 gcgtgaaagc gggggagtcg atcaaggccg gcgacccgat ctgtgtcctc gaggccatga
      541 agatggagaa cgaggtctcc tcccccgcct ccggcgaggt ggtcgacctc cgcgtcgagc
      601 ccggcgacac cgtcgccgcc ggccaggtca tcgcgatcgt caggtagccg gagggtgagc
      661 aacagcgaac ccggggttgc tggcgccata tatggccaaa atcagctcat cttccggaaa
      721 ccatccgggc tagatctcgt agatgagcgg atatgtgccg tatacggcct gaggaacccc
      781 gggttcgctg ttggctacag ctcgagctgg tagaagcgcc agaggtcgtt ttcgatgggg
      841 aggaccgaaa tgccggagaa gccggctgcc attgcgtact cctcgagctt cgacggtcgc
      901 atgacggtgc ccgtgccggc actgcccggg tgggacatgc cgtcgggcag acacacgaag
      961 agcgagaagc cgtacatgag ccgctccacg tcatcggtgc tgtcgccgaa ggcgtccgcc
     1021 accgcctcat ccatcaccac cacatggcca tcgggtttga ccagctcccg gaccgatccg
     1081 aggaccccaa tgggatccga catgtcgtgg acgcactcga acagggtcgc gacgtcgaac
     1141 acgcccttct ccccaatggc ttcaccatcg acgtgatgga aacgcagccg gtctgacagc
     1201 ttggcgtcgt cggcatggcg gcgagccgcc aaaaccgatg cctcgtcgat gtcgtatccg
     1261 tcgacggtca gattcggata cgcccgggcc atcgcgatcg aagaccagcc ctcgccacat
     1321 ccgatgtcgg cgacacgggc tccgggtcgg gagagtcgct catgaatctc gggagcattg
     1381 ggaaaccagt cggtggccat ggcgttgacg aaccatgggc ggttcatttc gccctgtccg
     1441 gtccgcatgt catcgccata tctctcccac ggaaccccgc ctccgttgcg gtaggcgtcg
     1501 aggatcgccg ggagctgaag gcctccggcc gtcatcagcc gcacgaacgg cgtcaggtag
     1561 ttgagagaat ctcgatccac taggacttct cgatgcgcct ccggtaacga gtagatgcga
     1621 tcagcagggt cggcagatgg atcgtcgacc tcgaggatcc cggtggtgac ctgctgctcc
     1681 agccactcgc cgatgtagcg cgcgtgggtc tgagtctggc cggccagctc gtcacgggtc
     1741 agagagccct gatccagggc gtcgtacagc ccgagcttgt cacccacata gatcgaccag
     1801 ctgtcgatga ggccgagtac cgagtcgaag atccgcccgg caaaggcctc ggttgtctga
     1861 gtatcttcca ccatgtgaga cattctcctc gtcctctggt ggcgacgtcg ccgagaggac
     1921 gggcgcgaac ggctactcgg cggcgtcgct gatcgcttcg atcatcgatg ggtgcacgag
     1981 aagcgtctcc accagcgtct gggtggtgac ccgggcttgc acggcaagtg agatgatccc
     2041 gatgagctcc gacgcccggt gacccacgac ggttccaccg aggaccacac cggtggccgg
     2101 atccgatgtc accttcacga agccgcgggt gaagtcctgc aacaccgagc gggcgttcga
     2161 cgagaagggg accttcatcg tgcggacctt tcgtccctgc gcggcggcga cgacgtcggc
     2221 gagcccgacc gatgcgatct cgggctcggt gaagatcgcc tcggtgacct tgtcgtagtc
     2281 gatcggctgc acaggcagat cgagggcatg acgggcgatc tttcttccct gcgcagaggc
     2341 aacagagctg agcggcatct gaccggtgac atcgcccgcg gcatagatgt gtggcgccga
     2401 ggtgcgttgg aactcgtcca cttcgacgaa tccgcgatct gagctcacgc cggccgcctt
     2461 tagacccagc ccttcggtca aggggaccga tcccacggcg aggagcgcgt gcgctccccg
     2521 aatgacc
//
LOCUS       DASUTA010000394         2519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_634455, whole genome shotgun sequence.
ACCESSION   DASUTA010000394 DASUTA010000000
VERSION     DASUTA010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634455"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /locus_tag="VFZ80_07805"
     CDS             complement(<1..1464)
                     /locus_tag="VFZ80_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006370730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX6287375.1"
                     /translation="MISKVLVANRGEIARRIFRTCREMGIATVAVYSEADAGEPHMRE
                     ADESVLLPGVVPSDTYLNIEAVLAAAKETAADAIHPGYGFLSENAGFAREAIAAGFTW
                     IGPSAESIELMGSKLESKRLMEEAGVPVLPSVNPDGLSSIGVAAMSLGYPVLVKASAG
                     GGGKGMRVVSSETGLTDAVESARREAASAFGDDTIFLEKYLTAPRHIESQVFGDSEGR
                     VISLHERECSIQRRHQKIIEEAPSVALDPATRAAMGEAAVAAARAVAYVGAGTVEFLY
                     QNGSFYFLEMNTRLQVEHPVTEMVTGLDLVRLQIEIADGMPLADKGPRIEGHAIEARL
                     YAEDPLNDYLPVTGEFHRFEFPEFEGLRVDAGISAGSRVTVHYDPMVAKVIAHAPTRA
                     AAASLLATALRQARVHGSKTNRALLVRTLEHPEFLSGATDTHFLERNDVAELGSPLLD
                     EAGERLSAVGAAVADQAQARTQTTVLRSIPSGWRNSLS"
     gene            1568..2311
                     /locus_tag="VFZ80_07810"
     CDS             1568..2311
                     /locus_tag="VFZ80_07810"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HEX6287376.1"
                     /translation="MDGETRSRVDIEAESDHESPLQPTPGPSSPRRRFPIFSTTIIVL
                     VVGLALLTFWGVRTVINAIPTPGDIASVFEPEPYEEIGPATITAIRDLAQLTTVETVQ
                     YTIVEKGTDAGWLAWARGDSLRMFAVARIGAGVDLAQLTVRDIEVGDDGVVELTVPAA
                     TIQFVDPDEDATQVLERDVGIFTSGDPGLETDVRRIADEVLVDNAIDEGILDRAQTNA
                     RSVLSDFLMSLGYTDVVVEFEEPPADSES"
BASE COUNT          447 a          815 c          811 g          446 t
ORIGIN      
        1 gctcagcgaa ttcctccacc cggatgggat gctcctgaga acggtcgtct gcgttcgcgc
       61 ttgggcctgg tcggccacgg cggcacccac agcactgagc cgttcgcccg cctcgtcgag
      121 cagcggcgac ccgagctcag ccacgtcgtt gcgctcgagg aagtgagtgt cggtggcgcc
      181 cgagagaaac tccgggtgtt ccagtgtccg caccagcagg gcccggttgg ttttggagcc
      241 gtggactcgg gcttgccgca aagccgtcgc cagcagcgac gccgccgcgg cgcgagtggg
      301 tgcatgggcg atcaccttgg cgaccatcgg gtcgtagtgc accgttaccc gagacccggc
      361 gctgataccc gcatccactc tcaacccttc gaactcgggg aactcgaagc ggtgaaactc
      421 tcccgtcacc gggaggtagt cgttgagagg gtcctcggca tatagacgcg cctcgatggc
      481 gtgcccttcg attcgcggcc ccttgtcggc gaggggcatg ccgtcggcga tctcgatctg
      541 gagtcgcacc aaatcgagcc cggtgaccat ctcggtcacc ggatgctcca cctgtagacg
      601 cgtattcatc tcgaggaagt agaaggagcc gttctggtag agaaactcga cggtgccggc
      661 ccccacgtag gcgactgctc tggccgcagc cacagccgcc tcgcccatcg cggctctggt
      721 ggccggatcc agggccaccg aaggggcctc ctcgatgatc ttctgatgac gtctctgtat
      781 tgagcactct ctctcgtgga gagagatgac gcgtccttcg gaatcgccga agacctgact
      841 ctcgatgtgt cggggagcgg tgaggtactt ctccaggaag atggtgtcgt caccgaaggc
      901 gcttgctgct tcacgtcgcg ccgactcgac cgcgtcggtg agaccggtct ctgatgagac
      961 gacccgcatc cctttgccac caccgcccgc cgatgccttg accagaacgg ggtagccgag
     1021 cgacatggcc gcgactccaa tgctggagag cccgtccgga ttcaccgacg gaagaacggg
     1081 cacgccggct tcttccatca gtcgcttcga ctccagcttg gagcccatta gctctatcga
     1141 ctccgccgac ggaccgatcc atgtgaaccc ggctgcgatc gcctcccggg cgaagccggc
     1201 gttctctgaa aggaagccat atccggggtg tatggcgtca gctgccgtct ctttggccgc
     1261 agcgagcacg gcttcgatgt tcagataggt gtccgatggc acgacccccg ggagcaggac
     1321 cgattcgtcc gcctcccgca tgtgcggctc accagcgtcg gcttcggagt acacggcgac
     1381 cgtggcgatg cccatctcgc ggcatgtccg gaagatgcga cgggcgatct cgccccggtt
     1441 ggccaccaga accttggaga tcattggttc ccgaggctac cccgacgaac tcggttaggt
     1501 cccaagggga ctcggttgag aacgagggga ctcgggtaag gaccagggga aacgtaaaat
     1561 gcacgacatg gacggcgaga caaggtctcg ggtcgatatc gaggccgagt ccgaccacga
     1621 atcccctctt cagccgacgc caggcccttc atcgcctcgg cgtcgatttc ccatcttctc
     1681 gaccacgatc atcgtattgg tcgtcgggtt ggctctgctg acgttctggg gtgtccgcac
     1741 cgtcatcaac gccatcccaa cacctggcga tatcgccagt gtcttcgagc cggagcccta
     1801 tgaagagatc gggccggcga cgatcaccgc catccgtgac cttgcgcagc tgacgaccgt
     1861 ggagaccgtt cagtacacga tcgtcgagaa gggaaccgat gccggttggc tggcttgggc
     1921 tcgcggcgac tctctccgca tgttcgcagt ggcccgcatc ggggctggcg tcgatctcgc
     1981 ccagctgacg gtacgggaca tcgaggtggg cgatgacggt gtggtggagc tgacggtgcc
     2041 ggcggcgacg atccagttcg tcgatcccga cgaggacgcc acccaggtgc tggagcggga
     2101 tgtcgggatc ttcaccagcg gcgacccagg cctggagacc gatgtccgcc gcatcgcaga
     2161 cgaggtgctg gtggacaacg ccatcgatga ggggatcctc gatcgggccc agaccaacgc
     2221 ccgctccgtg ctgagcgatt tcctcatgag ccttggctac accgacgtgg tcgtcgagtt
     2281 cgaggaacca ccggcagact ccgaatcctg agtacgagcc ggaagcggcc ggccagacct
     2341 ctccgtgctc caacccatgg ttgtcggatt cgctgagcga agtgtggggc ccggagaacg
     2401 ccgcgaacca cacggcccac cgccggccag tgggcagacg agcccgccta catacggaag
     2461 acgccgaagc ggtcggttcc ctccaccgga ccggagtgga tggcggacag ggcgatgcc
//
LOCUS       DASUTA010000395         2305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_763174, whole genome shotgun sequence.
ACCESSION   DASUTA010000395 DASUTA010000000
VERSION     DASUTA010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763174"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..439
                     /locus_tag="VFZ80_07815"
     CDS             107..439
                     /locus_tag="VFZ80_07815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6287377.1"
                     /translation="MGITISNRELSNGWVAIAVEGEVDLATVEDLQTAIDQVLADSGD
                     HLVVDLTPSSFMDSTGLKVLVMSHRKFDDVGRSFAIAVDGGPISRLIDLSGVDKTIRT
                     VTSVEELG"
     gene            complement(436..726)
                     /locus_tag="VFZ80_07820"
     CDS             complement(436..726)
                     /locus_tag="VFZ80_07820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6287378.1"
                     /translation="MQSTLEPRYDDVLLVVSELVSNSVRHSESAEIDLIVRAQDGHIR
                     VEVSDEGPGFTPDAPRGEGLGLAIVEKLAERWGLVSGERFTVWAELSREVSA"
     gene            complement(850..1959)
                     /locus_tag="VFZ80_07825"
     CDS             complement(850..1959)
                     /locus_tag="VFZ80_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6287379.1"
                     /translation="MGDPAKLTLPALPDSAAKARAFMRETATLDSMRHAEADLLVTEL
                     IGNVISHSPDAEEIVIAVSQDREAGLVVSVEHAHPSAIDPEDTGVGFLLLERLSRSWG
                     IDHDGTNLSVWFTLRTPGTTSIAPDITDEELVGKMGDDPAAYSDELVRRHSDLVSSIS
                     RRYRGKGIDQDDLLQVANMALLKAIQRYDPKIGSIRPYAAATISGELKRLLRDRGWAV
                     RVPRSLQERSLEVARASEELSQTLNRAPEPGELAEKLDLTEEEVLEAMDARNAYVSSS
                     MDKPAESTGLTLLERLEDDDIRLVMADDRIVVEQAMRKLPERQQRILELRFNHDMTQS
                     EIADEIGISQMHVSRLLNRALESLKESIEIDAGSA"
     gene            complement(2025..>2305)
                     /locus_tag="VFZ80_07830"
     CDS             complement(2025..>2305)
                     /locus_tag="VFZ80_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287380.1"
                     /translation="TSGFGQRCEKVRRAATAISERSTERGSTGQVEERRRRLAEDAFD
                     ERATLRDEEHRLEARLGELPELASREGALPQRRAAAQTNLTHTPSLPR"
BASE COUNT          402 a          704 c          712 g          487 t
ORIGIN      
        1 ggcaagtgga cctataccga gatcggggat tgaggcatcg accggcacgg acgggcaggg
       61 gccgcccctt ctccagtgaa tcgatcgctg atagcatccc gactcgatgg ggatcacgat
      121 cagcaatcgg gagctctcca acggatgggt cgccatcgcg gtggagggtg aagtagatct
      181 cgccacggtg gaggacctgc aaacggccat cgaccaagtg ctggctgaca gtggcgacca
      241 cttggtggtc gacctgaccc cctcctcgtt catggattcg acgggtctca aggttctcgt
      301 gatgtcccat cggaagttcg acgatgttgg gcggtcgttc gccatcgcgg tcgatggggg
      361 gcccatttct cgtctgatcg atctcagtgg cgtggacaag acgattcgga ctgtcaccag
      421 cgtcgaagag ctcggctagg cggatacctc gcggctcaat tcggcccaaa ccgtgaatcg
      481 ctcaccggac acgagccccc atctttccgc cagcttctcc acgatggcca gccccaggcc
      541 ctcccctcgc ggcgcatcgg gggtgaatcc ggggccctcg tcactcactt ccaccctgat
      601 gtgtccgtcc tgggctctca cgatcaggtc tatctctgcc gattcgctgt gtcggacgct
      661 gttgctcacc aactccgaga cgacaagcag aacgtcgtcg tatcgaggtt cgagcgtcga
      721 ctgcataggg gcgagacgag atcgtgattc actaggagct ttcacgctgg actcgattcg
      781 tagagtcact tctctcatct cgtttctgct agatcagcaa taccccttct ccggaacgat
      841 aaacagcgat caggccgaac cggcgtcgat ctcgatcgac tctttgagcg actcgagggc
      901 cctgttcagg agacgggaca cgtgcatctg tgatatgccg atctcgtcgg cgatctccga
      961 ctgtgtcatg tcgtggttga agcgcagctc gaggattcgc tgttgtcgct ccgggagttt
     1021 cctcatggcc tgttcgacga ctatccggtc gtcggccatg accagtcgga tgtcgtcatc
     1081 ttcgagtcgt tcgagaaggg tcaagccggt cgactccgcc ggcttgtcca tggagctgga
     1141 cacgtaggcg ttgcgcgcat ccatggcctc gagtacctct tcctcggtca agtcgagctt
     1201 ttcggcgagc tcacctggtt cgggagcccg gttgagggtt tgcgacaact cctcggaggc
     1261 tctggccacc tcgagggacc gttcttgcag ggaacgaggg accctcaccg cccatccccg
     1321 gtcgcgaagg agccgtttca gctcaccgga gatcgtggcg gcggcatagg gccggatcga
     1381 cccgatcttg gggtcatagc gttggattgc cttgagtagt gccatgttgg ccacctgcaa
     1441 cagatcgtcc tgatcgatgc ctttgcccct atagcggcgc gagatggagc tgacgaggtc
     1501 gctgtgacgc cggacgagtt catcggagta ggcggccggg tcgtcgccca tcttcccaac
     1561 gagctcctcg tcggtgatgt cgggagcaat ggaggtggtt cccggcgttc gcaatgtgaa
     1621 ccagacagag aggttcgttc cgtcgtggtc gatgccccag ctgcgagaga gcctctcgag
     1681 aaggagaaag ccgacgccgg tgtcttcggg atcgatggca gaagggtgtg cgtgctctac
     1741 agacaccacc agccccgctt ctcgatcctg tgagacggcg atcacgatct cctcggcgtc
     1801 gggggagtgg gagatgacat tgccgatcag ctcggtgacc aataggtcgg cctcggcatg
     1861 gcgcatggag tcgagagtcg ccgtctcccg catgaacgca cgcgccttgg cagcggaatc
     1921 gggaagagcc ggcagagtca gcttggcagg atctcccatg aaccgaatct tacgaactct
     1981 ggcacctgtg cgagacgccc caccctggga tacggccgcc ctcatcatcg aggcagcgat
     2041 ggcgtatgag tcaggttggt ctgcgctgcg gcccgccgct gtgggagcgc tccttctctg
     2101 ctggccaact ctggcaactc accgagtcgt gcctccaagc gatgttcctc gtccctcaac
     2161 gtagcccttt cgtcgaatgc gtcctcagcg agccgacggc gtcgttcttc gacctggccg
     2221 gtgcttcctc tttcggttga tctctccgag atcgccgtgg cggctctgcg gaccttctcg
     2281 caccgttgtc cgaagcccga cgtga
//
LOCUS       DASUTA010000396         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1411991, whole genome shotgun sequence.
ACCESSION   DASUTA010000396 DASUTA010000000
VERSION     DASUTA010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411991"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /gene="argH"
                     /locus_tag="VFZ80_07835"
     CDS             <1..907
                     /gene="argH"
                     /locus_tag="VFZ80_07835"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX6287381.1"
                     /translation="MLAERNAEVLAPTYTHLQQAQVTSMGNHLLAYAWMAVRDIGRFS
                     DCALRLNESPLGAGASAGSTLPLDRGRVAESLGMSRPISNTLDAVGSRDFVAEYVFCC
                     AQAMVHLSRLAEEMILWASSEFGWMRLGDAVSTGSSALPQKRNPDIAELVRGRAAGVV
                     GDTQTILTLQKALPLSYNRDFQEDKRVVFHADDTLSYSLVAMPALLEGSSFSVPAPSP
                     DTAALPLAERLVIRGVPFREAHHVVGRLVHRMESAGKSLGDADDDDLQAIDDRFEAGD
                     LAALEFSVPDISEQISSLRRLLDAQ"
     gene            complement(939..1988)
                     /locus_tag="VFZ80_07840"
     CDS             complement(939..1988)
                     /locus_tag="VFZ80_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX6287382.1"
                     /translation="MTAPSYTRADFQTDQEVRWCPGCGDYTILASIQMFLAGLGRPRE
                     EFVFISGIGCAARFPYYVETYGMHSIHGRAPAIATGLATARPDLSVWVITGDGDSLSI
                     GGNHLIHALRRNVNLKIVLFNNQIYGLTKGQYSPTSEVGKATKSSPFGSIDYPFNPVS
                     LALGADATFVARTLDMDRNHTQEVLKKAYEHEGTAFIEVYQNCNVFNDKAFIQLTGKE
                     ERVHNRIDLQHGKPIVFDEGSKAIVLRGGEAEIVDSSSVDPKAILVHDETRPDPSVAF
                     ALSRLSHGPYGPTPLGIFRDVERDVYDIAVNAQVEAAREKMGPGDLDKLIRSHGTWTV
                     GPTSPNGSSNGSSGS"
BASE COUNT          332 a          625 c          645 g          399 t
ORIGIN      
        1 gatgctcgcc gagcggaacg ccgaggtgct ggcccccacc tatacgcatc tccagcaggc
       61 gcaggtgact tcgatgggta accatctact cgcatacgcc tggatggccg ttcgtgacat
      121 cggccgattc tccgactgcg cgctgcgact caacgagtct cccctcggtg caggtgcgtc
      181 ggccggttcg actcttcctc tcgaccgggg gagggtggcc gagtcgttgg gaatgtcgcg
      241 accgatatcc aacaccctcg acgccgtcgg gtcccgggac ttcgtcgccg agtacgtgtt
      301 ctgctgcgcc caggcaatgg tgcatctctc ccggctcgcc gaagaaatga ttctgtgggc
      361 cagctctgag ttcggctgga tgaggttggg tgacgcggtg agcaccgggt cttccgccct
      421 tccccaaaag cggaacccgg acatcgccga gctggtcagg ggcagggccg ccggcgtcgt
      481 cggcgacacc cagaccatcc tcactctgca gaaggctctt cctctctcct acaaccgcga
      541 tttccaggaa gacaagcgag tggtgttcca cgccgacgac accctctcct actcactggt
      601 cgcgatgccg gcgcttctcg aaggctcgtc gttctccgtc ccggcaccca gccccgacac
      661 cgcagccctt ccgctcgccg agcggctggt catccggggt gtccccttcc gcgaggccca
      721 tcacgtcgtg ggtcggctgg tccatcgcat ggagtcggcc ggtaagtcgc tcggtgacgc
      781 cgacgacgat gacctgcagg cgatcgacga ccgtttcgaa gccggtgatc tcgccgcgtt
      841 ggaattcagt gtccccgaca tttcagagca gatcagttcc ttgaggaggc tgttggacgc
      901 tcagtgaccg tcagcgcacc tgagtgcacc agactcgttc agctgccgga gctcccgttc
      961 gatgaaccgt tgggggaggt gggcccgacg gtccaggttc cgtgtgagcg gatcagcttg
     1021 tcgaggtctc cggggcccat cttctccctg gccgcctcca cctgagcgtt gactgcgatg
     1081 tcgtagacat cacgttcgac gtcgcggaag atgcccagcg gtgtcggtcc atagggaccg
     1141 tgggagagac gactgagggc aaaggcgacc gagggatcgg ggcgggtctc gtcgtgcacc
     1201 aggatcgcct tggggtcgac cgaggaggag tccacgatct cggcctcgcc tccccggagg
     1261 acgatggctt tcgagccctc gtcgaacacg atcggcttgc cgtgctggag gtcgattcgg
     1321 ttgtggaccc gctcttcctt gccggtgagc tggatgaagg ccttgtcatt gaagacgttg
     1381 cagttctggt acacctcgat gaaggccgtg ccctcgtgct cgtatgcctt cttcagcact
     1441 tcctgggtgt ggttgcgatc catatcgagg gtgcgggcaa cgaaggttgc gtcggcgccc
     1501 agcgccagtg acaccgggtt gaacgggtag tcgatcgagc cgaacggtga cgacttcgtc
     1561 gccttgccca cctccgacgt cggcgagtac tgacctttgg tcaatccgta gatctgattg
     1621 ttgaacagca cgatcttcaa gttgacgttg cgcctcaggg cgtggatcag gtgattgcct
     1681 ccgatggaga gtgagtcacc gtcgccggtg atcacccaaa ccgacaggtc ggggcgggcg
     1741 gtggccagac cggttgcgat cgccggggca cgtccgtgga tcgagtgcat cccgtaggtc
     1801 tccacgtagt acgggaagcg ggcggcgcag ccgatgccgg agatgaacac gaactcctcc
     1861 cggggacggc ccagaccggc gaggaacatc tggatcgatg ccaggatggt gtagtcaccg
     1921 catccggggc accacctgac ctcctgatcg gtctgaaagt ctgctcttgt gtatgacggg
     1981 gcggtcattt gaccatctcc a
//
LOCUS       DASUTA010000397         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_116820, whole genome shotgun sequence.
ACCESSION   DASUTA010000397 DASUTA010000000
VERSION     DASUTA010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116820"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VFZ80_07845"
     CDS             complement(<1..625)
                     /locus_tag="VFZ80_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX6287383.1"
                     /translation="MTTDVYETLRREVLARLDGTGLDPERDQDQVRDLLGATVEEYQQ
                     KAHLGDGLSLADPKATVDRLVRSVMGRSPLRELLERDDVEEIFIEGDKVTYLEAGGAL
                     RSSPVPTSESENRQTIEQLIAATDRRLDASNPVAQARILDGSARLSVVIPPIADRLSA
                     TVRRYALRRQTLESLVSLGSLTAQASDFLAAAMRASASIIVSGPPGAG"
     gene            complement(622..1716)
                     /locus_tag="VFZ80_07850"
     CDS             complement(622..1716)
                     /locus_tag="VFZ80_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287384.1"
                     /translation="MTPDIALAASAREWPDRLHRFLLDHGGGRIVDRVMSADQAVQSS
                     FDVLLIDDVCSFLSPRLVRDVNVRGGEMVGVFAPEDGSDAKRRLLECGISDVIETDAS
                     PEEFVEKVRQTLDHRSVAEVDQREDSSIIGIGVTGPSEGVGMTEVAISLAWALSDRVR
                     TALVDMDPVWPSVAQRLDLGLHPNIRTAIDHSLHNPDRLAESIHEVGEISVVGGRADG
                     STVSPVSRHDSMALLDALGAIADVVVADLGPLGQMEGGLLREFDTVIVVGTGGPVGVS
                     RLIRGVGDVRAVAADRPVLAVVNQPGRGAFRQSETLRELSSAFPDLAVLILPFDRKIA
                     EAAWDGELASGRSFRRAVGAMADVVVGVLT"
     gene            complement(1706..>2079)
                     /locus_tag="VFZ80_07855"
     CDS             complement(1706..>2079)
                     /locus_tag="VFZ80_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287385.1"
                     /translation="SEGAVVDTSSLSEPGEGSGLRTMSMPIDREHAAGGSLVAGDRVD
                     VISVVDGVPGFVATNLEVTGVAEEASGGIGAVSAYHVVLSVSAEQALALAAAIDSGSL
                     EIIRSTGAEAIDPLTTKSDDS"
BASE COUNT          404 a          691 c          640 g          344 t
ORIGIN      
        1 tgcccgcccc cggcgggccg gagacgatga tcgaagcact ggcccgcatc gcagccgcca
       61 ggaaatcgga tgcctgggct gtcagggatc cgagcgagac caacgactcc agcgtctggc
      121 gtcgcagggc atatcgccga accgtcgccg agagccgatc ggcgatgggc gggatgacga
      181 cactgagccg ggccgagccg tcgaggattc gggcctgcgc aacaggattg gaggcgtcga
      241 gacgacgatc ggtggccgca atcagctgct cgatggtctg tcggttctcg gactcggacg
      301 tgggaactgg ggaggagcgc aaggccccgc cggcttcgag ataggtcacc ttgtcgcctt
      361 cgatgaagat ctcctcgaca tcgtcgcgtt cgagcagttc acgcaatggg ctgcgtccca
      421 tcaccgaccg gaccaaccga tcgacggtcg ccttggggtc ggcgagcgat aggccgtccc
      481 cgagatgagc cttctgctgg tactcctcga cggttgcacc gagaagatcg cgaacctggt
      541 cctgatcacg ctccgggtcg agaccggtgc catcgagacg agcaagtacc tcccgtcgca
      601 gggtttcgta gacatccgtg gtcacgtcag gacccccacc acgacgtctg ccatggcacc
      661 cacagcccgc cggaacgaac gacccgaggc cagctcgccg tcccaggcgg cttcggcgat
      721 cttcctgtcg aaggggagga tcaggactgc caggtcgggg aaagctgagc tcagctcgcg
      781 aagtgtctcg gactgcctga aagccccgcg cccgggctga ttgaccacag cgagtaccgg
      841 acgatcggcg gccacagccc tgacatctcc cactcctcgt atgagacggg acacacccac
      901 aggacctccc gttccgacga cgatcacggt gtcgaattct ctgaggaggc caccctccat
      961 ttgccccagt ggaccgagat cggccaccac cacatcggcg atggcgccga gagcatcgag
     1021 gagagccatc gagtcatgcc ggctcacagg agagacggtg ctgccgtccg cccggccacc
     1081 aacaacgctg atctcaccga cctcatgaat cgattcagcg agccggtcgg ggttgtgcag
     1141 cgagtggtcg atggcggttc gtatgttcgg gtgcagaccc aggtcgagac gttgcgccac
     1201 ggatggccac accggatcca tatcgaccag ggccgtccgg actcgatctg ataacgccca
     1261 ggccagcgaa atcgccacct cggtcatccc gacgccttcg gacgggccgg taacaccgat
     1321 gccgatgatc gacgagtcct ctcgctggtc cacttcagcg accgatcggt gatccagggt
     1381 ctggcgaacc ttctcgacga attcttccgg cgatgcatcg gtctcgatga catcggagat
     1441 cccacactcc agcagtctcc gtttggcgtc tgatccgtct tccggtgcga agacgccgac
     1501 catctcaccg cccctgacgt tgacgtctct gaccaaccgt ggcgacagaa acgagcagac
     1561 gtcatcgatc agcaagacat cgaaagaaga ttgcaccgcc tggtcggcac tcatgacgcg
     1621 gtcgacgatt cgtcccccac cgtgatcgag gaggaagcgg tgcagtcggt cgggccactc
     1681 ccgtgctgat gctgccaggg cgatatcagg agtcatcgct cttcgtcgtc aggggatcga
     1741 tggcctcggc tccggtcgat cgaatgatct ccagtgagcc cgagtcaatc gctgctgcca
     1801 gggccaacgc ctgttcggca ctcaccgaga ggactacgtg ataagcggaa acggcgccga
     1861 ttccaccaga agcctcttcg gcgacgccgg tcacctcgag gtttgtcgcc acgaacccgg
     1921 gcactccgtc gaccacggag atcacatcga cccggtcacc ggcgacgagg gagccgccgg
     1981 cggcatgctc ccgatcgatc ggcatgctca tcgtccgcaa gccgctacct tcgccgggct
     2041 cagaaagtga gctcgtgtcg accacggccc cctcagaga
//
LOCUS       DASUTA010000398         2419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_892766, whole genome shotgun sequence.
ACCESSION   DASUTA010000398 DASUTA010000000
VERSION     DASUTA010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892766"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VFZ80_07860"
     CDS             <1..911
                     /locus_tag="VFZ80_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX6287386.1"
                     /translation="DRPELHDVVREWRAVLDEYEDKVMVAEAWIPSWPRLAMYVRPDE
                     YHQTFAFQFLRAPWDPEAMRSTIHDALAATGSVGSLPTWVLSNHDVMRQVTRYGLADD
                     VDERAWLLDGDPSSFDYELGLRRARAAALLMLALPGSVYLYQGEELGLPEVSDLPEGV
                     IDDPVWERSGKTEKGRDGCRVPIPWTSEGDSFGFGANGSWLPQPDQWSELSVESQRGD
                     DGSTLELYRNAIRIRSALLRHDEELEWMDLGTDVVAFARGGAMTCVVNFGTDPIPVPD
                     GEVVLISAPMSNGLLPGNSAAWIVSS"
     gene            complement(920..1963)
                     /locus_tag="VFZ80_07865"
     CDS             complement(920..1963)
                     /locus_tag="VFZ80_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011981854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6287387.1"
                     /translation="METRRCGGSSLEVSVFGLGTMTFGAESDETTSHEILDAYYESGG
                     RFIDTADVYTQGVSEEIIGLWLQRRKPDDIVIGTKARFAMGEGPDESGAGRRHLVKAL
                     EASLTRLGVEAIDLYQMHAWDPHVELAETLETLNGFVTDGKVRHIGISNYMGWQLERA
                     IQLSAQNDWAPIVSAQPQYNLLAREIEWDLLPVCLENDIGVLPWSPLGGGWLTGKYSP
                     EHRPSGVSRLGEDPGRGVEAYDLRNTDRTWEVLAAVRRVAEGLGVSMTQVALNWVRNR
                     PGVSSVLLGCRTVEQLRDNLGALTWELDTDELTLLTEVSAPGMPSYPHGFLEKLADVD
                     VWDRLGTRAQPVL"
     gene            2045..2416
                     /locus_tag="VFZ80_07870"
     CDS             2045..2416
                     /locus_tag="VFZ80_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287388.1"
                     /translation="MLEWKKVDDSWFGNGFQVRRIAPSLWSLEHVSGGDAVDVSDPIA
                     ELSTLDACKYEAEARHRAGEDSARRRRLGAVAIGGWSLAVLSVNPILFIAAGVVGTAA
                     LLEIAAMYFDDRTGSVRDFVQ"
BASE COUNT          432 a          765 c          741 g          481 t
ORIGIN      
        1 gggaccgccc ggagcttcat gatgtggtgc gggagtggcg ggcagtgctc gacgagtacg
       61 aagacaaggt catggtcgcc gaggcgtgga tccccagctg gccccgcctg gcaatgtacg
      121 tgcggccgga tgagtaccac cagacatttg cctttcagtt tctgagggcg ccgtgggatc
      181 ccgaagcgat gcgttcgacg atccacgacg ctctcgcggc cacggggtcg gtgggctctc
      241 tgccgacctg ggtcctctcc aatcacgatg tcatgcgcca ggtcacccga tacggacttg
      301 ccgatgacgt ggacgagcga gcgtggctgc tcgacggtga cccttcgagt ttcgactacg
      361 agttggggct tcgtcgtgcc cgggcggccg ccctactcat gttggccctt cccgggtcgg
      421 tatacctcta tcagggggag gagctcgggc taccagaagt gagcgatctt cccgagggtg
      481 tgatcgacga cccggtgtgg gagaggtcgg gcaagaccga gaagggtcgc gatggttgcc
      541 gggttcccat tccgtggaca tccgaggggg attcgttcgg ctttggcgcc aacggctcat
      601 ggcttcccca accggaccag tggtctgagc tctctgtcga gagccagaga ggtgacgatg
      661 ggtcgaccct ggagctctat cgcaatgcga tccgaatacg cagcgctctt ctccgccacg
      721 atgaagagtt ggagtggatg gatctcggga ccgatgtcgt cgccttcgcc cgcggcggcg
      781 ccatgacctg tgtcgtcaac ttcggcaccg acccaatccc ggttcccgac ggtgaagtcg
      841 tcctgatcag cgctcccatg agcaacggtc ttcttccggg gaactcagcg gcctggatcg
      901 tgtcgagctg acgagagact cagagcaccg gctgcgcccg ggtgccgaga cggtcccaga
      961 catccacgtc tgccaacttc tccaagaacc cgtgggggta actgggcata cctggtgcgc
     1021 tcacttcggt gagcagcgtc aactcgtcgg tgtcgagctc ccatgtcaac gcaccgaggt
     1081 tgtcccgcaa ctgctcgacg gttcgacatc ccaagagcac cgagctcacc cccggtctgt
     1141 tccgcaccca gttgagcgca acctgggtca tcgacactcc caaaccttcg gcaacccttc
     1201 tcacggcagc gagcacctcc caggtccgat ctgtgttcct cagatcgtat gcctcgactc
     1261 cccgtcccgg atcctccccg agccgggaga caccgctcgg acggtgctcc ggcgagtact
     1321 taccggtgag ccagccacca cccagtggag accagggcag caccccgatg tcgttttcca
     1381 gacacacagg caggagatcc cattcgatct cacgcgccag caagttgtac tggggttgcg
     1441 cagagacgat cggcgcccag tcgttttgtg ctgacagctg aatcgctcgc tccagctgcc
     1501 atcccatgta gttggagatg ccgatgtgtc gcaccttgcc gtcggttacg aacccgttca
     1561 gtgtctcgag ggtctcggca agctcaacat gcggatccca ggcgtgcatc tgatacaggt
     1621 cgatcgcctc gaccccgagc cgggtcaggc tcgcctccaa tgccttgacc agatgccggc
     1681 gaccggcacc gctttcgtca ggaccctcac ccatggcgaa tctggccttg gtcccgatca
     1741 cgatgtcatc cggcttacgg cgttgcagcc aaagcccgat gatctcctcg gagacgcctt
     1801 gggtgtacac gtcggcggtg tcgatgaagc gacctccgct ctcgtaatag gcatcgagaa
     1861 tctcgtgtga cgtggtttcg tccgactcgg cgccgaaggt catcgttccc agcccgaaga
     1921 ccgagacctc gagcgaggag cctccgcatc gtcttgtctc cattgcaacg agactacgcg
     1981 ctctcatcca acacttaaca agccgtcgat tcctcggtag attcgatagt gaaaggagca
     2041 ccaaatgctc gaatggaaga aagtcgacga ttcgtggttc ggcaatggat tccaggtcag
     2101 gcgaatcgct ccctcgctct ggtctctgga gcatgtcagc ggcggtgatg ctgtcgacgt
     2161 gtccgatccg atagcggaac tgagcactct cgacgcatgc aagtacgagg ccgaagcgcg
     2221 tcatcgcgcc ggggaggact cggcgagacg ccggcgtctc ggagctgtcg ccatcggtgg
     2281 atggtctctg gccgtgctgt ctgtcaatcc gattctgttc atcgcggccg gtgtcgtcgg
     2341 gaccgctgcg ctgctcgaga tagccgccat gtatttcgac gatcgcaccg ggagcgtccg
     2401 cgacttcgtc cagtagcgc
//
LOCUS       DASUTA010000399         3888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1672714, whole genome shotgun sequence.
ACCESSION   DASUTA010000399 DASUTA010000000
VERSION     DASUTA010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672714"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /locus_tag="VFZ80_07875"
     CDS             complement(<1..1385)
                     /locus_tag="VFZ80_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6287389.1"
                     /translation="MDGPLAAQLHLEEFGADFLREFVNLLLLPNVTGQVVDLRANARE
                     IASRFAARGATMEVVELEGASPVVIGELRCERPTATIGVYVHYDGQPVDAENWTYEPF
                     SATFLSDAIHNGGLPLSLPEAGETIDSNSRIYARGASDDKTPLAVLPAALDALDEAGL
                     GRTVDLIFLFEGEEESGSPNLERYMTDLATRLEADAWLLCDGPVHQSRRPQVSFGARG
                     YSGFELTFYGPERELHSGHYGNWVPNPVLDLARFLATCKDDSGRVTVPGFYDDTVPVS
                     DADRAAIDALPAIEESLQEELGFGGAEVEGTTYADRLMLPSFNVRGIRSAEVGEGARN
                     VIPARATASVDMRLAAGDDPNRMVDRVESFLRSSGYKVLDREPTREERRNHRRLARLD
                     RLPGYRAARIPMESSLATTLLDVCHLASGEAVVALPTFGGSIPLYLFEDILGSPVAIL
                     PIANHDNNQHAP"
     gene            1466..2590
                     /locus_tag="VFZ80_07880"
     CDS             1466..2590
                     /locus_tag="VFZ80_07880"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX6287390.1"
                     /translation="MSRVRAATAQGATFVELFFDLVFVYAVTQLTSLVLHDLTWQGVG
                     EAALLFWLVWWAWTQFTWTLNLADTEHVWVRLPTLAATAVAFFLAQSVPDAFGDAGAW
                     FAISYVVVRIIGLGVQAWVIGSDQLESLVRWASLSLVGMGLVLIGGIVDPEVRVWLWL
                     GALVADLVSAFRAGRSVWVLEPGHFAERHGLIVIIALGESLIAAGVASSEVERGLMFA
                     VTTAGAVVATCALWWTYFGTLHGRLEERMAEQTDTTRGAFARDVFSFWHAAVVAGVIG
                     VAVAFEEAIAHPDEALSIGAALSLTVGSALFLGGLASSAARAAVGQAVLPRVLVALIA
                     LAATPLIPQVPAWCALWGLAILTMAMDVFESRRSDRLSPV"
     gene            complement(2594..3610)
                     /locus_tag="VFZ80_07885"
     CDS             complement(2594..3610)
                     /locus_tag="VFZ80_07885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6287391.1"
                     /translation="MSLGFGLLSAQLNPGETDWSRVYEETIRLAVDAERLGFGSVWTT
                     EHHFVDDGYMPSLAVVSAAIAARTSHIEIGTGVILAPLHHPLRLAEDAATVSLLSGGR
                     FILGLGLGWSETEFEGLGSDMSTRGAAMEEILELLPKAWTGEPFHHEGRVYRFPELAV
                     RPKPSRPIQVLVGGGAETAVRRAARLADGIFANVPKDQFLQQLAWIEDECEQVGRDPD
                     DLRIVHYSVMLPAGSQQEAFERYTDHLWQMMWKYSDMEDSTNRPGPPPSAPPFDEAKR
                     DRLFGRATIAGSGEEIVEYLHDIRERANFDVEFAARSYFPTLSYEAQVELMEQLATEV
                     SPHV"
     gene            complement(3607..>3888)
                     /locus_tag="VFZ80_07890"
     CDS             complement(3607..>3888)
                     /locus_tag="VFZ80_07890"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX6287392.1"
                     /translation="VENVFDLGYRRCEWKCDALNEPSRRAAIRLGFTYEGTFRQATHY
                     KGRNRDTAWYSIIDREWPELADAFTRWLAEDNFDVDGRQRSSIEEMRSR"
BASE COUNT          648 a         1210 c         1283 g          747 t
ORIGIN      
        1 ggggcgtgct ggttgttgtc gtggttggcg atgggcagga tcgccaccgg cgacccgagg
       61 atgtcctcga agaggtagag gggaatcgag ccgccgaacg tggggagggc cacgacggct
      121 tcacccgagg ccaggtgaca cacgtcgagc aacgtcgtcg caagggagga ctccatgggg
      181 attcgagcgg cgcggtagcc ggggagtcga tcgaggcggg ccaggcgccg gtgattgcgg
      241 cgctcctcac gtgtcggctc gcggtccaga accttgtaac cggaggaacg aaggaagctc
      301 tccacacggt cgaccatgcg gttggggtcg tcgcctgctg ccagtctcat gtctaccgaa
      361 gccgtggccc gggcagggat cacattgcgg gctccctcgc ccacttcggc agatctaatg
      421 cctcgaacgt tgaagctggg gagcatgagg cgatcggcat atgtcgtgcc ctctacttcg
      481 gcaccgccga aaccgagctc ctcctgcaag gactcctcga tggcgggcag ggcatcgatg
      541 gccgcccggt cggcatccga gacggggacg gtgtcgtcgt agaagcccgg aaccgtgact
      601 cggccggagt cgtccttgca tgttgcgaga aaccgggcca gatccaggac ggggttgggc
      661 acccagttgc cgtaatggcc ggagtgcagc tctctctccg gtccgtagaa ggtgagctcg
      721 aagcccgaat agccgcgcgc cccaaacgag acctgtgggc gacgactctg atgaacaggc
      781 ccgtcgcaca ggagccatgc atcggcttcg agtctggtgg ccaggtcggt catatagcgc
      841 tccaggtttg ggctacctga ctcctcctcg ccttcgaaca agaagatgag gtcgaccgtc
      901 ctccccagcc cggcttcgtc gagggcatcg agggcggccg ggagcacggc aagaggagtc
      961 ttgtcgtccg aggctccccg agcgtagatc ctcgagttgg agtcgatggt ctcgccagcc
     1021 tcggggagtg acagcggcaa tccgccgttg tggatcgcat ccgagagaaa cgtcgccgag
     1081 aagggctcgt acgtccagtt ctcggcgtcg accggctgcc cgtcgtagtg gacgtagacg
     1141 ccgatcgtgg cagtgggtcg ttcgcagcgc agctcgccga tgactactgg gctcgctccc
     1201 tccaactcga cgacctccat cgtcgcacca cgcgccgcga accgggaggc gatctcgcgc
     1261 gcgtttgccc gaaggtctac gacctgtccg gtgacattgg gaagtagcag gaggttcacg
     1321 aactcacgga gaaagtccgc accgaattcc tcgagatgca gctgggcagc aagggggcca
     1381 tccatcgaca agagcctaaa gccgcgcgct gcggcgatgg gaagctgtgc atccggcgaa
     1441 cgcgattcat ttaggctcgc cggccatgtc gcgtgtccgc gccgccaccg cacagggtgc
     1501 gacctttgtc gagctgttct tcgacctcgt gttcgtctat gcggtcactc agctcacgtc
     1561 gttggtgctg cacgatctca cctggcaagg ggtcggcgag gccgctctgc tgttctggtt
     1621 ggtctggtgg gcgtggactc agttcacctg gaccctcaac ctggcggaca cagagcacgt
     1681 ctgggtccgg ctgccgacgc tcgcagccac agctgtggcc ttctttctcg ctcaatcggt
     1741 gcccgacgcc tttggcgacg ccggcgcctg gtttgcgatc agctatgtgg tcgttcgcat
     1801 cattggattg ggcgtccaag catgggtgat cggatccgac cagctcgagt ctttggtcag
     1861 gtgggcgtct ctatcgctgg tcggcatggg cctggtcctg atcggaggga tcgtagaccc
     1921 cgaagtccgg gtgtggctct ggcttggggc gctggtggcc gacctggtca gcgcatttcg
     1981 cgccggacga agtgtgtggg ttctcgaacc gggacacttt gccgagcgtc acggtctcat
     2041 cgtgatcatc gcactcggcg agtcgctgat tgcggccggg gtggcttcga gtgaagtcga
     2101 gagagggctg atgttcgcag tcaccaccgc tggagcggtc gtcgcgacct gtgccctgtg
     2161 gtggacctat ttcgggacac tgcacggtcg cctcgaagag cgaatggcgg agcagaccga
     2221 caccactaga ggagcatttg ccagagatgt cttctcgttc tggcatgccg ccgtcgtcgc
     2281 cggtgtcatc ggtgtcgctg tcgccttcga agaggcgatc gcccatccag acgaggcgct
     2341 gagcatcggc gccgccctct ccctcacggt cggatcggcg ctgtttctgg gcggcctcgc
     2401 ctcatcggcg gctcgggccg cggtgggtca ggccgttctt ccgcgcgtcc tcgtcgcact
     2461 catcgccctg gccgccactc cgctcatccc ccaggtcccg gcgtggtgtg cgctttgggg
     2521 tctcgccatc ttgaccatgg cgatggacgt cttcgagtca cggcgtagcg atcgcctcag
     2581 ccctgtctga cagtcatacg tgtggagaca cctcggtggc gagctgttcc atcagctcga
     2641 cctgagcctc gtacgaaagc gtcgggaagt agctgcgggc cgcgaattcc acgtcgaagt
     2701 tggcccgctc ccggatgtcg tggaggtact cgacgatctc ctcacccgac ccagcgatcg
     2761 tggcccggcc gaagaggcgg tcacgtttgg cctcgtcgaa tgggggagcc gagggtggag
     2821 gaccgggtcg gttggtggaa tcctccatat cggagtactt ccacatcatc tgccacaggt
     2881 gatcggtgta gcgctcgaag gcttcctgtt ggctgcccgc gggcaacatc accgagtagt
     2941 ggacgatcct gagatcgtcc gggtctcgac ccacttgctc acactcgtct tcgatccagg
     3001 cgagctgttg caggaactgg tctttcggaa cgttggcgaa gatcccgtcc gcgagcctgg
     3061 ccgctctgcg gacggccgtc tcggcacccc cgccgaccag cacctggatg ggccgagaag
     3121 gcttgggtcg caccgccagc tcggggaatc tgtaaacccg gccctcgtga tgaaacggct
     3181 cacccgtcca ggctttggga agcaactcga ggatctcctc catggcggct ccccgtgtgg
     3241 acatatccga accgagaccc tcgaattcgg tctcactcca gccaagaccc agcccgagga
     3301 tgaaccgacc tccgctcagc aaactcacgg tggcggcatc ttcggcgaga cgaagcgggt
     3361 ggtggagagg tgccaggatg acgccggtcc cgatttcgat gtgtgaggtc cgggccgcga
     3421 tggcggcgct gaccacagcg agggaaggca tgtacccatc atcgacgaag tggtgctcgg
     3481 tcgtccagac cgagccgaag ccgagtcgct cggcatcgac cgccaaccgg atcgtctcct
     3541 cgtagacgcg cgaccagtcg gtctcgccgg gattcagttg tgcgctgagc aggccaaagc
     3601 cgagactcat ctcgatctca tctcctctat cgacgatctc tgccggccgt cgacgtcgaa
     3661 gttgtcctcg gccagccacc tggtgaacgc gtcagccagc tctggccatt cccgatcgat
     3721 gatggagtac caggcggtgt cgcggttcct ccctttgtag tgggtggcct gacggaacgt
     3781 gccttcgtag gtgaaaccga gtcggatcgc ggctcggcgg gacggttcgt tcagtgcatc
     3841 acacttccac tcgcatcgcc ggtaacccag atcgaacacg ttctcgac
//
LOCUS       DASUTA010000400         4023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1543513, whole genome shotgun sequence.
ACCESSION   DASUTA010000400 DASUTA010000000
VERSION     DASUTA010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543513"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VFZ80_07895"
     CDS             complement(<1..797)
                     /locus_tag="VFZ80_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(555..557),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6287393.1"
                     /translation="MKVKRDYARLTRPLVRKDGRLQPVSWDEAMATAAEGLSRYRGES
                     FGMFSCSKATNEVNYIAQKWARMVMASNNIDSCNRTUHAPSVAGLAAVFGAGGGTSSY
                     QEIEETDLIVMWGSNAREAHPIFFHHVLKGVRNGARMFAVDPRRTSTAAWADVWLGID
                     VGSDIALANAVGREIIAQGLHNREFIEAATEDFDAYAASVDDYTLERASQITGVPASA
                     IQELAHAYGSAETAQICWTLGITEHHNATDNVLALINLALLTGHVGR"
     gene            complement(853..2187)
                     /locus_tag="VFZ80_07900"
     CDS             complement(853..2187)
                     /locus_tag="VFZ80_07900"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6287394.1"
                     /translation="MSETVRSLVRRYYVYAGLYTLASSVIWGVNTLFLLDAGLTISEV
                     FIANAAWSLGTTLFEIPTGVVADTIGRRASFLLSVVVLALTTLAYVGLAQIGGSVVAF
                     VAISLLIGLGFTFYSGAMEAWLVDGMHSLGYTEEMDRIFSRGQAISRAAMIIGTVGGG
                     ILGQVDLAIPFLVRSGLLIALFVLAFVGMHDLGFEPRRVTLSQLPSEAMAIGRAGVRF
                     GWADRSLRLLMLGTAAQMGVFAWAWYAWQPYFLELLERDVVWVAGVVAALLSLSMMLG
                     NGIVEIVSRWCGRRSTIFLWSSAVFSLALIGVGLASTFVAALVLLCVSGVAFGAQSPV
                     RQAFIHRVAPSRQRATVVSFDSMITGGASVAGQTGLGYVAEGQGFAAGYVACGAIALT
                     AMPFYALVRRIGADADRFAGRRHEFAPTPRGVPHVLQVAGDVSTEAEGEEGV"
     gene            complement(2238..3491)
                     /locus_tag="VFZ80_07905"
     CDS             complement(2238..3491)
                     /locus_tag="VFZ80_07905"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6287395.1"
                     /translation="MSVPFVLPALLADPAPPEDAYALRNARIFDGSGSDPFEGDVFIA
                     NGRVQTEAASGETTSIDLDGRFVMPGLIDVHTHLSLQPQVSMTEGMEKLRPEVNGHLV
                     AAGLRRALRMGITTVRDVGAWGDEILAIRQAVRYGAFLSPRLLICGRIVSATSPGGRH
                     FEGMYREADGPDEMRKAAREQIRRGADLVKIMMTGARSVEYENPAPPQVTREELAALV
                     DEVHRQGFRVAAHCEGLEGTIMAIEEGVDTIEHGFYLNQRPEYLARMAETGQTLVPTI
                     TFLHDVVTGRHEAWSEALVERGEYNVSQAEATLVAAIEAGVNIAMGFDSTPEEKAAGE
                     LAVMVDAGMTTSDALVAATAAGARALGLDDLIGRIADGMIADLVIVEGDPLHDVSLLT
                     DPQAMQLVIRGGHLAHVAPHLDPEG"
     gene            3538..>4023
                     /locus_tag="VFZ80_07910"
     CDS             3538..>4023
                     /locus_tag="VFZ80_07910"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6287396.1"
                     /translation="MRSEISMGQIPASAEFVIVGAGVHGMSTAWHLAMERGSGDGIVV
                     LDKTAPGAGATGIACGCVRNFYMTEPIHALIRESVDVWNYDPVLLGFQQVGYVSCGEE
                     NQISEYEKIHKSQNSVGYTSDLYVGQDARDFLTSVWPDFRHHSIDVVIHEKLSGYAGT
                     AQ"
BASE COUNT          798 a         1320 c         1235 g          670 t
ORIGIN      
        1 tagcgaccga cgtgaccggt gagcagggcc aggttgatga gagcgagcac gttgtcggtg
       61 gcgttgtgat gctcggtgat cccgagtgtc caacagatct gagccgtctc agccgagcca
      121 tatgcgtgag ccagctcctg gattgccgat gccggaacgc cggtgatctg cgaagcccgc
      181 tccagggtgt agtcgtcgac cgatgccgca taggcgtcga agtcctcggt ggcagcctcg
      241 atgaactccc tgttgtgaag gccctgggcg atgatctccc tgccgactgc gttggccagg
      301 gctatgtcgg agccgacatc gatgccgagc cagacatcgg cccaggcagc ggtggaagtg
      361 cggcgggggt cgacggcgaa catgcgggcg ccattgcgaa cccctttgag gacgtggtgg
      421 aagaagatgg ggtgcgcttc gcgcgcgttc gagccccaca tcacgatgag atcggtctcc
      481 tcgatctcct ggtacgagct ggtgccaccg cccgctccga acacagccgc cagaccggcg
      541 acggaaggag cgtgtcaggt gcggttgcaa ctatcgatgt tgttggaggc catcaccatc
      601 cgagcccact tctgggcgat gtagttgacc tcgtttgtcg ccttggagca gctgaacatc
      661 ccgaaagact cgccccggta acgggataat ccttcggcag ccgtggccat cgcctcgtcc
      721 caactcacag gttgcagacg accgtccttg cggaccaatg gccgggtcag ccgcgcatag
      781 tcgcgtttca ccttcacctc ctttccttcg atatctccag ttgagagtac agactggcac
      841 ggctcggtca cttcaaaccc cctcctcacc ctcggcctcg gtcgagacat ccccggcaac
      901 ctggaggacg tggggcacac cgcggggggt gggagcgaac tcatgacgtc tcccagcgaa
      961 tcggtcggcg tcggcgccga tcctccgtac caacgcatag aagggcatcg ccgtcaacgc
     1021 gatggctcca cacgccacgt atcccgccgc aaagccctgg ccctccgcca cataaccaag
     1081 cccggtctgt ccggcgacac ttgccccacc ggtgatcatc gagtcgaacg agaccacggt
     1141 cgcacgctgt ctcgagggag caactctgtg aatgaacgcc tgtcgaaccg ggctctgggc
     1201 tccaaaggca acgccgctga cacacaagag aaccagggcg gcgacgaacg tcgaagccag
     1261 gccaacaccg atcagcgcga gggagaacac ggccgaggac cacagaaaga tggtgcttcg
     1321 tcgcccacac cagcgggaga cgatctccac gattccattc cccaacatca tcgacaatga
     1381 gagaagggcg gcaacgactc ctgccaccca caccacgtct cgctccagaa gctcgaggaa
     1441 ataaggctgc caggcgtacc acgcccaggc gaagacaccc atctgcgccg ccgtgccgag
     1501 catgaggaga cgcaaagatc gatcggccca gccgaaacga acgcccgccc gcccaatcgc
     1561 cattgcttcg gagggaagct ggctcaaggt cacccgccgt ggctcgaacc ccaggtcgtg
     1621 catccccacg aaggcgagaa cgaacagtgc gatgaggaga cccgagcgca cgagaaaggg
     1681 aatggcgagg tccacctggc cgagtatccc gcctcccacc gtgccgatga tcatcgctgc
     1741 tcggcttatc gcctgccctc gactgaagat ccgatccatc tcttctgtgt acccgagcga
     1801 atgcatgcca tcgaccagcc aggcctccat cgcccctgag tagaaagtga agccgagacc
     1861 gatcaacaaa gagatcgcga cgaaggcaac cacgctcccc ccgatctgtg ccagccccac
     1921 gtaggcgagc gtggtcagtg ccaggaccac gacggacagc aggaacgaag ctcgccggcc
     1981 gatggtgtcc gccaccacgc ctgtgggaat ctcgaacaag gtcgttccca acgaccaggc
     2041 ggcgttggcg atgaagacct cgctgatcgt gagacctgcg tcgaggagga acagtgtgtt
     2101 gacaccccag atcaccgagg aggcgagggt gtagagaccg gcgtagacgt agtagcggcg
     2161 caccagactg cggacggtct cggacatcat gcgaccatat atccggtctg gggaggcggc
     2221 gacgtaccca gccgacctca gccctcgggg tcgagatgcg gagcgacgtg agccagatga
     2281 ccgccgcgga tcacgagttg catcgcttgc ggatcggtca gcagcgacac gtcgtgaagg
     2341 gggtcccctt cgacgatcac caggtcggcg atcatcccgt cagcgatacg cccgatcagg
     2401 tcgtcgagac ccaacgcccg ggctcccgcc gcggtggccg ccaccagcgc gtcactcgtc
     2461 gtcatccccg catccaccat gacggccagc tcaccggcgg ccttctcctc gggtgtggaa
     2521 tcgaaaccca ttgcgatgtt gacaccggcc tcgatcgccg ccaccagcgt cgcctcggct
     2581 tgactcacgt tgtattcgcc ccgctccacg agtgcttccg accaggcttc gtgacgaccg
     2641 gtgacgacgt cgtggagaaa ggtgatcgtc ggcaccaggg tctgacccgt ctccgccata
     2701 cgggcgagat actccggtct ctgattcaga tagaacccgt gttctatggt gtcgacaccc
     2761 tcctcgatcg ccatgatggt gccctccaaa ccctcgcagt gggcggcgac ccggaagccc
     2821 tgccggtgga cttcgtcgac cagggccgcc aactcctcgc gcgtcacttg aggcggcgcc
     2881 gggttctcgt attcgaccga tcgcgccccc gtcatcatga tcttgaccag gtcggccccc
     2941 cgccggatct gctcacgcgc cgccttacgc atctcatcgg gcccgtcggc ctcccggtac
     3001 atgccttcga aatgacgtcc tcccggagag gtggcgctga cgattcgccc gcatatcaaa
     3061 agcctcggag agagaaaggc cccataacgc accgcttgcc ggatggcgag gatctcatca
     3121 ccccaggcac cgacgtctcg gaccgtggtg atgcccatgc ggagggcgcg cctcagaccg
     3181 gcggccacca ggtggccgtt cacctctggc ctgagcttct ccatgccctc cgtcatgctg
     3241 acctgtggct gcagggagag atgggtatga acgtcgatga gacccggcat cacgaatcgg
     3301 ccgtcgaggt cgatcgacgt cgtctcgccg gaggccgctt cggtctgaac tcgtccgttg
     3361 gcgatgaaca cgtcgccctc gaacgggtcc gagccgctgc catcgaagat gcgagcattc
     3421 cggagcgcat aggcatcctc gggtggggcc ggatcggcga ggagggctgg caagacgaat
     3481 gggacactca ccgttggcaa cctaccaacg ccgagatagt gtccgacact ccacgcgatg
     3541 aggagtgaga tttcgatggg ccaaattccc gcatcggcag agttcgtgat cgtgggcgcc
     3601 ggggtgcacg gtatgagcac ggcctggcac ctcgcgatgg agcgaggtag cggcgacggc
     3661 atcgtggtcc tcgacaagac ggctcctggt gcgggtgcca caggaatcgc ctgcgggtgc
     3721 gtccgcaact tctacatgac agagcccatc cacgctctca tcagggagtc ggtggacgtg
     3781 tggaactacg acccggtgct gttgggcttc cagcaggtcg gctacgtctc ctgcggtgag
     3841 gagaaccaga tctctgagta cgagaagatc cacaagagcc agaactcggt cggctacacg
     3901 tccgatctct acgtcggaca ggatgcgcgt gacttcctga cgtcggtctg gcccgacttc
     3961 agacaccaca gcatcgacgt ggtgatccac gagaagctgt ctgggtatgc gggcaccgcc
     4021 cag
//
LOCUS       DASUTA010000401         3204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1284195, whole genome shotgun sequence.
ACCESSION   DASUTA010000401 DASUTA010000000
VERSION     DASUTA010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284195"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFZ80_07915"
     CDS             complement(<1..416)
                     /locus_tag="VFZ80_07915"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX6287397.1"
                     /translation="MDDSLKEVYAPIAWAQMWAKTKDAAKTTYEIFKSAGKQYGIDRV
                     NRMAAAVAYRTMFALAPLLLVAVSVLGVFVGDVEARGEILETVTRVAGETVAEALEDF
                     LRSVSQSSSVAGIVGFVLLLWTSSSLFMELQNNLND"
     gene            404..1426
                     /locus_tag="VFZ80_07920"
     CDS             404..1426
                     /locus_tag="VFZ80_07920"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015746153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX6287398.1"
                     /translation="MSHPFPPPVATMEAKVSEKWPKGEFVYEPKWDGFRSLSWSAPEI
                     RIDSRNERPLLRYFPELTDALKQLPEGTVVDGEIVVVVDGATDFDTLQQRIHPAESRI
                     NMLSEQTPAELVAFDLLADRGEDLRERPFAERRERLVALADGLELPWHLTPQTDSEDV
                     ARAWFHEFEPAGCDGIIAKDPGLSYQHGKRAMIKIKHRRTIDVVVGGFREHKDGDKIG
                     SLLLGLYNDSGDFHFIGHCSGFPNADREELYRRFMELRADDSFDEQARAPGGQSRWSA
                     GKDNSWIPVQPGVVVEVSYDQLEGDRFRHASRFHRWRPDKDASQCTMDQLERPEGAAF
                     REVVGS"
     gene            1549..2463
                     /locus_tag="VFZ80_07925"
     CDS             1549..2463
                     /locus_tag="VFZ80_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6287399.1"
                     /translation="MGQDNVVTIKGLTHDYGEVVALRDLDLEIPPGVTGLVGANGAGK
                     TTMLRLLLGLLHPTRGSIEVMGHDPETQPLQVRAVMGYMPEGSCLPKDQTAADFVAYA
                     AQLAGIPPREARRRSSETLFLVGLEEERFRFLGDFSTGMQQRVKLAQAIVHDPDLLLL
                     DEPASGLDPAGRNQMLRLIRRLGEFDINVIVSSHVLTDIEETCSWVVMLDAGDLLRSG
                     PLEGFERHGTVQVEVLADAAGLAERLTKRGFTVSVDDRRLVVGPGDETIEAAVVEEAA
                     AVDSGLVRMVRGSASLEDLFVRPNGESP"
     gene            2460..>3204
                     /locus_tag="VFZ80_07930"
     CDS             2460..>3204
                     /locus_tag="VFZ80_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287400.1"
                     /translation="MSDQVIYDRGYRTYEGPRSGPRGARRAVYREGIRRVLGLGRKAR
                     LKIFPWALISIALVAAAVFIGVHWAIGNIEESLREGVPTYGGLFDFYSAISLLFIALA
                     GPQLLSPDRSSGALSVYFSRPLTVDGYLGAKVGAFATVVGAIYIVPQVVLHIGLALIS
                     DDGFLPYMGDNLDILWKVPVTTLAFVGLHGAIVFILASVIDRTGIAAAAFLGLIVAGS
                     AIAAAVGAAGFTGSRWISLLSIDQHPRIIR"
BASE COUNT          570 a         1032 c         1004 g          598 t
ORIGIN      
        1 atgtcgttca gattgttctg caactccatg aacaggctgg agctggtcca cagcagcagg
       61 acgaacccca cgatgcccgc cacactgctc gattggctga ccgatctgag gaaatcctcc
      121 agtgcttctg cgacggtttc accggcgaca cgggtcaccg tctcgaggat ttcaccacga
      181 gcttcgacgt cgcccacgaa cacaccgagc accgagaccg cgacgagcag cagtggcgct
      241 agggcgaaca tcgtgcgata ggcgacggcg gcggccatcc ggttcacccg gtcgatcccg
      301 tactgcttcc ccgccgactt gaagatctcg taggtggtct ttgccgcgtc cttggtcttg
      361 gcccacatct gcgcccaggc tatcggggca tacacttctt tcagtgagtc atccatttcc
      421 cccacctgtc gcgacgatgg aggccaaggt ctccgagaag tggcccaaag gagagttcgt
      481 atacgagccg aagtgggacg ggttcagatc tctctcgtgg tcggcaccgg agattcgaat
      541 cgactcccga aacgagcggc cgctactgcg gtatttccct gagctgaccg acgccttgaa
      601 acagctccca gagggaaccg ttgtcgacgg tgagatcgtg gtggtcgtcg acggcgccac
      661 cgacttcgac accctccagc aaaggattca tccagcggag tcccggatca acatgctgtc
      721 ggaacagacg cccgccgagt tggtggcgtt cgatcttctc gccgaccggg gagaagatct
      781 gagggagagg ccgttcgccg agcgtcgtga gcgtctcgtc gctctcgccg atgggctcga
      841 actcccctgg cacctgacac cgcagaccga ctcggaggac gtcgcccgcg cctggtttca
      901 cgagttcgag ccggcggggt gtgatgggat catcgccaag gatcccggcc tgtcatatca
      961 gcacgggaag agggcgatga tcaagataaa gcaccggcgg accatcgacg tcgtggtggg
     1021 cgggtttcgg gagcacaagg acggagacaa gatcggttcc ctgctactcg gcttgtacaa
     1081 cgactccggc gacttccact tcatcggtca ctgctcgggc ttcccgaacg ccgaccggga
     1141 ggagctctat cggcggttca tggagctccg agcggatgac agcttcgacg agcaggccag
     1201 ggcgccaggc ggacaaagcc gctggtccgc gggcaaagac aactcctgga taccagtgca
     1261 gccgggtgtg gtcgtcgaag tcagctacga ccagctcgag ggcgaccgct ttcgccatgc
     1321 ctcccgattc catcgctggc gaccggacaa ggacgccagt cagtgcacca tggaccagtt
     1381 ggagcgcccc gagggggccg cattccgcga ggtcgtcggc agctagagac cgtcctgtgt
     1441 cgattttgtg gtgtgtagga ccacaaaatc gacacagaac tgtctcgggg agaccttggg
     1501 ggcatccctg attcttcaag cccgggcgtc gtgccagact ggccgggaat gggtcaggac
     1561 aacgtcgtaa cgatcaaagg gctgacccac gactacggcg aggtcgtcgc cctgcgcgac
     1621 ctcgatctcg agatcccgcc cggggtcacc ggtctggtcg gcgccaacgg cgcaggaaaa
     1681 accacgatgt tgcgtctcct cctcggtctt ctccatccga ctcgtggctc gatcgaggtc
     1741 atgggccacg atcccgagac ccagccactc caagtgaggg cggtgatggg ctacatgccg
     1801 gaaggctcat gcctgcccaa ggaccagact gctgccgact tcgtcgccta cgcggcccaa
     1861 ctcgccggga taccgccccg cgaggcccgt cgccgctcct ccgaaacact cttcctcgtc
     1921 ggtctcgaag aggagaggtt ccgctttctc ggtgacttct cgacgggcat gcagcagcgg
     1981 gtgaagctgg cgcaggcaat cgtccacgac cccgatctcc tactcctgga tgagccggct
     2041 tccggtctcg acccggccgg gcgcaaccag atgcttcgtc tcattcgccg actcggtgag
     2101 ttcgacataa acgtcatcgt cagctcccac gtgctcaccg atatcgagga gacgtgctcc
     2161 tgggtcgtga tgctggacgc aggcgacctc ttgcgatcgg gcccactcga aggtttcgag
     2221 agacacggaa cggtgcaggt cgaggttctc gccgacgccg cgggtctggc cgagcgactc
     2281 accaagcggg gcttcaccgt ctcggtggac gaccgcaggc tggtggtcgg ccccggcgat
     2341 gagaccatcg aggcggccgt tgtcgaggag gccgccgcag tcgactcggg tctggtgcga
     2401 atggtgaggg ggtcggcgtc tctcgaagac ctgttcgtcc gtcccaacgg cgagagccca
     2461 tgagcgacca ggtcatctac gaccgcggtt accggaccta tgagggaccc cgcagcgggc
     2521 ccagaggagc ccgtcgcgcc gtctatcgag aggggatacg acgcgtcctc ggtctcggtc
     2581 gaaaggctcg cctcaagatc ttcccatggg cactgatctc gatcgccctg gttgcagcgg
     2641 ccgtgttcat cggggtccac tgggcgatcg gcaacatcga agagagcctg cgagaaggtg
     2701 tccccacata cggcgggctc ttcgacttct actcggcgat ctcgctgttg ttcatcgcgc
     2761 tcgccggacc gcagctgctg tcgcccgacc gctcgtcggg ggcactgtcg gtgtacttct
     2821 ctcgacccct gaccgttgat ggatatcttg gagcgaaggt tggagccttc gccactgtcg
     2881 taggcgcgat ctacatcgtc ccccaagtcg tcctccacat cggtctcgct ctcatctccg
     2941 acgacggctt cctcccctat atgggtgaca acctggacat cctctggaag gtcccggtca
     3001 ccaccctcgc cttcgtcggt ctccacgggg cgatcgtctt catcctggcg tcggtgatcg
     3061 atcgcaccgg catcgcggcg gcggcgttcc tcggcctgat agtggctggg agtgccattg
     3121 cggccgccgt gggagcagcc gggttcaccg ggtcgaggtg gatctccttg ctctcgatcg
     3181 accagcaccc gcggataatc cggg
//
LOCUS       DASUTA010000402         5687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1025544, whole genome shotgun sequence.
ACCESSION   DASUTA010000402 DASUTA010000000
VERSION     DASUTA010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025544"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VFZ80_07935"
     CDS             <1..495
                     /locus_tag="VFZ80_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6287401.1"
                     /translation="VVRMPEGLTAADSLPSRILVPVSATRSTRAAEEFAYSLAKASGG
                     RTFALHVVNRPEGQGMMLEDGAVEDSLTAGQEMVAEAATFGERLGVDVETGVRVAPNA
                     EEEIVRFGNSGAFDLLVIGASTRSLTNRPFYGHRVSYMLSQSELPVAVVSLPATVSPS
                     APSH"
     gene            536..3355
                     /gene="gcvP"
                     /locus_tag="VFZ80_07940"
     CDS             536..3355
                     /gene="gcvP"
                     /locus_tag="VFZ80_07940"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019609134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEX6287402.1"
                     /translation="MPNPAERFADRHIGPKPADIEKMLATVGVASMDDLIAETVPEAI
                     LDTALDLPAALSEPETLARLRELADANTVLPSLIGLGYHDTILPPVIQRNVLESPAWY
                     TAYTSYQPEISQGRLEALLNFQTMVSDLTGLEIANASLLDEPTAAAEAVAMARRLFKG
                     DREVFLVDTDCHPATRSVIETRAVAAGVEIRDLTDDADPSLAFGVLLQYPGSSGAIPD
                     HRQVAARFKEAGAIVAVATDLLALTLLSPPGEWGADIAVGSAQRFGVPLGYGGPHAGF
                     IATLESHVRALPGRLVGVSTDRQGRMAYRLALQTREQHIRREKATSNVCTAQVLLAVI
                     SGLYACWHGPDGLKAIATECNRLASVIASSFSAAGYEPSNESWFDTITVSVPGKAEEM
                     VSRALDAGINIGRDGGDLIRIAVDEKTDDDLVDRLLSALEIRRVETSARLPAGLGRES
                     AYMTHPVFSLHQSEHEMLRYLRRLADRDLALDRTMIPLGSCTMKLNATTEMVPITWEG
                     FSGVHPFAPVGQAAGYLELIDELESYLASITGYAAVSLQPNAGSQGELAGLLAIAAYH
                     RSRGDEQRDVCLIPASAHGTNAASAVMAGMRVVVVNTDSSGNIDVEDLEAKAGEHAEH
                     LAALMVTYPSTHGVFEAGIKDVTRIVHEHGGQVYVDGANLNALVGLARPADFGADIGH
                     LNLHKTFTIPHGGGGPGVGPVGARSHLAPFLPNHPQLPEAGPETGIGAVSGAPFGSAG
                     ILPIPYAYIRLMGVGGLKEATRVAILSANYVARRLDPYFPVLYTGSSGRVAHECIVDV
                     RPLTKSSGITAEDIAKRLADYGFHAPTLAFPVANTLMIEPTESESLEEIDRFCDAMIA
                     IRSEADRVASGEWPRDDNPLFNAPHTAEDVSSDVWERAYGREVAAYPLESLRRDKYWP
                     PVGRIDGVAGDRNLVCACPPLDAYT"
     gene            3438..4712
                     /locus_tag="VFZ80_07945"
     CDS             3438..4712
                     /locus_tag="VFZ80_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287403.1"
                     /translation="MRFKGELKFPDVDHPGVPVQFVIDGDQAELVVDDESLGRWSLYD
                     VHARRLIASAFQIDLDGTEVTFVASDPIDFAYRGVEHMAESWAAIKSKRLATRSLAIR
                     KSRRGTTPSRIDDLREAMESNLEAMEPGRLPPRPSGGLPGLGAADRSPARGEQPQIPV
                     AREPREEVVEARSEPVSSPSPSPSPALDAEREALAEERRRLEEERRQLEEQRRAAEER
                     EANLVEAYRLEMQRLDAEREELLRQAPGAISEESDGAAGQLDVASHSEVDELVAPEPV
                     VEPEPEPEPEQEPEPEPEPEPEPEPKPEPEPEPEPEPEPEPEPEPEPEPEPVSSGVVD
                     LEEYETRGQDDGLSGVAATLAPRPGPGPEPEPVLAGAAKERRGGIMGAVKAAFRSGPR
                     DHEHQFIEAPGGIGITRYVCEECGYVSISVGD"
     gene            4670..>5687
                     /locus_tag="VFZ80_07950"
     CDS             4670..>5687
                     /locus_tag="VFZ80_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX6287404.1"
                     /translation="MRGVWVRVDLGRRLTRSTRAQPDNEFRHLGRYARPVPPVRPVVL
                     SGGSGTRLWPLSTPDLPKQFRALIADRSLFGLTMARLYGLPDLAPAIVVTGERHVELV
                     QAEVESAPIDVGSILVEPVGRNTAPAVLAATMIANPDDVMLIVPSDHLIRDADRFRET
                     VSIALEVAGGGGIVTFGIEPTRPDTGFGYIEIGERDGSAFQVARFKEKPGLDEALEMI
                     RDGRHLWNSGMFVGRANQILAEAGKHCPQLLESLEATVPSVTGGVARLDDGFQSIEAI
                     SFDHAIMERTERARVVPMDVGWSDVGSYRSLLDVSELDESGNHIFGNVTHSGLTNSYV
                     SAETR"
BASE COUNT         1001 a         1857 c         1827 g         1002 t
ORIGIN      
        1 gtcgttcgca tgccagaggg gctgaccgcc gccgactcac ttcccagtcg catcctcgtc
       61 ccggtctcgg cgaccaggtc gaccagggcc gccgaggagt tcgcttattc actggccaag
      121 gcgagcggcg gcaggacctt cgccctccac gtcgtcaatc gaccagaagg ccaagggatg
      181 atgctcgagg acggtgcggt ggaagacagc ctcaccgcgg gccaggagat ggtcgccgag
      241 gcggccacgt tcggggagcg gctcggtgtg gacgtcgaga caggggttcg cgtcgccccg
      301 aacgctgagg aggagatcgt tcgatttggc aactcgggtg cgttcgacct gttggtcatc
      361 ggggcaagca cccgctcgct caccaaccgg cctttctacg gtcatcgtgt ctcgtacatg
      421 ctctcccaat ccgagcttcc cgtcgcggtc gtgagcctgc cggcaacggt gtcgccgtca
      481 gcaccgagtc actgaaggat cgcttcatcg tgcttaagat cggtggaaga gaccgatgcc
      541 gaacccagcc gagcgattcg ccgaccgcca cataggaccg aagccggccg acatcgagaa
      601 gatgttggcc accgtggggg tcgcctccat ggacgatctc atcgccgaga cggtccccga
      661 ggccattctc gatacggctc tcgacctccc ggccgctctc agtgagcccg agactctggc
      721 gcgtctccgg gagctcgccg acgcgaatac ggtccttccc tcgctcatcg gccttggtta
      781 tcacgacacg attctccctc cggtcatcca gcgcaacgtc ttggagagtc cagcctggta
      841 caccgcatac acctcgtatc agccggagat ctcccagggt cgtctcgagg ccttgctcaa
      901 tttccaaacg atggtctccg atctcaccgg tctcgagatc gccaacgcct cgttgctcga
      961 cgagccgacc gcggctgcgg aggctgtggc catggcgcgc cggctgttca agggagatcg
     1021 cgaagtcttt ctcgtggaca ccgactgcca tccggccacc cggtctgtga tcgaaacccg
     1081 cgccgtcgca gcaggtgtgg agattcgtga tctcaccgac gacgcagatc ccagccttgc
     1141 cttcggggtt ctcctccagt atccggggtc gtctggtgcg atcccggacc accgccaggt
     1201 cgctgcccga ttcaaggaag ccggtgccat cgtcgcggtg gcgacagatc ttctcgcgct
     1261 caccctcctc agccctcccg gggagtgggg cgccgacatc gcggtcggtt ccgcccagcg
     1321 tttcggcgtg cctctcggat acggcgggcc acatgccgga ttcatcgcca ccctcgagtc
     1381 tcacgttcga gcccttcctg gccgactggt cggggtctcc acggatcgtc agggtcgaat
     1441 ggcgtaccgt ctcgccctgc aaaccagaga gcaacacatc cgtcgcgaga aggcgacgtc
     1501 gaacgtgtgc accgctcagg tgctgcttgc ggtgatttca gggctctacg cctgttggca
     1561 cggtcccgac ggcctcaagg ccattgccac cgagtgcaat cgcctggcat cagtcatcgc
     1621 gagctccttt tccgcggcgg ggtacgagcc gagcaacgag tcctggttcg acaccatcac
     1681 cgtgtcggtc ccggggaagg ccgaagagat ggtctcccgg gccctcgacg caggcatcaa
     1741 catcggtcgt gacggcgggg acctcatccg gatcgctgtc gatgagaaga ccgacgacga
     1801 tctggtcgac cgactgctga gtgctctcga gattcggcgg gtcgagacgt cggcgaggct
     1861 ccccgccggg ttgggcaggg agtccgccta catgacgcat ccggtgttca gcctccacca
     1921 gtcagagcac gagatgctcc gctacctgcg ccgtctcgcc gaccgagacc ttgcattgga
     1981 tcggacgatg atcccgctgg gctcgtgcac gatgaagctc aacgccacca cagagatggt
     2041 cccgatcacg tgggagggct tttccggtgt ccacccattc gccccggtcg gccaggctgc
     2101 gggttatctc gagctgatcg atgagctgga gagctatctg gcctcgatca ccggctacgc
     2161 ggcagtgtcg ctacaaccaa acgccgggtc gcagggtgaa ctggcagggt tgctcgccat
     2221 agccgcctat cacaggtctc gtggtgatga gcaaagggat gtctgcctca tcccggcgtc
     2281 ggcgcatggg accaacgcag cctcggcggt catggcgggg atgcgcgtcg tcgtcgtgaa
     2341 caccgacagc tcgggcaaca tcgatgtcga ggacctcgag gccaaggcgg gcgaacacgc
     2401 cgaacacctc gccgccctga tggtgaccta cccgtccacc cacggtgtct tcgaggccgg
     2461 gatcaaggat gtcacacgaa tcgttcacga gcacggtggg caggtatatg tcgacggcgc
     2521 caacctcaac gcgctggtcg gtctggcaag gcccgccgac ttcggcgccg acatcgggca
     2581 tttgaacctg cacaagacgt tcacgattcc gcacggcggg ggagggccgg gggtaggtcc
     2641 ggtaggagct cgatcacacc tcgccccctt cctgcccaat catccccagc ttcccgaggc
     2701 cgggccggaa accggtatcg gcgcagtctc cggcgctccg ttcggctcgg cagggattct
     2761 ccccatccct tacgcataca ttcgcttgat gggcgtgggc gggctcaaag aggcgacccg
     2821 tgtcgccatc ctctccgcca actacgtcgc cagacgcctc gacccctact tccctgtctt
     2881 gtacaccggc agttcgggac gtgtcgccca cgagtgcatc gtcgatgtcc ggcccctcac
     2941 caagagctcc ggcatcaccg ccgaggacat agcgaagcgg ctcgccgact atggctttca
     3001 tgccccgaca ctcgccttcc cggtggcgaa cactctcatg atcgagccca ccgagtcgga
     3061 gagcctcgaa gagatcgatc ggttctgcga tgcgatgatc gccatcagat cggaagccga
     3121 ccgggtggct tccggtgagt ggccccgtga tgacaatccg ctgttcaacg ctccacacac
     3181 cgccgaggac gtgagctcgg acgtttggga acgcgcctac ggacgagagg tggcggcata
     3241 tccgctcgag agcctgcggc gggacaagta ctggccgcct gtgggccgca tcgatggcgt
     3301 ggccggcgat cgcaatctag tgtgcgcctg ccctcctctc gacgcctaca cctgatgcat
     3361 cttgacattt cgtgtaccaa tgggatcttt aaccctgtca gggacacctg tggcatcgag
     3421 gatttgaggt tttctgcgtg agattcaagg gtgagctcaa gttcccggat gtcgatcatc
     3481 cgggtgtgcc ggtccaattc gtgatcgatg gggaccaggc cgagctcgtg gtcgacgatg
     3541 agtcgttggg ccggtggtct ctctacgacg tccatgcccg ccgcttgatt gccagcgctt
     3601 tccagatcga tctcgacgga accgaggtga ccttcgtcgc cagcgacccc atcgacttcg
     3661 cctaccgcgg ggtcgagcac atggcagagt cgtgggcggc gatcaagtcg aagcgtctcg
     3721 ccacccgctc cctcgctatc cgcaagtcga ggaggggcac gactccgtcg aggatcgatg
     3781 acctgagaga ggccatggaa tcgaatctcg aggccatgga gcccggtcgt ctcccacccc
     3841 gtccatccgg tggtcttccg ggtctcggtg ctgccgatcg aagcccggca cggggcgagc
     3901 agccacagat tcccgtagcc agggagcctc gcgaagaggt tgtggaggca aggtccgagc
     3961 cggtttcctc gccatcgcca tcgccatcgc cggcgctcga cgccgagcgt gaggccctgg
     4021 ccgaggagcg gcgacgtctg gaggaggaac ggcgtcagct cgaagagcag cgacgggcgg
     4081 ccgaggagcg cgaggccaac ctcgtcgagg cgtaccgcct cgagatgcag cgcctggatg
     4141 ccgagagaga agagctcctg cgtcaggcgc ctggtgccat ctctgaagag tcggacggag
     4201 cggccggcca gctcgatgtc gcatctcact cggaggtcga cgagctggta gcacccgagc
     4261 cggtagtgga acccgaaccg gagccggagc cggagcagga gcctgagccg gagccggagc
     4321 ctgagccgga gccggaaccc aagcctgaac cagagcccga gccggagccc gagccggagc
     4381 cggagccgga gccggagccg gagccggagc ccgaaccggt ctccagtggt gtggtcgatc
     4441 tcgaggagta cgagaccagg ggccaagatg acggcttgtc gggagtggca gccaccctgg
     4501 cgccaaggcc cgggcccggg cccgagccgg aaccggtcct ggccggggcg gccaaggaac
     4561 gcaggggtgg gatcatgggt gccgtcaaag cggcattccg ctcgggccca cgcgatcacg
     4621 agcatcagtt catcgaggca ccagggggaa ttggcatcac ccggtacgta tgcgaggagt
     4681 gtgggtacgt gtcgatctcg gtcggcgact gactcgatcg acgcgagcgc aacctgacaa
     4741 tgagttccgc cacctcggcc ggtacgctcg cccggtgcct ccagtaagac cggtagtgtt
     4801 atccgggggg tccgggacca ggctctggcc gctctcgact cccgacctgc ccaagcagtt
     4861 ccgagccctg atcgcagatc gctctttgtt cggtctgacg atggcgaggc tctacggtct
     4921 ccccgacctc gctccggcca tcgtcgtcac cggcgagagg catgtcgagc tggttcaagc
     4981 agaggtcgaa tcggcgccga tcgatgtggg ctcgatcctc gtcgagccgg ttggccgcaa
     5041 cacagctcct gccgtcctcg ccgcgacgat gatcgccaac ccggacgatg tcatgttgat
     5101 cgttccctcc gatcatctca taagggacgc cgacagattc cgagagacgg tgtccatcgc
     5161 cctcgaggtt gcgggtgggg gcggaatcgt caccttcggc atcgagccca ccagacctga
     5221 tacaggcttc ggctacatcg agatcggaga acgggacggc tccgcattcc aggtggcgcg
     5281 gttcaaggag aaaccgggcc tcgacgaagc gctcgagatg atccgagacg gcaggcacct
     5341 ctggaactcg ggcatgttcg tcggccgcgc caatcagatc ctcgccgaag ccggcaagca
     5401 ctgtccgcag cttctcgaga gtctggaggc caccgtgccg tccgtgacgg gtggtgttgc
     5461 tcgcctcgac gacgggtttc agtccatcga ggcgatctca ttcgaccacg caatcatgga
     5521 gcgcaccgag cgggcacgtg tggtgcccat ggacgtcggg tggagcgatg tcgggtccta
     5581 tcgctcgttg ctcgacgtca gcgagctcga cgagtccggt aatcacatat tcggcaatgt
     5641 gacgcattcc ggcctgacca actcctatgt gagcgcggaa acacggc
//
LOCUS       DASUTA010000403         4504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_637694, whole genome shotgun sequence.
ACCESSION   DASUTA010000403 DASUTA010000000
VERSION     DASUTA010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637694"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1739)
                     /gene="leuS"
                     /locus_tag="VFZ80_07955"
     CDS             complement(<1..1739)
                     /gene="leuS"
                     /locus_tag="VFZ80_07955"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005458094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX6287405.1"
                     /translation="MSYDHETIEAKWQKRWEQERVFYVEEDDSKPKFYNVCMYPYPSG
                     AIHQGHVRNYTYGDLLTRYKRMKGFNVLSPMGWDSFGLNAENAAIRDGIHPRISTETN
                     IETMRSQIKELGSMYDWDRELASHHPSFYKWDQWLFIQMWKKGLAYKAEAPVNWCPKD
                     QTVLANEQVIDGECERCGTPVVRKNLAQWFFKITDYADRLLEDIEMLEHWPERVKTMQ
                     RNWIGRSEGARFTIEVEGGGSFDVFTTRPDTIFGMTFCVLAPEHPLVESLIEGSETEA
                     EARAYIVAAAQASEIERMAEGEKTGVFTGAYAINPANDRRVPIYIADYVLMGYGTGAI
                     MAVPGQDQRDWDFADKYGIDIIRTVQPPADFDGEAYIGDGETINSDFLDGLDMARAKE
                     RINSWLEDEGIGERTVQYRLRDWLISRQRYWGCPIPMISCEECGMVPVPEDDLPVLLP
                     DIEDYLPKGRSPLAADEDFVHTACPECDGPAQRETDTMDTFVDSSWYFYRYADAHNSE
                     AMFDPAKPAYWMPLDQYIGGIEHAILHLLYARFITKMLYDMGMSSVEEPFARLFTQGM
                     ITLDGAKMSKSKG"
     gene            1804..2658
                     /locus_tag="VFZ80_07960"
     CDS             1804..2658
                     /locus_tag="VFZ80_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX6287406.1"
                     /translation="MTIERVGVIGGGLMGSGIAEVCARAGSSVVVHEIDAETAEAAME
                     RIEGSMARAVDRGKMSESERASILGGIEVSTDLGAQADRQLVIEAATENEDLKRRIFS
                     QLDEVVTDSDAILASNTSSIPITRLAAATSRPEKVVGLHFFNPVPVMALVELVTTVKT
                     AVDVRERARDYVAGLGKTVIHAKDRAGFVVNMLLVPFMLAAIRMYEAGVASAEDIDTG
                     MRLGANHPMGPLELTDFVGLDTTKYVADVLFEEFKEPLYASPPLLTRMVEAGLHGRKT
                     GEGFYKYG"
     gene            complement(2664..3044)
                     /gene="mscL"
                     /locus_tag="VFZ80_07965"
     CDS             complement(2664..3044)
                     /gene="mscL"
                     /locus_tag="VFZ80_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX6287407.1"
                     /translation="MVQEFRDFIAKGNVVMLAVGFIMGVAFQGVVNSLVENVIMPIVA
                     IPFGEPNFDSLSATINGSEILYGSFVTAVVVFLLTALAVFLFIVKPYNAFAERRSKED
                     EPEEAGPTEIELLTEIRDSLARRP"
     gene            complement(3064..4056)
                     /locus_tag="VFZ80_07970"
     CDS             complement(3064..4056)
                     /locus_tag="VFZ80_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX6287408.1"
                     /translation="MRTESVRIIGDTDDMDAAERLLKLLGLLEGRIDWTAEELAARLD
                     VTTRTIRRDITRLRALGYPVEAMAGPGGGYRLGAGGKLPPLLLDDEEALAVALGLRVS
                     ATSAVGGLEDASLSAMAKLEHVLPPRLRNRLEDVSEATTSTLVGARTEVDHSSLSVVA
                     TTIRKQERMRCRYVDSEGRVTERHIEPTRLVHTGRRWYLVAFDLDRDDWRTFRLDRVS
                     EPSPTGMRAKTRRGPDPVELVQRGITVDAWKFPASVILDMSAGHAERLISPTVGTLEP
                     IDDATCRLVIGADEMAWLARYLLSLPVRFDVEEPEELRAELASIGAELVNQFAY"
     gene            4077..4289
                     /locus_tag="VFZ80_07975"
     CDS             4077..4289
                     /locus_tag="VFZ80_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287409.1"
                     /translation="MEEREEGKTMLSLFTYEDARRLTDERRRRALAVYEARRTTTNDS
                     FVRPERTGAEVIELSFGTACPDSIGA"
BASE COUNT          873 a         1367 c         1383 g          881 t
ORIGIN      
        1 ttccctttcg acttcgacat cttcgccccg tcgagagtga tcattccctg ggtgaacagc
       61 cgggcgaacg gctcctcgac gctcgacatt cccatgtcgt agagcatctt cgtgatgaaa
      121 cgtgcgtaga gcagatggag aatggcgtgc tcgatgccac cgatgtattg atccagcggc
      181 atccagtagg caggctttgc tggatcgaac atggcctcgg agttgtgggc gtctgcgtat
      241 cgatagaagt accaggatga gtcgacgaac gtatccatcg tgtcggtctc ccgttgcgcc
      301 ggcccgtcgc attcgggaca ggcggtgtgg acgaaatcct cgtcggcggc cagaggcgac
      361 cgacccttgg gcagatagtc ctcgatgtcc ggaagaagca ccgggagatc gtcctcgggg
      421 accgggacca tcccgcactc ctcacagctg atcatcggga tcgggcagcc ccaatagcgc
      481 tgtcgagaga tcagccaatc gcgcaatcgg tattgcacgg tcctttcacc gatgccctca
      541 tcctccaacc aggagttgat cctctccttg gcccgggcca tgtcgagccc gtccagaaag
      601 tcggaattga tggtctcgcc gtcccctatg tacgcctcac cgtcgaagtc ggccgggggc
      661 tggacggtgc gaatgatgtc gatgccgtac ttgtcggcga agtcccagtc acgctgatcc
      721 tgtccgggta cggccatgat cgccccggtg ccatagccca tgagcacata gtcggcgatg
      781 tagatgggga cccggcgatc gttggcgggg tttatcgcat aggcaccggt gaagacaccg
      841 gtcttctccc cttccgccat ccgctcgatc tccgatgcct gcgccgcagc aacgatgtag
      901 gcacgagcct cggcttcggt ctcacttcct tcgatcaagg attcgaccag cggatgttct
      961 ggtgcgagca cgcagaaagt catgccaaag atggtgtcgg gtcgtgtcgt gaagacgtcg
     1021 aaggagccgc cgccctccac ctcgatcgtg aatcgggcac cctcggatcg gccgatccag
     1081 ttgcgctgca ttgtcttcac ccgctccggc cagtgctcca acatctctat gtcctcgagc
     1141 aaccggtcgg cgtaatcggt gatcttgaag aaccattggg ccaggttctt ccggactacg
     1201 ggcgtcccac aacgctcaca ctccccatcg atcacctgct cgttcgccag caccgtctga
     1261 tccttggggc accaattgac tggagcttct gccttgtagg ccaggccctt cttccacatc
     1321 tggatgaaca gccactggtc ccacttgtag aaactggggt gatgtgaagc aagctcccga
     1381 tcccagtcgt acatcgatcc cagttccttg atctgagacc gcatggtctc gatgttggtc
     1441 tcggtgctga tccgggggtg gatgccgtcg cggatggccg cgttctcggc attcagacca
     1501 aaactgtccc atcccatcgg tgacaggacg ttgaagccct tcatcctctt gtatctggtc
     1561 agcaggtcgc cataggtgta gttgcgaaca tgaccctggt ggatcgctcc cgaggggtac
     1621 gggtacatgc agacgttgta gaacttcggt ttggagtcgt cctcttcgac atagaagacg
     1681 cgttcttgtt cccaacgctt ctgccacttg gcttcgatgg tttcgtggtc gtatgacatc
     1741 tagggcggtc agggtaccta ttgctgccaa gatgctcaca cccttaaacg aggagaaacc
     1801 tcaatgacaa tcgaacgtgt tggcgtaatc ggaggcggcc tcatgggctc gggcatcgcc
     1861 gaggtctgcg ctcgagcggg cagctcggtg gtggttcatg agatcgacgc cgagacggca
     1921 gaggccgcga tggaaaggat cgaagggtcc atggctcggg cggtcgatag agggaagatg
     1981 agcgaatctg agcgtgcgtc catcctggga ggcatcgaag tatctaccga cctcggcgcc
     2041 caggcagacc gtcagctcgt gatcgaagcc gcaaccgaga acgaggacct gaaacgaagg
     2101 atcttctccc agttggacga agtcgtcaca gacagcgacg ccatcctggc ctccaacacc
     2161 tcctcgatac ccatcacacg gcttgctgcc gcaacctctc gcccggagaa ggtggtcggg
     2221 ctccatttct tcaacccggt cccggtcatg gcgctggtgg agctggtcac cactgtcaag
     2281 accgcagtgg atgtacggga gcgggcccgg gactacgtcg ccgggctggg caagacggtg
     2341 atccacgcca aagatcgggc agggttcgtg gtcaacatgc tcttggtccc cttcatgctg
     2401 gcggcgatcc ggatgtatga ggccggtgtg gcctcggcgg aggacatcga cactggcatg
     2461 aggcttggag ccaaccatcc gatgggccct ctggagttga ccgacttcgt cggcctggac
     2521 accaccaagt acgtcgccga cgtcctgttc gaagagttca aggagcccct gtacgcgtct
     2581 ccacccctgc tgacccggat ggtggaggcc ggcttacacg gcagaaagac cggcgaaggc
     2641 ttctacaagt acgggtgatc agctcaggga cggcgggcga ggctgtctct gatctcggtg
     2701 agtagttcga tctcggttgg accggcttct tcaggctcgt cttccttgct gcgacgctcg
     2761 gcaaaggcgt tgtacggctt gacgatgaat agaaacaccg ccaaggcggt gagcaggaag
     2821 accactaccg ccgtgacgaa agacccatag aggatctcgg agccgttgat cgtcgccgac
     2881 agtgagtcga agttgggctc accgaacggg atggcgacga tcggcatgat cacattctct
     2941 accagggagt tgacgacacc ctggaaggcg acacccatga tgaagccgac ggccaacatg
     3001 acgacgttgc ccttggcgat gaagtctctg aactcttgaa ccatctggcg agactaccgc
     3061 tggtcaataa gcgaactggt tgaccagctc ggccccgatc gaggcgagct cggcacgcag
     3121 ctcctcgggc tcctcgacat cgaatcgcac cgggagactg aggagatagc gagccagcca
     3181 ggccatctcg tcggcgccga tgacgagacg acaggtggcg tcgtcgatcg gctcgagggt
     3241 tcccaccgtc ggcgagatga gtctctcggc gtgaccagcc gacatgtcca agatcaccga
     3301 cgccgggaat ttccaggcgt cgactgtgat gccacgttgc accaactcga ccgggtctgg
     3361 ccctcgtcgg gttttggccc tcatccccgt cggcgagggc tcggagaccc tgtcgagtcg
     3421 aaaagtgcgc caatcgtctc gatcgaggtc gaaggcgacg aggtaccagc ggcggccggt
     3481 gtgaaccagc cgcgttggct cgatgtggcg ctctgtgacc ctgccctcgg agtcgacata
     3541 tcgacagcgc atacgttctt gcttgcgaat ggtggtcgcc acgaccgaga gcgaggagtg
     3601 atcgacctcg gtccgggcac ccaccagcgt cgaggtagtc gcctccgaga cgtcctccag
     3661 gcggttgcgg agtcgagggg gcagcacatg ctccagcttt gccatcgccg atagcgaggc
     3721 gtcttccaga ccgccgacgg cgctggtggc ggagacccgg agaccgagtg ccacggcaag
     3781 ggcctcctcg tcatccagga gcagaggcgg caacttgccg cctgctccga gccgatagcc
     3841 gccgccagga ccggccatgg cctcgaccgg gtagccaagg gctcggagac gggtgatatc
     3901 ccgtctgatg gtgcgggtcg taacatcgag acgcgccgcc aactcctctg cggtccagtc
     3961 gatacggcct tcgagaagcc ccaggagctt cagcaaacgc tccgctgcat ccatgtcatc
     4021 agtgtctccg attatgcgga ccgattctgt ccgcattctc tcatagcctg gtctgtgtgg
     4081 aggaaagaga agagggaaag acgatgctca gcctgttcac ctatgaggac gccagacgtc
     4141 tcaccgatga gcgccgccgc cgggcgctgg ccgtttatga ggcacggaga acgacgacga
     4201 acgacagttt cgtccgccct gagagaaccg gcgccgaagt catcgagctc agcttcggga
     4261 ctgcctgccc cgattccatc ggagcctgag atggtgtgga gccatacggt cactacggac
     4321 gctggtgaga tggtctgtgc gcccttgacc cccgatctct tccccgacct cgaaacggtc
     4381 ttcggtgacc gcggtgttgc ccgccgatgc ttctgcatgc attggagaag acccgatggc
     4441 ggctatgagg acgcacgcga caaccgggat cgattcgccg atatcaccaa cgacgggcca
     4501 cctc
//
LOCUS       DASUTA010000404         2568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1544271, whole genome shotgun sequence.
ACCESSION   DASUTA010000404 DASUTA010000000
VERSION     DASUTA010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544271"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VFZ80_07980"
     CDS             <1..708
                     /locus_tag="VFZ80_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX6287410.1"
                     /translation="SSAPAQNWGDGARFSTNSRAPTIGDWDGDGLDDLAYQGICGGSG
                     KAHQCWRVHSSRGDSFTAPEDWGATPARQVKSMPVDLDGDGRDDLLFQSECEDESCWF
                     VQLSDGKSFGEPMRAGRVSSAESRAFRFFDFDGNGTDDVISWATGDDESRIEARFGSA
                     TGLSKVVVLARIDGPIDEVHLERAGGHAPARASVRLPCGDDSCVETLFATSSRSLVDG
                     DHYRSAMLGRRGVPIIT"
     gene            complement(701..1498)
                     /locus_tag="VFZ80_07985"
     CDS             complement(701..1498)
                     /locus_tag="VFZ80_07985"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX6287411.1"
                     /translation="MADLRFGSFLAPNMTPVYRAIADAVGERLGMSTELIVEEDYESC
                     RNDVNDICFVCSLPYVTFEREGIAPAEPIAAPVLQGERYQGRPIYFSDVVVHRDSDAR
                     SFLDLRGRTWCFNEPLSQSGYGVTRHHLVSLGETGGFFSEVVQSGFHEESIRWVAEGV
                     VDASAIDSQVLAVEMRNRPELFDEIKVIDAIGPSTIQPLAVSKRFDEEFREEVRSVLV
                     AFHETERGREILDHGLIERWVPVDASSYDDIRRMLEACEAAGFMEIR"
     gene            complement(1575..2522)
                     /locus_tag="VFZ80_07990"
     CDS             complement(1575..2522)
                     /locus_tag="VFZ80_07990"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6287412.1"
                     /translation="MKRMLLVLAVMGLLVAACGDDAGGGTEEITLIVNPWTASRLNAE
                     VAANIIESELGNPVEITEVNENDAMFTGMADGTLDAVLEIWPSGVTDAETAYFDDGSV
                     VNIGELGAVGKIGWFVPQYVIDEHPELATWEGFQDPALAALFATPETGENGRFLGTDP
                     SYSQYDEQIIENLGLPLEVVFSGSEPATVAEVDARVAAQEPILLYWWTPTAAVSAYDL
                     VNVTLPEYTEECGASAAAGDGGVDCDYPEDVLFKAASGMLEEKDPAVFEFLQNFTITT
                     EDQLEMLPSVEIDGNDPAAVAADWVGANEDIWSAWLPQS"
BASE COUNT          469 a          835 c          796 g          468 t
ORIGIN      
        1 agctccgcac ccgcccagaa ctggggtgac ggagcccggt tctcaacgaa ctcccgggct
       61 ccgacgatcg gcgattggga cggcgatggt ctcgacgatc tcgcctacca ggggatctgc
      121 gggggctccg ggaaggccca tcaatgctgg agggtccatt cgtcgcgggg cgactcgttc
      181 acggcacccg aagactgggg tgccaccccg gcgagacagg tcaagtcgat gccggtcgac
      241 ctcgatggcg atggtcgtga cgacctgctc ttccagtcgg agtgtgagga tgagagctgt
      301 tggttcgtcc agctctccga cgggaagtcc ttcggcgagc cgatgcgagc cggccgggtc
      361 tcatccgcag agtcgagagc cttccggttc ttcgacttcg atggaaacgg aaccgacgac
      421 gtgatctcct gggcgacggg tgacgacgaa tcgcgaatcg aggcgcgatt cgggtcggcc
      481 acgggcctct cgaaagtcgt cgtcctcgct cgaatcgacg gcccgatcga tgaggtccat
      541 ctggagaggg ccggggggca tgcacccgcg cgagcctcgg tgcgtctccc ttgtggcgat
      601 gacagctgcg tagagacact gtttgccacc tcgagccggt ccttggtcga cggagatcac
      661 taccgaagcg ccatgctggg gcgccgcggg gttcccatca tcacctgatc tccatgaaac
      721 cggctgcctc acaagcctcg agcatccttc ggatgtcgtc gtagctggag gcgtcgaccg
      781 gcacccacct ctcgatcagc ccatggtcga ggatctctct gcccctctct gtctcgtgga
      841 aagcgacgag aacactgcgg acttcctccc ggaattcctc atcgaagcgc ttggagactg
      901 caaggggctg gatcgtggac ggaccgatcg catcgatcac cttgatctca tcgaagagct
      961 ctgggcgatt ccgcatctcc accgccagga cctgtgagtc gatagccgag gcgtcgacca
     1021 cgccttcggc tacccaacga atcgactctt cgtggaaccc ggattgaacc acctcgctga
     1081 agaacccccc tgtctccccc agcgagacca ggtgatgccg ggtgactcca tatccggact
     1141 gcgacaacgg ctcgttgaag caccaggtgc ggccgcgcag gtcgaggaac gatcgggcat
     1201 cgctgtctcg gtgaacgacg acatccgaga agtagatggg acgaccctga taacgctcac
     1261 cttggaggac aggggcggcg atcggctcgg cgggagcgat cccctcacgt tcgaaggtca
     1321 catagggcag gctgcagacg aagcagatgt cgttgacgtc gttgcgacag ctctcgtagt
     1381 cctcttcgac gatcagctcc gtggacatcc cgagacgctc tccgaccgcg tccgcgatcg
     1441 cccggtacac cggcgtcatg ttcggggcga gaaacgagcc aaaacgcaag tcagccatct
     1501 caggccaccc taaaccttcg cagagaagtc agccccgggg cgaacccggg gctgaccttc
     1561 ggttcgttgt tccctcagct ctgaggcagc caggcgctcc agatgtcctc gttggcgccc
     1621 acccagtccg cggccaccgc ggccgggtcg ttcccatcga tctcgacgga gggcagcatc
     1681 tcgagctgat cctctgtggt gatcgtgaag ttctggagga actcgaacac cgcggggtcc
     1741 ttctcctcga gcattcccga ggcggccttg aagaggacat cctccgggta gtcgcagtcg
     1801 acaccgccat caccggcggc ggcgctggcg ccacactcct cggtgtactc cggcagggtc
     1861 acgttgacca ggtcgtatgc cgaaacggca gcggtcgggg tccaccagta gaggaggatc
     1921 ggctcttggg ccgcgacccg ggcatctacc tcggccaccg ttgccggctc cgagccggag
     1981 aagaccacct cgagaggaag accgaggttc tcgatgatct gctcgtcgta ctgggagtac
     2041 gaggggtcgg ttccgaggaa tctgccgttc tcaccagtct ccggtgtggc gaacagggcg
     2101 gccagagccg gatcctggaa tccttcccag gtcgccagct cggggtgctc gtcgatcacg
     2161 tactgaggca cgaaccaccc gatcttccct acggcgccga gctcaccgat gttcaccacc
     2221 gaaccgtcgt cgaagtacgc ggtctcggcg tcggtgacgc ccgaaggcca gatctcgaga
     2281 acggcatcga gggtgccgtc ggccatgcca gtgaacatgg catcgttctc gttcacctcg
     2341 gtgatctcca ccgggttgcc caactctgac tcgatgatgt tcgccgccac ttcggcgttg
     2401 agacgggacg cggtccacgg gttgacgatc agagtgatct cttccgtgcc gccaccagcg
     2461 tcgtcaccac aagcggcgac gagcagcccc atcaccgcca ggacaagcag catccttttc
     2521 atgcgtttct ccctaattcc ttgattcgag gggtcattcc ccgcccag
//
LOCUS       DASUTA010000405         5000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_766857, whole genome shotgun sequence.
ACCESSION   DASUTA010000405 DASUTA010000000
VERSION     DASUTA010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766857"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VFZ80_07995"
     CDS             complement(<1..461)
                     /locus_tag="VFZ80_07995"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEX6287413.1"
                     /translation="MRTAIAGLVSLTLLLAACSPAEEATESTTTSEPTTTSSTTTTTT
                     STTTSTTVEEGPPSLLNGLPVDDPELIERRVLAVKIDNHPLANPHSGIEQADMVIEVN
                     VEGITRFITLWHESDVDYLGPNRSGRPTDAALLAALNEPTFTISGAQAWVQ"
     gene            complement(527..931)
                     /locus_tag="VFZ80_08000"
     CDS             complement(527..931)
                     /locus_tag="VFZ80_08000"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX6287414.1"
                     /translation="MTEFVVSMENQPGRLAALTEALAAFGVNIEALTAYGHNGDGTVR
                     LITSDGPTTRRVLDEAALHHEEHTVLTVQLPHRPGELARITRLLADAGVNIDALYVLR
                     ANSDGIELAISVDQPESAVPHLEVTGGVLAEQ"
     gene            complement(945..1946)
                     /locus_tag="VFZ80_08005"
     CDS             complement(945..1946)
                     /locus_tag="VFZ80_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEX6287415.1"
                     /translation="MRRLPVACDSLYRFRVSDHTPERPMSMKRFRVAGHSMLPTLQPG
                     QEVVAVNTRTPRPGNLVVFQHPNDTSLWLIKRLVDDEGTVLSDNREDSAHDSHDFGPI
                     HLSRLWNVVDRLDADTFVLACEMLISEDPDLAELVDRWGIPEFWNRRPGFETLVLLIL
                     EQQVSLASGAAMYGRVARLTGRVTPGNVLAAGEHRLRSIGLTRQKTGYLLDLARRLVD
                     GELDLAILEELSEDVARRELISIRGIGPWTADAYLLSASRRPDMWPVGDRALQVGSAE
                     VLGLDSVPDEAELEILAESWRPVRAVAARLIWHSYLSERGRVEPPDATLVHAPETRA"
     gene            1925..2332
                     /gene="sodN"
                     /locus_tag="VFZ80_08010"
     CDS             1925..2332
                     /gene="sodN"
                     /locus_tag="VFZ80_08010"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HEX6287416.1"
                     /translation="MRQVIVAHAHCDLYCGVYDPAQAKIEALSVLKIAEKYQDSDDEV
                     FRGRALQLKEERAELVKHHLMVLWADFFTADHRKEFENLDDLFWRAIHQAGEAKKSAD
                     PKDGQKLIDLIDEIAEIFWKTEKAKTMGVYPPS"
     gene            complement(2329..2550)
                     /locus_tag="VFZ80_08015"
     CDS             complement(2329..2550)
                     /locus_tag="VFZ80_08015"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEX6287417.1"
                     /translation="MKRLARIACGYGLIGAGTVMIFIPGPGLITIVGGLALLQDDVKW
                     AGKAASWLKQKAGRSEPYRDSDPDSGSST"
     gene            complement(2625..4262)
                     /gene="metG"
                     /locus_tag="VFZ80_08020"
     CDS             complement(2625..4262)
                     /gene="metG"
                     /locus_tag="VFZ80_08020"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006538383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX6287418.1"
                     /translation="MTRRILVAVAWPYASGPPHLGQFGGAYLPPDIFARYHRLIGNEV
                     LMVSGSDANGTPITVKAEELGITPKEVVDTYHPEILSYWDSLGIEFDLFTTTMTENHH
                     RVTQDFFLRLLENGYMIKDTSLQFYDPEAERFLPDRYVEGECPHCGYPDARGDQCDNC
                     GRTLDPTDLINPRSRLSGATPERRETEHYFLDLPAFKERLQEWLESKTEWRRHVRNWA
                     LGMVEDIPVRAMTRDIDWGVDVPVDDLGPGKKIYVWFDAVIGYLSAAIEWASYTDDPD
                     AWEKWWKDPEAESWYFIGKDNIPFHAVIWPAMLMGHGNLNLPTEIPANQFITFGGSKA
                     SKSKGVGYSLAWYIERLQPDAIRYAIGSVLPEQHDTDISDEEIIRRVNEELVATWGNL
                     VNRVLSMSDRNFDGRVPMPGELTPDDHTILELGRETLDTVAAHIEKADLRAGLRAGMD
                     AAGGVNAYLNATEPWNVLKKDRERGATILWTAIQAIAAIRVALYPYLPHSAVTIGEML
                     GTGPDVISWSAPAVPADTMLGEITPVFVKLEPDALDA"
     gene            complement(4370..>5000)
                     /locus_tag="VFZ80_08025"
     CDS             complement(4370..>5000)
                     /locus_tag="VFZ80_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6287419.1"
                     /translation="GGLLVALIPLATIAAAHFLVPGERFQIRTLPGLLLALAGSALLV
                     GLGGTSIEGVGNLWRGVLFMMIGVLMAGIGGALSRRFALELSSNELVLPQFTVNTLVV
                     SIVLPLFFGFDVGTVDTASWLLIAGIGVLGTTLSFGSFLVAAGLNPASRLALVSYAIP
                     VLSVILAVIFLGEEVTLAIVAGASLILAGVVLAERSTVHVPEPGVATAR"
BASE COUNT          923 a         1497 c         1670 g          910 t
ORIGIN      
        1 ttctggaccc aggcctgcgc ccccgagatg gtgaaggtcg gctcgttgag agccgccagc
       61 aatgcggcgt ctgtgggacg gccggagcgg ttgggaccga ggtagtccac gtcgctctcg
      121 tgccagaggg tgatgaaacg ggtgattccc tccacgttga cctcgatgac catgtcggcc
      181 tgctcgatcc cggagtgggg gttggcaaga gggtggttgt cgatcttgac ggcgaggacg
      241 cgtcgttcga tcagctcggg atcgtcgacc gggaggccgt tcaagagtga cggaggccct
      301 tcctcgacgg tcgtcgatgt ggtggtagag gttgttgtgg tggtggtgga actggtggtg
      361 gtgggttctg aagtggtcgt cgactcggtg gcttcctcgg ctgggctgca cgcagcgagg
      421 aggagagtga gcgagaccaa cccggcaatt gcggtccgca ttgacgcgat gttacgaagc
      481 cgctctcggc tacctggaat tgccgggaag agatctgaga ccggggttac tgctcagcga
      541 ggacgccgcc ggtcacctcg aggtggggga ctgcggattc aggctgatcg acagagatgg
      601 ccaactcgat cccgtcggag ttggcgcgca gcacgtagag cgcatcgatg ttcactccgg
      661 cgtcggcgag cagtctggtg atgcgggcca gctcaccggg ccggtgcggg agttgcaccg
      721 tcaacacggt gtgctcctcg tgatgcaagg cggcctcatc gaggacacgg cgagtcgtcg
      781 ggccgtcgct ggtgatgaga cggaccgtgc cgtcgccgtt gtgcccgtaa gcggtgaggg
      841 cttcgatgtt gacgccgaac gcggcgagag cctcggtgag ggcggcgagc cgcccgggct
      901 ggttctccat gctcactacg aactcggtca tcacagagac gactctacgc gcgcgtttcg
      961 ggtgcgtgca cgagagttgc gtcaggtggc tcgacccggc ctctctcgga cagatacgag
     1021 tgccagatca gccgggcggc aacggcccga acgggacgcc atgactcggc cagtatctcc
     1081 aactcggcct cgtcaggtac cgaatccagc cccaagacct cggcggagcc gacctggagt
     1141 gcccggtcgc ccaccggcca catgtcgggc cgtctcgatg ccgagagcag gtaggcgtcg
     1201 gcggtccacg ggccgatgcc cctgatcgag atgagctcgc gacgggccac gtcctcggag
     1261 agctcctcga ggatcgcgag gtcgagctca ccgtccacga ggcgccgggc caggtcgagc
     1321 aggtagcccg tcttctgtcg ggtcaagccg atcgatctga ggcgatgctc gccggcggcg
     1381 aggacgttgc ctggggtcac ccgaccggtg agtcgtgcca cccggccata catggccgca
     1441 cctgaggcga gggagacctg ttgctcgagg atcaggagca ccagagtctc gaaaccgggc
     1501 cttcggttcc agaactccgg tatgccccac cggtccacca gctcggcaag gtcggggtcc
     1561 tcggagatga gcatctcgca ggccaggacg aaagtgtcgg cgtcgagcct gtcgacgaca
     1621 ttccacagac gggacaggtg gatagggccg aagtcgtgag agtcgtgtgc gctgtcttcc
     1681 ctgttgtcgg agaggaccgt tccctcatcg tcgacgagtc tcttgatcag ccagagcgag
     1741 gtgtcgttcg ggtgctggaa cacgacgagg tttcctggcc ggggtgtgcg ggtattgacc
     1801 gctacgacct cctgaccggg ctggagcgtg gggagcatcg aatgtccggc cacccggaag
     1861 cgcttcatcg acataggtct ctcaggagta tggtcggaca cacgaaacct atataaggag
     1921 tcacatgcga caggtaatcg tcgcacatgc ccactgcgac ctctattgcg gcgtctacga
     1981 cccggcgcag gccaagatcg aggcgctctc tgtcctcaag atcgccgaga agtaccagga
     2041 ctcggacgac gaagtctttc gcggtcgagc cttgcagttg aaagaggagc gagccgagct
     2101 cgtcaagcat catctgatgg tgttgtgggc cgacttcttc acggccgacc atcgcaaaga
     2161 gttcgagaat ctcgacgacc tgttctggcg ggcgatccat caggcaggtg aggccaagaa
     2221 gtcggccgat ccgaaggatg gccagaagct gatcgacctg atcgatgaga tcgccgagat
     2281 cttctggaag accgagaagg ccaagacgat gggggtctac ccgccgtcct aggtgcttga
     2341 cccggagtca gggtcggagt ctcgatacgg ctccgaccga cccgctttct gtttgagcca
     2401 gctggccgcc ttgcctgccc acttaacgtc gtcttgaagt agcgccagcc cgccgacgat
     2461 cgtgatcagc cctggcccgg gtatgaagat catcacggtg ccggcaccga tcaggccgta
     2521 gccacaggca atgcgcgcca aacgcttcat gtcataaagc tactcgtctc tgcgagttgc
     2581 ggaatcgtga ccttccgagt ccgtctcacc gagctcgaaa caggtcaggc gtccagggca
     2641 tccggctcca gcttcacgaa gaccggtgtg atctctccca gcatcgtgtc ggcgggcaca
     2701 gcgggggcag accacgagat gacatccggg cccgtgccca gcatctcccc aatggtgacc
     2761 gccgaatggg ggaggtaggg gtagagggcg accctgatgg cagcgatggc ctgtatcgcc
     2821 gtccagagga tggtggcacc ccgctcgcgg tccttcttca agacgttcca cggctcggtc
     2881 gcgttcaggt aggcgttcac ccctccggcc gcatccatgc cggcgcgaag gccagcccga
     2941 agatcggcct tctcgatgtg agcggccacc gtgtccaagg tctctcgtcc cagttcgagg
     3001 atggtgtggt cgtcgggggt gagctcgccg ggcatgggaa ccctgccatc gaagttgcga
     3061 tcgctcatcg agagtactct gttgaccagg ttgccccagg tggccaccaa ctcctcgttg
     3121 accctgcgaa tgatctcttc gtctgagatg tccgtgtcgt gttgttcggg cagcaccgag
     3181 cctatggcgt atcgaattgc gtcgggctgg aggcgttcga tgtaccaggc gagcgagtaa
     3241 ccgaccccct tcgatttgga tgccttggag ccgccgaacg tgatgaactg attggccggg
     3301 atctcggtgg ggaggttgag gttgccgtgt cccatcagca tggccggcca gatcacggcg
     3361 tgaaagggga tgttgtcctt gccgatgaag taccacgact ccgcctcggg atccttccac
     3421 catttctccc aggcatcggg gtcgtctgtg taggacgccc attcgatggc agccgagaga
     3481 tagccgatga cggcatcgaa ccagacgtag atcttcttcc ccggtcccag gtcgtcgacg
     3541 gggacgtcga caccccagtc gatgtctcgg gtcatggccc ggaccggtat gtcctccacc
     3601 attccgaggg cccaatttcg aacatggcgc cgccactccg tcttgctctc cagccattcc
     3661 tgtagccgct ccttgaaagc cgggaggtcg aggaagtagt gctcggtctc cctccgttcc
     3721 ggagttgctc cgctcagcct cgagcgggga ttgatcagat cggttgggtc cagagtccgc
     3781 ccgcagttgt cgcattgatc gccgcgggca tccgggtaac cgcagtgcgg gcactcaccc
     3841 tccacatatc ggtccggtag gaatcgctcg gcctccgggt cgtagaactg cagcgacgtg
     3901 tccttgatca tgtacccgtt ctcgaggagc cggaggaaga agtcctgggt cacccgatgg
     3961 tggttctcgg tcatggtggt ggtgaagaga tcgaactcga tcccgaggga gtcccagtag
     4021 ctcaggatct cggggtgata ggtgtccacg acctctttgg gtgtgatccc caactcttcg
     4081 gccttcaccg tgatcggggt gccgttggcg tcggaccccg acaccatcag tacttcgttg
     4141 ccgatcaacc ggtggtagcg ggcgaagatg tccggaggta gataggcgcc gccgaactgc
     4201 ccgagatggg gtggcccgga tgcatatggc caggcaacgg cgacgagaat gcgacgtgtc
     4261 atcggtggcg cagggtagga gcttcgtccc cgaaactctc gagtgttcga tgaacatgag
     4321 ccgattcccg ccgtatacgg cgtgacgaac ccgcggttcg cgatgcccgc tatctggctg
     4381 tcgccactcc cggctcgggg acgtggaccg tggaacgctc cgccaggacg acaccggcga
     4441 ggatcagaga cgctccggcg acgatggcca aggtcacctc ttctccgagg aagatcacag
     4501 cgaggatcac ggagagcacc ggaatggcat agctgaccag ggcgagacgt gacgccgggt
     4561 tgagaccggc cgccaccagg aacgagccga acgacagcgt ggtccccaac acgccgatgc
     4621 cggcgatgag caaccagctc gcggtgtcca ccgtcccgac atcgaacccg aagaagagcg
     4681 ggagcacgat ggagaccacg agggtgttga ccgtgaactg aggaagcacg agctcgttgc
     4741 tcgacagctc gagggcgaat ctcctgctga gggcgccacc gataccggcc atgaggactc
     4801 cgatcatcat gaagagcacc ccgcgccaga gattcccgac tccctcgatc gaagtgcctc
     4861 cgagcccgac gagcaaagcc gacccggcca gagcgaggag tagcccgggc aacgtccgta
     4921 tctggaaccg ctcaccgggg acgaggaagt gggcggcggc gatcgttgcc aatgggatga
     4981 gggcaaccaa caggccgccg
//
LOCUS       DASUTA010000406         4458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_380869, whole genome shotgun sequence.
ACCESSION   DASUTA010000406 DASUTA010000000
VERSION     DASUTA010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380869"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..896)
                     /locus_tag="VFZ80_08030"
     CDS             complement(207..896)
                     /locus_tag="VFZ80_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HEX6287420.1"
                     /translation="MSEEELDLSTLSDDDLVAQMHDDLYDGLADEIDEGTRILLDRGW
                     DSSRVLNEALVEAMRIVGIDFRDGILFVPEVLLAAKAMKAGMAVLRPLLAETGAQTIG
                     TVVIGTVKGDIHDIGKNLVAMMLEGAGFEVFDLGINTDADGFINALDEHKPDILGMSA
                     LLTTTMPYMKVVIDTMVEKGIRDDYIVLVGGAPLNEDFGRAVGADAYCRDAAVAAETA
                     KALVGARRGAA"
     gene            1055..3553
                     /locus_tag="VFZ80_08035"
     CDS             1055..3553
                     /locus_tag="VFZ80_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019812063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6287421.1"
                     /translation="MSDLPTSANVVVIGAGIVGNSVVHHLAELGWRDLVQVDKGPLPN
                     PGGSTGHASNFIFPVDHNKEMTHLTRDSQQQYEAMGVQTTSGGIEVARTEARMEELRR
                     RMTSAKTWGIESHLLSPAEVKEKVPYIDESVILGGFFTPSVSVVDSLQAGTLMRQSAM
                     DKGALTVSPNTEVLDIETENGRVTAVVTDRGRIEAEYVVVACGVWSPRIARMAGATIP
                     LTPAVHQMIDVGPLEILQATGNEIGYPIVRDMDTFCYERQTAGSMEVGSYAHRPIFHH
                     PDTIPSIEEARLSPTELPLTQDDFDPQLEQAIELMPFLGDAEMRYGINGLLSLTPDAM
                     PLVGETEVRNLWSCAAIWIKEGPGTGRALAEWMTQGYPEVDPHASDIARFYEYQRTEA
                     HVLARCAEHFNKTYGIVHPREQWDSERNLRISPFHPATQALGAVYFEAGGWERPHWYE
                     SNADLVEKYGIEDRPHEWDARWWSPIQNAEHLAMREGVGMIDVTAFAIFDSTGPGVVD
                     YIEKMTVNKCDVPVGRAVYTPLLTPEGGFKADLTVMRLGDEHYRIISGGFDGPRDQHW
                     FTKHLPADGSVHFEDKTSAICTVGVWGPRARDLMARVTDDDLSDEALPYGHTKSVHIG
                     SIPARLFRISYVGEFGYEVYTSMQHGLALWNRLWDAGQDLGVVPVGAGVYGTTGRLEK
                     GYRLMGAELESEYNPVEAGLDRPKVKSADFIGKAAYLKAREEEPAAILCTLTMESHES
                     SEGTKRYPSGGNEPILTNGGERIVDAKGRVSRVTSAGAGPSLGKYLLMAYLPPEHAVE
                     GNELQVMYMNETYPVKVAVAGSTPLFDPDNDRMK"
     gene            3667..4407
                     /locus_tag="VFZ80_08040"
     CDS             3667..4407
                     /locus_tag="VFZ80_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005578109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein beta subunit/FixA
                     family protein"
                     /protein_id="HEX6287422.1"
                     /translation="MKRVPAPGAKIVLNEDARSIDTNLLSFTVSPHEECAVEEGVRIA
                     EAGEGSVTVLTLGPPESEEQLRAALAVGAHEAVLIPTDGEDWDPISTASAIADAIGEL
                     ETSNGRFDLILFGNESADSGGYQVGIRVAHALSRPVVAGIKGIDLTDEGVDLRRETTT
                     GSEVYRLPLPAIACVKEGINLPRYPTLKGRLNAKKVDVQSIERERVGSGQAMVRLKTP
                     VQEVSETTILEGPAAVVDLLEELQVVVK"
BASE COUNT          820 a         1440 c         1428 g          770 t
ORIGIN      
        1 agtcaccata ggccacgaag acaaggtcgt actcgccctt cgcccggcgg atccgctctt
       61 ccacggcgcc ggggatgagc tggggagtgt tgtgatagcg agcggggagg caggtgacgt
      121 cgatggcatc gagtcggttt cgggccacta cttccagcag ctcgcgcgcc atcgccccgc
      181 agccgacgac cagcacccgg gggccgtcag gcggcaccgc ggcgcgctcc caccaacgcc
      241 ttggcggtct cggccgccac cgccgcatcc cggcagtagg cgtccgctcc caccgcccgg
      301 ccgaagtcct cgttgagggg ggcgccaccc accaggacga tgtagtcgtc gcggatcccc
      361 ttctccacca tggtgtcgat gaccaccttc atatagggca tcgtggtggt gagcagagcc
      421 gacatcccga ggatgtcggg cttgtgctcg tcgagggcgt tgatgaaccc gtcggcgtcg
      481 gtgttgatgc ccagatcgaa cacttcgaag ccggctcctt cgagcatcat ggccaccagg
      541 ttcttgccaa tgtcgtggat gtcgcccttg accgtgccga tgacgacggt accgatggtc
      601 tgggctccgg tctcggcgag caggggacgg agcacggcca tccccgcctt catcgccttg
      661 gcggccagga ggacctccgg gacgaagagg atgccgtccc tgaagtcgat tcccacgatg
      721 cgcatcgcct cgacgagggc ctcgttcagg accctggagg aatcccaccc acgatccagc
      781 aagatgcggg tcccctcatc gatctcgtcg gcgagaccgt catagaggtc gtcgtgcatc
      841 tgggcgacga ggtcatcgtc ggaaagggtg gaaagatcca gctcttcttc gctcatccgt
      901 ccttctcccg cccgtgccac tctgtgggac gagtactgta cagtgggatt cgaaccgcaa
      961 tactatcggg gaaatcgcaa tgtggaaagc agctttcccc gtcgcggggt gcggtccact
     1021 ggacggaatg gcgtagacag agaagtgagg agctgtgagc gacctaccca cctcagccaa
     1081 tgttgtcgtg atcggagccg ggatcgtggg taactcggtg gtccaccatc tcgccgagct
     1141 gggctggcgt gatctggttc aggtcgacaa gggccctctc cccaacccgg gcggctcgac
     1201 cggccacgcc tccaacttca tcttccccgt cgaccacaac aaggagatga cccatctcac
     1261 ccgtgacagc cagcagcagt atgaagcgat gggtgtgcag acgacgagcg gcggcatcga
     1321 ggtggcgcgc acggaggcgc gaatggagga gttgcgtcgt cgcatgacct ctgcgaagac
     1381 ctggggaatc gagtcgcatc tcttgagccc ggccgaggtc aaggagaagg tcccgtacat
     1441 cgacgagtcc gtgatcctcg gcggcttctt cacgccgtcg gtctcggtgg tcgactctct
     1501 ccaggcgggc accctgatgc ggcagagcgc catggacaag ggcgctctca ccgtgtcacc
     1561 caacaccgag gtcctcgaca tcgagaccga gaacggcagg gtgaccgcag tggtcacgga
     1621 ccggggtcgg atcgaagccg aatatgtggt ggtggcctgc ggtgtgtgga gtccgcgcat
     1681 cgctcgcatg gccggcgcca ccatcccgct caccccggcg gtacatcaga tgatcgatgt
     1741 ggggcccctg gagatccttc aggccacggg aaacgagatc gggtatccca tcgtgcgcga
     1801 catggacacc ttctgctacg agcgtcagac tgcagggtcg atggaggtcg gttcctacgc
     1861 ccatcgtccg atcttccatc atcccgacac catcccctcg atcgaggagg cgcgtctgtc
     1921 tcccacggag ctccccctca ctcaggacga cttcgaccct cagctggagc aggccatcga
     1981 gttgatgccg ttcctgggcg atgccgagat gcgctacggg atcaacggcc tcctgtcgct
     2041 gacccccgat gccatgccgt tggtcggtga gaccgaagtg cgcaatctgt ggagttgtgc
     2101 cgcgatctgg atcaaggaag gccccggcac cggtcgggcc ctggcggaat ggatgactca
     2161 gggctatccg gaagtcgatc cccacgcttc cgacatcgcc cgcttctacg agtatcagcg
     2221 cacggaggcc catgtcctcg ccagatgtgc cgagcacttc aacaagacct acgggatcgt
     2281 ccaccctcga gagcagtggg actcggagcg caatctgcgc atcagcccgt tccaccccgc
     2341 gactcaggcc ctgggcgccg tctatttcga ggccggtggc tgggagcgtc cccattggta
     2401 cgagtcgaat gccgacctcg tcgagaagta cggcatcgag gaccggcccc acgaatggga
     2461 cgcccgctgg tggtcgccga tccagaatgc cgagcatctc gccatgcgcg agggcgtagg
     2521 gatgatcgat gtcactgcgt tcgccatctt cgattccacc ggacctggcg tcgtcgacta
     2581 catcgagaag atgacggtga acaagtgcga cgtgcccgtt ggccgggcgg tgtacacgcc
     2641 cctgctgaca ccggagggag gcttcaaggc cgacctgacg gtgatgcgtc tcggcgacga
     2701 gcactatcgc atcatcagtg gcggcttcga tggcccccgc gaccagcatt ggttcaccaa
     2761 gcacctcccc gcagatggct cggtccactt cgaggacaag acgtctgcca tctgcaccgt
     2821 cggtgtctgg ggaccccgtg cccgtgatct catggcgcga gtgaccgacg acgacctctc
     2881 cgacgaggcg cttccctacg gtcacaccaa gtccgttcac atcggctcga tcccggcccg
     2941 gctcttccgc atctcctatg tcggggagtt cggttacgag gtctacacca gcatgcagca
     3001 cggtctcgca ctctggaacc gtctctggga tgccggccag gacctcgggg tggtccccgt
     3061 gggtgccggc gtgtacggaa cgacgggtcg tctcgagaag ggctaccgcc tgatgggcgc
     3121 cgagctcgag tccgagtaca acccagtcga ggcgggtctc gaccggccca aggtcaagag
     3181 cgccgacttc atcggcaaag cggcctattt gaaggcccgc gaggaggagc ccgccgcaat
     3241 cctgtgcact ctgacgatgg agagccacga gtcgtcggag gggacgaagc gctacccctc
     3301 cggtggcaac gagcccatcc tcaccaacgg gggggagagg atcgtcgatg ccaaaggaag
     3361 agtctcgcgg gtgacgagcg ccggtgccgg cccctcgttg ggcaagtacc tgctcatggc
     3421 ctacctgcct ccggagcacg ccgtcgaggg caacgaactc caggtgatgt acatgaacga
     3481 gacctatccg gtgaaagtcg ccgtcgccgg cagcaccccc ctgttcgatc ccgacaacga
     3541 ccgaatgaag tagaggaaga gcccgatgcc gaacgctccc atctttctgg gcggattttt
     3601 gctcggaatc cggacaagaa accgcccaga acgggttgag gctcgggcat gagggtcctc
     3661 gtatgtgtga agagggttcc ggcgccgggg gccaagatcg ttctcaacga ggacgcccgc
     3721 tccatcgaca ccaacctgtt gtccttcacg gtcagccctc acgaagagtg tgctgtcgag
     3781 gagggggtgc ggatcgccga ggccggcgag ggaagcgtca cggtcctcac gttgggcccg
     3841 ccggagtccg aggagcagct gcgtgccgcg ttggcggtgg gagcccatga agcggtgctg
     3901 atcccgaccg acggcgagga ctgggacccc atctccacgg catcggccat cgccgatgcc
     3961 atcggtgagc tcgagacatc caacgggcgg ttcgacctga tcttgttcgg caacgagtcg
     4021 gcggactccg ggggttatca ggtggggatc cgggtcgccc atgctctgag ccgccccgtc
     4081 gtcgccggca tcaaaggcat cgacctcacc gacgaaggag tcgaccttcg acgtgagacc
     4141 accaccggct ccgaggtcta ccggctgcca ttacccgcca tcgcctgtgt caaggagggg
     4201 atcaacctgc cccgctatcc cactctgaaa ggacgcctca acgccaagaa ggtcgacgtg
     4261 cagagcatcg aacgagagcg tgtcggcagt ggccaggcca tggttcgcct gaagaccccg
     4321 gttcaggagg tctccgagac cacgatcctc gagggcccgg ccgccgtggt cgatctcctc
     4381 gaagagctcc aggtcgtggt caagtgatcc tcgtcctggc gacacatgac ggtggggcaa
     4441 tcgaggaaac cgcccttc
//
LOCUS       DASUTA010000407         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_895718, whole genome shotgun sequence.
ACCESSION   DASUTA010000407 DASUTA010000000
VERSION     DASUTA010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895718"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            531..737
                     /locus_tag="VFZ80_08045"
     CDS             531..737
                     /locus_tag="VFZ80_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287423.1"
                     /translation="MIELASAKDPGFDIGVFHSALGSMSRHRRPIFQMSDDEYEALLG
                     LVSRWGSELRRSLGRDGPGLDIGP"
     gene            complement(953..1714)
                     /locus_tag="VFZ80_08050"
     CDS             complement(953..1714)
                     /locus_tag="VFZ80_08050"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX6287424.1"
                     /translation="MDEVFSAINDPNRRHLLDQLFECDGQTLGELCAHFPEMTRYGVM
                     NHLRVLEAAGLVTTEKVGRSKFHYLNPVPIRLIQDRWISRFAEPRVGGITGIKALAET
                     GDLAVQKPKHIYRVYINATAEEVWEAMTDPDKTSLYFYGTRVESDWEVGSAINYRYPD
                     GSLASDGEILAIDVPTRLERTFRPLWDADLEAEGPAREVWALREVNGMVELSVEYYEI
                     GQKSLDNFTQPLGLAYIVSGLKSLVETGRSLPSPG"
     gene            1908..2060
                     /locus_tag="VFZ80_08055"
     CDS             1908..2060
                     /locus_tag="VFZ80_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287425.1"
                     /translation="MEGTEADIIFYVDLALPVDVWDEMVLPAHVREAWQSTVDPQRRQ
                     RRPSST"
BASE COUNT          377 a          646 c          635 g          425 t
ORIGIN      
        1 cggtggatgt ctgggacgag atggtgcttc ccgcccacgt ccgagaggcg tggcagagca
       61 ccgtagaccc gcaacgccgc caacgccgac cctcttcgac ataggggggc caaggctgtc
      121 cgagacctcc ccggctcgga aggattcgcc cttgctggcg gtgcaggcct ggtggtacag
      181 ggggtgacga gtcctgttac gaacgatctc gactacttcg ctgagcagcc aagtgaggtg
      241 gaccggttca tccccgttct cagagcatca cttcaagaca tgggcctgac agtcgtcacg
      301 gttgaagccg ctcccggctt cgtgcgcctg gtcgtggggg acggacagga cgaaacgctc
      361 gtagatctcg cctacgacac ccgcctgttc gctccggtcg acacagactt cgtcgagttc
      421 tggctctgga cgaactcgcc gccgacaaga cgcttgcggt cttcggacga gccgaaccga
      481 gagacttcgt cgacctattc gccctgtctc aacactattc cctcgatcgg ttgattgagc
      541 tggcgtcggc caaggacccc ggcttcgaca tcggcgtgtt ccacagtgcc ctcggttcga
      601 tgagccgcca ccgccggccg atcttccaga tgtccgacga cgagtacgag gcgttattgg
      661 gtcttgtctc acggtgggga tccgaactgc gccgttctct ggggcgggac ggtcctgggc
      721 tcgacatcgg tccctgaacc ccacgccgag catgttccac cactcaccgt gctccatgtt
      781 cgaggctggt ttgatggcac ttcgagtatc agcggggcgt gccggtgggc aatagttcag
      841 ttcggtctgc cgccgtggtg caacgcccgg atccggaacg ctcggaatcg gcggaacctc
      901 gcagcggttc gcgccacccg ctgatgaggg tttagccgtc tcacagggcg tgtcagccgg
      961 gagagggaag cgaccgcccc gtctcgacca ggctcttgag gcccgagacg atgtaggcca
     1021 ggcctagtgg ttgtgtgaag ttatccaggg atttctggcc gatttcgtag tactcgacgc
     1081 tgagctccac catcccgttt acctctctga gggcccagac ctctcgtgcc gggccttcgg
     1141 cttcgaggtc cgcatcccag agaggccgaa aggtcctctc caaccgtgtg ggaacatcga
     1201 tggcaaggat ctcaccatcg gaggcgagac tgccgtcagg ataccggtag ttgattgccg
     1261 acccgacctc ccagtctgat tcgacccgcg tgccgtagaa gtagagggag gtcttgtctg
     1321 ggtccgtcat cgcctcccac acctcctctg ccgtggcatt gatgtacacc cggtagatat
     1381 gtttcggttt ctgcactgcg aggtctcctg tctctgcgag agccttgatg ccggtgatcc
     1441 ctccgaccct gggctcggcg aatctgctga tccatcgatc ctggatcagt cgtatcggga
     1501 cggggttcag gtagtggaac tttgatcggc ccactttctc ggtggtgacc agcccggctg
     1561 cctcgagaac ccgcaggtgg ttcatgaccc cataccgggt catctccggg aaatgagcgc
     1621 acaactcacc gagggtctgc ccgtcacact caaagagttg atcaaggaga tgacgacggt
     1681 tcgggtcgtt gatggcgctg aacacctcgt ccatgggaaa cacagtatat gtgagtattt
     1741 actcacatat caacagtggc tttcggagac cctgcccgag atgagaggta aacccaggac
     1801 ccccagaccc cgctcgtttc cgaagcgcca gattggctcg cagatcaggt cgcggttacg
     1861 acctgactga tcggaatgat cgccctcttc tgtacgagcg ggtgctggtg gaagggaccg
     1921 aagccgacat catcttctac gtcgatctgg ccctcccggt ggatgtctgg gacgagatgg
     1981 tgcttcccgc ccacgtccga gaggcgtggc agagcaccgt agacccgcaa cgccgccaac
     2041 gccgaccctc ttcgacatag gggggccaag gctgtccgag acc
//
LOCUS       DASUTA010000408         2876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_896002, whole genome shotgun sequence.
ACCESSION   DASUTA010000408 DASUTA010000000
VERSION     DASUTA010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896002"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFZ80_08060"
     CDS             <1..185
                     /locus_tag="VFZ80_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX6287426.1"
                     /translation="DIDYALCMYCGICVEVCPFDALFWSPEYEYSEAHMGDMMHDMDR
                     LQDWLKTVPEPPALEK"
     gene            289..840
                     /locus_tag="VFZ80_08065"
     CDS             289..840
                     /locus_tag="VFZ80_08065"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX6287427.1"
                     /translation="MTFGSWIAEPVNWVFIAIGLVVLFAGFRVVTAKNVVHAALFLVA
                     ALAGTAGLFLLLSAEFVAWVLVLVYIGAVIVLFLFGIMITRAPTGLDEGLDSDRKAWP
                     ALLSLAVFVVMAWTSLEAFGSNEVVTTGSPTSTELLGESLLGRFVVPFEVVSFVLLAA
                     LIGGITIARRDLTPLEEEERAAV"
     gene            977..1282
                     /gene="nuoK"
                     /locus_tag="VFZ80_08070"
     CDS             977..1282
                     /gene="nuoK"
                     /locus_tag="VFZ80_08070"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009343500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX6287428.1"
                     /translation="MIVNQFLVLAAALFSMGVYGLLVRRNIVLILLSVELMLNAVNIN
                     LIAFESMLNTPQAIGQVFSIFVITVAAAEVGIGLAIVLMIFRNRRSANVDELNLMRW"
     gene            1293..>2876
                     /locus_tag="VFZ80_08075"
     CDS             1293..>2876
                     /locus_tag="VFZ80_08075"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX6287429.1"
                     /translation="MINDLLYLAAEEGEHVIEAGTGGFWGDTAWIMLVSPFVAFLAIL
                     AFGKRLKNQGGEIAVGMLAINLVWATALFILNMTEGVLDNSHTFEIARIGSGLVFELG
                     WALDGLSIMMYFLVNFVGLLVFIYALGYMRGDVRVPWFFAAFSMFAGAMLVLVGAPNM
                     IQLIIGWEGVGLASYFLIGFYWDDLENVRAGNKAFMTNKVADIGLFLGVIILGVTLGS
                     FDFAALDLAAIEQNEALAGVAVAAGLLLFIGAMGKSAQVPFHIWLPDAMAGPTPVSAL
                     MHAATMVTAGVFLMARTFPLFQNLAQEIRPWMVGIGAITLFAIGLLALVANDIKKVLA
                     YSTVSQLGYMMTAVAAGGYTAGLFHLFTHAFFKALLFLGAGSVIHAVHSNNMTDMGGL
                     RKYMPTTFWTFIIGSAALAGIFPLAGFWSKDEILATLDYEGYVLVMWIAIAGAFVTAF
                     YMTRCIALTFFGDYKGHGQPHESPRIMTVPLIGLAIPSVLFGLVNIPGIEWPGIQNFT
                     TWLGVRVVAMGDHHAEGIDI"
BASE COUNT          454 a          937 c          854 g          631 t
ORIGIN      
        1 tcgacatcga ctacgcactg tgcatgtatt gcggaatctg cgtcgaggtg tgtcctttcg
       61 acgctctctt ctggagtccc gagtacgagt actccgaggc gcacatgggc gacatgatgc
      121 acgacatgga ccgactccag gactggttga agaccgtccc cgaacccccg gcgctcgaaa
      181 aatgactcgg tcgcttcgct ccccagtcgt attcggtaac tggcattcga ttcttggatc
      241 tgacttcttc cggtcacctg gtgtctcgca cctcgaggag gcaatcccgt gacgtttggt
      301 tcctggatcg ccgagccggt caactgggtc ttcatcgcca tcggcctggt cgttctcttc
      361 gccgggtttc gtgtcgtgac cgccaagaac gtggttcacg ccgccttgtt cctggtggcc
      421 gcgctcgccg ggacggcagg gctcttccta ttgctctcgg ccgagttcgt ggcctgggtc
      481 ctggtgttgg tctacatagg tgcggtgatc gtgctcttct tgttcgggat catgatcacc
      541 cgcgccccca ccggtctcga cgagggtctc gactcggatc gaaaagcctg gccggcgctg
      601 ctctccctgg ctgtcttcgt ggtgatggcc tggaccagcc tcgaggcttt cggctccaac
      661 gaggtggtga ccacgggctc gccgacttcg accgaacttc tcggtgagtc gctcctcggt
      721 cgtttcgtgg ttcccttcga agtggtgtcg ttcgtgctgc tcgctgccct catcgggggg
      781 atcacgatcg cccgtcgtga cctgaccccg ctcgaggaag aggaaagggc agcggtgtga
      841 acaccacttg caagcgagcg cagcgagcaa cggccgaccg gcgtctgttt gcccgagccg
      901 gtgtcgacgc gctgccgttg tgggacgaac cagcccaagc cggggcgagg gcaaggtcgg
      961 ccggcaggag atcggcatga tcgtcaatca gttcctcgtc ctcgccgcgg ccctgttctc
     1021 gatgggtgtc tacggtcttc tggttcgacg caacatcgtg ctgatcctgc tctcggtcga
     1081 gttgatgctc aatgcggtca acatcaacct catcgccttc gagtcgatgc tgaacacacc
     1141 gcaggcgatc gggcaggtgt tctcgatctt cgtgatcacg gtggccgcgg ccgaggtggg
     1201 cattggcctc gccatcgtcc tgatgatctt ccgcaatcgc cgctcggcca acgtcgacga
     1261 gctcaacctg atgcgctggt gaggggcagg caatgatcaa cgacctcctg tatctcgctg
     1321 ccgaagaagg cgagcacgtc atcgaggccg ggaccggcgg gttctggggt gacaccgcat
     1381 ggatcatgct ggtcagccca ttcgtcgcct tcctcgccat cctcgccttc ggcaagagac
     1441 tcaagaatca gggcggcgag atcgcggtgg gcatgctcgc aatcaacctg gtctgggcca
     1501 ccgcgctgtt catcctcaac atgaccgagg gcgtgctcga caactcgcac accttcgaga
     1561 tcgcccgaat cggaagcggt ctcgtgttcg agctcggttg ggcactggac ggcctctcca
     1621 taatgatgta cttcctggtc aacttcgtcg gcctgttggt gttcatctac gccctcggct
     1681 acatgcgcgg cgacgtcagg gtgccatggt tcttcgcagc cttctcgatg ttcgcaggag
     1741 cgatgctggt gctcgttggt gcccccaaca tgatccagct gatcatcgga tgggagggtg
     1801 tgggtctggc cagctacttc ctgatcggct tctactggga cgacctcgag aacgtcaggg
     1861 cgggcaacaa ggcgttcatg accaacaagg tggccgacat cggcctgttc ctcggggtca
     1921 tcatcctcgg tgtcaccttg gggagcttcg acttcgcagc tctcgatctc gccgccatcg
     1981 agcagaacga ggcgctggcc ggcgtcgcgg tggcagccgg gctcctcctc ttcatcggcg
     2041 ccatgggcaa gagtgcccag gtgccatttc acatctggct ccccgacgcc atggccggcc
     2101 ccaccccggt gtccgccctg atgcacgcag cgacgatggt cacggccggg gtctttctca
     2161 tggcccgcac cttcccgctg ttccagaatc tggctcagga gatcagaccg tggatggtcg
     2221 gcatcggcgc cataactctg ttcgccatcg gcctgctggc cctggtcgcc aacgacatca
     2281 agaaggtcct cgcctactcg accgtgtccc aactcgggta catgatgact gcggtcgccg
     2341 ccggcgggta caccgcgggc ctcttccacc tattcaccca tgccttcttc aaggccctcc
     2401 tgttcctcgg cgccggctcg gtcatccatg ccgtccattc caacaacatg accgatatgg
     2461 ggggcttgcg taagtacatg cccaccacct tctggacctt catcatcggc tccgcggccc
     2521 tggccggcat cttcccgctg gcaggcttct ggtcgaagga cgagatcctc gccacgctcg
     2581 actatgaggg ttacgtcctg gtcatgtgga ttgccatcgc tggcgccttc gtcactgcct
     2641 tctacatgac ccgttgcatc gccctcacct tcttcggtga ctacaagggc cacggccaac
     2701 cccacgagtc accccggatc atgacggtcc ccttgatcgg cctcgccatc ccctcggtgc
     2761 tgttcggtct ggtcaacatc cccggtatcg agtggcccgg catccagaac ttcacgacct
     2821 ggctgggtgt gcgtgtcgtc gccatgggag atcaccacgc cgagggaatc gacatc
//
LOCUS       DASUTA010000409         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1805808, whole genome shotgun sequence.
ACCESSION   DASUTA010000409 DASUTA010000000
VERSION     DASUTA010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1805808"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..623
                     /locus_tag="VFZ80_08080"
     CDS             240..623
                     /locus_tag="VFZ80_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287430.1"
                     /translation="MFDTSNPAAPSEVSQITIPETADEDFAIGFGDLTVHEVEVPRGD
                     PNEGGPGLDDDKVAYFSWYAGGFRVVDITDPSNPEELGHYIDENGNNFWGVALAEDEN
                     GDRIVLGSDRDFGLFIFRYTGTIPS"
     gene            784..1320
                     /locus_tag="VFZ80_08085"
     CDS             784..1320
                     /locus_tag="VFZ80_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287431.1"
                     /translation="MCAPRVRWLLCVTGTAALLMMTALPAAADPPLHQRFTFSGAVTD
                     EGQACGEPLSWEFTGEVLLSRFIDTEGNLVRIQVQVREAGSVTDLATGEVIELPRTAF
                     TERVLFVEDGSIIVEDVGLSVRVTGPEDFLLDVGRFVVRFNPDELLQSSGQHPIREIN
                     PFSVEDPALLGAFCELFD"
BASE COUNT          342 a          644 c          692 g          339 t
ORIGIN      
        1 ggctcgtcgt cgcccgcagc cgacgtagcc gacggcgtcg agatcgacga ggcggcgctc
       61 gaggcgaccc tcgacgagtg gcgcaccggg ggcggcgcct tcggcgcgac tctgtcgatc
      121 cgggtgccag gccacgacga cgtccatctc gcctctgggg tggacaaccg cgacatcacc
      181 accagcggtc cggacggaac acccatcgag cgacgttcga cggatggggc tacacgcggg
      241 tgttcgacac ctcgaacccg gccgccccct ccgaggtcag tcagatcacc atcccggaga
      301 ccgccgacga ggacttcgcg atcggcttcg gggatctgac cgtccacgag gtcgaggtgc
      361 cgcgaggcga tcccaacgag ggcggccccg ggctcgacga cgacaaggtt gcgtacttct
      421 cctggtatgc cggggggttc cgagtggtgg acatcaccga cccgagcaac ccggaggagc
      481 tgggccacta catcgacgag aacggcaaca acttctgggg cgtggccctt gccgaggatg
      541 agaacggaga ccggatcgtg ctgggcagcg accgggactt cggtctgttc atcttccggt
      601 acacgggaac gatcccgtcc taggccgctc gctgcggaag ggcggtgggg ccggccgaaa
      661 ggccggcccc acccatcagg gcagttctca ctcagggccc cacctcgtga ctgtcgcggc
      721 cgtcgccgct gggcgcgcca tgatgaagct ccctacgtcg atcgtcagta ggagggaaat
      781 gtcatgtgcg cccccagagt tcgatggctg ctgtgcgtaa ccggaactgc ggcgctgctg
      841 atgatgaccg cgcttccggc cgcagccgat cctcccctgc atcagcggtt caccttctcg
      901 ggcgccgtaa cggacgaagg tcaagcctgc ggcgagccgc tgtcgtggga gttcaccggc
      961 gaggtgttgc tgagccgctt catcgacacg gagggcaacc tcgtccggat ccaggtccaa
     1021 gttcgggagg ccgggtcggt caccgacttg gcgaccggcg aggtcatcga gctgccgcgg
     1081 accgccttta ccgagcgggt cctcttcgtc gaggacggct cgatcatcgt tgaggatgtc
     1141 ggtttgtcgg tccgggtcac cggtcccgag gacttcctgc tcgacgtggg tcggttcgtg
     1201 gtgcgcttca accccgacga gttgctccag tccagcggtc agcatccaat ccgtgagatc
     1261 aacccgttct cggtggagga tccggcgctt cttggggctt tctgcgagct gttcgactga
     1321 gagagggaga cagaagggct gtcatggcga tagtcaccga tcccgtctgc gggatgggga
     1381 tcgatcccga cgacgcggcg gtgactgccg agtacgaagg gaatacggac tactttctgt
     1441 tctgaagcct gccaggatgc gtttgtggcg gatcccatct cgtacgtcgc ctgacgcatt
     1501 tcgggaacgg agcgcgacac ggtcaccgaa gacgagctgg cgaagcgtgc agggacgagc
     1561 atcgaacacg tccgagagct cgaggacctc gggatcctcg agtcctggca tgggacgttc
     1621 ccccgtcgcg acgtcatgcg agtgcgagtc gtagtggagc tcgaagcgga ggggatggac
     1681 gcagctgcac tcggggctgc cctggccacc ggacacctga ccctcgggta cctggagagc
     1741 gccggacgac ggttccctca gttagatcga aattctcccg gcgcagcgag gacatcggca
     1801 tcccgctgca gacgctgcag acgctgcacg cggtctatgt ggcgcgacgc cgcggcctcc
     1861 gtgataacga ggcgttcgag gctgccatcc gcgaagtcgg gttccgcatg ggcactcagc
     1921 ggactgctcg gcccaccggt ccgggacccc gtcggacagt cccgccggta gccgggacgc
     1981 tcagggtggc caccggaact gtcgatgagt gatcacc
//
LOCUS       DASUTA010000410         2435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1546006, whole genome shotgun sequence.
ACCESSION   DASUTA010000410 DASUTA010000000
VERSION     DASUTA010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546006"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VFZ80_08090"
     CDS             <1..409
                     /locus_tag="VFZ80_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287432.1"
                     /translation="TSGARSAFERVGPLDGLVDDVLRPGTLVIAADVLFALDSRSEAP
                     LVSRLLAPFRDRFIMVGTAFALLGPVQRSLALLESLASSPSDGLELLLTTARTCRGLG
                     MNSWADRSLQDARRIARRNDLPEPLSADLALHH"
     gene            479..1339
                     /locus_tag="VFZ80_08095"
     CDS             479..1339
                     /locus_tag="VFZ80_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287433.1"
                     /translation="MLLTGRPGEISKCIQLLSDSPVSHAILVLPEQQVIMAHLPRTAS
                     DSVVQRYTYSQLQAESWTAMYCQRHQGLDSDGRALSKIIEMAEMFERGEGPEGKLDYK
                     FGTADMVIGAVLCMLKPASKVIRLVTRYINGNVEQSLRKLFDDRGGPAQLFCSEFVYR
                     CFLDASQTVPSAAIDTSMLILREWAAIFTGQQPLMAVSNELSAPEAEMTVTWLDSADS
                     PTLVGPGISQQDDVQALLVAMRDFLRELDFQGSLGTIPGLAPSIADFVTPVDLLRSPT
                     FCTVAWWGTT"
     gene            complement(1404..1853)
                     /locus_tag="VFZ80_08100"
     CDS             complement(1404..1853)
                     /locus_tag="VFZ80_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287434.1"
                     /translation="MGQAPGRQDGSRLPVLGQVRKTTKEHVNGTPSWQSVVVALAIIG
                     LVGLFFVVGLTQTEDVDEFLKLWGGLGTIVGVVAGAIPSFFFKMQADKAVAQADRAIS
                     RAETATTTAMQLSERAQALAGVADAAAIERAVEMAPQAFQDWNPRPT"
     gene            complement(1906..2247)
                     /locus_tag="VFZ80_08105"
     CDS             complement(1906..2247)
                     /locus_tag="VFZ80_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287435.1"
                     /translation="MEGSALSPLAEIERAVQERAKDISLEMSGPDGEAKLRGLIADEV
                     ARWNDDHRRGRRALALPDPDAVVERAFRNLARHGPLTPLLDDDDVWEIMVNAPDSVFV
                     RSTTPTSGWAS"
BASE COUNT          436 a          798 c          737 g          464 t
ORIGIN      
        1 cacgtcggga gcccggagcg cattcgaacg cgtcggaccc ctcgatgggc tggtcgatga
       61 cgtgctccgg cccgggacgc tcgtcattgc cgctgatgtc ctgttcgcat tggacagtcg
      121 atccgaagct cccctggtgt cccggcttct cgcgcccttc cgtgatcgct tcatcatggt
      181 gggaacggcg ttcgcactgc tcgggccggt gcagcgttcc ctcgcactgc tggaatcact
      241 cgccagttct ccgtcagacg gcctggaact gctgctcacc accgcgcgca cgtgccgtgg
      301 cctgggaatg aattcctggg cggaccgttc gctccaagac gccaggcgca tcgcccgcag
      361 aaacgacctt cctgaaccgc tcagcgccga cctcgcactc caccactgag aagggggact
      421 gaccatgccg agatgtcagc agttgagcag tgtcctcgac catgcccgac ctggcgatgt
      481 gctgctcacc ggtcgaccgg gcgagatctc caagtgcatt caactgctct ccgactctcc
      541 ggtaagtcat gcgatccttg ttcttccaga gcagcaagtg atcatggcgc atctacccag
      601 aactgcgagc gatagcgtcg tgcaacggta tacgtacagc cagctgcagg ccgaaagctg
      661 gacggcaatg tactgtcagc gccatcaagg gcttgattct gacggccgag ccttatcgaa
      721 gattatcgag atggctgaga tgttcgagag aggtgaaggg ccagagggca aactcgatta
      781 caagtttggt acggccgaca tggtcattgg tgccgtcctg tgcatgctca aaccggcgtc
      841 gaaggtgatt cgcctggtga ccaggtacat caatggcaac gtcgaacaga gtctacgtaa
      901 gctcttcgac gaccggggcg gaccggctca attgttctgc tccgagtttg tgtaccgttg
      961 cttcctggat gcctcgcaaa ctgtgccgtc ggcggcaatc gatacgagta tgctcatcct
     1021 tcgagaatgg gcagccatct tcaccggcca acagcctctc atggcagtaa gcaacgaact
     1081 gtcagctccg gaagcggaga tgaccgtgac atggttggac agcgccgact ccccgacact
     1141 tgtcggtccc gggatcagtc aacaggatga tgtgcaagcg ctccttgttg cgatgcgcga
     1201 cttcctgcgg gaactcgatt tccaggggtc tctcgggacc atcccaggct tggctcccag
     1261 catcgcggac ttcgtgactc ccgtcgacct tctccgatct cccaccttct gcacggtcgc
     1321 ctggtggggc actacatgag ctgacgatcg ggcacggcgg actcggacct gcactccagc
     1381 agggtgtggg cacgtccggg cgatcacgtc ggtcggggat tccagtcctg gaaggcttgc
     1441 ggcgccattt cgacagccct ctcgatggcc gccgcatcgg caactcctgc gagagcctgc
     1501 gctcgttcgc tgagctgcat cgcagtcgtc gtcgcagttt cagcccgcga gatcgcccgg
     1561 tcggcttgag cgaccgcctt gtcggcctgc atcttgaaga agaacgaggg aatggcgccg
     1621 gcgacgacgc cgacaatggt tccgagacca ccccagagct tcaagaactc gtcaacgtcc
     1681 tccgtttggg tgagcccgac gacgaagaac agtccgacga gaccgatgat cgccagagcg
     1741 acgacgacgg actgccaact cggtgttccg ttcacgtgtt ctttggtggt cttgcggacc
     1801 tggccaagga ccggtagccg agacccgtcc tgtcggcctg gcgcttggcc cattcgtggc
     1861 cgttgatcca caccttggcc ggatacggga aggaggtgca gatcttcatg aagcccaccc
     1921 cgaagtcggg gtcgtggacc ggacgaacac cgagtcgggc gcgttgacca tgatctccca
     1981 cacgtcgtcg tcgtcgagca gcggcgtgag ggggccgtgc cgggccaggt tgcggaacgc
     2041 ccgctcgacc accgcgtcgg ggtcgggcag cgcgagggcc cggcggcccc ggcgatgatc
     2101 gtcgttccac cgcgccacct cgtccgcgat gagccctcgc agcttggcct caccgtcggg
     2161 cccggacatc tccagcgaga tgtccttggc acgctcctgc accgcccgct cgatctccgc
     2221 cagcggcgac agcgccgacc cctccatcgg atcccccctc tctctacgcc cagccgcttg
     2281 tgctggtgtc cacacactag tagcgcacca gcgaagaccg tactacatcg aggaatgtcg
     2341 agaaatctca ttaggcctcg gcagtaccgc gcgtccatcc cggcgcgagc ggagacgagc
     2401 gcgcagctcg tcggcatcgt ctcgcctacc cgctg
//
LOCUS       DASUTA010000411         2789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_769588, whole genome shotgun sequence.
ACCESSION   DASUTA010000411 DASUTA010000000
VERSION     DASUTA010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769588"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VFZ80_08110"
     CDS             <1..394
                     /locus_tag="VFZ80_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM protein"
                     /protein_id="HEX6287436.1"
                     /translation="DRHDLVGATDPVQMAIKLLIPRGSLLEDHPSITPHLDGYDDAAL
                     SWRWDFADPETALLQKELEAIAANASECGQATALTLNEMREAVARASGIALAPFRESS
                     PAPRLTESWYCCAEPTGAQAAALQIGRR"
     gene            455..1363
                     /locus_tag="VFZ80_08115"
     CDS             455..1363
                     /locus_tag="VFZ80_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6287437.1"
                     /translation="MKRFGDLTAVNDVSFEIEEGETYGLLGPNGAGKTTSISMIAGLL
                     EPDGGSVQVDGARITTRTTAGKANIGLVPQELAIYPDLTAAENLSFFGKLYGGGGAEL
                     QERVDQVLEVIGLTDRKDDLVKEYSGGMKRRLNIGIGLLHRPQLLILDEPTVGVDPQS
                     RNAILESVESLSAEGMAVLYTTHYMEEAERLCDRVAIIDEGVIQAEGTRRELVSMVGE
                     KDRVAIEATGDLAAVADIVRDIDGVTGVSASDRRIDVLATDASSILPQLLTCVSQSGG
                     SIVGVQVVEPNLEAVFLHLTGKALRD"
     gene            1364..2545
                     /locus_tag="VFZ80_08120"
     CDS             1364..2545
                     /locus_tag="VFZ80_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6287438.1"
                     /translation="MGAATRIAVKDLKLRVRDRSAIIIGIIAPLALAFVFDLVFGGAF
                     TQQLDLEFGLVDLDGTEISQGLGQGLAEVEAEGVLSVETYSSADVAEQAVDDGDIDAF
                     ILIEEGFGASVTSNSDATISVIGSVDAPTSTQIAESIANQYATGVGATQLALVTTAAV
                     SGSQIDPGFFASLSRDPSTAAFSYTLTDVTTATRQLDATTYFAAGMAVFFLFFTVQFG
                     VLGLLEEERQGTLARLQAAPISRASIVLSKALLAFLLGLISMGVLVVSTQLLLGASWG
                     APFGVALLVIAGVLSAVGIMGLVAAVAKTPEGAGNLGAVIAVILGMLGGVFFQLGSGD
                     DFLSRLTYITPHAWFMNGLAELADGASWTAALPSVGAIMVFAVVTGVVSWVLLNRRFV
                     L"
     gene            2581..>2789
                     /locus_tag="VFZ80_08125"
     CDS             2581..>2789
                     /locus_tag="VFZ80_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019482477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287439.1"
                     /translation="MLRERSNIFFVFVFPIAIILLIGAQFGGDFNPVVGFYAADDGEI
                     ASSVVEGLQSLNDIDTRSFETEDEL"
BASE COUNT          497 a          865 c          874 g          553 t
ORIGIN      
        1 cgaccgccac gatctggttg gggccacaga cccggtccaa atggcgatca aactgctcat
       61 ccccaggggc tcgttgctcg aggaccatcc ctcgatcact ccgcacctcg acgggtacga
      121 cgacgcggcg ctctcttggc ggtgggactt cgccgatccg gagactgcgt tgctgcagaa
      181 ggagttggag gcgattgctg ccaatgccag cgagtgtggc caggcaacgg ctctcactct
      241 gaacgagatg cgggaggccg tggcccgagc atcgggcatc gccctggcgc catttcgtga
      301 gtcgagcccc gccccgcggc tgaccgagag ttggtactgc tgtgccgaac cgaccggcgc
      361 tcaagccgct gcactccaga tcggacgtcg ttagagtcgc caggcccatc agggaggcaa
      421 gacactggcc cagattctga gagctgagaa cctcgtgaag cgcttcggcg acctcacggc
      481 ggtcaacgat gtcagcttcg agatcgaaga gggcgagacc tacggtctct tgggccccaa
      541 cggtgcaggg aagaccacct cgatatcgat gatcgcagga ctgctcgagc cggacggggg
      601 cagcgttcag gtcgacggcg cccgtatcac gactcggacg accgccggca aggccaacat
      661 cggcctggta ccccaggagc tcgccatcta tccggatctg acagctgcgg agaatctgag
      721 cttcttcggc aagctctatg gcgggggggg agccgaactg caggagagag tggaccaagt
      781 gctcgaggtc atcggtctca ccgaccgaaa ggacgacctg gtcaaggagt actcgggcgg
      841 gatgaaacgc cggctcaaca tcgggatcgg tttgctccat cgtccccagt tgctcatcct
      901 cgacgagccc acggtgggtg tggaccccca gtcccgcaac gcaatcctcg aatccgttga
      961 gagtctgtcg gcggagggaa tggccgtctt gtacacgacc cattacatgg aagaggcaga
     1021 gcgtctctgc gacagggtgg cgatcatcga cgagggggtt atccaggcgg agggaacccg
     1081 acgggagctg gtgtcgatgg tgggggagaa ggatcgggtg gccatcgagg caacggggga
     1141 tctcgctgcg gtcgccgaca tcgttcgtga catcgacggc gtcaccgggg tatcggcgtc
     1201 agatcgacgc attgacgtgc tggccaccga cgcctcctcc atactgcctc agctgctcac
     1261 atgtgtgtca cagtcaggtg ggtcgatcgt cggtgtccag gtggtggagc ccaacctgga
     1321 agccgtgttc ctgcatctga cgggcaaagc ccttcgggac tgaatgggcg ccgctacccg
     1381 aatcgcagtc aaagacctga agctgagggt tcgggatcgc tccgcgatca tcatcggcat
     1441 catcgcaccg cttgccctgg ccttcgtttt cgacttggta ttcggcggtg ccttcacaca
     1501 gcagctcgac ctcgagttcg gtctggtcga tctcgacggc accgagatct cccagggcct
     1561 gggacagggg cttgccgaag tggaggccga aggcgtgctc tccgttgaga cgtacagctc
     1621 ggccgacgtc gccgagcaag cggtcgatga tggagatatc gatgccttca tcctcatcga
     1681 agaaggcttc ggcgccagtg tgaccagcaa ctccgatgcg accatctccg tgatcggcag
     1741 tgttgatgca ccaacctcga cccagatcgc cgaatcgata gccaaccagt acgccaccgg
     1801 cgtcggtgcg acccagctcg cactcgtcac caccgccgca gtctcgggat ctcagatcga
     1861 tccagggttc ttcgcatcgc tgagccggga tccgtcgact gcggcgttct cctacaccct
     1921 caccgacgta acgacggcga cgcgacagct cgacgccacc acctacttcg cagccgggat
     1981 ggctgtcttc ttcctgttct tcacggtgca gttcggggtg ctcggcctgc tcgaggaaga
     2041 gcggcaggga acactggcac gtttacaggc tgcgccgatc agtcgggcct cgatcgtctt
     2101 gagcaaggct ctcttggcct tcctcctcgg cctcatctcc atgggcgtcc tggtcgtttc
     2161 cacccaactc ctgctcgggg cttcgtgggg ggccccattc ggcgttgcac tcctcgtcat
     2221 tgcgggcgtc ctctccgctg ttggcatcat gggcctggtc gccgctgtgg cgaagacccc
     2281 agaaggtgca ggcaacctgg gtgcggtcat cgcagtgatc ctcggaatgc tgggcggcgt
     2341 cttcttccaa ttgggatcgg gtgacgattt tctctctcgc ctcacataca tcaccccgca
     2401 cgcctggttc atgaatgggc tcgccgagct cgccgacgga gcttcgtgga cggctgcgct
     2461 tccctcggta ggggcgatca tggtcttcgc cgtggtcacg ggtgtcgtct cctgggtcct
     2521 gctcaatcgg aggttcgtcc tgtgagggtc tgggcgatcg gtctctcgaa catcaagcga
     2581 atgctgcgcg agcgatccaa catattcttc gtcttcgtct ttcccatcgc aatcatcctc
     2641 ctcatcggag ctcagttcgg tggtgacttc aatcccgtag tcggtttcta tgccgccgat
     2701 gacggtgaga tcgcctcatc ggtagtcgag gggctccaat ccctgaacga catcgacacc
     2761 aggagctttg agaccgagga cgagttgtt
//
LOCUS       DASUTA010000412         8249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1287888, whole genome shotgun sequence.
ACCESSION   DASUTA010000412 DASUTA010000000
VERSION     DASUTA010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287888"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..148)
                     /locus_tag="VFZ80_08130"
     tRNA            complement(76..148)
                     /locus_tag="VFZ80_08130"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(113..115),aa:Lys,seq:ttt)
     gene            297..1238
                     /locus_tag="VFZ80_08135"
     CDS             297..1238
                     /locus_tag="VFZ80_08135"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEX6287440.1"
                     /translation="MPEEVPDESGPVEVPEPEQSRWREYWATLRVRAYEKMTTAEQRR
                     QSSKVYDAAFRVYERNRLLPASVLVGALASRIVVYLIPLLALLVFSFGFYEDVGGSGT
                     EAASSIAGVFAVAVEDSRDVSEGFRFAAVIATLFAVLYAANSLGRLVRRATALIWGVP
                     YVRSKKPWTPPIAVLAISALALLITSLGSWSDEWTWQATLGAVIIEFVLLTTLWVFVS
                     RALPHDPAASKLSHFVPGALFVALGIVGLRLAMVIYFVPQSADLSQRYGSIGVALVML
                     TWAYWLGMIIVGSSEVNAALLRATSGREGAERTRESA"
     gene            complement(1285..1824)
                     /locus_tag="VFZ80_08140"
     CDS             complement(1285..1824)
                     /locus_tag="VFZ80_08140"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HEX6287441.1"
                     /translation="MTNTALLDSIPRPIDPGAEMDALGPFHRDGTWSGTIHAGGMGPG
                     TPTQLARGKAAHRWIQDGRWVVGDYEQDQLLDDGTFVLKWELHWVCGWDPMASEYKAT
                     IADNYGRAALLHGRIEGDAMIFESTDASPPFLRLTWELGPGGKILWRNEISMDGEAWI
                     LVEDYEITPTDQSAVHTSG"
     gene            complement(1821..2720)
                     /locus_tag="VFZ80_08145"
     CDS             complement(1821..2720)
                     /locus_tag="VFZ80_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287442.1"
                     /translation="MSVITSERSPTVATGEPAHLPRHAYVLSLLLVAVGGLTAALTFF
                     VPDVLQGPPVTNGNARGTALVMLALATPALLTSMLMEANGSRRARFIWLGSLFYLAYN
                     AFLLLFLTPFNSLFLLTIATESLALFSIFALVFASRGFPSASEAQRIPVRGLAIFVWV
                     IVLLNTLAWLQVVVPAVLSDEPGSFLDGLGVATNAIYVQDLVLWLPLMATAAWWMWQR
                     RPIGIFLTGSWLAFGFAESIGIAVDQWFGHQADAVSPHASTEVIPLMIGLALINLVGL
                     FFYLRRSGRVAREAVVFSHKEGS"
     gene            complement(2813..3571)
                     /locus_tag="VFZ80_08150"
     CDS             complement(2813..3571)
                     /locus_tag="VFZ80_08150"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX6287443.1"
                     /translation="MSDPTSLREKETAYGLWSVTGHPAIVDAAASTGPDFVVVDTQHG
                     VDLGRLDASLFTVLAHYGVAGLVRVPAIHPTPIGRALDLGAAGVIVPLVESAEDAEQA
                     VAATRYAPDGRRSYGMQTHRVGAFDHAPFVAIQIETASAVGYVDAIAAVDGVDALYIG
                     PADLGLALGGETAADVNAVFDGTHPLSDKLNAAFSAVVAECRGHGIIAGLHVGNGTSA
                     ARAREHGFTFASVSADVGLVADSLARELHAARES"
     gene            3605..5422
                     /gene="ligD"
                     /locus_tag="VFZ80_08155"
     CDS             3605..5422
                     /gene="ligD"
                     /locus_tag="VFZ80_08155"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX6287444.1"
                     /translation="MSSLRFGISELPPDDGDDGAFLDDLVAQGHRALELPFTQGFPWK
                     EKRCEAFAELAAERDIRLSVHAPYFVGLTIPEEEKGTQSVHALEHTMKLGKWLGAPVI
                     VAHFGSTYEEEPQVLMDRIRSRIDFIAPKIESLGVGLGLETSGKRSQFGSLGDIAMLA
                     SEYNFVRPVVDWAHVHAMTGGGLTSKEAFMSVLSFVRDSFPGWMVSPLQVQFSDNQFG
                     EVGEIRHVAYGEGTLRVGPLVEAVEELGMSMVMISEARDMKSHGLIWDEVKAAMSSGT
                     RPTGRPLSTASSEFPSPIRVTKEGDRFIPADVDRPITFSNVDKVFFPDAGYTKGDLIQ
                     YYASVAPLLLPHLEGRPISMSRYPDGINGQSFYEKRAPGHQPDWMRTTPVVSDSQGGI
                     IDFLLADSPEALMWFANMGCIEFHPFHSKAADLDKPTYAIFDFDPAEGSTWDQVTGAA
                     QLLKLALGQLGLEGYPKLSGSRGLHVYVPLDPVHSHSRVRRFVGEVGEYLAAANPDDV
                     TMEWDKPKRKGKVFVDHNRNAFGQTVASVYSVRPRPGAPVAAPLTWDEVGTVANAELT
                     IVNIWNRLQRYGDLFAPVLEGGQTLDAAEEALGISSEES"
     gene            5539..6609
                     /locus_tag="VFZ80_08160"
     CDS             5539..6609
                     /locus_tag="VFZ80_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX6287445.1"
                     /translation="MDLYARVNILGGLSVRLPRGGLDDAIPLDNDPIGRAKNWVQQGV
                     DYLHIVDLDAAAYGDYRNRDLIRRLIEEIEIPVQVAGGIRSEGEGEKLLETGAWRIVM
                     GTAAIENQLMVWEMCRDHPGRIVVSVDVRPNEEIATRGWTQNSGRFLEEVLIEMSSAG
                     AVAFLVAEAGRDALSEPPNLQILSEALATVEDPVIAAGGVRDLEDLRKLMLLETSGRH
                     LAGVVVGREVTAGRFTIDEAKQAIAGGGPVRAPGGIQTTRTPVGVASLTASLEFYRDK
                     LGFNQVRSPSQGVIAAVVEAGPGQHIELVEGGSARPDALVFNVEDPERWKVHLTNLGV
                     EATADGPRLILNDPDGLRIVLE"
     gene            complement(6681..8234)
                     /locus_tag="VFZ80_08165"
     CDS             complement(6681..8234)
                     /locus_tag="VFZ80_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6287446.1"
                     /translation="MAVRITYATMSADNQELNEAYEKAVADADSKLGQTHGVIVNGES
                     RTDREMFEELSPIDSDILVGRYAQATDQDIDDAIAAAKAYQPEWEAMGWQARRDLMLH
                     AAEVMEGQVFDLGALMTFEVGKNRLEALGDAAETVDFLRYYSKSITDNNGFLRELSAL
                     DDSEENVSVLRPWGVWGVISPFNFPLALAAGPSIGALITGNTVVVKPSNAGALMALEF
                     YRVMREAGLPPGALHVVTGGDQAGDYLAHHDGLDGLTFTGSHEVGMYLYRTVNERRPK
                     PVTAEMGGKNPAIVTKSADLDTAAEGIVRGAFGFSGQKCSATSRVYVEDDVYDEFIDK
                     LVSRTEQVTVGLPAENGVFMGPVIDRAAVERFREAVSEAESSGRVLTGGKVKDDGDYA
                     RGNFVEPTVVTAPKDSWLWEKELFVPFVAVTKVEDLDEGLRLSNDTPFGLTAGLFSND
                     EEEIDRWFSGIQAGVTYVNRAAGATTGAWPDIQSFGGWKGSGTSGAGGGGPWYLRQFL
                     REQSRTRVR"
BASE COUNT         1501 a         2584 c         2638 g         1526 t
ORIGIN      
        1 gtggtcactc cgcgcaccaa tgcgatggcc cactggcggg gggtgccggg gggtagtccc
       61 cctgcaatgt cacagtgggc cccgtgggac tcgaacccac aaccaacggg ttaaaagccc
      121 gctgctctgc caattgagct agagacccgg agtcccctcg tccagggtac cgcgcacggt
      181 cctggatcaa cagaccgtgg aatgggtcag gcggtccagg cggtctccac gaatagtctc
      241 aacagcgtct ccacccaggt atcttccgtc gtccgaatgg gatcgaaagg agcgcggtgc
      301 ctgaggaagt ccctgacgag tccggacctg tggaggttcc tgagccggag cagagtcggt
      361 ggagggaata ctgggcgacg ctccgggtca gggcctatga aaagatgacc actgctgagc
      421 agcgtcgtca gagctcgaag gtatacgacg ctgcgtttcg ggtctacgag cggaaccggc
      481 tcctgcccgc ctcggtgctg gtcggtgcct tggcgagccg gatcgtggtc tacttgatcc
      541 cccttctcgc actcctcgtc ttctcgttcg ggttctacga agacgtcggc ggctcaggga
      601 ccgaggccgc cagcagcatc gccggagtgt ttgccgtggc tgtcgaagac tcgagagacg
      661 tgagtgaggg gtttcgcttt gccgcggtca tcgcaactct cttcgcggtt ctctatgcag
      721 ccaacagcct gggtcggctg gtgagaagag cgaccgccct catctggggc gtcccgtatg
      781 tgcggtcaaa gaagccgtgg acgccgccta ttgctgtcct cgccatctca gcgctggctc
      841 tgttgattac gagtcttggc tcctggtcgg atgagtggac ctggcaggcg acactcggtg
      901 ccgtgatcat cgagttcgtg ctgctgacca cgctttgggt gttcgtcagt cgggccttgc
      961 cgcatgaccc cgctgcctcg aagctgtccc acttcgtgcc cggtgcccta ttcgtcgcac
     1021 taggaatcgt gggcttgcga ctggcgatgg tgatctactt cgtcccacag agcgcagacc
     1081 tctcccaacg ctacgggtcg atcggcgtgg cactggtgat gctgacctgg gcctactggc
     1141 tgggcatgat catcgtgggg agcagcgagg tgaatgccgc attgcttcgg gccacgtcgg
     1201 gcagagaagg agccgaaagg actcgcgaat cagcctgacc aggtgtgtgg cgcttggtgg
     1261 caccgaatgt tgccctttga ggactcaccc tgaagtgtgg accgccgatt ggtcggttgg
     1321 ggttatctcg tagtcctcga cgagtatcca ggcctctccg tccatgctga tctcgttgcg
     1381 ccacaggatc ttccccccgg gcccgagctc ccaggtcagt ctgagaaatg gaggggaggc
     1441 gtcagtcgac tcgaagatca tcgcgtctcc ttcgatgcga ccgtgaagga gggctgcgcg
     1501 cccgtaattg tcggcgatgg tcgccttgta ctccgaggcc atcgggtccc acccacagac
     1561 ccagtgcagc tcccatttca gaacgaaggt cccgtcgtcc aggagttggt cctgctcata
     1621 gtccccaact acccaacgac catcctggat ccatcggtgt gccgctttgc ctcgtgccaa
     1681 ctgtgtcggt gtgcctgggc ccatcccacc ggcatggatg gtgccgctcc acgttccgtc
     1741 acggtggaag ggcccgagag catccatctc cgcacccggg tcgataggtc gggggatcga
     1801 atcgagtaat gcggtgttgg tcatgagccc tccttgtggc tgaatacgac ggcctcgcga
     1861 gcaactcgcc cgctacgacg cagatagaaa aagagaccga ccagattgat caaagccagc
     1921 ccgatcatga gtgggatcac ttcggtcgat gcgtgaggtg acaccgcatc ggcctggtgg
     1981 ccgaaccact ggtcgacggc gatcccgatc gattcggcga acccgaaggc gagccacgaa
     2041 ccggtgagga atatcccgat cggtcgtcgc tgccacatcc accacgccgc ggtagccatc
     2101 agcggaagcc agaggaccag gtcctggacg tagatggcgt tggtggccac ccctaggcca
     2161 tcgaggaagg agccaggctc atccgagagg acagccggca cgactacctg gagccaggcg
     2221 agcgtgttca gcaacacgat cacccagacg aagatggcca gaccacggac cgggattcgt
     2281 tgagcttcac ttgcggaagg aaagccccga cttgcgaaga ccaaggcgaa gatcgagaag
     2341 agtgccagag actccgtggc gatcgtgagg agaaagaggc tgttgaaagg ggtgaggaac
     2401 aacagcagga acgcgttgta agcgagatag aagagagagc cgagccagat gaacctcgca
     2461 cgtctcgatc cattggcctc catcaacatc gatgtcagca atgcgggcgt cgccagagcg
     2521 agcatgacca aggccgtgcc tcgggcgttg ccgttggtca ccggtgggcc ctgaagcaca
     2581 tccgggacga agaaggtgag agcggcggtg agaccgccaa ccgccaccag tagaagcgac
     2641 agcacatatg cgtggcgggg taagtgagca ggctcgcccg tcgcaacggt cgggctcctc
     2701 tccgacgtga tcacactcat tggacggctc cttccggcgc gaagtcccta tcgaagggat
     2761 tgtccaactg aggatcgacc gtggttaggg gcctaagtcc tgattcgcca ggtcaggatt
     2821 ccctggcggc gtgcaactcc ctcgccaggc tatcggcgac caacccgacg tcggcggaca
     2881 cggaggcgaa ggtgaaacca tgttcgcggg cccgggccgc gctcgtgcca ttcccgacat
     2941 ggagcccggc gatgattccg tgcccgcggc actcggccac gacggcggag aacgctgcgt
     3001 tgagcttgtc cgaaaggggg tgggtgccgt cgaagacagc attgacgtcg gcggcagtct
     3061 ctccacccag cgccagaccg aggtccgccg gtccgatgta gagcgcatcg accccatcga
     3121 cggcggcgat cgcatcgaca tagccgacgg cagacgcggt ctcgatctga atcgcgacga
     3181 acggggcgtg atcgaaggcg ccgacgcgat gggtctgcat cccgtaggag cgtcgcccgt
     3241 cgggggcata ccgggttgcg gccacggctt gctctgcgtc ctcggcggat tcgaccaggg
     3301 gaacgatcac gccggcggca cccaggtcga gggcgcgacc gatcggtgtc gggtgtatcg
     3361 caggaaccct gaccaggccg gcaaccccat agtgggccag tacggtgaac agggaggcat
     3421 ccaatcgccc caggtcgaca ccgtgctggg tgtcgaccac cacgaagtcg gggcctgtcg
     3481 aggcggcggc atccacgatc gcaggatggc cggtcaccga ccacaggccg tatgcagtct
     3541 ccttctcgcg caaagacgtt gggtccgaca tggcgttcta cgttacgcct ggcaggattc
     3601 gagcatgagc tcgcttcgct ttgggatctc ggagttgcct cctgacgacg gggatgacgg
     3661 cgccttcctc gacgatctcg tcgctcaggg ccaccgtgcc ctcgagctcc ccttcactca
     3721 gggtttcccc tggaaggaga agcgatgcga ggcattcgcc gagctggcgg cggaacgcga
     3781 catccggctc tcggtgcatg ccccgtactt cgtcgggttg accattcccg aggaggagaa
     3841 ggggacccag tcggtccatg cgctcgagca caccatgaag ctggggaagt ggctgggcgc
     3901 cccggtgatc gtcgcccatt tcggatccac ctatgaggag gaaccccagg ttctcatgga
     3961 caggattcgg tcccggatcg acttcatcgc cccgaagatc gaatcacttg gtgtcggcct
     4021 gggactggag acctcgggga agaggagtca gttcggcagt ctcggtgaca tcgccatgct
     4081 ggcgtcggag tacaacttcg tccgaccggt cgtcgattgg gcccacgtcc acgccatgac
     4141 tggaggcggt ctcacctcga aggaggcgtt catgtcggtg ctttcgttcg tccgtgacag
     4201 ctttcccgga tggatggtgt caccactcca ggtgcagttc tccgataacc agttcggtga
     4261 ggtcggcgag atcagacacg tggcgtacgg ggagggcact ctgagagtgg ggcctctggt
     4321 cgaggcggtc gaggagttgg gcatgtcgat ggtgatgatc tccgaggctc gagatatgaa
     4381 gagtcacggc ctcatctggg acgaggtgaa ggccgcgatg tcgtcgggga cacggcccac
     4441 cgggcgccct ctgtccacgg cgagctccga gttcccctca ccgatccggg tcaccaagga
     4501 gggcgaccga ttcatacccg ccgacgtcga tcgtcccatc accttctcca atgtggacaa
     4561 ggtgttcttc cccgatgccg gatacaccaa aggcgatctg atccagtact acgcctcggt
     4621 ggcaccgctt ctcctgcccc atctcgaagg tcgcccgatc tcgatgtccc gttaccccga
     4681 tggcatcaac ggccagtcct tctatgagaa gcgggccccg gggcaccaac ccgactggat
     4741 gcggaccacg ccggtggtgt ccgactccca gggcgggatc atcgatttcc tcctcgccga
     4801 ctcgcccgaa gccctcatgt ggttcgccaa catgggttgt atcgaattcc accctttcca
     4861 ctccaaggct gccgatctcg acaagcccac atacgcaata ttcgacttcg accctgccga
     4921 aggctcgacc tgggatcagg tcaccggggc agcccagctt ctgaaactgg ctctgggaca
     4981 actcgggttg gagggatacc ccaagctctc cggctcccgg gggctgcacg tctacgttcc
     5041 tctcgacccg gtgcacagcc actcgcgggt gcgtcggttc gtcggtgagg tgggcgagta
     5101 cctggctgcg gccaacccag atgacgtgac aatggagtgg gacaaaccga aacggaaggg
     5161 gaaggtgttc gtcgatcaca accgcaacgc cttcggtcag acggtggcgt ccgtgtactc
     5221 ggttcggccc cgccccgggg ccccggtggc cgccccgctc acctgggacg aggtgggcac
     5281 ggtcgccaac gccgagctga ccatcgtgaa tatttggaac cgtcttcagc gttacggcga
     5341 cctgttcgca cccgtcctcg agggcgggca aaccctcgac gccgcggagg aggcattggg
     5401 aatcagctcc gaggaatcct gaacggcgaa catgagctga tttgggccat atccggcctg
     5461 aggtaccccg ggttcgtggg tttgcgttgc gcgaactgtc gtttccatcc gctcgccggc
     5521 ggtcggtatg gttacggcat ggatctgtat gcccgcgtca acattctcgg tgggctttcg
     5581 gtccgacttc cccggggtgg cctcgacgac gcgataccgc tggacaacga tccgatcggc
     5641 cgtgccaaga actgggtcca gcagggggtc gactacctcc acatcgtcga tctcgacgcc
     5701 gccgcatatg gcgactatcg caaccgtgac ctgatcaggc ggctcatcga ggagatcgag
     5761 atcccggtgc aggtggccgg aggcattcgc tcagagggtg aaggagagaa gctcctcgag
     5821 accggggcct ggcgcatcgt catgggcact gccgcaatcg agaatcagct catggtctgg
     5881 gagatgtgcc gtgaccaccc gggaaggatc gtggtttcag tcgacgtccg acccaacgag
     5941 gagatagcca cccgagggtg gactcagaac agcggtcgct ttctcgaaga agtgctgatc
     6001 gagatgtctt cggcgggggc ggtcgctttc ctggtcgctg aggccggtcg ggacgccctc
     6061 agcgagcccc ccaacctcca gatcctctcc gaggccctgg caaccgtcga ggacccggtc
     6121 atcgccgctg gcggagtccg cgatctcgag gatcttcgca aactgatgct tctcgaaacg
     6181 tccggacgtc atctcgccgg agtggtcgtt ggccgcgaag tgacagctgg aaggttcacc
     6241 atcgatgagg ccaagcaggc catcgccgga ggcggcccgg tgcgggcgcc cggcggaatc
     6301 cagaccacac ggacccctgt cggcgtcgcc tctctaaccg catcgctcga gttctatcgg
     6361 gacaagcttg gcttcaacca ggtccgctca ccgtctcagg gtgtcatcgc cgcggttgtc
     6421 gaggcggggc cgggtcagca catcgagctc gtcgaaggtg gatcggcccg acccgacgcc
     6481 ctggtcttca atgtcgagga cccggagaga tggaaggttc acctcaccaa tctcggagtg
     6541 gaggccacgg ccgatggccc tcggctgatc ctcaacgacc cggacgggct caggatcgtc
     6601 ctcgaatagg tcttccccgc ttctgtgtcg gaaaagtggt ccgagagacc acaaaatcga
     6661 caccgaacgg tctgggccag ctagcggacc ctcgtccggg actgctcgcg taggaattgg
     6721 cggaggtacc aggggccacc gccgccggct cccgaagttc cagagccctt ccagccgccg
     6781 aacgactgga tgtcgggcca ggccccggtg gtggctccag cggcgcggtt gacgtaggtc
     6841 acacccgcct ggatgccgct gaaccagcgg tcgatctcct cctcgtcgtt ggagaagaga
     6901 ccggcggtga ggccgaacgg ggtgtcgttc gatagccgga gaccctcgtc gaggtcttcg
     6961 accttggtga cggcgacgaa ggggacgaag agctctttct cccagagcca ggaatccttt
     7021 ggagcagtca cgacggtggg ctcgacgaag ttccccctgg cgtagtcacc gtcgtccttg
     7081 acttttccgc cggtgagaac gcgaccgctc gactccgcct cggacacagc ctcccggaac
     7141 ctctcgaccg cagcacggtc gatcacgggc cccatgaaga ctccattctc ggcgggcaag
     7201 cccaccgtga cctgttcggt gcgactgacc agcttgtcta tgaactcgtc gtagacatcg
     7261 tcttctacgt agacacgcga tgtcgccgag cacttctgcc cgctgaagcc gaaggcaccg
     7321 cgcacgatcc cctcggcggc cgtgtccaaa tcggcgctct tcgtgacgat cgcggggttc
     7381 ttgccaccca tctcggcagt gaccggcttc ggtcgtcgct cattgaccgt gcggtagagg
     7441 tacatgccga cttcgtgcga tccggtgaag gtgagcccgt cgagaccgtc atggtgggcc
     7501 aggtagtcgc ccgcctggtc accgcccgtg acgacatgaa gcgccccggg cggaagccct
     7561 gcctccctca tgaccctgta gaactcgagc gccatcaagg caccggcatt cgacggtttg
     7621 acgaccactg tgtttcccgt gatgagcgcc ccgatcgatg ggcccgccgc aagggccaat
     7681 gggaaattga acggcgatat gacgccccaa accccccatg ggcggagcac cgagacgttc
     7741 tcctcgctgt cgtcgagagc gctgagctcg cgcaagaagc cgttgttgtc tgtgatcgac
     7801 ttcgagtaat agcggaggaa gtcgaccgtc tcggccgcgt cccccagggc ctcgaggcgg
     7861 ttctttccaa cctcgaatgt catcagagcc ccgaggtcga agacctgtcc ttccatcacc
     7921 tcggcggcgt gcagcatcag gtctcggcgc gcctgccacc ccatcgcctc ccattccggc
     7981 tggtacgcct tggcggctgc gattgcatcg tcgatgtcct ggtcggtggc ttgggcgtat
     8041 cggccgacga ggatgtcgct gtcgatgggg gagagctcct cgaacatctc acggtcggtg
     8101 cgactctcgc cgttgacgat cacgccgtgg gtctgaccca acttcgaatc ggcgtcggcg
     8161 acggccttct cgtaagcctc gttcaactcc tggttgtcgg ccgacatggt ggcgtacgtg
     8221 attctcactg ccatctctca acagtcctc
//
LOCUS       DASUTA010000413         4243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_509689, whole genome shotgun sequence.
ACCESSION   DASUTA010000413 DASUTA010000000
VERSION     DASUTA010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509689"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFZ80_08170"
     CDS             <1..547
                     /locus_tag="VFZ80_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6287447.1"
                     /translation="EVCEGKRFQAEVLEYELGGKNISEVFEMSVTEAEGFFADGESKV
                     PKAHATLERMAAVGLGYLKIGQPLTTLSGGERQRLKLAIHMADKGGIYILDEPTSGLH
                     LADVENLLDLLDRLVDSGKSVIVIEHNQAVMAHADWIIDLGPGAGHDGGRVVFEGTPA
                     DLIADGSSLTGKHLAGYVGSA"
     gene            complement(580..834)
                     /locus_tag="VFZ80_08175"
     CDS             complement(580..834)
                     /locus_tag="VFZ80_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX6287448.1"
                     /translation="MATNIFVGNLPFSTGSDDLETLFGRHGAVSRAQVITDRDTGRSR
                     GFGFVEMDDDAEAQAAIEALDGSEVDGRQIRVNVARERSR"
     gene            1064..1687
                     /locus_tag="VFZ80_08180"
     CDS             1064..1687
                     /locus_tag="VFZ80_08180"
                     /inference="COORDINATES: protein motif:HMM:NF038065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEX6287449.1"
                     /translation="MGITTHRALRLALASLLAVALTAQYIIGSTDSGLTGVNFFSYFT
                     VLSNAGAVVVLTMLAARPERDGSVPFAIFRGAVTVYMAVTGLVYAVVLYPQLADVAVP
                     EPWIDVCIHVVGPVLVVADWIYDSPPVELPGSSVAIWLAFPAVYLTYSLIRGPIADWY
                     PYPFLDPATGGWDAVALWSLVVLVVILAFSYAFQWWSNRRQSETALA"
     gene            1765..2658
                     /locus_tag="VFZ80_08185"
     CDS             1765..2658
                     /locus_tag="VFZ80_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6287450.1"
                     /translation="MNTYVVESGDGLLLIDCGTDWDPGRRALSEGFAALSLDESAVHT
                     LVVSHLHPDHVGMSARLVRELGCRFVMHERASRLVDRYNDTPGYARRLTELAGRHGMP
                     HSLVTSATSGARAEWMPLIDPPDHVVADGDEIELGNGRALSVVHTPGHEQAHICLRDS
                     RTGVVFSGDHVLPRISPVIMYDEEVGDPLGDYLASLHKLLTSGIGLTYPAHGTLIDRG
                     DERARQILLHHDRRLRDMAELVAHADTTAWTVVTKSFRPNLSPTDARLAFLETISHLE
                     HLRLEGRIEVDDRDGHLFYRR"
     gene            2735..2911
                     /locus_tag="VFZ80_08190"
     CDS             2735..2911
                     /locus_tag="VFZ80_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016812228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287451.1"
                     /translation="MIFGYLDAGSGSLILQAILGGAAGILVAFRAWKARLMGKQTAAE
                     GDAETNEQAVPSDS"
     gene            2918..>4243
                     /locus_tag="VFZ80_08195"
     CDS             2918..>4243
                     /locus_tag="VFZ80_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX6287452.1"
                     /translation="MSHADPGSFRDPASHVVLDDSRVIRLLDERGLEGWKALSGTPFF
                     DRALADGRLIESKEIPPEDATAAGALEHPKIPFISYPYEWTFSMLKDAALLQLDLLGE
                     ALADGMTIKDATPYNIQFIAGKPVFIDIGSFESYSPGEPWIGYRQFTRQFLFPLMLRA
                     WAGLPFQPWLRGQMEGPTAGDMKGILPLGKRMRPSAMMHVSLQARMEERMSGAAVRSD
                     LKTAGFSADLILANVRKLKKLVSSLNWEPSGEGWVEYETCGHVGRDRDRKGTFLLSAI
                     DAHGPSRVLDLGANDAYFSEMATANGALAIAVDGDEEVLDAVYRRGSGVSIVLGDLTN
                     PSPSQGWAGMERPSLDERARPDLLVAYGLIHHLIYTASIPPRSVIDWLASYECPVVLE
                     FVDPSDEMVAKLTANKTEEELHPGRTRYEFESIVATRFRTLDMMELGGGV"
BASE COUNT          754 a         1377 c         1300 g          812 t
ORIGIN      
        1 cgaggtatgt gaaggcaaac gctttcaagc ggaagtgctc gagtacgagc tgggcggcaa
       61 gaacataagc gaagtcttcg agatgtccgt gaccgaggcg gaaggattct tcgcggatgg
      121 ggagtccaag gtgccaaagg cgcatgccac cctcgagcgc atggccgccg tcgggctcgg
      181 ctacttgaag ataggtcaac cattgacaac gctctcaggg ggcgaacgcc agcggctgaa
      241 gctcgccatc cacatggccg acaaaggcgg catctacatc ctcgacgagc cgaccagcgg
      301 ccttcatctg gccgatgtgg agaatctcct cgatctcctc gaccgactgg tcgactcggg
      361 gaaatcggtg atcgtcatcg agcacaacca ggctgtgatg gcccacgcag actggataat
      421 cgatctaggg cccggcgccg gtcacgacgg tgggagggtc gtcttcgaag gaacgcctgc
      481 ggacctcatc gcagacggtt cctccctcac cggaaagcac ctcgccggtt acgtggggtc
      541 tgcctagggc gcgcctcgaa cggcgacgcc ggcgagtcgc tagcgggacc gctccctggc
      601 cacgttgacc cggatctgac ggccatcgac ctcggatccg tcgagtgcct cgattgcggc
      661 ctgggcttct gcgtcgtcgt ccatttcgac gaagccgaaa ccgcgggatc gcccggtgtc
      721 gcggtcggtg atcacctgtg ctcgtgacac cgcaccatgt cggccaaaga gtgtctcgag
      781 gtcgtcggaa ccggtcgaga acgggaggtt cccaacgaat atgtttgttg ccattgctct
      841 cctatgtttg cttcccgtcg agtctcttgc accaagcgcc gggaccggcc ggagccggtc
      901 gcctcttgca gtctccgacg ggtggagcgc acaggccagg tgtggcacat ctatgagtgc
      961 cggccctgag gctaatgact ccgaagcgaa aacgcaccca agttcttgac cctttgcctc
     1021 gtgtcaccac cgaaaggatc gaggacttag catcccgaac cgaatgggca tcacgacaca
     1081 ccgagctctc cgactcgccc tggcgtcatt actcgccgtg gcgttgaccg cccagtacat
     1141 catcggctcg accgactcgg gcctcaccgg cgtcaacttc ttcagctact tcacggtctt
     1201 gagcaatgcc ggcgccgtcg tggtcctcac catgctcgcc gcacgccccg agcgcgacgg
     1261 ctcggttccg tttgcgatct ttcgcggagc cgtcaccgtc tatatggcgg tgacgggtct
     1321 cgtctatgcg gtggtcctct atccccagct cgccgacgtt gctgtgcccg agccatggat
     1381 cgatgtctgc attcacgtgg tgggaccggt gctcgtcgtg gccgactgga tctacgacag
     1441 cccgcctgtc gagcttcccg ggagctccgt ggccatctgg ctggcattcc cggctgttta
     1501 cctgacgtac tcgctcattc gcgggcccat cgccgactgg tatccctacc cgttcttgga
     1561 tccggcaacg ggcggttggg atgcggtggc cctctggtcg ttggtggtgc tggtagtgat
     1621 cctcgcgttc tcctatgcct tccagtggtg gagcaatcgg cgccaaagcg agacggcact
     1681 cgcgtgagca caccgccgcc acgatcgctc ggcgatggca ttcacctcat tcctgctccc
     1741 ctgcccttcg attcgccccc atgggtgaac acatacgtcg tcgagtccgg cgacgggtta
     1801 ctactgatcg actgtggcac tgattgggat ccgggacgca gggctctctc cgaaggcttc
     1861 gccgcactga gtctcgacga gtcggcggtg cacaccctcg tcgtgtctca cctacatcct
     1921 gaccacgttg ggatgtctgc ccgcctggtc cgcgagttgg gctgtcgctt cgtcatgcac
     1981 gagcgcgctt cgcgtctcgt cgatcgatac aacgacactc cgggttacgc ccgccgcctc
     2041 accgagctcg cgggtcgcca cggaatgccc cactccctgg tgacctcggc cacgtcggga
     2101 gccagggctg agtggatgcc actcatcgat ccgcccgatc acgtcgtcgc cgatggagac
     2161 gagatcgagc tgggaaacgg aagggcgctg agcgtcgtgc acacccccgg ccatgagcaa
     2221 gcccacatct gtctccgcga cagccgaacc ggagtggttt tctccggtga ccacgtactc
     2281 ccccggatct cacccgtcat catgtacgac gaggaggtgg gtgatcctct cggggactac
     2341 ctggcctcgc ttcacaaact cctgacgtcg gggatagggc tcacctatcc ggcgcacgga
     2401 accttgatcg accgtggtga cgagcgggcg cgccagatac tccttcacca tgaccggcgc
     2461 ctgagggaca tggccgaact ggtggcgcat gccgacacca ccgcctggac ggtcgtcacc
     2521 aaatcgttcc ggcccaatct gagccccacc gatgccagac tggcgtttct cgagacgatc
     2581 tcacacctcg agcatctcag actcgaggga cgcatcgaag tcgacgatcg ggatgggcac
     2641 ctcttctata ggcgttgagg ggttggcatg cggctcgagt tgtacgaata tgtgtccgca
     2701 gcggtcgatc cgctcaccct gggaaggaac tcaaatgatc ttcggatacc tcgatgccgg
     2761 gtcaggcagt ctgatcctgc aggcgatact cggcggcgcg gcgggcattc tcgtcgcctt
     2821 cagagcatgg aaggcacgcc tcatgggcaa gcagaccgcc gctgaaggcg acgccgagac
     2881 aaacgagcag gcagttccct ccgactcctg accggaggtg tcgcacgccg atccagggtc
     2941 tttccgggac ccagcgtctc acgtcgtcct cgacgacagc cgtgtcatcc gtctcctcga
     3001 cgaacgtggc ctcgagggct ggaaggccct gtcgggcacc cccttcttcg atcgggcgct
     3061 tgctgacggc cgtctgatcg agagcaagga gatacccccc gaagatgcga cggcggcagg
     3121 ggccctggag catcccaaga tccccttcat ctcctatccg tacgagtgga cgttctcgat
     3181 gctcaaggat gcggcgctac tccagctcga cctgctcggt gaggcactcg ccgacgggat
     3241 gaccatcaag gacgccacgc cgtacaacat ccagttcatc gccgggaaac cggtcttcat
     3301 cgacatcggg tcgtttgaga gctactcgcc gggcgaaccg tggatcggtt accgccagtt
     3361 caccaggcag ttcctgtttc cgctgatgct ccgagcttgg gcgggtctgc cgttccaacc
     3421 atggctgcgt gggcagatgg aggggccgac tgcaggcgac atgaagggca tccttcccct
     3481 gggaaaacgc atgcgtccct cggcgatgat gcatgtatcc ctgcaggccc gcatggaaga
     3541 gcggatgtcg ggcgccgcgg tccgcagcga tctgaagaca gcggggttca gcgccgatct
     3601 catcctcgcc aatgttcgaa agctcaagaa gctcgtttcg tcgttgaatt gggagccctc
     3661 gggcgagggt tgggtcgagt acgagacgtg cggccatgtc ggacgcgatc gtgacaggaa
     3721 aggaaccttc ctgctatccg cgattgacgc tcatggcccc tcccgtgtcc tcgacctcgg
     3781 cgccaacgac gcatacttct ccgagatggc cacggccaac ggagcgctcg ccatcgcagt
     3841 cgacggcgac gaggaggtgc tcgacgccgt ctatcggcgg ggctccggtg tctcgatcgt
     3901 gctcggggac ctgaccaatc cctcgccgtc acagggttgg gccggcatgg aacgaccctc
     3961 gctcgacgag cgtgcgagac ccgacctgtt agtcgcatac gggttgatcc atcacctcat
     4021 ctacacagcg agcatcccgc cacgatccgt gatcgactgg ctcgcctctt acgagtgccc
     4081 tgtcgtcttg gagttcgtcg acccgtccga tgagatggtg gccaagctca cggccaacaa
     4141 gaccgaggag gagcttcacc cggggcgaac caggtacgag ttcgagtcga tcgtggccac
     4201 ccgattcagg acactcgaca tgatggagct cggcggcggc gtc
//
LOCUS       DASUTA010000414         2998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_640956, whole genome shotgun sequence.
ACCESSION   DASUTA010000414 DASUTA010000000
VERSION     DASUTA010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640956"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VFZ80_08200"
     CDS             complement(<1..799)
                     /locus_tag="VFZ80_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287453.1"
                     /translation="MLPVITGFIDELRDAGVPVSMVEAIDAMRAVEAIDMGSRVALRE
                     TLRATLVKNLRHERAFDTAFDIYFATVPAPTDEESSDSDGPGPSLSGEGQGGPGESAF
                     DEDQLFQALLNALANMDSEAIRRGVRDAVDQLAGMEPGRPVGGTYYLYRTLRRLDMAK
                     LEAELIEALTGDQELSEFEERLLREEIESLLERLREEIQEEIRRRLVADRGREAVAKT
                     LRRPLIEDVDLMHATSNDLAEMEAAIGPLTRKLAARLARRRKKRTGRL"
     gene            complement(799..1662)
                     /locus_tag="VFZ80_08205"
     CDS             complement(799..1662)
                     /locus_tag="VFZ80_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX6287454.1"
                     /translation="MQFDGPGDVLEALRTVDYLADDRIAQVVFLASRLNKPVLVEGPA
                     GVGKTELAKAMAAVSGRKLIRLQCYEGLDEAKALYEWNYKKQLLRIQSDVDHDWDTIS
                     HDIFSEEFLLTRPLLEAIRSTEPVVLLIDEVDRVEVETEALLLEILSDFQVSIPELGT
                     MSANTQPMVFLTSNNTRELSEALKRRCLFLHIDYPDTEREQAILNARVPGLSEHLAAQ
                     VAKVARSIRSLDLKKSPSVSETIDWARTLIALSVEDVDESVLGDTLHVLLKYQTDIEK
                     AAKELTSSPQS"
     gene            1782..>2998
                     /gene="trkA"
                     /locus_tag="VFZ80_08210"
     CDS             1782..>2998
                     /gene="trkA"
                     /locus_tag="VFZ80_08210"
                     /inference="COORDINATES: protein motif:HMM:NF007039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEX6287455.1"
                     /translation="MRIVIVGAGAVGSYLAERLSSGGQDVIVIEDDENRASELQESID
                     ALVLHGNGASVHMLEQARAGDTDLLIAVTSSDGANILACYTGTQLGAKTTIARIEDSE
                     LREGADRLGVDMIIDPSATAAEELVGIVGLGGASELIHFADGRLVMVGARVAPNAPIT
                     KGPLRQLRVREAEWGWVVAALVRDGRTIVAHGDTVVREGDHALVMTIEERVDKATKLI
                     GVKRKHIDRAIIVGGTRLAEISAEVLADAGLSVVLVDESADRCIYLATQHPRALVICG
                     DPTDPEVLGELDLGPKDAMLGLTGWDEVNLLSCLVGKASGAGMAISRFNRIAYVSLLS
                     GLGIDAAVSARLMAASAILRFVRQGQVEQVATFSDTDAEAIEIEVADGSQAVDKSLLD
                     LDLPIGVIIGGISR"
BASE COUNT          557 a          927 c          941 g          573 t
ORIGIN      
        1 ccagacgacc tgttctcttc ttcctacgcc gggccaggcg ggccgccagc ttccgcgtca
       61 gtggtccaat ggcagcctcc atttcggcca gatcgttcga ggtggcatgc attaggtcga
      121 cgtcctcgat cagggggcgt ctcagtgtct tggccaccgc ctcgcggcca cggtcggcca
      181 ccaggcgacg tctgatctcc tcctggatct cttcgcgaag ccgctccagc agactctcga
      241 tctcttcacg aagcagacgt tcctcgaact ctgaaagctc ctgatcaccg gtcaatgcct
      301 cgatcagctc agcctcgagc ttggccatgt cgagacggcg caacgtgcgg tagaggtagt
      361 aggtcccccc gacggggcgg ccgggctcca ttccggcgag ctgatccacg gcgtctctga
      421 caccccggcg tattgcctcg gagtccatgt tggccaacgc attcagcaac gcctgaaaga
      481 gctggtcctc atcgaaagcc gactcaccag gtccaccttg cccctcaccg ctcaacgaag
      541 gcccgggccc gtcgctgtca ctgctctctt cgtcggtcgg tgcgggaacg gtggcgaagt
      601 agatatcgaa agcagtgtcg aaggcgcgct catggcgcag attcttcaca agggtggctc
      661 gcagagtctc ccgcaatgcc actcgagagc ccatgtcgat ggcttcgacg gcgcgcattg
      721 cgtcgatcgc ctcgaccatc gaaaccggca cgccggcatc gcgtagctcg tcgatgaagc
      781 cggtgatgac cggaagcatc agctttgagg agaggaggtc agctctttgg cggccttctc
      841 gatgtccgtc tgatacttga gaagcacatg cagggtgtct ccgagcaccg actcgtcgac
      901 atcctcgacc gacagggcta tgagcgtgcg tgcccagtcg atggtctccg atacggaggg
      961 ggacttcttg agatcgaggc ttcgtatcga acgggccacc ttggccactt gagcagcgag
     1021 gtgctctgac agtccgggaa ccctggcgtt gaggattgcc tgctctcgct ctgtgtcggg
     1081 gtagtcgatg tggagaaaga gacagcgcct tttgagcgcc tcgctcagct cccgggtgtt
     1141 gttcgacgtg aggaagacca tcggctgagt gttggcactc atcgtcccca gctcggggat
     1201 ggacacctga aaatcggaca ggatctccag gagcaaggcc tcggtctcaa cctcgaccct
     1261 atcgacctcg tcgataagga gcaccaccgg ctcggtggag cgaatcgcct ccagaagcgg
     1321 cctggtcaac aggaactcct cagagaagat gtcatgactg atggtgtccc agtcgtggtc
     1381 gacatcagac tggattctca gcagctgctt cttgtagttc cactcgtaca gggcctttgc
     1441 ctcgtccagg ccctcgtaac actggagcct gatcagcttg cggccactca ccgcagccat
     1501 tgccttggcc agttcggttt tgccgacacc ggctgggccc tcgaccagca ccggcttgtt
     1561 cagacgcgac gctaagaaga cgacctgggc gatccggtcg tctgcgaggt aatcgactgt
     1621 gcgcaacgcc tccaagacgt cgccgggacc atcgaattgc atctagctcc agggtaagcc
     1681 ggacaagtgt aagtgggccg cactctgatg ccgaagcgac gtcgtcagag aactcccggg
     1741 gtatagcctg gatgtcctgt gcgggaacca gtggcggctc aatgcgaata gtcatagtcg
     1801 gtgccggggc ggtcgggtcc tacctggccg agcgactctc ctcgggtggc caggacgtga
     1861 tcgtcatcga agacgacgag aacagggcct cggagcttca ggaatcgatc gacgccctgg
     1921 ttctgcatgg caacggggcg agtgtccaca tgttggagca ggccagggcc ggtgacaccg
     1981 acctgttgat tgcggtcacc agctcagacg gcgcaaacat cctggcttgc tacacgggca
     2041 cccagctcgg ggccaagacg acgatcgccc gcatcgagga cagtgagctg agagaaggtg
     2101 cagaccgcct cggcgtcgac atgatcatcg acccctcggc gaccgccgcc gaagagctcg
     2161 tcggaatcgt tggccttggt ggggccagcg agttgattca tttcgccgac ggcagactgg
     2221 tgatggtggg ggcgagagtc gccccgaatg cgccaattac caaggggccc ctgagacaac
     2281 ttcgggtgag ggaggcggag tggggctggg tggtagccgc cctcgtcaga gatggccgca
     2341 ccatcgtggc ccacggggac actgtggttc gcgaaggcga tcacgctctc gtcatgacca
     2401 tcgaggagag agtcgataag gcgaccaagc tcatcggggt caagagaaag cacatcgatc
     2461 gagcgataat cgtcggcggc actcggttgg ccgagatctc ggccgaggtg ctggccgatg
     2521 cgggtctctc cgtggttctc gtcgacgagt ctgcggatcg ctgtatctac ttggcgaccc
     2581 agcacccccg cgctctggtg atatgcggcg atccgaccga tcccgaggta ctcggtgagc
     2641 tcgacctcgg ccccaaggac gcgatgctcg ggctcaccgg atgggacgag gtcaatctgc
     2701 tctcctgcct cgtcggcaag gcaagcgggg ccggcatggc gatctcgcgc ttcaatcgca
     2761 tcgcctacgt gagtctgctc agcggtcttg gcatcgacgc cgcggtctct gcccgactga
     2821 tggcagcgtc ggcgatcctt cgcttcgtac gtcagggcca ggtggaacag gtcgccacgt
     2881 tctcagacac ggacgccgaa gccattgaga tcgaggtcgc cgacggctcc caggccgtgg
     2941 acaagtcact gctcgacctc gatctaccca tcggagtcat catcggcggc atctcccg
//
LOCUS       DASUTA010000415         5340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1288223, whole genome shotgun sequence.
ACCESSION   DASUTA010000415 DASUTA010000000
VERSION     DASUTA010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288223"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VFZ80_08215"
     CDS             <1..571
                     /locus_tag="VFZ80_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6287456.1"
                     /translation="TAVLATFLLRSLPGPLQLTGLVIGFGGILAIALPSAGQGTTEAI
                     GVALVVTATVGYAVSLNMVPPLQQKYGSLPLTARILWLATPMVLPFGLIGVGQSTFAW
                     PSLLAVGAVGILGTGLAFIFMGNLAGSVGATRASFLTYLIPVVALVLGVVFRDETIAP
                     VAILGVALVIAGAILASRRETGFLRRSSP"
     gene            complement(530..1453)
                     /locus_tag="VFZ80_08220"
     CDS             complement(530..1453)
                     /locus_tag="VFZ80_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HEX6287457.1"
                     /translation="MRFTVEAWDSDYGAPTDAELEDASQNVDVTVELRPEDWRPLLPE
                     VDPVTDVLFVDGVRRVDATLWIDQPPDFPGYALAATYAAGAVRCNGTAHITETQVERG
                     LFTSAPATDVVSAVGTYAVKATKGTTPEELWLGIQQRMGDLEAAVTRSAGGGELLVVD
                     GPLSHARDIENAVGYIKTQKVQYLPLELRQVLTSLPAGYRTPLFLTTTSWSRFSWYVR
                     LANHAGPAGGLVRCEIDANCSPADAARIATRVTATLPRYASARHKDPRAPQNLYPIGG
                     LERELRHRLGDKEMAMRALRRASAEEARLSA"
     gene            complement(1450..3816)
                     /locus_tag="VFZ80_08225"
     CDS             complement(1450..3816)
                     /locus_tag="VFZ80_08225"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEX6287458.1"
                     /translation="MRPVRVEVEGFSAYRESVEVDFEGVDFFSITGPTGSGKSSLIDA
                     MIFALYGRVPRLGGNAVAPAISAGADRARVRLDFELGGTVYTAVRLASRTPSGGATVK
                     EARLQEGEQVLADGADNVTDAVESLLRLRFDDFTRTVVLPQGDFARFLTATKSERQGL
                     LRNLLGLDVYTAMRELARVRGAVASDRADSASRALEALDVPDDSTARAAIERRDTLAG
                     LSETIAEHEKMLATLDQAVSAAQSDADKLANAVERLRSITPPDRLEELDSMLVEARAG
                     LEEAEEAVEDSRKSVLELETSVSDLPSREQLSSWKLTRDRLSELEQRLEDGPLIEARQ
                     VLDTRLRTLEEARAGLEEMRERLTSARHQHAAHELAGALVAGEPCPVCTRVVEQVPAT
                     RALPEMIDLEGELLEHEANVSSAQEEVDGARSAFAKLEAARAELERQRESILSDLADV
                     PDPTETLVLQTELAAKTEELQRAKEDLAGREEAARKARSLLEDLSETSRRIGRRLTEA
                     QLSLAAAELEPPLSESDDAVVQWKELLAWQSERLDELGPALECARALIGEREAAATAS
                     RNDLIASLQAHQIPVVEPFAVQVATALQTARSTVEAHEKARNDSVELAKKVKDAKTDA
                     DVAAALANHLRANGFEQWLMAGALTELVDGANDLLGQLSGGGYSLHSDESGTFSIVDH
                     RNADEMRSVNTLSGGETFLVSLALALSLAETLAAKGGSGLDAIILDEGFGTLDDESLD
                     TVASVLEELTGRGLMVGVITHVKELAARAPNRYEVVRGATGASVKVVA"
     gene            complement(3813..4940)
                     /locus_tag="VFZ80_08230"
     CDS             complement(3813..4940)
                     /locus_tag="VFZ80_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HEX6287459.1"
                     /translation="MKILHTSDWHVGRRIRGRDRSEEHRAVLAELVEMAGRHNVDLTV
                     VAGDVFDTASPTPTSEEIVWRALLDLSEIARVLVVAGNHDNPGRLDAVAPLLERAGVT
                     VVGVPRSPEHGGVVHMPDLGVKVGLLPFVSQRGIVRAEEIMSSDPDEHAGQYEERMRR
                     VVEALTDGMTTETVNLLVSHLTVYGALAGGGERTAHVFGYAVPSSLFPGHLSYVALGH
                     LHRQQKMPHSSAVWYAGSPLQLDFGEVNDQKGALLVKAEPGRPASVEELPLTSGRRLI
                     SIKGSLGQVLSRADEVGDAYVRVVLQEAARVGLADEVRAAIPDAVEVVLESPDQGRAH
                     EPHSRQGLDPVEAFNRYLADREATDPRVESLFAELLDEAQV"
     gene            complement(5194..>5340)
                     /locus_tag="VFZ80_08235"
     CDS             complement(5194..>5340)
                     /locus_tag="VFZ80_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014279225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEX6287460.1"
                     /translation="GEFFIVPSGVEHCPSAEGECEILLLEPIGVVNTGDVQDSDLTAP
                     EEWI"
BASE COUNT          897 a         1758 c         1712 g          973 t
ORIGIN      
        1 caccgcagtt ctcgccacgt ttctgctccg atcactacca ggcccgcttc agctgaccgg
       61 cctcgtcatc ggtttcggcg ggatcctggc gatcgccctt ccatccgccg gtcaggggac
      121 cacagaggcc attggcgtcg ccctggtggt gacggccacc gtcggatatg ccgtctcact
      181 caacatggtc cccccgctcc agcagaagta cggatcgctg cctctcacgg cccgaatcct
      241 ctggctggca acgcccatgg tcctcccctt cggtctgatc ggcgtggggc aatccacgtt
      301 cgcctggccg tcgctgctgg cggtgggggc agtgggaatc ctcggaaccg gcctcgcctt
      361 catcttcatg gggaacctcg ccggcagcgt cggagcgacc cgggcctcgt tcttgacgta
      421 cctgatcccg gtcgtcgctt tggtcctggg ggtcgtgttt cgcgatgaga ccatcgcacc
      481 ggtggccatc ctcggagtcg ccctggtgat cgcaggagcg atcctggctt cacgccgaga
      541 gacgggcttc ctccgcagaa gctcgcctta gggcgcgcat cgccatctcc ttgtcgccca
      601 gccggtggcg gagctctcgt tcgaggccac ctatcggata gagattctgc ggggcacgcg
      661 ggtctttgtg ccgggccgag gcatatcgag gaagcgtggc agtgacccgg gtagcgatgc
      721 gtgccgcatc ggcagggctg caattggcgt cgatctcaca tcgaaccagg ccgcccgccg
      781 gtcccgcgtg gttggcgaga cgcacgtacc acgagaaccg gctccatgag gtggtggtga
      841 ggaacagtgg ggtcctgtat ccggccggca acgaggtgag cacttgccgt aactcgagcg
      901 gcaagtactg gaccttctgg gtcttgatat agcccaccgc gttctcgatg tcccgagcgt
      961 gggagagagg gccgtcgacc acgagaagct cgccccctcc tgcactccgg gtcaccgctg
     1021 cttccaggtc tcccatccgc tgttggatgc ccaaccacag ctcctcgggt gtcgtcccct
     1081 ttgtggcttt caccgcgtag gtgccgacag cgctcaccac gtcggtcgcc ggggccgacg
     1141 tgaacaagcc ccgctccact tgggtctcgg tgatgtgggc ggtgccattg cagcggaccg
     1201 ctccggcggc ataggtggcg gccagcgcgt aacccgggaa gtcgggcggc tgatcgatcc
     1261 agagtgtggc atcgacacga cggaccccat cgacgaatag gacgtcggtg accggatcca
     1321 cctcgggcaa gagaggacgc cagtcctcgg gcctcagttc gactgtcacg tctacgtttt
     1381 ggctggcgtc ctcgagctcg gcatcggtgg gtgccccgta gtcggagtcc caagcctcga
     1441 cggtgaacct catgccacga ccttcacgct ggcgcccgtc gccccgcgga cgacctcgta
     1501 tcgattgggc gcccgggccg ccagctcctt gacatgtgtg atgaccccga ccatgagccc
     1561 acgtccggtc agctcttcga ggacggaggc gactgtgtcg agcgactcgt cgtcgagagt
     1621 gccgaatccc tcgtcgagga tgatggcgtc cagccccgac cctcccttgg cggccagggt
     1681 ctcggcgagg gacagggcaa gggccaacga gacgaggaag gtctcaccac ccgagagggt
     1741 gttgacagat cgcatctcgt ctgcgttgcg atggtcgact atcgagaagg tgcccgactc
     1801 gtctgagtga agcgaatagc cgccgcccga cagctgaccc agcagatcgt tggctccgtc
     1861 gaccagctcg gtgagcgctc ccgccatgag ccattgctcg aagccgttgg cgcggaggtg
     1921 gttggccaga gccgccgcca cgtcggcgtc ggtctttgca tccttcacct tcttcgccag
     1981 ctccaccgaa tcgtttcggg ccttctcgtg ggcctcgacg gtgctacgcg ccgtctgcaa
     2041 cgccgtggcc acctgaactg cgaagggctc gactaccgga atctggtgcg cttgaagact
     2101 ggcgatcaag tcgttgcggg aagccgtggc agctgcctct cgttcgccga tgagcgcccg
     2161 ggcgcattcg agtgcggggc caagctcatc gagacgttcc gattgccatg cgaggagctc
     2221 tttccactga accacggcgt catcgctctc ggagagaggc ggctccagtt cggcagctgc
     2281 cagcgaaagc tgggcttcgg tgagtctgcg gccgatacgc cgcgaggtct cggaaagatc
     2341 ctcgaggagc gagcgcgctt tgcgcgccgc ctcctcgcgt ccggcgagat cctctttcgc
     2401 tcgctgcagt tcctcggtct tggccgcgag ttcggtctga agaaccaatg tctctgttgg
     2461 gtcagggaca tcggccagat cggacaggat cgattctcgc tgcctctcta gctctgcgcg
     2521 tgccgcttcg agcttggcga aagcggagcg agcaccatcc acttcctctt gagcagacga
     2581 gacgttggct tcatgctcca gcaactcgcc ttccaggtcg atcatttcgg gaagtgcgcg
     2641 agtcgcgggc acttgctcca cgacacgggt gcaaaccgga cacggctcac ccgccaccaa
     2701 cgctccggcc agctcgtggg cagcatgttg gtgtcgggca gaagtcagtc gttctcgcat
     2761 ctcctccaac ccggctcttg cctcctcgag agttctcagc ctggtgtcga gaacctgacg
     2821 agcctcgatc aacggcccat cttcgagacg ctgctccagc tcggagagtc tgtctcgggt
     2881 gagcttccac gagctgagct gctctctact cggcaggtcg gacaccgatg tctcgagctc
     2941 caacacggac ttgcgggagt cttcgaccgc ctcttcggct tcctcgagac cggccctggc
     3001 ctccacgagc atcgagtcga gttcttcgag ccgatcgggt ggggtgatcg atcgcagacg
     3061 ctcgaccgca ttggcgagct tgtcggcatc cgattgcgcc gccgataccg cctggtcgag
     3121 ggtggccagc atcttctcgt gttcggcgat cgtctcggac agcccggcga gggtgtcccg
     3181 ccgctcgatg gcggcccggg cggtgctgtc atcgggaaca tcgagcgcct ccagcgcacg
     3241 cgaggccgag tcggcgcggt cgctggcgac ggcccctctg actcttgcca gctcgcgcat
     3301 ggctgtgtag acgtcgaggc cgaggagatt tcggagcaga ccctgccgct ccgatttggt
     3361 tgcggtgagg aatcgtgcga agtcgccctg gggcaagacc accgtccggg tgaagtcgtc
     3421 gaacctgagc cggagcaggc tctcgacggc atcggtgacg ttgtcggcgc cgtccgccag
     3481 aacctgctcc ccctcctgga gcctggcttc tttgacggtg gcgccaccgc taggggttct
     3541 cgaggcgagg cgaacggcgg tgtacacggt gcctcccagc tcgaagtcga gcctgacccg
     3601 agcccgatcg gcccctgcgc tgatcgcagg cgccacggcg ttgccgccga gacggggaac
     3661 ccgtccgtag agggcgaaga tcatcgcgtc gatgagactc gactttcctg acccggtggg
     3721 gccggtgatg gagaagaagt cgacgccttc gaagtcgact tccaccgact ctcgataggc
     3781 cgaaaagcct tcgacctcga cgcgaaccgg tctcacacct gtgcctcgtc gagaagttcg
     3841 gcgaaaaggg actcgactct gggatcggtc gcctcgcggt cagccaggta ccggttgaat
     3901 gcctctacgg ggtcgaggcc ctggcgggaa tgcggctcgt gcgcccgacc ctgatccggg
     3961 ctctcgagga cgacctcgac agcatcggga atcgcggccc ggacctcgtc tgccagaccg
     4021 acccgcgcgg cctcttggag caccacacgg acgtaggcgt caccgacctc atcggcccgg
     4081 gacaggacct gaccgagact gcccttgatc gagatcagtc gtcggcccga ggtgaggggg
     4141 agctcctcga ccgaagcggg tcgccccggc tctgccttca ccaacaatgc ccccttctgg
     4201 tcgttgacct caccgaagtc gagctgaagt ggcgaccctg cataccaaac agcagacgag
     4261 tgcggcatct tctgctgacg atggagatga ccgagcgcca cataggagag gtggccgggg
     4321 aagagactgg agggcacggc atatccaaag acgtgagcgg tgcgctcacc tccgccggca
     4381 agtgcgccgt aaacggtgag atgactgacc aggagattca ccgtctcggt ggtcattccg
     4441 tctgtcaggg cctcgaccac ccgacgcatc cgctcctcgt attgaccggc gtgctcgtcg
     4501 ggatcggagc tcatgatctc ctcggccctg acgatccccc gttgggagac gaagggaaga
     4561 aggccgacct tcacgccgag gtccggcatg tggacgacac cgccgtgctc cggcgagcga
     4621 ggaacaccaa ccacggtgac accagcccgc tcgagaagcg gggccacggc gtcgaggcga
     4681 ccggggttgt cgtggtttcc ggcaacgacc agcactcggg cgatctcgga caggtcgagc
     4741 aaggccctcc agacgatctc ctcagaagtc ggcgtgggcg aggcggtatc gaacacgtcg
     4801 cctgcgacca ccgtcaggtc gacgttgtgc cgacccgcca tctcgacgag ctcggcgagc
     4861 acggctcgat gctcttcgga gcggtcgcgc cctcgtatcc ggcggcccac atgccagtcg
     4921 gatgtgtgca atatcttcac gtcgaagagc gtaggaatcg ggtacgacag aatcgcggag
     4981 cgtcagcgct gaatgctgag cgcagcgtct atacgcaacc ggcgccgggg ggacaaagca
     5041 cgatggtcaa gtacaggtat ccgactacga ccaagcccac gaccgcggcg ccgatgccga
     5101 ccgagacagc cagagcccta ctttgcgttc ggccccagca agcgtcactc acctgctggc
     5161 gcagtgaacc gttgatctgt tctgcaagcc gggctagatc cattcctctg gcgcagtgag
     5221 gtcactgtcc tggacgtcac cggtattgac cacgccaatt ggctcgagga gcagaatctc
     5281 gcactcgcct tctgcactgg gacagtgctc cacgccgctc ggcacgatga agaactcgcc
//
LOCUS       DASUTA010000416         9781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1159919, whole genome shotgun sequence.
ACCESSION   DASUTA010000416 DASUTA010000000
VERSION     DASUTA010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 9781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9781
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159919"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFZ80_08240"
     CDS             complement(<1..230)
                     /locus_tag="VFZ80_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6287461.1"
                     /translation="MDAEAWDERYRASELLWSAGPNIWVERRLADAEPGRGLDLASGE
                     GRNAIWLAKRGWEMTAVDFSAVAIERGRSLSD"
     gene            complement(232..1746)
                     /locus_tag="VFZ80_08245"
     CDS             complement(232..1746)
                     /locus_tag="VFZ80_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6287462.1"
                     /translation="MNAQDVLLKLGIESENSGVFAGEWLEATGETIDVVNPTSGATIA
                     RVTMASRDDYEKVASSSTETFERWRLLPAPKRGEYVRQIGNALRENLDALGALVTLEM
                     GKIHLEGVGEVQEMIDICDFAVGLSRQLYGNTMASERPNHRMYEHWHPLGPVGVITAF
                     NFPVAVWSWNAAIAAVCGDTVVWKPSEVTPLTAIAVTRIAQGVMDGSGFEGVFNLAVG
                     DGKTVGEAMTADPRLPLISATGSCAMGRIVGPAVAKRMGRTILELGGNNAVIVMDDAD
                     LDLAVRAALFAAVGTAGQRCTTLRRLLVHRAVSDKVVNRLAEAYRQVSIGDPLEKGTL
                     MGPVVSERAIDAMMSSIEIARDQGGEVLTGGRRVDREGYFVEPTIVKVPKEASITQDE
                     TFAPILWVIEFDTLDEAIEIQNGVTQGLSSAIFTESLKNAERFLSATGSDCGIANVNI
                     GTSGAEIGGAFGGEKETGGGREAGSDAWKAYMRRQTTTINWGDDLPLAQGIEFG"
     gene            complement(1743..2123)
                     /locus_tag="VFZ80_08250"
     CDS             complement(1743..2123)
                     /locus_tag="VFZ80_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006235050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEX6287463.1"
                     /translation="MPKTVPETPSAPESLAPYSAATEAGGLVFLSGQVGLDPISGERA
                     PDDVTAQAHQVMRNIGGVLGDLGLGYEDIVKTTVFLADISDFGDVNEVYKSYFDGPFP
                     ARSAVQAGALPGGFLVEIEVVAAR"
     gene            complement(2245..2607)
                     /gene="erpA"
                     /locus_tag="VFZ80_08255"
     CDS             complement(2245..2607)
                     /gene="erpA"
                     /locus_tag="VFZ80_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020020116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX6287464.1"
                     /translation="MTQILSIKSSQPQKTVNLTDSAVAKVRELIAGEDEAGLALRMAV
                     RPGGCSGFSYEMYFDSEVDDSDIIEEFEDVKVLVDPQSAEMVRGATLDYKEGLMGAGF
                     AIDNPNVTRSCGCGNSFS"
     gene            2779..3897
                     /locus_tag="VFZ80_08260"
     CDS             2779..3897
                     /locus_tag="VFZ80_08260"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX6287465.1"
                     /translation="MSSVTKDQVIKALEGVRDPELGGNVVELGMITDVSIEDDHVEIG
                     LALTIAECPLRSQIEGDTRRRVESMPGVESVGIRTTAMTKRQRAELMSVARRQARENA
                     EPTQVGSTTRVIAVASGKGGVGKSSLSVNLAAGLAQEGFDVGLLDADIWGYSIPRMIG
                     AAGRLEADAESKLIEPVTANGVRVVSTGLIIDSEDTALMWRGMMLSKALEQFLKQVRW
                     GDLDYLIIDMPPGTGDVQMALTRMLPQAEMIVITTPQSAAQRVAIRVADMARRSHMPV
                     VGVIENMSGDVFGEGGGKVLADELGVSLLGSIPLDQSVVEGGDSGRPVVLEQGSGPAA
                     SALAGTVERVIAVVPPADMETCTGRIAKLIADLEDGAA"
     gene            complement(3913..4389)
                     /locus_tag="VFZ80_08265"
     CDS             complement(3913..4389)
                     /locus_tag="VFZ80_08265"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX6287466.1"
                     /translation="MTTPEPPSAVPQPTSVPGPLADWGTRAIGFLIDYLPIILLNALT
                     FWSGGLRYIAGLLGLAYWIYMGYLDGVAGQTPGKAMQGIQLVNAQGGLIGSGAGIGRK
                     FLHILDAICFIGFLLPIVDAQRQTFADKLMTTYVVTGAEKKPFAIELWTSTPQQST"
     gene            complement(4643..5821)
                     /locus_tag="VFZ80_08270"
     CDS             complement(4643..5821)
                     /locus_tag="VFZ80_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX6287467.1"
                     /translation="MKRLTRLLSGLLVVAFAVPALGQVTDDDIDRAREEVNRITEESA
                     DVGQQVIDAYGRQAALDQEITDLRESIDFARLKIAETETKLEELAVELYMGSTSGASL
                     SVLFSATDQEYPAGMEYLRNVSGVNEDVVGQLRTFRDELDRQTHRLDDALEEQVVLAG
                     ELETLAEQLQDDLVAAQVVYDDLIRQQAEEEAERRRQEEERQRQEEAARQAAAQEAAN
                     ATTTTASPPATTAGGSDSGAATTTTAAVTATTASPPPSLPSGSGTCPVAGAVSFTDSW
                     GDPRSGGRTHKGVDMIAARGTPIVAIYSGRVYRLSNSSLGGLSVYFYSDAGDLYYYAH
                     LDSFGDISPGQSVPVGHVLGYNGSSGNAPDYLPHLHFEYHPGGGSAVNPYPLVKGLCG
                     "
     gene            complement(5818..6591)
                     /locus_tag="VFZ80_08275"
     CDS             complement(5818..6591)
                     /locus_tag="VFZ80_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6287468.1"
                     /translation="MRIDRILANNPGPFTGPGTNTWILDDGNGFVAVIDPGPVDSKHA
                     RSILESVGTRALVAVLVTHTHSDHAPLANPIARDMGVPAVGYAPGPSFQPDIRLLEGA
                     RFGVGDLDLEVIHTPGHSKDHLCFRVRNVLFTGDHIMGGSSVMVEEMGPYLRSLEKLR
                     GTGLERLHPGHGDEMGDPDGVIDWYLAHRLQRHEQIREAIEGGAGTIPEIVEMVYADV
                     DSSLHPLAARSVQAHVTLLQDEERIALRGDRLVAAPSPQ"
     gene            complement(6588..7256)
                     /locus_tag="VFZ80_08280"
     CDS             complement(6588..7256)
                     /locus_tag="VFZ80_08280"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX6287469.1"
                     /translation="MPSPDPAASVLLLRDSGHGFQVLMVERNARGFFADLLVFPGGGV
                     DECDVPEGRGRWDEISHRRAAVRELAEETGILISGGTSRVAPLAKGPGFYQEVEEHEM
                     EVATGALTLVSRWVTPELAPRRFDTRFYLATCGDSPRVTLDTEELVAYDWVDPADSVS
                     RYEAGEMNLILPTLAHLRWLSRRSSIEDALESASGADGRTLIRPERMEDGSILPVHLP
                     AEVI"
     gene            7296..8618
                     /gene="selA"
                     /locus_tag="VFZ80_08285"
     CDS             7296..8618
                     /gene="selA"
                     /locus_tag="VFZ80_08285"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX6287470.1"
                     /translation="MADNPYRALPSVDVLADEVVSSLPRAVVVDIARLTLEQARGEIA
                     TGDDPDVVAQANRVAHAVERSAGTGVINATGVLLHTNLGRALWSDSAAHRAFEAATSF
                     SNLEMDLETGARSRRGSYVTRLLQHLTNAEDALVVNNNAAALLLALAATARGRAVPVS
                     RGELIEIGGSYRLPSVMEVSGARLVEVGTTNRTRLDDYRTAIQTYQCGALLKVHPSNY
                     RIEGFTEESSLTELATLSTDEVPLLYDLGSGLLDEGAPWVPDWLRSEPGVRQSLAAGA
                     DLVMFSGDKLLGGPQAGVLVGDADLIERLRSNPLTRALRVDSVTYAGLGATLEVYLGA
                     DPTAIPFWRHALTPVDSLESRLESFSSVTGGEVVEAASVVGAGSAPGLSIPTPVLRIQ
                     DGASMFGGLISQNRPVLTRRDKGDLIVDLRAVDPQDDEVVVTALERCR"
     gene            8609..>9781
                     /gene="selB"
                     /locus_tag="VFZ80_08290"
     CDS             8609..>9781
                     /gene="selB"
                     /locus_tag="VFZ80_08290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEX6287471.1"
                     /translation="MPVIGTAGHVDHGKSTLIQRITGRNPDRWAEERERGLTIDLGFA
                     WTTLPNGIEVSFVDVPGHERFLKNMLAGIETIDFALFVVAADEGWMPQSEEHLAVLDL
                     LEVTHGVIALTKSDAVEPDLLDLATVETEERLEGTSLAEAPIMAVSGTTGDGVPDLLK
                     TISEHLPEPPASTGRPRLWVDRAFAVSGAGTVATGTLLDGSLQVDDGVMVYPSGRASR
                     VRGIQSHEKSLDQVEPARRVALNLAGLEHQDLARGDMIGFPGQWVVSDRFAVSLRTAR
                     FVEEIDQRGAYQLHIGSSAHRVVITGLENNGAVLQLDAAIPVAVGDRFILRDTGRRLA
                     VAGGRVLDPSPIKTRMAIKASSRIDPSASPDQIADTLLTIRGRERVQRLAAESRGGS"
BASE COUNT         1788 a         3160 c         3108 g         1725 t
ORIGIN      
        1 tggtcggaga gactccggcc ccgctcgatt gccacagcgg agaaatcgac tgccgtcatc
       61 tcccacccgc gtttggccag ccagatcgca ttgcgtccct cacccgacgc caggtcgagg
      121 ccccggcccg gttcggcgtc ggcaagccgt ctctcgaccc agatgttggg cccagcagac
      181 cagaggagct cggacgcccg atagcgctcg tcccaagcct cggcgtccac gtcagccgaa
      241 ctcgatgccc tgagccaacg gcagatcgtc gccccagttg atggtcgtcg tctgacgacg
      301 catgtatgcc ttccaggcgt cggagcccgc ctcgcgtccg ccgcccgtct ccttctcccc
      361 cccaaaggct ccgccgatct cggctcccga tgtgccgatg ttgacgttgg cgatgccgca
      421 atccgatccg gtggcgctga ggaatcgttc cgcgttcttg agcgactcgg tgaagatcgc
      481 cgacgagagg ccctgggtca ccccgttctg gatctcgatc gcctcgtcga gggtgtcgaa
      541 ctcgatcacc cagagtatgg gggcaaaggt ctcgtcctgt gttatcgagg cttccttggg
      601 aaccttcacg atcgtgggct cgacgaagta gccctcgcga tccactcgcc ttccgccggt
      661 caatacctct ccgccctgat ctctggcgat ctcgatcgag ctcatcatgg cgtcgatggc
      721 gcgctcggac acgaccgggc ccatgagcgt ccccttctcg aggggatcgc cgatgctcac
      781 ctgccgatag gcctcggcta gacgattgac caccttgtcg gagacagcac gatggacgag
      841 aagtcgacgc agcgtggtgc accgctgtcc tgcggtgccc accgcggcga acagggcagc
      901 gcgcaccgcg aggtcgaggt cggcgtcgtc catgacgatg actgcgttgt tcccccctag
      961 ttcgagaatg gtccgaccca tccgcttggc caccgccgga cccactatcc gacccatggc
     1021 gcaggagccg gtggccgaga tcagtggaag acggggatcg gcggtcatcg cctcaccgac
     1081 ggtcttgcca tcaccgacgg ccagattgaa cacgccctcg aaccccgagc cgtccatgac
     1141 accctgagcg atcctggtga cggcgatggc ggtgagcggg gtgacctcgg agggcttcca
     1201 gaccacggtg tcgccacaga ccgcggcgat ggcggcattc cacgaccaga cggcgaccgg
     1261 gaagttgaaa gcggtgatga ctccgaccgg gcccagagga tgccagtgct cgtacatcct
     1321 gtggttgggc cgttctgagg ccatggtgtt cccatagagc tgacgggaga gcccgaccgc
     1381 gaagtcgcag atgtcgatca tctcctgaac ctcgccgaca ccctcgagat ggatcttccc
     1441 catctccagg gtcaccagcg caccgagggc atcgagattc tcacgcaagg cgttgccgat
     1501 ctgccggacg tactcgccgc gcttcggcgc cggcagaagg cgccagcgct cgaatgtctc
     1561 ggtcgaagac gaagccacct tttcgtagtc gtcacgcgat gccatggtga ccctggcaat
     1621 ggtcgccccg gatgtcgggt tcacgacgtc gatggtctcc ccggtggcct ccagccactc
     1681 cccggcaaac acccccgagt tttcagactc gatcccgagc ttcagtaaca cgtcctgtgc
     1741 gttcatcgtg ctgccaccac ctcgatctcg accaggaaac cgccaggcag agctccagcc
     1801 tgcaccgcgg accgagcagg aaagggcccg tcgaagtacg acttgtagac ctcgttgacg
     1861 tcgccgaagt cggagatgtc ggcgaggaag acggtggtct tgacgatgtc ctcgtatccg
     1921 agcccgagat caccgagcac gcctccgatg ttgcgcatca cctgatgtgc ttgcgccgtg
     1981 acgtcgtccg gtgcccgctc accgctgatc gggtccaacc cgacctggcc ggataggaag
     2041 acgagtccgc cggcctccgt ggccgcggaa tacggcgcca atgactcggg ggcggatggg
     2101 gtctcgggaa cggtcttggg caagttctca gatctcctgt cgattcattg cgtgagcgta
     2161 cccaaagaag gaagggaccc ggccggagcc gggtcccttc gcattgcaag ccactgaggg
     2221 gacgcttgcc cccggggcaa gttctcagct gaaagagttg ccgcagccac agctgcgggt
     2281 gacatttggg ttgtcgatgg caaacccggc tcccatcaga ccctccttgt agtcgagggt
     2341 ggcgccgcgc accatctcgg cactctgggg atcgaccaga accttgacgt cctcgaactc
     2401 ctcgatgatg tcggagtcgt cgacctcgga gtcgaagtac atctcgtagg agaatccgga
     2461 gcagccaccg ggacggacgg ccatccgcaa cgcaagcccg gcctcgtctt cgccggcgat
     2521 cagctcacgg accttggcca cggccgaatc ggtgagattg accgttttct gcggttgcga
     2581 tgacttgatg gaaagtatct gtgtcattgg ttcctacctg tgctctcgat cattgtaacg
     2641 acacgacggg gatcagccat tcccgtgcac cagaccgttt ctcttgaggc gtgctgggca
     2701 cagaccccgt tctgtcttca cagagcggca tatcagcgac atttagctac tcgcgtagtg
     2761 caaattaccc ttgatgtcat gtcctccgtg acgaaggacc aggtcatcaa ggccctcgaa
     2821 ggggtcaggg acccggagtt gggcggcaac gtcgtcgagc tcgggatgat caccgacgtc
     2881 tcgatagagg atgatcacgt cgagatcggt ctggcactga caatcgcgga atgccctcta
     2941 cgtagccaga tcgaaggtga caccagacgc cgggtcgagt cgatgccagg cgtcgagagc
     3001 gtgggcatcc ggacgacggc gatgaccaaa cgccagcgtg ccgagctgat gtcggtggcg
     3061 cggaggcagg cacgtgaaaa tgccgaaccg acccaggtcg gctcgaccac gcgggtgata
     3121 gcagtcgcat cggggaaggg tggtgtcggg aagtcgtcgc tctcggtgaa cctggcggcg
     3181 gggctcgccc aggaggggtt cgacgtcggt ctgctcgacg ccgacatctg gggatactcg
     3241 attccccgga tgatcggtgc agccggtcgg ctcgaagccg acgctgagag caagctgatc
     3301 gagccggtca ctgccaatgg ggtcagggtg gtctcaaccg gcctcatcat cgactcggag
     3361 gacaccgcgc tcatgtggcg cgggatgatg ttgtcgaagg ccctcgagca attcttgaag
     3421 caggtgcgat ggggggacct cgactatctg atcatcgaca tgcccccagg caccggagat
     3481 gtgcagatgg cgctcacccg catgctgcct caggcagaga tgatcgtgat caccaccccg
     3541 cagagtgcgg cgcaacgagt ggccatcaga gtcgccgaca tggcgcggcg ctctcacatg
     3601 ccggtggtcg gagtcatcga gaacatgtct ggggacgtct tcggcgaggg cggcggcaaa
     3661 gtgctcgccg acgagttggg ggtctccctc ctcgggtcga tcccgctcga tcagtctgtg
     3721 gtcgaagggg gagacagcgg caggccggtg gtgctggagc aaggctcagg cccggcagcg
     3781 agcgccctgg cgggcacggt ggaaagggtc attgcggtgg tgcctccagc cgacatggag
     3841 acctgtaccg gcaggatcgc caagctgatc gccgacctgg aggacggcgc ggcgtagacc
     3901 gttgggttcg ggctatgtgc tctgctgcgg cgtagaggtc cacaactcta tggcaaaggg
     3961 cttcttctcg gcaccggtaa ccacataggt cgtcatcaat ttgtcagcga atgtctgacg
     4021 ctgtgcatcg acgatgggga gcaggaagcc gatgaagcaa atggcatcga ggatatgcag
     4081 gaatttgcga ccgatcccgg ccccagagcc gatcaggccg ccctgagcgt tgaccagctg
     4141 tatcccttgc atggccttgc ccggagtctg tcccgcgaca ccgtcgaggt agcccatata
     4201 gatccagtag gcgagaccga ggagcccggc gatgtaacgc aagccgccgc tccagaaggt
     4261 gagcgcgttg agaaggatga tcggcagata gtcgatgagg aatccaatgg cccgtgtccc
     4321 ccagtcggcc agcggacctg gcaccgatgt cggttgcggc accgccgacg gcggctcagg
     4381 tgtggtcaag gtgagctcct actcgtgact gggcggcacg ttagacaccg acgcagtccc
     4441 cgcgccgcca gaaccgcccc tggacgcgag aaagggaccg caatcggtcc cttctcgaga
     4501 aggcctccca ggccccacac ttctcccagc gtccgccggg tgcacgttca ggcatgaata
     4561 ttggccacta ccagtggttt cgcaatgact gttcgtgaga tcgacactgt gactagtcca
     4621 cggttgtgga taactcgctc ggtcagccgc agagaccctt caccagggga taggggttga
     4681 ctgccgagcc gccaccaggg tgatactcga agtggagatg gggtaggtag tcgggggcgt
     4741 tgccactgga gccgttgtac ccgagcacat ggcccaccgg cacactctga cccgggctga
     4801 tgtcgccaaa cgagtcgagg tgcgcgtagt aatagaggtc accggcatcc gagtagaagt
     4861 acaccgagag accgccgagt gaggagttcg acaggcgata caccctgccc gagtaaatgg
     4921 cgacgatagg ggtccccctc gccgcgatca tgtcgacacc cttgtgggtc ctccctcccg
     4981 acctggggtc accccagcta tcggtgaagg acacggctcc tgccaccggg caggtgccgc
     5041 tgccagacgg gagcgatggc gggggtgacg ccgtggtggc ggtgactgca gctgtagtgg
     5101 tggttgcggc accactgtcg gagccaccgg ccgtcgttgc cgggggcgac gccgttgtgg
     5161 tagtcgcatt ggcggcctcc tgcgcggccg cctgacgggc agcctcttcc tgtcgttgcc
     5221 gctcctcttc ctgacgacgc cgctccgctt cctcttccgc ttgctgacgg attagatcgt
     5281 cgtacacgac ctgcgccgcc accagatcgt cctgaagctg ttcggcgagg gtctccagtt
     5341 cgcccgcaag cactacctgt tcctcgagcg catcgtcgag ccggtgagtc tgacggtcta
     5401 gctcatcacg aaacgtcctg agctggccaa ctacgtcttc gttcacgccg ctcacattgc
     5461 gcaggtactc catacccgcc gggtactcct ggtcggtcgc cgagaagagg accgacaatg
     5521 aggcgccgct cgtcgatccc atgtacagct cgacggccag ttcctcgagc ttcgtctcgg
     5581 tctcggcgat cttcagccgg gcgaagtcga tggactcgcg gaggtcggtg atctcctgat
     5641 cgagggcggc ctggcgcccg taggcgtcga tcacctgttg gccgacgtcg gccgactcct
     5701 ctgtgatcct gttgacctcc tcccgagcgc gatcgatgtc gtcgtcggtg acctgtccaa
     5761 gggcggggac ggcgaacgcc acgaccagca aaccgctgag gagtcgagtg agtcgtttca
     5821 ttggggggat ggggctgcaa ccagtcggtc gccccgtaag gctatccgct cttcgtcctg
     5881 aagcaacgtg acgtgggcct gcaccgacct cgccgccagg ggatgaagcg aagaatcgac
     5941 atcggcgtac accatctcga cgatttcggg aatggtccct gctccaccct cgatagcctc
     6001 gcggatctgc tcgtgacgct gcagccgatg agcgagatac caatcgatca cgccatcggg
     6061 gtcgcccatc tcgtcgccat ggccaggatg cagcctctcg agccctgtcc cccgcagctt
     6121 ctccagcgac cggaggtaag gacccatctc ctcgaccatc accgacgacc ctcccatgat
     6181 gtgatcgccg gtgaacagaa cgttgcggac cctgaaacag aggtggtcct tggaatgacc
     6241 cggggtgtgg atcacctcga ggtcgaggtc gcccaccccg aaccgggcac cttcgagcag
     6301 cctgatatcg ggctggaacg agggcccggg cgcatagccc acggcaggga cacccatatc
     6361 gcgagcgatc ggattggcca gtggggcgtg atcgctgtgg gtgtgagtga ccagcaccgc
     6421 gaccaacgcg cgagtgccca ccgactccag gatcgacctg gcgtgcttgc tgtcgacggg
     6481 ccccgggtcg atcaccgcga cgaagccgtt cccgtcgtcg aggatccacg tgttggttcc
     6541 cggcccggtg aaaggacctg gattgttggc gagtatccga tcgatcctca tatgacctcg
     6601 gccggcagat gaacggggag gatcgacccg tcctccatcc gctcggggcg tatgagggta
     6661 cgtccatcgg ccccactcgc tgactcgagg gcatcctcga tcgatgacct ccgggaaagc
     6721 caacgcaggt gggcaagggt cggcaggatc agattcatct cccctgcctc gtagcgactc
     6781 accgaatcgg cgggatctac ccagtcataa gcgaccagct cctcggtgtc gagtgtgacc
     6841 cgcggtgagt caccacaggt ggcgagatag aagcgggtgt caaagcggcg tggtgccagc
     6901 tcgggggtca cccaccgtga gaccagggtc agggcaccgg tcgccacctc catctcgtgt
     6961 tcctccacct cctggtagaa accggggcct ttcgccaggg gcgcaacccg ggaggtccca
     7021 ccgctgatca ggatgccggt ctcttcggcc aattcccgga ccgcggcccg gcgatgcgag
     7081 atctcatccc accgacctcg cccttcagga acatcgcact catcgacacc gccgccagga
     7141 aagacgagca ggtcggcgaa gaagcctcgt gcatttctct ccaccatcaa cacctggaag
     7201 ccgtggccag aatcgcgcag gagcaggaca ctggcggccg ggtcgggact gggcacgtgc
     7261 gcatcctatg gaatggagat gccaaactcg gcgtcgtggc cgacaacccg taccgagctc
     7321 tgccctcagt cgacgtcctc gctgacgagg tcgtctccag ccttccccgg gccgtcgtcg
     7381 tcgacattgc tcgactcacc ctcgaacagg cgcgaggcga gatcgccacc ggagacgatc
     7441 ccgacgtcgt tgcccaggcg aaccgagtcg cacacgcagt cgaacgttca gccggcaccg
     7501 gggtgatcaa cgccaccgga gtcctccttc acaccaacct gggacgggcc ctgtggtcgg
     7561 attcggcagc tcatcgtgca ttcgaggccg ccacctcatt cagcaacctc gagatggatc
     7621 tcgaaaccgg agcgcggagc cgaagaggtt cgtacgtgac ccgtctgctc cagcatctga
     7681 ccaacgccga ggacgccctt gtcgtcaaca acaacgctgc ggcgctcctt ctcgctctcg
     7741 ccgcgacggc gcggggaaga gccgtcccgg tctctcgggg tgagctcatc gagatcggag
     7801 gctcataccg gctcccgtcg gtcatggagg tgagcggcgc ccgactggtg gaggttggca
     7861 ccacgaaccg aacccggctc gatgactacc ggactgccat ccagacctac cagtgcggcg
     7921 ccctgctcaa ggtccatcca tcgaactacc ggatcgaagg tttcaccgaa gagtccagcc
     7981 tgacggagct cgccacactg agcactgatg aggtcccgct gctctacgac ctgggttcgg
     8041 ggctgctgga tgagggtgcc ccctgggtgc ccgactggtt gcgctccgag cccggggtca
     8101 ggcagtctct cgccgccggc gcggacttgg tgatgttcag tggcgacaag ctgctgggcg
     8161 ggccccaggc aggggtattg gtgggagatg ccgacctcat cgaacgcctc cggtccaacc
     8221 cgctgaccag ggccttgcgg gtcgatagcg tcacctacgc cggcctgggc gccacactgg
     8281 aggtctatct cggtgccgac ccgaccgcca taccgttctg gcgccacgcc ctcacacctg
     8341 tcgactccct cgaaagcagg ctcgagtcat tctcttccgt cacaggcggc gaggtggtgg
     8401 aggcggcttc cgtggtggga gcggggagcg cccccgggct ctcgattccg accccggttc
     8461 tgcgaatcca ggacggcgcc agcatgtttg gagggctgat ctctcagaat cgaccggttc
     8521 tcaccaggag ggacaaggga gatctcatcg tcgacctcag ggcagtcgac ccccaggacg
     8581 acgaggtcgt ggtgactgcg ctcgagagat gccggtaatc gggaccgccg ggcacgtcga
     8641 ccacggcaag tcgactctca tccagcgaat cacagggaga aaccctgatc gttgggcaga
     8701 agaacgcgaa cgcggcctga ccatcgacct cgggttcgcc tggaccacgc tgcccaacgg
     8761 catagaagtc tccttcgtcg acgtcccggg tcacgaacgg tttctgaaga acatgctcgc
     8821 cgggatcgag accatcgact tcgccctctt cgtcgtggcg gctgacgaag gctggatgcc
     8881 ccagtccgag gagcatctcg cagtactcga cctcctcgaa gtgactcatg gcgtgatcgc
     8941 cctgaccaaa tctgatgcgg tcgagcccga tcttctcgat ctggcaacgg tcgagaccga
     9001 agagaggctg gaaggaacgt cccttgccga agcacccatc atggcggtgt ccggcaccac
     9061 aggtgacgga gtcccggacc ttctgaagac catctcggag cacctgcccg agccacccgc
     9121 cagcaccgga aggccccgtt tgtgggtgga ccgtgcattc gcggtatccg gcgccgggac
     9181 cgtggccacc ggaacccttc tcgacggctc gttgcaggtc gacgacggcg tcatggtgta
     9241 cccgagtgga cgagcatccc gggtgcgggg gatccagagc catgagaagt ctctcgacca
     9301 ggtcgaaccg gcccgacgag tcgccctcaa cctcgccggg ctggagcacc aggatctcgc
     9361 acggggtgac atgatcggat ttcccggtca atgggtcgtc tccgaccggt tcgccgtctc
     9421 actcagaaca gcgcggttcg tcgaggagat cgaccagcgc ggcgcctacc agctccacat
     9481 cggatcttcg gcacaccggg tcgtgatcac aggtctggag aacaacgggg ccgtccttca
     9541 actggatgcc gcgatcccgg tcgcggtagg cgaccgattc atcctccgcg ataccggccg
     9601 gagactggct gtcgccgggg ggcgcgtcct cgacccatca ccgatcaaga cgaggatggc
     9661 gatcaaggcc tcctcgcgaa tcgacccgtc ggcgagcccc gatcagatag ccgacacgct
     9721 tctcaccatt cgaggtcggg agcgtgtaca gcggctggcg gctgagagcc ggggcggatc
     9781 g
//
LOCUS       DASUTA010000417         3728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_123341, whole genome shotgun sequence.
ACCESSION   DASUTA010000417 DASUTA010000000
VERSION     DASUTA010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123341"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(309..1535)
                     /locus_tag="VFZ80_08295"
     CDS             complement(309..1535)
                     /locus_tag="VFZ80_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006796515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6287472.1"
                     /translation="MPDEKRRHQPDTESVTLGHEAARHLGSLKPPIYETTTFVFETAE
                     EGKRFFEDAYGPTGARDDDAGYIYSRLDSPNLAPAEARLAAWEEAADAAVFSSGMGAI
                     TTVLLTFLRPGDLVLYSMPTYGGTSTLMKGLLTEFGVVTRPFASTTASEELDALVGQD
                     QLTMVYVETPANPTNELFDIGLAAALADRHEARVVVDNTFLSPVWQKPLVHGADLSLH
                     SATKYLGGHSDLTAGAVSGGTDDVERLRRTRYEIGTTPSPSTGWLLGRSLETLRLRVS
                     QQTSNATRIAAFLNEHDKVARVSHLSLLQPGEPGYEIYKRQCLGPGAMISFEILGGEA
                     ECFRFLDAMEVIRLATSLGGTESLACHPWTMSPPTVPDAEKHSIGVTPGLIRLSVGIE
                     DVADLIGDLDHALSQV"
     gene            1596..2492
                     /locus_tag="VFZ80_08300"
     CDS             1596..2492
                     /locus_tag="VFZ80_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX6287473.1"
                     /translation="MDQFAENLNMDPGTLSNIVRTLAALILVLILRRVARRVVVSSVE
                     DTETSYRANKIINYAATAILLITVAFIWVDAFDNLPTYLGLVSAGIAIALADVLKNMV
                     GWLYILSRRPFQVGDRIEVSGVKGDVVDIRLFRFSLMEVDGWVDAEQSTGRLVHVPNG
                     VVFNKEVANYTEGFGFIWHEVPVLITFESDWKLAERLILEVLERDAPDVHGAAGTHIR
                     ETARRYSIRVGTLTPTVYLTVRDSGVLLTARYLVEARTRRGREDRVWRSILEEFAKHP
                     EVELAYPTVRTYFGGPIRLQSD"
     gene            2503..3270
                     /locus_tag="VFZ80_08305"
     CDS             2503..3270
                     /locus_tag="VFZ80_08305"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEX6287474.1"
                     /translation="MDQLIDEAARVLRPTEQILVFSGAGLSTESGIPDFRGPNGLWTK
                     VDPDDFTIDRYVTNRDLRVQGWRMHVDGELWGARSAVRPNSGHEAIVRLHERRRLAGV
                     VTQNVDGLHYKAGLADDLVAELHGNVRHSHCMSCGARWETETVLKWVEAGEEDPSCPE
                     CSGMIKTDTVMFGEMLPEQEVRQASLFLAMADAVLVVGSTVSVWPAADVVMRAAVQQK
                     PIVIINKGATDADHLAAVKLDAAIGEVLPDLVDRIIG"
     gene            3369..>3728
                     /locus_tag="VFZ80_08310"
     CDS             3369..>3728
                     /locus_tag="VFZ80_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX6287475.1"
                     /translation="MSSYSPPLEEHNFVLIHVAGLEEISKLNGYQHADPATVATIIEE
                     AGRFFAEVMEPLNRTGDQQGSVLDEGGQVHTPEGFGHAYEKLVQSGWPAAHLPADWGG
                     GGLPYVVGVVIQEMFKTS"
BASE COUNT          677 a         1150 c         1218 g          683 t
ORIGIN      
        1 gattcgagcg gccagcggcg agatgacgtc catggcctcg atggtacgag gcttgtatga
       61 gggggtgact ccgaggagtc accccctcag ctccgggtag ggagcacctg tgacagatgg
      121 agggccgtta gacggcgggc gcgagtggat ccgaaagtga ttcctgacgt gagaacgaag
      181 tccaacgcct gcttcattgc ccacttcctc tcgctcagtc tcccggttcc gccccctgac
      241 cacgtagaga ctatctcgcg cggagagtgg tcaagtatgg gttcagcgac ctatccgaaa
      301 agaggtgatc agacctggga gagggcgtga tccagatcgc cgatcaggtc ggccacgtcc
      361 tcgatgccga cggacagcct gatcaggcct ggtgtgactc cgatggagtg cttctcggcg
      421 tccggcaccg tcggggggct catcgtccat gggtgacagg ccagggactc ggttcccccc
      481 agagaggtcg ccagccgaat cacctccatg gcatcgagga agcgaaaaca ctcggcttcg
      541 ccgccgagga tctcgaagga gatcatcgct ccggggccga gacactgccg tttgtagatc
      601 tcgtatccgg gctcgcccgg ctggaggagg ctgagatggc tcaccctcgc caccttgtcg
      661 tgctcgttga ggaaggcggc gatcctggtg gcgtttgagg tctgctggct caccctgagc
      721 cgaagcgttt ccaggctccg gccgaggagc cagccggtgg atgggctcgg tgtggtcccg
      781 atctcatacc gggtccggcg gagacgctcg acgtcgtcgg tgcctccgga caccgcccct
      841 gccgtgaggt cggagtgacc gccgaggtat ttggtggccg agtgcaacga gaggtcggcg
      901 ccatgaacca acggcttttg ccagaccggt gacaggaagg tgttgtcgac cacgacccga
      961 gcctcgtgcc gatccgccag ggcggctgcg agccctatgt cgaagagctc gttcgtggga
     1021 ttcgccggag tctccacata gaccatggtc agctgatcct ggccaaccaa cgcatccagc
     1081 tcctcgctgg ctgtggtcga tgcaaacggg cgggtgacga caccgaactc ggtcagcaac
     1141 cccttcatca gggtcgacgt tcccccgtat gtgggcatcg aatacaggac gaggtcgccg
     1201 ggccgcaaga acgtgaggag gactgtcgtt atcgctccca tcccgctgga gaagacggct
     1261 gcatccgccg cttcctccca cgcggcgagg cgggcttcgg caggagcgag attcggtgag
     1321 tcgagacggc tgtagatgta gccggcgtca tcgtcgcgtg ctccggttgg cccgtaggcg
     1381 tcctcgaaga atcgcttccc ctcttcggcg gtctcgaaca cgaaagtcgt cgtctcgtag
     1441 atgggcggct tgagcgaacc gaggtggcgg gccgcctcgt ggccgagggt gaccgactcg
     1501 gtatcgggtt ggtgtcggcg cttctcgtcg ggcacgggcg gagactaacg gtcgaattcc
     1561 ccgtccagga cagggctcca cccgataatc cctgtgtgga tcagttcgcc gagaatctca
     1621 acatggatcc ggggacgctg agcaacatcg tccggacgct ggccgcactc atcctcgtcc
     1681 tgatcctgcg ccgggtcgcc cgccgggtgg ttgtcagcag tgtcgaggac accgagacca
     1741 gctatcgcgc caacaagatc atcaactatg cggccaccgc gattctcctg atcaccgtgg
     1801 cgttcatttg ggtggacgcc ttcgacaact tgcccaccta tctcggtctg gtctccgccg
     1861 gtatcgccat cgccctcgcc gacgtgctga agaacatggt tggctggctg tacatcctca
     1921 gccggcggcc attccaggtg ggcgatcgca tcgaggtcag cggagtcaag ggggacgtgg
     1981 tcgacatccg gctcttccgc ttctctttga tggaggtcga cggttgggtc gacgccgagc
     2041 aatcgacagg gcggctcgtc cacgttccca acggtgttgt cttcaacaag gaggtggcca
     2101 actacaccga ggggtttggg ttcatctggc acgaggtccc cgtgctcatc accttcgagt
     2161 cggattggaa gctcgccgag cggctgatac tcgaggtatt ggagcgagat gctccagatg
     2221 tccacggcgc cgccgggacc cacatcaggg agacggcgag gcgctattcg atccgggtgg
     2281 ggactctcac ccctaccgtc tatctcaccg tgcgcgacag cggggtcctt ctgacggcac
     2341 gctatctggt cgaggcgagg acgcgccgag gtcgcgagga ccgggtctgg cgctcgatat
     2401 tggaggagtt cgccaagcat cccgaagtcg agctcgccta tccgacggtg cgcacctact
     2461 tcggcggtcc gattcggctc caatccgact agacaggagt cgatggacca actcatcgac
     2521 gaagcggcac gggtcctcag accgacagag cagattctcg tgttcagtgg cgccgggctg
     2581 agcaccgaat cgggaatccc cgactttcgc ggacccaacg ggctttggac caaagtcgat
     2641 cccgacgact tcaccatcga tcgctacgtg accaatcgcg atctgagagt ccagggctgg
     2701 cgcatgcacg tcgacgggga gctctggggg gcgcgctcag cggtcaggcc caattcgggg
     2761 cacgaggcca tcgtccgact ccatgagcgc cgaaggctcg ctggagtcgt cacccagaac
     2821 gtcgacggtc tccactacaa ggccgggctc gctgacgacc tcgtcgccga actgcatggc
     2881 aacgtcaggc acagtcactg catgtcgtgt ggcgcccggt gggagaccga aaccgtgctg
     2941 aagtgggtgg aagcgggtga ggaggatccg tcgtgccccg agtgcagcgg catgatcaag
     3001 acggacacgg tcatgttcgg cgagatgctg cccgagcaag aggtgcgcca ggcgtcgctc
     3061 ttcttggcca tggccgacgc cgtcctcgtc gtcgggtcga cggtgtcggt gtggccggcg
     3121 gcagacgtgg tgatgcgtgc cgccgtccag caaaagccca tcgtcatcat aaacaagggc
     3181 gccacggacg ccgaccacct cgccgcagtc aaactcgacg ccgccatagg ggaggtcttg
     3241 cccgacctgg tcgaccggat aatcggttga tcgaatcgca acaccgtctg cgcggatctg
     3301 tgcacggcag ggtgcgttga ccaacccaca caaacggttg gatcgccccc tcaactacga
     3361 ttttgagtat gtcctcatat tcgccgccgc tcgaggagca caacttcgtg ctcatccatg
     3421 tggccggtct cgaggagatc tcgaagctca atggctatca gcacgccgac cccgccacgg
     3481 tggcgacgat catcgaagaa gcgggccgat tcttcgcaga ggtcatggag ccgctcaacc
     3541 gcaccggtga ccagcagggc tccgttctcg acgagggggg ccaggtccac acaccggagg
     3601 ggtttggcca cgcatatgag aaactcgttc aatcaggctg gcccgcggcc catctcccgg
     3661 ccgattgggg gggcggaggc ctgccctacg tcgttggggt cgtcatccag gagatgttca
     3721 agacgtcg
//
LOCUS       DASUTA010000418         2813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1937970, whole genome shotgun sequence.
ACCESSION   DASUTA010000418 DASUTA010000000
VERSION     DASUTA010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937970"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /gene="rsmA"
                     /locus_tag="VFZ80_08315"
     CDS             complement(<1..585)
                     /gene="rsmA"
                     /locus_tag="VFZ80_08315"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX6287476.1"
                     /translation="MAQTRTQIAELLQRHGVAPIQRLGQHFLADANITRKIVDVAGVT
                     ADSKVVEIGAGTGTLTRALAGTGARVVAYEIDKGLRPVLAEVTQDLGVELRFQDVTDV
                     DLGAALDGEGWTLVANLPYNVGTPVLLDALQHAPNIVEFTVMVQREVAERLTAAVSDD
                     LYGLPSVIAGIHADARIAFIVPPQVFYPPPRVESA"
     gene            complement(585..1331)
                     /locus_tag="VFZ80_08320"
     CDS             complement(585..1331)
                     /locus_tag="VFZ80_08320"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX6287477.1"
                     /translation="MPWVDTHCHLQLDGRPPALLLHRASNVSWLVAPGVDAASSLASL
                     ALAEEFGDRVVATAGLHPHEASSWPEQRAEIESLAPRVGAIGETGLDFYRDLSPRHAQ
                     EAALRDHIEIAHDLDKPLIVHCRDAFSDVHRILSDTGAGPRTVLHCWTGGRRWARRFL
                     ELDVTFSFAGPVAFETGDTIRRSAEVIPPERALVETDTPYLAPPPHRGEPNEPAWVAL
                     VGEALARVWDLAAEDVASITSANASRVFGR"
     gene            1385..1621
                     /locus_tag="VFZ80_08325"
     CDS             1385..1621
                     /locus_tag="VFZ80_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008418553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX6287478.1"
                     /translation="MDSGIARKIDDLGRIVIPAETRRMFNIREGDHLFIAVEGGNIVL
                     RKMTDSCTFCGSDSQVSQFKGKGICSNCRSQLDS"
     gene            complement(1606..2439)
                     /gene="rsmI"
                     /locus_tag="VFZ80_08330"
     CDS             complement(1606..2439)
                     /gene="rsmI"
                     /locus_tag="VFZ80_08330"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX6287479.1"
                     /translation="MSEGRLVVCATPIGNLGDISERLRNTLASADVVYAEDTRRTAKL
                     LRHLVISVPLVSLFVGNEASRTDELVEAVRRGEKVVLVSDAGMPTISDPGAAAIRAVR
                     DAGFPVSVVPGPSAVTMALALSGFGADRFSFEGFLPRKGRARAERIDAIASDERAVVI
                     FASPNRLAEDLRDLETACGSDRNVAVMRELTKLHEECWVGQLGTAVQWWTEEVRGEVT
                     LVVGPAELPGPSIEEAIATARELVAQGGSIRVSARTVAESMGVSRREVYEALISDQES
                     S"
     gene            complement(2459..>2813)
                     /locus_tag="VFZ80_08335"
     CDS             complement(2459..>2813)
                     /locus_tag="VFZ80_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287480.1"
                     /translation="TVSGYELETTSVWGEEGFRVLLNQGTGVSTMAAAADGWGGDSYH
                     QWFDGEKTARLIVYQGDTANDVSELEESLLTFANDSFPEDHFAWVEQLNGNLYFIAAD
                     ETAVGEQIRASVGLD"
BASE COUNT          533 a          919 c          881 g          480 t
ORIGIN      
        1 ggccgactcg acccgcggtg gcggatagaa cacttgggga ggcacaatga acgcaatccg
       61 ggcatcggcg tggatcccgg caatgaccga cggcaacccg tagagatcgt ctgagaccgc
      121 ggccgtgagc ctctcagcca cttctcgctg gaccatcacc gtgaattcga cgatgttcgg
      181 ggcgtgctgc agggcatcga gcaacaccgg ggttcccacg ttgtagggga ggttggccac
      241 caacgtccat ccctcaccgt cgagggctgc gccgaggtcg acatcggtca cgtcctggaa
      301 ccgcagctcg acaccgagat cttgggtcac ttccgccaat accgggcgaa gccccttgtc
      361 gatctcatag gcgacgaccc gggcaccggt cccggccagg gcgcgggtga gtgttccggt
      421 gccggctcct atctcgacca ctttcgagtc tgcagtcacg cctgcgacgt cgacgatctt
      481 ccgcgtgatg ttggcgtcgg cgaggaaatg ttgacccaga cgctgtatcg gggctacacc
      541 atgacgctgc aacaactcgg cgatctgggt gcgagtctgc gccatcagcg accgaagacc
      601 ctgcttgcgt ttgctgatgt gatcgaggcg acgtcctccg ccgccaggtc ccagactctc
      661 gccagggctt ccccaaccag agcaacccaa gccggctcat tgggctcccc gcgatgaggc
      721 ggcggagcca aatagggcgt gtcggtctcg actagcgcac gttcgggcgg tatcacctca
      781 gcactgcgtc ggatcgtgtc tcccgtttcg aacgcaacgg ggccggcgaa ggagaaggtg
      841 acgtccaatt cgagaaagcg cctggcccac cggcgcccgc ccgtccaaca gtgcaggact
      901 gtccgaggtc cggccccggt gtcgctcagg atccgatgga catcggagaa cgcatcgcgg
      961 cagtggacga tgagaggttt gtcgaggtcg tgggcaatct cgatgtggtc acgtagagcg
     1021 gcttcttggg cgtgacgggg cgacagatcc cggtagaagt cgagcccggt ctctccgatg
     1081 gctccgacgc gcggcgcaag ggattcgatc tcggcgcgct gctcgggcca cgacgacgct
     1141 tcgtgcggat gaagtccggc cgtggccaca acccgatcac cgaactcctc ggccaatgcc
     1201 agagaggcca gggagctggc cgcgtcgacg ccgggagcca ccagccacga gacattcgac
     1261 gctcggtgca acagcagcgc cgggggccgt ccatcgagtt gaaggtgaca gtgggtgtct
     1321 acccagggca tcgacgagga cggtacctgg caccacgcca atcccgatcg gtaggctcgg
     1381 cgaggtggac tctggaatag ctcgcaagat cgacgacctg gggcgaatcg tcatccccgc
     1441 cgagacgaga cggatgttca acatccggga gggcgatcac ctctttatcg ctgtcgaggg
     1501 cggcaacatc gtcctccgca agatgacaga ctcgtgcact ttctgcggaa gcgatagcca
     1561 ggtcagccaa ttcaagggaa aagggatctg tagcaactgc cgatctcagc tcgactcctg
     1621 atcgctgatc agggcctcgt agacctcgcg acgggagaca cccatcgact cggcgacggt
     1681 acgggcagac acccggatgc tcccgccttg cgccaccaac tcgcgggccg ttgcgatggc
     1741 ctcctcgatc gatggcccgg gcagctcggc cgggccgacg acgagggtca cctcgcctct
     1801 gacctcttcg gtccaccatt gcaccgccgt tcccagctgg ccgacccagc actcctcgtg
     1861 gagtttggtc agctccctca tgacggccac gttgcggtca ctgccgcagg cagtctccag
     1921 gtcacgtagg tcttcggcga gccggttggg gctggcgaat atcacgacag cccgttcgtc
     1981 ggaggcgatg gcatcgattc gctcggcgcg ggcccgtccc ttccggggca gaaacccctc
     2041 gaacgagaat cgatccgcgc caaagccgga gagtgccaac gccatcgtga ctgccgacgg
     2101 ccccggaacc accgacacgg ggaagccggc gtcccgaacc gctctgatgg cagcggcacc
     2161 ggggtcggag atcgtcggca tgccggcgtc tgacaccaaa acgaccttct cacctctcct
     2221 caccgcttcg accaactcgt cggtgcgact cgcctcgttg ccgacgaaga gagacacgag
     2281 gggcacgctg atcacgaggt gacgcagcag ttttgcggta cgacgggtgt cctcggcgta
     2341 gacgacgtcg gcagaggcca acgtgttgcg gagacgttcg gagatgtcac ccagattgcc
     2401 gatcggagtg gcacagacga ctagtcgacc ttcggacaca ttccgaagtt agaggatgtc
     2461 agtccaaacc aacagaggct ctgatctgct ccccgaccgc ggtctcgtcc gcggcgatga
     2521 agtagaggtt cccgttcagc tgctccaccc atgcgaaatg atcctccggg aacgaatcgt
     2581 tggcaaaggt gagaagggac tcttcgagct cggaaacatc gttggcggtg tctccctgat
     2641 agacaatcag ccgcgctgtc ttttcgccgt cgaaccattg gtgatagctg tccccacccc
     2701 agccgtctgc cgccgccgcc atggtcgaca caccggtgcc ctggttgagt agaacccgga
     2761 aaccctcctc gccccacacc gacgtcgtct ccagctcgta ccccgagacc gtc
//
LOCUS       DASUTA010000419         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_254697, whole genome shotgun sequence.
ACCESSION   DASUTA010000419 DASUTA010000000
VERSION     DASUTA010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254697"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VFZ80_08340"
     CDS             complement(<1..758)
                     /locus_tag="VFZ80_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX6287481.1"
                     /translation="MKSTLRYAWFVLGFNVLVILMGALVRATGSGAGCGSSWPTCEGR
                     IIPELDGATAIEFTHRAASGVALALVAILAALVWRRTERGHQARLGSTLSLVAIVGEA
                     LIGALIVAAEWVADDASLARAISVPLHLVNTLFLLAALALTVFWLDGGGRLALRRDRT
                     TTLAVVLGGLALILLFASGAITALADTLFPKTPGSDLTSEAHFLTDLRIVHPVLAVVA
                     AGIGWWVSVRSRGVLAGWARWLPALVGLMLVTGA"
     gene            811..936
                     /locus_tag="VFZ80_08345"
     CDS             811..936
                     /locus_tag="VFZ80_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287482.1"
                     /translation="MDPSPILAQQGEYWLAGILTVVAAAIVTVVILVSSRSSDRS"
     gene            942..1886
                     /locus_tag="VFZ80_08350"
                     /pseudo
     CDS             942..1886
                     /locus_tag="VFZ80_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013415279.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
BASE COUNT          448 a          693 c          694 g          356 t
ORIGIN      
        1 atcgctcccg tcaccagcat taggcccacg agggccggga gccatctcgc ccatccggcg
       61 aggacgcctc tgctgcggac agagacccac cagccgatgc cggccgccac cacggcgagc
      121 acggggtgga cgattcggag atccgtcagg aagtgtgcct cggaggtcag gtcagagcca
      181 ggggtcttgg ggaagagagt gtccgccagt gccgtgatcg cacccgaggc gaacagcaag
      241 atcaacgcca acccgcccaa caccaccgcc agggtggtgg tgcggtcacg acggagagcc
      301 agcctgcccc caccgtccaa ccagaagaca gtcagtgcca gggcggcgag aaggaaaagt
      361 gtgttgacta ggtgaagggg caccgatatc gcccgggcca gcgacgcgtc gtctgcaacc
      421 cactccgcgg ccacgatcaa ggcaccgatg agagcttctc cgacgatcgc caccaaagag
      481 agggtcgacc cgaggcgagc ctggtggccc cgctcggtgc gacgccatac gagggcagcc
      541 aggatcgcca ccagagccag agcaacgccg cttgcggcgc ggtgagtgaa ctcgatcgct
      601 gtcgccccat cgagttcggg gatgatccga ccctcgcagg tgggccatga gctcccacat
      661 ccggcgccac tcccggtagc gcgcaccagt gctcccatca gtatcaccag aacgttgaat
      721 ccgaggacga accatgcgta tctgagggtc gacttcactg ggaggaggtt aggtatcggc
      781 tgggttagac ggatatgcca tcatcaccgc gtggacccct caccgatcct cgcccaacag
      841 ggcgaatact ggctggccgg gatactgacc gtcgtggcag cggccattgt caccgttgtc
      901 atcctggttt cctcgcgctc gagcgatcgt tcctgagcct catggagatc cacgagagca
      961 tcatcgacac catcgggaac acgcccctcg tgcgtctatc ccgccttcat ccgccgggaa
     1021 acctggtggc caaactggag tacacgaatc cgggaggatc cgtcaaagaa cggatcgctc
     1081 tcgccatgat cgaagcagcg gaggcatcag gcgcgctccg tccgggcggg accatcatcg
     1141 agcccacctc cggcaacaca ggcgttggcc tcgccctggt cgccgcagtg cggggatacc
     1201 gggtgatctg cacggtcccg gacaaggtat ccgaggagaa acgagacctc ctgcgagcct
     1261 acggcgtgca ggtcgtggtc gctcccacgg aatacggtcc cgaccatccc gactcctact
     1321 acggggtggc caacaagctc gcctccgaga tcgagaactc attccaaccg aatcaatact
     1381 tcaacgaggc caacccgcgg tcccactacg agaccacggg ccccgaggtc tgggagcaga
     1441 ccgggggcga gatcgacgtg ttcgtggcag gtgtgggaac aggtggcacc atcacgggcg
     1501 ccggccgctt tctcaaggag aagaagccgt ccatcgagat cgtgggtgcc gaccccgaag
     1561 gttcgatata caccaaggga ccagaaggag acatccatca atacgaggtc gagggtgtgg
     1621 gcgaggactt ctacccggtg acagtcgatc tcgacgtcat cgatcgcttc atcgaggtca
     1681 gcgacgagga atcgtttgcc atggcgcgca ggctcgcccg ggtagaagga gtcctcaccg
     1741 gtggctcggg aggcctcgcc gtcgaggcgt cgctgcgtgt cgcagaggag gacaccggtc
     1801 gtctggtggt agtgatcgtc cccgactcgg gtcgggggta tctgtcgaag ctgttcaacg
     1861 acgagtggat ggagtcgaaa ggcttcagca cgtaagtgac cccgccggtg attccggcgg
     1921 ggtcacgatc ttgcggccct ggcatttctt cacctgtagt gccgcggttg gacgaatccc
     1981 acggtatcca gagccgggtg gtccaagttt ggagtcgcca cctgtcccaa atcgggaacg
     2041 agcgacgctt cagcggtcag cggcgctcag cgcttggcca cctccagagt cctgaaacat
     2101 tcatgcaagt tcggaccacc tcctttctct ggtagcgcga ttgtgccaca gatcggcgag
     2161 ccaacaaacg gatttcggcg gaaccggtat c
//
LOCUS       DASUTA010000420         2628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_124205, whole genome shotgun sequence.
ACCESSION   DASUTA010000420 DASUTA010000000
VERSION     DASUTA010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124205"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VFZ80_08355"
     CDS             <1..649
                     /locus_tag="VFZ80_08355"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6287483.1"
                     /translation="ATICRWLRASSSADVDAEAWDERYRASELLWSAGPNIWVERRLA
                     DAEPGRGLDLASGEGRNAIWLAKRGWEMTAVDFSSVAIERGRSLSDQVEFIEADVLTW
                     EPEATDGSRGFDLVLIAYLQLEAEPLSDVVRRATTWVDPGGELFMVGHDISNLDEGVG
                     GPQVPEILWDLDLMLEWLGDLHIVEAGIAKRPVEVDGDIEYARDTLIRARASESA"
     gene            complement(621..1079)
                     /locus_tag="VFZ80_08360"
     CDS             complement(621..1079)
                     /locus_tag="VFZ80_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015330504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HEX6287484.1"
                     /translation="MGFHLAQCNVGRIIRPLDHDEMADFVAALDPINALAEASPGFVW
                     RLKDDDGQSSSYVDIPGSDDPLLIINYSIWEDLDSLKHFVNKTRHVDYLRRRREWFEK
                     LDVPTSVAWWVDAGTIPDVGEAFRRVLLLQENGPTPEAFTLSRPTPMPVP"
     gene            complement(1081..2232)
                     /locus_tag="VFZ80_08365"
     CDS             complement(1081..2232)
                     /locus_tag="VFZ80_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEX6287485.1"
                     /translation="MGFRTKQVHAGVTPDPETGAILTPIHQSTTFVQESVDDYMGKGY
                     SYTRAGNPTVRALEEKLTALEGGVDSTAYGSGMAATVAVFLALLESGDHIVTSDVVYG
                     GTYRFADQFLRKFGVGVSFVDTSDTVPVEDAITESTKLILTETPANPTLKVTDLAAVS
                     AICGERGLIHVTDNTFLTPYFQRPFELGADVIIHSTTKFLDGHNATLGGAVVVNDAEL
                     QEKIAFARLSAGLVMSPMVAWLTLQGTKTLSARMDAQSASAMAIAEFLDTHEKVDYVN
                     YPGLPSHPQHELSSKQASGYGAMVCFEVHGGVEAGKRLMDNIELWSLAENLGSVESLI
                     THPVTMTHAAVPRQERIASGITDGLVRLSVGLEDPEDLIAALDGALGHV"
     gene            complement(2308..>2628)
                     /locus_tag="VFZ80_08370"
     CDS             complement(2308..>2628)
                     /locus_tag="VFZ80_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX6287486.1"
                     /translation="RRLVKADTGDQLLTFKDLELNPLNYQATLAGEPMDLTYMEYELL
                     RFLVENPVRVWSREQLLSKVWGYEYYGGARTVDVHVRRLRSKLGEERASWITTVRSVG
                     YRWG"
BASE COUNT          451 a          787 c          890 g          500 t
ORIGIN      
        1 ggcgacgatc tgccgttggc tcagggcatc gagttcggct gacgtggacg ccgaggcctg
       61 ggacgagcgc tatcgagcgt ccgagctcct ctggtccgcc gggcccaaca tctgggtgga
      121 gagacggctc gccgacgccg aaccgggccg gggcctcgac ctggcctcgg gtgagggacg
      181 caatgcgatc tggctggcca aacgcggctg ggagatgacg gcagttgatt tctccagtgt
      241 ggcaatcgag cggggccgga gtctgtccga ccaggtcgaa ttcatcgagg ccgacgtcct
      301 cacctgggag ccggaggcga ccgatgggtc taggggcttc gacctggtgc tgatcgccta
      361 tttgcagctg gaggcagagc cgctctcgga tgtcgttcgc cgggcgacca cctgggtcga
      421 tcccggtggt gagctgttca tggtcggcca cgacatctcc aatctcgatg agggcgtcgg
      481 ggggccccag gtgcccgaga tcctttggga tctcgatctg atgttggagt ggctgggaga
      541 tctccacatc gtcgaggctg gtatcgccaa gcgtccggtc gaggtggacg gcgacatcga
      601 gtacgcccgg gacaccttga tcagggcacg ggcatcggag tcggcctaga gagggtgaac
      661 gcctcggggg tcgggccgtt ctcctgaagg agaaggaccc ttctgaaggc ctctcccaca
      721 tccgggatgg tcccggcatc gacccaccag gccacgctcg tcggtacgtc gagcttctcg
      781 aaccactcac gtcgccgacg cagatagtcg acgtggcggg tcttgttcac gaagtgcttg
      841 agactgtcca ggtcctccca gatcgagtag ttgatgatca gcaaggggtc gtcgctgccg
      901 gggatgtcga cataggacga cgactgcccg tcgtcgtcct tgagtctcca cacgaagcca
      961 gggctggcct cggcgagggc gttgatcgga tcgagggcgg cgacgaaatc ggccatctcg
     1021 tcgtgatcga gaggtcggat gatgcgccca acgttgcact gggcgagatg gaagcccatc
     1081 tcagacgtgg cccagcgccc cgtccaatgc ggcgatgagg tcttcggggt cctcgagccc
     1141 gaccgagagg cggaccaggc cgtcggtgat tccggaggca atgcgctcct gacggggcac
     1201 cgcggcgtgc gtcatggtca caggatgggt tatcagagac tccaccgatc ccaggttctc
     1261 ggccaggctc cacagctcga tgttgtccat caggcgtttc ccagcctcca cgccaccgtg
     1321 gacctcgaag cagaccatcg ccccgtatcc gctcgcctgt ttgctggaga gctcgtgttg
     1381 gggatggctg gggagcccgg ggtagttcac gtagtcgacc ttctcgtggg tgtcgagaaa
     1441 ctcggcgatg gccatggcgc tcgccgactg agcgtccatg cgggcgctga gcgtcttggt
     1501 cccctgaaga gtgagccagg caaccattgg tgacatcacc aggccggctg agaggcgcgc
     1561 gaatgcgatc ttctcctgga gctcggcgtc gttcaccacc accgctccgc cgagtgttgc
     1621 attgtggccg tcgagaaact tggtcgtcga gtggatgatc acgtcggcgc cgagctcgaa
     1681 cggtctctgg aagtaagggg tgaggaacgt gttgtcggtg acgtggatca atccccgctc
     1741 gccgcagatg gccgaaacag cggccagatc ggtcactttg agtgtggggt tggccggggt
     1801 ctcggtgagg atgagcttgg tgctctcggt tatggcgtct tcgactggga ccgtgtccga
     1861 cgtgtccacg aagctgaccc cgacaccgaa cttcctcagg aactgatcgg cgaaccgata
     1921 ggtgccccca tatacgacgt cactggtgac gatgtgatcg ccgctctcca agagggcgag
     1981 gaacactgcg accgtcgccg ccatccccga gccatatgcg gtggagtcga caccaccttc
     2041 gagagcggtg agcttctcct cgagggcccg gaccgtggga tttcccgccc gggtgtacga
     2101 gtaacccttg cccatgtagt catcgacaga ctcctgcacg aacgtcgtcg actgatggat
     2161 cggggtgagg atggcccctg tctccggatc gggcgtgacc ccggcgtgta cctgcttggt
     2221 gcgaaaaccc atctcgcgat tgtgccacgc ctcgtgcgtg tgcacgctta tgcccctcta
     2281 acccgaacgc accccgtgca acctctctta tccccagcgg tagccgacgg agcggaccgt
     2341 ggtgatccaa gaggcgcgtt cctcgccgag tttggagcgg agacggcgga cgtggacgtc
     2401 gaccgtgcgc gccccgccgt agtactcata gccccagacc ttggagagga gctgttcgcg
     2461 cgaccagacc cggaccgggt tctcgacgag aaagcggagc agctcgtact ccatgtatgt
     2521 gaggtccatg ggctcgccgg cgagagtcgc ttgatagttc agtgggttga gctcgaggtc
     2581 tttgaaagtc agaagctggt cgccggtgtc ggccttgacc agacgtcg
//
LOCUS       DASUTA010000421         4233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1939742, whole genome shotgun sequence.
ACCESSION   DASUTA010000421 DASUTA010000000
VERSION     DASUTA010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1939742"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="VFZ80_08375"
     CDS             complement(<1..1004)
                     /locus_tag="VFZ80_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6287487.1"
                     /translation="MSLEARIRLRRSEELDIDLDLSIPPGATVALLGPNGAGKSTVVA
                     ALAGLIPIDSGRISLDERVLDDPDSDVFVPAEQRNVGVVFQDYLLFPHLTVLENVAFG
                     LLSRGVARDVAMHRSTAWLSDVGLEGMEQRRPPELSGGEAQRVALARALVGEPDLLLL
                     DEALSALDVVARASTRHLLQRHLEAFPGPRLLITHDPTEAFLLASEIHVMEGGEVTQS
                     GSADDIRLRPLTPYAADLAGANFFRGSAKEGNVDVGGHRLSVADDAVAGPVLVTILPS
                     AVSVHSTRPTGSPRNAWSTTVSVIEPLGPRVRLRTGGPLPMTVELTAAATEEMELLPG
                     "
     gene            complement(1001..1813)
                     /locus_tag="VFZ80_08380"
     CDS             complement(1001..1813)
                     /locus_tag="VFZ80_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006134306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6287488.1"
                     /translation="MSTSERPKSRRVQRPPTLLLILAGTGLALFAVPLVGLVARVPWG
                     SLPELLASEIVIDAFRLSLVASLSATAIAFVLGIPLAWVLARLEFPGRALVRGIVTLP
                     LVLPPVVGGAALLFALGRRGLVGEVVYEATGLLLPFSAWGVVVATTFVAMPFMVITVE
                     GALRSLDQRYEGAATTLGAGRWTVLRRVTLPMIAPSILSGVVLTWARAFGEFGATITF
                     AGNLQGETQTLPLAVFVTLESDRDAAVAISLLMVVVSLAVLIALRDRWWRSV"
     gene            complement(1810..2556)
                     /gene="modA"
                     /locus_tag="VFZ80_08385"
     CDS             complement(1810..2556)
                     /gene="modA"
                     /locus_tag="VFZ80_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6287489.1"
                     /translation="MRRPTTAITFLVLLISLAGCARDSSDQVLVSAAASLTNAFSEIA
                     TAYEEAHAGVDVVLNFAGSSTLREQILEGAPVDVFASANQSNMEVIVDGGLQANDPVI
                     FAENLLQIAVPPGNPAAVSGLEDFAEEGPLFGLCAPEVPCGDFARQALANGGVDPAID
                     TNEPDVRALLTKIEAGELDAGIVYVTDLVSSGGAVEGVAIPDDLNVIAEYPIVVIEDA
                     PNPDGAVGFVEFVLSDEGRSILIEHGFAAP"
     gene            2601..3125
                     /locus_tag="VFZ80_08390"
     CDS             2601..3125
                     /locus_tag="VFZ80_08390"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6287490.1"
                     /translation="MPADPVLSVTEHAVLGILCGGPSHGFAISKQLDADSEVGKVFTV
                     PRTLVYRALGRLVDSGYARQVTTEQRGGPRRVVHEATAAGDRELRHWLAVPVDHIRDL
                     RIEFLLKLTLLRRAGDSPLHLIRSQQERLAPTFDALGDTAIDPRDHVEQWRRSNAQAA
                     MSYLDTLEDAFLEM"
     gene            3178..3834
                     /locus_tag="VFZ80_08395"
     CDS             3178..3834
                     /locus_tag="VFZ80_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019050306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEX6287491.1"
                     /translation="MSLLDRATRHAALGDPRRLLIVDHLLTGDYTVAELAQLVDMPGN
                     LLAHHLDVLDDATLITRGTSEGDHRVRYVTLNRTALPSLRLTEPSLDGLVAFVCTHNS
                     ARSQFAAALWREATGGQSTSAGTHPADRVHPKAARVAMEFGVDISEEVPTGYDDLPGE
                     LDVVVSVCDRAHEAGVPRAGNTLHWSVPDPVPMGTLAAFRSSFEDIARRIEHLSAKST
                     "
     gene            3831..>4233
                     /locus_tag="VFZ80_08400"
     CDS             3831..>4233
                     /locus_tag="VFZ80_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX6287492.1"
                     /translation="MRRKMVAEALGTALLLYIVVGSGIAAQQLVGDRGVGLLAHALAV
                     GLGLGVLIAMLQTVSGAHFNPSVTLAFWRLRAIDRSQAAGFAVAQIAGGIIGVALANW
                     SFGEPPIAFSSSERAGAGLLVAEAIATFVLVL"
BASE COUNT          818 a         1343 c         1366 g          706 t
ORIGIN      
        1 ttccccggga gaagctccat ctcctcggtg gcggccgccg tgagctcgac cgtcatcggc
       61 agggggccac cggtgcgcag tcggactcga gggccgagcg gctcgatgac ggaaacggtc
      121 gtcgaccacg cgttgcgagg gctgcccgtg ggccttgtcg agtgaaccga gacggcactg
      181 gggaggatcg tgaccagtac gggacccgct accgcatcat cagccacgct caaacggtgc
      241 cctccgacat cgacattccc ttctttggcg gaaccacgga aaaagttggc accggccaga
      301 tccgccgcat atggagtgag cgggcgcagc ctgatgtcgt ctgcgctgcc cgattgggtg
      361 acctcacccc cttccatcac atggatctcg ctggcgagga ggaaggcctc tgttgggtcg
      421 tgtgtgatga ggaggcgggg gccggggaag gcctccaagt gtcgttgaag gagatgccgc
      481 gtcgacgccc tcgccacgac atccaacgct gagagtgcct cgtcgaggag aaggagatcg
      541 ggctccccta ccagagcgcg agccaacgcg actctctgcg cctcgccgcc tgacagttca
      601 gggggtcggc gctgctccat cccctccaga cctacgtcgg agagccacgc cgtggacctg
      661 tgcatcgcaa cgtcgcgggc cacaccccgg ctgagcaggc caaaagccac gttttcgaga
      721 acggtgagat gcgggaaaag gaggtaatcc tggaatacga caccgacatt ccgctgctcg
      781 gctggcacga agacatccga gtccggatcg tcgagcactc tctcatcgag ggaaatccga
      841 ccggagtcga tggggatcaa cccggcgagg gcggcgacga cggtcgactt gccggcgcca
      901 ttgggcccaa ggagggcgac cgtggcaccg ggcgggatcg agagatcgag gtcgatgtcc
      961 agctcctccg atcgccggag ccggattcgg gcctcgagac tcacaccgac ctccaccatc
     1021 ggtcccgcaa ggcgatcagg accgcaagtg aaaccaccac catcaagagg cttatggcga
     1081 cagcggcgtc tcgatcgctc tccagagtga cgaagacggc gagcggcaag gtctgagtct
     1141 cgccttggag gttgccggcg aaggtgatcg ttgccccgaa ctcgccaaag gctctcgccc
     1201 aggtgagcac caccccggag agaatcgatg gggcgatcat cggcaaggtg accctgcgca
     1261 aaacggtcca acgcccggcg ccgagagtgg ttgcagcccc ttcgtagcgc tggtcgaggc
     1321 tgcgcagcgc cccttcgacg gtgatgacca tgaaaggcat ggccacgaag gtcgtcgcca
     1381 cgaccacacc ccaggcagag aagggaagga ggagacccgt ggcctcgtag acaacctcgc
     1441 caacgagacc ccgtcgcccg agggcgaaga gcagcgccgc tccaccgacg accggtggga
     1501 ggaccagcgg aagggtcact atgcccctca ccagggcccg acccgggaac tccaaccggg
     1561 ccagaaccca tgccagcggg attcccaaga cgaacgcgat cgcggtggcc gagagtgagg
     1621 cgaccagcga cagccggaaa gcgtcgatga cgatctcact ggcgaggagc tcagggaggc
     1681 tcccccaggg aacacgggcg acgaggccga cgagaggcac cgcgaagaga gccagcccgg
     1741 taccggccag gatcagaagc agagtcgggg gacgctggac acgtcggctc ttgggtctct
     1801 ccgacgtgct catggtgcgg cgaaaccatg ttcgatcaag atggatcgac cctcgtccga
     1861 gagcacgaac tccacgaagc cgaccgcgcc atcggggttg ggagcatcct cgatgacgac
     1921 gatcggatac tccgcgatca cgttcaagtc atcggggata gcgacgcctt cgactgcgcc
     1981 cccgctcgag acgaggtccg tgacgtagac gatccctgcg tcgagctcac cggcctcgat
     2041 cttggtgagc agtgcgcgca cgtccggctc gttggtgtcg atcgcgggat cgacacctcc
     2101 attggccaac gcttgtcggg cgaagtcacc acacgggact tcgggggcac acagaccgaa
     2161 aagcgggcct tcttcagcga agtcttcgag tcccgacacc gcggcggggt ttcccggagg
     2221 caccgctatc tgcaacaggt tctcggcaaa gatgacagga tcgttcgcct gtaggccacc
     2281 gtcgacgatg acctccatgt tcgactggtt ggccgaggca aacacatcca ccggagcccc
     2341 ctccagtatt tgctctcgaa gggtggagct ccctgcgaag ttcaacacga catcgacgcc
     2401 ggcgtgcgcc tcctcgtacg cggtagcgat ctcggagaat gcgttggtga gggaggcggc
     2461 tgcggaaacc aggacctggt cgctcgaatc ccgggcgcac ccggcgagtg agatcaacag
     2521 cacaaggaag gtgatcgcgg tggtgggtct tctcatccct agtattcaca atgtgactag
     2581 atggtcccgt cacactagcc atgcccgctg acccggttct gtccgtgacc gagcatgcag
     2641 tgcttggaat cctctgcgga ggccctagcc atggcttcgc catctcgaag caactggatg
     2701 cagatagcga ggtgggcaag gtgttcaccg tccccaggac cctcgtctac cgggctctcg
     2761 gccgtctcgt ggatagcggc tacgcccgac aggtgacaac ggagcagaga ggtggtccca
     2821 ggcgtgtggt gcacgaagcc actgccgcgg gagaccggga gcttcgtcat tggctcgccg
     2881 tcccggtgga ccacatccgg gacctccgca tcgagttcct cttgaagctc acactgctga
     2941 ggcgagccgg agactcacct cttcacctga tcaggtccca gcaagagaga ctggctccca
     3001 ccttcgacgc gctgggcgac accgcgatcg acccccgcga ccacgtggag cagtggagga
     3061 gaagcaatgc ccaggctgcc atgagctacc tggacacgct cgaggacgcc tttctcgaga
     3121 tgtgagcaat ccggtatcat ttgctcgatt tctattgact caatatagtt tgagttgatg
     3181 tcactgttgg accgcgccac cagacatgct gctcttgggg accccagacg cctgttgatc
     3241 gtggatcatc ttctgaccgg cgactacacg gttgcggagc tggcccagct cgtcgacatg
     3301 ccaggcaacc tgttggccca ccatctagac gtcctcgacg atgcgacgct gatcacacga
     3361 gggacttcag aaggcgacca cagagtcagg tacgtgaccc tcaaccgcac cgcactgccc
     3421 tctttgaggc ttactgagcc aagcctcgac gggctggtcg ctttcgtgtg cactcacaac
     3481 tcggccaggt cgcaattcgc cgcagcgctc tggcgggagg cgaccggtgg gcaatcgaca
     3541 tccgcgggaa cccacccggc cgatcgggtt cacccgaagg cggcccgagt ggcgatggag
     3601 ttcggggtcg acatctccga ggaggtgccg actggctacg acgacttacc aggtgagctc
     3661 gacgtggtcg tgagtgtgtg tgaccgggcg cacgaggccg gtgttcctcg agccggcaac
     3721 acgcttcatt ggtcggttcc agaccctgtg ccgatgggga cgctcgcggc cttccggtcc
     3781 tcgttcgagg acatcgcccg acgcatcgaa cacctgagcg cgaagtcgac gtgagacgca
     3841 agatggtggc ggaagccctc ggcaccgcgt tgctcctata catcgtggtg ggaagtggca
     3901 tcgccgccca acagctggtc ggggaccgtg gtgtcggatt gttggcgcac gcactggccg
     3961 tggggctcgg gctcggagtg ctcatcgcca tgcttcaaac ggtctcgggt gcgcacttca
     4021 atccttcggt caccctcgct ttctggcgtc tgagggccat cgaccgctca caagccgcag
     4081 gtttcgccgt ggcacagatc gcagggggaa tcatcggggt cgctctggcc aactggagct
     4141 tcggagagcc gcccatcgct ttctcctcgt cggagagggc tggagcaggg ctactcgtgg
     4201 ccgaggccat cgccaccttc gtcctcgtac tgg
//
LOCUS       DASUTA010000422         4529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_386265, whole genome shotgun sequence.
ACCESSION   DASUTA010000422 DASUTA010000000
VERSION     DASUTA010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386265"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VFZ80_08405"
     CDS             <1..737
                     /locus_tag="VFZ80_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6287493.1"
                     /translation="GLMPDMEYEERTHRLEDGDVMVLTSDGITEAHSPDGEMYGFSRL
                     MGRVARKTNEQDMCNALVADLEKWTGGEAEQEDDITLVVVRKGASASESASVFDLDLA
                     DELLTTFTVPSAEGNERVAIEKVAEAVGPLGLSTSRVEKLKTAVGETVMNAIEHGNEN
                     NPNLEVTVAVSATSDVVRVTVTDMGGDKEIPEAVVPDLEAKLAGEQTPRGWGLFLIGQ
                     MVDEVNTARENGHHIVELVMSREGEI"
     gene            737..1072
                     /locus_tag="VFZ80_08410"
     CDS             737..1072
                     /locus_tag="VFZ80_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6287494.1"
                     /translation="MPGGEFQAKVKPGADSSMIELVGLVDRGAKEEMERAYQEAVQAP
                     GEILLDFKDVTYINSTGIAVIVGVLAMARAENRKVGARGLTDHYKEVFEITRLADFMH
                     IYEDATAGN"
     gene            1082..1426
                     /locus_tag="VFZ80_08415"
     CDS             1082..1426
                     /locus_tag="VFZ80_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6287495.1"
                     /translation="MPTANLTMNIREVSEGIRVIDVVGEITAFSEKEISEAHEKAGDG
                     ATAVILNFEGLDYMNSGGIGLLVTTLIRAQRAGHALRAYGLTEHYREIFNLTRLDEAI
                     GIYDDEAAAIAG"
     gene            complement(1526..2914)
                     /locus_tag="VFZ80_08420"
     CDS             complement(1526..2914)
                     /locus_tag="VFZ80_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6287496.1"
                     /translation="MSDFDATVVGAGPNGLAAAVELARSGRKTLLIEAADDIGGGTRT
                     EELTLPGFLHDVCSAIHPAGIASPYFNEIGLEVDWVNPPIPVTHPLDGGRVAVLRRSV
                     DETASGLGVDEESYRKLMAPIVEQIEDYVRVALGPVKVIPDHPGAFTRLGLLGGLPAA
                     ALAKRFDTEEGKGLFSGIAAHAIAPFYGLATSAVGLMLGAIAHSHGWPLARGGSRSIT
                     DALAARFEELGGKIELGRTVDSVDDLPPGPVFLDVMPPAAYQIAHTRISPGKVRQLSR
                     WQPGPGIFKVDWALDGPIPWADPSSGQAATVHLGGRYAEVAASERAVARGDHPKRPFV
                     LLAQQSLFDDSRAPDGKHTAWAYCHVPNGSNVDMTVAIEDQVERFAPGFKDIVLQRAT
                     RGPIGYQSYNPNYIGGDISGGQYGLRKLLQIGAESPYSLGGEVYLCSSATPPGAGVHG
                     MCGYHAVRAALD"
     gene            3019..3699
                     /locus_tag="VFZ80_08425"
     CDS             3019..3699
                     /locus_tag="VFZ80_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287497.1"
                     /translation="MTDPRNADNWAKPIDVFHVDETIQGVNKGNVEGRRPTGPLHGFG
                     QLWQKSYEISIPGPPPEEVVQAWKDNFAELWYPGNKFYAPAGGIAPGEVAVIAGGRGP
                     TKVTSGVRVIYADERSWSYMTPEGHPWAAIITFSTHDEEDGTKARIDLMVRANDPLYE
                     VSFHIYTSRLEDKIWTHMLTALAASFGSKGGVVQRTALIDKRRQWREWRNIWKNSAVR
                     TLLRRDRR"
     gene            complement(3769..4305)
                     /locus_tag="VFZ80_08430"
     CDS             complement(3769..4305)
                     /locus_tag="VFZ80_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287498.1"
                     /translation="MTAITPDVRLPEADVPYKKSFILLATAILALAVGFGSMAGGIFG
                     AWYTWDHAAAQNVVTPDDAAIPETAVRGPFTMWAQADIITHHQLENTDGLYYAEMDRM
                     VPQVDENGEVVLDENGEALMVPNEARASWLNATTLTTALSLGIVSYALAAFAFFTGLT
                     LVAVGFVFLQIRKKAVLL"
BASE COUNT          945 a         1308 c         1485 g          791 t
ORIGIN      
        1 tggggctgat gccggatatg gagtacgagg aacgcaccca tcgtctcgag gacggggacg
       61 tgatggtcct caccagcgat gggatcaccg aagcccacag tcccgacggc gagatgtacg
      121 ggttctcccg cctcatgggg cgcgtggcgc ggaagaccaa cgagcaggac atgtgcaacg
      181 ccttggtcgc tgacttggag aagtggaccg ggggcgaggc tgagcaggaa gacgacatca
      241 ctctcgtggt ggtccgcaag ggcgcatcgg ccagcgagtc cgccagcgtc ttcgacctgg
      301 accttgccga cgagcttctg accacattca cagtccccag tgcggaaggc aacgagcgtg
      361 tcgcaatcga gaaggtggct gaggccgtcg gtccgctggg cctcagcacc tcgagggtgg
      421 agaaactgaa gacggctgtc ggtgagacgg tgatgaacgc catcgagcac ggaaacgaga
      481 acaacccgaa tctcgaggtg acggtcgcag tgtcggcgac atcggacgtg gtccgagtca
      541 cggtcaccga catgggcggt gacaaggaga tacccgaagc agtcgtcccc gatctggaag
      601 ccaagctcgc cggcgagcaa accccgcgcg gttggggatt gttcttgatc ggtcaaatgg
      661 tggacgaggt caacacagcc cgtgagaacg ggcaccacat cgtcgaactg gtgatgagcc
      721 gggaaggaga gatctgatgc cagggggaga atttcaagcc aaggtcaaac caggagcaga
      781 cagctcgatg atcgagctcg tcggcctggt cgaccgtggg gcgaaggagg aaatggagag
      841 ggcttaccag gaagctgtgc aggccccggg ggagatcctc ctcgacttca aggacgtgac
      901 atacatcaac tccaccggaa tcgcggtcat tgtcggagtg ctggcgatgg cgagggccga
      961 gaacaggaag gtgggggcac gcgggttgac cgaccactac aaagaagtct tcgagatcac
     1021 ccgtctcgcc gacttcatgc acatctacga ggacgcgaca gcggggaact gagcgaggac
     1081 catgcctaca gccaacctga ccatgaacat ccgggaagta tcagaaggaa tccgtgtgat
     1141 cgatgtcgtc ggtgagataa ccgccttctc cgagaaggag atatcagagg ctcacgagaa
     1201 ggcaggagac ggtgccacgg cagtgatcct gaacttcgaa gggctcgatt acatgaacag
     1261 tggcggcatc gggttgttgg tgacgaccct gattcgagcc cagcgcgccg gtcacgcact
     1321 tcgcgcctac ggtctgaccg agcactaccg agagatcttc aacctgacca gactcgacga
     1381 ggccatcggc atctacgacg acgaggccgc ggcgatcgcc ggctgatgaa agcggccgga
     1441 tccatcagtc gcatggtgga cattccggcc ctgattgaga ccttgcgagt gcacagtgtt
     1501 gcgctcagcg caacgttgtg cacagtcaat ccaacgccgc tcgtaccgcg tgatagccgc
     1561 acatcccgtg gaccccggcg ccgggaggtg tcgccgaaga gcagagatac acctccccac
     1621 cgagtgaata tggggactct gctccgatct gcaggagttt tcgcaatccg tactggccgc
     1681 cagagatgtc cccgcctatg tagttggggt tgtacgactg gtatccgatc ggaccacggg
     1741 tggctcgctg caagacgatg tccttgaagc cgggggcgaa ccgctccacc tgatcctcaa
     1801 tggccacggt catgtcgacg ttcgacccgt tggggacatg gcagtaagcc caagcggtgt
     1861 gtttgccatc gggagcccgg gaatcgtcga agagggactg ctgggcgagg agcacgaaag
     1921 gtcgcttcgg atggtcgcca cgagcaaccg ctcgttccga ggctgcgacc tcggcatacc
     1981 tgccgccgag atgaaccgtg gccgcttgtc cactcgatgg gtcggcccac ggaatcgggc
     2041 cgtcgagagc ccaatcgact ttgaagatgc ctggcccggg ctgccagcgt gagagttggc
     2101 ggaccttgcc cggagagata cgggtgtgag cgatttgata tgcggcaggg ggcatgacgt
     2161 ccaagaacac gggccccggt ggcaaatcgt cgaccgagtc gactgtgcgg cccagctcga
     2221 tcttcccacc gagctcttcg aatctggcgg ccagagcgtc ggtgatcgat cgagagccgc
     2281 ctcgggccaa gggccacccg tgactgtggg cgatggctcc gagcatgaga ccgactgctg
     2341 atgtggccag gccgtagaac ggcgcgatcg catgtgcggc gatgccggag aagagtccct
     2401 tcccctcctc ggtgtcgaac cgtttcgcca gtgcggcggc gggtagccca ccgaggagtc
     2461 ccagcctcgt gaaggccccg gggtggtcgg gaatcacctt gaccggtccc agggcaactc
     2521 tcacgtagtc ctcgatctgc tcaacgatcg gtgccatgag cttgcggtaa gactcttcgt
     2581 ccacaccgag accagaagcg gtctcatcga cggatcggcg cagcacggca accctgccgc
     2641 cgtcgagcgg atgtgtcaca gggatgggcg gattgaccca atccacctcg agcccgatct
     2701 cgttgaaata cggagaggcg atgccggcag ggtggatggc cgaacagaca tcatggagaa
     2761 atcccggaag tgtcagctcc tcggtccggg tcccaccgcc aatgtcgtcc gcagcctcga
     2821 tgagaagtgt cttccgacct gatcgggcca actcgacagc ggcagccagg ccgttgggcc
     2881 cggctccgac gaccgtggca tcgaaatcac tcatccgcct tgattgtcgc cgatcttcgt
     2941 tacgaaacgg tgaccccagg tctcgtaacg gtcggggaac ggttgccgcg tacacctgag
     3001 cactgaaagg agatcgcgat gaccgatccg agaaacgccg acaattgggc caagccgatc
     3061 gatgtcttcc acgtcgatga gaccatccag ggagtcaaca agggcaacgt ggaggggcga
     3121 aggcccacag gtccgctcca cggcttcggc cagctgtggc agaagtcgta cgagatctcc
     3181 atcccgggcc cgccgccaga ggaagtagtt caggcctgga aggacaactt cgcagagttg
     3241 tggtatccgg gtaacaagtt ctacgcccca gccggtggca ttgcgcccgg tgaagttgca
     3301 gtgatcgccg gcggtcgcgg tccgaccaag gtcaccagcg gggttcgagt catctacgcc
     3361 gacgagcggt cctggtcata catgacgcct gagggtcatc cgtgggcggc gatcatcacg
     3421 ttcagcacac acgacgagga ggatgggacg aaggctcgta tcgatctgat ggtccgggcg
     3481 aatgatccgc tctacgaggt gtcgttccac atctacacct cgagactcga ggacaagatc
     3541 tggacccaca tgctcacggc gttggctgcg agtttcgggt cgaagggcgg cgtcgtccaa
     3601 aggaccgcgc tgatcgacaa acgtcgccag tggcgggagt ggcgcaacat ctggaagaac
     3661 tcggcggttc ggacactgtt gcggcgcgac cgtcgctgag gatttgggcg aaagagcagg
     3721 ccccccgagt tcggggggcc tgtctcttgc tcgttcgtgg accagtgctt agagcagcac
     3781 agccttcttg cggatctgaa ggaacacgaa tcccactgct accagagtca atccggtgaa
     3841 gaaggcgaag gccgccaacg cataggacac gattccgagg ctcagggccg tggtcagtgt
     3901 ggttgcgttc agccatgagg ctcttgcctc attgggaacc atcaaagcct caccattctc
     3961 gtcgaggacg acctcgccgt tctcatccac ctgcgggacc atgcggtcca tctcggcgta
     4021 gtagagaccg tcggtgttct ccaactgatg atgggtgatg atgtcggcct gggcccacat
     4081 cgtgaacggc ccacggaccg cggtctccgg gatggccgca tcatcggggg tgacgacatt
     4141 ctgagcggct gcatggtccc atgtgtacca agcgccgaag atgccaccag ccatggatcc
     4201 gaatccgacg gccagggcca ggatcgcggt tgcgagcaag atgaagctct tcttgtaggg
     4261 gacatctgcc tccgggaggc ggacatctgg ggtgatggca gtcatttcgt gcctccttat
     4321 tgctctggta actgccttac accacgcaat atcgccgatt gtgaatgagt tcacgagggg
     4381 caaaggtccc agttgggagg ccggaagtcc ctggtggggt gggacccagg gaaggccgtt
     4441 acatcaggtg ccggtgtagt ccgggtcccg cttctccacg aaggcgctca tcgcctccat
     4501 gaggtcgttg gagatgagga acgaggagt
//
LOCUS       DASUTA010000423         3115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_386627, whole genome shotgun sequence.
ACCESSION   DASUTA010000423 DASUTA010000000
VERSION     DASUTA010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386627"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VFZ80_08435"
     CDS             complement(<1..1031)
                     /locus_tag="VFZ80_08435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX6287499.1"
                     /translation="MTRATRAIAVAIALITMIALPAGAAPFVFPVMDPDGFPVDTNPP
                     SITAASWILYDESTDMVLAEWDADTRRPMASITKIMTVMLALEKGNVDDEVVVSEVAA
                     GQGGQEMGLVAGETVTLGALVRAAMVRSGNDAAAAIAEHIGGSIEDFVTMMNERAAEL
                     GMADTNFVNPNGLDASGHYSSARDMLTLSKQAMSIPAFEELARSRALVFPDSPNGDQR
                     SATNTNRILNSYEGVIGVKTGETPNAGLTYVGAAERDGRRLYVVVFRSIGQRAHFADA
                     IALYDWAFHDLGIQGTLALGIPYEPVADRVDESPLQVEANVETLLHTAAQGITTKAPG
                     PSNEEEVSGP"
     gene            complement(1028..1705)
                     /locus_tag="VFZ80_08440"
     CDS             complement(1028..1705)
                     /locus_tag="VFZ80_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6287500.1"
                     /translation="MPEGAQLSIGEVINLLRDDFPDVSVSKIRFLESQGLIDPDRSGS
                     GYRQFDNNDVSRLRFILQQQRDHFLPLKVIKSKLTLWERGEDVSGDGGANQNNLNGAG
                     EPLDRAELLKRSRLEEGELSELIDIGLIRPIEDSVVFPPEAGIIAMEAKRLMDQGLEA
                     RHLRTIKHSSERESDLLKQLVAPLVKASNPEAKARAKDVLESSADSVQAMHKALMTSE
                     LRQHLKT"
     gene            complement(1698..2141)
                     /locus_tag="VFZ80_08445"
     CDS             complement(1698..2141)
                     /locus_tag="VFZ80_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX6287501.1"
                     /translation="MSQSGGAEGADKAEAEVEVETDPTVVYVPIADRADHPLTPEEAA
                     HVEGLTGFALVVERGPRTGMTFVLREGNTTVGRHPESDIFLNDVTVSRHHCRFVTTPD
                     TIAVEDSGSTNGTYVNDERVDSAELGAGDEVLVGRFHFVVAHGDA"
     gene            complement(2182..2562)
                     /gene="gcvH"
                     /locus_tag="VFZ80_08450"
     CDS             complement(2182..2562)
                     /gene="gcvH"
                     /locus_tag="VFZ80_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005150021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX6287502.1"
                     /translation="MNTPEDLLYTDEHEWVKRLGDGAVRIGVTDYAQDQLGDVVYVEL
                     PETGREVAKDDLVVEIESTKSVGEVYAPFSGTVTRVNEAVAETPELVNTSPYDEGWLV
                     EIEVSGDLSEEGLLDAAAYRELTE"
     gene            complement(2562..>3115)
                     /locus_tag="VFZ80_08455"
     CDS             complement(2562..>3115)
                     /locus_tag="VFZ80_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX6287503.1"
                     /translation="LVSFVRLAAIPVFWWLLLGADNVTAATILFALVATTDWIDGYLA
                     RRLNQVSKLGKSLDPVADRLMIASAVIAGLIADIVPSPIGIALIAREIYMAIVTLFVF
                     SRTKTTLEVRKLGKWATFLVYSAIGWFYIGAIPFLEVILIPLAWTAGVIGLVLYWVTA
                     YQYTRDALGVVGELESTASPEES"
BASE COUNT          573 a          992 c          925 g          625 t
ORIGIN      
        1 gggcctgaca cctcctcctc attcgacgga ccgggagctt tggtggtgat tccctgggca
       61 gcagtgtgca aaagcgtttc cacattggcc tcgacctgta atggcgactc gtcgacacga
      121 tccgcaaccg gttcgtaggg aatgccgagt gccaacgtgc cctgaatccc cagatcgtgg
      181 aatgcccagt catacagggc gattgcgtcg gcaaaatggg cccgctgccc gatggagcgg
      241 aacacgacga catagagccg acgtccgtct ctctccgccg cgccgacgta tgtgagcccg
      301 gcgttcgggg tctccccggt cttgacgcca atcacccctt cgtaggagtt gaggatccgg
      361 ttggtgttgg tcgccgagcg ttggtcgccg ttgggcgagt cgggaaatac gagggcgcgc
      421 gagcgagcca gctcctcgaa cgcgggtatc gacatcgctt gtttgctcag tgtcagcatg
      481 tcgcgggcac tggaatagtg gccactggcg tcgagaccgt tggggttgac gaagtttgtg
      541 tcggccatgc ccaactcggc cgcccgctcg ttcatcatgg tcacgaagtc ctcgatcgag
      601 ccaccaatgt gctctgcaat ggccgctgcg gcgtcgtttc ctgatctcac catcgccgcc
      661 ctcaccagcg ctccgagagt caccgtttcc ccggccacga gacccatctc ctgaccgccc
      721 tggccggcgg ccacctcgga gaccacgacc tcgtcgtcga cgttgccttt ctccagggcg
      781 agcatgaccg tcatgatctt cgttatggag gccattggcc tgcgagtgtc ggcgtcccac
      841 tcggccagca ccatgtcggt cgactcgtcg tagagaatcc aggaagccgc cgtgatcgaa
      901 ggcgggttgg tgtcgacggg gaacccgtca gggtccatga cagggaagac gaagggagcc
      961 gctcccgcgg ggagcgcgat catcgtgatg agggcgatcg ccacagcaat cgctcgagtt
     1021 gctctggtca tgtcttcaga tgctgtctca gttcggatgt catcaacgcc ttgtgcatcg
     1081 cttgcaccga gtccgcactc gactccagca cgtctttggc ccgcgccttg gcttcgggat
     1141 tggacgcctt caccaacggg gcgaccaact gcttgagcaa gtcggactcc cgctccgagg
     1201 agtgcttgat ggtgcgtaga tgccgcgcct ccaagccctg atccatcaga cgcttggcct
     1261 ccatggcgat gatcccagcc tcgggcggga acaccaccga atcctctatg ggccgaatca
     1321 agcctatgtc gatgagctcg cttagctcac cttcttcgag ccgggatctc ttcaacaact
     1381 ctgctcgatc taatggctca ccggcaccgt tgaggttgtt ctggttggca ccgccgtctc
     1441 cactcacgtc ctcgccccgc tcccacaggg tcaacttcga cttgatcacc ttgaggggca
     1501 ggaagtggtc tctttgctgt tggaggatga acctgagccg tgagacgtcg ttgttgtcga
     1561 actggcgata tccagagccc gaccgatcgg gatcgatcaa gccctgactc tccaggaatc
     1621 tgatcttcga gaccgagacg tcggggaagt catcgcgaag gaggttgatc acctcgccga
     1681 tgctcagctg agctccctca ggcatcaccg tgggccacta cgaagtggaa ccggcccacc
     1741 aggacttcgt ccccggcccc gagctcggca ctgtccacgc gttcgtcgtt gacataggtg
     1801 ccgttggtgg agccactgtc ctctacggcg atcgtgtcgg gagtcgtcac gaaccggcag
     1861 tgatgccggg agacagtcac gtcgttgagg aagatgtcgc tctccggatg tcggccgaca
     1921 gtggtgttcc cctctcggag aacgaacgtc atgccggttc tcggacccct ctcgacaacg
     1981 agcgcaaacc cggtcagacc ctcgacatgg gccgcttctt caggggtcaa gggatgatcc
     2041 gcccgatccg cgatcggaac gtagaccacc gttgggtcgg tctcgacctc aacctcagct
     2101 tcggccttgt cggcgccctc agcgccgcct gactggctca ttcccggaat gataggagga
     2161 tcgaagcagc gtccaggaga actactcggt cagctctcga taggccgctg cgtccagtag
     2221 gccctcctcg gagagatccc cactgacctc gatctcgacc aaccacccct cgtcgtaggg
     2281 actggtgttg accagctccg gtgtctcggc gacggcttcg ttgacacgag tcaccgtgcc
     2341 ggagaagggg gcatagacct cgccaaccga cttcgtgctc tcgatctcga ctaccagatc
     2401 gtccttggcc acctcgcgac cggtctccgg tagctccaca tacaccacgt ccccgagttg
     2461 atcctgggcg taatcggtga cgccgattcg gaccgctccg tctccgaggc gcttgaccca
     2521 ctcgtgctcg tcggtgtaaa gaaggtcttc cggtgtgttc atcaactctc ctcggggctg
     2581 gccgtcgact ctaattctcc cacgactccc agggcgtcac gtgtgtattg ataggcagtg
     2641 acccagtaga ggacaagacc gatgaccccg gccgtccacg ccaacgggat gaggatgacc
     2701 tcgaggaagg ggatggctcc gatgtagaac cagccgatcg cggagtacac gaggaaagtc
     2761 gcccatttcc ccagcttgcg cacctctagc gtcgtcttcg tgcgactgaa cacgaacagg
     2821 gtgacgattg ccatgtagat ctctcttgcg atgagcgcga ttccgatcgg ggacggcact
     2881 atgtctgcga tcagcccggc gatcacagcc gatgcgatca tgagcctgtc tgcgaccgga
     2941 tcgagcgact ttcccagctt gctcacctgg ttgaggcgtc gcgccaggta gccgtcgatc
     3001 cagtctgtgg tggctaccag ggcgaacagg atcgtcgccg cggtgacatt gtctgcaccg
     3061 agcagaagcc accagaacac cgggatggcg gccaaccgga caaacgaaac cagat
//
LOCUS       DASUTA010000424         2334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_256815, whole genome shotgun sequence.
ACCESSION   DASUTA010000424 DASUTA010000000
VERSION     DASUTA010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256815"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VFZ80_08460"
     CDS             complement(<1..585)
                     /locus_tag="VFZ80_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX6287504.1"
                     /translation="MKETERVEAPQGPGPRFMAGQLGQKAISFGPSLKRLVGHLRPER
                     VKVIFVVLTAVVSVGLAAFGPRVLGWATDIVFAGAIGASLPAGITQEQAIEGARAAGQ
                     TQIADLLTGVDFTPGVGIDFQALGLVLLLCVGLYLGAALFGYLQGYLLNDVVQGTIYR
                     LRSSVEDKLNRLPLSYFDRQPRGELLSRVTNDIDN"
     gene            complement(597..2330)
                     /locus_tag="VFZ80_08465"
     CDS             complement(597..2330)
                     /locus_tag="VFZ80_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018786108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6287505.1"
                     /translation="MLWKLLKQYLAVYRRELTIVISLQLIATIAALYLPTLNADIIDN
                     GVVRGDTDYIIRIGLVMLAVSFVQIGATIVAVYFGSRAAMGLGRDLRAGIFSKVAGFS
                     SQEVSSFGAPSLITRNTNDVQQVQMLTATGLTLMVMAPIMMVGGIIMALREDVGLSWL
                     LAVAVPILAGSVGFIASRLIPSFRRMQGRIDDVNRVLREQATGIRVLRAFVREPYETE
                     RFDETNTELTNVSLTVGRWMAAMFPIVMLILNLSSVAVLWFGGLRIEAGAMQIGSLTA
                     FLTYLLQILMSVMMATFMLVMIPRASVSADRIGEVLDSESTVRLAAHPVTELDRAGHL
                     DFENVTFRYPGAEYPVLSDVSASARPGQTLAIVGSTGAGKTTLTNLIPRLFDVTSGKI
                     RVDGVDVRDLDPEVLWSKIGLVPQKPYLFTGTVASNLRDGKPDATEEEMWAALEIAQA
                     REFVEAMPDGLESPIAQGGTNVSGGQRQRLQIARALIRRPEIYVFDDSFSALDLATDA
                     RLRRALRPVTRNSTVVIVAQRVSTITDADQIMVLEDGRVVGIGTHDELLTTCPTYVEI
                     VESQAEAKAAS"
BASE COUNT          432 a          711 c          774 g          417 t
ORIGIN      
        1 gttgtcgatg tcgttggtca cccggctcag cagttcgcct ctgggctggc ggtcgaagta
       61 gctgaggggg agacggttca gcttgtcctc cacgctggag cgaagcctgt agatcgtccc
      121 ttgcacgaca tcgttgagga gatagccctg gaggtagccg aacaacgctg ctcccagata
      181 gaggccgacg cagagaagca agaccagccc gagcgcctgg aagtcgattc cgacccctgg
      241 ggtgaagtcg actccagtca agaggtcggc gatctgagtt tgtccggctg cgcgtgcccc
      301 ttcgattgcc tgttcctggg tgatacccgc ggggaggctc gctccgattg caccagcgaa
      361 gacgatgtcg gtggcccagc ccaggacccg cggtccgaag gcggcgagcc cgacactgac
      421 cactgcggtc aggacgacga agatgacttt gacccgttcg ggtcggaggt gacccacgag
      481 acgtttgagc gaggggccga acgagatcgc cttctgcccg agctggccgg ccatgaatct
      541 cggtccgggg ccctgaggcg cctcgacccg ctcggtctcc ttcatggtac gcccgctcac
      601 gacgccgcct ttgcctcggc ctgcgactcg acgatctcca cgtaggttgg gcatgtcgtc
      661 agcaactcgt cgtgtgtgcc gatccccacc accctgccgt cctcgagaac catgatctgg
      721 tcggcgtctg tgatggtgga cacccgctgg gcaacgatca cgacggtcga gttcctggtc
      781 accggtctca gggcacgccg caacctggcg tcggtggcga ggtcgagagc ggagaacgag
      841 tcgtcgaaca cgtagatctc tggtcgtctg atgagggcac gggcgatctg cagacgctga
      901 cgctggccgc ccgacacgtt ggtcccgccc tgggcgattg gggactcgag cccgtctggc
      961 atggcttcga cgaattcgcg agcctgggcg atttccagag cggcccacat ttcctcttcg
     1021 gttgcatcgg gcttgccgtc gcgcaggttg gaagccaccg ttccggtgaa caagtagggc
     1081 ttctggggga ccaggccgat cttcgaccac aggacctcgg ggtccaggtc tctcacatcg
     1141 acaccgtcca ctcggatctt cccggaggtg acgtcgaata gtcgggggat gaggttggtg
     1201 agcgtggtct tcccggcacc ggtcgagcca actatggcca gggtctggcc aggtcgtgcg
     1261 ctggccgaga catcgctcaa gaccgggtac tcggcacccg gatagcggaa ggtcacgttc
     1321 tcgaagtcga gatggcctgc tctgtcgagc tcggtcaccg ggtgcgccgc caggcggacg
     1381 gtcgactcac tgtcgagcac ctcgccgatg cggtcggcgg acaccgatgc ccgcgggatc
     1441 atcacgagca tgaaggtggc catcatcacc gacatcagga tctggaggag ataggtcaag
     1501 aacgcggtca gagacccgat ttgcatggct ccggcctcga tgcggaggcc tccgaaccag
     1561 agcacggcca cgctggagag gttgaggatc aacatgacga tggggaacat ggccgccatc
     1621 cagcgtccta ccgtcaacga gacgttggtg agctcggtgt tggtttcgtc gaatcgctcg
     1681 gtctcgtacg gctcacggac gaaggcgcga aggacccgga tgccggtggc ctgctcgcgc
     1741 aacacacggt tcacgtcgtc gatgcgcccc tgcatcctcc ggaaggaagg gatgagccga
     1801 ctggcgatga aacccaccga gccggcgaga atcgggacgg cgactgcaag aagccaggag
     1861 aggccgacat cctcccgcaa ggccatgatg atcccgccga ccatcatgat cggcgccatc
     1921 accatcaggg tgagacccgt cgccgtgagc atctgcacct gttgcacgtc gttcgtgttc
     1981 cgggtgatca gcgacggggc tccgaatgag ctgacttcct ggctggagaa gccggcgacc
     2041 ttggagaaga tgccggcgcg caggtcgcgt cccaatccca tggccgcccg cgagccgaag
     2101 tagacggcga cgatggtggc cccgatctgc acgaacgaca ccgccagcat caccaatccg
     2161 attcgaatga tgtagtcggt gtcgcctcga accacgccgt tgtcgatgat gtcggcgttg
     2221 agtgtgggga gatatagggc cgcgatggtg gcgatcagct ggagcgagat gacgatcgtc
     2281 agctcacgcc ggtagacggc gagatactgc ttgaggagct tccagagcat catt
//
LOCUS       DASUTA010000425         3723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1162854, whole genome shotgun sequence.
ACCESSION   DASUTA010000425 DASUTA010000000
VERSION     DASUTA010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162854"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VFZ80_08470"
     CDS             complement(<1..650)
                     /locus_tag="VFZ80_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEX6287506.1"
                     /translation="MMPTWITDLFANFWVSLGGKLLLVALLIPLIGMVIGFAEMKLSA
                     KMQYRVGPYFAGGRWGWAQLIADGVKFFQKEDIIPTDADRPVFKTAPILVLVGTVALF
                     VVIPFGPNLIFRDLDVGIFYALAISSLSTIGVLMAGWSSANKYSLIGGLRAAGQLIAY
                     ELPLVLAVVGPVILAGTMSLQGIVQAQVDAGIPYIVFQVVAFVIFMIAALAELTRIP"
     gene            complement(810..2003)
                     /locus_tag="VFZ80_08475"
     CDS             complement(810..2003)
                     /locus_tag="VFZ80_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D 1"
                     /protein_id="HEX6287507.1"
                     /translation="MTTIDPEVIRHLSEASVSVEIETEDMTLNIGPQHPSTHGVLRLV
                     ARVDGERCFDVQPVIGYMHRGYEKLSEVRSYPQITTIVNRIDWVSGYANEVPFIVAAE
                     KLMELEVPERAQYIRLILTEMARISSHLVFNASYPLELGAATPLFFALRERERVLDLL
                     ESVTGGRFHPNFNRIGGVKPAYASGSNTRKLPQDLPAGFFAETRTAMDKVLEASEQLE
                     DLVAGNEIILARTKGVGFLPPEVAAAYGVSGPNLRASGVPFDLRKNQDYLPYDRFDFN
                     VITGENGDCWDRWHVRLLEIRESVKIVQQAIDGIPSGDLQAKVPKVIKVPAGETYVRA
                     ENPKGEMGYYLVSDGKSGPYRLKIRSASFSNLSILPWILEGALVPDIIAIMGSLDFVL
                     GDVDR"
     gene            complement(2000..2596)
                     /locus_tag="VFZ80_08480"
     CDS             complement(2000..2596)
                     /locus_tag="VFZ80_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX6287508.1"
                     /translation="MSSSPSETATHPLQEFADRVAEAVGGAAEIHFDTVKVRVSNDSW
                     IAAHATARDDLDLVFFSWLSAIDWTNQVEAGDPLDEEVEERIELMSALSDLTEGRLVV
                     FSTDLPSENPKIPSLVTTFAGANWHEREAHEMFGIEFEGHPNLIHLYLPDSFQGNPLR
                     KAFPLLSREVKPWPGKVDVEGMPEDPSPDEPSTENPEA"
     gene            complement(2577..3074)
                     /locus_tag="VFZ80_08485"
     CDS             complement(2577..3074)
                     /locus_tag="VFZ80_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX6287509.1"
                     /translation="MGLIQKFGAPKPVSWLLNWSRQYSLWLFQFGLACCAIELMAASG
                     PRYDFMRFGIIPLPASPRQADLMVVAGTVTDKMAPSIRRLYDQMPEPKYVISMGSCAN
                     CGGPYWDSYSVTKGVDQVIPVDVYVPGCPPRPEALMEGIVLLQKIIKDEDMKEKWNRR
                     ELEPV"
     gene            complement(3065..3430)
                     /locus_tag="VFZ80_08490"
     CDS             complement(3065..3430)
                     /locus_tag="VFZ80_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX6287510.1"
                     /translation="MSPYFESYLTVGAFAALGAVLVLVMLGVASVLRPVKPSQTKQMT
                     YECGVDPVGTGWSQTYVRYYVFGLLFVIFDVEAVFIFPWAIKVEELAYFGLIEMGAFI
                     TILVLGLVYAIRKGVLRWD"
     gene            3517..>3723
                     /locus_tag="VFZ80_08495"
     CDS             3517..>3723
                     /locus_tag="VFZ80_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287511.1"
                     /translation="MEIGPSYDGSGLVNLVSELESRMTGTSTFPRLVDASAIPEAATY
                     VLVLFDGLGVTQLDHADAGVFRSAL"
BASE COUNT          739 a         1120 c         1194 g          670 t
ORIGIN      
        1 gggatgcggg tcagctccgc cagagcggcg atcatgaaga tgacgaaggc gaccacctgg
       61 aagacgatgt atggaatccc cgcgtcgacc tgcgcctgga cgatgccctg gagactcatg
      121 gtgccggcga ggatcacggg cccgactacg gcgagcacca ggggaagctc gtaggcgatg
      181 agctgacccg ctgcgcgcag acccccgatg agggagtatt tgtttgccga cgaccagccc
      241 gccatcaata cgccgatcgt gctcagtgag gagatggcca gggcgtagaa gatgcccacg
      301 tcgaggtctc tgaagatgag gttggggccg aacggtatga ccacgaagag agccacggtc
      361 cccacgagga cgaggatcgg ggccgtcttg aagacgggcc ggtcggcgtc tgtgggaatg
      421 atgtcctcct tctggaagaa cttcacccca tcggcgatga gttgggccca gccccatctg
      481 cctccggcga aataggggcc cacccggtac tgcatcttgg ccgagagctt catctcggcg
      541 aacccgatca ccatcccgat gagggggatc aacagagcaa cgagaagcag cttgcctccg
      601 agtgagaccc agaagttggc gaagaggtcc gtgatccagg taggcatcat gatggcgccc
      661 gaccttgctc gacggcctgc caatccgaga gctcctcgcc agccggggca tcaaccctca
      721 gtctgggcct tgcaccgaag aaccccaagc aaacagacgc cggtcgggcg ctgctaccac
      781 tacgtgtcag ggtctgttcc atcttctacc tatctatcca cgtcgccaag gacgaagtcg
      841 agcgatccca tgatggcgat gatgtcgggc accagggctc cctcgaggat ccagggcagg
      901 atcgacaggt tggagaagct cgccgaccgg atcttcaatc ggtatggccc cgacttccca
      961 tcggagacca ggtagtagcc catctctccc ttggggttct ccgcgcgcac gtaggtctcg
     1021 cctgccggca ccttgatcac cttgggcacc ttggcctgga gatcgccgga aggtatgccg
     1081 tcgatggcct gctggacgat cttcaccgac tcccgtatct cgaggaggcg gacgtgccac
     1141 cgatcccaac agtcaccgtt ctcgcctgtg atcacattga agtcgaaacg gtcgtagggc
     1201 agatagtcct gattcttgcg cagatcgaac ggcacacccg aagcgcgcaa gttgggaccc
     1261 gacaccccgt aagcggctgc cacctcggga ggaaggaatc caacgccctt ggttcgagcc
     1321 aggatgatct cgttgccggc gaccaggtct tcgagctgct cactcgcttc gaggaccttg
     1381 tccatggcgg tgcgggtctc ggcgaagaac ccggcgggga gatcctgggg gagcttgcgg
     1441 gtgttggaac cagaggcgta ggcgggcttc accccgccaa ttcggttgaa gttggggtgg
     1501 aatcgaccac cggtgacact ctcgagcagg tcgagaacac gttcccgctc acggagagcg
     1561 aagaagaggg gtgtcgccgc acccagctcc agtgggtacg aggcgttgaa caccaggtgc
     1621 gatgaaatgc gggccatctc ggtgaggatc agcctgatgt actgggcgcg ctcgggaacc
     1681 tcgagctcca tgagcttctc ggcggccacg atgaacggga cctcgttggc gtagccggac
     1741 acccagtcga tgcggttgac gatcgtcgtg atctgcggat aggagcgaac ctcggagagc
     1801 ttctcgtagc cgcgatgcat gtagccgatc accggctgca cgtcgaaaca gcgctcgccg
     1861 tcgacgcggg ccacgagccg gagcacacca tgcgtcgagg gatgctgggg cccgatgttg
     1921 agcgtcatgt cctcggtctc gatctcgacg gagaccgacg cctcgctcag gtggcgaatg
     1981 acttcaggat cgatggtcgt catgcttcgg ggttctcggt ggacggctcg tcgggtgacg
     2041 gatcctcggg cataccctcg acgtcgacct tgcccggcca cggcttcacc tcgcggctca
     2101 ggagcgggaa ggccttgcgc agcgggttgc cctggaacga gtcgggaagg tagaggtgga
     2161 tcaggttggg atgcccctcg aactcgatgc cgaacatctc gtgcgcctcg cgctcgtgcc
     2221 agttggcacc ggcgaaggtg gtcaccaagg aaggaatctt tggattctcg ctggggaggt
     2281 ctgtggagaa cacgacgagt ctcccctcgg tcaggtcgga gagcgccgac atcagctcga
     2341 ttcgctcttc cacctcctcg tcgagggggt cgcccgcctc gacttggttg gtccagtcga
     2401 tggccgacag ccaggagaag aagacgagat cgaggtcgtc acgagccgtg gcatgggctg
     2461 cgatccaact gtcgttggag accctcacct tcacggtgtc gaagtggatc tccgctgcgc
     2521 cgccaacagc ctcggcgaca cgatcggcga actcctgaag cgggtgtgtg gcggtctcag
     2581 acgggctcga gctcacgccg gttccacttc tctttcatgt cctcgtcttt gatgatcttc
     2641 tggagcagca cgattccctc catgagggcc tcgggtcgcg gtgggcaacc cggcacgtac
     2701 acatcgaccg gaatcacctg atcgacgccc ttggtcaccg agtaggagtc ccaatagggc
     2761 ccgccgcaat tggcgcaaga ccccatcgag atgacgtatt tgggctcggg catctggtcg
     2821 tacagccgtc tgatcgaagg agccatcttg tcggtgacgg tgccggccac gaccatcagg
     2881 tcggcttgtc tcggggaagc agggaggggg atgatgccga accgcatgaa gtcgtaccgc
     2941 gggcccgacg ccgccatgag ctcgatggcg cagcaggcaa gaccgaattg gaagagccaa
     3001 agtgaatact gacgggacca gttgagcagc caagaaacag gcttgggagc cccgaacttc
     3061 tgtatcagtc ccatcgcaac acacccttgc ggattgcgta gacaagccca agaaccagga
     3121 tggtgatgaa ggcccccatc tcgatgagac cgaagtaggc gagctcctcg accttgatcg
     3181 cccaggggaa gatgaacacc gcctcgacgt cgaagatcac gaagagcagg ccgaagacgt
     3241 agtagcggac gtaggtctgg ctccatcccg ttcccaccgg gtcgacaccg cactcgtagg
     3301 tcatctgctt ggtctgggat ggcttgaccg ggcggaggac cgaggccact ccgagcatca
     3361 cgaggaccaa aacggcgcca agcgccgcga aggcgccaac agtcagatag ctctcaaagt
     3421 atgggctcat gcctcgcagg ttccggggga actgaccgct ggcgagtata cgcaccgagt
     3481 cttttacgtc gaagtcaggg ccctagcctg ctcgggatgg aaatcggccc ttcttacgac
     3541 ggttccggcc tggtcaatct ggtctcggaa ttggagtcga gaatgaccgg gacgtcgaca
     3601 tttccccggt tggtggacgc ttcggcgatc cccgaggccg ccacctacgt cctcgtgctc
     3661 ttcgacggtc tcggagtgac ccaactcgac cacgccgatg caggagtgtt tcgatcggct
     3721 ctt
//
LOCUS       DASUTA010000426         3158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1422739, whole genome shotgun sequence.
ACCESSION   DASUTA010000426 DASUTA010000000
VERSION     DASUTA010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422739"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="VFZ80_08500"
     CDS             <1..166
                     /locus_tag="VFZ80_08500"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HEX6287512.1"
                     /translation="GEEVKMWWNFVARTEEEISTAWGDWQSHNTDRFGEVPSRLARID
                     APRPPWLDHG"
     gene            200..1141
                     /locus_tag="VFZ80_08505"
     CDS             200..1141
                     /locus_tag="VFZ80_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008415638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX6287513.1"
                     /translation="MATGGTRAILAAMAANAGIAVAKFVAYLVTGSASMLAESIHSVA
                     DTSNQGLLLLGGHRAQRVADDRHQFGYGRERYFWAFVVAMVLFALGGVFSIYEGIEKI
                     RHPHEVESLGWAIGVLAFAIVAESLSFRTAIVESDRVRGRRSWWDFIRRSRSPELPVV
                     LLEDAGALIGLILALIGVGLAAITGDVLWDGVGTLSIGVLLVVISIVLTVEMKSLLIG
                     EAATDENVSALMSAVESVPSVNRVIDLKTQHIGPEELLVAGKLEFDHSLSNPQLSDAI
                     DEVETAIRHAVPLTSHIYLEPDVYDPGHPEEAEPQSH"
     gene            complement(1157..>3158)
                     /locus_tag="VFZ80_08510"
     CDS             complement(1157..>3158)
                     /locus_tag="VFZ80_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6287514.1"
                     /translation="EIRVCLDVLADHRLLTLTGPGGTGKTRMALEIARRCEADFGDGA
                     FFVGLASLSDPALVPMAILEVLGLKTASGVDPWEHLVRHLADRSTLLILDNFEQLLDG
                     AAVISNLLAECASLRVVVTSRSALRLLGEREFPVPPLEVPRTADPGEVQSSDAIRLFE
                     SRAKAVRPDFSLHEANLDVVREIVTSLDGLPLAIELAASRLRSITPDLLLERLDNRLL
                     ASRSADLPERQQTIVNTIGWSYDLLDERSKLLFEQLSVFSGTFGLTEAEQVCGPEADV
                     LDGLTGLVENSLLRQMAVAGEPRFRMLTVIREFAYAALVSRGADRPILDRHARVYMAL
                     AERADDEILTSSQLMWLSRLGDEHDNLRAAFDHSVSVGDADTALRLAGSLWRFWHITG
                     HLREGLQRTEVALGMGQESSPVARAHALTCQGGLLYWQGDWQATLAPYEEALSLFRQH
                     GSDAEVAEALYNACFPLVYLGQSDKATELLEESLALNERIGREIGVGRALWGLGNVAN
                     QNHDWAEAVANIERSVEIFSKLDAPFDLGWAWFMLAHSRYNMGETEQAIEPLGHALDV
                     FAEVNDVSALSLILDLVAAVTQQVGAESTAAYFVGASQRLKIDTGIEIGEVEMNRYTE
                     LQALDRRLRTEPTTEYEEGANASLPEVIERTRTELIRLLPSS"
BASE COUNT          597 a          981 c          994 g          586 t
ORIGIN      
        1 gggagaggaa gtcaagatgt ggtggaactt cgtcgcccgc accgaagaag agatctccac
       61 cgcctggggt gactggcaat ctcacaacac cgaccgcttc ggggaagtcc catctcggct
      121 cgcccgcatc gacgcccccc gccctccctg gctggaccac ggctgactcg cacttcccac
      181 cgcgcgcttt actgactcga tggcaacagg cggaacaagg gcaatcctgg cggccatggc
      241 ggcgaacgcc ggaatagcgg tggccaagtt cgtcgcctac ctggtgacgg gctccgcgtc
      301 gatgctcgcc gagtcgatcc actccgtcgc cgacacctcc aaccagggtc tgctgctgct
      361 cggaggtcac cgggcgcagc gggtggccga tgaccggcac caattcggtt acggccgcga
      421 gcgttacttc tgggcattcg ttgtggccat ggttctcttc gctctgggcg gggtcttctc
      481 catctacgag ggcatcgaga agatcaggca cccccacgag gtggagtcat tgggctgggc
      541 catcggggtg ttggctttcg ccatcgtcgc cgagagcctg agctttcgga cagccatagt
      601 ggagtcggac cgggtcaggg gccgcaggtc ttggtgggat ttcatcagga ggtcgcgttc
      661 gcccgagctt cctgtagtcc ttctggagga tgctggcgca ttgatcggcc tgatcttggc
      721 gctcatcggc gtgggactcg ccgccatcac cggcgatgtc ctttgggatg gggtcgggac
      781 cttgagcatc ggcgtcctcc tggtggtcat ctcgatcgtc ctcaccgtcg agatgaagtc
      841 gttgctgatc ggggaggcgg cgacggatga gaatgtgtca gcgctgatgt cggcggtgga
      901 gagcgtccct tcggtgaaca gagtgatcga cctcaagacg cagcacatcg gtcccgagga
      961 gttgctcgtc gccggcaagc tcgagttcga ccactccctg agcaacccac agctgagtga
     1021 cgcgatcgac gaggtcgaaa ccgcgatcag gcatgccgtt cccctgacct cgcatatcta
     1081 tctcgagccc gatgtctacg accctgggca tcccgaagag gcagagcccc agtcccattg
     1141 atcactcgac ccccggtcag ctcgagggca gaagacggat caactccgtt cgggttcgtt
     1201 cgatgacctc gggaagagag gcgttggctc cttcctcgta ctcggtggtg ggctcggtgc
     1261 gtaggcgtct atcgagagcc tgcaactccg tgtaacggtt catctcgact tcaccgatct
     1321 cgatcccggt gtcgatcttg agtctttgcg aggctcccac gaagtatgcg gcagtcgact
     1381 cggcaccgac ctgctgggtg acagcggcca ccagatcgag gatcagggac agtgccgaaa
     1441 cgtcgttgac ctctgcaaag acgtcgaggg catggcccag cggttctatg gcctgctcgg
     1501 tctcccccat gttgtagcga ctgtgggcaa gcatgaacca agcccacccc agatcgaagg
     1561 gtgcatccag cttcgagaag atctcgactg aacgttcgat attcgcgacg gcctctgccc
     1621 agtcgtgatt ctgattcgcg acattgccca ggccccaaag cgctcggccc acaccgatct
     1681 ctcgcccaat gcgctcattg agtgccagag attcctcgag tagctcggtc gccttgtcgc
     1741 tctggcccaa gtagacgagt gggaagcagg cgttgtaaag cgcttcagcc acctcggcgt
     1801 cgctcccatg ctgcctgaag agactcagcg cctcttcata gggcgcgagg gtggcctgcc
     1861 agtctccctg ccagtagagc aggccaccct gacaggtcag ggcatgcgcc ctcgcaactg
     1921 gagagctctc ctgtcccatc cccagcgcaa cctcggtgcg ttggagcccc tcacggagat
     1981 ggcccgtgat gtgccagaac cgccacagtg agccggccag ccggagtgcg gtgtcggcgt
     2041 cgccaaccga aaccgagtga tcgaaggcgg cgcgaagatt gtcatgctcg tccccaagtc
     2101 gcgatagcca catgagttga ctcgaagtga ggatctcgtc atcggccctc tcggcgagcg
     2161 ccatatatac cctggcatgt ctgtccagaa tcggtcggtc ggcccctctc gacaccaggg
     2221 cggcgtaggc gaattcacga atcaccgtga gcatccgaaa acgcggctcc cccgcaaccg
     2281 ccatctgacg aaggagactg ttctccacca aaccggtgag gccgtccagg acatcggcct
     2341 ccggcccgca gacctgttcg gcttcggtga gaccgaaagt gccactgaac acagagagct
     2401 gctcgaagag gagcttgctt cgctcatcga gaaggtcgta actccaaccg atggtgttga
     2461 cgatggtttg ctgtcgctcg ggaaggtcgg cggaacgaga ggcgagcagt cggttgtcga
     2521 gccgctccag gagtaggtcc ggtgtgatgc tgcgcagccg ggatgccgca agctcgatgg
     2581 ccagcggcaa accgtcgagc gaggtgacga tctcccgaac cacgtcgagg ttggcttcgt
     2641 ggagactgaa atcaggacgc actgctttgg ctcggctctc gaagagtcgt attgcgtcgg
     2701 aagactgaac ttcgcccggg tcggccgttc gtgggacttc gagcggaggc acagggaact
     2761 ctcgctcgcc gaggagccgc aacgccgacc tcgatgtgac cacaacgcgc aaagaggcac
     2821 actcggcaag cagattcgat atcactgcgg ctccgtcgag gagctgctcg aagttgtcga
     2881 ggatcagaag tgtcgaccgg tctgcgaggt ggcgcaccag gtgctcccag ggatcgactc
     2941 cagaagcggt cttgagccca agcacctcga ggatggccat cggcaccagt gccgggtccg
     3001 acagagacgc caggccgacg aaaaaggcgc cgtcgccgaa gtctgcctca caacgccggg
     3061 cgatctcgag agccatgcgg gtcttgccgg tgccaccagg cccggtcagg gtgagcagcc
     3121 gatgatccgc caggacgtcg agacatactc gaatctcg
//
LOCUS       DASUTA010000427         3163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1811455, whole genome shotgun sequence.
ACCESSION   DASUTA010000427 DASUTA010000000
VERSION     DASUTA010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811455"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFZ80_08515"
     CDS             <1..318
                     /locus_tag="VFZ80_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287515.1"
                     /translation="PIDEFLDESVRFAGEEWVASFNAVVADPMVSVIGPDDEMLGMED
                     PHSANNLRMLEAARGQGGTIHGVFLWDGSGGDGPGGTQHLASELIEAGGSVTIIDPNP
                     EAD"
     gene            415..861
                     /locus_tag="VFZ80_08520"
     CDS             415..861
                     /locus_tag="VFZ80_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287516.1"
                     /translation="MSNTKRSVSDGFSEAERAAMKARAEELKAQGRGGQKKAENESAV
                     LDAIAEMPDADRSLAERIHALVTANAPGLLPKTWYGMPAYANAEGKVVCFFKAAAKFD
                     GRYAVLGFEDAANLDEPPMWPVVFAIVEWTPAVEKKVAALVKTAAA"
     gene            complement(869..1069)
                     /locus_tag="VFZ80_08525"
     CDS             complement(869..1069)
                     /locus_tag="VFZ80_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287517.1"
                     /translation="MNPYNPGLERIASERIRQRMRDAEHSRLVALAKPANDRGHLFER
                     AGSAYFGLRARVARAFRRPRLA"
     gene            1383..1613
                     /locus_tag="VFZ80_08530"
     CDS             1383..1613
                     /locus_tag="VFZ80_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEX6287518.1"
                     /translation="MRFTNEAGWDRIARIVLGAVVLYLGWSGTVDGGLGDFLKVFGLV
                     PLATGIVGWCPIYAAFGFRTNAKRSSEPAATV"
     gene            1751..2560
                     /locus_tag="VFZ80_08535"
     CDS             1751..2560
                     /locus_tag="VFZ80_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6287519.1"
                     /translation="MPERPTASAFSSDLGNNSGHLYRFALTITRDPHLAADLVQDTFV
                     RALERSDQYQGEAPLRAWLRQILHNLAIDRARRAAREIVVADVEENWHDDAYTVDPAA
                     VAEGTLTREELEDGLARLPFIYRVVVILHDVEGWTVRQIAEVQDISLPAAKQRLRRGR
                     MSLVSALAEGHERRKQLEGVPMRCWDARQHVSDYLDGTLDEATAHLVEAHLEVCPTCP
                     PLYAALVGAHQHLGALRDPDAVIPTQLEQRIHSLLDGAGVDPGAETNRGPG"
     gene            2571..2888
                     /locus_tag="VFZ80_08540"
     CDS             2571..2888
                     /locus_tag="VFZ80_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012880047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287520.1"
                     /translation="MDDERRVSTVNKYWRLALAIIGLIAVIQELRKPAEERTWHGKVA
                     DFVPYDFRMPTLERVRSTYWNPEGPIIAGKVFGVGWAPNLGALNKLVGGWRDGTDSDR
                     QPA"
     gene            2924..>3163
                     /locus_tag="VFZ80_08545"
     CDS             2924..>3163
                     /locus_tag="VFZ80_08545"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX6287521.1"
                     /translation="MTSIRGTEEQGATFVELFFDLVFVFAVTQVTASLAHDLTSSGLL
                     RALIVFWLVWWAWTQYTWSLNEADTEHGWIRFITLL"
BASE COUNT          573 a          995 c         1028 g          567 t
ORIGIN      
        1 ccgatagacg agttcctcga cgagtcggtt cgtttcgccg gcgaggaatg ggtcgcctcg
       61 ttcaacgcgg tcgtcgctga cccgatggtg agtgtgatcg gccccgacga cgagatgctc
      121 ggtatggaag acccgcacag cgccaacaac ctgcggatgc tcgaggccgc ccgggggcag
      181 ggcgggacca tccacggtgt gtttctctgg gatggcagcg gtggtgacgg gcccggtggc
      241 acccaacacc tcgcctccga actgatcgag gccggcggaa gtgtcaccat catcgatccc
      301 aaccccgagg ccgattgacc cgcggcgatc atcagcgcga tcgagcagga atcgagaagc
      361 gtcttccccg tcgagcccgt agcttcacct gtgtcgccta tcgggagtcg agagatgagc
      421 aacaccaaga ggtccgtttc ggatggcttc tccgaggcgg aacgtgccgc catgaaggcc
      481 cgcgccgagg agttgaaggc gcagggccgc ggtggtcaga agaaggcaga gaacgagagc
      541 gccgtcctcg acgcgatcgc cgagatgccg gatgccgatc ggtctctcgc cgaacgtatc
      601 cacgccctcg tcaccgccaa cgcgccgggg cttctcccca agacctggta cggcatgccc
      661 gcctacgcca atgcggaggg aaaggtggtc tgcttcttca aggcggcggc caagttcgac
      721 ggccgctacg ccgtgttggg attcgaagat gccgccaacc tcgacgagcc cccgatgtgg
      781 cccgtcgtgt tcgccatcgt cgagtggacc cctgcggtcg agaagaaggt ggcggcactg
      841 gtcaagaccg ccgccgcctg agcgcggcct aggcgagacg gggtcgtctg aaggctcgag
      901 cgacccgggc tctgaggccg aagtacgcac tgcccgctct ttcgaaaagg tggcctcgat
      961 cgttcgccgg cttggccagg gcgacgagtc gggaatgctc cgcgtctcgc atcctctggc
     1021 gtatccgctc cgaggcgatt cgctccagcc cagggttgta cgggttcatg attcctccac
     1081 ggtttcttcc tgccagcccg gcagagttca ggtcgccgat tggcgaggtg tcgtgaacgt
     1141 atatcgaggg gatgatcccg tgaataggga tgattcccca gggacgatcc accaaaccga
     1201 gctccaacgc gacgatggcc gcgtgccgga ccgtgaccag gtcgcctctc gttcgccgac
     1261 attggggccg tcgcaccgcc aacgccctcc actccgaact cgaactcagc caacctgtat
     1321 ccctttgccc cacccgttcg tctctgatgt gactcggtca gcagacgaaa ggagaagcgg
     1381 aaatgagatt caccaacgag gcaggttggg atcggatcgc aaggatcgtt ctcggggcgg
     1441 tggtcctata cctgggatgg agcggcaccg tcgacggagg gctgggcgac ttcttgaaag
     1501 tgttcgggct cgttcctctg gccacgggga tagtgggttg gtgtccgata tacgccgcct
     1561 tcgggttccg caccaatgcc aagcggtcca gtgagccggc tgcaacggtc tgacggtcca
     1621 cggcatcggc gagggcgaag cggtcggtct cgaaggtcgg ccgtcttcgc tttctcgctt
     1681 ccggatgtca ggaatcagga cccgcttacc gtgagtgaga tgtcaaagcg acgacgtgaa
     1741 gaggtgtcgt atgcctgagc ggccgacagc gagcgccttc tcttctgacc tcggcaacaa
     1801 cagcggtcat ctgtaccgtt tcgccttgac tatcaccaga gatcctcacc tggcggctga
     1861 cctggtgcaa gacacgttcg tgcgggcgct ggaacgctcc gatcagtacc aaggggaggc
     1921 tccgcttcgc gcctggctcc gccagatact gcataatctg gcgatcgacc gtgcccggcg
     1981 agcggccagg gagatcgtcg tcgcggacgt cgaggagaat tggcacgacg atgcctacac
     2041 ggtcgatccg gcagccgttg ccgagggcac tctcactcga gaagagctcg aagatggcct
     2101 ggcccggctc cccttcatct atcgggtcgt cgtgatcctc cacgatgtcg agggatggac
     2161 ggttcgacag atcgcggagg ttcaggacat ctcgctaccc gccgccaaac agcgtctgcg
     2221 tcgtggtcgg atgtcactgg tcagcgcact ggccgaaggg cacgagcgac ggaagcaatt
     2281 ggaaggagtg ccgatgcgct gctgggacgc ccgacaacac gtctccgact atctcgatgg
     2341 caccctggat gaggcaaccg cacatctggt tgaagcccat ctcgaggtct gcccgacctg
     2401 ccctccgcta tatgcagccc tcgtcggagc gcaccagcac ctcggtgccc tccgtgatcc
     2461 tgatgcggtg atcccaactc aactcgaaca gcggattcat tcgttgcttg acggcgccgg
     2521 cgttgacccg ggcgcagaga cgaatcgcgg accggggtag cctgtgggac atggacgatg
     2581 aacgcagggt ctcgacggtc aacaagtact ggaggctggc gctcgcgatc atcgggctca
     2641 tcgcggtcat ccaagagctc cgcaagccgg ccgaggagag aacctggcac ggcaaggtcg
     2701 ccgacttcgt gccgtacgac ttccggatgc cgaccctcga gcgggtgcga agcacctatt
     2761 ggaaccccga aggtccgatc atcgcaggca aggtgttcgg tgtcggctgg gcccccaatc
     2821 tcggggcgtt gaacaagctc gtcggcggct ggcgcgacgg cacagactcg gaccgccaac
     2881 ccgcctgacc gggggtggag gccgccctta ggatcgggcg ggcgtgacca gcatccgagg
     2941 aaccgaagag cagggggcga ccttcgtcga gctcttcttc gatctggtct tcgtcttcgc
     3001 cgtcacccag gtcaccgcat ccctggctca cgatctgacc tcgtcggggc tgctccgcgc
     3061 attgatcgtc ttctggctcg tgtggtgggc gtggacccag tacacctggt cgctcaacga
     3121 ggccgacacc gagcacgggt ggatccggtt cataaccctg ctg
//
LOCUS       DASUTA010000428         2929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_1812007, whole genome shotgun sequence.
ACCESSION   DASUTA010000428 DASUTA010000000
VERSION     DASUTA010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812007"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VFZ80_08550"
     CDS             <1..433
                     /locus_tag="VFZ80_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX6287522.1"
                     /translation="VYLHLRPHTLKLGWTDSAVRRYVRDAGHLLDPLNELVRCDVTTA
                     NKRREKAIQSGIDELEERIAELSAKEELARLRAPIDGNQVMTYLGMSPGPAVGEIMDI
                     LLEKRIDDGPYSPLVAFEIARGWALERGMADPGPPPVSEEE"
     gene            465..1343
                     /locus_tag="VFZ80_08555"
     CDS             465..1343
                     /locus_tag="VFZ80_08555"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009810405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018786 - haloalkane dehalogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HEX6287523.1"
                     /translation="MLRRKFDPRKKKTAQVLGSTMAYVEHGSGDPIVLLHGNPTSSYL
                     WRDVIPELSDLGRCIAPDLIGMGDSARIAAGRDAYRFTMHAEYLEALLEVLEVTGNVT
                     LVGHDWGGALLFDWGRKNPEAVKGVAFMETILTPLTWDDWPKAAVGIFQGMRSDAGEE
                     MVLTKNVFVERILPGSVLNPIAERDMDVYRRPYVEPGESRRPTLTWPREIPIDGQPEY
                     VEGIVAANEAWLSQEEIPKLFVNADPGSIVGDRVRRVVRSWPNVTEVTVPGLHFIQED
                     SGTAIGIAIADWMRAL"
     gene            1372..2304
                     /locus_tag="VFZ80_08560"
     CDS             1372..2304
                     /locus_tag="VFZ80_08560"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX6287524.1"
                     /translation="MIVVAVPAVAMAIVSLAAFLGKWVWWLDVLANFRAQFVVALAIF
                     GLVIMMSKWRKTGYFVLAVGVVNLVVVLPLYIGSPAEASVDTSSIRVMSFNLLSTNEE
                     YSSVIEYIETVDPDLVLLHEASRPWEVALESAGLDYQIVRPRSDDLIFGTLVLIRGDS
                     VEAVSYGYASSSPRAVELTYVPEGWTTPLSVLGTHPLAPTDEERADLRDAQLGFAGQW
                     ASGQSGAFMVVGDFNSTPWSWPFRRLMGAADLHNSQTGFGLQPTFSTESNLLLRIPID
                     HLLHSDALEVTDRQLGPALGSDHFPLLVDLQLAG"
     gene            2366..>2929
                     /locus_tag="VFZ80_08565"
     CDS             2366..>2929
                     /locus_tag="VFZ80_08565"
                     /inference="COORDINATES: protein motif:HMM:NF017046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEX6287525.1"
                     /translation="MSNRYAEARRRLAEVVGPDGLAVIPAAHEVIRNYDVTHPFRQDS
                     FFWYLTGFDEPESVAVIAPGHDDGDFTLFVRPKDPSLEVWTGIRAGTEGAKETYGADA
                     AYELAELDDVLERMMHGREVLWYTTGNDRFDDRIASIVKSARAHRERMGGVVPSTVRD
                     VSVPLGEMILYKSAEEAESLRSACSLTA"
BASE COUNT          500 a          937 c          961 g          531 t
ORIGIN      
        1 cgtctacctc catctacggc cccacactct gaagctgggc tggacagact cggcggtgcg
       61 gcgctacgtc cgtgacgccg ggcatctgct cgaccccctc aacgaactgg tgcgctgcga
      121 cgtgaccacc gccaacaaga ggagggagaa ggcgatacag agcgggatag acgagctcga
      181 agagcgcatc gccgagctct ccgccaagga ggagctggcc agactgcgcg ccccgatcga
      241 cggcaatcag gtgatgacct acctcgggat gagcccaggg ccggcggtgg gagagatcat
      301 ggacatcctt ctcgagaagc gtatcgacga tgggccctat tcaccgttgg tcgccttcga
      361 gatcgcccgg ggttgggcac tggagcgcgg gatggccgat ccaggccctc ctccggtctc
      421 cgaggaggag taggcgccct ccgacgcgcc ataatcgggg tcacatgctg cgacgcaagt
      481 tcgatccccg gaagaagaag actgcacagg ttctcgggtc gacgatggcc tatgtcgagc
      541 acgggtccgg agacccgatc gtcctcctcc acggcaaccc gacgtcgtcg tacctgtgga
      601 gagacgtgat accggagctg tccgacctcg gcaggtgcat cgctcccgac ctgatcggca
      661 tgggcgattc ggcccggatc gccgccggca gggatgccta caggttcacg atgcatgccg
      721 aatacctgga ggctctgctg gaggtgctcg aggtgaccgg caacgtcacg ctggtcggtc
      781 acgactgggg cggggcgctt ctcttcgact gggggcggaa gaacccggag gcggtgaagg
      841 gcgttgcctt catggagacg atcctcaccc ctctgacctg ggacgactgg ccgaaggcgg
      901 cggtggggat attccaggga atgcgatcgg acgccggcga ggagatggtg ttgacgaaga
      961 acgtcttcgt cgagcggata ctgccgggct cggtgctcaa ccccatcgcc gagagggaca
     1021 tggacgtgta tcggcgccca tacgtcgagc cgggcgagtc gcgccgaccc actctgactt
     1081 ggccccgtga gatccccatc gatggccaac ccgagtacgt cgagggcatc gtcgcggcca
     1141 acgaggcctg gctgtcacag gaagagatcc caaagctctt cgtcaacgcc gatccaggct
     1201 cgatcgtcgg cgaccgggtg cgacgggtgg tgcgctcctg gcccaacgtc accgaggtca
     1261 ccgtgcccgg cctgcatttc atccaggagg actcagggac cgcgatcgga atcgcgattg
     1321 ccgactggat gcgggctctc tgagtccccg taggtcccta acctccactt cgtgatcgtt
     1381 gtagcggttc cggcggtcgc catggcgatc gtctcgttgg cggcgttcct tgggaaatgg
     1441 gtgtggtggc tggatgtcct ggcgaacttc agggcccagt tcgtggtggc cctggccatc
     1501 ttcggtctcg tcatcatgat gtcgaaatgg cgcaagaccg gctacttcgt ccttgccgtg
     1561 ggcgtggtca acctcgtcgt ggtccttccc ctctacatcg gctcgcctgc cgaggcgtcg
     1621 gtggacacgt cctcgatccg ggtgatgtcg ttcaacctcc tctccacaaa cgaggagtac
     1681 tcgagtgtca tcgagtacat cgagacagtg gatccggatc tggtcctgct ccacgaagcg
     1741 agtcgaccat gggaggtggc gctggaatcg gccggtctcg actaccagat cgtgcggcca
     1801 cgctccgacg atctcatctt cgggaccctg gtgctgatcc ggggagactc ggtggaggcc
     1861 gtctcctatg ggtatgcctc gtcgagccca cgcgccgtcg agttgaccta tgtgcccgag
     1921 ggttggacga cgccgctctc ggtccttgga acccatccgc tcgcccccac cgatgaggag
     1981 agagccgacc tgcgtgatgc ccaactggga ttcgccgggc agtgggcgtc cggccagtca
     2041 ggtgcattca tggtcgtcgg cgacttcaac tccacgccct ggtcgtggcc cttccggaga
     2101 ctgatgggag cagccgacct ccacaacagc cagaccggct tcgggctgca gcccaccttc
     2161 tctaccgagt cgaacctctt gctccggatc ccgatcgatc atcttctcca ctccgatgca
     2221 ctagaggtga cggaccgtca gctgggcccg gccctgggct cggaccactt cccgctgctg
     2281 gtcgatctcc aactcgccgg ctgacccgag gagcggcctg gtgccgccgg atgcggtggt
     2341 caccgtcgga attacgatcg gtggcatgtc gaatcgttat gcagaggctc ggcgccgtct
     2401 cgccgaggtg gtcgggcccg atggtctcgc agtgatccca gcagcccacg aggtcatccg
     2461 gaactacgac gtgacccacc ccttccgcca ggactccttc ttctggtatc tgaccgggtt
     2521 cgacgagccg gagtcggtgg ccgtgatcgc tcccgggcac gacgatggtg acttcaccct
     2581 cttcgttcgt cccaaggatc cgagcctcga ggtgtggacc ggcatcaggg ccggcaccga
     2641 aggggccaag gagacctacg gggccgatgc cgcctacgag ctcgcagagc tggacgacgt
     2701 cctcgagcgg atgatgcacg gccgggaggt gctctggtac acgaccggca acgaccgttt
     2761 cgacgacagg atcgcttcga tcgtcaagag cgcccgtgcc catcgggagc ggatgggagg
     2821 tgtcgtcccc tccaccgtgc gcgacgtctc ggtacccctc ggggagatga tcctgtacaa
     2881 gagcgccgag gaggccgaga gtctccgcag cgcctgctcg ttgacggcc
//
LOCUS       DASUTA010000429         3491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_903457, whole genome shotgun sequence.
ACCESSION   DASUTA010000429 DASUTA010000000
VERSION     DASUTA010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903457"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..1033)
                     /gene="lgt"
                     /locus_tag="VFZ80_08570"
     CDS             complement(209..1033)
                     /gene="lgt"
                     /locus_tag="VFZ80_08570"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX6287526.1"
                     /translation="MIGVLLASIPSPPVNDFHIGPLRVTFYGILVMAGVVVAWLITRN
                     RFVARGGDAVLAERIIIRVVIWGFVGARLAYISTHLDRFADEWWKVIAIWEGGIAIYG
                     GLTAGAIALVYYARKWGASLPDLLDSVAPAVPAAQAIGRWGNYFNQELFGTPTDLPWG
                     LEIDPQNRPAAFPDAETFHPTFLYESILNVILVFVIIWVGNRFPSMRGRLIGVYFVGY
                     GVIRFLMELIRTDTTFRFIGLSRNGWVSIAVIILGLLVLFWKRVKVEDDHYVLVKG"
     gene            complement(1030..1830)
                     /locus_tag="VFZ80_08575"
     CDS             complement(1030..1830)
                     /locus_tag="VFZ80_08575"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX6287527.1"
                     /translation="MISWWCTALARPWSWEWIPYPGIWLASILPVFLYVRAVRRHPEP
                     IDVRRLGLFVAGMAVFWLASDWPLGTLGAGYLASAHMTQFLLYTLVSAPLLLLGTPEW
                     LARSVLDRLRLRSTVVRLGGALVASGLIYNVILLTTHAPGTVDVLRNSQFGSFAMDVA
                     WMVAGLVLWLPVISPLPEGRATSAFAKMAYLFVTTSVVAVIPASFLTFTSTPIYAIYE
                     LAPRIGSLTAIEDQQLAGIIMKIATIPVIWSTIAVLWFRWTARETQPA"
     gene            complement(1827..2186)
                     /locus_tag="VFZ80_08580"
     CDS             complement(1827..2186)
                     /locus_tag="VFZ80_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX6287528.1"
                     /translation="MRPATARSALGMALVLVLAACGDDGSTDGLSGSELATEIGCFAC
                     HTDSDTDLAPTLHGIWGTEVELEDGTRVVVDETYVRRSITDPGADVVEGFDARMPIFG
                     LSESEVDRLVDYVRSLG"
     gene            complement(2167..2631)
                     /locus_tag="VFZ80_08585"
     CDS             complement(2167..2631)
                     /locus_tag="VFZ80_08585"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX6287529.1"
                     /translation="MVSRIGGASWIVLLLLIAACGEPGDGLVVTDARLGMPTGPNAAL
                     YFTVENHADGADVLESVATEVATSVEVHETVAGDDGTMGMRPVDAPLEVPADGSLTFE
                     PGGFHLMLVDVERLDVGDEIEVTLTWQNAGEMTIDAMVVEPQDVSEHEAGDG"
     gene            complement(2633..3238)
                     /locus_tag="VFZ80_08590"
     CDS             complement(2633..3238)
                     /locus_tag="VFZ80_08590"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX6287530.1"
                     /translation="MPRNAHRALLLPLIGSLLVAACGGAASTEDLQGVVIDPPQPKPE
                     FTLTDTSGDPYDFAAETEGKLALLYFGYLNCPDICPVHLAQIAETFDRIPNVARDAEV
                     VFVSVDPERDSPGEIRAFLDNFDERFIGLTGTLEELEAAQTAAGVPVAVFVGDGDEYT
                     VNHAAQVIAFAPDGLNHSVYPFGIRQSQWNNDLQVLAELGS"
     gene            complement(3272..>3491)
                     /locus_tag="VFZ80_08595"
     CDS             complement(3272..>3491)
                     /locus_tag="VFZ80_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6287531.1"
                     /translation="PFFIQWDVAEELHPGRVSVSHPSGKAALTSVAILGDHVDELTEW
                     APQPEGLTYLDGPYREITYHLAVAAQAL"
BASE COUNT          713 a         1119 c         1089 g          570 t
ORIGIN      
        1 gatgccgcca gtgtcgaggg cgaggactgg tccgctcagt ggagccctgt cgggcatgtg
       61 acgggacccg catcgatagg tcctgaactc gtcctcatca aatctggccg ttcggagaac
      121 tagagggaga agccggcgcc actcgagaca gatgaggtct ctggttcaag ccccggtggg
      181 cccaatcgaa caccccgtcc ctattcgctc agcccttgac caggacgtag tggtcgtcct
      241 cgaccttcac ccgcttccag aacaggacta agagcccgag gatgatcaca gcgatcgaga
      301 cccacccgtt acgggacagc ccgatgaacc ggaaggtggt atcggttcgg atcagctcca
      361 tcaggaacct gatcacgcca tagcccacga agtagacacc gatgagccga ccccgcatcg
      421 agggaaatcg gttccccacc cagatgatga cgaaaacgag gatcacgttg aggatcgact
      481 catagaggaa cgtcgggtga aaggtctccg catcggggaa ggcggcaggc cggttctgcg
      541 ggtcgatctc gagaccccag gggagatcgg tcggtgtgcc gaacagctcc tgattgaagt
      601 agttgcccca acgcccgatg gcctgggcgg ccgggactgc tggcgcaacc gagtcgagaa
      661 gatcggggag actcgccccc cacttcctgg cgtaatagac caacgcgatc gcaccggcgg
      721 tgagaccgcc atagatggcg atgcccccct cccaaatggc tatcaccttc caccactcgt
      781 cggcgaagcg atcgaggtgt gtcgagatat aggcgagcct ggcgccgacg aacccccaga
      841 tgacgacgcg aatgatgatt cgctcggcga ggactgcgtc gccaccacga gccacgaatc
      901 gattgcgggt gatcagccat gccacgacga ccccggccat caccaggatg ccgtagaagg
      961 tcactcgtag cggaccgata tggaaatcgt tcaccggtgg tgacgggatc gatgcgagga
     1021 ggacgccgat catgccggct gagtctccct cgccgtccac cggaaccaca aaacggcgat
     1081 cgtggaccag atcacgggga tggtggcgat cttcatgatg atccccgcca gttgttgatc
     1141 ctcgatcgcc gtcaacgacc cgatccgggg ggccagctcg tagatggcgt agatcggagt
     1201 gctggtgaaa gtgaggaagc tggcggggat gacggcgacg acgctggtgg tgacgaacag
     1261 ataggccatc ttggcaaacg ccgacgtcgc tcgaccttcg ggcagtggtg agatcacggg
     1321 cagccaaagg accaacccgg ccaccatcca cgccacgtcc atggcgaacg agccgaactg
     1381 gctgttcctc aagacgtcga ccgtgcccgg ggcatgggtg gtcaggagga tgacgttgta
     1441 gatgagtccg gacgccacca aggcgccgcc caatcgaacc actgtcgatc gcagacgcag
     1501 acgatcgaga accgatcgcg ccagccactc aggtgtgccc aagaggagga gtggcgccga
     1561 caccagcgtg tagaggagga actgggtcat gtgggcggag gccaggtacc cggcgcccag
     1621 agttcccagc ggccagtcgg atgccagcca gaacactgcc attccggcca cgaacaggcc
     1681 aagcctccgg acgtcgatcg gctccggatg tcgccgcacc gcccgcacgt agaggaacac
     1741 cggcaagatg gaggccagcc agatgcctgg ataagggatc cactcccagc tccagggacg
     1801 agccagagcg gtgcaccacc agctgatcaa cccaggctcc tgacgtaatc gacgagacgg
     1861 tccacctcgg actcgctcag cccgaagatc ggcatgcggg cgtcgaatcc ctcgactacg
     1921 tccgcgccgg gatcggtgat cgacctgcgc acatatgtct cgtctaccac aaccctggtc
     1981 ccgtcctcga gctcgacctc ggttccccag atgccgtgaa gcgtaggagc cagatcggtg
     2041 tccgagtcgg tgtgacaggc gaaacaaccg atctccgttg ccagttcgct gcccgatagg
     2101 ccatcggtcg agccgtcgtc gccacaggct gccaggacca acaccagggc catccccagc
     2161 gccgacctag ccgtcgccgg cctcatgctc cgatacatcc tggggctcga cgaccatggc
     2221 gtcgatggtc atctctccgg cgttttgcca ggtcagggtg acctcgatct cgtcacccac
     2281 gtcgagacgc tccacgtcta ccagcatcag gtgaaaccca ccaggctcga acgtcaggct
     2341 cccatcggcg gggacctcca acggagcatc cacgggccgc atccccatcg tcccgtcgtc
     2401 gccggcgacg gtctcatgga cctcaacgga ggtggcgacc tccgttgcga cactctcgag
     2461 cacatctgcg ccgtctgcgt ggttctcgac ggtgaagtag agagcggcgt tgggcccggt
     2521 cggcatcccg agacgggcgt cggtgacgac tagcccgtcc cctggctcgc cacaagcggc
     2581 gatcagcaac aagaggacga tccacgatgc gccgccgatc ctcgagacca tctcaactcc
     2641 ccagctcggc gaggacctgg aggtcgttgt tccactggct ctgtctgata ccgaacggat
     2701 agacactgtg attcagaccg tcgggagcga aagcgatcac ctgggcggca tgattgaccg
     2761 tgtattcgtc cccgtcaccc acgaagacgg caacgggtac cccggccgcc gtttgtgccg
     2821 cttcgagctc ttcgagcgtg ccggtcagtc cgatgaacct ctcatcgaag ttgtccagaa
     2881 aggccctgat ctccccgggc gagtcgcgct cggggtcgac cgagacgaac acgacctcgg
     2941 catcccgggc cacgttgggg atgcgatcga aggtctccgc tatctgggcc aggtgaaccg
     3001 ggcagatgtc ggggcaattg agatatccga aatagagaag cgccagcttg ccctcggtct
     3061 ccgccgcgaa gtcgtaaggg tcgccggagg tgtcggtgag ggtgaactct ggcttgggct
     3121 ggggtgggtc gatcaccacg ccctgcagat cctctgtcga ggcggcaccg ccacaggcgg
     3181 caaccaggag cgacccgatc agtggcaata gaagtgctcg atgagcgttt ctgggcacga
     3241 cggctccatg gtggcccttt tcggcgtcta cctacaacgc ctgagcagcg accgcgaggt
     3301 gataggtgat ctcccgatac gggccgtcga gataggtgag cccctccggt tgcggtgccc
     3361 actcggtcaa ttcgtcgaca tggtcaccga gtatcgccac cgatgtcagc gccgccttcc
     3421 ccgacgggtg gctcaccgag actcgccccg gatgcaactc ctcggccacg tcccattgga
     3481 tgaagaaggg c
//
LOCUS       DASUTA010000430         2559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1750
            k141_647763, whole genome shotgun sequence.
ACCESSION   DASUTA010000430 DASUTA010000000
VERSION     DASUTA010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961084
            Sequence Read Archive: SRR25158397
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 16:57:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,720
            CDSs (total)                      :: 1,696
            Genes (coding)                    :: 1,692
            CDSs (with protein)               :: 1,692
            Genes (RNA)                       :: 24
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647763"
                     /isolate="SMAG_U1750"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="38.99638889 N 117.1397222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..>2559)
                     /locus_tag="VFZ80_08600"
     CDS             complement(217..>2559)
                     /locus_tag="VFZ80_08600"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX6287532.1"
                     /translation="EVLAELHDGRNSEPIPYDQIPEVMVNAMLAAEDADFFEHDGIDF
                     SAIASAALDNFISDQTRGGSTITQQVVKNAFVGNEVSIRRKINEAFVSAELERRFPKE
                     RILEYYMNSVYFGAGAYGVKTAAEEFFNKKLSELQIHEAAALAVLVRNPSLYNPRRRP
                     EVTIERRDQVIAQMLEEGWITQAQATRATNQPLGVVDTPPRRGLADHVVAEVRRQLLN
                     SPEFAFLGDTNEERKRAIFGCPADDVACEGGGGLQIYTTIDLRLQQEANRILSEWLPL
                     LPYEENFAKCADIFDDFDENQDFYATYAETHSCTPTGAIAMVDNKSGAVKVMASGLDF
                     QFSQFDLAIQGRRNPGSAFKAIGLAAALEQGITMGNRYNGQSPYEIECEFVCSPDGTN
                     VWTVSNASGSSTGVISVEEATYRSVNTVYAQISRDTGPENIVDMARRMGITDSAVEPV
                     LSIVLGSSAISPLEMASAYSNFATNGLHADDYIVARIVNSEGEVIYEKEPEVEQVADA
                     AIFAAARRALVKVPTSSGTAPNADIDHPQGGKTGTHQSYLDAWYVGFTPEYSTAVWMG
                     YEAEQIPLEDLVIKGQSYSRVFGGSIPAPIWAEFMTIVTEGLPETQFPPEPDNIEEYL
                     VPPKTDVPNVISLEEGAAVNDLREAKLNVAVAEIASLEPEGTVVRQSIGGGATVDQGT
                     TVTIWVSNGEVPVASLPRFTDMTFEEAVALAEEFTLETNVQLSLVQEEYELSDPSRVG
                     IIVRTNPRPGTQITESATVVVFVGVLSQGNSNGNGGGGDG"
BASE COUNT          443 a          765 c          830 g          521 t
ORIGIN      
        1 cgggtcaggt cgtcggcggt ctcctccgat agccaggcat ctccatcgct gagcagggac
       61 cggtggagag ccttgtcgat ggccaggtcg acctctgtga tgggatcacc accactcttg
      121 gtcatctcga ggatcgacga acggtcatac atagcaaaga tctcagcggc ccgatctagg
      181 ccgacttcga tccgcgtgag cagagctccc gcgtctctac ccgtcaccgc cgccgccgtt
      241 tccgttgctg ttgccttgtg aaaggacacc gacgaaaacg acgacggtgg cagactcggt
      301 gatctgagtg ccaggtcttg ggttggttct cacgatgatt ccgacccgac tgggatcgct
      361 cagttcgtac tcctcctgaa ccaagctgag ctggacgttg gtctcgaggg tgaattcctc
      421 agccaaagcg acggcttcct cgaaggtcat gtcggtgaaa cgcggcagcg atgccaccgg
      481 gacctcgccg ttggagaccc agatcgtcac cgtcgtcccc tgatcgacgg tggcaccgcc
      541 accgatggac tggcgaacaa ctgtcccctc cggctccaaa gaggcgatct ccgcgacagc
      601 cacgttgagc ttggcttctc ggaggtcgtt gaccgctgct ccctcctcca ggcttatgac
      661 gttcggcacg tcggtcttgg gcggaacgag gtattcctcg atgttgtcgg gttcgggtgg
      721 gaactgagtc tcgggcaacc cctctgtgac gatcgtcatg aactcggccc aaatcggtgc
      781 cgggatcgat cctccgaaga cccgggaata cgactgacct ttgatcacca ggtcttccag
      841 cgggatctgc tccgcctcgt atcccatcca caccgcggtc gaatattcgg gtgtgaagcc
      901 gacgtaccag gcgtcgaggt agctctgatg cgtgcccgtc ttacctccct ggggatgatc
      961 gatgtcggcg ttgggtgctg ttcccgagct ggtcggcacc ttgaccagag cacgcctggc
     1021 agcagcgaag atggcggcgt cggcgacctg ttcgacctca ggttccttct cgtagatgac
     1081 ttcaccttcg gagttgacga tgcgggcaac gatgtagtcg tcggcgtgga gaccgttcgt
     1141 ggcgaagttg gagtaggccg acgccatctc cagcggagat atcgccgagg agccgagaac
     1201 gatcgagagc acaggctcga cagccgaatc ggtgatcccc atgcgtctcg ccatgtccac
     1261 gatgttctcc ggacctgtgt cccgcgagat ctgggcatag accgtgttca cagagcgata
     1321 ggtggcttcc tcgacactga tcactccggt cgagctccca gaggcgttgg agacggtcca
     1381 gacgttggtc ccgtcggggg agcagacgaa ctcgcactcg atctcgtacg gcgactggcc
     1441 gttgtagcgg ttacccatcg tgatgccctg ctccagggcg gcggccaggc cgatggcctt
     1501 gaatgcggag ccggggtttc ggcgaccctg gatggccagg tcgaactggc tgaactggaa
     1561 gtcgagcccc gaggccatca ctttcaccgc gcccgatttg ttgtccacca tggcgatggc
     1621 gccggttgga gtgcaggagt gtgtctcggc gtaggtggca tagaagtcct ggttctcgtc
     1681 gaagtcgtcg aagatgtcgg cgcacttggc gaagttctct tcgtagggaa gcagcggcag
     1741 ccactcggag aggatccggt tcgcttcctg ttgcagccgc aggtcgatgg tggtgtagat
     1801 ctgcaggcca ccaccgccct cacaggcgac gtcgtcggcg ggacaaccga agatggcacg
     1861 tttcctctct tcgttggtgt caccgaggaa ggcgaactca ggtgagttga gcagctggcg
     1921 gcgcacctcg gcgacgacgt ggtcggcgag accgcgccgt ggtggggtat ccacgacacc
     1981 gaggggctgg ttggtggcgc gggtggcctg ggcctgggtg atccaaccct cctcgagcat
     2041 ctgtgcgatc acctggtcgc ggcgctcgat ggtgacctca ggacgccgcc gcgggttgta
     2101 gagcgagggg ttgcgaacca gcaccgccaa ggctgccgcc tcgtggatct gcagctcgct
     2161 caactttttg ttgaagaact cctccgccgc cgtcttgacc ccgtaggcgc cggccccgaa
     2221 gtacaccgag ttcatgtagt actcgaggat gcgctctttg gggaagcgtc gctccaactc
     2281 ggccgacacg aatgcctcgt tgatcttgcg acggatgctg acctcgttgc cgacgaaggc
     2341 gttcttcaca acctgctggg tgatggtcga cccgcctcgg gtctggtcgg atatgaaatt
     2401 gtcgagggcg gcggaggcga tcgccgagaa gtcgatgccg tcgtgctcga agaagtcggc
     2461 atcctcggcg gcgagcatgg cgttgaccat cacctcaggg atctggtcgt aggggatcgg
     2521 ctcggaattg cgcccgtcgt ggagctcggc gagcacctc
//
